LOCUS       DAZRUF010000001        64447 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZRUF010000001 DAZRUF010000000
VERSION     DAZRUF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.001"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..616
                     /locus_tag="ACHXOL_000001"
     CDS             <1..616
                     /locus_tag="ACHXOL_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR6807100.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
     gene            complement(613..2091)
                     /gene="scpC"
                     /locus_tag="ACHXOL_000002"
     CDS             complement(613..2091)
                     /gene="scpC"
                     /locus_tag="ACHXOL_000002"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR6807101.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(2115..2900)
                     /gene="scpB"
                     /locus_tag="ACHXOL_000003"
     CDS             complement(2115..2900)
                     /gene="scpB"
                     /locus_tag="ACHXOL_000003"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR6807102.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(2911..3906)
                     /gene="meaB"
                     /locus_tag="ACHXOL_000004"
     CDS             complement(2911..3906)
                     /gene="meaB"
                     /locus_tag="ACHXOL_000004"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR6807103.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(3899..6043)
                     /gene="scpA"
                     /locus_tag="ACHXOL_000005"
     CDS             complement(3899..6043)
                     /gene="scpA"
                     /locus_tag="ACHXOL_000005"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR6807104.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(6246..7139)
                     /gene="argP"
                     /locus_tag="ACHXOL_000006"
     CDS             complement(6246..7139)
                     /gene="argP"
                     /locus_tag="ACHXOL_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR6807105.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7569..8228
                     /gene="rpiA"
                     /locus_tag="ACHXOL_000007"
     CDS             7569..8228
                     /gene="rpiA"
                     /locus_tag="ACHXOL_000007"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR6807106.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8484..9716
                     /gene="serA"
                     /locus_tag="ACHXOL_000008"
     CDS             8484..9716
                     /gene="serA"
                     /locus_tag="ACHXOL_000008"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR6807107.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            9945..10004
                     /gene="ibsC"
                     /locus_tag="ACHXOL_000009"
     CDS             9945..10004
                     /gene="ibsC"
                     /locus_tag="ACHXOL_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR6807108.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10336..10395
                     /locus_tag="ACHXOL_000010"
     CDS             10336..10395
                     /locus_tag="ACHXOL_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR6807109.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10496..11098)
                     /locus_tag="ACHXOL_000011"
     CDS             complement(10496..11098)
                     /locus_tag="ACHXOL_000011"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR6807110.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11119..11302)
                     /gene="ssrS"
                     /locus_tag="ACHXOL_000012"
     ncRNA           complement(11119..11302)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHXOL_000012"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11344..11673)
                     /gene="zapA"
                     /locus_tag="ACHXOL_000013"
     CDS             complement(11344..11673)
                     /gene="zapA"
                     /locus_tag="ACHXOL_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR6807111.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            11841..12419
                     /gene="ygfB"
                     /locus_tag="ACHXOL_000014"
     CDS             11841..12419
                     /gene="ygfB"
                     /locus_tag="ACHXOL_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR6807112.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12445..13770
                     /gene="pepP"
                     /locus_tag="ACHXOL_000015"
     CDS             12445..13770
                     /gene="pepP"
                     /locus_tag="ACHXOL_000015"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR6807113.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNESEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            13767..14945
                     /gene="ubiH"
                     /locus_tag="ACHXOL_000016"
                     /gene_synonym="visB"
     CDS             13767..14945
                     /gene="ubiH"
                     /locus_tag="ACHXOL_000016"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR6807114.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            14968..16170
                     /gene="ubiI"
                     /locus_tag="ACHXOL_000017"
                     /gene_synonym="visC"
     CDS             14968..16170
                     /gene="ubiI"
                     /locus_tag="ACHXOL_000017"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR6807115.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16244..16327)
                     /locus_tag="ACHXOL_000018"
     CDS             complement(16244..16327)
                     /locus_tag="ACHXOL_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6807116.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16617..17711
                     /gene="gcvT"
                     /locus_tag="ACHXOL_000019"
     CDS             16617..17711
                     /gene="gcvT"
                     /locus_tag="ACHXOL_000019"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR6807117.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            17735..18124
                     /gene="gcvH"
                     /locus_tag="ACHXOL_000020"
     CDS             17735..18124
                     /gene="gcvH"
                     /locus_tag="ACHXOL_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR6807118.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18243..21116
                     /gene="gcvP"
                     /locus_tag="ACHXOL_000021"
     CDS             18243..21116
                     /gene="gcvP"
                     /locus_tag="ACHXOL_000021"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR6807119.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            21382..22125
                     /locus_tag="ACHXOL_000022"
     CDS             21382..22125
                     /locus_tag="ACHXOL_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR6807120.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(22182..23615)
                     /gene="bglA"
                     /locus_tag="ACHXOL_000023"
     CDS             complement(22182..23615)
                     /gene="bglA"
                     /locus_tag="ACHXOL_000023"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR6807121.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            23660..23971
                     /gene="yqfB"
                     /locus_tag="ACHXOL_000024"
     CDS             23660..23971
                     /gene="yqfB"
                     /locus_tag="ACHXOL_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR6807122.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24135..24794
                     /locus_tag="ACHXOL_000025"
     CDS             24135..24794
                     /locus_tag="ACHXOL_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR6807123.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(24990..25970)
                     /gene="ygfZ"
                     /locus_tag="ACHXOL_000026"
     CDS             complement(24990..25970)
                     /gene="ygfZ"
                     /locus_tag="ACHXOL_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR6807124.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26213..26479
                     /gene="sdhE"
                     /locus_tag="ACHXOL_000027"
     CDS             26213..26479
                     /gene="sdhE"
                     /locus_tag="ACHXOL_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR6807125.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26460..26867
                     /locus_tag="ACHXOL_000028"
     CDS             26460..26867
                     /locus_tag="ACHXOL_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR6807126.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(26907..27428)
                     /gene="fldB"
                     /locus_tag="ACHXOL_000029"
     CDS             complement(26907..27428)
                     /gene="fldB"
                     /locus_tag="ACHXOL_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR6807127.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27540..28436
                     /gene="xerD"
                     /locus_tag="ACHXOL_000030"
     CDS             27540..28436
                     /gene="xerD"
                     /locus_tag="ACHXOL_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR6807128.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28461..29171
                     /gene="dsbC"
                     /locus_tag="ACHXOL_000031"
     CDS             28461..29171
                     /gene="dsbC"
                     /locus_tag="ACHXOL_000031"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR6807129.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            29177..30910
                     /gene="recJ"
                     /locus_tag="ACHXOL_000032"
     CDS             29177..30910
                     /gene="recJ"
                     /locus_tag="ACHXOL_000032"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR6807130.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31001..32099
                     /gene="prfB"
                     /locus_tag="ACHXOL_000033"
     CDS             join(31001..31075,31077..32099)
                     /gene="prfB"
                     /locus_tag="ACHXOL_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR6807131.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32109..33626
                     /gene="lysS"
                     /locus_tag="ACHXOL_000034"
     CDS             32109..33626
                     /gene="lysS"
                     /locus_tag="ACHXOL_000034"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR6807132.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33669..34217)
                     /gene="idi"
                     /locus_tag="ACHXOL_000035"
     CDS             complement(33669..34217)
                     /gene="idi"
                     /locus_tag="ACHXOL_000035"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR6807133.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34272..34343
                     /locus_tag="ACHXOL_000036"
     CDS             34272..34343
                     /locus_tag="ACHXOL_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6807134.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34340..34465)
                     /gene="yqfG"
                     /locus_tag="ACHXOL_000037"
     CDS             complement(34340..34465)
                     /gene="yqfG"
                     /locus_tag="ACHXOL_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR6807135.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34467..35545)
                     /locus_tag="ACHXOL_000038"
                     /pseudo
     CDS             complement(34467..35545)
                     /locus_tag="ACHXOL_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
     gene            35981..37900
                     /gene="ygfT"
                     /locus_tag="ACHXOL_000039"
     CDS             35981..37900
                     /gene="ygfT"
                     /locus_tag="ACHXOL_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR6807136.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            37900..38388
                     /locus_tag="ACHXOL_000040"
     CDS             37900..38388
                     /locus_tag="ACHXOL_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR6807137.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(38457..38807)
                     /gene="ghxQ"
                     /locus_tag="ACHXOL_000041"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             complement(38457..38807)
                     /gene="ghxQ"
                     /locus_tag="ACHXOL_000041"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(38843..39157)
                     /locus_tag="ACHXOL_000042"
     CDS             complement(38843..39157)
                     /locus_tag="ACHXOL_000042"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR6807138.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            complement(39090..42029)
                     /locus_tag="ACHXOL_000043"
     CDS             complement(39090..42029)
                     /locus_tag="ACHXOL_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR6807139.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            complement(42026..42805)
                     /gene="ygfM"
                     /locus_tag="ACHXOL_000044"
     CDS             complement(42026..42805)
                     /gene="ygfM"
                     /locus_tag="ACHXOL_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR6807140.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(42856..44184)
                     /gene="ssnA"
                     /locus_tag="ACHXOL_000045"
     CDS             complement(42856..44184)
                     /gene="ssnA"
                     /locus_tag="ACHXOL_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR6807141.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(44187..47285)
                     /gene="ygfK"
                     /locus_tag="ACHXOL_000046"
     CDS             complement(44187..47285)
                     /gene="ygfK"
                     /locus_tag="ACHXOL_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR6807142.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSS
                     TPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(47607..48185)
                     /gene="mocA"
                     /locus_tag="ACHXOL_000047"
     CDS             complement(47607..48185)
                     /gene="mocA"
                     /locus_tag="ACHXOL_000047"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR6807143.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            48288..49058
                     /gene="yqeC"
                     /locus_tag="ACHXOL_000048"
     CDS             48288..49058
                     /gene="yqeC"
                     /locus_tag="ACHXOL_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR6807144.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            49106..50731
                     /gene="yqeB"
                     /locus_tag="ACHXOL_000049"
     CDS             49106..50731
                     /gene="yqeB"
                     /locus_tag="ACHXOL_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR6807145.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVEWKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILRVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(50772..51704)
                     /gene="arcC"
                     /locus_tag="ACHXOL_000050"
     CDS             complement(50772..51704)
                     /gene="arcC"
                     /locus_tag="ACHXOL_000050"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR6807146.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(51752..53137)
                     /gene="hyuA"
                     /locus_tag="ACHXOL_000051"
     CDS             complement(51752..53137)
                     /gene="hyuA"
                     /locus_tag="ACHXOL_000051"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFR6807147.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(53190..54401)
                     /locus_tag="ACHXOL_000052"
     CDS             complement(53190..54401)
                     /locus_tag="ACHXOL_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR6807148.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(54459..55655)
                     /gene="dpaL"
                     /locus_tag="ACHXOL_000053"
     CDS             complement(54459..55655)
                     /gene="dpaL"
                     /locus_tag="ACHXOL_000053"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR6807149.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(55713..56900)
                     /gene="ygeW"
                     /locus_tag="ACHXOL_000054"
     CDS             complement(55713..56900)
                     /gene="ygeW"
                     /locus_tag="ACHXOL_000054"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR6807150.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            57379..59157
                     /locus_tag="ACHXOL_000055"
     CDS             57379..59157
                     /locus_tag="ACHXOL_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR6807151.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTSASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(59197..59676)
                     /gene="xdhC"
                     /locus_tag="ACHXOL_000056"
     CDS             complement(59197..59676)
                     /gene="xdhC"
                     /locus_tag="ACHXOL_000056"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR6807152.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(59673..60551)
                     /gene="xdhB"
                     /locus_tag="ACHXOL_000057"
     CDS             complement(59673..60551)
                     /gene="xdhB"
                     /locus_tag="ACHXOL_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR6807153.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(60562..62859)
                     /gene="xdhA"
                     /locus_tag="ACHXOL_000058"
     CDS             complement(60562..62859)
                     /gene="xdhA"
                     /locus_tag="ACHXOL_000058"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR6807154.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            63274..64029
                     /gene="actS"
                     /locus_tag="ACHXOL_000059"
     CDS             63274..64029
                     /gene="actS"
                     /locus_tag="ACHXOL_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR6807155.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            64108..64177
                     /locus_tag="ACHXOL_000060"
     tRNA            64108..64177
                     /locus_tag="ACHXOL_000060"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64140..64142,aa:Gly,seq:ccc)
BASE COUNT        15367 a        16097 c        17132 g        15851 t
ORIGIN      
        1 aattgtgatt ggcatagata atacttatcc gacaattatt ttcgatcagt tgattagtct
       61 cggagataag tatgaaggcg tgaccgccca gccagttgag ttcagcgaaa acggcgttat
      121 cgataatctt tttgaccgtc agttagattt tattatctcc ccacagcatg tatctgcacg
      181 agttcaggaa ctggaaaacc tgactatcag tgaattacca ccgctgcgat tgggctttct
      241 cgtttctcgc cgttatgaag agcgtcaaga gcaagagctg cttcaggagc tgccctggct
      301 acaaatgcgc tttcaaaacc gggctaactt cgaagcaatg attgatgcga atatgcgacc
      361 ttgtgggatc aatcccacca ttatttatcg tccctacagt tttatggcca aaatcagcgc
      421 cgtggagcgc ggacattttc tgacggtgat cccgcatttt gcctggcgac tggtgaatcc
      481 ggcaacgctg aaatattttg atgcgccgca caggccgatg tatatgcagg aatatcttta
      541 ttcaatcaga aatcatcgtt ataccgccac gatgcttcag catattgctg aagatcgtga
      601 cgggacaaac cattaaccca gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa
      661 gacgtggcta agatcgtggt gaatatgtcc gccgggcgca ttttccagat agcgatgcag
      721 ataatcccga tacataggat gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg
      781 ggaaagaccg cgtagatcgg cgatcccttg ttcggtgatg atcactttga cgctgtgttc
      841 gctgtgatca acatggctgc acattggcac gacggttgag atcttgcctt ctttagcaat
      901 cgacggagcc ataaagatcg acagatacgc gttgcgttca aaatcaccgc tgccgccgat
      961 gccgttcatc agatcgaccc cggctacgtg cgttgaattt gcatgcccgt aaatatcaaa
     1021 ctccaggccg acgttcagag cgatgacgcc cagacgacgg atgatttccg ggttattgga
     1081 aatctcctgc ggacgcaaca caatgcggct ggcaaagtaa tccatattgt cgtaaatctt
     1141 gcgcagggaa tcggccgaga ttgtcaggct ggaggcgctg gccccgctga ttttgccggt
     1201 ttccagtaaa tgcaccaccg attcctgtag cacttccgaa tacatcatga acggaggaat
     1261 ttccgggttt tcccccagac gcgccattac cgcattattg atattgccca cgccactttg
     1321 cagcggcaga aattccggcg gaatacgccc atgcgccatt tcctgcaata agaacgtgac
     1381 cacgttatcg gcaatctgct ggcacatggg attttgctta tccagcatat taccggcgtc
     1441 gggcaagttg gtttccacga cggcgacaat ctttttcgga tcgatttgca catagcgggt
     1501 accgacgcga tccattgcat ggaagatcga cacgctattg cgccgcggtg gtgcgccagg
     1561 aatcacaata tccgccagtt ctgcaacgcg cggatcgtga tagtgattaa gttcaatgat
     1621 cactttcttc gcccgcagca gccatgtcgg cgcattaccg atcccgctgg ttaaccagac
     1681 tcgaccatcc ggtgccagtg ccgatgcttc gatgacggca acatcaatgt cgccgaagaa
     1741 accgtaattg accatttgcg ccacttcgct caaatgcagg tcaacgaaac tcaccgcgcc
     1801 ctgattgatc tttttacgta aaccggacga tgtttgatat ggcgcacgcc aggaaacagc
     1861 atcggcgtca gaaagtacat cgtcagcggc ggcgctgatt gacgcccccg tcagcaggcg
     1921 aatttgatac ggctttttgg cctcatgctg ttcgttagct ctgcgggcaa tcgcggtcgg
     1981 taaggctttc ggcgaacccg ctggggtaaa gccgctaaat gccaccatgt cgttatgctg
     2041 gataatttct gccgcttcat cggcggtcat ccttgtccac tgagtttcca tttttactcc
     2101 ttcgttcgca gggattaatg accaacgaaa ttaggtttac gtttttcgag gaaagcgttc
     2161 atcccttcct ggtaatcttc gctgtcataa accgcgcggc gcatcccctg aatacgttca
     2221 aattcatcgg agttcatggt gtgcgcttcg cccagtacac gcagctcttc tttgataaca
     2281 gcaatggcta acggcgcttt ctcagagatg tggtgcgcca tttgtaaggt gaaatcttcc
     2341 agttcttcca cttccacaac atggttgagg atgccgacag ccaacgcgcg ctgggcggtg
     2401 attggcgaag cggtaaaaat cagctctttg acaatgtgga agcccgcgtc gcgggtcagg
     2461 ttgtgaatgc cgaccaggtt atacgggacg ccgaggttta caggcatcat tgagaaggtt
     2521 gaggtactgg cggcgatgat cagatcggaa ctcatgatca tttcaaatgc gccaccccaa
     2581 acactacctt ccaccatcga aatgatcggt ttcgggaatt tttggatcat gcgggtgatt
     2641 tgacgcaatg gatcatcata ggagagcgga tcgcgaccgc cagacggcag ttcgtgaata
     2701 tcgtgacctg cggagaagac tttggatcca cttggcgccc gcaaaatgat acagcgaatt
     2761 tccggccggt tgagatcgct taacgcctgc ataagatcat caataaagac tttacttaag
     2821 gcattaagtt ttcgtccata gttaaactca atgaccgcca ctttgttgat agtggcaacg
     2881 ttcacatact gataagacat aaaaattcct ttaatcaaaa tattgcgtct ggataaattc
     2941 actgagctgc cgcaggccgg tgcgcggtga gagcgtattg tttttgaccg ctaaaagcgt
     3001 ctggcggtaa tagcgatcga aatcttcatt cgcgaacagg tgattcagta cttcttcttc
     3061 ggtctgctta cgcagccatt ccaccgattg ttgttgccgc acttgttgta aacgaccact
     3121 ggcagttagc gcggttttga agtcgatgat ggcgtgccag atctcatcga ttccacgttt
     3181 ttccagtgcg ctacaagtca gaacccgtgg ctgccattcg tcgtatttac gtcgcagaat
     3241 atgcagggca ctctcgtaca tatgccgggc aatggcgaca ttggtatggt tatcgccatc
     3301 gtctttgttg ataacgagca gatcagccac ttccatcagc ccttttttaa tgccctgcag
     3361 atcatcgccg ccaccggcaa tttgcaacga gataaaacag tccaccatgc gggcgacttc
     3421 cgtttccgac tgcccgacgc caaccgtttc gacaatcact acgtcataac ccgccgcttc
     3481 gcataacagc attaattccc gtgctcgctg actggcaccg cccagatgac cggaggaggg
     3541 taccgggcga ataaacgccg cctcggcacg cgccaggtca ttcatgcggg ttttatcccc
     3601 gagaatgcta ccgccagtga ccgggctgct gggatcgacc gcaataacag caacctttaa
     3661 tccctcacga atcaacaaca tgccaaaggc ttcaagaaag gtacttttcc ccgcgccggg
     3721 tgtgccggta acgcccagtc gcagggcgtt accacagaac ggcataatgg cgtcaagcag
     3781 ctgtgtactt agcgcctgat gacgtgggtg acggctttcc accagcgtca tggcctgggc
     3841 gagcgtggca cgctcaccct gacgtaagcg gcgaatactt tctgccagcg tggcttcatt
     3901 aatcatgatg ctggcttatc agattcagta cgtcgcgcac actgtcgagc ataggtgtac
     3961 ctggaccata aatcgccgcc acgccgcgct cttgcaggaa ggcgtaatcc tgcggcggaa
     4021 tgacgccacc cgcgaccacg cagatatctt cgcgtcccca ttttttcagc gcttcgacca
     4081 gttccgggat cagcgtttta tgatcggcag ccagtgagga tgcgcccact acgtgaacgt
     4141 cgttttctac ggccaggcgg gcgatctctt caggtgtaga gaacatcggg cttaaatcta
     4201 cgtcgaaacc gagatcggaa taggcgctgg cgatcacttt cgcgccgcga tcgtgtccat
     4261 cctggcccat cttagcgatc agaatgcgcg ggcgacgacc attgtcggca aggaactgct
     4321 ccgtttgcgc aacaatggca tcgaactcgg aggccgattt ctcagactga tgatagcttt
     4381 gcgcaatcac gccggtaaca cactggcttg gcaccagata acggtcgaaa gcgacttcca
     4441 gcgcatcgga aatttcaccc agggtggcgc gaacgcgagc ggcattaaca gcggcagcca
     4501 gcaggttttc gttatgctgt gcggcgtgag tcagggcgtt caacgcggcg gttacggcgg
     4561 catcatcacg ggtggcgcga atgcgttcca gcgaagcaat ttgctcgtta cgcaccatca
     4621 cgttgtcgat ctcaagtaca tcggtttcgt cttcgtgatc cagtttgtac ttgttgacac
     4681 caacgatgac acgcttgcct tggtcgatca gcgactgttc gcgcgctgag gcctcttcga
     4741 tcattcgttt tggcagacct gcttcgatcg ctttcgccat gccaccggct tcgtcgatct
     4801 gttggataat agctctggct tgtttgacga tttgatcggt cagcgactca atgtaatagg
     4861 atccggccag tggatcgacg gtgcggcaga gttctgattc ttcctggatg atgatctggg
     4921 tgttgcgggc aatgcgtgct gagaaatcgg taggcaaacc aagcgcttcg tcaaaggcgt
     4981 tggtatgcag tgactgagta ccgcccagcg tcgcagccag tgcttcaatg gtggtgcgga
     5041 taacgttgtt atacggatcc tgttcagtca ggctccagcc tgaggtctgg cagtgggtac
     5101 gcagcgccag tgatttcgga tcctgtgcgc caaatccact gaccgcttcg ctccataaat
     5161 aacgtgccgc acgcaacatg gcgacgttca taaacagatc catgccgata ccgaagaaga
     5221 acgacaggcg aggagcgaag tcatcaattt tcagtccggc agagattgct gctttgatgt
     5281 actcaatccc atcggcgagc gtaaatgcta cctgctgcac gcagttggca cccgcttccc
     5341 ccatgtggta accgctgata ctgatggtat taaatcgcgg catgttgccg gaacaccagg
     5401 cgatgatgtc ggcgataatg cgcattgacg gttttggtgg gtaaatatag gtgttgcggc
     5461 agaggtactc tttgagaata tcgttttgaa tggtgccggt cagtttatca ggtgtaacac
     5521 cttgctcttc tgcggcgacg atataaaacg ccagtactgg tagcactgcg ccattcatgg
     5581 tcatcgaaac cgacatttta tccagcggga tctggtcgaa caggactttc atatcttcca
     5641 cggtgtcgat agcgacgccc gctttgccga cgtcacccgc cacgcgtggg ttatcggagt
     5701 cgtagccacg gtgggtggca aggtcaaacg caacggaaag acctttttgc ccggcggcca
     5761 ggttacggcg ataaaaagcg ttggactctt ttgctgttga aaaaccagca tactgacgga
     5821 tggtccacgg ttgggcggta tacatagtgg cacgcgggcc acgaacgtag ggcggcaaac
     5881 caggaagggt acctgtcacc tccagattat cgagatcggc ttcggtatac agctgcttga
     5941 tggcgatccc ttccgcggtt tgctgaacca gcgagtcgac agttttctcc cgacggctca
     6001 attccttgtt ggcaagctgt tgccactcct gctcgttaga cattatttgc tccgtaaaac
     6061 gcaatcaaaa tgagggtatt tgcgcttacg gtgagctttt tacgtgggtt tgtcgccagc
     6121 aaaaaaatgc attcagacgt cgttttttgc cgcctgagat ttgtctattt tgtgatgtga
     6181 ttcaaggaat acaaaaagaa gtgaggataa cgcctgatat gtgctatcag gcattatttg
     6241 atggattaat cctgacgaag gactttgtga ccataatcga gcaacgcatc agtgacttta
     6301 cgcatcatgc ggctttcagg agcaaagcgg tgccagtaga gcatccggcg ttgaaatagc
     6361 cctggcgtta agtcaatcag ttcaccgctg gctagttctt tctcgatttg caggtgcggg
     6421 atcatacagc aggtggtgcc ctggcgagca agttgcacga acgcttctga agaattaacg
     6481 atatggcagg gcacgctgcc tggaggcaga tcgaagtttt gctgcaaaaa ggcctggtgc
     6541 atatcgtcaa gatggtcaaa ggcgaccaca ggtgctttca gtaatgccga acgcgttacg
     6601 ccgttaggga aatatttttc ggcaaagggt tttgagctga cgaacagata gtcgagcgca
     6661 ccaagtttat cgacaagaca actcggcagc gcctgatgtt gaatactcac cgcgccgacc
     6721 acttcgccgc ggcgcagacg ttcctgagta cgggtttcat cttctacctg caagttgagg
     6781 cggataggcg aatcagccaa cacaggagcc agtgccggaa gcaaccacgt cgccagactg
     6841 tcggcgttga ccgccagtga aagcagcagc ggagtcgaac cggtttgttc atcgcccagc
     6901 cactcttctt ccagcaactc cacctggcgc agcagtgcca gcagtttttg cccttgttcc
     6961 gtcgggcgcg gcggtacggt acgcaccaac agcggctgcc cgaacatatt ttccagttgc
     7021 ttaatgcgct gtgagacggc tgattgtgta atgcacagct tttgtgccgc gcgctcaaat
     7081 cctcgttcac gtatcaccgc atccagtgcc tgtaatgttc tgtagtccgg gcgtttcatt
     7141 gctgttgtct gctcctggta aatgcttttt cctgcactat gacacaattt tgtgtcaggt
     7201 tgcatatacc gaagccgtcg gcgggtaaca tttgtatccg tggttaaacg cgcagcttaa
     7261 aagatcaggg atttgcgcta atgcatttcg ctcagcgagt tcgtgccctg gttgtgttga
     7321 atggggtggc gttattgcct caatttgcct gtaaacaggg gcttgcgaac ggggggaact
     7381 ggtgcgcctg ttcgcaccgt ggagcggcat acccagaccg ttgtatgctt tatttgcggg
     7441 gcgaaagggg atgcccgcca ttgcgcgata ttttatggat gagttaacca cgcggcttgc
     7501 caacggggtc tgaatcgctt tttttgtata taatgcgtgt gaaatttcat accacaggcg
     7561 aaacgatcat gacgcaggat gaattgaaaa aagcagtagg atgggcggca cttcagtatg
     7621 ttcagcccgg caccattgtt ggtgtaggta caggttccac cgccgcacac tttattgacg
     7681 cgctcggtac aatgaaaggc cagattgaag gggccgtttc cagttcagat gcttccactg
     7741 aaaaactgaa aagcctcggc attcacgttt ttgatctcaa cgaagtcgac agccttggca
     7801 tctacgttga tggcgcagat gaaatcaacg gccacatgca aatgatcaaa ggcggcggcg
     7861 cggcgctgac ccgtgaaaaa atcattgctt cggttgcgga aaaatttatc tgtattgctg
     7921 acgcttccaa gcaggttgat attctgggca aattcccgct gccagtagaa gttatcccga
     7981 tggcacgtag tgcagtggcg cgccagctgg tgaaactggg cggtcgtccg gaataccgtc
     8041 agggcgtggt gaccgataac ggcaacgtga tcctcgacgt ccacggcatg gaaatccttg
     8101 acccgatagc gatggaaaac gccataaatg cgattcctgg cgtggtgact gttggcttgt
     8161 ttgctaaccg tggcgcggac gttgcgctga ttggcacacc tgacggtgtc aaaaccattg
     8221 tgaaatgatc tgacggggga acctcccccg ttaaaaaaat tctcttcatt aaatttggtg
     8281 acatgtgtca cgtttttact tggctattgt cgattgctct aaataaatcc tctaaaccag
     8341 catattcaac caagaaatac ctttgcgtga tatttcctca acatcgcgac gcaaacgttc
     8401 atattgccgc aatattattt tttgatatgt tgaaaggcgg atgcaaatcc gcacacaaca
     8461 tttcaaaaga caggattggg taaatggcaa aggtatcgct ggagaaagac aagattaagt
     8521 ttctgctggt agaaggcgtg caccaaaagg cgctggaaag ccttcgtgca gctggttaca
     8581 ccaacatcga atttcacaaa ggcgcgctgg atgatgaaca attaaaagaa tccatccgcg
     8641 atgcccactt catcggcctg cgatcccgta cccatctgac tgaagacgtg atcaacgccg
     8701 cagaaaaact ggtcgctatt ggctgtttct gtatcggaac aaaccaggtt gatctggatg
     8761 cggcggcaaa gcgcgggatc ccggtattta acgcaccgtt ctcaaatacg cgctctgttg
     8821 cggagctggt gattggcgaa ctgctgctgc tattgcgcgg cgtgccggaa gccaatgcta
     8881 aagcgcaccg tggcgtgtgg aacaaactgg cggcgggttc ttttgaagcg cgcggcaaaa
     8941 agctgggtat catcggctac ggtcatattg gtacgcaatt gggcattctg gctgaatcgc
     9001 tgggaatgta tgtttacttt tatgatattg aaaacaaact gccgctgggc aacgccactc
     9061 aggtacagca tctttctgac ctgctgaata tgagcgatgt ggtgagtctg catgtaccag
     9121 agaatccgtc caccaaaaat atgatgggcg cgaaagagat ttcgctaatg aagcccggct
     9181 cgctgctgat taatgcctcg cgcggtactg tggtggatat tccggcgctg tgtgatgcgc
     9241 tggcgagcaa acatctggcg ggggcggcaa tcgacgtatt cccgacggaa ccggcgacca
     9301 atagcgatcc atttacctct ccactgtgtg aattcgacaa cgtccttctg acgccacaca
     9361 ttggcggttc gactcaggaa gcgcaggaga atatcggcct ggaagttgcg ggtaaattga
     9421 tcaagtattc tgacaatggc tcaacgctct ctgcggtgaa cttcccggaa gtctcgctgc
     9481 cactgcatgg tggtcgtcgt ctgatgcaca tccacgaaaa ccgtccgggc gtgctaactg
     9541 cgctgaacaa aatcttcgcc gagcagggcg tcaacatcgc cgcgcaatat ctacaaactt
     9601 ccgcccagat gggttatgta gttattgata ttgaagctga cgaagacgtt gccgaaaaag
     9661 cgctgcaggc aatgaaagct attccgggta ccattcgcgc ccgtctgctg tactaattcc
     9721 ccttctctga aaattaacgg gcaggtcact gacttgcccg tttttttatc ccttctccac
     9781 accgttgccg cgttgcgcat tctactttgc gagtctgctc cagaaaataa atcaaactca
     9841 gatcaatgcg ttgtgtttat ttaatgggga tttacgatgg cgggacagca tggggctgta
     9901 acggtaaagc cctcaccgaa gcgagggctt aaaggagaag ggttatgatg cgacttgtca
     9961 tcatactgat tgtactgtta ctgataagtt tcggagctta ttaacagtca gtctcagggg
    10021 aggagtcatc ctctcttacc cttactcact aaattaggtc aaagaatcaa cgatgtcaat
    10081 cagggcgatg cggggtgcat cgccctgaac cgaacttttt gatagccact tcatcgccca
    10141 cgtttactgc cgtaatagat cctttttccc aaaaattatc tctgttttgc atgggttttt
    10201 cgagcacgct cgaagaaaag atttaaaggt agatcaatgc gttgtggtta tttaaagaga
    10261 gcttacgata gctgggcagc atggggctgt aacggtaaag ccctcaccga agcgagggct
    10321 tgaaggagaa gggttatgat gcgacttgcc atcatactga ttgtactgtt actcataagt
    10381 ttcccagctt attaacagtc agtcacaggg gaggagcaat cctcccttac ccttactcac
    10441 taaattaggt taaagaatca acgatgtcaa tcagggcgat gcaggtgtat cgcccttacc
    10501 actcccagac tttcgacggt gtaaccaccg caggaagagg gatatcccac tcttcaacgg
    10561 ggagtttttc caccaactga caatcatgcg cataacccac cggttgcgtt ttatagtgct
    10621 gccagttttg taaggtccga tcataaaaac caccgcccat tcccaggcgc tgaccgtact
    10681 catcaaaggc gaccagcggt gtgatcagca cgtctaatcg ggaaaggggt agcacgtcac
    10741 gcacatccag ttttggctca tggatcttca acctgttcat caccagttcg ctttgcggat
    10801 ggtaattcag gaacagcaaa ttaccggcac taaagggatg caaaactgga agatatacgc
    10861 gcttaccggc gcgccagagt tgttctatta gcggctgggt gtcgagttca ccatcaaaag
    10921 agaggaatac agcgaccgta tgcgccatca ccaccggggg ataagtcatc atccgggtag
    10981 cggcttgttg acccatttcc tgctgttgtt ccggcgttaa cgcacgacga cgttgccgaa
    11041 tcattttgcg gatttcttgt cgggataatg tcagtgggag ttctggtagt tgcgtcatgg
    11101 ctggtgccag ataagaaggg aatctccgag atgccgccgc aggctgtaac ccttgaaccc
    11161 ttggttcaag gtgaatgtgt cgtcgcagtt ttaaggcttc tcggacggac cgagcatgct
    11221 caccaaccgc ggagcgccac attcttgtgg tatgaaatat cggctcaggg gactggcccg
    11281 cttgcgaaca tctcagagaa attttgtctt cacggttact ctaccacagt aaaccgaaaa
    11341 gtgtcattca aagttttggt tagttttttc ggtgatgcga ccttgttcaa gtaacgcttg
    11401 ttctatggtc tgctgcagca tccgaatacg ctgttccata cttgccgcgt agtcacgagt
    11461 cttcgctttt tcttgcgcta actcatagct gatattcaat gcggcaatga agaccaactg
    11521 ttcagtattt gtgactctag tgcgttcttt cagatcttgc aaccgttggt tcagatcgtc
    11581 cgctgcctga ttcaacgcat ccctttggtc aggcgggcag ttcacacgca gtgaacggcc
    11641 aaaaatttgg atatcgacgg gttgtgcaga catgccacct tcctgctgat tgactgcgct
    11701 gccttcgtct ttagacccat ggtctgcgaa ggggcgacac tatagctacc ctgatgagaa
    11761 gagacaagcc cttttctggt ccaccagggc ccaaagtggt agcatatcat gaatattcct
    11821 ccctttgacg acgaatgctt atgtctatac agaacgaaat gcctggttac aacgaaatga
    11881 accagtatct gaaccaacaa gggacggggc tgacccctgc cgagatgcat ggtttaatca
    11941 gtgggatgat atgtggcggt aacgatgaca gctcatggct gccgctactt cacgacctga
    12001 cgaacgaagg catggctttc ggtcatgagc tggcacaggc actgcgtaaa atgcactctg
    12061 ccaccagcga tgccctgcag gatgacggct tcctttttca gctttatctg cctgatggcg
    12121 atgatgtcag cgttttcgat cgggctgatg cattggcagg ttgggtcaat cacttcctgc
    12181 ttggtcttgg cgttacgcaa ccgaagctgg ataaagtgac cggcgaaacc ggtgaagcta
    12241 tcgacgatct gcgtaacatt gcgcaactgg gttacgacga agacgaagat caggaagagc
    12301 ttgaaatgtc gcttgaagag atcatcgaat acgttcgtgt tgccgcgctg ttatgccacg
    12361 acacctttac tcatccgcaa ccgaccgcgc cagaagtaca aaaaccgact ctacactaaa
    12421 aacaaaaaac gtaaggagag tgttatgagt gagatatccc ggcaagagtt tcagcgtcgc
    12481 cgtcaggccc tggtggagca aatgcaaccc ggcagcgccg cgctgatttt tgctgcacca
    12541 gaagtaacac gtagcgccga cagcgaatac ccctatcgtc agaacagtga cttctggtac
    12601 ttcaccggct ttaacgaatc ggaagcggtg ctggtgctga ttaaaagcga tgacactcat
    12661 aaccacagcg ttctgtttaa ccgcgttcgc gacctgacgg cggagatctg gtttggccgt
    12721 cgcttaggcc aggatgccgc gccagagaaa ctgggcgttg accgcgcact ggcattcagc
    12781 gaaatcaatc agcaacttta tcaactactt aacggcctgg atgtggttta ccatgcccag
    12841 ggcgaatatg catatgctga tgaaatcatg aacagtgcgc tggaaaaact gcgtaaaggt
    12901 tcgcggcaaa atctcaccgc accggcaacg atgatcgact ggcgtcctgt tgttcatgaa
    12961 atgcgcctgt tcaaatcgcc agaagagatt gccgtactcc gccgcgcggg agaaatcacc
    13021 gccatggcac atacacgggc gatggaaaaa tgccgtccgg gaatgttcga gtaccatctg
    13081 gaaggcgaaa ttcaccacga atttaaccgc cacggtgcgc gctatccgtc ctataacacc
    13141 attgtcggca gcggtgaaaa cggctgcatt ctgcactaca ccgaaaacga gagtgaactg
    13201 cgcgacggtg acctggtgtt gattgacgcg ggttgtgaat acaaaggtta cgcgggcgat
    13261 attacccgca ccttcccggt caacggcaaa ttcacccagg cccagcgtga aatctacgac
    13321 attgtgctgg agtctctcga aaccagcctg cgcctgtatc gtccgggaac ttccattctg
    13381 gaagtcactg gtgaagtggt gcgcatcatg gttagcggcc tggtaaaact cggcatcctg
    13441 aaaggtgatg ttgatgaact gatcgctcag aacgcccatc gtcctttctt tatgcatggc
    13501 cttagccact ggttaggact ggatgtccat gacgttggcg tttatggtca ggatcgctcg
    13561 cgcattctgg aaccggggat ggtactgacc gtagagcctg gtctgtatat cgcgccggat
    13621 gcggatgtgc cagaacaata tcgcggtatc ggcattcgta ttgaagacga cattgtgatt
    13681 accgaaaccg gcaacgaaaa cctcaccgcc agcgtggtga aaaagccgga agaaatcgaa
    13741 gcgttgatgg cagctgcgag aaagcaatga gcgtaatcat cgtcggtggc ggcatggcgg
    13801 gcgcgacgct ggcgctggct atttcccggt taagtcacgg ggcgctgccg gtacatttga
    13861 ttgaagcgac cgcgccagag tcacatgctc atccgggctt tgatggacga gctattgcgc
    13921 tggctgcggg gacctgtcag caactggcgc gcatcggcgt ctggcaatct ctggcggatt
    13981 acgcaaccgc catcactacc gtgcatgtca gcgatcgtgg tcacgctgga tttgtcaccc
    14041 tcgccgcaga agattaccaa ctggcggcgc tgggacaggt tgtcgaattg cacaatgtcg
    14101 ggcaacggct gtttgcattg ctgcgtaaag cacctggcgt aacgctgcat tgccctgatc
    14161 gcgtggctaa cgttgcccgt actcagagtc acgttgaagt gacgctggag agtggcgaga
    14221 cgctgacggg ccgcgtgctg gtagcagctg atggcaccca ttcagcgtta gccaccgcgt
    14281 gcggcgttga ctggcagcag gagccttacg aacaactggc cgtgattgcc aacgttgcga
    14341 cttccgttgc gcacgaaggc cgcgcttttg aacgcttcac gcaacatggc ccgctggcga
    14401 tgttgccgat gtctgacgga cgctgttcgc tggtctggtg tcatccactg gaacggcgcg
    14461 aagaggtgct gtcgtggagc gacgagaagt tttgccgtga actccagtcg gcctttggct
    14521 ggcgacttgg aaaaattacc cacgccggta aacgcagtgc ttatccgctg gcgttaaccc
    14581 gcgccgccag agctattacc catcgtaccg tgctggtggg caatgcggcg caaactctgc
    14641 acccgattgc cgggcaaggg tttaacctcg gtatgcgaga tgtcatgagc cttgcggaaa
    14701 ccctgactca ggcgcatgag cgcggagaag acatcggtga ttacggcata ttgtgccgct
    14761 atcagcagcg tcgacagagc gatcgcgaag caaccatcgg cgtcacggac agcctggtac
    14821 atctttttgc caaccgttgg gcaccgctgg ttgtcgggcg caacatcgga ctgatgacga
    14881 tggaattatt caccccggca cgcgatgtgc tggcgcagcg caccctcggt tgggtggcgc
    14941 gttgaaggcg gttttaagga ataaacaatg caaagtgttg atgtagccat tgttggtggc
    15001 ggcatggtgg ggctggcggt tgcctgcggc ttacagggga gcggcttacg cgttgccgta
    15061 ctggagcagc gcgtaccgga acctctggcg gcggatgcac caccacaact gcgcgtttcg
    15121 gctatcaatg ccgccagcga aaaattactc acccgtcttg gcgtctggca ggacattctc
    15181 tctcgtaggg ccagttgtta tcacggtatg gaagtgtggg acaaagacag ttttggtcac
    15241 atttcgtttg acgatcaaag catgggctat agccatcttg ggcatatcgt tgaaaactca
    15301 gtgattcact acgcgctgtg gaacaaagcg cagcagtcgt cagatatcac tctgttggcc
    15361 cccgcagaat tacagcaggt cgcctgggga gaaaatgaaa ctttcctgac gctgaaagat
    15421 ggcagcatgt taacggcgcg tctggtgatt ggcgcggacg gtgctaattc ctggttgcgc
    15481 aacaaagcag atattccgct gactttctgg gattatcagc atcacgcgct ggtagcgacc
    15541 attcgcacgg aagaaccgca tgatgcggtg gcgcggcagg ttttccatgg cgaaggcatt
    15601 ctggcctttt taccgcttag cgatccgcat ctttgctcaa ttgtctggtc actgtcgcca
    15661 gaggaagcgc agcggatgca gcaggcaagt gaagatgaat ttaatcgcgc gttaaatatc
    15721 gcttttgata atcgcctggg cttatgcaag gttgagagcg cgcgtcaggt gttcccactg
    15781 acggggcgtt atgcgcgcca gtttgccgcg caccgtctgg tgctggtggg cgacgccgcg
    15841 cataccattc acccgctggc ggggcaggga gtaaatcttg gctttatgga tgctgcagag
    15901 ctgattgctg aactgaaacg gttgcatcgt caggggaaag acatcgggca gtacatttac
    15961 ctgcgccggt atgagcgtag ccgcaagcac agtgcggcgc tgatgctggc tggtatgcag
    16021 ggattccgcg atctgttttc cggtaccaat ccggcgaaaa aactgctgcg tgatattggt
    16081 ttgaaactgg ccgacacgct tcctggcgtt aaaccacaac ttatccgcca ggcaatggga
    16141 ttaaacgatt tgcctgaatg gctgcgttaa aaatttctct tctgctgttt atttgatacc
    16201 catcacactt tcatctcccg gttttttcgc cgggagattt tcctcatttg aaataaacta
    16261 atttcacctc cgttttcgca ttatattttc taatgtcatt attttttgat ttagtgtttt
    16321 ttgacatttt tttagctctt aatattgcct tattcaaatt agctttctaa tggcatcatc
    16381 tttatataga aactggtgtg ttttttggtt ttttattctg tcgcgatttt ggcgtttttt
    16441 aaccataagc taatgtgatg atcaatttta ccttatggtt aacagtctgt ttcggtggta
    16501 agttcaggca aaagagaacg attgcgttgg ggaccgggag tggctccgat gctgggtttc
    16561 gtggtgataa tttcaccatg aaaagttgtc agccccgctt attcaatgag gacaagatgg
    16621 cacaacagac tcctttgtac gaacaacaca cgctttgcgg cgctcgcatg gtggatttcc
    16681 acggctggat gatgccgctg cattacggtt cgcaaatcga cgaacatcat gcggtacgta
    16741 ccgatgccgg aatgtttgat gtgtcacata tgaccatcgt cgatctccgc ggcagccgca
    16801 cccgggagtt tctgcgttat ctgctggcga acgatgtggc gaagctcacc aaaagcggca
    16861 aagcccttta ctcggggatg ttgaatgcct ctggcggtgt gatagatgac ctcatcgtct
    16921 actactttac tgaagatttc ttccgcctcg ttgttaactc cgccacccgc gaaaaagacc
    16981 tctcctggat tacccaacac gctgaacctt tcggcatcga aattaccgtt cgtgatgacc
    17041 tttccatgat tgccgtgcaa gggccgaatg cgcaggcaaa agctgccaca ctgtttaatg
    17101 acgcccagcg tcaggcggtg gaagggatga aaccgttctt tggcgtgcag gcgggcgatc
    17161 tgtttattgc caccactggt tataccggtg aagcgggcta tgaaattgcg ctgcccaatg
    17221 aaaaagcggc cgatttctgg cgtgcgctgg tggaagcggg tgttaagcca tgtggcttgg
    17281 gcgcgcgtga cacgctgcgt ctggaagcgg gcatgaatct ttatggtcag gagatggacg
    17341 aaactatttc tcctttagcc gccaacatgg gctggactat cgcctgggaa ccggcagatc
    17401 gtgactttat cggtcgtgaa gccctggaag tgcagcgtga gcatggtaca gaaaaactgg
    17461 ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga actgccggta cgctttaccg
    17521 atgcgcaggg caaccagcat gaaggcatta tcaccagcgg tactttctcc ccgacgctgg
    17581 gttacagcat tgcgctggcg cgcgtgctgg aaggtattgg cgaaacggcg attgtgcaaa
    17641 ttcgcaaccg tgaaatgtcg gttaaagtga caaaacctgt ttttgtgcgt aacggcaaag
    17701 ccgtcgcgtg atttactttt ttggagattg attgatgagc aacgtaccag cagaactgaa
    17761 atacagcaaa gaacacgaat ggctgcgtaa agaagccgac ggcacttaca ccgttggtat
    17821 taccgaacat gctcaggagc tgttaggcga tatggtgttt gttgacctgc cggaagtggg
    17881 cgcaacggtt agcgcgggcg atgactgcgc ggttgccgaa tcagtaaaag cggcgtcaga
    17941 catttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac gacgcactga gcgattcccc
    18001 ggaactggtg aacagcgaac cgtatgcagg tggctggatc tttaaaatca aagccagcga
    18061 tgaaagcgaa ctggaatcac tgctggatgc gaccgcatac gaagcattgt tagaagacga
    18121 gtaacggctt tattcctctt ctgcgggaga ggatcagggt gaggaaaatt tatgcctcac
    18181 cctcactctc ttcgtaagga gagaggttca caattcactg cacgtttcag gaaccatcgc
    18241 tcatgacaca gacgttaagc cagcttgaaa acagcggcgc ttttattgaa cgccatatcg
    18301 gaccggacgc cgcgcaacag caagaaatgc tgaatgccgt tggcgcacaa tcgttaaacg
    18361 cgctgaccgg ccagattgta ccgaaagata ttcagcttgc gaccccgccg caggttggcg
    18421 caccggcgac cgaatacgcc gcactggcag aactcaaggc tattgccagt cgcaataaac
    18481 gcttcacgtc ttacatcggc atgggttaca ccgccgtgca gctaccgccg gttatcctgc
    18541 gtaacatgct ggaaaatccg ggctggtata ccgcgtacac tccgtatcaa cctgaagtct
    18601 cccagggccg ccttgaagca ctgctcaact tccagcaggt aacgctggat ttgactggac
    18661 tggatatggc ctctgcttct cttctggacg aggccaccgc tgccgccgaa gcaatggcga
    18721 tggcgaaacg cgtcagcaaa ctgaaaaatg ccaaccgctt cttcgtggct tccgatgtgc
    18781 atccgcaaac gctggatgtg gtccgtactc gtgccgaaac ctttggtttt gaagtgattg
    18841 tcgatgacgc gcaaaaagtg ctcgaccatc aggacgtctt cggcgtgctg ttacagcagg
    18901 taggcactac cggtgaaatt cacgactaca ctgcgcttat tagcgaactg aaatcacgca
    18961 aaattgtggt cagcgttgcc gccgatatta tggcgctggt gctgttaact gcgccgggta
    19021 aacagggcgc ggatattgtt tttggttcgg cgcaacgctt cggcgtgccg atgggctacg
    19081 gtggcccaca cgcggcattc tttgcggcga aagatgaata caaacgctca atgccgggcc
    19141 gtattatcgg tgtatcgaaa gatgcagctg gcaataccgc gctgcgcatg gcgatgcaga
    19201 ctcgcgagca acatattcgt cgtgagaaag cgaactccaa catttgtact tcccaggtac
    19261 tgctggcaaa catcgccagc ctgtatgccg tttatcacgg cccggttggc ctgaaacgta
    19321 tcgctaaccg cattcaccgt ctgaccgata ttctggcggc gggcctgcaa caaaaaggtc
    19381 tgaaactgcg ccatgcgcac tatttcgaca ccttgtgtgt ggaagtggcc gacaaagcgg
    19441 gcgtactgac gcgtgccgaa gcggctgaaa tcaacctgcg tagcgatatc ctgaacgccg
    19501 tggggatcac ccttgatgaa accaccacgc gtgaaaacgt aatgcagctt ttcagcgtgc
    19561 tgctgggcga taaccacggg ctggagatcg acacgctgga caaagacgtg gctcacgaca
    19621 gccgctctat ccagcctgcg atgctgcgcg acgacgaaat cctcacccat ccggtgttta
    19681 atcgctacca cagcgaaacc gaaatgatgc gctatatgca ctcgctggag cgtaaagatc
    19741 tggcgctgaa tcaggcgatg atcccgctgg gttcctgcac catgaaactg aacgccgccg
    19801 ccgagatgat cccaatcacc tggccggaat ttgccgaact gcacccgttt tgcccgccgg
    19861 aacaggctga aggttatcag cagatgattg cgcagctggc tgactggctg gtgaaactga
    19921 ccggttacga cgccgtttgt atgcagccga actccggcgc acagggcgaa tacgcgggcc
    19981 tgctggcgat tcgtcattat catgaaagcc gcaacgaagg gcatcgcgat atctgcctga
    20041 tcccggcttc tgcgcacgga actaaccccg cttctgcaca tatggcagga atgcaggtgg
    20101 tggttgtggc gtgtgataaa aacggcaaca tcgatctgac tgatctgcgc gcgaaagcgg
    20161 aacaggcggg cgataacctc tcctgtatca tggtgactta tccttctacc cacggcgtgt
    20221 atgaagaaac gatccgtgaa gtgtgtgaag tcgtgcatca gttcggcggt caggtttacc
    20281 ttgatggcgc gaacatgaac gcccaggttg gcatcacctc gccgggcttt attggcgcgg
    20341 acgtttcgca cctcaacctg cataaaactt tctgcattcc gcacggcggt ggtggtccgg
    20401 gtatgggacc gatcggtgtg aaagcgcatc tggcaccgtt tgtaccgggt catagcgtgg
    20461 tgcaaatcga aggcatgtta acccgtcagg gcgcagtttc tgcggcaccg ttcggtagcg
    20521 cctctatcct gccaatcagc tggatgtaca tccgcatgat gggcgcagaa gggctgaaaa
    20581 aagcaagcca ggtggcaatc ctcaacgcca actatattgc cagccgcctg caggatgcct
    20641 tcccggtgct gtataccggt cgcgacggtc gcgtggcgca cgaatgtatt ctcgatattc
    20701 gcccgctgaa agaagaaacc ggcatcagcg agctggatat tgccaagcgc ctgatcgact
    20761 acggtttcca cgcgccgacg atgtcgttcc cggtggcggg tacgctgatg gttgaaccga
    20821 ctgaatctga aagcaaagtg gaactggatc gctttatcga cgcgatgctg gctatccgcg
    20881 cagaaattga ccaggtgaaa gccgctgtct ggccgctgga agataacccg ctggtgaacg
    20941 cgccgcacat tcagagcgaa ctggtcgccg agtgggcgca tccgtacagc cgtgaagttg
    21001 cggtattccc ggcaggcgtg gcagacaaat actggccgac ggtgaaacgt ctggatgatg
    21061 tttacggcga ccgtaacctg ttctgctcct gcgtaccgat tagcgaatac cagtaattca
    21121 ctgattcgac tattttctaa aggcgcttcg gcgccttttt agtcagatga caaagtacaa
    21181 aagtgctcag acagtcccct cgcccctttg gggagagggt tagggtgagg ggaacaggcc
    21241 ggcactggcg cgaatattta ccctcacccc ggccctctcc ctgaaagggc gaggggaaaa
    21301 gcgtgccaac attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat
    21361 taatttttct cggaaaaaca aatggctata gcacttgtga ccgatggcag tcgcggcatc
    21421 gggcgggcaa ctgcattact gttggcgaaa gaagagtata cggtggcggt taattatcaa
    21481 caaaatctcc atgcggcgca ggaagtggtg aacttaatca cgcaagccgg tggcaaggca
    21541 ttcgtgctcc aggcggatat cagcgacgaa aatcaggtca tcgcaatgtt tacagcaatc
    21601 gatcagcacg atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag
    21661 tgcaccgttg aaaaccttac cgcagagcga atcaaccgag tactttccac caacatgacg
    21721 ggatattttc tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt
    21781 ggcggcgcta tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat
    21841 gttgattacg cggcatcgaa aggggcgatt gatacgttaa ccaccggact atcgctggaa
    21901 gtcgccgcgc agggggtccg cgttaactgc gtgcggccag ggtttatttt taccgaaatg
    21961 cacgccagcg gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag
    22021 cgtggtggac aggcagaaga ggtcgcgcag gccattgtct ggctactaag tgataaagcc
    22081 tcttacgtca cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc
    22141 tgaccggatg caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg
    22201 ctggtaatca cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca
    22261 gtaccgtcgt catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggtg
    22321 gtgaacgata cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacgccatcg
    22381 taagtcaccg cttttttcat ctcttcgata tgggcgcgca ggtagtcaat gcggtagtcg
    22441 tcgttgatgc tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata
    22501 aacagcggcc tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga
    22561 tcaatctgcc agccccagtc agacgcttta acatacgggt tcggtacgct gccttcgaaa
    22621 ccagagatcg catcgccggt gccgccttcg gccttcactg cgttggtcat gtaatagctg
    22681 aaaccaagat aatcgcaggt gccttcgcgc agtacatcga gatcgccgtc ttccattttg
    22741 atgttaaatc cgcgacgctc ccactcgttc aacacatagg acgggtaata gccgcgtagc
    22801 tgcacatcgg taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc
    22861 gggttacagc agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc
    22921 gggttaatgc gacgcgcagc tttcaccgcc agggcgctgg cgacaaactg gtgatgcagc
    22981 acctgataca tcgtctcttc cgggttctca tgctcggtat aaaccacgcc ggagcagcag
    23041 taaccgaaca gcggtgcacg ccagttacgc tggttgttaa tttcgttgaa ggtcatccag
    23101 tatttgactt tatgcttata gcgttcaaac accacttccg cgaaacggac aaagaaatca
    23161 accactttac ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag
    23221 tgggagaggg tgatcaccgg ttcgatgttg tatttcagca gttcatcgaa catatcatcg
    23281 tagaacttca gcccttcttc gtttggctga gcttcatcgc cttttggaaa aatgcgcgtc
    23341 caggctatgg aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc
    23401 ttgtagtgac cataaaaatc aacggcttca tggtttggat agtatttgcc tggcaagact
    23461 tctttggtga tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc
    23521 ggccctttgc cgcctttgtt ccagccgcct tcgacctgat gagcggcaac tgcgccgccc
    23581 cataagaaat ctttctgtaa ggtgagtttt ttcactatca taggctccag atgagtcaat
    23641 tcttacagga aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttccagga
    23701 tgacattctg gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa
    23761 aacgggtgat gtgcttcgtg tcgggcgttt tgaagatgac ggttattttt gcacgattga
    23821 agtcaccgca acctcaaccg taacgctgga tacgctgacg gaaaaacatg cagagcagga
    23881 aaatatgacc ctgactgaac tgataaaggt cattgccgac atctatcccg gtcagacaca
    23941 gttttatgtg attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt
    24001 cgcatttaat ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac
    24061 aggtgttcac tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt
    24121 ctccggagta agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg
    24181 aaattgccaa cagcgtcagt cacggcattg ggttggtgtt tggtatcgtt gggctggtgt
    24241 tgctactggt tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc
    24301 tctatggcgg cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc
    24361 atcaacgggc aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg
    24421 ccggaaccta cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga
    24481 tgattgttat ctggagcctg gcattgctgg gtattctgtt taaactgacc atcgcgcacc
    24541 gattcaaaat tttatctctg gtgacctatc tggcgatggg ctggctgtcg ctggtggtaa
    24601 tttatgaaat ggcagttaag ctcgcggcgg gcagcgttac cttattggcg gtaggcggcg
    24661 tggtttattc gctcggagtg attttctatg tctgcaaacg cattccctac aatcatgcca
    24721 tctggcacgg cttcgtgctc ggcggcagtg tgtgccactt tctggcgatc tatttgtata
    24781 ttgggcaggc gtaatgggta tgcctttgta ggcctgaaaa gacgcgtcag cgtcgcatca
    24841 ggcatcggag cacaactgcc ggattcggcg tgaacgcctt atccgatcta cgggcctcgt
    24901 ccctgttgtg ataaaggcat caaatcccag acatgataag acgcgtcagc gtcgcatcag
    24961 gcgataaatg cctgatgcga taaagagatt tactcttcga gcgaatacgg cagcggctca
    25021 atatgcaatg tattcgcatc gtcgcgtacg cggaagatgc tatccggttc catatcgtta
    25081 ttcatgacga cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcaccgta
    25141 ccggtacgac gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt
    25201 cggctggcgc tacctgccag caaccagagc gcacgtttat tggcaccacg gaattttgct
    25261 cgcgccacca tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc
    25321 tggagattgg tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa
    25381 cccgcttcaa tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc
    25441 agtttatcgg tcagcatatt agcagtagct tcatcggtta cgatcaggaa acgttctgcc
    25501 gggtattcaa accatagcaa agtggtcgcg ccttctttaa ctacctgttt ttctttcgaa
    25561 ggcagttcac taaagatatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc
    25621 agcacatgct cgtcgtctgg cgcgatggtc actttagaga acaccgcata ttttttcagt
    25681 tcagtcagct gcggttcacg cacgctgcgc cgttcaatcc acgcaaagcc atcgccgtcg
    25741 cggaacagac gtaaattgct ccacattttg cctttggcgt cgcaatgggc ggcgagcagg
    25801 tgctgatctt ctgccatctg gctgacatct gctgtcacct gaccctgcat atatttttcg
    25861 ctgtccgcgc cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt
    25921 aaacgggcag aagccgtagg ctgacgggga ggaaaaggtg taaaagccat aatagagtcc
    25981 tgagttgctt aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg
    26041 ctttcactac gcgatctgtg ccttctgcga ggcgacttcc aaaaaaatga agccattgaa
    26101 ttgttgtgaa ttaatttcgt gatcgcgatg gtgtttactg atattcccgt aaaagaaggc
    26161 gaaaaacacg ttacaatagc ggggtactgg attttcgtga gacaggaaga agatggacat
    26221 taacaacaaa gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc
    26281 aatcatgccg tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt
    26341 tattcgtctg ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa
    26401 accggccgat gcagaactgg aaatgatggt ccgactcatc cagacacgga accgggaacg
    26461 tggtcctgtg gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga
    26521 ttcatgggct ggttgccgct gttattttac tcatgccctg gccactcagt tacaccccgt
    26581 tatggatggt gttactttcg ctggtggtgt ttgattgcgt tcgcagccag cggcgtatta
    26641 atgctcgcca gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg
    26701 agtggagcat cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt
    26761 ctgatggcgg taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat
    26821 ggcgggattt acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa
    26881 gcatatgcgc taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt
    26941 tgctcgcacc agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc
    27001 gccagaccca cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat
    27061 ccttccgttg gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc
    27121 ataccgagcg catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc
    27181 gcaacaattt taccttcaag gttcaggtcg tcgagctgat cccagacggc ttcccagtct
    27241 tcctggattt caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc
    27301 attaatttcg gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata
    27361 tcgcggattt tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc
    27421 atattcataa cgtaagcttc tcgattctgc tgtggctttt gcagctagtg tatcagaatc
    27481 gctttagtca cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag
    27541 tgaaacagga tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc
    27601 tggctgaaaa tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc
    27661 atcaccgcgg gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag
    27721 aacggctgga gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc
    27781 gattgttcca gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg
    27841 cttcaccgaa attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt
    27901 tattacaggc gccattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag
    27961 tgttgtatgc taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca
    28021 gcctgcgtca gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtaccgt
    28081 taggtgaaga ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc
    28141 tgaatggtgt gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac
    28201 agaccttctg gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc
    28261 tgtcaccgca tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt
    28321 tacgcgtggt gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc
    28381 atgtcgctac cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc
    28441 tgaaaagaac gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt
    28501 tttcaggttt tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca
    28561 tcaaaagcag cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca
    28621 gcggcgtgtt gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg
    28681 ttagcggcac ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc
    28741 ttgaaaaaga gatgatcgtt tataaagcgc tgcaggaaaa acacgtcatc accgtgttta
    28801 ctgatattac ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc
    28861 tggggatcac cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga
    28921 aagaaatgaa agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg
    28981 caggtaaaag cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg
    29041 gcgtccagct tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc
    29101 cgggttacca gccgccgaaa gagatgaaag aattcctcga cgaacaccaa aaaatgacca
    29161 gcggtaaata attcgcgtga aacaacagat acaacttcgt cgccgtgcag tcgatgaaac
    29221 ggcagacttg cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggcgt
    29281 gcgcagtgcg caagaactgg aacgcagtgt taaagggatg ctgccctggc agcaactgag
    29341 cggcgtcgaa aaggccgttg agatccttta caacgccttt cgcgaaggaa cgcggattat
    29401 tgtggtcggt gatttcgacg ccgacggtgc caccagcacg gctctaagcg tgctggcgat
    29461 gcgctcgctt ggttgcagca atatcgacta cctggttcca aaccgtttcg aagacggtta
    29521 cggcttaagc ccggaagtgg tcgatcaggc ccatgcccgt ggtgcgcagt taattgtcac
    29581 ggtggataac ggtatttcct cccatgcggg ggttgaacac gctcgctcgt tgggcattcc
    29641 ggttattgtt accgatcacc atttgccggg cgaaacatta cccgcggcgg aagcgatcat
    29701 taaccctaac ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc
    29761 gttttatctg atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg
    29821 cggcatcgca attcctaacc tggcagaact gctggatctg gtagcgctgg ggacagtggc
    29881 ggacgtcgta ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat
    29941 ccgtgccgga aagtgccgtc cggggattaa agcgctgctt gaagtggcaa accgtgatgc
    30001 acaaaaactc gccgccagcg atttaggttt tgcgctgggg ccacgtctca atgctgccgg
    30061 acgactggac gatatgtccg tcggtgtggc gctcttgctg tgcgacaaca tcggcgaagc
    30121 gcgcgtgctg gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg
    30181 aatgcaggtt gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc
    30241 cggcgggctg gcaatgtatc accccgaatg gcatcagggg gttgtcggta ttctggcttc
    30301 ccgtattaaa gagcgttttc accgtccggt tatcgccttt gcgcccgcgg gtgatggtac
    30361 gctgaaaggt tctggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt
    30421 agacacactc taccctggca tgatgctgaa gtttggcggt catgcgatgg cggcgggttt
    30481 gtcgctggaa gcggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga
    30541 gtggctggac ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc
    30601 cgaaatgacc atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt
    30661 cccggagccg ctgtttgacg gtcatttccg gctgctgcaa cagcggctgg tgggcgaacg
    30721 tcatttgaaa gtcatggtcg aaccggtcgg cggcggtccg ctgctggatg gtattgcttt
    30781 taatgtcgat accgccctct ggccggataa cggcgtgcgc gaagtgcaac tggcttacaa
    30841 gctcgatatc aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg
    30901 gccaatttag cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt
    30961 atcaccgcat tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt
    31021 aaataatcgc attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact
    31081 acgacgccaa gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct
    31141 ggaatgaacc cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg
    31201 tcgacaccct cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg
    31261 ctgtagaagc tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg
    31321 aagaaaaact ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg
    31381 actgctacct cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca
    31441 tgcttgagcg tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg
    31501 aagagtcgga aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt
    31561 acgcttacgg ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aagagcccgt
    31621 ttgactccgg cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat ccggaagttg
    31681 atgatgatat tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt
    31741 ccggcgcggg cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc
    31801 cgaccgggat cgtgacccag tgccagaacg accgttccca gcacaagaac aaagatcagg
    31861 ccatgaagca gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga
    31921 aacaggcgat ggaagataac aaatctgaca tcggctgggg cagccagatt cgttcttatg
    31981 tccttgatga ctcccgcatt aaagatctgc gtaccggggt agaaactcgc aacacgcagg
    32041 ccgtgctgga cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag
    32101 gaaccaacat gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg
    32161 aactgaaaac gcgtcgtgag aagctggcga acctgcgcga gcaggggatt gccttcccga
    32221 acgatttccg tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga
    32281 acgaagaact ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgcc
    32341 gtattatggg taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt
    32401 acgttgcccg tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc
    32461 tcggcgacat cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta
    32521 tccactgtac cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc
    32581 acggcttgca ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg
    32641 atgaatcccg taacaccttt aaagtacgct cgcagatcct ctctggtatt cgccagttca
    32701 tggtgaaccg cggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg
    32761 ccgctgcgcg tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta
    32821 tcgcgccgga actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa
    32881 tcaaccgtaa cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga
    32941 tggaactcta catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt
    33001 tccgtactct ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc
    33061 tggacttcgg taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc
    33121 cggaaaccga catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta
    33181 tcggtatcca cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag
    33241 aagtggcaga agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt
    33301 ctccgctggc gcgtcgtaac gacattaacc cggaaatcac agaccgcttt gagttcttca
    33361 tcggtggtcg tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc
    33421 aacgcttcct ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg atgttctacg
    33481 atgaagatta cgttaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg
    33541 gcatcgaccg tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt
    33601 tcccggcgat gcgtccggta aaataagcat tacgttatgc tcacaacccc ggcaaatgtc
    33661 ggggtttttt aattgtgctg cgcgaaagca gacaacaatt ttcttgcttc actattggct
    33721 gcctgcatca ccatccacgg actgaacgcc cacggtgtgg catcaatacc gtgtaataca
    33781 tctgctaaat cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg
    33841 gtggtgcgtg cggcaaatac cggacacact tcattttcca caatgccatt cggatcggtg
    33901 gcgcgatagc gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca
    33961 taacggcaac ggcggatcac cgcgtcttcg ttgctttctc ccagttgtgg gtgcccacaa
    34021 accgagttag tccacacgcc aggccacgct tttttgctca gtgcgcggcg ggtaactaat
    34081 aattgtcctt tggcattaaa cagccaactg gagaacgcga gatgtaagcg ggtgtctgcc
    34141 gtgtgtgcgg catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg
    34201 acgtgttccg tttgcataat ttctcacatg taattctgat cactcatcat tatagataat
    34261 tgaagagtga aatgattaac caagtgagcg tttatcgaca accgcccgtt ttgagcggat
    34321 gccgacaggt aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg
    34381 ccattgcaac acagtcagaa atgacaggaa tagagagagt aaaaagcaac agcagactga
    34441 atataacgcg cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc
    34501 atcagaagcc acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc
    34561 accaggtcag catgatgctg gtagccatta aagaagacat tcagcacaac ggcgctaagg
    34621 gttgcgagca taatgccgct atgcagcaac ggttgcagta cggccggtaa cttagaaaag
    34681 aaatcgtgag agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg
    34741 ttgtaacggt tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc
    34801 atgccgaaca tcaccagccc agcaccgccc agcacaaatt gcggaatgga ggcgaccagc
    34861 accgccattt ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc
    34921 cagcggctat aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacagcaa
    34981 aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct ccggcggccc
    35041 agtcaatacc cacctgaatg atgctaagcc caatagaagt aataaccaca ccggtaacca
    35101 gtggcgggaa taaaggcatc aagcgaccaa taaatggcgc taataatgtg gtgataaaac
    35161 ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc atcccaatgg
    35221 ctatcattgg tgttacagca gcaaaggtca ccgacataat caccggcagg cggatcccca
    35281 taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa aagagatccg
    35341 agctaatgag catcgcaata gcatcttttg agaggcccag tcggtcacca atcataagag
    35401 gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct
    35461 ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg
    35521 gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag agtaaaaagc
    35581 aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag
    35641 gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtgctt aaaccacgaa
    35701 aatgacaaga gaacctgaca atggaattat ctgtaatttt ctcatattga tatttcatta
    35761 atcattctgg catgtcgcac tctagcattt aatcgttttt atctggatag cgctcttttg
    35821 atcggcaatg ttgaaataat gaaagcggca tagattgagg ggcaataatt tgaactcagt
    35881 cgcaaaatta taattaaaac ggtcatgcta cctaatatat gcccgtttat tgcatcgcga
    35941 aatttgagaa gacataactc attaaatgaa agggatgcaa atgaataagt ttatcgctgc
    36001 tgaagctgcg gaatgtatag gctgtcatgc ttgtgaaatt gcctgtgcgg tggcacacaa
    36061 tcaagaaaac tggccgctga gtcacagtga ctttcgaccg cgtatccacg ttgtagggaa
    36121 aggccaggct gcgaatccgg tggcctgcca tcactgcaac aatgcccctt gtgttacggc
    36181 ttgtccggtt aatgctctga ctttccagtc cgatagcgta caactggacg agcaaaaatg
    36241 tattggctgt aaaagatgcg caatcgcttg cccctttggc gtcgttgaga tggtcgatac
    36301 gattgcgcag aaatgcgacc tttgtaacca gcgcagttcc ggcacgcaag cctgtattga
    36361 agtctgccca acacaggcgt tacgactgat ggacgataaa gggttacagt ggataaaggt
    36421 ggctcgccag cgcaaaacgg cagcaggaaa agcgtcatca gacgctcagc catctcgcag
    36481 tgcagcgttg ctccccgtta actcgcgtaa aggcgcagat aaaatttcag cgagtgaacg
    36541 gaaaacccac tttggcgaaa tctattgcgg gctggatcca ctgcaagcga cttatgagag
    36601 tgaccgctgt gtttattgtg ccgaaaaagc taactgcaac tggcattgcc cgctgcataa
    36661 cgctattccg gattacatcc gtctggtaca ggaaggaaag attattgaag cggcagaact
    36721 ttgccaccag accagttcct taccagaaat ctgcggcagg gtatgcccac aggaccgtct
    36781 ttgtgaaggc gcatgtactt tgaaagatca ctctggcgca gtctctatcg gtaatctgga
    36841 acgctacatc accgataccg cgctggcgat gggctggcgt cccgatgtca gcaaagttgt
    36901 tccccgtatc gaaaaagtgg cggtgattgg cgctgggcca gcagggttag ggtgtgctga
    36961 tattctggcg cgcgcgggag ttcaggttga tgtctttgat cgccatccag aaattggcgg
    37021 tatgctgact tttggcattc ctcctttcaa actcgataaa acggtattaa gccagcggcg
    37081 agagatattc accgcaatgg gaatcgactt tcatcttaac tgtgaaattg gccgcgatat
    37141 tacctttagc gatttaactt ctgaatatga tgcagttttc atcggcgtgg ggacttacgg
    37201 gatgatgcga gcagatctgc cgcatgaaga tgcgcccggt gtcattcagg ctctaccgtt
    37261 cctgactgcc catacccgcc agctcatggg attgccggag tctgaagagt atccgctgac
    37321 ggacgtggaa ggtaagcgag tcgtggtatt gggcggtggc gatacgacaa tggattgttt
    37381 gcggacttcc attcgcctca atgctgccag cgtgacctgc gcgtatcgtc gtgatgaagt
    37441 cagtatgccg ggctcgcgca aagaggtggt taatgcgcgc gaggaaggtg tcgagtttca
    37501 gttcaatgtt caaccgcaat atatcgcttg tgacgaagat gggcgtttaa ctgcggtggg
    37561 cctgattcgt accgccatgg gtgagccggg gccggatggt cgccgtcgtc ctcgtccggt
    37621 agcgggttca gagtttgaat tgcccgccga tgttctgatt atggcctttg gtttccaggc
    37681 gcatgccatg ccgtggttgc agggcagcgg aattaaactc gataaatggg gcctgattca
    37741 aaccggtgac gtcgggtatt tacctaccca gacgcatctg aaaaaagtct ttgctggtgg
    37801 tgatgcagtt catggcgcgg atctggttgt cactgcaatg gccgcaggaa ggcaggcggc
    37861 gcgcgatatg ttaactctgt ttgatacgaa ggcatcgtga tgaaatcgtt aattatcgtt
    37921 aatccggctg actgtattgg ctgccgtact tgtgaagtag cctgtgtggt cgctcaccct
    37981 tcagaacagg agttgaatgc cgatgtcttt ttaccccggc tgaaggtaca gcgactggac
    38041 agcatcagtg cgccggtgat gtgccatcag tgtgaaaacg ccccttgtgt tggcgcttgc
    38101 cccgtggggg cgctgacgat gggcgagcag gtggtgcaga ccaattctgc ccgttgtatt
    38161 ggctgtcaga gttgcgtcag tgcgtgcccg tttggaatga tcaccattca gtcgttgccg
    38221 ggggataccc gacaacaaat tgtgaaatgc gatctctgtg aacagcggga agaggggcca
    38281 gcctgtgttg aatcttgccc gacacaggcg ctgcagttgc ttactgaaag agaactcagg
    38341 cgagtccgcc agcagcgtat tgttgccagc ggtgagaatc ctctgtgaaa gaaatctccg
    38401 cgctgaaagg cgcggagaat ctattagatt gcccaaccac ccgcgtaaaa tgcgaccagt
    38461 gcgacaggaa cgctaatttg ttgcccgagt accagactga atgcggtaaa tgccgtcaag
    38521 gaaatcgcac aaccaatcgc cattggcaaa ttggcctaca accccatcag caacgagccg
    38581 aagcccgcga ccagacaggt ggcaacaaac acagctgcgg gaggaaaacc tgctttgccc
    38641 agcattcccg gaacgacgat aacggaataa accatggcca gaaaggtcgt taagccagcc
    38701 agtacttcct gacgaacggt actgccacga gcggtaattt taaaataatt atccagcgcg
    38761 ccctgtggct ttggtgcgtc cggtgtttgt aggatgtctc cagacataat gatgtcctca
    38821 cgaggatgtt aaagttttat tattagttgc gttcgtacac cagacgacca tcaacgtagg
    38881 tgcggtagat cgaacggtca tcgcccaacg tcatcatcac gaacaatttg tcgactaaag
    38941 aaacagagtt gtcatagcgc agctgctgta gcggagtggc ggtgggttcc atcaccacga
    39001 aatcagcctc tttgccaggt aaaaagttgc caatcaaatc gtcaaggccc agagatttcg
    39061 ctccgccgag cgtggccagg taaaacgctt catatgccga gaggcgatag ccttgtaatt
    39121 gcaacacttt gtaggcttcg ttcagcgttt gcagcatgtt gaaagttgtt ccggcaccga
    39181 tatccgttcc catgcccact ttgactttct tctgcccaga tgccgcatgc atcgtgaatt
    39241 gcggtagcaa tcgccggagc tgcgccattt acaccgattt ccgagatcga tttcgccccg
    39301 aacgggccga ctttatcgtc gctcggcacc agcacagcgc ggaaatcacg cggaatgtca
    39361 ccaattttcg gtgcgccgta actgcgtaaa tcacgcgtta acgggtgacc ttcggcgtca
    39421 taaatgatct cttcgctcat actgtggccg atagctcgca gggtagcacc gtagatttgt
    39481 cccaatgcta attccgggtt gaccggtgta ccgcagtcca gcaaggcgta gaatttatcc
    39541 aggcggattt cacccgtacg cgtgttgacg gcaacttcag cgaagtttgc gccatacggg
    39601 aaggcgaaat caggcgtgat ataactgcca gttcccacca gtgaaccaaa gccgatgccg
    39661 gtttcgcctt tatgggcaat atccccgaaa ctaacttcgc ctttcttgcc gcgcacgacg
    39721 cccggcgttg ccagttgaac atctgccact gcctcaccta acatttgcgc gccgtggaac
    39781 agaattttct cccgtagatt ttccgctgcc aaacgcgccg cgttacccga gaagcaagta
    39841 ccggacgagg catatgcgcc tttatcaaac aacgcatgat cggtatcacc ggagataaca
    39901 tgcacgtcct gcggtgggca gtgcagcact tctgctgcca gtttcgtcac tacagtatcc
    39961 agaccagtac caatatccgc accgccagaa tgaacgataa aggtgccgtc cgattccagt
    40021 ttgatcatgc agttagcctg atcgatatcc gggatacccg atttctgcat gataatggcg
    40081 acgccgcgac cgatgtgcca gtcaccattt tgcggtttcg gtgacgacca ttggatcatc
    40141 tcgcgaccct gacgcaggat ctcttccagt gcacagctgg cggcggaagg aacggaggtc
    40201 ggcgctttac cttcaccgat tgcaccgaga attttcagct cttgcccttc gtgtacccgg
    40261 ttacgttcga taatttccag ttggtcgatc tgtaactgtt cagccagttc cgctaatgcc
    40321 atggtgatag cgaagttacc tttcggtgcg ccataaccct gataagcacc atttgggcaa
    40381 atgttgctgt agtaggtggt gacctggaaa tcgacgttat cgcacggata taacggcagc
    40441 gacagcgccg gtccgttaca cggtacggtg agtgagtggt tgccgtaagg gccagtgttg
    40501 gcgcggaaat ccatcttcac tgccgtcagg cgaccatctt ttttcgctcc cagtttgacg
    40561 gtgactttcg cgacgtgacg agaggtgtta gcaataaact cttcttcacg ggtgtagcgg
    40621 aatagtaccg gacgcccggt cacgcaggtt gcccaggcgc acacttcttc cagcaggatg
    40681 tcctgtttgg aaccaaaacc gccgccaact cgctctttaa tgacatgaac tttatgctgt
    40741 ttcatgccca cgaggcgcgc gacctggcgg cgtaagtgcc atggtacctg ggtggaggcg
    40801 tggataacca gacgatcgcc gtccatccgg gtaaagcaga tatgtgtttc agtcgggcac
    40861 tgctgtgcct gcgttgagtt ataggttcgc tcaatgatca catcggcatc ggcaaagcct
    40921 ttgtccatat cgccaatatg accatgaata ctggcggcga tatttttgcg agggcgagaa
    40981 ccgatcggga agttgatgat catatgctcg ccgcgctggg ctgcatggct gttatcgtct
    41041 tccagagtat ctggcgcacc agcaacatac accaccggtt catcgtgcac gacaggcgca
    41101 tcttccgcca ttgcttcgtc gatcgacatt accggcttaa gcacttcata ttcaacgtcg
    41161 atgagcttca gtgcttcgag cgcaatttct tcactttctg cgacgaccgc agcaacacga
    41221 tcgccgacgt gacgcatttt cttgccgaac atacggcggt caagcggtga cggttccggt
    41281 gcgctctgac cccccggggt atagtagata tcagggcaat tcaggtgagt aataacgtga
    41341 acgacgcccg gtaaggcttc agctttgctg acatccagat gagtaatcag tgcgtgagcg
    41401 tgtgggctac gtaacatttt aatgacgcag gcgtcagccg ttacgcagtc ttcaacatag
    41461 cagggtttcg cctgcaccat tttcgcggca tcagttttag gataatgctt gccaatgacg
    41521 tcaaggtcgt cacggaaagt cggagcgata tcaatggtgg cctgcggatt atttttacgt
    41581 gcaaccgcca gttcaatgac ctgatagtat tgttgccagc ctgcatcgcg gctgaacaaa
    41641 ccagaaagcg cgtcgtcgat ctcttcgcgg gtaggtgcgg cgatgcgatc gagaagatcg
    41701 gtgataatca gagctgcagc tggatcgtta taaccagact gcaccacgcc aacatcaacc
    41761 attgcctgtt gaaccagact taactcgttc catttaccca gagattctgc ggtacgaata
    41821 tctgccttct ctaactgtgc ggcaataagc aaggacgcgt taacgatatt accgttaaag
    41881 attattgcgt cagaaccggc aaacccgaag ccatcatcac tgttgcgtac agagtgcatt
    41941 cccatgttaa acaacagctt ttgcacgttt tcgcctggat taacggttag ctcctgaggc
    42001 gcgccattta aagtaaagtg gatgatcata cggcttcctc cccagcctgt tggcagtcgg
    42061 catacaggtc ggctaccaga actcccgtga tatagcgttt ataggccacg ctgccccgca
    42121 aatcttcctg cgggaaaatg gcgttggcga cagcctgttc aagtgcattg ccttccagat
    42181 tttgtttttc gacatcgtgc agacgcagtg ctttactggc gacgccatcc agcgcaattc
    42241 gcataccgtc gtggtctgtc attgcaacgg ctgccgtcac gacggttaaa cctgcctgag
    42301 aacggctaat tttgcgggtc gcacaggtgc gatacggatc tttaatgata atttcggtta
    42361 gcaggcgatc gcatgggcag gccaggtagt cctcgattga cagcgtttcg ccgttgccaa
    42421 aaaccagttc agcatccagt gctagcagga cgggaagcag cactgactct tcctgccggg
    42481 cggcgatttc accaccaatg gtcgactgat tacgaacatg gcgtgagtaa acaaaaccga
    42541 gggcttcacg cagtgctgca ggaataaagc gcgcatcacg cagtggctgc aagcgagaca
    42601 ttgcaccaat ccgcagtgca ccgttatccc agtcaaccca gtccagttcc agatcctgta
    42661 aggaaatggc aatcttttta tcggtacggg ttggtgtagc attgagtttg ctgcccccgg
    42721 cgaaccagac ggcttcatcc tggtagcggc gcttcagttc cagcgcctgt tcgacagagt
    42781 cgggcctgaa aaattgttca atcatcttct ttccttattt aaccctcttc cctgcgggaa
    42841 gagggcatct gtcatttatg ccagcgcatc catccgacgc cacatactgg cagcggcttt
    42901 tctggcttgt gcataaatgg aatcgcaatc gaagttaaac tgacggtctt catagaccat
    42961 cacaccattg accatcacgc tgtgaacgct gcctgagccc ataccgaaag cgatatgccc
    43021 ggcgatattg tctgccagca gcggcgtcgg cgagttgtaa tcgcaaatgg tcaaatcagc
    43081 tttgtaaccg gcttccagaa gcccaaattt cgcgccaaag ttgcggctca tcagttcgtt
    43141 accgttagtc agggctttgg caaaactgtc aggccacagc ggaccacccg catcgcgatg
    43201 tttaaagaag gcaaatttca tctcttcaaa catgtccgaa ccaatgccgt ccgttcccaa
    43261 tgccaggttg cggatgtcgc taaggtgatg gttgtagccg acatggttgt tcatgtttga
    43321 acgggcgtta tgcaccagga acgcatcgcg ctgattgagt agggcgatgt catctttcga
    43381 caagtacagc ccatgagcga ccagcgtttt gctgtcgatg aggtcgaatt gcgccagtcg
    43441 tgccagcagg tctttgccgt accagtggtg actgtaggaa acgtcgtaaa ggtcttccgc
    43501 agcgtgaata tgcaaaccac ggcctgtggc tttcacggct tcacgcagca tctccagacc
    43561 ggcatccggc acggtaaacg gcgcgtgagc accgatatgt gcttccacca gatacggctc
    43621 gcttgtcgct ttcttcgcct catcaatcaa acgggcgaaa cggatgtttt cttctacacc
    43681 ttcctgcaac tctttgatgc cgttgttacg gtcagtagtt tcaaaacagg tcatcgcgcg
    43741 caggccaact tttaaaaatg cgtcgcgcaa tgtggagagc gacccgccga tatacgccgg
    43801 cgaggcatgg tgatcgataa ccgatgtaca tccgctctta atcgcttcca gggaacaaat
    43861 cagtccgctg taatagagcg actcttcatc aagggcgcga tcgagccgcc accagagatt
    43921 tttcagcgtt gagatgaaat ccgggcaagg ggcgatgttt gccataattc cgcgggaaag
    43981 ctccgagtaa aaatggttgt gcgagcagac aattcccggc atcacaatcc gaccatgcat
    44041 ctctttgtag ctggcatcgg ggtagcgttg cgtcagggca tcgccgatag cgacaatcac
    44101 gtcattttcg atagcgatat caacgccttc ctgcactttc gccgggtgta actgcactgc
    44161 ggtgacattc ttcagaatca acatgattac acctccacgc ggcccagcag ataatgatga
    44221 tgctggtgga catggctgat gatgcggcac atatcgttca gctccggtgg tacgttgtta
    44281 aactgacctt cgctgtcgat gtttaacacc cagctttggt tattcagacg tactcgtacc
    44341 cggcaatctt ccacaaggaa gcctggggtg ctgctgttat caaagtcttg cgccaggctg
    44401 aagacggtga ttttgtcttc gtatggttta ccgttccacg gacagaactg agcgcagttg
    44461 ccgcattcgt tacagtaagc gtcgaggtgc agcgtctgga atctattctg gaagccaggg
    44521 actgcaatgg agacgttggc gcggttcggg cagacatcca cacacttgtt gcaaacgtag
    44581 ttacattcga ggcagcgagc ggcttcctgg gcgacaaacg cgtcacgatc gtcactgttc
    44641 accagcgtga tagagatatc gcctttacgt tgatagattt ccgctggatt gacgttgttc
    44701 cagtatttat cgttctggtg ggaacggata ttttcccgac taaggatggc atcggtcgcc
    44761 cgacgcgcgg ttccgacagc agcgacaatg gaggatggtc cgcgctgtac gtcgccgatc
    44821 ataaagacgt cagtcagacg agtttcgcca ttatggtcga cgtctggcca gccgtttttg
    44881 tccagcggca cgcccatcgc attcagggct tcagtatcct gctgttcacc aatggcggtg
    44941 atcaggctgt ctacatgcag tgttactgtt tcgttggttt caaccggacg gcgacgacct
    45001 ttctcatccg gttcgccaag cgacataacg cgcaaggtta aggtgccatc agcatcgaaa
    45061 cgttccggat tattcaggaa acggaactct acgccgtcgt gcaacgcttc ttcatactct
    45121 tcgcgccatg cgggcatctc ttgtagtgaa cgacggtaaa cgatcgttgc tttttctacg
    45181 cctggaacgc gtaacgccgc acgagcgcag tccattgcgg tgttacccgc cccgacaacg
    45241 accacatgtt tgcccagctt gagcgctgta cccttgttgt attcacgcag gaaggggagt
    45301 gatttccaga cattttggtt gtcgcccgcc agtttcacac cgctattttt atcagtgccg
    45361 gtggcaatca gaacatagtg gaagccctga ttttttaact gttcaacggt taaatcgggt
    45421 gagcagccat actcaaattt cacgccgtga gcggcaacaa aatcgatatc gtgctgaatt
    45481 aactctgcag gaatacggaa ctgaggaatg atatttttca ccacgccgcc agcattggct
    45541 tcgcgttcaa acagcgtaac cggatggccc gctctggcaa ggaagtaacc tgctgccaga
    45601 cccgccggac ctgcaccaat cacggcaacc ggatggcgtg aaccagaacc ggcgggtttg
    45661 tgccagcgtt gcttatattc atcccaacct ttttccagcg cgactttttt cagttcgcgg
    45721 atattcagcg cactgtcgta atccaggcgg gtacagttgt attggcactg gtgatcgcaa
    45781 atatgaccgg taatggcagg tagggcgttg cgttggtaga tgagttccag cgcgtcggca
    45841 tagcggtgtt cgccaagcag acggatgtat tccggaatat cttgcttgat agcgcaggca
    45901 gtaacacagg gggcaacgta gcaatcggtc agcggcaggt cttctgccac ttcaatacgc
    45961 tcttctggct tccagtgttt ctgggtgtat tccatggtta acgcgtctgc tgccagtctg
    46021 ttcagtcgtt cgacgtcaac atggtcaagt ccccaggcgt cggagccttc cagctcgcgc
    46081 atgcaggcac ttaagcgcag atagccgcca ggtttcagca ggtcggttgc catagtaata
    46141 gggcgaatac ctgtatcaaa aatatcgcgg atagtcagct gactggcacc accggaataa
    46201 gaaatgggca gtttgccgtc aaaggcgcga gagagaactg ctgcaacatt gatggagagc
    46261 gggaacagcg cacggcctga catatacatc tcttcaccag gcagtgcgcc tttattgttg
    46321 atggtgccga gagtgttagt cagttttacg ccaaagccga gtgatttttc ttttgccagt
    46381 accatcaggc gctccagcat ttccagtgct tgcgtcagct tgaggtcgtg atcaaatgac
    46441 tcttctttta agcctatgta accgaaaccg cagacatcga ggatctcacg aacacgcgcg
    46501 taccccagta aggtcgggtt aagtttgaca aaggtgttga gccctttttc ttccagcatg
    46561 tagcggcaaa tggcttcgat ttcatgcgga ggacagccgt gcatggtgga gagggtgacg
    46621 ccctgcacca tactggtggg gatgcgagcg ggtaaggctt gcaagttttc gcgtttttcc
    46681 tgcaatccgt ggcgggctaa aaatgcgtca tcctggagta atttattcag cgtatcgcga
    46741 tattgagcga atttcggatg gtcagatgcg tccatcatat tgtcgatgaa ctgttgcatc
    46801 ggcggttgct taataccttc gaggttgtaa ccgacgctca tattaaagat gaacgattta
    46861 ccggaatcag aaggctgtaa catcgcttcg agaaggtgca gggcaaacca ggctttgagg
    46921 tattcatccc aggctttaag caaggtaaat tcggtagacc attcggtgtt aaagcattcg
    46981 tcttcggcat caatacaggg cttttccagc tccaggcggt caagaatttg gacggttttt
    47041 agttcgatga agcgtccgcc agtcagccag gaagtgacaa tattttgcgc gagctgcgtg
    47101 tgcgggccag cggcagggcc gacgggagtg gcacaggttt caccgaagac gctaacagtt
    47161 ttacctttta caggtgagta aaactgttgc tcgggaatac caaagattga gcgttgttgt
    47221 tggtattcat caaatatgcg cgtcaaaagt tcctcaaacg gaatgggacg cataatatcc
    47281 cccataaccc tctccttagc ttatacagat atcattgagt gaccggtgtt gagaaatccg
    47341 gtggtttagg gggatagagc aacaatcgca ccactcttgc caggtttggc gggaaatata
    47401 aaaaaatgtg aggtggctcg aaacttaaag gctttttgtg aatgaactca caggtgccaa
    47461 atgagaatta aaatcactcc tgaaaattat tattgtcagc ccatttatca ctttttctct
    47521 tttctcaaaa tcagtgattc cttatcatta tgataaagag tgctggagag attaaatatt
    47581 tgaacgcaat cgtttaacta cattttctaa attttagtat accttttttg cgctgtaata
    47641 aagtcgtccg gagtatcaat atctaaaatt atttctgcat tttctatttc gacggaataa
    47701 tgatctccca taaggagtgc ctgacgcata ttggtgacat tgggtcgctg gattgcctgc
    47761 atcaggcatg gttttgacac taaaatcgga tggccgggga tgccattatg gagtggcagt
    47821 attgcgccat catttcgtaa cgaccagatt tttctaaaaa tatcgatggt gagggttggc
    47881 atgtcaccgt gggtgagaaa acaatgttct gtttgcactg cgggtactgc ggccttgacg
    47941 gatgttagta aaccctgcgc ataatctggg ttgtgaataa tagtgatatt gctctggttc
    48001 gcataacgtt cgtgcagttc attaccacga tagccggtga ctaaaataat tcggctacaa
    48061 aactgcaacg catttttgat acttgtatca agaattgttc cctgttccca ggggaacatc
    48121 attttccatt gccccattct tgatgataat ccggcagcgg taattataca gtcgatggct
    48181 gacatagtgg ttccattatt tataggggct aataacgcct gataattgcg ttacttcctg
    48241 atggattaag ttggggataa tttatcccag agaggtcata aagactcgtg aaaagtatag
    48301 ttgacccatc ggcattagtc attgatttag gtgcgcagaa acgtcccact gtgatttctg
    48361 ttgttggtgc tggggggaaa accagcttgc ttttttggct ggcagaactg ctccaggcta
    48421 gcggcaggcg tgtgttaatc acgacgacga cacatatgtt tatgccaacg tctcactggc
    48481 ccgtggtttt ctgtcgtgat cccgccatgc ttcctcatgc gtctcttaca tcccccattt
    48541 cattttgttt tcacagctgg aaagcgaacc agggaaaagt gcagggattt acgccagaag
    48601 cgattgatgc actggtgcaa cgaccagagt gtgacgtaat tctcattgaa gcagatggct
    48661 cgcgtggaat gccgttaaaa gcgcctgatg agcacgaacc ttgcataccc aaaagcagtt
    48721 gctgcgtgat tgctgtgatg ggaggacata ctttgggcgc gaaagtgagc acagaaaatg
    48781 tccatcgctg gtcgcagttt gctgatatta ccgggttaac acctgatgca accttgcaac
    48841 tgagcgatct cgttgcgctg gttcgccacc ctcagggggc gtttaaaaac gtaccgcaag
    48901 gttgtcggcg agtctggttc attaaccgtt tttctcaatg tgagaatgcg attgcacaaa
    48961 gcgagctcct tcaaccgctg caacaacacg acgtagaggc aatctggctg ggcgatatac
    49021 aagaacatcc tgcaatcgcg cgcagatttg tgaattagcg gcccgggtat tccgggttac
    49081 tgaaaggata tccattgagg ttggtatgaa tattttcaca gaggctgcaa aactcgaaga
    49141 gcaaaattgt ccgtttgcga tggcgcaaat tgttgatagc cgaggctcga ctccccgcca
    49201 ttctgcacaa atgttagtgc gcgccgatgg ttctatcgtc ggtacaattg gtggcggaat
    49261 ggttgagtgg aaggtgattg aagagtcact tcaggcattg caggaacgta agccgcgatt
    49321 attccatgga cgtatggctc gtaacggtgc ggatgctgtc gggtcagatt gtggaggtgc
    49381 aatgtcagta tttatcagcg tccatggtat gcgtccacgt ctggtgttga ttggcgcggg
    49441 gcatgtcaac cgggcgatag cccagagtgc ggcgctatta ggatttgata ttgccgttgc
    49501 cgatatttat cgcgaaagcc tcaatcctga actattccca ccatcaacca cgcttctcca
    49561 tgctgagtcg tttggtgcgg cagtggaaga actggatatt cgccctgata attttgtcct
    49621 gattgccacg aataatcagg atcgtgaagc cctcgacaaa ctcattgaac agcccattgc
    49681 atggttgggg ttgctggcaa gtcgccgcaa ggttcagctt ttcctgcgtc aattgcgtga
    49741 gaaaggcgtg gctgaagaac atattgcccg tttacatgcg cccgttggtt acaacatagg
    49801 tgcggaaacg ccgcaggaga tcgccatcag cgtgctggca gaaatattac gggtgaaaaa
    49861 taacgcgccg ggtgggctga tgatgaaacc ttctcatcct tccggacacc agctggtggt
    49921 gattcgcggt gcgggagata tcgccagtgg tgtggcgcta cgtctgtatc atgcgggttt
    49981 taaagtgatc atgctggaag tggaaaaacc gacagtgatt cgttgtaccg tggcgtttgc
    50041 ccaggccgtg ttcgatggcg aaatgacggt cgaaggcgtc actgctcgcc tggcaaccag
    50101 ctctgcggaa gcgatgaaac ttaccgaacg cggattcatc cctgtgatgg tagatcccgc
    50161 ctgttcattg cttgatgaac tgaaaccgct ttgcgtggtg gacgctattc tggcgaaaca
    50221 gaatttggga acgcgggcag atatggcacc agtaacaatc gcgcttgggc cgggctttac
    50281 tgcagggaag gattgtcatg cggtaattga aacaaatcgc gggcactggc tcggtcaggt
    50341 gatttactct ggttgtgcgc aggagaatac cgatgttcct ggcaatatta tggggcatac
    50401 cacccgacgg gtgatccgtg ctcctgctgc aggcattatg cgatccaacg tgaaattagg
    50461 cgatctggtg aaagagggcg atgtgattgc ctggattggt gagcatgaaa ttaaagcacc
    50521 gttgacgggg atggtgcgtg gcttgttgaa cgacggcctg gcagtggttg gtggttttaa
    50581 aattggtgat atcgatcctc gtggtgaaac cgctgatttc accagcgttt ctgataaagc
    50641 ccgggcgatt ggcggcggcg tacttgaggc gttaatgatg ttgatgcatc agggcgtgaa
    50701 agcgacaaaa gaagtgctgg aagtggctta aatgaaaatg gccggatggt attacccacc
    50761 cggccttaaa attactgaca aatcaccgtt ccggttttgc cttcaatccc ttctttcgct
    50821 ttgctcaata cggtaatcag tgcttcacga cctgcgcgag agcgagcaaa cgaggcggca
    50881 gcttccactt taggcagcat agaacctttc gcaaaatgac cttcttcgat aaagcgttca
    50941 gcatcactta atgacaggcg atcgagccac tgttcattct ctttaccgaa gttaatggcc
    51001 actttttcta ccgcagtcag aatgatcagc atatcggcat caatcatttc tgctaaacgg
    51061 gcgctggccc agtctttatc gataaccgcg ctggcaccac gcagatggtt accttcacga
    51121 ataacaggga taccaccacc gccaacggcg atcaccacct ggcctgcatc taccagagct
    51181 ttaaccgttt ctttttcaat gatatcaacc ggttttggcg aggcaacaac acggcgatag
    51241 ccgcgaccag catcttcttt cagagtgtaa ccctgttttg tcagttgctc tgcttcttgc
    51301 tcggtaaaga acgaaccgat cggcttggtc gggttgagga atgccggatc gttagcgtcg
    51361 acttcgacct gagtaaccag cgttgctaca ggtttattaa tgccccgaga aagcagttct
    51421 tctcttaacg cgttttgtag atcgtaacca atataaccct ggcttaaggc aacacagaca
    51481 gacatcggca gcattggcga gtgtgcttca gtttttgcgg cagcttcaaa ggcttggtta
    51541 atcatgccca cctgcggccc gttaccatga gtgacgataa cttcatgtcc ttgggcaatt
    51601 aaatcaacaa ttgcctgaga agtaattttt accgctttca tttgtccggc caggtcatcg
    51661 cccagcgcat tcccgcccag ggcgagaaca attttcttac tcatttttcc tcactcatca
    51721 ttcttgattg tgtatagatc cctgacagtg gttagagcac gggagggaca aacggtttgc
    51781 gtcgcaggaa acgacctcgc ccggctttgc ctgtaaaggt gccgtcacag aaaatcgttt
    51841 caccacggga taaggttctg acaatcgcgc cctgacaggt aaaaccctcc catggcgagt
    51901 agtcggcgtt gtcgtggaga tggcgatgct gaatttgttg gctctgacgt gggtcgataa
    51961 tcaccacgtc gccatcggaa ccgggcgcta ataatccttt ttgcggccac aggccaaaca
    52021 gcctggcggg cattgcgctg gttaattcaa caaagcgttc cggtgttata cgtcccgtca
    52081 tcacgccact ggaaaacagt aactgcatgc ggttttccac accgggtaag ccatttgggc
    52141 agcgactgaa atcgccttta gaaatttgca ggcgttgagc catcgagaag gtgcagtgat
    52201 cggtcgccac cacgtcaatc gcaccatcgc tgatgccaca ccacagtttg tcctgctcgc
    52261 gtacgttacg cagcggtggg ctaagaatga acttcatgcc atcttctgta tcgtaactgc
    52321 gttcgtccaa caggagatat tgtgggcagg tttcaaccca gactggctgg tgattcgcac
    52381 gggcaagacg cagataatcc agacctaagc cgttagacag gtgcacgata tagagcgggg
    52441 cgttaccggc aatttgtgcc aggttaatca tgcgggcgat ggcttccgct tcgcattcca
    52501 gagggcgact caaggcatga tagcgcggcg cggttaaccc tgcggcgata aattccgccc
    52561 gcttgctggc gatagccgca tcattttccg ggtgcacggt ggtcagcgcg ccggattcat
    52621 gcaagcggcg taatgcctgc aaaacctcgt catcgttgag tttgtattga taggttaaat
    52681 agagtttaaa actgctcagt ccttcctcga ccatcatcgg gatttcgtcg aggattgcgt
    52741 gattaatgtg ctggatcaca ccgtgaaagc tgtaatcgat gaccgcttta tgggcggcat
    52801 aaccacgata aacctccagt tgatggcgta accgacagcc gtttgggcca aatcccatat
    52861 ggtcaataat ggttgttgta ccgccacacg cagctgcgcg ggtaccggta aaaaaatcat
    52921 cacaactgcg cgcgatgccg acatcaatat tgaaatgcgt atggacatcc acgccgccag
    52981 ggaaaacgta acagccagtg gcatcaattt cttcatacgg gagctgcggc gaaatattgt
    53041 tgcccaactg gcgaacaatc ccgctttcaa tcagcaaatc ctgtttggct tgtccatctg
    53101 cgttaacgac agtgccgttt ttgatcaata cgcgcatagc aaactccaaa aaccctccgg
    53161 cgaaccggag gggaataagt gaagagtaat tactctgttg ccagccagct taacgggatt
    53221 gcagcgtaca tcgcggcaca ggtcaccagg tgagatttcc aggttttttc gttaggtgca
    53281 tgcgcttcag gttctttacc cgggccaaag ccgatcaccg gaatgccgtg acggcccatg
    53341 atagatacgc cgttagttga gaaggtccac ttatcaacaa ccggcgcttt gccaaacagc
    53401 ccttcgtagg cattcaccag tgctttaacg gtgaagtgat cttcttccac tttccaggtc
    53461 gggaagtagc attcggttgg gtaaaccagg ccagtccagg acggacggtc gtagttgtac
    53521 atagaaacaa ccgcgttagc tttctgtact gcaggcaggg cgcggatttc gtccagcgcg
    53581 ccttcccagg tttcgcccca ggtcagacgg cggtcaatag agacggcgca gctatctgct
    53641 acagcgcaac ggcttgggga tgtgaagaag atttcagaaa cggtgagggt gcctttgccg
    53701 aggaattcgt cataacccag acgttgggag agttcttgta attcgccaag aatcggaccc
    53761 attttgaaaa tggcgttgtc accgcgttct ggcgcagaac cgtggcagct aacaccctga
    53821 acatcaatac gaatttccat acgaccgcgc tgaccacggt atatctggca gtcggttggt
    53881 tcggtactga ccacaaattc cgggcgaatg ccggattgtt caataatgta ctgccagcac
    53941 agaccgtcgc agtcttcttc ctgcacagta ccggtaacca gcagggtata ttcatcttcc
    54001 agaccgaggt ctttaatgat tttaccggca taaaccatag atgccatgcc gccttcctgg
    54061 tcggaagtac cgcgaccacc gattagctca tcagtttcca tgccttcgta cggatcgaag
    54121 tcccagtttt tgatgttgcc aatgccgacg gtatcgatat gagcgtccat tgccaccaga
    54181 cgcgggccgt ggccgatata accgagaacg ttgcccatcg ggtcgatttc aactttatcg
    54241 aagccgactt tttccatctc ttctttaata cgatgtacta cgcgtttttc gtcgcagctt
    54301 tcactgggaa tagcaaccat gtcgcgcagg aagcgagtca tgtccgcttg gtaatctttt
    54361 gctttttcaa gaatcagttt gaatggaata ttcttagcca ttatttttct ccagattttt
    54421 tactccccgt tttgcaggga gtagttccaa tagggtgatt aaggtgctac agcgtgtttg
    54481 ccttcccaga caacttcgcg gtagtgcttc acgtcggtgt cgccttcagt gctgataacc
    54541 agcactacgg catctttgtc cagcgccagt ttttccatca ggctttgatg ttgcgggtga
    54601 taatgaaccg ctgcgagaac gcccaaaccg acagcgccgg attcaccgga gatgatgcgc
    54661 gggtcgttgc cgtacggatt acccagcacg cgcataccta atgcggcaac gctgtcctgg
    54721 caggagatga attgggtggc gcagttacgt aggatttccc agcccagcgg gttaggttcg
    54781 ccacaggcca ggcccgccat gatggtggcc atatcaccgc caacgttgac gatgtcgcct
    54841 ttgacgccgg agcgataaat acagtcagct ttgtcaggtt caacaataat gctgtgcaga
    54901 ttttgcgggc tgtagacgtc gaccagataa cccagcacac caccggccat tgctccgaca
    54961 ccggcttgca gcagaacgtg cgtcggggtt acgcccattt cacgcatttg ctcgacggct
    55021 tcatctgcca gggttgcgta gccttgcatg atccaggttg ggattttggt gtaaccttcc
    55081 catgccgtgt cctgtaccac ttcccagccg tgctgctgcg cgtgttgcat ggtcaggcga
    55141 acggtatcgt catagttcat atccgtgacg atgcactcgg caccgaggtt cagaatggcg
    55201 tcaacgcgtt cctgagcaga acctttcggc atgtaaatca ccgcattctg tccgagttgc
    55261 tgtgctgccc acgccacacc gcgcccgtgg ttgccgtcgg tggtggtcgc gaaagtcatt
    55321 ttttcgccga tggcattttt caggtgctca aatgacagcg tttcgatatc aagatgatat
    55381 ttttcacaca ataattgagc gatggcgtac gcaccgccaa gcattttgaa ggcgttcaga
    55441 ccgaatcgtt ttgattcgtc tttgacgaga attttcttca caccaaaaag gtttgcgaga
    55501 tcgtccagcg cacaaagcgg tgttgggcga taaccagcta ttttctggtg gaactggcgc
    55561 gccagtttgg cctggctttg cgaaaagagc ggtgatgttt cgccgttgaa aaatttgtta
    55621 tcggcgatat caatcttcaa tgagaaaacg gacatatcct atccttttgg cctctggaaa
    55681 tagagggccg accagcagat cggcccgagg ggttatttca cgcgttcttg cgcttctttc
    55741 agcagttgct cgagcagtgc acctggtttg gcgtatttac gggacaggat catcgcggcg
    55801 atgatataag gcttccagct ggcttctttg taggtagcga tacggtattt ttcgaatacg
    55861 ccttcagtca cttcaccttc tttgcaggat acgccgctga tatcagctgg caggcagtgc
    55921 atgtacaggg cttcgccatc acgggtcagt tccatcatct cttcagtaca atgccagtct
    55981 ttgtgttgcg cgttctgtgc cagacactgt ttttccagtg cttttaagcc ttcgtggtcg
    56041 ttcgcacgca gcaattcagt acgctcttcc atcactttgt aaggtgccca tgacttcgga
    56101 taaacgatgt ctgcgtcttt gaaggcttct tccatgctgg tgacctgacg gaagctacca
    56161 ccggaggctt tagcattgtt tttcgccact tcaaccacat ccgggatcag gtcgtagcct
    56221 tccggatggg ccaaggtgac atccataccg aagcgagtca tcagaccgat gatgccttgt
    56281 ggtacagaga gcggtttgcc atagcttgga gagtaggccc aggtcatggc gattttttta
    56341 cctttcaggt tttccagtga accaaagtgt tcacgtaacc acgcgaggtc agccattgac
    56401 tgagtcgggt ggtcaatatc gcattgcagg ttcactaaag ccggacgctg tggcagtaca
    56461 ccctgcttgt aaccgtcatc aagtgcagcg ccaacttcac gcatataggc gttgcctgcg
    56521 cccagataca tatcgtcgcg aataccaata gcgtcggcgc agaaggagat catattggcg
    56581 gtttcacgca cggtttcgcc gtgagcgatt tgtgatttgc cttcgtcgag atcttgttgt
    56641 gcaaggccga gcaggttaag cgcggaagca taagagaagc gggtacgggt ggagttgtcg
    56701 cggaatacgg aaatacctaa tccactatta aagactttgg ttgagatgtt ttcagcacgc
    56761 agtgctttta atgctgcggc aacgtccagt acttgtttca gttcatctgg cgtctgttcc
    56821 cacgttaaca aaaaatcttt ctcgtgaagg tgagaggtca gcgaattgat atccttaatc
    56881 agctcattaa cagttttcat catacagtcc cggtagtgga atgacaggca aagtggttta
    56941 tttcactgtg acttgccaaa gtgattggtg ttttcatagc tatacctggg actaacaata
    57001 agtgtgccag aattatccgc gcggctgcaa aagactaatt tgacggataa tgtgtggggt
    57061 tgttcacaaa ttgatgccag cttctggttg tttgtaaaat acgaaaacgt ttggcttttt
    57121 ttatcgcgtt atttatttgc tgctaagtat cagaaataag ataaaggggc aggctatctc
    57181 tcttcagttt gataaactca ccgccgtaat tctcatattg atatagaaat gtgatatctc
    57241 ttccataatt gcattagaaa atgataaaaa tcttatgttt ttatcaaagc caatttcgcc
    57301 tgagttaata tcatcatgat ctgtgaacca tcaacgtctt caatgatcga cttttaagta
    57361 agtctggaag gtaaagctat ggagcttgct actacgcagt cagtattgat gcaaattcaa
    57421 ccgacaattc agcgttttgc cagaatgctt gccagcgttt tgcagtttga ggttgagatc
    57481 gttgatgaaa acttgtgtcg cgtcgccgga acgggcgcgt atgggaagtt tcttggtcgc
    57541 cagttgagcg gcaactcacg cctgctccgc cacgtcctgg aaacgaaaac tgaaaaagtt
    57601 gtgacacagt ctcgcttcga tcccctttgc gaaggttgcg atagtaaaga aaattgccgc
    57661 gaaaaagcat ttctgggtac gcctgtcatt ttacaggatc gttgtgttgg ggtgataagt
    57721 ttgattgccg ttacccacga gcaacaagag catatcagtg ataatttacg cgaattttct
    57781 gattatgttc gccatatatc caccattttt gtttcgaaac ttctggagga tcaggggcca
    57841 ggagataaca tcagtaaaat attcgcgacc atgatcgata atatggatca gggcgtatta
    57901 gttgttgatg atgaaagtcg ggttcagttt gttaatcaga ctgccttaaa aacacttggt
    57961 gttgtacaaa ataatattat tgggaaacct atccgtttca gaccattaac atttgagagt
    58021 aattttactc atggacatat gcagcatatt gtttcgtggg acgataaaag tgaattaatc
    58081 attggtcaat tgcataacat tcagggccga caattatttt taatggcatt tcaccaatcg
    58141 cataccagtt tttctgtagc aaatgcacct gatgaaccac atattgaaca attggttggc
    58201 gagtgccgtg ttatgcggca attaaaacga ctcattagcc gtattgcacc cagcccatcc
    58261 agcgttatgg tggttggtga aagcggcacg ggtaaagaag tcgtcgcccg agcaatccat
    58321 aagttgagcg gaagacggaa taaacccttt attgctatca actgtgccgc gattccggag
    58381 cagcttctgg aaagcgaact gttcggttat gttaaaggcg catttactag tgcttctgcc
    58441 aacggtaaaa cagggttgat tcaggcggcg aatacgggca cgctgtttct cgatgaaata
    58501 ggtgatatgc cattaatgtt gcaggctaaa ttactgcgcg ctattgaggc gcgtgaaatt
    58561 ctgccgattg gtgccagtag cccaatacaa gtcgacattc gcatcatttc tgcaactaat
    58621 cagaatttgg cccagttcat tgccgaaggt aaattccgcg aagatctctt ctaccgactt
    58681 aatgttatcc cgataactct gccaccgctg cgtgaacgtc aggaagatat tgaactattg
    58741 gtgcattact ttttacatct gcatacccgt cgtctgggat cggtttatcc tggcattgct
    58801 cccgatgtcg tcgaaatatt gcgtaagcat cgttggcccg gaaacctgcg cgagttaagc
    58861 aatttgatgg aatatctggt taacgtggtt ccttcaggtg aagttatcga cagcacgcta
    58921 ttgccgccaa atctgctgaa taatggcaca acggagcaaa gtgatgtaac agaggtcagt
    58981 gaggcgcacc tgtcactcga tgatgcgggc ggcacggcgc tggaggagat ggaaaagcaa
    59041 atgatccgcg aggcgctttc acgtcataac agcaagaagc aagttgctga tgaactgggc
    59101 atcggcattg ctacgctcta tcgcaagatt aagaaatatg agttgttaaa cacataacct
    59161 cagtgaatcc ggcctgaatt caggccggat atccttttac ttcgttttct cgcaatccag
    59221 aactgtattt acaatcatct gataccccgt gcagcgacaa agatttcccg ccagtccgcg
    59281 acgaatttcc gtaatggtta atggcttctc gcgtggtttc gccagcattg ccgtggtagc
    59341 cataatcagg ccaggcgtac aaaacccgca ctgcactgcg ccggatttcg cataagcctg
    59401 ctgaacatga gaaagttttc cgcctttcgc ttcaccttcc agcgtgcgga tctcctttcc
    59461 ttcagcccag gcggcaaggt ataagcaact gtctattgct gtgccgtcga ccaacaccgt
    59521 acaggcacca cattcaccca cgcagcaccc ttgtttgaca cttagcagtc cttgttcgcg
    59581 gagtaattcc gagagcggcg tgcctggtgc ggcgtgaagc tgaaaaggca tcccgttaat
    59641 ggtgcattcg atggtaattg tttcgctgtg attcattgca attttccccc cgccgcggcg
    59701 acggcttcgc taatcacttt tttggtcatc gtctggatga gatgcagacg aaactcttta
    59761 ctggcccgcc atgaagaacg cggggcgaca tcttgcagga cagattcgct gatagcttcc
    59821 agcgtttgca ggtttaatgg cgcattttgt gcagtctgtt cggcatgttg gcagcgaatc
    59881 ggcgttggcg cggcaacacc aaatgccagg cgtaattcgc tgaaattgcc gttatccagt
    59941 cggcaatgtg cggcgcagcc aatcgttgaa atatccattg cgtcgcgcat ggcatattta
    60001 aaatgcgcgc tgcccgcgtg ttctttcggc tgtggcggaa aatgaaaggc gacgaggatt
    60061 tcgtcatgct caagagacac tttgcccggc ccggtgtgaa agccattaat cgggacgaaa
    60121 cgaacaccgc gcggggagtg gatctccagt ttcgcgtcat aaattagcgt tggcgtggca
    60181 gaatctgcgc tggtggcacc gttgcaaata tttccaccgt aggtagcgac gttacggatc
    60241 tgcggtccag caatggacgt ggccgcagca cataacgccg ggagatgacg ttgagttata
    60301 ggagcttcta ttagctgggt aaatgtcgtt gcagagccga tacgtagcga gccatcttcc
    60361 gccagcgtaa ttccccgcag ctccgccaga ttatgaatat caacaatatg gcgataacgg
    60421 tcattgtgat ggtggagctg aatcagtacg tcagtgccat cggcgagcag tttggcctgc
    60481 gggttgtcag ccagcaggtt gatggcatcg gcaagggttg ctgcgcgatg gtaagaagca
    60541 aaatcaaaca tgatgttatc ctcaaatcaa tcctgccaga tggaactctt catataaccg
    60601 ttttggcgtc agcggcagtg tattgattgc aacaccggta gccatcttca ccgcgttacg
    60661 aatagcagcg gcaacaggaa ttattggtgg ctcacccagt gacttatgtc cgtatgcgga
    60721 ttgcggctca ttgatttcga cgaacgcgct ttccagttgt ggcagatccg gcatggtcgg
    60781 cattttgtaa tccagcagat tggggttacg gaccacgccg cttttagcat cgatgatcat
    60841 ctcttcaaat agcgcccagc caatgcccat tcccattccg ccgtgtacct gaccttctgc
    60901 cagcagtgga ttaagaatat gccctgaatc atgaacgttg aggatgcggt tgatggtgac
    60961 tttgcacagc gcaatatcga ccgtcagatc aacaaaggta cagccaaacg ccgatgggtt
    61021 agtggtggtt ttgatggagc tttcagcaga gagctgcccg ccgcgttcag ggtggtagaa
    61081 agcgtccatc gccaaatctt ttaacgacat taacggctct tccggtcgtt caaccagcac
    61141 gatatggcct tttatcaggg tcagattcat cgctgactga tgtagcatga ctgcggcgtg
    61201 agcgatgatt ttctctttta ataatagtgc cgcactgcgc agcgcaggcg cggcaacata
    61261 gctctggcgt gaggcaaatg cgccgggagc gaacggcgta acgtcggtat cttgagttga
    61321 aataacgcga acgtcgctga ccggaacccc cacggtttct gccaccattt gcgagaagac
    61381 ggtgtcggca ccctgaccga tttccgtcgc gccgctttgc acgttgatgg ttccatcctg
    61441 attcatcaga aggcgcgcgc ctgctatttc tacgccgaca ggccaggtgt tagaggtata
    61501 gctaaaacag gcgacgccaa cgccgcggcg caaattgcct tgctggttct ggcattctgc
    61561 acggcgtttt tcccattcaa agattttccg gcctttttca agacactccg gcaaccctgc
    61621 gctgtaaata cgtttgcccg tgagcggatt agcatctcct tcgcgggcgg cgttgcgtaa
    61681 acgaatttca acaggatcaa tacctaacgc tgtcgcggcg tcatcaagca tagactcaac
    61741 ggcaaatacg acttgtggcg cgccataacc acgcatcgca ccagccgagg ggaggttggt
    61801 atagcaggtc tttgaactgt aagcgtaggc acaacgagga taaaggtaag cgactttatt
    61861 cctccccgca gaagcgatgg agtgcccgtg agatgcataa gcgccggtgt tagacagaac
    61921 atccagacta taacctttca atgttccgtc gcggttcacg cccatttgcc cgtcaatggt
    61981 aaaagcgtgg cgggtacggg ttgcgaggaa acactcttca cggctaaggg aaactttcac
    62041 cggaatgccg ccaagcttgc tggtcaggaa tgctgccatt ggctcttcca gtacatcctg
    62101 tttattacca aaaccgccac cgacaaacgg tttgatgact cgtacgcatg accagggaat
    62161 atccagcgcc tgaccaacca cgcggcgaac aatgtgcggg atctgggtgc tggaaacgat
    62221 ggtaattcgc gagtcatcct ccatccacgc cagcgatgtt acgctttcca tgtgacaatg
    62281 ttgaataacg ggggtctgat agtgcccctg tacctggtag tcggcggcat cgattgtttg
    62341 ttggacatta cccgtcgaca tcgtgctttg tttcagtaaa ttgccaccgt tatggattgg
    62401 tgcagcgtct tctgccagcg ccgcttctgg cgtggtgata acgggtaatt cttgccactc
    62461 aatgctgacc aattgcgccg ctttttctgc cgtgagttca tcgcgggcca cgacgatggc
    62521 aacggcgtca ccatgatgac gaacatggcg agttagcagt gcgcgatcgg cggtatcgcg
    62581 cttgttttcg tcaagtgtcc aggcatgccc agctgtagcg aatggaatat caggcacatc
    62641 ttcccaggta aaaatcgcca gtacgcccgg caaacttctg gcttgttcat cattaatact
    62701 tacggcataa ccatgtgcga tagggctacg tacatatttc gcataacaca tgcccgccat
    62761 aacataatcg tcagtatatc gtgcccgccc ggtgacctta gcaatggcat cgacgcgcat
    62821 gcatgattca cccgtagcgg ttgcttcccg cgcttccata gaaatcccct cgatttgaac
    62881 atttggttct tatggcaaag atttagtcta agcaggcaag atttacgcca gaaacgacga
    62941 taagcgttag gggaggtagg aagtgtgatg acgttaaaaa aacacagata tgatacgaaa
    63001 caggactctg aataggggca gtgatacgta tcaatatgag tggcgataaa atcaatgtga
    63061 taaattggga ttattacgca tcaggatggg aaatagggta aaaagtcagg aagttcgata
    63121 cctctacagc gaatattttg ctgatgatac ttccctttgc caaatgaaat taacctcttt
    63181 ctctattacc acgtttttcc agaagcaaga gattgggtat caagaggctg gctgctatga
    63241 taaggcgtct tgttttttaa gcgaggaaag attttgagtg cgggacgcct gaataaaaaa
    63301 tctctgggta tcgtgatgtt gttatcggtt ggactgcttt tggcgggctg ttcgggtagc
    63361 aaatcatccg atacaggaac gtattccggc tccgtttaca ccgtgaaacg gggggatacg
    63421 ctatatcgta tttcgcgcac cacgggaacc agcgtaaaag aactggcgcg actgaacggc
    63481 atttcccccc cttacaccat tgaagttggt cagaaactaa aactgggtgg ggcgaaaagt
    63541 agcagtagta cacgtaaatc aaccgccaaa tcaacgacca aaaccgcatc ggttacaccg
    63601 tcatcagcgg taccgaaatc atcctggccg ccagtagggc aacgttgttg gttatggcca
    63661 acgacaggga aagttatcat gccgtattcg acagcagatg gcggcaataa agggattgat
    63721 atctcagctc cacggggtac acctatttac gccgcgggtg caggaaaggt ggtgtatgtg
    63781 ggcaaccagc tgcgtggcta cggtaatctc atcatgatta aacacagtga agattacatt
    63841 acggcttacg cccataatga cacgatgctg gtaaataatg ggcaaagcgt gaaggctggg
    63901 caaaaaatcg ccactatggg gagcacggat gcggcatctg ttcgcctgca tttccagatt
    63961 cgttaccgtg caacggcaat tgatccgcta cgttacttgc cgccgcaggg cagcaagcca
    64021 aaatgctgat ggcgaattaa tcagcagtca gcagcgcggc acttgctaag gagagcgtaa
    64081 ggtttataat gccttacgca tctcgaagcg ggcgtagttc aatggtagaa cgagagcttc
    64141 ccaagctcta tacgagggtt cgattccctt cgccgctttt gctgtatctg attttctata
    64201 ttatcttaat tcaatcgctt cagacttttc gcaatcgccg taatatttcc ccattttgtt
    64261 gtaaataaaa agcgtttacg caatgcaatc cttttgtaac aacccaggta aaacacctta
    64321 ttttcaagtt gttaaataag tggttattta cgcttttaac aaacgattga tggaatattt
    64381 tatgaaaggg aaatctgcat taactcttct gctcgctggt atattttcgt gcggcacatg
    64441 ccaggcg
//
LOCUS       DAZRUF010000002        57428 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZRUF010000002 DAZRUF010000000
VERSION     DAZRUF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.002"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            145..1488
                     /gene="argG"
                     /locus_tag="ACHXOL_000061"
     CDS             145..1488
                     /gene="argG"
                     /locus_tag="ACHXOL_000061"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR6807156.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1496..3121)
                     /locus_tag="ACHXOL_000062"
     CDS             complement(1496..3121)
                     /locus_tag="ACHXOL_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR6807157.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3580..3666)
                     /locus_tag="ACHXOL_000063"
     tRNA            complement(3580..3666)
                     /locus_tag="ACHXOL_000063"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3630..3632),aa:Leu,seq:gag)
     gene            complement(3681..4013)
                     /gene="secG"
                     /locus_tag="ACHXOL_000064"
     CDS             complement(3681..4013)
                     /gene="secG"
                     /locus_tag="ACHXOL_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR6807158.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4241..5578)
                     /gene="glmM"
                     /locus_tag="ACHXOL_000065"
     CDS             complement(4241..5578)
                     /gene="glmM"
                     /locus_tag="ACHXOL_000065"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR6807159.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5571..6419)
                     /gene="folP"
                     /locus_tag="ACHXOL_000066"
     CDS             complement(5571..6419)
                     /gene="folP"
                     /locus_tag="ACHXOL_000066"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR6807160.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6509..8443)
                     /gene="ftsH"
                     /locus_tag="ACHXOL_000067"
     CDS             complement(6509..8443)
                     /gene="ftsH"
                     /locus_tag="ACHXOL_000067"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR6807161.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8543..9172)
                     /gene="rlmE"
                     /locus_tag="ACHXOL_000068"
     CDS             complement(8543..9172)
                     /gene="rlmE"
                     /locus_tag="ACHXOL_000068"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR6807162.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9298..9591
                     /gene="yhbY"
                     /locus_tag="ACHXOL_000069"
     CDS             9298..9591
                     /gene="yhbY"
                     /locus_tag="ACHXOL_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR6807163.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9747..10223)
                     /gene="greA"
                     /locus_tag="ACHXOL_000070"
     CDS             complement(9747..10223)
                     /gene="greA"
                     /locus_tag="ACHXOL_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR6807164.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10471..11904
                     /gene="dacB"
                     /locus_tag="ACHXOL_000071"
     CDS             10471..11904
                     /gene="dacB"
                     /locus_tag="ACHXOL_000071"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR6807165.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            11959..12066
                     /locus_tag="ACHXOL_000072"
                     /pseudo
     CDS             11959..12066
                     /locus_tag="ACHXOL_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12090..13262)
                     /gene="cgtA"
                     /locus_tag="ACHXOL_000073"
     CDS             complement(12090..13262)
                     /gene="cgtA"
                     /locus_tag="ACHXOL_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR6807166.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13278..14243)
                     /locus_tag="ACHXOL_000074"
     CDS             complement(13278..14243)
                     /locus_tag="ACHXOL_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR6807167.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14370..14627)
                     /gene="rpmA"
                     /locus_tag="ACHXOL_000075"
     CDS             complement(14370..14627)
                     /gene="rpmA"
                     /locus_tag="ACHXOL_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR6807168.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14648..14959)
                     /gene="rplU"
                     /locus_tag="ACHXOL_000076"
     CDS             complement(14648..14959)
                     /gene="rplU"
                     /locus_tag="ACHXOL_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR6807169.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15218..16189
                     /gene="ispB"
                     /locus_tag="ACHXOL_000077"
     CDS             15218..16189
                     /gene="ispB"
                     /locus_tag="ACHXOL_000077"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR6807170.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16417..16695
                     /gene="sfsB"
                     /locus_tag="ACHXOL_000078"
     CDS             16417..16695
                     /gene="sfsB"
                     /locus_tag="ACHXOL_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR6807171.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16743..18002)
                     /gene="murA"
                     /locus_tag="ACHXOL_000079"
     CDS             complement(16743..18002)
                     /gene="murA"
                     /locus_tag="ACHXOL_000079"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR6807172.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18057..18311)
                     /gene="ibaG"
                     /locus_tag="ACHXOL_000080"
     CDS             complement(18057..18311)
                     /gene="ibaG"
                     /locus_tag="ACHXOL_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR6807173.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18471..18764)
                     /gene="mlaB"
                     /locus_tag="ACHXOL_000081"
     CDS             complement(18471..18764)
                     /gene="mlaB"
                     /locus_tag="ACHXOL_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR6807174.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18764..19399)
                     /gene="mlaC"
                     /locus_tag="ACHXOL_000082"
     CDS             complement(18764..19399)
                     /gene="mlaC"
                     /locus_tag="ACHXOL_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR6807175.1"
                     /translation="MFKRLMMVALLVIAPLSAATEADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19418..19969)
                     /gene="mlaD"
                     /locus_tag="ACHXOL_000083"
     CDS             complement(19418..19969)
                     /gene="mlaD"
                     /locus_tag="ACHXOL_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR6807176.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19974..20756)
                     /gene="mlaE"
                     /locus_tag="ACHXOL_000084"
     CDS             complement(19974..20756)
                     /gene="mlaE"
                     /locus_tag="ACHXOL_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR6807177.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20764..21573)
                     /gene="mlaF"
                     /locus_tag="ACHXOL_000085"
     CDS             complement(20764..21573)
                     /gene="mlaF"
                     /locus_tag="ACHXOL_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR6807178.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21783..22760
                     /locus_tag="ACHXOL_000086"
     CDS             21783..22760
                     /locus_tag="ACHXOL_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR6807179.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22774..23760
                     /gene="kdsD"
                     /locus_tag="ACHXOL_000087"
     CDS             22774..23760
                     /gene="kdsD"
                     /locus_tag="ACHXOL_000087"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR6807180.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23781..24347
                     /gene="kdsC"
                     /locus_tag="ACHXOL_000088"
     CDS             23781..24347
                     /gene="kdsC"
                     /locus_tag="ACHXOL_000088"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR6807181.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24344..24919
                     /gene="lptC"
                     /locus_tag="ACHXOL_000089"
     CDS             24344..24919
                     /gene="lptC"
                     /locus_tag="ACHXOL_000089"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR6807182.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24888..25445
                     /gene="lptA"
                     /locus_tag="ACHXOL_000090"
     CDS             24888..25445
                     /gene="lptA"
                     /locus_tag="ACHXOL_000090"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR6807183.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25452..26177
                     /gene="lptB"
                     /locus_tag="ACHXOL_000091"
     CDS             25452..26177
                     /gene="lptB"
                     /locus_tag="ACHXOL_000091"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR6807184.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26225..27658
                     /gene="rpoN"
                     /locus_tag="ACHXOL_000092"
     CDS             26225..27658
                     /gene="rpoN"
                     /locus_tag="ACHXOL_000092"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR6807185.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27681..27968
                     /gene="hpf"
                     /locus_tag="ACHXOL_000093"
     CDS             27681..27968
                     /gene="hpf"
                     /locus_tag="ACHXOL_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR6807186.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28086..28577
                     /gene="ptsN"
                     /locus_tag="ACHXOL_000094"
     CDS             28086..28577
                     /gene="ptsN"
                     /locus_tag="ACHXOL_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR6807187.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28623..29477
                     /gene="rapZ"
                     /locus_tag="ACHXOL_000095"
     CDS             28623..29477
                     /gene="rapZ"
                     /locus_tag="ACHXOL_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR6807188.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29474..29746
                     /gene="npr"
                     /locus_tag="ACHXOL_000096"
     CDS             29474..29746
                     /gene="npr"
                     /locus_tag="ACHXOL_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR6807189.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29960..30592
                     /gene="yrbL"
                     /locus_tag="ACHXOL_000097"
     CDS             29960..30592
                     /gene="yrbL"
                     /locus_tag="ACHXOL_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR6807190.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30589..31317)
                     /gene="mtgA"
                     /locus_tag="ACHXOL_000098"
     CDS             complement(30589..31317)
                     /gene="mtgA"
                     /locus_tag="ACHXOL_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR6807191.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31314..31967)
                     /gene="elbB"
                     /locus_tag="ACHXOL_000099"
     CDS             complement(31314..31967)
                     /gene="elbB"
                     /locus_tag="ACHXOL_000099"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR6807192.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32197..34533)
                     /gene="arcB"
                     /locus_tag="ACHXOL_000100"
     CDS             complement(32197..34533)
                     /gene="arcB"
                     /locus_tag="ACHXOL_000100"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR6807193.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34629..35558)
                     /locus_tag="ACHXOL_000101"
     CDS             complement(34629..35558)
                     /locus_tag="ACHXOL_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR6807194.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36233..40693
                     /gene="gltB"
                     /locus_tag="ACHXOL_000102"
     CDS             36233..40693
                     /gene="gltB"
                     /locus_tag="ACHXOL_000102"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR6807195.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40706..42124
                     /gene="gltD"
                     /locus_tag="ACHXOL_000103"
     CDS             40706..42124
                     /gene="gltD"
                     /locus_tag="ACHXOL_000103"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR6807196.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42308..42712
                     /locus_tag="ACHXOL_000104"
                     /pseudo
     CDS             42308..42712
                     /locus_tag="ACHXOL_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            42804..43109
                     /locus_tag="ACHXOL_000105"
     CDS             42804..43109
                     /locus_tag="ACHXOL_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR6807197.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43185..43649)
                     /gene="nanQ"
                     /locus_tag="ACHXOL_000106"
     CDS             complement(43185..43649)
                     /gene="nanQ"
                     /locus_tag="ACHXOL_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR6807198.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43646..44521)
                     /gene="nanK"
                     /locus_tag="ACHXOL_000107"
     CDS             complement(43646..44521)
                     /gene="nanK"
                     /locus_tag="ACHXOL_000107"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR6807199.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44518..45207)
                     /locus_tag="ACHXOL_000108"
     CDS             complement(44518..45207)
                     /locus_tag="ACHXOL_000108"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR6807200.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45255..46745)
                     /gene="nanT"
                     /locus_tag="ACHXOL_000109"
     CDS             complement(45255..46745)
                     /gene="nanT"
                     /locus_tag="ACHXOL_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR6807201.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46855..47748)
                     /gene="nanA"
                     /locus_tag="ACHXOL_000110"
     CDS             complement(46855..47748)
                     /gene="nanA"
                     /locus_tag="ACHXOL_000110"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR6807202.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47870..48661)
                     /gene="nanR"
                     /locus_tag="ACHXOL_000111"
     CDS             complement(47870..48661)
                     /gene="nanR"
                     /locus_tag="ACHXOL_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR6807203.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49041..50363
                     /gene="dcuC"
                     /locus_tag="ACHXOL_000112"
     CDS             49041..50363
                     /gene="dcuC"
                     /locus_tag="ACHXOL_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR6807204.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50406..50903)
                     /gene="sspB"
                     /locus_tag="ACHXOL_000113"
     CDS             complement(50406..50903)
                     /gene="sspB"
                     /locus_tag="ACHXOL_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR6807205.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50909..51547)
                     /gene="sspA"
                     /locus_tag="ACHXOL_000114"
     CDS             complement(50909..51547)
                     /gene="sspA"
                     /locus_tag="ACHXOL_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR6807206.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51942..52334)
                     /gene="rpsI"
                     /locus_tag="ACHXOL_000115"
     CDS             complement(51942..52334)
                     /gene="rpsI"
                     /locus_tag="ACHXOL_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR6807207.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52350..52778)
                     /gene="rplM"
                     /locus_tag="ACHXOL_000116"
     CDS             complement(52350..52778)
                     /gene="rplM"
                     /locus_tag="ACHXOL_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR6807208.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52997..54124)
                     /gene="zapE"
                     /locus_tag="ACHXOL_000117"
     CDS             complement(52997..54124)
                     /gene="zapE"
                     /locus_tag="ACHXOL_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR6807209.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54318..54716
                     /gene="zapG"
                     /locus_tag="ACHXOL_000118"
     CDS             54318..54716
                     /gene="zapG"
                     /locus_tag="ACHXOL_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR6807210.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54870..56237
                     /gene="degQ"
                     /locus_tag="ACHXOL_000119"
     CDS             54870..56237
                     /gene="degQ"
                     /locus_tag="ACHXOL_000119"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR6807211.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56327..57394
                     /gene="degS"
                     /locus_tag="ACHXOL_000120"
     CDS             56327..57394
                     /gene="degS"
                     /locus_tag="ACHXOL_000120"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR6807212.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14255 a        15306 c        14262 g        13605 t
ORIGIN      
        1 gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa
       61 tgaaaactca tttattttgc ataaaaattc agtgagagcg gaaatccagg ctcatcatca
      121 gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa
      181 cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga
      241 caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat
      301 tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac
      361 tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac
      421 accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt gactggtacc
      481 atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg tagcacctat
      541 aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc tgaactgcag
      601 atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg
      661 tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc
      721 acagactcca acatgcttgg tgcaacgcac gaagcgaagg atctggaata cctcaactcc
      781 agcgtcaaaa tcgtcaaccc aattatgggc gtgaaattct gggatgagag cgtgaagatc
      841 ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtagcgct gaacggtaaa
      901 acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc
      961 ctgggcatga gcgaccagat tgaaaaccgt atcatcgaag cgaaaagccg tggtatttac
     1021 gaagctccgg ggatggcact gctacatatc gcttatgaac gtctgttgac cggtattcac
     1081 aacgaagaca ccattgagca gtatcatgca catggtcgtc agttgggccg tctgctgtac
     1141 caggggcgtt ggtttgattc ccaggcgctg atgctgcgtg actctctgca acgctgggtt
     1201 gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga ttattcaatc
     1261 ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc
     1321 gactcggtgt tctcgccaga tgatcgtatt ggtcaattga ccatgcgtaa cctggatatc
     1381 actgataccc gagagaaact ttttggttat gccaaaactg gcctgctctc ctcctctgcc
     1441 acttcaggcg tgccgcaggt ggagaatctg gaaaacaaag gccagtaatt cgacatcaac
     1501 cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt ctgagcatca
     1561 cataatcgaa aacatcatgg tttgcatcca caacccgtga gtctccttta tagtgtgcaa
     1621 tcacttcctc cagcgttttc ggttgttccg gctttgttac ccccatccac gcattaataa
     1681 ggtaattatt gtaagctgtc gaaaagatgt tgttttccgt tgtacgatcc acgccatccc
     1741 caagaacggg gctataccag ataaacatcg ggacatgata tgcctgctgg ctagcctccc
     1801 tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa
     1861 aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat
     1921 ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacgg
     1981 cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa
     2041 tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc aacaacaatt
     2101 catcaaatcc tctgacataa actgtttcca tggcgcgcat ggcgatactg gtaactgctg
     2161 taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat
     2221 tagccatatt aataatgttg tcggggtaat tatgaatgtc atgactcaaa acagaatcag
     2281 cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc gccgcttatt gattgattaa
     2341 acagtttgat ctgttttctt tgtgcttcaa cttgcggtgt cgtagagcgt gtatatccat
     2401 acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac
     2461 ctgtatccct gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt
     2521 gatgctcttt cgctgctaaa gcaaaataat tgagattgaa aaacggcgta taggtagcaa
     2581 atcgcgacgc tagtatatat ggactgaatg cttttttatt ttttgcatct ttataagcaa
     2641 attgacaagc agaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt
     2701 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
     2761 aagcaaaagc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
     2821 cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
     2881 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
     2941 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
     3001 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
     3061 cacaaagata caacaaccaa tgagatttaa aacttctagc aaatttgttg aatactgtca
     3121 tccagaaatc caagttcaca accaatataa acaattatca acaatgttca attttatatc
     3181 agacatgttt ttcaactctg atccatatca gaccgaaaca ctgttagttt tttcttttta
     3241 aatgatgcgg ccattgcgtt ctcagtaatt aaataacatt atcaatgcgt ctctaaacat
     3301 tacgcagaac gtagccatca gtagttatac tgacaggagc aggatggttt atcgagaagt
     3361 aaacatatcc gtattcgtac cggaactgga gttgacacaa taaagtgcca attatgtcag
     3421 cagaagggaa aagtttataa ctaaggcgta ccggcaccat cgtttcaagg taccagctac
     3481 gagtaaagca actggacgag atacagatac ctgacaacca ttcctcagac caggaccaaa
     3541 acgaaaaaag acgcttttca gcgtctcttt tctggaattt ggtaccgagg acgggacttg
     3601 aacccgtaag ccctattggg cactaccacc tcaaggtagc gtgtctacca attccaccac
     3661 ctcggcacgg atactacttt ttagttcggg atatcgctgg tcggcttagc cggagcagct
     3721 ggctgagttt gttcggtttt cgccggtgca ctcagatttt cccattcgct acctttattg
     3781 gttttgttgc tattgatgtt acccagcacc agactgatga tgaagaataa cgttgccagc
     3841 agcgccgtca tgcgggtcat gaagttacca gaaccacttg aaccaaacag cgtagcggaa
     3901 gcgcctgctc cgaaggaggc tcccatatca gcgcctttac cttgctgcag catgatcaga
     3961 ccaacaaggc caattgccac aataaggaaa actaccaaaa gagcttcgta cataatcaac
     4021 ctgttccttg cggagttgcc gcgtaccaat gcttcaacca ataaagcggg agtttttaat
     4081 ctttcccact gaagtgggtg tgaatactaa ccaaagcgaa tgaccttcgc aagggcaatt
     4141 ttatcgcatt gtatcaactg cggaaaaaaa cagcaaaacc gatgtgttcg ctgagaaaaa
     4201 aggcgacaat cgccgccttt ttagccagtt atttaccgtt ttaaacggct tttactgcat
     4261 cggcgatacg gtgtgcaaat tcagttacct gcgcttcgtc ttcaccttct accatcacgc
     4321 gaattaacgg ttcggtgccg gatttacgca acagcacacg tccacggcta cccagcgccg
     4381 cttcaacttc ggcggtcaca gctttaactg actcatgctc aagcggatcg ccgctacctg
     4441 cggtgtaacg cacgttaacc agaatctgcg ggaacatttt catgccgctg caaaggtcgt
     4501 gcaggctcat atggttacgt gccatcgccg ccagcacctg caagccagca acgatgccgt
     4561 caccggtagt ggttttatcc agcaggatca catgaccgga attctctgca ccgatacgcc
     4621 agcctttctc ctgcattttt tccagtacgt agcggtcacc cactttcgcg cgcgcaaatg
     4681 gaattcctaa ctgtttcaac gccagctcaa gccccatgtt actcatcaat gtacccacag
     4741 caccaccacg cagctggccc tgacgaagac cttcacgcgc gatgatatac atgatctgat
     4801 caccatcgac tttattgcct tcatggtcaa ccataatcac gcgatcgccg tcgccgtcga
     4861 aggcaatacc gagatccgct ttttcagcca gcacacgagc ctggagcgcg cgaacgtcgg
     4921 tagcccccac ttcggcattg atgtttacac cgttcggctc acaaccgata gcgataacgt
     4981 tcgcccccag ttcacgcagc acgttcggcg cgatgtgata agtcgcaccg tttgcgcaat
     5041 ccaccacaat cttcagttcg ctgaggctaa gttcgttcgg gaacgtggct ttgcaaaact
     5101 cgatatagcg acccgcagca tcaacgatac ggctggcttt acccagttct gccgaatcaa
     5161 cacagctgat ctctttttcc atttccgctt cgatggcctc ttctaccgca tccggtagtt
     5221 tggtgccgtc gatagagaaa aatttaatgc cattatcgta gaacgggtta tgcgatgcag
     5281 atatcacaat tccggcctct gcgcggaagg tacgcgtcag ataagccacg gccggtgttg
     5341 gcatcgggcc agtgaagagt gcggaaaggc ccgctgccgc cagacccgct tccagtgctg
     5401 actccagcat atagccagaa atacgcgtgt ctttaccaat aatgatctta cgggagccgt
     5461 ggcgcgccag cactttaccc gcggcccaac ccagcttaag cacaaaatca ggtgtgatcg
     5521 gcgcatcccc tacacgacca cgaatcccat cggtaccgaa atatttacga ttactcatag
     5581 cgtttgtttt cctttgcaga cagagtggct tccaccaccc gcatcgcttc tacggtttct
     5641 ttgacgtcat gaacacgaat aatgtgcgcg ccttgcattg cggcaatgac cgcacaggcc
     5701 agactaccgc tcaggcgctc ggacggcccc acgttcagca gctgcccaat catcgatttt
     5761 cgtgacatac ctaccaacag cggcaggttg aaatggtgaa attcagccag gcgcgccagt
     5821 aatgaatagt tatgggagag atttttaccg aaaccgaatc cggggtcgag caacaatttc
     5881 tcttttgcga tacccgcctg ctcgcaacgt gctatttgct caataaagta gcgattcact
     5941 tctgcaaaga catcgtcata cttcggagct tcctgcatgg tttttggatt tccctgcata
     6001 tgcatcagac aaaccggtaa accggtttct gcagccgcct ccagagcgcc aggttcggaa
     6061 agggagcgga tatcgttaat aatgtgagcg ccaactttcg ctgactcacg gatgacttct
     6121 ggtttggatg tatctaccga gatccagact tcgaagcgtt gagcaattgc ctcaaccaca
     6181 ggaataacac gttgcaactc ttcttcaacg ctaacttccg ccgcccctgg gcgcgtggac
     6241 tcgccaccaa cgtcaatgat cgtcgcgcca gcgttgatca tcagattcgc atgtttcacc
     6301 gcatctatca gcgagttatg cgtgccacca tccgaaaagg aatcaggcgt gacgttgagg
     6361 atccccatta cgtgaggatg gctaaggtcc agtgaagtgc cctgggcaaa gagtttcatg
     6421 gtgttatccc tggtatgaat tgataagaaa aaaccccgga gcacgccccg gggttttcgg
     6481 tacaaataca gtcatctgat gcgggaactt acttgtcgcc taactgctct gacatggtgt
     6541 tacccgggtt cggcgtacgc ggttcatcaa ccggacgagg agcctttgga ctaccattgt
     6601 cgccagaatt gttagaagcg cctggttctt cccagcccgc tggcggacgt acatcgcgac
     6661 gtgccatcag gtcatcaatc tgcggtgcgt cgatagtctc atatttcatg agagcatctt
     6721 tcatcgcatg cagaatatcc atattgtcgg tcagaagctg acgcgcacga ttatagttac
     6781 gctcaatcag tgctttcact tcctggtcga tgatacgtgc agtttcatcg gacatatgtt
     6841 tcgctttcgc tacgctacgg ccgaggaaca cttcaccttc ttcttccgcg tacagcagtg
     6901 gacccaattt ctcagagaag ccccactgag tcaccatgtt acgcgccagg ttggtcgcaa
     6961 ctttaatatc gttggacgca ccggtagaaa catgttccgg cccgtagatg atctcttctg
     7021 ccagacgacc accgtacagc gtagaaatct ggctttccag tttctgacgg ctggcgctga
     7081 ttgcgtcgcc ctcaggcaag aagaaagtca cacccagcgc acgaccgcgt gggataatcg
     7141 tcactttgtg caccggatcg tgttccggca ccaggcgacc gataatcgca tgacccgctt
     7201 cgtggtaagc cgtcgattct ttctgcgctt ccgtcatcac catggagcga cgttccgcac
     7261 ccatcatgat tttatctttc gctttctcga actcaaccat cgacacaacg cgtttgttgc
     7321 cacgagcagc gaacagtgcc gcttcgttta ccaggttcgc caggtcagca ccggagaaac
     7381 caggagtacc acgggcaatg attgccgcgt cgatatcggg tgccaatggt acgcgacgca
     7441 tgtgaacttt caggatctgc tcacgaccgc gaacatctgg caagccaacc acaacctgac
     7501 ggtcgaaacg gccaggacgc agcagggccg ggtcaagaac gtccggacgg ttagtcgcgg
     7561 cgataacgat gataccttcg ttaccttcga agccatccat ctcaaccagc atctggttca
     7621 gagtctgttc acgttcatcg tgaccaccgc ccagaccagc gccacgctgg cggcctacgg
     7681 cgtcgatttc atcgataaag atgatgcacg gtgccgcttt cttcgcctgt tcgaacatgt
     7741 cacgaacacg ggatgcaccc acaccgacga acatttctac gaagtcagaa ccggagatag
     7801 taaagaacgg aactttcgct tcgcctgcaa tcgctttcgc cagcagcgtt ttaccggtac
     7861 ccggaggacc gaccatcagg acgcctttcg ggatcttacc gccgagtttc tggaagcggc
     7921 tcggctcgcg cagatattca accagttcag caacttcttc ttttgcttcg tcgcagcccg
     7981 caacgtcagc aaaggtcgtt ttgatctgat cttccgtcag catgcgcgct ttgctcttac
     8041 caaacgacat ggcacctttg ccaccgccgc cctgcatttg acgcatgaag aagatccaca
     8101 caccaatcag caacagcatc gggaaccagg agatgaagat agaagccagc aggcttggtt
     8161 cttcaggcgg ttcaccgaca accttgacgt tcttggtcaa caggttatcc agtaatttcg
     8221 gatcctgaac cggaatgtaa gtggtataac ggttactatc tttcttggta acgttgattt
     8281 cacgtccgtt gatacgcgct tcacgaacct ggtcgttatt gacctcttgt aggaaggtag
     8341 agtaatccac cttacggcca ttagactcgc tgggcccaaa gctctggaat actgacatca
     8401 gcacaacggc aatgaccagc cagagtatta ggtttttcgc catgtcactc aagggattaa
     8461 cctcttatta caactgtgtt aaaaacagcg tcaggatact ctatatccag catctttcaa
     8521 actttcgtct gaaatctccc ggttagggtt tacgcccggt cgctacaata tacacttccc
     8581 gcgaacgtgc acgagaagag tccggcttac gaactttgac cttcgtaaac agggagcgaa
     8641 tttcccttag atactcatcg aaaccttcgc cctggaacac cttcactaca aaactgccac
     8701 ctggcgctaa tacatcacga cacatttcta gcgccagttc caccagatac atggcacggg
     8761 ggatatccac cgccggtgtt ccgctcatgt ttggtgccat atcggacatg acaacctgga
     8821 ctttgctgtc gccaacgcgc tccagcagtg ctttcatcac cagttcatca cgaaaatcgc
     8881 cctgaagaaa gtccacacca acgataggat ccataggtaa aagatcgcaa gcgatgatgc
     8941 ggcctttgcc gccaatttgg gtgaccacat attgtgacca accacccgga gcagcaccaa
     9001 ggtcgacaac cgtcattccc ggtttaaaga gtttgtcact ttgctgtatt tcatcaagtt
     9061 taaaccaggc acgggaacgt aacccctttt tctgtgcctg ttgaacatat ttatcgctaa
     9121 agtgttcctg aagccagcgg ctggagctgg cagaacgctt cttacctgtc atttaacttt
     9181 cccatgggga taactcatcg taaccaattg cgtaaatttt tacgcgccta tttggtgata
     9241 tatgggagat ggcggtagaa tgacccattt tcaatcccaa cgtaagcaaa aaatacgatg
     9301 aatctgagta ctaaacaaaa acagcacctg aaaggtctgg cacatccgct caagccagtt
     9361 gttctgcttg gcagtaatgg tttgaccgaa ggggtgctgg ccgagattga acaagcgtta
     9421 gagcaccatg aactcatcaa ggtgaaaatc gccaccgaag atcgcgaaac aaaaaccttg
     9481 atcgtggaag ctatcgtgcg cgaaaccggc gcctgtaatg tacaggtcat cggtaaaacg
     9541 ctggtgcttt atcgcccaac taaagaacgt aaaatctcgc tgccacgcta agattatcct
     9601 aaagttacac acatttgctg tgtaaaacga ggggttttcc gcaggcagga gagcaaaatg
     9661 ccacgctctg ttcgttgata aaaggccgca tagcggcctt tttcctttct ttacaataca
     9721 tcaacatctt gagtattggg taattcttac aggtattcca ccttaattac ttcaaattct
     9781 acttcgccgc ccggcgtttt gatgaccaca acatcatctt cttctttgcc gatcaggcca
     9841 cgagcaatag gcgagtttac agaaatcagg ttttgtttaa agtcagcttc gtcatcgcca
     9901 acgatgcgat aagtctgttc ttcgtcagaa tccagattca gcaccgttac ggtagcacca
     9961 aaaataacgc gcccattgtt aggcattttg gtgacatcaa tcacctgcgc gtttgacagc
    10021 ttggcttcga tgtctttgat acggccttcg cagaagccct gctgttcacg agctgcgtgg
    10081 tattcggcgt tttctttcag gtcgccatgc tcacgcgctt ccgcgatagc agcaatgatt
    10141 tcaggacggc gcacagattt cagaaaatcc agctcttcgc gtaatttttc agcgccgcgt
    10201 aaggtcatcg gaatagcttg catttgttat acctcttgaa tattcctgat ggggcaagtc
    10261 ttcacccacc ccggctgttc ggcctgcccg gcataactcc tcaccgggac cagaagcaaa
    10321 aaaataccga cccaggtaca agtcccaggt cagctacaat tcacattttg atagtcattt
    10381 taccctgaag ttcccgaagg gtcatcgttt actttatagg gcgttgcgcc gtagtatgac
    10441 ggctcgattc caggttgtta gcgcgagatt atgcgatttt ccagatttat catcggattg
    10501 accagctgta tagcgttcag tgttcaggcc gcaaatgttg atgagtacat tactcaactc
    10561 cccgctggtg ccaaccttgc cctgatggtg caaaaagtcg gcgcgtcggc ccccgctatt
    10621 gattaccaca gtcagcagat ggcgctgcct gccagtaccc agaaagtgat tactgcgctg
    10681 gcggcgttga ttcaactcgg ccccgatttt cgttttacca cgacgcttga aaccaaaggc
    10741 aatgtggaaa acggcgtact taagggtgac ttagtggcgc gatttggtgc cgatccgacg
    10801 ttaaaacgtc aggatattcg caatatggtc gcgactttga aaaaatctgg cgtcaaccaa
    10861 atcgatggca atgtgttgat agatacctcc attttcgcca gccacgataa agcccccggc
    10921 tggccatgga atgacatgac acaatgcttt agcgctccgc ctgccgccgc catagttgac
    10981 cgcaactgtt tctccgtctc gctctacagt gcccaaaaac ccggtgatat ggcctttata
    11041 cgcgtggcat cttattaccc cgttacgatg ttcagccagg tacgcaccct cccccgtggt
    11101 tctgccgaag cgcaatactg cgaactggat gtcgtaccag gcgacctgaa ccgctttacg
    11161 ctgacgggat gcctgccaca acgttctgag ccgctcccgt tggcttttgc cgtgcaggat
    11221 ggagccagtt atgccggtgc aattctgaaa gatgagttaa aacaggcggg tatcacctgg
    11281 agcggaacac tgctgcgcca gactcaggtt aacgaacctg gaacggtagt tgccagtaaa
    11341 cagtcggccc cgctgcacga tctgcttaag attatgctga aaaagtcgga taacatgatc
    11401 gccgatacgg ttttccgcat gataggccat gcgcgcttca atgtacctgg tacatggcgg
    11461 gccgggtcgg acgctgtgcg tcagatcctg cgccagcaag ccggtgtcga tattggaaac
    11521 accattattg ccgatggttc agggctttcg cggcataacc tgattgcccc cgccaccatg
    11581 atgcaggtgc tgcaatacat tgcccaacac gacaatgaac ttaactttat ctccatgctg
    11641 ccgctggcgg gctatgacgg ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg
    11701 gatggaaaag tctcagcgaa aaccggttcg ttgcaggggg tatataacct ggcgggattc
    11761 attaccacag cgagcgggca acgaatggcg tttgtgcaat atctttctgg ctatgcagta
    11821 gaacctgcgg atcagcgtaa tcgccgtatt ccgttagtgc gttttgaaag ccgtttgtat
    11881 aaagatattt atcagaacaa ttagtcaaaa agaaaccccg gcacatggcc ggggcttcag
    11941 attattgaca aagtgcgctt tgtttatgcc ggatgcggcg taaacgcctt atccggccta
    12001 caaaatcgtg caaattcaac atattgcaat tctcttgtag gcctgataag cgtagcgcat
    12061 caggctgatt tggcgtttat catcagtgat taacgcttgt aaatgaactc aacgccttct
    12121 tcgtcgtctt cgtcccagtc gtcatcccag tcttcatcat cctcttcagc aatctcttca
    12181 agctgctggc gatgataatc gtcccacatg aattcgactt tctctggctg tttcgcttct
    12241 tcagcctgca cgaccgggtt ttcaaggata aaggtcatca catcccagca gagatctttc
    12301 acgcccagtc cgctcgccgc agagatcaga taatatttat cttcccagcc cagcgcttca
    12361 gcaatcgctt tcgctttctc ttcagcttct gccttatcca gcagatcgat cttattgaac
    12421 actaaccaac gcggtttcgc cgccagatcc tggctgtatt tttccagctc gctgataata
    12481 atacgcgcgt tttcaaccgg atcggtgccg tcaatcggat cgatatcgat gaggtgcaac
    12541 aggacgcggc aacgttccag gtgcttcagg aagcggatac ccagacctgc gccttccgca
    12601 gcgccttcaa tcagtcctgg aatatcggca acaacgaagc tcttttcgtt gtccattcgt
    12661 accacaccca gacttggcac cagagtggta aacggataat ccgccacttt cggtttagcc
    12721 gccgataccg cgcgaataaa ggtcgattta cccgcgtttg gcatccccaa cataccgacg
    12781 tcagccagca gcatcagctc cagcagcagc tcacgcttat cacccggcgt accgttggtt
    12841 ttctgccgtg gtgtacggtt aacggacgat ttgaaacggg tattgcccag accgtgccag
    12901 ccgcccttag caaccagcag acgctgaccg tgtttagtca tatcgcccat ggtttcacca
    12961 gtaccctggt cgattacacg cgtacctacc ggcactttaa tcgtcacgtc tttaccgcgc
    13021 ttaccggtac aatcgcggct tgcgccgttc tgaccgcgct ctgcacggaa agatttttca
    13081 aaacgataat cgataagcgt gttcaggttt tcgtcggctt ccatccatac gtcaccacca
    13141 tcaccaccgt cgccgccatc cgggccgcct ttcggaatat acttttcgcg gcggaagctc
    13201 acgcaaccat taccgccatc acctgcaacg accagaatcg atgcttcatc aacaaacttc
    13261 attttattct ccataaatca ttcgcctgcg cggggttgcg ataccaccgt tgtatgctta
    13321 cgtaatccgc cccaaatacg atgaccaatg gcggaataca tcgcgcccgc aaccacgaca
    13381 aacgcaccga gataacctaa aaggtttaac atcggtctgg cgaagaaatc gggccaggcc
    13441 agtgataaaa gatctgaaaa aaacagcgta aacagtgggg tgagcgtgat gatcgcgctc
    13501 acctgcgctg cctgccagcg agccatcgct tccgccaggg cgccatatcc taccaatgta
    13561 ttcagtccgc aaaaaattaa acatgcgagc tgccagtggc taagctgcgc tatcactcca
    13621 ggctttgcca gagggaagag cgcaattgta cataaagcgt acagtaaaaa caggatctgc
    13681 ggtgaggcca gccgacgcaa taaaaccttt tgcgccacgc cataactcac ccaaaccgtc
    13741 gccgcaccga ccccaaagat aactccccag gtgtaatcgg tgagctttgt aaatatctcg
    13801 accagactgg tgttaaaaaa catcaccagg ccgctcagga gcatcaatgc ccctacaacc
    13861 tgagtgctgc gcattttctc tttcaggata aatacgctgg caaccatcat gccaactggc
    13921 gagagttgcc caatcacctg cgaagcggtc ggactcaggt attgcaagga tgagctgaac
    13981 aggatgaagt tcccaaacag cccggcggtc gccactgcca acaaaatcaa ccagcgtggc
    14041 ttacgaaaca cgcgtaatgg cggcaacctc ttcttcaccg caagaatggc accaaggcca
    14101 atactcgcca tcaagaaacg gtaaaacacg attgtcggag gttccatcac ctccagcacc
    14161 tgcttcattg cgattggcaa cgccccccag caaattgctg tggtgagcgc caaaagaatg
    14221 ccaatgcctg cctgctgctt cataccgtat tccctgcaaa gcgcatttgc ggattaacgt
    14281 cgcaccatgc gcatttttac cggttatcga atgtaaaaag ccccgcaacg tgttgcgggg
    14341 ctttcatccg ttaccgggac gcgaaaaact tattcagctt cgatgctgat aaatttacgg
    14401 tttttcgggc ctttaacttc gaatttcact ttaccgtctg ctttagcaaa cagagtgtgg
    14461 tcacgaccgc aacctacgtt agcgccagcg tggaatttgg taccacgttg acgaacgatg
    14521 atgctacccg ccagaacgga ttcgccaccg aaacgcttaa cgcccaggcg tttagcttct
    14581 gaatcgcgac cgttacgtgt ggagccgcca gcctttttat gtgccatttg aaatctctcc
    14641 tcaggtctta ggcgctgatg ccagtaattt tcacatcagt gaaccactga cgatggccct
    14701 gctgcttacg atagtgttta cgacgacgaa acttaacgat tttaactttc tcgccacgac
    14761 cgtgagcaac aacttcagct ttgattacgc cgccatcaac gaaaggaacg ccgattttga
    14821 cttcttcacc gtttgcgatc atcagcactt cagcgaactc aacagtttcg ccagttgcga
    14881 tgtccagctt ttccaggcga acggtctgac cttcgcttac tcggtgttgt ttaccaccac
    14941 tttggaaaac cgcgtacata taaaactccg cttccgcgca caccttttca atgattcaga
    15001 gtgcgctata aatattcaca atagggcgcg aatattacgc aaaacgcacg cctttgacaa
    15061 gtgctacagt caatacacga agaaaaaaaa cacaacttgt acggtaacgt ttatctgtgc
    15121 cattttttca gtacaatcac cctatattcc taaccataaa ccctaagttg cctttgttca
    15181 cagtaaggta atcggggcga aaagcccggc ttttgcgatg aatttagaaa aaatcaatga
    15241 gttaaccgcg caagatatgg cgggtgttaa tgcggcaatc cttgagcagc ttaattccga
    15301 cgtccaactg atcaatcagt taggctatta catcgtcagc ggcggcggta aacgtattcg
    15361 tccgatgatt gctgtactgg ctgcacgagc tgttggctat gagggaaatg cgcatgtcac
    15421 cattgctgcc ctgatcgagt ttatccacac ggcgactctg ctacacgacg acgttgtgga
    15481 tgaatcagat atgcgcaggg gtaaagctac cgccaacgcc gcatttggca atgccgccag
    15541 cgtgctggta ggcgatttta tttatacccg cgcgttccag atgatgacca gcctcggttc
    15601 actcaaagtg ctggaagtca tgtcagaagc tgtaaacgtc atcgcagaag gtgaagttct
    15661 gcaactgatg aacgttaacg atccggacat cactgaagaa aactacatgc gcgttatcta
    15721 tagcaaaacc gcgcgtctgt ttgaggctgc cgcgcagtgt tccgggattc tggctggctg
    15781 tacgccggag gaggagaaag gcctgcagga ttatgggcgc tatctcggca ctgctttcca
    15841 gttgatcgac gatttactcg attacaatgc cgatggcgaa cagttaggta aaaatgtcgg
    15901 cgacgatctg aacgaaggta aaccgacgct gccgctgctg catgcgatgc atcatggcac
    15961 accagaacag gcacagatga tccgtaccgc catcgaacag ggtaacggtc gccatcttct
    16021 ggaaccggtt ctggaagcaa tgaacgcttg tggatctctt gaatggacgc gtcagcgtgc
    16081 cgaggaagaa gcagacaaag ccatcgcagc attacaggtg ctcccggaca ccccttggcg
    16141 agaagcactc atcggcctcg cgcacatcgc tgttcaacgc gatcgttaat cccctccctt
    16201 catcccgcgc agtgcgcggg atgattccag taaattccat aaaaacacta attcagcgct
    16261 ataacacctg aaaaaaaggg cgatgaatct ttatatattc tgaatattca cactctttac
    16321 aggaactttt tagagcaata ggccatcagg agtatagtga tgcccgacag aagaagtgtt
    16381 ctgaatgaaa gcgactaact taaggagtga ggaaagatgg aaagtaattt cattgactgg
    16441 catcccgctg acatcattgc gggtttgcgc aagaagggaa cgtcaatggc ggcggagtct
    16501 cgcagaaatg gtttgagttc ctcaacgctg gcaaacgcat tatcgcgccc atggccgaaa
    16561 ggagagatga ttattgcgaa agccctggga actgacccct gggttatctg gccatcacgc
    16621 taccatgatc cgcagaccca tgagtttatc gacagaacgc agttgatgcg tagctacact
    16681 aaaccgaaaa aatgagtggt ctggcggtag ccccgcgaac ggggctgcca gctctcagac
    16741 gattactcgc ctttcacacg ctcaatattt gcacctaaag cgcgcagttt gtcttcaatg
    16801 cgttcgtagc cacgatcgat gtgataaata cgatcaacca ccgtcgtccc ttccgcaata
    16861 cagccagcca gcaccaggct tgctgatgca cgcagatcgg ttgccataac ctgtgcgcca
    16921 gaaagttttt caacaccgtg acaaataacg gtattgcttt cgatttcggc atgcgcgccc
    16981 atacggctaa gctccggcac atgcataaag cggttttcaa agaccgtttc ggtgatgaat
    17041 ccggtccctt ctgccaccag gttcaacagc gtgaactggg cctgcatatc ggtcgggaat
    17101 gccggatgcg gcgcggtacg tacgttaaca gccttcggac gtttgccatg catatccagg
    17161 ctaatccagt cttcgccgac ttcgatgtcc gctccagcgt cacgcagttt cgccagcacg
    17221 gcgtcgagag tatctggctg cgcgttacgg cagataattt tgccgcgaga aatcgccgcc
    17281 gccaccagga aagtaccggt ttcgatacga tccggtagca cgcgatagac accgccgcct
    17341 aaacgttcca caccttcgat gacgatacga tcggtgccct gaccgctaat tttcgcaccc
    17401 agcgtaatca ggaagttcgc ggtatcgacg atttccggtt cacgcgctgc gttttcaata
    17461 atcgtggtac cttccgccag ggttgcagca cacatgatgg tcaccgttgc gccaacgctg
    17521 actttatcca tcacgatatg tgcgcctttc agacgaccat cgacggaagc tttaacgtaa
    17581 ccttcttcca gtttgatggt cgcgcctaat tgttcgaggc cagaaatgtg tagatcaacc
    17641 ggacgcgcac cgatcgtaca accgccaggc agtgaaactt gcccctgacc aaagcgcgct
    17701 accagcggcc ccagtgccca gatagaagca cgcatggttt taaccagatc gtaaggtgcg
    17761 cagaatacat taacgtcgcg ggcatcaata tgcacagaac cattacgttc tactttcgca
    17821 cccagctggc ttagcagctt cattgatgta tcgacgtctt tcagttttgg gacgttctgg
    17881 atctctaccg gttcttccgc cagtagagcg gcaaaaagga taggcagagc agcattttta
    17941 gcgccggaaa ttgtgacttc gccctggagc ttcgttggcc cctgaacacg aaatttatcc
    18001 atttagtttg ttctcagtta acaattcata tccgctaccg gcgaatcgcc catagctcaa
    18061 aagccgttca gtttgcgatc gcgcgcccac tccgcagggg tatacgcttt gatcgacaca
    18121 gcatgaatgc ggttatccgc aatatattcc atcagcgggc catagaccgt ctgctgtttt
    18181 ttaacccgac tcatgccgtc aaacaactca cccacggcaa taacctgaaa gtggctgcca
    18241 tcgccggaaa cgtggacttc ctggagggag agagcgttca tcaacacgct ctgaatttca
    18301 ttattttcca tgggatcttc aatcatcagt taataaacca gcgaaacatc ttagagcaaa
    18361 gttgcgctgg cataaataag caaaaagcct cgctgataaa tcagacaagg ctcgacttgc
    18421 aggcaggttt gccggacagg cggttaacgc catatccggc ctgaaaaaat ttaacgaggc
    18481 agaacatcag caggcaaatt atacaatttc gcgagggtat acactttgtc gtttaccccc
    18541 tgaagcgtca cgttgttgcc ctgctttttc gccagatcga taagatggag cagcagtgcc
    18601 agtccacccg tatccacgcg ggacacacgg ctaagatcga tgcaggtaat ccccttcacc
    18661 gcttcctcac gcatttccca aagcggtagc agaacgtcct gatccagctc tccggataac
    18721 gccagcgtgt cacccgtctg catccagctc agtgactcgc tcattatttt ttctcttcca
    18781 gagtgatttt ctgttgagaa atcgatttca gttgcgcagt caggccgtcg atacctttgg
    18841 tacgcagcag cgttccccac tcgttttgtt tggtggtgat catactgacg ccttcagcaa
    18901 tcatgtcgta agcctgccag tttcccgtct gggagttttt acgccactgg aagtccagac
    18961 gcaccggcgg acggccattc gggtcaataa tggtaacgcg aataggcaca atggttttat
    19021 cgcccagcgg ctgttctggc gcaatctgat aggtttgacc gtgatacatc gccagcgcct
    19081 gaccgtaagc ctgcttcagg tactcacgga aagcggcaaa gtaggcttca cgttgagcag
    19141 gggtcgcact cttgtaatac tggcccagca ccagcgcacc ggcgtatttc acctgtacgt
    19201 atggcagcag ttcctgatca acaatggtac gcagataatc cgggttggcc cgaatttgcg
    19261 gttgctcatt cttcaggcga tcgaacgttt tctgcgccgc ctcgtccatc agcttataag
    19321 gattggtctg gtctgcctcg gttgccgcac tcagaggtgc aatcaccagc aaagcgacca
    19381 tcattaaacg tttaaacatg cgtcggttct cctgaaatta tttcgttgta cccacaggtt
    19441 cagtggtttc attattacct ggcgcagcag ctggcgcatc gccactattc ttattgtcat
    19501 cgcctttact accgtaaagg aactgaccaa tgagatcttc cagcaccatc gcagacttag
    19561 tgtcctgaat tgtatcgcca tccttcagga tagcagtccc cagttccggg tcttcaaaac
    19621 cgacgtttaa tgccagatat tgttccccca gcaggccgga agtacgaatg ctcagcgaac
    19681 tggtatctgg aatgtggtta taacgttgtt caatttccag cgttacgcgc ggcagatagg
    19741 ttttcgggtc cagcgtaata tccgccaccc gacccacaac aacgccacca atactgaccg
    19801 gagagcgcgc tttcaggccg ccaatgttat cgaacgtcgc ataaagcgtg taggtcggtt
    19861 cagtacgtat ggacgtcacg ttcgccgcct tcaggcaaac aaacagcgcc gccagcagtg
    19921 ctgctaataa aaagataccc acccaaattt catttttttt cgtttgcatg aactcaattc
    19981 ccaaacatca atgcggtcag cacaaaatcc agccccagaa cagccagaga cgagtggaca
    20041 acggtgcgag tggttgcccg gctaatcccg gcagacgtcg ggatggcgtc gtagccgtta
    20101 aacaacgaaa tccacgtcac cgtgatggcg aacaccacgc tcttaatcag acagttgacc
    20161 agatccatac gccagtcgac ggcattttgc attgccgacc agaagaaccc gctatcaatg
    20221 cctttccaac tgacgccgac cagagatccg ccccagatcc ccacggcgac gaaaataacc
    20281 gtcaacagtg gtaatgaaat aaccccagcc cagaaacggg gagaaataac ccgacgcagc
    20341 ggatccaccg ccatcatctc catactggag agttgctctg tagcgcgcat caggccgatt
    20401 tctgcggtta gcgccgaacc agcacgcccg gcaaacaaca acgcggcaac caccggcccc
    20461 agttcacgca gtagcgataa cgccaccagc atacccagac tggtttccgc actataagtg
    20521 gtcagaacca gataaccttg cagccccaac accattccga tgaacacgcc agaaaccaca
    20581 ataatcagca tcgacaggac gccgacatta tagagctggc gcaccagcag cggcgcatgt
    20641 ttgcgaaatt ccggtttgcc gaccagcgca ttgaataaca ttaacccggc ccgcccgaac
    20701 gttctcaggg ttttaatccc tttatgtccg agcgacgcca gcgcatttaa cagcatgagt
    20761 ggcttaactc cctggtaaaa gatcagcgtg ataatcgccg gcaggatagc ggaacggaac
    20821 aggcccgtca gctatcccgt ccagaaactg acgtacgcgc ggatcaggat tcgcctgcaa
    20881 cgcctgggca ctgccatgag cgacaatttt tttgtccgcc aggatccagg cgtgatccgc
    20941 aatacttaac acttccggca catcgtgaga aaccaccaca caagtcacgc ccagcgcgct
    21001 gttcagctca gaaatcagct tcaccagtac gcccatggtg atgggatctt gcccaacaaa
    21061 aggttcatca aacatgatga gatccggctc cagcgcaatc gcacgcgcca gcgctgcacg
    21121 ccgcgccatc ccaccggaaa gttcagaagg cattagttta gccgctccac gcagccccac
    21181 ggcctccagc ttcatcatca ccgtactatg caacaatggc gcgggtagtt gggtatgttc
    21241 gcgcagtgga taggcgacgt tgtcaaatac gttcatatca gtgaacaacg ccccggactg
    21301 aaataacatg ctcatccgtt tgcgcactgt atacaggcgc gaacgagaca tcgccggaat
    21361 attctcacca tcgaaaagga tctcaccatg atctggtgcg atttgcccgc caatcagacg
    21421 gagtagcgtc gttttaccga tgcccgatgg ccccatgatc gccgtgatct tccctcgcgg
    21481 cacggtcagg gaaatattat cgaagatgca gcgattgcca cgcgtaaaac tgacatcgcg
    21541 catatcgact aaattcgcca cagactgctc cataattcac ccttcgtctt gcgttgattt
    21601 tctaagcatg gcgctcaatt taaccttgaa cccaacatat ttacagaata ttacccgccg
    21661 tggttagcga aagctggcat ttgttttact ttttagccgc ataaagtcaa aattaagaat
    21721 ccgttacggc tttctgaaaa tcttcagcgg accgacgagt atacctgaag aaaggacgtt
    21781 agatgctttt agctacggca ctgttaattg ttggtttact tttggtcgtt tacagtgccg
    21841 accgcctggt ttttgccgcg tctattcttt gccgaacctt tggcatcccg ccgctgatca
    21901 tcggcatgac ggtggtcagt attggtacat cgttaccaga aatcatcgtc tcgcttgccg
    21961 cgtctctgca cgaacaacgc gatttagccg ttggtacagc cctcggctca aacattatca
    22021 atattttgct gatcctcggt ctggctgcgc tggttcgtcc ttttaccgtc cattccgatg
    22081 ttctgcgtcg tgaattaccc ttaatgttgt tggtcagcgt agtggccggt tccgtactct
    22141 atgacggaca acttagtcgc agcgatggta tctttctcct ctttctggct gtgctatggc
    22201 tgctgttcat tgttaaactt gcacgtcagg ctgaacgtca ggggactgac agcctgacca
    22261 gagagcagct tgcagagctg ccgcgtgacg gcggattgcc cgtcgcgttt ttatggctcg
    22321 gcattgcgct tatcatcatg ccagtggcca cgcggatggt ggttgataac gccacggtgc
    22381 tggcgaatta ctttgccatc agcgagttga cgatgggtct gacggcaatt gctatcggaa
    22441 ccagcctgcc ggaactggca accgcaatag cgggggttcg caaaggtgaa aacgacattg
    22501 ctgtcggaaa tatcattggc gcaaacattt ttaatattgt catcgtgttg ggtttacccg
    22561 cgctgataac gccaggagag attgagccat tggcgtacag tcgtgactac agcgtgatgt
    22621 tgctggtgag cattattttt gcgttgctgt gctggcggcg ctccccgcaa ccgggccgtg
    22681 gtgtaggggt attattaact ggcggattta tcgtatggct ggcgatgttg tactggttat
    22741 cgccaatact cgttgaataa ctggaaacgc attatgtcgc acgtagagtt acaaccgggt
    22801 tttgactttc agcaagcagg taaagaagtc ctggcgattg aacgtgaatg cctggcggag
    22861 cttgatcaat acatcaatca gaatttcacg cttgcctgtg aaaagatgtt ctggtgtaaa
    22921 gggaaagttg tcgtcatggg gatggggaaa tcggggcaca tcgggcgcaa aatggccgca
    22981 acgtttgcca gcaccggtac accttcattt ttcgtccatc ctggtgaagc cgcgcatggt
    23041 gatttaggca tggtcacccc acaggatgtg gtgattgcta tctctaactc aggtgaatcc
    23101 agcgaaatca cggccttaat tccagtgctt aagcgtcttc acgtaccgtt aatctgcatc
    23161 accggtcgcc cggagagcag catggcgcgc gccgcagatg tgcatctgtg tgttaaagta
    23221 gcgaaagaag cctgtccgtt agggctggca ccgaccagca gcaccaccgc cacgctggtt
    23281 atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc tgaagatttt
    23341 gcgctctcac acccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt aaacgatatt
    23401 atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg tgacgcgttg
    23461 ctggaagtta cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa tatgatgatt
    23521 gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg atatgggcgt ggatgttcgt
    23581 cagttaagta ttgccgatgt gatgacgccg gggggaatac gtgtgcgccc tggcattctg
    23641 gccgttgagg cactgaactt aatgcagtcc cgccatatca cctccgtgat ggttgccgat
    23701 ggcgaccatt tactcggagt gttacatatg catgatttac tgcgtgcagg cgtagtgtaa
    23761 agattcaagg ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc tgttacggcc
    23821 ctgtcagcgc cgacgttata gcaaaagcag agaacattcg tctgctgatc ctcgatgtcg
    23881 atggcgtact gtcagatggc ctgatttata tgggcaataa tggcgaagag ctgaaagcgt
    23941 tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac ctctgatatt gaagtcgcta
    24001 tcattaccgg gcgaaaggct aaactggtag aagatcgttg tgccacattg gggatcactc
    24061 acttgtatca ggggcagtca aacaaactga tcgcctttag cgatctgctg gaaaaactgg
    24121 cgattgcccc ggaaaatgtg gcttatgtcg gcgatgatct catcgactgg ccggtaatgg
    24181 aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc actgttgatc ccgcgcgccg
    24241 attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg cgaagtttgc gacttattac
    24301 tcctggcgca gggcaaactg gatgaagcca aagggcaatc gatatgagta aagccagacg
    24361 ttgggttatc attgtgctat cactggcggt tctggtgatg atcggcattg atatggccga
    24421 aaaagacgat accgcccagg tggtcgtcaa caacaatgat cccacctata aaagcgagca
    24481 tacggacacg ctcgtctata acccagaagg ggcactaagc tatcgattga ttgctcaaca
    24541 cgttgaatat tattccgatc aggccgtttc gtggtttacg cagccggtac ttaccacgtt
    24601 tgataaggat aaaatcccga catggtccgt aaaagcagat aaagccaagc tgaccaatga
    24661 ccggatgctc tatttatatg gacacgttga agtcaacgca cttgtgccag actctcaact
    24721 tcgcagaatc acgacggata acgcgcagat caatctggtg acgcaggatg ttacctctga
    24781 agacctcgtc acgttatacg gaacaacatt taactccagc ggtctgaaaa tgcgcggcaa
    24841 cttacgcagc aagaacgccg agctgattga aaaggttaga acatcctatg aaattcaaaa
    24901 caaacaaact cagccttaat cttgtgcttg ccagctcact tctggccgcc agcattccgg
    24961 catttgccgt aaccggagac actgatcagc cgatccacat tgaatcggac cagcaatctc
    25021 ttgatatgca aggcaacgtg gttaccttta ccggtaatgt catcgtcacc cagggcacca
    25081 tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg cggcgaacaa ggtaaagaag
    25141 tgattgacgg ctacggtaaa ccggcaacgt tctaccagat gcaggacaac ggtaaacccg
    25201 ttgaaggtca cgcttcccag atgcactacg aactggcaaa agattttgtc gttctgacgg
    25261 gtaatgctta tctgcagcag gtcgatagca acattaaggg cgataagatc acttacctgg
    25321 taaaagagca gaaaatgcag gctttcagcg acaaaggcaa gcgcgtaacc accgttctgg
    25381 tgccgtcgca gctgcaggac aaaaacaaca aaggccagac cccggcacag aagaagggta
    25441 attaattcgt tatggcaaca ttaactgcaa agaaccttgc aaaagcctat aaaggccgtc
    25501 gcgtggtaga agacgtcagc ctgaccgtca actccgggga aattgtcggt ctgctggggc
    25561 caaacggtgc cggtaagacc accactttct acatggttgt aggcattgtg ccgcgcgatg
    25621 cgggcaacat cattattgat gatgacgata tcagtctgct gcctctgcat gcacgcgcgc
    25681 gccgcggtat cggctatctg ccacaggaag cctccatttt ccgtcgcctc agcgtttacg
    25741 ataacctgat ggcggtactg caaattcgtg acgacttgtc tgctgaacaa cgtgaagacc
    25801 gcgcgaacga gctgatggaa gagtttcaca ttgagcacct gcgtgacagc atggggcagt
    25861 cactctccgg gggtgaacgt cgccgtgtag aaattgcccg cgcactggct gcgaatccga
    25921 aatttattct gctcgacgaa ccgtttgccg gggttgaccc gatctcggtt atcgacatta
    25981 aacgcatcat tgagcacctg cgcgacagcg gcctgggcgt gctgatcact gaccacaacg
    26041 tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt cagtcagggg catttgatcg
    26101 cccacggcac gcctacagaa atcttacaag acgaacacgt taagcgtgta taccttgggg
    26161 aagacttcag actctgatag ggtagaagtt tgcgacgttt tagcaggaga gtacgattct
    26221 gaacatgaag caaggtttgc aactcaggct tagccaacaa ctggcgatga cgccacagct
    26281 ccaacaggca attcgtctgt tgcagttgtc gacgctggaa cttcagcagg agctacagca
    26341 ggcgctggag agtaatccgc tgcttgagca aatcgacact catgaagaaa tcgacacccg
    26401 cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg ctcgaacaaa aagagatgcc
    26461 ggaagagctg ccgctcgatg ccagttggga caccatttac accgctggta caccatccgg
    26521 caccagcggt gactacattg acgatgagct gccggtctat cagggcgaaa cgacgcagac
    26581 cttgcaggat tacctgatgt ggcaggtcga gctgacaccg ttttccgaca ctgaccgcgc
    26641 tattgctacc tctatcgtcg atgccgttga tgacatcggt tatctgactg tcccgctgga
    26701 agatattctc gaaagtatgg gcgatgaaga gatcgacatc gacgaggttg aagccgtcct
    26761 taagcggatc caacggtttg atccggtcgg tgtagcggca aaagatctgc gtgactgcct
    26821 gctaatccaa ctctcccaat tcgataaaac cacgccgtgg ctggaagagg ccagactgat
    26881 cattagcgat catctcgatc tgttagccaa tcacgacttc cgcactttaa tgcgcgtcac
    26941 gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg atccagtcgc tcgatccgcg
    27001 ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc attccagatg tgctggtgcg
    27061 taagcataac ggtcactgga cggtagaact caacagtgac agcattccgc gtctgcaaat
    27121 caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac gatggtgaca gccagtttat
    27181 ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt ctggaaagcc gtaacgatac
    27241 gctactgcgc gtgagtcgct gtatcgttga acagcagcaa gccttctttg agcaaggtga
    27301 agaatatatg aaaccgatgg tactggccga tatcgcccag gctgtcgaaa tgcatgaatc
    27361 gacgatatct cgcgtgacca cgcaaaaata cctgcatagt ccacgaggca tttttgaact
    27421 gaagtatttc ttttccagtc acatcaatac cgagggcggc ggcgaagctt cctccacggc
    27481 gattcgtgcg ctggtgaaga aattaatcgc ggcggaaaac ccagcgaaac cgttgagcga
    27541 cagcaagtta acctctttgc tgtcggaaca aggtatcatg gtggcacgcc gcactgttgc
    27601 gaagtaccga gagtctttat ccattccgcc gtcaaaccag cgtaaacagc tcgtttgacc
    27661 caaccgataa ggaagacact atgcagctca acattaccgg aaataacgtc gagatcaccg
    27721 aggcactgcg cgaatttgtt acagccaaat ttgccaaact tgagcaatat tttgaccgaa
    27781 tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac ccacacctca gatgcaacac
    27841 tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg tcaggacatg tacgctgcca
    27901 ttgatggctt aattgataag ctggcacgcc agttgaccaa acataaagat aaactgaaac
    27961 aacactaatt gtccgggcaa ttagcatgtg catggcggcc tgttgtgcgg cacaacgggc
    28021 catttgtacg gttaatgctc cgagcctgtt ccactgtttg agtgggcagg ttcttaggtg
    28081 aaattatgac aaataatgat acaactctac agcttagcag tgttcttaac agggaatgta
    28141 cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct ggaaatcatc agcgagctgg
    28201 cggcgaaaca acttagcctg ccgcctcagg tggtttttga agctatcctg acgcgggaaa
    28261 aaatgggcag taccggtatc ggcaatggta ttgccattcc gcatggcaaa ctggaagaag
    28321 atactctgcg cgccgttggc gttttcgttc agctcgaaac gcctatagct ttcgatgcca
    28381 tcgacaacca accggtggac ttactttttg ccctgctggt gtcggcagac caaactaaaa
    28441 cgcacctgca tactctgtcg ctggtggcga aacgtctggc ggacaaaacc atctgccgcc
    28501 gtttacgcgc agcccagagc gatgaagagc tgtatcaaat cattacggat accgaaggta
    28561 ctccggatga agcgtagtta ttcggtaatg tctcttttag acgttgtgag gagaaacagt
    28621 acatggtact gatgatcgtc agcggacgtt caggttcagg taaatctgtc gccctgcgtg
    28681 cgctggaaga tatgggtttt tactgcgtgg ataaccttcc cgtagtgttg ttacccgatc
    28741 tggctcgaac cctggccgat cgcgagattt ctgccgccgt cagcattgat gttcgtaata
    28801 tgccggagtc accagaaata ttcgaacagg cgatgagtaa cctgcctgac gctttctcac
    28861 cgcaactcct gttcctggat gccgaccgta ataccttaat tcgtcgttac agtgacacgc
    28921 gccgactgca tccgctttcc agcaaaaacc tgtcgctgga aagtgctatc gacaaagaaa
    28981 gcgatttgct ggagcctctg cgttcgcgag cggatctgat tgtcgatacc tcagaaatgt
    29041 ccgttcacga gctggctgaa atgctgcgta cccgtctgct gggtaaacgt gaacgcgaac
    29101 tgaccatggt ctttgagtct ttcggcttca aacacggtat ccctatcgat gcagattacg
    29161 tctttgacgt gcgcttcttg ccgaacccgc actgggatcc gaaactgcgt ccaatgacag
    29221 gtcttgataa acctgtcgcc gcgttcctcg accgccacac agaagtacac aattttatct
    29281 accagacgcg aagctatctt gagctatggt tacctatgct ggaaaccaac aaccgtagct
    29341 acttgacggt cgccattggt tgtaccggcg ggaagcaccg ttcggtgtat attgcagagc
    29401 aactggcaga ctacttccgc tcgcgcggta aaaacgtcca gtcacgccat cgtacgctgg
    29461 aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc acaaacaagc tgggcatgca
    29521 tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt tttgacgctg aagtgctgtt
    29581 acgtaatgac gaaggcaccg aggccgaagc caacagcgtt attgcgctgc tgatgttgga
    29641 ttctgccaaa ggacggcaga ttgaagttga agcgaccggc ccacaggaag aggaagcact
    29701 ggccgccgtt atcgccctct ttaactctgg ttttgatgaa gattaatctt catcactttg
    29761 acatacaact cccttcaaac tccccccgac aataagaaaa tcacgtactg aaatcgttct
    29821 caatcaacgt catttgtaca ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac
    29881 cttaagaggc attgtttagg ttttgtttaa gttaatcgac catactggag atcgtcagaa
    29941 aatatttcca ggagatggca tgattcgctt atctgaacaa agtcccctgg gcaccggaag
    30001 acatcggaaa tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga ttgtctacca
    30061 tcgtggcgat ggtggcgata aagagatccg ccgtgagtta aagtactacg cgcatcttgg
    30121 tcgccggtta aaagactgga gtggaatacc gcgctatcac ggtaccgttg agacggattg
    30181 cggaaccggt tatgtctacg atgtgattgc agattttgac ggcaaactct ccattacgct
    30241 gaccgaattt gctgaacaat gccgttacga agaagacatt gcacaactgc gccagctact
    30301 gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca
    30361 gaatatcctc tgtcaccgca tcagtgagtc agaggttatc ccggtcgtct gcgacaacat
    30421 tggtgaaagc acattgattc cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca
    30481 agagcggtta tggaaacgat ttatcgccca accggcgctg gctattgccc tgcagaaaga
    30541 tctgcaacct cgtgagagca aaacgctggc cctcacttcg cgcgaagctt aatccagctg
    30601 gtgttgttgc ataaacggct caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg
    30661 acggctacgc acgtagcccg atggcgagga gactttgaaa cgaagtggat taggtaatac
    30721 agctgccagt aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt ggaaataacg
    30781 ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat tcggcgatat tcaggtaaac
    30841 cgtcaggata cgctttttgc tccagactgt ttctatcccc agcgttaatc cagcttccag
    30901 cccttttcgt acccagctac gcccatccca taaaaagaga tttttggccg tctgctgaga
    30961 aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca gggctttctc
    31021 aatggaagcg acatcaaagc cccagtgctc aggaaatttc tgatcttctg cggcaatcac
    31081 cgccagtccc atccacggcg agatttgatc catactgacc cagtcagaat gtgccacgta
    31141 acgaaaattg ccatgcagcc aggcgctgac ctgtcgctcg accattaccg ctgagaaggg
    31201 aacaggcgca acactaaaca acgcgatgcc cccgccccag aaaacggcga ggacaaccat
    31261 taacagcaaa agaaaacggc gaacgaaact aaacaccgtt aagcggcttt tactcattca
    31321 gccagaacca gcacgcggga aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc
    31381 tgcgccagca tatatgctgg ggtggtgaca atcttattgt cttcatcaac cacgatatca
    31441 tcgacaggac agggcacatg ctccgcgccc atctcttcca gcacttctgc ggtatcgata
    31501 tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat cgccggggca
    31561 atacacataa aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag cgcctttaat
    31621 tcacggtcaa cggtgcattc gctaccaaga gtggcaaaat tgcttaaatt cttcgccgcg
    31681 ccaaaccccc ccggcacaat caacgcatcc agttcagcgg catcggcctg ggccagagga
    31741 cggatttcac cgcgcgttat tcgtgccgct tcaatcagca cgttgcgcgt ttccgtcatc
    31801 gcttcgccag ttaaatggtt gataacatca acctgctgct tatccggtgc aaagcagacc
    31861 gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg aatttcagaa
    31921 ccgtcataga cgccgcatcc gctcagaatt acgccaattt tcttcattgt gatcatcctt
    31981 tcgcaattga ctgaaacaca ttaatttttt taataaaaat ggtacgcatc acacatttaa
    32041 ctgattcatg taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac
    32101 agtgctgtgc ccttgtaact catcataata atttacggcg cagccaagat ttccctggtg
    32161 ttggcgcagt attcgcgcac cccggtctag ccggggtcat tttttagtgg cttttgccac
    32221 ccacgctttc agcacttcta cgtcgtgacg ccactcttct ttcatctctt caatccattc
    32281 accgacgtta tcttcccagg caggaaggtc aggagactga atttgctgac ccagctgttg
    32341 cagatggcgt aaccccactg accccgccgc acctttaatt ttatgtcctt cctcaacaat
    32401 gcctttttta tcctgcgccg tcagattcga ctccagcacg ctgacatagc ccggcatcat
    32461 cttctcaaac accgctaacc cgtcggtgat cagcttcggt cctacaagtt cgagatactg
    32521 ttccagcatg ggaatatcga gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt
    32581 actctcctca tcatcctggg tatcccagaa tttcttgatc atcgcggtta gcgccggaac
    32641 agaaagcggc ttactcagca catcatccat tccagcattg aggtactctt gtttgtcttt
    32701 cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc tctcgcggat aacgcttcgt
    32761 cagttcacga gagatatcca gcccggtcat atctggcaac tgaatatcca gtaacaccag
    32821 gtcgtattcg cccggtttaa acatctctag cgccgccttg ccggtcatgg cgacatcaac
    32881 gctgttacct aatttttcca gcacagaacg cgcaacaatc acgttcagtt caatgtcttc
    32941 caccagcagc acattcagcg ccggtaaagg catatcgtct tcatcaaacg catcatcgac
    33001 ctcttctgct accgacggtg cgtggatcgt caacgtaaag gttgaacctt tgccctgttc
    33061 gctggtaacc gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa cggccagacc
    33121 aataccggtg ccggtggcag gtttaccgcc atgactgtct ttcacctggt aatacatggc
    33181 gaaaatttta tccagctcat cctgcggaat gccgatacca gagtcttcca cttcaaaatg
    33241 cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc tggccttgct gggtgaattt
    33301 gacggcgtta ctgatgaggt tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac
    33361 ctgatgcggt aatggcagcg tcggctccag gttaaagcgc aatccttttt gttgcgcctg
    33421 caatgcggag agattttcca gatcggcaag gaagctggtg aaatcaaccg gttgattatc
    33481 aagctggacc ttgcgccgtt ccatcttatc catgtcgata atatcgttaa agatattccc
    33541 cagcgtgacg gccgaaacat ggatggtctt gagatatttt tcctgctcgg cggtgagttc
    33601 ggtatccagc agaatgcggc tcagaccgac gataccgttc agcggtgtac gcaattcgtg
    33661 actgatggtg gagataaacg tcgttttgtc gcggctggcc cgttcaagcg catcctgata
    33721 ccgcttacgc tcggtaatgt ctcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg
    33781 gtcgtagtac ggcactttac ggatttcaaa gcaggctttg cgcccgtccg ggtaatccag
    33841 ccactgttca taggtcagtg acacattatg acggaacact ttttcatcgg tttcaatgac
    33901 ttttgcggcg gcttccggtg agtaaacatc agcaggtttc aggtgaacca gttgtttttc
    33961 gctttttccg gtcagcagct ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt
    34021 acgataaaaa accaggtcgg gtgaagcatc aaggaaggaa cgtaagaatg aggattgctg
    34081 ctcgagctga atttgtgtct cttcgcgctc tttgatttca attttcagtt ggccgaaggt
    34141 ttcctgcagt tctgcttccg ctttttcacg aacggcgatt tcctgattta gctgggcaat
    34201 attatctttt aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg
    34261 caccagccgt gacagacgtt gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa
    34321 gtagaccgcc cacggcgtaa tcaacaaacc aaagaagata gaacgaataa catcaatgct
    34381 ttcgacctga ccatgcagca ccatggttac cgccatttgt accacaatgg caagaacgac
    34441 gagggccagc gccagcaaca ttgagaagcg caccagacct aacttcatca tcaggtcaac
    34501 ataatactgc gccagcagac gaatttgctt cattagggaa ttccttcacg acaacctgcc
    34561 ccaataatac ccaattctga cagttacgtt ataaattgtg tgagaaatgc ggaattcccc
    34621 tcaacgacct actccgttgg aggtagccac ggacgtccca gcgccgatcc ctgtacgcca
    34681 tgttcattca gatagcgatc cagctcgacc attcccgtcc atcgattttc gcaccacaga
    34741 ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat cacttccggt
    34801 ggcgtatggc gaatcatttc tccggcagtg agcgtgtaat cctccagttc aataccgttc
    34861 aaacgccccg cttcccaggc tttcgccata atgctgcctt tcacaatatg caacggatgt
    34921 agctttatac cgtcaacacc ggtttccact acccgctcca gcgtttgcag acattcagcc
    34981 agcccttcgc ctggcagacc gacaattaaa tgcgagcaca ccttcagccc gcgctggcgt
    35041 gccagctgcg tcgtgcgctg ataacaggcg aaatcatgac cgcgattaat gcgatgtagc
    35101 gttttgtcgt gcacggtttg cagccccagc tccagccaca cttcgtagcc ctggtcttta
    35161 tattcgcaaa gcagatccag caccgcatcc ggcacgcagt ccgggcgcgt gccgacacac
    35221 aaaccgacaa tattggcctg gctcaccgcc tgttgataca tcgaacgcag cacctgaact
    35281 tcggcaaagg tgctggtata cgcctggaaa taggcgaggt aacgtttcgc gcgattaatt
    35341 aaattcgcct gatgcgccag ctgttcggca atggaacgat gctgctgcgc ttcgtcggca
    35401 aacgaggcaa cattacagaa ggtgcagccg ccgcgcccga tggtgccgtc gcgattcggg
    35461 caactgaaac cgccgtgcag cgtcagctta tgcacctttt gcccataacg acgggtgaga
    35521 tcaccaccaa acatattgac taatttctgt aactgcataa tctgatagac cgcgccttga
    35581 aaagaggcca aagcctgcca tttttagctc aattcggcga tgacctggat caatcgtccc
    35641 ggcccgcttt tatcaactgc ataatcaatc aaaattaccg aaatttcatg cataatcaca
    35701 taaatcactt ttgcttatct tgtgtcagat ttttttatct ccttatggat tttaggcaaa
    35761 aacagtagca tgaaacgtca ttaccaatta atgcagtata aaatgctggt tttctcgtca
    35821 attcaaggca ggataagggt taacacgcct ttatgacagt caggaattga ctgtttctct
    35881 aacgacttcc cttttagcct taaagataaa atctatttta atttcagcca tttaataaag
    35941 aattttgcgc taaagcacat ttctgtacca ataagcttgc catttgacct gtatcagctt
    36001 tcccgataag ttggaaatcc gctggaagct ttctggatga gcagcctgct catcatattt
    36061 atgcagtaat tgagatcccc tcttcaccgt attaaccgat gcgaaaagga caacaagggg
    36121 gcgaatgcga ggcgcgcgta tgacacgcaa cccccgtcgc cacgctcttt ctgtacccgt
    36181 gcgcagcggt tcggtagtgg ggttcccgca gagcctgggg gaggttcacg atatgttgta
    36241 cgataaatcc cttgagaggg ataactgtgg tttcggcctg atcgcccaca tagaaggcga
    36301 acctagccac aaggtagtgc gtactgcaat acacgcactg gcccgcatgc agcaccgtgg
    36361 cgcgattctc gccgatggta aaaccggtga cggttgcggc ttgctgttac aaaaaccgga
    36421 tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt ttagcgaaaa actacgccgt
    36481 cgggatgcta ttcctgaata aagatcctga actcgccgct gccgcacgcc gcatcgttga
    36541 agaagaactg caacgcgaaa ccttgtcgat tgtgggctgg cgtgatgtcc ccactaacga
    36601 aggcgtactg ggtgaaatcg ccctctcctc tctgccccgc attgagcaaa tttttgtgaa
    36661 cgccccggca ggctggcgtc cgcgcgatat ggagcgccgt ctgtttatcg cccgccgccg
    36721 cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc tgtagcctat cgaatctggt
    36781 gaacatctat aaaggtctgt gtatgccggc ggatctgccg cgcttttatc tggatcttgc
    36841 ggacctgcgt ctggaatcgg ccatttgcct gttccaccag cgcttctcca ctaacaccgt
    36901 accgcgctgg ccgctggcgc aaccgttccg ctatctggcg cataacggtg aaatcaacac
    36961 catcactggt aaccgccaat gggcgcgtgc gcgtacctat aaattccaga cgccgcttat
    37021 ccctgatctg cacgacgccg caccgttcgt caacgaaacc ggctctgact ccagttcgat
    37081 ggataacatg ctggaactcc tgctggcagg cgggatggat atcatccgcg ccatgcgtct
    37141 gttagtgcca cccgcctggc agaacaaccc ggatatggac ccggaactgc gtgccttctt
    37201 tgactttaac tccatgcata tggagccgtg ggatggcccg gcgggcatcg tgatgtccga
    37261 cggtcgtttt gccgcctgta acctcgaccg taacggtctg cgtccggcgc gctacgtcat
    37321 caccaaagat aagctcatca cctgcgcctc tgaagtcggt atctgggatt accagcctga
    37381 cgaagtggtc gaaaaaggcc gcgtcgggcc aggcgaactg atggttatcg acacccgcag
    37441 tgggcatatt ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc atccatataa
    37501 agagtggatg gagaaaaacg tccgccgact ggtaccgttt gaagatctgc ccgatgaaga
    37561 agtgggtagc cgcgaactgg acgacgacac gcttgccagc taccagaaac agtttaacta
    37621 cagcgcggaa gagctggact ccgtaattcg cgtactgggc gaaaacggtc aggaagcggt
    37681 cggttcgatg ggcgatgata ccccattcgc cgtgctctcc agtcagccgc gcattattta
    37741 cgactacttc cgccagcagt ttgcccaggt gactaacccg ccaatcgacc cgctgcgtga
    37801 agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct tttgcgaagc
    37861 agagggccag gcgcaccgtt taagctttaa atcgccgatt ctgctctact ccgatttcaa
    37921 acagctcacg acgatgaaag aggagcacta ccgcgcagat acgctggata tcacctttga
    37981 cgtcactaaa accacgctcg aagcgacagt caaagagctg tgcgacaaag ccgaaaaaat
    38041 ggtacgtagc ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcta aagatcgcct
    38101 gccggttcca gccccgatgg cggttggcgc gatccagacc cgtctggtcg atcaaagcct
    38161 gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc cgcgatccgc accacttcgc
    38221 cgtgttgctg ggcttcggcg cgacggctat ttatccatac cttgcctatg aaacgctggg
    38281 ccgcctggta gacacccatg cgattgccaa agattatcgt accgtgatgc tcaactaccg
    38341 taacggcatc aacaaaggct tgtacaaaat catgtccaaa atgggcatct ccaccatcgc
    38401 ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtcta cacgatgatg tagtgggcct
    38461 gtgcttccag ggggcggtca gccgcattgg tggggcaagc tttgaagact tccagcagga
    38521 tctgctgaat ctgtcgaaac gtgcctggct ggcgcgtaag cccatcagcc agggcggcct
    38581 gctgaaatac gtccacggcg gcgaatacca cgcctacaac ccggacgtgg tgcgcacgct
    38641 gcaacaagcg gtacaaagcg gcgagtacag cgactatcag gaatacgcga agctggttaa
    38701 tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg aaaacgcggt
    38761 caacattgct gatgttgaac cggcaagcga actgtttaaa cgcttcgata ccgctgcgat
    38821 gtctatcggc gcgttaagcc cggaagccca cgaggcgctg gcggaagcga tgaacagcat
    38881 cggcggtaac tcaaactccg gtgaaggcgg tgaagatccg gcgcgctacg gcaccaacaa
    38941 agtgtcgcgc atcaagcagg tggcttccgg tcgctttggg gttactccgg cgtatctggt
    39001 caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg aagccgggcg aaggcggtca
    39061 gttgccgggt gataaagtca ctccttacat cgccaaactg cgctattcgg tgcccggagt
    39121 gacgctgatc tccccgccgc cgcaccacga tatctactct atcgaggact tagcgcagct
    39181 cattttcgac ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc tggtttccga
    39241 accgggcgta ggcaccatcg cgactggcgt ggcaaaagct tatgccgatt taatcaccat
    39301 cgcaggctat gacggtggca ccggcgcaag cccgctgtcg tcagtgaaat acgcaggttg
    39361 cccgtgggag ctggggcttg ttgaaaccca gcaggcgctg gttgccaatg gtctgcgtca
    39421 taagatccgt ttgcaggtcg atggcggcct gaaaaccggt gttgatatca tcaaagcggc
    39481 gattctcggc gcagaaagct tcggcttcgg cactggccca atggtcgcgc tcggctgtaa
    39541 atatctgcgt atttgccacc tgaacaactg cgcaacgggt gtggcaactc aggatgacaa
    39601 actgcgtaag aatcactatc acggcctgcc gttcaaggtg acgaattact ttgagtttat
    39661 cgcccgtgaa acccgcgagc tgatggcaca gctgggggta acgcggctgg tggatctgat
    39721 tggtcgcacc gacctgctga aagagctgga cggtttcacc gccaaacagc agaaactggc
    39781 gctgtcgaag ctgctggaga ccgccgaacc gcatccaggt aaggcactct actgcaccga
    39841 aaacaacccg ccgtttgata acggcctgct gaacgcgcag ttgctgcaac aggcgaaacc
    39901 gtttgtcgat gaacgccaga gcaaaacctt ctggttcgat atccgcaata ccgatcgctc
    39961 cgtgggcgcg tcgctttcag gctatatcgc ccagacgcac ggcgatcagg ggctggcagc
    40021 cgatcctatc aaagcgtact tcaacggcac cgcaggccag agtttcggtg tatggaacgc
    40081 gggcggcgtg gaactgtacc tgaccgggga tgccaacgac tatgtcggta aaggcatggc
    40141 gggcggctta atcgccattc gtcctccggt tggttccgcc ttccgcagcc atgaagcaag
    40201 tattatcggc aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg
    40261 cgcgggtgaa cgtttcggcg tgcggaactc cggtgctatc accgtggtag aaggcattgg
    40321 cgacaacggc tgtgaatata tgacgggcgg tattgtctgt attctgggta aaaccggcgt
    40381 taacttcggt gcgggcatga ccggcggctt cgcttacgtt ctcgatgaaa gcggtgattt
    40441 ccgcaaacgc gttaacccgg aactggtcga ggtcttaagc gttgacgatc tggcgatcca
    40501 tgaagagcat ctgcgcggtc ttatcaccga gcatgttcag catactggct ctcagcgcgg
    40561 tgaagagatt ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg ttaaaccgaa
    40621 gtccagtgat gtaaaagcac tgctgggtca tcgtagtcgt agcgcagcag agttgcgcgt
    40681 gcaggcgcag taaggggtag caacaatgag tcagaatgtt tatcaattta tcgacctgca
    40741 gcgcgttgat ccgccaaaga aaccgctgaa gatccgcaaa attgagtttg ttgaaattta
    40801 cgagccgttt tccgaaggcc aggccaaagc gcaggctgac cgctgcctgt cgtgcggcaa
    40861 cccatactgc gagtggaaat gtccggtaca caactacatc ccgaactggc tgaagctcgc
    40921 caacgagggg cgtatttttg aagcggcgga actgtcgcac cagaccaaca ccctgccgga
    40981 agtttgcgga cgagtctgcc cgcaagaccg tctgtgcgaa ggttcctgca ctctgaacga
    41041 tgagtttggc gcggtgacca tcggcaacat tgagcgttat atcaatgata aagcgttcga
    41101 aatgggctgg cgtccggata tgtccggcgt gaagcaaacc ggtaaaaaag tggcgattat
    41161 cggcgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg acgcgtaacg gcgtaaaagc
    41221 cgttgtcttc gaccgtcatc cagaaattgg cgggctgctg accttcggta ttccggcctt
    41281 caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc ttcaccggca tgggtattga
    41341 attcaaactc aataccgaag tgggccgcga cgtgcagctg gacgatctgc tgagtgatta
    41401 cgatgccgtc ttccttggcg tcgggactta tcagtcaatg cgcggcgggc tggaaaacga
    41461 agacgccgat ggcgtgtacg cagcgctgcc gttccttatc gccaacacca aacagttaat
    41521 gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtggt
    41581 ccttggcggt ggcgacactg cgatggactg cgtgcgtacg tccgtgcgcc agggagcgaa
    41641 gcacgttacc tgtgcctatc gtcgtgatga agagaacatg ccgggttccc gccgcgaagt
    41701 gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac gtccagccac tggggattga
    41761 agtgaacggt aacggcaaag tcagcggcgt aaaaatggta cgtaccgaaa tgggcgaacc
    41821 ggatgccaaa ggccgtcgcc gcgcggagat cgttgcaggt tccgaacata tcgttccggc
    41881 agatgcagtg atcatggcgt ttggtttccg tccacacaac atggaatggc tggcaaaaca
    41941 cagcgtcgag ctggattcac aaggccgcat catcgccccg gaaggcagcg acaacgcctt
    42001 ccagaccagc aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt ccgatctggt
    42061 ggtaaccgct attgccgaag gtcgtaaggc ggcagacggt attatgaact ggctggaagt
    42121 ttaagctctg tcccagcgcc gcctcctcag aaaagagtcg gcgctgaaat tctcctttct
    42181 ttctggaaag cgcctcgcat aatctgaaga aaaccaatgt aatacgctca tctggattgt
    42241 taccggtaga tgcccggcgt atagctgatt ccattattca acgcagcgcc atataaaggg
    42301 acagataatg gaatcgctct cagagagaac ctcaacaggc taccagcaaa tccacgacgg
    42361 tattattcac ctggtcgata gcgcccggac ggaaacggta cgtagcgtta acgcgctgat
    42421 gaccgcgaca tactgggaaa ttggccgacg aattgtcgaa tttgaacaag gtggcgaggc
    42481 aagggctgcg tatggtgcgc agctaatcaa gcgactatca aaggatttaa gtctaaggta
    42541 taagcgtggt ttctctgcaa aaaacttacg ccaaatgagg cttttttacc tcttttttca
    42601 acatgttgaa attcgccaga cagtgtctgg cgaattaaca ccattgggaa ttccgcagac
    42661 accatctgca gaattcccat cggcaaagat ttggcagacg ctgtctgcca aattgagagg
    42721 ctgtttgata tttccacagc ttttgtattg catctcctat tttcggtttg tatattcatg
    42781 caaataaacg aaggaaagtt gccgtgattt acattgaaca gcttgaatta atccataaaa
    42841 gtggtgatgt gttgtatcca gtaaaaataa ccagaaaatc atcaggaaaa actgccttcc
    42901 accttgttcc attcggctta aataagactc acgaccttct agaggtggaa gatgcttcag
    42961 aagcaattcg gctggtgatt gatgaacgtc attccattcg ctgcagtaca cttacagcaa
    43021 ctattacgaa taaaaagggc aagcgaatta agcggacagg tatatacagt atcaagggtg
    43081 ttaacataaa agaatacaac gtcaggtaat ggcacagagc ggtccgtcag ttcaggtttg
    43141 gctctgtgcc aaagatgggt caggtcacac ctgatctaat aaatttaagc cattaaatca
    43201 gccttaacct tcaccaccac ctttttaatc tcacccggct cgccaacaac gcatcccggc
    43261 ttatgcggct ctcctagcat aaacacggcg aacattcccg gtttcaggat gatggcttgc
    43321 tcgttttcaa tggtgctgca aagctggtaa tcatcctcat ggtggaactc ttcacactga
    43381 cgcgcagtgc ctgccatgcc aaacagaatc cgttcttcac cgtttaataa cagctggata
    43441 tcaatgtatt gctcgtgcag ctccgctttt ttctcaacgg gcgattgagt gttaaacgtc
    43501 atgacattca taaaaatatt gtcgccctgt aattcgtaac gacccggcgc tttttcttgc
    43561 ggtctggcag ctaatgccag cgttaacgcg tcctgtaacg caggatgtaa cccagcagac
    43621 ggtaatgacc gtacttcacc catcatcata atttttctcc ctgggccaac agcgctgccc
    43681 caagtaaacc tgcatcatgg cggtagtgcg ccgccagtaa atcaacatga aataccgctg
    43741 gctcctgcgc cagatacgtt tccaccaacg ccagataccc ttctgccaga ccaacgctgc
    43801 caccgaccac cacgcactgg caatcagttg tggctttaat atcagcgatc agccttgcca
    43861 gcacatgtgc ggagcggtga atcagttgct gcgcctgctc gtcgccctgc ccggcgcgtg
    43921 tgaaaatagt tctcgcatcc gcgcccatga gttcaccttg cgctgccgct gcaataccgc
    43981 gaccagaagc aatcgcttcc acgcaacctg tgcgtccaca gccgcagact gggccgtgtg
    44041 gatcggcaag cgtatgcccg atatgccccg ccagaccgcc agggccggta agcagtttac
    44101 cgccgctcac tacaccgccg ccgacgccag tggaaacggt gataaacacc atctcggtta
    44161 catcgccttc cagcgcctga tactctgccc atgctgcggc ctgcgcgtcg ttaatggcaa
    44221 tggtcggcaa attggtaagt tgttccagcg ttttgactaa cggaaagtgt agcaatccac
    44281 cgagattatg cggattcagc gccagcaaac tgccgtcgcg aataattccg gttgaagcga
    44341 tggcaacccg ctgcgcatga gcttgcaacg gagagactaa tgcggataag gcatcacgca
    44401 aggcttctgg tgtctggctg gctggcgtag gaagttcacg acgatcgcgg atctgcccgt
    44461 cagcgccaat cagcgcggcg gcaagtttag taccgccgat atcaatcgcc agtgtggtca
    44521 tagcaccgcc tttttcatcg ctgtgttgta ccactgacaa atgtgttcaa gacgcgtaat
    44581 ggcagaaccg accgtcaccg cccacgcgcc gtggcgcatc gcatctgccg cctgagcagg
    44641 cgtgttgtaa cgcccttcgg caatcacccg acatccggct tcgctcaacg ttttcaccag
    44701 cgccagatcc ggctcttctg gcgtttcagg cgtggtatag ccagaaagcg tagtgccaat
    44761 aatttcggcg cccagctttt ggcatgccag gccgtcttcc ggcgttgagc agtcggtcat
    44821 cgccagtaaa ccgtgatgat gtatacgtgc cagcagcgtt tcaacaggca ccggacgcgg
    44881 gcggtcggtg ccgtcaatgg cgataatgtc cgcgcccgcc tgcgccagcg catcgacatc
    44941 ttcaatatag gccgtgatgc gtaccggaga atcctccaga tcgcgtttca caattccaat
    45001 aatcggcacg ctcaccaccg cacgcgtggc ttgcagattt gccacacctt cgatgcgaat
    45061 ggcaaccgcg cccgcctgct ctgccgctaa tgccatggct gcgacgattt cgggtttatc
    45121 gagcgggctg tcaggaatcg gctggcagga gacaatcagg ccaccgttag cagcgatttt
    45181 ttgatccagt tgtgcaagta acgacataca tcttccctta gcgaaaggcc cggtacatag
    45241 accgggcaac aggattaact tttggttttg actaaatcgt ttttggcgct gccaaatggc
    45301 acggcaccgc taaatggttt gccgtcgata gcgtcatgag tacgcaatgc ttccgggcgc
    45361 agccaacgct gaacgcggga aggcatatcc agaccaatca gcaggatcac cacaaatgtc
    45421 aggctgaacg agagcgatgc cagcgcagta cccagatcca gacgttgagc gatcaacgcg
    45481 ccgatgattg gggccagtgc gccacccaat gcgccaacgt tgtaggtaaa gcccaggccc
    45541 gctgcacgct ggtcggtatc gaaataaccg ccaatcagtt tcggtaagat cccggcgatc
    45601 ccttgtccaa gcatttgctg gaagaacagt aacagaccga gcacccagac gtttgcgccg
    45661 ccaatcgcaa ataccggaat aatcagcagc tgcgaggcca gcaggctaca aacgtacgct
    45721 ttgcgggttc ccagccagtc accgaggaag cctcctacgc agcatcccac ggccgcgcca
    45781 aagccactaa agaacagcac attggcgaca gtatgcgggt tataagccag atcggttttc
    45841 agatacgttg gcagcagcgc ctgaatcggc catgagtaga ggaaagcaaa caacacgacc
    45901 accatcagca ttacgcccgt tggccagcgt ttgcctgtac tctgcaccat aaagctgata
    45961 aagattgcgg cgcataacag cccaagaaca gcgacgatcg cggcattttg caggttaccg
    46021 gcgaagcaga accacagcgc agtagccgcc gccagtgtca ttacgatatt ggcaatgcga
    46081 tgttcaccac ggtagagaat atccaccatt gtgcgtactg gtgctttacc tgcgtgtttc
    46141 tctttccagt cttccgcttc cgggatgttt ttacgcagcc agagagcaaa gatgattggc
    46201 aaaatgccga taaagaacag cgcacgccaa ccccagaccg gaaccaccag gctatagacc
    46261 tgagcggcaa cgacggcccc cacagagaag cctgaaatca aaaaaccact ggctttgtta
    46321 cgcagatgtt ttggccagct ttcaatgaca taggtggcgc tggaaccgta ttcacccgcc
    46381 atccccatgc cgatgaccag acgagcgata aacatggtga tgtagcctgg cgcaaagccg
    46441 caggccagcg tcccggccga gaagagaacg atgctggtga ccattgccag acgacgcccg
    46501 tagcggtcac ccatagcgcc gagcatcagg ccgccgaacc agcgagagat aaaggctgcg
    46561 gagatcagac ttgccgcctg caccgtcgtc agcccgaatt caccctgtac ttcggtgagt
    46621 accagggcga ttaaaacgaa atcaaaaccg tcaagcagat atcccaacca ggcagcggaa
    46681 aatgcgcgcc attgtgcacg gttgagatgg cgataccacg ggatattctg ggttgtagta
    46741 ctcattgtga gtctcccgcg gtgggcgatg cccacacgct ttggtatgaa aattgtaggg
    46801 tacagatgcg tttatttccc ctcacccggt aggggcgagc gaggggaaaa caactcaccc
    46861 gcgctcttgc atcaactgct gggccagcgc cttcagttct ggcagatatt tttcatctac
    46921 cggtccaaac ggtttgcggc acagcggcac agaaacgaca tccatataat gcaggacagt
    46981 tttcaggccg cggaatacgc cagttttgat cagtaaatca atgactttgt tgcactcagt
    47041 ttgcagtttc tgcgcggtct ggatatcgcc ttctttcagc gccttcacga tcccctgata
    47101 tcgccagccc atgatgttgt aagtactgcc gataccacca tcagcgcctg ccagcaagcc
    47161 ggaagcgaag atttcgtcgt aaccgttata gagcaccaga tcaggatgtt cacgacggat
    47221 ctgttccatc tgatagagat cgccagaagt ctgtttcagc gcaccgacgc caggcaacgt
    47281 aaccagggtg ttgatctgat ccagggtcag ttttacccca ctcagggctg gaatgttgta
    47341 cacaaccatc ggcaaaccat ccgctgaatc aataattgcc cgatagtgat cgcagtgttc
    47401 ttcaaagctg aaaggatagt agaacggcgt gacggcggag acggcatcga agccataacg
    47461 tttagccgat gccgcaagtt gttggctttc ggcggtgctg acgcaaccga cgtgggcgat
    47521 gagtttaatc ttacctttcg cctcttcggc gacgatttcc agtacctgtt cacgctcgga
    47581 aaggctttgt acaaaggcct cgccggtcga accacccacg tataaaccgt cgatgccctg
    47641 ctgaatattg aactgaacca ggcgacgcag actcgcttta tccagtgctt gttgttggtc
    47701 aaaaggagtc aggagtgcag ccattacgcc acgtaaattc gttgccataa atacctctga
    47761 agtgatactt gtctgataaa cgatatacct ttatacctgt tataccagat caattaagca
    47821 acaccccata cagaaagctt ataatgcgat ctgcttcact aaagtggcat tatttctttt
    47881 tgttggtggt ctgaccgaaa gcgtgccagg tagcagagac gctgttgaga tgcgattgca
    47941 acgcacgatc ggcttcgtca ggatcatgac ggcggatcgc atcaacgatc gcaatatgct
    48001 gttgatagct aacgttgtta tgctcgtgca gagcctgatc agccacggtt ggacgtgcgg
    48061 cgatcagcca gtcgagcagg gcaacgtgga tcgccatgaa gattgggtta ccagggatct
    48121 ccgccagcac gcggtggaaa tcaacgtctg aacgaatgaa tgccgcgttg ttatccagcg
    48181 actgactgtt gatttccagt gcttttgcca gcaaatcgat ttgctcatcg gtggcatgtt
    48241 cagccgcata gcgcaccaga ctggattcaa agaacagacg taattgttcg aaatgggcaa
    48301 tcccaccggg atgagaaagg aaatctttcg ccatgccgga aaactcaccg atgatagtgt
    48361 ccgcagaagg acgcgagacg cgagcgcgtt cgccgttgtt tatttgcacc agacctttgc
    48421 gttttaacgc tgccagcgct tcgcgcacag aaggacgccc gacgttaaag aacgccatca
    48481 gttcgcgttc agacggtaat tgttcacctt cgccaaattc acgacggcgg atcatctgtt
    48541 ccagctcttc ttccaccatt tcggagagtt ttttacgcgc cagcgggcgg ctacgcaagt
    48601 tgcgaccaat tgcaggtgaa gaatcttcgg tttgcgaatc aaatgcgttc ataaggccca
    48661 ttctgtaagg tcagtgtgat taacatcatc agtgacatcc tatcacagga ttgaaagtag
    48721 gggaaaatgg caggtttttc tctttgtgcc tcatcattac cataattaac ggaataatta
    48781 actattgcga aaaattaatg taacgcagat aaacacatcc cgattgaatt attcataaga
    48841 ctattcacaa gcattatgaa tattatgaat gtgttcttac aaaataatca taagcgcata
    48901 ttttttaatg aaaaatcaca tcgcctacaa ttaaaaacac gacatccgca ccataaatag
    48961 acttgcaaaa aatataacat cattgttttc aatctgccgt ttatgggatt gaccgttttc
    49021 ttttgacacg gagttcaaca atgttcggca taattatatc tgtcatcgta ttaattacga
    49081 tgggctattt gatcctgaaa aactacaaac ctcaggtggt gctggctgcc gcaggtgtct
    49141 tcctgatgat gtgcggagtc tggttagggt tcggtggtgt actcgatccc gccaaaagca
    49201 gcggctactt gatcgtcgac atttataatg aaatcctgcg catgctgtcc aaccgcattg
    49261 ccggattggg gctgtcgatt atggcggtgg gcggttatgc ccgctacatg gagcgcatag
    49321 gagccagtcg cgcgatggtg agcttgctaa gccgcccgtt aaaactcatt cgctcgccgt
    49381 atattattct gtcggcaact tacgtcatcg gccaaatcat ggcgcagttt attaccagcg
    49441 cctccggtct gggtatgttg ctgatggtca ccttatttcc gacgctggtg agtctgggag
    49501 taagtcgcct ctctgcggtg gcggttatcg caaccacgat gtccattgag tgggggattc
    49561 tggaaacgaa ctccattttt gcagcccagg tcgcgggaat gaaaattgcc acatacttct
    49621 tccactacca gcttccggtc gcctcttgcg tcattatctc ggtggcgatc tcccactttt
    49681 tcgtgcaacg cgcttttgac aaaaaagata aaaatatcaa tcacgaacag gcagagcaaa
    49741 aaactctcga taatgtcccg ccgctctatt acgccatttt acctgtgatg ccgttaatcc
    49801 tgatgctcgg ctcgctgttc ctcgcccacg tcgggctgat gaagtcagaa ctgcatctgg
    49861 tggtggtgat gttactaagt ttgacggtga cgatgtttgt tgagttcttc cgcaagcata
    49921 acttgcgcga aacaatggac gatgtgcagg cgttttttga cggcatgggt acgcagtttg
    49981 ccaacgtggt aacgctggtg gtcgcgggtg aaatatttgc gaaaggctta acgacgattg
    50041 gcactgttga tgcggttatc aggggtgcgg agcattctgg tctgggcggt attggcgtga
    50101 tgattattat ggcgctagtc attgccattt gtgccattgt gatgggctct ggcaatgcgc
    50161 cgtttatgtc atttgccagt cttattccga atatcgcagc cggactacat gtaccagcgg
    50221 ttgtaatgat tatgccgatg cattttgcca cgacgctagc gcgcgcggtt tcgccgatta
    50281 ctgcggtggt gaagcggaca gcgatcccca tggcagtcgg tttcgtggtg aatatgattg
    50341 ccacaatcac gctattttat taagtcatta aaaagacaaa acaggccgcc tgggcctgtt
    50401 ttgtattact tcacaacacg taatgccggt cgaccaccgc gtggtggctg cggaggttca
    50461 tcgtcaggat gagtgtcatc atcgtgatct ggcttgtcgc catcaataac cgacataacg
    50521 gtttcgttgt ctgccgatgc ctcttcatca ttcatgatgc tggtatcttc atcgtaggca
    50581 gcttcaggct caaacatcgt gcctgcgcca ttttcacggg cgtagatagc cagcacggca
    50641 gccagcggca cagaaacctg acgcggaatg ccaccaaagc gcgcgttaaa gcgcacctca
    50701 tcattcgcca gttccagatt gccgacagca cgcggcgcaa tgttgagtac gatttgcccg
    50761 tcacgcgcat attccatagg aacctgcacg ccagggagcg tcacatccac caccaggtgc
    50821 ggcgtgagct ggttatccag caaccactca tagaatgcac gcagcagata gggacgacgt
    50881 ggtgttagct gtgacaaatc catacagatt aactccggcc cagacgcatt tcacgttctg
    50941 cttcagttaa agaagcaagg aaagagtcac gctcaaagac gcgggtcata tagcctttca
    51001 gctctttcgc acccgggccg ctgagctcga tacccagttg cggcagacgc cacagcagcg
    51061 gagcaagata gcaatcgacc aggctgaact catcgctcag gaagtacggc ttctgaccga
    51121 agaccggcgc aatcgccagc agttcttcgc gcagttgctt acgtgcggca tctgcttcag
    51181 aagctgaacc gttgatgatg gtgttcatca gcgtgtacca gtctttttcg atgcgatgca
    51241 tgtacagacg gctttcaccg cgagctaccg ggtaaacagg catcagtggc ggatgcggga
    51301 aacgctcatc cagatattcc ataatgatgc gagattccca cagggtcagc tcacgatcca
    51361 ccagggtcgg aacgctctga ttcgggttga ggtcaatcag atcctgaggc ggattgtcct
    51421 tttccacgtg ttcgatctcg aaacttacac ctttctcagc cagcacaatg cggacctgat
    51481 ggctatagat gtcagtagga ccggaaaaca gcgtcattac cgaacgtttg ttggcagcga
    51541 cagccatgaa aacctccagg tatagtcaga atttttactg ctaccagcca ccaggtggcc
    51601 agtcagaagt tgtgttaccc aataaggaac gactctcttt gttcgaaaat caaacaaaaa
    51661 atgagcaata cccgacattt gggcagaaaa ttggatgata gtttaccaga ttttgcgacc
    51721 attgtggtga gtggatgccg gaaatgggga aaaagagatg cgctttagtc tgaaatagtt
    51781 gacttagacc cttattggcg atgtggtttt tgttttgcct gtctttcaga cggcagcaaa
    51841 aagcaacttc ccatttttta cgctgattca gaatttagcc ataaaaaaac ccgccgaagc
    51901 gggttttttc gtaaattgtt ttctgccgga gcagaagcca attaacgttt ggagaactgc
    51961 ggacgacgac gtgctttacg cagaccgact ttcttacgtt caacctgacg agcgtcacga
    52021 gtaacgaagc cagctttacg cagttcagaa cgcagggact cgtcgtattc catcagagcg
    52081 cgggtgatac cgtgacggat cgcaccagcc tgaccagaga taccaccacc tttaacggtg
    52141 atgtacaggt ccagtttctc aaccatgtcg accagttcca gcggctgacg aactaccatg
    52201 cgggcagttt cacgaccgaa gtactgttcc agagaacgtt ggttgattac gattttaccg
    52261 ttgcccggtt tgatgaaaac gcgagctgcg gaatttttgc ggcgaccagt gccgtagtat
    52321 tgattttcag ccattgccta taatcccgat tagatgtcaa gaacttgcgg ttgctgtgcc
    52381 gcgtggttgt gctcgttacc cgcgtaaact ttcagtttac ggaacatagc acgacccagc
    52441 gggccttttg gcaacatgcc tttaaccgcg atttcaatca cacgctcagg acggcgagca
    52501 atcatctctt caaagctcac ttgtttgata ccaccgatgt ggccggtgtg gtgatagtac
    52561 actttgtcag tacgcttgtt gccggttaca gcaactttgt cagcgttcag aacgatgatg
    52621 taatcaccgg tatctacgtg cggagtgtat tccgctttgt gcttaccgcg taggcgacga
    52681 gccagttcag tagccagacg gcccagagtt ttaccggtcg cgtcaacaac ataccagtcg
    52741 cgttttacgg tttctggttt agctgtaaaa gttttcatta aaagcttacc caataaatag
    52801 ttacacgttg gtgaacaccc aaacgtcttc aattgttgag gttcacacga caaagtccgg
    52861 caaacctacc ccttcgaata gcctatgcca gcacacaaaa agttttggga aaaaaacttt
    52921 cttgtaacgt ggggtcgcag gattatagag aagtcggggt caaagatcga cccctttttg
    52981 tgatttgtga caggttttaa cccgccaaat gctcgcgctt cagatactct tcgctttgca
    53041 tctcttgcag acgtgacagg caacgctgga actcaaactt cagccgatcg ccctgataaa
    53101 tttcatacag cggcacttct gcactcacca ctaatttgac atggcgctcg taaaactcat
    53161 ccaccagcgc aataaagcgc cgcgcttcgc tctccatcaa ccgcgtcata actggtacat
    53221 caaacaacat gaccgtatga aagagacgtg agagcgcaat atagtcatgc tgactgcggg
    53281 cgtcgacgca cagcgtagta aaagagaccg ccagcgtctg gttctcgacg cccattgtcg
    53341 ctaatggccg atggttgatt tctaacgtcg gtgaattttc tcgtttcccc cccgccagcg
    53401 ccaaccatag tttatccatt tgcgcccggg tttcatcgtg gagtggcgaa agccacagat
    53461 gcgcctgagt gagtgtacgc agtcgataat caacaccagc gtccacgttc attacatcac
    53521 aatgctgttt aatggcatcg attgcaggca gaaaacgcgc acgttgcaag ccatttcgat
    53581 aaagttcgtc cggcggaata tttgacgtcg ctaccagggt aataccgcgg gcgaacaagg
    53641 ctttcatcag accgccaagt agcatggcat cggtaatatc agaaacaaaa aattcgtcaa
    53701 aacagagcac gtcagtttcg gctttaaagc gatcggcaat aatttccagc ggatcggtct
    53761 gcccctgtaa ggcagttagc tcttcatgca cccgcagcat aaaacggtga aagtgcaggc
    53821 gctgtttccg ctctcccggc aggctttgat agaaaaggtc catcagccag gttttcccgc
    53881 gtcctacacc gccccacata tataagccac gcactggcgt atgctttgtg tcttcgcgtt
    53941 taccccacag cttaccgacc cgcgccatta gcccactcgt cctgggggct ggtggcgtgc
    54001 tattgatgag ttcctgataa ataatttcca ggcggctgac ggcctctttt tgaacgtcgt
    54061 cgggttgatg gctgccttca ttgagcgcct tcaggtattg cgatgttggg gtaacgcttt
    54121 gcatgatctt attgttattc cttgaataat cggtgcgccg ttgttcacgg ttgacgaaaa
    54181 aaaggccgtt ctacactacg cgatatgcag tcgggattcc acttctgtgg aattaacggt
    54241 tatagtggca taatcagccg caggcatgga gcctgaagcc aacaccctac ggaaacaaaa
    54301 gacaacggga gatgttcatg acctgggaat atgcgctaat tgggttagtc gtcggcatca
    54361 ttattggtgc tgtggccatg cgttttggta atcgtaaact acgccagcaa caggcgttgc
    54421 agtacgaact ggaaaagaat aaagctgaac tggacgagta tcgcgaagag ctggttagcc
    54481 actttgcccg cagcgcggaa ttactggata ccatggcgca cgactatcgc cagctgtatc
    54541 agcacatggc gaaaagctcc agcagcctgc tgccggaact gtctgctgaa gcaaacccgt
    54601 tccgtaatcg tctggcagag tctgaagcca gcaacgatca ggcaccggtg cagatgcctc
    54661 gcgactattc tgaaggcgca tccggcctgc tgcgtactgg cgcgaagcgc gactaattta
    54721 tttttcgggc gcagccattg cgccctcctc ttctctccct ccccgactat catttaatct
    54781 ggtgtctcat tgttagccgt ctgaaaattc aataacatca aactgttttg aatctctttt
    54841 cttatcattc aggtacgaga gcaggaataa tgaaaaaaca accccagctg ttgagtgcat
    54901 tagcgttaag tgtcgggtta actctctcgg cgtcatttca ggccgtcgcg tcgattccag
    54961 gccaggttgc cgatcaggcc cctctcccca gtctggctcc aatgctggaa aaagtgcttc
    55021 cggcagtggt gagcgtacgg gtggaaggaa cggccagtca gggacagaaa atcccggaag
    55081 aattcaaaaa gttttttggt gatgatttac cggagcaacc tgcacaaccc ttcgaaggtt
    55141 taggctccgg tgtcatcatc aacgccagta aaggctatgt gctgaccaac aaccatgtga
    55201 ttaatcaggc acagaaaatc agtattcagc tcaatgatgg gcgcgagttt gatgcaaaac
    55261 tgattggtag cgatgaccag agcgatatcg ccctgttaca aattcaaaac ccgagcaaat
    55321 taacgcaaat cgctattgcc gactccgata aattgcgcgt cggtgatttt gccgtagcgg
    55381 tcggtaaccc atttggcctt gggcaaaccg ccacctctgg cattgtttcc gcattaggac
    55441 gcagcgggtt gaatcttgaa ggtctggaaa actttatcca gacagatgct tccattaacc
    55501 gcggtaactc cggcggtgca ctgttaaacc ttaacggtga gttaattggc atcaacactg
    55561 caatccttgc gcctggcggc gggagcgtcg ggattggatt tgccatcccc agtaatatgg
    55621 cgcgaacact ggcgcagcag cttatcgact ttggtgaaat caaacgcggt ttgttaggca
    55681 tcaaaggcac cgagatgagt gccgatatcg ccaaagcctt caaccttgac gtgcagcgtg
    55741 gcgcgtttgt cagcgaagtg ttgccaggtt ctggctcggc gaaagcgggc gtcaaagcgg
    55801 gcgatattat taccagcctc aacggcaaac cgctgaatag ctttgctgag ttgcgctctc
    55861 gtatcgcgac caccgagccg ggcacaaaag tgaaacttgg cctgctgcgt aacggcaaac
    55921 cactggaagt agaagtgacg ctcgatacca gcacctcttc gtcggccagc gctgaaatga
    55981 tcgcgccagc gctggaaggt gcaacgttga gcgatggtca gctaaaagat ggcggcaaag
    56041 gtattaagat cgatgaagtt gtcaaaggaa gcccagctgc tcaggctggc ttgcaaaaag
    56101 acgatgtgat cattggcgtc aaccgcgatc gggtgaactc gattgctgaa atgcgtaaag
    56161 tgctggcggc aaaatcggcc atcatcgccc tgcaaattgt acgcggcaat gaaagcatct
    56221 atctgctgat gcgttaatgt cgtaaaccgg gcatcaggct tacgtgtgat gtccggttaa
    56281 ctcgtggtat gctgctgccg ttcccttttt taatgacgcc tccatcatgt ttgtgaagct
    56341 cttacgttcc gttgcgattg gattaattgt cggcgctatt ctgctggttg ccatgccttc
    56401 gctgcgcagc cttaacccgc tttccactcc gcaatttgac agtaccgatg agacgcctgc
    56461 cagctataat ctggcggttc gccgcgccgc gccagcggtg gttaacgttt acaaccgtgg
    56521 tttgaacacc aactctcaca accagcttga gatccgcacc ctgggatccg gtgtaatcat
    56581 ggatcaacgc ggttatatca tcaccaataa acacgtcatc aacgacgccg atcagatcat
    56641 cgtcgcctta caggatggtc gtgtatttga agcattgctg gtgggatctg actctctaac
    56701 cgatctggcg gtacttaaaa ttaatgccac tggcggttta cctaccattc caatcaatgc
    56761 acgtcgcgta ccgcatattg gcgacgtggt actggcgatc ggtaacccgt acaacctcgg
    56821 gcagaccatt acccagggga ttattagtgc cacgggtcga attggtctga acccgaccgg
    56881 gcggcaaaac ttcctgcaaa ccgatgcttc cattaaccac ggtaactctg gcggcgcgct
    56941 ggtgaactcg ctgggcgaac tgatgggcat taacacgctg tcgtttgata agagtaacga
    57001 tggcgaaacg ccggaaggta tcggctttgc gattcctttc cagttagcaa ccaaaattat
    57061 ggataagctg atccgcgatg gtcgcgtgat ccgcggctac attggcattg gcggacgtga
    57121 gatcgcaccg ctgcacgcac agggcggtgg tatagatcaa ctgcaaggga tcgtggttaa
    57181 tgaagtgtca cctgacggcc cggcggcgaa tgcgggtatt caggtcaacg atctgattat
    57241 ttcggtggat aacaaaccgg ccatctctgc tctggagacg atggatcagg tggcggaaat
    57301 tcgccctggt tcggtgatcc cggttgtagt gatgcgtgat gataagcagt taacgctgca
    57361 ggtcaccatt caggaatatc cggcaaccaa ttaagtcgag tgctcaaaac aaaaaaccgg
    57421 agtctgtg
//
LOCUS       DAZRUF010000003        48527 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZRUF010000003 DAZRUF010000000
VERSION     DAZRUF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.003"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            200..1417
                     /gene="alaA"
                     /locus_tag="ACHXOL_000121"
     CDS             200..1417
                     /gene="alaA"
                     /locus_tag="ACHXOL_000121"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR6807213.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1501..2100
                     /gene="yfbR"
                     /locus_tag="ACHXOL_000122"
     CDS             1501..2100
                     /gene="yfbR"
                     /locus_tag="ACHXOL_000122"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR6807214.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2159..3991)
                     /locus_tag="ACHXOL_000123"
     CDS             complement(2159..3991)
                     /locus_tag="ACHXOL_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR6807215.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4078..4726)
                     /gene="hxpA"
                     /locus_tag="ACHXOL_000124"
                     /pseudo
     CDS             complement(4078..4726)
                     /gene="hxpA"
                     /locus_tag="ACHXOL_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(4737..5231)
                     /locus_tag="ACHXOL_000125"
     CDS             complement(4737..5231)
                     /locus_tag="ACHXOL_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR6807216.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5314..5769)
                     /gene="yfbV"
                     /locus_tag="ACHXOL_000126"
     CDS             complement(5314..5769)
                     /gene="yfbV"
                     /locus_tag="ACHXOL_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR6807217.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6107..7309
                     /gene="ackA"
                     /locus_tag="ACHXOL_000127"
     CDS             6107..7309
                     /gene="ackA"
                     /locus_tag="ACHXOL_000127"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR6807218.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7384..9528
                     /gene="pta"
                     /locus_tag="ACHXOL_000128"
     CDS             7384..9528
                     /gene="pta"
                     /locus_tag="ACHXOL_000128"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR6807219.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9718..11238
                     /gene="yfcC"
                     /locus_tag="ACHXOL_000129"
     CDS             9718..11238
                     /gene="yfcC"
                     /locus_tag="ACHXOL_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR6807220.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11271..11813)
                     /gene="yfcD"
                     /locus_tag="ACHXOL_000130"
     CDS             complement(11271..11813)
                     /gene="yfcD"
                     /locus_tag="ACHXOL_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR6807221.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11871..12422)
                     /gene="yfcE"
                     /locus_tag="ACHXOL_000131"
     CDS             complement(11871..12422)
                     /gene="yfcE"
                     /locus_tag="ACHXOL_000131"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR6807222.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12478..13122)
                     /gene="yfcF"
                     /locus_tag="ACHXOL_000132"
     CDS             complement(12478..13122)
                     /gene="yfcF"
                     /locus_tag="ACHXOL_000132"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR6807223.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13258..13905
                     /gene="yfcG"
                     /locus_tag="ACHXOL_000133"
     CDS             13258..13905
                     /gene="yfcG"
                     /locus_tag="ACHXOL_000133"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR6807224.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13962..14324
                     /gene="folX"
                     /locus_tag="ACHXOL_000134"
     CDS             13962..14324
                     /gene="folX"
                     /locus_tag="ACHXOL_000134"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR6807225.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14345..15238
                     /locus_tag="ACHXOL_000135"
     CDS             14345..15238
                     /locus_tag="ACHXOL_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR6807226.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15286..16176)
                     /gene="rpnB"
                     /locus_tag="ACHXOL_000136"
     CDS             complement(15286..16176)
                     /gene="rpnB"
                     /locus_tag="ACHXOL_000136"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR6807227.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLESNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16373..17146)
                     /gene="hisP"
                     /locus_tag="ACHXOL_000137"
     CDS             complement(16373..17146)
                     /gene="hisP"
                     /locus_tag="ACHXOL_000137"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR6807228.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17154..17870)
                     /locus_tag="ACHXOL_000138"
     CDS             complement(17154..17870)
                     /locus_tag="ACHXOL_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR6807229.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17867..18553)
                     /gene="hisQ"
                     /locus_tag="ACHXOL_000139"
     CDS             complement(17867..18553)
                     /gene="hisQ"
                     /locus_tag="ACHXOL_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR6807230.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18643..19425)
                     /gene="hisJ"
                     /locus_tag="ACHXOL_000140"
     CDS             complement(18643..19425)
                     /gene="hisJ"
                     /locus_tag="ACHXOL_000140"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR6807231.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19646..20428)
                     /gene="argT"
                     /locus_tag="ACHXOL_000141"
     CDS             complement(19646..20428)
                     /gene="argT"
                     /locus_tag="ACHXOL_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR6807232.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20694..21263)
                     /gene="ubiX"
                     /locus_tag="ACHXOL_000142"
     CDS             complement(20694..21263)
                     /gene="ubiX"
                     /locus_tag="ACHXOL_000142"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR6807233.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21358..22875)
                     /gene="purF"
                     /locus_tag="ACHXOL_000143"
     CDS             complement(21358..22875)
                     /gene="purF"
                     /locus_tag="ACHXOL_000143"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR6807234.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22912..23400)
                     /gene="cvpA"
                     /locus_tag="ACHXOL_000144"
     CDS             complement(22912..23400)
                     /gene="cvpA"
                     /locus_tag="ACHXOL_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR6807235.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23659..24321)
                     /gene="dedD"
                     /locus_tag="ACHXOL_000145"
     CDS             complement(23659..24321)
                     /gene="dedD"
                     /locus_tag="ACHXOL_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR6807236.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24311..25579)
                     /gene="folC"
                     /locus_tag="ACHXOL_000146"
     CDS             complement(24311..25579)
                     /gene="folC"
                     /locus_tag="ACHXOL_000146"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR6807237.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVSQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25649..26563)
                     /gene="accD"
                     /locus_tag="ACHXOL_000147"
     CDS             complement(25649..26563)
                     /gene="accD"
                     /locus_tag="ACHXOL_000147"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR6807238.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26719..27378)
                     /locus_tag="ACHXOL_000148"
     CDS             complement(26719..27378)
                     /locus_tag="ACHXOL_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR6807239.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27461..28273)
                     /gene="truA"
                     /locus_tag="ACHXOL_000149"
     CDS             complement(27461..28273)
                     /gene="truA"
                     /locus_tag="ACHXOL_000149"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR6807240.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28273..29286)
                     /locus_tag="ACHXOL_000150"
     CDS             complement(28273..29286)
                     /locus_tag="ACHXOL_000150"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR6807241.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29352..30488)
                     /gene="pdxB"
                     /locus_tag="ACHXOL_000151"
     CDS             complement(29352..30488)
                     /gene="pdxB"
                     /locus_tag="ACHXOL_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR6807242.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30587..31582
                     /gene="flk"
                     /locus_tag="ACHXOL_000152"
     CDS             30587..31582
                     /gene="flk"
                     /locus_tag="ACHXOL_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR6807243.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31579..32757)
                     /locus_tag="ACHXOL_000153"
     CDS             complement(31579..32757)
                     /locus_tag="ACHXOL_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6807244.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33032..34252)
                     /gene="fabB"
                     /locus_tag="ACHXOL_000154"
     CDS             complement(33032..34252)
                     /gene="fabB"
                     /locus_tag="ACHXOL_000154"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR6807245.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34411..36417
                     /gene="mnmC"
                     /locus_tag="ACHXOL_000155"
     CDS             34411..36417
                     /gene="mnmC"
                     /locus_tag="ACHXOL_000155"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR6807246.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36538..36816)
                     /locus_tag="ACHXOL_000156"
     CDS             complement(36538..36816)
                     /locus_tag="ACHXOL_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR6807247.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36850..37398)
                     /gene="epmC"
                     /locus_tag="ACHXOL_000157"
     CDS             complement(36850..37398)
                     /gene="epmC"
                     /locus_tag="ACHXOL_000157"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR6807248.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37398..38207)
                     /locus_tag="ACHXOL_000158"
     CDS             complement(37398..38207)
                     /locus_tag="ACHXOL_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR6807249.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38207..39031)
                     /gene="mepA"
                     /locus_tag="ACHXOL_000159"
     CDS             complement(38207..39031)
                     /gene="mepA"
                     /locus_tag="ACHXOL_000159"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR6807250.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39035..40120)
                     /gene="aroC"
                     /locus_tag="ACHXOL_000160"
     CDS             complement(39035..40120)
                     /gene="aroC"
                     /locus_tag="ACHXOL_000160"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR6807251.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40155..41087)
                     /gene="prmB"
                     /locus_tag="ACHXOL_000161"
     CDS             complement(40155..41087)
                     /gene="prmB"
                     /locus_tag="ACHXOL_000161"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR6807252.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41253..41804
                     /gene="smrB"
                     /locus_tag="ACHXOL_000162"
     CDS             41253..41804
                     /gene="smrB"
                     /locus_tag="ACHXOL_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR6807253.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41926..42798)
                     /locus_tag="ACHXOL_000163"
     CDS             complement(41926..42798)
                     /locus_tag="ACHXOL_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR6807254.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(42785..43309)
                     /locus_tag="ACHXOL_000164"
     CDS             complement(42785..43309)
                     /locus_tag="ACHXOL_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6807255.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43306..43776)
                     /locus_tag="ACHXOL_000165"
     CDS             complement(43306..43776)
                     /locus_tag="ACHXOL_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6807256.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(43773..44321)
                     /locus_tag="ACHXOL_000166"
     CDS             complement(43773..44321)
                     /locus_tag="ACHXOL_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6807257.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44296..45047)
                     /locus_tag="ACHXOL_000167"
                     /pseudo
     CDS             complement(44296..45047)
                     /locus_tag="ACHXOL_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(45067..47709)
                     /gene="yfcU"
                     /locus_tag="ACHXOL_000168"
                     /pseudo
     CDS             complement(45067..47709)
                     /gene="yfcU"
                     /locus_tag="ACHXOL_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(47791..48354)
                     /locus_tag="ACHXOL_000169"
     CDS             complement(47791..48354)
                     /locus_tag="ACHXOL_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6807258.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        12042 a        13049 c        11974 g        11462 t
ORIGIN      
        1 ttcactatat aacattgcat gcaaagcata tacacctcat tattttgtca ttattaagtt
       61 attaacagca caatcgagcc ttcccctctg gcaaaatctt attctgcaga ccttcaaaac
      121 accgtcctgg gggagtacat tgttctaagc tgacttccac ggcagggagt ggcgataaca
      181 gcaaaaaagg tcaagattca tgtcccccat tgaaaaatcc agcaaattag agaatgtctg
      241 ttatgacatc cgtggtccgg tgctgaaaga agcaaaacgc ctggaagaag aaggtaacaa
      301 ggtactgaaa ctgaacatcg gcaacccagc cccattcggt tttgacgcgc cagatgaaat
      361 cctcgttgac gtgatacgca acctgcctac cgctcaaggg tattgcgatt ccaaagggct
      421 ttactccgcg cgtaaagcca tcatgcagca ctaccaggct cgtggcatgc gtgatgttac
      481 cgtggaagat atttacatcg gcaatggtgt atcggagctt atcgttcagg caatgcaggc
      541 attgctgaac agcggggacg aaatgttggt tcctgcacca gattacccac tctggaccgc
      601 ggcggtttcg ctttccagcg gtaaagcggt gcattatctt tgcgatgaat cctctgactg
      661 gttcccggac ctcgatgata ttcgcgctaa aattacgcct cgtacgcgtg ggatcgttat
      721 tatcaaccca aataacccaa ccggcgcggt atattccaaa gagcttttaa tggagattgt
      781 ggagattgca cgtcagcata atctcattat cttcgccgat gaaatttatg acaaaattct
      841 ctacgacgat gctgagcatc actcaattgc gccactggca cctgacctgc tgaccattac
      901 ctttaacgga ctgtcgaaaa cgtaccgcgt tgcaggcttc cgtcaggggt ggatggtgtt
      961 gaacgggccg aaaaaacacg ccaaaggcta catcgaaggt ctggaaatgc tggcttcaat
     1021 gcgcctgtgt gctaacgttc ctgcgcaaca cgccattcag accgcgctgg gtggttatca
     1081 gagcatcagt gaatttatta cccctggcgg tcgtctttat gagcagcgta accgcgcgtg
     1141 ggaactgatc aacgatattc cgggcgtttc ctgcgtgaaa cctcgtggtg cgctgtatat
     1201 gttcccgaaa atcgacgcca aacgctttaa cattcacgac gatcagaaaa tggtgctgga
     1261 tttcctgttg caggaaaaag ttctgttggt gcaagggacg gcattcaact ggccgtggcc
     1321 ggatcacttc cgcattgtca cgctaccgcg tgtcgatgat atcgagctgt ctttgagcaa
     1381 gttcgcgcgt ttcctttctg gttatcatca gctgtaatct taatttcact gccggagatt
     1441 gcatccggca gcgttatccc gccacaatga cctgatgatg tcatcatacg taaggtcact
     1501 atgaaacaga gccatttttt tgcccatctc tcccgcctga aactcattaa ccgctggccg
     1561 ctaatgcgca acgtgcggac ggaaaatgtg tccgaacaca gtttgcaggt agcgatggtc
     1621 gcccatgcgc tggcagctat caaaaatcga aaatttggcg gtaatgtcaa cgccgaacgt
     1681 atcgctttac tggcgatgta ccacgatgcc tcagaagtgc tcaccggcga tctccctact
     1741 ccggtgaaat acttcaattc gcaaatcgct caggagtaca aggctattga aaaaatcgct
     1801 cagcaaaaac tggtcgatat ggttccggaa gagctgcggg atatctttgc gccgttaatt
     1861 gacgagcatg catatagcga tgaagaaaaa tcgctggtga aacaggcaga tgcactgtgt
     1921 gcatatctga aatgtctgga agaactcgcg gccggaaata atgaattctt gctggcaaaa
     1981 acgcgactgg aagcgacgct tgaagcgcgt cgcagccagg agatggacta cttcatggaa
     2041 gtatttgttc ccagcttcca tctttcgctc gatgagatta gccaggattc accgctgtaa
     2101 gcagccggag tctgcgtcgc atcaggcaat aagcgccgga tgcgacatca ggctcttgtc
     2161 aaaacggaaa cagcatcggg atcatcacca cacaaaccgc catcacgata atggtgaacg
     2221 gtacccccaa cttcacaaag tcactgaagc tgtaatttcc cggacctaaa accagtgtgt
     2281 taacaggtga agaaaccggt gtcataaagg cggcggatgc tgccatcgcc acgaccatcg
     2341 cgaatggata aggcgacacc cccatcgttt tggcagcagc cagcgcaatc ggagccatca
     2401 acaccgccgt cgcggtatta gagataaata gcccaataac cgccgacaag acaaacaaac
     2461 accccagcat catatgtggc ccgtaaccac cgccaatgtc catcagccct ttcaccgcca
     2521 gcgcgacacc tcccgttttc tgtaatgcca cagcaaacgg catcatccca acgatcaaaa
     2581 taatgctcgg ccagtgaatg gatttatagg cgctttcagc atctatacag cggaatttcc
     2641 ccatcagcag gcaggcgatg atagcggcaa caggattagg aatttcatct gtcagcatta
     2701 acgccaccat tagcaccaga cagaaaatgg catggggtgc ctggctgtgc gcgggcgatg
     2761 cttcactcac ctcttccggt aagttcagcg ctacgaagtc gcggccctgt ttggccagca
     2821 taccgatcag tttccagtta cccacaacca ggatgatatc gcccagcagc agaggctcat
     2881 ccgccagcga accttccagc gccacgccat tgcgctttag ccccaccaca ttcagtccgt
     2941 agcgggtacg aaaaccaatt tcgcgcaccg atttaccaat cagttctgac tcaggaatta
     3001 atgaaatctc tgccatgccc acatcaaggg cctggtcaga aaaatactcg ccgcgcagta
     3061 ccatcggctc cagcaattgc tcactacaaa attgccggag atcgacatca gccgcagaca
     3121 tatcaataag caaaacgtca cgcgcgcgaa attcagaaac cccattaacg ttcacgataa
     3181 cgcgacgaaa acgccgccag cgttcaacac cgatgacgtt agcgccataa cgctcacgta
     3241 atttgagatc atccagccgt tgaccaatca ttggcgatcc ggggcgaata gccagacgtc
     3301 gcgcacgccc ggtcagtcga tattcacgga taagatcgcg aaaggttcga cgcgtccagc
     3361 cttcgcgctg cggggtctgg gtatcccctt tcagcatgaa acgcatcact aacatataca
     3421 aaatacccag caccagcacg accaggccaa taggtgttac gctaaagaaa ctgaagccgt
     3481 gatagccttc acgcagcaat tcactgttga ctaccaggtt cggcggcgtc gccaccagcg
     3541 tcatcatgcc gcttatcagc ccggcaaaac tcaacggcat catcagacgt gacggcgaag
     3601 tttgcatacg catggcaacg cttaacacca cgggaataaa gatagcgaca acgccggttg
     3661 aactcataaa cgcgccaagc cccgcgacgg tcagcatcaa caaaaccaac attttgattt
     3721 cactgttgcc cgcaactttg accagccatg ttcccattac ggtggcaaca ccggtacgga
     3781 ccaaaccatc gccaataata aacaaggcgg caatcaggac aacgttagga tcagaaaagc
     3841 cggaaaatac ttcggggact gtcagcgttc cgcttaatgc aaacgcgaca ataacaaaca
     3901 aagcgaccgc atccatacgc actctgcccg tcgcaaacaa gacaatggca acggccagta
     3961 atgaaagaac ccaaatcaat tcaccgttca caacttatcc ttgttaattg agggagatgt
     4021 cttgattctg ccataaaaaa gccccgacga gacggggcta aatcatgatc aggtgtttca
     4081 ctgaataata acatcgccat ttggctgttt ggtcacagta atttgctcca gactgtggag
     4141 gaccaaatcg acctcattca ggcgcggggt atctgccgga gcgttaaccg caatgacatg
     4201 acaacccgcc gccaggccag aaagcacgcc agcggagcat cttccaccac cacacactcc
     4261 tgcggcgcaa gccccagcag ctgcgcgcct aacagatacg catcaggttc tggttttccg
     4321 cgcttcactc gctcagcggt tacaaacacc tctggtgcgg gaagcccagc tattttatgg
     4381 cgcgctcgcg ctaccggcat ggagccagaa gtcacaatgg cccacggaat acctgcttta
     4441 ttcaaatgac tgagtaaggc gattgccccc ggaagcgggt aataccttcg gtttccgtgg
     4501 cctcgatgtg ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca
     4561 taaaatggcg cagagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg
     4621 gcgctaaccc atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat
     4681 ccaccagcgt tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag
     4741 gcattgataa tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc
     4801 cacacattaa gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccgtga
     4861 gttccggcgt caaaatgggt atagcgccct tccacattaa ccataaagcg gacataaccg
     4921 aggtaacgtg cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg
     4981 gattgctggt cctgcaaatt agaccaggaa acatgcaacg catgatacat ctccataatg
     5041 tcgatgatag tgcggcaggt ttcttctttc agctcgccaa actcgcgatc caattcacgc
     5101 atctgtaatc cgtaacctcg ctcaataatt gtttgcaggc gacggtaacg ttcagcattt
     5161 gccggatcga gcatagtcat catcttgtac tgattagaca aaataagacg ttgcgcgttg
     5221 gtcatttcca ttgttgactc ctgtatcact ctactacggt gaaaaaaaag aaggctgagt
     5281 atgccttctt ttatatgcgt aatcaggggt caattacaaa tcatcaagga aagttttatc
     5341 cagttgtttg aaggcgcgct taagcgtgtc agctaatgcc tggtaatcag gcttgccttc
     5401 aacgggtgcc aacacctgtc cagactcctg caatttaccg cgaacttcat aaaaccagtt
     5461 aaggattgca gggggtaatg gcgttacaga acgcttgccc agccaccaca atccctgcat
     5521 gggtaaactt aaggcgaaca gtgcagtggc aactgccggc ccaagctgac cgcccagggc
     5581 aatctgccag cagagagtaa atacggcgat cggcggcata aaacggatcg cataacgcgt
     5641 catcttgata acgcgatttt cgacaaagac cggggcaagg cgtttttcca gtggccacgt
     5701 ctttgagtaa tgctgtccgc ggcgaaacaa gctaaaaaaa ttaacagaac gattatccgg
     5761 cgttgacatg cttcacctca acttcacata taaagattca aaaatttgtg caaattcaca
     5821 actcagcggg acaacgttca aaacattttg tcttccatac ccactatcag gtatccttta
     5881 gcagcctgaa ggcctaagta gtacatattc attgagtcgt caaattcata tacattatgc
     5941 cattggctga aaattacgca aaatggcata gactcaagat atttcttcca tcatgcaaaa
     6001 aaaatttgca gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc tacgctctat
     6061 ggctccctga cgttttttta gccacgtatc aattataggt acttccatgt cgagtaagtt
     6121 agtactggtt ctgaactgcg gtagttcttc actgaaattt gccatcatcg atgcagtaaa
     6181 tggtgaagag tacctttctg gtttagccga atgtttccac ctgcctgaag cacgtatcaa
     6241 atggaaaatg gacggcaata aacaggaagc ggctttaggt gcaggcgccg ctcacagcga
     6301 agcgctcaac tttatcgtta atactattct ggcacaaaaa ccagaactgt ctgcgcagct
     6361 gactgctatc ggtcaccgta tcgtacacgg cggcgaaaag tataccagct ccgtagtgat
     6421 cgatgagtct gttattcagg gtatcaaaga tgcagcttct tttgcaccgc tgcacaaccc
     6481 ggctcacctg atcggtatcg aagaagctct gaaatctttc ccacagctga aagacaaaaa
     6541 cgttgctgta tttgacaccg cgttccacca gactatgccg gaagagtctt acctctacgc
     6601 cctgccttac aacctgtaca aagagcacgg catccgtcgt tacggcgcgc acggcaccag
     6661 ccacttctat gtaacccagg aagcggcaaa aatgctgaac aaaccggtag aagaactgaa
     6721 catcatcacc tgccacctgg gcaacggtgg ttccgtttct gctatccgca acggtaaatg
     6781 cgttgacacc tctatgggcc tgaccccgct ggaaggtctg gtcatgggta cccgttctgg
     6841 tgatatcgat ccggcgatca tcttccacct gcacgacacc ctgggcatga gcgttgacgc
     6901 aatcaacaaa ctgctgacca aagagtctgg cctgctgggt ctgaccgaag tgaccagcga
     6961 ctgccgctat gttgaagaca actacgcgac gaaagaagac gcgaagcgcg caatggacgt
     7021 ttactgccac cgcctggcga aatacatcgg tgcctacact gcgctgatgg atggtcgtct
     7081 ggacgctgtt gtattcactg gtggtatcgg tgaaaatgcc gcgatggttc gtgaactgtc
     7141 tctgggcaaa ctgggcgtgc tgggctttga agttgatcat gaacgcaacc tggctgcacg
     7201 tttcggcaaa tctggtttca tcaacaaaga aggtacccgt cctgcggtgg ttatcccaac
     7261 caacgaagaa ctggttatcg cgcaagacgc gagccgcctg actgcctgat ttcacaccgc
     7321 cagctcagct ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga aagaggataa
     7381 accgtgtccc gtattattat gctgatccct accggaacca gcgtcggtct gaccagcgtc
     7441 agccttggcg tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt tttcaaacct
     7501 atcgctcagc cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat cgtgcgtgcg
     7561 aactcttcca ccacgacggc cgctgaaccg ctgaaaatga gctacgttga aggtctgctt
     7621 tccagcaatc agaaagatgt gctgatggaa gagatcgtcg caaactacca cgctaacacc
     7681 aaagacgctg aagtcgttct ggttgaaggt ctggtcccga cacgtaagca ccagtttgcc
     7741 cagtctctga actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt cgttatgtct
     7801 cagggcactg acaccccgga acagctgaaa gagcgtatcg aactgacccg caacagcttc
     7861 ggcggtgcca aaaacaccaa catcaccggc gttatcgtta acaaactgaa cgcaccggtt
     7921 gatgaacagg gtcgtactcg cccggatctg tccgagattt tcgacgactc ttccaaagct
     7981 aaagtaaaca atgttgatcc ggcgaagctg caagaatcca gccctctgcc ggttctcggc
     8041 gctgtgccgt ggagctttga cctgatcgcg actcgtgcga tcgatatggc tcgccacctg
     8101 aatgcgacca tcatcaacga aggcgacatc aatactcgcc gcgttaaatc cgtcactttc
     8161 tgcgcacgca gcattccgca catgctggag cacttccgtg ccggttctct gctggtgact
     8221 tccgcagacc gtcctgacgt gctggtggcc gcttgcctgg cagccatgaa cggcgtagaa
     8281 atcggtgccc tgctgctgac tggcggctac gaaatggacg cgcgcatttc taaactgtgc
     8341 gaacgtgctt tcgctaccgg cctgccggta tttatggtga acaccaacac ctggcagacc
     8401 tctctgagcc tgcagagctt caacctggaa gttccggttg acgatcacga acgtatcgag
     8461 aaagttcagg aatacgttgc taactacatc aacgctgact ggatcgaatc tctgactgcc
     8521 acttctgagc gcagccgtcg tctgtctccg cctgcgttcc gttatcagct gactgaactt
     8581 gcgcgcaaag cgggcaaacg tatcgtactg ccggaaggtg acgaaccgcg taccgttaaa
     8641 gcagccgcta tctgtgctga acgtggtatc gcaacttgcg tactgctggg taatccggca
     8701 gagatcaacc gtgttgcagc gtctcagggt gtagaactgg gtgcagggat tgaaatcgtt
     8761 gatccagaag tggttcgcga aagctatgtt ggtcgtctgg tcgaactgcg taagaacaaa
     8821 ggcatgaccg aaaccgttgc ccgcgaacag ctggaagaca acgtggtgct cggtacgctg
     8881 atgctggaac aggatgaagt tgatggtctg gtttccggtg ctgttcacac taccgcaaac
     8941 accatccgtc cgccgctgca gctgatcaaa actgcaccgg gcagctccct ggtatcttcc
     9001 gtgttcttca tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc gatcaacccg
     9061 gatccgaccg ctgaacagct ggcagaaatc gcgattcagt ccgctgattc cgctgcggcc
     9121 ttcggtatcg aaccgcgcgt tgctatgctc tcctactcca ccggtacttc tggtgcaggt
     9181 agcgacgtag aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg tcctgacctg
     9241 atgatcgacg gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc gaaatccaaa
     9301 gcgccgaact ctccggttgc aggtcgcgct accgtgttta tcttcccgga tctgaacacc
     9361 ggtaacacca cctacaaagc ggtacagcgt tctgccgacc tgatctccat cgggccgatg
     9421 ctgcagggta tgcgcaagcc ggttaacgac ctgtcccgtg gcgcactggt tgacgatatc
     9481 gtctacacca tcgcgctgac tgcgattcag tctgcacagc agcagtaacc tcgtcatcat
     9541 ccgcagcttt gcgctgcgga tatctgaacc ggaaataatc actatttccg gttttttatt
     9601 ctcttaatct gcattaatcc tttctgatta tcttgcttaa ctgcgctgca tcaatgaatt
     9661 gcgccatttc actttacata cttaccactt tgttttgtgc aagggaatat ttgcgctatg
     9721 tccgcaatca ctgaatccaa accaacaaga agatgggcaa tgcccgatac gttggtgatt
     9781 atcttttttg ttgctatttt aaccagcctt gccacctggg tagttccggt ggggatgttt
     9841 gacagtcagg aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa agtcgtagat
     9901 ccacactcat ttcgcattct gactaacgaa gcaggcgaac ctgagtatca ccgcgtacag
     9961 ctgttcacga cgggcgatga acgcccgggc ctgatgaact tcccgtttga aggattaacc
    10021 tcaggatcga aatacgggac agctgttggc atcatcatgt ttatgctggt gattggcggc
    10081 gcgtttggca ttgtgatgcg tacaggaacc attgataacg gtatcctggc gcttattcgc
    10141 catacccgcg ggaatgaaat tctctttatt cctgcgctgt ttattctgtt ttcacttggc
    10201 ggcgcggtat ttggtatggg agaagaggcc gtcgcctttg ccattatcat cgcaccgcta
    10261 atggtccggc tgggctatga cagtattacc accgtcctgg tgacctatat tgccacgcaa
    10321 atcggttttg ccagttcgtg gatgaacccg ttttgtgtgg tcgttgctca ggggattgcc
    10381 ggcgttccgg tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat cgccactatg
    10441 attggcctga tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc tcttctgtca
    10501 cgcgtgcatg agtccgaccg cttctttcgt gaaaagcagg cggatgttga acaacgtccg
    10561 tttacctttg gtgactggct ggtattgatt gtcctgaccg ccgtaatggt ctgggtgatt
    10621 tggggcgtga tcgttaatgc ctggtttatt ccagaaattg ccagccagtt cttcaccatg
    10681 ggtctggtga ttggcatcat cggcgttgtt ttccgcctta acggcatgac ggttaatacc
    10741 atggcttcat cctttaccga aggggcgcga atgatgatcg cccctgccct gctggtgggt
    10801 ttcgccaaag ggattttgct gctggtcggt aatggtgaag cgggtgatgc cagcgtgtta
    10861 aataccatcc tcaacagcat tgccaatgcc attagcggtc tggacaacgc ggtcgcggcc
    10921 tggtttatgt tgctcttcca ggcggtattt aatttcttcg tgacgtccgg ttctggtcag
    10981 gcggcgttaa ccatgccgtt actggcaccg cttggcgatc tggtcggtgt taaccgtcag
    11041 gttaccgtgc tggcattcca gtttggtgat ggcttcagtc acatcattta cccaacctca
    11101 gcttcgttaa tggcaactct cggtgtgtgc agggtggact tccgtaactg gctgaaggtg
    11161 ggcgcgaccc tacttggact gctgtttatt atgtccagcg tcgtagtgat cggcgctcag
    11221 ttgatgggct accactaaaa atgttaagag ccgcaattgc ggctcttttt tcattctgcc
    11281 gtttcagtct ctacggcttc atttttggca ttgcgcttca tccacaacgc cagcgctttc
    11341 agcgagtctg gagtgaactc atcgcagcgt gcggtgattt cttccggcgt cagccagcaa
    11401 acttcactga cttcatcttc ctgtagggcg aagggaccgt gagagacgca gctgaacaac
    11461 gcgccccaga cacggcaatt tttatcttcg aaatagaact gcccgtgctc ggcaaagggg
    11521 acaccggcaa tgcccaactc ttcttccgct tcgcgacgcg cggattccag cagttgctca
    11581 tcggcctgaa ccacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa gtcttttgtc
    11641 tcggtacgac gctggaccag aattttgccc atgccatcat gcacgacgat gtaagttgca
    11701 cgatgacgca gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat gacttcgttc
    11761 tcttcattga caatatccac ccattcagta cttgccaaac gacgctgttc cattatcagg
    11821 aaaccttctt tttctggcgc tcttacggcg cattttgaat tgtgggtaaa ttacggatta
    11881 atcgcgacct gcgcaatgat actttgatca ttgagtgcga ttacactaag tacgtcatta
    11941 tccagcatgc cataactcgc cggattaccg cctttcggaa tactcaccga gccggggttg
    12001 aagtggaaaa tctccccccg ctgttctgcc actggcagat gggtatgacc gtacaccagc
    12061 acatcgttct ggtttaaagc aggtagattt tccgggccaa aaagatgacc atgcgtcaaa
    12121 aacagacgtt gtttttccag taatacctgt tgccacggcg cggttatcgg gaaatgcagc
    12181 agcatttgat ccacttcgct gtcgcagttg ccgcgcacag cgataacctt atgtgccact
    12241 tcattaagcc gttcagcgac tttggctggc gcgtaaccct ccggtaaagc attacgcggg
    12301 ccgtgattca acacgtcgcc aaggatcacc agccactggg caccgctttg ggcaaacaac
    12361 tccagaacac gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc
    12421 atcactcact cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc gttgctatca
    12481 gccagattgc ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct gccactggaa
    12541 tgtcgcataa tccaccaggc gttctggcac ctcatcgcca tgtagtacca ggcggttaat
    12601 cattagcgcc agatcagtat cagcaatgca ccattcacca aataaattcg gctgacccag
    12661 tgctaacaaa tgttctgcca tcgcgaacag tttctctgca ctggcttttc cctcggccgt
    12721 tagtggcgct tttttcgccc ccgcaaagac aacatccgtc ggacgctctt cgcggatggg
    12781 catcagatcg ctgcgcagcc aggcctgaat ccgtcgtgca cgcgcacgat tttctaaatc
    12841 aagcggataa atacgttccc aggtcggtgg cgcaaatcga tcttccagat actccgcaat
    12901 ggcagaagat tcactcaact caaaatcatc gatttgtaat aacggcacac ggcgtgtctg
    12961 accgtaacct tgccacgtcg gcttcaaatg ttcaccgctg tcgaggtcga tggtcttgat
    13021 atgaaacgac aggccttttt cctgcaacgc cacccaggcg gataacacat aaggggagaa
    13081 aaagtgggca tctgaccaaa gcgtgatagc gggtttactc attatgtcct cggcaataaa
    13141 gggggctggc tatcaaacta tagagccttt ttgtcactgt cacgtgacga atactcacgt
    13201 aagtcgccgc gctttctata cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg
    13261 atcgatctct atttcgcccc gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa
    13321 gcagagcttg attatcgctt gattaaggta gacctgggga aaggcggtca gtttcgtccg
    13381 gaatttttgc gcatttcgcc caacaacaaa attccggcaa ttgttgatca ttctcctgcc
    13441 gatggcggcg aaccgctaag cctctttgag tctggtgcca ttttgttgta tctggctgag
    13501 aaaacaggac tctttttgag tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta
    13561 ttctggcagg taggcggact ggggccgatg cttgggcaaa atcatcattt taatcacgca
    13621 gccccccaaa ccattcctta cgctattgaa cgttatcagg ttgaaactca gcgtctttac
    13681 catgtactaa acaagcggct ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt
    13741 gcggatattg cctgctggcc gtgggttaat gcctggactc gccagcgaat tgacctcgca
    13801 atgtatccgg cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc caccgggcag
    13861 gcactgctaa aagcacaact cggtgatgag cgttcggata gttaacagaa acaggttctc
    13921 gtgtattatt tcatcctaag taaaacaacg gagaacctgc aatggcacaa cctgccgcta
    13981 ttattcgtat aaagaacctt cgtttacgta cgtttatcgg aattaaggaa gaagaaatta
    14041 acaaccgtca ggatattgtt atcaatgtga cgatccacta ccccgccgat aaagcgcgca
    14101 ctagcgaaga tatcaacgat gcgctgaatt atcgcaccgt aacgaaaaac attattcagc
    14161 atgtagagaa taaccgtttc tctttgttgg aaaaattaac tcaggatgtg ctcgatatcg
    14221 cacgtgaaca tcactgggtg acgtatgctg aagtggagat cgataaactg cacgcgctgc
    14281 gctacgccga ttcggtatcc atgaccttaa gctggcagcg ttaaccgcca tatcgggagg
    14341 ctgcatgaac atagtgatca ccggagggac gggattaatt ggtcgccatt tgattccgcg
    14401 tttgctggag ctgggccatc aaatcacggt agtgacgcgt aacccacaga aagccagttc
    14461 cgttctcggc cctcgggtga cgctatggca agggctagcc gatcaaagca acctcaacgg
    14521 cgttgatgcg gtaatcaacc tggccggaga accgattgct gataaacgct ggactcacga
    14581 gcaaaaagag cgtctctgcc aaagccgctg gaatatcacg caaaaactgg tcgatttgat
    14641 taatgccagc gacacgccgc cgtcggtact catttccggc tcggcaacgg gctattatgg
    14701 cgacttaggt gaagtggtgg ttaccgaaga ggaaccgccg cataacgaat ttactcataa
    14761 actctgcgcc cgctgggaag agattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg
    14821 cctgctgcga accggcgtgg tactggcacc ggacggcggt attctcggta aaatgctgcc
    14881 accgtttcgt cttggcctgg gcgggccgat tggttccggt cggcagtatc tggcctggat
    14941 tcatattgat gatatggtca acggcattct ctggctgctg gataacgacc tgcgcgggcc
    15001 atttaatatg gtttcgccct acccggtacg caatgaacaa tttgcccatg cgctcggtca
    15061 agcgctgcat cgcccggcca ttttgcgcgt ccccgcaacc gccattcggc tgttaatggg
    15121 cgaatcttca atactggtat taggtggaca acgcgcgctg cctaaacggc tggaagaagc
    15181 gggttttgcg tttcgctggt acgatttaga agaggcgctg gcggatgtcg ttcgctgatg
    15241 tggtttacag caaacatccg ccagttaact cccggtgtta caggattagt ggctttgcgc
    15301 gataagatcg tctggtgaaa gtcgggtcac catcataact aactctctgt ctaaacctct
    15361 atccagcatc tcctgagcaa tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc
    15421 ttcacgtaat ctgtcagcaa tggtcatcag tttctccttt tcttgtggtg cgcgttctgt
    15481 tatctcacca ataaatgcac ggaaacgctg ggcatcccct gtttgtaata cgtaattaaa
    15541 cagggctttt agctgtctgt cattagtgtt ccctgtaact agcagcgaaa caatttggtc
    15601 gactaatccc aacagatcgc gctgacgaat atgtttctga attaactcca acagcgccat
    15661 tttgcggtgt tgcataatct cgtcatccgg caccacggta atatccacca acggaaacgc
    15721 tgatgaatat attttgcggg ctatagcagg ctcggcaaat tcatcaagcc agcagagtga
    15781 ataaggataa ggacttctgc aaccatgata aaacagcatc gggatcacca atggaagctc
    15841 tttatagccc gcatcaagat ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa
    15901 agccattaat tcttccagct tactttggtg ctctatcact acataaatat aacccactcc
    15961 ctcctgcgtt ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat
    16021 aaaactgttt gattctagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg
    16081 aagatgaata tcaataaaat cccgcgcggt gtctggatgg cgtaaaaaag atttaaatac
    16141 cgcatcatgc ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt
    16201 aagatgacgc gacaataacc acgattaaca cccaatgcag caggcagttt ttatctttgg
    16261 acagcgaatt cagaagtatt tttagcggac tgcaactttt acatttcagc ggaagctgac
    16321 tcgcaaattc cccatgaaaa tactccggca gcgttagcca ccggagagga aattatttca
    16381 gcgatccctt aaggaactgt tgcagacgag ggctttgcgg gttgccaaat aactgctccg
    16441 gcgcgccctc ttcttctatt ttcccctgat ggaggaaaat gacgtgagta gaaacatggc
    16501 gagcaaagcc catttcgtga gtcactacca ccatggtttt cccctcttct gccagttgct
    16561 gcataatacg taacacttcg cctaccagtt caggatcgag cgccgaggta ggttcatcaa
    16621 acagtaaaac ttccggttcc atcgccagcg cccgcgcgat agaaacacgc tgttgctgac
    16681 cgccggaaag atgcaccgga tatttcccct gcgcacgttc gtctatcccg actttcgcca
    16741 gatacttcac cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg
    16801 cttccatgac gttttccagc accgtcatat ggctccagag attgaagtgc tggaatacca
    16861 tcgtcaggcg tgtgcgcagt aagcgcagtt gatttttatc ggcgactttg agttgaccgt
    16921 ctttgtcgcg caccagattg atcgtctggc cattgaccac gatcgaccct tcactcggtt
    16981 tttcgaggaa gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga
    17041 tgcttattac atctccggca ttcgcttgca gtgagacccc tttcagcact tcatgttcgc
    17101 cgtagcgttt gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc
    17161 gttgaagaag gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc
    17221 aggacataag agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga
    17281 tacgtggcgg cgttaatatc gcgggctatt ttcagcagat ccggcacagt ggccgtaaac
    17341 gccagcgcag tagagtgcag catcaggatc acttcgttgc tgtatgccgg taacgcaata
    17401 cgcagcgcag aaggcaaaat aatgcaacga tacattttaa aagtcgagaa gccataggct
    17461 ctggcggctt caatttcccc atgcggaacc gaacggattg ccccagcaaa aatctcggta
    17521 gtgtaagcgc aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcggaag
    17581 aaagcgttaa ggaattcggt tcccttaaca atctcaagcg tgtacatgcc ggaatagaac
    17641 accagcaact gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga
    17701 aactggatgt atttattact ggagacacga ccaatcgcca gaaacagcgc caggactccg
    17761 cctatcacta ccgacaaaat aagcagccac agagtgatcg ccacaccagt aaagcgataa
    17821 ccgtcggtcc acagcagcgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc
    17881 tcttcacacc cacggagtag cggcgctcaa ggaacagcag cacaccattg gaaacagtgg
    17941 tgaaaaccag gtaaatcacg ccacagacga tggcgaaata gaacggttcc caggtacttt
    18001 tgcctgccag ttgggtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg
    18061 tagatttgag gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg
    18121 ccggaaacat gatccgccga aacacttgcc cacgagtaaa accgaacgcc gtcgccgcct
    18181 ctatatgtcc tttcggcact gccataaaag caccacggaa cgtttcggta aaataagcac
    18241 cgtagataaa accgagagtg ataataccag cgaccatcgg atcgatatca atctgcccga
    18301 cgcccatcgc ctccgtcacc gtgtttagcg caatctgcaa accgtagaaa atcagcagca
    18361 tcaacactaa gtccggcacg ccacgaatca gcgtggtgta cccttcgaaa atcagccccg
    18421 aaagccgatt ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca
    18481 ctacagagct gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa
    18541 acccatacaa catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct
    18601 ccggttaccc gaagggagcg ggctttcttt caccgatggc gattagccac cataaacatc
    18661 aaaatcgaag tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa
    18721 ggctttgttc agtgcttcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc
    18781 tacgccaaac agtttttcat ctttaacaga cgggccaccg aatttgtaat ctttaccgac
    18841 aggttgtttg aggaaacctt cgctggcagc gacctcatcc tggaacgcgg catcaatacg
    18901 tccggcagtc aggtcagaat aaatgttgtc ctgcccctga tacgagacga tttcaatgcc
    18961 ttttggtgcc caatgttcat taccgaacgt ctcctgggtg gtgccctgca atacgcctac
    19021 ccgtttgcct ttcagcgact cgactgtcgg ctgaatgtca gaatttttcg ccaccaccaa
    19081 acgagaatcg gcagcgtaca gtttgtcggt gaaggctatt tcttgctgac gtttttccgt
    19141 aatggaaagc gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc
    19201 atccagcgga ttttcgacaa acgtacattg cgtattgatg cgtttgcata attcctttgc
    19261 cagatcgata tcgaagccaa ccagttcgcc ttgtgaattt tttgattcaa atggcgcata
    19321 ggtcgggtcg gtaccgatgc ggatgttttg cggaatcgca gcaaatgccg cagttgcgct
    19381 ggagaaggcc agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga
    19441 cagtcttttt atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc
    19501 atcaaggcaa aatattccgc cccgttagac gtattatttt tcattaaaaa tgcttaagta
    19561 tgcatttcaa tgcaccacga tagtgcaaca aacctggcgt gcaccatgat gatcattcca
    19621 tcaggtacag cttcccagcg acgtatcagt caccgtagac attaaagtcg aaatactttt
    19681 tcgccatctt gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag
    19741 ccgtcagttc agcatcatct ttacgtagcc ctacaccggt gccatcaccg aagtattttt
    19801 tgtcttttac tgatgagcca gcaaaggcga aagctttacc agcaggttgc ttgaggaatc
    19861 cttcgctggc agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg
    19921 aatagaccaa atcctggttg gcataggcca ccacatccac gcctttgcta cgccaggtct
    19981 cgttagcgta agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg
    20041 aatccagcgt tggctgaatc ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt
    20101 acagcttgtc ggagaaggca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa
    20161 taatggcgtc gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa
    20221 cccaggtaca tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc
    20281 caacaaaatc acctttagca tctttcgacg agaacggcgc ataggtggta tcggttccga
    20341 tacgtaccgt ctccggtagc gccgcatagc tggaagccgc tgcggagaga ccgactaaca
    20401 aagacagagc gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat
    20461 gttgtgtatt gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catcttgacg
    20521 acaacagtaa cattcaacgt taaatatgtt aataagacgt tgcattattg tcctgaagtt
    20581 gaagatagca ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg
    20641 tcgaggattt gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg
    20701 ccctgccagc gggcaaagag atcttcagga agggttattg caaactggtc aagaacacga
    20761 ttaaccgtct gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaacg
    20821 ggaggcataa tcaccgcacc gatttctgcc gcctgagtca ttaaacgcag atggcctaag
    20881 tgcaatggtg tttcacgcac gcagagcacc aacgggcgac gctctttcag caccacatct
    20941 gccgcacggg tcagtaagcc atcagtatag ctatggacaa tgccggaaag ggttttgatt
    21001 gaacagggta aaatcaccat ccccagcgtc tggaaagaac cggaagagat gctggcggaa
    21061 atatcgcgcg catcgtgcgt gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc
    21121 gtttcgaggg ataaggtctg gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct
    21181 gtgacatcgc gcagaacctg taataagcgc acgccataaa tcgcgccgct ggcaccgctg
    21241 atgcctacaa tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc
    21301 agtatttcgg cgggagttgc aagtcagggt gccagaccgg caccctcagc gaaggcatca
    21361 tccttcgtta tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc
    21421 atcattacgt aacgtatcga ggaaatcgag gtagccctga tcaacatctt tggtgacgta
    21481 gacgccgttg aacaccgagc attcaaactg ctggatatcc ggattttcag cgcgaacggc
    21541 gtcgatcaga tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat
    21601 ttcatcaact tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata
    21661 aacgttcggg aagcgaattt ccggtgccgc agaagcgagg tacactttct tcgctccggc
    21721 ttcgcgtgcc atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac
    21781 cagcaggacg tttttatcgc ggaactcggc gcggttggca ttcagtttac ggcgcacgga
    21841 cttacgacgc agctgctggc ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac
    21901 gaagccctgg cggtacggtt tgcccagaat acgagcaatt tccagcgcga tatcacacga
    21961 ggtttctggg atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt
    22021 ctcgcccagt ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgatgaa
    22081 cgagtccggg cgagcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc
    22141 agcacattga cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc
    22201 gacgtcacgc aggaaatcaa agcccagcgt atcgagcgct acgctttcgg aagcgaccat
    22261 atattctgta cggttctcgt caatatcacg ttttcccagt accagcggac gaatcccgtt
    22321 tggatcgcgg aaagcaacca taccgtggcc gataatcatc gccacacagg catatgcgcc
    22381 gcggattaag cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta
    22441 gtggcggaag ttgtccagct cgctagcgaa gatattaagc agaatttccg agtcggaagt
    22501 ggtgttgatg tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt
    22561 cagattgccg ttgtgggcaa gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc
    22621 ttcagaggcg ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc
    22681 ctgcaaacgc tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg
    22741 caaacggaag cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg
    22801 ctgaagcacc gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc
    22861 gacaataccg cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct
    22921 gggcaagaaa cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa
    22981 ctgcgggatc agctgtgatt tgctccagtc ttcgcttttc gacaccccgg taaaggagtc
    23041 gagaaagaag agaatggcgg caacaatcaa cacaccgcgc aacgcaccga aacagacgcc
    23101 cagcacccga tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt
    23161 cacgatagca ccaacaatca gggtagcgat aaacagtacc gcgatggcaa ttccatttcg
    23221 aaccagttcg tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc
    23281 aacaaagaaa gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgcggat
    23341 caggctaacc agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat
    23401 atgtgtccca cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga
    23461 aaacgtttgc gtagggattt ccttcccacg catcaataaa aatggcgctg aaaaaatatt
    23521 caacgccatc gactttttat gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta
    23581 aacgccttat ccgacctacg gttctaacac tgcccaggcc tgataagacg cgccagcgtc
    23641 gcatcaggca agactgtatt aattcggcgt atagcccatt accacgccac ttaagccaga
    23701 aagttgcttc aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac
    23761 cagaatacgg gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc
    23821 ggcaccgcgc agcttaccga caatctcatt cactttatcg gcatttttca gcgcacccag
    23881 ttgcacaaca taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc
    23941 tggcttcggc gcaggtggtg cttcaacctt cggtttaggc ggctccaccg gtttcggctt
    24001 cggtggggcg accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc
    24061 cggatcgagc gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg
    24121 cggctgcgtc ggcaacgcct gggtggcggc tggcatcata tcaggctcat cgcgatcgcc
    24181 cgcttttgga accagcggga tagccgcaaa ctcatcctga taatgttttt tctgcccgtc
    24241 cagcagccct ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg
    24301 attctgaaac ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga
    24361 ccgtgtggaa agaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg
    24421 catcccatgc ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt
    24481 gttctgccgt ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc
    24541 ttttcaacca ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca
    24601 gcacgcgccc gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg
    24661 gattatgcgc gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg
    24721 gcaaaattgc gctggcaatc ccgtcgcgaa tggcattttc actgacttcc agcccgctgg
    24781 cacgcagtgc cgccagcgct gttgcggcat tcggttgcga gacaagcggc aacggcagat
    24841 tttccagcgt gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc
    24901 actcaacgcc ccgacgttgt aacagtgcac ctttttcctg cgccacatca gcaatggtag
    24961 aaggcatttc cggctcaccg acaattgccg gttttgcgct gcgaaagatg cctgctttct
    25021 cgcgaccaat actttcgcga tctggtccca gccagtcggt atgatccagc gcaatgctgg
    25081 ttactacagc cacatcggca tcgacaatat tggttgcgtc cagacgaccg cccagcccta
    25141 cttccagaat cactacgtca agttgtgcct gtttgaacag ccacaacgcc gacagcgtac
    25201 cgtactcgaa ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg
    25261 cggtgtgggc cgattccggc aattcctgcc cctgcacacg tacgcgctcg gtataacgca
    25321 ccagatgcgg tgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca
    25381 gcgtacggca ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg
    25441 gtttcaggac gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag
    25501 ttttactgtg caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag
    25561 gagtgcgttt gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca
    25621 gagccagtgg ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg
    25681 gtaccactac gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg
    25741 ccagaatgct cgccagtttc aggcgcattt ccggacgatg gacgatcatg tcgatggcgc
    25801 ctttctcgat caggaattca ctgcgctgga atcctggcgg cagtttttcg cgaacggtct
    25861 gttcgataac acgcggaccg gcaaagccaa ttaacgcttt cggttcagcg atgttgagat
    25921 cgcccagcat ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga
    25981 tgtacggcaa gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca
    26041 tcagcgacat cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg
    26101 ggcagttatc ttccagcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc
    26161 ccattgaacc gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca
    26221 gagtgccttt catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagaagcca
    26281 gacggtcttt atacttcttg gagtcacgaa acttcagcac atctttcggc tcaagctcgc
    26341 tacccagctc cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca
    26401 tacgcatgtg atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt
    26461 ataaaacctg accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc
    26521 gggtgggagt aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct
    26581 gaacctggtt cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac
    26641 agaccatgaa tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc
    26701 gaaccgcgga gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacgaataaa
    26761 ttcgaaaacc ccaggcaata ttgaaacaaa aataatgcca acaatcatca gcttaaggtt
    26821 gctctgaatg aaggggagcg tgccgaagaa ataacccgcg taggtaaaaa tcagtaccca
    26881 caacagtgcg ccgatcacgt tataagcggc gaaatgacgg tacgacatgt ggcccattcc
    26941 cgcaacaaac ggcgcgaacg ttctgacgat cggtacaaaa cgggcgagaa taatcgtttt
    27001 gccgccatgt ctctcgtaaa actgatgagt cttatcaaga taactgcgac gaaagatttt
    27061 cgaatccggg ttactgaaca gtttgtcacc aaataaccgt ccaatggtat agtttactgc
    27121 atcaccgaca atagcggcaa tcaacataag caccaccatc atatggacat tgagatcgtt
    27181 agtttccagc gacgccaatg caccagcaac aaacagcagc gaatcaccag gcaggaatgg
    27241 cgttaccacc agccccgttt cgcagaacag aatcaaaaac agaatggcat aaacccagac
    27301 gccgtactcc gcgaccagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag
    27361 gaaataaatc aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact
    27421 acgttggcta cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg
    27481 ggcccattgg cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca
    27541 gcccttccgc ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg
    27601 ctatccatct ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc
    27661 tgaccatatg atgtacaaag gcattcgctt tgatatctac caccacataa ggaccgtgac
    27721 gcgtgacatt aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac
    27781 ggaacgaggt gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag
    27841 cgtccagcgg ttcgtaaaaa tgggttaccc ctttactcaa taccgccggg cgcagccgat
    27901 gattgtagat gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat
    27961 caggtacagc tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca
    28021 aggtccacgc cgcgtctttg cgctgcgcgg ttgtttcgaa atgcaccacc tgcccggtac
    28081 cgtgtacccc tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct
    28141 gggagagcgc cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc
    28201 cgtaatactt actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcagttgtt
    28261 gctggtcgga cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat
    28321 cagcgcgccg ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat
    28381 accgtagtca ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcccctac
    28441 ctgagtcggg aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc
    28501 acgcgcttct tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc
    28561 gtagaatacc ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat
    28621 tttgcgtact tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa
    28681 caacggcagc atattgaacg ccagttgacg accgaagaaa tcttcttcgt caatcggaat
    28741 gccgttgagc aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc
    28801 ggaggctgaa atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg
    28861 tttcagtgcc gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt
    28921 gcggtaatcg gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc
    28981 gagagcaaac agtccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac
    29041 ccaggcagcg gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc
    29101 gaattcagcg gcatcctgca cagtgattgt tttaccacca aagcgcagtt gttcgcctgc
    29161 gctttcgtta cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt
    29221 ttcaagcagg gcttcgccca cagcgccagt tgcgcccagg acggcaatgt tccagccttc
    29281 agacatggtg gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca
    29341 tgaagaagag attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat
    29401 gatgccgcac tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga
    29461 tagtttttgc gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg
    29521 tcatcgcggc gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc
    29581 gggccatgca gcgtaatgcg accaaactct ggcgcgggca gtaatgtatc cagcgcaacg
    29641 tgctgttcat gcccaataaa cttgctatag gcttcaaaca cttgcgtagt accgcgtgct
    29701 ttaccttcca gggtataacc tgcgatatgc ggcgtgccga tatccacttt tgtcagcaac
    29761 tccacgttaa gttccggttc gccttcccag acatccagca ctacgcttaa cttctggcct
    29821 tcattcaggc aggtcagcaa cgcagtatta tcgacgactg cgccacggca ggcgttaatc
    29881 agaatcgctc ccggcttcag gctacggatc agtttttcat ccgccagatg cagcgttttg
    29941 tacggcccat ctttgaagag tggcgtatgg aaagtcagaa tatccgcatg ctgaactaat
    30001 tcatccagcg agcggaaatc accctcatcc ccacggtcag cgcgaggcgg atcgcaaagc
    30061 aatgttttaa tccctaacgc ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg
    30121 cccacgatcc ccaccgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagt
    30181 agtgaggaga aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa
    30241 ccaattcccg cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtg
    30301 ccaacaaatt taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc
    30361 accatcagcg catccgcgtc tgccagttga gcgacgggga ttggacgccc gggaaccgcg
    30421 gtcacctcac ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg
    30481 attttcacgt ttgtgttacc tgtatgagac gagagttaac cggacaagtg tgccataatc
    30541 tcgcggccag gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat
    30601 ttccggccct cctcctgggc aaccaccagg tcagggagat aacctgccgt ctggcgcggg
    30661 caatcagcct ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac
    30721 ctcactgacg caacaacaaa gagcagaact gtgggcgggt atcaggcacg atattggtct
    30781 gtcgggagat tcaccgctgc tttcgcgtca cttccctgcc gctgagcata atctggcgca
    30841 acgtctgctg gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga
    30901 gtatttacgt ctggggaata atcgtcaggc ggtcacggat tatatccgtc ataactttgg
    30961 tcagacgccg ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca
    31021 ggaagggaag atggttattc cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt
    31081 accggcggag cacaatgcgc taaaacagct ggtgaccaaa cttgcggcgg caacggggga
    31141 acccagcaaa cagatctggc aatcgatgct ggaactttcc ggggtgaacg atggcgagtt
    31201 aattccagcg aaactgttta accatctggt gacctggttg caggcgcgcc agacgttaag
    31261 ccagcaaaat acgccgacgc tggaatcact acagatggcg ctaaaacaac ctttagatgc
    31321 cagtgaactg gcagcgttat cggcgtatat ccagcaaaaa tatggtcttt ctgcgcaatc
    31381 atcgctttct tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa
    31441 agggattgat ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac
    31501 gttgcaaaat atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat
    31561 gctggtctgg ctggttcgtt agccccgacg aaatgacagt atcgtgacaa taattcccag
    31621 caccgcagag atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat
    31681 tcccgccagc ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag
    31741 tgcggtgccg cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa
    31801 gataagcgaa catccggctc cggttaacgc cgcgcccgct aatgcgaccc acgcacctgg
    31861 ggcttgccag agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt
    31921 cacgccgcca aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc
    31981 gccaaactcg gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga
    32041 aacgaaagtc ccgataaccg caaaaccaac gccttgtagt gccagcccta accctggttt
    32101 ccagataaac ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt
    32161 gcgcactgtg ccgttacagg cccacgccag taagggtaat accattgtgg tgatcgccag
    32221 tgcggcaaaa ccgtaatggc tatgaatcaa caggccaagc ggagcaccaa cagcgagggc
    32281 accgtaaatc gccattccat tccatgacat cactttgcca gagtgttttg gccctacgat
    32341 gcctaacccc caggtcagag cgcctgtcag taactggctt tcaccaaacc caagaatcaa
    32401 acgcccgatg accaacaggg caaatttgaa cggtgcggag acaggcaaaa tcgccgccag
    32461 cagcaacgcg ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc
    32521 gccatattga tcggccagtc gcccggcata gccacgcgtc agcaccgtag ccagaaactg
    32581 aatcccgacg gcaatgccga ccatggtatt gccatagccc agttcatgat gaacaaacag
    32641 cgggataacc ggcaacggca gccctacggt catgtaggtg agaaaaaccg caaaagcgat
    32701 gcggaagagc gaaaaattgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca
    32761 ttactccaga atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg
    32821 ttacggtgcg ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg
    32881 gaagtttgcc tggaactggt ggcgattgtc aatgatgtga aaaagggaac catcaggttc
    32941 ccttttgcgt tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg
    33001 tcttatccga cctactccgg cctactgcga attaatcttt cagcttgcgc attaccagcg
    33061 tggcgttggt gccgccgaag ccgaagctgt tagacataac agtggtcagt tcgcgatcgg
    33121 tcgtttcggt cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc
    33181 tcggggcgat aaagccgtgt tccagcatca gcagagagta gatagcttcc tgcacacccg
    33241 cagcgcccag tgagtgaccg gtcatggctt tggttgcaga aatcgccggg ctcttatcgc
    33301 cgaacacttc acggatagct gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt
    33361 gagagttcag gtaatcgatt ggggtatcaa cgccatgcat cgccatctgc atacaacgta
    33421 ctgcgccttc gccagacgga gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa
    33481 cgatttcagc atagatgtga gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta
    33541 ccataccgcc gccgccagcg ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg
    33601 ctttttccgg ggtgtcgttg tatttagtag acagcgcacc catcgcgtcg aattcgcagg
    33661 ccatttccca gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt
    33721 ggatctgctc tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg
    33781 agtagttaac gccatgaatt ttaaacggcg tggcgaggca ggcagaaacg ccggatgcca
    33841 tcgctttggt gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt
    33901 cagcgccgaa tacctggaaa cgcggggaac cgccgccaga acctgcaatc aggccaacgc
    33961 gcgggttatt ctggtaagct tccggagaaa ggcccgcatc tgcgattgcc tgctccatag
    34021 aaaggaatgc ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc
    34081 cagtggtatc cagttttacg ttgccccaga cgtggctacg catgccggaa tccttcagct
    34141 cctgagagaa agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt
    34201 tattaccgat gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc
    34261 tctgtaagtc gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa
    34321 caagtccgat cagccattta atagagaaat ttgcgcagcc ttacacacat cgctaagatc
    34381 gagccaccgc ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac
    34441 ctcgaattta atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc
    34501 aacgataacg ggctggaaga gacgcgttat gtttttctgg gaggcaacca attagaggta
    34561 cgctttcctg agcatccaca tcctctgttt gtggtagcag agagcggctt cggcaccgga
    34621 ttaaacttcc tgacgctatg gcaggcattt gatcagtttc gcgaagcgca tccgcaagcg
    34681 caattacaac gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta
    34741 gccttagcgc atcaacactg gccggaactg gctccgtggg cagaacaact tcaggcgcag
    34801 tggccaatgc ccttgcccgg ttgccatcgt ttattgctca atgaaggccg cgtgacgctg
    34861 gatttatggt ttggcgatat taacgaactg accagccaac tggacgattc gctaaatcaa
    34921 aaagtagatg cctggtttct ggacggcttt gcgccagcta aaaacccgga tatgtggacg
    34981 caaaatctgt ttaacgccat ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt
    35041 acgtctgccg gttttgtccg ccgcggtttg caggaagccg gattcacgat gcaaaaacgt
    35101 aagggctttg ggcgcaaacg ggaaatgctt tgtggggtga tggaacagac attaccactc
    35161 ccctgctcca cgccgtggtt taatcgtacg ggcagcagca aacgggaagt agcgattatc
    35221 ggcggtggta ttgccagcgc gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta
    35281 gcgctttatt gcgcggatga agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg
    35341 ctgtatccgt tattaagcaa acacgatgag gcgctaaacc gctttttctc taatgcgttt
    35401 acttttgctc gtcggtttta cgaccaatta cccgttaaat ttgatcatga ctggtgcggc
    35461 gtcacgcagt taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg
    35521 gatttacccg cagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc
    35581 gttgcgacaa attgcagcgg cattacttat ccgcaaggtg gttggctgtg cccagcagaa
    35641 ctgacccgta atgtgctgga actggcgcaa cagcagggtt tgcagattta ttatcaatat
    35701 cagttacaga atttatcccg taaggatgac tgttggttgt tgaattttgc aggagatcag
    35761 caagcaacac acagcgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa
    35821 acgtcgactc tcccggtgta ttcggttgcc gggcaggtca gccatattcc gacaacgccg
    35881 gaactggcag agctgaagca ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg
    35941 gcgaatcaac atcattgtat tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac
    36001 agtgaggacg atcagcagca gaatcgccag cggttgattg attgtttccc gcaggcacag
    36061 tgggcaaaag aggttgatgt cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc
    36121 cgcgatcatc tgccaatggt aggcaatgtt cccgattatg aggcaacact cgtggaatat
    36181 gcgtcgttgg cggagcagaa agatgaggcg gtaagcgcgc cggtttttga cgatctcttt
    36241 atgtttgcgg ctttaggttc tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg
    36301 gcggcgcaga tgagcgacga accgattccg atggatgcca gtacgctgac ggcgttaaac
    36361 ccgaatcggt tatgggtgcg gaaattgttg aagggtaaag cggttaaggc ggggtaatct
    36421 gctctcgcag tgtttgccgg atgcggcgtg aacgccttat ccgacctacg tgtgacgtgt
    36481 aggcctgata agacgcgata agcgtcgcat caggcatggt gctccagacg ccgcaactta
    36541 ctgctgtgac gcctgctgaa acaggttttc ccacatatcg gtcaccagcg cctggtcacg
    36601 cggcgacaat tcaccggcac caatggcttt ttccagactc tggctaacgg tcgtatgcac
    36661 cgcctgagcg gagtggtcat caccactttc cagttctgcg atggctaatg tcaggtggcc
    36721 acgcaaatac ccactggcaa acaactcatc atcactggca tggtcaacca tgccgtcgat
    36781 taatgccaga atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg
    36841 gcaccggctt cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt
    36901 gtgataataa ttttgtagtg ctttaatgaa gcgtgccgga cgctcgggaa tgccatttgt
    36961 cagataatcc atcacctgcg catgtacccg ccgttgaaac acaacacgat ccggttcgaa
    37021 gtcgccttcc agattgtcgc aactgacgtt aaacggatat cccgccgcca cacagaacaa
    37081 ccagtcgagt gcctgcggct tcacttcaac atcttcaaac tggctttgcg tttgggcatc
    37141 acgcccatcc gggcaatacc agtagccgaa atcaaccagc tcacggcgcg cttttccggc
    37201 aatacaccag tgcgaaatct catgaattgc gctggcataa aagccgtggg caaagacaat
    37261 tcggttatac ggtacttccg catcagcagg aagatagatc ggttcgtcgt cgcctttaat
    37321 cagacgggta ttaaattcat cggcaaagca gctattaaaa atttcaatca actgctcata
    37381 gtggtgtgta ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccgtggcta
    37441 tcgtaaagta atttggcact catcaccgcc gagacgataa caatcatcgg gcggatcagc
    37501 ttttgtcctt tgctcaacac cagccgtgaa cccatgcgcg cgccgaggaa ctgccccacc
    37561 agcatgacaa agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagcagaccg
    37621 ccaatgtttg acgttgcgtt gagtaatttg gcgtgagccg tggctttggc gaggttgaat
    37681 ccgcacagcg taacgaaggc cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg
    37741 tcataaaaac cgacgcagcc accggcaatt aacgcgaagg gtaaaccgta catccggcgc
    37801 tggcgatctt cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc
    37861 agaatgggca aaatctgccg taagacatca gcctgaacgt actgtaccaa cagtgcgccg
    37921 ctcattgagc cgacaaaggt catggcaata ttgagtttct gatcgcttaa actaaccact
    37981 ttgcggcgaa taaagtagat agtggcggaa atagagccgc cgcaggcttg cagtttattg
    38041 gttgccagcg cgttagcggg agacattccc gcagccatca atgccggaat ggtgagtaac
    38101 ccaccgccac cggcaatcga gtcgataaat cccgccagca tggcgacaaa aaagagaact
    38161 cccagcaaca gcggggaaac cataaacagg ctattaaacg tttccattag atcacgtgct
    38221 catccagtag cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg
    38281 ttgttcccgg ttttagaggt gcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg
    38341 atggcggtaa aggttgatct tcacactcca gactatcggc agggcaacgt aatcgtacat
    38401 gcatatgcgc gcgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc
    38461 ccgcatcaag gcaaagttgt tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt
    38521 tgtcctgggc agcgagtttg atcaagctga aaatttctgg cttccacagc gtggagacaa
    38581 cgtgtttacc gtcgcgggaa actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg
    38641 tccagcgagt tttcggcagt tgcagaaaga tatcgacatc cagtccggtc tggtggctgg
    38701 catgaccgcc gttgaaacgc ccaccagcgg gcatccccat atcgccaatc agcaccgtac
    38761 ccatgcccag attgctcacc tggctactca gacgctggat aaacatcacc agatccgggt
    38821 gaccgaaata gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca
    38881 acgtgtcagc gccgacaata cagccattag aaaaactgcc tatcgattgt gcgctacccg
    38941 gcacaggttg ggttattttt tgccacggcg tcgctgccag gctagcgcta ctggcaagca
    39001 gagccagcag cgcaatcgcg gttttgttca ttttttacca gcgtggaata tcagtcttca
    39061 catcggcatt ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg
    39121 cttcagcgat cggcactgca cggatcccga cacagggatc gtgacggcct ttggtgatca
    39181 tctcaacttc ttcgccaaag cggttaatag tgcgccccgg cacggtaatg ctggaggtcg
    39241 gtttcagcgc catatgagca atgatttgct gcccgctgct gataccgccg agaatgccgc
    39301 ccgcatggtt gctctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca
    39361 gcgccactac gtcaaaaccg tcgccaattt ccacgccttt caccgcgttg atgctcatca
    39421 gcgcatgggc gatgtcggca tccaggcggt caaagaccgg ctcgccaagt ccggcgggga
    39481 caccactggc aacaacggtg actttcgcgc cgatggagtc gccctctttt ttcagcgcgc
    39541 gcatcagttc atctaacgcg tcgattttgt ccgggtccgg gcagaagaac ggattttgtt
    39601 caaccagcga ccagtctttg atttccagcg gaatgtcgcc catctgggtc aagcagccgc
    39661 ggatttcaat accaaatttc tcggcgagat attttttggc aatcgcccct gccgccacgc
    39721 gcatggcggt ttcgcgggcg gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt
    39781 tttgttcgta ggtgtaatca gcatggcctg ggcggaaaac gtctttaatt gcgctgtaat
    39841 cctgagagcg ctggtcagtg ttttcgatca acaagccaat gctggtgcca gtagtaacgc
    39901 cttcaaaaac accggagaga attttgacct gatccggctc gcggcgctgg gtggtatagc
    39961 gcgatgtccc agggcgacga cggtcgaggt catgttgcag gtccgcttcc gtcagcggaa
    40021 tgcctggcgg aacaccatcg acgatgcagc cgagcgccag cccatgcgat tcgccgaagg
    40081 tggttacgcg aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt
    40141 gtttgcgtgt ttacttaatc tttataaatc gcgaaatatt cacgggccgc cagcagctgc
    40201 tctttggtga gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga
    40261 acatccggat attgttccat aagatgtacc atgctgttgc cgacttcaca aatcaacacg
    40321 ccatcatcag caaggtaatc tgccgcgttt ccaagaatgc gacgcgtcag tttaaggccg
    40381 tcggtgccag atgccaggcc cagttccggc tcgtgacggt attcgtttgg caggtcggac
    40441 atatcttccg cgtcgacata cggcgggtta gtgacaatca gatcgtactg cactttcggc
    40501 aaatcacgga acagatcgga acgaatcgga atgacgttgt ggatcagacc gtgttcttcg
    40561 atgttctgtt cggcaaccgc cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct
    40621 tccgggaagg cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa
    40681 atatgctgcg gttgcttgct aatgagtccg gcaaatttat tgttgatcag ttcaccaatc
    40741 ggcgagcgcg gcaccagcac gcgttcatcg acgtaaaatt catgaccgca gaaccaggct
    40801 ttgttggtca ggtaagccac cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca
    40861 atacggtgtt tttcgctgga ggtcagacgc gcggtgcgca tatcttccgg gatatccagc
    40921 ggcaggtaga gcgatggtaa caccagctgt acggcttcat cccacgggtt atcggtgccg
    40981 tgaccgtacc agatatttgc cgcgctgaag cggcttaccg accagcgcaa catgtcctga
    41041 atggtttgca gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg
    41101 gcatgatcgc aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc
    41161 acgcgcggtg agtgaggaaa aatacgttta aaacgatcga ttgtgctacg agtcgggtaa
    41221 actgtaggaa aattagaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg
    41281 aggaccaggc tctgtttcgc cagttgatgg cggggactcg caagattaag caggacacga
    41341 ttgtccaccg accgcagcgt aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg
    41401 agcaggctga tgccagccat tatttctccg atgagtttca gccgttatta aataccgaag
    41461 gtccggtgaa atatgttcgc ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg
    41521 gcgattattc accggaattg tttttggatt tacacggtct gacgcagctg caggccaagc
    41581 aggaactggg cgcgttgatt gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga
    41641 tgcatgggca cgggaagcat attttgaagc aacaaacacc gctgtggctg gcacaacatc
    41701 cgcatgtgat ggcatttcac caggcaccga aagagtacgg cggtgatgcg gctttgttgg
    41761 tgttgattga agtggaagag tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg
    41821 ccggagcagc gtaaacgcct tatccggcct acaaatatgt gataattcaa taaattgcaa
    41881 ttcacttcgt aggcctgata agacgcatca gagcaatact acgaactaca gttcattcaa
    41941 tacatactga aaacgacacg tcgaatcaat cggttgccca ctactggtat tcacattcac
    42001 cacaatatcg tgtagtactt tatccgttac cggatgacga gttggcgtaa ccgttaccgt
    42061 tgatttatca caaccgtaag aggtaaaggt taacaattct gataccgcgc ggcagtatgc
    42121 tcctctgcca tcattatcag aaaaattact gccatcacag agtctgccgc cttccatcag
    42181 gtaggtcgaa agcgtggtgg tcactgttga ccccgtggac tgaattcgta acgccttatt
    42241 atggctgaca taattttcac tccaggagat atcgtatgaa gtgaccggta tcgttgggca
    42301 tattgtgtta tgcactgtag gggccgtata gcttccaaat ccgggtaaat ctgtatagga
    42361 ataaaaggat gcgatccaag ggcaaccata atcatcagat gcaacggtaa ttttaaacgt
    42421 catatatgca caacgaaaaa ttttcgtatc tatgccaggg caatttgagt tattaattgt
    42481 atcaaagtca gtcagtacca aagttggagc tgaacccgac ccagaccagg aaataacccg
    42541 caacgtatcg cctttatacg caaaagaatc atatttccca tatgtaacct cctttggcgt
    42601 tatcaccgta acattgaaaa cactgctcgc tacagcaccg gttaccttgt ccatggcata
    42661 ataaactctc acactgtcaa aactcgcact cataagttta tctccagcat aactcttaga
    42721 tgcgccgaca acactcagaa cgagcagcat taggctcgcg aaaattatgg tccatttttt
    42781 catttcagaa atactcaaag gttgccgtca ctgtggcact aaagtctccg gacgttacat
    42841 ctcgcccgct ctttgcctgt aaccaggtat taaaatttag gctgttattt ccctgattca
    42901 gcgggagcgt gacgcctgtt gtattgttga ttggcaccga tgtgcctgcc gcagtttcaa
    42961 taccaatccc agcgccggat gctgatgacg aggagtcaaa ggccagaaag ccgggtagag
    43021 cactatcttc tgttcctgtc aacgttactt tgacattaaa caatgtagaa ctatgacaat
    43081 ctttcaattg aaacaccaat ggcactcgct ctgactgccc aaggttcatt aaatcgctgg
    43141 cggcaatggc aggaaaatgt acttctgcca actggctacc attaatcact ggcgtacacg
    43201 atttactgat taaattgccg gtaaagtgca aattatctcc tgctattgtg gttccactgc
    43261 ccagcaacat caggctacta ataaaaaaca acgtacgctt tttcttcatt ggtaatctac
    43321 ctgtaacgtg gcccaggcct caaagtccgc ctctggcaga acgacaccac tcttttttac
    43381 tggcacagcc gttaacaccg gtaaatcggt ttcattcacc ataagtggcg tattgatcgt
    43441 aaaactctgc cctgcctgtt tgagctgaat gcctaaacca gtgatattgc tgcttactgc
    43501 agcattatca aaatcgctta gtgtgccact ccagctcaag gtcatttcca ttaatgggtc
    43561 gcgaaccgcc gtatcgcagg taatggaata cggcacggtt tgctcataat tgctgccatt
    43621 aatgtcatca ataagcacat cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt
    43681 gcagttaggc ggctgcacta aataaccgtg aaaagagacg tttgagagtg ccatcgagca
    43741 aaagccccac agcaaaataa gcgagatccg tttcattggt aatccaccac aattgtggca
    43801 ctggcgttaa attcagctgc cgctaacgtt gtaccactct gtttcaccgg cacagcttcc
    43861 agaacgggaa ctcccgagga aaaattaaat ggcaaccatg agccagaggt taagtcgagt
    43921 attgtgtgat cactactttt ctgaacccga ataccaaacc caggaatacc cgtgctgata
    43981 actgtttcac cgttaatcac gaccgttgtc gcctgcatct gtagacgcag gtcatccatt
    44041 gccagattgt tacaaacaag cgagtagtca atgggacgtt tataatcaac cccattgatc
    44101 ttattaatga atacattacc aaactcaact gccgcaccat ttaccgtaca aggaggaggg
    44161 gcatcgacaa gcacccgtaa attcatactt actgacttac tcgctgcaac tgcaaagccg
    44221 ctaccaacac aacatatcag taacccagtt accgcccaga cagaacaaga tcctttcata
    44281 ctggctctcc cggcctcagc ctttgcggct ctgctcttca tcgacctggc aactgttacc
    44341 gttgcaagtg aaaaagagcg gcatccgagc gccatagtcg ttgacatagg tcagtaccgg
    44401 cacgctaccc atcttcacat tcagcggtgc agaggttttc ggcgacatca ctattggcga
    44461 gaaaccggct gcaggattgc catttttctg tgtgctggca ttactgaaaa tgacgtaata
    44521 cggcgtcggg ttattgacgg taaattcctg accgttgcgg gttaaagtaa ccttatattg
    44581 ccaggggttt ttcatgctga cgttctccag cgccttcggt cgccagaaca acttaatacg
    44641 cgtttgtagt gctatttgca gcgtgttggc tttgtttgat tttggcggga tctcacgcac
    44701 gttgaagtag aacaggcttt cacgatcggc aggcaatttg ttgatatccg gcattccctg
    44761 cacttttacc tgcccgttca tcattgaatc gatgcgttgc accggtggca gaacagttaa
    44821 cggtgaactg atcttgttac ccttttcatc ttcgatccag ctttgggcca gataaggcag
    44881 tttcggatcg ttattgcgca acgtcacgct gattgatttg tcgctttcat taaatatcaa
    44941 acgggtacga tctggcgtta cagaagcctg ggcgacaaac tagcagcgct aagcagtaac
    45001 gctagcgtcg tagcacccaa tttggcagag caaagtaaat cagacatgga tcattcctta
    45061 atcacgttat tggttagctg aaacagaaac agggggattg gtgggtgtca cctgttgcgt
    45121 ctgctcctga atgacaggct ttatgtcatc aggaacaacc ggcgcgacgt tgcctttatg
    45181 ctggcatggc agtaacaagc cgttaaacag atcggcaggc agcgggtccg gcaggttgat
    45241 atcgcaatgc gcaacaccac tccagaacac actcatgtgt tcgccaggtt tcaccccagc
    45301 cagataaaca ttgccgtcat catcgacaag gcccactgtc tgctcgttat cattttttac
    45361 ttctgcgcca aacggaggat ggctgccgtc ttgtaaacgc agcaccgcca tcgctttttg
    45421 accactgatg acggcaaatt tgcggtagcc aatcgcccct tcagttagcg tggcttgtac
    45481 caccgactgg gttgcttcgg cgttttccgg cagtttgttg aggtcgatat acgcctgatt
    45541 gcggtaatag ttattgacgt cagaaacgac ggctttacca aacatattgg tataaacagc
    45601 agcaccgtta ccttctaccg gaacatcggc aacgccatcg gcatcaatca acaagcgtgt
    45661 cccgcccata ttctgggtac ggtgaagtgc gccaccgtgg gcagtaagcg ttgcgccgcc
    45721 ctgtaacgac aagcccgcag aggtgtattg cccttcatgg tagttcgcac tgaggtcaac
    45781 ctgcgccagc gaaccatcat ggctgtaata gccgtcaacg ctggtgtgtt tgtcactggt
    45841 gccgacgttc aactgatagt gagtcgcgtc atcgacacgg ctgaaataac cgacctgact
    45901 gctgtccgtc ccactgccat agttaccgtt atagctaacg gtactgttgt caccccacgg
    45961 catactgagc gaaatgtaca tgcctttgtc ggcctggttg tcatactcgt aacggtagcc
    46021 agtcatcgag atgctgacat tacgaatact gcccatattg aaatagtgcg agagcatgat
    46081 gttgtagttt gtctgctcct cgcgatccca gtaggtatgg cgggtatagt tgagataaac
    46141 cgaaacaccc gcatcgcgga agttctggtt ataagtggca gtgtacatct ctttgtcgtt
    46201 gcccgtgcgt accattccgc tgtcactggc atccagatac tcgctcatgg tcataaagtt
    46261 ctcttccgag aagcgatatc cagcaaaagt aacgcggctg ttgagctggt caaagtcttt
    46321 ggaataactc acacggaagg agttaccgtc cagcgaacct ttgccataag cggtgtcttt
    46381 atccagtttg gtgtgcgagt gagtaacatc aaacgcgacc gcgccgaatg tagacaaatc
    46441 gcgaccgacg ccaagcgccg cagactgata gttttcatct cccagtgcgc cgccatataa
    46501 cgaccagccg ttagcgatcc cccaggaagc ttcagcacca gaaaaaaatt caccctcgac
    46561 atggtgtccc cactcttgcg gacggcccat catgatctta tagcgaacct gacctggacg
    46621 agtgaggtat ggcatcgagg cggtgctgat gtcatattcc tgcacctggc cgttctgttc
    46681 ttcaatgcga atatgcaacg taccggagac agaatcacca agatcctgaa tacgaaacgg
    46741 tccagccgga acctgcgttt cgtaaatcac acgccccatc tggctgacgg tcacttttgc
    46801 tgtggtgtgt gccacgccgg aaatgtctgg cgcgtagccg cgcagattgg gaggcaacat
    46861 ttgatcgtca gtactgacac tgccaccaac atagttaaaa ccatcaaaaa tatcggatct
    46921 gaggtaatcc tcgcccagcg ccagtttggc ttttaatgat ggtaacgccc gctaggcata
    46981 gtagcgactc cactcccatt ttttttgagt ttcatcgccg ctgaattcat cgtcatcatt
    47041 actgcgagta tgttgatagt tagtctgcca gtcagcacgc atacgccacg gccccaggtt
    47101 aaccccgacc gtcccgttgc cgctgatctc gttactatca tcaccgccat tttcttcgtg
    47161 ccgggtttgt gcgttgatgc tgtagtccgc gacgatcccg gagatgccgt catcccaacg
    47221 tgaaggcgga tcccagtcgg gataagtata ttcgaggtaa gcctgtggca gtgaaatgac
    47281 taatgcggac tggcttaaat cagccttaat ttccatgcct tccagttgac cggatttcag
    47341 gcatttagca tcgtggctcc attgcagatt tttcgccacg tcttctttta aaccaaactg
    47401 cgctaccagt tccggtgtca gacaagcata gcttttgctc gcgtcatctt caccagcata
    47461 ccagtaaata tcgtactctt ccgccaatgg ctgtttattt agttgaacct gtaaattgta
    47521 ttttccgggc tcaacatatc cctggctgga aaaacgcttc agatcaattt ttgtgtcgcc
    47581 ttttaattcc agaaaacggg aatcaaactg aatgtcgtct tcagcccaga cactgctata
    47641 actccctgac attgccagcg caatgcacca cggaagaatc cgtaatcgaa aaagagaatg
    47701 gtcaggcata cacgtttcta tccgttttta ttcttgttcg gtcaaaatcc atttaaatcc
    47761 cccatagcag tgtcgctatg ggggtatgcc ttacaggtaa gtaatctgga aggtggtatt
    47821 tgcttcgaaa ttacccagat ctggcgcatc agctgcgccg accagccagg ctttaaagtt
    47881 cagtgtctgc ttcgctttgc ctttattggt agaagtgtct ttcactattt tctggtcaat
    47941 acccatgccg cttttgtaag aggtacccag agagtcaccg atcgccaggc taacattgtt
    48001 aaatgccgca caggtatcgg tattgaaaat ggtgtaataa ttgccgctat ttgcagaaga
    48061 aacggtacca gtaaaggtag tggtcattgt ttcctgagta tcaaatacgc aatcttgcag
    48121 acgaatctgg aaacttttcg gcgtggtggt gccgttattt ttcagttttt cggcaccgat
    48181 atcacccata tcaacttcca gtttgtgatc gtccggaacg atagaacaca ctgagtcttc
    48241 aatggtgccg taaaaacgtg cagtaccgtt gttacccgca gcgattgttg ccgtagaggt
    48301 gaacacgccc atcaccattg ccgcagcaat agctgtttta acaaacttac tcattatttg
    48361 tccttaaact ataaataatt aaaaatataa acattgcaat acattgattc agtcaataac
    48421 caatgtttat acccagccat tgaatacggc atgcgatcca aaaagaacag gtcgtagaaa
    48481 attgtattcg ttggtgtggg gccgcatagt gatgtttcca cacccct
//
LOCUS       DAZRUF010000004        45617 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZRUF010000004 DAZRUF010000000
VERSION     DAZRUF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.004"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(32..1891)
                     /gene="gss"
                     /locus_tag="ACHXOL_000170"
     CDS             complement(32..1891)
                     /gene="gss"
                     /locus_tag="ACHXOL_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR6807259.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2096..2962
                     /gene="yghU"
                     /locus_tag="ACHXOL_000171"
     CDS             2096..2962
                     /gene="yghU"
                     /locus_tag="ACHXOL_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR6807260.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(2991..3072)
                     /locus_tag="ACHXOL_000172"
                     /pseudo
     CDS             complement(2991..3072)
                     /locus_tag="ACHXOL_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3085..3333)
                     /gene="hybG"
                     /locus_tag="ACHXOL_000173"
     CDS             complement(3085..3333)
                     /gene="hybG"
                     /locus_tag="ACHXOL_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR6807261.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3346..3687)
                     /gene="hypA"
                     /locus_tag="ACHXOL_000174"
     CDS             complement(3346..3687)
                     /gene="hypA"
                     /locus_tag="ACHXOL_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR6807262.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3680..4168)
                     /gene="hybE"
                     /locus_tag="ACHXOL_000175"
     CDS             complement(3680..4168)
                     /gene="hybE"
                     /locus_tag="ACHXOL_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR6807263.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4161..4655)
                     /locus_tag="ACHXOL_000176"
     CDS             complement(4161..4655)
                     /locus_tag="ACHXOL_000176"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR6807264.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4655..6358)
                     /gene="hybC"
                     /locus_tag="ACHXOL_000177"
     CDS             complement(4655..6358)
                     /gene="hybC"
                     /locus_tag="ACHXOL_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR6807265.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYGGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6355..7533)
                     /gene="hybB"
                     /locus_tag="ACHXOL_000178"
     CDS             complement(6355..7533)
                     /gene="hybB"
                     /locus_tag="ACHXOL_000178"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR6807266.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7523..8509)
                     /gene="hybA"
                     /locus_tag="ACHXOL_000179"
     CDS             complement(7523..8509)
                     /gene="hybA"
                     /locus_tag="ACHXOL_000179"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR6807267.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8512..9630)
                     /gene="hybO"
                     /locus_tag="ACHXOL_000180"
     CDS             complement(8512..9630)
                     /gene="hybO"
                     /locus_tag="ACHXOL_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR6807268.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9819..10106)
                     /gene="yghW"
                     /locus_tag="ACHXOL_000181"
     CDS             complement(9819..10106)
                     /gene="yghW"
                     /locus_tag="ACHXOL_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR6807269.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10225..11112)
                     /gene="yghX"
                     /locus_tag="ACHXOL_000182"
     CDS             complement(10225..11112)
                     /gene="yghX"
                     /locus_tag="ACHXOL_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR6807270.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11269..12309
                     /gene="gpr"
                     /locus_tag="ACHXOL_000183"
     CDS             11269..12309
                     /gene="gpr"
                     /locus_tag="ACHXOL_000183"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR6807271.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12349..12843)
                     /locus_tag="ACHXOL_000184"
     CDS             complement(12349..12843)
                     /locus_tag="ACHXOL_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR6807272.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13034..13918
                     /gene="yghA"
                     /locus_tag="ACHXOL_000185"
     CDS             13034..13918
                     /gene="yghA"
                     /locus_tag="ACHXOL_000185"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR6807273.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14078..14503)
                     /gene="exbD"
                     /locus_tag="ACHXOL_000186"
     CDS             complement(14078..14503)
                     /gene="exbD"
                     /locus_tag="ACHXOL_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR6807274.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14510..15244)
                     /gene="exbB"
                     /locus_tag="ACHXOL_000187"
     CDS             complement(14510..15244)
                     /gene="exbB"
                     /locus_tag="ACHXOL_000187"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR6807275.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15496..16683
                     /gene="metC"
                     /locus_tag="ACHXOL_000188"
     CDS             15496..16683
                     /gene="metC"
                     /locus_tag="ACHXOL_000188"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR6807276.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16823..17482
                     /gene="yghB"
                     /locus_tag="ACHXOL_000189"
     CDS             16823..17482
                     /gene="yghB"
                     /locus_tag="ACHXOL_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR6807277.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17522..18421)
                     /gene="yqhC"
                     /locus_tag="ACHXOL_000190"
     CDS             complement(17522..18421)
                     /gene="yqhC"
                     /locus_tag="ACHXOL_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR6807278.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESALQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18615..19778
                     /gene="yqhD"
                     /locus_tag="ACHXOL_000191"
     CDS             18615..19778
                     /gene="yqhD"
                     /locus_tag="ACHXOL_000191"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR6807279.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19883..20710
                     /gene="dkgA"
                     /locus_tag="ACHXOL_000192"
     CDS             19883..20710
                     /gene="dkgA"
                     /locus_tag="ACHXOL_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR6807280.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20910..21836
                     /locus_tag="ACHXOL_000193"
     CDS             20910..21836
                     /locus_tag="ACHXOL_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR6807281.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21887..22144
                     /gene="yqhH"
                     /locus_tag="ACHXOL_000194"
     CDS             21887..22144
                     /gene="yqhH"
                     /locus_tag="ACHXOL_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR6807282.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22187..24406)
                     /locus_tag="ACHXOL_000195"
     CDS             complement(22187..24406)
                     /locus_tag="ACHXOL_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR6807283.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24517..25929)
                     /gene="ftsP"
                     /locus_tag="ACHXOL_000196"
     CDS             complement(24517..25929)
                     /gene="ftsP"
                     /locus_tag="ACHXOL_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR6807284.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26004..26741)
                     /gene="plsC"
                     /locus_tag="ACHXOL_000197"
                     /gene_synonym="parF"
     CDS             complement(26004..26741)
                     /gene="plsC"
                     /locus_tag="ACHXOL_000197"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR6807285.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26974..29232)
                     /gene="parC"
                     /locus_tag="ACHXOL_000198"
     CDS             complement(26974..29232)
                     /gene="parC"
                     /locus_tag="ACHXOL_000198"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR6807286.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(29778..30260)
                     /locus_tag="ACHXOL_000199"
     CDS             complement(29778..30260)
                     /locus_tag="ACHXOL_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR6807287.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30313..30705)
                     /gene="ygiW"
                     /locus_tag="ACHXOL_000200"
     CDS             complement(30313..30705)
                     /gene="ygiW"
                     /locus_tag="ACHXOL_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR6807288.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30857..31516
                     /gene="qseB"
                     /locus_tag="ACHXOL_000201"
     CDS             30857..31516
                     /gene="qseB"
                     /locus_tag="ACHXOL_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR6807289.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31513..32862
                     /gene="qseC"
                     /locus_tag="ACHXOL_000202"
     CDS             31513..32862
                     /gene="qseC"
                     /locus_tag="ACHXOL_000202"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR6807290.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            32972..33553
                     /gene="mdaB"
                     /locus_tag="ACHXOL_000203"
     CDS             32972..33553
                     /gene="mdaB"
                     /locus_tag="ACHXOL_000203"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR6807291.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            33584..33898
                     /locus_tag="ACHXOL_000204"
     CDS             33584..33898
                     /locus_tag="ACHXOL_000204"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR6807292.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(33946..35838)
                     /gene="parE"
                     /locus_tag="ACHXOL_000205"
     CDS             complement(33946..35838)
                     /gene="parE"
                     /locus_tag="ACHXOL_000205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR6807293.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(35867..36448)
                     /gene="yqiA"
                     /locus_tag="ACHXOL_000206"
     CDS             complement(35867..36448)
                     /gene="yqiA"
                     /locus_tag="ACHXOL_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR6807294.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(36448..37275)
                     /gene="cpdA"
                     /locus_tag="ACHXOL_000207"
     CDS             complement(36448..37275)
                     /gene="cpdA"
                     /locus_tag="ACHXOL_000207"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR6807295.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(37300..37722)
                     /locus_tag="ACHXOL_000208"
     CDS             complement(37300..37722)
                     /locus_tag="ACHXOL_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR6807296.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(37723..38352)
                     /gene="nudF"
                     /locus_tag="ACHXOL_000209"
     CDS             complement(37723..38352)
                     /gene="nudF"
                     /locus_tag="ACHXOL_000209"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR6807297.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            38557..40038
                     /gene="tolC"
                     /locus_tag="ACHXOL_000210"
     CDS             38557..40038
                     /gene="tolC"
                     /locus_tag="ACHXOL_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR6807298.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40186..40857
                     /locus_tag="ACHXOL_000211"
     CDS             40186..40857
                     /locus_tag="ACHXOL_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR6807299.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            40863..42023
                     /locus_tag="ACHXOL_000212"
     CDS             40863..42023
                     /locus_tag="ACHXOL_000212"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR6807300.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(42061..42849)
                     /gene="ygiD"
                     /locus_tag="ACHXOL_000213"
     CDS             complement(42061..42849)
                     /gene="ygiD"
                     /locus_tag="ACHXOL_000213"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR6807301.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            42992..43765
                     /gene="zupT"
                     /locus_tag="ACHXOL_000214"
     CDS             42992..43765
                     /gene="zupT"
                     /locus_tag="ACHXOL_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR6807302.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(43823..43993)
                     /gene="yqiD"
                     /locus_tag="ACHXOL_000215"
     CDS             complement(43823..43993)
                     /gene="yqiD"
                     /locus_tag="ACHXOL_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR6807303.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(44254..44907)
                     /gene="ribB"
                     /locus_tag="ACHXOL_000216"
     CDS             complement(44254..44907)
                     /gene="ribB"
                     /locus_tag="ACHXOL_000216"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR6807304.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45011..45159)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            45281..45571
                     /gene="ubiK"
                     /locus_tag="ACHXOL_000217"
     CDS             45281..45571
                     /gene="ubiK"
                     /locus_tag="ACHXOL_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR6807305.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11256 a        12243 c        11460 g        10658 t
ORIGIN      
        1 cataatcagt acggggttgc tacaacatgt attacttttt caccacaatt aacggttcaa
       61 tatcactctc ttttttgatg accagtgatt catcaccgcg caaacacgtc ccaccgtagt
      121 tgccgcccac ggtgaaggta catacctgaa tgtatttacc gtccactttc ggcaaacacc
      181 acagttgctg atagatgttt ttctgctcgg caaatttacc gctggttttg tccagcacct
      241 cttcatgatg gctgaccagg tcgatattgc taccacagcg accggcaatc ggtttcactg
      301 cgtaacctgt tttcaccaat tcatcattaa cagtgaaatc ggtatccagc aggtaacgat
      361 ggtgcgggaa cagcgaccag aggatcggca gaatcgcttt gttgccgggg atcaccgtcc
      421 acagcggctc aaagactagc acttccgggc gcagcaatac gtcgataaga cgcacttcgt
      481 tttgcggatg accggtacgg attggcaccg cagcaaactc acggtcgcta acttcacgaa
      541 tctgatcaaa cgcggtttcc cacgcccagg ttttccacac gcagttaacc agtcgccctt
      601 ccccatcaat cagttgcccg gcagcatccc agcccagttc atccaatcca cgcaagatac
      661 gcgtttcaaa gcccgcctgg tgcagcgcct gctccataaa ctgcgcgtga tagttttcct
      721 cgatatcttt gtcctgcatg atatggacaa acggacgtgc acgactgtgt ttccaggcac
      781 cagccaattc gttaatcagc ccttccgccg gattgaagcc gttgccttta tagccctgct
      841 ccgcccaacg ttcgaggatc aagcccgctt cggtatgaca ggaggcggag tcggcgttgt
      901 actcgtaaac cttcaggcca cgctcatcca tgcagaaatc catacgacca gtgatcatat
      961 ggtgacggcg acgctgccag gagagacgca aacgtggcca gaggattttc gggatgtcga
     1021 acagcgccag caggttgtca tctttcagca ccttgtcggt tgcgtgaaga tacatcaggt
     1081 gcagctcgtt ggtggcttta attagctcct gctcggcact ctcggtaatg gtgtagtaat
     1141 gataaggatc ctgattgatc acctgaccgt tggcctgcac ataggcgttt tgcagcggat
     1201 ctttttcatc cagccattta ccgtcaaact ggcctttgtt ttccaggcgc gctccgctga
     1261 ttttcagcag ctcgcctgca atttccggct gcggtaagct gtattcagta tcttccgtct
     1321 ggatcatcca gcccagaatg gtggtgtcat caaaagtgtc tttcagggta tagcagccgt
     1381 tttcgaccac catctccagc tcacgcgtcc actgttgccc ttgcggcaac ggggaatgaa
     1441 tcacgttctg ttccgcaata cggactttgt tgccatgcaa ttgggtaatg atggcgacat
     1501 ggccagtgtc tttaaattca ccgcctttat cccagataag aagcgcaccc gcgaccggcg
     1561 cacgcggcga gccgttagga aatgcctgca atggcaggat gttgtcatta accacttctc
     1621 gcaggaagcg cagcgagaaa atctcccacg ccatacccac gtcagtaaag accacaccgt
     1681 aattcagaaa gagaaaacgg cgagcaaatt caacgcattg ccacttgtgg cccatatatt
     1741 cgtcgtcgat atagctacgg aatacggcgt catcttcgta ttcctgcgga tcgagagaac
     1801 tgtaatctga agagtagatt gctaccccac ctggggcgta gcccaataat gtcccgaacg
     1861 gggcatcctg gctggtcgtt cctttgctca tcactttacc tttaacaata caacctaagc
     1921 agttggcgta attgttgtgc tctgattacc tgctcagcga cattaaccgg acagaggtca
     1981 gactaatcat acacctcatc gcagcggctg gcgcaaaggg ttaaaaaatt cacattctgt
     2041 acagcaagtg acctgctaca ctgcttcaac actaccactc agaaggcaac tcactatgac
     2101 agacaatact tatcagcccg cgaaagtctg gacgtgggat aaatccgctg gcggcgcgtt
     2161 cgccaatatc aatcgcccgg tttctggtcc gacgcatgaa aaaacgctgc ccgttggcaa
     2221 acacccattg caactttatt cgctgggaac gccgaacggt cagaaagtaa cgattatgct
     2281 tgaggagctg ctggcgctgg gcgttactgg tgcagagtac gacgcctggc tgattcgtat
     2341 tggcgatggc gatcaattct ccagcggctt tgtcgaagtg aacccaaact cgaagatccc
     2401 ggcgctgcgc gatcatacgc ataatccgcc gatccgcgtg tttgaatctg gttcgatcct
     2461 gctttatctg gcggagaaat ttggctactt cctgccgcag gatttggcaa agcgtactga
     2521 aacgatgaac tggctgttct ggttacaggg cgcggcaccg ttcctcggcg gtggttttgg
     2581 tcacttttac cattacgcgc cgatgaaaat tgagtacgcc atcaaccgct ttaccatgga
     2641 agccaaacgt ctgctcgacg tgctggataa gcaactggcg cagcataagt ttgttgcggg
     2701 cgatgagtac accattgcgg atatggcgat ttggccgtgg tttggcaatg tggtgttagg
     2761 cggcgtgtat gatgccgcag agtttcttga tgcgggcagt tataagcatg tacaacgctg
     2821 ggcgaaagaa gtaggcgaac gtccggcggt gaaacgtggg cgtattgtta accgcaccaa
     2881 cggaccgctg aatgagcagt tgcatgagcg ccatgacgcc agtgatttcg agacgaatac
     2941 ggaagataag cgtcaggggt aagggttggt gttcgtcgca gcaagccatc caggccggat
     3001 aaggcgttca cgccgcatcc ggcaatcgtg cacaatgcct gatgcgacgc tgccgcgtct
     3061 tatcaggcct acgaaagcta atcatcacgc gctggtaatg tcgtaatcca tttggcgcag
     3121 tgcgtctaat gtggctttgg cttcatcttc gtcgatgatg ctcatggcaa atccgacgtg
     3181 taccagcacc cactggccca gtagatcggc agggttacct tcacaaatca gggcgatatt
     3241 cacgtcgcgc ttgataccac atacttcaac ctgcgcaagc tggtgaatat cttcaccgac
     3301 agccagcacc tggcctggaa cgccaataca cattattaac tccggttatt caacttcaat
     3361 acttttgacg atcagcgaat cgccggtatc gacacgcaac cgctcgccgt gacagagcgg
     3421 acactgcgca tcgtgctgat gaatctccac cacctggctg caatcccagc accaagcctg
     3481 ggcgggttta tagacgatat gtaaatcgca cccttgcgcc accgttccgt ggcagacaat
     3541 ttcaaaacta aaacggacgg cgctctcctc aacgcaggag agcgcgccaa tttccagcca
     3601 cacggcggtg acgcgcttaa catcgtgctg ctccgcctgc cgttggataa tttcaacggc
     3661 gctctggcaa agagacaact catgcatttt cgccactcct gcgaccaaac agcagggcgc
     3721 gacgccctgc gtgtggtaca tccggattcg tgactggcag cgaaaggatc attcgtgcgc
     3781 agtcatccgt caggcgttgg ccctcttcaa tcgacatgct gtgcgaaagc ggcgacatca
     3841 gcgaacagga gagatattgc gaaacaccgt ccagttcgcc aacggtaaag gtcatagtgc
     3901 catacggcag ttgcagacca attttttcac tcactttgcg cagcggccag agttgatccg
     3961 ggccggggaa gataactgca ctcagcatcc acggggtgat cacacacccc gtccactgac
     4021 cttcgaacag cgtaaaatca gaaacataca ccggcattga aggatgcaga aaagagagat
     4081 cgtgcatcga acgccgggca atttcttcaa acgctgcctg tacttgcgcc ttcggggagg
     4141 tctggaaacc tgctatctct tcagtcatga atcgcctccc gtgggatggc ttccacgcct
     4201 gattcgcgca gcgcagccag aacctgctca agcgcaggtt caatcattgc ttcaaccgtc
     4261 ggcgttaagc cgatgtgtgg ctccagcgat tccgggatca cgccgaccag ggtcagcttt
     4321 ttcggaaact caccggtgaa gcgcagggcc gacaatacgt cggccaggcc aagctgatgc
     4381 ggagagattt tgttggtaaa caacgccgga acttcttcat cccgcaggat catcatcgtt
     4441 cccggcgtgt tctttttcga cacaatggca tccgcaataa tcagatgatc gcgatttgcc
     4501 atgtcgccaa gcagctccat tcccgccgtg ccgccatcga ggatctcaac ataatccggc
     4561 agaatgtatc gttgctctaa cgcttcgaca atccgcacac cgatggcttc atcggtcagc
     4621 aaaatattgc cgacccctaa gactaaaata cgcattacag aaccttcact gaaaccactt
     4681 cgttgccgtc ggcatccact acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa
     4741 tggtacgcac cacttccagc ggtttattcg gatcagcaac cggtgtaccc accagcgact
     4801 gctcgtaagg accgacgtca tcattgaagt tacgcggacc agagttccag gttgatggaa
     4861 caactgcctg gtagttactg ataataccgt ctttaataac catccagtga gagagcatac
     4921 cgcgcggcgc ttcgaggaag ccaacacctt tgaactcacc cgttgccgga atgttcggtt
     4981 tcacaaaggt ggtgtgatcg cctttgccga tattggtgat cagtgcactg tattgatttt
     5041 gcaggatatc ctgcaattcg cagcagtgaa cggtacgacc aataatacgg cccagcgtgg
     5101 agtgcagctg cgccacttcc agcgtgttgc cagtcagttt ctgataaatc gcaacgattt
     5161 cattcagttt gttctgggta gattcgcgac ctgccgccag tttcaccagc atattagcca
     5221 gcggacccac ttctaccgtt ttgccgtaga aagtcggtga tttcacccag gaatatttcc
     5281 cgtcgtcaga ccaaccatca taagccggaa tggtggtacc ttcccacggt gcctgcggcg
     5341 cttcgtcttt ataccaggag tgcttcgcgc tttcctgaat gcctttgatc aggtattcat
     5401 cggaatgaga agtgatcgga cgatacgagg acagatccgc attctcaatg tagccgcccg
     5461 ggaacaggaa gctgccgttt ttactgtcgg tcgggaattc cggcacgctc aggtagttca
     5521 ccgcaccttt accgcgcgtc agccattccg ggtagaacgc ggcaataact gcggtatcaa
     5581 ccttataaac ctgttcaaca aagtcgctca gcttgtcgat gaaagacttg atgtacatca
     5641 ggcgctcaag gttcagcacg cccaaaccgt cgaggttgat cgggttcgcg acaccaccta
     5701 ccgccaggtt ctgaatgtgc ggcgttttac cgcccagcag cgccacgacg cggttagcgt
     5761 cacgctggca ctccagcgcc tgcaggtagt gcgctaccgc aatcaggttc acttccggcg
     5821 gcagtttcat cgccgggtga cccccgtagc cattggcgaa aatacccaac tgaccgctgg
     5881 caaccagatc tttgatcttg ttctgaactt tagtgaactc ttccggactg ttcagatgcc
     5941 aggtcgaaac gcctttcagc atttcggagg ctttggttgg gtcagcttgc agtgcagaag
     6001 tgatgtccac ccagtccagc gccgaaagct gatagaaatg aacgatatgg tcatgcgtgg
     6061 tgtgcgcagc cagaatgatg ttacggatgt attgcgcgtt aaccggaacg tcgatattca
     6121 gcgcactttc tgccgcacga acggaagaca gcgcgtgagt ggtagtacat acgccacaga
     6181 tacgttgcac aatcatccat gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc
     6241 cgcgccacat ggtaccggaa gcccatgctt tcgaaacgac gccattttcg atttcgcaat
     6301 cgatgcgtaa atgcccctca atacgggtta ccggatcaat agtaattctc tggctcatgc
     6361 tttgctcgcc tcatgacgat tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag
     6421 acgaatgagt acgatgtaag cgcaaatctc aatagccaca aaaccaatag aaatcaacag
     6481 ttcttcccag gtcgggaagt aggcgtaacc gccgcccggg ttgaatgcca ccagcgaata
     6541 ggtcagacgc caggttgcac aacctaacag tgcgctcagt gctgacagga acagcatgcg
     6601 ggaatcatta cgcagcttcg ccacacgcag aacgaccagc gggaagagca tcagcaggac
     6661 ttcaatccag aacatcacgg agtagaagtc accggcaaac gctaacgaca gcttgtcgcg
     6721 atagataagc tcgccaaagc gcagcacgat gaaaatcgcc agcaacacac tgatggtgtt
     6781 ggtcagcttg acgaacagac tcttttcatc cggaccgttg ccacgcagac ccgcctgcac
     6841 cagcgaacct tcaaagatga caatcgagaa gcccatgatg aacgccgtca gcagcgagaa
     6901 cagcggcaac atttcatagc tctgccacaa cggatgcacc ttgtagcccg ccgagatcat
     6961 cagtgacccc attgaagact ggtgcatggt cggcaacagc gcgccaagtg caatgatgaa
     7021 gaacataacc ttgttcagac gcttgagaga aaccttccag cccagacgtt caaacagtgc
     7081 cggagcaaac tccagtgcca tcacgccgat atagatggtc atacagaccg ccgtctcgaa
     7141 cagtaccgag ttcacgttga agtgacccgg aatgtagaag tacggcaggt tccagtagcg
     7201 acccacgtcg atagtgatcg acaagccacc cagtgagtaa ccaaacagac tcgccaacag
     7261 cgccggacgc accagcggat ggtattgccc acggttaaag acgtataccg cccacgccag
     7321 cgcccagccg ccacaggcaa agccggtgcc aatcagcagg tcaaacgcga tccacacgcc
     7381 ccacgggaag ccgccgttca ggtcagagac agagcccaga ccgaacacca gacgcttcac
     7441 aatcaggagc atacagatga cgattaacgg tccaaaaatc atgaccggtt tactgatgat
     7501 tttgccgccc agcggttgtg gatcatgact catgatcgtc tcctccgtcg tgatggtcgt
     7561 ttttggtgtt gcgacgaacc agcacggtta agcccgccag cacagccagt ggtagcatca
     7621 tgcctttata cagggtgtgt tgaatatttt cggaacgcgc accggtagaa agatcgtcca
     7681 gtttcggcag gtcgagattt tcataaggca cacccgtcag taccagaacc tgagtaccgc
     7741 cgccctcttt ctcgccgtac agatgcggat aatatttcgg caccgtatgc aggtaagtgt
     7801 cgccagattt cagcgtctga cgcggatagt ggtattcgct gccaggcttc agcgccagac
     7861 gttttttcgc ctccgccatc agctcttcac gcgtaccgaa aatcaccgcg cccgccgggc
     7921 acacttctac gcagccaggc agaccgcctt tatcgagacg ttccacacct ttctggttgc
     7981 acagctcgca cttatgcagc gcaccaaacg ggttgttgta gtcgtacttc ggcacgttgt
     8041 acggacaggc gaccatgcag taacggcaac cggtgcacac gtctttgtcg taatggacaa
     8101 tgccggtttt cggatctttt ttcagtgcag agaccgggca cacagagaca cagttcggat
     8161 cgacgcagtg catacactgt ttcttaatgt acgcgtagcc gttctcctcc tggtctttgt
     8221 tgacccctgt gccgctggtc cacacctgaa tgatgttatt ggtatacggc gacagtttgt
     8281 cgttgttcga ccaggtctgt tccccttgcg ggttacgttc agggaagttg atatcctgac
     8341 acttggtgac gcaagcctgg cagcctacgc acaaggtcga gtcgtacaac atccccagcg
     8401 atcccggaat tggcgggcgg ttttcagcag ccgcatgact gacagacggc agcgcgcccg
     8461 tcagcaatgc cccgcaggag gctgctttaa taaaattacg tctgttcacg gttattctcc
     8521 ccgtgagtca gcgttatctt tcttttgctg acgacccagt tcacgcaccg ccatcacgct
     8581 gacaccggca accagcccaa ccacaccgcc gagcaaacca atagcgcctg cagagacgtt
     8641 gccgccctct ttagcgttaa catccggttt ctgtgaacgc ggagtctgat tttcgacgtt
     8701 ggcaagctga tggatgcctt tatggaagcc gataccttct tcgttacagc catagcatgg
     8761 gtgaccaatc gctaccggcc acacgccgcc aacatcgcag aattgcagcg ttgagcagtt
     8821 gccgtaagtt tctggccctt tacagccgag gtggtacagg caccagcctt cacggtggcc
     8881 ttcatcaccg aactctttgg caaaacgacc agcatcgaag tgcgggcgac gttcgcagtg
     8941 ttcgtgaatc agacggccat aggcgaaggt cggacggttt ttgtcatcca gtttcggcgg
     9001 tttgccgtaa gtgatgatgt gcgcaacggt cgcgaggaag ttgtgcgggt tcggcgggca
     9061 gcccggaata ttgataacgg ttttgcctgg cagaacttct tgcaggctga ctgcgccagt
     9121 tgggttaact ccagctgcgg caacaccgcc ccacgcagag caggaaccga tagcgataat
     9181 cgctgctgcg ccttctgccg ctttgcggat gtgatccaca atcggctcac cagcaaccat
     9241 gcaataaata ccgttatctt ttaatgggat ggaaccatcc acaaccaaca catactgccc
     9301 tttgtacttc tcaagtgcgt tatgtttgtt ctcttcgacc tgatgaccga aggcggcgga
     9361 aagcacttcg tgatactcca gagagatagt ctccagcacg aggttttcta ccgttggatg
     9421 cgttgcacga agcagagatt ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac
     9481 tggcggacgc tgcgggttag taaccgattc ggccatctct gcagcggctt tgctacttaa
     9541 ccccatggtg gcggctaatg ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg
     9601 agaatggatg agggtgttat ctccagtcat ttatagttat tccgttgcga agacctggca
     9661 tatattttgc ctcaatcgca aaatcaataa tgcgatcgat gcgccattta ccacacattt
     9721 attatggtta tcgatatcat gatactgcgg cggggcgcta aaacgaaggg aagaagcatt
     9781 agtgtaatta attaaagcaa gataatacgt atgtttgatc aattttcgag gcgataaccg
     9841 gccataaaaa agcgcaacgt agaacaggaa ttattctcac gaaagaaatc cattaccatc
     9901 tctttatcca gtccatagcg gcgcgtcaga atacccgctt cccaggcgct aagctggcga
     9961 tctccggtct tttcgtacat ccggtgtgag tagcctaata caaaaccgcg tttataatcg
    10021 gcacagaatt ttgtcacatt aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt
    10081 aagcctttac caaaatggtt attcatcgtt cacctcattc gctatatatc gccatatata
    10141 gcgatatttt aggcaaagaa cgacttttgc gcacgctatt ttttcatgct tgtttaagac
    10201 gtaatccagt aagagataaa cctatcagga gagatattta tcgaaccatt tcagtgtcct
    10261 ttgccaggca agatcggcgg cagatttgtc ataacggggc gtggaatcat tatggaatcc
    10321 gtgattaacc cccggataga tatacgcctc ataaacctta ttattggctt tcaacgccgc
    10381 ctcgtaagca ggccagccct cgttgattcg ggtgtccagt tccgcgaagt ggagtagtaa
    10441 aggcgcttca atcttcgcca catcggcagt gggtgcctga cgaccataaa acggcaccgc
    10501 gcaggccagt tccggatacg cgacagccgc cgcgttcgat acgccaccgc catagcaaaa
    10561 tccggtaata cccactttgc ctgccgcttg tggatagcgt tgcataaact caattgcggc
    10621 aaagaaatca ttcatcagtt tggttggatc aacctgctgt tgcagctcac gacctttatc
    10681 atcatttccc ggataacctc caacggaact taagccgtca ggtgccaggg cgatataccc
    10741 cgccttcgcc actcgccgtg ccacatcttc gatatacgga ttcagtccac gattctcatg
    10801 caccaccacc acggctggcg ttttgccgct catttttgcg ggtttcacca gataaccccg
    10861 tacctcgccg tgaccatttg gcgaaggata catgatgtac tcagcaacaa tctccgggtc
    10921 ggtaaactct acctgagtcg ccagcgcata atttggcttg agcaaatcaa acaacgctaa
    10981 tgccgtcatc ccgcccaccg catacttcgc cgcaaggttg aggaactcac gtttcgaaat
    11041 tttcccgtga gcgtaatagt cgtagtaatc caacaactct tgtgggaaat ctttggcggt
    11101 taaacgcggc atcgttgcac tcctcagttg gtgttttttt aagcaaagca taagcacgtg
    11161 tttttgccca gtttttcgtc actctgtgag ccagactacg ggatacgcgc tggcgaatcg
    11221 ctaaactaga aacattgttt cgaaattgaa cggtggaaag gagaggtcat ggtctggtta
    11281 gcgaatcccg aacgttacgg gcagatgcaa taccgctatt gcggaaaaag cggtttacgc
    11341 ctgcccgcgt tatcgctcgg tttatggcac aatttcggtc acgttaacgc gctggaatca
    11401 cagcgtgcga tcctgcgtaa agcgtttgat ttgggcatta cgcactttga tttagccaac
    11461 aattacgggc cgcctccagg aagcgcagaa gagaactttg gtcgcctgct gcgggaggat
    11521 tttgccgctt atcgcgatga actgattatc tctaccaagg ctggctacga tatgtggccc
    11581 ggcccttacg gctctggcgg ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg
    11641 aagcgtatgg ggcttgagta tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg
    11701 ccgatggaag aaaccgcctc tgcgctggct catgcggtac aaagcggtaa ggcgctgtat
    11761 gtcgggatct cctcttactc gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag
    11821 tggaaaattc cgcttttaat tcatcaacct tcgtacaatt tactgaaccg ctgggtggat
    11881 aaaagcggcc tgctggatac cctgcaaaat aacggcgtgg gctgtattgc ctttactcct
    11941 ctggctcagg gattgctgac cggaaaatat ctcaacggca ttccgcaaga ttcacggatg
    12001 catcgtgaag ggaataaagt tcgtggtctg acgccgaaaa tgcttaccga agccaacctc
    12061 aacagcctgc gcttattgaa tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg
    12121 gcgttaagct ggttgctgaa agatgatcgc gtgacgtcgg tattgattgg tgccagccgc
    12181 gcggagcaac tagaggagaa cgtgcaggcg ctgaataatc tgacatttag caccgaggag
    12241 ctggcgcaga ttgatcagca tatcgccgat ggcgagctga atctgtggca ggcgtcgtcc
    12301 gataaatgac ctgttaataa cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg
    12361 acgagtcagt cggtcaagat agcccatcac aaatgcagaa agcacaaacg tcagatggat
    12421 aatgacgtac cacatcagtt tgttatccgg gacatttttc gcatccataa agacgcgcag
    12481 taagtgaatg gaagaaattg ccacaatcga cgctgctact ttgtttttca gcgacgttgc
    12541 gtccattttc cccagccagt tcagcttctc tttgttctcg gagatatcca gctgtgagac
    12601 gaaattctca taaccggaaa acatcaccat caccagcaaa ccgccaacca gcgtcatatc
    12661 caccagcgac agcaacacga ggatcaaatc tgattccgcc atcgagaaga tattcggcag
    12721 tacgtgaata atctcctgga agaacttcag cgccagggca actaacgcca gcgaaaggcc
    12781 aaagtacacg ggggcaagca gccagcgaga agcatacatt gcattttcaa gaaaacgttc
    12841 catacattcc tgtcgtgtag ttaaaacggg caacagtata tcgcaattga gtaaacagca
    12901 cgcaacagca aatcaacata tacgttaaac ctttgtcgca taattctgag gtttagggat
    12961 cttcctggtt cccttatcaa cgcattgaga gcgtctaccc ttaaaggacg ttgaacctga
    13021 agggagaaaa acgatgtctc atttaaaaga cccgaccacg cagtattaca ctggtgaata
    13081 tcccaaacag aaacaaccga cgccaggcat ccaggcgaag atgacaccgg taccggattg
    13141 cggcgagaaa acctatgttg gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg
    13201 gggcgattcc ggaataggtc gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt
    13261 ggcgatcagt tatcttcccg tggaagaaga agacgctcag gatgtgaaaa agatcattga
    13321 agaatgcgga cgcaaagccg ttctgctgcc aggcgattta agcgatgaga aattcgcccg
    13381 ttcgctggtt cacgaagcgc acaaggcgtt aggcgggctg gatattatgg cgctggtcgc
    13441 cgggaaacag gttgccattc cggatattgc agacctcacc agcgaacagt ttcaaaagac
    13501 ctttgccatt aacgttttcg cgctgttctg gctaacccag gaagcgatcc ccctgctacc
    13561 gaaaggtgca agtatcatca ccacttcgtc aatccaggca taccagccaa gtccgcattt
    13621 actggactat gcggctacga aggcggcgat tctgaactac agccgtggcc tggcaaaaca
    13681 ggtcgcggag aaaggtattc gggtgaatat tgtcgcgcca ggccctatct ggacggcact
    13741 gcaaatttcc ggcggacaaa cgcaggataa gatcccgcag tttggtcaga aaacgccgat
    13801 gaagcgtgcg gggcaaccgg cggaactggc ccctgtatat gtttatctgg caagtcagga
    13861 gtcgagctac gtcaccgcag aagtgcacgg cgtgtgcggc ggcgagcatt taggttaaaa
    13921 aaatgcccgg ttgtgaaaag caaccgggca tcattgtgaa ttcactctta cctgatgtgg
    13981 cgaaaacgcc ttatccggcc tacaaacgca cgaagattca acatgttgca atagattttg
    14041 taggcctgat gagcgtagcg catcaggcaa ttctacttta cttcgctttg gcggtttctt
    14101 cgccgaccag acctatcttc aggtaacccg cctgatgcag cgtatccatt accttcatca
    14161 acgtctcgta atcgacggtt ttgtccgctc ggaagaagat ggtggtgtct ttcttgcctt
    14221 cggttaacgc attcaacgcc gtaatcattg tttcatcggt gaccggatcg ttaccgataa
    14281 acatcgagtt gtctgccttc accgacagat aaaccggttt ttccgggcgc ggctgcggcg
    14341 tgctggtaga agcaggcaag ttcaccttca catctaccgt cgctaacggt gccgccacca
    14401 taaagataat cagcagaacc aacatcacgt cgataaacgg cgtcacgttg atatcatgca
    14461 tttcgccgtt atcgtcgagg ttttcgttaa gatgcattgc catcggacat tatcctgcgc
    14521 gtaatttttg tgcgacacga accggatgcg cagcggcgct ggcttccaga tccaggtcac
    14581 ggctttgcag caacaatacc tgcgctgcaa catcacccag catcgcttta aagccgccaa
    14641 tctggcgtgc aaatacgtta tagataacga ccgcaggaat cgctgccacg aggccgattg
    14701 ccgttgctaa cagagcttct gcgatacccg gcgcaacgac tgccaggtta gtggtctgcg
    14761 tttgcgcgat accaataaag ctgttcatga tgccccagac cgtaccaaac agaccaacga
    14821 acggagaaat cgcgccgatg gttgccagat agccgttacc gcggcccatt tgacgaccca
    14881 ctgcggcgac ccgacgctcc agacggaagc tggtacgttc tttaatacct tcgttatcgt
    14941 cgctgccttc tgacagttcc agctcattct gcgcttcatt gagcaaatgc aggcttaagc
    15001 ttttgctacc aaaatcagcg gcgatatcgt tggcctggtt taaggaacgc gcttcagcca
    15061 gcagttgctg ctcgcgctta aggcgacgct tctgattgaa gaactctacg ctcttactga
    15121 agaagattgc ccaagtgact acggaggcca aaataagccc aatcatcacg cacttaacga
    15181 caatatcggc gtgctgatac ataccccaga cggaaaggtc cgtctgcatc aaattattac
    15241 ccactgtgta tctccaggac gcaagtcaca aaatctgcgc ataataatat caaaatgacg
    15301 tcgaattgat agtcgttctc attactattt gcatactgcc gtacctttgc tttcttttcc
    15361 ttgcgtttac gcagtaaaaa agtcaccagc acgccatttg cgaaaatttt ctgctttatg
    15421 ccaattcttc aggatgcgcc cgcgaatatt catgctagtt tagacatcca gacgtataaa
    15481 aacaggaatc ccgagatggc ggacaaaaag cttgatactc aactggtgaa tgcaggacgc
    15541 agcaaaaaat acactctcgg cgcggtaaat agcgtgattc agcgcgcttc ttcgctggtc
    15601 tttgacagtg tggaagccaa aaagcacgcg acgcgcaatc gcgccaatgg cgagttgttc
    15661 tatggacggc gcggaacgtt aacccatttc tccttacaac aagcgatgtg tgaactggaa
    15721 ggtggcgcag gctgcgtgct atttccctgc ggggcggcag cggttgctaa ttccattctt
    15781 gcttttgtcg aacagggcga tcatgtgttg atgaccaaca ccgcctatga accgagtcag
    15841 gatttctgta gcaaaatcct cagcaaactg ggcgtaacga catcgtggtt tgatccgctg
    15901 attggtgccg atatcgttaa gcatctgcag ccaaacacta aaatcgtgtt tctggaatcg
    15961 ccaggctcca tcaccatgga agtccacgac gttccggcga ttgttgccgc cgtacgcagt
    16021 gtggtgccgg atgccatcat tatgatcgac aacacctggg cagccggtgt gctgtttaag
    16081 gcgctggatt ttggcatcga tgtttctatt caagccgcca ccaaatatct ggttgggcat
    16141 tcagatgcga tgattggcac tgccgtgtgc aatgcccgtt gctgggagca gctacgggaa
    16201 aacgcctatc tgatgggcca gatggtcgat gccgataccg cctatataac cagccgtggc
    16261 ctgcgcacat taggtgtgcg tttgcgtcaa catcatgaaa gcagtctgaa agtggctgaa
    16321 tggctggcag aacatccgca agtagcgcga gttaaccacc ctgctctgcc tggcagtaaa
    16381 ggccacgaat tctggaaacg agactttaca ggcagcagcg ggctattttc ctttgtgctt
    16441 aagaaaaaac tcagtaatga agagctggcg aactatctgg ataacttcag tttattcagc
    16501 atggcctact cgtggggcgg gtatgaatcg ttgatcctgg cgaatcaacc agaacatatc
    16561 gccgccattc gcccacaagg cgagatcgat tttagcggga ccttgattcg cctgcatatt
    16621 ggtctggaag atgtcgacga tctgattgcc gatctggacg ccggttttgc gcgaattgta
    16681 taacattgcc acttttggac aattttgcag acatttaatt gtgaaaagtc ttaaattgtt
    16741 gcgtccggga tcaaggcgtc ccggacgaat caggagtaca ataggcagat aaaggcttaa
    16801 acgctgttcc acaggaaagt ccatggctgt tattcaagat atcatcgctg cgctctggca
    16861 acacgacttt gccgcgctgg cggatcctca tattgttagc gttgtttact ttgtcatgtt
    16921 tgccacgctg tttttagaaa acggcctgct gcccgcctca tttttgccag gcgacagctt
    16981 gttgatactg gcaggcgcat tgattgccca aggggttatg gattttctgc ctacgattgc
    17041 gattctgacc gccgcagcaa gtctgggctg ctggctaagt tatattcagg ggcgctggtt
    17101 agggaatacc aaaacggtga aaggctggct ggcacagctt cctgctaaat atcaccagcg
    17161 cgccacctgc atgtttgacc gccacggtct gctggcgctg ctggctggac gttttcttgc
    17221 atttgtccgt acgctgctgc caaccatggc gggaatttcc ggtctgccaa accgccgctt
    17281 ccagtttttc aactggttaa gtggattgct gtgggtcagc gtggtaacca gttttggcta
    17341 tgccttaagt atgattccgt tcgttaaacg ccatgaagat caggtaatga cgttcctgat
    17401 gatcctgcca attgccttgt taaccgctgg cttgttaggc acgctgtttg tagtgattaa
    17461 aaaaaaatac tgtaacgcct gacgattttc cccgttcccg gctgctgtac cgggaacgta
    17521 tttaattccc ctgcatcgcc cgcattcttg ccgcatcttc ccccggcgtc acaccgaagt
    17581 aacgtttaaa ctcacggcta aattgcaatg cgctttcata gtcgacgcgc atcgctgctg
    17641 cgctggcctt catgccgtca tggatgatca tcatccgcgc cttatgcaga cggtaattct
    17701 tcaaatactg caacggcgag gtgctggtga cagacttaaa attatgatgg aacgccgata
    17761 cgctcatgtt ggcttctgcc gccagttgct cgacgctcag gttttcggtg tatttattct
    17821 caatccgttt cagcacgcgg ctaatcagac tgaagtgagt ctggcgactg accagcgcca
    17881 gtaacgcgcc gccgcaaggt ccggtcagca cgtagtacag aatttcgcgg atgatctgtt
    17941 tgccgagaat acgcgcatcc agtggtcgct ccatcacgtc gagtaaccgc tccgccgcgc
    18001 ataaaatctc ttctgataac gtggcggagt taatcccgct ggctgccatc gacggctgga
    18061 aatgctcatc ttcgccaatg tccatcaaca gttcctgtaa ctgcaaaata tcgacattga
    18121 gacgcaatcc tgccagcggc acctctgacg tggcataggt ttcgcactca aacggcaacg
    18181 gcaccgtcag cagcaggtat tcattggcat cataacgaaa cacgcgttca ttgatataac
    18241 cgattttatg cccggaaaag agaattatga tgccaggctc gtacatcacc ggtgtacgtg
    18301 cgaaaggcgt ctcgccatac aacaaacgca catcgggcaa cagttctgac aaactatttt
    18361 ctttattttt cagtttatta actttatccg ccagcaagcg gcaaatctct tcacgtttca
    18421 tatcgcgtaa tttcttagga ataatgcggc aatttgattg tgcgcaattt tgtagcattt
    18481 ctccagcact ctggagaaat aggcaagaca ttggcagaaa tgagcattga gagccagggc
    18541 gctggcgatc acaatgaaaa acatcaggca gatcgttctc tgccctcata ttggcccagc
    18601 aaagggagca agtaatgaac aactttaatc tgcacacccc aacccgcatt ctgtttggta
    18661 aaggcgcaat cgctggttta cgcgaacaaa ttcctcacga tgctcgcgta ttgattacct
    18721 acggcggcgg cagcgtgaaa aaaatcggcg ttctcgatca agttctggat gccctgaaag
    18781 gcatggacgt gctggaattt ggcggtattg agccaaaccc ggcttatgaa acgctgatga
    18841 acgccgtgaa actggttcgc gaacagaaag tgactttcct gctggcggtt ggcggcggtt
    18901 ctgtactgga cggcaccaaa tttatcgccg cagcggctaa ctatccggaa aatatcgatc
    18961 cgtggcacat tctgcaaacg ggcggtaaag agattaaaag cgccatcccg atgggctgtg
    19021 tgctgacgct gccagcaacc ggttcagaat ccaacgcagg cgcggtgatc tcccgtaaaa
    19081 ccacaggcga caagcaggcg ttccattctg cccatgttca gccggtattt gccgtgctcg
    19141 atccggttta tacctacacc ctgccgccgc gtcaggtggc taacggcgta gtggacgcct
    19201 ttgtacacac cgtggaacag tatgttacca aaccggttga tgccaaaatt caggaccgtt
    19261 tcgcagaagg cattttgctg acgctaatcg aagatggtcc gaaagccctg aaagagccag
    19321 aaaactacga tgtgcgcgcc aacgtcatgt gggcggcgac tcaggcgctg aacggtttga
    19381 ttggcgctgg cgtaccgcag gactgggcaa cgcatatgct gggccacgaa ctgactgcga
    19441 tgcacggtct ggatcacgcg caaacactgg ctatcgtcct gcctgcactg tggaatgaaa
    19501 aacgcgagac caagcgcgct aagctgctgc aatatgctga acgcgtctgg aacatcactg
    19561 aaggttccga cgatgagcgt attgacgccg cgattgccgc aacccgcaat ttctttgagc
    19621 aattaggcgt gccgacccac ctctccgact acggtctgga cggcagctcc atcccggctt
    19681 tgctgaaaaa actggaagag cacggcatga cccaactggg cgaaaatcat gacattacgt
    19741 tggatgtcag ccgccgtata tacgaagccg cccgctaagc tttttacgcc tcaaactttc
    19801 gttttcgggc atttcgtcca gacttaagtt cacaacacct caccggagcc tgctccggtg
    19861 agttcatata aaggaggaac gtatggctaa tccaaccgtt attaagctac aggatggcaa
    19921 tgtcatgccc cagctgggac tgggcgtctg gcaagcaagt aatgaggaag taatcaccgc
    19981 cattcaaaaa gcgttagaag tgggttatcg ctcgattgat accgccgcgg cctacaagaa
    20041 cgaagaaggt gtcggcaaag ccctgaaaaa tgcctcagtc aacagagaag aactgttcat
    20101 caccactaag ctgtggaacg acgaccacaa gcgcccccgc gaagccctgc tcgacagcct
    20161 gaaaaaactc cagcttgatt atatcgacct ctacttaatg cactggcccg ttcccgctat
    20221 cgaccattat gtcgaagcat ggaaaggcat gatcgaattg caaaaagagg gattaatcaa
    20281 aagcatcggc gtgtgcaact tccagatcca tcacctgcaa cgcctgattg atgaaactgg
    20341 cgtgacgcct gtgataaacc agatcgaact tcatccgctg atgcaacaac gccagctaca
    20401 cgcctggaac gcgacacaca aaatccagac cgaatcctgg agcccattag cgcaaggagg
    20461 gaaaggcgtt ttcgatcaga aagtcattcg cgatctggca gataaatacg gcaaagcccc
    20521 ggcgcagatt gttatccgct ggcatctgga tagcggcctg gtggtgatcc cgaaatcggt
    20581 cacaccttca cgtattgccg aaaactttga tgtctgggat ttccgtctcg acaaagacga
    20641 actcggcgaa attgcaaaac tcgatcaggg caagcgtctc ggtcccgatc ctgaccagtt
    20701 cggcggctaa catgcaaatt ctcccggtga cggtaatgtt ccgctaccgg acttttcaga
    20761 aatcatttat tcccctcgcg tcccgcccgt tgttactctt ccttgttcag gaatgccaaa
    20821 tataaggaca tcatcatgca gagccggaag ctcttaaaag aacaactcat ctatatccgg
    20881 gataaacgca acggagaggt gaaaaacaga tgaaaataat acttctgttt ttagcagccc
    20941 tggcaagttt taccgtacac gcgcagcctc cttcacagac cgtagaacaa acagtccggc
    21001 agatttatca gaactataaa tcagatgcca gtacacctta tttcggtgaa accggagagc
    21061 gggcgataac ttcagcgcgt attcaacagg cgcttaccct gaacgacaat cttacgctgc
    21121 cgggcaatat tggctggctg gattatgatc cggtttgtga ttgtcaggat tttggcgatc
    21181 tggtgctaga aagcgttgcg ataacccaaa ctgacgccga tcatgccgat gccgttgtgc
    21241 gctttcgtat ctttaaagat gataaagaaa agaccacgca gacactgaaa atggtggcgg
    21301 aaaatggtcg ttgggtcatt gacgatattg tcagcaatca tggcagcgtc ttacaagcag
    21361 ttaatagcga gaatgaaaaa acgctggccg ctttagcttc gttgcaaaaa gaacagccgg
    21421 aagcctttgt tgccgaactc tttgaacata ttgctgatta tagctggccg tggacgtggg
    21481 tggtttccga ctcttaccgc caggcggtta atgccttcta taaaaccacc ttcaagacgg
    21541 ccaataatcc cgatgaagat atgcaaatag aacggcaatt tatttacgac aatccgatct
    21601 gttttggcga agagtcgcta ttttcacgcg ttgatgaaat tcgagtcctg gagaaaaccg
    21661 ccgattccgc ccgcattcat gttcgtttta cgctgaccaa tggcaacaac gaagagcaag
    21721 aactggtttt acagcggcgc gaaggcaagt gggaaatcgc tgattttatc cgcccgaaca
    21781 gcggcagcct acttaagcag attgaggcaa aaaccgccgc cagattaaag caatgagctg
    21841 aattaaataa caattagccg gaacaataaa taaaagggaa cactatatga aaacgatttt
    21901 caccgtggga gctgttgttc tggcaacctg cttgctcagt ggctgcgtca atgagcaaaa
    21961 ggtcaatcaa ctggcgagca atgtacaaac attaaatgcc aaaatcgccc ggcttgagca
    22021 ggatataaaa gcactacgcc cacaaatcta tgctgccaaa tccgaagcta acagagccaa
    22081 tacgcgtctt gatgctcagg actattttga ttgcctgcgc tgcttgcgta tgtacgcaga
    22141 atgataaaaa aatccccggc agcatgtcag ttgccgggga ttttttttaa cgtccaaccg
    22201 ccgctttagg gcgtttcttc gcaccagcat tcaccggacg agattgcgta gacgacgctt
    22261 tttttgccgt agcaggcgtc tgacgctggg tcgccatcgg cgtatgttta gtcaacgccg
    22321 gacgggtatt gcggttctgg cgacgagctt cacgcatctc ttcaatggtt ggcgcaggca
    22381 ctaagcaatc gcgacggctg ccaatcagat gctttttgcc catcgcttcc agcgccgggc
    22441 ggattaacgg ccagtttgcc ggatcgtggt aacgcaacaa cgctttatgc aaacgacgct
    22501 gtttgtcgcc cttcggtacg aagacgtctt cgctcttata accaatcttc gccagcggtt
    22561 ttttcccggt gtaatacatg gtggttgagt tcgccagcgg cgatgggtag aagttctgta
    22621 cctggtcgag acggaaacga tgctttttca gccacagcgc caaattcacc atatcttcat
    22681 cacgcgtacc ggggtgcgca gagatgaaat acgggatcag atactgctct ttacctgcct
    22741 gtttcgagta ggtatcgaac agctctttaa agcggtcata gctgcccatg cccggcttca
    22801 tcatcttcga caacggccct tcttcggtat gttccggagc aatcttcaga taaccgccga
    22861 cgtgatgggt cgccaactct ttgatatagc gcggatcttc cacggcgatg tcataacgta
    22921 cgcctgaggc gatgaggatt tttttaatgc ctttcagatc acgcgcacgg cgatagaggt
    22981 tgatcgtcgg ttcgtggtta gtgtccatgt gcggacaaat atccggataa acgcacgaca
    23041 aacggcgaca agtttgttca gcgcgtggcg atttgcagcg caacatatac atgttggcag
    23101 ttggcccacc gagatcggaa atcacgcccg taaaacctgg aacggtgtcg cggatcgctt
    23161 cgatctcatt aatgatcgaa tcttccgaac ggctctgaat aatgcgccct tcgtgctcgg
    23221 taatagaaca gaaagaacag ccgccaaagc agccgcgcat aatgttgacc gaaaaacgga
    23281 tcatttcgta agccggaata cgggcattgc cataggccgg atgtggcacg cgcttgtacg
    23341 gcagcgcaaa aacgctgtcc atctcttcgg tagaaagcgg gatagcaggc gggttgatcc
    23401 acacatagcg gtcaccgtgt ttttgcatca atgcgcggac gcagcctggg ttggtttcgt
    23461 ggtgcagaat acgcgaagca tgggcgtaca gcactttatc gcccttcact ttctcgaaag
    23521 aaggcagcaa cacgtaggtt ttttcccacg gtttcgggcg cggtggctgc acggttacgg
    23581 ctttggcttc ctgctttttc ggtgccaccg gtttgttatc cgcgcacggc aaatcttcac
    23641 catacggatg cgggattggg tcgatttttc caggggtatc aagacgggtg gaatccacgc
    23701 cgctccagcc aggcagcgcc tctttcacga taatcgcggt attacgcaca tcgcggattt
    23761 cactaatcgg ctcgcccatc gccagacggt gcgccacctc caccagcgga cgctcaccgt
    23821 taccaaacat cagcatgtcg gctttcgaat ccaccagcac ggaacggcgc acggtatcgg
    23881 accagtaatc ataatgtgcg gtacggcgca gactagcctc aataccgccg aggatcaccg
    23941 gtacatcttt ccacgcctct ttacaacgct gggtataaac cagtgtggcg cgatccgggc
    24001 gcttacccgc gacgttatcc ggcgtgtagg catcgtcatg acgtaaacgg cgatcggcgg
    24061 tataacggtt gatcatcgaa tccatgttgc cagcagtaac accgaaaaac agattcggtt
    24121 tacccagacg cataaagtcg tctttgctgc tccagtccgg ctgggcgatg atcccgacgc
    24181 gaaagccctg tgcttccagc atacgaccgc aaatcgccat cccgaagctt gggtgatcga
    24241 catacgcgtc gccagtaacc aaaatgatgt cgcagctatc ccagccaagt tgatccatct
    24301 cttcacgaga catcggcaaa aacggtgccg gtccaaaaca ggcggcccag tactgcggcc
    24361 aggagaacag gtcgcgatcc ggttggatca gggagataga gctcattttg cttccagaaa
    24421 tgataaaaaa ataatcaaag gccggggatt ataagccgga acgaaagaga aatcgaaaag
    24481 tattccatac tcgccctcct cgggcgagta tgaagattac ggtaccggat tgactaacag
    24541 ttgcccaatc gagccacggt ccgccatttc cagcgtctga ctgttgaagt agaacgggaa
    24601 gtgcgcccag gaaggctgac cgaaataaac aagcagctcc acttgtccgt caacccaaac
    24661 ggtatctttc cagcctctgt cttccgggaa cggcatcgca ccattcacgt tacggatctg
    24721 gaacatcacg ccttcaatat ggaacgcttg cggctcgtcc gcgcgtaccg tccagcgttc
    24781 ccacgttcct tgctgcgcgg tgacatcaat acggttgacg tcccacagct gtccattaat
    24841 acccgggtca tcacccagac tgatatcgcg actgcgaatc ggcgaaccgg ccatgatttc
    24901 agttggcagc aagcgcatcg gaagactgtc tgtgaccagc ggcagaaggc cggttgggcg
    24961 cagcgttagc accagggtag aaaccagaat actggatggc tcaaagaagc cgcgaatacg
    25021 atcaacaatg ctcgccgctt cgccacaggt gatcgacact tcatcgccgt tgctcatatc
    25081 caccagaatc tcgcggcgct cgcccggtgc cagcgaaagt tgcttcaccg atacaggagc
    25141 aggcaggaat ccctgatcgc cagaaatcac atgtaacggg cgaccatcgc tcatctgtag
    25201 ttgatagcga cgagagttcg acgcgttcag cagtcgcaag cgcacccagc cacgcgagac
    25261 ttcaacgtac gggctttgta caccgttaac cagcagcgta tcaccaacaa agccgccgct
    25321 tcccggttcg ttgtattctg gcgtaccaaa gttatccagc cgtttatcct ggataatgac
    25381 cggaaaatca tccacatcat aatggttggg gataggcagc gacttgctga cttcatcttc
    25441 caccagccac attccggcaa ggccgttata gacctgctga gccgtgcggt tgggagtatt
    25501 ggcgtgatac cacagagtag ctgcgttctg gcgaataggc agtacgggtg cccagtcagc
    25561 gtttggcgac atcatccgtg ccggaccgcc catcagcggg ccaggtacct gtagcccggc
    25621 caccgtcatt gagacatttt ctgtcaggcg gttgctgtaa ataagcttaa catcgtcgcc
    25681 cttccagacg cggatagtcg gccccaggta acgaccattg attccccaga ccgacgcgcg
    25741 tgtccctggc gtaaatgacc agtgcgcacg ttgtacagtc ataaacagcg gttgcccacg
    25801 gcgagattca agtagcggcg gaacgggtag cggttgctgt tgcccggctg cgctggcctt
    25861 caggggaaca gcgcctgcac aaagtgcaat ccccgatgcc tgaatgaact gacgccgact
    25921 gagtgacata tttgctccat gtaaaactgg ctaaagtaat accgtgcagg aaagtgttcc
    25981 ccgcaaaaat acagattgcc gacttaaact tttccggcgg cttcgcgttc tgcgacttct
    26041 ttatcgagct cggcgatttt ttgttccatt atcgaacgac aatgggcagc cagctcacga
    26101 acctgatctt tgccatactg actgacgtca attggcggca gcatttcgac aatcaccaga
    26161 ccgttgtgca gtcgattaag attaatctta ttcgaagttg tagagacgca cacgggaata
    26221 atcgggacgc ccgccgcaat tgccgcgtga aatgctccag tcttgaacgg tagcaggccg
    26281 cgaccacggc tgcgggttcc ttccgggaac atccagatgg aaatacggcg ttttttgaaa
    26341 tgattcacga cttccgcaat ggtgccgtga gctttagtgc gattgtttct gtcgatcaat
    26401 aagttgccgg ttaaccagta caactgcccg aagaagggga tccacagcaa gctcttttta
    26461 cctaccgtca ccgtcggcgg ttgcacaatg ttcgatgctg tcaccatgtc atagttgttc
    26521 tggtggttag cgatatagat agcattgccg tagctttcag catctgcagg tttgcggcac
    26581 tcaactttca ggccgaacag cggcgcaaga cggccaaaca tatgcccaaa ggtggccaca
    26641 tgtttcgggt tacgcgggct gaaaaggcag taaatggagc cgaatacaca gactaagatg
    26701 ctgtaaatca cggtaataat aagacgaaag atatatagca taacaccctc tgaaggtctg
    26761 agagactggc attgtacgtc acctgaaatc aggttagggc attgttaaaa gccgctctct
    26821 ggaaaagcgt attgttaatc gcaacgcatg aataaacaac ggtttcaagg gaatttttat
    26881 cgtttccggg ggttgaatac gctgccggat gacgacttaa cgtttcatcc ggcgttcctt
    26941 gcaagcggga ggaaacaggc cctccccggc atattactct tcgctatcac cgttgctggc
    27001 acggcgagga gagtcgatct caacacgatc gatacgctgc aaaccgcgca tcaacgtacc
    27061 gcggcgtcca cgttcaccag tgactttctg taactcttcc gggcgcagtt taattttgcg
    27121 tttcccaaca tgaatggtca gcgtgctttg cggcggcaga acgtacaatt gcgccagacc
    27181 atcttctcca cgcgcggctt ctgccgatgg aatgttgata atcttgttgc ctttgccctt
    27241 cgacagctgc ggcagatcgc ttaccgggaa catcaacata cggcctgcct gagtgattgc
    27301 cagcagcata tcggaagcat cttcaatcac caccggcggc ataacatggg cattttccgg
    27361 taaggtgatc aaagccttac ctgcacggtt acgcgccacc agatcgttaa aggtgcagac
    27421 gaaaccgtaa cccgcatcgg aagccatcag cagtttctga tcgtcgcttt ccatcagcat
    27481 atggtcaacg gtcgccccag gcggcaacgt taatttgccg gtgagtggtt cgccctgacc
    27541 acgcgccgac ggcagcgtaa tcgggtcgat ggcatagcta cgaccggtgg aatcaacaaa
    27601 cactaccgat tggttgctct tacctttcac cgccgctttg aagctatcac ccgctttata
    27661 attcaggccc ggcgcgtcga tatcatggcc tttagcgctg cgtacccagc ccatctgcga
    27721 cagcacaatg gtgacaggtt cagacggcag catgtcgtgc tcgctcatcg ctttcgcttc
    27781 ttcgcgttcc tgcaacggcg aacgacgatc gtcaccgtag gcttgcgcgt ctgcctgcag
    27841 ttctttcttc agcaggttat tcattttacg ctcggaagcc aaaatgccct gcaactggtc
    27901 gcgctctttt tccagctcac tctgctcacc gcgaatcttc atctcttcca gtttggcaag
    27961 atgacgcagt ttcagttcga ggatcgcttc cgcctgggtt tccgtaaggc caaaccgcga
    28021 catcagcgcc ggtttcggtt catcttcgtt acgaatgatc tcaatcactt cgtcgatgtt
    28081 gagaaacgcc accagcaaac cttccaggat atgcaggcgc ttgaggactt tctccagacg
    28141 atagttcagt cggcggcgca cggtatcgcg acggaacacc agccattcgg agaggatttc
    28201 cagcaggttt ttcaccgccg gacgaccatc cagaccgatc atattgaggt taatgcgata
    28261 gctcttttcc agatcggtgg tagcgaagag gtggttcatc acctgatcca tatccacgcg
    28321 gttggaacgc ggcacaatca ccagacgggt cgggttctcg tggtcagatt catcgcgcag
    28381 atcgtcaacc atcggcagct ttttgttgcg catttgcgca gcaatttgct ccagtacacg
    28441 cgcacctgaa acctgatgcg gtaatgcgct gataaccacc gcgccatctt ctttcttcca
    28501 caccgcgcgc atacgcactg aaccacgtcc gttctcgtag attttacgga tctcggcgcg
    28561 cgaagtgata atttccgctt cagtcggata atccggcccc tgcacgatat ccagcagctg
    28621 atcgagcgtg gttttcggct ggtcgattaa tgcgattgcc gcctgagcta cttcacgcag
    28681 gttatgcggt ggaatatcgg tcgccatgcc gacggcaata ccggtggtgc cgttaagcaa
    28741 aatgtttggc agacgagcag gcagcatttt cggctcctgc aaagtgccgt cgaagtttgg
    28801 cacccagtca gccgtcccct gccccagctc gctcaatagc agctcggaat atttcgacaa
    28861 ccgggattcg gtgtaacgca ttgccgcgaa cgatttcgga tcgtccggcg cgccccagtt
    28921 cccctgacca tcaaccagcg gataacggta agagaacggc tgcgccatca ggaccatcgc
    28981 ttcataacag gcgctatcgc cgtgcggatg gtatttaccc agtacgtcac cgacggtacg
    29041 ggccgatttt ttaaatttgg cgctggcatt caggcccagt tcagacatcg catacacaat
    29101 gcggcgctga acaggtttca gaccatcacc aataaacggc aacgcacggt ccatgatcac
    29161 gtacatggag tagtttaagt aggcgttttc cgtaaattca tgtagcgcaa ggcgctctgc
    29221 catatcgctc attaattctg attcctcaac tgattcgcca gccttcaaag gggcaatatt
    29281 gccggagata ctacattatc tgccgcgtcg agtcacagga gatcagcgtg aatagcgccg
    29341 ccggacgtgc tccgacggcg ataaaggcat caatgtgcct tgatatacaa ctctttgctg
    29401 tagacttgat cggcatagag ggcagcacaa ggttgcgccc atgccacacc actcggcatg
    29461 ggggtccgct ccgggcgatt cgagaagttg aggttatgcg agtccaggga agcctaaagc
    29521 atcaaagtag tcgactgtaa gtatatggtt cagcccagtt tagctgtgtc tccaccagtg
    29581 ccccgcgcct cctgccataa gtgcggcttg ctcatgggta cccccgagac ggcttacggc
    29641 gcaccggata ataaatttcg cgtatacatg gtattcctta ccggagaaat tataagccca
    29701 acaacaatac gcctgtggca ttgttcgctc aaacttatcg cgagtgattt ggctcacact
    29761 ccggtaaaga ttccccatca cgctaacggc acataaacat ccgtttccag tcgctgctcc
    29821 gtcacccctt cggcaagatt ggtgtagtga aacagaatcg gtgctttacg cattttctcg
    29881 ccgcttgcag gcagccagtg gcgaataatg ccccatatcg tgtgtgaaat atcgtccagc
    29941 tcgccaacgt ggcgggccac ggcataacgt ccaccggtaa gttcaccatt gctcacacca
    30001 taacgattat cgggaatcgg ttcgctaacg ctgccgcaga tatcaaagcg aaacgcttcc
    30061 ggtgcggtgg tggcagggtc gtcccaggcg acgccaaaag tctggctttg gttaacagga
    30121 gaaagccccg tctctttacg ccacatgata aatttcgcca cactgtagtt gagcaactca
    30181 gggctacagc ggtgcggcaa catcgccaca gggattgatg ggaaatcgat aatgtttaca
    30241 tccagtgtca ggtttgtcat tttgcctccc tgcgaaatta ttcccgggaa cgcgtcccgg
    30301 gagcggtaac aattacggat ttactttgcg gatctgtttg acgtcaattt caacagaatt
    30361 ccagtcttta tcgacttcac cctgaatctc aaccgtatct ttcggcgtca ccgtcacacc
    30421 gttccagcgt ttgtggtcga tatcaacatt gatagtaccg ctggcatctt taaacacgta
    30481 gagatcgtca gagatgcgtt caacgatatt gccgcgcagg gttacccagg tgtcgtcacg
    30541 cagggatttt gcgctttcta cagtcgttac gctgccgttc ggcccctgga atcctccggc
    30601 ctgactttgc gttgccgatg ggccagaaaa accgccctgc tctgctgcca tcaccggtgc
    30661 actgcacagg gccattactg cgattactgc tgcgaatttt ttcatgttta ttactccctt
    30721 taatgtctgt ttccgagcat ttaacaagat agtccttaac aacttcttaa gggaaaaaaa
    30781 taaaatttaa tgctgtacag agcgcgttac aacacggttt actggcagca aatacggtta
    30841 tcgcagggat gaaaaaatgc gaattttact gatagaagat gacatgctga ttggcgacgg
    30901 catcaaaact ggccttagta aaatgggttt tagtgtcgac tggtttacac aaggtcgtca
    30961 gggaaaagag gcgctttata gcgcacctta tgatgcggtg atcctggatt taaccttacc
    31021 aggcatggat ggtcgcgata ttttgcgcga atggcgagag aaaggtcagc gtgagccggt
    31081 actgatcctg accgcgcgtg atgcgttagc ggaacgtgta gaagggctgc gtctgggagc
    31141 tgacgattat ctgtgtaaac cttttgcgtt gatagaagtc gccgccaggc tggaggctct
    31201 gatgcgccga accaacggcc aggccagcaa cgagctgcgc cacggtaacg tcatgctcga
    31261 ccccggcaaa cgtatcgcca cgctggctgg cgaaccctta acactgaaac caaaagaatt
    31321 tgccctgctg gaattactga tgcgtaacgc tggtcgggta ctgccgcgca aactgattga
    31381 agagaaactg tatacctggg acgaagaggt caccagtaat gccgttgaag tgcatgtgca
    31441 tcatctgcga cgcaaactcg gtagtgattt tattcgtacc gtgcatggta ttggctacac
    31501 attaggtgag aaatgaaatt tacccaacgt cttagtctgc gcgtcaggct gacgctaatc
    31561 tttttaattc tggcctcggt gacctggctg ctttccagct ttgtcgcctg gaaacaaaca
    31621 acggataacg tcgatgaatt gttcgacacc caactgatgc tgtttgccaa gcggttaagt
    31681 acgctcgatc tcaacgaaat caacgcggcg gatcgcatgg cacagacgcc aaataaatta
    31741 aaacacggtc atgttgatga cgatgcgctg acctttgcca tctttaccca cgacggcaga
    31801 atggtcctta atgatggcga taacggagaa gatattccct atagctatca acgggaaggt
    31861 tttgctgacg ggcaactggt cggtgaagac gatccttggc gttttgtctg gatgacctca
    31921 cctgatggca aatatcgcat cgttgttggc caggaatggg aataccgtga agacatggcg
    31981 ctggcgattg ttgccgggca attgatcccg tggctggtcg cactgccgat tatgttaatc
    32041 atcatgatgg tactactggg tcgtgaactc gcgccgctga acaaactggc gctggcacta
    32101 cgtatgcgtg accctgactc ggaaaaacca ctaaacgcga ctggcgtacc cagcgaagtg
    32161 cgaccactgg ttgagtcgct aaatcaactg ttcgcccgca cacatgcgat gatggttcga
    32221 gaacgacgct ttacctccga tgcagctcac gaactacgta gcccgttaac agcgctgaaa
    32281 gtgcaaaccg aagttgcgca gctctctgac gatgatccgc aggcgcggaa aaaagcactg
    32341 ctccaattac attccgggat cgatcgcgct actcgtctgg ttgatcaact gctcacgcta
    32401 tcgcggctgg actcactgga taaccttcag gacgtcgcgg agatcccgct tgaagatctc
    32461 ctgcaatcgt cggtgatgga tatttaccac acggcgcagc aggcgaaaat tgacgtgcga
    32521 ctgacactca atgcccacag catcaaacgc actggacaac cgctattgct aagtttgttg
    32581 gtgcgaaacc ttctggataa cgccgtgcgc tacagtccac agggcagcgt ggtggacgtc
    32641 acgctgaatg ccgataattt catcgtgagg gataacggcc ccggtgtgac accagaggca
    32701 ctggcgcgaa ttggcgaacg cttctatcgc ccccccggac aaaccgctac cggcagtgga
    32761 cttgggctat cgattgtcca gcgaatcgcc aaattgcatg gcatgaatgt tgaatttggg
    32821 aatgcggaac aaggtggatt tgaggcgaag gtgagctggt aagggtaaga cttttgctaa
    32881 attcgcaaaa gactttgcac attttgctaa ttttaccgta ccgctctgtg acgtactata
    32941 gtcgacaaac gtctcacctt gaggttaaaa aatgagcaac accctgatta tcaacggcgc
    33001 gaaaaaattc gcccactcca atggtcaact gaacgacacc ctgaccgaag tcgcggatgg
    33061 cacactgcgc gacctcgggc atgatgttcg catcgttcgc gccgacagcg actacgatgt
    33121 caaagcggaa gtacaaaact ttctctgggc tgatgtggtg atctggcaga tgccaggctg
    33181 gtggatgggc gcgccgtgga cagtgaaaaa atacattgat gatgtattca ccgaaggtca
    33241 cgggacgctg tatgccagcg atggccgtac ccgcaaagat ccgtcaaaaa aatacggttc
    33301 cggcggcctg gtacagggca aaaaatatat gctttctctg acctggaacg caccaatgga
    33361 agccttcacc gaaaaagatc aattcttcca cggcgttggc gtcgacggtg tgtatctgcc
    33421 gttccataaa gcaaaccaat tccttggcat ggaaccgctg ccgacattta tcgctaatga
    33481 cgtgataaaa atgcctgatg ttccccgcta tactgaagaa tatcgcaagc atcttgtgga
    33541 aatttttggt taactagagc tcaggcttta gaaggagtta accatgctta ccgtaatcgc
    33601 agaaatccgt actcgtcctg gtcaacatca ccgtcaggcg gtattggatc agtttgctaa
    33661 aatcgttcca accgtactga aagaagaagg ttgccacggc tatgcgccaa tggtggattg
    33721 cgctgctggc gtgagtttcc agtctatggc accggattct atcgtgatga ttgagcagtg
    33781 ggaaagcatc gcgcaccttg aagcgcatct gcaaaccccg cacatgaagg cgtacagcga
    33841 agccgtaaaa ggcgacgtgc tggagatgaa tattcgtatt ctgcagccag ggatttaatc
    33901 ctgccttgtt tgcccggcca tcctgaccgg gcaatgttct ttcctttaaa cctcaatctc
    33961 cgccatgtcg cctttctctt gcaaccagtt gcggcgatct tccgagcgtt tcttcgccag
    34021 cagcatatcc atcatcgcgt cagtacgctg atcgtcttca tcatcgatag tcaactgcac
    34081 cagacggcga gtgttcggat caagcgtggt ttcacgcaat tgcatcgggt tcatttcccc
    34141 cagaccttta aaacgctgga cgttcggctt gcctttcttg cgttttaatt gctcaagtac
    34201 gccctctttc tcttcttccg tcagcgcgta ataaacctct ttcccgagat caatacggta
    34261 gagcggtggc agtgcgacgt aaacgtgacc gtgtttcacc aacgcgcgga agtgttttac
    34321 gaacaaagcg cagagcagcg tggcaatgtg cagaccatca gagtccgcat ccgcgaggat
    34381 acagattttg ccataacgaa gctggctcag atcgtcgctg tcaggatcga taccgatcgc
    34441 taccgaaata tcatgcactt cctgcgaggc cagcacttcg tcggaagaaa cttcccaggt
    34501 gttaaggatc ttacctttca gcggcatgat cgcctgatat tcacgatcgc gcgcctgctt
    34561 ggcagatccg cctgcggagt caccttccac gaggaacagt tcggtacggt taaggtcctg
    34621 cgcggtacaa tcagccagtt tgccaggcag cgccgggccg ctggtcagct ttttacgcac
    34681 cactttttta gccgcacgca tacgacgctg ggcgctggaa atcgccatct ctgccagcaa
    34741 ctccgccgcc tgaacgttct ggttcagcca caggataaag gcatctttca ccacgccaga
    34801 aacgaatgcc gcgcattgac gcgaagagag acgctctttc gtctgcccgg caaactgcgg
    34861 atcctgcatt ttcaccgaca gaacataggc gcagcgatcc cagatatctt ccgccgacag
    34921 cttcacgccg cgcggcagaa tgttgcgata ttcacagaac tcacgcatcg cgtccaacag
    34981 gccctgacgc aggccattaa catgggtacc gccctgcatc gttgggatca ggttgacgta
    35041 gctttcggtc agcagttcac cgccttccgg cagccacagt agcgcccagt ccacagcttc
    35101 agtatcgcca gcgaaattac cgataaacgg tttttctggc agcgtcggta aaccgtttac
    35161 cgcttccgcc aggtaatcat tcagaccgtc ctgatagcac cagcgctgtt cggtgttgtt
    35221 gatctcatct ttaaaggtaa tctcaacgcc ggggcacagt accgctttgg ctttcagcac
    35281 atgcgtcagg cgtgaaacag aaaatcgcgg gctgtcaaag aaggtttcat ccggccagaa
    35341 gtgcacgctg gtaccggtat tgcgtttacc gcaagtgccg acaacctgta aatcctgcac
    35401 cttttcgcca ttttcaaagg cgatgttata aacctgacca tcgcggcgca cgttaacttc
    35461 aacgcgcttc gacagggcgt taaccaccga aatccccacg ccatgcaggc cgccagagaa
    35521 ctggtaattt ttgttagaga atttaccgcc tgcatgcaga cggcaaagaa tcaattcaac
    35581 cgccgggaca ccctcttccg ggtgaatatc caccggcatc ccgcgcccat cgtcaataac
    35641 ttctaacgac tggtcagcat gtaagattac atccacgcgt tttgcgtgac ccgccagtgc
    35701 ttcatcgaca ctgttatcaa taacttcttg ccccaaatgg ttagggcgag tggtatcggt
    35761 atacatcccc ggacggcggc gaaccggctc aagcccggtg agtacctcaa tggcatcagc
    35821 gttataagtt tgcgtcatgg tttaagttag caattcgagt tgatcgtcag agatggtgca
    35881 gaccaagaaa atcgacgatc gggttgaaat aatcttcgaa gcccgtgaat gcgtggttgc
    35941 cgccttctat gacagtctgg cggcaggaag cgtagtacgc caccgcctgg cggtaatcca
    36001 gcacttcatc tcccgtctgt tgcagcagcc agatcaaatc cggcgcttcc agcgggtcaa
    36061 tctgcatgac tttgagatcg taaatatggc gtgactctag cacatattgc tgcccggtgt
    36121 atgggttctc gttctgaccg agatagtccg tcagcagttc aaacgggcgc accgccgggt
    36181 ttaccaccac tgcgggcagc ataaaacatt gtgacaacca ggtggcgtaa tatcccccca
    36241 gtgacgaacc gacaataccc agcgaatcgc cgccatgttc caggacaatg gattccagca
    36301 gttctgccgc gtcggaagga tacggcggca actgcggaat gatcatctca acgtcagggt
    36361 gatgttccgc cagccagttt tttaacaagc tcgcttttgc agagcgcggc gagctgttga
    36421 aaccgtgtaa ataaagaagc gtagacatca gtagccttct gaagcggtat caggttggaa
    36481 acgtgtgtcc gccaggcgat gcacctcggt ggtcagcgtg ccatcagcat gtaactcgag
    36541 agtacgccag ccgggcgcga tagtatccag cgtaaagttg gaacagtgcg gcttaaactg
    36601 cacacaggtc gacggcgttg ccagcaggcg gcgaccgttc cagtcgagat ccagctcctg
    36661 atgaatatga ccgcacagca agtatttgac gtgcggaaac ttcgccagca cggtatccag
    36721 ttcgcccgcg ttacgcagac tgtgttgatc gagccaacta caacccgcag gtagcggatg
    36781 atgatgcagc agcagcaacg tatggcgttc tggcgcatcg gccagtttac gttccagcca
    36841 ctcaagctga aactcgctca gctcaccgtg cggcacgcca aacacctggc tatccagcaa
    36901 caggatttgc cattgctcac caataaacac ccgcttcgcc ggggagatac ccgcatcctg
    36961 taacgcgctg tacatcgcgg gctggaaatc gtggttgccc ggcagccaga cgcagggcgc
    37021 acgaaaactt gcgatgcctt cagcgaaatg ctgataggcc gcagaggatt gatcctgcgc
    37081 taaatcacct gtcgcgacaa tcaggtcgaa ttcgtgctga tgtggacgaa tcgcctccag
    37141 caccgcctgg tagctttccc aggtgtttac ccctaacaga gcttcgtgct tttgtgcaaa
    37201 caggtgagtg tcggtaattt gtaaaatcct gactctggcc tcaccagcca gaggaagggt
    37261 taacaggctt tccaaatggt gtccttaggt ttcacgatgc tagtaaaccg gaatcgccat
    37321 cgctccatgt gctaaacagt atcgcaacca gtccgctaaa aactgattaa tttgatgctt
    37381 ttcgtcgcgt tgatgcaact ttttattagg ataatcatac cgcgctttga agcgaaaaat
    37441 ctgctggctt gaacacactt cagccaccat cgcgtcatga tacagacgca ccgtcattga
    37501 cggaaggctc cagtaactga tcgcgggcgc agtctgttct attgtcacca gggtagtgta
    37561 tcgggtcgat tccacaatcg tcagccgata ttgtgcgttt gccacctgat agcttacagt
    37621 ttcgccgggt gcgtcattgc gcggtaacaa acggcgcaat tgtgaaaagt tcatctcgca
    37681 caggcgcatc atttcaggaa agtcaggtgt gtaacgcttc atttatgccc actcattttt
    37741 taacgcttga tgatgcagct gcagccattg caaagcgatg accgacgctg cgttgtcgat
    37801 tttcccctct tctacccact ggtatgcctg ttcccggctt accacatgaa cgcgaatatc
    37861 ttcgttttca tcagccagac catgaatacc gcttgcggtc gtggcgtcca cttcgcccac
    37921 cataattgac gaacgctcac tggtgccccc cgggcttgcc aggaaactta acaccggttt
    37981 ggtccgtttg actatcagtc ccgcctcttc aatcgcttcg cgacgggcaa catcttccac
    38041 actttcaccc tcttctatca tcccggcaac catctccagt agccaggggg tttcactggt
    38101 gtcgtatgcg gcaatccgaa tctgctcaat cagcacaact tcatcgcgca ctgggtcaaa
    38161 gggtagcaag actgcggcgt gaccgcgctc aaaaatttcc cgccgtacct catgactcat
    38221 ttgcccgttg aatagacgat gacgaaatct ataaagatct aatgaaaaaa agccgcgata
    38281 aagtgtttct cgtgcaataa tttctacatc gtttttgcca aatgtaacgg gcaggttgtc
    38341 tggcttaagc attgttaatg tcctggcact aatagtgaat taaatgtgaa tttcagcgac
    38401 gtttgactgc cgtttgagca gtcatgtgtt aaattgaggc acattaacgc cctatggcac
    38461 gtaacgccaa ccttttgcgg tagcggcttc tgctagaatc cgcaataatt ttacagtttg
    38521 atcgcgctaa atactgcttc accacaagga atgcaaatga agaaattgct ccccattctt
    38581 atcggcctga gcctttctgg gttcagttcg ttgagccagg ccgagaacct aatgcaagtt
    38641 tatcagcaag cacgccttag taacccggaa ttgcgtaagt ctgccgccga tcgtgatgct
    38701 gcctttgaaa aaattaatga agcgcgcagt ccattactgc cacagctagg tttaggtgca
    38761 gattacacct atagcaacgg ctaccgcgac gcgaacggca tcaactctaa cgcgaccagt
    38821 gcgtccctgc agttaactca atccattttt gatatgtcga aatggcgtgc gttaacgctg
    38881 caggaaaaag cagcagggat tcaggacgtc acatatcaga ccgatcagca aaccttgatc
    38941 ctcaacaccg cgaccgctta tttcaacgtg ttgaatgcta ttgacgttct ttcctataca
    39001 caggcacaaa aagaagcgat ctaccgtcaa ttagatcaaa ccacccaacg ttttaacgtg
    39061 ggcctggtag cgatcaccga cgtgcagaac gcccgcgcgc agtacgatac cgtgctggcg
    39121 aacgaagtga ccgcacgtaa taaccttgat aacgcggtag agcagctgcg ccagatcacc
    39181 ggtaactact atccggaact ggcggcgctg aatgtcgaaa actttaaaac cgacaaacca
    39241 cagccggtta acgcgctgct gaaagaagcc gaaaaacgca acctgtcgct gttacaggca
    39301 cgcttgagcc aggacctggc gcgcgagcaa attcgccagg cgcaggatgg tcacttaccg
    39361 acgctggatt taacggcttc taccgggatt tctgacacct cttatagcgg ttcgaaaacc
    39421 cgtggtgccg ctggtaccca gtatgacgac agcaatatgg gccagaacaa agtgggcctg
    39481 agcttctcgc tgccgattta tcagggcgga atggttaact cgcaggtgaa acaggcccag
    39541 tacaactttg ttggtgccag cgagcaactg gaaagcgcgc atcgtagcgt cgtgcaaacc
    39601 gtacgttcct ccttcaacaa cattaatgca tctatcagta gcattaacgc ctacaaacaa
    39661 gccgtagttt ccgctcaaag ctcattagac gcgatggaag cgggctactc ggtcggtacg
    39721 cgtaccattg ttgatgtgtt ggatgcaacc accacgctgt acaacgctaa gcaagagctg
    39781 gcaaatgcgc gttataacta cctgattaac cagttgaata ttaagtcagc tctgggtacg
    39841 ttgaacgagc aggatctgct ggcactgaac aatgcgctga gcaaaccggt ttccactaat
    39901 ccggaaaacg ttgcaccgca aacgccggaa cagaatgcta ttgctgatgg ttatgcgcct
    39961 gatagcccgg caccagtcgt tcagcaaaca tccgcacgca ctaccaccag taacggtcat
    40021 aaccctttcc gtaactgacg acgacgacgg ggcttcggcc ccgtctgaac gtaaggcaac
    40081 gtaaagatac gggttatctg ccgcattctt cccccttctc gcttcaattt cgaccagcca
    40141 tcctctattc tgatgggtat ttaccactgg tcccggaaga caaaaatgaa acggacaaaa
    40201 tccatacgcc acgcatcgtt ccgcaaaaac tggagcgcac gccatctgac accagtcgct
    40261 ctcgcggttg ccactgtttt tatgctggct ggctgtgaaa agagtgatga aacagtgtct
    40321 ctctatcaaa atgctgacga ctgttcagct gcaaacccag gcaaaagcgc cgaatgtacc
    40381 accgcgtaca acaatgcgct gaaagaagcc gaacgtactg cgccgaaata cgccacccgt
    40441 gaagactgtg ttgctgaatt tggtgaaggt cagtgccagc aggcaccagc ccaggctggc
    40501 atggcaccag aaaaccaggc gcaggcccag caatccagcg ggagtttctg gatgccgctg
    40561 atggccggtt acatgatggg gcgtctgatg ggcggcggcg cgggatttgc acagcagccg
    40621 ctgttctcct cgaaaaaccc agccagcccg gcttacggta aatataccga cgcgacgggt
    40681 aaaaactatg gcgcagccca gccaggccgc accatgaccg taccgaagac agccatggca
    40741 ccaaaaccgg cgaccactac taccgttacc cgtggcggtt ttggtgaatc tgttgccaaa
    40801 caaagcacta tgcagcgtag cgcaaccggc acctcttctc gttcaatggg tggctgatac
    40861 cgatggaaag agtcagtatt accgagcgcc cggactggcg cgaaaaagcc cacgaatacg
    40921 gtttcaattt tcacaccatg tacggcgagc cgtactggtg tgaagatgct tactacaagt
    40981 tgaccctcgc ccaggttgaa aagctggaag aagtcaccgc cgaactgcac cagatgtgcc
    41041 tgaaagtggt ggaaaaagtg atcgccagcg atgagctgat gaccaaattc cgcattccaa
    41101 aacgcacctg gagttttgtg cgtcagtcat ggctgactca ccagccatcg ctttattcgc
    41161 gtcttgatct ggcatgggat ggcactggcg aacctaaact gctggaaaat aacgccgata
    41221 caccaacgtc actatacgag gcggcgttct ttcagtggat ctggctggaa gatcagctta
    41281 acgccggtaa cttgccggag ggcagcgacc agtttaacag tctgcaagaa aagctaatcg
    41341 atcgctttgt cgagttgcgt gaacagtatg gcttccagtt gctgcatctc acctgctgtc
    41401 gcgacacggt ggaagatcgc ggaaccattc agtatttgca ggactgcgcg acggaagctg
    41461 aaatcgctac cgagttcctc tacattgacg atatcgggtt aggtgaaaaa ggtcagttca
    41521 cggatttaca ggatcaggta atttccaacc tgttcaagct gtatccatgg gaatttatgt
    41581 tgcgtgagat gttctccacc aagctggagg atgcaggcgt acgctggctg gaaccggcgt
    41641 ggaagagcat tatctccaac aaagcgcttc taccgctact gtgggagatg ttcccgaatc
    41701 acccgaacct gctgcccgct tattttgcgg aagatgatca tccgcaaatg gaaaaatatg
    41761 tggttaaacc gatcttctcc cgtgaaggcg caaacgtgtc gatcattgag aacggcaaaa
    41821 ccattgaagc agcggaaggt ccgtatggcg aagaagggat gattgttcag caattccacc
    41881 cgttaccgaa attcggcgac agctatatgc tgattggtag ctggctggtg aacgatcaac
    41941 ctgccggaat tggcattcgt gaagaccgtg cgttgatcac ccaggatatg tctcggtttt
    42001 atccacatat ttttattgag taatcaatga tgccggatgg cattcgccat ccggtaattg
    42061 ttaacctatc tgcaccgaca gcatactcag gctgcccatt tctataccat cgaccggaat
    42121 ggtaattggc tcctgcccat cccacgcacc taagacatac aacaacggca gatagtgctc
    42181 cggcgttggg ttcgataacg cgccaccttc atggtcaagg taattcacca gaggatgctg
    42241 ctctaccgcc ccttgccacg tcagattcgc tttcacatac tcattaaacg acatcgccca
    42301 aggatatggt gaactatcac cgtgccactt cactgtacgc aggttatgca ccacgttgcc
    42361 actggcgacc aacattattc cttcatctcg cagcgctgcc agtttgcgcc ccatttcgaa
    42421 atgccaggcg gcaggtttgc tactgtcgat actcaactgc accatcggga tatcggcgtc
    42481 aggatacatc ttaatcagca cgccccagga accgtggtca aagccccagg cttctttatc
    42541 cagcgtcacc gggaccgacg ctaacagctc aaccagatgc tgtgccagca caggcgaacc
    42601 cggagcagga taatgcgtat cgtacagcgc ctgcgggaag ccaccaaagt catgaatcgt
    42661 gggcggcgtc tccatcgcgg tcactcctgt tccacgggta aaccagtgag ccgaaaccac
    42721 cacaatcgct tgcgggcgtg gcaatgtcat ccccaacttc tgccagctgc gggtatacaa
    42781 attatcttcc agcacgttca tcggactacc gtgacctaaa aacaatgctg gcatacgtgt
    42841 tgaagacatg atgatatcct taactaaagg tgtcattttg atatcctcac aatacgcttg
    42901 ttcggcggag taagaacccg gataacaatg atgatgatca tcagttattt tgacgatctg
    42961 cctgaaggtg aagatttata aggagttgtc gatgtcagta cctctcattc tgaccatact
    43021 ggcgggggca gccacgttta ttggcgcgtt tctcggcgtt ctcgggcaaa aaccctcgaa
    43081 ccgcttactg gcgttttcgc taggatttgc ggcggggatc atgttgctca tctcattaat
    43141 ggaaatgctt cctgccgcac tagcagctga aggaatgtcg cctgtgttgg gttatggaat
    43201 gtttatcttc ggtctgcttg gctattttgg cctggaccgc atgttgccac atgcgcatcc
    43261 gcaggattta atgcaaaaat cggtgcagcc gttgccaaaa tcgatcaagc gcacagccat
    43321 tctgctcact ctcggcatca gtctgcataa cttcccggaa gggattgcca cctttgtcac
    43381 ggcgagcagc aacctggagc tgggatttgg catcgcactg gccgtcgcgt tgcacaatat
    43441 ccctgaaggt ctggcagtgg caggcccggt ttatgcggca acggggtcta aacgcaccgc
    43501 gattctgtgg gcggggattt ctggactagc agaaattctt ggtggtgtgc tggcgtggtt
    43561 aatcctcggt agcatgattt ccccggtggt catggcggca atcatggcgg cggttgcagg
    43621 aattatggtg gcgctctcgg ttgatgaatt aatgccgctc gccaaagaga ttgaccctaa
    43681 taataacccc agctatggcg tactatgtgg aatgtcagtg atgggattca gtttagtgct
    43741 gctacaaacg gcgggaattg gttaaaaaca acatcagaag ttgtccagca catggacaac
    43801 ttctgccagc ttcacgaaag ccttattcta ccttttcggc tgtctcttct ctcgtactgt
    43861 ttaagtattt gttccgcgct tcaagcagtg cgtctctgtc ctggcgaaac gcccgacaat
    43921 aacgtttcaa atgcaggaaa taacccacca caaccagagc aatcaataca atccagtacc
    43981 aggcgataaa catcctttta cccttaatat ttaacaatac taaacattta gcgcataaat
    44041 ttcacatatc ctttttcgga tatattcata tggtcgcata ataacggaac aattatatga
    44101 ttttgatcat aatgaaaaag aatgcctgat attaatgaaa ttttctcata gaaagagaag
    44161 cggaattaac atcttgcata gcagggcaaa ataaaaaccg aagccagcta caacttcggt
    44221 ttcttgatta aggcagtaat taagcagcgg ttttcagctg gctttacgct catgtgcctg
    44281 acggtatgcc accaggtctt caatagtcac gagcgccata ttgtgtttat tggcaaactc
    44341 aatgcactct ggtgcacgcg ccatcgtgcc atcgtcatta gtcagctcac acagcacacc
    44401 agccggttta aagcctgcca gcgtcatcaa atcaatagtc gcttcagtat gaccgccacg
    44461 cgtcagtaca ccacctgcct gcgcgcgaag tgggaaaacg tggcccgggc gattcagatc
    44521 agaaggtttt gcgccatcgg caatcgctgc gcgaacggtc gtaatacgat cagcggcaga
    44581 aacaccggta gtcacacctt cagccgcttc aatggtcacg gtaaaaccgg tgccataggc
    44641 gctggtgtta ttttctacca tcattggcag atcaagttgt ttacggcgat cttcagtgat
    44701 gcacaggcaa acaataccgc taccgtggcg aatggtcagc gccatctgct caacggtcat
    44761 ggtttctgcc gggaagatca tatcgccttc gttttcacgg tcttcatcat caagcaccat
    44821 tacaccgcgt ccttcacgca gcgcagccag tgcattttca acacgttcga aaggcgtacc
    44881 aaaagaggaa agtagcgtct gattcatggt aaaaaaacct cactaaaatt atggttacca
    44941 gaatcagggc agtcttagga gtggcggcat atagccaaaa taacgtgagc gggtccatgc
    45001 ccgacagaat cgttactctc tcccatccgg actctaaccg tcggccccgg aattacaccg
    45061 gatctgctgt cctttgagtt cgcacccaaa gcgctcgcgg gctttcaact gagttgattt
    45121 accgccggtg gggaatttcg ccccgccctg agaataagcg gattcactat aacgctaatg
    45181 attagcggca gcaacgcata gcttcacata attctggttt atgacttacc cttatcgcac
    45241 tacaatggta ctcaacacct atcagtacag ggaaactaca atgattgacc cgaaaaaaat
    45301 tgagcaaatc gctcgccagg ttcacgaatc aatgcctaaa ggaatcaggg agttcgggga
    45361 agatgtggag aaaaaaatcc gccaaaccct acaagctcag ctgacgcgcc tcgaccttgt
    45421 aagccgtgaa gagttcgacg tccaaacgca agtcctgtta cggactcgtg agaaactggc
    45481 gcggcttgaa caaagaatga gcgaactgga aaatcgcagt actgagatta aaaagcagcc
    45541 agatccagag actctccccc caacgctgta aacatttgtc tttaatgcac tgtaaattat
    45601 gaattaatct tctaatt
//
LOCUS       DAZRUF010000005        44764 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZRUF010000005 DAZRUF010000000
VERSION     DAZRUF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44764
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.005"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(353..895)
                     /gene="ycfP"
                     /locus_tag="ACHXOL_000218"
     CDS             complement(353..895)
                     /gene="ycfP"
                     /locus_tag="ACHXOL_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR6807306.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(918..1943)
                     /gene="nagZ"
                     /locus_tag="ACHXOL_000219"
     CDS             complement(918..1943)
                     /gene="nagZ"
                     /locus_tag="ACHXOL_000219"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR6807307.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1954..2778)
                     /gene="thiK"
                     /locus_tag="ACHXOL_000220"
     CDS             complement(1954..2778)
                     /gene="thiK"
                     /locus_tag="ACHXOL_000220"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR6807308.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2759..3400)
                     /gene="lpoB"
                     /locus_tag="ACHXOL_000221"
     CDS             complement(2759..3400)
                     /gene="lpoB"
                     /locus_tag="ACHXOL_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR6807309.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3414..3791)
                     /locus_tag="ACHXOL_000222"
     CDS             complement(3414..3791)
                     /locus_tag="ACHXOL_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR6807310.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3794..4153)
                     /gene="hinT"
                     /locus_tag="ACHXOL_000223"
     CDS             complement(3794..4153)
                     /gene="hinT"
                     /locus_tag="ACHXOL_000223"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR6807311.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4487..6676
                     /gene="fhuE"
                     /locus_tag="ACHXOL_000224"
     CDS             4487..6676
                     /gene="fhuE"
                     /locus_tag="ACHXOL_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR6807312.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLVFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(6736..8169)
                     /gene="ptsG"
                     /locus_tag="ACHXOL_000225"
     CDS             complement(6736..8169)
                     /gene="ptsG"
                     /locus_tag="ACHXOL_000225"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR6807313.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8464..9261)
                     /locus_tag="ACHXOL_000226"
     CDS             complement(8464..9261)
                     /locus_tag="ACHXOL_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR6807314.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9272..10276)
                     /gene="holB"
                     /locus_tag="ACHXOL_000227"
     CDS             complement(9272..10276)
                     /gene="holB"
                     /locus_tag="ACHXOL_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR6807315.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10273..10914)
                     /gene="tmk"
                     /locus_tag="ACHXOL_000228"
     CDS             complement(10273..10914)
                     /gene="tmk"
                     /locus_tag="ACHXOL_000228"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR6807316.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10904..11926)
                     /gene="yceG"
                     /locus_tag="ACHXOL_000229"
     CDS             complement(10904..11926)
                     /gene="yceG"
                     /locus_tag="ACHXOL_000229"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR6807317.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11929..12738)
                     /gene="pabC"
                     /locus_tag="ACHXOL_000230"
     CDS             complement(11929..12738)
                     /gene="pabC"
                     /locus_tag="ACHXOL_000230"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR6807318.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12858..14099)
                     /gene="fabF"
                     /locus_tag="ACHXOL_000231"
     CDS             complement(12858..14099)
                     /gene="fabF"
                     /locus_tag="ACHXOL_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR6807319.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14187..14423)
                     /gene="acpP"
                     /locus_tag="ACHXOL_000232"
     CDS             complement(14187..14423)
                     /gene="acpP"
                     /locus_tag="ACHXOL_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR6807320.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14634..15368)
                     /gene="fabG"
                     /locus_tag="ACHXOL_000233"
     CDS             complement(14634..15368)
                     /gene="fabG"
                     /locus_tag="ACHXOL_000233"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR6807321.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15381..16310)
                     /gene="fabD"
                     /locus_tag="ACHXOL_000234"
     CDS             complement(15381..16310)
                     /gene="fabD"
                     /locus_tag="ACHXOL_000234"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR6807322.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16326..17279)
                     /gene="fabH"
                     /locus_tag="ACHXOL_000235"
     CDS             complement(16326..17279)
                     /gene="fabH"
                     /locus_tag="ACHXOL_000235"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR6807323.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17347..18417)
                     /gene="plsX"
                     /locus_tag="ACHXOL_000236"
     CDS             complement(17347..18417)
                     /gene="plsX"
                     /locus_tag="ACHXOL_000236"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR6807324.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18498..18671)
                     /gene="rpmF"
                     /locus_tag="ACHXOL_000237"
     CDS             complement(18498..18671)
                     /gene="rpmF"
                     /locus_tag="ACHXOL_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR6807325.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18723..19244)
                     /gene="yceD"
                     /locus_tag="ACHXOL_000238"
     CDS             complement(18723..19244)
                     /gene="yceD"
                     /locus_tag="ACHXOL_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR6807326.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            19443..20027
                     /gene="yceF"
                     /locus_tag="ACHXOL_000239"
     CDS             19443..20027
                     /gene="yceF"
                     /locus_tag="ACHXOL_000239"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR6807327.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENACLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20139..21098)
                     /gene="rluC"
                     /locus_tag="ACHXOL_000240"
     CDS             complement(20139..21098)
                     /gene="rluC"
                     /locus_tag="ACHXOL_000240"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR6807328.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21671..24856
                     /gene="rne"
                     /locus_tag="ACHXOL_000241"
     CDS             21671..24856
                     /gene="rne"
                     /locus_tag="ACHXOL_000241"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR6807329.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25051..26004)
                     /gene="flgL"
                     /locus_tag="ACHXOL_000242"
     CDS             complement(25051..26004)
                     /gene="flgL"
                     /locus_tag="ACHXOL_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR6807330.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(26016..27659)
                     /gene="flgK"
                     /locus_tag="ACHXOL_000243"
     CDS             complement(26016..27659)
                     /gene="flgK"
                     /locus_tag="ACHXOL_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR6807331.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27725..28666)
                     /gene="flgJ"
                     /locus_tag="ACHXOL_000244"
     CDS             complement(27725..28666)
                     /gene="flgJ"
                     /locus_tag="ACHXOL_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR6807332.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28666..29763)
                     /gene="flgI"
                     /locus_tag="ACHXOL_000245"
     CDS             complement(28666..29763)
                     /gene="flgI"
                     /locus_tag="ACHXOL_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR6807333.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29775..30473)
                     /gene="flgH"
                     /locus_tag="ACHXOL_000246"
     CDS             complement(29775..30473)
                     /gene="flgH"
                     /locus_tag="ACHXOL_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR6807334.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30526..31298)
                     /gene="flgG"
                     /locus_tag="ACHXOL_000247"
                     /pseudo
     CDS             complement(30526..31298)
                     /gene="flgG"
                     /locus_tag="ACHXOL_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(31470..32225)
                     /gene="flgF"
                     /locus_tag="ACHXOL_000248"
     CDS             complement(31470..32225)
                     /gene="flgF"
                     /locus_tag="ACHXOL_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR6807335.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32245..33450)
                     /gene="flgE"
                     /locus_tag="ACHXOL_000249"
     CDS             complement(32245..33450)
                     /gene="flgE"
                     /locus_tag="ACHXOL_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR6807336.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33475..34170)
                     /gene="flgD"
                     /locus_tag="ACHXOL_000250"
     CDS             complement(33475..34170)
                     /gene="flgD"
                     /locus_tag="ACHXOL_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR6807337.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34182..34586)
                     /gene="flgC"
                     /locus_tag="ACHXOL_000251"
     CDS             complement(34182..34586)
                     /gene="flgC"
                     /locus_tag="ACHXOL_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR6807338.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNMVKSMMLKTLTLGQ"
     gene            complement(34590..35006)
                     /gene="flgB"
                     /locus_tag="ACHXOL_000252"
     CDS             complement(34590..35006)
                     /gene="flgB"
                     /locus_tag="ACHXOL_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR6807339.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35161..35820
                     /gene="flgA"
                     /locus_tag="ACHXOL_000253"
     CDS             35161..35820
                     /gene="flgA"
                     /locus_tag="ACHXOL_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR6807340.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGNGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            35896..36189
                     /gene="flgM"
                     /locus_tag="ACHXOL_000254"
     CDS             35896..36189
                     /gene="flgM"
                     /locus_tag="ACHXOL_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR6807341.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36194..36610
                     /gene="flgN"
                     /locus_tag="ACHXOL_000255"
     CDS             36194..36610
                     /gene="flgN"
                     /locus_tag="ACHXOL_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR6807342.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36650..38185)
                     /gene="murJ"
                     /locus_tag="ACHXOL_000256"
     CDS             complement(36650..38185)
                     /gene="murJ"
                     /locus_tag="ACHXOL_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR6807343.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38295..39218)
                     /locus_tag="ACHXOL_000257"
     CDS             complement(38295..39218)
                     /locus_tag="ACHXOL_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR6807344.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39220..39867)
                     /locus_tag="ACHXOL_000258"
     CDS             complement(39220..39867)
                     /locus_tag="ACHXOL_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR6807345.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39878..40462)
                     /gene="rimJ"
                     /locus_tag="ACHXOL_000259"
     CDS             complement(39878..40462)
                     /gene="rimJ"
                     /locus_tag="ACHXOL_000259"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR6807346.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40698..41906
                     /gene="mdtH"
                     /locus_tag="ACHXOL_000260"
     CDS             40698..41906
                     /gene="mdtH"
                     /locus_tag="ACHXOL_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR6807347.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            41970..42617
                     /gene="grxB"
                     /locus_tag="ACHXOL_000261"
     CDS             41970..42617
                     /gene="grxB"
                     /locus_tag="ACHXOL_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR6807348.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42751..43311
                     /locus_tag="ACHXOL_000262"
     CDS             42751..43311
                     /locus_tag="ACHXOL_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR6807349.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43417..44463
                     /gene="pyrC"
                     /locus_tag="ACHXOL_000263"
     CDS             43417..44463
                     /gene="pyrC"
                     /locus_tag="ACHXOL_000263"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR6807350.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLITCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44537..>44764
                     /gene="dinI"
                     /locus_tag="ACHXOL_000264"
     CDS             44537..>44764
                     /gene="dinI"
                     /locus_tag="ACHXOL_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR6807351.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADD"
BASE COUNT        10839 a        12105 c        11146 g        10674 t
ORIGIN      
        1 cttaattaac aacaggttac agcaatgaaa atgttcttcg ctattgagaa taacatgaat
       61 ggtgcattgg tcataccaaa attgatgtta atcaagtttt gttgctgaaa agataggcga
      121 ctttaccggg tgagatcaaa aaacagagtt tattgtttat tatttgagca agtccggaag
      181 ggtgatatag ggtgacgcgt atatcgaggg cgtttggtcg tcctgataag atgcgcagca
      241 tcgcatcagg cattgatgct cgactgtcgg atgcggcgta aacgccttat ccgacttaca
      301 gaattcggag cgcatcaggt accggcaagg ttacctgatg cgacgagggc atttacccga
      361 gggttttgaa cgccttaatg cgctgcaaat gcggggagat attcttgaat ttgtgcgtct
      421 gctcttcgtc ccagacaatc tcgtaataat gatgcaactc ttcagatgtc cgctggctgt
      481 taagcgcttc atcattacgc gacaaaatca ccaggcaacg atcgcgattc ttctcgcgga
      541 agttggtcac acacttagtg gcaatatcgg catactcttc cgggcgatca atcttgcctt
      601 ccatgttctc ataagggaac aaattagggt tgaagatcac ctggcggata tcgcaaagaa
      661 aaccaatccg ttccgcccag tatccgccca gaccaacgcc gcaaatcagc ggacgctcgt
      721 cgacgttcag ttgcaacatt ttgtccactt ctttgagcag atgctgcata tcatgtttcg
      781 gatgccgcgt actgtagctt atcaagcgta catccgggtc aataaactgc aattgtaaga
      841 ctttctcgtg gttacccgga ctgttagagt caaaaccgtg taaatagata atcatcgctt
      901 cctcacataa gccagggtta gtgacctgct ttctcttcct gccagcgttc atgtaactga
      961 ttcagacggg tgctgatcgc cttccagcga gccgagtcca tcagttcctg tcgcgaaaat
     1021 gaacctttat gatacaaacg tgtaacacgt tctgccttga tcggtgacag attatctaac
     1081 acgctgacgg cccctttacg attattgcag accaggatca tatcgcaacc tgcgtccagt
     1141 gatgcctgcc cgcgttcggc ataactgccc ataatcgcgg caccttccat cgataaatcg
     1201 tcagagaaaa ttacaccgtc aaaacacagt tcctgacgca aaacggtttt cagccagtag
     1261 gaagaaccgc tcgccggacg cggatcaaca tcactgtaga tcacatgcgc aggcataatg
     1321 gcgtcgagtt tattttcgcg gattaaggaa ctgaagaccg acatatcttt agcgcgaatc
     1381 tccgcttgtg gacgtggatc gcacggtgtt tctttgtgtg agtctgccgt tactgcaccg
     1441 tgtcctggga agtgtttccc ggtcgttttc attccggctt catgcatacc atcaataaac
     1501 cggctggcaa ttgccagggc tttttgtgga tcggcatgat aagaacgctc gccaatcgcc
     1561 gcgctgatat gcccgacatc cagcactggc gcaaagctga tatcaatatc catagcgatc
     1621 atttcgctgg ccatcagcca acccgcctct tgcgccagtt tgccgccctc ttccattccc
     1681 aacagcgcag caaaggattg tgctgccggt aagcgggtaa aaccttcgcg aaaacgctgc
     1741 acgcgtccac cttcctgatc taccgccacc accagatgat tgcgcgatgc tgcgcggatc
     1801 tggcgcacca gttcacgtaa ctgggcagga tcatgatagt tacgcgtaaa gagaatcagc
     1861 cctcccacca gcggatgcgc cagtatttca cgctcttccg cgtccagttc gtaaccttcg
     1921 acatccaaca ttactggacc cacactgctc tccttattgt tttattaata gctgccgcca
     1981 ggtgtcatcg gccagcctga taaattgttg atcgccgatt tgtcgccagc ggtactcaaa
     2041 ccaccctgct ttgagcatca gcagccaggg aaaccatcgc ctgacctgac gccataattg
     2101 cgccggataa atcttcgcgc gagtggcata gtcattgacc aattgccggt gctgttcagt
     2161 attttccacc cacaccgccg ccagttccag cgcgatatca ccatctccgg catactccca
     2221 gtcgatgagt tttaaccctg acgcgctatg cactaaattt ccggcgtgga catccatatg
     2281 caatggactt aagcgtaaag gccgtggttc ccgcgctttg cgcagacgtt ttaacattcg
     2341 cagccaaccc acagtccgcc gcgccggatc gctttgctgc cagtacagtt ccagtaaggg
     2401 caacagcgtt attcgccagc caaaacgtgg ttgttgatga agataataca gcaagcctgc
     2461 cagttcgttg gtatccggca aatacgtttt taccgcgccg ggcagatagt cgactaccat
     2521 ccagtcacgg agatataaat gcggcttcgg tgcaatgctt gcgggtagtt gtgataaagc
     2581 ccgatactgg cgcaagaacg cggactgcgg cgcatcagga tcgtgcggct gacgcacaac
     2641 aaaacgctgg ccctgatgtt caatgagaaa actaccgcca ctaagcccac tattaaacgt
     2701 cgtgacggga tgaaactgcg ggaaaaagcg cgacagcaat tcgtcgcgcg tgatgggatt
     2761 attgctgcga aacggcacct ttacctgacc agataatttc gcccgtctgc accagcatca
     2821 gctgcatttg tagggtcgga gcgttaacgt tgccagaggc gctggagtac agcacgtaat
     2881 gagcgccgac attgcgggca atgcctatgg ctttactacg ggtacctaaa ctgtcctgcg
     2941 gcgacaaacc taactgttgc ttagccatcg acagctgctg ggcggaaacc agggtaaatt
     3001 tcccgttatt agccagcgca tttcgcagcg tttcggtcgc ttctgcggca ttcagcgaac
     3061 cgttagtacg gttgttaacg ctatcaacca gcaggacgct acccgcagtc accccgtcag
     3121 ccccaagcat cttactgacc atcggctgca ttgcgccatt ccagtcataa tggcgaatat
     3181 gcggcgcagg cggtgccgtt tgatcttcgt gctcaattgg gcctggctgc tgcgggatcg
     3241 tcggcaccga gggcactgtg gggacaggct gttgtggctc ggctggttgt tccggcgctg
     3301 gtttcacttc ttctaccggt gcaggttcac gttgccccac acacccggcg agaaacatcg
     3361 ccagcgcggt aatcaaggcg tagcgactca tttttgtcat caagattcac cccttacaaa
     3421 tatagataaa gtctgacttt gtgcgccccc agaaaattgg cgctgccgta cagcgttacc
     3481 gccgaatgtg cgggaatgct aacgctgcgt ggcctttcca gaggatgcat ctccagccct
     3541 ctggcgtcat accagtaaaa acgataatga acggtaacgg gttcttgcct ttcgttataa
     3601 agcgttgagg atgctgaagg ttgaatatca gacgtcgaaa ggacgggctt ttctgcactg
     3661 atgcccgcag ccaataaaga tgactccatc accagcgatt gctcatcatt caccggaatt
     3721 tccggatgtg aacgacagcc caccagcagt aacaacgcca gagacaccag cccaaagcat
     3781 ccttttctca tcgttacaga cctttatgcg ccagcattgg tcccagcgga cggccaccca
     3841 acaagtgcat atggatgtgg taaacctctt gtccgccatg gcggttggta ttcatgatca
     3901 gacgatagcc atcttcggca ataccttctt gctcagcaat ttttgccgct acggtgatca
     3961 tgcgtcccag cgcctgctca tgctcagctg acacgtcgtt cacagtcgga atgaggatat
     4021 tcggaatgat cagaatatgc gttggcgctt gcggcgaaat atcgcgaaac gccgttacca
     4081 gatcatcctg gtagacgata tcggagggga tctcacgacg aataattttg ctgaatatag
     4141 tttcttctgc cacgacattt tcctttttca aatagccatg cgttgcgcca tgctacgccg
     4201 cgccacactg cgagtataga gtatggtcga gttaaacctg ctctttcaac cttaactcac
     4261 gatttcttaa gcaaaaaatc gttttgctgc cgtatatatc gccattattc ccatttctgc
     4321 ctgcgaatgc gctgaaaaga cattctctgt ttggtttaca cgacaaatat gaatgcgcat
     4381 atttctcatt tgcatttaca aacaaaatta ttcgcacact aaatataacc gctcatcacc
     4441 ctcctccggc atgagcctga cgacaacata aaccaagaga tttcaaatgc tttcaacaca
     4501 atttaacagg gataatcaat atcaagccat caccaaaccg tcactacttg ccggttgcat
     4561 agcactggca ctattacctt ctgccgcttt tgctgcacca gccactgaag aaacggtgat
     4621 tgttgagggt tcagccacag ctccagatga tggcgaaaat gattacagcg taacgtctac
     4681 ctctgcgggt accaaaatgc agatgactca acttgatatt cctcagtcgg tcactattgt
     4741 tagccagcag cggatggaag atcagcagtt acaaacgctg ggcgaagtga tggaaaacac
     4801 gctggggatc agcaaaagtc aggcggattc cgatcgtgct ctttattatt cccgtggatt
     4861 ccagatcgat aactatatgg ttgatggtat ccccacctat tttgaatcgc gctggaatct
     4921 gggcgacgca ctttctgata tggcactctt tgaacgcgta gaagtagtgc gtggcgcgac
     4981 aggactcatg accgggacgg gtaatccatc tgcggcaatt aatatggttc gaaaacacgc
     5041 gaccagtcgt gaatttaaag gcgatgtctc ggcggaatac ggtagctgga acaaagaacg
     5101 gtatgtggcg gatttacaaa gcccactcac cgaagacggt aaaatccgcg cgcgaattgt
     5161 cggcggctac cagaataacg actcatggct ggaccgctac aacagtgaaa agaccttctt
     5221 ctcgggcatt gtcgatgctg atttaggcga tcttacgacg ctttcagccg gttacgaata
     5281 tcagcgcatt gatgttaata gcccaacctg gggcggttta ccgcgctgga atactgatgg
     5341 cagcagcaac agttacgatc gtgcacgcag taccgcacct gactgggcgt acaacgataa
     5401 agagatcaac aaggtcttta tgaccctgaa gcagcggttt gctgatacct ggcaagcgac
     5461 actgaatgcc acccactctg aagtcgaatt tgacagcaaa atgatgtatg tcgatgccta
     5521 tgtaaacaaa gcggatggta tgctggttgg gccatacagt aattatggac ctggctttga
     5581 ttatgtcggc ggcaccggtt ggaacagtgg caaacgtaaa gttgatgcgc tggatttgtt
     5641 cgctgacggt agttatgaat tgtttggtcg tcagcacaat ctagtgtttg gtggcagtta
     5701 cagcaaacaa aacaatcgtt acttcagttc atgggccaac atcttcccgg atgaaattgg
     5761 cagtttctac aactttaatg gcaatttccc acaaaccgac tggtcaccac agagcctggc
     5821 acaggacgat accacacata tgaaatcgtt atatgctgct actcgagtca cccttgccga
     5881 tccgctgcat ctgatcctcg gcgcacgtta taccaactgg cgggttgata cgctgactta
     5941 cagcatggag aaaaaccaca ccacgcctta cgctggtctg gtgtttgaca tcaatgacaa
     6001 ctggtcgacc tacgccagct atacctctat tttccagccg caaaatgatc gtgacagttc
     6061 aggcaaatat ctggctccaa tcaccggtaa caactacgag ctgggtctga aatcggactg
     6121 gatgaatagc cgtctgacca ccacgttagc catcttccgt attgagcagg ataatgtcgc
     6181 tcagtccacc ggtacaccta tccccggcag caacggcgaa accgcctata aagcggtgga
     6241 tgggacagtc agtaaagggg tggaatttga actcaacggc gcaattaccg acaactggca
     6301 gctgacattt ggcgcaacgc gctatattgc agaggataac gaaggaaacg ccgttaatcc
     6361 taatctgcca cgcaccacgg ttaaaatgtt caccagctat cggttgcctg tcatgccaga
     6421 gttgacagtc ggcggtggtg ttaactggca aaatcgcgtg tataccgaca ccgtgacacc
     6481 gtatggcacc ttccgcgccg agcaaggtag ctacgcgctg gtggatctct tcacccgcta
     6541 ccaggtgacg aaaaacttct cgttacaggg gaacgtcaat aacctgttcg acaaaaccta
     6601 cgataccaac atggaaggtt ctatcgtcta cggcgcaccg cgtaatttca gcattaccgg
     6661 cacgtatcaa ttctgaataa cacctgtaaa aaaggcagcc atctggcggc cttagtctcc
     6721 ccaacgtctt acggattagt ggttacggat gtactcatcc atctcggttt tcaggttatc
     6781 ggatttagta ccgaaaatcg cctgaacacc agaaccagca accactacgc ccgctgcgcc
     6841 cagtttcttc aggccagcct gatccacttt agacacatca gcaacgctga cgcgcagacg
     6901 ggtaatacat gcgtcgaggt tagtaatgtt ttctttacca ccaaatgcag caaccagagc
     6961 cggtgccatt tcgctggtac ctgttgcttt tgcatcttca gtcgcgtctt cacgacccgg
     7021 cgttttcaga tccagtgctt taatcagcac gcggaagatg gtgtagtaaa caatcgcata
     7081 accgataccg acgatcggga acagccacag tttgctgctg ttaccagaca gaacgataaa
     7141 gtcgatcaga ccgtgcgaga acgacgtacc gtcacgcatc cccagaagaa tacagattgg
     7201 gaatgccagg cctgccagaa tcgcgtggat gatgtacagg atcggcgcaa cgaacatgaa
     7261 ggagaactcg atcggctcgg tgataccggt caggaacgag gtcagcgccg cggagatcat
     7321 aataccgccc actttcgcgc ggttttctgg tttagcagag tgccagatag caattgcggc
     7381 agctggcaga ccgtacattt tgaacaggaa gccaccagac agtttacccg cagtcgggtc
     7441 acccgccata taacgcggaa tgtcgccgtg gaaaacctga cctgctgcgt tggtgtattc
     7501 accaatctgc atctggaaag gtacgttcca gatgtggtgc agaccaaacg gcaccaggca
     7561 acgttcgatg aaaccgtaaa tgccaaacgc aactaccggg ttctggtaag cagcccactg
     7621 agagaaggtc tggattgcag aaccaatcgg cggccaaatg aaggacagca caacgccagt
     7681 aaagatggca gccaggccag aaatgatcgg cacaaagcgt ttaccggcaa agaagccaag
     7741 atactcaggc agcttaatac ggtagaaacg gttaaacatg tacgctgcga tcgcaccgga
     7801 gataatccct ccgagtacgc cagtatccgc caggtgttta gaggcgattt cttcagcagg
     7861 taaatgcagt accagtggcg caaccacggc catggtttta accatgatgc cataggcaac
     7921 aactgcggcc agcgcggata cgccatcgtt attggtaaag ccgagggcga caccgatcgc
     7981 aaaaatcagt ggcatgtttg caaagacgga accgcctgct tctgccataa catgcgatac
     8041 aacggcgggc agccagctga aattcgcgga accgacgccc agcagaatac ctgcgatagg
     8101 cagtacggat accggcagca tcagcgattt accgaccttt tgcaggttag caaatgcatt
     8161 cttaaacata attgagagtg ctcctgagta tgggtgcttt ttttacgttc tcacgcgtgg
     8221 caagggggga gagcctcgcc gtgtacaggg catctaagcg ccctttattt attacacaga
     8281 gtaaaataat tcagtgccga tttgtttgac ggctatcacg tttcaacttc agatcattgg
     8341 agaagttgca cattttgaca aaacatatgt cacaatattt caaacgtggt tattcaccgc
     8401 ccaattttcg gcggtgaact ttactcgttt tagccattaa ttacgagctt taaaaaaact
     8461 cattcaacgg atggattgaa ggcgggaagc gtcgatgtga aacagacggg cgaagttatc
     8521 ggtggttacc tgcgccagtt cttcaacggc aacacctttc aacacagcca tgtattctgc
     8581 aacgtcacga accatcgcag gttgattctc ttttcctcga tgcggtaccg gcgcaaggta
     8641 aggtgagtca gtttccacca gtaaccgatc cagggggaca taacgcgcag catctcgaag
     8701 ttgctccgca ttacggaagg tcacaatgcc ggaaaaggag atgtaaaatc cgagatccag
     8761 taatttaccc gccgtttctc tgtcctctgt aaaacagtgt agtacgccac cgcaatccgt
     8821 cactttttct tcgcgcagaa ttgccagcgt atcggcgcgg gcgtcacggg tatggacgat
     8881 aaccggcttg ttcagttcac gaccaatctg gatatgatgg atgaaggact cttgctgacg
     8941 aactttagtt tccggtgtgt aataataatc cagcccggtt tcacccagcg ctacaacacc
     9001 ctcttctgcc gccagacggc gtaaatcttc tacatcgtag ggatcattct ggttgagtgg
     9061 atgcacgcca caagaaaata cgacgttgtc acgttcgcct accagatccc gcatatgtaa
     9121 ataacccggt aatgttgtgg cgactgccag acaaaatttc acatcgcgtg cggcggcttt
     9181 cgccagaacg tcatccacgt ccttatgcaa agattcataa tccagaccat cgagatggca
     9241 gtgtgagtcg actaaaaaca tgatgtctct cttaaagatg aggaaccggt agcacaacgc
     9301 ccggttgcag gtaatgctca atacgcagta aaagatcggt gatgagaagc tcgcggttga
     9361 tgcctgtgac agacattaac tgttcacgaa tgtggcaaac atcccccagt atagcctgca
     9421 ggcgcgaggg agaaagatgg tttgccagtt cgacgacaag gcccggcaca tcaacattgg
     9481 tcacctgcgc agcaccatga tggcgtttta gcgcatccat cagcaacgtt gccagccagt
     9541 gtaaacgcgc cggagcttgt tcatgattaa gggccgccag cagcgaatac cagtcgcccg
     9601 atggcacgct atatgccaat gcctgacaca atgtttcacg agcctgccag ttatctccct
     9661 gaaacaacgc cagtgccgcg ccaggcgaac cggcgcttaa gcgcaatgcg gcaagtaatg
     9721 catcctgtga cattgtcact tcgcgtgaaa gccaggtcac ggcgtactgt tccggcggcg
     9781 gcgcaaggta atgtaaccga caacgactac gtaatgttgc cagtaaacgt tcaggctcgc
     9841 gggtagccag gaaaaaccag gtttctgctg gtggctcttc aagcgttttc agcaatgcgt
     9901 tagccgcggc gtcggtcagt aaggcagcat cggttaccca gacaactttc gcaccaccta
     9961 ggcgtgcgtg ctcattcagc ttttcggtga cctcacgtac tgcatcaatg cccagcgtat
    10021 ttttcccttt ttcgggagcc agggtgtagt aatcgggatg cgtgccagcc tgcatcaact
    10081 gacatccacg acagtgaccg caacttttgt ggccctgcgg ttgttggcag agtaaataac
    10141 ggctcagggc gtagattaaa gcatcatcgc ccatgcccgg taacgcctga atgagtagcg
    10201 catggtgacc tcttccggcc tgatagctgg ctaccagttt ttcgaaatca ggtcgtaacc
    10261 atggatacca tctcatgcgt ccaactcctt cacccagtgg gtcacggtag tgcggattgc
    10321 atccatcacg gcctccagcg gctgggtggc atcaatggta tgaatacttt tatcttgtgc
    10381 agccagttcc agatagcggg cgcgggtgcg attaaagaaa tcgaaagact cttgctcaat
    10441 acgatccagc tcgccgcgcg cacgcgcgcg ttttaagcca acttccgggg taacatcgag
    10501 atagagcgtt aagtcggggc gaaaatcccc gagaacagca tcacgcagtg ttgccagcat
    10561 atgttggtca ataccacgtc ctccgccctg atacgcctga gtggagagat cgtggcgatc
    10621 gccaatcacc caggtgccgt tagccagagc tggtttgatg accgtttcta ccagttgaac
    10681 gcgcgcggca taaaacatca gaacttcggc tttatcggta atgacttcat cgcctaccga
    10741 tttgatatcc agcaccaggc ttcttaactt ttcggcaagt tgcgtaccgc caggttcccg
    10801 agtgaaaacc atgtcgcgga tacccagttg ctcaagcgtc tcaaccacca cattacgcgc
    10861 ggtagttttg cctgcgcctt ccagcccctc aatgacgata tacttactgc gcattttttt
    10921 ccttaagcac tttcagataa tcctgcacag acttgttatg gctggcaaga ttggtattaa
    10981 acgtgtgacc acctttacca tcggccacaa aatagagata cggcgttttt gccggatgcg
    11041 cagcagcctt cagcgaatcc gcccccggcg tagctatcgc acccggcggc aaaccggtaa
    11101 tggtataggt gttatacgct gtcggcgttt ccaggtctgc acgagaaagt ttgccattat
    11161 aacgctctcc catcccgtaa atcacggttg ggtcggtctg caggcgcata ccaatgcgta
    11221 aacggttgat aaataccgag gcaacctgat cgcgttcact ggcaacggcg gtttcttttt
    11281 cgataattga tgccatcgtc accagctgat ttttatcttt ataaggcaga ccgtccgcac
    11341 gcccttccca ggcgctatcg accgctttca ccattttctt gtgcgctcgc ttgagtaacg
    11401 cgacatcggt ggtattggcg gtatacatcc aggtgtctgg ccagaaccaa ccttcaatcc
    11461 actccgggtt ttccagttca agtgcctgcg ctacggtggc gtacttatcg tcactcagcg
    11521 tgtgcttgat atacggagcc tcacgcaatt gcttgaggta gtcgctcaga cgcatccctt
    11581 ctaccagtcg cagcgggaac tgtgcttctt taccgctttc cagcaatttc agcatctcgc
    11641 gcacggtcat ctgcggtgta aagcggtaag tcccggcttt aaagtgagaa agatccggtt
    11701 cgatacgcag caaccattga aaaacccgtg ggcgattgat gatcttatcg gcataaagct
    11761 gttcaccaag cgccagacgt ccggtccctg gcttcagggt aaatatcgtc tcttctttga
    11821 taagcaattt gctgtcggca agatggcgaa ccttccagac gcccacacca gcggcgatac
    11881 ccagtaccac cagcaataac aagataatca ataacacttt tttcatgact aattcgggcg
    11941 ctcacaaagt ggggctaaat attcatataa cgttgctgac gaaaaggaga catcgccaca
    12001 ggcacgtacg ggcatcactg gcattaacgc attacaaata accatctcat ctgcctgcaa
    12061 cgcctcttcc agagaggctt gcacttcgac aagctgataa gaggattgtg ccagcaaacg
    12121 gatacagaat tgtcgcataa tgccgttaac acctgcctga tccagacgcg gcgtataaac
    12181 tacattgccc ttccgccaga acaaattagc cgcacagcat tccgtaaccc acccttcgct
    12241 gtcaaggacc agcgcctcat cagcgtttgt ctgctcaaga tgagagcgga tcaatacttg
    12301 ctcaagccga ttaagatgtt taatacctgc aagatgagga ttgcgcccca gccgcaccgg
    12361 gcttagcgcc aacgtcatcc cttcgttacg caaacggtcg taatgtgcag gataaaccgt
    12421 aacggagaga atccgcgttg ccggtccact gttcaatgtg ctgtaccctc gcccgccact
    12481 accgcgactg atcacgactt tcagcacacc attttgctgt tctgctgcca gcgttttcat
    12541 ctcttgttca agctgaggcc agaagtcaca gaaaatcatc aaccgctgac aagcatcctg
    12601 tagtcgctgg atatgcgccg ataacaaact gactttaccg tcgataactc tggcggtggt
    12661 aaaacaacca tcaccaaact gcgttgcccg atcgcttact gccagcgatt cctgcttatg
    12721 accgttaatt aagaacatac cggctcctta tcagagaatt gttagtgtgg cagcatgttc
    12781 actacggaac aagtcggaat aaaagctaag aaaaaaggcc cgcaagcgga ccttttatat
    12841 gggtgggaaa tgacaactta gatcttttta aagatcaaag aaccattagt gccaccgaag
    12901 ccgaaggagt tacacagcgt gtattccatt ccgctgacct gacgtgcttc atgcggtacg
    12961 aaatccagat cgcaaccttc atccgggtta tccaggttga tggttggcgg aacagcctga
    13021 tcgcgcagcg ccaggatgga gtagatagat tctactgcac ccgccgcacc taacaggtga
    13081 ccggtcatag atttcgtgga gcttaccaac acacggcttg cagcttcacc gaagatggtt
    13141 ttcaccgcct gcgcttcagc tttatcgcca gccggcgtag aagtaccgtg cgcgttaacg
    13201 tagccaatct gactcgcttc aatgcctgca tcacgcagag catttgccat cgccagagct
    13261 gcgcctgcgc cattttctgg cggtgacgtc atatgataag catcgctgct cataccaaag
    13321 ccgacgagtt cagcgtaaat tttcgcaccg cgttttttcg cgtgttcgta ctcttcaagt
    13381 accagcatac cggcaccatc gcccagtacg aagccatcac gctctttatc ccacgggcgg
    13441 ctcgccgctt gcgggttatc attgcgggta gataatgcac gtgccgcgcc aaaaccacca
    13501 acacccagcg gcgtactggc tttctctgcg ccacctgcaa ccatcacgtc agcatcgcca
    13561 tacgcgataa tacgcgcagc atggccaatg ttgtgcacgc cggaagtaca ggcggtcgcg
    13621 atagagatgc ttgggccacg caggccatac atgatagtca gatgacctgc caccatgttc
    13681 acaatcgttg acggaacgaa gaatgggctg atcttacgtg gaccaccgtt catcagagat
    13741 gtgtggtttt cttcgatcag tccgaggccg ccaatcccgg agccaattgc ggcaccaatg
    13801 cgggttgcgt tctcttccgt tatatcaagg ccagaatcct gcatggcctg aacgccagcg
    13861 acaattccat attgaatgaa ggcatccatc ttgcgctgtt ctttgcgcga gataatgtcc
    13921 tcacagttaa aatcctttac taagccagca aatttcgttg cataggcgct agtatcgaaa
    13981 tggtcgatta ggctgatgcc actctgaccg gcaagcagag ctttccaggt agactctacg
    14041 gtattgccga caggagacaa catgcccagt ccggtcacaa ctacacgacg cttagacacg
    14101 tttgtcctcc agggagggaa aaaatgattc tagtgggaca aaaagataaa actcaggcgg
    14161 tcgaacgacc gcctggagat gttcacttac gcctggtggc cgttgatgta atcaatggca
    14221 gcctgaacgg tggtgatttt ctcagcttct tcgtccggaa tctcagtatc aaactcttct
    14281 tccagagcca ttaccagctc aacggtgtca agagaatccg cgcccaggtc ttcaacgaaa
    14341 gaagcattgt tggtaacttc ttcctgctta acgcccagct gttcgccgat aattttctta
    14401 acgcgttctt cgatagtgct catactctta aatttcctat caaaactcgc tttcgcgatg
    14461 gttttcgtag tgtataaaat gttgaaaaat ttgcaactaa atcccggcag gtcttaccac
    14521 gattttacgt tattttgagg ccttaccccc caataacgca aatcattttg cactaattat
    14581 tactcataac cacgcagact gcgcgcaaca tgagcatttt gtgcaaatcg cggtcagacc
    14641 atgtacatcc cgccgttcac atgcaaagtt tcacccgtga tgtaagctgc ttcgtcggat
    14701 gccaggaatg caaccgcgtt ggcgatttcc tgtgcgccgc cgaggcgacc cgcaggaacc
    14761 tgcgccagga tacccgcacg ctggtcatcg ctcagcgcac gtgtcatgtc cgtttcaata
    14821 aagcccggag caacaacgtt tacagtaata ccgcgtgacg caacttcgcg cgccagtgat
    14881 ttactgaagc cgatcaagcc cgctttcgcc gcagcgtagt tggcctgacc gccatttccc
    14941 atggtaccaa ccacagaacc gatagtgata atacgaccat gacgcttttt catcatagcg
    15001 cgcattaccg cttttgacag acggaaaaca gatgaaaggt tggtttcgat aatatcgttc
    15061 cactcttcat ctttcattcg cattaacagg ttatcacgag tgataccggc attattgacc
    15121 aggatatcca cttcaccaaa ttctgcgcga attttttcca gaacagattc gatagatgcc
    15181 gggtcggtca cattcaacat cagacctttg ccgttggcac ctaaataatc gctgatcgcc
    15241 tgagcgccat tttcactggt cgcagtgcca ataactttcg cgccacgggc tgcgagcgtt
    15301 tcagcaattg cgcggccaat tccgcggctt gcaccggtta ccagtgcgat ttttccttca
    15361 aaattcatga ttttcctctt ttaaagctca agcgccgctg ccatcgctga aggttcgttc
    15421 agcgccgagg cggtcagggt gtcgacaatg cgtttcgtca ggccagtaag caccttgccc
    15481 gggccgactt catagagatg ttctacgcct tgcgctgcca tgtactcaac agacttcgtc
    15541 cactgaaccg ggttatacaa ctgacgtacc agtgcgtcac ggatggcatc accattggtt
    15601 tcgcatttca catcaacgtt attcacaaca gaaactgttg gtgcgttaaa ggtgattttc
    15661 gctaattcta ctgccagttt gtcggctgct ggtttcatca gcgcacagtg agacggtacg
    15721 ctcactggta acggcagagc acgttttgcg cccgcagctt tacaggcagc gccagcacgc
    15781 tcaaccgctt ctttatgacc ggcaataacc acctgtcccg gagagttaaa gtttaccgga
    15841 gaaacgacct gacctgctgc agcttcttca cacgctttcg caatagacgc atcatccaga
    15901 ccgatgattg ccgccatagc gcccgtacct tccggtacgg cttcttgcat gagcttgccg
    15961 cgcatctcaa ccagacgcac cgcatcagcg aaatcaatca caccagcgca aaccagcgcg
    16021 gaatattccc ccaggctgtg accggccatc attgccggtg ctttaccgcc ctgctgctgc
    16081 catacgcggt acagcgcaac agaggcagtc aacagcgctg gctgggtttg ccaggtttta
    16141 ttcagttctt cagctggccc ctgctgggtc agcgcccaca ggtcgtagcc cagcgccgca
    16201 gaagcttcag caaacgtttc ttcgacaatt ggatagctcg ccgccatatc agccagcatt
    16261 ccaacggttt gagaaccctg tccagggaac acaaatgcaa attgcgtcat gttttaatcc
    16321 ttatcctaga aacgaaccag cgcggagccc caggtgaatc caccgccaaa ggcttcaagc
    16381 agaaccaact gccccggctt aatgcgcccg tcgcgtacag cttcatccag cgcgcacggg
    16441 acagaggccg cagaggtatt accgtggcga tccagcgtca ccacgacatt gtccatagac
    16501 ataccgagtt ttttcgccgt tgcactgata atacgcaggt tagcctgatg cggaaccagc
    16561 cagtccagtt gagaacggtc aagattattt gccgccagcg tctcatcaac gatgtgcgcc
    16621 agttccgtta ccgcaacctt gaagacttcg ttgcccgcca tcgtcagatg aattgaattc
    16681 tctggattca cacggtcagc attaggcagc gtcagcaact caccatagct accgtcggca
    16741 tgcagatggg tggagatgat tcccggctct tcagaggcag ccagcaccgc agcgcccgcg
    16801 ccatcgccaa aaataataat agtcccacga tcggttggat cgcaggtgcg cgccagtaca
    16861 tcggaaccga cgaccagagc atacttcacc gccccagatt tcacgtattg atcggctacg
    16921 cttaatgcat aggtgaaacc tgcgcaggct gctgcaacgt caaatgccgg gcaaccttta
    16981 atgcccagca tgctttgaat ctgacaagct gcgctcggga aagcgtgcgt agcagaagtc
    17041 gttgccacaa cgatcaggcc aatctggtct ttctcaatgc ccgccatctc aattgcgcgt
    17101 gtcgccgctt caaagcccat ggttgaaacg gtttcgtttg gcgcggcaat gtggcgttcg
    17161 cggataccgg tacgagtgac aatccactcg tcagaggtgt ccaccatttt ttccaaatcg
    17221 gcgtttgtcc gcacttgttc gggcagatag ctgccagtac caataatctt cgtatacatg
    17281 tacgctcagt cacttttcgg ttatataccg tcacttgcaa actgcgagtt cgctggcagc
    17341 gtcctgctac cgcagagttc cgcttttgcc accgtccagc agctcaaaac cagctgggta
    17401 tacagattcc aggcgagcgg caattcgctg aggaacttgt cgctgcaccg cctgcactgc
    17461 ctgttcaatc gcgaccgcaa aagctcgctg attggctgca ccatgacttt ttatcaccgt
    17521 gccgcgcaat cctaacagac aggcgccgtt atactggtcg gggttgaggt gactgaatcg
    17581 cctcgtcagg ctcttttgta gccaacgctt taataacagt agccaccacg accgtttttt
    17641 cccttcaccc tgagatttca gcagagaaag gaacatcctg acaacacctt ccatcgtctt
    17701 taatgtgaca tttcctgtaa agccgtcaca aaccagcaca tctgtcttgc cagttaacaa
    17761 ctcattggct tcaagatagc cgatataatt gatagaaggg attgttttaa gcaccgctga
    17821 ggcatcccga atactgtcga gaccctttac ttcttcttca ccaatattga gcaacgccac
    17881 gcgaggattg ggaatttcca ccacctcttc agccagaact gagcccataa tggcaaattg
    17941 caccaacatt gtactgtcac aatcgacgtt ggcccctaag tcaaggacca ccgttttgcc
    18001 cttttgctga tgtggtaata ccgtcaccag cgccggacgc tcaatcccct ccaggggctt
    18061 gagtaataat tttgccagcc ccatcagtgc cccggtatta ccggcactga cacaggcttg
    18121 cgctcgacct tctttcacca gctccagggc cacgcgcatt gaactcccac gactggcgcg
    18181 gatagcttgc gaaggccggg catcactggc gataactgac tgcgcaggaa taatctgcag
    18241 acgcgaacgt tgttcaaagt cagctttagc aagtaatggc gtgatggcgt cgggattgcc
    18301 gactaaaaga agagtgagtt gcgaattaga attcagtgcc tgcaatgctg caggcactgt
    18361 cacggaaggg ccaaaatccc ctcccatgac atctaacgcc agggttagac gtgtcaaggt
    18421 atcgtcgccg cccggtttgg tctttcccca gttgcctggg gaaaatcctc actaagcttc
    18481 atcacgcaga cgcgtgatta cttagcgatg accttgcggc cgcggtagta accgtcggca
    18541 gtgatgtggt gacgcaggtg tttttcacca gaagttttgt ctacagacag gctggtgact
    18601 gcggtcagcg cgtcatggga acgacgcatg ccacgtttgg aacgggttgg tttattctgt
    18661 tgtacggcca tggaccttac tcctcaatta cggtttatcc ccgatccaac ggggagcacc
    18721 aattacttac gctttaagct ggctaatacg gcaaatgggt ttggcttttg cacttcttca
    18781 ggcagttcac caaagaccat gtccgcttcg gacacttcac agtgttcaga atcatgcacc
    18841 ggaactaccg gcaaggcgag gatgatttca tcttcaacca ttgcaagcag atcgatttca
    18901 ccgaattcgt taacctcaat cggttcatac gcttccggca gtgcttcagc ctgttcgtct
    18961 gaacgcacag gactaaaaca atacgttgtg tagacctgat gagtaaacgg cttcccgcaa
    19021 cgctgacact cgagcgttac cgtcaccttc gcatcgccgt ttaacactgc gagacgttgg
    19081 ttatcgatag cgaacgacat ggagcattcc acatcactgt ccacactgac tacggattcg
    19141 gcgacgcgct caacctgatc aggggtatag ataccctggt aatcaaggcg tttttgagcc
    19201 gtacgaaccg gatcgagagt caggggtaat tttacctttt gcatagggcg cgcatattaa
    19261 ctttgtaacg tcatagagtc aaagaaaaag gcagcctgtg gttgcctttt gccaataatt
    19321 cgcacacatt gcgggttact gctttatttt cgttcagtgc tgataacgaa catcaacctg
    19381 caaaacgaag cgcatagttt aaaatggcta tcatcaatac gctatatctg atggaaaaaa
    19441 atatgcctaa acttatttta gcctccacct cgccatggcg tcgcgctctt ctggaaaaac
    19501 tgcaaatctc ttttgaatgt gcagcaccag aggtcgacga aaccccacgc agcgacgaat
    19561 caccgcgaca gttggtgctt cgactggcac aagaaaaagc gcaatctctg gcgtcacgtt
    19621 atccggatca tttaattatt ggttcagacc aggtatgtgt tcttgatggc gaaatcaccg
    19681 gtaaaccgtt aacggaagaa aatgcctgtc tgcaattacg caaagccagc ggcaatatcg
    19741 tcaccttcta tactggactg gcgttgttta attcggcgaa tgggcatcta caaacagaag
    19801 tggagccttt tgacgtccat ttccgtcacc tgagcgaggc ggagattgat aattacgtgc
    19861 gtaaggagca tcccctgcac tgcgcgggta gctttaagag tgaaggattt ggcattacgc
    19921 tgtttgagcg tttagaggga cgtgacccta acacgctggt tggtttaccg ctaatcgcgc
    19981 tgtgtcagat gttacgtcgg gaagggaaaa acccgctgat gggatagttg tttgcgctgg
    20041 atgcggttac gtcgcatccg gcaatcggtg caggcggcct gatgcggcgt aaacgcctta
    20101 tcaggccaac cgcccattat caggcttata tgcttgtatt agcgcgcgtt acgcagcttt
    20161 tgcaaacaac gcttcaaacc ttcatccatc ggcgcttcga tacgcatcac ctcaccggtc
    20221 cccggatggg taaacttcaa cgctgcggcg tgcaggaaca gacgatttaa tcccgtgcct
    20281 gcttcagtga gctgtctgtc aaattcacgg tcaccgtagc gatcgtcaaa ggcaatcgga
    20341 tgacccgcat actgtgtatg cacacggatc tgatgagtac gtccggttac cgggctacaa
    20401 cgcaccaggg tggcaaatgc atagcgttct tccactttaa agcgtgtttc cgacggtttg
    20461 ccttcctgac tgacacgcac gatacgttcg ccgctttgca gaatattttt caataacggc
    20521 gcttgaacgc tcttcacatg cgactgccac tgaccgcgca ccagcgccag gtaatctttt
    20581 tgcattcctt tttcacgtaa ttgctcatgc agagaacgca acgccgagcg ttttttcgct
    20641 atcagcaaca cacctgaagt gtcccggtcg agacgatgaa ccagttcaag gaaccgcgct
    20701 tccgggcgca acgcccgcaa accttcgata acgccgaagc ttaaaccact gccgccatgt
    20761 accgccgtac cggaaggttt attcagcacc aggatatgat catcttcata taagatgacg
    20821 tccgccagcg ccgccacctt ttgcagatgt ggcgaaaccg cctcttcttc ccgctcagca
    20881 acgcgaaccg gtgggatacg cacctcatca cccgcttcga gtttatattc aggcttaata
    20941 cgttttttgt tcacccgcac ttcgccttta cgcaaaatac ggtaaatcat actttttggt
    21001 acgcctttca attgggtacg caaaaagtta tcgatacgtt gccccgcttc gtcagcggtg
    21061 atagcaacaa tttttacgga tggagtctct gttttcatgg tgggcgattc taattagcca
    21121 acaggattcg cgccactcat ttttctatgc ttatatttag ttttgcacct tattacttca
    21181 ctacgtgatc actttattga tggttattaa accaatcacc agcaagaagt gaaaaaactg
    21241 tgagtaagcg ggtgataaat ggtaaaagtc atcttgctat aacaaggctt gcagtggaat
    21301 aatgaggccg tttccgtgtc catccttgtt aaaacaagaa attttacgga ataacccatt
    21361 ttgcccgacc gatcatccac gcagcaatgg cgtaagacgt attgatcttt caggcagtta
    21421 gcgggctgcg ggttgcagtc cttaccggta gatggaaata tttctggaga gtaataccca
    21481 gtctgtttct ttgataattg cgctgttttt ccgcatgaaa aacgggcaac cgacactctg
    21541 cgcctctttg agctgacgat aaccgtgagg ttggcgacgc gactagacac gaggccatcg
    21601 gttcacaccc ggaaaggcgt tactttgccc gcagcttagt cgtcaatgta agaataatga
    21661 gtaagttacg atgaaaagaa tgttaatcaa cgcaactcag caggaagagt tgcgcgttgc
    21721 ccttgtagat gggcagcgtc tttatgacct ggatatcgaa agtccagggc acgagcagaa
    21781 aaaggcaaac atctacaaag gtaaaatcac ccgcattgaa ccgagtctgg aagctgcttt
    21841 tgttgattac ggcgctgaac gtcacggttt cctcccacta aaagaaattg cccgcgaata
    21901 tttccctgct aactacagtg ctcatggtcg tcccaacatt aaagatgtgt tgcgtgaagg
    21961 tcaggaagtc attgttcaga tcgataaaga agagcgcggc aacaaaggcg cggcattaac
    22021 cacctttatc agtctggcgg gtagctatct ggttctgatg ccaaataacc cgcgcgcagg
    22081 tggcatttct cgccgtatcg aaggcgatga ccgtaccgaa ttaaaagaag cactggcaag
    22141 ccttgaactg ccggaaggca tggggcttat cgtgcgcacc gctggcgtag gcaaatctgc
    22201 tgaggcgctg caatgggatt taagcttccg cctgaaacac tgggaagcca tcaaaaaagc
    22261 cgctgaaagc cgcccggctc cgttcctgat tcatcaggag agcaacgtaa tcgttcgcgc
    22321 attccgcgat tacttacgtc aggacatcgg cgaaatcctt atcgataacc cgaaagtgct
    22381 cgaactggca cgtcagcata tcgctgcatt aggtcgcccg gatttcagca gcaaaatcaa
    22441 actgtatacc ggcgagatcc cgctgttcag ccactaccag attgagtcgc agatcgagtc
    22501 cgccttccag cgtgaagtcc gtctgccgtc cggtggttcg attgttatcg acagcaccga
    22561 agcgttaacg gccatcgaca tcaactccgc acgcgcgacc cgcggcggcg atatcgaaga
    22621 aaccgcgttt aacaccaacc tcgaagctgc cgatgagatt gctcgtcagc tgcgcctgcg
    22681 tgacctcggc ggcctgattg ttatcgactt catcgacatg acgcctgtac gccaccagcg
    22741 tgcggtagaa aaccgtctgc gtgaagcggt gcgtcaggac cgtgcgcgta ttcaaatcag
    22801 ccatatttct cgctttggcc tgctggaaat gtcccgccag cgcctgagcc catcactggg
    22861 tgaatccagc catcacgtct gcccgcgctg ctccggtacc ggtaccgtgc gtgacaacga
    22921 atcgctgtcg ctctctattc tgcgtttgat cgaagaagaa gcgctgaaag agaacaccca
    22981 ggaagttcac gccattgttc ctgtgccaat cgcttcttat ctgctgaatg aaaaacgttc
    23041 tgcggtgaat gcgattgaaa cgcgtcagga cggtgttcgc tgcgtgattg tgccaaacga
    23101 tcagatggaa accccgcact accacgtgtt gcgcgtgcgc aaaggggaag aaacgccgac
    23161 cttaagctac atgctgccga agctgcatga agaagcgatg gcgctgccgt ctgaagaaga
    23221 gttcgctgaa cgtaagcgtc ctgaacaacc tgcactggca acctttgcca tgccggatgt
    23281 gccgccagcg ccaaccccag ctgaacctgc cgcgactgtc gtagccccag caccgaaagc
    23341 tgcaacggca acaccagcag ctcctgcaca acctgggctg ttaagccgct tcttcggcgc
    23401 actgaaagcg ctgttcagcg gtggtgaaga agccaaaccg accgagcaac caacaccgaa
    23461 agcagaagcg aaaccggaac gtcaacagga tcgtcgcaaa cctcgtcaga gcaaccgccg
    23521 tgaccgtaat gagcgccgcg acacccgtag tgaacgtact gaaggcagcg ataatcgcga
    23581 agaaaaccgt cgtaatcgtc gccaggcaca gcagcagact gccgagacgc gtgagagccg
    23641 tcagcaggct gaggtaacgg aaaaagcacg taccaccgac gagcagcaag cgccgcgtcg
    23701 tgaacgtagc cgccgccgta acgatgataa acgtcaggcg caacaagaag cgaaggcgct
    23761 gaatgttgaa gagcaatctg ttcaggaaac cgaacaggaa gaacgtgtac gtccggttca
    23821 gccgcgtcgt aaacagcgtc agctcaatca gaaagtgcgt tacgagcaaa gcgtagccga
    23881 agaagcggta gtcgcaccgg tggttgaaga aactgccgct gccgaaccaa ttgttcagga
    23941 agctccagct ccacgcacag aactggtgaa agtcccgctg ccagtcgtag cgcaaactgc
    24001 accagaacag caagaagaga acaatgctga taaccgtgac aacggtggca tgccgcgtcg
    24061 ttctcgccgc tcgcctcgtc acctgcgcgt gagtggtcag cgtcgtcgtc gctatcgtga
    24121 cgagcgttat ccaacccagt cgccaatgcc gttgaccgta gcgtgcgcgt ctccggaact
    24181 ggcctctggc aaagtctgga tccgctatcc aattgtacgt ccgcaagatg tacaagttga
    24241 agagcagcgc gaacaggaag aagtacaagt gcagccgatg gtgactgggg tccctgtcgc
    24301 cgccgctgtc gaacctgttg ttagcgcacc ggttgttgaa gaagtggctg aagttgtaga
    24361 agcccccgtt cagattgccg aaccgcaacc ggaagtggtt gaaacgacgc atcctgaagt
    24421 gattgccgcc gcagtaactg aacagccgca ggtgattacc gagtctgatg ttgccgtagc
    24481 ccaggaagtt gcagaacacg cagaaccggt ggttgaaccg caggaagaga cggcagatat
    24541 tgaagaagtt gccgaaactg ctgaggttgt ggttgctgaa cctgaagttg ttgctcaacc
    24601 tgccgcgcca gtcgtcgctg aagtcgcagc agaagttgaa acggtagctg cggttgaacc
    24661 tgaggtcacc gttgagcata accacgctac ggtgccgatg acgcgcgctc cggcaccgga
    24721 atatgttccg gatgcaccgc gtcacagtga ctggcagcgc cctacttttg ccttcgaagg
    24781 taaaggtgcc gcaggtggtc atacggcaac acatcatgcc tctgccgctc ctgcgcgtcc
    24841 gcaacctgtt gagtaataat tagctcaagt aatcaagccc tggtaactgc cagggctttt
    24901 ttatttcatc tttgaatcaa catattaaaa ttcatcgaat ggcatccttg ctaaccaaca
    24961 atgcaaaata ggcaaaaaaa tgcccgcccc ggcgatccca gagcaggcag acaaaacata
    25021 cccagtttca tgatatgttt taaagcgaaa ttatttgttg agctggaaga gcgacaatcc
    25081 ctgcatatcg gtaaatgctt tatacgatgc ctgcaatgcc gtttgctgca tgatgtaaga
    25141 tgaaatagtt gcattccagt ccacatcaac cagatcgctc atctgctgcg tttgccctaa
    25201 agcgcgatcg ctacctaatg aatccagcga ctccagttcg ttcagctgcg tgcctaattc
    25261 cgcgcgaacg gtcagcacat tgttcagcga gtttttcagt ccgcggttgg ttttatctaa
    25321 cgccgcagcg gcggtttctt tatccgcttc gctatccgcg accggcgttt tcagggctgc
    25381 gatggcgcta tccagcatgg caaaaagatt ggtttcagaa gcgctaccgt ctggttccgc
    25441 taccgcgtta ctggtaatac tgtcgaaaat tttgtcgccc gtatgtccta tcaccatcga
    25501 acgcgaagca tcgacctgtt gcttaatact ttctgttccg ccgacataat caccgttagc
    25561 ctcactaaac ggcgcagtct ctgttttata accggcaaaa atgtagcgcc cattaccgtc
    25621 agtggtgttc gccagattca gcaactggtc acgaagcccc tgaatatccg tagccagcga
    25681 ggcccggtca tcgtcactca aggtgccatt gctggcgtag acaatttttt cctgagcatt
    25741 ctggatagca gtggtgacct ggctaagtac actctcttcc agtgacactt tttgagtggc
    25801 gaaagtacgc gccagcgtgt actggctgtt ttgcgcctgt gcctgggaga gaacaaccgc
    25861 ttgtgatgca gcaatgggat catctgaagg gttaacgact cgcttacccg tcgacatctg
    25921 ttcgccgtac ttcatccatt ctgcctgaga attggtgata ccacgcatgt tttgctggta
    25981 catcatctgg gtactgaaac gcattttatc tccccttagc gaatgttaat cagcgcatca
    26041 aaaatcgcgt ttgccgtctg cagaacctgc gcattcgcca ggtaatactg ctgaaaacgt
    26101 tgcagatttc cgtactcctc atcgagattg acaccggaaa tcgactgctg ctgattggaa
    26161 agctgcgtca caacattacc ttgcgtggtg ctactggttt tcaacgtcgc ggttttatta
    26221 ccgatatcac tcactaacga ggcataagcg tcgttaaagg atttcgcacc gcccaccgtt
    26281 ttactgttgc tttgcagatc cagcagggcc tgaccgttgc ggttatcgct atcacccgca
    26341 tctttttcgc tcgccatcgc tattttcgct tcgtcggtga ttaatacatc catgttgacg
    26401 atggcgtcac ttactggttt cagcgtgaag ctgtcgttaa cggcaggcgt tcctgtaaac
    26461 gtcaactcca gaccatcaaa tgccacttta ccgttggcat ccggcgtcac cgtaaaagtg
    26521 gtattgctgg caaggcgggt gacctgccac tgattattat cgaacgagat tttgtaatct
    26581 gtcgccagta ccacggaggc atcagttacc gtggcaccga tcgcaacgtc acctttgttt
    26641 ttcgtgtttt gcagaaccgc gggcttaccg atagtaaaga aatcttcacc agcatcgccg
    26701 ttagcatcaa atccggcttt gtgttgggtg ttgaaagcct cggcaaatgc cagcgccagt
    26761 tgtccaagcg tattacgcgt ctggtccagc tcctgagaac ggaatgtcag aatgccgccc
    26821 agcgacccgg tattcagtaa tttctccggg atctcaatat tgcctgccgt cccatcaaca
    26881 taagcgacag tcgtacgaga agggtcagcg ctggaaggaa ctgccgccag ttgccgcgcc
    26941 gtacttccct gaaccagtga gtaaccattg gccaccgtga tgttataagt gccgccatcc
    27001 tgaacgctga cttctacacc aacaatctgg tttaattcgc tcaccagttg atcgcgttga
    27061 tccagcagat tgttaggtga cgcccctgcc cccacgcctg tcaggcgcga gatttgatcg
    27121 ttcaggctgg caatttgttt agcgtaattg ttgatctgat caacgctggc acctatcgcg
    27181 atattgacct gtttgtcctg gtcgcgcaga tattgatcgg tggttttaaa ctgattcacc
    27241 aacccttctg atttcccaat cagcgcctgg cgcgctgccg ggtcttccgc gttactcacc
    27301 agcgtttgca ggctggtgaa gaaatcctgc atctgtgttg ccagcgaaga ggtactggtg
    27361 gagagcatat tgtcgatttt cgacatctgc tcatagcggg cggtcaggcc gctactttgc
    27421 gtctgcgcag cacgtaactg gttggtaata aacgcatcat actcacgctg cacaccagaa
    27481 acgtagacgc cattgccaac ccagccgcca gcgcccaacg tgctattggc ctgcgccata
    27541 atagtggttt ggcgggtata tccggcaacg ttatagctgg agatattatt acttgccgta
    27601 tttaacgccg cctgggccgc gttcagtccg ctcatggcgt tattgatcaa gctggacatg
    27661 gaggttcctt ataagccttc aattacagag tattatcggc agcgacccgc cggacttgag
    27721 ttattcagaa cagattatca atgttcatgc tgtaggtttt gctcaccttg tcgcttatcg
    27781 atttcatctg ctggatcatg ttggtgagtt tacgggcata gtgaggatcg gtagcatacc
    27841 ccgcgtcctg tagggcctgc gccccctgct ccgcactcgc ggctgtcgtc acggcggcgt
    27901 agcgcgggtt acgtgttaac agcccaacgt aatccgacaa tgcttccaga tacgagctgt
    27961 agacccgaaa tttcgctttt actttcttcg cttcgccatt ttcatattca gtcgtggtga
    28021 tttcagtaac tggacctttc cagttgccag aggctttgac gccaaacagg ttatagctcg
    28081 gctcgccgtt ttcgcggcgg atttgccgtt gcccccaacc agactccagc gccgcctgag
    28141 cgaggatcaa atgatgtggc acaccgcttt gctggcttgc cagttgggcg ggcagcgaga
    28201 gttgcgcgag gaatgcttta ctgtcacccg gcagcgaatc atcgtagtta cgtggcacgg
    28261 ccttttgcac cagctgcgaa agcgcctgat tttgataacg caccacagtt tcgagcggga
    28321 atttcatcgg tgctgctggc gtggactcct ctggcaatgg ttgttctggc gtcatctgtt
    28381 taaccatcat ctctgcaagc cccagacctt tgcccgccgt catctgttgg gcaatctgct
    28441 ggtcatacat actggtatac aggcgagtgt gctcgctgct gaacaggcca tcttttggta
    28501 aagcgtcgcg catgcttttc aacatcatct gcacgaacat cccttccacc tgacgggcca
    28561 ccggacggat atttgccgcc ggatcttcgc ccgctttcgc ctttagttcg ttgagtgatt
    28621 gcgcatccca ggccgcactt gccagtagtt tgctgtcgct gatcatcaga tgatttccag
    28681 ttttgcccgc agacatcccg cactttgcat tgattgcagt atggacatca gatccatcgg
    28741 cgtagcgccc agcgcattga gcgcgcgcac cacgttattg aggctggcgc tggaacgtac
    28801 gctttgcagc gaaccgccgc tctggcgtaa atcgatctgc gtttgtggag taaccacagt
    28861 ctgtccacca ccaaacggtg tatctggctg gctgacattg gcctgacgat tgactgttac
    28921 tgagagattt ccctgcgcta ccgcgcagct gtcgagggtc acttcgcgat tcatcaccac
    28981 cgaaccggtg cgcgagttaa tcactacttt agcgtcctgc ggggtgacat taacctgcat
    29041 attctggata tctgcaagga aacggacctg ggaactgttg ccacttggta cgcgcacctg
    29101 aatagtccgc gcatctaacg cggtggcgct gccatatcca cgcacgcggt tgatggtgtc
    29161 agcgatttgc tgcgccatgc tgaaatcttc gtcgttaagt tgcaaattaa gggtattccc
    29221 gacgccaaac tggctgggca attcacgttc aataaccgca ccattggtga tccgtccacc
    29281 gttcagttgg ttcacctgaa cactgctacc gccagcggag gctcctgcgc cgccaaccag
    29341 aatattgccc tgcgccagcg catacacctg actgtcaacg cccttaagcg gcgtcatcaa
    29401 caacgtacct ccacgcaagc ttttggcatt tcccatggaa gaaaccacca catcgatggt
    29461 ttgcccctga cgtccaaacg gaggaagtga ctctgtcacc attaccgcag cgacgttttt
    29521 tagctgcata ttggtgcccg tcggaacggt aattcccagc tgtgagagca tgttattaag
    29581 cgtttgtgtg gtaaacggcg tctgggttgt ctggtcaccg gtgccatcca gccccaccac
    29641 cagaccatag ccaatcagtg agttttgcct taccccctga acactggtga gatcgcgaat
    29701 acgctcagcc tgagccgccg tcgtaaccag tagaagaatt aatgcagaga gaaatttaat
    29761 caccacagcc tcacttacat tggcgacagg ttaaggaaga aacgctgcaa ccagcccata
    29821 ttttgcgctt cgttaatgta gccattgcct acgtattcaa tgcgcgcatc cgccacctga
    29881 gtagacggta cggtattgct gccgctgata gtgcgtggat taaccacgcc agagaagcga
    29941 ataaattcgg taccctgatt aatggcaatc tgtttttcac ccaccacatg caggttgccg
    30001 ttgaccagta cctggtcaac cgtcaccgtc aacgtgccgc taaaggtatt gctggcattg
    30061 gccccgccct ttccgttgaa cgtgttacca ccggaggctt cgacatcggc acgagcgtta
    30121 ccaaacagcc cctgcaaata gcgcggcaca gtatcaaagc caaaattagt tttaccgtca
    30181 cggctggcat tcgcagagga gcttttgctg gcgctgacgt tctcctgcaa cacgatggtc
    30241 agcgtatcgc caatattgcg tggtcgacga tcttcaaaca gcggttgata gccatagtta
    30301 atcggctgag cagactggaa aatagaaccg ttggcgacgg gcgtcggacc gggaaccggt
    30361 tgtgcactgg tcgccccctg caccagcggc gtggagggta tccaggcgca gccggttagt
    30421 gaaagcacca acaagctgga aatggcataa gtatgcgcag cgtttttttg catggctatc
    30481 atcttcaaaa atcagtaaac cggtgaacct gccaccggtt aagccttaga gttgcgtcag
    30541 tttttgcagc atctgatcgg tggtggacac cgctttactg ttgatttcgt aagcgcgttg
    30601 cacctgaatc atattgacca gttcttccgc cacgttgacg ttagacgttt caacataccc
    30661 ttgatacagc agtcccgcgc cgttcaggcc cggcgtgctt tcgttcggta caccagagga
    30721 ttgcgtttcg gtgtagaggt tttcgccaat gctctccagc ccggtgtcat tcataaaggt
    30781 ggtgagattg agctgcccaa cctgaaccgg agctgcctgg ccttgttggg ttacgctgac
    30841 cacgccatca cgaccgatgg tgatacttaa cgcattcgcc ggaatggtga tcgctggctg
    30901 cacctgaaaa ccaccagctg cccgttctga tccacctgga aagagccgtc acgggtatag
    30961 gctgatgaac catctggcaa catcacctgg aaaaagccct gccctttaat cgcgacatct
    31021 ttgctgttgt tggtctgcga caggtttccc tggctgtgta agcgttcagt ggcgaccggg
    31081 cgtacccccg tgccgatttg taatccggag ggtaaggtgg tttgttcgga agactgtgcc
    31141 cccggctggc gaatggtttg ataaagcaga tcttcaaaca ccgcgcgctg acgcttaaaa
    31201 ccgttagtac tgacgtttgc caggttgttg gcaatgacgt ccatattggt ttgctgggcg
    31261 tcaaggcccg ttttggcgat ccataatgaa ctgatcataa aatgtcctgt agtggattag
    31321 tgacaaagtg catatgtctt cttgccggat gcggcgtaaa cgccttatcc ggcctacaag
    31381 tgctcgcaaa atcgataaat tgcgattgcc cccatagacc cgataaactt gcgcatcggg
    31441 caacttatac ttgtcatgta tcctttcaat taactcatcg acagcagttg gttggcacgg
    31501 cctgcgttat catcgacgct gctgatcacc ttcatctgca tttcaaaacg ccgcgcgctg
    31561 gcaatcatgt cgctcattgc cgcaacggca ttgacgttac tgccttccag aacccccgac
    31621 atcacacgca aggttggatc tgcctgcagt accggccctc gcgtggcctg ggtttctgcg
    31681 cttaaacgaa aaatgccgtc gtcaccgcgc tgcacttcgc tgcccgtggc tttcaccagt
    31741 ttcagacgcc ctactggcgc aactgtattt gccggatcgc ccggattgag cgccgagatt
    31801 gtgccatcgg cagcaatagt gatttccgct ccttccggca cagcaattgg ccccgcctcg
    31861 cctatcacct gatgcccctg aattgtcagt tgcccggtgg gatcaacctg aatgctgcca
    31921 ttacgcgtat acccttcgct gccgtcagcg gtctgcacgg ccagccagcc atcctgctgc
    31981 aacgcgacgt ccagcgggcg cgaggtgtaa tccattttgc cgggcgtcat atctgcgccc
    32041 ggcgttgacg ccgtgaccaa cgtgcgcgtg ggcagagaaa gcccttccac tggcaccgcg
    32101 cgtaaagcat tcaactgcgc gcgaaaaccg ggcgttgagg cattggccag attactggcg
    32161 gttaccgcct gttgattcag tgtctggctg gctgctccca tcgcggtata aattgcgtga
    32221 tccattgagc tatcccgtca gcgattagcg taagttaacc agcgtgttga ggatctggtc
    32281 ctgagttttg atggtctggg cgttagactg atagttacgc tgggcaacga tcatattgac
    32341 cagttcttta ctgagatcga cattggacgc ttccaacgca ccgttggtca gggtgccaaa
    32401 gttgcccgtc ccggctgtcc ccaacagcgc cacgccagaa gattgcgtcg cagaccagac
    32461 gttgtcgcct tcggatgcca gaccttcgtt gttggcaaag ttcgccagta caatctgccc
    32521 cagcagttgt gtttgttcgt tggaatagtt gccgacaacc gtaccgtcat cattgatttg
    32581 ataactcacc agatcgcccg gtttgtagcc attctgggtg gttgccacaa tattgttagc
    32641 gccggtattt tgctgcatgg agttgaggaa gctcaggcta aacgtggcgg gatctgcgcc
    32701 gttaattgcg ccagtggtaa tattctctgt tggatttgag gtcagaacgc cattggcatt
    32761 aaacaccagc ttcattgcag gctcggctgt acctgttgga tcactgctat cctgggtgta
    32821 gacctgccag ttattatccc cggtcttcac aaagtagacg ctcatgtcat gagcattacc
    32881 ctgactgtcg aaaacagtca ccgaaccttt tttgttatag ctatccgcat tgctggcgct
    32941 gaatggcgta acagtaggaa gcggatcact ggaattcagg ttgatctgca tcgacgccgt
    33001 ggtggtagtt ttcgctgcca tcagggtatt cgggatcgaa atattggtcg gattcgcccc
    33061 ttgctgaata gtcggcggcg taccggttgc cgggtaaccc gtcagctgta aaccttgcat
    33121 attcaccagg ttacggtttt catccagctt aaattgtccg ttacggctgt agaacaccga
    33181 accgttgctg tctaccagac ggaaaaaacc gttctggctg atagcaacgt ccagacctcg
    33241 cccggtgttg gtggtcgtgc catcggtaaa gtcctgagtg ataccggcaa cttttacccc
    33301 cagtcccact ttcgaaccgg caaacatatc ggcaaaagag gccgtgcctg atttaaagcc
    33361 gtaggtggcg gagttggcga tattgttgcc aataacatcg aggttggtgg cggcagcgtt
    33421 taatccgcta accgcttgag aaaaggccat gactgactcc tgaaatgtga aggcttaaat
    33481 tatctgccgt acttcgtcga gggtggtggt gccgtaagtg ccgagatcca gcgtattacc
    33541 gctgttgccg cggatcacac cctgcaccag agcaaactgc agcggctggg caaccagttg
    33601 tgtaccaccg ttactggcgc taatcgctac attgtaagaa ccgttcggcg cagtgctgcc
    33661 atcagtcaac gtaccgtccc aggtgaaact gtggactccg gcggtcagtt caccaatatc
    33721 aatggtgcgc acaaccgcgc catttttatc ggtgatggtg gccgtaactt tgtctgccgc
    33781 ctgttgcagc tcaacaccaa acggcgtggt cgtggtcaca gccccttctt cactgccggt
    33841 tcccgcaaga acagtggtgc cggggatcat cacgccgtga ccgatcaggt tactggcctg
    33901 taacgactgg ctgttatcaa tctgtccgga aatagatccg agcgtggtat tgagtttttc
    33961 aatcccgctg accgtgctga tttgtgccaa ttgcgacgtc agctcgttgt tttccattgg
    34021 attggtcggg tcctggtttt tcagctgcgc caccaacaaa gtcagaaaac tgctttgtaa
    34081 gtctgcggcg ttgctgcccg tgagcgaact actgctggtg gtactgacgc cggtatttgt
    34141 cggatcggtg gtggttaccg caatggacat agctttctcc tttattgacc gagcgtaagg
    34201 gttttcagca tcatgctttt caccatgttg agcacttcaa cattggcctg atagctgcgt
    34261 gacgccgaca tggtgttaac catctccccg acgacatcaa cgttcggcat ttttacgtag
    34321 ccctttgcat ccgccagcgg attacccggt tcataaacca gtttgtccgg ggcctgactt
    34381 tctataacat cggcaacctt tacgccgcct gtcgcagcac ctggtgcagc gttaacctgg
    34441 aataccacct gttttgcccg atatggctgt ccatcgggac cggtcacgct atcagcattc
    34501 gccagattac tggccgccac gttcaagcgc tgggactggg cagttaacgc cgacccggcg
    34561 atatcaaaaa tattcagcag tgccatccgt taatttccgc tctgtaaaac gttcatcatg
    34621 cctttgattt gcccgctcaa cgcgctaagg ctcatctggt attgcaggct gttatcggca
    34681 aactgggtgc gttcgcgatc catatcgacg gtattaccgt caagcgaagg ctggtccgga
    34741 atacggtatt gcagttctgc ggtaggaggc gtcagcgcct gcgccggaat gtgttgcgtt
    34801 gaggtcatcg tcagtgcaac cacactggtt gcatcccgtc cacgttgcat gactttttta
    34861 agttcactgg caaaatcgat atcgcgcgcc tgataaccag gggtatcggc attggcgatg
    34921 tttgctgcca gcacttcctg acgctgggcg cgcagattga gcgcctcttg ttgaaaacgt
    34981 aaggcggcgt cgagcttatc gagcatatct cctccgcagg tatcaaaatt ctgccatcag
    35041 cttaaatgcc tttacgcgcg cgttatcggc ggaataaacg caaaatgggt cgctatttat
    35101 gccgttgatg accattgcgg acaggtacaa ttcacgttgt agaaatggct gggggcgaaa
    35161 atgctggcaa taaaacgtag cgtggcgatc atctcgatac tattcactcc gttaagtgcg
    35221 gcgagtaatc tcacatcgca attgcaaacc ttttttagcg cccagctcgc gggggtaagt
    35281 gatgaggttc gtgtttctat tcgtacagcg cccaatctac taccgccgtg cgagcagcca
    35341 ttgctttcga tgagcaataa ttcccgcctg tggggcaatg tgaatgtgtt ggcacgctgc
    35401 ggtaacgaca aacgatattt acaggttaat gtacaggcca caggaaatta tgtggttgcc
    35461 gcgatgccca ttgtgcgggg aggaaagctg gaagctggca atgtcaaact aaaacgcgga
    35521 cggcaggata ccctgccacc gcgtacggtg ctggatatca atcaacttgt tgatgccgtt
    35581 agcctgcgcg atctatcacc cgatcaacct atccagttaa cccagtttcg ccaggcatgg
    35641 cgggtaaaag cgggacaacg cgtcaacgtg atcgccagcg gtaacgggtt tagcgccaac
    35701 gcagaaggtc aggcgctgaa caatgcagcc gtcacacaga atgcgcgggt gcgcatggta
    35761 tcgggactgg tggtcagcgg cgttgttgat gcagatggga atattcttat aaaccagtaa
    35821 gctgttaaag attacccgtc ccttgccgat aaataagcaa cacatgataa aagcgccctc
    35881 aatgaggaat aaaccatgag tattgatcgc acttcgcctc tgaagcccgt aagcaccgtt
    35941 caaccacgcg aaaccactga cgcgtcggta acgaacaccc gggcggcaaa aacaacagcc
    36001 tccaccagca ccagtgtgac gttaagcgac gcgcaagcaa aactgatgca acccggcagc
    36061 agtgatatca atcttgaacg tgtcgaagcg ttaaaactgg cgattcgtaa cggtgaacta
    36121 aaaatggaca ccggcaaaat tgccgatgcg ctgatcaacg aagcgcagca agacttgcag
    36181 agtaactgat cgtatgacac gtcttgcaga gatcctcgac cagatgtccg ctgtgcttaa
    36241 cgatctcaaa acggtaatgg atcaagaaca gcaacatctc tctatggggc agatcaacgg
    36301 cagccagttg caatggatta cagaacaaaa aagctcactg ctggcgacgc tggattacct
    36361 cgaacagtta cgcaggaaag aacccaatac agcaaatagc gttgatatta gtcaacgctg
    36421 gcaggaaatt actgtgaaaa cgcagcaact acgccaaatg aatcaacata acggctggtt
    36481 actggaagga cagattgagc gcaatcaaca ggcgctggaa atgttgaaac cgcatcagga
    36541 accgacgcta tatggggcga acggtcagac ctcaacaacc catcgcggcg gtaaaaagat
    36601 ttcgatctga atattatctc cggcctgcac tgcaggccgg aatgcattgt tacaccgtcc
    36661 ggcgggcaaa ttctttaact ttgaagccca gtaccgccag tgcagcgaag tacgcggcaa
    36721 tccccgccag cacgaccgcc attaaacgca gtaagcgcca aggcatggta cccaatgacc
    36781 actccggcat gatatgtaac atacctaaaa gcacgccaga catcaccagt accgccacca
    36841 ccagacgcaa cagaaacgcc atccagccgg gttgcggagt aaagattttc tgcttacgca
    36901 actgccagta aagcagcgaa gcattcagac acgccgccag accaatagaa agtgacagcc
    36961 cggcatgttt caacggacca ataaacgcca ggttcatcaa ttgcgttaaa atcagcgtaa
    37021 cgatggcaat tttcactggc gttttaatgt cctggcggga ataaaagcca ggagccaaca
    37081 ctttcactac aatcaggcca atcaaaccca ccgagtaggc aattaacgcc cgctgggtca
    37141 tcagcgcatc aaacgcggta aatttaccgt actggaacag cgaaacggtt aacggacccg
    37201 aaagaatgcc caacgcaacc gcactcggca gcgccaacag gaaacaaaga cgcaaccccc
    37261 agtccatcaa acggttgtat tcatcatgat tgccactggc aaaacttttc gacagcgacg
    37321 gcagcaaaat ggtgccaagc gccacgccca gcacaccgga gggaaactcc attaagcggt
    37381 cggcgtaata catccaagac accgaaccgg aagcaagaaa cgaggcaaaa atggtgttga
    37441 tgattaagga gatctggctc acagagacgc caaggatcgc cggtcccatc tgtttcacca
    37501 cgcgcattgc tccggcatcg tggaagttaa tgcgcggcag gaccagcatg ccgatcttct
    37561 tcaggtgcgg tagctgatac accagctgca ggatgccgcc gaccgtaaca gcccacgcca
    37621 gcgccagcac cggtgggtta aagtacggtg cggcaaacag cgcgaaacca atcatgctga
    37681 tgttaagcag tgttggcgca aacgccggaa tcgagaagcg gttccacgta ttcagaatcg
    37741 ctcccaccag cgacgccagg gagatcagca agatataggg aaaggtaatc tttagtagct
    37801 ggctggtcag ggcaaatttg tcagctgtgt cagcgaagcc tggcgcggtc accatgatca
    37861 cccacggtgc ggcgagcatg ccagcgaccg tcacaaccgc cagcgcaagt gtcagcaggc
    37921 cagaaacata agagacaaag acccgcgtgg cgtcttcacc ctgcttactt ttatattccg
    37981 ccagaatcgg tacaaatgcc tgggaaaatg ccccttcggc aaagatacgg cgtaacaagt
    38041 taggaagttt aaaagcgacg aaaaaggcgt cggttgccat ccctgcgcca aagattctgg
    38101 cgacaattgc gtctcgtgcg aagccaagca cacgcgaaaa catggtcatc gagctgacgg
    38161 cggccagcga ttttaataaa ttcatcggtg ttctaatcca gacccacggg caaaacgcct
    38221 gcaaggcagg cgctgaaaga ggcgaatagt ctacctggat tatggtgaat tgctaccgcc
    38281 agatgttaca gggtttattc actcatcgca tcgcgccaga tcttgtcaac gatacgttgc
    38341 gccagcactg cctgttcgcc ggcggtttgc agaactgtct gattttgcac acattcaatg
    38401 aagtgccgcg cacagccgac aaatccacgt tgctcaagag tgctctgcca accaggaatc
    38461 ggtttatgca ctacgccctg cccgcgctcc tcacgccatt cgcgcatatc cgtaatgtcg
    38521 atgagcgcac cgtcagtcac ggcctgcacg gtttcacgct gacttccggc acggcgatgc
    38581 atacaggtgg tgatctgcaa aggatcggcc gaaaaatggt gctcggcaaa cagcatttcg
    38641 ccagcgtcgt tagtcagtag cgtaccgcca tccagagagg ctttgccgcc cgacaaccac
    38701 agcgcggtat ccaccacatg cagataatca tccagcaacg tgaaataaag atcgtgtggc
    38761 ccgacactat tgctacgatg tttatccatt cttagcgaag ctgcggtagc gagttgcgtt
    38821 tttaactcac cgtagagtgg tgcgaaacga cggttaaaac cgaccatcaa cgtcagtttt
    38881 ttccgcgccg ccagttccac cagccgttca gcatcgcgca gattttctgc cagcggttta
    38941 tcgacacaga catgtacacc cgcattgagt aacgtactga ccacgtcaaa gtggctggcg
    39001 gtgctggaat gcacaaaaac cgcatcgcaa ctggcggcaa ggctggataa cgaatcggca
    39061 taaggaatgc gccagctttc acaaattggc agggctttcg cgcgcgtagg cgaccaggct
    39121 ccttgtaaca tccagtcaga cgctgccgcc agcaccggta accacgcttt ttgcgcaatg
    39181 ccgcctaatc ccacaacgcc gatacgtaat tttttcactt taatctccca gatgggccag
    39241 caacgaatca agacgctgtt tcagttctgc cacttcgatt tccagggctt cgacgcgggc
    39301 ctgtaaatca ccatcaaccg cgttcgacat atccgttacc gccggctgat cctctacctc
    39361 accactgaaa agatgcatgt agcggctttc gcgtttaccc ggttcgcggg ccagacgcac
    39421 cacaaaagga ccatcttcgc gatttgccag ttgttccagc gtcgactcca cttccgccat
    39481 atcgctgaat tcatacattc gcgcggcgcg gctgcgcagt tcacccggcg tctgggcacc
    39541 acgcaataac aacgtggtga ttaacgccac ttccgctgcg ctcagtttca gatcgccaaa
    39601 ttctgaatta caaaaacgtt gttcatattt ggtgacccga ttaccaaagc cactcaccgt
    39661 gcgcagataa tggcgtttga ccagattatc cagttgctcc tgcacttcag attcactcag
    39721 attcatgacc ggttcacggt tcgttttctg attacaggcc gtgactacac cattgactga
    39781 gagcgggtat tgctccggcg tggtcacctg cttttccagc aagcaaccaa tcacgcgagc
    39841 ttcaagggca gtaagttgat atttcatgag attctcctta gcggccgggc gtccagtctg
    39901 gggtagttaa tgccgtcagt acgtgatcgc gccattgtcc atcaatcaac agatagtctt
    39961 tcgcatagcc ttctttttca aaacccagtc gcgccagtaa atcaccgctg cgtttattgt
    40021 gcggcatata attagccata atgcgatgaa tatgttgggt gcgctgcata taacgaatgg
    40081 ctgcggtcag ggcttcaaac atgagtcctt tgccctgcca tttttgccca atcgaataac
    40141 cgagatagca ggcatgaaaa gagccacgaa caacattgga aaaattggca acgccaataa
    40201 tctctttttc gtccgggtcg aataagccaa agtagaaagc tgaaccttgt ttatgaaatt
    40261 cgttaatcat ccccagcctg gcctgccagc ctgatggata acagtggctt tcgtcgcgca
    40321 ctggctccca gggcttgagg aaatggcgat tctctgcgta ataatccgca agacgccagg
    40381 catcacgatc atgcaccaga cgcacgacca gtcggtctgt ggttaagcgc acttttggca
    40441 cgttactgcg atagccaaac attctatacc tactccttcc cgtaacgtct ttaatacgct
    40501 gtaagtatta ctatacctgc gcaaccagac cctgtgaaaa gtccaaaatg ccattttctg
    40561 cattattcac atatttgata aaaactctct atcaaagcga cgttttgata aaaaaatatt
    40621 gtccccgaag tgatgggaaa agtcttagcg gcaatgcaga atagaaagtg ctcatctttt
    40681 atttcccggg aggggaaatg tcccgcgtat cgcaggcgag gaacctgggt aaatatttcc
    40741 tgctcatcga taatatgctg gtcgtgctgg ggttctttgt tgtcttcccg ctgatctcta
    40801 tccgcttcgt tgatcaaatg ggctgggccg ccgtcatggt cggtattgct ctcggtctac
    40861 gccagtttat tcagcaaggt ctgggtattt tcggcggcgc aattgccgac cgctttggtg
    40921 ccaaaccgat gattgttacc ggtatgctga tgcgcgccgc cggattcgcc acaatgggta
    40981 tcgcccacga accgtggcta ttgtggtttt catgcctgct ctcgggactc ggcggcacgt
    41041 tgtttgatcc gccgcgttcg gcgctggtgg tgaaactaat ccgtccacaa caacgttgtc
    41101 gttttttctc gctgttgatg atgcaggaca gtgccggtgc ggtcattggc gcattgttgg
    41161 ggagctggct gttgcaatac gactttcgcc tggtctgcgc cacaggggcc gttttgtttg
    41221 tgctgtgtgc ggcgttcaat gcgtggttgt taccggcatg gaaactctcc accgtacgca
    41281 cgcccgttcg cgaaggcatg acccgcgtga tgcgtgacaa gcgttttgtc acctatgtcc
    41341 tgacgctggc gggttactac atgctggctg tacaagtgat gctgatgctg ccaattatgg
    41401 tcaacgacgt ggctggcgcg ccctctgccg ttaaatggat gtatgccatt gaagcgtgtc
    41461 tgtcgttaac gttgctctac cctatcgccc gctggagtga aaagcatttt cgtctggaac
    41521 accggttgat ggctgggctg ttgataatgt cattaagcat gatgccggtg ggcatggtca
    41581 gcggtctgca acaacttttc accctgattt gtctgtttta tatcgggtcg atcattgccg
    41641 agcctgcgcg tgaaacctta agtgctttgc tggcggacgc aagagctcgc ggcagctata
    41701 tggggtttag ccgtctgggt ctggcgattg gcggcgctat tggttatatc ggtggtggct
    41761 ggctgtttga cctgggcaaa tcggcgcacc agccagagct tccgtggatg atgctgggaa
    41821 ttattggcat cttcactttc cttgcgctgg gttggcagtt tagtcagaaa cgcgcaacgc
    41881 gtcgtttgct ggaacgcgac gcctgatttg atactccccc ctgactcctc catactgaaa
    41941 gtgaactgac agccaaatgg aggagtcacg tgaagctata catttacgat cactgccctt
    42001 actgcctcaa agcccgcatg attttcggcc tgaaaaatat ccccgtcgaa ttacatgttc
    42061 tgctcaacga cgacgcagaa acacccaccc ggatggtcgg tcaaaaacag gttcccattc
    42121 tgcaaaaaga tgacagccgc tatatgccag aaagcatgga tatcgttcac tatgtcgata
    42181 aactcgacgg caaaccgtta ctgaccggca aacgttcccc tgccattgaa gagtggctgc
    42241 gcaaggtcaa tggctacgcc aacaaactgc tgttgccgcg ttttgccaaa tcggcatttg
    42301 atgagttttc tactcccgcc gcgcgcaaat atttcgtcga caagaaagag gccagcgcgg
    42361 gtaattttgc cgacctgctg gcccactctg acggtctgat taagaatatc agcgatgatt
    42421 tgcgtgcgct ggacaaactg atcgtcaaac cgaacgccgt gaatggcgaa ctttcggaag
    42481 atgatattca gctatttccg ctactgcgta atctgacgct ggtagccgga atcaactggc
    42541 caagccgcgt tgctgattac cgcgataata tggcgaaaca gacacaaatc aatttgttat
    42601 catcaatggc gatttaatcc tgacccgccg cggcagcctg tattcgcggg ttgccgccgt
    42661 tttcagtttt ttctgctggc gagtttatcg ctggtgacac atgctgcgtt gtgtcaggat
    42721 attgacgcag atttcatgtc gaggactacc atgaacaagt ttttatttgc cgctgcattg
    42781 atcgtcagtg gcctgctcgt tggctgtaat caactcaccc aatacaccat caccgaacaa
    42841 gaaattaacc agtcgcttgc gaaacataat aatttctcaa aagatatcgg tttacccggc
    42901 gtggctgacg cccatattgt tctgacaaac ttgatcagcc aaattggtcg cgaagagccg
    42961 aataaggtta ccctaaccgg agacgccaat ctggacatga actccctgtt cggtagtcag
    43021 aaagcgacca tgaaactgaa gctgaaagcg ctgcccgtgt ttgataaaga aaaaggtgcg
    43081 atcttcctga aagagatgga agtggtcgat gcgacggtac aaccggaaaa aatgcaaacg
    43141 gtgatgcaaa cgttgcttcc ctatttgaac caggcattac gcaattactt taaccagcaa
    43201 cctgcttacg tcctgcgcga agatggcagt cagggcgaag caatggcgaa aaaactggcg
    43261 aaaggcattg aagtgaagcc aggcgaaatt gtcattccgt ttacggatta atgacgaggg
    43321 cgcattcgcg ccctttattt ttcgtgcaaa ggaaaacgtt tccgcttatc ctttgtgtcc
    43381 ggcaaaaaca ttccttcagc cggagcatag agattaatga ctgcaccatc ccaggtatta
    43441 aagatccgcc gcccagacga ctggcacctt cacctccgcg atggcgacat gttaaaaact
    43501 gtcgtgccgt ataccagcga aatttatgga cgggctatcg taatgcccaa tctggctccg
    43561 cccgtgacca cagttgaggc tgccgtggcg tatcgccaac gtattcttga cgccgtacct
    43621 gccgggcacg atttcacccc attgataacc tgttatttaa cagattcgct ggatcctaat
    43681 gagctggagc gcggatttaa tgaaggcgtg ttcaccgctg caaaacttta cccggcaaac
    43741 gcaaccacta actccagcca cggcgtgacg tcaattgacg caatcatgcc ggtacttgag
    43801 cgcatggaaa aaatcggtat gccgctactg gtgcatggtg aagtgacaca tgcagatatc
    43861 gacatttttg atcgtgaagc gcgctttata gaaagcgtga tggaacctct gcgccagcgc
    43921 ctgactgcgc tgaaagtcgt ttttgagcac atcaccacca aagatgctgc cgactatgtc
    43981 cgtgacggaa atgaacggct ggctgccacc atcactccgc agcatctgat gtttaaccgc
    44041 aaccatatgc tggttggtgg cgtgcgtccg cacctgtatt gtttacccat cctcaaacgc
    44101 aatattcacc aacaggcatt gcgtgaactg gtcgccagcg gttttaatcg agtattcctc
    44161 ggtacggatt ctgcgccaca tgcacgtcat cgcaaagaga gcagttgcgg ctgcgcgggc
    44221 tgcttcaacg ccccaaccgc gctgggcagt tacgctaccg tctttgaaga gatgaatgct
    44281 ctgcagcact ttgaagcatt ctgttctgta aacggcccgc agttctatgg gttgccggtc
    44341 aacgacacat tcatcgaact ggtacgtgaa gagcaacagg ttgctgaaag catcgcactg
    44401 actgatgaca ccctggtgcc attcctcgcc ggggaaacgg tacgctggtc cgttaaacaa
    44461 taaaaatcgt agcttcctgt tgtcattagg ttatttaacc tgtataaata accagtatat
    44521 tcaacagggg gctattatgc gaattgaagt caccatagcg aaaacttctc cattgccagc
    44581 tggggctatt gacgccctgg ctggcgaact ttcccgccgt attcagtatg cgtttcctga
    44641 taatgaaggc cacgtatcgg tacgttatgc cgcagcgaat aatttatcgg ttattggcgc
    44701 aacaaaagaa gataaacagc gcattagcga aattctccag gaaacgtggg aaagcgccga
    44761 tgac
//
LOCUS       DAZRUF010000006        44687 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZRUF010000006 DAZRUF010000000
VERSION     DAZRUF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.006"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..716)
                     /locus_tag="ACHXOL_000265"
     CDS             complement(<1..716)
                     /locus_tag="ACHXOL_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR6807352.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHXOL_000266"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHXOL_000266"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR6807353.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAVCAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHXOL_000267"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHXOL_000267"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR6807354.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="ACHXOL_000268"
     CDS             complement(3650..4705)
                     /locus_tag="ACHXOL_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR6807355.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="ACHXOL_000269"
     CDS             complement(4715..5503)
                     /locus_tag="ACHXOL_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR6807356.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="ACHXOL_000270"
     CDS             complement(5521..6573)
                     /locus_tag="ACHXOL_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR6807357.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7446)
                     /locus_tag="ACHXOL_000271"
     CDS             complement(6604..7446)
                     /locus_tag="ACHXOL_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR6807358.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSAGNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7433..8314)
                     /locus_tag="ACHXOL_000272"
     CDS             complement(7433..8314)
                     /locus_tag="ACHXOL_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR6807359.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8335..9645)
                     /locus_tag="ACHXOL_000273"
     CDS             complement(8335..9645)
                     /locus_tag="ACHXOL_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR6807360.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9659..11190)
                     /locus_tag="ACHXOL_000274"
                     /pseudo
     CDS             complement(9659..11190)
                     /locus_tag="ACHXOL_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHXOL_000275"
     CDS             complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHXOL_000275"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR6807361.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHXOL_000276"
     CDS             complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHXOL_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR6807362.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHXOL_000277"
     CDS             complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHXOL_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR6807363.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHXOL_000278"
     CDS             complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHXOL_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR6807364.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHXOL_000279"
     CDS             complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHXOL_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR6807365.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13493..14470
                     /gene="pspF"
                     /locus_tag="ACHXOL_000280"
     CDS             13493..14470
                     /gene="pspF"
                     /locus_tag="ACHXOL_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR6807366.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHXOL_000281"
                     /pseudo
     CDS             complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHXOL_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHXOL_000282"
     CDS             complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHXOL_000282"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR6807367.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHXOL_000283"
     CDS             complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHXOL_000283"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR6807368.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDHAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEEAGLPDGVL
                     NVVSGYGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANI
                     VFADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGH
                     PLDTVTTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNA
                     SLSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSV
                     FVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHXOL_000284"
     CDS             complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHXOL_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR6807369.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHXOL_000285"
     CDS             complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHXOL_000285"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR6807370.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20373..21734
                     /gene="puuA"
                     /locus_tag="ACHXOL_000286"
     CDS             20373..21734
                     /gene="puuA"
                     /locus_tag="ACHXOL_000286"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR6807371.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21883..22047
                     /locus_tag="ACHXOL_000287"
     CDS             21883..22047
                     /locus_tag="ACHXOL_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6807372.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22037..23422
                     /gene="puuP"
                     /locus_tag="ACHXOL_000288"
     CDS             22037..23422
                     /gene="puuP"
                     /locus_tag="ACHXOL_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR6807373.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23556..23801
                     /locus_tag="ACHXOL_000289"
     CDS             23556..23801
                     /locus_tag="ACHXOL_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR6807374.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24114..25757
                     /gene="sapA"
                     /locus_tag="ACHXOL_000290"
     CDS             24114..25757
                     /gene="sapA"
                     /locus_tag="ACHXOL_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR6807375.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25754..26719
                     /gene="sapB"
                     /locus_tag="ACHXOL_000291"
     CDS             25754..26719
                     /gene="sapB"
                     /locus_tag="ACHXOL_000291"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR6807376.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26706..27596
                     /gene="sapC"
                     /locus_tag="ACHXOL_000292"
     CDS             26706..27596
                     /gene="sapC"
                     /locus_tag="ACHXOL_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR6807377.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     MLLVNLLGDGVRRAIIAGVE"
     gene            27596..28588
                     /gene="sapD"
                     /locus_tag="ACHXOL_000293"
     CDS             27596..28588
                     /gene="sapD"
                     /locus_tag="ACHXOL_000293"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR6807378.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28590..29396
                     /gene="sapF"
                     /locus_tag="ACHXOL_000294"
     CDS             28590..29396
                     /gene="sapF"
                     /locus_tag="ACHXOL_000294"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR6807379.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29464..29817
                     /locus_tag="ACHXOL_000295"
     CDS             29464..29817
                     /locus_tag="ACHXOL_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR6807380.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            30185..30973
                     /gene="fabI"
                     /locus_tag="ACHXOL_000296"
     CDS             30185..30973
                     /gene="fabI"
                     /locus_tag="ACHXOL_000296"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR6807381.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31118..32244
                     /locus_tag="ACHXOL_000297"
                     /pseudo
     CDS             31118..32244
                     /locus_tag="ACHXOL_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32312..34246
                     /gene="rnb"
                     /locus_tag="ACHXOL_000298"
     CDS             32312..34246
                     /gene="rnb"
                     /locus_tag="ACHXOL_000298"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR6807382.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34324..34427)
                     /locus_tag="ACHXOL_000299"
                     /pseudo
     CDS             complement(34324..34427)
                     /locus_tag="ACHXOL_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHXOL_000300"
                     /pseudo
     CDS             34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHXOL_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHXOL_000301"
     CDS             complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHXOL_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR6807383.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHXOL_000302"
     CDS             36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHXOL_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR6807384.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36881..37630
                     /locus_tag="ACHXOL_000303"
     CDS             36881..37630
                     /locus_tag="ACHXOL_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR6807385.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37899..38117
                     /gene="osmB"
                     /locus_tag="ACHXOL_000304"
     CDS             37899..38117
                     /gene="osmB"
                     /locus_tag="ACHXOL_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR6807386.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="ACHXOL_000305"
     CDS             complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="ACHXOL_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR6807387.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="ACHXOL_000306"
     CDS             complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="ACHXOL_000306"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR6807388.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="ACHXOL_000307"
     CDS             complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="ACHXOL_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR6807389.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="ACHXOL_000308"
     CDS             complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="ACHXOL_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR6807390.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="ACHXOL_000309"
     CDS             complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="ACHXOL_000309"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR6807391.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            42066..42656
                     /gene="ribA"
                     /locus_tag="ACHXOL_000310"
     CDS             42066..42656
                     /gene="ribA"
                     /locus_tag="ACHXOL_000310"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR6807392.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLNTKAEKMGHLLNK"
     gene            complement(42720..>44687)
                     /gene="acnA"
                     /locus_tag="ACHXOL_000311"
     CDS             complement(42720..>44687)
                     /gene="acnA"
                     /locus_tag="ACHXOL_000311"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR6807393.1"
                     /translation="MLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGV
                     VEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVE
                     LVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKVFAAS
                     NELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKA
                     VILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNSGPLP
                     DPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAS
                     EPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRS
                     DTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPD
                     SPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPD
                     SDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIH
                     RSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEV
                     VPCRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        10538 a        11831 c        11282 g        11036 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac agacagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttaccccttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cgcttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc aacccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtgtcg taaatgccca gcgcggtgat gattttgaac agcggcacca ccagcaaaat
     7081 gccagagaac atatacaccg tgtaaaagct ggcgttgatg gtcatccgac ctttaaagcg
     7141 caggcgggaa agcgcgtaag ccccaagaat gccgaggaat accgccacca cggatgaaac
     7201 cacagacacc accagactgt tacggaagta gtcgacaaac gggaaaatca tcgggttaaa
     7261 aatgtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7321 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7381 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7441 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7501 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7561 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7621 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7681 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7741 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7801 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7861 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7921 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7981 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     8041 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     8101 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     8161 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     8221 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8281 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8341 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8401 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8461 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8521 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8581 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8641 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8701 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8761 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8821 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8881 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8941 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     9001 gttaaagacg ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     9061 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     9121 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     9181 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9241 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9301 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9361 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9421 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9481 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9541 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9601 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9661 atttaatcca cataacctgc caggggcgca gcgttatttc attacccaat agatcaactt
     9721 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9781 cattaaacaa tccagtcaga caattaccat cagcgttact tctttgaata cgcattacgg
     9841 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9901 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9961 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
    10021 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
    10081 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
    10141 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
    10201 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10261 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10321 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10381 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10441 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10501 ggcatccaag cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10561 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10621 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10681 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10741 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10801 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10861 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10921 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10981 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    11041 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    11101 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    11161 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    11221 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11281 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11341 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11401 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11461 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11521 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11581 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11641 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11701 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11761 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11821 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11881 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11941 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    12001 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    12061 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    12121 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccggc
    12181 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12241 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12301 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12361 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12421 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12481 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12541 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12601 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12661 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12721 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12781 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12841 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12901 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12961 aatctctttc ttcatgcgtg ccagcgtatc gtccaccagc gtcacttcat gttccaggga
    13021 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    13081 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    13141 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    13201 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13261 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13321 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13381 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13441 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13501 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13561 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13621 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13681 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13741 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13801 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13861 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13921 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13981 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    14041 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    14101 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    14161 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    14221 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14281 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14341 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14401 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14461 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14521 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14581 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14641 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14701 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14761 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14821 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14881 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14941 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    15001 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    15061 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    15121 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    15181 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15241 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15301 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15361 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15421 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15481 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15541 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15601 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15661 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15721 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15781 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15841 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15901 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15961 cgcccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    16021 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    16081 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    16141 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    16201 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16261 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16321 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16381 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16441 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16501 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16561 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16621 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16681 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16741 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16801 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16861 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16921 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16981 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    17041 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    17101 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    17161 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    17221 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17281 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17341 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17401 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17461 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17521 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17581 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17641 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17701 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17761 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17821 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17881 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17941 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    18001 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    18061 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    18121 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    18181 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18241 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18301 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18361 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18421 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18481 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg
    18541 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18601 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18661 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18721 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18781 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18841 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18901 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18961 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    19021 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    19081 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    19141 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    19201 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19261 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19321 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19381 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19441 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19501 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19561 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19621 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19681 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19741 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19801 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19861 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19921 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19981 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    20041 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    20101 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    20161 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    20221 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20281 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20341 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20401 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20461 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20521 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20581 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20641 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20701 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20761 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20821 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20881 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20941 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    21001 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    21061 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    21121 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    21181 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21241 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21301 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21361 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21421 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21481 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21541 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21601 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21661 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21721 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21781 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21841 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21901 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21961 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    22021 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    22081 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    22141 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    22201 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22261 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22321 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22381 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22441 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22501 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22561 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22621 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22681 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22741 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22801 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22861 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22921 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22981 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    23041 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    23101 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    23161 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    23221 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23281 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23341 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23401 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23461 cgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc
    23521 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23581 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23641 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23701 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23761 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23821 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23881 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23941 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    24001 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    24061 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    24121 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    24181 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24241 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24301 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24361 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24421 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24481 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24541 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24601 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24661 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24721 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24781 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24841 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24901 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24961 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    25021 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    25081 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    25141 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    25201 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25261 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25321 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25381 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
    25441 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25501 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25561 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25621 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25681 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25741 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25801 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25861 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25921 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25981 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    26041 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    26101 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    26161 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    26221 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26281 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26341 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26401 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26461 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26521 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26581 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26641 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26701 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26761 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26821 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26881 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26941 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    27001 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    27061 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    27121 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    27181 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27241 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27301 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27361 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27421 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27481 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcatgttgt
    27541 tggttaacct ccttggtgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27601 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27661 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27721 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27781 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27841 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27901 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27961 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    28021 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    28081 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    28141 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    28201 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28261 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28321 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28381 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28441 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28501 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28561 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28621 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28681 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28741 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28801 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28861 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28921 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28981 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    29041 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    29101 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    29161 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    29221 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29281 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29341 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29401 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29461 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29521 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29581 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29641 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29701 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29761 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29821 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29881 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29941 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    30001 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    30061 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    30121 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    30181 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30241 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30301 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30361 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30421 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30481 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30541 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30601 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30661 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30721 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30781 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30841 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30901 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30961 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    31021 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    31081 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    31141 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    31201 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31261 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31321 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31381 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31441 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31501 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31561 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31621 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31681 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31741 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31801 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31861 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31921 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31981 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    32041 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    32101 gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca
    32161 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    32221 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32281 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32341 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32401 aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca
    32461 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32521 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32581 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32641 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32701 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32761 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32821 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32881 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32941 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    33001 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    33061 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    33121 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    33181 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33241 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33301 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33361 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33421 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33481 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33541 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33601 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33661 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33721 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33781 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33841 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33901 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33961 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    34021 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    34081 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    34141 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    34201 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34261 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34321 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34381 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34441 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34501 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34561 cgatgttttg cgcttttcta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34621 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34681 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34741 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34801 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34861 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34921 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34981 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    35041 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    35101 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    35161 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    35221 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc agataacaat
    35281 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35341 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35401 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35461 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35521 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35581 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35641 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35701 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35761 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35821 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35881 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35941 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    36001 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    36061 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    36121 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    36181 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36241 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36301 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36361 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36421 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36481 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36541 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36601 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36661 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36721 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36781 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36841 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36901 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36961 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    37021 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    37081 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    37141 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    37201 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    37261 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    37321 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    37381 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    37441 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    37501 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    37561 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37621 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37681 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37741 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37801 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37861 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37921 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37981 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    38041 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    38101 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    38161 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    38221 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    38281 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    38341 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat
    38401 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    38461 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    38521 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    38581 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38641 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38701 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38761 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38821 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38881 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38941 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    39001 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    39061 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    39121 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    39181 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    39241 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    39301 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    39361 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    39421 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    39481 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    39541 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39601 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39661 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39721 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39781 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39841 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39901 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39961 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    40021 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    40081 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    40141 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    40201 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    40261 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    40321 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    40381 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    40441 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    40501 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    40561 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40621 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40681 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40741 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40801 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40861 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40921 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40981 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    41041 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    41101 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    41161 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    41221 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    41281 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    41341 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    41401 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    41461 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    41521 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    41581 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41641 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcgaaaa cgcagacacc
    41701 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41761 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41821 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41881 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41941 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    42001 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    42061 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    42121 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    42181 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    42241 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    42301 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    42361 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    42421 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    42481 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    42541 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42601 acgaacatta tctcaatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42661 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42721 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42781 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42841 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42901 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42961 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    43021 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    43081 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    43141 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    43201 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    43261 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    43321 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    43381 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    43441 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    43501 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    43561 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43621 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43681 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43741 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43801 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43861 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43921 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43981 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    44041 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    44101 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    44161 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    44221 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    44281 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    44341 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    44401 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    44461 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    44521 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    44581 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44641 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacat
//
LOCUS       DAZRUF010000007        44173 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZRUF010000007 DAZRUF010000000
VERSION     DAZRUF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44173
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.007"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..560)
                     /locus_tag="ACHXOL_000312"
     CDS             complement(<1..560)
                     /locus_tag="ACHXOL_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR6807394.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(763..999)
                     /gene="feoC"
                     /locus_tag="ACHXOL_000313"
     CDS             complement(763..999)
                     /gene="feoC"
                     /locus_tag="ACHXOL_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR6807395.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(999..3320)
                     /gene="feoB"
                     /locus_tag="ACHXOL_000314"
     CDS             complement(999..3320)
                     /gene="feoB"
                     /locus_tag="ACHXOL_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR6807396.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3337..3564)
                     /gene="feoA"
                     /locus_tag="ACHXOL_000315"
     CDS             complement(3337..3564)
                     /gene="feoA"
                     /locus_tag="ACHXOL_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR6807397.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4002..6323)
                     /locus_tag="ACHXOL_000316"
     CDS             complement(4002..6323)
                     /locus_tag="ACHXOL_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR6807398.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6420..6896)
                     /gene="greB"
                     /locus_tag="ACHXOL_000317"
     CDS             complement(6420..6896)
                     /gene="greB"
                     /locus_tag="ACHXOL_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR6807399.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7124..7843
                     /gene="ompR"
                     /locus_tag="ACHXOL_000318"
     CDS             7124..7843
                     /gene="ompR"
                     /locus_tag="ACHXOL_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR6807400.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7840..9192
                     /gene="envZ"
                     /locus_tag="ACHXOL_000319"
     CDS             7840..9192
                     /gene="envZ"
                     /locus_tag="ACHXOL_000319"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR6807401.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9268..10890)
                     /gene="pckA"
                     /locus_tag="ACHXOL_000320"
     CDS             complement(9268..10890)
                     /gene="pckA"
                     /locus_tag="ACHXOL_000320"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR6807402.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKSLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKLDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11269..12969
                     /locus_tag="ACHXOL_000321"
                     /pseudo
     CDS             11269..12969
                     /locus_tag="ACHXOL_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13104..13982)
                     /gene="hslO"
                     /locus_tag="ACHXOL_000322"
     CDS             complement(13104..13982)
                     /gene="hslO"
                     /locus_tag="ACHXOL_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR6807403.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14007..14408)
                     /gene="hslR"
                     /locus_tag="ACHXOL_000323"
     CDS             complement(14007..14408)
                     /gene="hslR"
                     /locus_tag="ACHXOL_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR6807404.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14419..15087)
                     /gene="yrfG"
                     /locus_tag="ACHXOL_000324"
     CDS             complement(14419..15087)
                     /gene="yrfG"
                     /locus_tag="ACHXOL_000324"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR6807405.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15152..17287)
                     /gene="igaA"
                     /locus_tag="ACHXOL_000325"
     CDS             complement(15152..17287)
                     /gene="igaA"
                     /locus_tag="ACHXOL_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR6807406.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17607..18167
                     /gene="nudE"
                     /locus_tag="ACHXOL_000326"
     CDS             17607..18167
                     /gene="nudE"
                     /locus_tag="ACHXOL_000326"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR6807407.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18332..20884)
                     /gene="mrcA"
                     /locus_tag="ACHXOL_000327"
     CDS             complement(18332..20884)
                     /gene="mrcA"
                     /locus_tag="ACHXOL_000327"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR6807408.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21004..21783
                     /gene="hofM"
                     /locus_tag="ACHXOL_000328"
     CDS             21004..21783
                     /gene="hofM"
                     /locus_tag="ACHXOL_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR6807409.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21783..22322
                     /gene="hofN"
                     /locus_tag="ACHXOL_000329"
     CDS             21783..22322
                     /gene="hofN"
                     /locus_tag="ACHXOL_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR6807410.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTD
                     NAEARVNTVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22306..22746
                     /gene="hofO"
                     /locus_tag="ACHXOL_000330"
     CDS             22306..22746
                     /gene="hofO"
                     /locus_tag="ACHXOL_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR6807411.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22736..23140
                     /gene="hofP"
                     /locus_tag="ACHXOL_000331"
     CDS             22736..23140
                     /gene="hofP"
                     /locus_tag="ACHXOL_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR6807412.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23052..24290
                     /gene="hofQ"
                     /locus_tag="ACHXOL_000332"
     CDS             23052..24290
                     /gene="hofQ"
                     /locus_tag="ACHXOL_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR6807413.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24691..25212
                     /gene="aroK"
                     /locus_tag="ACHXOL_000333"
     CDS             24691..25212
                     /gene="aroK"
                     /locus_tag="ACHXOL_000333"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR6807414.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25269..26357
                     /gene="aroB"
                     /locus_tag="ACHXOL_000334"
     CDS             25269..26357
                     /gene="aroB"
                     /locus_tag="ACHXOL_000334"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR6807415.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26449..27735
                     /gene="damX"
                     /locus_tag="ACHXOL_000335"
     CDS             26449..27735
                     /gene="damX"
                     /locus_tag="ACHXOL_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR6807416.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27842..28678
                     /gene="dam"
                     /locus_tag="ACHXOL_000336"
     CDS             27842..28678
                     /gene="dam"
                     /locus_tag="ACHXOL_000336"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR6807417.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28696..29373
                     /gene="rpe"
                     /locus_tag="ACHXOL_000337"
     CDS             28696..29373
                     /gene="rpe"
                     /locus_tag="ACHXOL_000337"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR6807418.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29366..30124
                     /gene="gph"
                     /locus_tag="ACHXOL_000338"
     CDS             29366..30124
                     /gene="gph"
                     /locus_tag="ACHXOL_000338"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR6807419.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30117..31121
                     /gene="trpS"
                     /locus_tag="ACHXOL_000339"
     CDS             30117..31121
                     /gene="trpS"
                     /locus_tag="ACHXOL_000339"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR6807420.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31281..32186
                     /gene="yhfZ"
                     /locus_tag="ACHXOL_000340"
     CDS             31281..32186
                     /gene="yhfZ"
                     /locus_tag="ACHXOL_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR6807421.1"
                     /translation="MRRTFIKKEGVVIMTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32203..32565
                     /locus_tag="ACHXOL_000341"
     CDS             32203..32565
                     /locus_tag="ACHXOL_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR6807422.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32649..33812
                     /locus_tag="ACHXOL_000342"
                     /pseudo
     CDS             32649..33812
                     /locus_tag="ACHXOL_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33812..35038
                     /locus_tag="ACHXOL_000343"
                     /pseudo
     CDS             33812..35038
                     /locus_tag="ACHXOL_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35035..35913
                     /locus_tag="ACHXOL_000344"
     CDS             35035..35913
                     /locus_tag="ACHXOL_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR6807423.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35924..36277
                     /locus_tag="ACHXOL_000345"
     CDS             35924..36277
                     /locus_tag="ACHXOL_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR6807424.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36289..37593
                     /locus_tag="ACHXOL_000346"
     CDS             36289..37593
                     /locus_tag="ACHXOL_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR6807425.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37605..38690
                     /locus_tag="ACHXOL_000347"
     CDS             37605..38690
                     /locus_tag="ACHXOL_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR6807426.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38842..39573)
                     /gene="frlR"
                     /locus_tag="ACHXOL_000348"
                     /gene_synonym="yhfR"
     CDS             complement(38842..39573)
                     /gene="frlR"
                     /locus_tag="ACHXOL_000348"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR6807427.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39673..40458)
                     /gene="frlD"
                     /locus_tag="ACHXOL_000349"
     CDS             complement(39673..40458)
                     /gene="frlD"
                     /locus_tag="ACHXOL_000349"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR6807428.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40455..41286)
                     /gene="frlC"
                     /locus_tag="ACHXOL_000350"
                     /pseudo
     CDS             complement(40455..41286)
                     /gene="frlC"
                     /locus_tag="ACHXOL_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41336..42358)
                     /gene="frlB"
                     /locus_tag="ACHXOL_000351"
     CDS             complement(41336..42358)
                     /gene="frlB"
                     /locus_tag="ACHXOL_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR6807429.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42379..43716)
                     /gene="frlA"
                     /locus_tag="ACHXOL_000352"
     CDS             complement(42379..43716)
                     /gene="frlA"
                     /locus_tag="ACHXOL_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR6807430.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(44011..>44173)
                     /locus_tag="ACHXOL_000353"
     CDS             complement(44011..>44173)
                     /locus_tag="ACHXOL_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR6807431.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKI
                     IGGCGPTAQ"
BASE COUNT        10303 a        11204 c        12099 g        10567 t
ORIGIN      
        1 tcgcgttgac gaatatgttt ttgaattaac tccagcagcg ccatacgtcg gtgctgcatg
       61 atttcatcat caggcatgac ggtgacatca atcagcggaa atgcggaggc ataaagctgc
      121 cttgccagtt tgggatcggc gaaacaatcc agccaacaca gcgaataggg ataggggctt
      181 tcaataccgt ggtaaaacaa caatagcacc accatcggca acgttttgta tccagcatca
      241 aggtgatttt gcattgcggc aatagcgtaa cgcatcatgc gaaatgcgat cagtttgttt
      301 gaggtgcttt gatgttcaat cagacaatag atgtatcctg gtccttgttc cgttttcacc
      361 gaccacagca catcggaata gctttcacgc agatcgtcat caataaagct gctcgactcc
      421 agcttgaggg ttttcatatc acaaagcgcg tgaatgggtg ccggtaaatg aaacgcaaga
      481 aaatcacgag ccgtgtccgg ttggcgtaaa aagagtttga acagcgcatc gtgtggggtg
      541 gaactctgct ttttgctcat ggcgttcctt cacctgttgt ttgaatgccg cgactctacg
      601 cgccactcca cacctgtgca ttagcgagtt ttcatccctg cgagatgctt tcaaaggaac
      661 ttacgccgtg ctgcaagagt tgcattttta tggaaagccg cacgcaaaaa agcccggatg
      721 cagacgcata ccgggctttc caaaacaggc gatggagtaa ggttaacgca gcgcccacca
      781 ctcgcgcaga caggcttttc cttccgggca gcttttacaa ctgccagaga ggcagccgtc
      841 aggttcttcc tgaatccgca cggctttgcc catactttcc agttgttgca gcatggcgtt
      901 aatcattggc tgcggagtgt tcaatgtctg gcttatctgg gtcgcttcca tacggccccg
      961 taacgccagc aaatcacgta cctgaataag tgaagccatt aatggcaatc accggtggtg
     1021 ctggctgcgc agcaactgct taccgacttg cgggttgcca gcagttcgat atccacccgg
     1081 ctacgcgcgc ggcgcagcag accgataact acgatgttaa acaggattac cgccagaatg
     1141 cacaccaggc tgtaagttgg gtgctgactg tagctggcga cctgatagaa caatgttgcc
     1201 agtgagtaag cgatattcag cccccacagg atggagaagc ccatccagcc acggcttgat
     1261 tcacgggcga tggctcccat caccgagata catggtacat acagcaggac gaaaatcagg
     1321 tagctgtatg cggcagctgc gctaccgaat ttctgatcca tcacgcccat cgccccggta
     1381 cccatttcac cgtcgccttt gctagcttca atggggttca tcagtacgct aaggctgaag
     1441 gtgtctttca ggctctgcca ggtttcatct accgcactga acagctcttc accgaggttg
     1501 aattccgccg gattgaactc ttcgtcctga atattttctg cggtgtagag ggtgttgagc
     1561 gtacccacca ccacttcttt tgccattgcg cccgtaaaca ggccaaccgt tgcctgccag
     1621 ttatcttcat gtacgccaat cggcttgaag acaggggtta tcacccggct gacggacgcc
     1681 agcgccgagt cgttgatgtt atcgacgatt ttcccgctca acgagaagct gttgaaagcg
     1741 ctcaggaaaa tgctgacgat gataatcact ttaccagcac gcagaacgaa gcccttcaga
     1801 cgctgccagg tctggataat caggctttta acgtgtggta catggtagac cggcagctcc
     1861 atgacaaacg gcgtcgcttc accgcgcatg atggtgtact tgagcatcag gccagtcagc
     1921 accgccatca cgataccgag catatacagc gagaaaaccg ccagggcacc gttctgcccg
     1981 aagaaggcag ccgcgaatac tgcgaagata gccagacgcg cgccgcagga cataaacggt
     2041 gccatcatga tggtcatcag acgttcacgc ggtgcatcaa gcgtacgtgc gcccattacc
     2101 gacggtacgt tacaaccgaa accgacgatc agcggcacaa aggatttccc cggcaagccc
     2161 agcgcctgca tcaggcggtc catcacaaac gctgcacgcg ccatatagcc ggagtcctca
     2221 aggaaggaga ggaacaggta catcatgccg atctgcggca ccagtggcag cacggtgtta
     2281 ataccgccac ccaggccctg ggcgaggaag atagtcagcc agtccgggaa gtggagcgtg
     2341 tagccaatcc attgaatacc atgcacaaat agcgccacgg agccgacgtc aaacagcggc
     2401 tgtaacgccc cgccgatgtt gatagccagc aggaacatca ggtacatcac aaagaggaaa
     2461 atcggcagac cgaggaaacg gttgagcacg attttatcta ccgcagtggt gaaacggctg
     2521 ggttctgccg tcagggtgtt gcttaccaca tcacagatgg cagctatgca ctggtaacgc
     2581 gcatcggcaa tgtgcagcgc cggatcatcc atttcattac gcagacgggc aagggcggca
     2641 tccagatgct gcgacgcttc accggcgtag gcgcggctgt agatatcgcc ttccagcatt
     2701 tgcaggccca gccagcgacg ttgtttcagc gggatgtcgg aaggcatcac ttttgccagt
     2761 gaatctgctt cgttgagtag cggctgtgcg taatgcacca gttccacatt ctcgttagct
     2821 ttatagcgat caatcgccag cttgagcgct tcaataccgc gaccacgggt tgaaaccagc
     2881 gggatcactg ggcagccaag acgcgccgac agagcatcaa tttcaatacg aatattttgc
     2941 ttctcggcaa tgtcgagcat gttcagtgcc acgatgcagg gaatgccgag ttccagcagt
     3001 tgtagcgtca ggtacaggtt acgctcaagg ttagacgcat ccaccacgtt aatcagcagg
     3061 tcggcgtcgc cactcaaaat gtagtgacag gcgatttgct catcgagcga ggtctgcgat
     3121 gagatggtgg tcagagaata ggtgccgggc aggtccacca gcgtgacctg atgatcggtg
     3181 gtggagaatt gcccttcttt acgttcgacg gtaacgccag cccagttacc tacacgctga
     3241 cgtgcgctag tgagctggtt aaataaggtt gtcttgccag aatttggatt accaattaag
     3301 ccaatggtta attttttcat tgttgttatc accgtattaa caggaaaccg cttccacttc
     3361 taataaggcc agatcttttt tgcgtaatac caggctcaca cgacgggttt cgatatgaat
     3421 ggggtcgccg agtggagcga cgcgcaccac attaaaagag gagccaggta acatgccaag
     3481 agaaagcagt ttttggcgat atgccgggct gatttcacgg gaaaagccag tgattttcca
     3541 cgcagtatct ggagtgtatt gcataggtgc ctacttgttt ctcattaact ggataactac
     3601 ctcatggggc gtttgacagc gacatgagac cagatgattt gccaacaaag gagagattaa
     3661 ataatcaaca acacaatgat aatgagaatg gtttctatca gcaatactta aaatgtgtgc
     3721 gaatgttggt ttgaataata atctgactca atgttgatgt ggctcaaggt tatatcgcag
     3781 cgaaaatata attcgaatag tgaattaccc ggactggcta atgtttaatt aaggtttcag
     3841 ttgttaatga agtgataaat attgccggaa tatattatga gtttggttgt ggaataaaaa
     3901 ggccggagat attctccggc acttttatgt caggcaaggc gttcgttgcc agatgcggcg
     3961 tgaacgcctt atcgcgcagg tttaggggcc agtccgggcg tttaacgttt tttgcccatt
     4021 gccgccgcca gcgcatccat catcgcgcta ttaccggcag gctgcgcttc acgaccgcgt
     4081 ggtttggctg ccgggcggtt gttttgcggg cgttcattac cgccgccgcg acgggcgttg
     4141 gtttcgccag gctgctcgtc cagacgcatg gtcagggcga tacgtttacg ctgaagatcc
     4201 acttccagca ctttcacctt cacaatgtcg cccgctttca ccacggtatg cggatcttcc
     4261 acaaacttgt tcgacaatga agagatgtga accaggccgt cctgatgcac gccaatatcg
     4321 acaaacgcgc caaagttggt gacgttggtg acagcgcctt cgaggatcat acccggttgc
     4381 aggtcgttca ttgtctcgac gccatcggca aactgagcgg ttttaaattc cggacgcgga
     4441 tcgcgacccg gtttttccag ctctttgatg atgtcagtta ctgtcggcac accgaatttt
     4501 tcatcagtaa agtcagacgc tttcaggtta cgcagttcgc tgctgttacc catcagatct
     4561 ttcagtgcct gctgtgttgc tgccagaatg cgttccacca ccggataggc ttccgggtga
     4621 acggtagagg cgtccagcgg gttatcaccg tggttaatgc gcaggaagcc cgcgcactgc
     4681 tcgaaggctt tcggccccag acggctgact ttcagcagtt gctgacggtt cttgaactgg
     4741 ccgttctcat cgcgccaggc cacgatgttt tgcgccatca tgcgcgtcag gcccgccacg
     4801 cgagttaata gcggaacaga agcggtgttg agatcgacgc caacggcgtt tacgcagtct
     4861 tctactactg catccagttt gcgggccagt tgcgtctggc tgacgtcatg ctgatactga
     4921 cctacgccga tagatttcgg atcgattttc accagctccg ccagcggatc ctgcaaacgg
     4981 cgggcgatag ataccgcacc acgcagcgaa acgtcgagat ccgggaactc ctgcgctgcc
     5041 agctcggaag ctgagtaaac cgacgcgcca gcttcgctga cgatcacttt ctgtgcggtc
     5101 actttcggga actgcttctg cacgtcgaga tagaaacgtt cagtttcgcg ggaagctgta
     5161 ccgttgccga tcgccaccag ttcaacgtta tgtttttcac acaaggcagc aacggtcatc
     5221 gctacttttg cggcttgtcc ggtgtgcggg taaatggtat cggtcgccac cagtttgcca
     5281 gtggcatcga ccactgccac ttttaccccg gtacgcagac ccggatcgag gcccatcgtt
     5341 gcacgcagtc cggcaggggc cgccatcagc agatcgtgca ggttacgggc aaagacgttg
     5401 attgcttcat cttccgcgcg ttcgcgcacg gtacccatca gttcggtttc cagatgcatc
     5461 agcaccttga tgcgccaggt ccagctcact acgcctttgc gccagctatc cgccggggca
     5521 ttgttcaggc gcaggccaag gtgatccatg atgatttgct cgcaatagct ctctttgggc
     5581 ggctcatcga actgcggatc ggcattcagc gaaagctgga gtacgccttc gttacgccca
     5641 cggaacatcg ccagcgcgcg gtgagaaggc accgtggaca acggttcgtg atgatcgaaa
     5701 tagtcgcgga atttcgcccc ttcctcttct ttaccgctca ccaccgtaga aaccaaatgc
     5761 gcgttcttcc acagataatc acgcactttc gccagcagcg cggcatcttc ggcaaaccgt
     5821 tccatcagga tatagcgcgc gccgtccagc gcggctttgg tatctgccac gcctttatcg
     5881 gcatcaatat attgcgcagc ggcgacttct ggtgtgtgtg acggatcgct ccacagcagg
     5941 tcagccaacg gctcaagccc tgcttcaatg gcgatttgcc cgcgggtgcg gcgtttaggt
     6001 ttgtagggca ggtagaggtc ttcgagttcg gttttgctta gggtggcgtt gatggccttc
     6061 gccagatcat cggtgagttt gccttgctcg gaaatggact tgaggatcgc ctgacgtctc
     6121 tcttccagct cgcgcagata gctcagacgc gtttccagat tacgcagctg cgtgtcatcc
     6181 agaccgccgg tgatttcctt acgataacgt gcgataaacg gcacggtatt cccttcgtca
     6241 agcaggcgaa cggcagcgtc aacctgttcc gggcgcgcct gaatttcacc cgcaataatg
     6301 cggcagaacg aatcattcat catggtttga gttcatcttt tcggatcaaa aatcagggga
     6361 tagttatacg gactggctgg caaaaatgcc agccatcggc aggaggttaa gactcttcat
     6421 tacggtttca cgtattcgat agcattaaca taccagctcg cttccccggc aggggtattc
     6481 accaccgcca gatcgccgac ttcttttttc agcaatgcgc gggccatcgg ggaatcgata
     6541 gagatgtaat ctttacggcc aaaaatttca tcgtagccga caatacggaa acggtgagtc
     6601 acgccatcgt cgttttcaat ctccacccac gcgccaaaaa agactttgcc ttcctgctga
     6661 ggggagtaat tgacgatttt gagattttcc atgcatttag tgagatagcg cacgcgacgg
     6721 tcgatttcac gcagacgctt tttattatac tgatagtcag cattttcgct gcggtcgccc
     6781 agacttgcgg cccaggtcac cttttttgtg acctccgggc gttcttcacg ccagagataa
     6841 ttaagctctt gtttgagttt ttcataccct tcccgggtaa ccaggggcgt tttcatctcg
     6901 ttgattccct ttgtctgttt gataatgcgc acattgggta taacgtgatc atatcaacag
     6961 aatcaataat gtttcgccga ataaattgta tacttaagct gctgtttaat atgctttgta
     7021 acaatttagg ctgaaattca taccagattt agctggtgac gaacgtgagc ttttttaaga
     7081 atacacgctt acaaattgtt gcgaaccttt gggagtacaa acaatgcaag agaactacaa
     7141 gattctggtg gtcgatgacg acatgcgcct gcgtgcgctg ctggaacgtt atctcaccga
     7201 acaaggcttc caggttcgaa gcgtcgctaa tgcagaacag atggatcgcc tgctgactcg
     7261 tgaatctttc catcttatgg tactggattt aatgttacct ggtgaagatg gcttgtcgat
     7321 ttgccgacgt cttcgtagtc agagcaaccc gatgccgatc attatggtga cggcgaaagg
     7381 ggaagaagtg gaccgtatcg taggcctgga gattggcgct gacgactaca ttccaaaacc
     7441 gtttaacccg cgtgaactgc tggcccgtat tcgtgcggtg ctgcgtcgtc aggcgaacga
     7501 actgccaggc gcaccgtcac aggaagaggc ggtaattgct ttcggtaagt tcaaacttaa
     7561 cctcggtacg cgcgaaatgt tccgcgaaga tgagccgatg ccgctcacca gcggtgagtt
     7621 tgcggtactg aaggcactgg tcagccatcc gcgtgagccg ctctcccgcg ataagctgat
     7681 gaaccttgcc cgtggtcgtg aatattccgc aatggaacgc tccatcgacg tgcagatttc
     7741 gcgtctgcgc cgcatggtag aagaagatcc agcgcatccg cgttacattc agaccgtttg
     7801 gggcctgggc tacgtctttg taccggacgg ctctaaagca tgaggcgatt gcgcttctcg
     7861 ccacgaagtt catttgcccg tacgttattg ctcatcgtca ccttgctgtt cgccagcctg
     7921 gtgacgactt atctggtggt gctgaacttc gcgattttgc cgagcctcca gcagtttaat
     7981 aaagtcctcg cgtacgaagt gcgtatgttg atgaccgaca aactgcaact ggaggacggc
     8041 acgcagttgg ttgtgcctcc cgctttccgt cgggagatct accgtgagct ggggatctct
     8101 ctctactcca acgaggctgc cgaagaggca ggtctgcgtt gggcgcaaca ctatgaattc
     8161 ttaagccatc agatggcaca gcaactgggc ggcccgacgg aagtgcgcgt tgaggtcaac
     8221 aaaagttcgc ctgtcgtctg gctgaaaacc tggctgtcgc ccaatatctg ggtacgcgtg
     8281 ccgctgaccg aaattcatca gggcgatttc tctccgctgt tccgctatac gctggcgatt
     8341 atgctattgg cgataggcgg ggcgtggctg tttattcgta tccagaaccg accgttggtc
     8401 gatctcgaac atgctgcttt gcaggtcggt aaagggatta ttccgccgcc gctgcgtgag
     8461 tatggcgcgt ccgaggtgcg ttccgttacc cgcgccttta accatatggc ggctggtgtt
     8521 aagcaactgg cggatgaccg cacgctgctg atggcggggg taagtcacga cttgcgcacg
     8581 ccgctgacgc gtattcgcct ggcgactgag atgatgagcg agcaggatgg ctatctggca
     8641 gaatcgatca ataaagatat cgaagagtgc aacgccatca ttgagcagtt tatcgactac
     8701 ctgcgcaccg ggcaggagat gccgatggaa atggcggatc ttaatgcagt gctcggtgag
     8761 gtgattgctg ccgaaagtgg ctatgagcgg gaaattgaaa ccgcgcttta ccccggcagc
     8821 attgaagtga aaatgcaccc gctgtcgatc aaacgcgcgg tggcgaatat ggtggtcaac
     8881 gccgctcgtt atggcaatgg ctggatcaaa gtcagcagcg gaacggagcc gaatcgcgcc
     8941 tggttccagg tggaagatga cggtccggga attgcgccgg aacaacgtaa gcacctgttc
     9001 cagccgtttg tccgcggcga cagtgcgcgc accattagcg gcacgggatt agggctggca
     9061 attgtgcagc gtatcgtgga taaccataac gggatgctgg agcttggcac cagcgagcgg
     9121 ggcgggcttt ccattcgcgc ctggctgcca gtgccggtaa cgcgggcgca gggcacgaca
     9181 aaagaagggt aaataaacgg gaggcgaagg tgcctcccgt tttgctttct ataagatact
     9241 ggatagatat tctccagctt caaatcatta cagtttcgga ccagccgcta ccagcgcggc
     9301 acccgcaggg gtgtcggtgt atttatcgaa gttgtcgata aacagtttcg ccagggtttc
     9361 ggctttttcc tgccactgtt ccggagaagc gtaggtgtta cgcggatcga gaatcttcgt
     9421 gtctacgccc ggcagttcgg ttgggatcgc caggttaaac atcggcagag tgaaggtttc
     9481 tgcattatcc agcgaaccgt tgaggatggc gtcgataatg gcgcgggtat ctttaatcga
     9541 gatacgtttg ccagtgccgt tccagccagt gttaaccaga taagcctgcg cgcccgccgc
     9601 ctgcatacgt ttcaccagca cttctgcgta ctgagtcggg tgcagcgaca ggaacgccgc
     9661 gccgaagcaa gcggagaagg ttggcgtcgg ttcggtgatg ccacgctcag taccggccag
     9721 tttggcggtg aagccagaga ggaagtgata ctgggtttga tcggcagtca ggcgagaaac
     9781 cggcggcaac acgccgaaag catcagcagt caggaagata accttagtcg cgtggcccgc
     9841 tttggaaacc ggcttaacga tgttatcgat gtgatagatt ggataagaaa cgcgggtgtt
     9901 ctcggttttt gaaccatcat caaagtcgat agtgccatct tcacgcacgg tgacgttttc
     9961 cagcagcgca tcacgacgga tagcgttgta gatttcaggt tccgcttctt tcgacagctt
    10021 gatagttttt gcgtagcagc cgccttcgaa attaaacacg ccgtcatcgt cccagccgtg
    10081 ttcgtcatcg ccaatcaggc ggcgtttcgg gtcggtggaa agggtggttt taccggtgcc
    10141 ggaaaggccg aagaacaccg caacatcgcc tttctcgcca acgttggcgg agcagtgcat
    10201 agaagcgata cctttcagcg gcagcaggta gttcatcatc gagaacatcc ctttcttcat
    10261 ttcgccgccg taccaggtgc cgccaatcag ctgcatgcgc tcggtcaggt taaacgccac
    10321 gaagttttcg gagttcagac cctgttcttt ccactgcggg ttagtgcact tcgcgccgtt
    10381 cataacgata aagtctagtt tgaaacctgc cagctcttca tcgctcgggc gaataaacat
    10441 gtttttgaca aaatgcgcct gccaggccac ttcggtgatg aaacggacgg aaagacgagt
    10501 atccgggttc gcaccacaga aagcgtcgac aacgaacaga cgtttgccgg aaagctgcct
    10561 ggtcaccagg cttttcagat gctgccaggt ttccggagag agaggtttgt tgtcgttctt
    10621 acctttgcct ttgtctgccc accagaaagt atcgcgagtg gtatcgtcac ggacgatata
    10681 cttatctttt ggtgaacgac cggtgaagat cccggtatcg acggcaacgg cacccagatt
    10741 agttaacacc ccgcgctcat aacctgtcag gctcggatcg agctcttcct gatacagcag
    10801 gtcgtagctt gggttgtaaa cgatatcatg tacgtcactg ataccataag cctcgagttc
    10861 ttgcggggtc aaaccattgt taacgcgcat ttcactgctc cttagccaat atgtattgcc
    10921 tgaatagtaa agtctttttg ggggtgttaa ccgcgacaag gctcatagat ttacgtatct
    10981 ggagaaattc atatttgaca aaaaactccg tgattcctgt cacgaaacgg ttgctattat
    11041 cgcagaaata gcattttagg tgtggaaaat gttctcaaaa ggttaaatct tggtttatat
    11101 ggcgggaata ttgtgagtgg aatcgcattc ctgaatgaaa ttgatgcaaa ttcaaccggt
    11161 aaagtatcga ttcaccacgc taactttttt tgattatttt ctgaaaaaag caaaatacta
    11221 ctgcccggat gcgataatta atccaccttt tgattctgga taaccaacat ggataacgtt
    11281 gaactttcac cggcaacacg ctgggggatg attgctaccg gattacttca ggggctggtt
    11341 tgctacctgc ttatcgcctg gcttgccgga aaaaatcaca gttggattgt ttatggcgtg
    11401 cctgcgaccg tcgcgttttc atccgtttta ctcttctccg tgatctcttt taaacagaag
    11461 cgcctttggg gatggctggc gctggtgttt attgccacga cagggatgag cggttggctg
    11521 aagtggcaaa ctgatggcat gaacccctgg agagctgaaa aggcgatttg ggattttggc
    11581 tgatatctgc tgttgatggc aatgttgttg ctaccgtgga tacaacaaag ccttcgtatc
    11641 cgcaatggca gtagccgcta tagctacttt taccagtcag tatggcataa cgtacttata
    11701 ttactggtga tttttctctc caatggcctg acgtggctgg tgcttttact gtggggtgag
    11761 ttgtttaaac ttgttggcat cacgtttttc aatacgctct tttttgcaac cgactggttt
    11821 atgtatctta catttggtct ggttaccgca ctggtggtga tcctcgcgcg tacacagtca
    11881 cgtttaatcg actctattca aaagttgttc acgctaatcg ccacggggtt gctgccgtta
    11941 gtatcattgc taaccctgat gtttatcatc accctgccgt ttacggacct gagcgcgatt
    12001 tctcgccaca tctccgccgc cgggttgctg ttaacgctgg cctttttgca attgatctta
    12061 atggctattg tccgcgatcc gcaaaaagcg tcacttccct ggacggggcc gttgcgttgc
    12121 ctgattaaaa ccgctttgct ggttgctccg ctgtatgtgt tcgtcgccgc ctgggcgtta
    12181 tggctgcggg tcgctcagta cggctggact gtcgaccgct tgcagggcgc gctggcggtg
    12241 ctggtgttac tggtgtggtc gctggggtat tttgtcagta tcgtctggcg taatgggcaa
    12301 aatccccttg ttcttcaggg caaagtgaac cttgcggttt cgttattggt gttggtgata
    12361 ctggtgcttc ttaattcgcc ggtgctggac agtatgcgca ttagcgtgaa cagtcatatg
    12421 gcgcgttatc agagcggcaa aaacacgcca gaccaggtaa ctatctacat gctcgaacag
    12481 agcggtcgct atggacgtgc ggcgcttgag tcgctgaaaa gcgatgccgg gtttatgaaa
    12541 gacccgaaac gcgcgcggga tctgctgatg gcgttagatg gagagcaaca tcttcaggaa
    12601 caggtatcgg aaaaagtatt agccgaaaat gtgttaattg ctcctggttc tgttaaaccg
    12661 gatgcgacat tctggtcggc cttaatccag gatcgctata acgtgatgac ctgtattgaa
    12721 aaagacgcct gcgtcctggt cgagcaagat ctgaatagtg atggtcaggc ggagcggatc
    12781 ctgtttgctt ttaatgatga cagagtcatt gtctatggct ttgactcaga cagaaaagaa
    12841 tgggacgcgc ttgatatgag tttacttccg aacgaaataa cgaaagaaaa attactcaca
    12901 gctgcgaagg atggaaaact ggatctgacg gtagatggtg aaacgctgga aataaactta
    12961 agtaagtgac aactctttta acggatacga cgctgtatct gaccaacaat aatttgcgat
    13021 ttatttcaca ggcctggtaa gcgcagcaca tcaggccatt acgtcgtaaa atccggcaga
    13081 gccctctctg ccggacatac tcattaatga acttgcggat ctgccggaga cgcgttgttg
    13141 cggatttcag caatatccat cgcattgaac agatagtggt taccgcagta atcacaatgc
    13201 atgtcaattt cgccatcttc cgccaggatg ctatcgactt cttcatcagg cagcgttttc
    13261 agcgcgtcgg cgcaacgttc acgcgagcag gtgcatttga actccacatc ctgcggatcg
    13321 taaaccgtca cctcttcttc gtgatacaaa cgccacaaca cttcgtttgc cggtaaggtc
    13381 agcagttctt cggttttgat ggtttcggtt agcgtcgcca ggtggtcaaa gtcgtcctgc
    13441 tgggcatttt gcgcaggcat tacctgcaac aacataccgc ctgcagccgg tttgccgtct
    13501 acgtcgccgg tgcgaataaa caggcgcgtc ggcagctgtt cagaacgcat aaagtaatct
    13561 tccaggcagg ccgccagggt atcaccttcc agaccaacca cgccctgata gcgttcgcct
    13621 tcgctcgggg taatggtgat caccacgtaa ccattgccga ccagcgtttt caggtcggca
    13681 ttttctggaa tttcgccctg cacgcgcgcc acaccgcgca tttgctggtt attgttaccg
    13741 ttaataaccg ccagattcat cggaccgtcg ccttgcagct gtacggtgat atcaccatca
    13801 aacttcagcg tagcggttaa caggctggtc gcaaccagca gttctgccag cacgttttta
    13861 acgggctgcg gataatcgtg attctcaagg atctgttgca gggtttccga aacggttacc
    13921 agttcgccgc gcacggcaaa gttttcaaac agatagcgat gtaattggtc atgttgcggc
    13981 ataatcatct ctcttgcagg tgacagttat tcactgtcgc cgtgtttaaa tcgtaacagg
    14041 tcgcggcgct cttttttgtc cgggcgtcgg tccgggtgcg gcatggttaa ggcattaagt
    14101 ttacgtgcca gcgccatttt ttcgcgtttc tctacacttt ccgcagtctc ttcatacagc
    14161 aaggctgcct cgctggcggg gcgacgctgt tcagtaatcg cctttacaat caccgtgcgt
    14221 tcgtcatttc cctggcgcag agtgagcgtg gcattcagct cgacgatttt gctcggcttg
    14281 ctgcgctgcc cgttgtaatg caccttaccg ccttcaatca tttcacgggc cagcgcgcgg
    14341 gttttataaa aacgggcagc ccatagccat ttatccagtc gaacctcaac agcaggtttt
    14401 tctttcatgg cgtctccttc acattagcga ggggatcagg cggcggtagt cattcagtga
    14461 cggatggcgt tgatactgtt tctcggcaat cccggaatca ggattagtca cgccgaggca
    14521 gtaacgaata ccaaattgcg cggcagcatc gagaatcgct tcgctgtcat caataaacag
    14581 cgttctttca gctttcagac ccgtagcttc ggccaccgca tgccataacc gctgatcctc
    14641 tttcggataa ccaaatgtgt gggtggaaag taataaatca aggtgtgcgt ccagaccggt
    14701 atgctcaagt tttaccgcca ggttgtgcgg atgcgcattg gtgagcaaaa ttcgctgctt
    14761 accgctggct ttcagtgcct caagaaacgg aatggtatct tcacgcagta cggcacgcgg
    14821 tcccatctcg gtggtcatcg cacagatatc cagacccagt tgctcactcc agtaatcaag
    14881 acagtaccag tttagcgtat gctgtacgtc gtgatattgc tggcgcatat attccatcgc
    14941 ttcctgtggc gtaaccccgt ttttcgcgcc ccatgtttca ggcaccagct tttgccagaa
    15001 atagttatcg aaggcgaggt cgagcaacgt gccgtccata tccagcagaa cggtatctac
    15061 gtcctgccag gcaatgttga tatgcatgag ggaaatctcc agagtgaagc aatttgcgcg
    15121 acagggtagc ataacctgcc gcgcaaacgt gttattcgat aaggctttct gaaggggtga
    15181 tcagttgcgg gttcaggcag ctttcataat aggcctgaat cttcatcatg cgagtgcgat
    15241 gacgctggta acgacgccag gcctgtacgc cattgtaaat ggcgctaccc agcatcgtca
    15301 gtagcagcaa tgtggtgctg agatagcgcc acaggccgga acgatccggg attggatgaa
    15361 ggccaatatg ctgcgtacca ttggcgtcag tgaagatttt tgtgacgata ccctcggcgt
    15421 taaacggcgt atgcatcagc atttgtgcca gtttctggaa agcgttccac tgttcttgcg
    15481 gcgggtagtc gtaaagtgat gccgaaggcc agggctgatc aacaaaatcg ctgccttcgt
    15541 cgctgacaat caggaatccg ccgggggccg gactgtttag tgattgtgcc gctcgcgccg
    15601 tttcatgcgt gataaacggc gcggtggagg ttgccaccag gttatccagc gattccgcac
    15661 tcaccgggcg taataacaca ttcacgccat cgagcttccc ggcgttggcg cgttttacca
    15721 gcgcgtccca gtctttactg ttgccgagat tgaccagcgc atttttcagt cgcacacagt
    15781 catctttggc agaacataaa tccgctgtct tcagtacaat gtcgccaaaa tcatcaagca
    15841 ataccatgcc ggatttttga attgctgaac gtaatgaggc actgacgcga gatccatctt
    15901 ccggtttagg gtgcagctgg cgattaactg cttcagtcaa tgccgtcgct ttgttgacca
    15961 gttcagattc tggtaatggc aatgagcggg cgtcgttcca gatgatctgc gagcagtcaa
    16021 acggtaaaaa aggtgaattg gttttcgcgc tccaggttcc ggaagtgcga atattacaca
    16081 ttcccgtacc gctgatacgc aatgtatcgc ctacccgcac gccagcgtca gccagttgtt
    16141 ttacgctggt ggcttcaatg gtctgtgcgc ctttcatcca tgagagagtg aatttcagcg
    16201 gcatatccag cgggatccag aataacagca taaacagcac cagcagcgag cccgcagcga
    16261 taatcgtact gcgcagccag tgctgtaacg gaaagttttt tacttcatca tgcagcgaaa
    16321 gatatcgccc ctgacgcact acgtggcggt cgagatagat atcaatatcg gtttgttgac
    16381 cgagatcctg agcaatgtat ggctgccagt gtgcgggata gaccaggtcg ataataccga
    16441 gtgaaatatt gttgatctgt tcctgatcgt tttcgccaaa caatccccaa cgacggggtg
    16501 taccgcgcag acaatgaatt tcccgcaggg aggattttgc cgggggcgcg aatagccccc
    16561 acagacctgc gcccagcagc agtaatgcgc cgcctgccag ccacggaaca aatacatccg
    16621 gggtaatcag gcagaaaaag aacatcagga aggaggcgac gatcagcaac gcttcacgca
    16681 gcccgcgcgg acgactcagc gcatattctt catgcgtttc tttgcgaata ttgagtagct
    16741 cgatttgctc actctcttcc ccgcgaatcg acgcctgcgt ggacggaaca ggttgcaagg
    16801 catagccgcg cgtttcctgc atgtactcct gcagcgtatg accgttgagt gaaataacca
    16861 gcggcagcga atcggtataa atcagttcaa cggtattctc atcgttgatg tattgttccc
    16921 agaaaggggg caggtggact tctaccgaat cgaggtagta acgccattta ttcggatcgt
    16981 cggtagagat gccgtaacgc gtgatagcgt gtgtcagcat catgacgttg ttgctttcgg
    17041 cattcagcgc cagagagatc ggtgccgcgc tagctcccgt tgggccaggc acctgtaata
    17101 cctgcgtcag gctctcaaga taattttcaa cggcgctacg ttcttcaggt gtgagtttac
    17161 gcgtttgcgc atccgcgaag gcgttggtcc agggcagctg acgccgtctg gatcgcactt
    17221 ttatcagcca tcccgcaagt agtgagcagg ccagcaaagc agctaaaaaa atcacaatgg
    17281 tgctcatgct ttccccatct tacttagtct gtcaggcgtg gtcagtcgca ccagacacaa
    17341 tacatgattc tgcggttggc tatcggcaag cgtggagttt aacttgagca tcattaagca
    17401 catcacagta accgctgatc atagcaaacc ctctgaaggc gggatatcag caaattcctg
    17461 gagttatttc aacctattga cttttaattt gatttgagga caattattta ttaagttaca
    17521 taaatgtaaa taacatgcaa ttcacattgc cgaaagcgtg tccgatatcg cacaataacc
    17581 gctgatttca cagcatagat cccacgatga gcaaatcatt acaaaaaccc accattctga
    17641 atgttgaaac tgtagcccat tcccgactgt ttaccgtcga gagcgtggat ctggagttca
    17701 gcaatggcgt gcggcgtgtt tatgaacgaa tgcgtccaac caaccgggaa gcagtgatga
    17761 ttgtgccgat tgtggacgat cacctgatcc tgatccgcga atacgcagtg ggaactgaat
    17821 cctacgaatt aggtttttcg aaaggattaa ttgatccggg tgaaagcgtc tacgaagccg
    17881 ctaaccgcga gctaaaagaa gaggttggat ttggtgcgaa cgatctgact tttttgaaga
    17941 agctcagcat ggcaccgtct tacttttcca gcaaaatgaa tatcgtggtc gcgcaagatc
    18001 tctacccgga atcgctggaa ggcgatgagc cagagccgct accacaggtg cgctggccgc
    18061 tggcgcatat gatggatttg ctggaagacc ctgacttcaa cgaagcgcgt aatgtcagcg
    18121 cgctgttcct cgtgcgcgaa tggttgaaag ggcaggggcg agtgtaaatg cgtgaagttt
    18181 gcctgatgca caacgattat caggcttatg gggttcctgc aatatattga tttttcacga
    18241 ttttgttagc cggataacgc gttcacccgc atccggcata aacaaatgca ctttgtcagc
    18301 caactgaaaa ggcgccgaag cgccttttta atcagaacaa ttcctgtgcc tcaccattat
    18361 cgataatggt tgttcccacc tcgtgcaccg cctgttgtgt cggctgcgta ccttcgatga
    18421 aatactcttc gcggctgttg ccaccattag ctaactgccc ggtgctgcga tcgatattca
    18481 ccgtcacaat acccggaggc ggcgtcagcg gctgctccgg cacaccttca agaacggctt
    18541 tcatataagc gtcccatgca ggctgggcac tcttggcacc gccttcgtaa cctgagatct
    18601 gatctttaat cgctccggaa gccgttgtat gaccgagatt acgacggtga tcatcaaagc
    18661 caatccagac cgaggtcaca acgcccggac cgtaacccga gaaccacgca tctttcgaac
    18721 tgttagtggt accggttttc ccgccgatat cgcgacgctg caaatcacga cctgcacgcc
    18781 agccggtacc ctgccagcct ggttcaccaa agatattggt gttcaaagca ctcttaatca
    18841 ggaatgccag cggagtgttg atgacgtgcg gtgcgtactc ctgcgcgcca gtcttcgcca
    18901 ctaacgcctg atttgcctgc tccagctgcg gcattggtac agaaacattc tgctgctcgc
    18961 gggagatagc gacatcttca acatcgttat tttccagcac gttcgatttc tgcgtatcac
    19021 cgtaaatcac cggaatatcg cattccgggc aggctacttt cggtttcgct tcgaaaatca
    19081 cgccgccctg atcgttttca attttgctga taaaccacgg gtccaccagg aagccgccgt
    19141 tcgccatgac cgcgtagccg cgcgccacct gcattggggt gaaggacgct gaacccagcg
    19201 ccagcgattc ggtgtggaca atgttttgcg ccgggaagcc gaagcgttgc agatattctg
    19261 cagcgtagtc gacgcccatc gcccgcattg cacgtaccat caccacgttt ttcgactgac
    19321 ccagcccctg acgtaagcga attggaccag catactgcgg tggtgagttc ttcggctgcc
    19381 agtcagaacc gacacctgca tcccagcgag aaattggcac atcgttcaac atacttgcca
    19441 gcgtcagacc tttatccatc gccgcggtgt agaggaacgg tttgatgttg gaacccacct
    19501 gacgcagtgc ctgggtggcg cggttaaact tgctctgatt gaaatcaaag ccaccgacca
    19561 gcgccataac ggcaccattt tgcggattga tcgacaccag cgccgagttc acttccggca
    19621 cttgtgccag ccaccatgca tcgccaacct gacgaaccca gatttgctga cccgtttgca
    19681 gaacatcggt cactttacgc ggcgtcggtc cctgctgagt atccgaacgg taaggacgcg
    19741 cccagcgaac gccgtccata ctcaatgcga cagtcgaccc gtccgccagc attgccgtcg
    19801 cttcctgagg attggcgctg gtgactgcgg caggcagcag cggaccatag gtcggcagcg
    19861 ccttcagcgt atcggtaatc ttgttgttat cccacgccga ctcgcccact ttccacagca
    19921 catttgccgg gccgcgatag ccgtggcgca tgtcgtagtc cagcacgtta ttacgtaccg
    19981 cctgctgtgc ggcctgctgc actttgcggg tgatggtggt gtaaatgcga taaccgtctt
    20041 cataggcact ttcgccataa cggttataca tctcctggcg caccatttcg ctcaggtacg
    20101 gcgcagagaa agcaatctcc ggcgcgtgat agttagcgtt aatcgcctca gtgcgtgtct
    20161 gatcgaactg ttgttgggtg atataccctt catccagcat ccgcgacagt acgacgttac
    20221 gccgcgcgac ggcacgatcc attgagtaga gcggattaaa ggtggaaggc gctttcggca
    20281 gcccggctat cacagccatt tcgttcagcg tcagttggtc gaccgtttta ccgaaataga
    20341 cttgtgccgc agcaccgaca ccataggcgc ggtaaccgag gtaaatcttg ttcagataaa
    20401 gctcgaggat ctcgtctttc gtcagcaact gttcaatgcg aatcgcgagg aagacttcct
    20461 taatcttacg catcagcgtg cgttctggac tgaggaagaa gtttctcgcc agctgctggg
    20521 taatggtact cgccccttgt gacgcgtggc cggagaacag cgccacactt gccgcacgga
    20581 agatccccac cgggtcaacg ccgtgatgct cgtagaagcg gctgtcttcc gtcgcgataa
    20641 aggctttcac catctccggc gggatttgat ccaacgtaac cggaatacga cgtttctcac
    20701 cgtattgagc aatcagctcg ccatcggcgc tgtaaatctg catcggaatt tgcaggcgaa
    20761 catcttttaa tgtcgccaca tccggcagtt gtggctcgat gtagcggtat aggccataaa
    20821 tcgagcctgc tcccagcaga atgcaacaga ctgcaaggat caaaaaatac tttacgaact
    20881 tcactggaaa tttcccattt agtttcattt gggcagttta taaacaaacg tgcggtagta
    20941 taaaggcaag ccagacgcat tgatataccc gtcagagtga cgggtgataa ggagatcatc
    21001 acaatggcat ttaagatctg gcaaattggt ttgcatttac aacagcaaga agcagtagcg
    21061 gttgcgatcg tgcggggcgc aaaagaatgc tttttgcaac gctggtggcg gttgccgctg
    21121 gagaacgaca ttatcaaaga tgggcggatt gttgatgcgc agcggctggc taaaacgttg
    21181 ttaccgtgga gtcgcgaact gccgcagcgt catcacatta tgttggcgtt tcccgccagt
    21241 cgcacattac agcggtcatt tccgcgcccg tcgatgtccc tcggtgagcg ggagcaaacg
    21301 gcctggctgt cagggacgat ggcccgcgag ctggatatgg atccggactc cctgcgtttc
    21361 gattacagcg aagactcact cagtcccgcc tataacgtga ctgccgcgca aagcaaagag
    21421 ctggcaacgc tactcactct ggcagaaagg ttgcgtgttc atgtgagcgc gatcaccccg
    21481 gatgccagtg cattacagcg attcctgcct tttttacctt ctcatcagca atgtctggcc
    21541 tggcgtgata acgaacagtg gctgtgggcg acacgctgtc gctgggggcg caaactggcg
    21601 gtggggatga ctagcgcgaa ggagctggcg gcagcgttat ccgttgatcc cgaaagcgtc
    21661 gcgatatgtg gcgaaggcgg atttgatccc tgggaggccg tttctgttcg tcagccgccg
    21721 ctaccgccgc caggtggaga ctttgccatc gcgctggggc tggcgcttgg gaaggcgtac
    21781 tgatgaaccc gccaattaat tttttgccct ggcgacagca acgccggacc gcttttcttc
    21841 gtttctggtt gctgatgttc gttgcgcctc tgctgctggc cgtcgggata acgctaatac
    21901 tgcgtctgac agacaacgcc gaagcacgcg taaacaccgt tttgcttcag gcggaacaac
    21961 aactcgcccg cagcttacag ataacgaaac cacgtttgct ggagcgccaa caaattcgtg
    22021 agcagcgttt gcaacggcag cgccagcgac aatttacccg cgactggcaa tctgcgctgg
    22081 aagcactggc ggcgctttta cccgagcacg cctggctgac aacgataagc tggcagcagg
    22141 gaacgctgga gatcaagggg ctgacaacaa gcattaccgc gttaaacgca ctagaaacgt
    22201 cactccgcca ggatgcgtct tttcatctca atcagcgggg agccacgcag caggatgcgc
    22261 agggacgctg gcaatttgag tatcagttaa caaggaaggt tagcgatgaa catgttcttt
    22321 gactggtggt tcgccacatc accccgcctc cgccagtttt gctgggcatt ctggttgctg
    22381 atgttagtta cgctcatttt tctgtcatcg acacaccatg aagagcgcga cgcattaatt
    22441 cgactacggg caagtcatca ccagcagtgg gccacactgt atcgcctggt tgacaccact
    22501 cccttcagcg aagaaaaaac gctgcccttt tcgccactgg attttcagtt gcccggcgcg
    22561 caactggttt cctggcatcc atccgcgcag ggaggcgagt tggcgttgaa aacgctgtgg
    22621 gaagcagtgc cgtcggcatt tacacggctg gcagagcgca acgtcagcgt gagccgtttt
    22681 tcattaagcg tggaaggtga tgatcttttg ttcacgctac aactggagac gccgcatgag
    22741 ggttaaacgc tggttgttgg caggtattgt attgtgcctt ttaaccggta tgcgtgaccc
    22801 ttttaaaccg ccggaagatc tatgccggat tagcgaactt agccagtggc gctatcaggg
    22861 gatggtaggg cgaggcgagc gcatcatcgg tgtaataaaa gacgggcaaa agaaatggcg
    22921 acgggtgcag caaaacgatg tgctggaaaa cggctggaca attttacagc tgacgccaga
    22981 cgtactaacg ctgggtaccg ggacaaactg cgaaccgcca caatggttgt ggcaacggca
    23041 aggagataca aatgaagcaa tggatagccg cactactgtt gatgctgata cccggcgtac
    23101 aggcggcaaa gccgcaaaaa gtgacgctga tggtggatga cgttccggta gctcaggtgt
    23161 tgcaggcgct ggctgaacag gagaagttga acctggtcgt gtcgccagac gtcagcggta
    23221 cggtgtcgtt acatctaaca gatgttccct ggaagcaggc actacaaact gtagtgaaaa
    23281 gcgccggact gataacgcgg caggaaggca acattctctc agtgcattcc attgcctggc
    23341 agaataacaa tatcgcccgc caggaggcgg agcaggcgcg ggcgcaggca aatctgccgc
    23401 tggaaaatcg cagtataacc ctgcaatacg ccgacgcggg agaactggcg aaagcggggg
    23461 agaagctact gagtgccaaa gggagtatga ccgtcgataa acgcaccaat cgccttttgc
    23521 tacgagataa caaaacggcg ttaagcgcgc ttgaacagtg ggtagcgcaa atggatctgc
    23581 cggtcgggca ggttgagctg tcggcgcata ttgtcaccat taatgaaaaa agtttgcgtg
    23641 agttaggcgt gaaatggacg ctggccgatg cgcaacacgc tggtggcgtt gggcaagtca
    23701 ccacgcttgg tagcgacctc tccgtagcga cggcgacaac gcatgtcggt tttaacattg
    23761 ggcgcatcaa cggacgcttg ctggatcttg agctttccgc gctcgaacaa aaacagcagc
    23821 tggatattat cgccagtccg cgtctgctgg cctcacatct tcagcctgcc agcattaaac
    23881 aggggagcga aattccatat caggtttcca gcggggaaag tggcgcgacg tcggtggaat
    23941 ttaaagaggc cgtcctgggg atggaagtta cgcccacagt gttacaaaaa ggtcgcatcc
    24001 ggctgaaatt acacatcagc cagaacgttc cggggcaggt gctacagcag gccgatggcg
    24061 aagtgctggc gattgataag caggagatcg aaacgcaggt cgaggtcaaa agcggagaaa
    24121 cgttggcgct gggcggcatt tttacccgta aaaataaatc gggtcaggat agcgtaccgt
    24181 tgcttggcga cattccctgg ttcgggcaat tatttcgtca tgacggaaaa gaagatgaac
    24241 gacgcgagtt agtggtgttt atcacgccac gactggtttc cagtgagtaa acagccgtaa
    24301 aagcggtaat gtttttacgc tgaacgtgtt tcatctattt gacgcgcgca ggtatttagc
    24361 atacaaggag taccgatttg agagttggtg ctcttcgctg cctgcgttcc atgatgatga
    24421 tttatcattc aggcggcatt ttgctgtctt ttttacgcta atcttacccg gtgatttatc
    24481 gccagagcgg tggtagcaag gcagcgcgct tgcagcgacc agatatgcag agggatgggt
    24541 gatttattca gttgccaaac ccgctggagt attgagataa ttttcagtct gactctcgca
    24601 atatcttatg aggtttcagt tcatgtcctg cggcgctctc tgagcgaagc gggtttatca
    24661 ttaacgaata gtcttagtag taccgaaaaa atggcagaga aacgcaatat ctttctggtt
    24721 gggcctatgg gtgccggaaa aagcactatt gggcgccagt tagctcaaca actcaatatg
    24781 gaattttacg attccgatca agagattgag aaacgaaccg gagctgatgt gggctgggtt
    24841 ttcgatttag aaggcgaaga aggcttccgc gatcgcgaag aaaaggtcat caatgagttg
    24901 accgagaaac agggtattgt gctggctact ggcggtggct ctgtgaaatc ccgtgaaacg
    24961 cgtaaccgtc tttccgctcg tggcgttgtc gtttatcttg aaacgaccat cgaaaagcaa
    25021 cttgcacgca cgcagcgtga taaaaaacgc ccgttgctgc acgttgaaac accgccacgt
    25081 gaagttctgg aagcgttggc caatgaacgc aatccgctgt atgaagagat tgccgacgtg
    25141 accattcgta ctgatgatca aagcgctaaa gtggttgcaa accagattat tcacatgctg
    25201 gaaagcaact aattctggct ttatatacac tcgtctgcgg gtacagtaat taaggtggat
    25261 gtcgcgttat ggagaggatt gttgttactc tcggggaacg tagttaccca attaccatcg
    25321 catctggttt gtttaatgaa ccagcttcat tcttaccgct gaaatcgggc gagcaggtca
    25381 tgttggtcac caacgaaacc ctggctcctc tgtatctcga taaggtccgc ggcgtacttg
    25441 aacaggcggg tgttaatgtc gatagcgtta tcctccctga cggcgagcag tataaaagcc
    25501 tggctgtact cgataccgtc tttacggcgt tgttacaaaa gccgcatggt cgcgatacta
    25561 cgctggtggc gcttggcggc ggcgtagtgg gcgatctgac cggcttcgcg gcggcgagtt
    25621 atcagcgcgg tgttcgtttc attcaagtcc cgacgacgtt actgtcgcag gtcgattcct
    25681 ccgttggcgg caaaactgcg gtcaaccatc ccctcggtaa aaacatgatt ggcgcgttct
    25741 accagcctgc ttcagtggtg gtggatctcg actgtctgaa aacgcttccc ccgcgtgagt
    25801 tagcgtcggg gctggcagaa gtcatcaaat acggcattat tcttgacggt gcgtttttta
    25861 actggctgga agagaatctg gatgcgttgt tgcgtctgga cggtccggca atggcgtact
    25921 gtattcgccg ttgttgtgaa ctgaaggcag aagttgtcgc cgccgacgag cgcgaaaccg
    25981 ggttacgtgc tttactgaat ctgggacaca cctttggtca tgccattgaa gctgaaatgg
    26041 ggtatggcaa ttggttacat ggtgaagcgg tcgctgcggg tatggtgatg gcggcgcgga
    26101 cgtcggaacg tctcgggcag tttagttctg ccgaaacgca gcgtattata accctgctca
    26161 agcgggctgg gttaccggtc aatgggccgc gcgaaatgtc cgcgcaggcg tatttaccgc
    26221 atatgctgcg tgacaagaaa gtccttgcgg gagagatacg cttaattctt ccgttggcaa
    26281 ttggtaagag tgaagttcgc agcggcgttt cgcacgagct tgttcttaac gccattgccg
    26341 attgtcaatc agcgtaacaa caagaaaggt caggccgctt atcaagcggt ctattagctt
    26401 caggttaatt gcaacgtggt aagcattaac cttttagtgg ggtgttaaat ggatgaattc
    26461 aaaccagaag acgagctgaa acccgatccc agcgatcgtc gtactggtcg ttctcgtcaa
    26521 tcttctgaac gttctgagcg tactgaacgt ggcgaaccgc agatcaattt tgatgatatt
    26581 gaacttgatg acactgacga tcgccgtccg actcgtgcgc aaaaagagcg caatgaggaa
    26641 ccggaaatcg aagaagaaat tgacgaatcc gaagatgaaa ccgtggatga agagcgcgta
    26701 gagcgtcgtc cgcgtaagcg caaaaaagca gccagtaaac ccgcttctcg tcagtatatg
    26761 atgatgggcg tcggcattct ggttctactg ctgttgatca tcggtatcgg ttctgcgcta
    26821 aaagccccct cgacctcttc cagcgatcaa accgcgtctg gcgagaagag tattgatctt
    26881 gcaggcaatg cgaccgatca ggcgaatggg gtgcagccag cgccgggaac cacgtctgcg
    26941 gaaaatactc agcaggatgt ttctctgcca ccgatctctt ctacgccgac tcaagggcaa
    27001 accccggcgg caacggatgg tcaacaacgt gttgaagtgc agggtgacct gaacaatgcg
    27061 ctgacccagc cacaaaatca gcaacagttg aacaatgtgg cggtcaattc cacattgccg
    27121 accgaacccg cgactgtcgc gcctgttcgc aatggcaatg catcgcgtga cacggcgaaa
    27181 acgcaaaccg ctgaacgtcc gtccactacg cgcccagctc gtcagcaggc ggtgattgaa
    27241 ccgaaaaaac cgcaagcaac cgtgaaaacg gagccgaagc cggtagcaca gacgccgaag
    27301 cgtactgaac cagctgcccc tgtggcgagc acgaaggcac cgactgcgac ttctacgcca
    27361 gcaccaaaag agacggcgac tacggctcca gtacagacgg catccccggc gcaaaccacg
    27421 gcaacaccag ccgctggagg gaagaccgca ggtaatgttg gttcgttgaa atcggcaccg
    27481 tccagccatt acactctgca gctgagcagt tcctctaact acgacaacct gaacggttgg
    27541 gcgaagaaag agaatctgaa aaactacgtt gtctatgaaa cggcgcgtaa tggtcagccg
    27601 tggtatgtcc tggtttctgg cgtgtacgct tcgaaagaag aggcgaaaaa agcggtatct
    27661 acattgccag cagatgttca ggccaaaaac ccgtgggcga aaccgctgcg tcaggtacag
    27721 gccgatctga agtaatcaag gttatctccc gcaatggttt atcgttgcgg gagctgcctg
    27781 aagcgctgga tgctgtcgga gctttctcca cagccggaga aggtgtaatt agttagtcag
    27841 catgaagaaa aatcgcgctt ttttgaagtg ggcagggggc aagtatcccc tgcttgatga
    27901 tattaaacgg catttgccca agggcgaatg tctggttgag ccttttgtag gtgccgggtc
    27961 ggtgtttctc aacaccgact tttctcgtta tatccttgcc gatatcaata gcgacctgat
    28021 cagtctctat aacattgtga agatgcgtac tgatgagtac gtacaggccg cacgcgagct
    28081 gtttgttccc gaaacaaatt gcgccgaggt ttactatcag ttccgcgaag agttcaacaa
    28141 aagccaggat ccgttccgtc gggcggtact gtttttatat ttgaaccgct acggttacaa
    28201 cggcctgtgt cgttacaatc tgcgcggtga gtttaacgtg ccgtttggcc gctacaaaaa
    28261 aacctatttc ccggaagcag agttgtatca cttcgctgaa aaagcgcaga atgccttttt
    28321 ctattgtgag tcttacgccg atagcatggc gcgcgcagat gatgcatccg tcgtctattg
    28381 cgatccgcct tatgcaccgc tgtctgcgac cgccaacttt acggcgtatc acacaaacag
    28441 ttttacgctt gaacaacaag cgcatctggc ggagatcgcc gaaggtctgg ttgagcgcca
    28501 tattccagtg ctgatctcca atcacgatac gatgttaacg cgtgagtggt atcagcgcgc
    28561 aaaattgcat gtcgtcaaag ttcgacgcag tataagcagc aacggcggca cacgtaaaaa
    28621 ggtggacgaa ctgctggctt tgtacaaacc aggagtcgtt tcacccgcga aaaaataatt
    28681 ctcaaggaga agcggatgaa acagtatttg attgccccct caattctgtc ggctgatttt
    28741 gcccgcctgg gtgaagatac cgcaaaagcc ctggcagctg gcgctgatgt cgtgcatttt
    28801 gacgtcatgg ataaccacta tgttcccaat ctgacgattg ggccaatggt gctgaaatcc
    28861 ttgcgtaact atggcattac cgcccctatc gacgtacatc tgatggtgaa acccgtcgat
    28921 cgcattgtgc ctgatttcgc tgccgctggt gccagcatca ttacctttca tccagaagcc
    28981 tccgagcatg ttgaccgcac gctgcaactg attaaagaaa atggctgtaa agcgggtctg
    29041 gtatttaacc cggcgacacc tctgagctat ctggattacg tgatggataa gctggatgtg
    29101 atcctgctga tgtccgtcaa tcctggtttc ggcggtcagt ctttcattcc tcaaacactg
    29161 gataaactgc gcgaagtacg tcgccgtatc gacgagtctg gctttgacat tcgtctggaa
    29221 gtggacggtg gcgtgaaggt gaacaacatt ggcgaaatcg ctgcggcggg cgcggatatg
    29281 ttcgtcgccg gttcggcaat cttcgaccag ccagactaca aaaaagtcat tgatgaaatg
    29341 cgcagtgaac tggcaaaggt aagtcatgaa taagtttgaa gatattcgcg gcgtcgcttt
    29401 tgatcttgat ggtacgctgg tcgacagtgc tcccggtctt gctgctgcgg tagatatggc
    29461 gctgtatgcg ctggagttgc ccatcgcagg tgaagaacgc gttattacct ggattggtaa
    29521 cggcgcagat gttctgatgg agcgcgcatt gacctgggcg cgtcaggaac gtgcgactca
    29581 gcgtaaaaca atgggtaaac cgcccgttga tgacgacatt ccggcagaag aacaggtacg
    29641 tattctgcgt aaactgttcg atcgctacta tggcgaggtt gccgaagagg ggacgttttt
    29701 gttcccgcac gttgccgata cgttgggcgc gttgcaggct aaaggcctgc cgctaggcct
    29761 ggtcaccaac aaaccgacgc cgttcgtcgc accgctgctc gaagccttag atattgccaa
    29821 atactttagc gtggttatcg gcggcgatga tgtgcaaaac aaaaaaccgc atccggaccc
    29881 gctgttactg gtggctgagc ggatgggaat tgccccacaa cagatgctgt ttgtcggcga
    29941 ctcacgcaat gatattcagg cggcaaaagc ggcaggttgc ccatcagttg gcttaaccta
    30001 cggatataac tacggcgagg ctatcgatct cagccagcct gatgtaattt atcagtctat
    30061 aaatgacctt ctgcccgcat tagggcttcc gcatagcgaa aatcaggaat cgaaaaatga
    30121 ctaagcccat cgtttttagt ggcgcacagc cctcaggtga attgaccatt ggtaactaca
    30181 tgggtgcgct gcgtcagtgg gtaaacatgc aggatgacta ccattgcatt tactgtatcg
    30241 ttgaccaaca cgcgatcacc gtgcgccagg atgcacagaa gctgcgtaaa gcgacgctgg
    30301 atacgctggc cttgtatctg gcttgtggta tcgatcctga gaaaagcacc atttttgttc
    30361 agtcccacgt accagaacat gcgcagttag gctgggcact gaactgttat acctacttcg
    30421 gcgaactgag tcgcatgacg cagtttaaag ataaatctgc gcgttatgcc gagaacatca
    30481 acgctggtct gtttgactat ccggtgctga tggctgcgga catcctgctg tatcaaacta
    30541 atctggtacc ggtgggtgaa gaccagaaac agcacctgga actgagtcgc gatatcgccc
    30601 agcgtttcaa cgcgctgtat ggcgagatct ttaaggtgcc ggagccgttt attccgaaat
    30661 ctggcgcgcg cgtaatgtcg ctgctggagc cgaccaagaa gatgtccaag tctgacgata
    30721 accgcaataa cgttatcggc ctgctggaag atccgaaatc ggtagtgaag aaaatcaaac
    30781 gcgcggtcac tgactccgac gagccgccgg tagttcgcta cgatgtgcag aacaaagcgg
    30841 gcgtttccaa cctgctggat atcctttctg cggtaacggg ccagagcatc ccagaactgg
    30901 aaaaacagtt cgaaggcaag atgtatggtc atctgaaagg tgaagtggct gatgccgttt
    30961 ccggtatgct gactgaattg caggaacgct atcaccgttt ccgcaacgat gaagccttcc
    31021 tgcaacaggt gatgaaagac ggcgcagaaa aagccagtgt acacgcttcc cgtacgctga
    31081 aagcggtgta cgaagcgatt ggttttgtgg cgaagccgta atttaacatt aatgccaaaa
    31141 accggggaaa cccggttttt ttatcctcat ttgcaataat ccgaaaaaat gtgaagcgcc
    31201 tcgccgtttc cacatctcgt cgttgccact tatcttcatt ttaatgaaga tataaatatt
    31261 cattttattg aaaatatatt atgcgtcgaa cgtttatcaa aaaagaaggc gtcgtcatca
    31321 tgacgctggc ccgttatttg ttgggtgaaa aatgcggtaa tcgactgaaa accatagatg
    31381 agctggcaac tgaatgccgt tcatcggttg gcctgacgca ggccgcgttg aaaacgttgg
    31441 aatcaagcgg agcgatacgg attgaacgcc gtgggcgcaa tggcagttat ctggtcgaga
    31501 tggataacaa agcattgctg agtcatgtgg atatcaacaa cgtggtatgt gcgatgcctt
    31561 tgccctatac ccgcttgtac gaaggtttag cgagcgggct gaaagctcag tttgatggca
    31621 ttccttttta ctatgcgcat atgcgcggcg cggatattcg cgtggagtgt ttacttaacg
    31681 gcgtgtatga catggcggtg gtttcccgac tggcggcgga aagttatctc acgcaaaaag
    31741 ggttatgcct cgcgctggag ttggggccgc acacctacgt tggcgagcac cagttgattt
    31801 gccgtaaagg cgagtccgca aacgtgaagc gcgtggggct ggataaccgt tcggcggatc
    31861 agaaaatcat gaccgatgtc ttttttggcg atagtgatgt ggaacgtgtc gatctctctt
    31921 atcacgagag tttacaacgc attgttaaag gcgatgtcga tgcggttatc tggaacgtgg
    31981 tggcggaaaa cgaactgacc atgctgggat tagaggcgac gccgctcaca gacgatccgc
    32041 gatttttaca ggccaccgaa gccgtggtcc tgacgcgagt cgatgattac ccaatgcaac
    32101 aactgctgcg tgccgttgta gataaacacg ccctgctcgc ccatcaacaa cgggtagtga
    32161 gtggggaaca ggaaccgagt tattaaacga aaggcatctg atatggaaac cagactcaac
    32221 ctgctttgcg aggcaggcgt tattgataag gacatctgca aaggcatgat gcaggtcgtc
    32281 aacgtactgg aaacagagtg ccatctgccg gtgcgcagtg agcaaggaac gatggcgatg
    32341 acacatatgg cgagtgcact gatgcgcagt cgccgtggtg aagaaataga gccgctggat
    32401 aacgaattgc tggcagagct ggcgcaatcc agccactggc aagccgttgt gcaattgcat
    32461 caggtgttgt tgaaggaatt cgcactggaa gttaacccgt gtgaagaagg ctatttgctg
    32521 gccaaccttt atggattatg gatggctgct aacgaagagg tttgaagctc ccgacaaatc
    32581 cacgtcgcac aacctaaata ttcaaaaaaa tgaatattta attcaaggaa cagaaacgag
    32641 gcaacaagat gtttgtagaa gcattgaaac gccagaaccc ggcgctgatt tccgccgcac
    32701 taagtttgtg gcagcagggc aagatcgccc cggacagctg ggtgatcgac gtggatcagg
    32761 tactggaaaa cggtaagcga ctgattgaga cggcgcggct ttacggcatt gaactgtatc
    32821 tgatgaccaa gcagtttggc cgtaacccat ggctggcgga aaaattgctg gcattaggtt
    32881 acagcggcat tgtggcggtg gattacaaag aggcgcgagt catgcgccgc gctggtttgt
    32941 cggtggcaca tcaggggcat ctggtacaaa tcccttgtca tcaggttgct gacgccgttg
    33001 aacagggcac cgacgtcatc accgtgttta ctctcgacaa agcgcgggaa gtttctgcgg
    33061 cagcggtgaa ggccggacgt gtgcagtttg tgctgctcaa agtttatagc gacgatgatt
    33121 ttctttatcc ggggcaggag agcggttttg tacagcattc gctgcacgag gtggttgctg
    33181 aaattaagaa actgccgggg ctgcatttag ccggacttac ccatttccct tgcctgcttt
    33241 gggatgaggc tgccggaaaa gttttgccga caccgaatct tcacacgctg atacaggcac
    33301 gggatcaact ggcgaaatct ggtattgcac ttgagcaact gaacgcgcct tcagcgacca
    33361 gctgcacttc gctgccatta ctggcgtaat acggtgtgac tcatgccgaa cccggtcatg
    33421 cgctgacggg cactattccg gcaaaccagc agggcgatca gtctgaacgt atcgcgatgc
    33481 tctggttgag tgaaatctcc catcatttcc gtggcgacag ctactgctac ggcggcggtt
    33541 actaccgtcg tggtcatgcg caacatgcgt tggtgtttac gccagaaaat caaaagatta
    33601 ctgaaaccaa tctcaaaact gtggatgaca gcagtatcga ctacaccctg tcgctggcag
    33661 gcgagtttcc ggtaagcagt gcagtggtgc tctgtttccg cacgcagatt tttgtcaccc
    33721 gtagcgatgt ggtgctggtg tccggtattc accgtggcga accgaaaata gttggtcgtt
    33781 atgacagtct tggaaactct ctgggggcgt aatggcgcga tttgtggtgt tagtgattga
    33841 tagctttggc gtaggggcaa tgaaagatgt cacgctggtg cgtccgcaag atgcgggagc
    33901 gaatacatgt ggtcacatcc tgagccagtt gccgcatttg cagctaccaa cgctggagaa
    33961 gctggggcta atcaacgcat tgggttatgc gccaggcgat atgcagccgt cagattccgc
    34021 aacctggggc gtggcagagc tgcaacatga aggtggcgat acctttatgg ggcatcagga
    34081 aattttaggc acgcgcccgt taccgccgct gcggatgcct tttcgcgatg tgattgaccg
    34141 tgttgagcag gcattagtat ccgccggttg gcaggtggag cgccgtggcg atgaactgta
    34201 atttctgtgg gtcaatcagg cggttgcgat tggcgataat ctcgaggcgg atttaggcca
    34261 agtctataac attaccgcca atctctctgt gatctctttt gacgacgcaa tcaaaattgg
    34321 tcgtatcgtg cgtgagcagg tacaggtcgg tcgggtcatt acatttggtg gcctgttaac
    34381 cgacagtcaa cgcattctcg atgccgcaga aagcaaagaa gggcgcttta ttggtatcaa
    34441 tgcgccgcgt tctggcgctt atgacaacgg cttccaggtc gtgcatatgg gctacggtgt
    34501 cgataaaaaa gtgcaggtgc cgcaaaaact gtatgaggcg ggcgtgccaa ccgtgctggt
    34561 gggtaaggtg gcagatatcg tcagcaatcc ttatggcgtg agctggcaaa acctggtgga
    34621 tagccagcgg attatggata tcaccctcga cgaatttaac acccatccga cggcgtttat
    34681 ttgcaccaat atccaggaaa ccgacctcgc tggtcatgca gaagacgtcg cacgttatgc
    34741 cgaacgtttg caggtcgttg accgtaacct tgcccggctt gttgatgcga tgcagccaga
    34801 tgattgcctg gtcgtgatgg cggatcacgg caacgatccg accattggtc acagccacca
    34861 tacccgcgaa gtggtgccgc tgctggttta tcagcaaggc ctggctgcta cgcagctcgg
    34921 tgtgcgcacc acgctttctg atgtgggagc taccgtgtgt gaatttttcc gtgcgccacc
    34981 gccacaaaat ggtcgctctt ttctttcctc cctccggttt gcaggagaca ccctatgagt
    35041 tttgatccga cgggttacac cctggcccat gagcatctgc atattgatct ctccggcttt
    35101 aaaaacaacg tggactgccg ccttgatcag tatgcgttca tttgccagga gatgaacgac
    35161 ctgatgaccc ggggtgtacg taatgtgatt gagatgacca accgttacat ggggcgcaat
    35221 gcgcaattta tgctcggtgt aatgcgcgaa accgggatca acgtggtggc ctgtactggt
    35281 tattaccagg atgcgttttt cccggaacat gtggtgaccc gcagcgtgca ggaactggcg
    35341 caggagatgg tcgatgaaat tgaacagggt atcgatggca cggagctgaa agccgggatc
    35401 atcgcggaga tcggctccag cgaaggaaag attacgccgc tggaagagaa ggtatttatt
    35461 gccgctgcgc tggcgcataa ccagaccgga cgcccgatct ccacgcatac gtcgttcagc
    35521 accatggggc tggaacaact ggctctgttg caagcccacg gagttgatct ttcgcgcgtc
    35581 accgttggtc actgcgatct gaaagacaac ctcgacaaca ttttgaagat gatcgatctc
    35641 ggcgcgtacg tgcagttcga caccattggt aagaacagtt actacccgga cgaaaagcgc
    35701 attgcgatgc tccacgcgct acgtgaccgt gggttgctga accgcgtcat gctgtcgatg
    35761 gatattacgc gccgctccca tttaaaagcc aatggtggtt atggctatga ctatttattg
    35821 accaccttta ttccgcaatt gcgccagtca ggattcagtc aggccgatgt ggatgtgatg
    35881 ttacgtgaaa ctccctctca atttttccaa taaggacaga ctcatgaaaa agattggcgt
    35941 tgcaggctta cagcgtgagc agattaaaaa aaccattgaa gcgacggctc ctggcagttt
    36001 tgaagttttc attcacaacg acatggaagc ggcaatgaaa gtgaaatccg ggcaactgga
    36061 ttactacatc ggtgcgtgta ataccggtgc gggcgcggca ttgtcgattg ccatcgcggt
    36121 gattggctat aacaaaagtt gcaccattgc caaaccaggc attaaagcga aagacgagca
    36181 tatcgccaaa atgatcgctg aaggaaaagt ggcgtttggc ctttccgttg agcacgtcga
    36241 acacgcgatt ccgatgctga ttaaccatct gaaataaaag gcacgactat ggatctgtat
    36301 attcagatta tcgtggtggc gtgcctgacg ggtatgacat cgcttctggc gcatcgctcg
    36361 gcggctgttt ttcatgacgg tatccgcccg atcctgccgc aactgattga aggctatatg
    36421 aaccgtcgcg aggcggggag tatcgctttt ggtctgagca ttggttttgt ggcctcggtg
    36481 gggatctctt ttaccctgaa aaccgggctg ctcaacgcat ggttactctt tcttcctacc
    36541 gatatcctcg gcgtactggc gataaacagc ctgatggcgt ttggtcttgg cgctatctgg
    36601 ggcgtgttga tccttacttg cctgttgcca gtaaaccagc tgctgaccgc gctgccggtg
    36661 gatgtattag gtagcctcgg ggaattaagc tcgccggtgg tttctgcttt tgcactgttc
    36721 ccgctggtgg cgattttcta ccagtttggc tggaagcaaa gtctggtcgc cgccgtggtt
    36781 gttctgatga cccgtgtggt agtcgtgcgc tatttcccac atcttaaccc tgaatccatc
    36841 gaaatcttta ttggcatggt gatgctgctg gggatcgcga taactcacga cctgcgtcat
    36901 cgtgatgaaa atgatattga tgccagcggg ctttcggtgt ttgaagaacg cacgtcgcgg
    36961 attatcaaaa acttacccta tatcgccatc gtgggagcat tgattgccgc cgttgccagc
    37021 atgaagattt tcgccggcag tgaagtgtcg atcttcactc tggagaaagc gtactccgca
    37081 ggcgtaacgc cggaacaatc gcaaacgctg atcaaccagg cggcactggc agaatttatg
    37141 cgcgggctgg gttttgtacc gttgattgcc accaccgcct tagcaacggg tgtgtatgca
    37201 gttgcgggct ttacctttgt ttatgcggtg ggctatctct cgccgaatcc gatagttgca
    37261 gcggtattag gcgcagtggt tatttcggcg gaagtcttgc tgcttcgttc gatcggcaaa
    37321 tggctgggac gctacccgtc ggtgcgtaat gcgtcggata acatccgtaa cgccatgaat
    37381 atgctgatgg aagtggcgct gctggtcggt tcgattttcg cagcaattaa gatggcgggt
    37441 tataccggat tctctatcgc ggttgccatt tacttcctca acgaatccct gggccgtccg
    37501 gtacagaaaa tggcggcacc ggtcgtggca gtaatgatca ccggtattct gctgaatgtt
    37561 ctttactggc ttggcctgtt cgttccggct taaggaaggc acctatgaag acgtttcctc
    37621 tgcaaagtct gacgattatt gaggcgcagc aaaagcagtt tgcgctggtg gatagcattt
    37681 gtcgccattt ccccggcagc gagtttctta ctggcggtga tttaggctta acgccaggac
    37741 tgaatcaacc gcgcgttacc cagcgtgtgg aacaggtgct ggctgatgca tttcacgcac
    37801 aggctgcggc gctggtgcag ggtgcgggta ctggcgcgat tcgtgcggcg ttggcggctt
    37861 tgctcaaacc ggggcagcgt cttctggtac atgatgcgcc tgtttacccg acgacacagg
    37921 tcattattga gcagatgggg ctgacgctta ttactgctga tttcaatgac ctgtcggcac
    37981 tgaagcaggt cgtcgacgag caactaccgg atgcggcgct ggtgcagcat acgcgccagc
    38041 agccgcagga cggctacatt ctggcagatg tgctggcaac attgcgctcg gctggcgttc
    38101 ctgcattaac cgatgacaac tatgcggtga tgaaagtggc gcgaattggc tgcgaatgcg
    38161 gggcaaatgt ctcgactttt tcctgcttca agctgtttgg gccggagggt gttggcgcgg
    38221 tggtaggcga tgccgatgtt attagtcgga ttcgctccac gctctattcc ggcggcagcc
    38281 agattcaggg cgctcaggcg ctggaagtct tgtgtgggct ggtgtttgcg ccagtgatgc
    38341 acgcggtgca ggcgggggta tctgaacggt tgctggcttt gcttaacggt ggtgcggtgc
    38401 cggaagtgaa aagcgcggtg attgctaatg cgcagtcgaa ggtgttgatt gtcgagtttc
    38461 atcagccgat tgccgccaga gtgctggaag aggcgcaaaa gcgcggtgcc ttgccttacc
    38521 cggtgggtgc agagtcgaaa tatgaaatcc cgccgctctt ttatcgcctt tccggaacgt
    38581 ttcgccaggc gaatccacaa tcagaacatt gtgcgattcg cattaacccg aatcgcaacg
    38641 gtgaagagac ggtgctgcgg attttgcgtg agagtattgc cagtatttaa tgctggatag
    38701 caccgtcaag ctaaattccg tactgaacgg tcccctcgcc cctttgggga gagggttagg
    38761 gtgaggggaa cgtacgggat gtaaaattca acgcgccagc atcgccggat gcgatgctgg
    38821 cgcatcttat ccggcctacg gttaatgtct tttattatca atcgttaagg tgatgcgatt
    38881 ggcgcggcag aagagttcgg agacgtgcac cggtttgtca ttctgatcaa aggcgatttt
    38941 gctgatgcga aacaacggtt cgcccagttc gcatttcagc cattttgcct gcgggcgggt
    39001 ggcggcaaag atatcgatgg tctttttgtc gctgaccact cgcgtgtcaa aacgctcctg
    39061 aaataactga taggtggagc ttccttcgac gtaaatctca tcaaagtcag gataacgcga
    39121 cagcgggatc catgaactat caataaacag cggctcttta tcgagataca tcacccggca
    39181 gagatggaac acttcgctgt ttccggggat gttcagcttt tcgcaaaacg gcgcggcgct
    39241 gacccgttcc tgttcgatca ctttctcttt cgtcgccttg ccttgtgaga cgccaaaatc
    39301 ggtaaaacca ctgacagtaa gcagggcgtt ttcaactttc tggctttgta caaaggtgcc
    39361 tttgccctgc cagcggatca gtacgccgtc tgccactaag tcgctgatag ctttgcgaat
    39421 agtaatgcgg ctgacgttat attgcgtaca aagctcgttt tcggtaggga tctgttgccc
    39481 ggcctggtaa accccctgcg cgatatcatc cagcagccgc tggcggacgg tagcgtagag
    39541 gagttgatga gagtagcggt ccgtagctga cataatcgac cctgaaataa agaatgaatt
    39601 acacggatta tagggtcgtt ttccggcaag taacagcacg ttggcgcgga gatgctcacg
    39661 ccaacgttat acctaccagg caccgtgata ctgaatggtt ttcgccgcgc acgccgttcc
    39721 ctgcgccatc gcctgcggta atgtcatccc cgcagaccag ccgcaaagga atccggcaat
    39781 gaacgaatct ccggcaccca tggtgtcgat aaccgtcacc ggttcaggag cctgacgcca
    39841 gaactgcgcg ccatcccagg caatgctgcc gttttcaccc agcgtgacaa tcactgttcc
    39901 tgcgccacgg gcaacaatcg ctttcatctt cagacgcagc gcttcgtctt cttgcggtgc
    39961 ggaggcaaag gcaaaatcga gatgcggcac cagtgtctgc cagagcgggc tgtcccactt
    40021 gtcggagaag tcgaaagcgg taagtttgcc cgcagcgtgc agctgtggga atgcgtcttc
    40081 cgcatgtccc cagattgccg cgtgcacaat gtcatactgc gccagccagg cgtaatcctc
    40141 ttcactcagg gcaaagtcgg ccatcacgcc ttcggtgtag tcgccaaaaa cgcgatcatt
    40201 gtcgtgcagt tccacctgag tttgtgcggt aacgccgtgt ttcgtatgga catggctgat
    40261 atcgacgccc atgcgggcga gatcctgctt cagctttgtg ccgtagtcat cgtcacccac
    40321 caaggtaatg catcccggct gtatgccgta gcgagtgcag tacaccgcca cattgaccgc
    40381 attaccgcca gaaaacgctt tattcagttg cgggtagata tcgacgcagt tatcgccgat
    40441 tgtcgccagg gttttcatct ctcatcctcc ggcagcagcg cgcgaaagcg ttccagcgcc
    40501 tggcgggcat agagtctggg ctcgttcata tacatcgtca ccagctccac cgtacagtaa
    40561 ccctcatagc cccgctcaat aatatcgcgc atcagttccc gcagcggcat tttgccttct
    40621 ccaggaatgt aatgcgtgtc gctggccccg tcgctgtcga caatatgcag atgacgataa
    40681 tttatcgccc agtttgtcga aataactcat caccggttcc gcctggacat acggcgcgca
    40741 aatgtcgacc atgctgaaca ggcgcggcga aggcaccagc gccagcgcat gaagcacatc
    40801 attagcatta cacacgacgt tcgattcata cggcgttaac ggttcgagga ttagatccat
    40861 gccgatgttt tccgcgtact cacacagttc gcttaggttc tctgccagcc gtccccagat
    40921 aacattaggt ggcgtgagat agcctgcgtg ggccgcggaa atcagcgtat aacccgcgtt
    40981 catctctttt gccatatcca tcgccagctt gatcatgtcg aggctttcgc gacgcatatg
    41041 ttcatcgccc agcatcatgt tatacggata gccgttggtt tctggcgtat agccgataat
    41101 cggcatctga tacgtctgcg ccagcgcctt gatttgtttg atgccgcccg cttttaagtc
    41161 cggcgcgaac gcgtgcgggc gaccgcccca aatttcgatg ccgtcgtaac ccagctcgct
    41221 tgcatcacga aatgcgtgtt caataggcag ccgctggtgg ccgcaggtaa acatacctgt
    41281 tttcatcggg taactccttg cgtaaatccg gcccgttgcc gggccgggag agggattaat
    41341 attccaccag accaccgtaa tagcggcgtt catccgggtt gtgatctttg taaatagaca
    41401 ggtagtagca gagccactcc attggcacga acatcaggaa cggtgccagc cacgggtgca
    41461 gcccttgcga aatttcggcg taatcgatga cgatcacgtt gtcagtacgc tgtttaacaa
    41521 agttaatggc gcgttcggtg gtgtggcgac tttcatcatt gccgagcagg aacaggaacg
    41581 gaacgcccgg ttcgacaatc tccagcgggc catggcggaa ctctccgctc tcaatcacgc
    41641 agccgtgcgt ccaggtaaat tccatcagcg ttacaatgcc ttctttgtaa cccagcggac
    41701 gcagcggacc cgcagcaacg gtataaatca tcggccactg gctggccagt tcaccaagct
    41761 ggcggccttt ttcttcccag gtgcgtacca gatgaccgag cgcattcggc aacggcttga
    41821 gatcgttttt gatcttgccg atttccgcgt tcggcgcgag gcgggtgatc atctccagca
    41881 ccacgctgta gcagagcagc aggtgaattt cccagataca gtcggcctga taatcaatgc
    41941 taaattccgc cgccgaggta atcgggctgt ccgcgcgttt ggtgaacgcc gcagtgagtg
    42001 cgccgcaggc ccggcccagc tccagcgctt tgattacctc ttccgtttta ccgtagtcag
    42061 aaacgccaat caccgcgcaa cgatcgtcga ggcgatacgg ggtgttatcg cagaactccc
    42121 agccggaaat ggcgtagacc tgaagatcgg aaaagcgatc cgccagatgt ttcgccgttt
    42181 gcgccgcgtt gagtggcgag ccgcaggcaa cgaaataaat acgatcaatg tcgcgcttca
    42241 ccatctcttc cacgatggcg tgcaccagcg gaacgtcatg gctgagaact ttttccactt
    42301 cctgaaccat attttcggtg accagaaagt ccacggtgct tttatcaata tccaacattt
    42361 tattttctcc agataacatt acgacaaagc gttgagctgg cggctacgct tcgcccagaa
    42421 ggcgtaagca ggcagaccag tagcaataac gatgacggcg cagataaggc cgggaatcgg
    42481 tgcccagaca aaggttgagg cgaccagaat gaggcttgac gcaatggcga gggtggtcat
    42541 cagcccgaaa gccggagtac gccacagcgg tttgtaatcg tcgcgtttac gacaccagat
    42601 gatggagccg aaggtgaggg tatttttgaa acacatcacc agggtgaaat aacccagcag
    42661 gctggtgaga tcggaaacga agatgaagaa gatccccagc gccccttgca ggatgatgga
    42721 gacatccggc gtgttgtatt tcggatgcac atggccgaag catttaaaga acaggttgtc
    42781 tttcgccatc gcgtattcca gccgcggctg gtacatcacg cagctggaaa gcgaaccaag
    42841 aatgacgatc atcgccgtga tggcaacaaa gatcccagcg gtgctgccga gtgcggggat
    42901 ccaggtcagg gcgtcggaaa tcggcgtttc agaattggcg agtttgtcga agggcatcag
    42961 gccggaaatc accagcgcca gcagggtgta gagcaccaga accagcagac aggaaccaat
    43021 cagcgctcgt ggcatggttt ttccggggtt tttaatttcg ccggtcatat aacagataga
    43081 ggccatgccg gtatacgacc aactggtggc agagatcccc gccagcagcg ccataaagct
    43141 gcccgttgcg ccaatcgcag tggtggtagg ggcggcaaaa ttctccgctt taaaccagaa
    43201 gatcccaagg ccaatgacga tagtgaacgg gataattttg gcgatggtaa ttagcgtctg
    43261 aaacgctgcg ccgccttcaa cggagcgcag gtgtagcaac ataaaggcga taattaatcc
    43321 ggcggcgata aatttaccga gcaacggatc gataggcgtt aaaaagccaa gattgctgac
    43381 aatcgccagc gccataatcg acaatgacgg tgcatcgttg gcccagaagc tggcccagcc
    43441 ggagaggaaa gccagcggtc ggcttccggc atttttcaga taaacataat ctgcgccatt
    43501 ttccggataa gcggtggata gttccgcata gacgcacatt tgcgggatca caattaaccc
    43561 gccaatgaca aacgcgagca ccgtaagcca cggcgtgccc gctgcttttg ccacttcacc
    43621 cacagataca aaaataccgg agccgacggt tgtcccgacg gcgattgcaa gaacggccca
    43681 aaatccgagc ttgcgttgga gttcctggct tcccatagcg ataccttttt cttgtccaat
    43741 acgttgtagg ggagagtttg cagtcattta aaaggtaaca tgacaatgca tgatgaatat
    43801 aacaacatat gatgttatgt aatgtggagg aagatcacaa aatttcgcac aggatcgcgc
    43861 tgtggctaat ggatgtagtt atcaaattga atttaaagtg aaaatatttt tacgggcggg
    43921 ggcaagaagg acatagaaac aaatacgccc tcggaaaatc cagagggcgt cgggcaatta
    43981 aaccggtgtt agccgatttc tgtcagagac ttactgtgca gtaggaccgc aaccgccaat
    44041 gattttagaa atagaaattg ccgggtgcag caggtagtcg taagagcagt tcttatcacg
    44101 gttttcaata tgaccagtac atgcagttaa cgtagccagt actgcaccta ccagtgcaac
    44161 tttaataaat ttg
//
LOCUS       DAZRUF010000008        43293 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZRUF010000008 DAZRUF010000000
VERSION     DAZRUF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.008"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..498
                     /locus_tag="ACHXOL_000354"
     CDS             <1..498
                     /locus_tag="ACHXOL_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR6807432.1"
                     /translation="LLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETKTVNII
                     SILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAVGAA
                     AGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQPNTK
                     CPEKS"
     gene            514..1053
                     /locus_tag="ACHXOL_000355"
     CDS             514..1053
                     /locus_tag="ACHXOL_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR6807433.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1148..2725)
                     /gene="guaA"
                     /locus_tag="ACHXOL_000356"
     CDS             complement(1148..2725)
                     /gene="guaA"
                     /locus_tag="ACHXOL_000356"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR6807434.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2794..4260)
                     /gene="guaB"
                     /locus_tag="ACHXOL_000357"
     CDS             complement(2794..4260)
                     /gene="guaB"
                     /locus_tag="ACHXOL_000357"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR6807435.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4422..5792
                     /gene="xseA"
                     /locus_tag="ACHXOL_000358"
     CDS             4422..5792
                     /gene="xseA"
                     /locus_tag="ACHXOL_000358"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR6807436.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5789..6004)
                     /locus_tag="ACHXOL_000359"
     CDS             complement(5789..6004)
                     /locus_tag="ACHXOL_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR6807437.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6073..7545)
                     /gene="der"
                     /locus_tag="ACHXOL_000360"
     CDS             complement(6073..7545)
                     /gene="der"
                     /locus_tag="ACHXOL_000360"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR6807438.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7663..8841)
                     /gene="bamB"
                     /locus_tag="ACHXOL_000361"
     CDS             complement(7663..8841)
                     /gene="bamB"
                     /locus_tag="ACHXOL_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR6807439.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8852..9472)
                     /gene="yfgM"
                     /locus_tag="ACHXOL_000362"
     CDS             complement(8852..9472)
                     /gene="yfgM"
                     /locus_tag="ACHXOL_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR6807440.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9490..10764)
                     /gene="hisS"
                     /locus_tag="ACHXOL_000363"
     CDS             complement(9490..10764)
                     /gene="hisS"
                     /locus_tag="ACHXOL_000363"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR6807441.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10875..11993)
                     /gene="ispG"
                     /locus_tag="ACHXOL_000364"
                     /gene_synonym="gcpE"
     CDS             complement(10875..11993)
                     /gene="ispG"
                     /locus_tag="ACHXOL_000364"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR6807442.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12020..13033)
                     /gene="rodZ"
                     /locus_tag="ACHXOL_000365"
     CDS             complement(12020..13033)
                     /gene="rodZ"
                     /locus_tag="ACHXOL_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR6807443.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13318..14472)
                     /locus_tag="ACHXOL_000366"
     CDS             complement(13318..14472)
                     /locus_tag="ACHXOL_000366"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR6807444.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14622..15053)
                     /gene="ndk"
                     /locus_tag="ACHXOL_000367"
     CDS             complement(14622..15053)
                     /gene="ndk"
                     /locus_tag="ACHXOL_000367"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR6807445.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15202..17514)
                     /gene="pbpC"
                     /locus_tag="ACHXOL_000368"
     CDS             complement(15202..17514)
                     /gene="pbpC"
                     /locus_tag="ACHXOL_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR6807446.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17515..22465)
                     /gene="yfhM"
                     /locus_tag="ACHXOL_000369"
                     /pseudo
     CDS             complement(17515..22465)
                     /gene="yfhM"
                     /locus_tag="ACHXOL_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
     gene            22672..23517
                     /gene="sseA"
                     /locus_tag="ACHXOL_000370"
     CDS             22672..23517
                     /gene="sseA"
                     /locus_tag="ACHXOL_000370"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR6807447.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23853..23978
                     /gene="timP"
                     /locus_tag="ACHXOL_000371"
     CDS             23853..23978
                     /gene="timP"
                     /locus_tag="ACHXOL_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR6807448.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24010..24786)
                     /gene="sseB"
                     /locus_tag="ACHXOL_000372"
     CDS             complement(24010..24786)
                     /gene="sseB"
                     /locus_tag="ACHXOL_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR6807449.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24928..26211)
                     /gene="pepB"
                     /locus_tag="ACHXOL_000373"
     CDS             complement(24928..26211)
                     /gene="pepB"
                     /locus_tag="ACHXOL_000373"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR6807450.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26389..26589)
                     /gene="iscX"
                     /locus_tag="ACHXOL_000374"
     CDS             complement(26389..26589)
                     /gene="iscX"
                     /locus_tag="ACHXOL_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR6807451.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26601..26936)
                     /gene="fdx"
                     /locus_tag="ACHXOL_000375"
     CDS             complement(26601..26936)
                     /gene="fdx"
                     /locus_tag="ACHXOL_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR6807452.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26938..28788)
                     /gene="hscA"
                     /locus_tag="ACHXOL_000376"
     CDS             complement(26938..28788)
                     /gene="hscA"
                     /locus_tag="ACHXOL_000376"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR6807453.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28805..29320)
                     /gene="hscB"
                     /locus_tag="ACHXOL_000377"
     CDS             complement(28805..29320)
                     /gene="hscB"
                     /locus_tag="ACHXOL_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR6807454.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29416..29739)
                     /gene="iscA"
                     /locus_tag="ACHXOL_000378"
     CDS             complement(29416..29739)
                     /gene="iscA"
                     /locus_tag="ACHXOL_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR6807455.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29756..30142)
                     /gene="iscU"
                     /locus_tag="ACHXOL_000379"
     CDS             complement(29756..30142)
                     /gene="iscU"
                     /locus_tag="ACHXOL_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR6807456.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30170..31384)
                     /gene="iscS"
                     /locus_tag="ACHXOL_000380"
     CDS             complement(30170..31384)
                     /gene="iscS"
                     /locus_tag="ACHXOL_000380"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR6807457.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31625..32113)
                     /gene="iscR"
                     /locus_tag="ACHXOL_000381"
     CDS             complement(31625..32113)
                     /gene="iscR"
                     /locus_tag="ACHXOL_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR6807458.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32414..33151)
                     /gene="trmJ"
                     /locus_tag="ACHXOL_000382"
     CDS             complement(32414..33151)
                     /gene="trmJ"
                     /locus_tag="ACHXOL_000382"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR6807459.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33270..34073
                     /gene="suhB"
                     /locus_tag="ACHXOL_000383"
     CDS             33270..34073
                     /gene="suhB"
                     /locus_tag="ACHXOL_000383"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR6807460.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34218..35072
                     /locus_tag="ACHXOL_000384"
     CDS             34218..35072
                     /locus_tag="ACHXOL_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR6807461.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35263..36543
                     /gene="csiE"
                     /locus_tag="ACHXOL_000385"
     CDS             35263..36543
                     /gene="csiE"
                     /locus_tag="ACHXOL_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR6807462.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36535..37674)
                     /locus_tag="ACHXOL_000386"
     CDS             complement(36535..37674)
                     /locus_tag="ACHXOL_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR6807463.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     SAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37834..38724)
                     /gene="hcaR"
                     /locus_tag="ACHXOL_000387"
     CDS             complement(37834..38724)
                     /gene="hcaR"
                     /locus_tag="ACHXOL_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR6807464.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38860..40221
                     /gene="hcaE"
                     /locus_tag="ACHXOL_000388"
     CDS             38860..40221
                     /gene="hcaE"
                     /locus_tag="ACHXOL_000388"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR6807465.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40218..40736
                     /gene="hcaF"
                     /locus_tag="ACHXOL_000389"
     CDS             40218..40736
                     /gene="hcaF"
                     /locus_tag="ACHXOL_000389"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR6807466.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40736..41056
                     /locus_tag="ACHXOL_000390"
     CDS             40736..41056
                     /locus_tag="ACHXOL_000390"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR6807467.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41053..41865
                     /gene="hcaB"
                     /locus_tag="ACHXOL_000391"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41053..41865
                     /gene="hcaB"
                     /locus_tag="ACHXOL_000391"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR6807468.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41875..43077
                     /gene="hcaD"
                     /locus_tag="ACHXOL_000392"
     CDS             41875..43077
                     /gene="hcaD"
                     /locus_tag="ACHXOL_000392"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR6807469.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        10251 a        11963 c        10877 g        10202 t
ORIGIN      
        1 cttctgcctg tggcaatgtt agcgtcattc actctggcag gatgccagtc aaatgctgac
       61 gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga aactaaaacc
      121 gttaatatta tttccattct tcccgcaaaa gttgccgtag acaactccca aaataaacgg
      181 aacgcacaag ccttcggcgc gcttattggc gcagtcgctg gcggtgttat cggccacaac
      241 gtcggttctg gcagcaattc cggaacgacg gcaggggcag ttggcggcgg agctgtaggc
      301 gcggcagcgg gttctatggt gaatgataaa accttagtgg aaggtgtttc tttaacatat
      361 aaggaaggca ccaaagtgta tacctccacc caggtgggta aagagtgcca gtttacgaca
      421 ggtttagccg ttgttattac cacaacgtat aacgaaacgc gtattcagcc aaatactaaa
      481 tgtcctgaaa agagctaata atcaggagga gtcatgaaga aagtttttct ttgcgccatc
      541 ttagcctcct taagctatcc ggctatcgcc tcatcattgc aggatcaact ctctgctgtc
      601 gcagaagcgg aacagcaagg taaaaatgaa gagcaaaggc agcatgacga atgggtcgcg
      661 gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg caaatgccca ggccgccgct
      721 aacaaaagag cggcaacggc agcggcaaat aagaaagctc gtcaggataa actggacgcc
      781 gaagccactg cggacaaaaa acgcgatcaa agttatgaag atgagctacg cagcttagag
      841 attcagaaac aaaaactgac gctggcgaaa gaagaagccc gcgttaagcg agaaaacgaa
      901 tttatcgatc aggaactgaa gcacaaagct gcgcaaaccg atgtggtgca atctgaagct
      961 gacgccaaca gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt gggcaaagca
     1021 gaagagaaca aatcggacag ctggtttaat taatcgatgt tagtaacttc aagcctatga
     1081 ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt tgttcattca tagtgcaggg
     1141 attaaaatca ttcccactca atggtagctg gcggcttgcc gctgatgtca tacaccacgc
     1201 gggaaatacc gttcacttca ttgataatgc ggttggaaac gcgaccgagg aaatcgtacg
     1261 gcagacgcgc ccagtgtgcg gtcataaagt cgatggtttc gacagcacgc agagagacaa
     1321 cccagtcata cttacgacca tcgcccatta cgccaacgga acgtaccggc aggaacacag
     1381 tgaacgcctg gctgactttg tcgtacaggt ccgctttacg cagttcttca atgaagatgg
     1441 cgtcagcacg gcgcagcagg tcacagtact ctttcttcac ttcacccagc acacgaacgc
     1501 caaggcctgg tcccgggaat gggtgacggt acaacatgtc gtacggcagg cccagctcca
     1561 gaccaatctt acgcacttcg tctttgaaca gctctttcag cggttcaacc aggcccatct
     1621 tcatctcttt cggcaggccg cccacgttgt ggtgagattt gatgacgtgt gctttaccgg
     1681 ttgcagacgc cgcagattcg ataacgtcag ggtagatggt gccctgcgcc agccacttca
     1741 cgtcttccag tttcagcgct tcttcatcga atacttcaac gaaaacgcga ccgatgattt
     1801 tacgttttgc ttccggatcg ttttcgccag ccagcgctga caggaagcga tcttctgccg
     1861 gaacgtgaac aatgttcaga ccaaagtgat cgccaaacat atccagaacc tgctctgctt
     1921 cgttgagacg cagcaggccg ttgtcgacga atacgcaagt caggttttta ccgatagcgc
     1981 ggtgcagtag cattgcggtt acggaggaat ccacgccacc agagaggccg aggatgactt
     2041 tatcgtcgcc tacctgctca cggatgcggg ctacagcatc gtcgataatt ttcgctggcg
     2101 tccacagggc ttcacactgg cagatatcac gcacaaaacg ctccagcatg cgcataccct
     2161 ggcgggtgtg agtcacttcc gggtggaact gtacgccata gaagcgtttt tcttcgttag
     2221 ccataatggc aaacgggcag ctttcggtgc tggctacggt gacgaagtcg gacgggatag
     2281 cggtaacttt atcgccgtgg ctcatccaga catcgagcag cggtttaccg tctgcggtca
     2341 gcgcatcttc gataccgcga accagtgcac tgtcgtttac gacttcaacc tgcgcgtagc
     2401 caaattcacg ttcgttagag gcttcaacgt gaccgcccaa ctgcattgcc atggtctgca
     2461 tgccatagca aacgccgaat accggtacgc ctgcttcaaa gacatactgc ggcgcacgcg
     2521 ggctgttttc ttcagtagta ctttccgggc cgccggaaag aataatgccg cttggattga
     2581 agtcacgaat ttgtgcttct gtcacatccc acgcccacag ttcgcagtaa acacccagct
     2641 cacgcacgcg gcgcgcaacc agttgagtgt actgagaacc gaagtccaga atgaggatgc
     2701 gatgcttatg aatgttttcc gtcattgacg cttattccga ggcaagtgaa acagataata
     2761 taaatcgccc gacatgaagt cgggcgaaga gaatcaggag cccagacggt agttcgggga
     2821 ctctttagta atggtcacgt cgtgaacgtg gctttcctga atgcccgcac cgctgatacg
     2881 tacaaactcc gctttagtac gcagttcgtc gatagtacca cagccggtca gacccataca
     2941 ggagcgcagg ccgcccatct gctggtgaat gatctctttc aggcgacctt tataagctac
     3001 gcgaccttcg ataccttccg gcaccagttt gtcggcagcg ttatcgctct ggaaataacg
     3061 gtcagaggaa cctttggaca tcgcgcccag ggaacccata ccacggtaag atttgtaaga
     3121 acggccctgg tagagttcga tttcacccgg agattcttca gtacccgcca gcatggaacc
     3181 taccatcacc gcgcttgcgc cagcggcgat agctttggcg atgtcgccgg agaagcgaat
     3241 accaccatct gcgataaccg gaataccggt gccttccagg gcttcaactg cgtcagcaac
     3301 ggcagtgatc tgcggaacac ctacgccagt aacgatacga gtcgtacaga tagaaccagg
     3361 gccgataccg actttaaccg cgctgcaacc agcttctgcc aaagcgcgtg cacctgcagc
     3421 tgttgccacg ttgccgccga tgatttgcag atccggatat ttagcacggg tttcacggat
     3481 acgctgcaaa acgccttctg agtgaccgtg ggaggagtcg atcagcagaa cgtcaacgcc
     3541 agcggcaacc agcgcgtcaa cacgctcttc gttacccgca cctgcgccaa ctgcagcacc
     3601 aacacgcaga cggccttgct cgtctttaca ggcgttcggt ttacgttccg ctttctggaa
     3661 gtctttcacg gtgatcatgc cgatcaggtg gaattcgtca tcaaccacca gcgctttttc
     3721 aacgcgtttt tcgtgcattt ttgccagcac cacttcacgg gcttcacctt cacgcacggt
     3781 gaccagacgc tctttcggcg tcatgtaaac gctaaccggc tggttcaggt cggtaacaaa
     3841 acgcacgtca cgaccggtga taatacccac cagttcgttt tcttcggtaa cgaccggata
     3901 gcccgcaaaa ccgttacgct cggtcagttc tttcacttcg cgcagcgtcg tggttggcag
     3961 aacagtctgc ggatcagtca ccacaccaga ttcgtgtttt ttcacacggc gaacttcttc
     4021 tgcctggcgt tcaatggaca tgtttttgtg gataaagccg ataccgcctt cctgagccag
     4081 agcaatagcc aggcgcgctt ccgttacggt atccattgct gcggaaagca tagggatatt
     4141 cagacgaata gttttcgtca gctgggtgct gaggtcagca gtattcggca gaacggtaga
     4201 atgagcagga acgaggagaa cgtcgtcaaa cgtcagagct tctttagcga tacgtagcat
     4261 gggcaatatc tcgaccagag tggttaataa atattgccgc ggcattatac agagcgtaac
     4321 cgattgcatc tacccctttt tgcaaaaaat gcttgctatc cccgaagggc gggttactat
     4381 cgactgaata acctgctgat ttagaatttg atctcgctca catgttacct tctcaatccc
     4441 ctgcaatttt taccgttagt cgcctgaatc aaacggttcg tctgctgctt gagcatgaga
     4501 tgggacaggt ttggatcagc ggcgaaatct ctaatttcac acaaccggct tccggtcact
     4561 ggtactttac actcaaagac gacaccgccc aggtacgctg cgcgatgttc cgcaacagca
     4621 accgccgggt gaccttccgt ccacagcatg gacaacaagt tttagttcgc gccaatatta
     4681 cgctctacga gccgcgcggt gactaccaga taatcgttga gagtatgcag ccggccggtg
     4741 aagggctgct gcaacagaag tacgaacagc tcaaagcgaa gttgcaggct gaaggtttgt
     4801 tcgatcagca atacaaaaaa ccacttccct cccctgcgca ttgcgttggt gtgatcacct
     4861 caaaaaccgg tgctgcgcta catgatattt tgcatgtgtt aaaacgtcgc gatccttctc
     4921 taccggtgat catctacccc accgccgttc agggcgatga cgcaccgggg caaattgttc
     4981 gcgccattga actggcgaat cagcgcaatg agtgcgacgt gttgatcgtt gggcgcggcg
     5041 gcggttcgct ggaagattta tggagtttta acgacgaacg cgtagcgcgg gcgatttttg
     5101 ccagccgcat tccggtcgtc agcgccgtcg ggcatgagac ggatgtgacc attgccgatt
     5161 ttgttgccga tctgcgtgcg ccaacgccgt ctgccgccgc tgaagtagtg agccgtaatc
     5221 agcaagagtt actgcgccag gtgcaatcga cccgtcaacg gctggagatg gcgatggatt
     5281 attatctcgc caaccgcacg cgtcgcttta cgcagatcca tcaccgatta cagcaacagc
     5341 atccgcagct ccggctggca cgccagcaaa ccatgcttga gcgcctgcaa aagcggatga
     5401 gctttgcgct ggaaaatcaa cttaagcgta ccgggcaaca gcagcagcgg ttaacacagc
     5461 ggctgaatca gcaaaatcca cagccgaaga ttcatcgcgc gcaaacgcgc attcagcaac
     5521 tggaatatcg tttagcagaa accctgcgcg tacagcttag cgccacgcgt gaacgtttcg
     5581 gtaatgcagt aacgcacctc gaagccgtaa gcccactgtc aacgctggcg cgtggataca
     5641 gcgttactac tgctactgac ggcaatgtac tgaaaaaagt gaagcaagtt aaagcgggtg
     5701 aaatgctaac cacacgtctg gaagacggct ggatagaaag tgaagttaaa aacatccagc
     5761 cagtaaaaaa atcgcgtaaa aaggtgcatt aagccagcac gaattcaacg cgttttttcg
     5821 atatcaagcc atgcccgtgc tgacagaagt aatctactgc accgcaggct tttaacacct
     5881 gcaacggttg atggcagtct ggacaaagag ctttcatttc aataatttcc ccgcaggatg
     5941 ggcaacgcgc atggccgtta tcctgatcaa gcacatgctg acattgtgga caatgcagtt
     6001 ccatatgaat tcctccggta gccatccctc tacattcata gagggaatgg cagataaaat
     6061 acttacggat aattatttat ttttcttgat gtgcttcatc agacgcttac gtttacgcat
     6121 ctgggttggc gtcagggtgt tacgcttgtt cgcatacggg ttttcccctt ctttgaactg
     6181 aatacgaatc ggcgatccca ttacgtccag cgatttgcgg aagtagttca tcaagtagcg
     6241 cttgtaggaa tcaggcaggt ctttcacctg attaccgtga atcaccacaa tcggcgggtt
     6301 ataaccacca gcgtgggcat atttcagctt cacacgacga ccgcgtacca gcggcggttg
     6361 gtgatcttca acagccatcg tcatgatgcg cgtcagcata gaggtcccca cacgacgggt
     6421 ggagctgtca tacgcttcac gtactgattc aaacaagtta ccaacaccac tgccgtgcaa
     6481 ggcagagata aagtgcacac gagcaaaatc gataaagccc agacggaagt ccagcgtttc
     6541 tttcacctgc tctttcactt cctggctcag gccatcccac ttattcacca caatgacaag
     6601 tgagcgccca ctattgagaa taaagcccag cagcgagaga tcctgatcgg aaataccttc
     6661 gcgcgcatca atcactaaca tcaccacgtt ggtatcttca atggcctgca acgttttgat
     6721 tacggagaat ttctctacag catcggtgat tttgccgcgt ttacgtacgc cagcggtgtc
     6781 aatgagcaca tactcacgtc catcgcgttc cattggaatg tagatactgt cacgcgtcgt
     6841 gccaggcatg tcataaacaa caacgcgctc ttcaccaaga atacggttag tgagtgtaga
     6901 cttacctacg ttcggacgac ccacaatcgc cagtttgatc ggcagacttt gcgggtcgaa
     6961 gtcgtcttcc tcttcttctt cgccgttctc ttccgcttca aattgcgccc agtattcagc
     7021 gtcttcgtcg acttcctctt gcggtgcgag atcttccatc cacggcagca gcacacgctc
     7081 cagcagactt aatacgccac gaccgtgaga tgcggcgatc gggtagattt cacctaaacc
     7141 aagcgagtag aaatcaacca ctgcctgatc gggatccaga ccgtcagttt tgtttgccac
     7201 caggaaggtc ggtttttcac gagagcgcag atgtttggca atcgcttcat ctgccggcat
     7261 caagcccgcg cgcgcatcca ccataaacag tacgacgtcc gcttcttcaa tcgccagcag
     7321 cgactgttcc gccatgcggg tttctacacc gtcttctgtg ccatcaatcc cgccggtatc
     7381 aatacagata aactcacggc cttcaatttc cgcacgaccg tacttacggt cacgagtcag
     7441 acccgggaaa tccgcaacca gcgcatctcg ggtgcgagtt agacggttaa ataacgtgga
     7501 ttttcctacg ttagggcgcc cgacaagcgc gaccacaggt accatgttta aagcctcatt
     7561 tttataaatc atcagacaac gcacgctata ttcgcgtcgt tgttaaaaac aggaaaacgg
     7621 cccctgtcca ggagccgttt tcaaagtgaa cgacagagac gattaacgtg taatagagta
     7681 cacggttccg tcttttgcct ggatcagcag tttgccgtca gcggcaaccg gttcagtctg
     7741 gaaaccggaa ctatcaactt tttgctgggc aacgaaacga ccatcttcga cgttaatcca
     7801 gtgcagataa ccttcactgt cacctaccac caggttgcca ttatacagca ccggcgaagt
     7861 cagcaggcga tgcagcagat cgctttgtgt ccacagcgta acgccgccat caatggtcaa
     7921 cgccatcacc cggtcatttt gatcgaccag atagatgcga ttgccgtcga cgatgaaatc
     7981 attcaccgaa cccagttcgc gtttccacat aatctgacca ctgcgcagat caagcgccgt
     8041 caggttacca ttataggcca gagcgaaaac aacgccgtta acaacgacgg gagtcgtgtc
     8101 aacatcgctc agacggtcaa tttcggtaga accggtcgcc tgggaaatac gctgctgcca
     8161 aatcatctgg ccctgttcca tcagcactgc gctgacgcga ccattatcgc cccccacgac
     8221 ggccgcacca aaagccgttg ccggcgcaga ctcgccacgc aaagagagcg aaggcatatc
     8281 gaggttaact gtccatttga cagcgccgtc agcttcgttc agcgcttgta actgaccgtt
     8341 actagtgtgg attaacacca gaccgtcgct gaccaccggg cgagaaagtg cttcacccgc
     8401 gactttagtt tgccatgcca cagtaccatc gctggtattc agcgcgtaaa cctgcgcctt
     8461 ttcgctgcca atgtagacat gcccaccaga cacggtcaca ccgccagaaa gtaatgcagg
     8521 ctctttagag aaccagccat ctttctcggc caggctgaca gaccagattt ctttgccatc
     8581 atccgcattc agcgctttta ccaaaccagc gcggtccgct gcatagacaa cgttgtccgc
     8641 cagtgccgga tgaagattgg aatagaagtt gccaatgccg ctaccaacgg aagtgctcca
     8701 cgccgtggtc ggcgtaaact ggttttcaac ggttggcaat ggggacatct ttaccacatc
     8761 ttcttcgctg ttaaacagcg aacagccgct taaaagggta acggaaagca gtcctggcag
     8821 cagtaattta cgcaattgca tcgggtccct ctcagatgga caaattatta attttcatct
     8881 gcatcatttc gctcagtgcc ggagtaacat cgcttttcac gcctgcttcc catgcactac
     8941 gcgcaccttg cttatcacct ttgctcaaca atgctttacc acgcaggtcg gcaacaatgg
     9001 cagcccaccc ttcacctttg atggtatcaa gggttttcag cgcggcatca gcctgcttga
     9061 gctgtacctg aacgcgagca agacgcagat ttatcacggc tttgagattt tcatcactcg
     9121 tgtctgccag cccctgttgt aactgggcgg cagctttctc tagttcattt ttgtcaacaa
     9181 attgctgcgc aagttccaaa gaagccagcg caccataagt atttttattt tcagcagcaa
     9241 atttttccgc cgccgggatg ctatccggtt tgccttcgct cactgcggta accgcatttt
     9301 gataggcaag agaagcggag cgtgcagaat caacctgatg gctgttccag tagcgccagc
     9361 caatcagtgc gccaacgccc aaaatcaccc caacagccag tgctttgcca ttttcagcaa
     9421 aaaagcgttt aaccgcttct acctggtcgt tttcgttctc gtaaatttcc acgctgtcct
     9481 tctccttcct taacccagta acgtgcgcaa atgcgcggct acgctatcct gcgcaactgc
     9541 cgtttgctca ccagagcgca aatccttcac tactgctgtg ccgttagcca cttcagactc
     9601 acccagcacc acagcaacgc gggcacccca tttatcagca cgggcaaact gtttcttaaa
     9661 gttgccgccg ccgtggttgg tcatcaattt cacgcccggt aattcatcac gcagacgctc
     9721 agctaatgcc atagccgcag attgtgtatc agcacctgaa gccaccaggt atatatcgac
     9781 aacaggatcg gctttaaatt ccggattaac ggcctgtact aacaatacaa gacgttcgag
     9841 gcccatcgca aaaccgactg ccggtgttgc acgaccgccc agttgttcca ccagaccgtc
     9901 ataacgaccg cctgcacaca cggtgccctg ggagccgagg ctgttagtca cccactcgaa
     9961 aacggtacgg ttatagtaat ccagaccacg caccagacgc tggtttacgg tgtaagcgat
    10021 ccccgcgctc tcaagcagtt tgcacagacc ggcaaaatgc tcacgagatt cctcgtccag
    10081 ataatcacct aatgccggag cgtcgttgag aagcgcctgc acttccggat tttttgaatc
    10141 cagcacgcgc agcgggttag tgtacatgcg gcgtttgcag tcttcgtcca gcttttcttt
    10201 atgctgctca aggaatgcca ccagcgcatc gcggtaattg gcgcgtgctt ccagcgaacc
    10261 aatagagttc agctcaagag ttacgtgttc gaagataccc agcgcacgcc accagcgggc
    10321 agtgagcata atcagttcag cgtcgatatc cggaccttgc agaccgaaaa cttcgcagcc
    10381 caactgatgg aactgacgat aacgcccttt ctgcggacgc tcgtgacgga acatcggccc
    10441 gatataccac agacgctgtt cctgattgta cagaagacca tgctcgatgc cggcgcgtac
    10501 acagcccgcc gtcccttcag ggcgcagagt caggctgtcg ccattgcgat cctcaaaggt
    10561 gtacatctct ttttcaacca cgtcggtgac ttcaccaatc gcacgtttga atagcggggt
    10621 ctgctctaca atcggcaagc ggatttcact gtaaccgtag ctgccgagca cgtttttcag
    10681 tgtgccttca atgcgctgcc agatggccgt ttcgccaggc aggtaatcgt tcatgccgcg
    10741 aatggcttga atgttttttg ccacgtttat tctctttctg aatataaaaa tgaaccctca
    10801 acgcttccct caatgtttcg ggagccatgc gggttcaatc atacacggga agcgaggcgc
    10861 ttcccatcac gttattattt ttcaacctgc tgaacgtcaa ttcgacgcgc ttcgtccagc
    10921 tgactggctt tcgcacgaat gcgtgcttcc agctggtcga tcatatcgtt gttgtccaga
    10981 cggtctttgc gcacgccatc ttcatagagg ccgcttttct tgttgccgcc ggtgacgccg
    11041 agtgtagaaa ccagcgcctc acctgggcca ttcaccacgc agccgataat cgaaacgtcc
    11101 atcggagtga tgatatcttc caggcgttgc tccagcgcgt taaccgtacc gataacatca
    11161 aattcctgac gcgaacaggt cgggcaggcg atgaagttaa tccctcgcga acggatacgc
    11221 agcgatttca aaatatcgaa accgactttg atctcttcga ccggatcggc cgccagcgat
    11281 acgcgcagcg tgtcgccgat gccttccgac agcagcagac ctaaaccaat ggcggatttt
    11341 actgccccgc tgcgtgcacc accggcttcg gtgatcccca gatgcagcgg ctgatcgatc
    11401 tgttttgcca gcaaacgata agactcaaca gcgaggaaga cgtcagacgc tttcacgctg
    11461 actttgaact gatcgaagtt caggcgatcg agatgatcaa catgacgcat ggcggattcc
    11521 agcaacgcct gcggcgtcgg ttcgccatac ttttcttgca gatctttttc cagcgatccg
    11581 gcgttaacgc caatacggat cggaatgttt ttatcgcgcg cacagtcaac caccatgcga
    11641 atacgctctt cattaccgat attgccaggg ttaatacgca gacaatcgac gccgtattcc
    11701 gctactttca gcgcaatgcg atagtcgaag tggatgtcag ccaccagcgg cacgttaacc
    11761 cgctgtttga tgagtttgaa cgcttctgcc gcgtccatcg tcggtacgga gacacggacg
    11821 atatcagcgc caacgcgttc cagcgccttg atttgattga ccgttgcttc gacatctgtc
    11881 gtacgcgtat tggtcatgga ctgtacggcg atgggagcac catcgccaat cggcacattc
    11941 ccaacgtaaa tacgtgttga ttttctacgt tgaattggag cctggttatg catgaaaaat
    12001 ctcccgcgtt acccgtctgt tactgcgccg gtgattgttc ggcattgagg gtcagacgcg
    12061 caacctggtt agttctgata aaacgactca gatcgacagg tttcccttga tactggatct
    12121 gtactgcggc tggcgcacca attttcagtt tatacggtgc ctggccggtt aaattcaaat
    12181 taccgtcttt acgctgcatc ccgctaaaca attttttacc ggtagcatca gtgacctcca
    12241 gccagcaatc ggcagtaaag ttcatcacca gcgcattcgg atcagccgcc ggcgtggtca
    12301 cgccagcctg atcggttggc aacggcgcag caccatctgg cgttgttgct gccgttggta
    12361 ccggggtcgc ggcggtatca acatttgcct gcgaaggcga aacaaccgca ttctgttgcg
    12421 gatcaacagc tggtgctggc gcagttacgg caggtgtttg cgtgttggtt gcggtagtat
    12481 ccacagacgc tggcggcgtg gacgttgtag ccgggtctgt agttgtcgac gtatttaacg
    12541 ggacactctg cccctgctcg ctattactgc tcagttctgc cgaagattga tcggccatag
    12601 tggtgatctc ttcctgctga gctttgtggt cttgccacca ccaggcaccg ctcaggccga
    12661 taaccacaaa caacaccagc caggtgaagg tcatcagcca gccatcacgt tttttgcggc
    12721 gtttaccgag ggaaaaactc tgcattggcg caacttttgc agcccgtagt ggagcctgct
    12781 tttccagccc tggcagcagt tcttcttctg gaatatgtac cagacgcgca taagagcgga
    12841 tatatccgcg caggaatgtt gaagcaagat cggcgggtgc cttatcttct tcaatgtcgc
    12901 gtaccgtgga aaccttcagg caaagtcgct cggcaacggc ctgctgacta agtcctagtt
    12961 gttcgcgagc attacgcagg cgagcgccgg tagtaagtgc ttcattttgg tcgtgcgtgg
    13021 cttcagtatt cattcgctgc aggtacgttt aaatgagaat tgagatgccg gtgaaccatc
    13081 atgctcaccc acaccgcgaa acatccgtta agttaaccgt tatcatacag tataagaccg
    13141 tctgtgtgct cgtgacaaag cccacattca ctaaggctaa cttactgttg catcgttaca
    13201 tactgcctta aagtcagcaa aaacgcaccg ttaatattga ccagacaatt gcaacttaat
    13261 acttcattca taaaacatta cgccacggta cataaagtaa ccgtggcgta atggctatca
    13321 gaccgcttta atgtcgatgg cttcaccctg catccgttta cgcagggtac gtttcgtacg
    13381 gtcgataaca tcgcccgcca actgaccaca ggcagcatca atatcatcac cacgagtttt
    13441 acgcacaata gtggtgaaac cgtagctcat cagcactttt gagaaacggt cgatacggct
    13501 gttcgagctg cgtccatacg gcgcacccgg gaacgggttc cacgggatca ggttgatctt
    13561 acacggcgta tctttcagca gttccgccag ttggtgcgcg tgttcagtgc cgtcgttaac
    13621 gtggtcaagc atcacgtatt caatagtgac tcggccctga ttggcgttgg atttctccag
    13681 ataacggcgc accgcagcaa ggaacgtttc gatattgtac tttttgttga tcggcacaat
    13741 ttcgtcacga atttcgtcgt tcggcgcgtg cagggaaatt gccagtgcaa cgtcgatcat
    13801 atcgcccagt ttatccagcg ccggaactac accggaagta gaaagcgtga cgcgacgttt
    13861 agacaggcca aaaccgaaat catcaagcat gatttccatc gccggaacga cgttgttcag
    13921 gttgagcagc ggctcgccca tgcccatcat cactacgtta gtgattggcc gctgaccggt
    13981 gacttttgct gcgccgacga ttttcgccgc ccgccacacc tggccgataa tttccgacac
    14041 ccgcaggttg cggttaaagc cctgctgggc ggtggaacag aatttacact ccagcgcaca
    14101 ccccacctgc gaagagacgc agagcgtggc acggtcgtct tccgggatat acaccgtttc
    14161 gacgcgctga tcgccaacgg cgatcgccca tttaatggtg ccgtcggatg aacgctgttc
    14221 ttcaaccact tccggtgcgc ggatctccgc cacctctttc agtttgccgc gcaacacttt
    14281 gttgatgtcg gtcatctcat caaagttgtc gcagcaatag tgatacatcc acttcatcac
    14341 ctgatcggcg cggaagggtt tttcacctaa atctttaaaa aactcccgca tctgctgacg
    14401 gttgagatcc agcaggttga tttttccatc tttcgtggtg acgttttcag gtgtgactaa
    14461 ttgttcagac atatgctatt ccggcctcgt tattacacgt tatggcccct ggagggttga
    14521 aaaaagaaac gccccggtga gcggctgctc gtccgggggc gctgcattgt acaaattctg
    14581 gcgcacggat gccacgtttg cacgcggcat ttacgaaatt attaacgggt gcgcgggcac
    14641 acttcgcctt cgccgaagaa ataagcgatt tcgcgagcgg cagattcgac ggaatcagaa
    14701 ccgtgggtac cgttttcggt caggctgtca gcgtaatcag cgcgcagagt accggccagt
    14761 gcgtttgccg gattggtcgc gcccagcaga tcgcggtgac gctgaacggc gttttcacct
    14821 tccagcacgg aaaccacgat cgggccagag gtcatgaatt caaccagacc atcaaagaac
    14881 ggttttccat cgtgttcagc ataaaagcca cgagcctgtt caacggtcag gtgcagcatt
    14941 ttggtgccga caattttgaa ccctgcagct tcaaagcgcg caaagatatt accaatgacg
    15001 ttttttgcta ccgcgttcgg tttgatgatg gaaaaagtac gttcaatagc catttttacc
    15061 tctgtaaatt gttctgttgt tgtctgtacc agcgtacgag atggcgcgga ttataatgag
    15121 caacagggcc gttgactatt gatgaaggta acattttttt aaaataaaat gagttttagc
    15181 aacaatcagg gacaaaacag actattgcat gacaaatttc actgtcgcga tttgccccac
    15241 gtcatccatc accagcaatt gataatcacc tttatccgtc aaatgcaggg tcacgttgcg
    15301 cccgcgttca gttaacggtt cgccattcag aaaccaccag cgttcacctg cccctccact
    15361 tgattgcaac ggcaaagtcg cttccgctgc gcccggtaaa cgtttaataa tcgcgccatc
    15421 gcgaacgcct gtcagctgca gagggagctg ggcatcgtga ccgtatggcg ggcaaattgt
    15481 cgaggccggt ggtaagcgca cagcgcgacg ttctgatgcg ggcagccagg gttccagcgg
    15541 tagcggccag acatttacca tttcttgctt cgcctgcggg cagtcagcgg cgacgcgctg
    15601 ccctttgtca tccagccaga tggggaagcg aatgccatta atcccttcct gctctgacag
    15661 taacagcgtt ggcggctggc tgccatccag cagccaggtt gccaggcggc ggcgacagtt
    15721 accgtcacct tccggcaaag actgtccgcc cggccagcag ataacgccac gagtgactga
    15781 gttcgggcgc gggtcttccg gcagattcgc actgcgcgac agtaagatat tattgacctg
    15841 attcaacaat ggtacggcac tggcaaagcc aaactgacca acaacgggcg tgccgtcagg
    15901 tctgccagtc cagatcccaa tgacatagcg agcgttaacc ccaatcgccc aggcgtcacg
    15961 atagccatag ctggtgcccg ttttccatgc cagtggggcg acgcgcggca aggcactatc
    16021 cggcaagggt tgcgcttcat cagccataat ccggcgaatg atccacgccg cccccgacga
    16081 cattaaaggc cgttcaagca gcggatcgtc aggctgtaag cgcaatttgc ctgccttgcc
    16141 gtggcgagca aacgcggtat acgctgccgc catatcttcc agttttgcac cagcgccgcc
    16201 gagaatgagt gaaagattcg gcgcagcgcc gttgggcaaa tataacggca atccaacatt
    16261 gcgtaacttt gccgcaaacc gtttcggtcc ataggcttcc agcacctgca cagcaggtaa
    16321 gttcagcgag cgcaccagcg cctcgctcat gctgatcggg ccatgaaaac cgctatcaaa
    16381 gttacctggt cgataatcac cggtgcgccg ggggacgtct tgcagcagtg atgccgggtg
    16441 gatcaagcct tcatccagcg ccagaccata aacaaacggt ttgagcaccg atcctggcga
    16501 tcggatcgca ttgaccatat caacatgacc aaagcgtgaa tcatcgttga gatcaaccga
    16561 tcccacccag ccgcgaacac gcatatcggt atgatcaacc acgatcatcg ccagtgagct
    16621 gcgcggtggc aaccgccctt tccagttttg cgccagttct tccagacgtc gttgaagacc
    16681 ggcatccagc gtagtaacga ttttgtcgct tttgctttta ccgagcatca tgcgcgaaaa
    16741 cagcggtgcc agttgcggca tttgtcgggg tgccagccag atgggttctt cccttgactc
    16801 ttttacctgc tcacgggacc acacaccttg cgcagccatc cgttcgagca ctttattacg
    16861 cgcggcttcg gcacgctccg gccaacgatc cgggcgaaga cggctgggcg cttgcggcaa
    16921 aaccgccagc attgccgcct cggaatagct taaattcgca ggcgactttc cgagataagc
    16981 ccaacttgcc gcaccgatcc cctgcaacgt accgccaaac ggagcgcggt taagatacaa
    17041 ggtcagaatt tcacgcttag acagatgcca ttccagttgc aacgcgcgcc agagctggcg
    17101 aattttgccg ccaaatgttt tggggtgagg atcaagcaga cgagcaacct gcatagtgag
    17161 cgtgctgcca ccggaaataa cccgtcccga agtgagatct tgccatgctg cgcgcgccac
    17221 cgagaatgga ttcacccccg gatgcttcca gaaccagcga tcttcataat tgatcagcgc
    17281 ttcaaggtaa cgtggagaaa catcttcgat tgttaccgga taacgccaga tgccgtcagc
    17341 atcggcgaag cgccagagcg gcgtaccatc ctgcgccacg accactcgtg cgggattgac
    17401 ttcatgcagc ggtagaggcc agagtttatc tgctccccac gctgcaagaa aaaggagaaa
    17461 tggcgcagct gccagcgtta tccagcagcc gcgtttggtt aacaagcgag gcatttacgg
    17521 tcggacaatc agcagatctt cagccgcggc ggtcgcccgc cattggggaa catacattga
    17581 ttccaccatc ggttgcggta cctgatacgt tcccggcgtc accgcccgcg ccagatacac
    17641 cagcgttacc ggttggtatt catcaacggc aaccgccgcc acaaagcgat cgtcacggaa
    17701 ctcaatgtgc ttaatactcg cctgctgcat ctggttcagt aagttctgca cttcgccacc
    17761 actttgctcc aggctggcgc taccgttcgc cagattctgg ttttccagtt ccagacccac
    17821 aggcagcaga tccacgacta acgcatccgg cacgctgtta ctggctttta cctgcaacca
    17881 caccagcacc agatcgccgc tacgtaacga gtccagcgat ttgctcttac catcagtacc
    17941 aagaatatga cgctcgattt gcagcacatt gttcgccggt aaaggtgcgg attgcggata
    18001 accgctggca tccatacgca accataacgg ctgatcgcca ctgttgctca cctgcaaggt
    18061 gacaagttga tcgctattca gattgctgtt ttgcgctttc tcgcctgtca gcggctcagc
    18121 tgagaaagag gtttgcgcct gccatttacc cggtaaatcc tgaatcgtac gggcagccag
    18181 gaacaacgcg ttactttcct gcgtcgatag ccagcgttca ccaaacgcct gctgcgaaag
    18241 tgtgttcagc aaagtgtact gctcatcggg tagcagttta ttttcttcca gcaaggagag
    18301 cattaacgcg ttgtcgcgca gtggactacc gtaatcaccc agccatatcc gctcatcact
    18361 atttctcggc gttttcagcg ccagcgcaat cgcttcttca ccacgcgtcg catcacccat
    18421 ggttttcagc gcaatgccaa gttgcagcag cggtaaacca gaagcggcat ctgcacgatg
    18481 ctcccagatt tcacgcagcg cacccagtgg tgctttttgc tgacgggcca gcaccagcgc
    18541 agcgtaagac tgaacggcga atttactggc tttgagatta tccgcgtacg ggatcgacat
    18601 catgcccgga tcttgtaaat agcgcagcag acgctcatta ccccggttaa tggcgtctgt
    18661 cggcacgctg taaccctgtt cgcctgcgcg gaccaggaaa tccatcacgt aagccgtcag
    18721 ccagtactct tcgtcaccgt ttttatccca cagcgcaaag ccgccgttat cacgttgcat
    18781 ttgcagcaaa cgggaaatgc cgatatcgac cgatgcacgg cgtttctcat cactgtcgcc
    18841 tttgatgccc aacgcctgca gttgggctgc gttggtataa agtgacggga acaggccgct
    18901 ggcggtttgc tcaagacagc cgtacggata cgcttttaac tctttgatat aacgtgcaat
    18961 gttcagcggt ggtttgccgc tcaacaacaa ttgcccttcc agcgtaacag gcgagaagtt
    19021 ttgcaatcca tccgccggaa tcgcccatgt ctcaccaggc tgtaacgccg taccgtaatt
    19081 aaccgtttgt gccgggaacg ccggacggac gccgattttc cactgcttat gctgatcggc
    19141 aacggtttca cccggtaacg ctaacccgct aatggtggcc tgaatttctc catcgccata
    19201 acccggcaat gctcgcaccg ggataaacag cgtagtacgc acacctggcg ctaattcaac
    19261 ggctgcgggt gaatcgctga ccagttcaag caaaccactg gcggtcaggg caacgttcag
    19321 tttttgcggt ttatcggtaa gattagtgat atccagcgtc agacgcgagg tatcgccact
    19381 cgccataaag cgcggcatgt tcagttcagc aatcaccggt gcggcaacta tcactttgct
    19441 ttcgttgcta ccgaaatcat ctgccgtcca ggcttgcgcc atgacgcgca attcaccgtt
    19501 aaaatcgcca atcggcagtg taaccgagcc ttcgccctgt tcgttgagcg ttaccgacag
    19561 cgcctgctgc gcgacaatat tgacgtgatt gaccggcggt ttaccaccac gtttcagctc
    19621 atcaccatcg ccaccgaaac gcagagctgc cagacgcccc tgaccttcaa taacctgacc
    19681 gtaaatatcg taaatgtctg cgccatagcg tttctgacca aagaacgcct gccacggatc
    19741 tggcgtaacg tagtcagtaa tattcagcac accactatca acggcagaca ccagcacatt
    19801 cacctgttta ggcttctcgc catttttagt gctggcttta attttcacgg ttaatggttg
    19861 attgggacgc atttttgctg gtgtttccag cgccagatcg aggcgacggt tttcatcacc
    19921 aagcggcaga tgcaacacac caaccgcgcg ttttggcgtc gcggagcgag atttatcgcc
    19981 aggacgcacc accagcgtac tcaaataaag atcatgacga ttccaggttt tatcgaccgg
    20041 aatcgtcaga tccagccctt gagccggaac atcaatctct tgccaccaca gcggcccttc
    20101 actggactcg accatcgcat aacctttacc cgccgttggc gcggcaatat gcaacttaat
    20161 ggtatcgccg gggcgataac tggctttatc tagtttcagc gtgacacggt cgggtcgcac
    20221 tgcgccgctc ccgtcgctgt tgtcctgcca gctatagcca gcccaaaaac gcacgctact
    20281 gaccgcttca ttcggcgctt tgacttccag acgataagcg ccccactcta ccgggaaact
    20341 gaccttaccg gtttcatccg cctgaagatc cagagtttgt tcattttcga tcagatcttt
    20401 ttgatcaaac tgtgactgcc agccttcatc ttctgaccag ttccagtagt aatcgcggcg
    20461 ttcgcgaatc aggcgcacct gcaagcccga cacggctttt ttcacgcctt gcgcatcgct
    20521 ataaacgatg tcaaaagcgg cgttactgcc ttcatcaaca atgggctgtt tcaccgtgct
    20581 gtcagtgcga taatcgtaaa ccgatttcaa ggcgaactgc ggacggagcc ccggcaatgc
    20641 atcgagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc cgattccagc aaactgccct
    20701 ggaaaataac ctgcaatggg gaatgcgttt ccttccactg gctttctgta gaaacttcgc
    20761 cgcgcccttt atcatccagc gtcaactgaa cttcatccag cgtgcgggaa aggttttcgg
    20821 cagctatatc gccgaattca aaaccaggta aggctgacac agcctcacgc agtgggcgca
    20881 ggaaaagttg cccttgcaaa gtattaccat tagcaggcgc accgtacagg tagtatccca
    20941 ccacggagaa tttcacttca tcattcggcg taagcggggt tttctcaccg gtcagattca
    21001 gcgccatacg ctctggcata aaatcttcga cgtggaaatc ccacatccgg tactggttat
    21061 cgcccgtgtt ggcgcgaata tgccacatac cggttgccgc attgctatcg agtggccagg
    21121 taaagtggta gaggccattc tccggctgac taacgacgtt cctgagtacc tgcccgtcag
    21181 gtttaatcac gtctaacttg atgggttgat cgggcaatgc tttaccgtct gcatcgcgca
    21241 gtaaaccatt gaggattacc gtttcacctg ggcgataaag atcgcgtggg ccaaacatga
    21301 aaaactgttt gctatagcct ggcgcgccag caatgttaaa ttctgctaag tccagcgccg
    21361 gaagttttaa atcgagtagc gttgtctgac cgtctttacg tgccagcagt aatgccgcat
    21421 ttttatcatt ttccagctgc acatgcccct gagcgtcact ggttgcctga gtcagagtct
    21481 gtcctttctc atttaataaa gagacttcaa ttccttgctg ggccgcgccg ttttccagac
    21541 tttgggtaaa gatatccaga cgattgtgat aacggtgagc tgaaacgccg atatcactta
    21601 acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc agcctgattc atcacagcca
    21661 gatacacgcc cgcctgctga agcggtttga tatcgcccag cggcagcaat aatttttcac
    21721 gggtgttacg cgcaggattg agatcaaacc gtccggtgta gaccagatcc gccatctgca
    21781 gcagtttgtc tgactgccag ttcgccagcg aattgcggta ttcccattgg ctaatgaatg
    21841 ctggcagaga ttctggctta acgcggaaga agttaacatc gacattatta acgttgagcg
    21901 ccattaccgg cagcccttca acgactttgc caggcagcag cgaaccacgg ctggcaaaac
    21961 cgacgctggg ttggatgtcg cgggtagtta tagttttttc gtaatcttta ctgaaggttg
    22021 cgttgttgag tgctttgacc tccttgccaa tagtaacgat caaatcacgt ttcggttcga
    22081 ggtggcgtaa acgtagctct ttaagattat ctgacagctc ccaggcacca tccactttgc
    22141 cgcttttttt atcgacgaca tgaataacgc gtgagaaatc ctgatccggg tcgagaggga
    22201 tggagaacgt cagcaccagc gtggctgcac catcaagttg gacttcagag agatccagca
    22261 atgtcagcac cttaccggca ctctgttggg ccagtttttg tctctccggc acggagagtt
    22321 ttgctgaact cgcgttttct gaagaggccg ctttagtaac ttcagaaggc gcatcttttt
    22381 tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc cagcgccagc attagcatgc
    22441 aggcggctac gcgtaacttt ttcatttttc atccctgcca caatggcccg ttagcaacgt
    22501 cgaataatta ttatgcgtga gaatttacgt tatgtaattc agtatcacgc ccatacaatc
    22561 cgaattttag tgagaattgt ggctcagaca gccaaatcag cctctttcgc ttgtcgcttt
    22621 tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa tggagatgcc tatgtccacg
    22681 acatggtttg taggagccga ctggctcgcc gaacatattg atgacccgga aattcagatt
    22741 atcgatgccc gcatggcgtc gcctggacag gaagatcgta acgttgctca ggagtatctg
    22801 aatggacata ttcccggcgc agtgtttttt gatatcgaag cgctttctga tcacacttcc
    22861 ccgcttccgc acatgctgcc gcgcccggaa acgttcgccg tggcgatgcg tgaattaggc
    22921 gttaaccagg ataagcacct gattgtctat gacgaaggta atcttttctc agccccacga
    22981 gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt cgattctggg gggtggactt
    23041 gcaggctggc agcgcgatga tctgctgtta gaagaaggtg cagtagagct gccggaagga
    23101 gagtttaacg ccgcgtttaa tcctgaagcc gtggtgaaag taaccgatgt attattggca
    23161 agccatgaaa atacggcgca aattattgat gcccgcccgg ctgcacgttt taacgcagaa
    23221 gttgatgaac ctcgcccagg tttacgtcgc ggacatattc ccggagcact gaatgttccg
    23281 tggacggaac tggtgcgcga aggcgaacta aaaacgaccg atgaactgga tgcgatattt
    23341 tttggtcgcg gcgtcagcta cgacaaacca attatcgtca gctgcggctc tggtgtaacg
    23401 gcagccgtgg ttttgttagc actcgcgacg ctggatgtgc caaacgtgaa actgtacgac
    23461 ggcgcatgga gtgaatgggg cgcgcgggca gatttaccgg ttgagccagt gaaataagta
    23521 ttttacaggc aataaaaaac cgccgaattt ggcggttttt tattgctggt ctggttcgcg
    23581 gcctttccag caggttgtat taccgtagta atgcaagcgc gtctcagcgg agacaatact
    23641 cgccagtaac tctctttttg tcaagcaaaa gagagttatt attgttctgt tagtgtatta
    23701 tccactgcgg ccctttccgc cgtctcgcaa gcgggcgctg gcttaaggaa aggatgttcc
    23761 gcgaccgtta gagcaagcgc gtctcagcgc ttgctatcgc ggcaatatcg ccagtggtgc
    23821 tgtcgtgatg cggtcttcgc atggaccgca caatgaagat acggtgcttc tgtatcgtac
    23881 ttattgtttc tggtgcgctg ttttcccaag taaataataa ccggagtctc tccggcgaca
    23941 atttactggt ggttaacaac cttcagagca gcaagtaagc ccgaatgccg ccctttaggg
    24001 cggcatattt tagattatcc ggttttgttt aaagtcacgc aaaaaaccac cccagcgacg
    24061 ttcatagaat ggcacaatat gttcggtaat aaagtggcta attccttttt cccctttttt
    24121 cacctgacaa atatcgattg gttcatcgcc aggtaatgta tcggtcgcta cacttcccgt
    24181 cgcctgaata atttcttcga tatcaccatc ggcttcaatg ccaataagta aattaggctg
    24241 tgcctcttcg ttctctttaa ttgaacaaat aaaagcacgc ttcaccggct taatggtttt
    24301 aaataaggtg gtgagtgaat caatcatttg tgctggcggc tctgcgactt ccgataatat
    24361 cagcgattca ccgccttcca ggatttcctg gctgctcagc ggatttccct cttcaccaat
    24421 caacaaactg atttcacgcg gcataaattc tttaccggtc ggcagtttgg cattaaggaa
    24481 gagcgtttcg ccaagggtca tctcaaacaa tgtgcgaacg ggcattacga caaatgcctg
    24541 ttcgtcttca accgcctgtt gaagcgcttc taacgaggtg aaaaaaggaa tgacgctggt
    24601 gccgtcttct ttttcccagt gctgtaaatc aagcgcacta tcttcaacca cagcctcgcc
    24661 ctgcgccgcc gtaccaggca cccagacggt ggattccagt agagtacgga aaaaggccgg
    24721 gcggtgcgcc ggttcggttg ctgctttttc cagcaggtct tcaagttcgt tttttgtttc
    24781 ggacataaga accacaattc attcaacgtt cggggcgcaa atgcctgatg cgctacgctt
    24841 atcaggccta caagggattc gcaatttgtt gaatttgcag aatttgtagg ccggataagg
    24901 cgtttacgcc gcatccggca tattagttta cgccgttaac agattagcta tcgtgcgcac
    24961 accaagtccc gtagcgcccg cagaccactg ttcaaccggc gctttacggt aagtcgccga
    25021 gcagtcgata tgcagccagc cttgctgata gttctcaaca aagtgcgaca ggaagcccgc
    25081 cgccgtgctc gcgcctgccg gatacgccgc gcttccggta ttgttcagtt cggcaaagtt
    25141 agacggcagc tggctgcggt ggaattccgc cagcggcaga cgccagaatg gttcgttctc
    25201 ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg tcaaaactga acagcgcgtg
    25261 ataatcatta cccagcgcag ttttcgccgc cccggtgagg gtcgccgcat caatgatcat
    25321 ttccggtttc tgcgcactgg catcaatcag accatcggca agcaccagac gcccttcggc
    25381 atcagtgttc atcacttcaa cttttttacc gttgcgatag gtgatgatat cgcccagctt
    25441 gaacgcattg ccgctaatca ggttatccgc acagcagagg aacagcttca cgcgcttgtt
    25501 cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc gttgccgcgc cgcccatgtc
    25561 cgacttcatc gagtccataa acgcagtctg tttgatgctg tagccgccgg agtcaaaagt
    25621 gataccttta cctaccaggc acgcgtacac tggcgcttct ttatcgccag ttgggttgta
    25681 atccagcgcc agcaataccg gagaacgttc tgaaccgcgt ccgacggtgt gcagccccat
    25741 ataaccttgc tcacgcagat cttcgccttt ggtgatccga taagtcacac gatcgcccgc
    25801 gacgttgctg atcagatcaa cagcacgctg tgccagttgc gatggtccca attcttctgc
    25861 cggtgcgttg atggtgtcac gcacccagtc gatgatcatc aggcggttat ccagttcctg
    25921 gcgctgggca tcgtccagat ccggccactc cactttacgc gtgcctttcg gggctttgta
    25981 accttgccag aatgcccagc agcgatccgc atcccagcct tcaccgctta actgaacatg
    26041 cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg atcagcccca gatcgtctgc
    26101 cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg taagttgctt tttctcccca
    26161 gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc gcttctgtca ttttagttat
    26221 ccttctttgt aaaagggccg ccagttggca gccccaggat ttttccgcta aatgattcgc
    26281 gttgcagaaa ggcggcaagt gagtgaagcc ccaggagcat agataactat gtgactgggg
    26341 tgaacgagcg cagccaacgc atctgcggcg tgaagcatga cgcggaaatt attcggcctc
    26401 gtccagccag actaacaaaa tcgcttcgag gattttctcg ttggatgcct gcgggtcgtc
    26461 gtcgaaatct tccagatcgc aaatccactg atgcatatcg gtgaatcgaa ccgttttcgg
    26521 atcaagatca ggatacgcat cgtacagtgc ttcgccaatt tcgcggctat cggtccactt
    26581 aagtcccata ctaacctctg ttaatgctca cgcgcatggt tgatagtgta acgcgggatt
    26641 tcgactacta aatcttcgtc ggtaacgcgt gcctggcagc ttaaacggct ttcgggctcc
    26701 agtccccagg ctttgtccag catgtcgtct tcctgctctg agctttctgg cagcgagtca
    26761 aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac aggatttttc acaggcgtgt
    26821 tcaatctcga taccgttacg cagagctgcg tcgagaatgg tttcaccgct attagcttcc
    26881 agaacagcgc catcaggaca gagatcctga tgaggcaaaa taacaatctt tggcatatta
    26941 aacctcgtcc acggaatggc ctttcagcgc acgacgaacc gactggtcca tgcggcgagc
    27001 ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct tgttcgatgg cgtcaacgtc
    27061 atcgccctgc gccacttcac tcaggtgagc ggcagcatcg tcaatgacct gacgttctgc
    27121 ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc agactttcca gcacacgcgc
    27181 cgcttctact ttttgttctg ccagcattcg ggcttttacg tcctgctcgg catagctcat
    27241 tgagtctttg atcatcgaag cgatttcgct atcggtcaga ccgtaagacg gtttgacctg
    27301 aatagacgcc tcaacgccgg tggatttctc catcgccgtc acgctcaaaa gaccgtcggc
    27361 atcgacctgg aacgtcacgc gaatatgcgc accaccagcc ggtagcgccg gaataccacg
    27421 cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt tcgcgctcac cctgcattac
    27481 atggatagac atcgccgtct gaccatcttt aaaggtggtg aaatcctgag cgcgggccac
    27541 cggaatagtg gtattacgcg gaatcacttt ctccaccagg ccgcccatcg tttcgaggcc
    27601 cagcgacagt gggatcacat caagcagcag catttcgctg tctggcttgt tacccaccag
    27661 aatatccgcc tgaatcgccg cgccaatagc gacgacttta tccgggtcga tggaagtcag
    27721 cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc agcggcacgc gagtagaacc
    27781 gcccaccatc accacttcca gcacttcgtc agcttctaca cccgcgtctt taagcgcgcg
    27841 acgacaagcc agtaaggttc gttttaccag tggcgcgatc agttcattga attgttcacg
    27901 gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg gagtccgcat cgctcagcgc
    27961 gattttggct gcaatggcgg catccagcag ttcacgctga acgcggttat cgctacgatc
    28021 aggaatgccc gcctgctcgc gaatgtaatc cgccagcaga tggtcgaaat catcgccgcc
    28081 gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg ccgcgactta agcgcagaat
    28141 ggaaatatca aacgtcccgc caccgaggtc ataaacagcg atcacgcctt cctgaccgga
    28201 atccagcccg taggcgatag ccgcagcggt cggttcgtta agtaagcgca gaacatgcaa
    28261 ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg gcatcgtcaa agtacgccgg
    28321 aacggtgata actacaccat ccagctcgcc tgccagggct tcagttgccc gcgccgccag
    28381 tgctttgagg atgtccgcag aaacgcgcac cgggttcagc agccccgccg ccgtttcaat
    28441 catcggcagg ccgttttcgc tggcctggaa ttgataaggc agatgcggat agcgttgctg
    28501 gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa ctaatggtgt tggcggtgtc
    28561 aagcgctgcg ttagtacgcg cgtcataacc caccgaatgc ccttgctgtt gatagtgaac
    28621 aacagatggc agcaggtgac ggccttcatg gtcggctaac gtttcggcct gaccgctgcg
    28681 cactgtcgcc accagcgagt tggttgtgcc caggtcaata ccagccgcca gacgacgctg
    28741 atgcggcgca gcactcaaac caggttcact aatttgtaat aaggccatgt ttagcttcca
    28801 gaaattaaaa atcgagcagt ttttcttcga gttgttcggc actgctccgc agtttatcga
    28861 gaaaacgcag cttacgcacg gtatccgccg ccgcgtccca cgcctcgttg tctaactgtt
    28921 caaccatcaa ctgatggcgg gtatcaaaca tctttttcac acgtttgata aagctttcca
    28981 gccgcgcttc atctttcgcc tgttcgatct cgtccagctc ttcgcgcagc tccaactgtt
    29041 ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct ggcgagatca aaaccgtgta
    29101 aagaaagcaa atattccgcg cgcattaacg gatgacgcag cgtttgccag gcctggttaa
    29161 tggttgcaga ttgctgtacg gcggcgagtt gttccgcctg gcttccgctg gcgaatttat
    29221 caggatgata ctgacgttgt agatcctgaa aacgcaggct cagcgcctgg gtatcgagtt
    29281 gataacgggc aggcaagcca aagaaggtga agtaatccat aacattctca gggctgcgat
    29341 ccgccgcgat aacggcgaat caggtaaaac aaaccccacg cgcaggcgac cacggtgggg
    29401 ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa ccacactcat ctttgacgtt
    29461 cgggttggtg aatttaaacc cttcgttcag gccttctttt acgaagtcca gctgcgtacc
    29521 gtccagaaat tgcaggcttt tgccatcgac cacgactttc acgcctttgt cttcaaacac
    29581 gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca taagccatac ctgaacatcc
    29641 ggaggttctc acgcccagac gcaggccaaa ccctttgccg cggttagcca gaaaggtatt
    29701 tactcgcgct gctgcactgt cgctcagtgt aatcgacata accaaacctc aactcttatt
    29761 ttgcttcacg tttgctttta tagtccgcaa tggcggcttt gatcgcgtct tctgccagaa
    29821 tagaacagtg aattttcacc ggcggcagtt caagttcttc agcaatatcg gtatttttga
    29881 tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc ggtgaccagg gagctggaag
    29941 cgatagcaga accgcagccg taagttttaa aacgcgcgtc ttcaatgata ccttcatcgt
    30001 tgactttaat ctgcaacttc atcacgtcgc cacaggccgg tgcccccacc atgccgctgc
    30061 cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg cggattctcg taatggtcga
    30121 taactttttc gctgtaagcc attataaatt ctcctgattc cgataccgat taatgatgag
    30181 cccattcgat gctgttcaga tccacgccct gcttgtacat ttcccacagc ggagaaaggt
    30241 cacgcagacg accgatggat ttacgaacta actcgatggt gtagtcgatc tcttcttcag
    30301 tagtaaaacg acctaaagag aaacggatag agctatgtgc cagctcgtcg ttcagcccca
    30361 gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt acaggcggaa cctgaagaaa
    30421 ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc aacgtagttg aagctgacgt
    30481 tgagaatgtt cggcgcaccg tgttccaggt caccgttcag gtaaacttct tcgatatctt
    30541 tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg ttccatctcg gtcgccatct
    30601 cttcttttgc gatgcgatag gcctcgccca ttccgacgat ctggtgaaca ggcagagtgc
    30661 cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg cgcttcgatg cgtacgcgcg
    30721 gtttacgacg tacatacagc gcaccgatac ctttcgggcc atagattttg tgaccggaga
    30781 aagacatcag gtcaactttc aactggctca ggtcgatagg cagtttgccc acgctctggg
    30841 ttgcatcaac gtgatagata atgccacgag cacggcacat ttcgccgata gccgcgatat
    30901 cctgcaccac gccgatttcg ttatttacgt gcatgatgga aacaaggatg gtgtcatcac
    30961 gcatcgctgc ttcaagttct ttcagatcga taatgccgtt acgctgcggt gccaggtagg
    31021 tgacttcaaa accttcgcgc tccagctgac ggcaggtatc cagtaccgct ttgtgttcgg
    31081 ttttgctggt gatgatgtgc ttgccttttt tctgataaaa gttggctgca cctttgatcg
    31141 ccaggttgtc agattcggtt gcaccagagg taaagacgat ttcacgcgga tcagcgccga
    31201 ccagatcggc aatctgatta cgggcgatat ctaccgcttc ttcagcctgc cagccgaaac
    31261 ggtgagaacg ggaggccggg ttaccaaagg ttccgtccat cgtcataaac tgcatcattt
    31321 tctcggcaac acgcgggtcc accggcgtgg ttgcggagta gtcgagataa atcggtaatt
    31381 tcattgctct ataaactccg tacatcactc aatgcaagga accaggctac cggctggatg
    31441 tacgaccgtg tttacggagc gcgatacgcc ccggcctgat tctaatatct tgttatcttt
    31501 aacctaactg tttcgggttt cagcaagttg acaaacttac gaatctccgg gagcttacat
    31561 aagtaagtga ccggagtgag tatgtgcagt caacgcagat gcaacacgaa acaggacggt
    31621 taaattaagc gcgtaactta acgtcgatcg cgtcttgtat gcgggtgcgt ggcgcgtcgt
    31681 gagtatgctg acgaccagac acatccagca cttcctggtt attaaccagt tcgcctaaag
    31741 taatgttgtt gagaaaaccg gtgagacggt cgctcaaatc acgccacagc gcgtgggtca
    31801 ggcatttatc gccgccctgg caaccgcctt taccctgaca acgggtagca tctacagatt
    31861 cgtcaacggc gctaatcact tcgccaacgg cgatgctgct ggcatctttg cctaacagat
    31921 aaccaccgcc tggtccacgt acgctggaaa ccagaccatt tttacgcaga cgggaaaaca
    31981 gttgttccag ataagaaagg gaaattccct gacgttcgga aatatcagcc aacggtaccg
    32041 ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt cacggcatag cgccctttag
    32101 atgtcagtct catgtcttac ttcacctcaa actcgcccct gcccggggtt ttttattgta
    32161 aaagtggggg tattgcatag cagggtcaag tctgacattc ccgagtaaat tggtcaacta
    32221 tttacttgac tgatttagtc gggtatttaa ccttcagtgc cattttttta tcgtggcgtt
    32281 tgtacctgtt gtccttccag accatgggcg ggttgagggc aattattcgc attagcgctg
    32341 gcctcttccc ctcaccctaa ccctctcccc gaaggggcga ggggaccgtc cgtgcattta
    32401 tctggcttag ccctcaggcc ttattacctt tattctgctg ctctatagaa gccagaatcc
    32461 cgcgcaggat attcaattcc tggctttccg ggcgcgcacg ggtaaacaga cggcgcaatt
    32521 tattcatcac ctgccccgga tggttttcac ggataaaacc ggttgccagc agcgtttgtt
    32581 ccagatgacc ataaaaacgc tccagatcat cgaccagcgg atacggcgtc tcttcatgct
    32641 cgacctgctc gccattttcc tgagttgcca gccaggccat acgcacttca taggcaataa
    32701 cctgaaccgc cattgccaag ttcagcgaac tgtattccgg gtttgccgca atcgcgacat
    32761 gataatggca tttctgcaac tcttcattgg tcaagccgac gcgctcacga ccaaacacca
    32821 gcgccaccgg ggtatttgcc gcttcagcga cgcttttcag accgcattcg cgcgggtcga
    32881 gcatcggcca cggcagcgtg cgggagcgtg cgctggtgcc caccaccagg ctacaaccgg
    32941 ctaacgcttc gtcgagcgta tcgacgatgt gagcattacc aatcacatcg ctggcccctg
    33001 ctgccagggc aatcgcctgg gagtcgggtt tcaccagtgg attaaccagc cacagattgg
    33061 ttaatcccat tgttttcatg gcacgggcaa cagaacccat attgccggtg tgtgacgtct
    33121 ccaccagcac aattcgaata ttttgcagca ttgtctttct tcatctaaag attattcacg
    33181 catcttatca taaaacgaag acagatgccg atctcgctgc tatactctgc gccgttttcc
    33241 cgttctttaa catccagtga gagagaccga tgcatccgat gctgaacatc gccgtgcgcg
    33301 cagcgcgcaa ggcgggtaat ttaattgcca aaaactatga aaccccggac gctgtagaag
    33361 cgagccagaa aggcagtaac gatttcgtga ccaacgtaga taaagctgcc gaagcggtga
    33421 ttatcgacac gattcgtaaa tcttactcac agcacaccat catcaccgaa gaaagcggtg
    33481 aacttgaagg tactgatcag gatgttcaat gggttatcga tccactggat ggcactacca
    33541 actttatcaa acgtctgccg cacttcgcgg tatctatcgc cgttcgtatc aaaggccgca
    33601 ccgaagttgc tgtggtatac gatcctatgc gtaacgaact gttcaccgcc actcgcggtc
    33661 agggcgcaca gctgaacggc taccgtctgc gcggtagcac cgctcgcgat ctcgacggta
    33721 ccattctggc gaccggcttc ccgttcaaag caaaacagta cgccactacc tacatcaaca
    33781 tcgtcggcaa actgttcaac gaatgtgcag acttccgtcg taccggttct gcggcgctgg
    33841 atctggctta cgtcgctgcg ggtcgtgttg acggtttctt tgaaatcggt ctgcgtccgt
    33901 gggatttcgc ggcaggcgag ctgctggttc gtgaagcagg cggcatcgtc agcgacttca
    33961 ccggtggtca taactacatg ctgaccggta acatcgttgc tggtaacccg cgcgttgtga
    34021 aagccatgct ggcgaacatg cgtgacgagt taagcgacgc tctgaagcgt taatgactca
    34081 ggcgggtgat gtcactcacc cgccctcgcc tttcaggcgc tattctgaaa tacttcctca
    34141 ccgctttact ttctttcgtc actctcccac catctttccc cgattaatgg atgataaaga
    34201 agtcacaagg aaaggttatg gcgctgccag tgaacaaacg cgttctcaaa attctgttta
    34261 ttctctttgt tgttgccttc tgcgtttatt tagtgccgcg cgttgccatc aacttcttct
    34321 attatcctga cgataaaatt tacggtcccg acccctggtc ggcggaatcc gtcgaattta
    34381 cggctaagga cggtactcgt ctgcaaggct ggtttattcc ttcttcgacg ggccctgctg
    34441 acaatgccat cgcaaccatc attcatgctc acggcaatgc cggaaatatg tccgcccact
    34501 ggccgctggt cagttggtta cccgagcgta atttcaacgt ttttatgttt gattatcgcg
    34561 ggtttggtaa atcaaaaggc acgccgtccc aggccggatt gctggacgat acgcaaagtg
    34621 ccatcaatgt ggtgcgccat cgcagtgatg taaacccaca acggctggtg ctgttcgggc
    34681 agagtattgg cggggcgaat attcttgctg ttattggtca ggatgatcgt gaaggcatac
    34741 gtgcggtgat cctcgactcc acatttgcct cttatgcaac catcgccaat cagatgatcc
    34801 ccggcagtgg ctacttactt gatgagagtt acagcggcga aaattatatc gccagcgtca
    34861 gcccgatccc gcttttactc attcacggta aagctgatca cgttatccca tggcagcaca
    34921 gcgaaaagtt gtatagcctg gcaaaagaga caaaacggtt gatcctaatc ccggatggcg
    34981 aacacattga tgctttttcc gatcgtcacg gcgatgttta tcgcgaacag atggtggact
    35041 ttatccttag cgcgttgaat ccgcagaact aacccatgat cgctagcacg ataatcattc
    35101 acaaaaccac cttaagacat gctaatccac tggtcagaac agtttaagat gagaaaaatt
    35161 ctgtgacgct tgccaacatt tctgatgatt agcattccct tcgccatctc cctgagcaaa
    35221 ctttagctat tcttatcaat tatgcttatg ggagatctac agatgatgcc tacgcttgct
    35281 ccaccatctg tcctttcggc tccccagcgc cgctgccaga tcttgctgac gctctttcag
    35341 ccggggttga ccgccaccat ggcaaccttc agcgagctta atggtgtgga tgatgatatt
    35401 gccagtcttg atatcagcga aacaggacgg gagatcctgc gctatcatca actcacactg
    35461 acgactggtt atgacggtag ctaccgggtt gaaggtacag tgcttaacca acgtttgtgt
    35521 ttatttcact ggctacgacg tggtttccgt ctgtgtccgt catttattac cagccaattc
    35581 acccccgccc tgaagagtga actgaagcgg cgcggaattg cgcgtaactt ttacgacgat
    35641 accaatctac aagcgttagt gaatctctgc tcccgacggc tgcaaaaacg ctttgaaacg
    35701 cgcgatattc atttcctgtg tctgtatctg caatattgtt tgctgcaaca ccacgctgga
    35761 attacgcctc agtttaatcc gctccaacgt cgctgggcgg aatcctgcct tgaatttcag
    35821 gtagcgcagg aaattggacg ccactggcag cgtcgggcgc tccagcctgt accgcctgat
    35881 gagccactgt ttatggcatt acttttttcc atgttgcggg ttcccgatcc attgcgtgat
    35941 gcgcatcagc gagacagaca attgcgtcag tctatcaaac gtctggtaaa ccattttcgt
    36001 gagctgggaa atgttcgttt ttatgatgaa caggggttat gcgatcagct ttatacccac
    36061 ctcgcccagg cgttaaatcg cagtttgttt gccatcggta ttgataatac cctgccggaa
    36121 gagttcgcca gactgtaccc acgcctggtg cgcaccaccc gcgcggcgct ggccggattt
    36181 gaaagtgaat acggcgtcca tctttctgat gaggaaagcg gtctggtcgc ggtgattttc
    36241 ggtgcctggc taatgcagga aaacgatcta catgaaaaac agattattct actgaccggg
    36301 aatgatagcg agcgagaagc gcagattgag cagcagttac gcgaactaac gttactgccg
    36361 ctcaacatta agcatatgtc ggtaaaggca tttttgcaga caggcgctcc gcgtggcgcg
    36421 gcactgatta ttgcgcctta taccatgcca ttaccgctct tttcaccacc gctgatctat
    36481 acggacctga cgctgacaac acatcaacag gagcagatcc gcaaaatgct cgaatcagca
    36541 tgagggaaca actttcgggc gcaaaaacat tgccggaagc gccaccagcg ccattaccca
    36601 gaacacgccg tggcccagat attgatacag gaaaccggcg aaaacggtca tgatagcgat
    36661 actgccgccc atcgcgacgg cagagtaaac cgcctgtaaa cggatgactt cgctaccctg
    36721 gcgaacagca atatagcgca tggcggccag gtggcagacc gtgaaggtgc cgcaatgcaa
    36781 aatttgcacc actatcaacc acggcaacgc cgtagttgct cccataatgc cccagcgcac
    36841 tacgccgcaa atcgccgaga tcaacagcat atcgcgagca ctacaacggc ggaaaagttt
    36901 attactcagc gcaaagataa tgacttccgc caccacgccc agcgaccaca aataccccac
    36961 cgccgaggcc gagtagccag ctgcctgcca gtaaatggcg ctaaaaccgt aataggccga
    37021 atgtgccccc tgcaataaac aaacgcaggc cagaaagcgc cagttctggc gaaccagcgc
    37081 caaccacgca gaccaaccgg tgctctcctg ctggcgactt gccccttgtg gctgaatcgt
    37141 cggacggatg agaaagccga gcagcatgga tgccacgccc aacgtcaaca gcgcgaggat
    37201 cacccgataa tcaaacatag tgaccagttt gcccgtcagc gccgagccaa tgacaaacgc
    37261 caccgagccc cacagtcgca ctttgccgta atcaagcggg aactgctttt gccacgtatt
    37321 cgccagtgca tcggtcaacg gtaccagcgg tgagaaaaag aggttaaagc caatcatcac
    37381 cagcatcagc cacgctacgt gcgcccccgc ccagaaggcg acagcaaaga gaagtgtcag
    37441 cagtgccagc acgcgcaagg cggaaatcag gcgggaagga tcgctgacgc ggggcgcgat
    37501 gagcaaactc ccgagaaaac gggcaaccag acctgccccc aataacaggc cgatggtttc
    37561 tggcgttaaa ccaatccctt taagccagac gctccagaaa ggtagaaaaa tgccgtaact
    37621 aaaaaagtat gtgaaatagc cgagcgccaa ccagcgcgtg gattgcaaaa ccatgagtcc
    37681 ctcccgtcag gaggcgttag tctggggtta atcgaatgtt ggtgcaagtt gaaaaataag
    37741 aatattcgag cctgcttccc gatgtctgta acgccagttc acaagcacta aaaaactgcg
    37801 atccgcgacg catgttgcaa aaataatgcg taattatgcc gttacgcttg ccaaacgttc
    37861 ctgcacaatg gcgatgaaat cgcgtaatgc cggcttcatc tcccctttct tccacgccat
    37921 cagtaaagca atggaaggca catttccggc aatgggacga aaaacaacct gcccggtatt
    37981 aaaattattc atataaccgg gtatcaaagt gacgcccagc cccatgccca ccagattcat
    38041 ggtcaccaga atattcgttg ccacctggac gatatttggc tggctgtttt cttgcgcaaa
    38101 ccacgcttta acgatcggcg caagcgaacc ggaatacgcc ggatcggtac tgacgaaatt
    38161 cacgccatcc agttgcgcgg cggtgatctc tttttcatgt gctaaagggt gatcaaccgg
    38221 taacaccacc actaatggtt cgtcaaaaag ctccagataa tcaatctccg ggctataaac
    38281 aggatggcgc attaagccga cgtcgagttc accacggcga attttttcct cctgttgcgt
    38341 ggtgattaaa ctcaccagct caatcaaggt gtctggctgt ctgagacgaa acattggtaa
    38401 tacttttggc aataaattca cttccgccga tggcacaaag ccaatggtta attgtctgtc
    38461 ttcctgaaca attttccgcg cccgtaattt ggcattttcc gcttgttcga ggattgccag
    38521 cgcgtcctgg agaaaacatt ctcccgccgc cgtcaacgcg actttgcgct tatccctcac
    38581 cagtaacgga acaccgacac agttttcaag atcgcggatc tggctgctta acgaaggctg
    38641 tgaggtatgc agtttttccg ccgcacgggt aaagttcagt gcctgcgcca ctgcgacgaa
    38701 atagcgtaaa tgccgtagtt ccatcacctt ccccttgtta tcgaaaaaac gtctcagctg
    38761 gtagaaatta actatttcac atattagcaa ccaaccagca acatccttat gacacaaaaa
    38821 tagaaggtca atacatctta tctttcagga ttaaaaaata tgaccacacc ctcagattta
    38881 aacatttacc aactgattga tacccaaaat ggtcgggtca ctccgcgtat ttataccgac
    38941 ccagatattt accaactgga gcttgaacgt attttcggcc gttgctggtt atttctcgcc
    39001 cacgaaagcc agatcccaaa acctggtgat ttctttaaca cctacatggg ggaagatgcg
    39061 gttgtcgtag tgcgtcagaa agacggcagc attaaggcgt ttctcaacca atgccgccac
    39121 cgggccatgc gtgtgagtta tgcagattgc ggcaacactc gcgcctttac ctgtccgtat
    39181 cacggctggt cttatggcat taacggcgag ttgatcgatg taccgctgga acctcgcgcc
    39241 tacccacaag ggttgtgtaa atcccactgg ggactaaacg aagttccttg tgtggagagt
    39301 tataaagggc tgatttttgg caactgggat accagcgcac cgggcctgcg tgattacctg
    39361 ggtgacattg cctggtatct ggatggcatg ctggatcgtc gcgaaggcgg caccgaaatt
    39421 gtcggcggcg tacaaaagtg ggtgatcaac tgtaactgga aattcccggc agagcagttc
    39481 gccagtgacc agtatcatgc tctgttcagc catgcttctg ccgttcaggt attaggggcg
    39541 aaagatgatg gcagcgataa gcgcctcggt gatggacaaa ccgcccgccc ggtgtgggaa
    39601 accgccaaag atgcgctgca atttggtcag gacggtcacg gtagcggttt cttctttact
    39661 gaaaaaccgg atgctaatgt ctgggtcgat ggcgcagttt caagctatta ccgcgaaacc
    39721 tatgccgaag cagaacaacg tttaggtgaa gttcgcgccc tgcgcctggc gggtcataac
    39781 aatattttcc ccacgctttc atggctcaac ggcactgcca cgctccgcgt ctggcatccg
    39841 cgcggccctg atcaagttga agtgtgggcg ttctgtatta ctgacaaagc cgcctccgat
    39901 gaagttaaag ccgcttttga aaacagcgcc actcgtgctt ttggtcctgc tggttttctc
    39961 gagcaggatg actcggagaa ctggtgtgaa atccagaaat tgcttaaagg ccaccgcgcc
    40021 cgcaacagca aactgtgtct ggaaatgggg cttggtcagg aaaagcgccg cgacgacggc
    40081 attcctggca ttactaacta tatcttttca gaaactgccg ctcgtggaat gtaccaacgc
    40141 tgggccgatc ttctgagtag cgaaagctgg caggaagtgc tcgataaaac cgccgcttac
    40201 cagcaggagg tgatgaaatg agtgcgcaag tttcactaga gttacatcac cgcattagcc
    40261 agtttctctt tcacgaagcc agcttactgg acgactggaa atttcgtgac tggctggagc
    40321 agctcgacaa agagattcgt tacaccatgc gcaccacagt taacgcgcaa acacgcgacc
    40381 gccgcaaagg cgtccagcca ccgacaacct ggatttttaa tgacaccaaa gaccagctgg
    40441 agcggcgaat cgcccgtctg gaaacgggca tggcctgggc agaagagccg ccgtcacgca
    40501 cccgtcactt aatcagcaac tgccagataa gcgaaaccga catcccaaac gtatttgctg
    40561 tgcgggtaaa ttatctgctt tatcgggcac aaaaagagcg cgatgaaaca ttctatgttg
    40621 gaacgcgttt cgacaaagtt cgccgtctgg aagatgacaa ctggcgcttg ctggaacggg
    40681 atatcgtcct ggatcaagcg gtaatcactt cccataacct gagtgtactg ttctgatgaa
    40741 tcgaatttat gcgtgtcccg ttgcggatgt gccggagggt gaggctctcc ggatcgatac
    40801 ctcgcccgtc atcgccctgt tcaacgttgg cggcgagttt tatgccatta acgatcgttg
    40861 cagccatggt aatgcgtcga tgtcagaagg gtatctggaa gatgacgcca cggtggagtg
    40921 cccgctacac gccgccagtt tttgcctgaa aacggggaaa gcgttatgcc tgcccgccac
    40981 cgatccgctc accacttatc cagtacacgt tgaaggtggt gacattttca tcgacttacc
    41041 ggaggcgcag ccatgagcga tctgcataac gagtccattt ttattaccgg cggcggatcg
    41101 ggattagggc tggcgctggt cgagcgattt atcgaagaag gcgcgcaagt tgccacgctg
    41161 gaactgtcgg cggcaaaagt cgccagtctg cgtcagcgat ttggcgaaca tattctggcg
    41221 gtggaaggta acgtgacctg ttatgccgat tatcaacgcg cggtcgatca gatcctgact
    41281 cgttccggca agctggattg ttttatcggc aatgcaggca tctgggatca caatgcctca
    41341 ctggttaata ctcccgcaga gacgctcgaa accggcttcc acgagctgtt taacgtcaac
    41401 gtactcggtt acctgctggg cgcaaaagcc tgcgctccgg cgttaatcgc cagtgaaggc
    41461 agcatgattt tcacactgtc aaatgccgcc aggtatcctg gcggcggtgg cccgctgtac
    41521 accgccagta aacatgccgc aaccggactt attcgccaac tggcttatga actggcaccg
    41581 aaagtgcggg tgaatggcgt cggcccgtgt ggtatggcca gcgacctgcg cggcccacag
    41641 gcgctcgggc aaagtgaaac ctcgataatg cagtctctga cgccggagaa aattgccgcc
    41701 attttaccgc tgcaattttt cccgcaaccg gcggatttta cggggccgta tgtgatgttg
    41761 gcatcgcggc gcaataatcg cgcattaagc ggtgtgatga tcaacgctga tgcgggttta
    41821 gcgattcgcg gcattcgcca cgtagcggct gggctggatc tttaaggaag cacgatgaaa
    41881 gaaaaaacga tcattattgt cggtggcggg caagcggcgg caatggctgc ggcctcgcta
    41941 cgccagcaag ggttcaccgg tgagctgcat ctgttttccg atgagcgaca tcttccttat
    42001 gaacgacctc cactctcgaa atccatgttg ctggaagatt ccccgcagtt acagcaggtg
    42061 ttacccgcta actggtggca ggaaaacaat gttcatctgc attccggtgt aaccatcaaa
    42121 acgctgggtc gcgacacacg agagttagtg ttaaccaacg gcgaaagctg gcactgggat
    42181 cagcttttta tagcaatcgg cgcggcagct cgaccgctgc cgttgcttga tgcactggga
    42241 gaacgctgct ttaccctacg ccatgccggt gatgccgcca gactgcgaga agttctgcag
    42301 cccgaacggt cagtcgtgat tgtcggtgcc ggaactattg gtctggaact ggctgccagc
    42361 gccacgcagc gcagatgtaa ggtgacagtg attgaactgg cggcaaccgt catgggccgt
    42421 aatgcaccac cgcccgtgca acgctatctt ttacagcgcc accagcaggc tggtgtgcgc
    42481 attctgctca ataatgccat tgaacatgtg gtcgatggtg aaaaagtaga actgacgctg
    42541 caaagtgggg aaacgcttca ggctgatgtg gtgatttacg gtattggtat cagcgccaac
    42601 gagcaactgg ctcgcgaggc caaccttgat actgccaatg gcattgtcat tgatgaggct
    42661 tgccgcacct gcgatcccgc gatctttgcc ggtggcgatg tggcaatcac tcgtcttgat
    42721 aatggtgcac tacaccgctg cgaaagctgg gaaaacgcca ataaccaggc gcaaattgcc
    42781 gctgccgcaa tgttagggct accgctaccg ctactgccgc cgccgtggtt ctggagcgat
    42841 cagtacagtg ataacttaca gtttattggc gatatgcgtg gcgatgactg gctttgtcgt
    42901 ggcaacccgg aaactcagaa ggcgatttgg tttaatctgc aaaacggcgt gcttatcggt
    42961 gcggtcacgc tgaatcaggg gcgtgagatt cgcccaattc gcaaatggat ccagagcggc
    43021 aaaacgtttg atgcgaaact gctgatagat gagaacatcg cgcttaaatc actgtaacca
    43081 ggataattag cgaatatctc aacgcctggg gcgtggcgag gtgcaagagt gtgtattacg
    43141 tttaaatcac attatcttgc aaagggattg gttatgaaca cactacgttg gtgatgctgc
    43201 caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta
    43261 tcagctgtcc ctcctgttca gctactgacg ggg
//
LOCUS       DAZRUF010000009        43267 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZRUF010000009 DAZRUF010000000
VERSION     DAZRUF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43267
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.009"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            296..1585
                     /gene="sdaC"
                     /locus_tag="ACHXOL_000393"
     CDS             296..1585
                     /gene="sdaC"
                     /locus_tag="ACHXOL_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR6807470.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1643..3010
                     /gene="sdaB"
                     /locus_tag="ACHXOL_000394"
     CDS             1643..3010
                     /gene="sdaB"
                     /locus_tag="ACHXOL_000394"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR6807471.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3122..3877
                     /gene="xni"
                     /locus_tag="ACHXOL_000395"
     CDS             3122..3877
                     /gene="xni"
                     /locus_tag="ACHXOL_000395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR6807472.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3982..5130)
                     /gene="fucO"
                     /locus_tag="ACHXOL_000396"
     CDS             complement(3982..5130)
                     /gene="fucO"
                     /locus_tag="ACHXOL_000396"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR6807473.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5158..5805)
                     /gene="fucA"
                     /locus_tag="ACHXOL_000397"
     CDS             complement(5158..5805)
                     /gene="fucA"
                     /locus_tag="ACHXOL_000397"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR6807474.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6352..7668
                     /gene="fucP"
                     /locus_tag="ACHXOL_000398"
     CDS             6352..7668
                     /gene="fucP"
                     /locus_tag="ACHXOL_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR6807475.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            7701..9476
                     /gene="fucI"
                     /locus_tag="ACHXOL_000399"
     CDS             7701..9476
                     /gene="fucI"
                     /locus_tag="ACHXOL_000399"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR6807476.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGSVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9585..11003
                     /gene="fucK"
                     /locus_tag="ACHXOL_000400"
     CDS             9585..11003
                     /gene="fucK"
                     /locus_tag="ACHXOL_000400"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR6807477.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            11005..11427
                     /gene="fucU"
                     /locus_tag="ACHXOL_000401"
     CDS             11005..11427
                     /gene="fucU"
                     /locus_tag="ACHXOL_000401"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR6807478.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11485..12216
                     /gene="fucR"
                     /locus_tag="ACHXOL_000402"
     CDS             11485..12216
                     /gene="fucR"
                     /locus_tag="ACHXOL_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR6807479.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12260..13360)
                     /gene="rlmM"
                     /locus_tag="ACHXOL_000403"
     CDS             complement(12260..13360)
                     /gene="rlmM"
                     /locus_tag="ACHXOL_000403"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR6807480.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13353..13748)
                     /locus_tag="ACHXOL_000404"
     CDS             complement(13353..13748)
                     /locus_tag="ACHXOL_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR6807481.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKCKGVSHE"
     gene            complement(13767..14684)
                     /gene="gcvA"
                     /locus_tag="ACHXOL_000405"
     CDS             complement(13767..14684)
                     /gene="gcvA"
                     /locus_tag="ACHXOL_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR6807482.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15035..15262)
                     /locus_tag="ACHXOL_000406"
     CDS             complement(15035..15262)
                     /locus_tag="ACHXOL_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR6807483.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            15454..16659
                     /gene="csdA"
                     /locus_tag="ACHXOL_000407"
     CDS             15454..16659
                     /gene="csdA"
                     /locus_tag="ACHXOL_000407"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR6807484.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16659..17102
                     /gene="csdE"
                     /locus_tag="ACHXOL_000408"
     CDS             16659..17102
                     /gene="csdE"
                     /locus_tag="ACHXOL_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR6807485.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17153..17959)
                     /gene="tcdA"
                     /locus_tag="ACHXOL_000409"
     CDS             complement(17153..17959)
                     /gene="tcdA"
                     /locus_tag="ACHXOL_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR6807486.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18036..19133)
                     /gene="mltA"
                     /locus_tag="ACHXOL_000410"
     CDS             complement(18036..19133)
                     /gene="mltA"
                     /locus_tag="ACHXOL_000410"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR6807487.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19342..19418
                     /locus_tag="ACHXOL_000411"
     tRNA            19342..19418
                     /locus_tag="ACHXOL_000411"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19376..19378,aa:Met,seq:cat)
     gene            complement(19493..20746)
                     /gene="amiC"
                     /locus_tag="ACHXOL_000412"
     CDS             complement(19493..20746)
                     /gene="amiC"
                     /locus_tag="ACHXOL_000412"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR6807488.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            20978..22309
                     /gene="argA"
                     /locus_tag="ACHXOL_000413"
     CDS             20978..22309
                     /gene="argA"
                     /locus_tag="ACHXOL_000413"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR6807489.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22371..24197)
                     /gene="recD"
                     /locus_tag="ACHXOL_000414"
     CDS             complement(22371..24197)
                     /gene="recD"
                     /locus_tag="ACHXOL_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR6807490.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(24197..27739)
                     /gene="recB"
                     /locus_tag="ACHXOL_000415"
     CDS             complement(24197..27739)
                     /gene="recB"
                     /locus_tag="ACHXOL_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR6807491.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(27732..30620)
                     /gene="ptrA"
                     /locus_tag="ACHXOL_000416"
     CDS             complement(27732..30620)
                     /gene="ptrA"
                     /locus_tag="ACHXOL_000416"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR6807492.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKRDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYPQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(30796..34164)
                     /gene="recC"
                     /locus_tag="ACHXOL_000417"
     CDS             complement(30796..34164)
                     /gene="recC"
                     /locus_tag="ACHXOL_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR6807493.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34177..34500)
                     /locus_tag="ACHXOL_000418"
     CDS             complement(34177..34500)
                     /locus_tag="ACHXOL_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR6807494.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(34485..34892)
                     /locus_tag="ACHXOL_000419"
     CDS             complement(34485..34892)
                     /locus_tag="ACHXOL_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR6807495.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(34889..35452)
                     /locus_tag="ACHXOL_000420"
     CDS             complement(34889..35452)
                     /locus_tag="ACHXOL_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR6807496.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35443..35913)
                     /locus_tag="ACHXOL_000421"
                     /pseudo
     CDS             complement(35443..35913)
                     /locus_tag="ACHXOL_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36098..36892)
                     /gene="thyA"
                     /locus_tag="ACHXOL_000422"
     CDS             complement(36098..36892)
                     /gene="thyA"
                     /locus_tag="ACHXOL_000422"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR6807497.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(36899..37774)
                     /gene="lgt"
                     /locus_tag="ACHXOL_000423"
     CDS             complement(36899..37774)
                     /gene="lgt"
                     /locus_tag="ACHXOL_000423"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR6807498.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(37925..40171)
                     /gene="ptsP"
                     /locus_tag="ACHXOL_000424"
     CDS             complement(37925..40171)
                     /gene="ptsP"
                     /locus_tag="ACHXOL_000424"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR6807499.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40184..40714)
                     /gene="rppH"
                     /locus_tag="ACHXOL_000425"
     CDS             complement(40184..40714)
                     /gene="rppH"
                     /locus_tag="ACHXOL_000425"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR6807500.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(41067..41213)
                     /gene="ygdT"
                     /locus_tag="ACHXOL_000426"
     CDS             complement(41067..41213)
                     /gene="ygdT"
                     /locus_tag="ACHXOL_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR6807501.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            41399..42088
                     /gene="mutH"
                     /locus_tag="ACHXOL_000427"
     CDS             41399..42088
                     /gene="mutH"
                     /locus_tag="ACHXOL_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR6807502.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42157..42870
                     /locus_tag="ACHXOL_000428"
     CDS             42157..42870
                     /locus_tag="ACHXOL_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR6807503.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43008..43226
                     /gene="ygdR"
                     /locus_tag="ACHXOL_000429"
     CDS             43008..43226
                     /gene="ygdR"
                     /locus_tag="ACHXOL_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR6807504.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        10437 a        11654 c        10754 g        10422 t
ORIGIN      
        1 cgccccggaa gaagtcaaat gtcgtttaat gggcaaatat tgcccttaaa ttctctttta
       61 cttttgattt acagagtaaa gcgttgggat aatctatctt ccaagtagat tattgtattt
      121 gggatcaaga tcactgatag atacataact tgtgtgtatc tttccgccct caaattatta
      181 cggcggtaaa tgattaagcc atcgccgata gacagatttc atttttacgg tcaggcacct
      241 tcccgagctg aactggctaa aagctgaatt atttgcattc ctccaggaga aatagatgga
      301 aacgactcaa accagcacga ttgcgtcgaa agactctcgt agtgcctggc gcaagacaga
      361 caccatgtgg atgctgggcc tttacggcac ggcaatcggc gcgggcgtgc tgttcctgcc
      421 aatcaacgcc ggtgttggcg gtatgatccc gctgatcatc atggctatcc ttgcgttccc
      481 gatgacattt tttgctcacc gcggcctgac tcgcttcgta ctgtctggta aaaacccagg
      541 cgaagacatc accgaggttg tagaagaaca ctttggtatt ggcgcaggta aactgattac
      601 cctgctctac ttcttcgcta tctacccgat cctgctggtt tatagcgtgg caatcaccaa
      661 tacagttgaa agcttcatgt ctcaccagct gggtatgaca ccaccgccgc gtgcgattct
      721 gtcgctgatc ctgatcgtgg gtatgatgac catcgttcgc ttcggtgagc agatgatcgt
      781 taaagcaatg agtattctgg tattcccgtt tgttggcgta ctgatgctgc tggctctgta
      841 cctgatcccg cagtggaacg gcgcagcact ggaaacgctg tctctggaca ctgcatctgc
      901 aaccggaaac ggtctgtgga tgaccctgtg gctggcaatt ccggttatgg tgttctcttt
      961 caaccactct ccgatcatct cttctttcgc cgttgcgaag cgtgaagagt acggcgatat
     1021 ggcagaacag aaatgctcga agatcctggc attcgcacac atcatgatgg tgctgaccgt
     1081 aatgttcttc gtcttcagct gtgtactgag cctgactccg gcagacctgg ctgcggctaa
     1141 agagcagaac atctcgattc tgtcttacct ggctaaccac tttaacgcac cggttatcgc
     1201 gtggatggct ccgattatcg cgattatcgc tatcaccaaa tccttcctcg gccactacct
     1261 gggcgcacgt gaaggcttca acggtatggt gattaaatct ctgcgtggta aaggtaagtc
     1321 tatcgaaatc aacaagctga accgtatcac tgcgctgttc atgctggtaa cgacctggat
     1381 tgttgccacc ctgaacccga gcatcctggg tatgattgaa accctgggcg gcccaatcat
     1441 cgcgatgatc ctgttcctga tgccgatgta cgcaattcag aaagtaccgg caatgcgtaa
     1501 gtacagcggt cacatcagca acgtattcgt tgtcgtgatg ggtctgattg caatctccgc
     1561 aatcttctac tctctgttca gctaagtcct ttcgcgccgc tttcgggcgg cgcttcctcc
     1621 gttttaacgc gatgtatttc ctatgattag cgtattcgat attttcaaaa tcggcattgg
     1681 cccttccagt tctcataccg ttggaccaat gaaagcgggt aaacaattta ccgacgatct
     1741 gattgcccgt aatctgctta aagacgtgac ccgcgtggtg gttgacgtgt acggctcgct
     1801 ctctctgacc ggtaaaggcc accacactga tatcgccatt attatgggcc tggcgggtaa
     1861 cctgccggat accgtggata tcgattccat ccctggtttt attcaggatg tgaatactca
     1921 tggtcgcctg atgctggcaa acggtcagca tgaagtggag ttcccggttg atcagtgcat
     1981 gaacttccat gcagacaacc tttctctgca tgaaaacggt atgcgcatta ccgcgctggc
     2041 gggcgataaa gtcgtttaca gccagactta ctactctatt ggcggtggct ttatcgttga
     2101 tgaagagcat tttggccagc agaatagcgc accggttgaa gttccttatc cgtacagttc
     2161 agcagccgat ctgcaaaaac attgtcagga aaccgggctg tcactctctg gcctgatgat
     2221 gaaaaatgag ctggcgctgc acagcaaaga agagctggaa cagcacctgg cgaacgtatg
     2281 ggaagtcatg cgtggcggta ttgagcgcgg tatttccacc gaaggcgtgt tgcctggcaa
     2341 actgcgcgtt ccacgccgtg ctgcggcact acgccggatg ctggtcagcc aggataaaac
     2401 caccactgac ccgatggcgg ttgttgactg gatcaacatg tttgcactgg cagtgaacga
     2461 agagaacgct gctggcggac gcgtggtgac tgcgccgact aacggtgcgt gcgggattat
     2521 cccggcagtg ctggcgtact acgacaagtt catccgcgaa gtgaacgcta actcactggc
     2581 tcgttacctg ctggtagcca gtgccattgg ttctctttat aagatgaacg cgtcaatttc
     2641 tggtgctgaa gtcggctgcc agggtgaagt tggtgtggcg tgctcaatgg cggcggctgg
     2701 tctggcagaa ctgttaggtg caagcccggc gcaggtgtgc atcgcggcgg aaatcgccat
     2761 ggagcacaac ctcggtctga cgtgtgaccc ggtcgccgga caggtccagg tgccatgcat
     2821 cgagcgtaac gccattgcgg cagtaaaagc ggtgaacgcc gcacgtatgg cgctacgccg
     2881 taccagcgag ccgcgcgtct gcctcgataa agttatcgaa accatgtacg aaacaggtaa
     2941 agatatgaac gccaagtacc gcgaaacctc tcgcggcggc ctggcaatga agatcgttgc
     3001 ctgcgattaa tcgctctcca gaggcctcgt tttgcgaggc ctcttcccga tttctcatcc
     3061 agccgtagcc tgttccggca tcgaatgtta cccttatcgc ctgatcttta agggggttat
     3121 cgtggctgtt catttgctta ttgtcgatgc actgaatctt attcgtcgca ttcatgccgt
     3181 tcagggatcg ccctgtgtcg aaacctgcca gcatgcgctc gatcagctca ttatgcacag
     3241 ccagccaacc cacgcggtcg ccgtttttga tgatgaaaac cgcagtagcg gctggcgtca
     3301 tcagcgttta ccagattaca aagcgggtcg accgccaatg ccggaagagt tgcacgacga
     3361 gatgcctgca ttacgcgccg cctttgagca acgcggcgtc ccgtgctggt caaccagcgg
     3421 caacgaagcc gatgacttag ccgccacgct ggcggtcaaa gtgacacagg ccgggcatca
     3481 ggcaacgatt gtttcgacag ataaaggcta ctgtcagtta ctttcaccga cattacgtat
     3541 tcgtgattac ttccagaaac gttggctgga tgcgccattt atcgataaag aatttggcgt
     3601 tcaaccgcag cagttgcccg attactgggg acttgcgggg atcagcagtt caaaggtacc
     3661 gggtgttgcg ggaatcggac caaaaagcgc cacgcagctg ctggtcgagt ttcagagtct
     3721 ggaagggata tatgagaatc tggatgcggt tgccgaaaag tggcgcaaaa aattagaaac
     3781 ccataaagag atggcgtttc tgtgccgcga tattgcccgc ttacaaaccg atttgcatat
     3841 cgacggcaat ttacagcaat tgcggttggt acggtaacgg cgagccggat gcaaacgatt
     3901 atcaacacat tgaatttaat gcacttatat accggataag acgcggctag cgtcgtatcc
     3961 ggcattatca catcagcgca tttaccaggc ggtatggtaa agctctacaa tatcctcaag
     4021 cgttgcttca cgcgggttgc caccggtaca aacatcatcc agtgccgcct gcgccagtgc
     4081 cggaatgtct tccttgcgta caccaacatc acgcaaatgt ggcggaatac cgacatcacg
     4141 gttgagagca aacaccgctt caacagcggc attacgcgcc tcttccaggc tcataccttc
     4201 cactttcacg cccataacgc gcgcgatatc gcggtacttc tcaccggtaa agtcagcgtt
     4261 atagcgcatg acatgcggta acaggatggc gttcgcaaca ccgtgtggag tgttgtaaaa
     4321 cgcgcccagt ggatgcgcca taccatgcac caaccctaac ccaacattcg agaagcccat
     4381 acccgcaaca tactgcccga gcgccatttc ttctccggca tccttatcac cagcaaccga
     4441 tcctcgcagc gccccagcaa tgatttcaat cgctttaatg tgcagtgcat cggttagcgc
     4501 ccacgcgcca cgggtaatat acccctcaat agcatgagtg agcgcatcga cacccgtcgc
     4561 agctttcagc gctggaggca taccatccat catgtcagcg tcaataaacg ccacctgcgg
     4621 gatatcatgc ggatcaacgc aaacaaactt gcgccgtttc tcttcgtcag tgatcacgta
     4681 gttaatggtc acttctgccg cagtacctgc tgtggtagga attgccagaa tcggtacact
     4741 gggtttattg gtcggggaaa gcccttccag gctacgcaca tcggcaaact ccgggttgtt
     4801 gctgataatg ccaatcgctt tacaagtatc ctgtggagaa ccaccaccaa tagcgatcag
     4861 gtaatccgcg ccgctattct ggaatacacc gagcccttct ttgacgacag taattgttgg
     4921 gttgggcact acgccgtcgt aaatcgccca tgccagccct gcagcatcca tcttatcggt
     4981 cactttcgcc accacgccgc attgcaccag cgttttatcg gtgacgatca gcgccttctg
     5041 ataaccacgg cgtttcacct catcggttaa agccccaaca gcaccccgac caaaccatgc
     5101 cgtttcgttc agaatcattc tgttagccat catccttctc cttgttgctt tacgaaatta
     5161 ctcttcaatc cgtaacccat aggttttgaa tttctccagc actacggcaa tctcttcatc
     5221 gctcagcact ggcaccgggt ccgtaatcgc cagggtcgtc acgtaaagtt gcgccagcac
     5281 ttcaacttca tgcgccagcc ataacgcttt ttccagattc acctcacaag cgataagccc
     5341 atgatgttgt aacaaagttg ccttacgatt tttgagagcc agcgcaacat gttcagaaag
     5401 ttcgcgtgtt ccaaaggtcg cataaggcgc gcaaggaata gaattaccac ctgccgccgc
     5461 aatcatgtag tgaatagcgg ggatcggtcg gttaagaatg gaaactgctg tgcaatgaac
     5521 ggcatgattg tgaacaaccg cgttggcatc cggtctgctt tgataggccg ccatatggaa
     5581 acgccattcg cttgagggga gctttccttc ctcatgttta ccgttgccat caataaagac
     5641 aatatgcgac tccgtcagtt tttcatatgg aatgcctgta ggcgtaatca gcatcccatc
     5701 ctgataacgt acactgacgt tccccgctgt cccctggttc agtcccaggc gggtcatttc
     5761 caggcaagtg tcaataatct gacgagcaag tttatttcgt tccattagct acctctctct
     5821 gattcaaaac agggcaataa tgttgttcct ttcacactat tgaattagcc gtttaattac
     5881 ccaccatctt cttcctgatt aacaagaaag aaattcacaa gcttatattt tgtgacctag
     5941 ctcaactaat cacagtaaat aactgcaagt tctcttttta taaccccatt aaaaatgacc
     6001 gctcttaaaa atatttatca aaacggtcat ttttctattc acccaagcca ggaatgcccg
     6061 ttttcggcat aaacaattaa tacagtcatc tggtttgtgt tttttatgat ttattttctg
     6121 aaacgggcat gaaatttcga ttattaaagt gatggtagtc acataaagtc accttccagc
     6181 taataagtgt gaccgccgtc atattacaga gcgtttttta tttgaaaatg aatccatgag
     6241 ttcatttcag acaggcaaat attcactgat atgaagcccg aactcgctgg ttttgcactt
     6301 ttgaaaacat aaccgattac gtgcttaagc ttctgaacct aagaggatgc tatgggaaac
     6361 acatcaatac aaacgcagag ttaccgtgcg gtagataaag atgcagggca aagcagaagt
     6421 tacattattc cattcgcgct gctgtgctca ctgttttttc tttgggcggt agccaataac
     6481 cttaacgaca ttttattacc tcaattccag caggctttta cgctgacaaa tttccaggct
     6541 ggcctgatcc aatcggcctt ttactttggt tatttcatta tcccaattcc tgctgggata
     6601 ttgatgaaaa aactcagtta taaagcagga attattactg ggttattttt atatgccctg
     6661 ggcgctgcat tattctggcc cgctgcagaa ataatgaact acaccttatt tttagttggc
     6721 ctatttatta ttgcagccgg attaggttgt ctggaaactg ccgcaaaccc ttttgttacg
     6781 gtattagggc cggaaagcag tggtcacttc cgcttaaatc ttgcgcaaac atttaactcg
     6841 tttggcgcaa ttatcgcggt tgtctttggg caaagtctta ttttgtctaa cgtgccacat
     6901 caatcgcaag acgttctcga taaaatgtct ccagagcaat tgagtgcgta taaacacagc
     6961 ctggtattat cggtacagac accttatatg atcatcgtgg ctatcgtgtt actggtcgcc
     7021 ctgctgatca tgctgacgaa attcccggca ttgcagagtg ataatcacag tgacgccaaa
     7081 caaggatcgt tctccgcatc gctttctcgc ctagcgcgta ttcgccactg gcgctgggca
     7141 gtattagcgc aattctgcta cgttggcgca caaacggcct gctggagcta tttgattcgc
     7201 tacgctgtag aagaaattct aggtatgact gcaggctttg ccgctaacta tttaaccgga
     7261 accatggtgt gcttctttat tggtcgtttc accggtacct ggctcatcag tcgcttcgca
     7321 ccacacaaag tcctggccgc ctacgcatta atcgctatgg cactgtgcct gatctcagcc
     7381 ttcgctggcg gtcatgtggg cttaatagcc ctgactttat gcagcgcctt tatgtcgatt
     7441 cagtacccaa caatcttctc gctgggcatt aagaatctcg gccaggacac caaatacggt
     7501 tcgtccttca tcgttatgac cattattggc ggcggtattg tcactccggt catgggtttt
     7561 gtcagtgacg cggcgggcaa catccccact gctgaactga tccccacact ctgcttcgcg
     7621 gtcatcttta tctttgcccg tttccgctct caaacggcaa ctaactgaac atattttccg
     7681 aataaagtga ggaatctgta atgaaaaaaa tcagcttacc gaaaattggt atccgcccgg
     7741 ttattgacgg tcgtcgcatg ggtgttcgtg agtcgcttga agaacaaaca atgaatatgg
     7801 cgaaagctac ggccgcactg ctgaccgaga aactgcgcca tgcctgcgga gctgccgtcg
     7861 agtgtgtcat ttccgatacc tgtatcgcgg gtatggctga agccgctgct tgcgaagaaa
     7921 aattcagcag ccagaatgta ggcctcacca ttacggtaac gccttgctgg tgctatggca
     7981 gtgaaaccat cgacatggat ccaacccgcc cgaaagccat ttggggcttt aacggcactg
     8041 aacgtcccgg cgctgtttac ctggcagcgg ctctggcagc tcacagccag aaaggcatcc
     8101 cggcattctc catttacggt catgacgttc aggatgccga tgacacatcg attcctgccg
     8161 atgttgaaga aaaactgctg cgctttgccc gcgccggttt ggccgtcgcc agcatgaaag
     8221 gtaaaagcta tctgtcgctg ggcggcgttt cgatgggtat cgccggttct attgttgatc
     8281 acaacttctt tgaatcctgg ctgggaatga aagtccaggc ggtggatatg accgaactgc
     8341 gtcgccgtat cgatcagaag atttacgacg aagccgaact ggaaatggca ctgacctggg
     8401 ctgataaaaa cttccgctat ggcgaagatg aaaataacaa acagtatcaa cgcaatgccg
     8461 agcaaagccg cgcagttctg cgcgaaagtt tactgatggc gatgtgtatc cgcgacatga
     8521 tgcaaggcaa cagcaaactg gccgatattg gtcgcgtgga agaatcactt ggctacaacg
     8581 ccatcgctgc gggcttccag gggcaacgtc actggaccga tcaatatccc aatggtgaca
     8641 ccgccgaagc gatcctcaac agttcatttg actggaatgg cgtgcgcgaa ccctttgtcg
     8701 tggcgaccga aaacgacagt cttaacggcg tggcaatgct aatgggtcac cagctcaccg
     8761 gcaccgctca ggtatttgcc gatgtgcgta cctactggtc accagaagca attgagcgtg
     8821 taacggggca taaactcgat ggactggcag aacacggcat catccatttg atcaactcag
     8881 gttctgctgc gctggacggt tcctgtaaac aacgcgacag cgaaggtaac ccgacgatga
     8941 agccacactg ggaaatctct cagcaagagg ctgacgcttg cctcgccgct accgaatggt
     9001 gcccggcgat ccacgaatac ttccgtggcg gcggttactc ttcccgcttc cttaccgaag
     9061 gcggcgtccc gttcaccatg actcgtgtca acatcatcaa aggcctggga tcggtactgc
     9121 aaatcgcgga aggctggagc gtggaattgc cgaaggatgt gcatgacatc ctcaacaaac
     9181 gcaccaactc aacctggcca accacctggt ttgcaccgcg cctcaccggt aaagggccgt
     9241 ttacggatgt gtactcggta atggcgaact ggggcgctaa ccatggggtt ctgaccatcg
     9301 gccacgttgg cgcagacttt atcactctcg cctccatgct gcgtatcccg gtatgtatgc
     9361 acaacgttga agagaccaaa gtgtatcgtc cttctgcctg ggctgcgcac ggcatggata
     9421 ttgaaggcca ggattaccgc gcttgccaga actacggtcc gttgtacaag cgttaataca
     9481 ttactccctg atgtgatgcc cggtcgctcc ggctaccggg cctgaacaag caagagtggt
     9541 tagccggata agcaatgtta tccggctata ttgcaggagc gattatgaaa caagaagtta
     9601 tcctggtact cgactgtggc gcgaccaatg tcagggccat cgcggttaat cggcagggaa
     9661 aaattgttgc ccgcgcctca acgcctaatg ccagcgatat cgcgatggaa aacaacacct
     9721 ggcaccagtg gtctttagac gccattttgc aacgctttgc tgattgctgt cggcaaatca
     9781 atagtgaact gactgaatgc cacatccgcg gtatcgccgt caccaccttt ggtgtggatg
     9841 gcgctctggt agataagcaa ggcaatctgc tctatccgat cattagctgg aaatgtccgc
     9901 gaacagcagc ggttatggac aatattgaac agttgatctc cgcacagcgg ttgcaggcta
     9961 tttctggcgt cggagccttt agtttcaata cgttatataa gttggtgtgg ttgaaagaaa
    10021 atcatccaca actgctggaa cgcgcgcacg cctggctctt tatttcgtcg ctgattaacc
    10081 accgtttaac cggcgaattc actactgata tcacgatggc cggaaccagc cagatgctgg
    10141 atatccagca acgcgatttc agtccgcaaa ttttacaagc caccggtatt ccacgccgac
    10201 tcttccctcg tctggtggaa gcgggtgaac agattggtac gctacagaac agcgccgcag
    10261 caatgctcgg cttacccgtt ggcataccgg tgatttccgc aggtcacgat acccagttcg
    10321 ccctttttgg cgctggtgcc gaccaaaatg aacccgtgct ctcttccggt acatgggaaa
    10381 ttttaatggt ccgtagcgcc caggttgata cttcgctgtt aagtcagtac gccgattcta
    10441 cctgcgaact ggatagccag gcagggttgt ataacccagg tatgcaatgg ctggcatccg
    10501 gcgtgctgga atgggtgaga aaactgttct ggacggctga aacaccctgg caaatgttga
    10561 ttgaagaagc tcgtctgatc gcacctggcg cggatggtgt gaaaatgcag tgtgatttat
    10621 tgtcgtgtca gaacgctggc tggcaaggag tgacgcttaa taccacgcgg gggcatttct
    10681 atcgcgcggc gctggaaggg ttaactgcgc aattacagcg caatctacag atgctggaaa
    10741 aaatcgggca ctttaaagcc tctgaattat tgttagtcgg tggaggaagt cgcaacacat
    10801 tgtggaatca gattaaagcc aatatgcttg atattccggt aaaagttctc gacgatgccg
    10861 aaacgaccgt cgcaggagct gcgctgttcg gttggtatgg cgtaggggaa tttaacagcc
    10921 cggaagaagc ccgtgcacag attcattatc agtaccgtta tttctacccg caaactgaac
    10981 ctgaatttat agaggaagtg tgaaatgctg aaaacaattt cgccgttaat ttctcccgaa
    11041 ctattgaaag tgctggcaga gatgggacat ggagatgaaa ttattttttc cgatgctcac
    11101 tttcccgccc attcgatggg accgcaggtg atccgcgctg atggcctgtt ggtgagcgac
    11161 ttgctccaag cgattatccc attatttgag ctggacagtt atgccccgcc gctggtgatg
    11221 atggcagcgg tagaaggtga cactctcgat cctgaagtag aacgacgtta ccgtaatgcg
    11281 ctttcactac aagccccgtg tcctgacatc atccgcatca atcgttttgc gttttatgaa
    11341 cgggcgcaaa aagcctttgc gatcgttatc acaggcgaac gagcgaagta cgggaatatt
    11401 cttttaaaaa aaggggtaac accgtaatct catatcggta cgcccgcatg acgcgggcgg
    11461 ttatcgaatg atggggtgaa aaatatgaaa gcggcacgcc agcaagcgat agtcgacctg
    11521 ctgctgaacc ataccagcct gaccacggaa gctctctctg aacagctaaa ggtcagtaaa
    11581 gaaaccattc gtcgcgatct caatgaatta cagacgcagg gtaaaattct gcgcaatcat
    11641 ggacgcgcta aatatatcca ccgtcaaaat caagacagtg gcgatccctt tcacatcagg
    11701 ctgaaaagcc attatgcgca taaagcagat atcgcgcgcg aggcgctcgc gtggattgaa
    11761 gaagggatgg tgatagcctt agacgccagt tcaacttgct ggtatctggc acgccagttg
    11821 cctgacatca acattcaggt cttcaccaat agccatccga tttgccatga actcggtaaa
    11881 cgcgaacgca ttcaactgat cagttccggc ggcacacttg agcgcaaata tggctgttac
    11941 gtcaatccct cgctgatttc ccaacttaaa tcgctggaaa tcgatctgtt tattttttct
    12001 tgtgaaggga tcgatagcag cggcgcactg tgggactcca atgcgatcaa cgctgattac
    12061 aaatcgatgc tattaaaacg tgccgcgcaa tcgttgttat tgattgataa aagtaaattt
    12121 aatcgttcag gggaagcccg catcgggcat ctggatgagg taacgcacat tatttctgat
    12181 gagcgccagg ttgcaacttc tttggtaaca gcctgacgga aagggtagca ggccgaagac
    12241 gacgccccgg ccttgcctgt tatcgctcgt cgcgacgacc acccaccgca gcccagatgc
    12301 ggcggacgtg caccgtcact tcttcgcgat cgtgatacaa ctgccgcgcc tgaatctgag
    12361 catttatgcc atgttcatca agctgtgcct gaatatacgc cagattgtgt gatacttctt
    12421 cgtagcgttt tttcatcggc agtttgagat tgaaaatggt ttcacggcac cagccattaa
    12481 ccagccactg cgccatcaat gccgcaactt tagccggttt ttcaaccata tcgcatacca
    12541 tccaggagat attgctgcgt gtcggacgga atttgaaacc gtcttcccgc agccacgtca
    12601 cctgcccggt atccatcaga ctttgcgcca tcgggccgtt gtcgacggaa taaacccaca
    12661 tgttgcgctt caccagttgg taggtccagc cgccagggca agcgcctaaa tccaccgccc
    12721 acatcccgtt cgccaggcgt tcatcccact catccgcagg aataaacaca tgaaatgcct
    12781 cttccagttt gagcgtggaa cgactcggcg cctctgccgg aaatttcagg cgcggaatgc
    12841 ccatatagaa cggcgaatta ttgttgctgt atgagtaacc ggtatagcag cagcctggtg
    12901 caataaagaa tacatgcaca accggacgct tcggcgtttc atagttcgcc agcaccccgg
    12961 catcgcgcaa ggcagcgcgt agcggcacgg tgaatttacg gcagaatttc agcaactctt
    13021 tgctttcgtt ggtatcggca acttcaacac gcagttcacc gcccttctct actacgccct
    13081 gcaacatgtc gacaatgggg gtaatacgat cttctggcgg caaatgctgc aagagttccc
    13141 ccaccacaaa ccactggcgg gcaaaaatta atgaactgaa cggcagctca cggattaact
    13201 tatcgccatc atcaggttga taacattcat aaatgacata acccgcattc tctttcacgc
    13261 gggcaaaacc gaaaatttcc cgctggccgg ctttatcggt aatttctgcg gcgcactctt
    13321 tttcaaagcc cggacggcac agcaatacaa ccttattcat gacttacgcc cttgcacttt
    13381 aaacggatag caccaactaa cattaacgcc cagcccgcga ggaagctcac gccgccaacc
    13441 ggtgtgacaa atgcccacaa acgcagatgg gacagcgcca ggcaataaag gctgccgctg
    13501 aacaacaccg tgcctaacgc gaggaaaacg ctactccagt aaaaccagat actgatgcga
    13561 cgctgcattg ccaccgccag acctaagatc gccagcgtat gaaacgcctg gtattcgagg
    13621 ccggtctgga tccagcccat ctcaacggcc cccatggttt tacttaacac atgcgcgcca
    13681 aaagcgccca gagccacaaa aatgaagccg ctaatggcgg cgaaaatcag cataaaacgg
    13741 ctggtcatgg tcgtacccta cgtaaattat tgttcataac gaaagcggaa tttttcttgt
    13801 tcagcagcgg ctttcgccag gatccattgg cgaaaggcgg ctattttacc cagttctgcc
    13861 tggctgtcat gacaaaccag ataaaaagcg tttttactga ccagaacatc attaaacggg
    13921 caaacaagac gtccggcctc gatttcagat tgcgccatca cgttatttgc cagcgccact
    13981 ccctgcccgt gaatagccgc ttgcagcacc atggcgctat gactaaaaat tggcccttgc
    14041 tgaacgttga tatgatttaa ccccaactgt cgggtatatg tctgccagtc acggcgtgac
    14101 gcatcatgta ataacgtatg tttagccaga tcttccgggg tcttcaaggg tttttcgcct
    14161 gtcagcagta gcggcgaaca caccggcaat aaatattcgg cgtacagttt ttccacccgt
    14221 agccccggcc agttgccccg accataaaat atcgccacat caacatcatc cgccagctta
    14281 tcttcctgac gatcaaccgc ctggattcga acgtcaattc ccggataagc tgaattaaag
    14341 ctggaaagtc gcggaaccaa ccaatgaatg gcgaaactgg ggagtaaact gaccgtcaac
    14401 gcccccttgg cgctacgggc ctggagttta cgcgtcgctt cggttaattg cgaaaatatc
    14461 tctttgatat cgaggaaata gctttgccct tcctcggtca gcaggagtga acgattacgg
    14521 cggcggaaca gttttagccc caaaaaatcc tcaagagact tgatttgatg acttactgcg
    14581 gcctgggtca cgaaaagctc ttctgctgcg cgagtgaaac ttaaatggcg tgctgcggca
    14641 tcaaaaactc gtaaggcatt tagcggtggt aatcgtttag acatggcttt taaactttga
    14701 tgttaaatta atttaacaat tagatcacac tgtgtaacct attagttttt ttaatctgag
    14761 ccattataaa ttgtccgttg agcttctacc agcaaatacc tatagtggcg gcacttcctg
    14821 agccggaacg aaaagtttta tcggaatgcg tgttctggtg aacttttggc ttacggttgt
    14881 gatgttgtgt tgttgtgttt gcaattggtc tgcgattcag accatggtag caaagctacc
    14941 ttttttcact tcctgtacat ttaccctgtc tgtccatagt gattaatgta gcaccgccta
    15001 attgcggtgc ttttttttac cttgcgatcg cgaattactg atccagttcg accatctctt
    15061 tcacgtcagt acggttgatc tgctgtttgt tgccattagc gtctttatac gaaatcatac
    15121 cggtatcgtt atcagtctgt ggtttgccgt cagagacgat ggtacgtccg tcattggtgt
    15181 gcatcacata gttcgaaccg gaacaggcgc tcagggcaaa agtcagcata caggcagaaa
    15241 taattgcggc agtctttttc atcatcttct ccttaaagcg attaataaat cttaatagta
    15301 ttgattaaca ggctaaaatt aacgcctaac actattcagc atatgttact tacgcggctt
    15361 cgccaggata tccagataat tctgatggtt agcactctcc ttgtatcaaa gtgaattttg
    15421 cgtcacgatc ggtgcatcaa gccgaggagt accatgaacg tttttaatcc cgcgcagttt
    15481 cgcgcccagt ttcccgcact acaggatgcg ggcgtctatc tcgatagcgc cgcgaccgcg
    15541 cttaaacctg aagccgtggt tgaagccact cgacagtttt acagcctgag cgccggaaac
    15601 gtccatcgca gccagtttgc cgaagcccaa cgcctgaccg cgcgttatga agctgcacga
    15661 gagaaagtgg cgcaattact gaatgcaccg gatgataaaa ctatcgtctg gacgcgcggc
    15721 accactgaat ccatcaacat ggtggcacaa tgctatgcgc gtccgcgtct gcaaccgggc
    15781 gatgagatta ttgtcagcgt ggcagaacac cacgctaacc tcgtcccctg gctgatggtc
    15841 gcccaacaaa ctggagccaa agtggtgaaa ttgccgctta atgcgcagcg actgccggat
    15901 gtcgatttgt tgccagaact gattactccc cgtagtcgga ttctggcgtt gggtcagatg
    15961 tcgaacgtta ctggcggttg cccggatctg gcgcgagcga ttacctttgc tcattcagcc
    16021 gggatggtgg tgatggttga tggtgctcag ggggcggtgc atttccccgc agatgttcag
    16081 caactagata tcgatttcta tgctttctct ggtcacaaac tgtatggccc gacgggtatc
    16141 ggcgtgctgt atggcaaatc agaactgctg gaagcgatgt cgccctggct gggtggcggc
    16201 aaaatgattc acgaagtgag ttttgacggc ttcacgactc aatctgcgcc gtggaaactg
    16261 gaagcaggaa cgccaaatgt ggctggtgtc ataggattaa gcgcggcgct ggaatggctg
    16321 gcagattacg atatcaacca ggcagaaagt tggagtcgca gcctggcgac gctggcggaa
    16381 gatgcgctgg caaaacgtcc tggcttccgc tcattccgct gccaggattc cagcctgctg
    16441 gcctttgatt ttgctggcgt tcatcacagc gatatggtga cgctgctggc ggagtacggt
    16501 attgccctgc gggccgggca acattgcgct cagccgctac tggcagaatt aggcgtaacc
    16561 ggcacactgc gcgcctcttt tgcgccatat aatacaaaga gtgatgtgga tgcgctggtg
    16621 aatgccgttg accgcgcgct ggaattattg gtggattaat gacgaacccg caattcgccg
    16681 gacatccgtt cggcacaacc gtaaccgcag aaacgttacg caataccttc gcaccgttgt
    16741 cgcaatggga agataaatat cgccagttga tcatgctggg gaaacagctt ccggctttgc
    16801 cagacgagtt aaaagcgcag gctaaagaga ttgccggatg cgaaaaccgc gtctggctgg
    16861 gatataccgt gactgaaaac ggtaaaatgc acttctttgg cgatagcgaa gggcgcattg
    16921 tgcgcggcct gctggcggtg ctgttgactg ccgttgaggg gaaaaccgcc gccgaattgc
    16981 aggcacagtc gccgctggca ttgtttgatg agctgggatt gcgtgcgcaa cttagcgcct
    17041 cacgcagcca ggggttaaat gcgttaagcg aggcgattat cgctgcgacg aagcaggttt
    17101 aagcatagaa ccgggtcgga taagacgttc gcgtctcatc cgacctgatt gtttaaccct
    17161 gacgcgccgc tttcgccatc atcttcttca gcgcatgaga aaccgcaaca aacccaaagg
    17221 tggcggtcac catcgttgcc gcgccaaatc cagatgcaca atccatccgc ttcggccctt
    17281 ctgccgtggc tttcatcgca cacacagtac cgtctgactg cgggtacacc agcgcttcag
    17341 tagaaaacac gcaatcgacg ccgagcttac ctttactgtt tttcactacg ccaaaatcgc
    17401 ttttcaggcg ctcgcgcaac ttcgccgcca gcggatcctg aatcgttttc gccagatcgg
    17461 taacctgaat ctgcgtcggg tcaatctgcc cacccgcgcc accggtcgtg accagcggga
    17521 ttttattgcg ccgacaataa gcaatcagcg ccgctttggg ccgtacacta tcaatggcat
    17581 caatcacgta cgaataaccc acgctcatat actgcgctac gttatccggc gtcacgaaat
    17641 catccaccac cgttacacgg cactccgggt taatctggcg aatacgctcc gccatgactt
    17701 ccgcttttgc cagcccgacg ttatcgcgca gagcgtgaat ttgccgattg gtattggtga
    17761 cgcacacatc atccatatcg ataagcgtga ttgcgccaat ccccgtgcgc gccagcgctt
    17821 ccgccgccca ggaaccgaca ccaccgatac caaccacaca aatatgcgcg tcagcaaaca
    17881 gttgcaacgc tttttcacca tacagacgcg ctgtgccacc aaaacgctga cgccatgcat
    17941 cactaattac cacagacata gcacctcaga attaaaaagg gtgaggttat ccctcaccct
    18001 gtcgtatccg taaaaacggt atacagaata acacatcagc cgctaaagac gttacctgcg
    18061 cccggggcgg ttttcagcac ccagacacgt ccatagtggt tgtaccaacc tgcgcggtgt
    18121 ccggcttccg gcccgatccc ttgatagata tcgaagtgtt ggcctttgat tgcaccaccg
    18181 acatccagcg ccaccatcag acgcagttcg tactgaccat taaatttgcc gttattatcc
    18241 agcaacggca cttctgccag caaggtagta cctggcggaa taatggaacg atcagaggca
    18301 actgacgcgc gaccaaccag cggcaccgca cttgcccctt tcaccggtgc aaaagattgc
    18361 ggtttaaaga agacgaaaga cgggttctgt tccagcagct cgcgaacctc ggcttcactg
    18421 tgtgtttcgc cccagtgacg aatcgcctgc atcgacatat cttctttttt cacttcgcca
    18481 cggtcgatca gcaccttacc aatgctgcga taggcatgac cgtttttccc tgcatagctg
    18541 aaaaagttaa gcggactgcc atcaccaaag tcgatatacc cactaccctg cacatccata
    18601 atgaagttat ccatcaggga gttactgtaa gcgagaatat atttatcact caatgccccc
    18661 gcgtagatct ccgcacgaga cggcagacga ccacgttttg gcggcatacg gtaaatagga
    18721 tactggaact cgccctggcg ggtatggcgc gcctgaatta ccggcgtgta atagcctgta
    18781 aactgcacgt taccatagtt gtcggcacct tccatctgcc aggcatcaat gccgaactgg
    18841 cgcatattgc gggtatcacc gcctgcgcgc agccactctt gcaccgcgtt ataaacatta
    18901 ctctggttgc catacagacg cggtgacgaa ttacggatat ggttaatttg ctcggcaaaa
    18961 tcaccggcgt taatcggcgc gccaacggca tctggctggt tcaccagaga gaaaggctgg
    19021 gtaaatttcc cgtctttata ttgctgtccg cgatcggttg gtttggaaga gcaggcggca
    19081 agcattgcca caaccgtgcc cataagaagg tactttaccc aacgtccttt cattgttctc
    19141 ttcttcaggt taaaaaataa ggcgcaacga agataacaaa ccgccgcgtt taatgaaatg
    19201 agcagcctct ccctgacgca aattttgcac aaaaaatagg ctttagtgat ttgtttttgt
    19261 tcaaaatcat gccagatccg tgatcggggt aaaaaaaagg ttgcatgaaa acgcgagcgg
    19321 agtatagtgc gcatccacgg aggcggggtg gagcagcctg gtagctcgtc gggctcataa
    19381 cccgaaggtc gtcggttcaa atccggcccc cgcaaccaat tattgaacac cctaacgggt
    19441 gtttttttgt ttctactctc ccataaaaaa agcgccattc agcgcctttt tatcatcccc
    19501 ttctcgccag cgttgcccca tcggcaaaat acgctttaat ccccgcaaga atagactccg
    19561 caacttcctg ctggaaagtc gccgttttca gtttacgctc ttcctcaacg ttactgataa
    19621 acgccgtttc gaccagaatg gagggaatat ctggtgcctt tagtacggca aacccggcct
    19681 gttcaacttg atttttatgc agcttgttga ttttaccgag cttattcagc actgctttac
    19741 caaacttaag gctgtcagca atggtcagcg actgcaccat atcgaacatg gtgtggtcga
    19801 catagcggtc accgcttttg ctcacgccac caatcaagtc cgaggcgttc tgggtttgtg
    19861 ccagatattt tgccgcagta ctggttgcac ctttggttga gagcgcaaac accgaggaac
    19921 cgctcggctg gcgactggta aaggcgtcgg catggataga gacaaacagg tcagcacgct
    19981 gtttctgggc ttttgctacg cgcacttgca acggaatgaa gatgtcttca ttgcgcgtca
    20041 tgtacacctt catattgccc tctttctcga tcagagagcg caaacggcga gctatttgca
    20101 ataccacatc tttttcgcgc gttttgtatt tccccaccgc accggagtct tcgccaccgt
    20161 ggccagggtc aagcataatg acaatcggac gatcgcgccc tgctttacct ggttgtggac
    20221 cactttgcgc tggcggcacc tgcttttcga ggtcgccttt gttgtaatcc tccagcagcg
    20281 ccagcagcgg gtcctgcata tcctgtgcat tggccggata gaggtccatc accagacgct
    20341 ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg ttttacgttt tgctttaatt
    20401 caaaaaccat ccgtacggtt tgcgggtcaa attgcccgac gcgcgccgac ttgatgaacg
    20461 ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac cgagttcagg tttacatctt
    20521 cgatatccac caccacgcgt tcagggttac tcaacgcgaa ctgcttatat ttcagctgac
    20581 gatttgattc taccgtcacg cgggtgtagc tggacgcagg ccagacgcgc accgccacga
    20641 cctggctaac cgcagccagg ctgacctgac ttacgctcaa tagccacata gcgcccgcgc
    20701 cttgcagtaa acgacgacgg ctgattgcag tgttggatcc tgacatgcct ctcccgagca
    20761 aaagaaatct aagctgtgta acaagtaaac gactaatttg accggaaact ttagcgaaag
    20821 acgcataatc tgtcatctaa taaacggtaa acattctttt tatattcacg gcattactga
    20881 taaaaaagtc gctctcgcat aaaatttaca cttgcaccct gcgaaaaaac agaataaaaa
    20941 tacaataatt tcgaataatc atgcaaaggg gtgtgccgtg gtaaaggaac gtaaaaccga
    21001 gctggtcgag ggattccgcc attcggttcc ctatatcaat acccaccggg gaaaaacgtt
    21061 tgtcatcatg ctcggcggtg aagccattga gcatgagaat ttctccagta tcgttaatga
    21121 tatcgggttg ttgcacagcc tcggcatccg tctggtggtg gtctatggcg cacgtccgca
    21181 gatcgacgca aatctggctg cgcatcacca cgaaccgctg tatcacaaga atatacgtgt
    21241 gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg ggaacattgc aactggatat
    21301 tactgctcgc ctgtcgatga gtctcaataa cacgccgctg cagggcgcgc atatcaacgt
    21361 cgtcagtggc aattttatta ttgcccagcc gctgggcgtc gatgacggcg tggattactg
    21421 ccatagcggg cgtatccggc ggattgatga agacgcgatc cgtcgtcaac tggacagcgg
    21481 tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact ggcgagagct ttaatctgac
    21541 ctcggaagag attgccactc aactggccat caaactgaaa gctgagaaga tgattggttt
    21601 ttgctcttca cagggcgtca ctaatgacga cggtgatatt gtctcagaac ttttccctaa
    21661 cgaagcgcaa gcgcgggtag aagcccagga agagaaaggc gattacaact ccggtacggt
    21721 gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc gtgcgtcgct gtcatttaat
    21781 cagttatcag gaagatggcg cgctgttgca agagttgttc tcacgcgacg gtatcggtac
    21841 gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca acaatcaacg atattggcgg
    21901 tattctggag ttgattcgcc cgctggagca gcaaggtatt ctggtgcgcc gttctcgcga
    21961 gcagctggag atggaaatcg acaaattcac cattattcag ctcgataaca cgactattgc
    22021 ctgcgccgcg ctctatccgt tcccggaaga gaagattggg gaaatggcct gtgtggcagt
    22081 tcacccggat taccgcagtt catcacgggg cgaggttctg ctggaacgca ttgccgctca
    22141 ggcgaagcag agcggcttaa gcaaattgtt tgtgctgacc acgcgcagta ttcactggtt
    22201 ccaggaacgt ggatttaccc cggtggatat tgatttactg cccgagagca aaaagcagtt
    22261 gtacaactac cagcgtaaat ccaaagtgtt gatggcggat ttagggtaag cgatgaaaat
    22321 cgccggatgc ggcaggcgta acactcgtgc gtcgcatccg gcaattacgt ttatccccgc
    22381 gaactaaaca acgccgccag accactgcgc cgctcagtac gagtggcgat tgccgcactt
    22441 aatatacgct catcggcata cagcgacaga cggcgacgcg cgcgggtcac cgcggtgtat
    22501 accagctctc gcgttactat cggcgtgcgt tggctcggta aaatcaacgc cgcatggtcg
    22561 aactccgatc cctgcgattt atgtaccgtc atcgcccacg tagtttcgtg ctctggcaag
    22621 cgactcggtt gcacagactt aatattgccg tccggcatcg caaaccagac gcgcgtcccc
    22681 tgcccgcgat ccagcgcaat accgatatcg ccattaaaca acccaagcgc actgtcatta
    22741 cgggcaatca tcaccggtcg accttcgtac caacgagagt gcgaatgacg atgaattttg
    22801 cgcttctgtt gcataaactg ctcaattcgc tcattcagtc cagccacgcc aaatggccct
    22861 tcccgcaggg cgcacaaaag ttggtactca ttgaacgcct gaatgattaa atccggctcg
    22921 gcacgcgctt gcagcagatc cagataacgt ccataacccg caagagcttc ctcaagcatc
    22981 gcaatataat cttcgccgct ctgtaaaagc cgtttttcaa tatcagtaaa atcctgctga
    23041 aaaacggttt tcactgccgt tttatcacca cggttgatcg ccgcagctaa ctgaccaatg
    23101 ccagaatcgc tgccgaaacg atagcttttt tgcagcaggc agagactgtc gcgcaaagat
    23161 gccgcttctg tgccggttcc tgccggaacg tgagttcccg tcaggcggct tagctgcccg
    23221 gcacgctcgg cggtaaagcc ggcattggca taagcgcaga tatcgcccag cacagcccca
    23281 gcctcaaccg aggccagttg atcacgatcg ccgagaaaga tcactcgcgc atgatcgggc
    23341 aaggcgtcga tcagtctcga catcataggc agatcgatca ttgacgcttc atctaccacc
    23401 agcacatcaa gatgcagcgg gttaccggca tgatgacgta aacgctggct acccggctgc
    23461 gcgcccagca atcggtgcaa agtgctggca tcttccggaa tgcgtttctt ttgttcatcg
    23521 gtcagcggta actgtcgcaa agccttgccg agagattcgg ttaagcgcgc ggcagcttta
    23581 cccgttggtg cagccagacg gatacggcag cgttcgccgt cggccatttg aattaacgct
    23641 gccagcaact tcgccacggt ggtcgtttta ccggtgcctg ggccgccgga aatcaccgag
    23701 atccgccgcg tcagcgccac tgctgccgcc actttttgcc agttaatttc atcgcttact
    23761 ggaaaaagtt tgtccagggt ttgcgccagt agagcttcat caacctcaat ggcatgattc
    23821 acctcattga agaagcgcgc taccgtccgc tcgttacacc acatgcgatt caaataaaga
    23881 cgatcgccac agaggatcat cggcgtgggt tcatctcccc tgctgaccgc ttgagaagcc
    23941 agcaagcatt cttcccaatt ttgtagctca ccgatttcac tgacacaggt cgccaacagc
    24001 ggatgcgacg cctcgttatt ttccagtcgt gaaagcggca aacagacgtg tccctctccg
    24061 gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag gatgttcatc tcccgccacg
    24121 gtcagggcaa attgcacatc cagcgggcgt agctgtttgt gctccacagc ttccagtaat
    24181 tgcttttgca atttcattac gcctcctcca gggtcatacc ggcaaacatc tcatccatca
    24241 gggcaatcaa cccggcgttg ggtcgggttg tgtaaatccc ttgttgcgga tgttctttat
    24301 caacgccacg caggaacaga tagataacgc cgccaaagtg acgatcatag tcgtaatcag
    24361 caatgcgatg gcgcagataa cgatgcagcg ccagggtata aagctgatat tgcagatcat
    24421 agcggtgtgc ctgcatcgct gctgccatag cctgttgggt gtaagccgaa ctgtcttcac
    24481 ccagccagtt ggatttatag tcgagcaggt aataacgccc ttcgtggcgg aataccaggt
    24541 cgataaagcc ttttaacatg ccgcgtacct gcatgaactc cagcggcggg cagccagcgg
    24601 atagcgggtc aaactggcga attagtgcat caagctggct ggcgatgagc ggttcgctaa
    24661 tcggcaggta aaactccatc tccacctgtt tatcgcggtc ggaaagctga ctcaggctaa
    24721 cacccgtttc attgagaggt gcctggagga cagccgtgat ccactcggtc aacaccggtt
    24781 cccactgcga ttcaaagccg ccgagttcca gtttttcctg tacccagttc gggtcaaccg
    24841 gctgggtaaa atcgaggtct tcaaacaaac tgtgcaagaa tgtccccggt gacgcaccgc
    24901 gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac gctgaccacg cctgcggcat
    24961 caacatccag ccgtggcatc agatcctgag cgataccgtg accacgctgc tgcaagccgg
    25021 agtagctggt gacacgccag ttatcgccgg gcaatcgttg tagtgtcctc gcattcagtt
    25081 ctgcagttaa agcatcatta acctgccagg gctggttatc accagtttgt gccgtttgcc
    25141 aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg aagccctgcc gcatcttgcg
    25201 gttccccttt ttgcagcaaa cgcccgagcg cactttggtg gacgtcggtg tcaccttttt
    25261 tatcgccacg acggcgcacc agcggtgcaa cgccgagact gcaatgccaa accgaacgcg
    25321 tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg ttcgacctcc gcgaggtcga
    25381 cgctttctgg cgcagcatta agatccaaaa ctgcctcaaa cgagtggcga tcgtgataaa
    25441 acgcctgatc ctggacgcgg aaattggtaa taaatggcaa ccagaccagc ggatattcca
    25501 gccctttcga tttatggatc gtgacaatct gcaccagatg tttatcactt tcgaggcgca
    25561 tttgttggct ggaggcatta ctgtctggct cgaggatatg ttgcgataac cagcgtacca
    25621 gcgcatgttc actttccagc tgcgttccgg cttcttgaag cagttcgctg atatgcaaga
    25681 tatcggtaag acgccgctca ccgcctgccg ttgccagcaa gttttctgca atgttacgcg
    25741 ccgacatcag cgcccgcagc atcggcataa cgccacgttt gcgccagatt tgccgataac
    25801 catcgaactc ttcgactacc gcatcccacg catgttcgtc attattcagc gtttcaatat
    25861 ccagcgcatt cagccccatc attgacgttg ccagcgcact gcgcagggtg ttctcacgtt
    25921 cgggcgtcat caccgcctgc aacagccaaa gcatttcctg cgcttccaga gtttcaaaaa
    25981 cactatcgcg gttcgaaagg taaacggaag ggatttccag caacgttaag gcatcgcgca
    26041 cctgggcggc ctcctggcgg ctgcgcacca gcacgctgat gtccgaagca cgcaccggac
    26101 gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg tccggcttgt agccagtcgc
    26161 ggatttgcgc agcacatacc tgcgccatgg tactttgata gtcgccaacg ccgcagcttt
    26221 cgccttccat cagccacatt ttcatcgcag gctgcgtttc acctttaaag acaaaacgta
    26281 acgcctgatt tttcccggct gatttcactg gaataaacgg tatttcgcga aacatgaacg
    26341 cgtcatcagt ctggctgaaa agcttgttca cactgttcac cattccaggt gcggagcgcc
    26401 agttggtgtc taaagtgtag tgggcgtgaa cttcactacg cgccttcata taagtgaaga
    26461 tatccgcacc ccggaatgca tatatggcct gcttcgggtc gccaatcagc aacaatgcgg
    26521 tttctggctg atggtgccag atacggcgaa aaattcggta ctgctggggg tcggtatcct
    26581 gaaattcatc gatcattgcc accgggaatc gcgtacggat cgccgctgcc aacacctcgc
    26641 cgctttcgct acgcagcgcg gaatcgagcc gacttaacat gtcatcaaaa cccaattcgc
    26701 cacggcggcg tttttcacgc gctactgttt cgcggatctc agccaatgcg cgggtgatca
    26761 ccagatcacg gatcgacaat ggttctgcaa gcagttgatc gatcgcctca aacagtggat
    26821 gtcgcggggt ttcccccccg gctttcgtgc gatcttctaa gaaacgctgg gagaattttt
    26881 ccagcgactc cggcaactga taactgtttg tctcttcttc tgcccaggcg ctgatcttgt
    26941 cgatccattt agcctgattg ctacggttaa acttgcgtcg atcaatacca gaagattcga
    27001 tcagcgcatc cagttcaccc actgcgtcgc gccactgctg ttttaccgta tcaatacgcg
    27061 ccacaatttg cgcgtgacgg gaagctagcg tttcatcatc gggcggcggt gctttgataa
    27121 ccggcgcttc gccttgcaga taacgattaa tatcgcgcag caacgcctgc ggccctttcc
    27181 aggtttcaaa gacgacctgg gctatttcac gcggcagcgg gtagcagtgg cgacgccaga
    27241 aatcggcgca ggcctggtag cgtagcagag actcatcttc aatcagctgc tgctcaaaca
    27301 gcatgccgga ttcaaaggca ttcaggttga gcatgcgctg gcaaaagccg tgaatagtaa
    27361 agactgccgc ttcatccatc tgccgttcgg ctaacaacaa ccactgcgcg gcttgcgctt
    27421 tatcgtcgat ctcttccagc aggtgttcgt acaatggatt gtcggtggtt tcacgcagac
    27481 aggcgatgcg caactcgtgg atattgctac ggatacgacc gcgcaattct gccgtggcag
    27541 cctcggtaaa ggtgaccacc agcagttctt caacggtcag cgggcgggga aaggcggcgg
    27601 aaccgcctag tccaagtaac aggcgcaaat aaagcgccgc aatcgtaaag gttttgcctg
    27661 tgccggcaga ggcttcaatc aggcgctcgc cctgtaaggg caagcgcaaa ggatctagtg
    27721 tctcggcgac atcactcatt cttttcactc atcaggggca ttgtttgctg caacgcgctg
    27781 acgttctccc acactttcca gccttcagga tgcacatatt cggctttccc gttttggctg
    27841 ccggaaatct gcgacaggat agccatgcct tgcggctcga ctaccgcctg atggaagaaa
    27901 tcggccagtt tttgcggcgt cagcagttta atctgggcca cgattttatc acgcgaatcg
    27961 aagcgcatat tgccgcgatc gaaatcttta cttaacttcg atgcttcttc gccgagcgtt
    28021 tgcggtgcct gcagcatctg ggtaattacc gcctgctgga tttgcgcaaa ctcatctggc
    28081 ttcatcgctc gcaattttgc ctctgcggtt gggaaaaacg ccttgtaacg ctcccacaag
    28141 aatgaaggct gtttatcatt gctttgcaaa aggaagccca tgccccactg acgccccacg
    28201 ctcattggaa acgcaaacac ggcatagccc aattgttctt cggtacgcaa ctgattgtag
    28261 aaccacggct gtacgatctg ccccaacaga gagctatagg ctgagctggt gtattcatcg
    28321 tagccagtcg gtacaaatac cgctgccagt gcggagtcgg tgctgttacc ggctttttca
    28381 aagatgacgg attgtttttt atcgaccact acatctttgt ttcgacacca ctctgaacca
    28441 tcagcgccca actgtttttg cacatcgcgt gccagcgttg ttgcctgggc ctcggtcatg
    28501 ttgccgataa ccataaactc tggtcgagcc cctgatttta aggcgtcgcg ataagccagc
    28561 acctctttca acgtaatgga gggcaaaatt ttacgccgtt catctcgcga gaagtacggc
    28621 acttgcgaga gcatctgcgc gggcataatc gcctgctcaa acgctttacc cttttctgcg
    28681 gaatccatca tctggttata ccaggacttc gcctgctcaa gctgatcttc cgtagcggta
    28741 tagctaaagt acccctcgag caatgcctgg aacagctgcg gcagacgctg ggggtaacca
    28801 ttagcattaa ccataaggcc gttgttagcg ttggtggaaa aacttatgcc accaaccgac
    28861 gcctggttgc ttaactgatc aagcgccagc cctgcgagat aatcattgag cgcaaacatc
    28921 acctgattgc gggcgctgtc catggctttc ggattacgca aaatcaggct gacatcagct
    28981 ttgggttcgc tggaaaaata acggcttggc gcatacacca cgcgcagatt cgactcatca
    29041 acaatcagct ctggatggtc atatttcttc tctgacttaa tcagcgagaa atcgtcagga
    29101 atataggggt taagctccgg taaggagagc gcaatattgg cagctttttg ctgccagtca
    29161 gcgaaagttt gttcgctgat tttatcgacc tgatacggcg catcgacaaa ataagccgtt
    29221 ttattgtgcg gctctttcgg gctgatatac cagatacgcg cattctgcgg cgtcatcatc
    29281 gccagacgtt cttttactgc tttagcatcg taccgatcgg caatattgac tgcatccagc
    29341 gtatgctcaa ccggaacgcg aatcatggta tctgccagcc attcgacgta atccatatca
    29401 cgggtgatcg acggataacg gaagtcgata tccagcacat tcgccagttc atcgaagtat
    29461 tgtttatcaa tgcctttttc acgtaacaga ttgagatagc taaaaattgc cgccacaacc
    29521 tgatcgcgat tagccaggcc tttatcggtt aaagacgcag agatcgctaa tacgccgctg
    29581 ttgccgttga cgataggatc ggagttggcg ctaatgccct caactaatcc ctgcttttgc
    29641 agccagtcgg aaagtgtacc tgggctgcga ttaccaatca gataggtaat caattcatcc
    29701 gttttactac ggaacttcgc tgagttgtta tcgatgcgaa actcaacgcg cagaacttta
    29761 cgcggcattg ccgggacgta atgaatgata atgccctttt gcgcgtcggt gactaccggc
    29821 acggtgattt ccggtttttt gctctctttg ttcggcacgc gaccaaaggt gtccgccgcc
    29881 atttttgcca actccggcag cggtttattg ctgtaaataa ccgccttcat cagattggcg
    29941 gaatagtact tctcgtggaa atctttcagc gcctgctgca ccggattacc aggtttatcg
    30001 cttaaagttt cgaggttacc accagaaaac tttgaaccgg ggtgtgccgg gttaatggtt
    30061 tctgcgctga cctgtgccat gcgcatcccg tcacgcgtac gcgccatggt taattcagcg
    30121 ttcaccgcat tacgctcacg ttcggcatat ttcttgtcga gcaaaggttc agcaatagca
    30181 tcggccaggc ggtctaccgc accaggcaag gcgtcgttct caacttccag atagaaagcc
    30241 gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt gcattttgag atattcggcc
    30301 agactgtcag cctgcgggta ctttttcgac cccatcagac tcatatgttc aaggtaatgt
    30361 gccagcccct ggtacgcctc gggatcttcc agcgacccaa cgggcaccac cagcgccgag
    30421 agcgatttaa ctgcctgcgg atcagaaacc agcaagacca ccataccgtt atccagacgt
    30481 atagcctgat actggcggtt atctttatca cgtttacgga tggtttcctg aatcggctgc
    30541 catcccgttt ctgcctgact taagggtgcc caaagggcaa ctaacaacaa taatgctttg
    30601 aaccaggtgc tgcggggcat tctcggacct cataatcaac ttaattttct gtccagattc
    30661 aacacgttaa cgcaaatcat ctgccagaat ttaatcttgt gctgcacgag tcagcctatg
    30721 tttatataac catcagtccg tgactggtgc gcatcataaa gtaagcggat agattgcgca
    30781 atttttatac agcactcatg actgattaaa gcgaaacagc ggtaacagga aacgttgcga
    30841 ctgttcaacg atggcctcca ttgtctctgg tgttaattgc cgccagagcc tttgatacca
    30901 gatatcatca ccttcgccac gcaccatcat gttgccttcg taagcctgaa ggaatttcgt
    30961 acgggctttt tgcaacgtgg aatcgtcatc cagcatggca tcgttttgcg cgtcataaca
    31021 ggtttttagc cacgcgccgc cactttcagg taacaccagc aatggcgcgg acatcccttc
    31081 acgatacccc tcaatcagtt gtgagaggta atgcaaagcc tgttcggctg caagcggcgg
    31141 aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt tcaccattac caccgctggc
    31201 acagtagaca aggtgttcca gccaaagttg cattccctgc gccacactta ataaagaggg
    31261 acgccagcgc aacaggccat ccggctgcac ctgcggcaac cagccagtta tctgcacacc
    31321 gttgcaggcg agatcaattt ccatactctg ccccggctgg cgacaggcaa tgactctgtc
    31381 ggcaagctgc tgcatctcct ggcactgagt ttcccagaaa atttcaccaa aagcgccata
    31441 cggtaaatcc cctgccgccc ggaagcgacg gaacaagcgt tcggcatcat cctgctcaac
    31501 cagtgcattc aataactgct gattgatttg ataacggcta agcccttcca gaataaatgg
    31561 ctcggtgtcg gggatttcgc tgtcttcagt acggaagttc acctgcaaac gcatctggaa
    31621 gaacgcccgc accggatgtg cccagaatcg ttgtagcgtt tccagcggca cggtttccgg
    31681 taaggtaaac ggcagcggct gaacaaattc agaatgtgct ttaccagcct ggctggccgc
    31741 aggtagccat tcacgagcat agctttgtcg ttcgcctggc tgatagtttt gtggatcaaa
    31801 cggcattcgg gtatggaggc aagtaagatg cgcttttacc cttgcctcgc tttcatcaca
    31861 gttgagcgct tcatcgcccg gtagataatg actttgcccg atgtagtcga tcagttcctg
    31921 caccagtacc gacgggaaac gttcactgtt atcctgaatg gaacgcccga tatagctgat
    31981 atagagtttt tgctgcgcgg aaattaacgc ttccaggaac agatagcggt cgtcatcgcg
    32041 acggctacgg tcgccacgct tcggtttctg gctcatcagg tcaaagccca atggcgcaag
    32101 ctgacgtgga taaacgccgt cgttcattcc cagcaggcaa accactttga acggaattga
    32161 acgcattggc atcagagtac aaatgttaac cggtccggcg agaaaacgct ggctgatacg
    32221 ttcttgatcc agacgctgtg ccagttcatc acgcaatagt gacagcggca ccgcgtcgcc
    32281 atactgcgca cctaaacctt cggcgataat cgcctgccat tgttgttcga tcagcgtcat
    32341 cgccgcttcg gtttccgcat ccggcaggaa gaaggcgctg agcatatcgc gacaaaccgg
    32401 caaccactct tctagcggac gctcctgtgc cagcccgcga cgccagatgt ttagctgcat
    32461 tagcagtgaa gccagatgcc ccaccagttc tgcaattaag ccgctcgatt catcataagg
    32521 tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg tagcccagca acatacgcgt
    32581 caggccaaat cgccaggtgt gttgtccggt ggcggggagt tccagctcgc gaacgttgtc
    32641 gtcatctatc ccccaacgaa tgccggattc gttgacccac tggcgtaaat aacgcagccc
    32701 ttcttcggtg atgtcaaacc gcgccgccag caccggcaca tccagtaatg ccagcacgtc
    32761 ttccgacaca aagcggctgt ctggcaacga taacaggctg ataaacgctt ccagtaccgg
    32821 atgcgactgc cgcgcccgac ggtcggaaat ggcgtaaggc aggtaacgat ccgcaggtgc
    32881 gctaccaaac acagcccgaa taaacggact gtagctgtcg atatcagcca ccatcacgat
    32941 gatgtcgcgc ggagtaagtg tcgggtcttc ttccagcatc gccagcaggc gatcgtgcaa
    33001 aacttcaact tcacgctgcg ggctatggca aacgtggaag gtgatactgc tatccagtgg
    33061 atcaagcggg cgtttgttat cgctacggga aaactcttcg atgttcacac cagcaacggc
    33121 acggttttcc agttccagaa tgtcagactg aatgttatgc agcaggttat ctggcgtgac
    33181 atcgacaaag gcgtccagct cctggctgct ctccaggtca gaaaggagat aaatgtagtc
    33241 gcgcccaagc ttaccccatg aagccagcag cgggttgccg acatcctgtt caccatcgct
    33301 gttaaagagc tgcccggcat tttcgctatc acgaaatagc ggcaattcgc gatcttcaaa
    33361 actgtgtcga cgctgacggg tcagcagttt cgccagataa gcaggatctt taatatcgcc
    33421 ccagtaataa cggcaggggt tggtaaacag gagatggatt tcaatatgtt tacccagcgc
    33481 ctgtagcgcc tggagataaa caggcggtaa cgcggaaata ccgcatataa agacgcgcga
    33541 aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt tcgataaagc gctgatagag
    33601 attggcgcgg tgccagcgcg gttgcccgag ttgatgggta tattccacca gcgccttcca
    33661 caacggggct tgccaggcct gtgcttctcc cagcccttca accaaatgtc ctgtttccca
    33721 ctgtgccagc cagtccggac gatagaccag atactggtca aacaggtccg ccgcttttga
    33781 ggaaagctgg aacagttttc gcttgtcgct atcgtcagtc agataatgcc gcaacagggt
    33841 aaagtcttcg cgctccagca attgcggcag cagagtcatc agtttccagc tcatgctctg
    33901 tttgttaaag gcgctctctt tggggatttc cggtaacacc cggacgaaca tatcccagat
    33961 aaagctcgct ggcagcggaa aatcaatgtt tgccgcaata ctaaactttt gcgacagggt
    34021 catttgcagc cactgcgcca taccggtact ttgcaccaga atcatctctg gttcgaaagg
    34081 atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac gcttccagca cgtccagacg
    34141 attggaatgg tagaccctta acatagcggc tcctgactac tgacgattcg ggcaatgcag
    34201 gcgggtcatc tcgccttctc tgcccccggg tgaaactagc gtaacgctga tgctgacaca
    34261 tcccgcctgc gatgtctgca ttcggttgac ctgccagttg gcaggtggcg aaatcgcgcg
    34321 cagttgcgtt tgctgccaac catgccgcca gagctgttgg tactggtttc tgctggcgag
    34381 actgttcatt aatgttcgct ggatacccga taacgcagtg acaatcatca ccatcagcac
    34441 catcgcaacc attacctccg gcaggctaaa gccttgttga ttcttcaggg aagctgacat
    34501 aacgccctct ctttcagcgg acaaaaatcg ctccagccgc gtggcgaaaa aacaatgttt
    34561 ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt cataaccggc aaccaataag
    34621 gcttcattat ctgccagtaa acgcaaacaa acccgggcat cggttccagc gtactgcgag
    34681 cactgaactg cgggctgcgt ctgccaggag tgcattttcc cccacgccag cgccgactga
    34741 acgatggcct ggcggcgcaa tgactgactt tctatgctca cgcgagaggc aaaactgcga
    34801 tcctgctgac tcattccttg caatagcaag ctacccaaaa ccagcagcat caggaccaga
    34861 gccagtgacg aaacaccttt ttcgcggttc acaggttgaa tcctgtcacg ctatagctgg
    34921 catccaccac ggtttgcggt tcagacttac tggcagcacg catattaacc gtcaacaccg
    34981 gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt gtcgataatg atggcatccg
    35041 gattagtgac tttatcccag cccttacctt cacaggatgt cgcaccgcgt agcgtttcca
    35101 gcacatgctc cttcagacga aatccaatct ggtcagactc ttttaccggt tcgcgatccc
    35161 agataccgtt actgttcgca tcccactgca caatgataca gtcaccctgt ccgacaattt
    35221 ccagcccttc gccggtacag ctgccatgac aataacccgc cctctggaga tgcttcgcga
    35281 cggtaaatac ccgcagccag atttcatctt ccagcgccag cttacgggtg ctcgttaaac
    35341 tttcacgctg taacgcaggc agaaagcgtg cagcccccag caacaatacg ctactgatcg
    35401 ccatagcaat caacacttcc agcagagaaa aaccttgctc ttttacaggc atccttctgt
    35461 ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa accaccagcc accactcgcc
    35521 cgttgagttt ttgaagcgaa tatgcccggc ccatgcggta ttgcgcaggc caaagaaagc
    35581 aagcgaaggt gtcaggtcgc tcatttagac ttcgggccag cgtggcacaa agaccaatgg
    35641 tgaactgcca tgacaggtat tggccccagc agcggaactc acaaggcacc ataacgtccc
    35701 ctccctgata acgctgatac tgtggtcgcg gttatgccag ttggcatctt cacgtaaata
    35761 gagcaaatag tcccgcgcct ggctggcggt ttgccatagc cgttgcgact gctgccagta
    35821 ttgccagcca tagagtccac ttgcgcttag catgaccaaa atcagcatcg cgaccagcgt
    35881 ttcaatcagc gtataaccac gttgtgtttt catgccggca gtatggagcg aggagaaaaa
    35941 aagacgaggg ccagtttcta tttcttcggc gcatcttccg gactatttac gccgttgcag
    36001 gacgttgcaa aatttcggga aggcgtctcg aagaatttaa cggagggtaa aaaaaaccga
    36061 cgcacactgg cgtcggctct ggcaggatgt ttcgtaatta gatagccacc ggcgctttaa
    36121 tgcccggatg tggatcgtag ccttcaatct caaagtcttc gaaacggtag tcgaagatgg
    36181 attcgggttt acgtttgata atcaacttcg gcagcggacg cggttcgcgg cttaattgca
    36241 gatgagtttg atccatatga ttgctgtaca ggtgcgtatc gccaccggtc cagacaaaat
    36301 cacccacttc cagatcgcac tgctgcgcca tcatatgcac cagtaacgcg tagctggcaa
    36361 tgttgaacgg caggccgagg aagacgtcac aggagcgctg ataaagctgg caagagagtt
    36421 tgccgtctgc cacatagaac tggaagaatg catggcacgg tgccagcgcc attttatcca
    36481 gttcgcctac gttccacgct gaaacaataa tgcggcggga atccgggtcg tttttcagct
    36541 ggttcagtac cgtagtgatc tggtcaatat gacgaccatc tggcgttggc caggcgcgcc
    36601 actgtttacc atacactggt ccgaggtcgc cgttttcatc ggcccattcg tcccagatgg
    36661 tgacattgtt ttcgtgtaga taagcaatgt tagtgtcgcc ctgcagaaac cacagcagtt
    36721 cgtggatgat ggaacgcagg tggcaacgtt tagttgtcac cagcgggaat ccatcctgca
    36781 ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc ggttccggta cggtcgtttt
    36841 tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa atactgtttc atggttcctc
    36901 aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac catcatgatc acacccgcga
    36961 caatcatcgg gatggaaaga atttgcccca tgctgatgta ctgcacccag gcaccggtaa
    37021 actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg aaacgcgccg tagccaatca
    37081 ggaacaagcc agagacagct cccattgggc gcggtttacg aatatacagg ttgaggataa
    37141 taaacagcac cacaccttcc agcagcagct cgtaaagctg tgatgggtgg cgcggcagca
    37201 caccgtaagt gtcgaaaatg gattgccact gcgggttggt ttgcagcagc aaaatatctt
    37261 ctgtacggga gcctgggaac agcatggcaa acgggaagtt cgggtcaacg cggccccaca
    37321 attcaccgtt aataaagttg cccagacgcc cggcaccaag accaaacgga atgagtggtg
    37381 cgataaaatc agaaacctgg aagaaggaac gtttagtacg gcgggcaaag ataatcatca
    37441 ccacgataac gccaatcagg ccgccgtgga aagacatgcc gccgtcccag acacggaaca
    37501 gatacagcgg atcggccata aactgcggga aattgtagaa cagaacataa ccaatacgtc
    37561 ccccgaggaa gacgccgagg aagcccgcat agagtaagtt ttcaacttca tttttggtcc
    37621 agccgctgcc cggacgattc gcccgtcgtg ttgccagcca cattgcaaaa atgaaaccca
    37681 ccagatacat caggccgtac cagtgaagcg ccacgggtcc tattgagaaa atgaccggat
    37741 caaactccgg aaaatgcaga tagctactgg tcatctgtca ccacaagttc ttgttatttc
    37801 gctgaaagag aacagcgatt gaaatgcgcg ccgcaggttt caggcgctcc aaaggtgcga
    37861 ataatagcac aaggggacct ggctggttgc cggataccgt taaaagatat gtatatgatc
    37921 cgcgctataa ccctccgcga atcagcccgc ccatgccgcg acgctccata aaggctgcaa
    37981 cctgatggcg aacttcggtc gccagttgcg cttccagact acgctgcgca agattttctg
    38041 cttcggcata atcaatgcgc cgcagcaggt attttacccg cgctacagaa cgtccgttca
    38101 tagacagatg gcgataccca agcccaatga ggattgccac gcacatggga tcgcccgcca
    38161 tttcaccgca caaacggaga tcgattccat gtatttccgc ttcccgggcg atcatcgcca
    38221 gagctcgtaa cattgcagga tgaagactgt cataaatgtt cgccacccgg gtattgttgc
    38281 gatcaacggc cagaatgtat tgagtcagat cgttggtgcc aacagagatg aaatcgaccc
    38341 gctttgccag atgcggcagc ataaatacca ttgacggcac ttccagcatg atgccgatac
    38401 gtggtttggg aatttcgtaa ccgatcatct cctcgacttc acgtccggca cgttcaatca
    38461 ggcggcgtgc ttcatcaact tcatcgaggc ttgtgaccat cggcaacaga atattcaggt
    38521 tgcccgtagc ggcattagca cgcagcatcg cccgcacctg gatcaagaag atctccggct
    38581 gatcgagcgt aatgcgaatc ccacgccaac ccaggcatgg attctcttcg ctgatcggca
    38641 tgtaaggcag ctgcttatct gctccgacat ccagcgtacg caaggtgacg ggtttatcat
    38701 tgaacatttg cagcatcccc tgatactgcg ccacctgttc ttcttccgac gggaaaccac
    38761 tttgcagcat gaatgggatt tcagtgcgat aaagaccgat gccatcaata cggctgccca
    38821 gtttttcttc atgttccggg cttaaaccag cattgagcat gacttttata cgctcaccgc
    38881 tttttaactg cgcgggtaaa ttgacgtcat cttccgccag acggctaagc tcaatctctt
    38941 cactaattag ccgctgatat tcttgcagca gtaccggctc cggatcgacc agcaattcac
    39001 cgcgatagcc atcaacgatc agcgtcctac gatgcagcac cgaaggctga atatccgcgc
    39061 ccatcacggt agggatcccc agcgcacgta ccatgatcgc agcatgggag ttggctgcgc
    39121 catctcgcac gacaacaccg actaagcgat cctggggcag ctcagcaaga gttgtcgctg
    39181 acagttcatc tgccaccaga atgaaacgtt ccggccaggc gttcggccct tgattagcgt
    39241 catcaagatg aaacagcaat cgctgaccca gcgcacgtaa atcgccagcc cgctctttga
    39301 gatagttatc gcttagcgcg gcaaactgtt cggcaaattt ttcaatgacc gtttttaccg
    39361 cccactctgc caccgagcct ttatcaacct cggcaaacaa ttcgcgacgc agccgggtgt
    39421 ccgaaagtag gtgcgagtaa agatcgaaaa tagccgccgt ttctttttgt gctccggcgg
    39481 caaagcgttt gctgtagcgg cgaaactcgt ttgccgcttc ttccagcgcc ccggtcagtc
    39541 gttcgcgttc cagagccgga tccagcgttg atgcctgata cacctgttcc attaaaggta
    39601 acgtggcatc ctgccagcct tcggcaatcg ccacaccagg tgctgccggt aatgcgcgga
    39661 ttcgcgtctg gcgatattgc ccaaacaagg cagtcaactg cgactgagaa agaatagctg
    39721 ccatctgggt ggcaagcgtc accaggaagg attcttcact ttcgtcatac tggcgcaact
    39781 ctcgttgctg taccaccagt acaccaagca actggcgacg ttgaataatt ggtacgccta
    39841 aaaacgcgcg gaaacgttct tcttttacgg aggggatgta tttgaagctg gggtgctttt
    39901 gcgcatctgc aaggtttatc ggttccgcca gcctgccaac caggccgacg atcccttcat
    39961 caaacgcgag cgttacagtg cgaccgcgtg gttttttcag cccccgggtc gccatcaggt
    40021 agtaacaacg tcgatcatga tcggccaggt agaccgaaca gacctcggta tccatcgcaa
    40081 gacagatgtc ggtaaccaga atatttaacg cctcattcag gcgtggtgcg ctggctacct
    40141 tttcgactat ttcgcgcagg cgagtgagca taattggcgt gacttaacct cttttacgtc
    40201 gataagcaga tgtgttttgt ggttttggcg tattttcctg cagtgacatc accacactcg
    40261 cgaactcttt cattacccta cggtagacat cacgtttaaa tgacaccacc tgtctgaccg
    40321 gataccagta acttacccat cgccagccgt caaactctgg tgtactgctg gtttgcatat
    40381 tgatttctgc atcgccgctc accagctgca agagaaacca tttttgtttt tggccgatac
    40441 aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa tttgtagcgc aaccagttac
    40501 gcgttgaagc aaggattcga acgtctttgc ggcttaatcc tacttcttca aacaattcac
    40561 ggtacatcgc ctgctctgcg gattctccgg ggttgattcc gccttgcgga aattgccagg
    40621 agtgctgacc aaatcgccgg gcccacatta cctgcccctg gcgattacaa atcacgatac
    40681 ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact acctcaaaat aaagctttat
    40741 atacgaatga ttgtttcata ctccaagaag acggtaaacc actctctgca gggcattaca
    40801 cactaataac aattgaataa ctcacagtta tgtgcagagt tataaacaga ggaaggggtg
    40861 gatagccgtt tttattcacc ttttctgtgg atagagttgt gaagaactac ggaattacta
    40921 cgggaaaacc cggagcattc tgaataagcc cgatatagac attttaaaat attcattatt
    40981 agtcaatgca ttacatgtaa tttgagtaat gaaaaagagt aattcgtgac ccaggtcaca
    41041 cctctcattt acgggttggc gaaagatcaa ccaatgccgt atttatccac agaatgtgcc
    41101 actaagttaa gcactgaacc actaaaaact ggagtttcgt cgcacgtcaa ggctgtaaat
    41161 ggaaacagta gtggaggttt ttcacagtta tcccagcttt ctgtggataa catggtgtaa
    41221 gatcctgttt attttcagtg accagatttg gaaaacctat tgcagtgttg cgcaactcga
    41281 ttaccggcag cctaaaaagc aatataaatc agagaattga acaacacatg tggaaaaagt
    41341 tacactgcga atattcggca cataattgct gtttgttttt taaccaaggt atcatgacat
    41401 gtcccaacct cgcccactcc tctctcctcc cgaaactgaa gaacagttgt tagcgcaagc
    41461 acagcaactt tctggttata cattgggaga actggcggca cttgccgggc tggttacgcc
    41521 agagaattta aaacgcgata agggctggat tggcgtgtta ctggagatct ggctaggtgc
    41581 cagcgcaggg agtaaacctg agcaagattt tgctgctctg ggcgtggaac ttaaaactat
    41641 ccctgtggat agtcttggtc gtccgctgga aacaacattc gtttgtgttg ccccgttaac
    41701 gggtaatagc ggggtgacct gggaaaccag ccatgtgcgc cacaagctca aacgcgtact
    41761 gtggataccg gttgaaggtg agcgcagcat cccgctggcg cagcgtcgcg taggatcacc
    41821 gttgctgtgg agcccgaatg aagaggaaga ccgacagcta cgcgaagact gggaagaatt
    41881 aatggatatg attgttctcg gtcaggttga gcggatcacc gctcgtcacg gggagtattt
    41941 acagatacga ccgaaagcag cgaatgcgaa agcgcttacc gaagccattg gtgcccgggg
    42001 cgaacggatt ctgacgctgc cacgcggctt ttatttgaag aagaatttca ccagtgcact
    42061 actggcccgt cattttctga tccagtagcc atcgctttga cctgccgctt tccgggcata
    42121 taattaccgc ttcatttttt tggcagggct ttttagatgt tatttgcatg gataaccgat
    42181 cctaacgcct ggcttgcgct cggtacgctg acgctgctgg agatcgttct tgggatcgac
    42241 aatattattt tcctttctct ggtggtggca aagctcccca cagcacaacg tgctcatgcg
    42301 cgccgtctgg ggttggcggg agccatggtt atgcgtctgg cgctgctggc atcaatcgcc
    42361 tgggttacgc gcctgacaaa tccgcttttt acaatattca gtcaggaaat ttccgcccgt
    42421 gatttgattc tgcttctggg tggcttgttc cttatctgga aagccagcaa ggaaatccac
    42481 gaatccattg aaggtgaaga agaagggctg aaaacacgcg tttcatcatt ccttggcgct
    42541 atcgtgcaga ttatgctgct ggatattatc ttcagcctcg actcggtgat taccgctgtg
    42601 ggtctgtcag atcacctgtt tattatgatg gccgccgtgg tgattgccgt aggcgtgatg
    42661 atgttcgccg cgcgctcgat tggtgatttt gtcgaacgcc atccttcggt aaaaatgctg
    42721 gcgctctctt tcctgattct ggtcggcttt accctgattc tggaaagttt cgacatccac
    42781 gtaccgaaag gttacatcta cttcgcgatg ttcttctcta ttgcggttga aagcctcaac
    42841 ctgattcgca acaaaaagaa cccgctctga tactccgtac gctctcctgc gggagggcgt
    42901 actcactcct gttaccctca cagtttaaga ttttcctgct ttcaagatat ataacgtcgg
    42961 tttataaaca gactattatc ataggtgggc caaatgagga taaccggatg aaaaaatggg
    43021 cagtaataat ttctgcagtc ggactggcgt ttgctgtttc cgggtgttcc agtgattacg
    43081 tcatggcgac caaagatggc cgtatgattt tgaccgatgg aaaacctgaa attgatgatg
    43141 ataccgggct ggtgagttat cacgatcagc aaggtaacgc gatgcaaatt aaccgtgatg
    43201 atgtttcgca aattattgaa cgttaacaaa taaggtcagc atccggctgt aatgccgatc
    43261 agttaag
//
LOCUS       DAZRUF010000010        40097 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZRUF010000010 DAZRUF010000000
VERSION     DAZRUF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40097
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.010"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="ACHXOL_000430"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="ACHXOL_000430"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR6807505.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHXOL_000431"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHXOL_000431"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR6807506.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHXOL_000432"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHXOL_000432"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR6807507.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHXOL_000433"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHXOL_000433"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR6807508.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHXOL_000434"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHXOL_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR6807509.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHXOL_000435"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHXOL_000435"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR6807510.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="ACHXOL_000436"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="ACHXOL_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR6807511.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHXOL_000437"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHXOL_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR6807512.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="ACHXOL_000438"
     CDS             6537..6773
                     /locus_tag="ACHXOL_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR6807513.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="ACHXOL_000439"
     CDS             6776..7633
                     /locus_tag="ACHXOL_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR6807514.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="ACHXOL_000440"
     CDS             7654..9645
                     /locus_tag="ACHXOL_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR6807515.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHXOL_000441"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHXOL_000441"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR6807516.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="ACHXOL_000442"
     CDS             complement(10248..11144)
                     /locus_tag="ACHXOL_000442"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR6807517.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="ACHXOL_000443"
     CDS             complement(11193..11432)
                     /locus_tag="ACHXOL_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR6807518.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="ACHXOL_000444"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="ACHXOL_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR6807519.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="ACHXOL_000445"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="ACHXOL_000445"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR6807520.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMEE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="ACHXOL_000446"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="ACHXOL_000446"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR6807521.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="ACHXOL_000447"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="ACHXOL_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR6807522.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="ACHXOL_000448"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="ACHXOL_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHXOL_000449"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHXOL_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR6807523.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="ACHXOL_000450"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="ACHXOL_000450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR6807524.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="ACHXOL_000451"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="ACHXOL_000451"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR6807525.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="ACHXOL_000452"
     CDS             complement(21570..22739)
                     /locus_tag="ACHXOL_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6807526.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHXOL_000453"
                     /gene_synonym="ynhF"
     CDS             complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHXOL_000453"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR6807527.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="ACHXOL_000454"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="ACHXOL_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR6807528.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHXOL_000455"
     CDS             complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHXOL_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR6807529.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /gene="punC"
                     /locus_tag="ACHXOL_000456"
     CDS             25360..26571
                     /gene="punC"
                     /locus_tag="ACHXOL_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR6807530.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="ACHXOL_000457"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="ACHXOL_000457"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR6807531.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHXOL_000458"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHXOL_000458"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR6807532.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHXOL_000459"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHXOL_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR6807533.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNKKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="ACHXOL_000460"
     CDS             complement(30320..31576)
                     /locus_tag="ACHXOL_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR6807534.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="ACHXOL_000461"
     tRNA            31884..31960
                     /locus_tag="ACHXOL_000461"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="ACHXOL_000462"
     tRNA            31965..32041
                     /locus_tag="ACHXOL_000462"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="ACHXOL_000463"
     CDS             32149..32454
                     /locus_tag="ACHXOL_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR6807535.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="ACHXOL_000464"
     CDS             32580..34184
                     /locus_tag="ACHXOL_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR6807536.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHXOL_000465"
     CDS             complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHXOL_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR6807537.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHXOL_000466"
     CDS             complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHXOL_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR6807538.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="ACHXOL_000467"
     CDS             complement(35794..36462)
                     /locus_tag="ACHXOL_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR6807539.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="ACHXOL_000468"
     CDS             complement(36526..37164)
                     /locus_tag="ACHXOL_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR6807540.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39279)
                     /locus_tag="ACHXOL_000469"
     CDS             complement(37177..39279)
                     /locus_tag="ACHXOL_000469"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR6807541.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(39300..39926)
                     /locus_tag="ACHXOL_000470"
     CDS             complement(39300..39926)
                     /locus_tag="ACHXOL_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR6807542.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         9616 a        10542 c         9886 g        10053 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcga
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgtg caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacgctcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggagg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc catttttagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaggg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtggca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gacgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaagcac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca cggtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacaaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagacgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatg aagttaacgt cacccgcttc
    38041 cagttgatcc cagcgaattt ccgcatactc ttcagcaggc agaacacgct tgaacacacc
    38101 tttgctgtaa caataggtaa agtcgcgatg caactgcccg tagttacacc ataggccgta
    38161 gtcatcgaac agattcagac ccaccaggtt accaatcaca cgaccatcgt ctttatcttc
    38221 aaaatctttc ggaccgttcg ggaagatggt ggtatggaca aagttggcta cacaagtgtt
    38281 accacctgtg ctgggcacgc caaactcttt cacccgagga atattcattt gggtcataca
    38341 acggatcggg caagagtggc agccactcat ttttactgtg tatttctctg ccgccggacc
    38401 taagtcaaaa acggatttat aggtacgaaa gccgaccgtg ttctgattgc ctggcggaat
    38461 ttcccccgtt tcaatcggac caccttcagc cgcgccccaa aacagccctt tacgtgctgt
    38521 ccaacgtgac ttgggatctg aatactctgc ccacgattgc ggcgtacttg gcacgacatg
    38581 gttgttattc gcaccaataa gttcagtcat catgtaatcg ttgagacgct tcatctcctg
    38641 acgatcggca atgttgaccc ctttcgtccc ttcaaccgca atcgctttca ggtttttcga
    38701 acccattatt gcgccagttc ccgcaccgcc gctgtggtta cggctattca acatgccaga
    38761 gagaggaaca aggttttccc cagcctgacc aatagccgcc acacaggttt ccggactggt
    38821 caatcgacaa atttcttccg tcgttgcgcg cgtccctttt ccccataaga aatcggcttt
    38881 ttccaggcta actttgtcat ctttaatctt cagccatacc ggtgatttcg ccttcccttc
    38941 gataataatg acgtcgtatc cagcgaattt catttgcgct gcaaaaaagc cacccatatg
    39001 ggcatcgacg actaaatttc ctttggtaaa agtagaaagt gaggtgatat ttacgcgaga
    39061 actacagggg gcaccagatc cagttaatgg gccggtagca aagactaatt tattcgcttc
    39121 atcgaaaggt ttcgtgcctg gcggtacttc gtcatacata attttgtagc cgaagcccat
    39181 gccaccgaca aaacttttaa acttactgga atcttcgagg gtaatatttc ctgtcgtgag
    39241 attgactctt aatatattac ctgtccaacc gttagccatg attttttcct ttgcaagatt
    39301 cacacagtaa tatctttcca ctcgataatt tttaacgccc ctgttgggca ggcgtttgcg
    39361 cattcaccgc ataacacaca tttcgaggat tttttacttt cggtatttac ggtggccatc
    39421 atccacgggc atgccgtagt acaggcgcta cagccaatac agcgtttatg atcgacggta
    39481 atacagcctt ctttctgctg ccaggtaatc gcaccaatcg ggcagacgtt catgcattgc
    39541 ggctctttgc attgacggca ggtgtccgcg gtatagttga gatcgccata caggccgccg
    39601 ccagagccaa ccccgttgtc gccaaagaaa taattgcgat ggattttgat acgggagaag
    39661 aatgtcccta ctgagccatc gttgaagttg gtacaagaga tttcacagcg gtggcaaccc
    39721 gtgcatcgcg ctcgctgggt caccagcacc cctttgggtg tatttatcaa ccctactgtg
    39781 ccgctatcaa tatcttcctg tttgcaaccc aaaagtgaca gtaacgcagg ggcaattgtt
    39841 agaccagcaa ggccttttcc tgatatacgc aagaattcta atcgcgttaa gccaatatct
    39901 aatagtggac gatcaaccgg gttcatttat ttcgcatcct cttcgcaaac acagtgctga
    39961 caattcagca gcctgatatt aagcattcaa ctaatacgct gctgactctg tccccaacac
    40021 cagatatttt ccctaacccg atagtgtatg agaaaagatt taacgccgat aaaccaatcc
    40081 tttatggtta ggagaaa
//
LOCUS       DAZRUF010000011        39668 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZRUF010000011 DAZRUF010000000
VERSION     DAZRUF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.011"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(93..768)
                     /locus_tag="ACHXOL_000471"
                     /pseudo
     CDS             complement(93..768)
                     /locus_tag="ACHXOL_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(913..1521)
                     /locus_tag="ACHXOL_000472"
     CDS             complement(913..1521)
                     /locus_tag="ACHXOL_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR6807543.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1705..2022)
                     /gene="metJ"
                     /locus_tag="ACHXOL_000473"
     CDS             complement(1705..2022)
                     /gene="metJ"
                     /locus_tag="ACHXOL_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR6807544.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2299..3459
                     /gene="metB"
                     /locus_tag="ACHXOL_000474"
     CDS             2299..3459
                     /gene="metB"
                     /locus_tag="ACHXOL_000474"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR6807545.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAVGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3462..5894
                     /gene="metL"
                     /locus_tag="ACHXOL_000475"
     CDS             3462..5894
                     /gene="metL"
                     /locus_tag="ACHXOL_000475"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR6807546.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6033..6167)
                     /locus_tag="ACHXOL_000476"
     CDS             complement(6033..6167)
                     /locus_tag="ACHXOL_000476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6807547.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6243..7133
                     /gene="metF"
                     /locus_tag="ACHXOL_000477"
     CDS             6243..7133
                     /gene="metF"
                     /locus_tag="ACHXOL_000477"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR6807548.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7462..9642
                     /gene="katG"
                     /locus_tag="ACHXOL_000478"
     CDS             7462..9642
                     /gene="katG"
                     /locus_tag="ACHXOL_000478"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR6807549.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9736..10641
                     /gene="yijE"
                     /locus_tag="ACHXOL_000479"
     CDS             9736..10641
                     /gene="yijE"
                     /locus_tag="ACHXOL_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR6807550.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10668..11285)
                     /locus_tag="ACHXOL_000480"
     CDS             complement(10668..11285)
                     /locus_tag="ACHXOL_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR6807551.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11559..12662)
                     /gene="gldA"
                     /locus_tag="ACHXOL_000481"
     CDS             complement(11559..12662)
                     /gene="gldA"
                     /locus_tag="ACHXOL_000481"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR6807552.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12673..13335)
                     /gene="fsa"
                     /locus_tag="ACHXOL_000482"
     CDS             complement(12673..13335)
                     /gene="fsa"
                     /locus_tag="ACHXOL_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR6807553.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13347..15848)
                     /gene="ptsP"
                     /locus_tag="ACHXOL_000483"
     CDS             complement(13347..15848)
                     /gene="ptsP"
                     /locus_tag="ACHXOL_000483"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR6807554.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16157..17236
                     /locus_tag="ACHXOL_000484"
     CDS             16157..17236
                     /locus_tag="ACHXOL_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR6807555.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17251..17571
                     /locus_tag="ACHXOL_000485"
                     /pseudo
     CDS             17251..17571
                     /locus_tag="ACHXOL_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            17622..19918
                     /locus_tag="ACHXOL_000486"
                     /pseudo
     CDS             17622..19918
                     /locus_tag="ACHXOL_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            19884..20762
                     /locus_tag="ACHXOL_000487"
     CDS             19884..20762
                     /locus_tag="ACHXOL_000487"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR6807556.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20764..21105
                     /locus_tag="ACHXOL_000488"
     CDS             20764..21105
                     /locus_tag="ACHXOL_000488"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR6807557.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21092..21943)
                     /locus_tag="ACHXOL_000489"
     CDS             complement(21092..21943)
                     /locus_tag="ACHXOL_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR6807558.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22158..23891)
                     /locus_tag="ACHXOL_000490"
     CDS             complement(22158..23891)
                     /locus_tag="ACHXOL_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR6807559.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24074..26725)
                     /gene="ppc"
                     /locus_tag="ACHXOL_000491"
     CDS             complement(24074..26725)
                     /gene="ppc"
                     /locus_tag="ACHXOL_000491"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR6807560.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27077..28228)
                     /gene="argE"
                     /locus_tag="ACHXOL_000492"
     CDS             complement(27077..28228)
                     /gene="argE"
                     /locus_tag="ACHXOL_000492"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR6807561.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            28382..29386
                     /gene="argC"
                     /locus_tag="ACHXOL_000493"
     CDS             28382..29386
                     /gene="argC"
                     /locus_tag="ACHXOL_000493"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR6807562.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29394..30170
                     /gene="argB"
                     /locus_tag="ACHXOL_000494"
     CDS             29394..30170
                     /gene="argB"
                     /locus_tag="ACHXOL_000494"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR6807563.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30231..31604
                     /gene="argH"
                     /locus_tag="ACHXOL_000495"
     CDS             30231..31604
                     /gene="argH"
                     /locus_tag="ACHXOL_000495"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR6807564.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31871..32788
                     /gene="oxyR"
                     /locus_tag="ACHXOL_000496"
     CDS             31871..32788
                     /gene="oxyR"
                     /locus_tag="ACHXOL_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR6807565.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(32771..34171)
                     /gene="sthA"
                     /locus_tag="ACHXOL_000497"
     CDS             complement(32771..34171)
                     /gene="sthA"
                     /locus_tag="ACHXOL_000497"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR6807566.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34492..35142
                     /gene="fabR"
                     /locus_tag="ACHXOL_000498"
     CDS             34492..35142
                     /gene="fabR"
                     /locus_tag="ACHXOL_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR6807567.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35142..35501
                     /locus_tag="ACHXOL_000499"
     CDS             35142..35501
                     /locus_tag="ACHXOL_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR6807568.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35541..36641)
                     /gene="trmA"
                     /locus_tag="ACHXOL_000500"
     CDS             complement(35541..36641)
                     /gene="trmA"
                     /locus_tag="ACHXOL_000500"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR6807569.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36755..36945
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37010..38854
                     /gene="btuB"
                     /locus_tag="ACHXOL_000501"
     CDS             37010..38854
                     /gene="btuB"
                     /locus_tag="ACHXOL_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR6807570.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38799..39656
                     /gene="murI"
                     /locus_tag="ACHXOL_000502"
     CDS             38799..39656
                     /gene="murI"
                     /locus_tag="ACHXOL_000502"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR6807571.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9028 a         9981 c        10954 g         9705 t
ORIGIN      
        1 aggtaaaaat ctttggctgt gtattcagtc tggtaatcat ccaaggaaag cttatcagaa
       61 gggtgatgac tccaattagt agaacatata ttttcgataa aagcaccgat gactttttca
      121 tgaagttcta cagaacgcga gaaacagatg gttctgcggg ccagacgttt gattcgggtt
      181 ctcagggtca gattgttacg ctcaatgtgc tgagtaaaaa tcttaccagt cagatgcttc
      241 tccttcggta actctctagc gtaactaccc cattcgtcag tggtcaacat gccgatggta
      301 aaagggtaag cagcgcccgc aattcacgac aggtttcgtc agtccggggg ccaaaagtgt
      361 aggccagaac accaccagtt ttcgtgttgt acacgtacca gagccagtgc tgtcgcgctt
      421 tactgccgac aaagctccat tgctgactaa gtttgcagat gagtgccaca tcagcatgag
      481 cgaccgggga tgacgttatt cttcgtggcg agagtttttt aaagttcgga tgactgtatt
      541 aatgccgatt ttcagcgtac gggcagtatc gcgaaccccg gcaccgttga aagtcatttc
      601 agcgatttgg tctttaactc ccggtttacg gggctcgtaa cagtaactga gctgaaatac
      661 ccggtgacag tcacggccgc ggaatcgctc atgtcttttt gggttctgcc cgtgacggta
      721 aacctgaacg gactggcaac gggggcaatg aaggttaact gtgaccatga aacaacctta
      781 aaagcgagta ttatatcctt aattcaacta attggaggca tcaccgttta ccggagcgac
      841 agcttgcgga aaggtcgtgc aactacaaag caacaaacag actacggcgg gaattatttt
      901 tttcatcatc cattaccacg aaaacagcgg ccactctttg gcgactgtgg tgagttgtgg
      961 cgcgtcgaaa acggcttgtg gtgaaactac cgtttcctca agcggaatat ttttgccgta
     1021 gcgttctttg agcttagcct ggaccagcgg gttgctgaga tcgaactctt tcaacttctg
     1081 ctgctcgccc acgcgcattc ccagcgcgtt ctggtaaacc ttccacacca cttccgaaca
     1141 gtactggcga tcgtcgctcc atgagaagct gaaatcatac ggtttaccga gataacgttt
     1201 tgccgtttgc gccagttttt gctgttgttc aacactcagt ccgccttcaa cgcggcgaac
     1261 aacgtatttg cccttttcac catgggcgat ccactgcttg agcggggtgt atttcaccgg
     1321 gccgaccgct tcaaaaacgt agggcttttt gttgcgtatc accagcatac cggtgtggct
     1381 ataatcggaa tgggtcgcca gttggatcgc tttactttgc gatgagcgag agatctgaaa
     1441 gatgatgtcg ccggtttgtg gctgccaggc aaaggcaggt acagaaacca gcaggctgag
     1501 gatcagcagc ctgtttttca tagttaaacg tccatgtata aaaagcggtg ggtcgcagac
     1561 aacgtgctcg ttgtttatgc cggatgcggc gtgaacgcct tatccggcct acaagttcgt
     1621 gcaaattcaa taaattgcaa tatgacgtag gcctgataag cgtagcgcat caggcgattc
     1681 cactccgcgc cgctcttttt tgctttagta ttcccacgtc tccgggttaa tccccatctc
     1741 acgcatgatc tcttttgccg cttccgggat ttcgtcgctg cgctctttac gcagatcggc
     1801 atcatccggc aaaggttgcc cggtaaaggc atgcagaaac gcttcgcaca gcagctcgct
     1861 gttggtagcg tgacgcaggt tgttcacctg acgacgcgtg cgttcatcgg tgaggatttt
     1921 taacacctta agaggaatgg aaaccgtaat ctttttgact tgttcactct tcttgccgtg
     1981 ctcagcgtat gggctgatat attcgccgct ccattcagcc atgagatact taatcctctt
     2041 cgtcaataaa ttgagaccag accacacaat ttatgtgggt actgaccgta aacccgcata
     2101 gtttaccgta caggcgttac cgtgacatcg tgtaatgcac ctgtcggcgt gataatgcat
     2161 ataattttaa cggctatttg ggatttgctc aatctatacg caaagaagtt tagatgtcca
     2221 gatgtattga cgtccattaa cacaatgttt actctggtgc ctgacatttc accgacaaag
     2281 cccaggaaac ttcatcacat gacgcgtaaa caggccacca tcgcagtgcg tagcgggtta
     2341 aatgacgacg aacagtatgg ttgcgttgtc cctcccatcc atctttccag cacctataac
     2401 tttaccggat ttaatgaacc gcgcgcgcat gattactcgc gtcgcggcaa cccaacgcgc
     2461 gatgtggttc agcgtgcgct ggcagaactg gaaggcggtg ctggcgcagt acttactaat
     2521 accggcatgt ccgcgattca cctggtaacg accgtctttt tgaaacctgg cgatctgctg
     2581 gttgcgccgc acgactgcta cggcggtagc tatcgcctgt tcgacagtct ggcgaaacgc
     2641 ggttgctatc gcgtgttgtt tgttgatcaa ggcgatgaac aggcattacg ggcagcgctg
     2701 gcagaaaaac ccaaactggt actggtagaa agcccaagta atccattgtt acgcgtcgtg
     2761 gatattgcga aaatctgcca tctggcaagg gaagtcgggg cggtgagcgt ggtggataac
     2821 accttcttaa gcccggcatt acaaaatccg ctggcattag gtgccgatct ggtgttgcat
     2881 tcatgcacga aatatctgaa cggtcactct gacgtggtgg ctggcgtggt gattgctaaa
     2941 gacccggacg ttgtcactga acttgcctgg tgggcaaaca atattggcgt gacgggcggc
     3001 gcgtttgaca gctatctgct gctacgcggg ttgcgaacgc tggtgccgcg tatggagctg
     3061 gcgcagcgca acgcgcaggc gattgtgaaa tatctgcaaa cccagccgtt ggtgaaaaaa
     3121 ctgtatcacc cgtcgctgcc ggaaaatcag gggcatgaaa ttgccgcgcg tcagcaaaaa
     3181 ggctttggcg caatgttgag ttttgaactg gatggcgatg agcagacgct gcgtcgtttc
     3241 ctgagcgggc tgtcgttgtt tacgctggcg gaatcattag ggggagtgga aagtttaatc
     3301 tctcacgccg caaccatgac acatgcaggc atggcaccag aagcgcgtgc tgccgtcggg
     3361 atctccgaga cgctgctgcg tatctccacc ggtattgaag atggcgaaga tttaattgcc
     3421 gacctggaaa atggcttccg ggctgcaaac aaggggtaaa aatgagtgtg attgcgcagg
     3481 caggggcgaa aggtcgtcag ctgcataaat ttggtggcag tagtctggct gatgtgaagt
     3541 gttatttgcg tgtcgcgggc attatggcgg agtactctca gcctgacgat atgatggtgg
     3601 tttccgccgc cggtagcacc actaaccagt tgattaactg gttgaaacta agccagaccg
     3661 atcgtctctc tgcgcatcag gttcaacaaa cgctgcgtcg ctatcagtgc gatctgatta
     3721 gcggtctgct acccgctgaa gaagccgata gcctcattag cgcttttgtc agcgatctgg
     3781 aacgcctggc ggcgctgctc gacagcggta ttaacgacgc agtgtatgcg gaagtggtgg
     3841 gccacgggga agtatggtcg gcacgcctga tgtctgcggt acttaatcaa caagggctgc
     3901 cagcggcctg gcttgatgcc cgcgagtttt tacgcgctga acgcgccgca caaccgcagg
     3961 ttgatgaagg gctttcttac ccgttgctgc aacagctact ggtgcaacat ccgggcaaac
     4021 gtctggtggt gaccgggttt atcagccgta acaacgccgg tgaaacggtg ctgctggggc
     4081 gtaacggttc cgactattcc gcgacacaaa tcggtgcgct ggcgggtgtt tctcgtgtaa
     4141 ccatctggag cgacgtcgcc ggggtataca gtgccgaccc gcgtaaagtg aaagatgcct
     4201 gtctgctgcc gttgctgcgt ctggatgaag ccagcgaact ggctcgtctg gcggctcccg
     4261 ttcttcacgc ccgtacttta cagccggttt ctggcagcga aatcgacctg caactgcgct
     4321 gtagctacac gccggatcaa ggttccacgc gcattgaacg cgtgctggcc tccggtactg
     4381 gtgcgcgtat tgtcaccagc cacgatgatg tctgtttgat tgagtttcag gtgcccgcca
     4441 gtcaggattt caaactggcg cataaagaga tcgaccaaat cctgaaacgc gcgcaggtac
     4501 gcccgctggc ggttggcgta cataacgatc gccagttgct gcaattttgc tacacctcag
     4561 aagtggccga cagtgcgctg aaaatcctcg acgaagcggg attacctggc gaactgcgcc
     4621 tgcgtcaggg gctggcgctg gtggcgatgg tcggtgcagg cgtcacccgt aacccgctgc
     4681 attgccaccg cttctggcag caactgaaag gccagccggt cgaatttacc tggcagtccg
     4741 atgacggcat cagcctggtg gcagtactgc gcaccggccc gaccgaaagc ctgattcagg
     4801 ggctgcatca gtccgtcttc cgcgcagaaa aacgcatcgg cctggtattg ttcggtaagg
     4861 gcaatatcgg ttcccgttgg ctggaactgt tcgcccgtga gcagagcacg ctttcggcac
     4921 gtaccgactt tgaatttgtg ctggcaggcg tggtggacag ccgccgcagt ctgttgagct
     4981 atgacgggct ggacgccagc cgcgcgttag ccttcttcaa cgatgaagcg gttgagcagg
     5041 atgaagagtc gttgttcctg tggatgcgcg cccatccgta tgatgattta gtggtgctgg
     5101 acgttaccgc cagccagcag cttgctgatc agtatcttga tttcgccagc cacggtttcc
     5161 acgtcatcag cgccaacaaa ctggcgggag ccagcgacag caataaatat cgccagatcc
     5221 acgacgcctt cgaaaaaacc gggcgtcact ggctgtacaa tgccaccgtc ggtgcgggct
     5281 taccgatcaa ccacaccgtg cgcgatctga tcgacagcgg cgatactatt ttgtcgatca
     5341 gcgggatctt ctccggcacg ctctcctggt tgttcctgca attcgacggt agcgtgccgt
     5401 ttaccgagct ggtggatcag gcgtggcagc agggcttaac cgaacctgac ccgcgtgacg
     5461 atctctctgg caaagacgtg atgcgcaagc tggtgattct ggcgcgtgaa gcaggttaca
     5521 acatcgaacc ggatcaggta cgcgtggagt cgctggtgcc tgcccattgc gaaggcggca
     5581 gcatcgacca tttctttgaa aatggcgatg aacttaacga gcagatggtg caacggctgg
     5641 aagcggcccg cgaaatgggg ctggtgctgc gctacgtggc gcgtttcgat gccaatggca
     5701 aagcgcgtgt gggcgtggaa gcggtgcgtg aagaccatcc gctggcatca ctgctgccgt
     5761 gcgataacgt ctttgccatc gaaagccgct ggtatcgcga taatccgctg gtgatccgtg
     5821 ggcctggcgc tgggcgcgac gtcaccgccg gggcgattca gtcggatatc aaccggctgg
     5881 cacagttgtt gtaatttcag aaatttaata atgcccggta ctcatgtttt cgggtttatg
     5941 gtttctaatg aaatatattg aattatcata ggattaggcc ggattaagcg tttacgacga
     6001 atccggcaag aagcaataag tacatggtta gtttatattt gcagtccggt ttgctttgca
     6061 taccggattt tctttttctt accatcctga agttttttca tcttccctga tttttcctca
     6121 ccatcattgg tcatttttcg gttgacgccc ttcggctttt ccttcatctt tacatctgga
     6181 cgtctaaacg gatagatgtg cacaacacaa catataacta caagcgattg atgaggtaag
     6241 gtatgagctt ttttcacgcc agccagcggg atgccctgaa tcagagcctg gcagaagtcc
     6301 aggggcagat taacgtttcg ttcgagtttt tcccgccgcg taccagtgaa atggagcaga
     6361 ccctgtggaa ctccatcgat cgccttagca gcctgaaacc gaagtttgta tcggtgacct
     6421 atggcgcgaa ctccggcgag cgcgaccgta cgcacagcat tattaaaggc attaaagatc
     6481 gcactggtct ggaagcggca ccgcatctta cttgcattga tgcgacgccc gacgagttgc
     6541 gcaccattgc acgcgactac tggaataacg gtattcgtca tatcgtggcg ctgcgtggcg
     6601 atctgccgcc gggaagtggt aagccagaaa tgtatgcttc tgacctggtg acgctattaa
     6661 aagaagtggc agatttcgat atctccgtgg cggcgtatcc ggaagttcac ccggaagcaa
     6721 aaagcgctca ggcggatttg cttaatctga aacgcaaagt cgatgccgga gccaaccgcg
     6781 cgattactca gttcttcttc gatgtcgaaa gctacctgcg ttttcgtgac cgctgtgtat
     6841 cggcgggcat tgatgtggaa attattccgg gaattttgcc ggtatctaac tttaaacagg
     6901 cgaagaaatt tgccgatatg accaacgtgc gtattccggc gtggatggcg caaatgttcg
     6961 acggcctgga tgatgatgcc gaaacccgca aactggtggg cgcgaatatt gccatggata
     7021 tggtgaagat tttaagccgt gaaggagtga aagatttcca cttctatacg cttaaccgcg
     7081 ctgaaatgag ttacgcgatt tgccatacgc tgggtgttcg acctggttta taaatagtgt
     7141 ggcttttgtg aaaatcacac agtgatcaca aattttaaac agagcacaaa atgctgcctc
     7201 gaaatgaggg cgggaaaata aggttgtcag ccttgttttc tccctcatta cttgaaggat
     7261 atgaagctaa aacccttttt tataaagcat ttgtccgaat tcggacataa tcaaaaaagc
     7321 ttaattacga tcaatttgac ctacatctct ttaaccaaca atatgtaaga tctcaactat
     7381 cgcatccgtg gattaattca attataactt ctctctaacg ctgtgtatcg taacggtaac
     7441 actgtagagg ggagcacatt gatgagcacg tcagacgata tccataacac gacagccact
     7501 ggcaaatgcc cgttccatca gggcggtcac gaccagagcg cgggggcggg cacaaccact
     7561 cgcgactggt ggccaaatca actccgtgtt gacctgttaa accaacattc taatcgttct
     7621 aacccgctgg gtgaggactt tgactaccgc aaagaattca gcaaattaga ttactacggc
     7681 ctgaaaaaag atctgaaagc cctgctgaca gaatctcagc cgtggtggcc agccgactgg
     7741 ggcagctacg ccggtctgtt tattcgtatg gcctggcacg gcgcaggtac ttaccgttca
     7801 atcgatggac gcggcggcgc gggtcgtggt cagcaacgtt ttgcaccgct gaactcctgg
     7861 ccggataacg taagcctcga taaagcgcgt cgcctgttgt ggccaatcaa acagaaatat
     7921 ggtcagaaaa tctcctgggc cgacctgttt atcctcgcag gtaacgtggc gctggaaaac
     7981 tccggcttcc gtaccttcgg ttttggtgcc ggtcgtgaag acgtctggga accggatctg
     8041 gatgttaact ggggtgatga aaaagcctgg ctgactcacc gtcatccgga agcgctggcg
     8101 aaagcaccgc tgggtgcaac cgagatgggc ctgatttacg ttaacccgga aggcccggat
     8161 cacagcggcg aaccgctttc tgcggcagca gctatccgcg cgaccttcgg caacatgggc
     8221 atgaacgacg aagaaaccgt ggcgctgatt gcgggtggtc atacgctggg taaaacccac
     8281 ggtgccggtc cgacatcaaa cgtaggtcct gatccagaag ctgcaccgat tgaagaacaa
     8341 ggtttaggct gggcgagcac ttacggcagc ggcgttggcg cagatgccat tacctctggt
     8401 ctggaagttg tctggaccca gacgccgacc cagtggagca actatttctt cgaaaacctg
     8461 ttcaaatatg agtgggtaca gacccgcagt ccggctggcg caatccagtt cgaagcggta
     8521 gacgcaccgg aaattatccc ggatccattc gatccgtcga agaaacgtaa accgacgatg
     8581 ctggtgaccg acctgacgct gcgttttgat cctgagttcg agaagatctc tcgtcgtttc
     8641 ctcaacgatc cgcaggcgtt caacgaagcc tttgcccgtg cctggttcaa actgacgcac
     8701 agggatatgg ggccgaaatc tcgctacatc gggccggaag tgccgaaaga agatctgatc
     8761 tggcaagatc cgctgccgca gccgatctac aacccgaccg agcaggacat tatcgatctg
     8821 aaattcgcga ttgcggattc tggtctgtct gttagtgagc gggtatcggt ggcctgggca
     8881 tctgcttcta ccttccgtgg tggcgacaaa cgcggtggtg ccaacggtgc gcgtctggca
     8941 ttaatgccgc agcgcgactg ggatgtgaac gccgcagccg ttcgtgctct gcctgttctg
     9001 gagaaaatcc agaaagagtc tggtaaagcc tcgctggcgg atatcatcgt gctggctggt
     9061 gtggttggtg ttgagaaagc cgcaagcgcc gcaggtttga gcattcatgt accgtttgcg
     9121 ccgggccgcg ttgatgcgcg tcaggatcag actgacattg agatgttcga gctgctggag
     9181 ccaattgctg acggtttccg taactatcgc gctcgtctgg atgtttccac caccgagtca
     9241 ctgctgatcg acaaagccca gcaactgacc ctgaccgcgc cggaaatgac cgcgctggtg
     9301 ggcgggatgc gcgtactggg tgccaacttc gatggcagca aaaacggcgt cttcactgac
     9361 cgcgttggcg tattgagcaa tgacttcttc gtgaacttgc tggatatgcg ttacgagtgg
     9421 aaagcgaccg acgaatcgaa agagctgttt gaaggccgtg accgcgaaac cggcgaagtg
     9481 aaatacactg ccagccgtgc ggatctggta tttggttcta actctgtcct gcgtgcggtg
     9541 gctgaagttt acgccagcag cgatgctcac gagaagtttg ttaaagactt cgtggcggca
     9601 tgggtgaaag tgatgaacct cgaccgtttc gacctgctgt aatctgacct tcttcagcga
     9661 ctgcctttca ggcagtcgct gaagtttctt taccggcgta tagtgtccac aggaaaacta
     9721 cacactggat ctctcatgtc tgccgcagga aagagcaacc cactggcaat cagtggcctg
     9781 gttgtgctca cacttatctg gagttatagc tggattttca tgaagcaagt caccagctac
     9841 atcggtgcct tcgactttac cgccttacgc tgcattttcg gcgctctcgt tttattcatc
     9901 gtccttttat tacgtggtcg cggaatgcgc ccgacaccgt ttaaatacac cttagccatt
     9961 gccctgttac aaacctgcgg gatggttggc ctggcgcagt gggcgctggt cagcggcgga
    10021 gcggggaagg tggcgatcct aagctatacc atgccgttct gggtggtgat tttcgccgcg
    10081 ttgtttctcg gtgaacgcct gcgacgtggg caatatttcg cgatactgat tgccgctttc
    10141 ggcttatttt tggtgttgca gccgtggcaa ctcgatttct cttcgatgaa aagtgccatg
    10201 ctggcaatcc tctccggcgt cagttggggg gcgagcgcga ttgttgctaa acgtctgtat
    10261 gcccgtcatc cgcgcgtgga tttattgtcg ttaacatcct ggcagatgct ctatgcggcg
    10321 ctggtgatga gtgtggtcgc tttactggtg ccgcaacgtg aaattgactg gcagcccacc
    10381 gtgttctggg cgctggccta cagtgcgatt ctggcgacgg cactggcgtg gagcttatgg
    10441 ttgtttgtat tgaaaaactt gcctgccagt attgccagct taagcacact ggccgttccc
    10501 gtttgcggcg tactcttttc ctggtggctg ctcggcgaga atccgggggc cgttgaaggt
    10561 agcggtattg tgctgattgt gatggcactg gcgctggtga gccgtaagaa aaaagaagcc
    10621 gttagtgtaa aaaggatctg aattttttct tcatgtgggg cgatcgctta tttaacaaaa
    10681 taacgataat gccccaccat gcgccagcta aacagcacat cttcttcctg cgcacctgcg
    10741 ccaatgttat gtatcaccag cggcgtaccg tcgcgggcga agccatctga aaccacccca
    10801 atatgtgcca gcccgttatc taatcgccag gagacaatat cgcccgcttg atagtcactg
    10861 gggttcttgc tgatggggcg tgttttatcg tggtgggtaa accaggtttc cagattaggc
    10921 acccgacggt gatcgatgtt gctgtccggg cgctttaact gccatttttg cgggtactcg
    10981 gcaaaattct tcgccatatc ttcgtgcacc agtttctgca aatcgacctt ctggctgcgc
    11041 aatgcacgga tcaccacatc ggaacatacc ccgcgttttt gcgggacatc accgccagga
    11101 taggtaagct gcatatacgc cggatcgtaa aatagcgtgc tgccgatttg ctgtctggca
    11161 ccgtctgcga tggcaaggtt ggtattggcc tggatttgta ccacggttgg tggaacggcg
    11221 ggagatttta aggagtggct ggtaaatgcc gttagcaggc tgagcagcgc cagtgaagct
    11281 ttcatctggt tcgtccgtga aaataagagt cacagattcg accttccggg ataacgattg
    11341 ttctgtagag aaaatgtcca gaagcggata aaaaactcct gaacaacgtg ggagtttcag
    11401 attactggca cagagtgccg gatgcggcgt gaacgcctta tctgtcctaa aaaacaagaa
    11461 aattcaatat attgcagaga ttgcgtaggc ctgataagcg tagcgcatca ggcaattttg
    11521 cgttcaaact cccggacaag ccgggagttt ggagtaggtt attcccactc ttgcaggaaa
    11581 cgctgaccgt actggtcggc taccagcaga gcggcgtaaa cctgatctgg cgtcgcgccg
    11641 ccaggcatgt tgtggatggt ttcacattct gcacatgccg cttcagccac aattcgcatt
    11701 ttcgccggga catcttcttt aatatccagt tgagcgagag ttattggcaa acctaccgca
    11761 tggctaagtg cagctacggt ttcgatttcc tcaaccggtg cgttttccag aaccagctgc
    11821 gtcagcgtac caaatgccac tttttcaccg tgataatagt gatgcgcgtc cgggatagcg
    11881 gtcaggccgt tatgcactgc gtgcgccgca gccagaccac cactttcaaa accaacaccg
    11941 ctcaaatagg tgttcgcttc aatcacgcgc tccagcgccg gagtcactac atgctgttcg
    12001 gcagcaagca tcgctttttc gccttcttcc agcagggtgt tgtagcacag ttcagccagt
    12061 gccagcgcag cctgggtgca cttgccgccc gccatggtgg tcgcgccgct acgagagcag
    12121 gcacgcgctt caaaccaggt tgccagcgca tcgccgatac ccgccgctaa cagacgtgca
    12181 ggtgcgccag cgacgatttt ggtgtcgaca atgaccatat tcgggttatt tggcaacagc
    12241 agatagcggt caaactcacc ctcatcggtg tagataacag acaatgcgct gcacggtgca
    12301 tcggtagagg cgatagtcgg tgcgatcgct accggaacac ccatgaaatg tgccagtgct
    12361 ttggctgtat cgagggtttt tccgccaccg ataccgagaa ttgcgccaca ctgcgcagtc
    12421 tccgcgatgc cacgcagacg gtcgatctca ttttgcgaac atccaccgcc aaacggcgca
    12481 atttctacta ccagtccagc atctttaaag cttttctcga cagtggattg agcaaaacct
    12541 aaaacaaatt tgtcacccac cactaaccag cgttctgcca gcggcttcag gtattcgccc
    12601 agacgattaa tcacatcagc gccctggatg tatttacccg gtgattgaat aatgcggtcc
    12661 ataattgctc ctttagagat gagtagtgcc aaatgcggca ttccagtcgt gttcgaactt
    12721 ctctatagct gactctaccg caggggtgtt gagcatttgt tgcgctacat ctaagggcag
    12781 ggtgatggat tcacatcctg ccagtaaaca gtccagcgcc tgacgcggcg ttttaaagct
    12841 ggctgccagc accatgcttt ctggcgcgtg catttctaac agcgcttgca gctcctgaac
    12901 cgtacgaatg ccgtctccgc cctgggcatc tacgcggtta acatacggag caacgtattt
    12961 tgcccctgcc agcgcggcca gtaacccttg tgcagcactg tacactgcgg tgccaagcgt
    13021 agtaataccc tcttttttca gtattttaat tgctgccaga ccttcggaag tcaccgggat
    13081 tttcaccaca atacccggaa tagcgtcgcg caggcgcttc gcttcttcca ccatcccctg
    13141 cgcgtcgcgg ctcatggtct gagcaaacag aatgccctca tcaccaatcg ctttttgcag
    13201 acgcggcagc acttcccata tggactcctt gctggcagcg ataatgctcg ggttagttgt
    13261 caccccggca atggggaata tgcgtgccag acgttcgact tctgcgacgt tagcggtgtc
    13321 cagatacagt tccatgatgt ttccttttac agttccagtt catgttgcag caggctggcg
    13381 atagcgtcgg cagaggcggc gttaaccagc gcgttacgga attcttcgtg catgatgcgg
    13441 cgagcgaggc gcgagaaaat gcgcatgtgc tgatcgcccg cagcgtgttt gttcagggtt
    13501 aacatgatga tgaattgcgc ttcatcatca ccccagcgca ccggcgcttg cagacgcgca
    13561 acgctgatgg tggattgctc aatgtgttct gatttgctgt gtggaatggc aaaactaaag
    13621 cccagaccgg tagagaaaac ggcctcgcgc gcccacaagt cggcttccag tttacgcgga
    13681 tagcggcagc gccctgccag cagcaggtta tcggtcatgc ctttgagcac ttcttctttg
    13741 ctgcgccagt cgctttccag tgtgatgcac tcggcggtga ccagcggtgc gtcttgttgg
    13801 gtcatgcgga attgcgccag caggtgctcc acttccagcg aagtacggca ggccattgcc
    13861 tggttgagca acttgcggca ctcacggcta tcaagttgcg ccatccgcgc tttcgccgcc
    13921 ggaattgatg gtgcgctcat gctgagttca tccagcccta agccgaccag caacggcagc
    13981 acggaacctt tcgctcccag ctcaccgcac agaccaatcc atttaccctg gcgatgcacc
    14041 gcctgcacgg cgtaatcgag cgcccgcaag aatgccggat tcaggctgtt gtagtgacga
    14101 gtaaccttag cgttatcgcg atccactgcc agcagatatt gcgtcaggtc attactacca
    14161 atactaaaga aatcaatctc ttcgcagcat tgatcgatga tgaacatcac cgacggcact
    14221 tccagcataa tgccgagctg gatcttctca tcaaacggaa tgtgttcgtt acgtagttgc
    14281 tgtttggctt ctgccagttt ttctttcacc cataagatct cttccattga ggagatcatc
    14341 gggatcatga ttttcaggct gccgtgagcg gaggcgcgca ggatcgagcg tagttgtgtg
    14401 gtaaataacg acgcgtattc ttcataaata cgcacggcgc gataaccgag gaacgggttt
    14461 gcctctgcgg gaatgtttag ataatcaacg ggtttgtcgc cgccaatgtc catagtgcgc
    14521 acaataatgc tgcgtccgtt ggcagactcc agcgcctgac aaaaaatgtt gtacagctcg
    14581 ctttcgcccg gtgcactggt gcgatccata tagagcattt cagtgcggaa caaaccaacg
    14641 ccttccgcac cattgccgaa tgcggcctgc gcttccacgg agtgagcgat gttagcggca
    14701 atttcaatgc ggataccgtc agcggtacgg gcttgttggg tcagccagac acgctgttgc
    14761 tcacgcaggg cgtcctgtac gcgggcttct tgctgataat aacgagctac ggcttctcct
    14821 ggctcaacca caatcgcccc ggcgttgccg tcgatataaa tcgtttgatg ctgccacgga
    14881 gtgagggcat caatatccac accaaccagc gtcggaatgt tgaacgaacg ggcaaggatc
    14941 accgtatgtg aggtggtgcc gccacttttc agcaacagac ctttgaggtg atttttatcc
    15001 agttcgagga actggctggg ggtcagttca tcagccatac aaatggcgga ctgcgtcagt
    15061 ttgcccggtg ccgggaagcg ttgctcaccg tagatttgct ggagtaactg gaagcagacg
    15121 tcgcgtacgt ccagggcacg ttcttgcagg tagctgctgc tggaacgggc gaactcttcg
    15181 caaaagtgat tcgcactggc gacaatcgct tcggcgcagc ttaatccctc gctgacgcct
    15241 gccagtaagt gctgacgtaa agatgtatcg ccagccaggg atcggtgagc ttccagtatc
    15301 gcgctggtag caccgtcgct atccagcaga cgaaactcaa tgtttttcag taccagcgtc
    15361 aggccgtttt ccagtgtgga ttgctcggcg tcaacgtctt tggctgcggg aagattactc
    15421 agcgcattga gatctaaaga agagatcggc gtcagaatgc cgcctgcact accgctgcac
    15481 acggtgcggg cgcggataat ttgcggattc agattggtca gtgaaaccgg cagtggttcc
    15541 agttcgtcag atttaacttc cgccagcggc gcatcgcagt gggggaattc atcgcgcagc
    15601 cattggctta aacgctggtg cgcttcctgt tcgtcggccc cggaaatcag taactggcag
    15661 ttatcgcccg ccagcgtatc ggtgccaatc agcgcaaggg cgcttttggc gttgcccttg
    15721 cggtcagtgt gcaggttatg ccactcaatt tgtgatgaaa aagtattaca cagcgtttca
    15781 acgtggcttg ccggacgcgc atgtacgccg ttaggtagct cacaaataaa ttccacaatc
    15841 agggccattg cctctctccc ggtacggttt tctgtttaca ggataagagc gcacggcaac
    15901 ggcctgccat gtgacaaatc tgccaaaagc tggacaaatg taatgtaacc gtcaatttgc
    15961 gacgcgtctc acaagacgcc gttttgcggc atgcttccgg tttatcgcaa gttatgaggc
    16021 ggatcgcatt tttgtactga tattacaaaa gtccagtaaa tggccttttt atccactgtt
    16081 tgctccgcct gcgattgcct attgtttcct ccatcaaaaa tatggacatg ggctttcgca
    16141 agcccgggag cagcttatga atgagttggt gcagatcctg aaaaataccc gtcagcattt
    16201 aatgacgggc gtttcacaca tgattccctt cgtggtatcg ggcggtattt tgctggcggt
    16261 ttccgtcatg ttgtatggca aaggcgcagt gccggatgcc gtagccgatc caaatctgaa
    16321 aaaactgttt gatatcggcg ttgcgggctt gacgctgatg gtgcctttcc tcgccgctta
    16381 cattggttat tccattgcag aacgttctgc gctggctcca tgcgctatcg gtacctgggt
    16441 tggtaacagc tttggtgcgg gcttctttgg tgcactgatc gccgggatta tcggcggcat
    16501 cgtggtgcat tacctgaaga aaattccggt gcataaagtt ctgcgctcgg tgatgcctat
    16561 cttcattatt cctatcgtcg gcacactgat taccgcaggc atcatgatgt ggggcttggg
    16621 cgagcctgta ggggcgttga ccaacagcct gactcagtgg cttcagggga tgcagcaggg
    16681 cagcattgtt atgctggcgg tgatcatggg tctgatgctg gcgttcgata tgggcggtcc
    16741 ggttaacaaa gtggcctatg ccttcatgct gatttgcgtt gctcagggtg tttataccgt
    16801 ggtggccatc gctgccgtgg gtatttgtat cccgccgctg gggatggggc tggcgacgct
    16861 gattggtcgt aaaaatttct ccgcagaaga gcgcgaaacc ggcaaagcgg cactggtgat
    16921 gggctgtgtt ggcgttactg aaggggcgat tcctttcgcc gctgccgatc cgctgcgtgt
    16981 cattccttcc atcatggtcg gttcagtttg tggtgcagta actgcggcgc tggtcggtgc
    17041 gcagtgctat gcaggctggg gtggtctgat tgtgctgccg gtggttgaag gcaagctggg
    17101 ttatatcgca gcagtggctg tcggagcagt ggtgacggct gtttgtgtga acgtgctgaa
    17161 aagtctggcg cgtaaaaatg ggtcttcgac tgatgaaaaa gaagacgacc tggatttgga
    17221 ttttgaaatt aattaattga ggaacgagcc atgacgaaaa ttattgcagt aaccgcatgt
    17281 ccttcaggtg ttgcccatac ctatatggca gcagaggcgc tggaaagtgc cgcgaaagcg
    17341 aaaggctggg aagtgaaagt agaaacctag gggtcaattg gtctggaaaa tgaactgact
    17401 gcggaagatg tggcgagcgc tgatatggtg attctgacca aagatatcgg catcaagttt
    17461 gaagagcgtt ttgctggcaa gaccatcgtg cgcgtcaaca tcagcgatgc agtaaaacgt
    17521 gctgacgcca ttatgagcaa aattgaagct cacctggcgc aaaccgctta agttcttctc
    17581 ccgctcgcaa gggcgggttc gctttcccac aggagttcct catgacgaat cgtatctctc
    17641 gcctcaaaac tgcactgttt gccaataccc gtgaaatctc gctggagcgg gcgctgcttt
    17701 ataccgccag ccatcggcaa accgaaggcg aaccggtgat attgcgccgg gcgaaagcaa
    17761 cagcgtatat ccttgaacat gttgaaattt cgattcgtga tgaagaactg attgccggta
    17821 accgcaccgt aaaaccgcgc gccgggatta tgtcgccgga aatggaccct tactggctgc
    17881 tgaaagagct ggatcaattc ccgacgcgtc cgcaggaccg ctttgctatc agcgaagaag
    17941 ataaacgtat ctaccgtgaa gagttgttcc cgtactggga aaaacgttcg atgaaagatt
    18001 tcatcaacgg gcagatgaca gatgaagtaa aagccgcgac caacacgcag attttcagca
    18061 tcaaccagac ggataaaggc caggggcaca ttattattga ttacccacgc ctgttgaatc
    18121 acggtctggg tgagctggtg gcacagatgc agcaacattg tcagcaacag ccggagaatc
    18181 acttttatca ggccgcgttg ttactgctgg aagcctcgca gaaacacatt ttgcgttacg
    18241 ccgaactggc ggaaacgatg gcggcaaact gcacagatgc acagcgtcgc gaagaactgc
    18301 tgactattgc ggagatctct cgccataacg ccgaacataa gccgcagacg ttctggcagg
    18361 cgtgccagtt attctggtac atgaacatca ttctgcaata cgaatccaac gccagttcgc
    18421 tgtctttagg gcgcttcgac cagtatatgt tgccgttcta tcaggcatct ttaacccagg
    18481 gcgaagatcc ggcgttcctg aaagaactgc tcgaatcttt atgggtgaaa tgcaacgaca
    18541 tcgtgctgtt gcgctccacc agcagcgcgc gttatttcgc aggtttcccg accggctata
    18601 ccgcactgct cggcgggtta accgagaacg gacgtagcgc ggtgaacgtg ctttcgttcc
    18661 tttgccttga cgcctatcaa agcgtgcaat taccgcaacc gaacctcggc gtgcgcacta
    18721 acgccttgat cgacacgccg ttcctgatga aaaccgccga aaccattcgc ctcggcaccg
    18781 gtattccgca aatctttaac gatgaagtgg tggtaccagc gttcctcaac cgtggcgttt
    18841 cgctggaaga tgcgcgcgac tattccgtag tgggctgtgt ggaattatct attcccggca
    18901 gaacctacgg cttgcatgac atcgcgatgt ttaatctgct gaaagtgatg gaaatctgcc
    18961 tgcatgaaaa tgaaggcaat gctgcgctga cttatgaagg tttactggag cagatccgcg
    19021 ccaagatcag ccactacatc accctgatgg ttgagggcag caatatttgt gatatcggcc
    19081 atcgcgactg ggcacctgta ccgctgctct cgtcttttat cagcgattgt ctggaaaaag
    19141 gtcgcgatat taccgatggc ggcgcgcgtt ataacttctc cggcgtacag gggatcggta
    19201 tcgccaacct gagcgattct ctccatgcgt tgaaagggat ggtttttgag caacagcgtt
    19261 taagttttga cgaattgctg tcggtattaa aagccaactt tgcaacgcca gaaggcgaaa
    19321 aagtccgtgc tcgcttaatt aaccgcttcg agaaatacgg taacgatatc gacgaggtgg
    19381 ataacatcag cgccgaactg ttacgccact actgcaaaga agtggaaaaa taccagaacc
    19441 cgcgcgcggt tacttcacgc cgggatcgta taccgtttct gctcacgtcc cgttgggatc
    19501 ggtggttggc gcgacgccag acggtcgttt tgccggggaa caactggcag acggcggctt
    19561 gtcacctatg ctgggccagg acgcacaagg gccaacagcg gtactgaagt cagtcagtaa
    19621 gctcgataat acgctgctgt ctaacggtac gttgctgaac gtgaaattca ctccggcgac
    19681 cctggaaggt gaagcgggat tacgcaaact ggccgacttc ttacgggcgt ttacccagct
    19741 taagttgcag catattcagt ttaacgtggt gaacgccgac acgttgcggg aagcgcaaca
    19801 gcgcccacaa gattatgccg ggctggtggt gcgcgttgcc ggatacagcg ccttctttgt
    19861 cgaactgtcg aaggagatcc aggatgacat catccgccgg acagcgcatc agctgtaacg
    19921 ttgtggaaac gcgccgcgat gatgtggcgc gcattttcaa cattcagcgt tattcactga
    19981 atgacggcga gggcattcgt acggtggtct tttttaaagg ctgtccgcat ctttgcccgt
    20041 ggtgtgctaa tccggagtcg atctccggca aaatccagac ggtacgcaga gaggcgaaat
    20101 gtctgcactg tgcgaaatgt ttgcgtgatg cggatgaatg cccctccggg gcgtttgaac
    20161 ggattggtcg cgatatcagc cttgacgctc tggaacggga agtgatgaaa gatgacattt
    20221 tctttcgcac gtccggcggc ggcgtcacgc tttctggcgg cgaagtgtta atgcaggcgg
    20281 agtttgctac ccgtttttta cagcgactgc ggctgtgggg tgtgtcatgc gccattgaaa
    20341 ctgccggaga cgcgccagca agcaagctgt taccgctggc gaaattgtgc gatgaagtgc
    20401 tgttcgattt aaaaattatg gacgcgactc aggcgcggga tgtggtgaag atgaacctgc
    20461 cacgcgtgct ggagaatctg cgtttgctgg tgagtgaggg tgtcaacgtg atcccgcgtt
    20521 taccgttgat ccctggtttc acgctcagca gggagaatat gcagcaggcg ctggatgtac
    20581 tgatcccgct gaaaatcagg cagatccatc tgttaccgtt tcatcagtac ggcgaaccga
    20641 aataccgcct gctggggaaa acatggtcga tgaaagaggt gcctgcgccg tcgtcagccg
    20701 atgtgacaac gatgcgcgaa atggcagaac gggccggatt tcaggttacc gtgggaggtt
    20761 aaaatggcat acctggtggc agtaaccgcc tgcgtaagcg gcgtggcgca tacttatatg
    20821 gcggcggaac ggctggaaaa gttgtgccag ttagagaagt ggggcgtcag cattgaaact
    20881 cagggcgcgc tgggaacgga gaatcgttta gcggacgagg atatccgtcg ggcggatgtt
    20941 gctctgttga ttacggatat cgagcttgcc ggtgccgagc gatttgaaca ttgccgctat
    21001 gtgcaatgca gcatctacgc attcctgcgt gagccgcagc gggtaatgag cgcggtgcgc
    21061 aaagtgcttt ctgcgccgca gcaaacccat cttattctgg agtagtcggt ttttctgtca
    21121 gttggctgtg gtactgccgg cgatattccg acggcgagcg ttcggtgttt ttacgaaaca
    21181 gacggcagaa gtagttgctg tcgacaaagc cgcaggcgtg cgccacttct tttaccttca
    21241 ggtcatagcc tttcaataac gtcttagcgt gctccagtcg cgtgtgattc aggtattcgt
    21301 taaaaccaat ggcccccgtt ttttgaaaca ggtgcgagag atagtttggt gaaatataga
    21361 atgcctgtgc gacagattcg cgggtaagcg cggaggcata gcgttcgtcg atataatcgc
    21421 gaatagcttc aaatagtgcc tggctgcgtg aggcggtctg gatttggctg ccgagcaaat
    21481 cgcggcagtg gctaagcagg ctggctacga taagccttgc cgtttgctgc tcctgcggct
    21541 gcatttgcat ttcattgagc gtttgtagca gaaaagaacc tatacgtggg ccgcgtcggg
    21601 cgacgtgttg cttcgccaga ttttgatatt gtttgccatc ccattgcacg acgctgaagc
    21661 cgagttgctg tttgccaaac agcacgctaa aggtggtagc gggtgttttc cattgtggga
    21721 aattccagcc accagcggca acgtacagca catcgccggg aactaacgtt gctccagcgc
    21781 cggtatcaac aaactcacct tccagcacaa tttccagccg tggaaaatcg acctgatacg
    21841 caagatcggg caccgggccg ttactgctgg caaagtaaat ctggcgcagg gacagcgggc
    21901 cgttgatcag gcgggagagc agatagctga cgtcgtgata catgttattc ccttaggtat
    21961 gtagacggga aaacagtaac tggcattatt aaaaaaggcc acctgaaaag tgtggcctga
    22021 ggggagttcg atgtaaacat tgcggcagtt gtattgccgg atgtggcgta aacgccttat
    22081 ccagcctacc gccgcgatct gtaggccgga taagacgcgt caagcgtcgc atccggcaaa
    22141 tagcgcttgg ctgataatta ctgattacct acctggtcgc catacggcag agtgtcgtaa
    22201 tcgatcagcg cgtttttctt gtacgggtta ccaatccagc gggtagtttc tttgaactgc
    22261 ggattcacca ctgaacgggt tggatcgtaa ccgtcgtaag ataagcccgc caaatctgac
    22321 caggtgtgga tcagttccgc caggctgtat ttacgatcga catcctgtga gaaatcacgg
    22381 ggatgagtcg cttgccattt ttccgacgtc cacagcagga acggaatggt gtacatgtgg
    22441 cgcgtcgggt tgtcttcgtt acgcccctga gttttgtgcg gcggcgtgtc gtaaacctct
    22501 tcaccgtggt cagagaagta aaccaggaat ccgttcgggt ctgccgcttt aaagtcttta
    22561 atcaggctgg caaccacatg atcgttatac aggttagcgt tgtcataatc gttatatgat
    22621 tccagctctt ctgcgcttaa tcctggcgga acatgatcgg tattgccatc aaacttgccc
    22681 tgattttccg ggtagcggta tttgtatttg atatgcgtac ccagcagatg aacgatgatc
    22741 agtttcttcg gcgcagggtc attcagcaca tcctggaacg gcttcagcac gttggtgtcg
    22801 tattcacgcg cactctgcgt acgttgctgg ttcatgtagt actgcttgtc ggtctggcgc
    22861 gaaaataccg tcagcatggt attgcgggcg gtcatcgtct gctggttggt gatccagaag
    22921 gttttataac ccgcctgttt catcatgttc atcagcgacg gctgcgtcag atacagatcc
    22981 gggttctttt cattggcaaa ggtcagcgcc tgttgcagga tttcaatggt gtacggacga
    23041 gacgtaacta cgttattaaa cacggtcaga ttcggatcgg ttttatgcag cgcatccagc
    23101 tccggcgtgg tttcacgcgg ataaccgtac agactcatgc gtccgcgctg ggtcgactcg
    23161 ccaatcacca gcactaaagt gcgcggttcg ttacccgatt catctttgaa attagccagt
    23221 ggcggcaagg cattattttc attcagtaac tttgttagcg agtttagttg ctgacgatac
    23281 tgataatagc cggtcaggaa ttgccacggt gcggcaggct ccatacgcga ggccaggtta
    23341 tccaacgttt tctcaaacgg cttgtttttg ataaacgtat tcatggcgat cggatgcaga
    23401 atcaagccat aaagcagggc aaaagagaca acataacgcc acggctttgg aatatagacc
    23461 gggcgcaggc gtgtccacag cagaactgcc accgccgtat aggccagcgc gataagcaca
    23521 attttcaggc tgaaatactg gcttaaatac tcgctggctt cgttggtgtt ggtttcgaac
    23581 atcacaaaca gaacgctctg cgagaactcc tgcccgtaga tgacgtagta gcatagcgcc
    23641 gccagagagg ccgcccatag caccacgccg attactgcgg caataatttt aatccgcttc
    23701 ggaaagagga ataccgggat caaccacagc gagctgaata acagcgagtc gcgaatgccg
    23761 ttggtgccac tataaccact gatgtaaata atggcctgta gcagagtaga gaaaaaccaa
    23821 aagtagagca gtgcccaacc cagggctttc cagctaaaaa ggggtttagc ctggacttct
    23881 gtggaatgca tagtaagaac ctgtcttgaa aaaaatatcg ccgaatgtaa cgacaattcc
    23941 ttaaggatat ctgaaggtat attcagaatt tgaataaaat gcagacagaa atatattgaa
    24001 aacgagggtg ttagaacaga agtatttcag aaaaccctcg cgcaaaagca cgagggtttg
    24061 cagaagagga agattagccg gtattacgca tacctgccgc aatcccggca atagtgacca
    24121 ttaacgcttg ttcgacgcga ggatccggtt cctggccttc tttttctgcc tggcgggagc
    24181 ggtgcagcaa ctcggcctgc aatacgttca gcgggtcggt gtaaatattc cgtagctgaa
    24241 tagactctgc aatccacggc agatcggcca tcagatggga atcgttggca atcgccagca
    24301 ccactttgat gtcttcttct tgcaggttgc gtaactcttt acctaacggc cacagtgctt
    24361 tgtctaccag acgttgatca tagtattccg ccaaccacag gtctgctttg gcgaagacca
    24421 tctccagcat gccgagacgc gtcgagaaga atggccaatc gcggcacatg gcttccagct
    24481 cgctctgttt gccatcttcg accacttttt gcagcgccgt acctgcaccc agccaggcgg
    24541 ggagcatcag gcggttttgc gtccaggcaa aaatccacgg tatggcgcgc agcgactcga
    24601 cgccgccggt tgggcgacgt ttcgccggac gtgaacccaa cggcagtttg cccaattctt
    24661 gttccggcgt agcggagcgg aagtaaggca caaaatcttt gttttcacgt acgtagccac
    24721 ggtagagatc gcaggagatg actgacagtt catccataat gcgacgccag ctctctttcg
    24781 gctcaggtgg tggcagcagg ttggcttcca gaatcgcccc ggtgtaaagc gacaggctgc
    24841 tgacggtgat ttctggcaga ccatatttaa agcggatcat ctcgccctgt tcagtcacgc
    24901 gcaggccacc tttcaggctt cctggcggtt gtgacagcag cgccgcatgg gcaggtgcgc
    24961 cgccgcgacc aatggaaccg ccgcgaccgt ggaacaacgt cagctcaata cccgcttttt
    25021 cgcaggtttt gattaatgca tcctgtgcct gatattgcgc ccaggaagct gccatcacgc
    25081 ccgcatcttt tgctgagtcg gaatagccaa tcatcaccat ctgtttgccc tgaatcaggc
    25141 cgcgatacca gtcgatattg agcagctggg tcatgacatc gttggcgttg ttcaggtcat
    25201 cgagggtttc aaacagcgga gcaaccggca tcgcaaaccc gatacccgct tctttcagca
    25261 gcaggtggac agccagtacg tcggacggcg ttttcgccat cgagatcacg taggcggcaa
    25321 tggagccttg cggcgcttcg gcaatcacct ggcaggtatc gagcacttcg cgcgtttcgg
    25381 cgcttggttg ccagttgcgc ggcagaagcg gacgtttgga gttcagttcg cggatcagga
    25441 acgcctgttt gtcggcctct gaccagcttt cgtagtcgcc gataccgagg tagcgggtca
    25501 gctcgcccag cgcttcggta tggcgggtgc tctcctgacg gatatcaata cggaccagcg
    25561 gtacgccgaa acatttcacg cggcgcaggg tgtcgagcag atcgccgttg gcgataatac
    25621 ccatgccaca cgcctgaagt gactggtagc aagcgtagag cggttcccac agttcttcgt
    25681 tttgtgtcag cagcccttct ggttttggca gttcttcgcc tttcaggcgc gcttccagcc
    25741 atgcctgtgt cgccatcagg cgagaacgca ggtttttcat cagatagcga tacggttctg
    25801 cggcaccttc ttcgccaacc agcgccagca gttcaggggt cgcttcaacc atcgacagtt
    25861 cagaaaccag aacctgaata tctttcagga acagatcggt ggctttccag cgactgagta
    25921 gcaggacgtg gcgggtgata tcggcagtga cgttcgggtt gccgtcgcgg tcaccgccca
    25981 tccacgaagt aaaacggacc ggaacaaatt cgacgggcag tttgtagccg aggttctctt
    26041 ccagttgttc gttcagttcg cgcaagtaat tcggtacgcc ttgccacagg ctgttttcta
    26101 ctacggcaaa gccccatttg gcttcatcta ccgggcttgg acgcagctta cggatttcat
    26161 cagtatgcca tgactgggcg attaactggc gcaggcgacg catcagctgg ttgtgttcgt
    26221 agtcagcgat atctttgtta tcgagctgtt ttaagcaggc gttcacttcc accattttgt
    26281 ggatcagtgt acgacgggta atttcggttg ggtgagccgt gaggaccagt tccagcgaca
    26341 gcgattccac tgcttttttg atggtgtctt cgctcagttc cggctggttt ttcagtttgc
    26401 gcagggtgcg ggcgatcact tccgggttgc tggcagcttc gcctttcggc gaaatgctgt
    26461 ggtattgctc ggcggtgttg gctaggttca ggaactgact aaacgcacgc gcaacgggca
    26521 gcagctcgtc gttcgacaaa ttttgtaagg tggtgagcaa ctcctggcgg ttagcatcat
    26581 tgccagcgcg tgaagatttc gacaacttac ggatagtttc tacgcgttca agaatgtgtt
    26641 ctcccaacgc atccttgatg gtttctccca gcactttgcc gagcatactg acattactac
    26701 gcaatgcgga atattgttcg ttcatattac cccagacacc ccatcttatc gtttgatagc
    26761 cctgtatcct tcacgtcgca ttggcgcgaa tatgctcggg ctttgctttt cgtcgtcttt
    26821 tataaagcca cgtaaaagcg gtgacgtcaa atgctgcgaa atcgcttcag caaacgaata
    26881 aatagcagga atttacgcca ttaaattcac gacgctttaa ataagcgtaa cttatggaaa
    26941 tgttaaaaaa tcgccataca taacaccaaa ggtgtaggtc ggataagatg cgcaagcatc
    27001 gcatccgaca ttattgcggc accggagttt ggcaacggtg ccggatgcgg cgcgagcgcc
    27061 ttatccggcc tacgttttaa tgccagcaaa aatggtgaat tacctggata atcaactcac
    27121 gggtgggttt aataaaccgc gtctccagat attcatcagg ctgatgagct tgattgatgg
    27181 aacccggacc gagcaccagc gtcgggcata acgtttgaat aaacggcgct tcggtacagt
    27241 aattcaccac ttcggttttt gctccgagca atttctcaac cacttcaacc agttgatgat
    27301 tcggtgggca ttcatagcca gggatcggcg gatgcagctc gtcgaccgtc agacgacccg
    27361 gccagcgttc gctcaccgga gccaatgcat cgttgagcaa accattaagt tcattgagtg
    27421 tcatgccagg cagcggacga atatccatat gcaactcaca gcaagcgcaa atacggttag
    27481 aagcgtcgcc accgtgaata tgcccgaggt tgagcgtagg gtatggcacg gtaaatgctt
    27541 cgtagtgata acgttctttc aggttatcgc gcaattgcaa aatatgcccg atggcgtcgt
    27601 gcattagttc gatagcgtta actccgcgtg ctggatcgct ggagtgcccc gactggccct
    27661 gaatacggat ggcgttagag atatgacctt tatgtgcgcg taccggttgt agtgacgtcg
    27721 gttcgccaat gatggcgcaa tccgggcgca gggcggtagt ttcggcaaaa taacgcgctc
    27781 cggccatact ggtttcttca tcagcagtcg ccagaatgta gagcggtttt ttcagtttcg
    27841 tgacgtcgac atcgcgtagc gtatcaagga taaacgcaaa aaagcctttc atgtcggcgg
    27901 tgcctaagcc gtaaagcttg ccgtcatgct ccgtcagtgt aaacggatcg cgcgtccagc
    27961 gaccgtcatc aaatggcacc gtatcggtat gccccgccag caacaagccg ccagccccct
    28021 gtccgatact tgccagcata ttgaatttgt tgcgagttcc tggcacaggc tgcacttcca
    28081 cattgaagcc taaatcttta aaccagtccg ccagcagagt gattaaatct gcattgcttt
    28141 gatcgagtgc ctcttccgtg gcgcttattg aaggtgtggc aatcagagca cggtaaatct
    28201 cgataaatgg cggtaatttg tttttcattg ttgacacacc tctggtcatg atagtatcaa
    28261 tattcatgca gtatttatga ataaaaatac actaacgttg agcgtaataa aacccaccag
    28321 ccgtaaggtg aatgttttac gtttaacctg gcaaccagac ataagaaggt gaatagcccc
    28381 gatgttgaat acgctgattg tgggtgccag cggctacgct ggcgcagagc tagtgaccta
    28441 tgtaaatcgc catccgcata tgaacataac cgctttgact gtttcagcgc aaagcaatga
    28501 tgcgggaaag ttaatctccg atttgcatcc gcagctaaaa ggcatcgttg agctgccgtt
    28561 gcagccgatg tcggatatca gcgagtttag cccaggggtg gacgtagtgt ttctcgccac
    28621 cgcccatgaa gttagccacg atttagcgcc gcagtttctt gaagcgggct gcgtggtgtt
    28681 cgacctttcc ggtgcgtttc gtgttaacga cgccaccttc tatgaaaaat attacggctt
    28741 tacccatcaa tacccggaac tgctggaaca ggcagcctac ggtctggcgg agtggtgcgg
    28801 taataaatta aaagaagcga atttgattgc ggtgccaggc tgttatccga cggcggcaca
    28861 gctggcgctg aaaccgttga ttgatgccga tcttcttgac ctcaatcagt ggccggtgat
    28921 caacgccacc agcggcgtga gcggtgcagg acgtaaggca gcgatttcaa atagtttttg
    28981 tgaagttagc ctgcaaccgt atggcgtctt tactcatcgc catcaaccag agatcgccac
    29041 acacctcggt gctgacgtta tcttcacccc acatctgggc aatttcccgc gcggcattct
    29101 cgaaaccatt acctgccgcc tgaaatcggg tgtgacccag gcgcaagtcg cgcaagtgtt
    29161 acagcaggcg tatgcccata aaccgctggt gcggctgtat gacaaaggcg ttccggcgct
    29221 gaaaaatgtc gttgggctgc cattttgcga tatcgggttt gccgttcagg gcgagcatct
    29281 gattattgtg gcgaccgaag acaacttact gaaaggcgcg gcggcacaag cggtacagtg
    29341 cgccaatatt cgtttcggct atgcggaaac gcagtctctt atttaagggt gcaatgatga
    29401 atccattaat tatcaaactg ggcggcgtac tgctggatag tgaagaggcg ctggaacgtc
    29461 tgtttagcac actggtgaat tatcgtgagt cacatcagcg tccgctggtg attgtgcacg
    29521 gcggcggttg cgtggtggat gagctgatga aagggctgaa tctgccggtg aaaaagaaaa
    29581 acggcctgcg ggtgacgcct gctgatcaga tagacattat caccggagca ctggcgggaa
    29641 cggcaaataa aaccctgttg gcatgggcga agaaacatca gattgcggcc gtaggtttgt
    29701 ttctcggtga cggcgacagc gtcaaagtga cccagcttga tgaagagtta ggtcatgttg
    29761 gactggcgca gccaggttcg cctaagctta tcaactcctt gctggagaac ggttatctgc
    29821 cggtggtcag ctccattggc gtaacagacg aagggcaact gatgaacgtc aatgccgacc
    29881 aggcggcaac ggcgctggcg gcaacgctgg gcgcggatct gattttgctc tccgacgtca
    29941 gcggcattct cgacggcaaa gggcaacgca ttgccgaaat gaccgccgcg aaagcagaac
    30001 aactgattga gcagggcatt attactgacg gcatgatagt gaaagtgaac gcggcgctgg
    30061 atgcggcccg cacgctgggc cgtccggtag atatcgcctc ctggcgtcat gcggagcagc
    30121 ttccggcact gtttaacggt atgccgatgg gtacgcggat tttagcttaa gttttgttgg
    30181 ccggaggcgc agctttccgg cattgaattt caaaataagg aaacagagtt atggcacttt
    30241 ggggcgggcg ttttacccag gcagcagatc aacggttcaa acaattcaac gactcactgc
    30301 gctttgatta ccgtctggcg gagcaggata ttgttggctc tgtggcctgg tccaaagccc
    30361 tggtcacggt aggcgtgtta accgcagaag agcaggcgca actggaagag gcgctgaacg
    30421 tgctgctgga agatgttcgc gccaggccac aacaaatcct tgaaagcgac gccgaagata
    30481 tccatagctg ggtggaaggc aaactgatcg acaaagtggg ccagttaggc aaaaagctgc
    30541 ataccgggcg tagtcgtaac gatcaggtag cgactgacct gaaactgtgg tgcaaagata
    30601 ccgttagcga gttactgacg gctaaccgac agctgcaatc ggcgctggtg gaaaccgcac
    30661 aaaacaatca ggacgcggtc atgccaggtt acactcacct gcaacgcgcc cagccggtga
    30721 cgttcgcgca ctggtgcctg gcctatgttg agatgctggc gcgtgatgaa agccgtttgc
    30781 aggatgcgct taagcgtctg gatgtcagcc cattaggctg tggcgcgctg gcgggaacgg
    30841 cctatgaaat cgaccgtgaa cagttagcag gctggctggg ctttgcttcg gcgacccgta
    30901 acagtctgga cagcgtttct gaccgtgacc atgtgttgga actgctgtcg gctgccgcta
    30961 tcggcatggt gcatctgtcg cgttttgctg aagatctgat tttctttaac accggcgaag
    31021 cggggtttgt ggagctttct gaccgcgtga cttccggttc atcattaatg ccgcagaaga
    31081 aaaacccgga tgcgctggag ctgattcgcg gtaaatgcgg tcgggtgcag ggtgcgttaa
    31141 ccggcatgat gatgacgctg aaaggtttgc cgctggctta caacaaagat atgcaggaag
    31201 acaaagaagg tctgttcgac gcgctcgata cctggctgga ctgcctgcat atggcggcgc
    31261 tggtgctgga cggcattcag gtgaaacgtc cacgttgcca ggaagcggct cagcagggtt
    31321 acgccaacgc caccgaactg gcggattacc tggtggcgaa aggcgtacct ttccgcgagg
    31381 cgcaccatat cgtgggtgaa gcggtggtgg aagccattcg tcagggcaaa ccgctggaag
    31441 atctgccgct cgacgagttg cagaaattca gcccggtgat tggcgaagat gtctatccga
    31501 ttttgtcgct gcaatcgtgc ctcgacaagc gtgcggcaaa aggcggcgtc tcaccgcagc
    31561 aggtggccca agcgattgct tttgcgcagg cgcggttggg gtaagaacat ttatatgtac
    31621 aaatttgagc ctggcttatc gccgggcttt tttatggcaa aaaaaagcgg atcctggaga
    31681 tccgcaaaag ttcacgttgg ctttagttat tcgagttgag aaactctcga aacgggcagt
    31741 gacttcaagg gttaaaagag gtgccgctcc gtttctgtga gcaattatca gtcagaatgc
    31801 ttgataggga taatcgttca ttgctattct acctatcgcc atgaactatc gtggcgatgg
    31861 aggatggata atgaatattc gtgatcttga gtacctggtg gcattggctg aacaccgcca
    31921 ttttcggcgt gcggcagatt cctgccacgt tagccagccg acgcttagcg ggcaaattcg
    31981 taagctggaa gatgagctgg gcgtgatgtt gctggagcgg accagccgta aagtgttgtt
    32041 cacccaggcg ggaatgctgc tggtggatca ggcgcgtacc gtgctgcgtg aggtgaaagt
    32101 ccttaaagag atggcaagcc agcagggcga gacgatgtcc ggaccgctgc atattggttt
    32161 gattcccacg gttggaccgt acctgctacc gcatattatc ccgatgctgc accagacctt
    32221 tccaaagctg gaaatgtatc tgcatgaagc acagacccac cagttactgg cgcaactgga
    32281 cagtggcaaa ctcgattgcg tgatcctcgc gctggtgaaa gagagcgaag cattcattga
    32341 agtgccgttg tttgatgagc caatgttgct ggctatctat gaagatcacc cgtgggcgaa
    32401 ccgcgaatgc gtaccgatgg ccgatctggc aggggaaaaa ctgctgatgc tggaagatgg
    32461 tcactgtttg cgcgatcagg caatgggttt ctgttttgaa gccggggcgg atgaagatac
    32521 acacttccgc gcgaccagcc tggagacgct gcgcaacatg gtggcggcag gtagcgggat
    32581 cactttactg ccagcgctgg ctgtgccgcc ggagcgcaaa cgcgatgggg ttgtttatct
    32641 gccgtgcatt aagccggaac cacgccgcac tattggcctg gtttatcgtc ctggctcacc
    32701 gctgcgcagc cgctatgagc agctggcaga ggccatccga gcaagaatgg atggctattt
    32761 cgataaagtg ttaaaacagg cggtttaaac cgtttaacgc agctacccga taggcttccg
    32821 ccatggtcgg gtagttaaag gtggtgttga cgaagtactc aatagtgttg ccgccacctt
    32881 tctgttccat aatcgcctga ccgatatgaa taatttcggc agcgcgctcg ccaaagcagt
    32941 gaatacccag aatctctttt gtttcccgat ggaacaaaat tttcagcgtg cccacgttca
    33001 tgccgacgat ttgtgcgcgt gccagatgtt taaactgggc gcggcccact tcatatggca
    33061 ctttcatcgc ggtcagctgc tgttcggttt tgcccacaga gctgatttcc gggatggtgt
    33121 aaatgccggt agggatatct tcaatcagat gcgcggtggc ttcgcctttt accagcgcct
    33181 gcgcggcaat gcgcccctgg tcataggccg ctgatgccag gctcggataa ccaatcacgt
    33241 cgcccaccgc gtaaacgtgc ggctgtgcgg tctgatacat gctgttgacc ttcagctgtc
    33301 cgcgactgtc agtttccagc ccaatgttct gtaacgccag cgaatcggta ttaccggtac
    33361 gaccgttggc atagagcagg cagtcagctt tcagtttttt acccgacttc agatgcatga
    33421 tcacaccgtc gtcacagcct tcgatcttct cgtactcttc gttgtgacga atcactacgc
    33481 cactgttcca gaagtgatag gagagagaat ctgacatctc ttgatcgaga aatgccagca
    33541 ggcgatcgcg ggtgttgatc agatccactt ttacatccat accgcggaag atcgacgcat
    33601 attcacagcc gatcactcca gcaccataga taagtacatg gcgcggttcg tggtgcatgc
    33661 taagaattga gtcgctgtcg taaatgcgtg gatgggtgaa atcaacatct gttggatgat
    33721 atggacgaga gccgcaggca ataacaaatt tttcagcggt tagtgtttcg acgctgccgt
    33781 ccgggcaatc cagcgccaac gtatgctcgt caacaaagcg agcgttcccc tgcaatattt
    33841 cacagtgatt acgttcgtaa aatccctgac gcatgcgcgt ttgttgatta atcacgttat
    33901 cggcatggtt aaggatatcg gcaaaagaag agcggagcag tcgcgaatgg tcgctgtata
    33961 gtgggttttg attgaattct ataatgcggc tgacggcgtg acggagagct ttcgacggga
    34021 tggtgcccca gtgggtgcaa ccgccgccaa cattttgata acgctcgata actgcgacgc
    34081 gcacaccttg cttaaccagg cccattgcag cgccttcgcc gccggggccg gaacctatta
    34141 ctatggcatc gtaatcgtag gaatgtggca tggtagggct tacctgttct tatacataaa
    34201 agcaacagaa tggtaacatt ctatcgcggg taagccaatt gatccccgtc atttatctgg
    34261 ctatatcctg agcggccttt gctttgtctg tttcttactt ttgccctgac gttttattgg
    34321 atttttatcg acgatactct ccgtttaagc ggcaggtttc cgctgtacgt aaaagaaccg
    34381 gccagtaaat atgttttatt gcgttaccgt tcattcacaa tactggagca atccagtatg
    34441 ttcattctct ggtatagtgc cagcagtact tttggcaagg attcagacat cgtgatgggc
    34501 ataagagcgc aacaaaaaga aaaaacccgc cgttcgctgg tggaagccgc atttagccaa
    34561 ttaagtgctg aacgcagctt cgccagcctg agtttgcgtg aagtggcgcg tgaagcgggc
    34621 attgctccca cctcttttta tcggcatttc cgcgacgtag acgaactggg tctgaccatg
    34681 gttgatgaga gcggtttaat gctacgccaa ctcatgcgcc aggcgcgtca gcgtatcgcc
    34741 aaaggcggga gtgtgatccg cacctcggtc tccacattta tggagttcat cggtaataat
    34801 cctaacgcct tccggttatt attgcgggaa cgctccggca cctccgctgc gtttcgtgcc
    34861 gccgttgcgc gtgaaattca gcacttcatt gcggaacttg cggactatct ggaactcgaa
    34921 aaccatatgc cgcgtgcgtt tactgaagcg caagccgaag caatggtgac aattgtcttc
    34981 agtgcgggtg ccgaggcgtt ggacgtcggc gtcgaacaac gtcggcaatt agaagagcga
    35041 ctggtactgc aactgcgaat gatttcgaaa ggggcttatt actggtatcg ccgtgaacaa
    35101 gagaaaaccg caattattcc gggaaatgtg aaggacgagt aatgaaacaa gcaaatcaag
    35161 atagaggtac gctgctgctg gcgttagttg ctggcttatc gattaatggt actttcgcag
    35221 cgctgtttag ctccattgtg ccattttctg tattcccgat tatttccctg gtgctgacgg
    35281 tttactgcct gcatcaacgt tatcttaatc gcactatgcc ggtaggcttg ccgggtctgg
    35341 cagctgcctg ttttattctc ggcgtactgc tgtacagcac ggtagttcgt gcggaatatc
    35401 cggatatcgg ctctaacttc ttcccggcag tactctccgt cattatggtg ttctggattg
    35461 gcgcgaagat gcgtaaccgt aagcaggaag ttgctgagta atcggtatta tgccggatgc
    35521 tggcgcatcc ggcatgggtt ttacttcgcg gtcagtaata cgccgcactc catatggtgc
    35581 gtgtagggga actgatcaaa cagagccaga cgttcgacct tgtgcgtctg gcttaatgtt
    35641 tccagattct tgcataacgt ttccgggtta caggagatgt acaaaataca cggatacgcc
    35701 tgcaccattt tctcggtttc actgtccaga ccgctgcgcg gtgggtcgac aaaaatggtt
    35761 tcgcactgat aactctttaa gtcgatccct tgcaggcggt taaactcgcg cacaccattc
    35821 atcgcctgag taaattcttc tgccgccata cgaataattt gtacgttatc aatatggtta
    35881 gctgcgatgt tgtattgcgc agcagcaacc gacggcttag cgatttcggt ggctaatacc
    35941 cgatcaaaat tgcgcgccag cgctaatgaa aagttaccgt tgccgcagta cagctccagt
    36001 aaatcgcctt ttgagccttt ggttacgtcc agcgcccatt ccagcatctg aatattcatc
    36061 gccgcgttcg gctgggtaaa gctgttttct acctgacggt agatcatctc tttccctgcg
    36121 accggcagac gctcgtcgat gtaatcttga tccagctcga ttttggtttt cgttgcccga
    36181 ccaatcagat gcacattcag attctgcgcg cgcagtgcat cgcgcagggc ctccgcttcc
    36241 tgacgccact catcatccag cttcttatgg tatagcaggg aaaccaccgc ctgattactc
    36301 agcgtggtga ggtaatcaat ctggaacaac ttgtggcgca gaacgggatt attacgcaca
    36361 cccgcaatca tcgccgtcat caactggttg ataagttcac tggcggcggg gaagctatcc
    36421 acgcggatac ggcttttggt ttgttgatcg aaaatgatgt gatacaggtc atcgccatcg
    36481 tgccagatgc ggaactccgc acgcatccgg taatgactga ccggcgagcg aaacacttcc
    36541 ggaaccaggt cagaaaacgg tgccatcata ctttgcaaac gtaccacttt ttcggctaac
    36601 tgcgcttcat actgttctgt tggaaggtgt tcgggggtca tgatgtatcc tgaaaaatta
    36661 aagtacgcgg cgattgtagg gattgctcat cagatgtcca gatcttgatg aattcctatt
    36721 tgtgagctac gtctggacag taacttgtta caacctgtag catccacttg tcggtcctgt
    36781 gagttaatag ggaatccagt gcgaatctgg agctgacgcg cagcggtaag gaaaggtgcg
    36841 atgattgcgt tatgcggaca ctgccatccg gtgggaagtc atcatctctt agtatcttag
    36901 atacccctcc aagcccgaag acctgccggc caacgtcgca tctggttctc atcatcgcgt
    36961 aatattgatg aaacctgcgg catccttctt ctattgtgga tgctttacaa tgattaaaaa
    37021 agcttcgctg ctgacggcgt gttctgtcac agccttttcc gcttgggcac aggataccag
    37081 cccggatact ctcgtcgtta ctgctaaccg ttttgaacag ccgcgcagca ctgtgcttgc
    37141 accaaccacc gttgtgaccc gtcaggatat cgaccgctgg cagtcgacct cggtcaatga
    37201 tgtgctgcgc cgtcttccgg gcgtcgatat cacccaaaac ggcggttcag gtcagctctc
    37261 atctattttt attcgcggta caaatgccag tcatgtgttg gtgttaattg atggcgtacg
    37321 cctgaatctg gcgggggtga gtggttctgc cgaccttagc cagttcccta ttgcgcttgt
    37381 ccagcgtgtt gaatatatcc gtgggccgcg ctccgctgtt tatggttccg atgcaatagg
    37441 cggggtggtg aatatcatca cgacgcgcga tgaacccgga acggaaattt cagcagggtg
    37501 gggaagcaat agttatcaga actatgatgt ctctacgcag caacaactgg gggataagac
    37561 acgggtaacg ctgttgggcg attatgccca tactcatggt tatgatgttg ttgcctatgg
    37621 taatacagga acgcaagcgc agacagataa cgatggtttt ttaagtaaaa cgctttatgg
    37681 cgcgctggag cataacttta ctgatgcctg gagcggcttt gtgcgcggct atggctatga
    37741 taaccgtacc aattatgacg cgtattattc tcccggttca ccgttgctcg atacccgtaa
    37801 actctatagc caaagttggg acgccgggct gcgctataac ggcgaactga ttaaatcaca
    37861 actcattacc agctatagcc atagcaaaga ttacaactac gatccccatt atggtcgtta
    37921 tgattcgtcg gcgacgctcg atgagatgaa gcaatacacc gtccagtggg caaacaatgt
    37981 catcgttggt cacggtagta ttggtgcggg tgtcgactgg cagaaacaga ctacgacgcc
    38041 gggtacaggt tatgttgagg atggatatga tcaacgtaat accggcatct atctgaccgg
    38101 gctgcaacaa gtcggcgatt ttacctttga aggcgcagca cgcagtgacg ataactcaca
    38161 gtttggtcgt catggaacct ggcaaaccag cgccggttgg gaattcatcg aaggttatcg
    38221 cttcattgct tcctacggga catcttataa ggcaccaaat ctggggcaac tgtatggctt
    38281 ctacggaaat ccgaatctgg acccggagaa aagcaaacag tgggaaggcg cgtttgaagg
    38341 cttaaccgct ggggtgaact ggcgtatttc cggatatcgt aacgatgtca gtgacttgat
    38401 cgattatgat gatcacaccc tgaaatatta caacgaaggg aaagcgcgga ttaagggcgt
    38461 cgaggcgacc gccaattttg ataccggacc actgacgcat actgtgagtt atgattatgt
    38521 cgatgcgcgc aatgcgatta ccgacacgcc gttgttacgc cgtgctaaac agcaggtgaa
    38581 ataccagctc gactggcagt tgtatgactt cgactggggt attacttatc agtatttagg
    38641 cactcgctat gataaggatt actcatctta tccttatcaa accgttaaaa tgggcggtgt
    38701 gagcttgtgg gatcttgcgg ttgcgtatcc ggtcacctct cacctgacag ttcgtggtaa
    38761 aatagccaac ctgttcgaca aagattatga gacagtctat ggctaccaaa ctgcaggacg
    38821 ggaatacacc ttgtctggca gctacacctt ctgaaccacg tcccaccgtg ctggtgtttg
    38881 actccggcgt cggtgggttg tcggtctatg acgagatccg gcatctctta ccggatctcc
    38941 attacattta tgctttcgat aacgtcgctt tcccgtatgg cgaaaaaagc gaagcgttta
    39001 ttgttgagcg agtggtggca attgtcaccg cggtgcaaga acgttatccc cttgcgctgg
    39061 ctgtggtcgc ttgcaacact gccagtaccg tttcacttcc tgcattacgc gaaaagttcg
    39121 acttcccggt tgttggtgtc gtgccggcga ttaaacctgc tgcacgtctg acggcaaatg
    39181 gcattgtcgg attactggca acccgcggaa cagttaaacg ttcttatact catgagctga
    39241 tcgcgcgttt cgctaatgaa tgccagatag aaatgctggg ctcggcagag atggttgagt
    39301 tggctgaagc gaagctacat ggcgaagatg tttctctgga tgcactaaaa cgtatcctac
    39361 gcccgtggtt aagaatgaaa gagccgccag ataccgttgt attgggttgc acccatttcc
    39421 ctctactaca agaagaactg ttacaagtgc tgccagaggg aacccggctg gtggattctg
    39481 gcgcagcgat tgctcgccga acggcctggt tgttagaaca tgaagccccg gatgcaaaat
    39541 ctgccgatgc gaatattgcc ttttgtatgg caatgacgcc agaagctgaa caattattgc
    39601 ccgttttaca gcgttacggc ttcgaaacgc tcgaaaaact ggcagtttta ggctgatttg
    39661 gttgaatg
//
LOCUS       DAZRUF010000012        38337 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZRUF010000012 DAZRUF010000000
VERSION     DAZRUF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.012"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHXOL_000503"
     CDS             59..1423
                     /locus_tag="ACHXOL_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6807572.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFHLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHXOL_000504"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHXOL_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR6807573.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHXOL_000505"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHXOL_000505"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR6807574.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="ACHXOL_000506"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="ACHXOL_000506"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR6807575.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHXOL_000507"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHXOL_000507"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR6807576.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="ACHXOL_000508"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="ACHXOL_000508"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR6807577.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="ACHXOL_000509"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="ACHXOL_000509"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR6807578.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="ACHXOL_000510"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="ACHXOL_000510"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR6807579.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="ACHXOL_000511"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="ACHXOL_000511"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR6807580.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHXOL_000512"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHXOL_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR6807581.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHXOL_000513"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHXOL_000513"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR6807582.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHXOL_000514"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHXOL_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR6807583.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHXOL_000515"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHXOL_000515"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR6807584.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHXOL_000516"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHXOL_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR6807585.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHXOL_000517"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHXOL_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR6807586.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="ACHXOL_000518"
     CDS             13749..14288
                     /locus_tag="ACHXOL_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR6807587.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="ACHXOL_000519"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="ACHXOL_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR6807588.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHXOL_000520"
     CDS             complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHXOL_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR6807589.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHXOL_000521"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHXOL_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR6807590.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHXOL_000522"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHXOL_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR6807591.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHXOL_000523"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHXOL_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR6807592.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHXOL_000524"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHXOL_000524"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR6807593.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHXOL_000525"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHXOL_000525"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR6807594.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="ACHXOL_000526"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="ACHXOL_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR6807595.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHXOL_000527"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHXOL_000527"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR6807596.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHXOL_000528"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHXOL_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR6807597.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHXOL_000529"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHXOL_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR6807598.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHXOL_000530"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHXOL_000530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR6807599.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHXOL_000531"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHXOL_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR6807600.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHXOL_000532"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHXOL_000532"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR6807601.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHXOL_000533"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHXOL_000533"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR6807602.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLTLENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="ACHXOL_000534"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="ACHXOL_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR6807603.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHXOL_000535"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHXOL_000535"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR6807604.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHXOL_000536"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHXOL_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR6807605.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="ACHXOL_000537"
     CDS             complement(37465..37920)
                     /locus_tag="ACHXOL_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6807606.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="ACHXOL_000538"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="ACHXOL_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9465 a        10314 c         9734 g         8824 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttcatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacgc ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcta
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ccgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactgacgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaagcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggac gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAZRUF010000013        35403 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZRUF010000013 DAZRUF010000000
VERSION     DAZRUF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.013"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            63..347
                     /gene="pduA"
                     /locus_tag="ACHXOL_000539"
     CDS             63..347
                     /gene="pduA"
                     /locus_tag="ACHXOL_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR6807607.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            344..1156
                     /gene="pduB"
                     /locus_tag="ACHXOL_000540"
                     /pseudo
     CDS             344..1156
                     /gene="pduB"
                     /locus_tag="ACHXOL_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
     gene            1175..2839
                     /gene="pduC"
                     /locus_tag="ACHXOL_000541"
     CDS             1175..2839
                     /gene="pduC"
                     /locus_tag="ACHXOL_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR6807608.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2849..3517
                     /locus_tag="ACHXOL_000542"
     CDS             2849..3517
                     /locus_tag="ACHXOL_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR6807609.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3532..4050
                     /gene="pduE"
                     /locus_tag="ACHXOL_000543"
     CDS             3532..4050
                     /gene="pduE"
                     /locus_tag="ACHXOL_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR6807610.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4060..5892
                     /locus_tag="ACHXOL_000544"
     CDS             4060..5892
                     /locus_tag="ACHXOL_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR6807611.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            5882..6232
                     /locus_tag="ACHXOL_000545"
     CDS             5882..6232
                     /locus_tag="ACHXOL_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR6807612.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            6252..6527
                     /gene="pduJ"
                     /locus_tag="ACHXOL_000546"
     CDS             6252..6527
                     /gene="pduJ"
                     /locus_tag="ACHXOL_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR6807613.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6551..6973
                     /locus_tag="ACHXOL_000547"
     CDS             6551..6973
                     /locus_tag="ACHXOL_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR6807614.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            6976..7608
                     /locus_tag="ACHXOL_000548"
     CDS             6976..7608
                     /locus_tag="ACHXOL_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR6807615.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7605..8096
                     /gene="pduM"
                     /locus_tag="ACHXOL_000549"
     CDS             7605..8096
                     /gene="pduM"
                     /locus_tag="ACHXOL_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR6807616.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8100..8375
                     /gene="pduN"
                     /locus_tag="ACHXOL_000550"
     CDS             8100..8375
                     /gene="pduN"
                     /locus_tag="ACHXOL_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR6807617.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8387..9394
                     /gene="pduO"
                     /locus_tag="ACHXOL_000551"
     CDS             8387..9394
                     /gene="pduO"
                     /locus_tag="ACHXOL_000551"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR6807618.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9391..10776
                     /gene="pduP"
                     /locus_tag="ACHXOL_000552"
                     /pseudo
     CDS             9391..10776
                     /gene="pduP"
                     /locus_tag="ACHXOL_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            10787..11899
                     /locus_tag="ACHXOL_000553"
     CDS             10787..11899
                     /locus_tag="ACHXOL_000553"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR6807619.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11896..13239
                     /locus_tag="ACHXOL_000554"
     CDS             11896..13239
                     /locus_tag="ACHXOL_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR6807620.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARADTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13242..13796
                     /gene="pduT"
                     /locus_tag="ACHXOL_000555"
     CDS             13242..13796
                     /gene="pduT"
                     /locus_tag="ACHXOL_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR6807621.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13796..14146
                     /gene="pduU"
                     /locus_tag="ACHXOL_000556"
     CDS             13796..14146
                     /gene="pduU"
                     /locus_tag="ACHXOL_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR6807622.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14151..14594
                     /locus_tag="ACHXOL_000557"
     CDS             14151..14594
                     /locus_tag="ACHXOL_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR6807623.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(14695..15024)
                     /locus_tag="ACHXOL_000558"
     CDS             complement(14695..15024)
                     /locus_tag="ACHXOL_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR6807624.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15196..16254)
                     /locus_tag="ACHXOL_000559"
     CDS             complement(15196..16254)
                     /locus_tag="ACHXOL_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR6807625.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16452..16925)
                     /gene="sbmC"
                     /locus_tag="ACHXOL_000560"
     CDS             complement(16452..16925)
                     /gene="sbmC"
                     /locus_tag="ACHXOL_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR6807626.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17044..18210)
                     /gene="dacD"
                     /locus_tag="ACHXOL_000561"
     CDS             complement(17044..18210)
                     /gene="dacD"
                     /locus_tag="ACHXOL_000561"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR6807627.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18419..19846
                     /gene="sbcB"
                     /locus_tag="ACHXOL_000562"
     CDS             18419..19846
                     /gene="sbcB"
                     /locus_tag="ACHXOL_000562"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR6807628.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(19889..20116)
                     /gene="tsuB"
                     /locus_tag="ACHXOL_000563"
     CDS             complement(19889..20116)
                     /gene="tsuB"
                     /locus_tag="ACHXOL_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR6807629.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(20130..21188)
                     /gene="tsuA"
                     /locus_tag="ACHXOL_000564"
     CDS             complement(20130..21188)
                     /gene="tsuA"
                     /locus_tag="ACHXOL_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR6807630.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(21367..22725)
                     /gene="plaP"
                     /locus_tag="ACHXOL_000565"
     CDS             complement(21367..22725)
                     /gene="plaP"
                     /locus_tag="ACHXOL_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR6807631.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(22715..22777)
                     /gene="yoeI"
                     /locus_tag="ACHXOL_000566"
     CDS             complement(22715..22777)
                     /gene="yoeI"
                     /locus_tag="ACHXOL_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR6807632.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(22992..23921)
                     /locus_tag="ACHXOL_000567"
     CDS             complement(22992..23921)
                     /locus_tag="ACHXOL_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR6807633.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(23967..24791)
                     /locus_tag="ACHXOL_000568"
     CDS             complement(23967..24791)
                     /locus_tag="ACHXOL_000568"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR6807634.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(24874..25128)
                     /gene="yoeB"
                     /locus_tag="ACHXOL_000569"
     CDS             complement(24874..25128)
                     /gene="yoeB"
                     /locus_tag="ACHXOL_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR6807635.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(25125..25376)
                     /gene="yefM"
                     /locus_tag="ACHXOL_000570"
     CDS             complement(25125..25376)
                     /gene="yefM"
                     /locus_tag="ACHXOL_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR6807636.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            25659..25709
                     /gene="hisL"
                     /locus_tag="ACHXOL_000571"
     CDS             25659..25709
                     /gene="hisL"
                     /locus_tag="ACHXOL_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR6807637.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    25685..25808
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            25855..26754
                     /gene="hisG"
                     /locus_tag="ACHXOL_000572"
     CDS             25855..26754
                     /gene="hisG"
                     /locus_tag="ACHXOL_000572"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR6807638.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            26760..28064
                     /gene="hisD"
                     /locus_tag="ACHXOL_000573"
     CDS             26760..28064
                     /gene="hisD"
                     /locus_tag="ACHXOL_000573"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR6807639.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            28061..29131
                     /gene="hisC"
                     /locus_tag="ACHXOL_000574"
     CDS             28061..29131
                     /gene="hisC"
                     /locus_tag="ACHXOL_000574"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR6807640.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            29131..30198
                     /gene="hisB"
                     /locus_tag="ACHXOL_000575"
     CDS             29131..30198
                     /gene="hisB"
                     /locus_tag="ACHXOL_000575"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR6807641.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            30198..30788
                     /gene="hisH"
                     /locus_tag="ACHXOL_000576"
     CDS             30198..30788
                     /gene="hisH"
                     /locus_tag="ACHXOL_000576"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR6807642.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            30788..31525
                     /gene="hisA"
                     /locus_tag="ACHXOL_000577"
     CDS             30788..31525
                     /gene="hisA"
                     /locus_tag="ACHXOL_000577"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR6807643.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            31507..32283
                     /gene="hisF"
                     /locus_tag="ACHXOL_000578"
     CDS             31507..32283
                     /gene="hisF"
                     /locus_tag="ACHXOL_000578"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR6807644.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            32277..32888
                     /gene="hisIE"
                     /locus_tag="ACHXOL_000579"
     CDS             32277..32888
                     /gene="hisIE"
                     /locus_tag="ACHXOL_000579"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR6807645.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(32985..33962)
                     /gene="wzzB"
                     /locus_tag="ACHXOL_000580"
     CDS             complement(32985..33962)
                     /gene="wzzB"
                     /locus_tag="ACHXOL_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR6807646.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(34108..35274)
                     /gene="ugd"
                     /locus_tag="ACHXOL_000581"
     CDS             complement(34108..35274)
                     /gene="ugd"
                     /locus_tag="ACHXOL_000581"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR6807647.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         9079 a         9201 c         8840 g         8283 t
ORIGIN      
        1 ccattctttt tcctcgcttt tttttatagt catatcgtca aaaaaatcca ggcgaggtct
       61 ttatgcaaca agaagcgtta ggaatggtag aaaccaaagg cttaactgcc gccatagagg
      121 ccgcagacgc catggtgaag tcggcaaatg ttttgctggt cggctacgaa aaaataggtt
      181 ccgggctggt gaccgtcatc gttcgtggtg atgtcggggc ggtaaaagcg gcaacagacg
      241 cgggtgccgc agctgcgcat aacgtcggcg aagtaaaagc cgtacacgtt attccccgcc
      301 cacataccga tgtcgaaaaa atcttaccaa agggaattaa cccatgagca gcaatgaact
      361 ggtcgaacag atcatggcgc aggtcatcgc tcgcgtagcg acgccagagc atgaggttct
      421 cccgaaaacc actcatcaaa tacgatagac ggctatggca gaaaaaagct gcacgctaac
      481 agaatttgtt ggcaccgcta ttggcgacac tgtaggtctg gtcattgcga acgttgatag
      541 cgccctgttg gacgcgatga aacttgaaaa acgctatcgt tccattggca ttctgggtgc
      601 gcgtattggc gcagggccac acattatggc cgctgatgaa gcggtaaaag ccaccaacac
      661 tgaagttatt agcattgaac ttcctcgcga tactaaaggc ggtgccggac acggctcact
      721 gatcctctta ggtggcaacg atgtttccga cgtgaaacgc ggtattgaag tcgccctgaa
      781 agaactcgat cggacctttg gcgatgtcta tgccaacgaa gccggacatc ttgaactgca
      841 atataccgcc cgcgccagct atgcactgga aaaagcgttt ggtgcaccac tgggacgggc
      901 ttgcggcgtt attgtcggcg ctccggctgc tgtgggtgtt ctgatggccg acaccgcgct
      961 gaagtccgcc aatgtggatg ttgtggcata cagctcccca gctcaaggca ccagctacag
     1021 caatgaagtc attctgatta tctcgggtga ctccggcgca gtccgccagg cggtcatctc
     1081 cgcacgcgaa atcggtaaaa ccgtgctttc gactctcggc gatacaccga aaaacgatca
     1141 cccttcctac atctgaattc tctgaggcag atttatgaga tcgaaaagat ttgaagcact
     1201 ggcaaaacgc ccggtcaacc aggacggttt cgttaaagag tggattgaag aaggctttat
     1261 tgcgatggaa agcccgaacg atcctaaacc gtcaatcaaa attgttaacg gcgtcgttac
     1321 cgaactggat ggaaaacccg ccagccagtt tgacctgatc gaccacttta tcgcccgcta
     1381 cggcatcaat ctcgcgcgcg ccgaagaagt catcgcgatg gattcggtaa aacttgccaa
     1441 tatgttgtgc gatccaaatg tcaaacgtag cgatatcgtt ccccttacca cagcaatgac
     1501 gcctgcaaaa atcgtcgaag tggtttcgca gatgaacgtg gtagagatga tgatggcgat
     1561 gcagaaaatg cgcgcccgtc gaacaccatc acaacaggct cacgtcacca acgtcaaaga
     1621 taacccagta caaattgccg ccgacgcagc tgaaggcgca tggcgcgggt ttgatgaaca
     1681 ggaaaccacc gttgccgttg ctcgctatgc accgttcaac gccatcgccc tgctggtcgg
     1741 ctcacaggtg ggacgtccgg gcgtcttgac ccagtgttca ctggaagaag ccaccgaact
     1801 gaaactcggt atgttaggcc acacctgtta cgccgaaaca atttctgtct atggtacaga
     1861 acctgttttc accgatggtg atgatacccc gtggtcaaaa gggtttctcg cgtcatccta
     1921 tgcttctcgt ggcttaaaaa tgcgctttac ttccggctcc ggctctgaag tacagatggg
     1981 ttacgcggaa ggcaaatcaa tgctttacct cgaggctcgt tgcatttaca tcaccaaagc
     2041 cgcaggcgtt cagggtctgc agaatggttc ggtaagctgc atcggtgttc catcagctgt
     2101 gccatcaggc atccgcgcag tattagcgga aaacttaatt tgctcatcgc tggatctgga
     2161 atgcgcctcc agtaatgacc agacctttac ccactcggat atgcgccgta ccgcacgttt
     2221 cctgatgcaa tttctgccag gcaccgactt tatctcttcc ggtttttccg ctgtgccgaa
     2281 ctacgacaac atgtttgctg gttcaaacga agatgctgaa gacttcgatg attacaacgt
     2341 cattcagcgt gacctgaagg ttgatggtgg gctgcgacca gtacgcgaag aagatgttat
     2401 cgctatccgc aataaagccg ctcgcgcttt acaggctgtc tttgccggta tgggactacc
     2461 gcctattacc gatgaagaag tcgaagcggc aacctatgcc cacggttcaa aagatatgcc
     2521 tgagcgcaat attgtggaag acatcaagtt cgcccaggag attatcaata aaaaccggaa
     2581 cagcctggaa gtggtgaaag ccttggcgca aggcggtttt accgatgtcg cccaggacat
     2641 gctcaacatg caaaaagcaa aactgaccgg cgattatctc catacctcag ccatcatcgt
     2701 cgatgacgga caagtgctct ctgcggtcaa tgacgtcaat gattacgccg gaccggctac
     2761 aggttaccgc ctgcaagggg aacgctggga agagatcaaa aatattcccg gtgcacttga
     2821 tcccaacgaa attgactaag ggtgagaaat ggaaattaac gaaaaattac tgcgtcagat
     2881 tatcgaagac gtgctggcag aaatgcaacc cagcgacaag tccgtttcat ttcgcgcacc
     2941 tgtctctgca actgtccctt ccgcgccaga taccggaaat tttctgaccg aaattggcga
     3001 agcccaacaa ggcactcagc aagatgaagt cattattgct gtaggcccgg cgttcggcct
     3061 ggcgcaaacc gtcaatatca tcggtattcc gcataaaaac atcttgcgcg aagtgattgc
     3121 cggtatcgaa gaagaaggca ttaaagcccg tgtaattcgc tgctttaaat cgtccgatgt
     3181 cgcattcgta gcagtcgaag gcgatcgtct tagtggttca ggtattgcta tcggtattca
     3241 gtcgaaaggc acaacggtta tccaccagca gggacttcct cccttgtcta acctggaact
     3301 tttcccacaa gcgcctttgt tgacgctgga tacttatcgt cagattggca aaaacgccgc
     3361 gcgttatgcg aagcgcgagt ctccacaacc ggtgccgacc ctcaacgatc aaatggcgcg
     3421 gccgaaatac caggccaaat ctgccatttt gcatattaaa gagacgaagt acgtggtcac
     3481 tggcaaaaaa ccgcaagaac tgcgcgtgac gttttaacaa aggaaacctt catgaatacc
     3541 gatgcaattg agtcgatggt cagggacgtg ttgaaccgca tgaacagttt acaggatgca
     3601 gctcctgttt ctgcggtccc caacgcatca atactgagcg ccaaagtgac ggattacccg
     3661 ctggcaaata aacatccgga atgggttaaa acagcgacca ataaaacgct cgacgatttc
     3721 actctggaaa atgtgctaag cgataacgtc accgccctgg atatgcgcat tacccctgaa
     3781 acattgcgta ttcaggctgc gattgccaga gatgctggtc gcgatcgtct ggcgatgaat
     3841 tttgaacgcg ccgctgagct tacctcagta ccagatgatc gcatcctcga aatctacaat
     3901 gcgttacgcc cttatcgttc aactaagcaa gaacttattg ccattgctga cgatcttgaa
     3961 cagcgttatc aggccaaaat ttgtgctgca tttgtccgtg aagctgcgga actttatgtc
     4021 gagcgcaaaa aactgaaagg cgacgattaa ggacgaacta tgcgatatat agcaggcatt
     4081 gatatcggca actcatcaac agaggttgcg ctggcaacgc tcagtgcaac tggagagttg
     4141 tcgttcgtga gtagcgccct ggaggaaacc accggaatca aaggtacgtt gcgtaacgtc
     4201 catggtattc aggaggcgct cgcacaggca acgaaaaaag tcggcatcaa tgttagcgat
     4261 atttcgctta ttcgtatcaa cgaagcaact ccggtcattg gtgatgtggc gatggaaacc
     4321 atcactgaaa ctatcatcac cgaatcaacc atgatcggtc acaacccgaa aactccgggc
     4381 ggagtggggc tgggcgtagg gttcaccatc actccgcaag agttattaac acgcccggcg
     4441 gacacacctt acatcctggt ggtgtcatcg gcatttgatt ttgccgatat cgcgacgatg
     4501 ataaacgcct ccgtacgcgc cggatatcag ctgactggcg ttattctgca acgcgacgat
     4561 ggcgtactgg tcaataaccg cctggaaatt cctttaccta ttgtggatga agtcctctac
     4621 atcgatcgta ttccgctcgg catgctggca gcaattgaag ttgccgttcc cgggaaagtt
     4681 atcgaaaccc tgtctaaccc gtatgggatt gccaccgtat tcgccctcaa tgctgaagag
     4741 actaaaaata ttgttcctgt cgcgcgggcg ctaatcggca accgttcagc agtggtagtg
     4801 aaaacgccct ctggcgacgt taaagcccgt tcgatacccg ccggaaatat cgaactgctt
     4861 tctgccgggc gcacaacgcg cgttgatgta gctgctggtg ccgatgccat tatgaaagcg
     4921 gtcggtgagt gcccaaaact ggaaaacgtg acgggagaac caggcaccaa catcggcgga
     4981 atgctggaac atgttcgcca gaccatggcg gaactgacca acaaacccag caacgaaatt
     5041 tttattcagg atttgctggc gatagatacc tctgtccccg tcagcgtaac cggtggtctg
     5101 gcaggtgaat tttcactgga acaggctgtg ggtattgcct ctatggtgaa atccgaccgt
     5161 ctgcaaatgg cgatgatcgc cagtgaaatt aagcaaaaac tgcatgttga tgttcaggtc
     5221 ggaggcgcag aagcagaagc cgccattcag ggggccttga ctacccctgg aaccacacgt
     5281 ccactggcaa ttctggactt aggtgcaggc tctaccgatg cctccatcat caatcagagt
     5341 ggggaaatcg ttgccactca ccttgcggga gcgggcgaca tggtaacgat gattatcgct
     5401 cgtgagctgg gcttaaatga tcgttatctg gcagaagaaa tcaaaaagta cccgttagca
     5461 aaagtagaaa gcctgttcca cttacgccat gaagacggta gcgttcagtt cttccctact
     5521 cccctatccc cccatgtatt tgcccgcgtc tgtgtagtga aacccgacga actggtgcct
     5581 attcccggtg atttgacgct ggaaaaagta cgcgctgttc gtcgcagtgc gaaagagcgt
     5641 gtcttcgtaa ccaatgccct gcgcgcgcta cgtcaggtaa gccctgccgg aaatattcgc
     5701 gacattccgt tcgtcgttct ggtgggtggt tcttcgctgg atttcgaagt accgcaattg
     5761 gtcaccgacg cgctggcaca ttatcgactg gtcgccgggc gggggaatat ccgtggtagc
     5821 gaaggtccgc gcaacgcggt agctactggg ctactcatcg cctggcacaa ggagtcgatt
     5881 catggaaagt aaagagcgtg caccagccat cgttgtcatg gagatcggtg actgtatcac
     5941 tacatgggat gaggtgcttc ttggcattga agaggaaggt attccctttc gcattcaaca
     6001 cattccgtca ggtgaagtta tcgatagcgc ctggctggct gctcgtcaat ctcccctgtt
     6061 ggttggcatt gcctgcgacc gggaagaact gattgtgcat tacaaaaact taccggcatc
     6121 agcgccactg tttacgctga cgtatcaaca agataaccat acccggcgca gcatcggcac
     6181 taacgctgcc cgcctggtaa aaggaattcc cttccgggaa ttccactcat aatccacagg
     6241 agaaacgctg tatgaacaac gcactaggac tggttgaaac aaaagggtta gttggcgcaa
     6301 ttgaagctgc agatgccatg gttaaatccg ccaacgtgca gctggttggc tatgaaaaga
     6361 ttggttcggg tctgattacg gtgatggtcc gcggtgatgt tggcgcagta aaggccgccg
     6421 ttgatgcggg gagcgctgcg gcaagcgccg ttggcgaagt gaaatcctgc catgtcatcc
     6481 cgcgcccaca cagtgatgtt gaggctattt tacctaaatc ggcataaccc catttgctaa
     6541 ggagcacagc gtgaagcaat cactgggatt acttgaagcc agtggtctgg ctctcgccat
     6601 cagttgcgca gacgctatgg caaaagccgc gtccatcacg ctggttggcc ttgagaagac
     6661 caatggctca ggctggacgc tcatcaaaat caccggtgac gtcgcctcgg ttaatgcggc
     6721 gatcgcgacg ggagccagtt tcgccggaca gcgaaatggc cttgtcgcac acaaagtgat
     6781 tgcccgcccg ggagacggca ttctgcccca cacaactgaa gcattgaccg tgacggttga
     6841 aacaagcaaa acaccagcaa caaccgtcac ctcagagatt atcagttgta atttgtgtct
     6901 tgacccggca tgtcagcggc aaaaaggtga gccccgctca ctttgcctgt acgccaataa
     6961 gcgaggggag tagcagtgga taaacaaacc ttgcaggaaa cagtgagcca gattctggat
     7021 gagatgcgcg agcgccctat cccactgggc gtttccagtc gccatataca tctttcatca
     7081 gcggattatt tgcgtctttt tcctgaacaa ccgattagcg agaaaaagcc gttaatgcaa
     7141 cctggccaat acgctgctga acaggttgtt acgctggagg gaccaaaagg caaattgcac
     7201 aacgttcgct tgttggggcc gctacgcaat gcaagccagg tggaaatttc ccggacggac
     7261 gccagaacgt tagggatcac cgccccgcta cgcatgtcag gcgatctcag cggcactccg
     7321 gggattcgtt tgataagccc atttgcggaa ctggagatca catctggcgt cattgtcgcg
     7381 caacggcata ttcatatgtc tccgctggat gccctgattt atggcgttac gcatggcgat
     7441 aaagtggcgg ttgccattga aggtgatgag cgacgactga tattcgaaaa cgtcgctgtt
     7501 cgcgtctctc cggatatgcg tcttgaaatg catattgata ccgatgaggc caacgccgca
     7561 ggcgctgata gccctgacgt ttttgccaca ctggtgtcag tgccatgaat aacatgctga
     7621 tgcaacgtat tgttgacgaa gtggtttttc gccttaaaca acgtgcggga aaaacactcg
     7681 tgctgactgt ttttcaattg cgagacgcca gcgttcaaga gagcgttcat cagtatgcgt
     7741 cattacaaat ccgatatgtc gatctgccgc tgctgcgaca acttgcggag aacgaaacgt
     7801 cagatcgagc atctatccag atacacgaag cgttagcctg gggaatgcac attcaattgt
     7861 cgttgcagcg tcatttcctg aacgccatcg aattgaagac tctcgcccgt ctaccgctaa
     7921 gctggtgcga cgaacaaggg cagccgatct accttcatca taatcgactt ttaagttatg
     7981 ccgatatcgc acagttaagc tcgggaatac tggtgttaca gcggaaatgt tgtgtgaccg
     8041 cactggctcg tgaagcggca attacccgaa atattcagtt aatcaggcag gagtaaccta
     8101 tgcatctggc tcgagtcact ggcgcggtag tatccacgca aaaatcacct tctctgatcg
     8161 ggaaaaaact gctgctggtt cgccgggtca gtgctgacgg cgaactgcct ctcacgccat
     8221 taaccggcga cgaagttgcc gttgattcag tcggcgcagg cgttggtgag ttggtactcc
     8281 tcagcagtgg ttccagcgcc agacatgtgt tttcaggccc taacgaggcc atcgacctgg
     8341 cagtggtcgg cattgtcgat acgctttcgc gctaaggagc taaaagatgg caatttatac
     8401 ccgaacaggt gattcaggaa gtacgtcttt atttaccgga cagcgcgtca gcaaaactca
     8461 tctacgtgtc gaaacctatg gcacgctgga tgaactgaac gcgacactca gtctgtgtta
     8521 ttgcgccacc gccatcgaaa gccatcgcat cttactggaa gccattcaac aacagatatt
     8581 ttggtttagc gccgaactcg ccagtgaaag cgaacagccg tcagcgcaac agcgttacat
     8641 cggtacggaa gaaattgccg cgttggaaaa cgcgatcgat agtgcaatga acgccgtccc
     8701 cccagttcac agcttcattc tgcctggtcg atgtgaagcc gcaagccgca tgcatttcgc
     8761 ccgcacggtg gctcgacggg ctgaaagacg gctggttgaa ctgacaacgg aaaccaccgt
     8821 ccggaatgtt ttgctgcact atatcaaccg actttccgat tgcctgtatg ccctcgcccg
     8881 cgtggaagac aacgttgctc atcaaaacct gatgattcag gaaatcacaa aacgttatca
     8941 cgaagccaac cacacaccag cattgaagga acgcacaatg ccgctcactt ttcaggatct
     9001 tcaccagctc attcgtagtg cggcaatgcg cgcggatgaa ctgcatattc ccgttgtcat
     9061 cagcattgtt gatgccaacg gaacagaaag tgttacctgg cggatgcctg atgccctgtt
     9121 ggtcagcagt gaactggcac cgaaaaaagc ctggaccgcc gtggcgatga aaacggcaac
     9181 ccacaagctt gctgataccg tgcaacccga cgcgccgctt tacgggctcg aaagccatat
     9241 gcaaggaaaa gtcgtcactt ttggtggcgg cttccccctc tggcgtgacg gaaaattgct
     9301 tggcgggctt ggcatcagcg gcggtagcgt tgaacaagac atggatattg cccaaagcgc
     9361 aatggcggca attaacgtgg gagtaaacca atgaataact ctgaattaga aacactgatc
     9421 cgcaatattc tgcgtgagca acttgtcccc gctacaccac gaacgcaaag aaacgctatt
     9481 ttccagactg tcgatgaagc ggtctgcgca gcacactagg cctttttacg ctttcaacag
     9541 tgcccgctta aaacccgtag cgccattatc agcgccattc gcgaagaatt aatgccccgc
     9601 cttaccgaac tggcagaaga aagcgccaaa gaaacgggga tgggaaataa agaagataag
     9661 tacctaaaaa acaaagcggc actcgataac acgccaggaa ttgaagattt aaccaccaca
     9721 gcactaaccg gtgatggtgg catggttttg tttgagtact ctccgtttgg ggtgattggc
     9781 tcggtcacgc caagcaccaa ccccacggaa accatcatta acaacagcat cagcatgctg
     9841 gcagcaggta atagcgtcta cttcagtcca cacccgggtg ccaaaaacgt ttcgttaaaa
     9901 ctgattacca caatcgaaga catcgcgttt cgctgtagtg gcattcataa cttgatcgtt
     9961 accgtatctg aaccgacctt tgctgccacc gaacagatga tggcgcaccc taaaatcagc
    10021 gttctggcga ttaccggcgg tccggggatc gtcgccatgg gaatgaaaag tggcaagaaa
    10081 gtgattggtg ccggagcggg taaccctccg tgcatcgtcg atgaaacggc ggatattgtg
    10141 aaagccgccg aggacatcat caacggcgct tcatttgatt acaacctgcc gtgtattgct
    10201 gaaaaaagcc tgattgtcgt ggcatcagtg gcagattatt taattcagca gatgcaaagt
    10261 tttggcgcac ttcttcttaa ctatgagcaa actgagaaat tgcgtgcaat ttgcctgcca
    10321 gacggtagcg ccaacaaaaa actggttggg aaaagcccat cagccttgct ggaagcggca
    10381 ggcctccctt tgcccgcaaa agcgccgcgc ctactgattg ctgttgtcga cgccgatgat
    10441 agttgggtta cttgtgagca actaatgcca atgctgccca ttgtgaaggt caaggatttt
    10501 gacagcgctc taaccctggc gctgaaagtg gaagacggct tgcaccacac ggcaattatg
    10561 cactctcaga acgtctcacg actcaacctt gccgcgcgcg ttatgcaaac ctcaatattt
    10621 gtcaaaaacg gcccctcctg ggcgggtatt ggcgtcggcg gcgaaggctt tacgaccttt
    10681 accattgcaa caccgactgg agagggtaca acgtcagcac ggacttttgc ccgctcacga
    10741 cgctgcgtgt taaccagcgg cttttcaatt cgctagagaa acggaaatga atactttttc
    10801 actgcagacg cgtttataca gcggaccagg aagtctcaag gtacttagcc gtttctctca
    10861 tagacacatc tggattattt gcgatgcctt tctggcacgc tcaccactta ttgacacgtt
    10921 gcatcaggcg ctgcccgaca gtaatcgtct cagtattttt agcgaaataa cgcccgcccc
    10981 aactatccag accgtggtga aaggcattgc gcaaatgcag actttacgcc cggatgtagt
    11041 tatcggtttt ggcggcggtt ctgccctgga cgccgcaaaa gcaattgtct ggttcggtcg
    11101 ccagtgtggc attgaaattg aaacctgcgt agccattcct accaccagcg gtatcggttc
    11161 cgaagtgacc agtgcttgtg tcatcagcga tcctgagaaa gggattaaat atcccctttt
    11221 cgataatgca ctttatccgg atatcgcgat ccttgatcca tctttggtcg tcagcgtgcc
    11281 accggcgata acggccaata ccgggatgga tgttttaacc catgcgcttg aagcttatgt
    11341 ttctcctcgc gccagcgact tcacggatgc actggtcgaa aaagccgtgc aaattgtttt
    11401 tcaatacctg cccactgcag tgaaaaaagg cgattgtctt gccacccgtg gcaaaatgca
    11461 taatgcatcg acgcttgcag gaatggcctt tagccaggcc ggactcggtt taaaccatgc
    11521 aattgctcat caactcggcg gacagtttca tctgccgcat ggactggcga atgcgctcct
    11581 gctcacggca gttatccgct ttaatgctgg cgatccgcgc acagccaaac gctatgcccg
    11641 gctggcgaaa acgtgccatt tatgcccgga caacgccaat gacaccgcca gcctgaatgc
    11701 acttattcaa catattgagc agcttaatac aacctgtacg ctgccgactc ttgccaatgc
    11761 gctgaaagaa aaaaaagcag aatggtcaat acgcatacca gatatggttc aggcggcgct
    11821 ggcggatgca acgttgcgta ccaacccgcg tgcagctgat gcctccgcga ttgcagaact
    11881 gctcgaggag ttgttatgaa cgtcggatta atctcctgcg acgaccagac catccgcgag
    11941 cgagtacaag cagcaggtgt cgtgggtgct ggcggcgcag gttttccaac ccatattaag
    12001 ctacaagcgc gtgctgacac tttcctggtg aatgcggcgg aatgcgaacc catgttgaaa
    12061 gtagatcaac agttaatggc gcagcaagcc agccgcctgc ttcgcggtgt tcactatgcg
    12121 atgaaagcca ccggggccag tagcggaatc atcgcgctga aagaaaaata tcaacgggca
    12181 ataaacgcac tcacaccact gttaccaccg gatatccgcc tgcatatttt gcccgacgtt
    12241 taccccgcag gagacgaagt gctgaccatc tggatggcaa ctggtcgccg cgttccaccc
    12301 gctgcattac ccgttagcgt cggtgtggtg gtcaataatg ttcaaaccgt acttaatatt
    12361 gccagagccg tcgagcaaca gtatccagtg acgcgccgca cactcaccgt taatggtgca
    12421 gtggcgaagc caataacggt aaccgtcccc atcggcatgt ctttacgtga agtcctggcg
    12481 ctggcgggcg gcgcaacagt ggatgctcct ggttttatca atggcgggcc gatgatggga
    12541 agtttgatca catcgctcga cacaccagtc agcaaaacca ctggcggctt gctggtttta
    12601 cccaactctc attcactgat ccaacgtcga atgcagaatg accgcagcgt acttgccgtc
    12661 gcgaaaactg tttgtgagca atgccgcttg tgtaccgatc tctgcccacg tcatttaatc
    12721 ggtcacgaac tggcacccca tttattagta cgtgcggtta actaccaaca gctcgccacg
    12781 ccgcaactgt tactgacagc gctcacctgt tcagagtgca acgtctgcgc cagtgtcgcc
    12841 tgtccggtag gaatttcacc aatgcgcata aaccgcctgc ttaagcagga attacgcgca
    12901 caaaatctcc gctacgaagg tgccttaaat cctgcagatc ccatggcaaa ttatcgactg
    12961 attcccgtaa aacggctggt aaccagactt ggcttaaccc cgtggtatca ggatgcgcca
    13021 ttgtctgaac aggtgcctca gcccgaaaaa gtgacgttat tgttacgcca gcacatcggt
    13081 gccagcgcaa ttgcgtgtgt acaaaaaggc gatcgcgtcg ttcatgggca atgtgtaggt
    13141 caaattccac acggcacatt aggtgccccc attcatgcca gtattgatgg catggtcagc
    13201 gatgtgacag aaaacgcgat tacgcttgtg agaggttaac catgtcgaaa gcggtaggta
    13261 ttttagaact tagcagtatc gcgaaaggga tggaaaccgg cgacgccatg ctgaaaagcg
    13321 ccaacgttga gttactggtc agcaaaacgc tatgccccgg aaaatttctg ctgatgctcg
    13381 gtggcgatgt gggcgcggtg caacaggcca tcaacagcgg agcgtccctt gctggtgaaa
    13441 tgctggtcga cagtcttgtc ctgccgaata ttcatcccag cctcctttct gcaatcagcg
    13501 gacttaacca tgttgaacag caccgggctg tcggcgtagt cgaaacctgg agtgtcgccg
    13561 cctgcattga cgctgccgat tgtgctgtca aagctgcaaa tgtcattctt gttcgcgtac
    13621 atatggcttt tggcattggc ggtaaatgct acatggtggt tgctggcgat atttcagatg
    13681 ttaataacgc ggtctctgcc gccagcgtgc gcgcggggga aaaaggtttg ttggtctatc
    13741 ggtcggtgat tgcccgtcca catgaagcca tgtggcgtca gatggtggag ggataatgac
    13801 aactcagcac acaactgaac ggatgattca ggaatatgtc ccaggtaagc aggtcacgct
    13861 ggcacatgtc atcgccaatc cagggaagga tctgtttaag aaattgggtt taccagacac
    13921 catttccgct attggtattc tgacaattac ccccagcgaa gcatctatca tcgcatgcga
    13981 tatcgccaca aaatcaggcg ctgtagagat cggttttctt gatcgtttta ccggtgccgt
    14041 agtgctgact ggtgatgttt ctgcgatcga atatgcactt agccaggtta cccgtacgct
    14101 gggtgaaatg atgcgcttta ccgcctgccc cattacgcgg acctgacgtt atgcaacgta
    14161 tgatgctcat cggccccagc cagtgcggaa aaacgtcact cacgcaatgt ctgcgtggcg
    14221 aaaatattca ttatcagaaa acgcaggcaa tcgtctggtc cccgaccacc attgatacac
    14281 caggcgaata cctcgaaaac cgcagcctct acagtgcact gttggtcagt gcctgcgaag
    14341 cggacattat cgcgctggtg cttaatgcgg acgcaccgtg gtcacctttt tcgccaggct
    14401 ttacctcgtc aatgaatcgc ccggtcattg gagtagtaac caaagcagat ttagccaaca
    14461 tggaacaaat atctttggtg aaatgttggt tacgggaagc tggagcgcac aacgtgttag
    14521 taaccagtgc ggttaataac catggagttg cagaactttt agccttgctg catacagaag
    14581 aaggctgtcg ctaatttcga caggttatca gatgggaatg atgacggtga tacacagaaa
    14641 agaatcaatg agcaatgagg gttgccgggc aaccctcatt gaataaaaca ggaattactt
    14701 cgcttcgccg tttttcattt cgcccatagc tttcagcttt ttggagatat cgcggcgttc
    14761 tttggagagc tcggcatttt tgatgatgta atcgtcaacg cgatcttcat agtcaccttt
    14821 catgctggcg atgatgccct ggattgcttc aacgctcatc ccaggcttga tgtaatcgct
    14881 caggttgtcc agcagcagga cgcgcttctg gttgtcacgg atctttttct caacgtcctg
    14941 aatttcacgt tgcagtttgt tcttacgacg gaacagacga acaaattcca gtacgtcctg
    15001 gaatgaaggc ttggtagttt ccatttttat acccctgata atgtgagagt cagattcgtt
    15061 taagcaaccg ctattcgtta gggccaacat tactcatagc tgccgcagat gacaatgctt
    15121 ttatcccttt tcactatcat accctttatc cttgctgaat cgaagcagca gcaagatgat
    15181 tctgaagttc aggaattatt tgcgcaaggc ccggcaaatc agattcgata gcaactcaag
    15241 ctgatgcgcc gtttccatgt tcagccacac ataaccatag attggtgttt ccactacctt
    15301 caggtcatgg tggttattga gcaactgacg cagctcttcc acagcatcgt tcaatttttc
    15361 cgtattggca aaaaccggct gcggattacc ttcgtacagc gcatgaacaa agctcagcag
    15421 tatttgctgc atcatgtgct gggtatcacg aagtttttgc gcgttcaata acacgaaatg
    15481 gctggggcgc gtggcccagt acgcattgat ttgcaactcc agcatacaaa ccagattgcg
    15541 gttaatggtc tggatacctt catatatcga ttttggaata cgggtttctt tgctggcggg
    15601 cgcaatcagc ccacgcattt tcacggcatc ggtcaggagt ttttgtagat ggctttccag
    15661 acgtgggcgt tcgagtaagt tcggtgagaa tgcagattga tagacccgat tatactcggt
    15721 cagacttttc gccagttgaa tgcgccagtg gatgaacgcc cgttgtggcc agataccggt
    15781 aaacaacatt gccagtaaag agccgaggat cacatcgccg cttcgccata acgccgtatc
    15841 aatttcacct gtcggggaac caacaacaat tgccagcgtc accccaatca ataatccttg
    15901 atacggtttc ttgcccagcg ccagccaacc gcaaagaaac atcgccgccg cgcaccagac
    15961 taacatcagc ggtaacgaaa ttaactccag ttgcagagcg ataagaccta aaatcgaacc
    16021 caacaccgta ccgccaatac gttcgaaggc acgagggaca acgttacccc agaacgagat
    16081 tggccccata atcaccacca tggtgaccag cggccaggtg ctttccggga tagtaaacag
    16141 gcggataatg agaaaagtga gcaggaatgc cagcgcgacc cgagtaccat gcactatgcg
    16201 gtaatggcgg tatacccgga tttcaaacgg gcttaatgac ttatcggcac gcacgcagaa
    16261 actccgtaat gaagattaaa agcgctcata actcatctgt cggccagaag gcgcgcctct
    16321 gaattatccg acggacaaac cgggaacagt gtaatttccc ggcaggagcg aaaaaattga
    16381 aaggcgcatc ttactctttt cgctttcaaa aaaagagtgg tcatcgcgtt aacacaccgc
    16441 cctgagatga attagtgatg ttttggctgc accgcaacat acatttcgat atcccagtac
    16501 ccatcttccg cgccattgtt caaataaacc tcgaagcagg gctttggtaa catttcataa
    16561 gcactgtcct gcaagaggct attaaagaac tgataccagg gtttagcaaa atcatcaccg
    16621 actacacgag ccaccgccac cgcatactga ccacctgaaa tttctgtcag aatgacgccc
    16681 tcactgtttt cgggaagcgt aaagttattc ggtaccgtca caacggtgtc gcagcgtaat
    16741 ttttcggcgg gtgtttcatc tggattgtcg taatagacag caacccactc cttcggcaca
    16801 atatttttgt tatctaccca catcatcaac tgctcaaagc ctttctttac cgtctgttcc
    16861 cacgggccaa cgagatggaa acctgcaacg gtacgtttct cttcctgcgt aatctcgtag
    16921 ttcatgacgc ctccattaac actgttttta tatacagcat agttgcaata ttaaaaccac
    16981 aaggaatgag tgttgattat gcgagcagac tcgcactcct gccagtctgc tgcaaaagaa
    17041 aggtcaggcc ttatggtgga aataatcact caggcgggaa aacatgccgc cttccccgac
    17101 agattccagg gtaaccagcg gccagtgcgc cacctgttta tcacggtcgt aaagttcaat
    17161 ttcccctacc cgctgatggg cgctaattgg cgcggtgagc tctttaccat caagggtata
    17221 tttggctttg atatgtggaa tttcggcttt cggtagcacc atccagaact cttgttccgt
    17281 tcctaggtcg atattttctt tatcgccata ccagatgcgt tccgttccga cctttttccc
    17341 acggtgcaaa atttgcaccg tagtaaagtt ttgttgcccc caacgcagta attttcttgc
    17401 ctcttcctca cgaccttttg cactgtcagc ccccattacc actgcaatga gacgacgctg
    17461 cccatctaca gccgaagcaa tgagattaaa cccggcacca gaagtatgac ccgttttcag
    17521 gccgtcaaca ttcatggttt tatcccacaa caacccgtta cggttttgct gggtgatacc
    17581 gttccaggtg agacttttct cactgtacat atgataaaac tcgggctcgc cgtggatgat
    17641 agcgcgagaa agcacagcta aatcataagc cgagctatgc tggccaggtg catccagacc
    17701 atgcactgtt tcaaaatgcg tatccttgag atgcagcttc tcggcatagt tgttcatcat
    17761 ttcaacaaac tgccgttgcc caccggcaat atagtcagcc agagcaacac aagcgtcatt
    17821 tccggaatcc acaattaaac cacggcttaa atcacgtacc gatacgcgat cgccctcttt
    17881 caaaaacatc agtgaagaac cgacaaacac cggattatct ttcgcccacg catcgcgccc
    17941 cacggtgaca atatcgtctg gcgtaatgcg atgactatcg atagcgcgat ccacgacata
    18001 acccgtcatc agctttgtca ggctggcggg attgcgctgt tgatgctcat tacccgcggt
    18061 gaggatctga ccggtggtgt aatccatcaa tacccaggac ccggcatgaa tctctggagg
    18121 ctgaggtgaa aaaggaatgt tttccgccgc aaaaccagac gataagttaa aaacgaacaa
    18181 agaagcagca ataataagac ggcgtttcaa cagcaaaccc tcaggagttt caaatagctg
    18241 ttctttttac ggaaatactt atgaactggc tggaataaag tgcaagaaaa tgtgactacc
    18301 ctctcatttt tatctgacat gatctgttgc cactcgctgc caaattgtgg cgctaaagct
    18361 gattagcacg gtgatatttg atactctggc agacagcaga aataacggat ttaacctaat
    18421 gatgaatgac ggtaagcaac aatctacctt tttgtttcac gattacgaaa cctttggcac
    18481 gcaccccgcg ttagatcgcc ctgcacagtt cgcagccatt cgcaccgata gcgaattcaa
    18541 tgtcatcggc gaacccgaag tcttttactg caagcccgct gatgactatt taccccagcc
    18601 aggagccgta ttaattaccg gtattacccc gcaggaagca cgggcgaaag gagaaaacga
    18661 agccgcgttt gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg
    18721 ctacaacaat gtgcgtttcg acgacgaagt cacacgcaac gttttttatc gtaatttcta
    18781 cgatccttac gcctggagct ggcagcatga taactcgcgc tgggatttgc tggatgttat
    18841 gcgtgcctgt tatgccctgc gcccggaagg aataaactgg cctgaaaatg atgacggtct
    18901 accgagcttt cgccttgagc atttaaccaa agcgaatgga attgaacata gcaacgccca
    18961 cgatgcgatg gctgatgtgt acgccactat tgcgatggcg aaactggtaa aaacgcgtca
    19021 gccacgtctg tttgattatc tctttaccca tcgtaataaa cacaaactga tggcgttgat
    19081 tgatgttccg cagatgaaac ccctggttca cgtttccgga atgtttgggg catggcgcgg
    19141 caataccagc tgggtggcac cgctggcgtg gcatccagaa aatcgcaatg ccgtaattat
    19201 ggtggatttg gcaggagaca tttcgccatt actggaactg gatagcgaca cattgcgcga
    19261 gcgtttatat actgcaaaag ccgatcttgg cgataacgcc gccgttccgg ttaagctggt
    19321 gcatatcaat aaatgtccgg tgctggccca ggcgaatacg ctacgcccgg aagatgccga
    19381 ccgactggga attaatcgtc agcattgcct cgataacctg aaaattctgc gtgaaaatcc
    19441 gcaagtgcgc gaaaaagtgg tggcgatatt cgcggaagcc gaaccgttta cgccttcaga
    19501 taacgtggat gcacagcttt ataacggctt tttcagtgac gcagatcgtg cagcaatgaa
    19561 aattgtgctg gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgtcgataa
    19621 acggattgaa aagctgttgt tcaattatcg ggcacgcaac ttcccgggga cgctggatta
    19681 tgccgagcag caacgctggc tggagcaccg ccgccaggtc ttcacgcccg agtttttaca
    19741 aggttatgca gatgaattgc agatgctggc acaacaatat gccgataaca aagagaaagt
    19801 ggcgctgtta aaagcgcttt ggcagtacgc ggaagagatc gtctaatttg aagcctccgc
    19861 cgctggtacg gcggaggtca atgttgagtc aggctttttg aacggtgata ctccacgctg
    19921 catcgccaat ctgctgataa tcggtgatgg catgcccttc ttctgccgcc cactgtggga
    19981 ttgcttcagt tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt
    20041 ctgccagtgc tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca
    20101 gctttttaat cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgcgcc
    20161 ttacgctgcg gtcgaacata cacaagccag gacgcagtcc agactccgag aatcataaat
    20221 acaaggccaa tccagccctg ccaggtcatc attgcagtca taaccagtcc attaccgata
    20281 aagcaaccac ctgcaatact ggcaccgaag cccatcagta caccacctaa cccgctacgt
    20341 aatgttgttt gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata
    20401 aatgacccca cgaagatccc taacaccagg aaaacgcccc agttaatgta tttcacgtca
    20461 cccgcgacca gaaattgcag aatattggcc gttggggaag tgattcctaa cccaaacatg
    20521 cgtccggttg cttcactcag gggccaggct aaaagcgcaa tcaaaccgat gagtacagca
    20581 gtgacaaagg gatgccagcg tttttcaaac agaatatgag cgatcccagt tcggcgcggc
    20641 ggtaaggtcg cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt
    20701 atcaccagca aaacggcaac caacggccaa acagacaaat taaaagtctc agcaatagag
    20761 ttatgttcag tactgtagtg ctgcaaggtt tgatttaaac cactggcatg tggagaacgc
    20821 atcaccgcac tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca
    20881 cccgcgcgat accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaag
    20941 atatacccac ctataacagt accaagccac gggaacgcct cggcttcgta agtcagtagg
    21001 cccgcctgaa ttaacgcaaa aacccccacg ctttgtaccg aaattgcaat cagcaaggcg
    21061 taaaacatgc gattattttt cgcgatatac atatcgcgaa aaccacctgt caggcaaaaa
    21121 cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt
    21181 gaaaacataa attaattaac cagatgaatg ttaatgagga aattattcat gactggtgga
    21241 atgcagacca ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga
    21301 taaaaaaaaa taaaaaaacc ggagccaaat attcattcga ctccggttga ttattagaaa
    21361 agatgattat gcaacgtctt cgtactgcgg aactgggtta cggaagctct tggtgacgca
    21421 agccaggtaa atcagaccga tagcagccca aatcagaccc agaaccattg agctttcttc
    21481 caggttaacc cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg
    21541 gaagtgatct ttcagcgtct tattacgctt ctcacggatc cagaactgcg agatgaccga
    21601 caggttaacg aaggtgaacg ccaccaacgc accaaagtta atcagcgctg tagccattac
    21661 caggtcgaag ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg
    21721 agtacgccat ttcgggtgta cataaccgaa gaagcttttc gggaatacgc cgtcacgacc
    21781 cattacgtac atcagacgcg ctacgcctgc atgcgccgcc ataccggacg ccagtacggt
    21841 aatggtggag aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat
    21901 ttcaggctgt gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag
    21961 gaagtaagtt gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg
    22021 cgggatcacg cgctctgcat ctttggtttc ttccgacagg ttgctgatgc cgtcaaagcc
    22081 ggtaaaggaa aagcacagga ttgtcgcccc ggtaatcatc ggaataacat gcgcatcgcc
    22141 agaccagaac ggacgagtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata
    22201 aacaaccatg cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt
    22261 gaagttcgct acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc
    22321 caccacaaac atccacgatg ggatggaagg caccagagct tcaaaataga ttttcgccag
    22381 cagaatgttg atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa
    22441 gccgacagtc gggctaatgg atttctgggc gtaagtatat gcagagccag cagaaggata
    22501 gcggcgaacc agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc
    22561 ataggctgtc ggcacatgac catccgtgag gcctgaaacg ataccaaaag tatcaaacag
    22621 cgtcatcggc tgcatatagg caagacccat cattacaacc ggaactaacg taagcgtttt
    22681 acgcaattcc acgcgagagg tgtttggagt aacgttatgc gacatggtca ttctccttta
    22741 cggtgataac cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa
    22801 caactgtcga tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg
    22861 attggtttga agcaaaaaat aaccgacgct gatgaaacgt cggtttttag tcattttttg
    22921 acagcggcgc attgtgccta aatggggggg aaatgacaag agaatgagaa gcttgtcata
    22981 ataatttttc tttaaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc
    23041 gcactatttg ctaaaatttc atcccgccac cagccgctgg caagccctgc ggtctgttcg
    23101 ttccagccaa tccagacggg ttcaaagaga gtttgggcga ccactttctt ctcgattaac
    23161 gcgccactat cgagaaaacg ttgcaccaga taacggggta aatagccgca gccgaggccg
    23221 ctaatttgca gctccagctt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg
    23281 agaagttgtg aggccgtaga agcgcctgcc tgcgcagtgt cccccaccac aatcgcccga
    23341 tatcgcttaa tgatacgacg gtttaacggc tcttcttcct gggctaacgg atgatgaggc
    23401 gcgaccgcaa agacttgttc cagatcgccc aaccgcgaga aaccaaactc actggaagat
    23461 ggtggctcat gcatcgcgcc aacaataata tccgctcgcc cctgagtcag ggcatcccag
    23521 gaaccaccga gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa
    23581 gcttcaatca gtggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc
    23641 tcgttttccc agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc
    23701 agaacttccc gccccttctc taataacatt tttccggtac gggtgaattt agcgcggtga
    23761 ccgctacgat caagtaattg aatattaagg tcgctttcca gcttgtgaac ggtgtaactc
    23821 agagcggacg gcgtcttgta gagtttagcc gacgccgccg caaaactgcc ttctttttct
    23881 aaggcatcga ggatcatcaa cacatccagc agttgtttca tacacgcccc cttgcagtgc
    23941 atgcggcgtc ctgctggcgt gatcgtttac tccagcggca ttaccagcgg atcgggatac
    24001 tggtattcaa atcccagttc attacaaatc cgactgccat caataatctt acctttgccg
    24061 ctgtccagac tatttctgaa ctgcggaggt tccagcccca gtaaacgggc catctgcgga
    24121 tagaaaacat tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta
    24181 ggtgcctgta acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca
    24241 ccatgttcac catcaggcgc ggtttttccg gcaaagaagc gtccgggatg acgtcccggt
    24301 ccgaccagcc ccgcaagacg cagaatatcg accgaagtac cgggtaaatt gtgcagccag
    24361 tcttcgagtt cttctaatac tcgtccactg ttggtcaccg gattacgcgg ggtggtttct
    24421 ttcaccgtgc cttgcgcgtc gccatagaca gacgttgagc tggtaaaaat aatgcgagga
    24481 atacgatggg ccagcgcgct atccactaac tcttgtaccg cttgtaaata gaactcatcg
    24541 ccggggccgc tacgacgtgc cggaagcgta atgaccagcg catcggcatc catcagggca
    24601 tccagatcgt cagaatcgca aactaactca ggctccatgc gaagcagata gctatcaatg
    24661 ccactcattc gggccgcttc gacaccatct tgtgtggttt tactcccggt gacttgccag
    24721 cctcgcgctg aaagtgacat cgccagcggc atgcctaacc accctaaccc gacaattgcg
    24781 acctttttca tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc
    24841 aaaactgaca attcattcta tgaatgaatc tgttcaataa tgataacgac atgctgcaat
    24901 gagcagtgaa tcgtcggtaa ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga
    24961 ccagaaacct gacaaattat gtttcagggg ttctggcttc cccttacctt caaatggcgt
    25021 tctgcgggta tctttgataa gttcattgat ctttttaaca attcgcttat ctgtttcctg
    25081 ccagtacaga taatcatccc atgattcctc agaccagatt agtttcactc aataatgtcc
    25141 ttttctgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct ccgggcgtta
    25201 gcgggggagc gcagtagata agccgtctct tccagcgagt tgtattcttc gagtgacatc
    25261 agaacacaag cctctccatt ctgacgagta ataaggatcg gggcatgatc ttcaacggct
    25321 ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt acgcatgtca
    25381 atctcctctt ttgtacagtt tattgtacaa tgatgggcgt taattaacta tttattaatt
    25441 agtttgtaga tcaaggtatt gtcagtgaga cgaaaatcca ggcttcgcta tttttggtgc
    25501 cgtcagctta gaggacagtc ctcttagccc cctcctttcc ccgctcattc attaaacaaa
    25561 tccattgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt ggtttaggtt
    25621 aaaagacatc agttgaataa acattcacag agacttttat gacacgcgtt caatttaaac
    25681 accaccatca tcaccatcat cctgactagt ctttcaggcg atgtgtgctg gaagacattc
    25741 agatcttcca gtggtgcatg aacgcatgag aaagcccccg gaagatcacc ttccgggggc
    25801 ttttttattg cgcggttgat aacggttcag acaggtttaa agaggaataa caaaatgaca
    25861 gacaacactc gtttacgcat agctatgcag aaatccggcc gtttaagtga tgactcacgc
    25921 gaattactgg cgcgctgtgg cattaaaatt aatcttcaca cccagcgcct gatcgcgatg
    25981 gcagaaaaca tgccgattga tattctgcgc gtgcgtgacg acgacattcc cggtctggta
    26041 atggatggcg tggtagacct tgggattatc ggcgaaaacg tgctggaaga agagctgctt
    26101 aaccgccgcg cccagggtga agatccgcgc tactttacct tgcgtcgtct ggatttcggc
    26161 ggctgccgcc tttcgctggc aacgccggtt gatgaagcct gggacggccc gctctcctta
    26221 aacggtaaac gtatcgccac ctcttatcct cacctgctca agcgttatct cgaccagaaa
    26281 ggcatctctt ttaaatcctg cttactgaac ggttctgttg aagtcgcccc gcgtgccgga
    26341 ctggcggatg cgatttgcga tctggtttcc accggtgcca cgctggaagc aaacggcctg
    26401 cgcgaagtcg aagttatcta ccgctcgaaa gcctgcctta tccagcgcga tggtgaaatg
    26461 gaagaatcca aacagcagct gatcgacaaa ctgctgaccc gtattcaggg tgtgatccag
    26521 gcgcgcgaat caaaatacat catgatgcac gcaccgaccg aacgtctgga tgaagtcatc
    26581 gccctgctgc caggtgccga acgcccaacc attctgccgc tggcgggtga ccaacaacgc
    26641 gtagcgatgc acatggtcag tagcgaaacc ctgttctggg aaacgatgga aaaactgaaa
    26701 gcgctgggtg ccagttcaat tctggtcctg ccgattgaga agatgatgga gtgatcgcca
    26761 tgagctttaa cacaatcatt gactggaata gctgtactgc ggagcaacaa cgccagctgt
    26821 taatgcgccc ggcgatctcc gcttctgaaa gcattacccg cactgttaac gatattctcg
    26881 ataacgtgaa agcacgcggt gatgacgccc tgcgggaata cagcgcgaag tttgataaaa
    26941 ccacggttaa cgcgctgaag gtgtctgctg aggagattgc cgccgctagc gaacgcctga
    27001 gcgacgagct aaaacaggcg atggcggtgg cagtaaaaaa tattgaaacc ttccacactg
    27061 cgcaaaaact gccgccggta gatgtagaaa cgcagccagg cgtgcgttgc cagcaggtca
    27121 cgcgcccggt cgcttcagtt gggttgtata ttcctggcgg ctccgccccg ctcttctcaa
    27181 cggtattaat gctggcgact ccggcgcgta ttgcgggctg taaaaaagtg gtgctgtgct
    27241 caccgccgcc gattgccgat gagatccttt atgcggcgca gctgtgcggt gtgaaggacg
    27301 tgtttaacgt cggcggcgca caggccattg ccgcgctggc gtttggtacg gaatctgtgc
    27361 cgaaagtgga caaaatcttc gggccgggta acgcctttgt caccgaagcg aaacgtcagg
    27421 tgagccagcg tctggacggt gcggctatcg atatgcccgc aggcccgtcg gaagtgttgg
    27481 tcattgccga cagcggcgca acgccggatt tcgtggcttc tgatttgctt tctcaggctg
    27541 aacacggccc ggactcacag gtgattttac tgacgcctga cgctgatatg gcgcatcaag
    27601 ttgccgaagc cgtcgaacgc cagttagcag aactgccgcg tgccgaaacc gcccgccagg
    27661 cactgaacgc cagccgcctg atcgtgacta aagatttagc gcagtgcgta gagatctcca
    27721 accagtacgg cccggagcac ctgatcatcc agacccgcaa cgcccgcgat ctggtcgatg
    27781 gcatcaccag cgccggttcg gtatttcttg gtgactggtc accggaatcg gcaggtgatt
    27841 acgcctccgg caccaaccac gttctgccga cttacggtta caccgccacc tgttccagcc
    27901 tcgggctggc agatttccag aagcgtatga ccgtgcagga actgtcgaaa gaaggtttct
    27961 ccgcgctggc ttcgaccatt gaaacattgg ccgccgccga gcgcctgacc gcccataaaa
    28021 atgccgttac tttgcgtgtt aacgccctta aggagcaagc atgagcaccg tgactattac
    28081 cgatttagcg cgtgaaaacg tccgcaacct gacgccgtat cagtcggcgc gtcgtctggg
    28141 cggtaatggc gacgtctggc tgaacgccaa cgaatacccc actgccgtgg agtttcagct
    28201 tactcagcaa acgctcaacc gctacccgga atgtcagccg aaagcggtga ttgaaaatta
    28261 cgcgcaatat gcaggcgtaa aaccggaaca ggtgctggtc agtcgtggcg cggacgaagg
    28321 tattgaactg ctgattcgcg ctttttgcga accgggtaaa gacgccatcc tctactgccc
    28381 gccaacgtac ggcatgtaca gcgtcagcgc cgaaacgatt ggcgtcgagt gccgcacagt
    28441 gccgacgcag gacaactggc aactggactt acagggcatt tccgacaagc tggacggcgt
    28501 aaaagtggtc tatgtttgca gccccaacaa cccgacaggg caactgatca atccgcagga
    28561 ttttcgcact ctgctggagc taacccgcgg aaaagccatt gtggttgccg atgaagccta
    28621 tatcgagttt tgcccgcagg catcgctggc tggctggctg acggaatatc cgcacctggc
    28681 tattttgcgc acactgtcga aagcttttgc tctggcgggc cttcgttgcg gatttacgct
    28741 ggcaaacgaa gaagtcatca acctgctgat gaaagtgatc gccccctacc cgctctcgac
    28801 gccggttgcc gacattgcgg cccaggcgtt aagcccgcag ggaatcgtcg ctatgcgcga
    28861 acgagtgacg caaattattg cagaacgcga atacctgatt gccgcactga aagagatccc
    28921 ctgcgtagag caggttttcg actccgcaac caactacatt ctggcgcgct ttaaagcctc
    28981 cagcgcagtg tttaaatctt tgtgggatca gggcattatc ttacgtgatc agaataaaca
    29041 accctcttta agcggctgcc tgcgaattac cgtcggaacc cgtgaagaaa gccagcgcgt
    29101 cattgacgcc ttacgtgcgg agcaagtttg atgagtcaga agtatctttt tatcgatcgc
    29161 gatggaaccc tgattagcga accgccgagt gattttcagg tggaccgttt tgacaaactc
    29221 gcctttgaac caggcgtgat cccggaactg ctgaagctgc aaaaagcggg ctacaaactg
    29281 gtaatgatca ccaatcagga tggtctggga acacaaagtt tcccgcaggc ggatttcgat
    29341 ggcccgcaca acctgatgat gcagatcttc acctcgcaag gcgtacagtt tgatgaagtg
    29401 ctgatttgtc cgcacctgcc cgccgatgag tgcgactgcc gtaagccgaa agtaaaactg
    29461 gtggaacgtt atctggctga gcaagcgatg gatcgcgcta acagttatgt gattggcgat
    29521 cgcgcgaccg acattcaact ggcggaaaac atgggcatta atggtttacg ctacgaccgc
    29581 gaaatcctga actggccaat gattggcgag cagctcacta aacgagaccg ttacgctcac
    29641 gtagtgcgca acaccaaaga gacacagatt gacgttcagg tgtggctgga tcgcgaaggt
    29701 ggcagcaaga ttaacaccgg tgttggcttc tttgatcaca tgctggatca gatcgccacc
    29761 cacggtggtt tccgtatgga aatcaacgtc aaaggcgacc tctatatcga cgatcaccac
    29821 accgtcgaag ataccggcct ggcgctgggc gaagcgttaa aaatggctct tggcgacaaa
    29881 cgcggtattt gccgctttgg ttttgtgcta ccgatggacg aatgccttgc ccgctgcgcg
    29941 ctggatatct ctggtcgccc gcacctggaa tacaaagccg agtttaccta ccagcgcgtg
    30001 ggcgatctca gcaccgagat gatcgagcac ttcttccgtt cgctctcata caccatgggc
    30061 gtgacgctac acctgaaaac caaaggtaaa aacgatcacc accgtgtaga gagcctgttc
    30121 aaagcctttg gtcgcaccct gcgccaagcc atccgcgtgg aaggcgacac cctgccctcg
    30181 tcgaaaggag tgctgtaatg aacgtggtga tccttgatac cggctgcgcc aatctgaact
    30241 cggtgaagtc agccattgcg cgccacggtt atgaaccaaa agtcagccgt gacccggacg
    30301 tggtgttgct ggccgataaa ctatttttac ccggcgttgg cactgcgcaa gcagcgatgg
    30361 atcaggtgcg tgagcgcgag ctgtttgatc tcatcaaagc ctgtacccaa ccggtactgg
    30421 gcatctgctt agggatgcaa ctgctgggtc ggcgcagcga agagagcaac ggcgtcgact
    30481 tgctgggcat cattgacgaa gacgtgccga aaatgaccga ctttggtctg ccgctgccgc
    30541 atatgggctg gaatcgcgtt tacccgcagg caggcaaccg cctgtttcag gggattgaag
    30601 acggcgcgta cttttacttt gttcacagtt acgcaatgcc ggtcaatccg tggaccattg
    30661 cccaatgtaa ttacggcgaa ccgttcaccg cggcggtaca aaaagataac ttctacggcg
    30721 tgcagttcca cccggagcgt tctggtgccg ctggcgctaa gttgctgaaa aacttcctgg
    30781 agatgtgatg attattccgg cattagattt aatcgacggc accgtggtgc gtctccatca
    30841 gggcgattac ggcaaacaac gcgattacgg taacgacccg ctgccgcgct tacaggatta
    30901 cgccgcgcag ggtgccgaag tgctgcatct ggtggatctg accggggcaa aagatccggc
    30961 aaaacgtcaa atcccgctga ttaaaaccct ggtcgcgggc gttaacgttc cggtgcaggt
    31021 tggcggcggt gtgcgtagcg aagaagacgt ggcggcgtta ctggaagcgg gcgttgcgcg
    31081 cgtggtggtt ggctccaccg cggtgaaatc accagaaagg gtgaaaggct ggtttgaacg
    31141 tttcggtgcc gatgccttag tgctggcgct ggatgtccgt attgacgagc aaggcaacaa
    31201 gcaggtggcg gtcagcggct ggcaagagaa ctcgggcgtt tcactggaac aactggtgga
    31261 aacctatctg cctgtcggcc tgaaacatgt gctgtgtacc gatatctcgc gcgacggcac
    31321 gctggcaggc tctaacgtct ctttatatga agaagtgtgc gccagatacc cgcaggtggc
    31381 atttcagtcc tccggcggta ttggcgacat taacgatgtt gctgccctgc gaggcactgg
    31441 cgtgcgcggc gtaatagttg gtcgtgcatt actggaaggt aaattcaccg tgaaggaggc
    31501 catcgcatgc tggcaaaacg cataatccca tgtctcgacg ttcgtgatgg tcaggtggtg
    31561 aaaggcgtac agtttcgcaa ccatgaaatt attggcgata ttgtaccgct ggcaaaacgc
    31621 tacgccgaag aaggtgcaga cgaactggtg ttctacgata tcaccgcttc cagcgatggc
    31681 cgtgtggtag ataaaagctg ggtatctcgc gtggcggaag tgatcgacat tccgttctgc
    31741 gtggcgggtg ggattaagtc tctggaagat gccgcgaaaa ttctttcctt tggcgcggat
    31801 aaaatttcca tcaactcccc tgcgctggca gacccaacct taattactcg cctggcggat
    31861 cgctttggcg tgcaatgcat tgtggtcggc attgatacct ggtacgacgg cgaaaccggt
    31921 aaatatcatg tgaatcaata taccggcgat gaaagccgca cccgcgtcac tcagtgggaa
    31981 acactcgact gggtacagga agtgcaaaaa cgtggagccg gagaaatcgt cctcaacatg
    32041 atgaatcagg acggcgtgcg taacggttac gatctcgaac aactgaaaaa agtgcgtgaa
    32101 gtttgccacg tcccgctaat tgcctccggt ggcgcgggca ccatggaaca cttcctcgaa
    32161 gccttccgcg atgccgacgt tgacggcgcg ctggctgctt ccgtattcca caaacaaata
    32221 atcaatattg gtgaattaaa agcgtacctg gcaacacagg gcgtggagat caggatatgt
    32281 taacagaaca acaacgccgc gaactggact gggaaaaaac cgacggacta atgccggtaa
    32341 ttgtgcaaca cgcggtatcc ggcgaagtgt taatgctggg ctatatgaac ccggaagcct
    32401 tagacaaaac catcgaaagc ggcaaagtca ccttcttctc gcgcactaaa cagcgactgt
    32461 ggaccaaagg cgaaacgtcg ggcaatttcc tcaacgtagt aaatattgcg ccggactgcg
    32521 acaacgacac gctactggta ctggcgaatc ctatcggacc aacctgccac aaaggcacca
    32581 gcagctgctt cggcgacacc gctcaccaat ggctgttcct gtatcaactg gaacaactgc
    32641 tcgccgaacg caaatctgcc gacccggaaa cctcctatac cgccaaactg tatgccagcg
    32701 gcaccaaacg cattgcgcag aaagtgggcg aagaaggcgt agaaaccgca ctggcagcga
    32761 cggtacatga ccgctttgag ctgaccaacg aggcatcgga cttgatgtat cacctgctgg
    32821 tgctgttgca ggatcaggat ctggatttaa cgacggtaat tgagaacctg cgtaaacggc
    32881 atcagtgagt tgcagggtaa gcggatgcga tgttgttgtc gcatccggca aaaaaaaagg
    32941 gcaaggtgtc accaccctgc ccgttttctt taaagctgca aagattactt cgcgttataa
    33001 ttacgcagag cgttacgccc cagcacaatc cccgcgccaa ccatgccgcc cagcagcacc
    33061 gccagaatca aggtaattgc ctttttcggg ctatcgcgac gaataggtaa cgtcggtttc
    33121 atcacataac ggtaagcatg aatatcaaga tcatcaacct ttaagttttc aatatccaac
    33181 aggttttgac gtgtctgata gtagtttggt gagaacacca acggacgggt cgcctcatgc
    33241 ttaatcatcg actccagcgc ttcgctcccc aaaaggaaca acgtatcttg tgtcacatct
    33301 tcagtctgct gaacctgtgg ctttgccacc tgcgcctgat tcgcatactg caacgcttcc
    33361 tgaatctgac ggatacgcag atctttctgc tcctgcgcga ctacttcctg cgttcttaaa
    33421 gagtcctgca agtttttccg tcccagagca atgttgtctt tgagatcctt ttctagctct
    33481 tgattcactt tatcatcaac ttgctgaatg tattgggcca acttcatttg tgctccctct
    33541 gcagtttgcc caacataaga aacagtcaat ggtaattgct ggttcttaac agaaggttcg
    33601 atggtaagtt tttctggttc ttcctgatta tccagcgttt ctgctaatgc agagaaggca
    33661 gaactgaagc gaccaattaa cgtctcctgt aaatccgata ctttcggtgc agcctgacca
    33721 tagataacat tcatggcatt gttatagcca gcaatttgcc ccacgtcggg ctgagtgata
    33781 attgctgttg acgtccattt ctccttcgct actgccaaat atccaatagc cagggcaata
    33841 gccacaatga cggaaatgat gattgtcatc ttgccacgcc acaactgcac tagtaaatca
    33901 atcaaatcaa tctgttccgg gtcatggttt tgcccagaaa cattattatt ttctactctc
    33961 atcattaccc taactgacgg aaatttccaa gtccgcatag tttagcgata atcctagtga
    34021 atgctatagg ataagtgata aaaataatga attattgggg aggtaacaaa agacaatcag
    34081 ggcgtaaata gccctgataa caagatgtta gtcgctgcca aagagatcgc gggtgtatac
    34141 cttatctgcc acatctttaa gctcttctgc cattcggtta gagataatga cgtcggcttg
    34201 ttgtttgaag atggcgagat cacgttccag ccgagagttg aagaatgagt cttctttcat
    34261 cactggctcg tagatgatca cttcaacgcc tttcgccttg atacgcttca taatcccctg
    34321 aatagaagac gcacggaagt tatctgaacc gctcttcata atcagacgat aaatacccac
    34381 cacttgcggt ttgcgtgaca aaatggcatc ggcaataaaa tccttacgcg tgcggtttgc
    34441 atcgacaatt gccgagatca ggttattcgg cacagactga tagttcgcca gtaactgctt
    34501 ggtatctttc ggcaagcaat aaccaccata accaaacgac gggttgttgt aatggttgcc
    34561 aatacgcgga tcgagacaaa cgccttcgat aatctggcga gtattcagac ctaaactttc
    34621 tgcgtagcta tccagttcgt taaagtatgc cacgcgcatc gctaggtagg tgtttgcaaa
    34681 aagtttaatc gcttctgctt cggtcgaatc ggtgaacagg gtcgggatat tttgcttaat
    34741 cgcgccttcc tgtaacaacg cagcaaaacg ttctgcgcgt tctgaacgct caccgatgac
    34801 aatacgtgaa ggatggagat tatcgtaaag ggctttacct tcacggagaa attccggaga
    34861 gaaaataata ttttcagtac gatatttctt atgcatcgct tcggtaaaac caacgggaac
    34921 cgttgatttg atgaccataa ccgcataagg atttatctca actacgtctt taattactga
    34981 ttctacactg gatgtattga aataattagt tttaggatca tagtcggttg gagtggcgat
    35041 gatgacataa tcagcatccc ggtaggcttc atttttatct aatgtggcat taaagtgtat
    35101 tttatctgat tgcaaaaact gctgaatttc cttatcaaca ataggagata tccgatcatt
    35161 cagcatagca acgcgtgacg gtaaaatatc taatgccaca acctcatgat tttgtgcgat
    35221 tagaagcccg tttgacaagc ctacatagcc agtaccggaa atggtgattt tcatttcatg
    35281 ctctcagaat taacttaact gtgaatcatg atgtttttag catcctgata agagctaaat
    35341 gttttaacgc tacgttatta gctggtcaac gctacgaaaa acaattcaga ttttctttat
    35401 aag
//
LOCUS       DAZRUF010000014        34876 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZRUF010000014 DAZRUF010000000
VERSION     DAZRUF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.014"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHXOL_000582"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHXOL_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR6807648.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHXOL_000583"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHXOL_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR6807649.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHXOL_000584"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHXOL_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR6807650.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHXOL_000585"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHXOL_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR6807651.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHXOL_000586"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHXOL_000586"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR6807652.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHXOL_000587"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHXOL_000587"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR6807653.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHXOL_000588"
     CDS             complement(6574..7338)
                     /locus_tag="ACHXOL_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR6807654.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHXOL_000589"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHXOL_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR6807655.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="ACHXOL_000590"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="ACHXOL_000590"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR6807656.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="ACHXOL_000591"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="ACHXOL_000591"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR6807657.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="ACHXOL_000592"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="ACHXOL_000592"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR6807658.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="ACHXOL_000593"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="ACHXOL_000593"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR6807659.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="ACHXOL_000594"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="ACHXOL_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR6807660.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="ACHXOL_000595"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="ACHXOL_000595"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR6807661.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHXOL_000596"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHXOL_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR6807662.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="ACHXOL_000597"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="ACHXOL_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR6807663.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="ACHXOL_000598"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="ACHXOL_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR6807664.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHXOL_000599"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHXOL_000599"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR6807665.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHXOL_000600"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHXOL_000600"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR6807666.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="ACHXOL_000601"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="ACHXOL_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR6807667.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="ACHXOL_000602"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="ACHXOL_000602"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR6807668.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHXOL_000603"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHXOL_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR6807669.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHXOL_000604"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHXOL_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR6807670.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHXOL_000605"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHXOL_000605"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR6807671.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="ACHXOL_000606"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="ACHXOL_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHXOL_000607"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHXOL_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR6807672.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="ACHXOL_000608"
     CDS             complement(31717..33003)
                     /locus_tag="ACHXOL_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR6807673.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="ACHXOL_000609"
     CDS             complement(33054..33995)
                     /locus_tag="ACHXOL_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR6807674.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="ACHXOL_000610"
     CDS             complement(34010..34780)
                     /locus_tag="ACHXOL_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR6807675.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         8304 a         9704 c         9157 g         7711 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggcg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caggagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtctg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttc acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacgtc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcacc
//
LOCUS       DAZRUF010000015        32622 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZRUF010000015 DAZRUF010000000
VERSION     DAZRUF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.015"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            120..262
                     /locus_tag="ACHXOL_000611"
                     /pseudo
     CDS             120..262
                     /locus_tag="ACHXOL_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            424..744
                     /gene="cyaY"
                     /locus_tag="ACHXOL_000612"
     CDS             424..744
                     /gene="cyaY"
                     /locus_tag="ACHXOL_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR6807676.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="ACHXOL_000613"
     CDS             complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="ACHXOL_000613"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR6807677.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            3717..4658
                     /gene="hemC"
                     /locus_tag="ACHXOL_000614"
     CDS             3717..4658
                     /gene="hemC"
                     /locus_tag="ACHXOL_000614"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR6807678.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4655..5395
                     /gene="hemD"
                     /locus_tag="ACHXOL_000615"
     CDS             4655..5395
                     /gene="hemD"
                     /locus_tag="ACHXOL_000615"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR6807679.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            5417..6646
                     /gene="hemX"
                     /locus_tag="ACHXOL_000616"
     CDS             5417..6646
                     /gene="hemX"
                     /locus_tag="ACHXOL_000616"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR6807680.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            6649..7845
                     /gene="hemY"
                     /locus_tag="ACHXOL_000617"
     CDS             6649..7845
                     /gene="hemY"
                     /locus_tag="ACHXOL_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR6807681.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            8507..8644
                     /locus_tag="ACHXOL_000618"
                     /pseudo
     CDS             8507..8644
                     /locus_tag="ACHXOL_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(8752..9987)
                     /locus_tag="ACHXOL_000619"
     CDS             complement(8752..9987)
                     /locus_tag="ACHXOL_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR6807682.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(10134..10210)
                     /locus_tag="ACHXOL_000620"
     tRNA            complement(10134..10210)
                     /locus_tag="ACHXOL_000620"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10174..10176),aa:Pro,seq:tgg)
     gene            complement(10253..10339)
                     /locus_tag="ACHXOL_000621"
     tRNA            complement(10253..10339)
                     /locus_tag="ACHXOL_000621"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10303..10305),aa:Leu,seq:cag)
     gene            complement(10360..10435)
                     /locus_tag="ACHXOL_000622"
     tRNA            complement(10360..10435)
                     /locus_tag="ACHXOL_000622"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10400..10402),aa:His,seq:gtg)
     gene            complement(10494..10570)
                     /locus_tag="ACHXOL_000623"
     tRNA            complement(10494..10570)
                     /locus_tag="ACHXOL_000623"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10534..10536),aa:Arg,seq:ccg)
     gene            complement(10673..12058)
                     /locus_tag="ACHXOL_000624"
     CDS             complement(10673..12058)
                     /locus_tag="ACHXOL_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR6807683.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(12249..12989)
                     /gene="wecG"
                     /locus_tag="ACHXOL_000625"
     CDS             complement(12249..12989)
                     /gene="wecG"
                     /locus_tag="ACHXOL_000625"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR6807684.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(12992..14344)
                     /gene="wzyE"
                     /locus_tag="ACHXOL_000626"
     CDS             complement(12992..14344)
                     /gene="wzyE"
                     /locus_tag="ACHXOL_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR6807685.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWMALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLIIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(14341..15420)
                     /gene="rffT"
                     /locus_tag="ACHXOL_000627"
                     /gene_synonym="wecF"
     CDS             complement(14341..15420)
                     /gene="rffT"
                     /locus_tag="ACHXOL_000627"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR6807686.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(15417..16667)
                     /gene="wzxE"
                     /locus_tag="ACHXOL_000628"
     CDS             complement(15417..16667)
                     /gene="wzxE"
                     /locus_tag="ACHXOL_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR6807687.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(16669..17799)
                     /gene="rffA"
                     /locus_tag="ACHXOL_000629"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(16669..17799)
                     /gene="rffA"
                     /locus_tag="ACHXOL_000629"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR6807688.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(17804..18478)
                     /gene="rffC"
                     /locus_tag="ACHXOL_000630"
                     /gene_synonym="wecD"
     CDS             complement(17804..18478)
                     /gene="rffC"
                     /locus_tag="ACHXOL_000630"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR6807689.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(18456..19337)
                     /gene="rfbA"
                     /locus_tag="ACHXOL_000631"
     CDS             complement(18456..19337)
                     /gene="rfbA"
                     /locus_tag="ACHXOL_000631"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR6807690.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(19356..20423)
                     /gene="rffG"
                     /locus_tag="ACHXOL_000632"
     CDS             complement(19356..20423)
                     /gene="rffG"
                     /locus_tag="ACHXOL_000632"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR6807691.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(20423..21685)
                     /gene="wecC"
                     /locus_tag="ACHXOL_000633"
     CDS             complement(20423..21685)
                     /gene="wecC"
                     /locus_tag="ACHXOL_000633"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR6807692.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(21682..22812)
                     /gene="wecB"
                     /locus_tag="ACHXOL_000634"
     CDS             complement(21682..22812)
                     /gene="wecB"
                     /locus_tag="ACHXOL_000634"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR6807693.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(22868..23914)
                     /gene="wzzE"
                     /locus_tag="ACHXOL_000635"
     CDS             complement(22868..23914)
                     /gene="wzzE"
                     /locus_tag="ACHXOL_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR6807694.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(23926..25029)
                     /gene="wecA"
                     /locus_tag="ACHXOL_000636"
     CDS             complement(23926..25029)
                     /gene="wecA"
                     /locus_tag="ACHXOL_000636"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR6807695.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(25269..26528)
                     /gene="rho"
                     /locus_tag="ACHXOL_000637"
     CDS             complement(25269..26528)
                     /gene="rho"
                     /locus_tag="ACHXOL_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR6807696.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(26613..26714)
                     /gene="rhoL"
                     /locus_tag="ACHXOL_000638"
     CDS             complement(26613..26714)
                     /gene="rhoL"
                     /locus_tag="ACHXOL_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR6807697.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(26855..27184)
                     /gene="trxA"
                     /locus_tag="ACHXOL_000639"
     CDS             complement(26855..27184)
                     /gene="trxA"
                     /locus_tag="ACHXOL_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR6807698.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            27315..28580
                     /gene="rhlB"
                     /locus_tag="ACHXOL_000640"
     CDS             27315..28580
                     /gene="rhlB"
                     /locus_tag="ACHXOL_000640"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR6807699.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            28714..30198
                     /gene="gppA"
                     /locus_tag="ACHXOL_000641"
     CDS             28714..30198
                     /gene="gppA"
                     /locus_tag="ACHXOL_000641"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR6807700.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(30245..32266)
                     /gene="rep"
                     /locus_tag="ACHXOL_000642"
     CDS             complement(30245..32266)
                     /gene="rep"
                     /locus_tag="ACHXOL_000642"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR6807701.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGCKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            32483..>32622
                     /locus_tag="ACHXOL_000643"
     CDS             32483..>32622
                     /locus_tag="ACHXOL_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6807702.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         8229 a         8816 c         8182 g         7395 t
ORIGIN      
        1 tcatcgcagg agaagacgta aaagcaagcg tttagcgact ctttatgaag tctgaaaagt
       61 gaggaaatgc tgcgagtata acgcttactc gtggggattt atgttttgct ggtagtttcg
      121 tgatcggcac ctcgcgccag gatggctcta tgcactatcg caatgtggtt tgtttgttgt
      181 cttttttagt tccgtcagcg tgaggatgct cgctcggtaa acctgatcat aatgtttacc
      241 agaaacaggg aaaggcgtgg tgtaactgcg ccctaatgaa gagacaaatc tttcgctaca
      301 cagagacatc attattccgg tgacggatga atcgtgccgg aacgcagccg cagaggtgca
      361 aactttgaag ccctgacccc atgccgctat actgccgcca tcacttaaaa acaggataca
      421 acaatgaacg acagtgaatt tcatcgcctg gctgatcaac tctggctgac cattgaagaa
      481 cgcctggacg actgggatgg cgacagcgat atcgactgcg aaatcaacgg cggcgtgctg
      541 accattacct ttgaaaacgg cagcaaaatc attatcaacc gccaggaacc gctgcaccag
      601 gtatggctgg caaccaaaca aggcggctac cattttgatc tgaaaggcga tgagtggatt
      661 tgcgatcgca gcggcgaaac cttctgggat ttgctggaac aggcggcgac gcagcaggcg
      721 ggtgaaacag tcagtttccg ctaagattgc atgccggata agcctcgctt tccggcacgt
      781 tcatcacgaa aaatattgct gtaatagcgg cgtatcgtga tcctgattgg caggcggcat
      841 gttaccgata gattttgtgc ggaacggaat cacctgttca cgaccatcaa ccttcacaat
      901 ctgatagaac tgcggcaggt tgaagttgat gaagcttgag ccgtaggtga agcggtcgtg
      961 cgatgacgag tagaagcgac tgacgtcacg caccagttcc tctttgctgc cttcgcagtg
     1021 gtgatacacc tcaacccggt tgctttcgtc aagaatgtag atattaaagc cattctcgtc
     1081 ttgcgtttct tcgaagaaga actggatgat cccttcacta gcaaagccgt ccaccaccgc
     1141 cggtaatttg acgtgattgg tttcaacctg cactgacagg ccgtgcagtt tgttatgcga
     1201 aatagcgcca taaaactcga tggcgttctc cagcttctgt accgatacat tcaggcgttc
     1261 gaagaacaag ccccaggttt gaccagaaac gcgcagcgcc ttgaaacgcc cggtttcctg
     1321 gcgggtgctg gaaaggcgca gttcaataca ctcagaaacc agttgctgca cgcgagtacg
     1381 gattaacccg cgcagatgct ggctataaca gaagacttcc acgctatctg gcggtgcggc
     1441 gtcctgatgc attttgccga gaatagtttt cagggcttcg atcatcgatt gctcgccgtt
     1501 gaagtgcagc gtacgcactt cgttccacga gttgcggtac agcaggtcaa cgctacctac
     1561 caggcaattt tgattctcgc caaagctgaa gacatccagc ttacggaaat caaaatgcac
     1621 cacctgattg cggaacgccg ctgtcgggtc atattccagg ttaacgataa tcgccagatg
     1681 gcggatctca cacgggctgt agagcgcctt cggtgtcggt gcaggtaagc gcagcgggaa
     1741 atggtgcgac acgtcggcga ccatctcctg caacttaggc aaatcgacaa tgccgttacc
     1801 tttaatatac aaacgggtgc gcgaggtcag cagaccgtta aaccatgccc acgccaccag
     1861 tttattcagg taacggttat attccagcgg ctgatggctg atgatcgact caatatttgg
     1921 cgcgcggtta tacagatacc aacctgaacg gttagcccgg cccggcggca cataaataaa
     1981 ggtcagattc ggttccgaga gatcgggtga aatctgcggg tttaccagcg tcactttacc
     2041 tggtaatgct tcaaacgcgg catacagctt acgcgtcagc acgccgatat cctgcggact
     2101 ggcggagacg ctaaggttat tgcgacgcgc aaagcggatc agattacggt agctctgcat
     2161 catcgcgtcg agcaactcgt tgtgcgcctc acgcacctga tcaatcttcc agttagcacg
     2221 gttatcgagc attgccagac gagcttcgtc ccaaccccac tcgctcacta actggctcaa
     2281 cactgcgcga cgccagccta cacaggcgcg ttcacggctg agtttttcgc acacttttaa
     2341 atagaagcag cgacgtacta aatccagacg ggtgaaatct tcaatcgccg tcaggtattc
     2401 agtaacacgc tccagcatca tgcagtatgg atcgaggcca aacgatacaa tctcgccgtc
     2461 gtgcaaacgc tgtttgatat ctttcgccag cagacgtggg ttcgggtatt cccaggaata
     2521 ggcttccagc agcagtgttt tcagcaccgc tttgtatggg gaatcgatac tcttgtagag
     2581 ctgccaaagg ctggcaccaa agtactcttc agcagaaagc gagcttaagc caccgagatc
     2641 cagccattca tttggcgtca gcacgccctg cgcgtaaagc gtcatcacat agtcgtcgta
     2701 atgctcttct tcgtcgcacg gcaccatatt ccacagaata cgcttaccgg cgagacgcac
     2761 ggcggtacga taaaattcgt caagcagcag tatatgctgg gtggagccac aatcttcgcc
     2821 ccccaggctg ccgctttcat tatgacgaaa gcggttttca tcaatcagga agaagctgac
     2881 ttccacaccc agcgaggcgg cccagctttc cagcaggctg catttacgtt gtagtaactg
     2941 gcgctcttcg ctatcgagcc aggattgatg acagacccag atatccaggt cagaggaaca
     3001 actttgccct acggacgagg tgctgcccat ggtgtataca ccagtaattg gaagctcacc
     3061 tttcggtgga tcctgtactg acattccacg atacagttca agctcgttca ggtagtggcg
     3121 ttgagtttca tcaggcgtgt aaaggcaaat gcctttggga acgttaccat caaggtaacc
     3181 cggcattagc ggatggtgat agtgcaacaa tgtcggcagt agactgtaga cctgttggaa
     3241 tgcaggcccc atagcagcaa gcgcgcgatc cacacgcaat tgatttatgg catccagtct
     3301 ctgtttcaga gtctcaatat agaggtacaa gacgtatcgc ctgatttgct acccgtcatg
     3361 actgtgattc cgccaacatc aacggtaaca cgcggcattc gggatatttc gtatgtcaaa
     3421 ggtaaccgtt accacttttc gcgcctggtt tttttagttt cacgacgaaa aaatggtcta
     3481 aaacgtgatc aatttaacac cttgctgatt gaccgtaaag aaagatgcgc tacatacaag
     3541 tgtagcaccg tttattctct gtaaattcct tattacgaca gcgtgaaacg tctgtcagga
     3601 tccactgcca gacctcattt tacggtttgc gcaggcgtct acgtttcacc acaacactga
     3661 catcactctg gcaaggatgt taggatggac cacggatgat aatgacggta acaagcatgt
     3721 tagacaatgt tttaagaatt gccacacgcc aaagcccact tgcactctgg caggcacact
     3781 atgtcaaaga caagttgatg gcgagccatc cgggcctggt cgttgaactg gtaccgatgg
     3841 tgacgcgcgg cgatgtgatt cttgatacgc cgctggcgaa agtaggcgga aaaggcttat
     3901 ttgtaaaaga gctggaagtc gcgctcctcg aaaatcgcgc cgatatcgcc gtacattcaa
     3961 tgaaagatgt gccggttgaa ttcccgcaag gtctgggact ggtcactatt tgtgagcgtg
     4021 aagatcctcg cgatgccttt gtgtccaata actatgacag tctggatgcg ttaccagcag
     4081 gcagtatcgt cgggacgtcc agtttacgtc gccagtgcca actggctgaa cgccgcccgg
     4141 atctgattat ccgctccctg cgcggcaacg tcggcactcg cctgagcaaa ctagataacg
     4201 gcgaatacga tgccatcatt cttgccgtag ccggactaaa acgtttaggt ctggagtccc
     4261 gcattcgcgc cgcgttacca cccgagattt ctcttccggc ggtaggacaa ggtgcagtcg
     4321 gtattgaatg ccgccttgat gatacgcgca ctcgcgagct gcttgccgcg ctgaatcacc
     4381 acgaaactgc actgcgcgtt accgcagaac gcgccatgaa tacccgtctc gaaggcggat
     4441 gtcaggtgcc aattggtagc tacgccgagc ttattgatgg cgaaatctgg ctgcgtgcgc
     4501 tggtcggcgc gccggacggt tcgcagatta ttcgcggtga acgccgcggt gcgccgcaaa
     4561 atgccaaaca aatggggatt tcgctggcag aagagctact gaataacggc gcgcgcgaga
     4621 tcctcgctga agtctataac ggagacgccc cggcatgagt atccttgtca cccgcccgtc
     4681 tcccgctgga gaagagttag tgagccgtct gcgcacactg gggcaggtgg cctggcattt
     4741 tccgctgatt gagttttctc cgggtcaaca attaccgcaa cttgctgatc aactggcagc
     4801 gctgggggag agcgatctgt tgtttgccct ctcgcaacac gcggttgctt ttgcccaatc
     4861 acagctacat cagcaagatc gtaaatggcc ccgactacct aattatttcg ccattggacg
     4921 caccaccgca ctggcactac ataccgtaag cggacagaag attctctacc cgcaggatcg
     4981 ggaaatcagc gaagtcttgc tacaattacc tgaattacaa aatattgcgg gcaaacgtgc
     5041 gctgatatta cgtggcaatg gcggtcgtga gctaattggg gataccctga cggcgcgcgg
     5101 tgctgaggtc actttttgtg aatgttatca acgatgcgca atccattacg atggtgcaga
     5161 agaagcgatg cgctggcaat cccgcgaggt gacgacggtc gttgttacca gcggtgaaat
     5221 gttgcagcaa ctctggtcgc tgatcccaca atggtatcgt gagcactggt tactacactg
     5281 tcgactattg gtcgtcagtg agcgtttggc gaaactcgcc cgggaactgg gctggcaaga
     5341 cattaaggtc gccgataacg ctgccaacga tgcgctttta cgggcattac aataactctc
     5401 ataacaggaa gccataatga cggaacaaga aaaaacctcc gccgtggttg aagagactag
     5461 ggaggccgtg gacaccacgt cacaacctgt cgcaacagaa aaaaagagta agaacaatac
     5521 cgcattgatt ctcagcgcgg cggctatcgc tattgctctg gcggcgggcg tcggtttgta
     5581 tggctggggt aaacaacagg ccgtcaatca gaccgccacc agcgatgccc tggctaacca
     5641 actgacggca ttgcaaaaag cccaggagag ccaaaaagcc gaactggaag gcattattaa
     5701 gcaacaggct acgcagcttg aacaggcgaa tcgtcagcaa gaaacgctgg caaaacagct
     5761 ggatgaagtc cagcaaaagg tcgccaccat ttccggcagc gatgctaaaa cctggctgct
     5821 ggcccaggcc gattttctgg tgaaactcgc cggacggaag ctgtggagcg atcaggacgt
     5881 cacgaccgct gcagcgttgc tgaaaagtgc agacgccagc ctggcggata tgaatgaccc
     5941 gagtctgatt accgttcgtc gggcaattac cgatgatatc gccagccttt ctgcagtatc
     6001 gcaggtggat tatgacggca tcatccttaa gcttaatcag ctttcaaatc aggtagataa
     6061 cctgcgtctg gccgataatg acagcgatgg ttcgccgatg gattcagacg gtgaagagct
     6121 ttccagttcc atcagcgaat ggcgtatcaa tctgcaaaaa agctggcaga actttatgga
     6181 caacttcatt acgattcgcc gtcgtgatga caccgccgta ccgctgttag cgccaaatca
     6241 ggatatctat ctgcgcgaaa atattcgctc tcgcctgctg gtcgcagcac aagctgtacc
     6301 gcgtcatcag gaagagactt atcgccaggc gctggagaac gtctccacct gggtacgtgc
     6361 ttactacgat actgatgatg ccaccaccaa agcgttcctc gacgaggtgg accagttaag
     6421 ccagcaaaat atctcgatgg atcttccgga aaccctgcaa agccaggcga tgctggaaaa
     6481 actgatgcag acccgcgtgc gtaacctgct ggcacaaccg gcagcgggga caacggaagc
     6541 taaacctgca cctgcacctg cacctgcacc tgcacctgca cctgcacctg cacctgcacc
     6601 tgcaccgcaa ggtgatactc cggcagccgc gccgcaagga gaataaccat gctaaaagtg
     6661 ttattactct ttgttttgct gattgcgggg atagtggttg gcccgatgat tgccggtcat
     6721 cagggttacg tgctgatcca gactgacaac tacaatatcg aaaccagcgt cacgggcctg
     6781 gcgattattt tgattctggc gatggtagtg ctgtttgcca ttgagtggct actgcggcgg
     6841 atcttccgca caggcgcaca cacccgtggg tggtttgtcg gacgtaagcg tcgccgtgcc
     6901 cgtaagcaga ccgaacaggc gctgctgaaa ctggcggaag gcgattatca gcaagttgaa
     6961 aagctgatgg cgaaaaatgc cgatcacgcg gaacaaccgg tggtgaacta tctactggct
     7021 gccgaagccg cgcaacaacg tggtgatgaa gcacgcgcca accaacatct ggaacgcgca
     7081 gcagagctgg ccggcaatga caccattccg gtagaaatca cccgtgtacg tctgcaactg
     7141 gcccgtaatg aaaaccatgc tgcacgccac ggcgtggata agctgctgga agttacgcca
     7201 cgccatccgg aagtattacg tctggcggaa caggcgtata tccgcacagg tgcatggagt
     7261 tctctgctgg atatcatccc atcaatggcg aaagcccatg ttggtgatga agaacatcgt
     7321 gcaatgctgg aacaacaggc atggattggc ctgatggatc aggcgcgtgc cgataacggt
     7381 agtgaaggtt tgcgtaactg gtggaaaaac caaagccgga aaacgcgtca tcaggtagcg
     7441 ttgcaggtgg caatggcgga acatcttatt gaatgtgacg atcatgatac tgcccagcaa
     7501 attatcatcg atggcctgaa acgccagtac gacgatcgcc tactgctgcc gattcctcga
     7561 ctgaaaacca acaatccgga acagctggaa aaagtgctgc gccagcagat caaaaacgtc
     7621 ggcgatcgcc cgctgttgtg gagcacactg ggccagtcac tgatgaagca cggagaatgg
     7681 caggaagcat cgctcgcctt ccgcgcagcg ctgaaacaac gtccggacgc ctacgattac
     7741 gcatggcttg ccgacgcgct ggacagactg cacaagccgg aagaagctgc agcgatgcgt
     7801 cgcgacggtt tgatgttaac gttgcagaac aacccaccac agtagttcct tctcacccgg
     7861 aggcaagcac ctccgggacc tttctgatgc ataaaaaaac gcctgctctt attacggagc
     7921 aggcgttaaa acaggtctgt atgacaacag tgggtgcttc actcaacgtt gtgtccatgg
     7981 tgtctgatga ggcataagcg acatctgtca gtggacgata agcaccgtaa acggctctgc
     8041 gtcattccgg agtttatgag gcactaaggc gaacataaga gatggaatga gcatctaccc
     8101 gtttattatg ccacagagaa tcgggaaata acatccctta acacttgtta tgagataatt
     8161 ctgtaatcct ctttgcttcc tgagtgaata tttaacctga gcttgatcct acacatactt
     8221 attatgaatg ataaaattca ttcaattaat aacacatata ttaattgccg ttaaaactaa
     8281 aaaagcatta ataatcaacg caatataata aacctgcctt acatatcaac tgcgccagag
     8341 gtaggattga aaacgctctc ctgattttcc aattcatttt ctggataaat aaataattta
     8401 tttttgtcac tattatttat gtaatcatcc tgtcagggag agggatctca attatcaatg
     8461 cttaattacg tcatcatttt gatgacatga aaccggagca ttttgcatgg aattttcgtt
     8521 ttcacccaaa cttcttgttg ttgctgtcgc cgccgctctt cctctcatgg ccagcgcagc
     8581 agataccccg tcaactgcca ccgcacgcaa aggctttgcc ggatacgatc acccaaacca
     8641 gtatccacag aacgctgcaa tttattgaat ttgcacgatc atgtaggccg gataaggcgt
     8701 ttacgccgca tccggcaatc aaccgcaggc ggccacaaaa tggccgcttt tttacttatt
     8761 caccaccagc aaatgtgcct gcataatgtc actggccggg cgaccgtgcg ccagcaaatc
     8821 agccattgct ttaagatatg gcggtagatg gcggaaataa cgctgatacc cggcacacaa
     8881 ataattcagc cccggtttgc cgctggcatc gagcatgaag cggtgtttcg ggcagcctcc
     8941 ccagcacgct tttaacacgt tacaactgcg acactgcgcc ggtaattgct taaatttatc
     9001 ttcaccaaac acctgctgtt gcggggaatc gatcatttct gcaattgttt gctgatgcat
     9061 attccccagc cgatattgcg gataaacata gtgatcgcag gcgtaaacgt cgccgttgtg
     9121 ctcaacaatc accgagcgcc cacaggttgg ctgatgatgg caaaccgcac ccggcgcacc
     9181 gacaaaattg gcaaacgccc attcgatatt catcacgaaa atcttgccga cgtcgcgttt
     9241 gatccagtgg tcgaatatcg ccaccagaaa ttcaccaaat tcatcggggc gcaccgacca
     9301 ttccgtcagt tccccctgaa tatcaccagg cgcatgcaat ttcagtccat cgcgggcagc
     9361 tgtttcatca gccagacgct cgaccaccgg aataaactgg atgaattcga ctcccgcatc
     9421 gcacaaaaaa tcataaacct gcaacggttg ctgcgcgctg gtgcggttga cgcagaccag
     9481 cacgttatag tcgacatgat gtttttgcag cagcgtcagg gcacgcatca ccagcttatg
     9541 cgttggtctg ccacctttgg tcacgcgata ttgattgtgg atctcagccg gaccgtccag
     9601 cgataaccca acaagaaaat gattttctgc cagaaatgca caccatttat catcgagcag
     9661 cacgccgtta gtctggaagc tgttacttat cttcctgcca gcaccgtatt tcgcctgtaa
     9721 ctttacggca cggcggtaaa aatccagccc gagtaacgtt ggttcgccgc cctgccaggt
     9781 aaaagccact tcgttttgcg gttcgctggc agcgatatag tgacggacat acgcttccag
     9841 cgtgtcatcg tccatatgcg tgactggctt ttcgcggtaa agggattgtt tttcgagata
     9901 aaaacagtag tcacagttca gattacaatc ggaaccactc ggtttcgcca tcacatgaaa
     9961 agcacgcgtt ggaacctgtt gcagcatggt cactccttgc ctgaagtcat ccttcaatac
    10021 gttaagggcg aaatcatacc gtggggcaat gagttattac gggcgtttat tgcgaaggga
    10081 gggtgacgaa atgcacagaa aacaaaaaac cccgccgaag cggggttcaa aattggtcgg
    10141 cgagagagga ttcgaacctc cgacccactg gtcccaaacc agttgcgcta ccaagctgcg
    10201 ctactcgccg aaatactgct ttttgaattt ttagttcaat tctttaaagt cgtggtgcga
    10261 ggggggggac ttgaaccccc acgtccgtaa ggacactaac acctgaagct agcgcgtcta
    10321 ccaattccgc caccttcgca ttgtcacaac ttctaataat ggggtggcta atgggattcg
    10381 aacccacgac aactggaatc acaatccagg gctctaccaa ctgagctata gccaccacta
    10441 caaatcttgt tacgcggtat tactaccacc gcagctcaag cgccgggact aaatggcgcg
    10501 cccgacagga ttcgaacctg agacctctgc ctccggaggg cagcgctcta tccagctgag
    10561 ctacgggcgc ttagcgccgt tgcgggggtg gataatacgg acttcccacc cctctgtcta
    10621 gtcccttttt aaataaattg cgcgtttggt tacggtttgt gcgttttgct gcttattcct
    10681 ccagtttatg cgctttcccg tggcgattaa ggccaaaaac tttataaatc gccgtcaccg
    10741 ccagcataaa gatgatgcca acaaacagcg acatacgcgt atcttcatta aagtacatgc
    10801 cgatcaaaac gcaaatcagg aatgccattg ttacgtagtt ggcccacggg aacaggattg
    10861 agcggaacgg atggctggca atcgccgctt tatgtgcacg ccgaaaacgc agctggctta
    10921 tcaatatcac aaaccatggc accatccccg gaagcacgct ggcactgtag acgtagacaa
    10981 acacacgctg cggattggga atgatgtagt tcaggcatga gccaattagc agaatagcaa
    11041 tagatactgc cacacccgca actggtacgc cgtgacggga aactttcgcc atcgccgccg
    11101 gtaactgacg gtttttcgcc agtgcgtaga gcatacgtcc gcaactgtac atgccgctgt
    11161 tacagccaga gagcgcagcc gtcagcacca caaagttgat aatgcccgcc gctgcggtaa
    11221 taccgatttt ggcaaaagtc agtacgaacg ggctgccgtt gctgccaatt tcattccacg
    11281 ggaagatggt gacgataacg aaaatcgcgc ctacgtagaa aatcaggatc cgccacagca
    11341 ctttgcctac agcactgcgc agcgtcacct gcggattctt cgcttcaccg gcagtaatgc
    11401 caatcagctc cacgccctgg taggacgcca ccacaataca cagcgcggtc aggaaccctt
    11461 tccagccacc cgcaaagaaa ccgccatgct ctgtgagatt gctaaaacca atcgactgcc
    11521 cgccattgcc aaagccaaag aaaatcacgc ccaggccaat gacaatcatc acgataatcg
    11581 tggtgacttt gatcatcgcg aaccagaact cgatttcgcc gtacaaccgc accgccgcca
    11641 gattcgccaa cgccaccagc gccaccgcga tcaatgcagg tatccactgc gccatctccg
    11701 ggaaccagaa ctggacataa acgccaatgg cggtgatttc tgagatcccc accgccatcc
    11761 acataaacca gtaagaccag gcggtgagat aaccaaaaaa cgggctcata taacgatgcg
    11821 cataaacggc gaacgaaccg gtaacaggtt caaggaacaa catttcgccc attgaacgca
    11881 tgatgaaaaa gacgaacagc ccggcgatga tataggccaa caatacggat ggcccggccc
    11941 atttcagggt actggcggcc cccataaaca ggccgacgcc aatagtgccc cccagggcga
    12001 tgagttcgat atgtcgagct tccagcccac gctgtagctc tggtttgtta tctgccataa
    12061 atcctcgtgt tgtgtttgca tgctttccgg tgttaccggt tatcgttatg ggtacatcga
    12121 gtgttgcgaa tgttttcgta attcaggaga aatggcaaat aaagcattaa aaatttgaat
    12181 gctttgtgta ataaaaaagc agacaggcga cggagtgacc actccgtcgc tttacaaaga
    12241 gaggaaaatc agaggttgcc ggtgtagtgc cagcgtaaat aacgcagcaa acgaagctga
    12301 cgcttaatgc ggctcggctg cgaaagcagg cggtagagcc actccagtcc cagcgtttgc
    12361 cagattttcg gtgcgcgttt tacgtgaccg gtgaaaacat cgtaagtccc gccaacgccc
    12421 atatacagcg catctggatg caccagacgg cagtcgcgca tgaagatctc ctgctttggc
    12481 gatcccatcg caacggtgac gatttgcgct ccgctggcat gaatgcgttc aaacagcgcc
    12541 tgacgctgct cgggtttaaa ataaccatcc tgactgccaa cgatattcac attccattgg
    12601 ttgcgcagtt tagcttcagt ttgcgccagc acttcaggtt taccgcccac aagaaatacc
    12661 ggcgtccctt ctttgcctgc gcgcgccatc agctcttccc agagatcggc accggcaacg
    12721 cgggaaacct gcgcctgcgg gtacttttta cgtactgaac gtacaacgct gatgccatcc
    12781 gcatatttaa attcggcagc gttaattaac tccctgacct cggcgttatc ttcaatggtc
    12841 agcatttttt cagcattaat ggcaaccagc gttccctgct taagctgccc gtcagcaaac
    12901 agataatcga gggcgtgctg catatcacgc caaccaatca actgtaagcc acgcagcgta
    12961 taggtcggtg ccgtggtgtt gttattcatt gttatccttc aacctgcgtc cggagcgatg
    13021 attttgtacg tttatgaatg agtccggcgc tttcaaaaag ccagtacagc agttttgcga
    13081 ttatcagaca tgcgccgaag accacgataa aaaagactac gcgtgagaca aacgaatcca
    13141 gcccttcacg cgccagcacg atcatattga agatcgcccc aaagcagaaa ctgtgcaata
    13201 tcgcagcctt atagcgatta ggctcgcggt tgcccagctc atacagccag tcgaaccatt
    13261 tgatgatcag accaaccacg atcgccccga gcgggatgaa caacgcgccg cccatcacca
    13321 ccagtgagcc tataagcgta ggcgagatcg ccagtccgga gtggttattc agcacttccc
    13381 aggtaaagta gttggctgag ttcagcacca tactcgggcg acccggccac agccaggaag
    13441 ggataaagac atagaaatcg cggacaattg gagccaggcc ctggaagtcg atgttgtcgt
    13501 agttctgcaa cagcaacgcc agattctccc acggggagaa ggtgtcgcga gtgagataga
    13561 gaaacgtata gaacgcttca tcgccgctca cattcattcc atagcgtttt agcgccatcc
    13621 agaacatgcc aacaatcccc agcacgcccg ccgccgccag catccacaac gaaatccagc
    13681 cgcgaataat gccaataaac aggaagatag cgaatgcgat gatgatattg gcgcgagtgc
    13741 cgccgacaat catataagtc agcaagccaa aggcgaccgt gctgacgagg aaaaacagcc
    13801 acgctttgct gtcctggcgc agaaagtaga ccaccagcat cgccgggatg aaaaagtaaa
    13861 agaagcgttt taacgccacg ccggagactt cactggagaa gatctgactg taggagttaa
    13921 gccggaacag caaaaagccg ttgtgcatga agaagatgcc gacgcttacc agcgcgatac
    13981 ccatcaggat cacccacgta agattggtct ccacgcggtt catggtaaac agcggacggc
    14041 gcggtacatc agcaacgcgt ttgcgtaggc gggttttgta ggtgacatag taaaccgcgt
    14101 agaagcagcc cgcagaaagc aacgcctgca acaagatttc tggcggcgcg acaccaacat
    14161 caaagcgaaa taccagcacg ctggtcagcg ggaagccgaa gaaaaaggtg agcaaaaaca
    14221 gcaatgaaaa gaagacattg aagttaaagc gcacacggcg aaactcaaac caggtcagcg
    14281 tggcaataaa cagcgtgcag agcagccaga ccacaaacag gccactgaat tgcagcagac
    14341 tcatgcgacc tccccggcgg caatcgccaa cgcccgctgc cagccttgta gatagttagg
    14401 gctaaagaag gcaatggtgt ttttatccac cgacgccaac tggcgctgcg cttcacgcac
    14461 aatatcctcg ttgagatcgt cggtagtaaa cagcaccggc aaatgctgtt ccgtcatatc
    14521 ctgccagaac ggattttccc ggttaagcac acaaggaatg cccgcctgaa tcagtaagca
    14581 aagcgtacca atgccctgct ggcgggcaaa aataaagtaa ccaagatcgc actgacgaag
    14641 tagcgccaga taggcgtcaa attccagttt ttcgctcaaa atttgcagat tttcttcgct
    14701 gaataactcc agccccgcct gacgaacttc ctcaatgtac gcttcgttat taggcggata
    14761 tcccatcggc accaccactt ttaccgtatc gccaaattgc tgatgaacgg cgcgcaaggc
    14821 agcaatatgg tcattgctgc ggtcaccgga gttccccacc agaatggtca ttttcccttc
    14881 acgttgccga tcattcgcca tcgtattgag agaagggtcc atccgcgtcg ggaagtacag
    14941 cagttcgccc cgcacctttg gatgcgtttt ggcaaaaaag ctcaaatcac cgcgggtggc
    15001 aaatacacag ccgactcgct tttgcgccag gcgacgtagc gggtaaaaaa gcttatatct
    15061 caagccactg gaaagctcat acaggtctgc cccccagata tgccagaaaa actggctggg
    15121 cttaatgcca ccactcagca gagccagcca cagtgtggga ttgaactgac cgtggaaaaa
    15181 aaatcgctgc tgacggttag cttttgcttt cgcgatgacc gcttccgcca gcgatttttt
    15241 cccagggaaa aattgcacag aaagcgccgg acagctatca cttaagccgt cgtccttgcc
    15301 aacaaccata aactcgcgcg cgtgctcgct cgtcgcggcc agcgcgtcat tgaaaaaccg
    15361 caaaacggtt cggttatggt gagggatatc cgatcccagt acgtgaatca gtacagtcat
    15421 gcccgcctac gccagagtaa aaacacgcca caacaaagag aaaaatagac gatataagtt
    15481 gccatatatg cctgcgccgc gcccagtgca ccatgcgcag ggattagcca gtgggcaaat
    15541 accatcaata aagtgaactg gctgacttcc gccagaatat aaaaccgcag tgacgctttg
    15601 gcgatcacca gataaccaaa gacataagcg cccactttta acacatcacc cactaactgc
    15661 caggcaaaga gatcgcgcat agcggtaaat ttattcgaca acagcaacca gatagcaaaa
    15721 tcacgcagca accagacggt aaaactcgcc gccgccactg ccggtaagac gaatttcagc
    15781 gatttaacca cttcccgggt gatatcgcgc ttttccgtta gccgcgacaa cgtgggcagc
    15841 aaatatacgc tgaacgatgc cgtaataaat tgcaggtagg catcggaaat actgctcacc
    15901 ccttgccaga tccccacttc atcccagcta tactgcgccg ccagcagttt acgcatcatg
    15961 atgtaagcaa caggcaaggt caccgacgta atcaacgcca tgagcgtaaa tttgctcaac
    16021 tgccctgcca gaccgttatc ccagctgggt ttcagatagc ttaacgggat gacaccacgt
    16081 ttgatcaaca tgatggcggc aggaatcacc accagcgcgg gaatcagcgc cagacccagc
    16141 aacgcccctt cataaccgcc caaacggtaa ctgacgtagt acgcgagaac gccaatcaag
    16201 ctgccgacaa tcagcgataa cgcattacct gcggcatcgc gaaagccttt catcagcgcc
    16261 agtaacaggt tgccccaggc gatccccatt tgcaccagcg ccactaaacg caccagcccc
    16321 tgatagtcgg tattaccaaa cagtccctgg ctgattggcg cagctgccag cacaaaaacc
    16381 agcgccatca gcgtagagaa accaagtacc atcgctgatg aagtgccgac cacgcggcgc
    16441 agctgttgcg gattatcatg gtactgggca acgtatttgg ttacaccgtt aaagatgcca
    16501 gctccggcaa gcacgccgag cacggtaatc aactggcgga aatttgccgc cagcccaagc
    16561 cccgccggac caaatgacac agccagcaac ttaccgacca gtaacccggc accaatcttg
    16621 accagtgtac tggccgccgt ccacaaggac gcttttgcca acgacatatc aggagaagta
    16681 gttcaacaaa gtcgcaatta ccgtacgctg attgacgggc gacaggttgt agaacagcgg
    16741 caggcgcagc aggcgctcgc tctctttggt ggtgtagcga tcttcaccgt ggaactcacc
    16801 aaagcgttcc cccgcagggc aaccgtgcag cggaatgtaa tggaacaccg ccatgatttc
    16861 cgcttctttc agaaagttaa tcaacgcgct ccggtcatca atatcccgca gtttaatgta
    16921 gaacatatgc gcgttctgca cgcagccatc gggaatcgac ggcagctcga tacgcccggc
    16981 tttcgccaga ggcgctaacg catcgtagta gttttgccac agcgccagac gttgctggtt
    17041 gatacgatcc gctgcttcca gttgcgccca caggtacgca gcttgcagat cggacatcaa
    17101 atagctggag ccaatatcgc gccaggtata tttatcgacc tgaccacgga agaactggct
    17161 gcggttagtg cccttttcac ggatgatctc ggctcgttcg attaacgctt tatcgttaat
    17221 cagcgtcgcg ccgccttcac cacccgccgt atagtttttg gtttcatgga agctaaagca
    17281 gccaatatga ccaatggttc ccagtgcacg ccctttgtaa gtggacatca cgccctgagc
    17341 ggcatcttct accacaaaca gattatgctt tttcgccaac gccataatgg tgtccatttc
    17401 gcaggccaca cccgcgtaat ggaccggcac gataacgcgc gttttgtcgg tgatcgccgc
    17461 ttcaatcagc gtttcgtcga tgttcatggt gtccgggcga acatccacaa aaacgatttt
    17521 tgcgccacgc agcacaaagg cattggcggt ggagacaaag gtgtagctcg gcatgatcac
    17581 ttcatcgcca ggctggatat cgagcagcag cgccgccatc tccagcgaag cggtgcagga
    17641 cggcgtcagt aacactttgg cgctgccaaa acgttgctcc agccactgct ggcagcgacg
    17701 ggtaaaaccg ccatcgccac acagtttgcc gctacccatt gccgactgca tatagtcgag
    17761 ttcggttccc accaccggcg gtgcgttaaa tggaatcatg tgatcacctg tataaccagt
    17821 acgcggtgct ttctacattc gcaccacttt gtatgtatcg tttaagcgcg gcggtgttgc
    17881 ccatttgggt cgccacccgc aaagttgttt taccgcgagc atacgcccag tttagcgccg
    17941 tttgcatcag ctcagcacct gcaccgcgtc cagccagcag gccaattcgc gcatctgtcg
    18001 cattgagttc ccgtaaagag acataaccgc gaataccgcc ggacgccgca cgtaaaatca
    18061 gacattgatg atcaaaggtg ccgcgcacgg cattttcaat ccactgtgca taaaagcgtc
    18121 cgctggcgtc aggcgcatac cacggcgcac gaaaacggct ttgcgcaaat gcggcgctgg
    18181 ctaactgacg taatgcggga atatcggtct cttgtgccac tacagcacca ctatcactga
    18241 cattgttcac gggtagtgcc aaatcaactt caccttctac cagggagaat cccagctgtt
    18301 gcagggcatc cagttcaccc gtatttgatg ccgcaatttt ggcctgcacc cgtgaccacg
    18361 gcgctaacac gtctggcgtc aggagcggtg cttcagacgt aatgcgcacg atggcgctgt
    18421 taacaccaaa gaaggcgttt tcccaggtta gtggctcaat actggcgcgg acgggcacga
    18481 agtaactcca gcagatattg gccgtagcca gttttcgcta atgaactggc agcacgcttc
    18541 acaccctcgt catcgagcca gccgttacgc caggcgatct cttccaggca ggcaatctta
    18601 aagccctggc gtttttctac tgtctgcaca aaggtactgg cttcaatcag gctgtcgtga
    18661 gtgccagtgt ccagccaggc aaatccgcgc ccgagcagtt caacggtcag gttgcccgcc
    18721 tcaaggtaca tctggttgat ggaggtaatc tccagttcac cacgctccga cggcttcacc
    18781 tgctttgcgt actccacgac tttactgtcg tagaaataaa gcccggtcac cgcccagttt
    18841 gacttcggct gttttggctt ttcttccagc gagatagcgc ggaaattgtc gtcaaactcc
    18901 accacgccaa agcgttccgg gtccatcacc tgatagccaa ataccgtcgc cccttccgtg
    18961 cgcgccgcaa catgacgcag cttcggactg aagccctgac cgaagaagat gttatcgccc
    19021 agcaccagac aagaaggttc accgttgagg aaggtttcac cgatgataaa ggcctgcgcc
    19081 agaccgtccg ggctgggctg ttcggcgtat tccagctgga taccgaactc actaccatcg
    19141 cccagcaggc gctggaaata acctttatct tccggcgtag tgatgatgag aatttcgcgg
    19201 atatcggcca acatcagcac cgacagcgga tagtaaatca ttggcttatc gtaaatcggc
    19261 aacagttgct tcgatacgcc gcgcgtaatc ggatgcaatc gggtgccgga accgcccgcc
    19321 aggataatac ctttcatgtt tgcctccgct gaaaattagc ctttcagacc taaacgctcg
    19381 ccctgatagc tgccgtcctg cacctgcttc caccagcttt cattagccag ataccactgc
    19441 accgttttac gcattccact ttcaaaggtt tcctgtggca gccagccaag ttcacgggca
    19501 attttcgaag catcaatggc ataacgcaga tcatgccccg gacggtcagc gacaaaggtg
    19561 atcaagtcac gatagtgcac cacgccgtgc ggcttgttcg gagccagttc ttccagcagc
    19621 tcgcaaatgg tttccacaac atcgagattc ttacgctcgt tgtgaccacc aatattatag
    19681 gtttcaccga ctttcccggt ggtcgccacg caatacagcg cgcgggcgtg atcttccaca
    19741 tacagccagt cacggatttg ctgcccgttg ccatataccg gcagcggttt acccgccagc
    19801 gcgttgagga tcatcagcgg gatcagtttt tccggaaagt ggtaaggacc gtagttattc
    19861 gagcagttgg tgataagcgt cggcagaccg taggtccgca gccaggcgcg caccagatgg
    19921 tcgctgctgg cttttgacgc ggaataaggg ctgctcggcg catacggcgt ggtttcggtg
    19981 aagaaatcat ccgtcgagtg caggtcacca tatacttcgt cggtggagat atgatgaaaa
    20041 cggaacgctg attttttatc ttccgtcagc gtattccagt aagcccgcgc cgcttcaagc
    20101 aatgtataag tcccgacaat gttggtttca ataaacgctg ccgggccgtc aatagaacgg
    20161 tcaacatggc tttcggctgc cagatgcatg acacagtctg gctgatgctc agtgaatacg
    20221 cgtgccagtt ctgcccgatc gcagatatca actttctcaa aggcaaagcg ctcgctttgc
    20281 gcgaccggtg ccagcgacat caggtttccg gcgtaggtca gcttatcgac cactaccacc
    20341 gcgtcgctcg tttcgttgat gatataacgc accagcgccg agccaataaa cccggcacca
    20401 cctgttatca gaatttttct catcagcgcc agactccttt ggcatcgacg acatactgct
    20461 gatggacatt gtcgccattg ataactttaa actgactatg atcgaccagc atcaccagca
    20521 cgtctgccgt tgccagcgcc tcgtcaagct gcgccagagt acaaagcccg gtcagttttt
    20581 tcggcaactg gtggatgtta ggctcaacaa ccagagtttc gccgctatgc cactgggcga
    20641 tcagttcagc gatttccatc gccgggcttt cgcgcaggtc atcaatattc ggtttaaacg
    20701 ccagaccaaa gcaggcgatt ttcagttcac tggcgcgttt atcggtagcc gccaggcaat
    20761 cagccaccgc cgctttcacc tgatcgataa cccagaacgg tttgtgatcg ttcacttcgc
    20821 gcgcggtacg gataagccgc gcctgctggg ggttctgtgc cacgataaac cacggatcaa
    20881 cagcaatgca gtgaccgccc acgccagggc caggctgaag aatattaacg cgagggtgac
    20941 gattcgccag gcgaatcagt tcccagacgt taatcccctg atcggcacaa atcagcgaca
    21001 attcattagc aaaagcgata ttcacatcgc ggaagctgtt ttcggtgagc ttacacattt
    21061 ccgccgtccg cgagttagtg acgacacact caccttcgag gaaaattttg tacagttcgc
    21121 tggcgcgggt cgaacaaacc ggcgtcatac caccaatcac gcgatcgttt ttaatcagct
    21181 cgaccataac ctggcccggt aacacgcgtt ccgggcagta agcaatgttg acgtccgcct
    21241 gctcgcccac ctgctgtggg aaagtgagat ccggacgcat ctctgctaac cattctgcca
    21301 tcttctcggt tgaccccacc ggcgaggtgg attcaaggat caccagcgcg ccttttttca
    21361 gcactggcgc aatggagcga gcagccgatt caacgtaggt catatctggc tcatgatcgc
    21421 ctttaaacgg cgtgggtaca gcaatcagcc aggcatccgc ttcaactggc gtcgtgctcg
    21481 ctcgtaaaaa accgccttct acggcagttt ttactacgct cgccaaatca ggctcgacga
    21541 tatggatttc gccacgattg atggtatcaa ccgcgtgttg gttgatatcg acaccaatca
    21601 cctgtttttg ccgtgaggca aacgctgctg ctgtaggcag cccgatgtaa cccagtccga
    21661 taacagaaat ggtcgcaaaa ctcatagtga tatccgatta ttttttaacg cttccagaat
    21721 gcgagagcat gcctgaccat caccatacgg gttatgggcg cggctcatag tttgatattc
    21781 gttttcgtct tttaaaagac gcgtcacttc ctcgacaatt cgctgcttat ccgtgcctac
    21841 cagacgcacc gtacccgccg tcaccgcttc cggacgctca gtggtatcgc gcatcaccag
    21901 caccggtttc cccagcgaag gcgcttcttc ctgaatgccg cctgagtcgg tcaaaatcag
    21961 ccaggcgtgg ttcatcagcc agacaaacgg taaatactcc tggggatcga tcagaatgac
    22021 atttttcaca tgccccagaa tgcgattgac cggctctctg acgttcgggt tgagatgcac
    22081 cggatagaca atctggatgt cctggtgcgt ggtggcaatg tctgccaacg cgtggcagat
    22141 ttcttcaaag ccacgaccga aactttcacg cctgtgaccg gtcaccagaa tcatcttttt
    22201 atcggggtcg ataaacgggt aatttgccgc cagttctgaa cgcagcgtat cgctgctcat
    22261 cacctggtca cgcacccata acagtgcatc aataactgta ttaccagtaa tgaagattcg
    22321 gctatccgca acgttttcac gcagcaagtt ttgccgggaa gtttcggttg gagagaagtg
    22381 atacatcgcc agatgcccgg tcaatgtacg gttagcctct tccggccacg gcgaataaag
    22441 atcgcccgtg cgcagaccag cctcaacgtg accaacagga atacgctgat aaaacgccgc
    22501 caggctggtt gccagcgtcg tcgttgtgtc gccgtgaacc agcacgacgt ctggtttgaa
    22561 ctcggcaaga ataggtttta gcccttccag aatccgacag gttatctctg tcaggccctg
    22621 tcctggctgc attatgttga gatcgtagtc aggtacaatg gaaaagagtt tcagcacctg
    22681 atcgagcatc tcccgatgct gcgcagtgac gcaaacttta gcctcaaaaa aaggatcttt
    22741 tgccaacgca tgcaccaacg gcgccatctt gatggcttcc gggcgcgtac caaatacagt
    22801 cagtactttc acatcgattc tcttcgaata agcggcgagc gcctttgcgc tcaccgcagc
    22861 agtgttgcta tttcgagcaa cggcgggtta atgcgacacc agccccgatc agccccccga
    22921 caatgcccca cataatcatc aggaaggcac gacgtgggct atcgcgtttt accggttctt
    22981 ccggcgtacg caaatagcga taggtctgaa aacgcggatc cagggttgga ccaacattca
    23041 gggtgtttaa catggcccga ttctgatcat agtcgagatc aaaggccgga ccgacggcct
    23101 gtaaattttc cagtcgagcc tggagcattg gacgcccaag caggaacatt tctgaatcag
    23161 gtaattcctc ggcaggcaca tctgtcgcac tgcgcgaaat attatgctgc tcagcaattt
    23221 tcagcgcctg ttcaatactg ttcatccggc ggtcgtagat ggctttcgcc acctcttcct
    23281 gacgcttcac ctgtgctttc atctggatgg tacgcgccgc ccatgcgcct ttcagctcat
    23341 cattcagatg gctggctgca cgctggctgg caaaagcaac atactgacgt aacaggttat
    23401 tagcgtcagg cgcggtttca gcaataagct tcacgctgtc attgaccgcg cgggtaaagt
    23461 ctccggggat aaactggatg ttgttaatca tttcatccag caacgccgca tcggctttgc
    23521 tgttgcccac catccgctgt ttgtaatagt cggtttgcag ccagaactct ctgcgggtat
    23581 cccacgaggc cagttgcata acaaactctt tataggcttc gtccatgacc gatggttggt
    23641 cggcagaagc catgtttgaa cggacatcca ggttacgcaa aaattgctgc tgcgagtaat
    23701 atccccccag catattcacc gttggacgat cggtaatcgc cgtcgagctc cactcctgac
    23761 gagcaaaaaa agtatacgcc agcgcgatta acgcaaatgc cagccccatg ccaataatcc
    23821 atagcttccc agcccacaag gtacgaaaca acccacgaat atccagttca ttttcagcgt
    23881 cttcggccgg tttcccaggc attggttgtg tcatcacatc ctcatttatt tggttaaatt
    23941 ggggctgcca ccacgatttc tacgcagtct gcgttttacg cgcttaataa agcgagcaac
    24001 tttccaggca cgcttaatgc aatatccata gaggaagaat gctagcaaaa agagcaccag
    24061 catgacccac tccgggacaa aatgagaata ttctgccagc acgccaatgg aagcgagcag
    24121 tgccgcagca agggtaatca gcacaaacgc ctgacgggaa gtaaacccgg cacgcatgat
    24181 caaatggtga atatgctgac ggtcaggaga gaatgggctc atgcctttac gcaggcgacg
    24241 gtacataatc gccaccatat ccattagcgg aatggcgatt atccacaaag cggtaaccgg
    24301 gctgatggga tgggttttgc cctgggtcgt ttcgagcagg atccagataa cggtaaaacc
    24361 aatcagcgta ctgcccgcat cacccataaa gactttgtag cggcgaccca ggataccaag
    24421 gttaagcatg atgtatggca ggatggcggc gatcatcgca aagcaccaga ttgcgaggct
    24481 ggtttgcccg tcgaaccaca aaatcatacc gattgctgca aacgagacgc aggacaaccc
    24541 gcccagcaag ccatcaatgc catcaaccat gttgaacgca ttaatggccg cccagacggc
    24601 aaatagcgtc aggaagtaac caaacggtcc gagcaccatc tcccaggagc caaagatata
    24661 acccaggcta ctgagataaa gtttgccgaa caccatcata acaatgccaa cagcggcctg
    24721 tatggtggca cggattttta cgctgatatc aaaacggtca tccagcgcgc caatgaaaac
    24781 aagcacaccg gcacaagcga gatagagaga tgcatgcgga atatagtaat cgacaattcc
    24841 gaacgtgaag caaatccctg cgtaaaccga aatcccccca acgagaggta tcaatccctg
    24901 gtgacgtttg cggaagtttg gtttatccac taaaccgact ttttttgcca ccttacgggc
    24961 aaaaaacaga aacagtgtcg tgaataaaaa aatactgatg agatcagtac tcactgtcag
    25021 taaattcaca atgcatgctc tcagagaaaa ttattagcag aagtataacc acgaagacct
    25081 ttattcagaa gggaaagtcc gttccgaacc caccagtcct gtgcaaaaat taaccatttg
    25141 atatgcaaat tgcataacac gccagcaaat tccaggaatt ttcctaacat cgccagcgcg
    25201 gcattaagat tacagatata aaagcaaaac gccacgtaaa cacgtggcgt ttttggcata
    25261 agacaaattt atgagcgttt catcatttcg aagaaatcgt cattggtctt ggtcatggcc
    25321 agtttattaa tgaggaattc cattgcatcg atttcgccca tcgggtgaat gattttgcgc
    25381 aggatccaca ttttctgcag ttcttcctga gtagtgagca gttcttcttt acgggtaccg
    25441 gaacggttgt agtcgatagc cgggaagacg cgtttttcag cgatcttacg agagaggtgc
    25501 agttccatgt tgcctgtacc tttaaactct tcgtagataa cttcgtccat tttagaaccg
    25561 gtatcgataa gcgccgtcgc gataatagtc aggctgccgc cctcttccac gttacgcgcc
    25621 gcaccaaaga agcgtttcgg acgatgcagg gcgttggcat ccacaccacc ggtcaacact
    25681 ttacctgacg ccggaacaac ggtgttgtaa gcgcgcgcca gacgagtgat ggagtcgagc
    25741 agaatgataa cgtctttctt gtgctcaacc aggcgtttgg ccttctcgat caccatttcc
    25801 gcaacctgaa cgtggcgaga tgcgggttcg tcaaaggtag aagcaacaac ttcacctttt
    25861 accagacgct gcatctcggt tacttcttcc ggacgttcgt cgatcagcag aaccatcagc
    25921 acacaatccg ggtggttgta agcaatgctc tgggcaatgt tctgcagcag catggtttta
    25981 ccggctttcg gcggtgccac aatcagacca cgctgaccac gaccgatagg tgatgccaga
    26041 tccagtacgc gagcagttaa atcttcagta gaaccgttac cacgttccat acgcagacga
    26101 gaatttgcgt gcagcggggt taagttctca aagaggattt tgttgcgggc gttttcaggt
    26161 ttgtcgaagt taacttcgtt aactttcagc agcgcaaaat agcgttcacc ttctttcggc
    26221 gggcgaatct taccagagat ggtatcacca gtgcggaggt tgaaacggcg gatttggcta
    26281 ggggaaacgt agatgtcatc aggaccggcg aggtaggagc tgtctgcgga acggaggaaa
    26341 ccaaatccat cctgcaatat ctccagtacg ccatcaccaa agatatcttc gccactcttt
    26401 gcgtgctgct tcaggatggc aaaaataatg tcctgcttac gcatgcgagc caggttttcc
    26461 agccccatat tttcgccgag agtgatcagc tcagaaaccg gcgtattctt taattcggta
    26521 agattcatag tggtgtgagt tcttaaactt ggggtaaatc tcgaacttaa tgttgtgaat
    26581 ggtatggcag gatcatccat gcctgtttac ggtcatcgac tcatgtcttt tcgctgcctg
    26641 gtcacaggag agtacgcaga actgaaacga caagacggat tgagtgacga gccagaaatc
    26701 tgttcacttc gcatttaaga taaaacggga agcgttggaa attacaagat tcaaacttaa
    26761 taaggtatgt ttaatacgaa gtcaacacta agttagcatg actcacgccg ggcgtccagt
    26821 ttttagcgac ggggcacccg aacatgaaat tcccttacgc caggttagcg tcgaggaact
    26881 ctttcaactg acctttagac agtgcgccca ctttggttgc cgccacttca ccgtttttga
    26941 acagcagcag agtcgggata ccacggatgc catatttcgg cgcagtgcca gggttttgat
    27001 cgatgttcag ttttgcaacg gtcagtttgc cctgatattc gtcagcgatt tcatccagaa
    27061 tcggggcgat cattttgcac ggaccgcacc actctgccca gaaatcgacg aggatcgccc
    27121 cgtccgcttt gagtacatcc gtgtcaaaac tgtcgtcagt caggtgaata attttatcgc
    27181 tcatatataa ctccacagga ataagcctgg cgtgttggtt tcgttgttgg tgtaacatta
    27241 accaactaaa ggttgacttt atttcaccgg atacgctttc gtaaagcaat agtaagctga
    27301 tattctacca cactatgagc aaaacacatt taacagaaca gaagttttcc gacttcgccc
    27361 tgcatccgaa ggttgtagaa gcccttgaaa aaaaagggtt tcataactgt acgcccattc
    27421 aggcactggc ccttccgctg acgctggcgg gtcgtgacgt agccgggcag gcgcaaaccg
    27481 gtaccgggaa aacgatggcg tttcttacgt caacgtttca ttatcttctc tctcatcctg
    27541 cgattgccga tcgcaaggtg aatcaaccgc gtgccttaat tatggcaccg acgcgtgaac
    27601 ttgccgtgca gatccatgcc gacgcagaac cgctggcgga agctactggc ctgaagctgg
    27661 gtctggctta cggtggtgat ggctacgaca aacagctgaa agtgctggaa agcggcgttg
    27721 acattctgat tggcaccacg ggtcgtttaa ttgactacgc caagcagaac cacattaacc
    27781 tcggtgccat tcaggtagtg gttctggatg aagccgatcg catgtacgat ctgggcttta
    27841 ttaaagatat ccgctggctg ttccgccgta tgccgcctgc aaaccagcgc ctcaacatgc
    27901 tgttctccgc cacgctttcg taccgggtac gtgaactggc gttcgagcag atgaacaatg
    27961 ccgaatatat tgaagtggaa ccggaacaga aaacgggcca ccgtattaaa gaagagcttt
    28021 tctacccttc taacgaagaa aaaatgcgtt tgctgcaaac gctgatcgaa gaagagtggc
    28081 cagacagagc gattattttc gccaatacca aacaccgttg tgaagagatc tggggccacc
    28141 tggcggcaga tggtcatcgt gtcggtttgt tgacaggcga tgtcgcgcag aaaaaacgtc
    28201 tgcgtattct tgatgaattt acccgtggcg atctggatat tctggttgcc accgacgttg
    28261 ccgcgcgtgg tttgcatatt ccggcagtga cgcacgtctt taactacgat ttacccgatg
    28321 actgtgaaga ttacgttcac cgtattggtc gtacaggtcg cgcaggcgca agcggtcact
    28381 ctatcagcct ggcgtgtgaa gagtatgcat tgaatttgcc tgctattgaa acctatattg
    28441 gtcactcaat tccggtaagc aaatacaatc cggacgcatt gatgaccgat ctgccaaaac
    28501 cgctgcgcct cacgcgccca cgtacaggca atggtccgcg tcgtactggc gctccgcgta
    28561 atcgtcgtcg ttcaggttaa ataaaatgtc atactcaaaa tcattcaaac tgtatcaaga
    28621 tggcaagaga atgaatcccg atgagcatac tgtagtaagt gattcaggtg aaagaatgca
    28681 gccaacacag agacagattg aaggatgaag agtatgggtt ccacctcgtc gctgtatgca
    28741 gccattgatc tcggttcgaa tagttttcat atgctggttg tgcgcgaggt ggctggaagc
    28801 atccagacgc tgacgcgaat aaaacgcaaa gtgcgtctgg ctgctggcct gaatagcgaa
    28861 aatgccctgt ctaatgaagc aatggagcgc ggttggcaat gtctgcgcct gtttgctgaa
    28921 cgtctgcaag atatccctcc ctcgcaaatt cgcgttgtcg ctacggcgac attacgtctt
    28981 gccgtcaatg cgggtgattt tattgccaat gcgcaggaaa tcctcggttg tccggtacag
    29041 gtgatcagcg gtgaagagga agcacgtctg atttatcagg gcgttgctca cacaactggc
    29101 ggtgccgatc agcgcctggt ggtggatata ggcggtgcca gtactgaact ggtaaccggc
    29161 acgggtgcac aaaccacctc gttgttcagc ctgtcgatgg gctgcgtcac ctggctggaa
    29221 cgctattttg ccgatcgtaa tctggggcag gaaaattttg atgctgcaga aaaagcggca
    29281 cgcgaagtgt tacgtccggt tgccgatgaa ttacggtatc acggctggaa agtgtgcgtt
    29341 ggtgcttccg gcaccgtgca ggcgttacag gaaatcatga tggcacaggg gatggatgaa
    29401 cgcattaccc tggaaaagtt gcagcaattg aaacagcgag ccattcattg cggtcggctg
    29461 gaggaactgg agattgacgg gctgacgctg gaacgtgcgt tagtgttccc gagtggtctg
    29521 gcgatcctga tcgccatttt taccgaactg aatattcagt gtatgaccct ggcgggcggt
    29581 gcgctgcgtg aaggcctggt ctacggcatg ttgcatctgg ccgtcgagca ggatattcgc
    29641 agccgtacgc tgcgtaatat tcagcgccgc tttatgatcg atattgatca ggcgcagcgc
    29701 gtagccaaag ttgcggctaa cttcttcgat caggtggaaa aggaatggca tcttgaagca
    29761 ataagccgcg atttgctcat cagcgcctgt cagcttcatg aaatcggcct gagcgttgac
    29821 tttaaacaag cgccgcaaca cgctgcttat ctggtgcgta atctggatct tcccggtttt
    29881 acccccgcac agaaaaaatt gctggcgacg ctactgctca accagactaa tccggtcgat
    29941 ctctcatcgc tgcatcagca aaatgccgta ccgccgcgcg tcgcagaaca actctgccgt
    30001 ttactccgcc tggccatcat ttttgccagc cgtcgccgtg acgatctcgt gccagagatg
    30061 acattacagg ctaaccatga actgttgacc ttgacgcttc cgcaaggttg gctaacccaa
    30121 catccgctgg gtaaagagat tattgctcag gaaagccagt ggcagagcta tgtccactgg
    30181 ccgctggaag tgcattaatg agtaagtgcc ggatgcgatg ctgacgcatc ttttccggcc
    30241 ttgattattt ccctcgtttt gccgccatca tcgctttcag attcgccaga tggctttgcc
    30301 ctttctgcat ccgttcttcg gcgctgacca ctttgcgctc ctgttcccaa atcagatcat
    30361 cctgcggcag ctccagcaaa aagcggctcg gctccgggcg caccagctcg ccgtactgac
    30421 ggcgttcttt acacagcgta aaggtcaatt ccttctgggc gcgggtaatg ccgacatagg
    30481 ccagccgccg ctcctcatcg atattatctt catcgatgct gctctggtgc ggcaaaaacc
    30541 cttcttccat accgaccatg tagacataag gaaactccag cccttttgac gcgtgcagag
    30601 tcatcagttg cacctgatcc agctcttctt cactctcacc acgctccatc atgtcgcgca
    30661 aagtaaagcg cgtcaccacc tgggtgagcg tcatcggctc atccagttcg ctgccttcca
    30721 gcatctccgt catccagcta aacagttggt tgacgttctt catgcgcatt tcggcggctt
    30781 tcgggctggg cgatgtttcg tacagccagg attcataatc catgccgtgg atcagatcgc
    30841 gcaccgcggc aatcggctcc cgctccgcca gacgctggat ttctgccaac cagtgagtaa
    30901 agcgggtcaa tgcttcataa ccacgtccgc taagcgtctg actcaggccc atatcaaagc
    30961 tggcggtaaa catgctttta ttgcgcgtca tcgcccactc acccagcttt ttcagcgtcg
    31021 ccgggccaat ctctctcttc ggcgtgttaa cgatacgcag aaatgcgctg tcatcgtccg
    31081 ggttagtcag cacgcgcaga taagccagca agtccttgat ttcaggacga gagaaaaacg
    31141 acgtaccacc agaaattttg tacgggatgc ggttttgcat caggaatttt tcaaacactc
    31201 gcgactgatg gttaccgcga taaagaatag cgtaatcttt gtactgcgtt ttattgacga
    31261 agtgatgcgc aatcagttca ccagtgacgc gctcggcttc atgttcttcg ttattcgcgc
    31321 ttaaaacttt aagctctgtg ccgtaaccca gttcggagaa cagacgcttc tcaaagacgt
    31381 gcgggttatt ggcgatcagg atgttcgccg ctttcaaaat acgtccggaa gagcggtagt
    31441 tctgctcaag cttaatcacc ttcagcgccg gaaaatcctg actcagcagc accaggtttt
    31501 gtggacgtgc gccgcgccag gagtagatcg actggtcgtc atcgcccacc acggtaaagc
    31561 gcgcgcggct gcccaccagc agtttcacca gttcatactg gctggtgttg gtgtcctgat
    31621 actcatccac cagcagatag cgaattttgt tctgccagcg ctcgcggact tcttcattgc
    31681 gttgcagcag caacgtcggc aataaaatca gatcatcgaa gtcgagaaca ttgcacgctt
    31741 tcaggtgtgc atcatacagc ccataacaat gggcaaaaat acggtcccgc tcgccaatcg
    31801 cactggctgc cgcctgggac ggtgttttga gatcgttctt ccagttagag atggtcgaaa
    31861 tcaactgttg caggagaact ttgtcatctt caatcagccc ttcggtcaac tctttgagca
    31921 acgccagctg atcggtatcg tcaaacagcg aaaagttcgc cttcatcccc agcgccgcat
    31981 actcacgctt gatgatatcc agccccagcg tgtggaaggt tgagatcatc agcccacgcg
    32041 cctctttgca acccagcgtc tgtccgacac gctctttcat ctcgcgcgcc gccttattgg
    32101 taaaggtcac cgccgcgata tgtcgcgcct gataaccgca accgcggatc agatgggcga
    32161 ttttattggt gatcacacga gttttaccgg aacccgcgcc cgccagcacc aggcaggggc
    32221 cggtaacgaa ttcgacagct tgttgttggc cggggtttag acgcataggt gtattgctca
    32281 atcttcgaac ggggggagga ttgtagcaga atcgcatttg aatgtgaccg cgtgtaagcc
    32341 gctaaccgat tggctgcgga tgtttaaatc agagagggaa gaccgaaacc ggagttcatc
    32401 gtgaatccat tagacttaga aaatatcggg tcgtaacgtt gtgttattac tttttcagag
    32461 tttactaagg agaggtgtac tcgtggcgat taatttcagt cctaaggttg gtgaaatact
    32521 ggattgcaac tttggtaact atcctgttag ccagaatgga cagttcagca ccacgtatta
    32581 cgatggccgc attcctcctg aaatgataaa aaacaggtta gt
//
LOCUS       DAZRUF010000016        32575 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZRUF010000016 DAZRUF010000000
VERSION     DAZRUF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.016"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            209..1582
                     /gene="aroP"
                     /locus_tag="ACHXOL_000644"
     CDS             209..1582
                     /gene="aroP"
                     /locus_tag="ACHXOL_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR6807703.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1625..2479)
                     /gene="ampE"
                     /locus_tag="ACHXOL_000645"
     CDS             complement(1625..2479)
                     /gene="ampE"
                     /locus_tag="ACHXOL_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR6807704.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2476..3027)
                     /gene="ampD"
                     /locus_tag="ACHXOL_000646"
     CDS             complement(2476..3027)
                     /gene="ampD"
                     /locus_tag="ACHXOL_000646"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR6807705.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3115..4008
                     /gene="nadC"
                     /locus_tag="ACHXOL_000647"
     CDS             3115..4008
                     /gene="nadC"
                     /locus_tag="ACHXOL_000647"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR6807706.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4211..4651
                     /gene="ppdD"
                     /locus_tag="ACHXOL_000648"
     CDS             4211..4651
                     /gene="ppdD"
                     /locus_tag="ACHXOL_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR6807707.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4661..6046
                     /gene="gspE"
                     /locus_tag="ACHXOL_000649"
     CDS             4661..6046
                     /gene="gspE"
                     /locus_tag="ACHXOL_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFR6807708.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6036..7238
                     /gene="hofC"
                     /locus_tag="ACHXOL_000650"
     CDS             6036..7238
                     /gene="hofC"
                     /locus_tag="ACHXOL_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR6807709.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPTLLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7273..8316)
                     /gene="guaC"
                     /locus_tag="ACHXOL_000651"
     CDS             complement(7273..8316)
                     /gene="guaC"
                     /locus_tag="ACHXOL_000651"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR6807710.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8472..8516)
                     /locus_tag="ACHXOL_000652"
     CDS             complement(8472..8516)
                     /locus_tag="ACHXOL_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6807711.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8541..9161
                     /gene="coaE"
                     /locus_tag="ACHXOL_000653"
     CDS             8541..9161
                     /gene="coaE"
                     /locus_tag="ACHXOL_000653"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR6807712.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9161..9904
                     /gene="zapD"
                     /locus_tag="ACHXOL_000654"
     CDS             9161..9904
                     /gene="zapD"
                     /locus_tag="ACHXOL_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR6807713.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9914..10111
                     /gene="yacG"
                     /locus_tag="ACHXOL_000655"
     CDS             9914..10111
                     /gene="yacG"
                     /locus_tag="ACHXOL_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR6807714.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10211..10600)
                     /gene="mutT"
                     /locus_tag="ACHXOL_000656"
     CDS             complement(10211..10600)
                     /gene="mutT"
                     /locus_tag="ACHXOL_000656"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR6807715.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10660..13365)
                     /gene="secA"
                     /locus_tag="ACHXOL_000657"
     CDS             complement(10660..13365)
                     /gene="secA"
                     /locus_tag="ACHXOL_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR6807716.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13427..13939)
                     /gene="secM"
                     /locus_tag="ACHXOL_000658"
     CDS             complement(13427..13939)
                     /gene="secM"
                     /locus_tag="ACHXOL_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR6807717.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14170..15087)
                     /gene="lpxC"
                     /locus_tag="ACHXOL_000659"
     CDS             complement(14170..15087)
                     /gene="lpxC"
                     /locus_tag="ACHXOL_000659"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR6807718.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15188..16339)
                     /gene="ftsZ"
                     /locus_tag="ACHXOL_000660"
     CDS             complement(15188..16339)
                     /gene="ftsZ"
                     /locus_tag="ACHXOL_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR6807719.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16400..17662)
                     /gene="ftsA"
                     /locus_tag="ACHXOL_000661"
     CDS             complement(16400..17662)
                     /gene="ftsA"
                     /locus_tag="ACHXOL_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR6807720.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17659..18489)
                     /gene="ftsQ"
                     /locus_tag="ACHXOL_000662"
     CDS             complement(17659..18489)
                     /gene="ftsQ"
                     /locus_tag="ACHXOL_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR6807721.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18491..19411)
                     /gene="ddlB"
                     /locus_tag="ACHXOL_000663"
     CDS             complement(18491..19411)
                     /gene="ddlB"
                     /locus_tag="ACHXOL_000663"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR6807722.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19404..20879)
                     /gene="murC"
                     /locus_tag="ACHXOL_000664"
     CDS             complement(19404..20879)
                     /gene="murC"
                     /locus_tag="ACHXOL_000664"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR6807723.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20933..22000)
                     /gene="murG"
                     /locus_tag="ACHXOL_000665"
     CDS             complement(20933..22000)
                     /gene="murG"
                     /locus_tag="ACHXOL_000665"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR6807724.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21997..23241)
                     /gene="ftsW"
                     /locus_tag="ACHXOL_000666"
     CDS             complement(21997..23241)
                     /gene="ftsW"
                     /locus_tag="ACHXOL_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR6807725.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23241..24557)
                     /gene="murD"
                     /locus_tag="ACHXOL_000667"
     CDS             complement(23241..24557)
                     /gene="murD"
                     /locus_tag="ACHXOL_000667"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR6807726.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24560..25642)
                     /gene="mraY"
                     /locus_tag="ACHXOL_000668"
     CDS             complement(24560..25642)
                     /gene="mraY"
                     /locus_tag="ACHXOL_000668"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR6807727.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25636..26994)
                     /gene="murF"
                     /locus_tag="ACHXOL_000669"
     CDS             complement(25636..26994)
                     /gene="murF"
                     /locus_tag="ACHXOL_000669"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR6807728.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLNAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26991..28484)
                     /gene="murE"
                     /locus_tag="ACHXOL_000670"
     CDS             complement(26991..28484)
                     /gene="murE"
                     /locus_tag="ACHXOL_000670"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR6807729.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28471..30237)
                     /gene="ftsI"
                     /locus_tag="ACHXOL_000671"
     CDS             complement(28471..30237)
                     /gene="ftsI"
                     /locus_tag="ACHXOL_000671"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR6807730.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30253..30618)
                     /gene="ftsL"
                     /locus_tag="ACHXOL_000672"
     CDS             complement(30253..30618)
                     /gene="ftsL"
                     /locus_tag="ACHXOL_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR6807731.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30615..31556)
                     /gene="rsmH"
                     /locus_tag="ACHXOL_000673"
     CDS             complement(30615..31556)
                     /gene="rsmH"
                     /locus_tag="ACHXOL_000673"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR6807732.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31558..32016)
                     /gene="mraZ"
                     /locus_tag="ACHXOL_000674"
     CDS             complement(31558..32016)
                     /gene="mraZ"
                     /locus_tag="ACHXOL_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR6807733.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         7735 a         9152 c         8037 g         7651 t
ORIGIN      
        1 caaacggttt ctttttaagc aactcatctt caaccatgca taaagcgggt gcattcgctg
       61 ccgcatacca ttattcttga tctgacggaa gtctttttgt aacaattcaa acttctttga
      121 tgtaaaaaaa ttaatacaac aaacggaatt gcaaacttac acacgcatca ctgcgtagat
      181 caaaaaaaca atcaccgcac gaggtttcat gatggaaggt caacagcacg gcgagcagct
      241 aaagcgcggc cttaaaaacc gccatattca gcttatcgcg ctgggtggcg cgatagggac
      301 agggctattc ctgggtagcg cctccgtaat acagtccgca gggccaggga ttatcctggg
      361 ttacgccatt gctggtttta tcgcctttct gatcatgcgt cagctgggtg aaatggtggt
      421 cgaagaacct gtcgcaggct cctttagcca ctttgcttat aaatactggg gcagttttgc
      481 cggtttcgcc tctggctgga actactgggt actgtacgtt ttagttgcca tggcagagct
      541 gactgccgtg ggtaaataca ttcagttctg gtatccggaa atcccaacct gggtttctgc
      601 cgccgtattc tttgtggtga ttaacgccat caacctgacc aacgttaaag tgtttggcga
      661 gatggagttc tggtttgcca ttatcaaagt tattgcgata gtagcgatga tcatcttcgg
      721 cggctggctg ctgttcagtg gcaacggcgg cccgcaggcg agcgttagca acctgtggga
      781 tcaaggtggt ttcctgccgc acggcttcac cggactggtg atgatgatgg cgattatcat
      841 gttctcgttc ggtggtctgg aactggtggg gatcaccgca gcagaagctg ataacccgga
      901 gcaaagtatc ccgaaagcga ctaaccaggt tatctaccgc atcctgattt tctatattgg
      961 ttcgttagcc gttctgctct cactgatgcc gtggacccgc gttaccgccg ataccagtcc
     1021 gtttgtgctg atcttccacg agttaggcga tacctttgtg gcgaatgcgc tgaacatcgt
     1081 ggtactgact gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca gccgtatgct
     1141 gtttggtctg gcacaacagg gtaatgcgcc aaaagcgctg gcgtctgtcg ataaacgcgg
     1201 cgtaccggtt aacaccattc tggtgtctgc gctggttaca gcattgtgcg tattgattaa
     1261 ctatcttgct ccggaatccg catttggcct gttaatggca ctggtggtat ccgcactggt
     1321 gatcaactgg gcgatgatca gtctggcgca tatgaagttc cgtcgcgcca agcaggaaca
     1381 aggcgtggta actcgcttcc ctgctctgct ttatccgctg ggtaactgga tctgcctgct
     1441 gtttatggcg gcggtactgg tgattatgct gatgacccca ggaatggcga tttcggtata
     1501 cctgatcccg gtatggctga tcgtgttagg tatcggctat ctgtttaaag agaaaaccgc
     1561 caaagccgta aaagcgcatt aatctctcta cgccctcacc catgcagggt gagggcaata
     1621 atctttacac caacgctccg taaatcgtca gtagcgcaat caccaccacg accacgaacg
     1681 aggttttctt cgccattgaa accgctgcct tcggcgtctc caccttatcg acatgcggtt
     1741 cacgcgccag agagaactgc gccagacgcg ttaacacctg atactgcgaa gtatggaagt
     1801 cacccagcga agcaaaccag gccggtaacg ctttctcccc atgaccgatc aaggcatata
     1861 ccacacccgc aagacgaacc ggcacccaat ccagtacatg aagcacggca tcaatgccgg
     1921 actgtaaacg atgatgcggc gtctggtatc gcgccagcca gtattgccat gcacgcaaaa
     1981 acgcataccc catcagcgta acgggtcccc aggttccccc cacaatcaac cagaacagcg
     2041 gtgcaagata aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca cgcaaatact
     2101 cacgttcgtc gcagcctgcc gggacgccgt gaatcatggt gagttcgcca gccatcgtgg
     2161 cacgggcatg gctatcatta cgtgaagcag ctgtcagata agcatgataa tgaagacgaa
     2221 ctttacctgc gccaatacac agcaaaccaa tcagcagcca caccagcagc gtgggaacgt
     2281 tgaacaatac tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc gcaataatgg
     2341 tcatgcctaa cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac gcttcaagac
     2401 gatgatcaag ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt aacaccagta
     2461 aggttgtaaa tagcgtcatg ttgtctcctt gctgaccagc gcacgaaacc gtgcccaatc
     2521 aaatgcagga ccgggatcgg ttttccgatc cggcgcaata tcacaatggc ccgtcatgtt
     2581 attagcgata tcgggatagc gatctatcag tgcccgcgta accgccgcaa gctgttgata
     2641 ctgcgcatcg gtatacgcca gagtatcggt gccttcaagc tcaatcccaa tagaaaaatc
     2701 attgcagcgt tcacgcccct gatactgaga gactcccgca tgccaagcac gtttatcgaa
     2761 aggaacatac tggactattt caccatcacg gcgaatcaaa cagtgagcgg agacgcgcaa
     2821 atgggcgatc tcagcaaaga aaggatgtgc ctgcggatca atagttccag tgaataatgc
     2881 gtcgatccac ggaccgccaa actcgcctgg cggcaggcta atattgtgca ccaccagcag
     2941 ggtgggtgtt tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc ggcgcgcgcc
     3001 aaccagccac ccctgttcta acaacatgca ggatctcctt atatgtggtg ctaatacccg
     3061 gttcagagta gcatgtttct accttatgat tcgttagcta tctggagttt taacatgccg
     3121 cctcgccgct ataaccctga cacccgacgt gacgagctgc tggaacgcat taatctcgat
     3181 atccccggcg cggtggccca ggcgctgcgg gaagatttag gcggaacagt cgatgccaac
     3241 aatgacatta cggcaaaact tttaccggaa aactctcgct ctcatgccac ggtgagcacc
     3301 cgcgagaatg gcgtattttg cggcaaacgc tgggttgaag aggtgtttat tcaactggca
     3361 ggcgacgatg tcaccataat ctggcatgtg gatgacggcg atgtcatcaa cgccaatcaa
     3421 cccttgttcg aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg cactgcgctt
     3481 aattttgtgc aaaccctttc aggagttgcc agtaaggtac gccactatgt cgaattactg
     3541 gaaggcacca acacgcagtt gttggatacg cgcaaaacct tacctggcct gcgttcagct
     3601 ctgaaatacg cggtactttg cggcggcgga gcgaatcacc gtctggggct ttctgatgcc
     3661 ttcctgatca aagaaaatca tattattgcc tccggctcag tgcgccaggc cgtcgaaaaa
     3721 gcgtcctggc tgcacccgga tgcgccagta gaagtcgaag tagagaatct ggaagaactt
     3781 gatgaagccc tgaaagcagg agccgatatc atcatgctgg ataacttcga aacagaacag
     3841 atgcgcgaag ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc tggcaacgtc
     3901 actgacaaaa cactgcgcga atttgccgaa acgggcgtgg actttatctc cgtcggtgcg
     3961 ctaactaaac acgtacaagc actcgacctt tcaatgcgtt ttcgctaatt gctgacagat
     4021 ttatccgctc catcgcgggc ggataatctc cccttcccca actttcttcg taacgcctcg
     4081 caaatttctt gtcttttgct gcaacgaaat aaaaaacctc ggaagctgct ttcccgtttc
     4141 gttttttgcc cctcgcttgt gctgatggcg tttgccaaag tagcgccaac caaatcaagg
     4201 agcgaaacag atggacaagc aacgcggttt tacacttatc gaactgatgg tggttattgg
     4261 catcattgcc attttaagcg ccattggtat tcccgcttat caaaactacc tgcgcaaagc
     4321 tgcactcacc gacatgctac aaacctttgt gccttaccgt acagccgtag agttgtgcgc
     4381 gctggaacat ggtggattag atacctgcga cggtggcagc aatggcattc cctcgcctac
     4441 caccacccgc tatgtttcag ccatgagtgt ggcaaaaggc gtggtgtcgc tgaccgggca
     4501 agaaagtctc aatggactaa gcgtcgtcat gacaccgggt tgggataacg caaacggcgt
     4561 caccggctgg gcgcgcaact gcaatattca aagtgacagc gcattgcagc aagcctgcga
     4621 agatgtcttc cgctttgatg acgccaacta aggagcggca atgaatattc cacagctcac
     4681 cgccctgtgt ctgcgttatc agggagtctt gctggatgcc agcgaagagg tggttcatgt
     4741 tgcggtagtc gatgcacctt cgcatgagtt gctggacgca ttgcatttcg ctaccaccaa
     4801 acgtattgag atcacctgct ggacgcgcca acaaatggaa ggccacgcca gtcgcacaca
     4861 acagacattg cccgtagctg ttcaggagaa gcatcagccc aaagcagagt tgctgactcg
     4921 aacgttacaa tctgcgctgg aacaacgtgc gtctgatatt catatcgaac cagcggacaa
     4981 tgcctaccgc atccgcttgc gtatcgacgg cgtattgcat cctttaccgg atgtttcacc
     5041 ggatgccgga gtcgcattaa ccgccagatt aaaagtgctg ggaaacctgg atattgcgga
     5101 acatcgcctg ccgcaggacg ggcaattcac tgtcgaactg gcaggaaacg ccgtctcatt
     5161 tcgtattgcg accttaccat gtcggggtgg tgaaaaggtg gtattaaggt tgttacagca
     5221 ggtgaaccag gcactggatg ttaacacgct gggaatgcag ccgttacaac tggcggactt
     5281 tgctcatgcc ttgcaacaac cacagggact ggtgctggta actggcccta caggcagcgg
     5341 caaaacggtc acgctttata gtgccctgca aaagctgaat accgctgaca ttaatatttg
     5401 tagcgtcgaa gatccggttg agatccccat agccggacta aaccagacgc aaatccatcc
     5461 gcgtgccggg ctcacctttc agggcgtttt gcgtgcgtta ttgcgccagg atcctgacgt
     5521 catcatgatc ggagagatcc gcgatggcga aacggcagaa attgccatta aagccgcgca
     5581 aaccggtcac ctggtgttgt ctaccctaca cactaattcc acctgcgaaa cgctggtacg
     5641 tttacagcaa atgggagtcg cccgctggat gctctcatca gcgcttacgc tggtaatagc
     5701 ccagcgtctg gtacgtaaac tttgcccaca ttgtcgccag cagcaagggg agcccatcca
     5761 tattccagtc aatgtatggc cgtcgccgct gccccactgg caggcacccg gttgtgtaca
     5821 ttgctaccac ggtttttatg gtcgtacggc cttatttgaa gttctgccca taacgccggt
     5881 cattcgtcag cttatttccg ctaataccga cgttgaatcg ctggaaacgc acgcacgaca
     5941 ggcgggtatg cgtacgcttt ttgaaaacgg ctgcctggcc gtggagcaag gcttaaccac
     6001 ctttgaagag ttaatccgcg tactggggat gccgcatggc gagtaagcaa ctctggcgct
     6061 ggcatggcat aaccggcgac ggcaatgcgc aagatgggat gctatgggca gagagccgtg
     6121 ctttgctgct catggcacta cagcaacaga tggttacccc acttagcctg aagcgaatcg
     6181 ccatcaattc tgcgcagtgg cgaggagata aaagcgcgga agtcattcat caactggcga
     6241 cgctactcaa agccgggtta acgctttctg aagggctggc actgctggcg gaacagcatc
     6301 ccagtaagca atggcaagcg ttgctgcaat cgctggcgca cgatctcgaa cagggcattg
     6361 ctttttccaa tgccttatta ccctggtcag aggtatttcc gccactctat caggcgatga
     6421 tccgcacggg tgaactgacc ggtaagctgg atgaatgctg ctttgaactg gcgcgtcagc
     6481 aaaaagccca gcgtcagttg accgacaaag tgaaatcagc gttacgttat cccatcatca
     6541 ttttagcgat ggcaatcatg gtggttgtgg caatgctgca ttttgttctg ccggagtttg
     6601 ccgctatcta taagaccttc aacaccccac taccggcact aacgcagggg atcatgacgc
     6661 tggcagactt tagtggcgaa tggagctggc tgctggtgtt gttcggcttt ctgctggcga
     6721 tagccaataa gttgctgatg cgccgaccga cctggcttat agtgcggcag aaattgctgt
     6781 tacgcatccc gattatgggt tcactgatgc ggggacaaaa actcacgcag atctttacga
     6841 ttctggcgct gacacaaagt gcaggcatta cttttttgca gggcgtagag agcgtcagag
     6901 aaacaatgcg ctgcccgtac tgggtgcaac ttctgacaca aatccagcac gatatcagta
     6961 acggtcatcc catctggctg gcgctaaaaa ataccgggga gtttagcccg ctctgtttgc
     7021 aattagtgag aacaggagag gcatccggct cgctggacct catgttagac aacctcgccc
     7081 atcatcatcg ggataacaca atggcgctgg cggataacct cgcagcctta ctggaaccga
     7141 cgttgctgat cataacggga ggaattatcg gtacgctggt ggtggcgatg tatctgccaa
     7201 ttttccattt aggcgatgcg atgagtggga tgggataacg ctggcgtgtt gctccacacc
     7261 agcgttggaa gattacaggt tgttgaagat gcggttttct tgttcctgca cacggataaa
     7321 tgtggtgcgc ttggtcagtt ctttcaggcg tgaagcccca acgtatgtac aagctgaacg
     7381 caggccgccc aaaatatcac gcgcggtatt ttcaaccggg cctcgcagcg gcagcttaac
     7441 ggttttacct tctgctgcgc gatattccgc aacgccgcca acgtgacgtt tcatcgcaga
     7501 ctcggagctc atgccgtaga acagcataaa tttctcgccg ttctcctcaa cgatgcgacc
     7561 gccgctctct tcgtggcccg ccagcatgcc gccaagcatg acgaaatcgg caccgccgcc
     7621 aaaggctttt gcgacatcgc ccggcgtggt gcagccaccg tcgctgacga tcattccgcc
     7681 cagaccgtgc gccgcatcgg cacattcgat taccgcagaa agctgcggat aaccgacgcc
     7741 tgttttgacg cgagttgtac aaacagaacc tgggccaatg ccaactttaa cgatatcagc
     7801 accggagagg ataagctcct cacacatttc accagtcact acgttgccag cacaaatggt
     7861 tttggtcggc cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt cggaataacc
     7921 attcgccaca tcaatacaaa cgaagtttaa tgccgggttc aggtcgagaa tctgtttagt
     7981 tttttcgaaa tccgcatcag acgtaccggt agaaaccatc acatgtttca gcacatcagc
     8041 ggaagaattg ttgataaacg cttgccactc ttcgacagaa tagtgtttat gcacagcagt
     8101 caaaatatca aaagaagcca gcgcagaggc catagaaaat gtacctacgg tgtccatatt
     8161 tgcggcgata atcggcacgc cggaccagct ctgacctgaa tgtttgaagg tgaattgacg
     8221 ttccagttca acatcggaac ggcttttaag agtggagcgt ttagggcgga tgagaacgtc
     8281 tttaaaacct aacttcagat cttcttcaat acgcatgtgc ggattcctgg ggttaatggc
     8341 tataagggta agagcgcaac tccagtgacg ctatcatacg cactaatcaa tgcggcgcaa
     8401 gactgcgaaa ttgtcttttt ttacgctaca atcccataaa tttacctggt aaatagtgag
     8461 cgaacgtttc atcaatttcg ctttcgtgca ctcattttta acgttttgat aatcatgatg
     8521 aattcccggg aatataaatt atgaggtata tagttgcctt aacgggaggc attggcagtg
     8581 gcaagagtac cgttgccaat gcgtttgctg atctcggaat taacgtcatt gatgccgata
     8641 ttattgcgcg tcaggtggtt gaaccaggtg cacctgcgct acatgccatt gctgatcact
     8701 ttggcgctaa catgattgct gctgatggaa cattgcagcg ccgggccttg cgcgagcgga
     8761 tcttcgccaa cccggaagag aaaaactggc ttaacgccct gctgcatccg ctgattcagc
     8821 aagagacgca acaccagatc cagcaagcta cttcccccta tgtactgtgg gttgtgccat
     8881 tgctggtaga aaactcactg tataaaaaag cgaatcgagt gctggtggtg gatgtcagcc
     8941 cagaaacgca acttaagcgc accatgcagc gcgatgatgt aactcgcgag catgtcgaac
     9001 aaatccttgc tgctcaggca acgcgcgaag cccgccttgc cgtggcagat gacgtcattg
     9061 ataataacgg cgcaccggat gctatcgcat cggatgttgc ccgcctgcac gcacactatt
     9121 tgcagcttgc gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc aggtcctttt
     9181 tgaacatcca ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt tgattcagca
     9241 actcaccgtt aatttaccca tcgttgacca cgctggcgcg ctgcatttct tccgtaatgt
     9301 cagtgaatta ctggatgttt tcgagcgcgg cgaagtccgc actgagctgt tgaaagaact
     9361 tgaccggcag caacgtaaac tccagacctg gattggcgtg cctggcgtgg accagagccg
     9421 tattgaagca ttaattcagc agttaaaagc ggcggggagc gtattaattt ccgcgccgcg
     9481 tatcgggcaa tttctgcgtg aagatcgttt gattgctctg gtgcgtcagc gactgagcat
     9541 cccaggcggc tgttgcagct ttgatttacc tacattgcac atttggctgc atctacccca
     9601 ggcgcagcgc gacagccagg tagaaacctg gattgccagc ctgaacccgc tcacccaggc
     9661 acttaccatg gtgctggatt taattcgcca gtcggccccc ttccgtaaac aaaccagtct
     9721 gaatggtttt tatcaggata acggtggcga tgccgacttg ctgcgcctga atctgtcgct
     9781 cgattcacag ctttatccgc aaatttccgg acataagagc cgttttgcca ttcgttttat
     9841 gccgctggac actgaaaacg gacaggtacc ggaacgtctg gatttcgaac tggcctgttg
     9901 ctaaggagta aaaatgtcag aaactattac ggtgaattgc ccaacctgcg ggaaaacggt
     9961 ggtgtggggt gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc agctgatcga
    10021 cctcggagaa tgggctgctg aagaaaaacg aatccccagc agtggcgatc tttccgaaag
    10081 cgatgactgg agcgaagagc caaagcagtg acatttgtct gatgccgcac gtaggcctga
    10141 taagacgcgg acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg gcgtgaacgc
    10201 cttatccgac ctacagacgc ttaagcttcg caattaccgg ttcattggct ggcggaaaat
    10261 catcggcatt aagaccgact agcgacatcc actcaccggg ttgcccttct ttaccccacg
    10321 gctccccttc ccagctttcg accagccaga accacagagt tatatgcctg tccgggaatt
    10381 catattccag tttttcaaat agcgaaaaat gttgtggggt aatcccgact tcttcctgaa
    10441 gttcacgcac taccgcctgt tccggcgttt cgcccatttc aattttaccg ccgggaaact
    10501 ccagtttatt cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt tcattgttct
    10561 cgttgcgaat aatacctacc gcaatttgca gctttttcat tgtcttaaac ctataaaaaa
    10621 ggcgcagaat cctgcgcctt ttacttcaac tgttagcttt tattgcaggc gaccatggca
    10681 ctgcttgtat tttttaccag aaccgcacgg gcaaggatcg ttacgtccta ctttgcgctc
    10741 accggtttgc gccgccagtg cagctgcggc tgcagagtcg tcatcctgat ggctaagctg
    10801 ctgcatttgc gctaaacgct cggcttccat acgacgctgt tgttccagct cctcaacctc
    10861 ttcaggcata cgtacctgga ctttgctcag cgtgctgata acttcatatt ttaacgactc
    10921 cagcatcgct gcaaacatgg agaacgattc acgtttgtat tcctgcttcg gatctttctg
    10981 tgcatagcca cgcaggtgga taccctgacg cagatagtcc atcgccgcta ggtgctcttt
    11041 ccacagagag tcgagagttt gcagcatgac gcctttttcg aagtgacgca tcatctcagc
    11101 accaaccact tcttctttac gctgatacac ttcgatggac tgcgccagaa ttcgctcacg
    11161 cagcgtctct tcatgcagtt ctggttcttt atccagccac tcggcaattg gcaaatcgag
    11221 gtcgaaatcg ttcttcagac gttcctgcag ccccggaata tcccacattt cttccagtga
    11281 ctgtggcgga atgtaggcat caatggtcgc tttgaacaca tcttcacgaa tgctgttgat
    11341 ggtttcgctc acatcgctga catccagcag ttcgttacgc tgggagtaaa tggcgcgacg
    11401 ctgatcgtta gccacgtcat catattccag cagttgctta cgaatgtcga agttacggct
    11461 ttcaacttta cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt caatggcttc
    11521 gcctggcttc atacccagtt tacgcatcat gccggatact cggtcggaag caaaaatacg
    11581 catcagcgca tcttccatcg acaggtagaa acgggaagaa ccagcatccc cctgacgacc
    11641 agaacgaccg cgcaactggt tatcgatacg acgggattcg tgacgctcgg taccgatgat
    11701 atgcaggcca cctgcttcca gtaccgcatc gtgacgtacc tgccagtcgg ctttaatttt
    11761 ttcaatttgc tctgcggtcg gattttccag cgcggcaact tctgcctgcc agctaccacc
    11821 gagcacaata tctgtaccac gacccgccat attggtcgcg atagtcaccg cagccggata
    11881 acctgcctga gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca ggacgttgtg
    11941 cttaataccg gctttggtca gttcgtttga caccagctcc gatttttcga tggagatagt
    12001 acccaccagc accggctggc ctttcgcagt acgttctttg atatcttcaa tgatcgcctg
    12061 aattttttcc gcttcagtca tgtagaccag gtccggcaga tctttacgaa tcattggacg
    12121 gttggtcgga acaacgacgg tatccagctt gtagatggag ctaaattcga aagcttcggt
    12181 atcagcagta ccggtcatcc ccgccagttt ttcatacaga cggaagtagt tctggaaggt
    12241 gatcgaagcc agcgtttggt tttcgttctg gatctgcaca ccttctttcg cttccacagc
    12301 ctggtgcaga ccatcggacc agcgacggcc ctgcatggta cgaccggtgt gttcgtcaac
    12361 gatgataact tcaccatctt taacgatgta gtcgacgtcg cgggtaaaca gcgcatgagc
    12421 gcgcagcgcc gccgttacgt ggtgcatcag catgatgttg gccggagagt acagagactc
    12481 cccttcatcc atgatgccct ctttcaccag cagttcttca atcagcacca gaccacgttc
    12541 ggtcaggttc acctggcgag atttttcgtc caccgagaag tggccttcgc cctggaaggt
    12601 ttcggagtct tctttttcct gacggatcag gtgcggaata attttattca cgcgtttata
    12661 catttccgag ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg cttcatcgat
    12721 caggatggag tccacttcgt ccaccagcgc atagtgcagt ttacgctgta cacgttcttc
    12781 agggctgaac gccatattgt cgcgcaggta gtcaaagccg tattcgttgt tcgtaccgta
    12841 agtgatgtca gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg gcaggttgat
    12901 accgacagtc aggccaagga attcaaacag cggacggttg ttttcggcgt cacgttgcgc
    12961 caggtagtcg ttgacggtaa ctacgtgcac gcctttaccg gttagtgcgt tcaggtaagc
    13021 aggcagcgtt gcggtcaggg ttttaccttc accggtacgc atttcggcga tgcagcgttc
    13081 gttaagaacc ataccgccga gtaactgaac gtcgaagtga cgcataccaa agacgcgctt
    13141 acttgcctca cgtaccacgg cgaaagcttc cgggatcaga ttttccagca cttcgccttt
    13201 ttccagacgt gcacgaaact ctgcggtttt ccctttcagt tcttcgtcgg agagtttttc
    13261 catctccggt tccatggcat tgatgatgtt gaccactttg cgcatccggc gcagggtgcg
    13321 atcgttacga ctaccgaaaa ctttagttaa caatttgatt agcataataa aatctcaaac
    13381 gccccgcgtt gcggagttaa taaaatgaag taaaggttta ttgttgttag gtgaggcgtt
    13441 gagggccagc acggatgcct tgcgcctggc ttatccagac gggcgtgctg aattttgctt
    13501 gtggggtaaa atgcgcataa tcaatgcgat aacccttttc agacggcgtg ccttcctggg
    13561 tcagcagcgc gctgagcgta tccagtaatg caagatgttg cgcctgaaga ggcaaagatt
    13621 cttcagcaac gggcagtgtt tgcggtgcca ttgcgaaaga aagatgacgg attaccgtgc
    13681 gaatggcatg ttgatgccag taatcaacgg aatagttcga attcgggcgg cgtgtgttcg
    13741 cttccagcaa ggccaattga ccaaagttaa ctttggctga aggctcgtgg ttgcgggttg
    13801 tcgcttttgc gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa cctaaactcg
    13861 ccgcaaccat ccctaataag agatgcggcc agaagtagcg tttaccaaac tgtcgccagc
    13921 gcgtcagtat tccactcacg ttattgccat cccgatcgta ttcaaacgta tttagcgcac
    13981 aaatcttatc attaaagcct gcagtccaca gcacgaatga agataagttg tgcggaaaag
    14041 tgcttaagaa aacagcgttc gcaccgtttt ttatagctct tataaaattc aaaaaaaaat
    14101 gactggttca cctggccgga gagagtgcca gatttgccag tcgaatttta tacgacagta
    14161 taaatgtcgt tatgccagta cagctgaagg cgctttgaag gccaacggca gttctgcgtc
    14221 gtcctggaag gtcacatatt cccaggcttc ctgtttcgcc aggacagcct gcagcagttt
    14281 gttattcagt gcatgaccgg atttataagc ggtaaatgca ccaataatat tgtgaccaca
    14341 catgaacaag tcaccgatcg catcgagcat tttgtgacgc acaaattcgt cttcaaaacg
    14401 caggccgtct tcgttcagta cgcgataatc gtcaacaacg atggcacaat cgaagctgcc
    14461 gcccaggcac aaaccacggg actgcagata ttcgatatca cgcatgaaac cgaacgtacg
    14521 cgcacggctg atctggcgca taaacgcatc agcggagaag ttcatcgcat agcgctggtt
    14581 gctggaatca atagccggat ggttaaaatc gatggtgaaa tccagcgaaa aaccattgta
    14641 cggcttaaat tcagcccact tatcgccatc ttcgacacga acagtctctt tgatgcgaac
    14701 aaattttttg gcgcagttca actcgtcgat accggcgtca agcagcaggt atacaaacgg
    14761 agcggcgctg ccgtccatga tcgggatttc cggcgcgtta acttcgataa caatgttatc
    14821 gatgcccagg cccgcgagag cagcattgag gtgctctacg gttgaaatcc gtacatcatg
    14881 ctcgttgacc agacacgtac agagcatggt atcacgcaca gatttggcat cggccgggaa
    14941 atctaccggt ggattcaagt cggtgcgacg atagatgacc ccggtgttgg ccggcgcagg
    15001 gcgtaacgtc agagtgactt tcttgccggt atgtaaaccg acacccgtcg cctgaacgat
    15061 acgtttaagt gtcctttgtt tgatcatcgt attatctcgc caaattacct atccaaccga
    15121 agtgtactat acattcggcg ggccagttta gcacaaagag cctcgaaacc caaattccag
    15181 tcaattctta atcagcttgc ttacgcagga atgctgggat atccagataa tccggctctt
    15241 tcgcagtttg cggcgcattg tcattcacga ctttagcaac cggcttctgc tcctgggtca
    15301 gcggagccat cccatgctgc tggtagcgat ccatcactgg ctgctgaacc tgcttattgg
    15361 tcaccagagt gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca acggttacgc
    15421 gcagctcgtc attcatatcc gggtcaagag aagtaccgat aaccacagtc gcgttgtcgg
    15481 aagcaaatgc acggatggtg ttacctaccg tttcgaactc atccagacgc aggtcgaagc
    15541 ccgccgtgat gttaaccagc acgccgcgcg cgccagacag gtcgatatct tccagcagcg
    15601 gagaagagat agccatttca gcagcttctt ccgcacggtc ttcaccgctc gccacgccag
    15661 aacccatcat tgcgtagccc atctcagaca ttacggtgcg tacgtctgca aagtccacgt
    15721 tcatcaaacc cggacgagta atcagttcag cgataccttg cacagcgcct ttcagtacat
    15781 cgttcgctgc gccaaacgca tccagcaggg agataccgcg gcccagaact ttcagcagtt
    15841 tgtcgttcgg gatagtgatc agagagtcca catgcttgga cagttcagtg atcccctgct
    15901 ccgcgaatgc catacgcttc ttgccttcaa agttgaaagg cttagtgacg acagcaacgg
    15961 tcaggatacc caaatctttt gccacttcag cgacgactgg tgctgcacct gtaccggtac
    16021 caccacccat acccgcagca ataaagacca tgtctgcacc ttccagcgcc gcacgcaatg
    16081 catcgcgatc ctcatcagcc gcattgcggc caacttctgg attagcgcca gcgcccagtc
    16141 ctttggtgat accgctaccg atttgaatcg tctgtccaac cgctgtttta cgcagcgctt
    16201 gtgcatcggt atttaccgcg aagaattcaa caccttcaat acgctcgcgc accatgtgtt
    16261 caacagcatt accgccgccg ccgccgacgc cgatgacttt aatcaccgcg tcattggtaa
    16321 gttccattgg ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga ggccgtaatc
    16381 atcgtcggcc tcataaaaat taaaactctt ttcgcagcca actattgagt cgcttgatcc
    16441 acgagccaac tgatgctgta acacgttttt ctacttcagc ttcaccgtta agatgtgact
    16501 ctttcccata gtgaagcaat cccaccgccg tcgaataata cggctcctga gcataatccg
    16561 ttaaaccggt aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac acgcgctgag
    16621 cacaggctgc aagaccttcg atctgcgctg cgccaccggt taatacaatg cctgccgcca
    16681 ggtgatgttt aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc tcttcgttga
    16741 ccaggttgag cagctcggta tagcgcggct cgatcacctc tgccagggtc tgacgttgca
    16801 gactccgtgg cggacgaccg cctacgctcg gcacttccac gctctcatct tttccaacga
    16861 tggaacccag cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt ggcggcgtgc
    16921 caaaggcgta agcgatatca ctggtcacga cattgccagc ataaggaatt accttagtgt
    16981 ggcgcaatgc cccaccggta taaacggcga tatccattgt accaccaccg atatcgacga
    17041 cgcagacacc cagttcacgt tcatcttccg tcaataccga ataacttgat gccagtccgg
    17101 caaatatcag ttggtcaact ttcagcccac aacgttcaac cgctttgacg atgtttttcg
    17161 ccatatcgtt gtgacatgtg atcaggtgca cttttgcctg catccgcacg cccgaaagtc
    17221 ctaccggatt cttgatccct tcctgatagt caatcgcata ctcttgcggg atcacatgca
    17281 gcacacgatg ttcatcgcgc acacgcaccg atttcgcggt atggacgacg ttttccacat
    17341 cttcttgcgt cacttcttct tcagaaatag gcaccatacc aatttcattc tggcagctga
    17401 tgtgcttacc agaaagcgcc agatataccg aagagatctg acaatctgcc atcaattctg
    17461 cctggtcaat ggcgcgttgt acgcacttga ccacggattc gaggtcgttc accccgcctt
    17521 tatccatacc acgtgacggg cagctgccca cgccaatgat attgaccata ccgtcgggca
    17581 gaacttcccc tactaaagcg gcaaccttcg cggtaccaat ctccagtcct actaccagtt
    17641 ttctgtccgt cgccttgatc attgttgttc tgcctgtgcc tgattttgtt gctgagtaga
    17701 ttcctctggc ggcaagggcg cccagcctac tgccgctcca gagtcataac gcaaatcaac
    17761 gtagctaatc cgtttgccat cggtttgcgc ctgctgctgt aaaaccggat aaagttctac
    17821 aaagcgagcc aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa tatcgttatt
    17881 cagcgtcaac tgccaggaac gccgcgcggt catcgccgct tccttcagag taaatctgtc
    17941 ctttgccagc atctgcccca tttcgcgata gccctgcaac acttcattgg cgctgccttc
    18001 cgggccatac agcattggaa gcacctgctt gctggtgcga tctggcggca cgctgaaggt
    18061 gtttccttcc gcgtctacca tatgttgatc attccaccgc gcaatcggca catattcaac
    18121 cagatgaatc ttcaattcat caggccactg ctttctgacg ctcacctgct taatccacgg
    18181 caggcgttgt tctatttgcg tctggatgat gttgacatcc tgggtcataa aggtacccgg
    18241 ctcacccaat gccaggatcg actgccggat atcgtcatta cgcgtgtaat ggcgttcacc
    18301 ggtcaacacc agctttgaga gcggcaggcg ttgcgcatct tccatccagc ccaacacgac
    18361 ccagccgctc accaacactg tcgttaaaac ggtcagcagg aaaaggatcc ccgccagacg
    18421 cgttccatta ttgcggcgag aagaaacctc ttcttcgcta tttcgcgtgt tcagagcagc
    18481 ctgcgacata ttagtccgcc agttccagaa ttcgtactac caactgcgag aagctcatac
    18541 ctgcctgacg tgccgccatc ggcaccaggc tgtggctggt catacccggt gaggtattgg
    18601 cttccagcag ataaaactgt ccatcgctgt ccagcataac gtcaatacgt ccccatcctt
    18661 tgcaacctaa cgtcgtccat gctttcagca ctaatgcctg caaattggcc tcttgtgacg
    18721 cttccagacc tgcggggcag aaatactgtg tctcatcaga gagatacttc gcctcataat
    18781 catagaaggt tccggacggt tgaatacgta ttgacggtaa aatttcttca ccgagtatcg
    18841 caaccgtgaa ctccggccca cttagccatt tttcaatcaa tacttcttca tcgtgctgaa
    18901 atgccaatct taatgcatct tgtagagcat tttctgctac tacttttgac attcccacac
    18961 tggaaccttc gcggctcggc ttaacgataa ccggcaaacc cagagcagaa atttctgcta
    19021 actgcttatc gctcaggcct ttttcaaact ctgcgcgggt taacgctacc cacggcgcga
    19081 ccggtaaacc ggcaccttgc cacagcagtt tgctgcgtag tttatccatt gaaagcgcag
    19141 atgccatcac tccgctgccg gtataaggca agcccatcag ctcaagcatg ccctgcagcg
    19201 taccatcttc accgccgcga ccgtgtagcg cgataaacac tttctgaaaa cccatcgact
    19261 tcagttgcgt cacgtcgact tctttcgggt cgacaggata cgcatcaata ccgccttcac
    19321 gcagcccggc taacaccgct gcgccagaat tcagagaaac ttcccgctca gcggaagtcc
    19381 cgcccaacag gaccgcaatt ttatcagtca tgttgttctt cctccggagt ttgcggcttc
    19441 agtttgattt cagctaaaga acgggcaatt tttccgatat taccagcccc ctgaacgaga
    19501 atcaggtcgt taccgattaa taccggtgcc agcatctcgg ctacctgcgc agggtccggc
    19561 accagaatgg gatcaatttt cccacgtcca cgaattgtgc gacacagcga acggctgtcc
    19621 gctcccggaa ttggcgcttc gccagccgga tacacttcca gcatcaacag ggtatcaacc
    19681 tgcgtcagca cattggcgaa atcatcatac aggtcgcgcg tacgggtaaa acggtgcggc
    19741 tgaaacagca ttactaggtt tttatccggc cagcctgcgc gcgccgcttt aatggtggcg
    19801 tcaacttccg tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc gcttttacca
    19861 ttcactggct ccagcgggaa ttcaccgagg aaatcaaaac ggcgaccagt cccctggaag
    19921 ctctccagcg cacgcaaaat agcctcgtcg tcaattccct cttccgtagc aaccgcaacc
    19981 gcagctgcgg cgttcagcgc gttatgacgg cctggcgcat tcagggtgac gcgcatcggc
    20041 tctttgtcct ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg ataatcttct
    20101 acacgcacgt cggcatcttc gctgaagccg taagtcgtgg tctgacgtcc cacacgcggt
    20161 aacaattcgc ggatcaccgg atcatcaaca cacatcaccg cacgaccgta aaacggcagg
    20221 ttgtgcagaa aattaataaa agtctgtttt aaattctcaa agtcgccctg gtaggtatcc
    20281 atgtggtcgg cttcgatatt ggtgacaatc gccaccatcg gttgcagatg caggaacgat
    20341 gcatcactct catctgcttc ggcaatcagg taccgaccat gccccaaacg cgcatgaacc
    20401 cccgccgctt ttaccagccc gccgttaacg aaggttgggt cgagccccgc ttctgcgtag
    20461 atgctggaaa ccatcgcggt ggttgtcgtt ttgccgtgcg ttcctgcaat ggcgatgcca
    20521 tgacgaaaac gcattaactc agccagcatt tcggcacgac ggatcaccgg aatacgcgct
    20581 tcatgagctg cgacaatttc cgggttatcg gcagaaatgg cgctggaaac aacgaccacg
    20641 ctggcatcac gtacgttttc cgggcgatgg ttgaaataaa tcgtcgctcc cagattcatt
    20701 aactgctgcg tgaccggatt tggcgctaaa tcggaaccac tgatctgata accttcattg
    20761 gccagaactt cggcaatacc gcccataccg gcaccaccga tgccgacaaa atgtatgtgc
    20821 cgaacgcgac gcatttcggg cacgatggaa cgcagttttg ccaattgttg tgtattcatt
    20881 ctttacgcca ttaacttctt aaattcatac gatgcaaaag gcatcgctac aattacgccc
    20941 gggcagcccg gctcacttca tttgccactc gctcggtggc atccggaatg gatgcagcgc
    21001 gggcgcgttc tgccatggtt aataaggttt ctcgcgacca cccggccagg gtgttggcga
    21061 cagcatccac gctaagctgt ggctgctcga taattttggc tgcgcccgct ttttccagcg
    21121 gtagcgcatt ccagtattgc tggcggtctt tatgttgaaa cggcacaaac aacgccggta
    21181 gtcctgccgc ggcgatttca ctcaccgtta aagcaccgga gcggcaaacg acgacatccg
    21241 cccacgcata cgccgccgcc atatcatcaa taaattccgt cactttatgc tgcggttgcc
    21301 ccgcttcggc atacgcctgt tcaacggatt gttgcgaacc tttaccgctc tgatgccaga
    21361 tagtgactga atcacccagt ttcgcagcaa cctgcggcat tgtctggtta agaatgcgtg
    21421 cgccctggga accacccact accagcacac gaaccggacc ttcacgtcca gccaaacgtt
    21481 gctgcggcaa cggcagcgcc agcacatcgg tacgcaccgg gttacccacc acttccgcat
    21541 tagggaaagc gcctggaaac gcctgcatca ctttggtggc aatcttcgcc agccatttat
    21601 tggttaagcc cgcaataccg ttttgttcat gaagtacaac cggaatgcct aacgaccacg
    21661 cggccagacc acctggacct gatacatagc cgcccatacc gagtaccacg tcaggtttgt
    21721 acgctttcat aatcgcccgc gcctgacgcc aggcgttgaa gatacgcagc ggcgcagcta
    21781 tcagtgcttt tatacctttt ccacgcagac cagagatacg aatgaaatca atttcgatgc
    21841 catgttttgg cactaagtcc gcttccatac ggtcggcagt ccccagccag cgaacttgcc
    21901 aaccctgagc cattagatgg tgcgcaaccg ccagtcccgg gaatacatgt ccaccggttc
    21961 cgcctgccat caccattaat cgctttcctt gaccactcat cgtgaacctc gtacaaacgc
    22021 ctgcgctttc tccagacgcg tttcataatc aatacgcaac agcatcatga tggctgtcga
    22081 cataatcagt aagctcgaac caccgtaact gatcagcggc aatgtcagac ctttggtcgg
    22141 taacatcccc gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc agatgccaat
    22201 agaacaggcg agaaaaccgg aaaaacggtg gtcaatttct aacgctttgc ggccaatcga
    22261 catcgcgcga aaagcgacga agaataccat taaaagtgcc agcaccacac cgacataccc
    22321 cagttcttcg ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca gatactccag
    22381 tttttgtacc gagttaccta aaccttgtcc ccaaagttcg ccgcgaccaa acgccatcag
    22441 cgattgcgtt aactgatagc cgctgccaaa gggatcttcc cacgggttcc agaatgcggt
    22501 aacacggcgg atacggtacg gttcggcgag tatcagcaac acaaccgctg aaatgcccat
    22561 accgataatg gcaatgaact gccacaattt cgctcccgcc aggaacaaca tcgccagcgt
    22621 agtcacaaac aacaccacca ccgtaccaag gtctggctgt gccagcagta acactgccaa
    22681 caccagaatc acgcccatcg gtttcaggaa gccgcgcagg ttattacgta cttcgtcgcc
    22741 tttacgcacc agatagttgg cgatataaca aaacagcgac agttttgtca gctccgcagg
    22801 ctggatacgc agcaaaccga gatcgatcca acgcgatgcc cctttaaccg agctacccac
    22861 taccagcacg atcatcagca ggatgataga tccgagcagc atcgtggcac tgtagcgttg
    22921 ccagaactcc atcggcagac gcagcgtaat gatcgccaga atgaatgcca ggatcagata
    22981 gacgccatca cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc ctatgggcat
    23041 tgacgccgag gtcaccatga taaagccaat cgccgcaagg ccgaaggtca gccacagtaa
    23101 ggtgcgatcg tacatgatca ggctgtcggt atctttttcc cgcgagccca tcacccagcc
    23161 ttttagcgcc gtggagatcc agaccaggat actgaatcct ggcaggcgcg gcattttcag
    23221 gcgagggaga gataaacgca tcaacctaac tccttcgcca gacgggcaaa ctcattgcct
    23281 cgttgttcaa agttcttgaa ctgatcaagg ctggcacagg ccggggagag cagaaccata
    23341 tcgcccagct gaacacgcgg agccagcaag cgcatcgctt gttccatggt ttcggtttgt
    23401 tctgccactt ccgggcgtag cgccgccagc tgcgcaccgt cacgaccaaa acagtacaaa
    23461 cgcacgttgt cgccattcag atagcgcgcc agtggactga agtccgccga tttaccatcg
    23521 ccgcccagca acaaatgcag cgtgccgtct acctgcaagc cattcaacgc cgcttccgta
    23581 ctgccgacgt tagtggcttt cgaatcgtta atccagcgta cgccattgtg ctccagcacc
    23641 acttcaaagc gatgcggcag accggtgaag gtggttaacg ctttcaggct gctggcgcgc
    23701 ggcaaaccag ctgcgtctgc cagtgccagt gctgccagcg cattggtgta gttatgctgc
    23761 ccggaaagtt tcatctcttt cacattcagc actttctcgc ctttaacccg cagccaggtt
    23821 tcgccctgct gatgattcag gtgatagtca cccatgttga cgccaaagct gacgcagcgt
    23881 tcatcagcac cgcgaatcgg cattgttaag gcatcatcag cattgacgac acaaactttc
    23941 gcattttcgt aaatacgcaa ttttgccgca cggtattgct gcaaaccaaa cggatagcgg
    24001 tccatatgat cttcagtcac gttcagaatg gtcgccgcca ccgcctgtaa gctggaagtg
    24061 gtttccagct ggaagctcga cagttccagc acgtacagtt cgcactcagc atcaagcagc
    24121 atcaacgctg gcaggccaat attgccaccc acaccaacgt taacccccgc cgctttcgcc
    24181 atttcaccca ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat cgccacaatc
    24241 ggtgcttgtg cttcgcgaca gaacagctcg atatcgccaa cgatttcgat tccggcatca
    24301 gcggcagcgc ttaaggatgg atgcgcctgt gcaataccgg gactggcgac aatcagatct
    24361 gccgccatca gccattcatc attcagactg cccgtgtggc gttctacggc ttcgggtaat
    24421 ttatccaggc caggcggtgt catacgcgta tccataacgc gcggcgtcac accgcgagcg
    24481 aggaaaaagt ccacgcagga aagcccggtg aggcccaggc cgataatgac gacattttta
    24541 ccctgataat cagccatgat taacgtacct tcagcgttgc cagaccaatc agaaccagca
    24601 tcagcgaaat aatccagaaa cgcacaatga cgcgcggttc cggccagcct ttcagttcat
    24661 agtggtgatg aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta aaggagccga
    24721 cctgcaggat gacagaaagc gtttctacca cgaacacgcc ccccataatc accagcagga
    24781 attcctgacg tagcagtacg gcgataatgc ctaacgcacc acctaacgcc agcgaaccta
    24841 catcgcccat aaagacctgc gccggatagg tgttaaacca caggaagccc agtcctgccc
    24901 cgactatcgc cgtgcagaca atgaccagtt cccctgcgtg tcgcagatac ggtatatgca
    24961 agtagctggc aaagttcata ttgccggtcg cccacgccac cagcgcaaaa ccaccggcga
    25021 caaatacggt cggcataatt gccaggccgt cgagaccatc ggtcaggttt accgcgttgc
    25081 cagtacccac aatgacgaag taagccagca gaatgtagaa cagccccagc tgcggcatca
    25141 catctttaaa gaatgggacc accagctgcg ttgcgggcgt gtctttgccg gcaaggtaca
    25201 gggcgaaggc gacacccagc gcaatgaccg acatccagaa atacttccaa cgagcgatca
    25261 accctttggt gtctttacgc accactttgc gataatcatc aacaaagcca ataacaccgt
    25321 aacctaccag caccaccaac acgcaccaga cgtacggatt ggacgggtaa gcccacagca
    25381 gtacggagat cacaatcgcc gtcaggatca taatcccgcc catggtcggc gtaccgcgct
    25441 tgctgaagtg tgattcagga ccgtcgttac gcaccacctg accaaaggaa agtttttgca
    25501 aatgagctat catacgcggg cccatccaca atgagatgaa cagcgcggtc agcaggctga
    25561 cgatggcgcg aaacgtcaga taggaaaaga cgttaaagcc ggaataatat ttgaccaaat
    25621 gttcggccag ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta cctcttccat
    25681 ggcggcacta cgtgaaccct taactaaaat cgtaattacc tgttgctcag caatcagtga
    25741 tttaagacgc gtaattaacg cagttttatc agcaaaatgt tcgccaacgc cgctggcggt
    25801 gctgatagca tggctttgtt tacccacgct taacacgcgg tcaataccag cagcttttgc
    25861 cgcctcgccc acctgtacat ggcaggcttc gctttcagcg cccagttccg ccatatcgcc
    25921 caccaccagc acgcggtagc ccggcatttc agccagtacc tgaacagcgg cggtcattga
    25981 accgacattg gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca gttggatggg
    26041 gaacagacgg cctggaacag ctttcagatt tgccagcccc gctttgatag cattaagcgt
    26101 tgcgcccacg gacatggaga gcgcagcggc tgccagcgca ttcgcaatat tgtgacgccc
    26161 cggcaacggc agcagaacat cgacgctacc ggttggggtt tgtagggtaa attccgtacc
    26221 gtgcgaggtc acatggatat tggtggcggt gaaatcgctg ttggcggcat tgggtgagaa
    26281 acgccacact ttgcgtgagc caattacgct ctgccagttc agccagtcgt tgttgtcggc
    26341 gttcataatg gcgataccgt tttccggcag gccgctaaag atttcacctt tcgctttcgc
    26401 gacacccgca agcgagccaa aaccttccag atgcgccgct gccaggttgt tgaccagcgc
    26461 agcttccggg cgagtcagac tcacagtcca ggctatttcg ccctgatggt tcgcgccaag
    26521 ttcaataact gcgtaatcgt attccggcgt taagcgcaac aacgtcatcg gtacaccgat
    26581 gtcgttgttg agattgcctg ccgtataaag cgtgttgccg cactggctta aaatcgccgc
    26641 cgtcatctct ttaacggagg ttttgccgga ggaacctgtc agagcaacca cgcgcgccgg
    26701 aacttgctgg cgaacccatg cagccagttc accaaacgcc agacgcgtat ccttgacgat
    26761 taactgcggc aggtcgatat ccagcggacg gctgaccagt agtgcgcccg cgccgccagc
    26821 tttcgcctgg tcggcaaaat catgagcatc gaaacgttcg cctttcaggg caacaaacag
    26881 gcagcccggc gtcagttttc gggtatcagt ggttacagca tcaagggtga tatctgcacc
    26941 ttgcagttca ccgttgagaa tgtcggtaag ttggctaagg gttacgctaa tcatgcaatc
    27001 acccccagca gacgcgccac cgtgacgcga tcggagtagt ccagacgctg attgccaaca
    27061 atctggtaat cttcatggcc tttgcccgcg accagtacca catcattctc tttagcctgc
    27121 ataacggcgc aagtcaccgc ttcagcacgg ccttccatca ctttggcatg tccggcatct
    27181 aacattcccg ccagaatatc gttgatgatg gcacgcggtt cttcggtacg cgggttatcg
    27241 tccgtcacca ccgccacgtc agcaaactct tcggcaattg cgcccatcag tggacgctta
    27301 cctttatcgc gatcgccacc acagccaaag acgcaccaca gcttgcccgc acagtgcagg
    27361 cgcgccgcct gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc caccaccacc
    27421 gtcggtttgc ctggcgcagt gaacacttcc atacgtccgc aaaccggttg cagacgcgcg
    27481 gcggttttca gcagatcagc cagtggatag ccgagtgcca gcagtgtcgc cagcgcgagc
    27541 agcaggttgc tgacgttaaa agcgcccatc agatggcttt caatttcgcc atcgccccaa
    27601 cttgagctaa agcgaatcgt cgcaccgctg tcgtgatagt tcacgtcgat cgcttttaac
    27661 cagcgtccgt gacagttcgg attaatatga tcttccattg ataccgcaac cgcgtccggc
    27721 agttttgcca gccagcggcg gcccacttca tcgtcggcgt taataatcgc ctgaccgcaa
    27781 tgatgttcag aataaagcag ccatttcgca gcttcgtagt gttccatatc accatgataa
    27841 tcaaggtgat cgcggcttaa gttggtaaag accgacgccg caaatttcaa tgccgccaca
    27901 cggtgctgta ccagcccgtg ggaggaaact tccattgcgc aaaacgtcgc gccctgctcc
    27961 accagccccg ccagctcatg ctgaacatcg actgcggaac cggtagtatt ttctgtcggg
    28021 atcactttcc caagcagacc gttaccaacg gtgcccatta ccgcgctggt ttcgccaagc
    28081 agttggctcc actgcgccag cagctgggta gtcgtggttt tgccgttggt gcccgttacg
    28141 cctacgagac gtaaattgtc agagggttca tggtaaaagc ggcccgccag tgcagataaa
    28201 cgctcattga gctggctgag atagatgacc ggtacgccgt gcatttcacg gatttcaccg
    28261 tcggtcgcct catctttcgc tttcgcctct gcaataatgg cagccacacc ttgcgctatc
    28321 gcctgcggga tatatcgacg cccgtccgcc tgatgaccta ctacagctac aaagagatcg
    28381 cccgccgcag ccacacggct gtcgagtgtc atctctcgca gtgctcgcga aggtgcgtct
    28441 ggcacccacg gagcaagaag gtcgcgcaaa ttacgatctg ccacctgtcc cctcgccttg
    28501 attaatcaca aattcatttt tatcgcccgt tgtcagcgca tccggctcga tgttcatggt
    28561 acgcaatacg ccgcccatga tggcaccaaa gaccggcgcg gaaacggcgc cgccgtagta
    28621 tttacccgcc tgcggatcgt tgataacaac aaccagcgcg aagcgcggct gactcgcagg
    28681 cgcaacgcct gcggtataag caatatattt attgatgtag cgaccgtccg gcccgacctt
    28741 tttcgcggta ccggttttaa tggcgatacg atagccttta atcgccgcct tcacgccgcc
    28801 gccgcctggt agcgccacgc tttccatcat atgcaccaca gtgcggacaa tggatcccgg
    28861 gaagacacgt tcaccgggaa ccggggggtc aactttggta atcgacagtg ggcgataaat
    28921 gccgtagctg ccgatagttg cgtagactcg cgctaactgt aacggtgtta ccattagccc
    28981 gtagccgaaa gagaaggtgg ccctctctat gtcagaccac cgttgttttt gaggatataa
    29041 gccactgcgt tctccgacca accccaaatt ggtcgctttt cccagtccaa aacgtgagta
    29101 agtatctact aacgctgagg acggcatcgc taacgccagc ttggaaacac cgacgttact
    29161 cgacttctgt aataccccgg tcagggttaa ttcgctgtag cgtgccacgt ctttgatttc
    29221 gtggccgtta attcgataag gaatggtatt gagtaccgag ttttcccgca ccacgccacg
    29281 ttgcaacgcg gtcattacca ccatcggttt aaccgttgag cccggttcaa acacgtcggt
    29341 gatggtacgg ttacgcatcg cctctttcgg cgtgccgctc agattgttag ggttgtatga
    29401 cgggctgtta gccatcgcca gcacttcacc ggtgttgaca tccaccagca cggcgctacc
    29461 agattcagcc ttgttaaagg ccaccgcgtt gttcagttcg cgataaacca gcgcctgcag
    29521 gcgttcatca atactcagcg ccaggttgtg cgctgcctgg ctgtcagtag aagaaatatc
    29581 ttcaattacg cgaccatagc ggtctttacg cacaatgcgc tcacccggct gcccggtaag
    29641 ccatttatcg aaactcttct caacgccctc aatcccttga ctatcgacgt tagtaaagcc
    29701 gatgaggtga gcagtcactt cgccggacgg atagtaacgg cgagactctt cacgcagatg
    29761 aatccccggc agtttcagtt ttttgatgta gtccgccatg tcagggttca cctgacgcgc
    29821 cagataaata aagcgccctt tcgggttggc gttaatgcgg gctgaaagct gatccagcgg
    29881 aatattgagc gcgttagcca gcgccttcca gcggtcaccg acgctgatac cgccagcgtc
    29941 atgcacttct ttcgggtcag cccaaatcgc ttttaccggc acgctcaccg ctaacgggcg
    30001 accagaacgg tcagtaatca tgccgcggga ggtggaaact tgctgaacgc gaagagaacg
    30061 catgtcgccc tctttcacca gcatatccgg ggagataact tgtaaccacg ctacgcgtcc
    30121 gagcagaaaa gccagcgcca ggagaataca gccgcataac aacgcaaaac gccaactgat
    30181 aaagttggca tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg ctttcatgcg
    30241 tcgcgtttat ccttattttt gcactacgat attttcttgt gacggatcaa catgctgcat
    30301 ttgcagcttt tccgtggcga tcctttccac ccggctatgg tcgccgagcg cattctcttc
    30361 aaggatcagg ttgcgccatt caatgtctaa agcatctcgc tccagcacca gttgttcgcg
    30421 ctgagcggtc agtaaacggg tatggtgcgc cgtggttacc acagtcaccg ccgtcaaaat
    30481 aatgcaaatg aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt caccgataac
    30541 accaggcaat gcatggcgct cgtggcttcc catcgatcct ttaactttgc ttagagcttc
    30601 tgtcactctg ctgatcatgc attcgtcctc tctgcaatac gcagaactga actacgggca
    30661 cgagggttct cagccacctc ttcttcgccc ggcattaact tgcctagtgc tcgcagctga
    30721 cggccaccca gttttttgag ctgctcttca gtcatcggta accctgccgg aacttgcgga
    30781 ccgcggctgt tttcacgcat aaaacgtttc acaatacggt cttccagcga gtggaagctg
    30841 atgatcgaaa gccgcccacc cggggccagc acgttgagcg agctttttag cgcctgctct
    30901 atctcctcca gttcactgtt tacccaaatg cgcaccgcct ggaaggtacg ggtcgcggga
    30961 tgtttaaact tatctttcac cggcgttgca acagccacga cttccgccag ttctttggtg
    31021 cgggtcatcg gctgttcgcg gttacgctcg acaatggcgc gggcaatgcg tttggcaaaa
    31081 cgctcttcac cataggtttt caatacccaa gcgatatcgg cttcttctgc ggtttgtagc
    31141 cattcagcgg ctgactgccc acgggttggg tccatacgca tgtccagcgg accatcgcgc
    31201 ataaaggaaa agccgcgctc agcatcatca agttgcggtg aagagacgcc aagatcgagg
    31261 agaatgccgt cgatcttgcc gataagatcg cgctcggcaa cgtattcgcc cagcgcggag
    31321 aaaggtccgt ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac ggcgatagcc
    31381 tgcgggtcgc gatcgatcgc cagcaaacgc ccctcttcgc caagctgcga gaggatcaga
    31441 cgtgagtgac caccgcgacc aaaagtccca tcaatgtaga tgccatcagg acggatattg
    31501 aggccattaa cggcttcatc cagcagcacc gtagtatgtt tatagttttc catcatttta
    31561 tagagacaag tcctgcagtc gctccgataa gtctccggta gccaactgct ctgcgtcgat
    31621 atcttccttg acctgttgat gccaggttgt ttcatcccac agctcaaact tgttgaactg
    31681 tccaaccagc atcacttctt ttgtcagccc ggcatgttgc cgcagtactg gcgcgattaa
    31741 caatcgacct gcgccatcca tctgacattc gctggcatga cctaacagta ggcgctgcac
    31801 acggcgctca accgggttca tgctcgacag acgcgataat ttttgctcga taatttccca
    31861 ttcaggcagg gggtaaagca gcaggcacgg gtgatgaatg tcaatggtgc aaaccatttg
    31921 accgacagcg ttctcaagca gctgttcccg gtaacgggta ggcactgata agcgcccttt
    31981 gctgtcgaga ttgactaacg ttgctccccg gaacatgcca gcctcacccc ttattcccac
    32041 tttgccccac aatttcccac tgaaaggagt ttacggagcg gaggaaaagc ttgtcaagcc
    32101 agtcacaagg cataccgagg taaaaaaccc gatgtttacg gctaatatca gcgttgttta
    32161 ttttacgtcc aacgtgcgca gcaaattaac gttatgaata gctgtaagaa aaaatccctc
    32221 atcgtcatca ccgttttaaa atgactcact attttccaca gcaaatataa agtgtcagtt
    32281 tgcgacgcga gcggcatttt aggacatatc ttccccggtt aacagtccct gttgcgtctg
    32341 tgtggcgctt gatgccagcg ggtttgcgca aaagcaaaga aaagtgcttg ttaaatcggc
    32401 aattcatctc tgcatgtaac aaaataatgc aaaaacgcgc tgtcgatatc acttaattat
    32461 tgtgaatttc agctggtgag ttaattaacg ggcgggttta aaagaactta tcaataatag
    32521 tcaggaatag tcttatttac tttaaggata ttgatgtcca ttcccgtact ctacg
//
LOCUS       DAZRUF010000017        31629 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZRUF010000017 DAZRUF010000000
VERSION     DAZRUF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.017"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            158..493
                     /gene="eutS"
                     /locus_tag="ACHXOL_000675"
     CDS             158..493
                     /gene="eutS"
                     /locus_tag="ACHXOL_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR6807734.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            506..985
                     /gene="eutP"
                     /locus_tag="ACHXOL_000676"
     CDS             506..985
                     /gene="eutP"
                     /locus_tag="ACHXOL_000676"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR6807735.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            960..1661
                     /gene="eutQ"
                     /locus_tag="ACHXOL_000677"
     CDS             960..1661
                     /gene="eutQ"
                     /locus_tag="ACHXOL_000677"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR6807736.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1658..2461
                     /gene="eutT"
                     /locus_tag="ACHXOL_000678"
     CDS             1658..2461
                     /gene="eutT"
                     /locus_tag="ACHXOL_000678"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR6807737.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            2458..3474
                     /gene="pta"
                     /locus_tag="ACHXOL_000679"
     CDS             2458..3474
                     /gene="pta"
                     /locus_tag="ACHXOL_000679"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR6807738.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3513..3806
                     /gene="eutM"
                     /locus_tag="ACHXOL_000680"
     CDS             3513..3806
                     /gene="eutM"
                     /locus_tag="ACHXOL_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR6807739.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3913..4200
                     /gene="eutN"
                     /locus_tag="ACHXOL_000681"
     CDS             3913..4200
                     /gene="eutN"
                     /locus_tag="ACHXOL_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR6807740.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4212..5615
                     /locus_tag="ACHXOL_000682"
     CDS             4212..5615
                     /locus_tag="ACHXOL_000682"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR6807741.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            5626..6462
                     /gene="eutJ"
                     /locus_tag="ACHXOL_000683"
     CDS             5626..6462
                     /gene="eutJ"
                     /locus_tag="ACHXOL_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR6807742.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6452..7639
                     /gene="eutG"
                     /locus_tag="ACHXOL_000684"
     CDS             6452..7639
                     /gene="eutG"
                     /locus_tag="ACHXOL_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR6807743.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7956..9182
                     /gene="eutH"
                     /locus_tag="ACHXOL_000685"
     CDS             7956..9182
                     /gene="eutH"
                     /locus_tag="ACHXOL_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR6807744.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            9179..10582
                     /gene="eutA"
                     /locus_tag="ACHXOL_000686"
     CDS             9179..10582
                     /gene="eutA"
                     /locus_tag="ACHXOL_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR6807745.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10594..11955
                     /gene="eutB"
                     /locus_tag="ACHXOL_000687"
     CDS             10594..11955
                     /gene="eutB"
                     /locus_tag="ACHXOL_000687"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFR6807746.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNRSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11976..12863
                     /gene="eutC"
                     /locus_tag="ACHXOL_000688"
     CDS             11976..12863
                     /gene="eutC"
                     /locus_tag="ACHXOL_000688"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR6807747.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12873..13532
                     /gene="eutL"
                     /locus_tag="ACHXOL_000689"
     CDS             12873..13532
                     /gene="eutL"
                     /locus_tag="ACHXOL_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR6807748.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13545..14045
                     /gene="eutK"
                     /locus_tag="ACHXOL_000690"
     CDS             13545..14045
                     /gene="eutK"
                     /locus_tag="ACHXOL_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR6807749.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            14091..15143
                     /gene="eutR"
                     /locus_tag="ACHXOL_000691"
     CDS             14091..15143
                     /gene="eutR"
                     /locus_tag="ACHXOL_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR6807750.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(15149..16048)
                     /gene="hemF"
                     /locus_tag="ACHXOL_000692"
     CDS             complement(15149..16048)
                     /gene="hemF"
                     /locus_tag="ACHXOL_000692"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR6807751.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16052..16921)
                     /gene="amiA"
                     /locus_tag="ACHXOL_000693"
     CDS             complement(16052..16921)
                     /gene="amiA"
                     /locus_tag="ACHXOL_000693"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR6807752.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLPGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            17135..17560
                     /locus_tag="ACHXOL_000694"
     CDS             17135..17560
                     /locus_tag="ACHXOL_000694"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR6807753.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17547..17996
                     /locus_tag="ACHXOL_000695"
     CDS             17547..17996
                     /locus_tag="ACHXOL_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR6807754.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            18057..18632
                     /locus_tag="ACHXOL_000696"
     CDS             18057..18632
                     /locus_tag="ACHXOL_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR6807755.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18728..19627
                     /gene="yfeX"
                     /locus_tag="ACHXOL_000697"
     CDS             18728..19627
                     /gene="yfeX"
                     /locus_tag="ACHXOL_000697"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR6807756.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19685..20989)
                     /gene="pbp4b"
                     /locus_tag="ACHXOL_000698"
     CDS             complement(19685..20989)
                     /gene="pbp4b"
                     /locus_tag="ACHXOL_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR6807757.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20994..22418)
                     /gene="murP"
                     /locus_tag="ACHXOL_000699"
                     /pseudo
     CDS             complement(20994..22418)
                     /gene="murP"
                     /locus_tag="ACHXOL_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(22422..23318)
                     /gene="murQ"
                     /locus_tag="ACHXOL_000700"
     CDS             complement(22422..23318)
                     /gene="murQ"
                     /locus_tag="ACHXOL_000700"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR6807758.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23482..24339
                     /gene="murR"
                     /locus_tag="ACHXOL_000701"
     CDS             23482..24339
                     /gene="murR"
                     /locus_tag="ACHXOL_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR6807759.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24468..25259
                     /gene="ucpA"
                     /locus_tag="ACHXOL_000702"
     CDS             24468..25259
                     /gene="ucpA"
                     /locus_tag="ACHXOL_000702"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR6807760.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25430..26446
                     /gene="cysP"
                     /locus_tag="ACHXOL_000703"
     CDS             25430..26446
                     /gene="cysP"
                     /locus_tag="ACHXOL_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR6807761.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26446..27279
                     /gene="cysT"
                     /locus_tag="ACHXOL_000704"
     CDS             26446..27279
                     /gene="cysT"
                     /locus_tag="ACHXOL_000704"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR6807762.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            27279..28154
                     /gene="cysW"
                     /locus_tag="ACHXOL_000705"
     CDS             27279..28154
                     /gene="cysW"
                     /locus_tag="ACHXOL_000705"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR6807763.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            28144..29241
                     /gene="cysA"
                     /locus_tag="ACHXOL_000706"
     CDS             28144..29241
                     /gene="cysA"
                     /locus_tag="ACHXOL_000706"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR6807764.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29375..30286
                     /gene="cysM"
                     /locus_tag="ACHXOL_000707"
     CDS             29375..30286
                     /gene="cysM"
                     /locus_tag="ACHXOL_000707"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR6807765.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30289..30657)
                     /locus_tag="ACHXOL_000708"
     CDS             complement(30289..30657)
                     /locus_tag="ACHXOL_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR6807766.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30762..31613
                     /gene="pdxK"
                     /locus_tag="ACHXOL_000709"
                     /gene_synonym="thiJ"
     CDS             30762..31613
                     /gene="pdxK"
                     /locus_tag="ACHXOL_000709"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR6807767.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         7160 a         8176 c         9153 g         7140 t
ORIGIN      
        1 atattcatgc gttgcatatg aaagtttatg caccacagcg aatatctctc cattcttagt
       61 gatctacctc accttttaaa cgcgcttgcc gaattttgtt atttactctg acgaaaaatt
      121 gtcacgatac acgaaagttt ttcacaggcg gcgactcatg gataaagaac gcatcattca
      181 ggaatttgtg cccggcaaac aggtcacact ggcgcatctc atcgcgcatc ccggtgagga
      241 actggcgaag aaaatcggcg ttccggatgc gggcgcaatt ggcattatga cgctaactcc
      301 cggcgaaacg gcgatgatcg tcggtgattt agcgctgaaa gccgctgatg tgcatatcgg
      361 ttttctcgac cgcttcagcg gtgcattggt gatttatggc tcggtgggcg cagtagaaga
      421 agcgttatca caaaccgtca gcggtttagg tcgcttatta aattatacgc tttgtgaaat
      481 gaccaaaagt taatacgagg cattcatgaa acgaattgcg tttgtgggta gcgtcggggc
      541 aggaaaaaca acgttattta atgcgttgca ggggaattat accctcgcca gaaaaacaca
      601 ggccgtggaa tttaatgata agggcgatat tgatacgccg ggtgaatatt ttagccatcc
      661 ccgctggtat cacgccttaa ttaccacgct acaggatgta gacatgctga tctatgtcca
      721 tggcgcaaat gacccggaaa gtcggttgcc tgccgggttg ctggatattg gcgtcagcaa
      781 acggcaaatc gccgtcatca gtaaaacgga catgccagat gctgacgtcg ccgcaacgcg
      841 aaagttattg cttgagacgg gtttcgaaga gccgattttc gagctaaaca gccacgaccc
      901 gcaaagcgtg cagcaactgg tggattatct ggcatctctc accaaacagg aggaagcagg
      961 tgaaaaaact catcacagcg aatgatattc gtgaagcaca cgcgcgcggc gaacaggcaa
     1021 tgtccgtggt tctgcgcgcc agcattatta ccccggaagc ccgtaaagtg gcggatctac
     1081 tgggttttac catcaccgaa tgtgatgaat cgatcccggt aacggcgtcc gtgcccgcca
     1141 gcgtgccagc cgacaaaacc gaaagccagc gcatccgcga aaccatcatt gcccagctgc
     1201 cggaaggcca gtttaccgaa agcctggtcg cacagctgat ggaaaaagtg atgaaggaaa
     1261 aacagtcgct ggagcagggc gcaatgcagc cgagcttcaa atcggtgacc ggcaaaggcg
     1321 gcatcaaagt gattgatggc agcagcgtca agtttggtcg ttttgatggc gcacaaccgc
     1381 attgcgtggg cttgactgat cttgtgaccg gagacgacgg cagcagcatg gccgccggtt
     1441 ttatgcagtg ggagaacgca ttcttcccgt ggaccctgaa ctacgacgaa atcgacatgg
     1501 tgctggaagg ggagctccat gttcgccacg aaggcgaaac catgatcgcc aaagcaggcg
     1561 acgtgatgtt tatcccgaaa ggctccagca tcgaatttgg tacgacatcc agcgtgaaat
     1621 tcctgtatgt cgcctggccg gctaactggc aatccctatg aaagatttca tcaccgaagc
     1681 atggctaaga gcgaaccata cgctcagcga aggcgcagag atccatctcc ctgcggacag
     1741 tcgcctgacg ccctctgccc gggagttgct ggaaagccgc catctgcgca tcaagtttat
     1801 tgacgagcag ggccgcctgt ttgttgacga tgaacagcag cagccgcagc ccgttcatgg
     1861 gctgaccagt agcgatgaac atccgcaggc gtgctgcgaa ctgtgccgcc agccggtggc
     1921 aaaaaagccg gatacgctga ctcacctgtc ggcggaaaaa atggtcgcca aaagcgatcc
     1981 gcgtctgggt tttcgcgcgg ttctcgacag caccattgcg ctggcagtgt ggctgcaaat
     2041 tgaactggcg gaaccgtggc agccgtggct ggcggatatc cgttcgcgtc ttggcaacat
     2101 tatgcgcgcc gatgcgctgg gggaaccgct gggctgccag gcgattgtcg ggctttctga
     2161 cgaagatctg catcgacttt ctcaccagcc gctgcgctat ctcgaccacg atcatctggt
     2221 gccggaagcc agccacgggc gcgatgcggc gttgctcaat ctgctacgta ccaaagtgcg
     2281 ggaaaccgaa accgtagcgg cgcaggtgtt tatcacccgc agctttgaag tattacgccc
     2341 ggacatcttg caggcgctga accgcctttc gagcacggtc tacgtaatga tgatcctgag
     2401 cgtaacgaag ccgccgctaa cggtgaagca gatccaacaa cgactggggg aaacgcaatg
     2461 attattgaac gttgtcgtga actggcgttg cgagcgcccg ccagagtggt ttttccggat
     2521 gcgttagatc aacgtgtgct gaaagctgcg caatatttac atcaacaagg tctggcaacg
     2581 cccattctgg tcgctaatcc gtttgaactt cgtcagtttg cgctcagtca cggcgtggcg
     2641 atggacgggc tacaggtgat tgatccgcat ggcaacctcg caatgcggga agaatttgct
     2701 catcgctggc tggcccgcgc gggcgaaaaa acgccgccgg atgcgctgga aaaacttacc
     2761 gatccgctga tgttcgccgc agcaatggtc agcgccggta aagcggatgt ctgtatcgcg
     2821 ggcaacctct cttccacggc gaatgtgctg cgtgccggat tacgtattat cggcttgcag
     2881 ccaggctgta aaacgctctc atccattttc ctgatgctgc cacagtacag cggtccggcg
     2941 ttgggctttg ccgattgcag cgtggtgcca cagccgacgg cggcgcaact ggcggatatc
     3001 gcgcttgcca gtgccgaaac ctggcgcgcc ctcaccggag aagagccgcg cgtggcgatg
     3061 ctgtcgtttt ccagtaacgg tagcgcccgt cacccctgcg ttgccaacgt ccagcaggcg
     3121 acagaaatcg tccgtgagcg cgcaccaaag ctggtggttg atggcgagtt gcagtttgac
     3181 gccgccttcg tgccggaagt ggcggcgcaa aaagcgcctg ccagcccgct acagggcaaa
     3241 gccaatgtga tggtttttcc gtcgctggaa gccggaaata ttggctacaa aatcgcacaa
     3301 cgactcggcg gatatcgtgc cgtcgggcca ttgatacaag gacttgccgc gccgatgcac
     3361 gatctctctc gtggttgtag cgtgcaggaa attatcgagc tggcgctggt ggcagctgtg
     3421 ccgcgtcaga cagaagtgaa ccgcgaaagc agtttacaaa cactggttga atgaacggtc
     3481 ccgttctgga ccctgataaa gaggaaagca caatggaagc attaggaatg atcgaaaccc
     3541 ggggcctggt tgcactgatt gaggcttctg acgcgatggt taaagctgcg cgcgtcaagc
     3601 tggttggtgt taagcagatt ggcggcggcc tgtgtactgc catggttcgt ggtgatgttg
     3661 cagcctgtaa agccgcgacc gatgcaggtg ccgcagcggc acagcgtatc ggcgagctgg
     3721 tatctgtaca cgttatcccg cgtccgcacg gcgacctgga agaagtcttc ccgatcggcc
     3781 tgaaaggcga tagcagcaac ctgtaatcac cctgtgtgac gtcgattcgg gcgtcacgct
     3841 ctctcctttt tcattttacc ttctgcggag ggtaggggga gcttttacct gaaatacgcc
     3901 acggaggcgg gtatgaaact ggcagtcgtc actggacaaa ttgtttgtac cgtacgccat
     3961 cacggactgg cgcatgacaa attgctgatg gtggaaatga ttgatccaca aggtaatcct
     4021 gacgggcaat gcgccgtcgc catcgacaat attggcgcgg gaaccgggga gtgggtgtta
     4081 ctggtgagtg gcagttccgc ccgccaggcg cataaaagcg aaacgtcacc ggtcgatctg
     4141 tgcgtgattg gcattgtcga tgaggtggtg tctggcggtc aggtaatttt ccacaaataa
     4201 ggcagaacat catgaatcaa caggatattg aacaggtggt gaaagcggta ctgctgaaaa
     4261 tgcaaagcag tgacacgccg cccgccgccg ttcatgagat gggcgttttc gcgtccctgg
     4321 atgacgccgt tgcggcagcc aaagtcgccc agcaagggtt aaaaagcgtg gcaatgcgcc
     4381 agttagccat tgctgccatt cgtgaggcag gcgaaaaaca cgccagagat ttagcggaac
     4441 ttgccgtcag tgaaaccggc atgggccgcg ttgaagataa atttgccaaa aacgtcgctc
     4501 aggcgcgcgg cacgccaggc gttgagtgtc tctctccgca ggtgctgacc ggcgacaacg
     4561 gcctgacttt aattgaaaac gcgccctggg gcgtggtggc ttcggtaact ccgtccacta
     4621 acccggcggc aaccgtaatt aataacgcca tcagcctgat tgccgcgggc aacagcgtca
     4681 tttttgcccc gcatccggcg gcgaaaaaag tctcccagcg ggcgatcacg ctgctcaacc
     4741 aggcgattgt tgccgcaggc gggccggaaa acttactggt tactgtggca aatccggata
     4801 tcgaaaccgc gcagcgtttg ttcaagtttc cgggcatcgg ccttctggtg gtgaccggcg
     4861 gcgaagcggt agtagaagcg gcgcgtaaac acaccaataa acgtctgatt gccgcaggcg
     4921 ctggcaaccc gccggtagtg gtggatgaaa ccgccgacct cgcccgtgcc gctcagtcca
     4981 tcgtcaaagg cgcttctttc gataacaaca tcatttgtgc cgacgaaaag gtactgattg
     5041 ttgttgatag cgtagccgat gaactgatgc gtctgatgga aggccagcac gcggtgaaac
     5101 tgaccgcaga acaggcgcag cagctgcaac cggtgttgct gaaaaatatc gacgagcgcg
     5161 gaaaaggcac cgtcagccgt gactgggttg gtcgcgatgc ggcgaaaatc gcggcggcaa
     5221 tcggcctgaa cgtgccgcaa gaaacgcgtt tgctgtttgt ggaaaccact gcagaacatc
     5281 cgtttgccgt gaccgaactg atgatgccgg tgctgcccgt cgtgcgcgtc gccaacgtgg
     5341 cggatgccat tgcgctggcg gtgaaactgg aaggcggttg ccaccacacg gcggcaatgc
     5401 actcgcgcaa catcgaaaac atgaaccaga tggcgaatgc tattgatacc agcattttcg
     5461 ttaagaacgg accgcgcatt gccgggctgg ggctgggcgg ggaaggctgg accaccatga
     5521 ccatcaccac gccaaccggt gaaggggtaa ctagcgtgcg tacgtttgtc cgtctgcgtc
     5581 gctgtgtatt agtcgatgcg tttcgcattg tttaaggagt aacagatggc gcacgacgaa
     5641 caatggctca ccccacgtct gcaaacggcg gcaacgctgt gtaaccagac gcccgccgcg
     5701 acggaatctc cgctgtggct gggggtggat ctgggcacct gcgatgtggt gtcgatggtt
     5761 gtcgaccgcg acggtcagtc ggtggcggta tgcctcgact gggccgatgt ggtccgcgac
     5821 ggcatcgtct gggatttctt cggcgctgtc accattgttc gtcgccatct cgacacgctc
     5881 gagcagcaat tcggtcgccg ttttagccat gcggcgactt catttcctcc aggtacggac
     5941 ccgcgtattt ccatcaacgt gctggagtct gctggactgg aagtgagcca tgtgctggat
     6001 gagccaaccg cagttgcgga tctgctgcaa ctggacaacg ccggtgtagt ggatatcggc
     6061 ggcggcacta ccggcattgc catcgtgaaa aagggcaagg tgacgtactc ggcggatgaa
     6121 gcaaccggcg gacatcacat ctctctgact ctcgccggaa atcgccgtat ttcgctggaa
     6181 gaggcggagc agtacaagcg cggtcacggt gaagagattt ggcctacggt gaaaccggtg
     6241 tacgaaaaaa tggcggacat tgtcgcccgg catattgaag ggcagggcat tactgattta
     6301 tggctggcgg gcggctcttg tatgcaaccg ggcgtggcgg aactgtttcg caaacaattc
     6361 ccggcgttac aggtgcattt accgcagcac agtttgttta tgaccccgct ggcgatcgcc
     6421 agtagtgggc gagagaaagc ggaggggcta tatgcaaaat gaattgcaga ccgcgctctt
     6481 tcaggcgttc gataccctga atctgcaacg ggtaaaaaca tttagcgttc caccggtgac
     6541 gctttgcggt ccgggctcgg tgagcagttg cggacagcaa gcgcaaacgc gtgggctgaa
     6601 acatctgttc gtgatggcag acagcttttt gcatcaggca gggatgaccg ccgggctgac
     6661 gcgcagcctg gctgttaaag gcatcgccat gacgctctgg ccatgtccgg tgggcgaacc
     6721 gtgcatcacc gacgtgtgtg cagccgtggc gcagttgcgt gagtcaggct gtgatggggt
     6781 gatcgcattt ggcggcggct cggtgctgga tgcggcgaaa gccgtggcgt tgctggtgac
     6841 gaacccggat agcacgctgg cagagatgtc agaaaccagc gttctgcaac cgcgcttgcc
     6901 gctgattgcc attccaacga ccgccggaac cggctctgaa accaccaatg taacggtgat
     6961 tatcgacgcg gtgagcgggc gcaagcaggt gttagcccat gcctcgctga tgccggatgt
     7021 ggcgattctc gacgccgcgt tgaccgaagg tgtgccgtcg catgtcacgg cgatgaccgg
     7081 cattgatgcg ttaacccatg ccattgaagc atacagcgcc ctgaacgcta caccgtttac
     7141 cgacagtctg gcgattggtg ccattgcgat gattggcaaa tcgctgccga aagcggtggg
     7201 ctacggtcac gaccttgccg cgcgcgagag catgttgctg gcttcatgta tggcgggaat
     7261 ggcgttttcc agtgcgggtc ttgggttgtg ccacgcgatg gcgcatcagc cgggcgcggc
     7321 gctgcatatt ccacacggtc tggcgaacgc catgttgctg ccaacggtga tggaatttaa
     7381 ccggatggtt tgtcgtgaac gctttagtca gattggtcgg gcactgcgaa ctaaaaaatc
     7441 cgacgatcgt gacgctatta acgcggtaag tgagctgatt gcggaagttg ggattggtaa
     7501 acgactgggc gatgttggtg cgacatctgc gcattacggc gcatgggcgc aggccgcgct
     7561 ggaagatatt tgtctgcgca gtaacccgcg taccgccagc ctggaacaga ttgtcggact
     7621 gtacgcggcg gcgcaataaa tgccggatgc gacgcttgcc gcgtcttatc cagcctacgg
     7681 gattgcacat gtagggcgga taaggcgttc acgccgcatc cgccagtggc gcggtgcaat
     7741 tgccggatgc gatgcttgcc gcgtcttatc cggcctacgg gattgcacat gtagggcgga
     7801 taaggcgttc acgccgcatc cgccagtggc gcggtgcaat tgccggatgc gacgcttgcc
     7861 gcgtctgatc cggccttcgg gattgcacat gtagggcgga taaggcgttt acgccgcatc
     7921 cgccaataaa taacaactaa cagggagtaa aggcgatggg aattaacgaa atcatcatgt
     7981 acatcatgat gttctttatg ctgatagctg ccgtagacag gatcctgtcg cagttcggcg
     8041 gttctgctcg tttcctcggt aagttcggta aaagtatcga aggatcaggc ggtcagttcg
     8101 aagaaggctt tatggcaatg ggcgctctgg gtctggcgat ggtcggtatg accgcgctgg
     8161 caccggtact ggctcacgta ctcgggccgg taattatccc ggtttacgaa atgctcggcg
     8221 caaacccgtc aatgttcgcc ggaacgctgc tggcgtgcga tatgggcggc ttcttcctcg
     8281 ccaaagagct ggcgggcggc gacgtagcag cgtggctata ctctgggtta attcttgggt
     8341 cgatgatggg gccaacgatt gtgttttcca ttccggtggc gctcggcatt atcgaacctt
     8401 ctgaccgtcg ttatctggcg ctcggcgtgc tggcgggcat tgtgaccatt ccgattggct
     8461 gtattgctgg tggtctggtt gctatgtact ccggtgtgca gatcaacggt cagccggtgg
     8521 aattcacttt cgccctgatc ctgatgaaca tgatcccggt gatcattgtt gcgattctgg
     8581 tggcgctggg gctgaaattc atcccggaaa aaatgatcaa cggcttccag atcttcgcca
     8641 aattcctcgt tgcattgatc accctcggtc ttgctgccgc ggtagttaag tttctgcttg
     8701 gctgggaact gatccccggt ctggacccta tctttatggc ccctggcgat aaacccggtg
     8761 aagtgatgcg cgccattgaa gttatcggtt ctatctcctg cgttctgtta ggggcgtatc
     8821 caatggtgct gctgctgacc cgctggtttg aaaaaccgct gatgagcgtc ggtaaagtgc
     8881 tgaatatgaa caacatcgcg gcagccggca tggtggcaac gcttgccaac aacatcccga
     8941 tgttcggcat gatgaagcag atggataccc gcggcaaagt catcaactgc gccttcgccg
     9001 tttccgctgc tttcgccctg ggcgaccact taggcttcgc cgctgccaac atgaacgcca
     9061 tgatcttccc gatgattgtc ggcaagttga tcggcggcgt aacggcgatt ggcgtggcga
     9121 tgatgctggt gccaaaagaa gacgcgacca cggctaaaac cgaagcggag gcacaatcgt
     9181 gaacactcgc cagctattga gcgtcggtat cgatatcggc accaccacca cccaggtgat
     9241 tttctcccac ctggagctgg ttaaccgtgc ggcggtgtcg caggtgccgc gctacgaatt
     9301 cattaaacgc gaaattagct ggcaaagccc ggtgttcttt acccctgtcg ataaacaggg
     9361 tggtttaaaa gaagcggaac tgaaaacctt aatcctcgag caatatcagg ctgcgggcat
     9421 tgcgccggaa agcgttgatt ctggtgccat catcatcacc ggtgaaagcg cgaaaacccg
     9481 caatgctcgt ccggcggtga tggtgctctc ccagtcgctg ggcgattttg tcgttgccag
     9541 cgccgggccg cacctcgaat ccgtcattgc cggtcacggt gccggggcac aaaccctttc
     9601 tgaacaacgg ctgtgtcggg tactgaatat tgacatcggc ggtggcactg cgaactacgc
     9661 cctgttcgat gccggaaaaa tcagcggcac tgcctgcctc aacgtcggtg gtcgcctgct
     9721 ggaaaccgac agccaggggc gcgtggttta cgctcataaa ccggggcaga tgattgtgga
     9781 tgagtgcttc ggcgcaggca ccgacgcccg ttcgctgacc ggcgcgcagc tggtgcaggt
     9841 tacccggcgg atggcagaac tgattgtcga agtgattgac ggaacgctct cgccgctcgc
     9901 gcaggcattg atgcaaaccg gtttgctgcc cgcaggtgtt acgcccgaaa tcattacgct
     9961 ttctggcggc gtgggcgaat gttatcgcca ccagcccgcc gacccgttct gttttgccga
    10021 tattggcccg ctgctggcaa cggcgctgca tgaccatccg cgcctgcgtg agatgaacgt
    10081 acagtttccg gcgcaaaccg tgcgcgccac ggtgattggc gcgggcgcgc ataccctttc
    10141 gctctctggc agcacaatct ggcttgaggg cgtacaactg ccgctgcgca atttgccggt
    10201 ggcgatcccg attgatgaaa cggatctggt gagtgcctgg caacaggcgc tgcttcagct
    10261 ggatcttgat cccaaaactg acgcgtacgt gctggcgctt cccgcctcgc tgcctgtgcg
    10321 ttacgccgcg gtactgacgg tcatcaacgc gctggtcgat ttcgtcgcgc gttttccaaa
    10381 tccgcatccc ctgctggtgg tggccgggca ggactttggt aaagctctgg gcatgttgtt
    10441 gcgcccacag ctacaacaac tcccgttggc agtcattgac gaagtgattg tccgcgcggg
    10501 ggactatatc gacattggta cgcctctttt tggcggatcg gttgtgccgg tgacggtgaa
    10561 atcactcgca tttccttcct gagggaacga cttatgaaac taaagaccac attgttcggc
    10621 aatgtatatc agtttaagga tgtaaaagag gtgctggcta aagccaacga actgcgttcg
    10681 ggggatgtgc tggcgggcgt tgcagcggca agctcacagg agcgcgtggc ggcaaagcag
    10741 gtgttgtcgg aaatgaccgt agcggacatc cgcaataatc cggtgattgc ctatgaagat
    10801 gactgcgtga cgcggctgat tcaggacgat gttaacgaaa cggcctacaa ccagattaaa
    10861 aacaggagca tcagcgaact gcgtgagtat gtgctgagcg atgaaaccag cgtggacgac
    10921 attgccttta cccgcaaagg gctgacctcg gaagtggtcg cggcggtagc gaagatttgc
    10981 tccaacgcgg acctgatcta cggcgcgaag aaaatgccgg taatcaaaaa ggccaatacc
    11041 accatcggta ttccgggcac cttcagcgcc cgtttgcagc caaatgacac ccgtgacgac
    11101 gtgcaaagta tcgctgcaca aatctacgaa gggctttcct tcggggtggg cgatgcggtg
    11161 atcggcgtta acccggtaac tgacgacgtg gaaaacttaa gccgcgtgtt ggacaccatt
    11221 tacggcgtga tcgacaaatt caacatccca actcagggct gcgtactggc gcacgtcacc
    11281 acccagatcg aagcgattcg tcgcggcgcg cctggcggac tgattttcca gagtatctgt
    11341 ggcagcgaaa aagggctgaa agagtttggc gtggaactgg cgatgctcga cgaagcgcgc
    11401 gcagtgggtg ctgagttcaa ccgtatcgcc ggggaaaact gcctctactt cgaaaccggg
    11461 caaggttcgg cgctgtctgc tggcgctaac ttcggcgcag accaggtgac gatggaagca
    11521 cgtaactatg ggctggcgcg tcattacgat ccgtttatcg tcaacaccgt ggtcggcttt
    11581 attgggccgg agtatctcta caacgaccgc cagattatcc gtgctggctt agaagatcac
    11641 tttatgggca agctgagcgg catctctatg ggctgtgact gctgttacac caaccacgct
    11701 gacgctgacc agaacctcaa cgaaaacctg atgatcctgc tcgccaccgc aggctgcaac
    11761 tacatcatgg ggatgccgct gggcgatgac atcatgctca actatcagac caccgcattc
    11821 cacgacactg ccactgtgcg tcagttactc aacctgcgcc cgtcaccgga gtttgaacgc
    11881 tggctggaaa gcatgggcat tatggcaaac ggtcgcctga ccaaacgggc gggcgatccg
    11941 tcactgttct tctgatgacg cggagataac acatcatgga tcaaaaacag attgaagaaa
    12001 ttgtacgcag cgtgatggcg tcaatgggac aagcggcccc cgcgccgtca gaagcaaagt
    12061 gcgccaccac taactgtgcg gcaccggtga cctcggaaag ctgcgcgctg gatttaggtt
    12121 ctgcggaagc aaaagcgtgg attggtgttg aaaacccgca tcgcgcagac gtattaacag
    12181 aactgcgccg cagcaccgtg gcccgcgttt gtaccggtcg taccggtccg cgtccacgta
    12241 cgcaggcgct gctgcgtttc ctggccgatc actctcgttc gaaagatacc gtactgaaag
    12301 aagtgccgga agagtgggtg aaagcgcagg gcttgctgga agtacgctcc gaaattagcg
    12361 acaaaaatct ctacctgact cgcccggata tgggccgccg cctgtgtgca gaagctgttg
    12421 aagcgctgaa agcacagtgt gttgctaatc cggacgtaca ggtcgttatt tctgatggcc
    12481 tgtcaactga tgccatcacc gtgaactacg aagagatcct gccgccgctg atggcgggcc
    12541 tgaaacaggc cgggctgaaa gtcggcacgc cattctttgt tcgttatggt cgcgtgaaga
    12601 ttgaagatca gatcggtgag atcctcggcg cgaaagtggt gatcctgctg gtgggggaac
    12661 gtccggggtt gggtcagtca gaaagcctct cctgttacgc cgtttactcg ccgcgtatgg
    12721 cgaccaccgt tgaggccgat cgcacctgca tctctaacat tcaccagggc ggcacgccgc
    12781 cggttgaagc cgcagccgtc attgtggatt tggcgaaacg tatgctggag cagaaagcat
    12841 ccggcatcaa catgacccga taaggaggca tcatgccagc tttagatttg attcgaccgt
    12901 cggtaaccgc catgcgggtg attgcctccg ttaacgctga atttgcgcgt gaactaaaat
    12961 tgccgccgca tattcgtagc ctcgggctga tttctgctga ttccgatgac gtcacctaca
    13021 ttgcggctga cgaagcgacc aagcaggcga tggtcgaagt ggtatatggc cgctcgctgt
    13081 atgcgggagc agcacacgga ccgtcaccga ctgccggtga agtgctgatt atgcttggtg
    13141 ggccaaaccc ggcggaagtg cgtgctggtc tggatgcgat ggttgcgcat attgaaaatg
    13201 gcgctgcttt ccagtgggct aatgacgcag aaaacaccgc attcctggca catgtagttt
    13261 cgcgtaccgg ttcttatctt tcatcaaccg ccgggatcac gcttggcgat ccgatggcgt
    13321 atctggtggc accgccgctg gaagcgacct acggcattga tgcagcgttg aaatctgccg
    13381 acgtacagct ggtgacctat gtcccgccac cgtctgaaac caactactcg gcagcatttt
    13441 taaccggtag ccaggccgcg tgtaaagcag cctgtaacgc ctttaccgat gcagtgctgg
    13501 aaatcgcgcg taatccaatc cagcgtgcgt aacggaggtt gccgatgatc aatgcactgg
    13561 gattgctgga agtggacggc atggtcgccg cgatagatgc agcggatgcc atgctcaaag
    13621 cagctaacgt tcgtctgctc agtcacgaag tgcttgaccc tggtcgctta acgctggtgg
    13681 tggaaggcga tctggcggcg tgtcgtgcgg cgctggacgc aggttgtgct gccgcgatgc
    13741 gtaccgggcg tgtcatcagc cgcaaggaga tcggtcggcc agacgatgac acccagtggc
    13801 tggttactgg ctttaaccgc cagccgaagc aacccgtaaa ggaacccgac gcgccagtta
    13861 tcgtcgcgga atctgctgac gagttgttgg cgctgttaac atcagtacgt cagggaatga
    13921 cggcaggaga agtggctgcc cactttggct ggccgctgga aaaagccaga aatgcgctcg
    13981 aacagctctt ttctgccggg acgttacgta aacgcagtag tcgttatcgt ctcaagcccc
    14041 attaacctgt cggaggtgcc gggtgtccta caacacccgg cattaacatc atgaaaaaga
    14101 cccgtacagc caatttgcac catctttatc atgaaccctt acccgaaaac ctgaagctca
    14161 cgccgaaggt cgaagtggat aatgttcatc aacgacagac aacggatgtc tatgaacatg
    14221 ctttgacgat taccgcctgg cagcagattt acgatcagct gcatccgggc aagtttcatg
    14281 gtgaatttac ggaaattctg ctcgatgata ttcaggtttt tcgtgaatac accggtctgg
    14341 cgctgcgtca gtcgtgcctg gtctggccga actcgttctg gtttggcatt ccggcgacgc
    14401 gcggtgagca gggatttatc ggttcgcaat gtctgggaag cgcagaaata gcgacgcgcc
    14461 ccggtggaac cgagtttgaa ttaagtacgc cggacgatta tacgatcctg ggcgtagtgc
    14521 tgtctgaaga tgtcatcacc cgtcaggcta actttttgca caatccggat cgggtattac
    14581 atatgctgcg tagccagtcg gcgctggaag tgaaagagca gcataaagcc gcgctgtggg
    14641 gctttgtcca acaggcgctg gcgacgtttt gcgagaaccc ggaaaatctc catcagcctg
    14701 cagtgcgaaa agtgctgggg gataatttgc taatggcgat gggggcgatg ctggaagacg
    14761 cgcaaccaat ggtgacggcg gaaagcatca gtcatcagag ttaccgtaga ttactttccc
    14821 gcgcccgtga atatgtgctg gaaaatatgt ctgagctggt gacggtgctg gacttgtgta
    14881 atcaactgca tgtcagtcgc cgcacgctac aaaacgcgtt tcacgctatt ttaggcattg
    14941 gcccaaacgc gtggctgaaa cgcattcgcc tgaacgccgt acgccgcgaa ctgataagcc
    15001 cgtggtcgca aagcacaacg gtaaaagacg ccgccatgca gtggggattc tggcatctgg
    15061 ggcaatttgc cacggattac cagcagctgt ttgccgagaa gccgtcgttg acgttgcatc
    15121 agcggatgcg ggaatggggg tgagggagtt acacccaatc cctgacctta ataaactcac
    15181 ttaacgccgc ttctgggctg ccatcttttg gctgataatc atactcccag cgtaccagtg
    15241 gcggcattga catcaggatc gactcggtgc gcccgccagt ttgcaggcca aacagagtgc
    15301 cgcgatccca gaccagattg aactcgacat aacgaccgcg acggtagagc tgaaaattgc
    15361 gctcgcgctc gccgtaggcc atcgctttac gtcgctctac aattggtaaa taagcgtcgg
    15421 tgtagccttt gcctaccgcc tgcataaagg caaaacagtg gtcgaaatct ggcgtgttca
    15481 gatcatcaaa gaacagcccg ccaataccgc gctgttcgtt gcgatgtttg aggtagaagt
    15541 aatcgtcgca ccactttttg taacggggat aaacgtcttc accaaatggc aggcacaggt
    15601 cacgggcggt gcggtgccag tgaatggcgt cttcttcaaa accatagaaa ggggttaaat
    15661 cgaagccgcc gccaaaccac cagacgggct ctgcacccgg tttttcggca ataaaaaaac
    15721 gcacattcgc gtggctggtg ggaacatacg ggttatgcgg atgcactacc agtgaaacgc
    15781 ccatcgcctc gaaactgcgc ccggcaagtt ccgggcgatg agcggtggcg gaagcaggca
    15841 tcgcctcgcc gtggacgtga gaaaagttga cgcctgcctg ttcgaaaaca ccaccattac
    15901 gcaacacccg actacgcccg ccgccgccag cttcgcgctg ccaactatct tcgacaaatt
    15961 ctgcgccatc gacggcggtc agctgctgac aaatcgtatc ctgaaggttg agcagaaact
    16021 gtttaacctg gtgtgcgtcg ggtttcataa cttatcgctt tttcgaatgt gctttctggt
    16081 tgtcgaacca gtggaaataa ctgatcacgc cttcagcaat cgctgtggcg attttctgac
    16141 gaaacgccgc cgtgcctaac agccgctctt cttccgggtt ggtgataaac gaggtttcca
    16201 ccagcaccga aggaaccgac ggcgatttca acaccacaaa cgcggcttgt tcggtgttgc
    16261 ggctgtgcag tttatgcacc ggcttaatct ttttcagaat atgcgagcca agcgtcaggc
    16321 tgtttttaat agtatcggtt tgcaccagat caaacagcac ttgctgcaat aggtgatcct
    16381 tgtcagtcgc ctttttaccg gcaacttcat cggcgcggtt ttcgcgttca gacaggtatt
    16441 tcgccattgc gctacttgcc ccacggttag agagggcaaa taccgaagca ccggcagctt
    16501 tcgggttggt aaagccatcg gcatgaattg acataaacag atctgcgcca tgtttatggg
    16561 cgatttcaac gcgatcgtaa agtgggataa acgtatcgcc agaacgcgtt aaacgcgcat
    16621 caatcccatg attacgcaaa atggaacgga cgtttttagc aatcgccagc accacatgtt
    16681 tttctttcga accgttgcgt ccgatcgctc cggtatcaat tccgccgtgg cctggatcga
    16741 gaacaacgac acgtttgccg ccagattttt tggctttcgg cttgctgtgt ccgttgctgg
    16801 tttttaaagg ttcgtctttg gcgatggctt gcgacattcc tggcaacgtc agggcagcca
    16861 aaccggcttt cagcacctgg cggcgcgaag tgagtgtttt tagtggttta aaagtgctca
    16921 tacggcctga gttgtaataa taaagttcca gatgttatat cgtatcgcgt aatccgttac
    16981 gacggtttga tgttgagaat tgttttactt ttcatttcaa tagttgacaa ctccccatta
    17041 tgccactttt tcaaccgatt ttcgtcagat attggctaat tcgaccgttc acgccataat
    17101 cagcctttta acccgcaaaa aggtaacgaa taccatggag atacgcgtat ttcgccagga
    17161 agatttcgaa gaggtcatca ccctttggga gcgttgcgac ttgctgcgtc cgtggaacga
    17221 tccggaaatg gacatcgagc gtaagatgaa ccatgacgtc agtttgtttt tggtcgctga
    17281 ggtaaacggt gaagtggtcg gaacggtgat gggcggttat gacgggcatc gcgggtctgc
    17341 ttattatctt ggcgtgcatc cagagtttcg tgggcgtggg attgccaatg cgttgcttaa
    17401 tcggctggag aaaaagctga ttgctcgtgg ctgcccgaaa attcagatca atgtgccgga
    17461 agataacgac atggtgctcg gaatgtatga acgcctggga tatgaacacg ccgacgtgct
    17521 gagtctgggt aagcgtttga ttgaagatga agagtactga gttccatcct gtccattatg
    17581 atgcgcacgg acgcctgcgt ttacccttgc tcttctggct tgtgctattg cttcaggcgc
    17641 gaacctgggt gctgtttgtc attgccggtg cgtcgcgcga gcagggtact gcgctgctaa
    17701 atctgtttta tcccgatcac gataatttct ggctggggct gattccaggc attccggcgg
    17761 tgctggcgtt tttgttgagc ggtcggcggg ctacatttcc tcgcacttgg catgtgctct
    17821 attttctgtt gctgttggcg caggtggttt tactttgctg gcaaccgtgg ctgtggctga
    17881 acggtgaatc cgttagcggt atcggtctgg cgctggtagt ggcggatatt gtggcgttaa
    17941 tctggctgct gaccaatcga cgtttacgcg cttgttttaa tgaagaaaaa gaataacggc
    18001 actttttggt gaatttgcac tccaagcaac gttattgaat aaccaaagga agtgacatga
    18061 aatcgctgcg tttaatgtta tgcgctatgc cgttgatgct gaccggctgt tccacgatgt
    18121 cgtcagttaa ctggtcggcc gctaacccgt ggaactggtt tggatcgtcc accaaagtga
    18181 gcgagcaggg cgtgggtgaa ttaacggcgt ccacaccact gcaagaacaa gccattgctg
    18241 atgcgcttga tggcgattat cgcctgcgca gcggaatgaa aaccgcgaac ggcaacgtgg
    18301 tgcgcttttt tgaagtgatg aaaggcgaca acgtggcgat ggtgattaac ggcgatcaag
    18361 gcacgatcag tcgcattgac gtgctggata gcgatattcc tgctgacacc ggtgttaaaa
    18421 tcggtacacc gtttagcgac ctttacagca aagcatttgg caattgccaa aaagctgatg
    18481 gtgatgataa tcgtgccgtc gaatgtaaag ccgaaggcag tcaacatatt agctaccagt
    18541 tcagcgggga atggcgtggt cctgaagggt taatgccttc ggacgatacc ctgaaaaact
    18601 ggaaagtcag taaaattatc tggcggcgtt aatttgcgtc tgaacaaacc gccgccgcga
    18661 aaaaacacgt aaaatagcgc tcaacaatgc cacggattgc gtggcattct tattttcagg
    18721 aggaacaatg tctcaggttc agagtggcat tttgccagaa cattgccgcg cggcgatttg
    18781 gatcgaagcc aacgtgaaag gggaagttga cgccctgcgt gcggccagta aaacatttgc
    18841 cgacaaactg gcaacttttg aagcgaaatt cccggacgcg catcttggtg cggtggttgc
    18901 ctttggtaac aacacctggc gcgctctgag cggcggcgtt ggggcagaag agctgaaaga
    18961 ttttccgggc tacggtaaag gccttgcgcc gaccacccag ttcgatgtgt tgatccacat
    19021 tctttctctg cgtcacgacg taaacttctc tgtcgcccag gcggcgatgg aagcctttgg
    19081 tgactgcatt gaagtgaaag aagagatcca cggcttccgt tgggtggaag agcgtgacct
    19141 gagcggcttt gttgacggta cagaaaaccc ggcgggtgaa gagacgcgtc gcgaagtggc
    19201 ggttatcaaa gacggcgtgg atgccggcgg cagctatgtg tttgtgcagc gttgggagca
    19261 caacctgaag cagctcaacc ggatgagcgt tcacgatcag gagatgatga tcgggcgcac
    19321 caaagaggcc aacgaagaga ttgacggcga cgaacgtccg gaaacctctc acctcacccg
    19381 cgttgatctg aaagaagatg gcaaagggct gaagattgtt cgccagagcc tgccgtacgg
    19441 caccgccagt ggcactcacg gtctgtactt ctgcgcctac tgcgcgcgtc tgcataacat
    19501 tgagcagcaa ctgctgagca tgtttggcga taccgatggt aagcgtgatg cgatgttgcg
    19561 tttcaccaaa ccggtaaccg gcggctatta cttcgcgccg tcgctggaca agttaatggc
    19621 gttgtaagcg tttctgattc gatgattaaa ggccagccag taacaaaact gactggcctt
    19681 tttattactt ttgcttcaac gccgcataca cctgatcaac tacccaacca taagttgcaa
    19741 tcggtaactg accgctttcg aacatattgg gatttttttg tggatcggca actggcgaat
    19801 ggggcttgtt gcttaacatc acaatcgcca tatgattgac cggatcgata acggttactg
    19861 tcccggtcca gccagtgtga ccgtaggttt gcgggcttgc cagcgtgcca aacgtcggcg
    19921 tcatggtggc attaccattc acgcgccagc cgaggccaaa agtggcatct tccttagagc
    19981 tggtggtgaa catcttcacc gtttccgcac tgaacagctg cacatcgcca tagcccccgc
    20041 cgttcagcat cgtttgcatt aacaccgcaa tatcgccggt attggaaaac aaacctgcgt
    20101 gcccggaaac gccgcccatc gaataaaagg ctttttcatc gtgcacctga ccccagagag
    20161 tggaggtgcg gatattcggg aaatggatca cgccatcgcg ggtattgccg ttcaattccg
    20221 tggcggcaat ttgttgcggt ttaaagcctt tcagcagcgg gttaaatacc gtatgtgtca
    20281 ggccgagcgg gcgataaatc gactcttcaa catagcggtc aagcggctga ccggtaaccg
    20341 actcgacgat aaatccaagc agcatataat cgacatcgct gtagatatgt ttgctgccgg
    20401 gctgatattc cagcggcgtg cgcttgatca tctccagcgt ctggccttta tcctgggaat
    20461 ataacgcgcc cgcgaccgct ttattcgggt attgcggatc tgccgggaaa ccgccgctgt
    20521 gatgcagcag gtcagaaatc cgcagggtgt ttttgccttt aatggtgtca tttggactat
    20581 cggcaaaccc cggaatatat ttcgcaatcc gatcgtcagg atgcagtttg ccttcgctca
    20641 tcagcttttg cagggcgaag ttcgtggcgt acattttggt gtttgaggcc aggtcataca
    20701 gcgtcccggt ggtggctttg acaggctgtt ccatcaacac gctgccatcg tactttttcg
    20761 ccgctcccca ggctttgcga tacacaatct gattatcttt aatgatcagc aggtttacgc
    20821 cgggataacc gacatcaatt tgctggctaa tccagcgatc catctggtta agccgttcga
    20881 cgttaaaccc tgctttctct ggcgagcttt ctgtcagaac agggtatttt gcggcgctga
    20941 cgctacaact aacagccagc agagaaagat agagcattgt ccgtttcatg atatcagtcc
    21001 agattgacgt tacggcagcc aaagagcgtg gtgaaaataa acccgcaaac ccatgccacc
    21061 agaatcccgc cagcataaac cgccatggcc ggaaggatgc cttgtgcgga agtcatcagc
    21121 ggcagcgcta ccagaccaga cggcccaaag gcgctgttta agcccatcgg cagaccccat
    21181 caggctatca ggccgataaa caaaccgccc gccgcgccgc ctaaacaggc ggtaacaaac
    21241 ggcttcatgc ggggcagggt gacaccgtaa atcagcggtt caccaacgcc cagcaggccg
    21301 ggaataatcg ccccgcgtac ctgactgcgt agcgcactgt gcggttgcgc ccgccagtag
    21361 agtgccagcg ccgcgcccac ctggcccgcg cctgccattg aaaggatggg aaataagctg
    21421 ttgaatccct ggctgtccat taacgcgagg taaacaggaa taaagccctg atgcacgcca
    21481 aataccacgg cgatcaggaa cagcccggct aaaaccgcac aaccgaacgg attactgttc
    21541 aggtgcataa acagccatga cataccttcg aataaccagc cacccagcgg cataatgatc
    21601 aggtacgcaa gcgtagcggt gatcagcagg gtgattaacg aggtcagcag catgtcgaga
    21661 tcgtccgaca taaagcggcg caccatgcct tcaatgcggg cgcaggccca cgcggcaatc
    21721 agcacgccga taatattgcc gcgcggatcg atgggcagac caaagaaatc gtgaaaaccg
    21781 gcgtagtaac cggtggttgc agcggggtta taaccgagca aaaagagtgc ggcaataatt
    21841 gcgccattga cgcccgtgcc gccaaatgcc tgggcggcgt tatagcccac cagaatcacc
    21901 aggaaagtga acaaaccttt gctgaacacc ttcataaaat tcagcgcgtc gggtagcgtg
    21961 ccctgagcat ctgccggaac gtgcatcacc gtggcaatta acgtcgctat ccccagcagc
    22021 agaccggcgg caataaaacc ggggatcagc ggcgtgaaga tggtggcgaa tttggcgaga
    22081 aattgttgca cgccggaggt ttgtttggct tttaactgac gtttgttctg ggcggcgatt
    22141 tctgcggcat cctgtaccgg tgcctcgccc agcagctcgc tcatcgcttt tgcggcacga
    22201 tgcgctttac caggcccaaa aacgacctgc acctggtcgc tggtcaaaat gacgcccttc
    22261 acgccttcca gcgttttgat attgggatca accagtgaac tgtcatgtac acccagacgc
    22321 aggcgcgtca tacagttacc acaactggcg atatttccca gtccgccgac acgggtaaga
    22381 atggtgttca gaagttcact gctgatctct ttggccatgg gttattcctt gtctaaaacc
    22441 tgacgaataa agccgccgtg ttgatccagg cgttttttag cttctgcggc atcgagattt
    22501 ttcagcacca tcacaatggc cgttttacag ttgcgctcgc aggcaattaa cgccgcttcc
    22561 gcttgttcgg cattacatcc ggtggcgttt ttaacgatat tgacctggcg cacatgcagt
    22621 ttttcgttgg tggcgaccac atcgaccatc aggttgccga acactttgcc ggatttaatc
    22681 atcagcccgg tggaaagcat attgagcacc agtttctgcg ctgtgcctgc tttcattcgc
    22741 gaagaaccag tgaccacttc ggcacctaca atcggtgtaa tggcaaactc ggcggtggtg
    22801 gaaacggcac tccccggatt acaggaaatt cccactgtgc ggcaaccgag ctggcgggcg
    22861 tattccagtc cggcaatcac atacggcgta cgaccgctgg cggcaatacc gaccaccaca
    22921 tcctgtgccg ttaaatttat atttttcaga tcgttaatgc cgccttctcg gctatcttcc
    22981 gtgccttcca ccgcgtgctg aatggcatat tcgccgccag caatcaaacc aaccaccaga
    23041 cccggtttca cgccgtaggt gggcggacat tcgctggcat ccagaatccc cagacgaccg
    23101 gatgttcccg caccgagata aatcagacga ccgccgccgc ttacctgggc gtggataaca
    23161 tcgatcgccg cggcgatatc cggcaggacg cgctcaacgg caagcggtac ggttttatct
    23221 tcatcgttga taatccggca catttccagc gtcgatacgc ggtcaatttc agccgaggcg
    23281 gtgttcgagc cttcagtaat catcttttca agttgcatcg gtggtgacct tactatttac
    23341 gatcaaataa ttgcagatgt atggaatcat atatcccgaa ttgacagact taaaggaatc
    23401 attgagtcta cacttgcaga gtgtgaagag cgtcataaga acttaagcgg aatttttgct
    23461 gccagcccgg gagagtggaa gatgttgtac ctgacgaaaa tacgcaatgc ggaaagtgaa
    23521 tttaccgaga acgagcaaaa aattgctgac tttctgcggg ccaacgtgag tgagctgaaa
    23581 tccgtctctt cgcgcaagat ggcgaaacag ctcggcatca gtcagtcgag cattgtgaaa
    23641 tttgcccaga agctcggcgc gcaaggattt actgaattgc gtatggcgtt aattggcgaa
    23701 tacagtgcca gccgggagaa aacgaatgcc acggcgctgc acctgcacag ttcgattacc
    23761 agtgatgatt cgctggaagt gattgcccgc aaactgaatc gcgaaaaaga gctggcgctg
    23821 gagcaaacct gtgcgttgtt cgactacgct cggctgcaaa aaatcattga ggtcatcagt
    23881 aaagcaccgt ttatccagat aacgggcctg ggcgggtcgg cgctggtggg gcgcgatctg
    23941 tcgttcaaac tgatgaaaat tggctatcgc gtggcctatg aagccgacac acacgtacaa
    24001 gccaccgttt cccaggcgtt gaaaaaaggc gatgtacaaa tcgctatctc ttacagcggc
    24061 agtaaaaaag agattgtctt gtgcgcagaa gcggcacgaa agcagggggc aacggtgatt
    24121 gccattactt ctctggctga ctctccgctg cggcggctgg cgcatttcac gctcgatacc
    24181 gtttccggtg aaaccgaatg gcgtagctcg tcgatgtcta cccgcacggc gcaaaattct
    24241 gttaccgatt tactgtttgt tggtcttgtg caactcaatg atgtggagtc attgaaaatg
    24301 attcagcgca gcagtgagct aacacaacgc ctgaaatgat gcataaagca gcgactggat
    24361 tgagattttc ctgaattagt gagctgatcc gcagcaatat tttgtttatc ctgtattttc
    24421 agagggaatg gagtgtaacg ctctgtatta acaaggagag cattaaaatg ggtaaactca
    24481 cgggcaagac agcactgatt acgggcgcat tgcagggaat tggcgaagga attgccagaa
    24541 cttttgcgcg tcatggcgca aacctaatct tgctggatat ctcccctgag atcgaaaagc
    24601 tggcggacga actgtgtggt cgtggtcatc gctgtacggc ggttgtcgcc gatgtgcgtg
    24661 acccggcgtc ggtagccgca gctatcaaac gcgcgaagga aaaagaaggg cgcattgata
    24721 tcctggtgaa taacgcaggc gtttgtcgtc tgggcagttt cctcgatatg agcgatgacg
    24781 atcgcgattt ccatattgac atcaatatta aaggcgtatg gaacgtcacg aaggcggtgc
    24841 tgccggagat gattgcccgc aaagatggtc gcattgtgat gatgtcttca gtcactggtg
    24901 atatggtggc cgatcctggc gaaacggcgt acgccttaac gaaagcggcg attgttggcc
    24961 tgacaaaatc gctggcggtg gagtacgcgc agtctggtat tcgcgttaac gccatttgcc
    25021 cgggatacgt gcgcacacca atggcggaaa gcattgcccg ccagtcgaac ccggaagatc
    25081 cagagtcggt gctgactgaa atggcgaaag caatcccgct gcgtcgcctc gccgatccgc
    25141 tggaagtcgg cgaactggcg gccttcctcg catcggatga atccagctat ttaaccggta
    25201 cacagaatgt gattgatggc ggcagcacac tgccggagac ggttagcgtc ggtatctgat
    25261 tcacctctgt ttcctccctg catttgtggg gaggatttcg ttttaaaccc ggttcttatt
    25321 cccttttcaa cttccaaatc accaaacggt atataaaacc gttactcctt tcacgtccgt
    25381 tataaatatg atggctatta gaaagtcatt aaatttataa gggtgcgcaa tggccgttaa
    25441 cttactgaaa aagaactcac tcgcgctggt cgcttctctg ctgctggcgg gccatgtaca
    25501 ggcaacggaa ctgctgaaca gttcttatga cgtctcccgc gagctgtttg ccgccctgaa
    25561 cccgccgttt gagcaacaat ggaccaaaga aaacggcggc gacaaactga caataaaaca
    25621 atctcatgcc gggtcatcaa aacaggcgct ggcaattttg cagggcttaa aagcagacgt
    25681 tgtcacttat aaccaggtga ctgacgtaca aatcctgcat gacaaaggca agctgatccc
    25741 ggcggactgg caaacgcgcc tgccaaataa tagctcgcca ttctactcca ccatgggctt
    25801 cctggtgcgt aagggcaacc cgaagaatat ccacgactgg aacgacctgg tgcgctccga
    25861 tgtgaagctg attttcccga acccgaaaac gtcgggtaac gcgcgttaca cctatctggc
    25921 ggcatggggc gcagcggata aagctgacgg tggcgataaa gccaaaaccg aacagtttat
    25981 gacccagttc ctgaaaaacg ttgaagtgtt cgatactggc ggtcgtggcg cgaccaccac
    26041 ttttgccgag cgcggcctgg gcgatgtgct gatcagcttc gagtcggaag tgaacaacat
    26101 ccgtaaacag tatgaagcgc agggttttga agtggtgatt ccgaaaacca acattctggc
    26161 ggaattcccg gtggcgtggg ttgataaaaa cgtgcaggcc aacggtacgg aaaaagccgc
    26221 caaagcctac cttaactggc tctacagccc gcaggcgcaa accatcatca ccgactatta
    26281 ctaccgcgtg aataacccgg aagtcatgga taaactgaaa gacaaattcc cgcagaccga
    26341 gctgttccgc gtggaagaca aatttggctc ctggccggaa gtgatgaaaa cccacttcac
    26401 cagcggcggc gagttagaca agctgttagc ggcggggcgt aattaatgtt tgcagtttca
    26461 tcaagacgcg tgctgccggg ctttacctta agcctcggca ccagtctgct gtttgtgtgc
    26521 ctgattttgc tgctgccgct ctccgcgctg gtgatgcagc tggctcagat gagctgggcg
    26581 cagtactggg aggtgatcac caacccgcag gtagtagcgg cctacaaagt aacgctgctg
    26641 tcagcgtttg tggcatcgat ttttaacggc gtttttggcc tgctgatggc gtggatctta
    26701 acccgctatc gctttccggg ccgcacgctg cttgatgcgc tgatggattt accctttgcg
    26761 ctgccaacgg cagtcgccgg tttaacgcta gcctcgctct tttctgtaaa cggtttttac
    26821 ggtgaatggc tggcgaagtt tgatatcaaa gtcacctata cctggctggg gattgcggtg
    26881 gcgatggcct ttaccagcat tccgtttgtg gtacgtaccg tgcagccggt gctggaagag
    26941 ttaggcccgg aatatgaaga agcggcagaa acgcttggcg caacgcgctg gcagagtttc
    27001 tgcaaagtgg tgctgccgga actttctccg gcgctggtgg cgggcgtggc gctgtcgttt
    27061 acccgtagtc ttggtgaatt tggcgcggtg atttttatcg ccggaaatat cgcgtggaag
    27121 acggaagtga cgtcgctgat gatttttgtg cgcttacagg agtttgatta cccggcagcg
    27181 agcgcgattg cttcggtgat cctcgcggca tctctgctgc tgctgttctc aattaacact
    27241 ctgcaaagtc gctttggtcg gcgtgtggta ggtcattaat ggcggaagtt acccaattga
    27301 agcgttatga cgcgcgcccg attaactggg gcaaatggtt tctgattggc atcgggatgc
    27361 tggtatcggc gttcatcctg ctggtgccga tgatttatat cttcgtacag gcgttcagca
    27421 aagggctgat gccggtttta cagaatctgg ccgatccgga catgctgcac gccatctggc
    27481 tgacggtgat gatcgcgctg attgccgtac cggtaaacct ggtgttcggc attctgctgg
    27541 cctggctggt gacgcgcttt aacttccctg gacgccagtt actgctgacg ctactggaca
    27601 ttccgtttgc cgtatcgccg gtggttgccg gtctggtgta tctgctgttc tacggctcta
    27661 acggcccgct gggcggctgg ctggatgaac ataatctgca aattatgttc tcctggcccg
    27721 gaatggtgct ggtcaccatc tttgtgacgt gtccgtttgt ggtgcgcgag ctggtgccgg
    27781 tgatgttaag ccagggcagc caggaagacg aagcggcgat tttgcttggc gcgtctggct
    27841 ggcagatgtt tcgccgcgtc acattgccga acattcgctg ggcgctgctt tatggcgtgg
    27901 tgttgaccaa cgcccgcgca attggcgagt ttggcgcggt gtcggtggtt tccggctcga
    27961 ttcgcggcga aaccctgtcg ctgccgttac agattgaatt gctggagcag gactacaaca
    28021 ccgtcggctc ctttaccgct gcggcactgt taacgctgat ggcgattatc accctgtttt
    28081 taaaaagtat gttgcagtgg cgcctggaga atcaggaaaa acgcgcacag caggaggaac
    28141 atcatgagca ttgagattgc caatattaag aagtcgtttg gtcgcaccca ggtgctgaac
    28201 gatatctcac tggatattcc ttcaggtcag atggtcgcgt tgctggggcc gtccggttcc
    28261 gggaaaacca cgctgctgcg cattatcgcc gggctggagc atcaaaccag cgggcatatt
    28321 cgcttccacg gcaccgacgt cagccgcctg cacgcgcgcg atcgcaaggt cggtttcgtg
    28381 ttccagcatt acgcgctgtt ccgccatatg acggtattcg acaatatcgc ttttggcctg
    28441 acggtgctgc cgcgtcgcga tcgcccgaat gccgcagcca tcaaagcgaa agtgacaaaa
    28501 ttgctggaga tggtgcagct tgctcatctg gcggatcgtt atccggcaca gctttccggc
    28561 ggccagaaac agcgcgtggc gctggcgcgc gcgctggctg tagaaccgca aattctgctg
    28621 cttgatgaac cgtttggcgc gctggatgcg caggtgcgta aagagctgcg tcgctggctg
    28681 cgtcaactgc atgaagagct gaaattcacc agcgtgttcg tgacccacga ccaggaagaa
    28741 gcgacggaag tagctgatcg tgtagtggtg atgagccagg gcaatattga acaggctgat
    28801 gcgccggagc aggtatggcg cgaaccagcg acccgttttg tgctcgaatt tatgggcgaa
    28861 gtgaaccgcc tgcagggaac cattcgcggc gggcagttcc atgttggcgc acatcgctgg
    28921 ccgctgggct acacacctgc ttatcagggg ccggtggatc tcttcctgcg cccgtgggaa
    28981 gtggatatca gccgccgtac cagtctcgat tcgccgctgc cggtacaggt actggaagcc
    29041 agcccgaaag gtcactacac ccaattagtg gtgcagccgc tggggtggta caacgaaccg
    29101 ctgacggtcg tgatgcatgg cgacgatgcc ccgcagcgtg gcgagcgttt attcgttggt
    29161 ctgcaacatg cgcggctgta taacggcgac gagcgtatcg aaacccgcga tgaggaactt
    29221 gctctcgcac aaagcgcctg ataggttgag tgaatgttaa acgcccggag gcgcttcccg
    29281 cgatccgggc tttttaatgg caaggtttgt aacctgtaga cctgataaga cgcgcaagcg
    29341 tcgcatcagg caacaccacg tatggataga gatcgtgagt acattagaac aaacaatagg
    29401 caatacgcct ctggtgaagt tgcagcgaat ggggccggat aacggcagtg aagtgtggtt
    29461 aaaactggaa ggcaataacc cggcaggttc ggtgaaagat cgtgcggcac tttcgatgat
    29521 cgtcgaggcg gaaaagcgcg gggaaattaa accgggtgat gtcttaatcg aagccaccag
    29581 tggtaacacc ggcattgcgc tggcaatgat tgccgcgctg aaaggctatc gcatgaaatt
    29641 gctgatgccc gacaacatga gccaggaacg ccgtgcggcg atgcgtgctt atggtgcgga
    29701 actgattctt gtcaccaaag agcagggcat ggaaggtgcg cgcgatctgg cgctggagat
    29761 ggcgaatcgt ggcgaaggaa agctgctcga tcagttcaat aatcccgata acccttacgc
    29821 ccattacacc actaccgggc cggaaatctg gcagcaaacc ggcgggcgca ttacccattt
    29881 tgtctccagc atgggaacaa ccggcactat caccggcgtt tcacgcttta tgcgcgaaca
    29941 atccaaacca gtaacgattg ttggcctgca gccggaagaa ggcagcagta ttcccggcat
    30001 tcgccgctgg cctgcggaat atctgccggg gattttcaac gcttctctgg tggatgaggt
    30061 gctggatatt catcagcgcg atgcagaaaa caccatgcgc gaactggcag tgcgggaagg
    30121 aatattctgt ggcgtcagct ccggcggcgc ggttgccgga gcactgcggg tggcaaaagc
    30181 taaccctggt gcggtggtgg tagcgatcat ctgcgatcgt ggcgatcgct acctttccac
    30241 cggggtgttt ggggaagagc attttagtca gggggcgggg atttaagatt attgcgcagg
    30301 caccgtttta tccgtctgcg caattaaggc atgtaaaaaa ggctgtgcgt tctcatcgct
    30361 cttaccgggg atcttcacaa tatacggctt cccggtaatc gatgacgacg aagcaacctt
    30421 atcaataaac tgctcggcag tgtcaatgcg gttacgggta ttgccgagct tcagacgcag
    30481 atgagaaacc gcttcatcgc aggtatgttc atcaccgttg cgcacaaata tcaaatcctt
    30541 tttctgtgct aatccctcca gcatggcgtt gatgcgggct tcttcatgaa cagttaactt
    30601 cgcgtaagcg gggagtgtca ttagtagtgt aatgaccaga caaatgattt tcttcattga
    30661 attaccttat ccttgttttg tgatagccga tttgactgcc agaatcaggt aaagtttcgg
    30721 ttggaatacg atgaggatca tcggcaagga gaaaaaatta aatgagtagt ttgttgttgt
    30781 ttaacgataa gagtagggcg ctgcaggcgg atatcgtcgc cgtgcagtcg caggtagtgt
    30841 acggcagcgt aggcaacagc attgcggtgc ctgctataaa acagaacggc ttgaatgtct
    30901 ttgccgtgcc gacggtattg ctgagcaata cgccgcatta tgacactttc tacggtggtg
    30961 cgattccgga cgaatggttt agcggctatt tgcgtgcgct tcaggagcgt gatgcgctgc
    31021 gccaacttcg tgctgtaacc acgggctata tgggaacggc atcgcaaatc aaaatccttg
    31081 ccgagtggct gactgcgcta cgcaaagacc atcctgacct attgatcatg gtcgatccgg
    31141 tgattggcga tattgatagc ggaatttatg tcaaacctga ccttcccgag gcgtatcgac
    31201 aatatttact gccgctggcg caaggaatta cccccaatat ctttgagctg gaaatcctga
    31261 ccggtaaaaa ttgccgcgat ctcgacagtg ccattgctgc cgcaaaaagt ctgctttcag
    31321 acacattaaa atgggtggtg attaccagcg cctccggtaa tgaagaaaat caggagatgc
    31381 aggtggtggt ggtcagtgcc gacagcgtga atgtcatttc ccattcacgg gtaaaaaccg
    31441 acctgaaagg gactggcgac ctgttttgtg ctcagcttat cagtggtttg ctgaaaggga
    31501 aggcgttaaa cgatgcggtg caccgtgcgg ggttgcgcgt actggaagtg atgcgctaca
    31561 cccagcagca tgagagcgat gaattgattt tgccgccgct ggcggaagca taaaaaatgg
    31621 cgccgatgg
//
LOCUS       DAZRUF010000018        30153 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZRUF010000018 DAZRUF010000000
VERSION     DAZRUF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30153
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.018"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHXOL_000710"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHXOL_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR6807768.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHXOL_000711"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHXOL_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR6807769.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHXOL_000712"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHXOL_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR6807770.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHXOL_000713"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHXOL_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR6807771.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHXOL_000714"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHXOL_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR6807772.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHXOL_000715"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHXOL_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR6807773.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHXOL_000716"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHXOL_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR6807774.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHXOL_000717"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHXOL_000717"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR6807775.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHXOL_000718"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHXOL_000718"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR6807776.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHXOL_000719"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHXOL_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR6807777.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHXOL_000720"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHXOL_000720"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR6807778.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHXOL_000721"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHXOL_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR6807779.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHXOL_000722"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHXOL_000722"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR6807780.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHXOL_000723"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHXOL_000723"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR6807781.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHXOL_000724"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHXOL_000724"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR6807782.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /locus_tag="ACHXOL_000725"
     CDS             complement(18516..18992)
                     /locus_tag="ACHXOL_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HFR6807783.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKLGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="ACHXOL_000726"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="ACHXOL_000726"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR6807784.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="ACHXOL_000727"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="ACHXOL_000727"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR6807785.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="ACHXOL_000728"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="ACHXOL_000728"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR6807786.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="ACHXOL_000729"
     CDS             21688..22278
                     /locus_tag="ACHXOL_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR6807787.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="ACHXOL_000730"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="ACHXOL_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR6807788.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="ACHXOL_000731"
     CDS             22750..23445
                     /locus_tag="ACHXOL_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR6807789.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHXOL_000732"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHXOL_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR6807790.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="ACHXOL_000733"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="ACHXOL_000733"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR6807791.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="ACHXOL_000734"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="ACHXOL_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR6807792.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHXOL_000735"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHXOL_000735"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR6807793.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHXOL_000736"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHXOL_000736"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR6807794.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7057 a         7868 c         8306 g         6922 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agagtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg gcggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca agcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag
    19681 tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagccgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc cagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac ccaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccag gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaactcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc
    30121 gtaatatacg ccacctcgcg acagtgcgct aaa
//
LOCUS       DAZRUF010000019        29548 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZRUF010000019 DAZRUF010000000
VERSION     DAZRUF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.019"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="ACHXOL_000737"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="ACHXOL_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR6807795.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="ACHXOL_000738"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="ACHXOL_000738"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR6807796.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHXOL_000739"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHXOL_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR6807797.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="ACHXOL_000740"
     CDS             complement(3644..4030)
                     /locus_tag="ACHXOL_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR6807798.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHXOL_000741"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHXOL_000741"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR6807799.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHXOL_000742"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHXOL_000742"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR6807800.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHXOL_000743"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHXOL_000743"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR6807801.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHXOL_000744"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHXOL_000744"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR6807802.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHXOL_000745"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHXOL_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR6807803.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="ACHXOL_000746"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="ACHXOL_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR6807804.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHXOL_000747"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHXOL_000747"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR6807805.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="ACHXOL_000748"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="ACHXOL_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR6807806.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="ACHXOL_000749"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="ACHXOL_000749"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR6807807.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="ACHXOL_000750"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="ACHXOL_000750"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR6807808.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHXOL_000751"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHXOL_000751"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR6807809.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="ACHXOL_000752"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="ACHXOL_000752"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR6807810.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="ACHXOL_000753"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="ACHXOL_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR6807811.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="ACHXOL_000754"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="ACHXOL_000754"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR6807812.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHXOL_000755"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHXOL_000755"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR6807813.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHXOL_000756"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHXOL_000756"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR6807814.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHXOL_000757"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHXOL_000757"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR6807815.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHXOL_000758"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHXOL_000758"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR6807816.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHXOL_000759"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHXOL_000759"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR6807817.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHXOL_000760"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHXOL_000760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR6807818.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="ACHXOL_000761"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="ACHXOL_000761"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR6807819.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7160 a         7121 c         8159 g         7108 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cattatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgacaaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcccgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttcttgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAZRUF010000020        29143 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZRUF010000020 DAZRUF010000000
VERSION     DAZRUF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.020"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(319..2010)
                     /gene="fruA"
                     /locus_tag="ACHXOL_000762"
     CDS             complement(319..2010)
                     /gene="fruA"
                     /locus_tag="ACHXOL_000762"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR6807820.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(2027..2965)
                     /gene="fruK"
                     /locus_tag="ACHXOL_000763"
     CDS             complement(2027..2965)
                     /gene="fruK"
                     /locus_tag="ACHXOL_000763"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR6807821.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(2965..4095)
                     /gene="fruB"
                     /locus_tag="ACHXOL_000764"
     CDS             complement(2965..4095)
                     /gene="fruB"
                     /locus_tag="ACHXOL_000764"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR6807822.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4463..5644
                     /gene="setB"
                     /locus_tag="ACHXOL_000765"
     CDS             4463..5644
                     /gene="setB"
                     /locus_tag="ACHXOL_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR6807823.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(5641..5895)
                     /locus_tag="ACHXOL_000766"
     CDS             complement(5641..5895)
                     /locus_tag="ACHXOL_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR6807824.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            6050..6622
                     /gene="yeiP"
                     /locus_tag="ACHXOL_000767"
     CDS             6050..6622
                     /gene="yeiP"
                     /locus_tag="ACHXOL_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR6807825.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6845..8311
                     /locus_tag="ACHXOL_000768"
     CDS             6845..8311
                     /locus_tag="ACHXOL_000768"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR6807826.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8429..9415
                     /gene="yeiR"
                     /locus_tag="ACHXOL_000769"
     CDS             8429..9415
                     /gene="yeiR"
                     /locus_tag="ACHXOL_000769"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR6807827.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9454..10167
                     /gene="lpxT"
                     /locus_tag="ACHXOL_000770"
     CDS             9454..10167
                     /gene="lpxT"
                     /locus_tag="ACHXOL_000770"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR6807828.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10579..11145
                     /gene="mepS"
                     /locus_tag="ACHXOL_000771"
     CDS             10579..11145
                     /gene="mepS"
                     /locus_tag="ACHXOL_000771"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR6807829.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11326..12882
                     /locus_tag="ACHXOL_000772"
     CDS             11326..12882
                     /locus_tag="ACHXOL_000772"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR6807830.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            12964..14778
                     /locus_tag="ACHXOL_000773"
     CDS             12964..14778
                     /locus_tag="ACHXOL_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR6807831.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            14779..15873
                     /locus_tag="ACHXOL_000774"
     CDS             14779..15873
                     /locus_tag="ACHXOL_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR6807832.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            15873..16898
                     /locus_tag="ACHXOL_000775"
     CDS             15873..16898
                     /locus_tag="ACHXOL_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR6807833.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            16900..18489
                     /gene="yejF"
                     /locus_tag="ACHXOL_000776"
     CDS             16900..18489
                     /gene="yejF"
                     /locus_tag="ACHXOL_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR6807834.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVHALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18493..18837)
                     /locus_tag="ACHXOL_000777"
     CDS             complement(18493..18837)
                     /locus_tag="ACHXOL_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR6807835.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19170..20227)
                     /locus_tag="ACHXOL_000778"
                     /pseudo
     CDS             complement(19170..20227)
                     /locus_tag="ACHXOL_000778"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(20318..21013)
                     /gene="rsuA"
                     /locus_tag="ACHXOL_000779"
     CDS             complement(20318..21013)
                     /gene="rsuA"
                     /locus_tag="ACHXOL_000779"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR6807836.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21163..22923
                     /locus_tag="ACHXOL_000780"
     CDS             21163..22923
                     /locus_tag="ACHXOL_000780"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR6807837.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23048..23332
                     /gene="rplY"
                     /locus_tag="ACHXOL_000781"
     CDS             23048..23332
                     /gene="rplY"
                     /locus_tag="ACHXOL_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR6807838.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23471..24478)
                     /gene="yejK"
                     /locus_tag="ACHXOL_000782"
     CDS             complement(23471..24478)
                     /gene="yejK"
                     /locus_tag="ACHXOL_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR6807839.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24660..24887
                     /locus_tag="ACHXOL_000783"
     CDS             24660..24887
                     /locus_tag="ACHXOL_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR6807840.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            24907..26667
                     /gene="yejM"
                     /locus_tag="ACHXOL_000784"
     CDS             24907..26667
                     /gene="yejM"
                     /locus_tag="ACHXOL_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR6807841.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26742..26818
                     /locus_tag="ACHXOL_000785"
     tRNA            26742..26818
                     /locus_tag="ACHXOL_000785"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26776..26778,aa:Pro,seq:ggg)
     gene            complement(26921..29143)
                     /gene="yejO"
                     /locus_tag="ACHXOL_000786"
                     /pseudo
     CDS             complement(26921..29143)
                     /gene="yejO"
                     /locus_tag="ACHXOL_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         7004 a         7637 c         7170 g         7332 t
ORIGIN      
        1 aaaaaacgaa atcggccaga tatgaaggcg gtcacaaagt ttgtctattt atgcagcaac
       61 aaaagagaaa gttgtgatgt gtaacacaca atatggcagg caagaaaaag ggcagagatg
      121 tttctgccct ccagattgtt gacaacgcgc gctttgttca tcctggatgt ggcgtaaacg
      181 ccttatccga cctacaaaag cgcaaaaaat tcaatatatt gcagggttca tgtaggtctg
      241 ataagcatag cgcatcaggc aattttgcgt ttgtcatcat cggggcagag atgtctcatc
      301 cccgataatg aagataaatt atgcgttttt ttctactatt tgtgtttccg ggcgtttcag
      361 gaaggcatag gccaaacccg ccaccagcgt accggcaata attgctacca ggtaacccaa
      421 taccggcgta atagcgccag ggatcagcag aacaaacaga ccaccgtgtg gtgccatcag
      481 tttcgcacca atcgccattg agattgcgcc agtcagcgcc ccaccaacga tacagcacgg
      541 tagcacacgc atcggatcac gagcagcaaa cggaattgca ccttccgaaa taaagcacag
      601 accaagtacc agagcggctt tgccaccttc ctgctgcgct ttgtcgaatt tgcgacgcgc
      661 caccattgtt gccagaccca ttgccagcgg tggcaccata cctgccgcca taatcgccgc
      721 catcgggcca taggtttgag tactcagcag acccacaccg aatgcgtacg ctgctttgtt
      781 taccggaccg cccatgtcag tacacatcat gccaccgagg atcgccccca gcagaaccgc
      841 attcgcagtc cccatggtct gcagccagtg agtcaaccca tcgagaatgc cagcaaccgg
      901 tttaccgatt aggtagatca tcgccagacc gaccaccaga ctggaaatta gcggaatgat
      961 caggatcggt ttcagcgcct ccatactctg tggcagtttc aattgcgtac tgattaactt
     1021 cgcaatgtaa ccagccagga agccagcaat tataccgcca ataaagccag aaccggtgct
     1081 gaccgccagc ataccgccaa tcagacccgg tgtgaggccc ggacgatcgg caatggagaa
     1141 ggcaatataa cctgccagta ccggcaccat cagcgcaaag gctgaaccac caccaatctg
     1201 catcagcgcc gctgccaacg tacccggctc tttaaacgct tcgataccaa aagcaaaaga
     1261 aagcgcgata cacagaccac ctgcaacaac catcggcagc atgtaagaga cgcccgtcag
     1321 caagtgacgg taagcgcctg cactctcttt cttaccttca gtggtcgctg tttgagcttt
     1381 gcccgctggt tcatacggcg ttgcttcagc aaccgcttta tccagttcct gcgcagtttt
     1441 cttcagcgcc agaccggtag aggtgcgata catcggttta ccagcaaatt tcgccagatc
     1501 cacttcgata tctgccgcca caatcaccag atccgctgct gcgacttctt caggggtgat
     1561 tgcattgccc gcgccaacag aaccacgggt ttcaactttc acccaccagc cacgtttttt
     1621 cgcttcggtt tcaatggctt cagccgccat aaaggtgtgt gctacgccag tcgggcaagc
     1681 agtcaccgca actacgcgtt tcggaccgct ggcagcaacc ggtgctgtcg cagcgaccgg
     1741 cgcagtgtaa ggtttcgcat ggcctttggc ttcactcagg aacagctcag ggtgcgcaac
     1801 tgcccgggaa atatcaccca gccagacatt tttaccgttc agcgcgctgt cattcgggat
     1861 ggaatcaccg agaacaatcg ccatttctgc gtcgttcgga ttgtcgatga tttccagttt
     1921 tgcttttcgc gccgccgcgc ccagcagggt cttcgccata taggcgcgtg cctgaccgag
     1981 attagcgtca ataatcagca gcgttttcat tatgcctctc ctgctgtcag ttaaaaggtt
     2041 gtaagtcgac gcgcgccatc attgcggcca actgcggacg atcggtaata cccacattgc
     2101 tttgacttac cgccagggct gcaacagctg tcgccagacg cagtgtgtgt tcactggatt
     2161 cacgcatcag caagccataa atcaggccac caaccataga atcccctgcg ccaacggtgc
     2221 ttacgacatc gactgacggt ggtttggcga tccattcgcc ggaggcatta acccaaagcg
     2281 cgccttcggc acccagtgaa ataacaacat gcgcaatacc ttgttcacgt agcgcatgtg
     2341 cagcttcaat cacatctttc atttcaggca gtttacggcc tgcccagatt tccaactcgc
     2401 ggcggttagg tttcaccagc cacggtgccg ctttcaaacc tgctactaac gcttcacggc
     2461 tactatcaaa gataatgcaa ggacactgac tacgcaggcg agtcatccag tcggtgaacg
     2521 cttccgggct gacgcctgac ggtaagcttc cgctgacaca gaccatatcg aactgaccga
     2581 gccagctcag agaatcagtc acaaagcgtt cccagtcggc gagggtgact tcaaaacccg
     2641 agaagttgaa gtcggtcact tcgccgtctt tttccgtcag cttaacgtta attcgggtgc
     2701 gcccctgtac aacctggaaa cggttggcaa tgcccagctc gctgaacagt tgctgaaaac
     2761 catcctgatt gtctttaccc aggaagccgc caacggtgac atcaattccc aggtctttta
     2821 atactttggc cacgttgatg cctttacccg ccgcatgcag accggtggtt ttcaccaggt
     2881 tcacttcgcc gcgttcaatt tccgggcaga aaccaacaag gtcataagcc ggattaaggg
     2941 tgatcgtagc aacacgtctg ctcattatgc gccctcccca agaccagcag cgatagcgtc
     3001 gccaatggct ttcagcgcct gttcagcatc tgcaccctgg gcggtaaagc gtaggcgatg
     3061 acctttctta acgccaagtg ccacaacttt catcagacta cgtccgtttg ccggtttgcc
     3121 ggtgccatca aggtttgtca cggtaatatc actgttaaat tgtttaatgg tattgaccag
     3181 catggtacct ggacgagcat gcaggccgtg ttcattgcgc accacaaact ccgcgcttaa
     3241 cacgtcgtcg gtcggcgcat catcgctggt cagcagcgcc agcaacgttg ccgcatccgc
     3301 tttcagcaag cggtcagctt tattgtcgag caataaatca gcgagacgct taagaaccgc
     3361 gatgggctga tcgtcattca tcgccacact caccagcatg gctgccgttt cgccgtccac
     3421 atcaaaagca tttgccgcac ggcttaccgc aatcgcgcta cgcagattgc cttcggcgct
     3481 atcgctcagc cagataccct gtccgagatt cagcggttgt tcattgatgg ctttggtgac
     3541 gaaagtggcg tcaactgccc ccacctcttt cagacgcgca gcgttcagcg cctgaagagt
     3601 cagcagatcg ctggcgacga tatccagcgt cagcatttcg ttgtcgagct tcagctgctc
     3661 actctgcttt tcgcccatca gtaatgcgcg aagttcttct gctgttgttg ctgacttcag
     3721 ttgttcagca acggaatcat cgctcagtac gtgggtcagc tggcgtagca ggcccagatg
     3781 ttcatccgag ctggcagcaa taccgattgc cacgtacgct acctgaccgt caccccaggt
     3841 gacgccttcc gggaactgaa atacctgaac gccggttttc agcacctgat cgcgggtgtc
     3901 ggtagtgccg tgtggaatag caataccatt gccgaggaac gttgaggttt gctgttcgcg
     3961 cgccagcatg ccattgacgt agccttctgc tacattaccg gcctgcacca gcgccgcagc
     4021 gacctggcga atcgcctctt ctttgtctcc ggccttttcg cccggatgga tgtcctgtac
     4081 ggataactgg aacatagttc tcctctcttg ctgaattgaa acgattcagc ctctatgaga
     4141 aaaaaagcgc caacctggct tagggttaaa gacaagatcg cgctgaaacg tttcaagaaa
     4201 gcataatact tctgtttcag cacgcaagga aagccgtaaa gttatgagca aaagtttgat
     4261 gtgctgcaca attttttgtc atttttccta attgctgatg ggaaaatccg ttatcagcaa
     4321 tttcatttca gcagcgtcag cagccaatca gcaacagtga aatggcattt tgattattcg
     4381 tggcgctttt gatttcgctg tctgtttatt ttctgacaag cagcgtaaac tccgcgtctt
     4441 cctcttccag tgatcgacct gcatgcataa ctcccccgca gtctccagcg cgaaatcgtt
     4501 tgacctgacc tcgacggcgt ttttaatcgt tgcctttctc accggtattg cgggtgctct
     4561 gcaaaccccg acactcagta ttttccttac cgatgaagta catgcccgtc cggcgatggt
     4621 gggattcttc tttaccggca gcgctgtcat tgggattctg gtaagtcagt ttctcgctgg
     4681 gcgctctgat aagcgcggcg atcgcaaatc gctgattgtc ttttgctgcc tgttaggcgt
     4741 gctggcctgc accctttttg cctggaatcg caactacttt gttttgctat tcgttggcgt
     4801 ctttcttagc agctttggct cgaccgctaa cccgcaaatg tttgcccttg cccgtgaaca
     4861 tgccgacaaa accggacgtg aggcggtgat gttcagctct tttttacgcg ctcaggtttc
     4921 actggcatgg gtcattggcc caccgctggc ttatgcgtta gcgatgggtt tcagctttac
     4981 ggtaatgtat ctgagcgcag cggtagcgtt tattgtttgc ggcgtgatgg tgtggctgtt
     5041 tttaccgtcg atgcaaaaag agcttccgct ggcgaccggc acggttgaag cgccgcgccg
     5101 taaccgtcgc gatacgctac tgctgtttgt catttgtaca ttgatgtggg gctcgaacag
     5161 cctgtacatc atcaacatgc cgctatttat tatcaacgaa ctgcatcttc ccgagaaact
     5221 ggccgatgtg atgatgggga ccgccgccgg gctggaaatc ccgacgatgt tgattgccgg
     5281 atatttcgcc aaacgtctgg gtaagcgttt cttaatgcgc gttgctgccg tgggtggcgt
     5341 ctgtttttac gcaggaatgc tgatggcgca ttcacctgtc attctgttgg gcttgcagct
     5401 gctaaatgct atttttattg gcattctggg cggcatcggg atgctctatt ttcaggatct
     5461 gatgcccggt caggcgggtt cagccaccac gctctatacc aacacttcgc gcgtgggctg
     5521 gatcatcgca ggatcagtgg cgggcatcgt cgccgaaatc tggaattatc acgctgtgtt
     5581 ctggtttgcg atggtgatga ttatcgccac tctgttttgc ttactgcgga ttaaagatgt
     5641 ttaaggcgcg gtcagcattt ccagatcgat aagccaggtc atggcctgct gacgcgagtt
     5701 accgcacatt tcagcgctgg cttgtaagcc tgcacagact ttcggtcgca gtggcgaggt
     5761 gaaaatttta cagcgttgct gttcatcaag ctgaatgcag ggcgtattgg cgggcttgcc
     5821 atctggcata ccgggaatag ggctggaaat tgaaggggca gtgcaacacg ccccgcaacc
     5881 cggacggcat tccatacgtt gttctcttaa ggttaaccac cggattgatg agcgcgcaca
     5941 gtaccacctt ttttgcacca gcaaaagtgc gaataccact tgcccgaaag gcccgtcgcg
     6001 agtactttgt cgcgatattt ttgacatttt cgactacagg aatttttcga tgccaagagc
     6061 gaacgaaatt aaaaaaggta tggtactgaa ttacaacggc aaactgctgc tggttaagga
     6121 tattgatatt cagtcgccca ctgcccgcgg tgccgctacg ctgtacaaaa tgcgtttttc
     6181 tgatgtccgt accgggctga aagtagaaga gcgtttcaaa ggtgatgata tcgttgacac
     6241 cgtgacgctg acccgccgtt acgttgactt ctcctatgtc gatggcaacg aatatgtctt
     6301 tatggataaa gaagactata ccccgtatac cttcaccaaa gatcagattg aagaagagtt
     6361 gctgtttatg ccagagggcg gcatgccgga catgcaggtg ctgacctggg acggtcaact
     6421 gctggcgctt gagctgccgc agaccgttga tctggaaatc gttgaaaccg caccaggcat
     6481 caaaggggca tctgccagcg cccgtaacaa accggcgaca ttgagcactg gtctggtgat
     6541 tcaggtacca gaatacttaa gcccgggcga aaaaattcgt atccatatcg aagaacgccg
     6601 ttatatgggg cgtgctgact aacttcagcc gcatgcagaa aagggatagc tcaggctgtc
     6661 ccttttttaa tttattatac caatcttcta ttttgcgctt cctgaacacc ttacgccctc
     6721 aattttcact cgttgattga tcgccctcac acttcatcgc attaacaatc cagaccaatt
     6781 tcaattgctg tcatataact ttacactgtc attgttaatt tatcgttact aagacgtgac
     6841 tcctatgaag acaattgcct ccgttacgct cccgcatcat gtacacgctc cacgctacga
     6901 tcgccagcag ttgcaatcac gtatcgttca ttttggcttt ggcgcctttc accgcgctca
     6961 tcaggcgtta ctgaccgatc gtgtgctgaa tgcccagggc ggcgactggg ggatctgtga
     7021 aatcagcttg ttcagcggtg atcaactgat gagccagctc cgcgcacaga accatttata
     7081 taccgtgctg gagaaaggtg cggacggcaa tcaggtgata attgtcggtg ccgttcacga
     7141 atgccttaat gcaaaactgg attccttagc ggcaattatt gagaaatttt gcgagccaca
     7201 ggtggcaatt gtttccctga cgattaccga aaaaggctat tgtattgacc cggccaccgg
     7261 tgcactcgac accagtaatc cgcggattat tcacgatcta caaacccctg aagaacctca
     7321 ctccgcaccg ggtattctcg tcgaagcact gaaacgccgc cgtgagcgcg gccttacacc
     7381 gtttaccgtg ctctcctgcg acaatattcc cgacaatggc catgtggtga aaaacgcggt
     7441 gctgggaatg gcagaaaaat gttcgccaga actcgccggg tggataaaag aacacgtcag
     7501 ttttccggga accatggtcg accgcattgt tccggctgca accgacgaat cactggcaga
     7561 aatcagccag catctggggg taaatgatcc ctgcgcgatt agctgcgaac cgtttatcca
     7621 gtgggtggtt gaagataact tcatcgctgg gcgtcctgcc tgggaagtcg caggtgtaca
     7681 aatggtgaat gatgtcctgc catgggaaga gatgaaactg cgtatgctta atggcagcca
     7741 ctcttttctc gcttatctgg gttacctctc aggattcgtc catatcagtg attgcatgca
     7801 ggatcgcgta tttcgccatg ctgccagaac attaatgctg gatgagcaag cgccgacact
     7861 gcaaattaaa gatgtcgatt taacacaata tgcggataag ttaattgcac gttttgctaa
     7921 tccgtcgctg aaacataaga cctggcaaat cgcgatggat ggcagccaga aattaccgca
     7981 acgtatgctg gcaggtattc gcatacatct ggggcgtgaa acggactggt cgttgctggc
     8041 attaggcgtt gctggctgga tgcgttacgt cagcggcgtt gatgatgccg gaaatgccat
     8101 tgatgttcgc gatccgctta gcgataaaat tcgcgaactt gttgcgggca gcagcagtga
     8161 acaacgcgta accgccctgc tttccctgcg tgaagttttc ggtgatgatc tgccagataa
     8221 cccgcatttt gtgcaggcca tcgaacaagc ctggcaacaa atcgtacaat tcggcgcaca
     8281 tcaggcgcta ttaaacaccc tcaaaattta acgatttctg cggttaaagc ggatgaagct
     8341 caccttcgtc cgctctcccc ttctcttttc tgcctttttt agccaggatt aacgctcagt
     8401 taacttacca gagtattgcg gagccgttgt gaccaggacc aacctcatca ccggttttct
     8461 cggcagcggg aaaaccacgt cgattcttca tctgttagcc cataaagatc ccaacgaaaa
     8521 atgggcggta ctggttaatg aatttgggga agtcggaatt gatggtgctt tgctcgccga
     8581 tagcggcgca ttgctgaaag agatccccgg cggctgcatg tgctgcgtta atggtttacc
     8641 catgcaggta gggttgaata ccttactgcg tcagggaaaa ccagaccgct tgttgataga
     8701 gccgaccggg ctgggccatc cgaaacagat cctcgatctg ttaaccgcac cagtctatga
     8761 actgtggata gatctgcgcg ccaccttgtg cattctcgat ccacgcctgc tgctggacga
     8821 aaaaagcgcc agcaatgaaa acttccgtga ccagctggct gccgcagaca tcattgtcgc
     8881 caataaatcc gaccgtgcga cgcccgaaag tgagcaagcg ctacagcgtt ggtggcagca
     8941 aaatggtggc gatcgacaat taattcacag tgagcatggg aaagttgacg gtcatcttct
     9001 ggatttgccg cgtcgcaatt tagccgagtt gcccgccagc gccgcgcatt ctcatcagca
     9061 tgtcgtgaaa aaagggttag cagcgttaag cctgccagag catcaacgct ggcgtcgcag
     9121 tctgaacagc gggcaaggat atcaggcctg cggctggata ttcgacgctg atacggtatt
     9181 cgacaccatt ggcattctgg aatgggcgcg acttgcaccg gtggaacgcg tcaaaggcgt
     9241 gctgcgtatt cccgaagggc tggtacgaat caaccgtcag ggcgatgacc tgcacattga
     9301 aacgcaaaac gttgcgccac cggacagccg tattgagctg atttccagca gcgaagctga
     9361 ctggaatgct ttgcagagcg cgctgttgaa gcttcgttta gcgactaccg cgtaaggttg
     9421 cctgcgtttt tcagtaagat aattagagaa aatatgatta aaaatttgcc gcaaatagtg
     9481 ttgttgaata ttgtcggcct cgcgctgttt ctttcctggt atattcccgt taatcatgga
     9541 ttctggttgc cgattgatgc ggatattttt tatttcttta atcagaaact ggtcgaaagt
     9601 aaggcctttt tgtggctggt tgcattgacc aacaatcgcg ctttcgacgg ttgttctctg
     9661 ctggcgatgg gaatgttgat gctgagtttc tggctgaaag aaaacgcccc tggcagacga
     9721 cgtatcgtga ttatgggtct ggtcatgcta ttaactgcag tggtattaaa ccagctgggt
     9781 caggcattaa ttcctgtaaa acgggccagc ccaacattga cttttaccga tattaaccgc
     9841 gtcagcgaac tgctctctgt tcccacgaaa gatgcctcac gagatagctt tcccggcgat
     9901 cacggcatga tgctgcttat tttttcggca ttcatgtggc gttatttcgg caaagttgca
     9961 ggccttatcg cccttattat ttttgtggtt tttgcatttc ccagagtaat gattggcgca
    10021 cactggttta ctgacatcat tgtcggttcg atgaccgtga tattgatcgg tttgccctgg
    10081 gtgttgctga cgccattaag tgatcgatta atcacctttt ttgacaaatc actaccggga
    10141 aaaaacaaac atttccaaaa caaataactc acagtaatta acatcatcag gcttattttt
    10201 atagtgagga taatcctgat gatgcgcacc gtgctttcat ccatcgcgcg caaaaatcat
    10261 tctctaagta aatgaatgga ttgcatgcgt ttcactcaat cgtactttaa ttgaccaatc
    10321 ccgcttatta actttctgta tcactttttc ttataaaaaa tcatgtaaaa ccgctcgcca
    10381 agaccgcacc aatcgggtaa tctcgaactc gttttgcctc ggcggtagat tatcctcaca
    10441 gcatataatt ttgtgcgtta gtccacagat ttggccttaa ggaattgttt caacatgccc
    10501 aggtaattag tctcgtgccg cttggcattt ttttataacg atatttgtcg ttaaggactt
    10561 caagggaaaa caaacaacat ggtcaaatct caaccgattt tgagatatat cttgcgcggg
    10621 atccccgcga ttgcagtagc ggttctgctt tctgcatgta gtgcaaataa caccgcaaag
    10681 aatatgcatc ctgagacacg tgcagtgggt agtgaaacat catcactgca agcttctcag
    10741 gatgaatttg aaaacctggt tcgtaatgtc gacgtaaaat cgcgaattat ggatcagtat
    10801 gctgactgga aaggcgtacg ttatcgtctg ggcggcagca ctaaaaaagg tatcgattgt
    10861 tctggttttg tacaacgtac attccgtgag caatttggct tagaacttcc gcgttcgact
    10921 tacgaacagc aggaaatggg taaatctgtt tcccgcagta atttgcgtac gggtgattta
    10981 gttctgttcc gtgccggttc aacgggacgc catgtcggta tttatatcgg caacaatcag
    11041 tttgtccatg cttccaccag cagtggtgtt attatttcca gcatgaatga accgtactgg
    11101 aagaagcgtt acaacgaagc acgccgggtt ctcagccgca gctaataaac cgtttggatg
    11161 caatcccttg gctatcctga cgagttaact gaaagcactg cttaggcagt gcttttttgt
    11221 tttcattcat cagagaaaat gatgtttccg cgtcttgatc caggctatag tccggtcatt
    11281 gttatctttt aaatgttgtc gtaatttcag gaaattaacg gaatcatgtt catacgcgct
    11341 cccaattctg gacgtaagct cctgcttacc tgcattgtag caggcgtgat gattgcgata
    11401 ctggttagct gccttcagtt tttagtggcc tggcataagc acgaagtcaa atacgacaca
    11461 ctgattatcg acgtacaaaa gtatctcgat acctattttg ccgacctgaa atccactact
    11521 gaccggcttc agccgctgac cttagatacc tgccagcagg ctaaccccga actgaccgct
    11581 cgcgcggcgt ttagcatgaa tgtccgtacg tttgtgctgg tgaaagataa aaaaacattc
    11641 tgttcatctg cgactggtga gatggacatt ccactcaatg aattgattcc ggcgctcgac
    11701 attaataaaa acgtcgatat ggcgatctta cccggcacgc cgatggtgcc gaacaaaccc
    11761 gcaatcgtca tctggtatcg caaccctttg ctgaaaaata gcggtgtgtt tgccgctctg
    11821 aatctcaacc tgacgccttc tctcttttat agttcacggc aggaagatta cgatggcgtc
    11881 gccctcatta ttggcaatac tgcgctatct accttttctt cacgtttgat gaacgttaat
    11941 gaattaaccg acatgccggt ccgtgaaact aaaattgcgg gcattcctct gaccgttcgg
    12001 ctttatgcgg atgactggac atggaacgat gtgtggtacg catttttact gggcggcatg
    12061 agtggaactg tcgttggcct gctctgctat tacctgatga gcgtacgtat gcgccccggc
    12121 agagaaatca tgaccgccat caagcgcgaa caattttacg tggcgtatca accggtggtg
    12181 gatacacaag ctttgcgagt aacgggcctg gaagtactgc tacgctggcg gcatcctgtc
    12241 gcgggagaaa ttcccccgga tgccttcatt aactttgccg aatcgcaaaa gatgattgtg
    12301 ccgctgactc agcacctgtt tgagttaatt gcccgcgatg ccgcagaatt agaaaaagtg
    12361 ctgccagtag gcgtcaaatt tggtattaac attgcgccgg accatctgca cagcgaaagc
    12421 tttaaagcag atatccagaa actgctcact tccctgcccg cacaccattt ccagattgtg
    12481 ctggaaatta ccgagcgcga tatgctgaaa gagcaagaag ccacacaact cttcgcctgg
    12541 ctgcactcgg tcggcgtaga aattgctatt gatgacttcg gcaccgggca cagcgcgctt
    12601 atctatcttg agcgttttac gctcgattat ctgaaaattg accgtggatt tatcaacgcc
    12661 atcggtacgg aaacgatcac ttcacccgta cttgacgcgg tgctgacgct ggcgaaacgc
    12721 ctcaatatgc tgacggttgc tgaaggggtc gaaacgccgg aacaggcgcg atggctaagc
    12781 gaacgcggcg ttaatttcat gcaaggctac tggattagtc gccctttacc gctggacgat
    12841 tttgtccgct ggctgaagaa accgtatacg ccgcagtggt aaggtgtgct tacgtccctt
    12901 attattcata gtgaaagcat gccggattgc ggctaatgat gagtaaaagg aaatccattg
    12961 cagatgattg tgcgcatact gctgctgttt atcgctctgt tcacctttgg tgtgcaggcg
    13021 caggctatca aggaaagcta tgcctttgcc gtgctgggcg aaccccggta cgcgtttaat
    13081 ttcaaccatt ttgattatgt gaaccccgcc gcgccaaaag gtgggcagat aacgttgtca
    13141 gccctcggca ccttcgataa tttcaaccgc tatgcactgc gcggcaaccc gggcgcacgc
    13201 accgagcagc tgtacgacac gctatttacg acttccgatg acgaaccagg cagttattac
    13261 ccgctgattg ctgaaagcgc acgctatgct gacgattatt cctgggtgga ggtcgctatt
    13321 aatccgcgcg cccgttttca tgatggttcg cccattactg cccgcgatgt agagtttact
    13381 tttcaaaaat ttatgaccga aggcgtgccg caatttcgtc tggtctacaa aggcaccacc
    13441 gtcaaagcca ttgcaccgtt aaccgtgcgc attgagttag ctaaacccag caaagaagat
    13501 atgctgagtc tgttttcgct gccgatattt ccagaaaagt actggaagga tcacaaactt
    13561 agcgacccgc tcgccacgcc tccgcttgcc agtggtccgt accgcgttac gtcctggaaa
    13621 atggggcaaa atattgtcta ttcccgcgtg aaagattact gggcagcaaa cttaccggta
    13681 aaccgtggac gctggaattt cgacaccatt cgctacgatt attacctcga tgataatgtc
    13741 gcctttgaag cgtttaaagc aggtgccttt gatttgcgta tggaaaacga cgctaaaaac
    13801 tgggccacgc gttataccgg taaaaatttc gataaaaaat acatcatcaa agatgagcaa
    13861 aagaacgaat cagcccagga tacgcgctgg ctggcgttta atatccaacg tccggtattc
    13921 agcgatcgcc gggtccggga agcaatcact ctcgcctttg actttgaatg gatgaacaag
    13981 gcgttgtttt acaacgcctg gagtcgcacg aacagttatt ttcagaatac cgaatacgcg
    14041 gccagaaatt accccgacgc cgcggagctg gtgcttctgg caccaatgaa aaaagatcta
    14101 ccgccagaag tcttcacaca aatctaccag ccgccggtat ctaaaggcga tggctacgat
    14161 cgtgacaacc tgttaaaagc cgacaaactt ctcaacgaaa cgggctgggt gctgaagggt
    14221 cagcaacgcg ttaatgccac aacgggtcag ccgctcagct ttgaattatt gcttcccgca
    14281 agcagcaata gtcagtgggt attgccgttc cagcacagcc tgcaacggct gggtatcaat
    14341 atggacattc gcaaggtgga taactcgcaa atcactaacc gcatgcgcag tcgcgactat
    14401 gacatgatgc cgcgcctatg gcgggcgatg ccgtggccca gttccgattt acagatttcc
    14461 tggtcatcgg aatatatcaa ttccacttat aatgcccccg gcgtgcaaag cccggttatc
    14521 gactcgctga tcaaccaaat tattgccgcg cagggaaata aagaaaaatt actgccgttg
    14581 gggcgagcac tggatcgcgt attaacgtgg aattattaca tgctgccaat gtggtacatg
    14641 gcggaagacc gtctcgcctg gtgggataaa ttctcccaac ccgctgtacg ccctgtttac
    14701 agcctgggta tcgatacctg gtggtatgac gttaataaag cggccaaact gccgtcagcc
    14761 agacaacagg gagagtagat gggcgcttac ctgattcgcc gtctgttgct ggtgatccca
    14821 acgttatggg ctattatcac tatcaacttt ttcatcgtgc aaattgcgcc tggcggtccg
    14881 gtcgatcagg ccatcgccgc cattgagttt ggtaatgccg gagtattacc cggcgcaggc
    14941 ggtgaaggtg ttcgtgccag ccatgcgcaa acgggtgtcg gcaatatcag cgacagtaat
    15001 taccgtggcg gacgcggatt agacccagaa gtgatcgctg agatcactca tcgctacggt
    15061 ttcgataagc cgatccacga acgttacttc aaaatgctct gggactacat ccgctttgat
    15121 tttggtgata gcctgtttcg cagcgcctcg gtgctgacgc tgattaaaga cagtctgccg
    15181 gtttccatca ccctcggatt gtggagcacg ctgattatct atctggtgtc gattccgtta
    15241 ggcattcgca aagctgttta taatgggagt cgctttgacg tctggagtag cgcatttatc
    15301 atcatcggct acgccattcc ggcctttttg tttgccatcc tgctgattgt cttcttcgcg
    15361 ggcggcagct atttcgacct gttccctcta cgcggcctgg tttccgctaa ctttgattcg
    15421 ctgccgtggt atcagaaaat caccgattat ctgtggcata tcacgctacc ggtgctggcg
    15481 acagtgattg gtggctttgc ggcgctgacc atgctgacca aaaactcatt ccttgatgaa
    15541 gtgcgcaagc aatacgtggt gaccgcgcgt gcgaaagggg taagtgagaa aaacattctc
    15601 tggaaacatg tgttccgcaa cgccatgctg ctggtgattg ccggttttcc ggcgacgttt
    15661 atcagcatgt tttttaccgg ctcgctgctg attgaggtga tgttttcact caatggtctt
    15721 ggcttactgg gctacgaagc gaccgtctcg cgcgattatc ctgtaatgtt tggtaccttg
    15781 tatattttca ccctgattgg cctgctgctg aatattgtca gtgatatcag ctatacgctg
    15841 gttgatccgc gtatagattt tgagggacgt taatgtcgcg actcagcccc gtcaatcagg
    15901 cccgttgggc gcgttttcgt cataaccgtc gcggctactg gtcgttatgg attttcctcg
    15961 ttttgtttgg tttgagtttg tgttctgaac ttatcgctaa cgataaaccg ttgctggtgc
    16021 gttatgacgg cagttggtat ttcccgttgt tgaaaaacta cagcgaaagc gattttggcg
    16081 gcccgctggc aagtcaggct gattatcagg acccgtggct gaaacaacgg ctggaaaata
    16141 acggctgggt actgtgggca ccgattcgct ttggtgctac cagtatcaac tttgctaccg
    16201 ataagccctt cccttctcca ccctcccggc aaaactggct gggaacggat gccaacggcg
    16261 gcgatgtgct ggcacgcatt ctctatggca cgcggatctc ggttctgttt ggcctgatgc
    16321 tgactctctg ttccagcgtg atgggcgtgc tggcgggggc gctacaaggc tattacggcg
    16381 gtaaagtcga tctctgggga caacgcttta ttgaagtatg gtcggggatg ccgacgctgt
    16441 ttttgattat tttactttcc agcgtcgtac agcctaactt ctggtggctg ctggcaatta
    16501 ctgtcttgtt tggctggatg agtctggtcg gcgtggtgcg ggcggagttt ttacgtactc
    16561 gtaatttcga ctacattcgt gcggcacagg cgcttggcgt cagcgatcgc agtatcatcc
    16621 tgcgtcatat gttgcctaat gccatggtcg cgaccctcac ctttttaccg tttattttat
    16681 gtagttcgat aaccaccctg acctcgctcg atttcctcgg cttcggtcta ccgctcggtt
    16741 caccgtcact cggtgaactg ctgttacaag ggaaaaataa ccttcaggcc ccgtggcttg
    16801 ggatcaccgc cttcttgtcg gtggcgatat tattgtcttt gctgatcttt attggtgaag
    16861 ccgtccgcga cgcatttgat cctaataagg cggtgtagca tgacgcaaac tctgttagcg
    16921 attgaaaatt tgtcggtggg ttttcgccat cagcaaaccg tacgtacagt agtcaatgat
    16981 gtttcactac agattgaggc tggcgaaacg ctggcgctgg tgggtgagtc aggttcaggc
    17041 aaaagcgtta ccgcgctgtc aattttacgc ctgctccctt ccccgccggt tgaatatctc
    17101 tccggcgata ttcgttttca tggcgaatcg ctgcttcatg ccagcgatca aacgttgcgc
    17161 ggtgtacgcg gtaataagat cgccatgatt tttcaggaac cgatggtgtc gttaaatcca
    17221 ttgcataccc tggaaaaaca gctttatgaa gtgctttcac tccaccgcgg gatgcgtcgg
    17281 gaagcggctc gtggcgaaat tcttaactgc cttgatcgcg tcggtatccg ccaggcggca
    17341 aaacggctga cagattatcc gcatcagctc tccggcggcg aacgccagcg ggtgatgatt
    17401 gcgatggcgc tgttaacgcg accggaatta ttaattgccg atgaaccgac caccgcactg
    17461 gacgtctctg tccaggcgca gattttacag ctgttgcgcg aactgcaagg cgagctgaat
    17521 atgggcatgc tgtttattac tcataacctc agcattgtca gaaaactggc ccaccgcgtg
    17581 gcggtaatgc aaaacggtcg ctgtgtcgag caaaataacg ccgctacgct atttgcctca
    17641 cccactcatc cttacacaca aaagctactc aacagtgaac cgtcaggcga tccggtaccg
    17701 ttgccagaac ctgcctcaac gttgctggat gttgaacagc ttcaggttgc cttccccatt
    17761 cgcaaaggga ttttgaagcg cattgtggat cataatgtgg tggtgaaaaa catcagtttt
    17821 acgctacggg cgggtgaaac actgggttta gtgggcgagt ctggttccgg gaaaagcacg
    17881 acgggactgg cgctgctgcg actgattacc tctcagggca gcatcgtctt tgacggtcag
    17941 ccactgcaag atttaaatcg ccgccagctg ttacctactc gtcatcgtat tcaggtggta
    18001 tttcaggatc caaactcctc gctcaaccca cgactcaacg ttttgcagat tattgaggaa
    18061 ggcttacggg ttcaccagcc gacgctttct gccgcacaac gcgaacaaca agtgatagcc
    18121 gtgatgcatg aagtgggatt agatcctgaa acacgccacc gttatccggc ggagttctct
    18181 ggtggtcagc gacaacgtat tgcgattgcc agggcattaa ttcttaagcc ctcactgatc
    18241 atacttgatg aaccgacatc atcactcgac aaaacggtac aggcgcaaat attgacgcta
    18301 ttgaaatcat tgcaacaaaa gcatcaactg gcctatttgt ttatcagcca cgatttgcac
    18361 gttgtccacg cgttatgtca tcaggttatc gtactgcgac aaggggaagt agtggaacaa
    18421 ggaccgtgcg cgcgcgtgtt tgccgcaccg cagcaggagt atacgcgtca gctactggcg
    18481 ttgagctgac gcttaaaaag gattgtagtt tgaaaagggt tcggcaattg ccacaccaaa
    18541 atttttcaat cggcatgttg cagcaaactc gtcctggcga tttacaaaca ggcacggctc
    18601 accttcacac tccagcaccg aacatggaac ttcgatgtcg cttaacgcct ggcttagttt
    18661 atacatcacc gaccatgcat tatcaccatc cgggctaagc aatttaagcg ccaccaggct
    18721 gttgtcaccg cacggtccat tttgcggaat cggttcaacc ttcgaacctg cgaaaccagc
    18781 agaccagcga aagttctgcg gtagacgatg gacgattgag agttgtaatg aagtcactta
    18841 ttttccccgg aagcacattt acttcacaat ttatttacat caattttaac acatcatcaa
    18901 caatccgtct ttatacagat cgtaaagatt gatgcctcgc ctgcatggcc tctatggctc
    18961 tgatttaagt aatatctgcg ggcgtggtca cgtttgcggg gctatatgta cccgtttctg
    19021 cgatctaact caaccttttt aactacaatg atgtgacttt ttacataaat tgattttaca
    19081 taaaataaac atatatcggg ggaatgatag atttgtggtt gacatgcatc aacaaaggaa
    19141 gccttttagc ttcctcgttg tgcaatagat caccgttttt tcggtcgact ggcgtacaga
    19201 cagaagagaa tggagctggt tgcgcagaat gcaattgacc aaatcatcgg ccatgcagag
    19261 ttaaaggtcg caagagaaag caatgcgcca acaattgccc ctatgccaaa acggaaggtt
    19321 cctgccagcg aagatgccgt tcccgccata tggggaaact catcaagaat gaccgccatc
    19381 gcattggatg acaccataga cacgcagccc acaaacgccg caacgccaac caccagcgac
    19441 cagaatccca gccccagcag cgcactgatg accatccacg ctgccataat aaattgtatc
    19501 cacaaccccg agcggaacat atttaacgcg ccaatgcggc ggacgaagcg gctgttaaag
    19561 atggtcatca cgaacagaaa aacaatgttt agcgcaaagt aataaccaaa gttttccggt
    19621 gcaacgtggt tgatttcaat gtagacaaac ggtccggcgc tcaggaacga gaacattccg
    19681 gcaaagctga aaccgctggc cagcatataa ctcaggacac gcttatgacg gaacagcgcc
    19741 gcaaagttac caatagtggt acgaatgtga aaaggctggc gaggatccag aagatgtaat
    19801 gccagctcag ccacaccagc acccagccgc caactatcgg tgccatcagc ggtgcaatgg
    19861 tcgtcaccag catgacaaac gacatcatcc gcgagaactc ttctttcggg taaatatcac
    19921 gcatcaaagc gttgataacc acgctggccg cagccgcagc cagcccgtgg aagaaacgca
    19981 tcacaatcag ctgatcgatg gtttgtgcca acgcacacgc caccgcggcg gcggcaaaca
    20041 ccagcgtacc gccaagaacc accggcttac gcccgaagct gtctgccatc ggcccgtaga
    20101 ttaactgccc caacgcaaag cccagaatat aagtactcag ggtcatctgc gtactgcccg
    20161 ccggtacgcc aaactgcgct gaaattaccg gtagcgcggg cagatacata tcaatcgaca
    20221 gcggcatcaa catggccagc aggccaagga taaaaacaat agcaaacgac gaatgctgtc
    20281 gggtggtcac aacgggctcc tgaaagtcat tgaaaagtta gacgacgctg gcaatttctt
    20341 cttcagttaa cggacgatat tcaccggggg ctaaatcagc atccagcgta atgccgccaa
    20401 tacgttcacg atgcagctca accacatggt tacccacggc ggcgaacatg cgtttcacct
    20461 gatgataacg cccttcgctg atggtcagac gaacctgcgt tggggtaatc acttccagca
    20521 ccgccggctt agtgagatct ttttcgttat gcagctgcac gcctttagca aattgttctg
    20581 ccgtatcgtc agctacgggt gataccagcg tcaccagata ggtcttctcg caatgatggc
    20641 gcggagaagt aatgcggtgc gaccactgac catcatctgt cattagtacc agaccggtgg
    20701 tatcgatatc cagccgccct gcggcgtgca gtttccacgc taccggttca tctagaaaat
    20761 agagcaccgt tgggtgatca gggtcgtccg tggagcaaac ataaccctga ggcttattga
    20821 gcatgaagta acgtggaccg tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat
    20881 gttcaggaag cagtttgaac gctgcattac ggacgatttc gccatcgacg gtgacacgat
    20941 tgccgcggat ttcacgcccg gcaatagcac ggctaacgcc gagttgctgt gcgataaatt
    21001 tatcaagtcg catgtgtgtg attttgcctg taaaaaacgg aggtcgggta atgacccgaa
    21061 tatctgaact gttgtctgcc cagtatagcg gtctaataac atccctcaag ggaaaaagat
    21121 ccgtggcata ctattagcag aataatctac ctacgcgaga ccatgatttt tacacttcgc
    21181 ccatatcagc aagaagccgt ggatgccacg ctcaaccatt ttcgtcgtca taaaacccct
    21241 gccgttatcg tgctgcccac cggtgcaggt aaaagcctgg tgatagcgga actggcacgg
    21301 ctggctcgtg gtcgcgtact ggtgctggca cacgttaaag aactggtggc gcaaaaccat
    21361 gcaaagtatc aggcgctggg gctggaagcc gatatttttg ccgccgggct gaagcgtaaa
    21421 gagagccacg gtaaagtggt atttggcagt gtgcagtcgg tcgcccgtaa tattgatgcc
    21481 tttcagggtg aattttcgct gttgattgtc gatgaatgtc accgtattgg tgacgatgaa
    21541 gagagccagt atcagcaaat cctcactcac ctgacaaaag tgaatcccca cttacgcctg
    21601 ctggggctga ctgccacgcc ttttcgattg ggcaaaggtt ggatctacca gttccattat
    21661 cacggcatgg tacgcagcga tgagaaagcc cttttccgtg actgcattta tgagctgccc
    21721 ctgcgttata tgattaaaca cagctatctg acgccgccag aacgactgga tatgccagta
    21781 gtgcaatatg atttcagccg cttgcaggca cagagcaacg ggctgttcag cgaagccgat
    21841 ctcaaccgtg agctgaaaaa acagcaacgt attaccccgc acatcatcag ccagattatg
    21901 gagttcgccg aaaagcgtaa aggagtgatg attttcgccg cgacggtcga acacgcaaaa
    21961 gagattgtgg gattactgcc tgccgaagat gcagcactga ttactggcga cacccccggc
    22021 gctgagcgcg atgtgttaat tgaaaatttt aaagcccagc gttttcgcta tctggtcaac
    22081 gtcgcggtac tgaccaccgg atttgacgcc ccgcacgtcg atcttatcgc cattctgcgc
    22141 cctaccgaat cagtgagtct ttaccaacaa attgtcgggc gcggtctgcg tctcgctccg
    22201 ggcaagactg attgtttaat tcttgattat gcgggtaatc ctcacgatct ctacgcgccg
    22261 gaagttggta caccgaaagg caaaagtgac aacgttccgg tacaggtttt ctgccctgcc
    22321 tgcggttttg ccaacacctt ttgggggaaa acgaccgccg acgggacatt gattgaacac
    22381 tttggtcgcc gctgtcaggg atggtttgaa gatgacgacg gtcatcgcga acagtgtgac
    22441 ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgcag aaaatgatat tgccgctcgc
    22501 cgctgccgcg aatgtgacac ggtactggtt gatccggatg atatgttaaa agcggcgcta
    22561 cgactgaaag atgcgctggt attacgctgt agcggcatgt ctttgcagca tgggcacgac
    22621 gagaaaggcg aatggttgaa aatcacctat tacgatgaag acggtgcgga tgtgagtgag
    22681 cgtttccgtc tgcaaacacc tgcccagcgt accgccttcg agcagctttt tatccgcccg
    22741 catacgcgca caccgggcat cccgctgcgc tggatcaccg ccgccgatat cctcgcccag
    22801 caagccttat tgcgaccccc ggattttgtc gtcgcccgca tgaaaggcca gtactggcag
    22861 gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc gggcgcacga attacgcggt
    22921 taatggtagt tttgattgat gtataaggcg attgagtata gaatctcacc cgcttttgca
    22981 tacgcaaagc agatcactta cctgttgctg ggtcgcctgt agcaggatta atttaagaga
    23041 gaaagaaatg tttactatca acgcagaagt acgtaaagag cagggtaagg gtgcgagccg
    23101 ccgcctgcgt gccgctaaca agttcccggc aatcatctac ggtggcaaag aagcgccgct
    23161 ggctatcgag ctggatcacg acaaagtcat gaacatgcaa gctaaagctg aattctacag
    23221 cgaagttctg accatcgttg ttgacggtaa agaaatcaaa gttaaagctc aggacgtaca
    23281 gcgtcacccg tacaaaccga agctgcagca catcgacttc gttcgcgctt aattgctgaa
    23341 taagttgtaa aaaaccccgc tccggcgggg ttttttgtat ctgcagatta tgcctgatta
    23401 cggtattgct attttttgca ggccagataa ggcgttcacg ccgcatctgg cattcagaac
    23461 acaaaaccga ttaattgccg ccagacgtcc ggcgctgcaa ttggtcgcgc aaattcggcg
    23521 gcgtgccttt aatggtcagg gtatcggtcg ccggctccca gaagatgcgt tcacctaaca
    23581 gcatcgcatc aaagttaata gtcaagccac cgccgctacc ggcaaacttc gtcaactgac
    23641 gcagtgtgct ccggtctgcc gggaagctct cttccagttc gtagcctttt tcagccgcaa
    23701 actccgtgaa gctcacttcg cttacgcctg ccagctcttt cgacagcgat tccagttcga
    23761 tctcttcgcc tgcctgcagc tgctcattac agtagctata aacctgctgg cgcacgttct
    23821 gtcgctctgc tttatccagt tgtgcttcgg cagtgaaatc atcaaccgct tgcaacagcc
    23881 cacggttttg cgctttcgtg ttcaatcctt cgctggcccc gaggaaatcc ataaagaaat
    23941 ccgccacttt gcgccctact cgccctttca ggaaagtgag atagcgggtg gactctggat
    24001 tggtttccca ttcggttaaa tcgatacgcg caacaatatc cgcatgattg atatcaagat
    24061 aatgggttgg gttgatgtcg agattttcgt taacacgcat actgctcagg ttgctcagaa
    24121 ctgccaccag cagatactcc accgccagat aacggtagtg gcaaaacagc acgaatccac
    24181 cgtcagcaaa aggatatttc gctaattcgt cacgcaggcg accagttgcc gcgcggctaa
    24241 atgccagaaa gtcctcttcg ccctgacgct gtaagcgcag cgtctgcgcc agttcactct
    24301 cttcgctaaa caagccgtaa gctttatttt tggcgctata gacccgatgc agttctgcca
    24361 ccatctcaac gacggtttct gtcggttcca gcaatgaatc gcgcaacacc agctcaaggt
    24421 tctgctcatc acgcttgata agctggtgca gagcaatctg gttgatatcc agactcatga
    24481 taaactctcc tttaagaccg ggcggtattc aaccaccgcc ggttaagcga cgcaataaaa
    24541 gatggcggtc attttacaga aggcagcgta acgagaatga tttaacgcat aaaaaagcag
    24601 aaaaaaaacc gttgctacgg taatatgttg ccctttcatc aactaactga tttcgattta
    24661 tgccacaaat ttcccgctac agtgatgaac aggttgaaca attgctcgca gagctgctca
    24721 acgtactgga aaaacataag gctccgaccg acctttccct gatggtgtta ggcaacatgg
    24781 tcactaacct tatcaacacc agcattgccc cggcccaacg ccaggcaatt gccaactctt
    24841 ttgcccgcgc cttacagtcc tctatcaacg aagacaaagc gcactaaggg aaacagataa
    24901 caggttatgg taactcatcg tcagcgctac cgtgaaaaag tctcccagat ggtcagttgg
    24961 gggcactggt ttgcactgtt caatattctg ctttcgctcg tcattggcag ccgttacctg
    25021 tttatcgccg actggccgac aacgcttgct ggtcgcattt attcctacgt aagcattatc
    25081 ggccatttca gcttcctggt gttcgccacc tacttgctga tcctcttccc gctgaccttt
    25141 atcgtcggct cccagaggct gatgaggttt ttgtccgtca ttctggcaac ggcgggaatg
    25201 acgctattac tgatcgatag cgaagtcttt actcgtttcc atctccatct taatcccatc
    25261 gtctggcagt tggttatcaa cccagacgaa aatgagatgg cgcgcgactg gcagctgatg
    25321 ttcatcagcg tgccggttat tttattgctt gaactggtgt ttgcgacgtg gagctggcaa
    25381 aagctgcgca gcctgacgcg tcgtcgacgc ttcgcgcgcc cgctggccgc attcttattt
    25441 atcgccttta tcgcctcgca tgtggtgtat atctgggccg atgccaactt ctatcgcccg
    25501 atcaccatgc agcgcgctaa cctgccgctt tcgtacccga tgacggcgcg acgttttctt
    25561 gagaagcatg gtctgcttga tgcgcaggag tatcaacgcc gtcttattga gcaaggtaat
    25621 ccagacgccg tttccgttca gtatccgtta agcgaactgc gctatcgcga tatgggcacc
    25681 gggcagaatg tgctgttgat tactgtcgat ggcctgaact actcacgctt cgagaagcag
    25741 atgcctgcgc tggcaggttt tgctgagcaa aatatttcgt tcacgcgcca tatgagctcc
    25801 ggcaacacta cagacaacgg catctttggc ctgttctatg gtatctcgcc gagctatatg
    25861 gacggcattc tgtcgacccg tacgcctgcg gcattaatta ctgcgcttaa tcagcaaggc
    25921 tatcagctgg ggttattctc atcagatggc tttaccagcc cgctgtatcg ccaggcattg
    25981 ttgtcagatt tctcgatgcc gagcgtacgc acccaatccg acgagcagac cgccacgcag
    26041 tggatcaact ggctgggcca ctacgcacaa gaagataacc gctggttctc gtgggtttct
    26101 ttcaatggca ctaacattga cgacagcaat cagcaggcat ttgcacggaa atatagccgg
    26161 gcggcaggca atgtcgatga ccagatcaac cgcgtgctca atgcactgcg tgattctggc
    26221 aaactggaca atacggttgt gattatcact gccggtcggg gtattccgct gagcgaagac
    26281 gaagaaacct ttgactggtc ccacggtcat ctgcaggtgc cattagtgat tcactggcca
    26341 ggcacgccgg cgcagcgtat taatgcgctg actgatcata ccgatctgat gacgacgctg
    26401 atgcaacgcc tgctacatgt cagcacacct gccagcgaat attcgcaagg tcaggatttg
    26461 ttcaaccctc aacgccgtca ttactgggtt actgcagcgg ataacgatac gctggcaatt
    26521 accaccccga aaaagacgct ggtgctgaac aataacggta aataccgcac ttacaactta
    26581 cgtggtgaaa gagtgaaaga tgaaaaacca cagttaagtt tgttattgca agtgctgaca
    26641 gacgagaagc gttttatcgc taactgatta attataaatc agttagcgaa atatcttact
    26701 tgcaatcggt gtggaaaacg gtagtattag cagccacgag tcggcacgta gcgcagcctg
    26761 gtagcgcacc gtcatggggt gtcgggggtc ggaggttcaa atcctctcgt gccgaccaaa
    26821 aatcccaaga aaaaaccaac ctttacggtt ggttttttta tatctgcaat taatttgata
    26881 aacagaccgt gacacatcac agcctgttta ttttctgtta tcagaacgtc cagaccactc
    26941 ccgcctgagt attccacggc gattctaccc ctgcgccatt gccatagctg actgacaaat
    27001 gaccgcttaa cgtcggggta aacgatgacc ttatcccagc ctgataaacg ccacgggttc
    27061 cagccacatc attattaaat ttgccatcgt cattaacttt cacatggtta gaatcggcct
    27121 attcctgacg cacggcggct ttcagccagg gttccaacgt cgtaccgttt tgcaggtcca
    27181 tgtgatagct taccgccgtt cccgcttcag cgcgtaatat ccgggtattt cccacatccg
    27241 cgcgcatgcc gtttgataac gtgtagtcct gaccatctgt ggtaaagccg gtaaaggcga
    27301 gataggggct aacactccac aatccgtcaa cccaacggaa cccgctctca acatgagcac
    27361 ccgcgccgtt actattgtaa tcgccaaacg ctgttgcccc attactcatc ttgccatgga
    27421 tggtgttggc aaaacggtca actttcacca ccccatcaac ataggcaccg ttctgatgct
    27481 cccaaccggc ataagccccc agggtgtagc tatcaatatt gcctttgccg ccgcgatcaa
    27541 aaccaatatc agaatgagag taatcaaaga acaagccgcg aattgtgctg ctttcttcac
    27601 gggagaaacg gctatcgata ccgagcgtca ggcccgtcaa tgtttgctca aagccagctc
    27661 ccgcatcagt ggtcacgttg ttgcgggtgt taattgccga actccacatc gccgtatcgt
    27721 aattaacgcc ttttacgcta ccaagacgct cacgcacggt gtccagttct gcatcaaata
    27781 ccagcggttg tgctgctgcc atgttcagca catcagtggt tgaaggggta atttgcgcgc
    27841 gattctctgc gaggctccag ctatggttgc cattatccag caaggtatat tcatacgtac
    27901 cgatatcaac aacgcctccg gcattgccca acgtgaatgc agcatcaccg ccgcccgtcg
    27961 ttaccagtgt aaggctatct cctgctgccg ggctggcccc cgtgtccgtc acgaatattt
    28021 tgaaatcacc tgttgcctga ccgatgacgt tgagctgatc gctctgatga ttagccatat
    28081 cggtacgcat ataaaattgc cgtttcccga cagggtattg gtggtcaacg taatgaaatt
    28141 ttccgcactt gccgtcacag atgaacgagt cactggctca gccatcataa tcacatttgc
    28201 gccggacatc gcaaggttgg ctaagttgag gcgagtgttt tggttcgtcg tagtgatatc
    28261 ggcaatgatg gtccatgcgc tattttcgag cgaaacgtcc gcttggctgg tatccacggc
    28321 accatgcgtt ctaaaagaag cgccctcaga aaccactacc ttacccacca acatagcggg
    28381 tgcataattt attttcggct tcatgacctc aagaatgcca tcattccctc tgactgacac
    28441 gttaaccatt gtgccagccc agacattagc gcggccattg ttgataacca tattttcaga
    28501 gatggcttta gaggaatacg tgatacttcc attctgatct gatcgaccga gatcatatac
    28561 agcatttgcc tgcactttcg taccagtatc ctttcccagc gattgcatag tggcatgctc
    28621 atcaaggata gtgtcaatgg cctgagtgtc ctccataaca atgagcccgg agccatcatc
    28681 cagttcataa ttttttgaca caccatcctt tatactaaat tggcctttac tgtttgtacc
    28741 actcacttcc agcgcattcg ttgagacaat taatttcccg cctgcttttt tatcaacgcc
    28801 agttgcagtc ccgccatcca taacctccag taagccgcca ctatccacag tggtattata
    28861 agcgaagtca ttttcttcaa ctcgcaaact tccgccgttt tccaacagca tattgttagc
    28921 aataccattc ttgatttcga actgaccgag acggtttgtt ccgaatacct ccatgacccg
    28981 cgtggttgtt ttaatagcac cgcctgcttt ctgatctacc gcaaatgcta atcccccgtc
    29041 tttaacctcc agtaatcctc ccttttcgat agcggcaaag acggcaatcc catctttttc
    29101 aacaattaag cgcccgcctt taatcgtggg atcattcgcc gcg
//
LOCUS       DAZRUF010000021        28898 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZRUF010000021 DAZRUF010000000
VERSION     DAZRUF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28898
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.021"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(186..1097)
                     /gene="gsiD"
                     /locus_tag="ACHXOL_000787"
     CDS             complement(186..1097)
                     /gene="gsiD"
                     /locus_tag="ACHXOL_000787"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR6807842.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1100..2020)
                     /gene="gsiC"
                     /locus_tag="ACHXOL_000788"
     CDS             complement(1100..2020)
                     /gene="gsiC"
                     /locus_tag="ACHXOL_000788"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR6807843.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2038..3576)
                     /gene="gsiB"
                     /locus_tag="ACHXOL_000789"
     CDS             complement(2038..3576)
                     /gene="gsiB"
                     /locus_tag="ACHXOL_000789"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR6807844.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3596..5467)
                     /gene="gsiA"
                     /locus_tag="ACHXOL_000790"
     CDS             complement(3596..5467)
                     /gene="gsiA"
                     /locus_tag="ACHXOL_000790"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR6807845.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5454..6419)
                     /gene="iaaA"
                     /locus_tag="ACHXOL_000791"
     CDS             complement(5454..6419)
                     /gene="iaaA"
                     /locus_tag="ACHXOL_000791"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR6807846.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6623..7858
                     /gene="moeA"
                     /locus_tag="ACHXOL_000792"
     CDS             6623..7858
                     /gene="moeA"
                     /locus_tag="ACHXOL_000792"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR6807847.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7858..8607
                     /gene="moeB"
                     /locus_tag="ACHXOL_000793"
     CDS             7858..8607
                     /gene="moeB"
                     /locus_tag="ACHXOL_000793"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR6807848.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8786..9448)
                     /gene="fsa"
                     /locus_tag="ACHXOL_000794"
     CDS             complement(8786..9448)
                     /gene="fsa"
                     /locus_tag="ACHXOL_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR6807849.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9579..10478
                     /locus_tag="ACHXOL_000795"
     CDS             9579..10478
                     /locus_tag="ACHXOL_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR6807850.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10484..12916
                     /locus_tag="ACHXOL_000796"
     CDS             10484..12916
                     /locus_tag="ACHXOL_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR6807851.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13062..13877
                     /gene="ybiV"
                     /locus_tag="ACHXOL_000797"
     CDS             13062..13877
                     /gene="ybiV"
                     /locus_tag="ACHXOL_000797"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR6807852.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALNGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14029..15294
                     /locus_tag="ACHXOL_000798"
     CDS             14029..15294
                     /locus_tag="ACHXOL_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR6807853.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15261..16421)
                     /locus_tag="ACHXOL_000799"
     CDS             complement(15261..16421)
                     /locus_tag="ACHXOL_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR6807854.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQCAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            16659..17960
                     /locus_tag="ACHXOL_000800"
     CDS             16659..17960
                     /locus_tag="ACHXOL_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6807855.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            17975..20338
                     /locus_tag="ACHXOL_000801"
     CDS             17975..20338
                     /locus_tag="ACHXOL_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR6807856.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(20518..22113)
                     /locus_tag="ACHXOL_000802"
     CDS             complement(20518..22113)
                     /locus_tag="ACHXOL_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR6807857.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22332..23252
                     /gene="ldtB"
                     /locus_tag="ACHXOL_000803"
     CDS             22332..23252
                     /gene="ldtB"
                     /locus_tag="ACHXOL_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR6807858.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23311..24429)
                     /locus_tag="ACHXOL_000804"
     CDS             complement(23311..24429)
                     /locus_tag="ACHXOL_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR6807859.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24426..24893)
                     /gene="mntR"
                     /locus_tag="ACHXOL_000805"
     CDS             complement(24426..24893)
                     /gene="mntR"
                     /locus_tag="ACHXOL_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR6807860.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25079..25207
                     /gene="mntS"
                     /locus_tag="ACHXOL_000806"
     CDS             25079..25207
                     /gene="mntS"
                     /locus_tag="ACHXOL_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR6807861.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            25479..27062
                     /gene="opgE"
                     /locus_tag="ACHXOL_000807"
     CDS             25479..27062
                     /gene="opgE"
                     /locus_tag="ACHXOL_000807"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR6807862.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27111..27626)
                     /gene="ompX"
                     /locus_tag="ACHXOL_000808"
     CDS             complement(27111..27626)
                     /gene="ompX"
                     /locus_tag="ACHXOL_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR6807863.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(27679..27744)
                     /locus_tag="ACHXOL_000809"
     CDS             complement(27679..27744)
                     /locus_tag="ACHXOL_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6807864.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            27979..28866
                     /gene="rhtA"
                     /locus_tag="ACHXOL_000810"
     CDS             27979..28866
                     /gene="rhtA"
                     /locus_tag="ACHXOL_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR6807865.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         6966 a         7949 c         7286 g         6697 t
ORIGIN      
        1 ctcagcatgt taatacattt aacaaaataa ctatctgaac gctgtgccgc tgcaaaaata
       61 gtcccttgct gcggcttttt aaccaaatta cccgattcta caaaatcagc agaaagttac
      121 aacacttgct tagggcaata atttcctgtg aggtgattac cctttcaagc aatattcaaa
      181 cgtaactatc ctttaatttt cggatccagc gcatcgcgta aaccatcgcc caacaaattg
      241 aacgccagta cggtcagaaa aatagccagg gccggaaaaa cagcgacatg cggcgcgata
      301 accatatccg ctcgtgcctc attgagcatt gctccccact ctggtgtcgg cggctgcgca
      361 ccgaggccga gaaatgacag gctggcggca gagataatcg aggtgccaat gcgcatggtg
      421 aaaaacacca cgatagacga gacggtccca ggcaagatat gacgcaataa aatggtcata
      481 tcgctggcac caatactgcg tgctgactca ataaaggttt gctgtttcaa caccagcgtg
      541 ttgccgcgca ccagtcgggc aaacgcgggg atggaaaaaa tggcgactgc gataatcacg
      601 ttagcaatgc cgcttcccaa caccgcaaca acagcgatcg ccagtaaaat acccgggaag
      661 gcaaacagca catcgcaaat gcgcatgatc agccgatccc accagccttc ataatatcca
      721 gccaacaagc ccagcaacgt cccgatcgcc gcaccgataa acacggcaaa cacgcccgcc
      781 gccagcgaga tttgcgcacc aaccaggaca cggctgaaaa tgtcgcgccc cagtgaatcg
      841 acgccaaacc agtgctgcaa agaaggtccg ttattcagat tgtcataatc aaaataattt
      901 tcggcgtcat agggagcgat ccagcgtgca aaaatggcca ccacaatcaa taaaataacg
      961 aataatgcgg cggtcatcgc catatgctga cggcgaaatc gtcgccagaa ttcatgccac
     1021 ggtgtacgca cctggtcagg tttgaccagt ggcatggcgt ttaacaccgc ctgacgtcgc
     1081 cagttaaata gtcgcatcct tacttgtacc tgatagccgg gttaatggcg gcgtaaagca
     1141 catccaccac taagttgata agaataaatt ccagcgagaa aagcagaatt tccgcctgaa
     1201 tcaccgggta atcgcgcatt tctacggagt caacgagtaa gcgtccaagt ccgggccagt
     1261 tgaaaacttt ctccaccacg atagaaccac cgagcaaaaa gccaaactgt aagcccatca
     1321 tggtcactac cgggatcatc gcgttacgta gcccgtgttt gaggacaacc caggtttcgc
     1381 tcaccccttt cgccctcgcg gtacgcatat aatcttcgct taaaacatcg acaaacgacg
     1441 cgcgggtaaa gcgcgccatc acggctgcca ctgccgcgcc gagcgtcagg gagggtaaaa
     1501 tgtagtgctg ccaactgtct gctcccacgg taggcagcca gcccagttca acggagaata
     1561 cctgaattaa aagcatcccc agcgcaaatg ccggaaacga gatccccgac accgcaatgg
     1621 tcatactcaa tcgatccggc caacgattac gccagacggc ggcgataatt cccgccgcca
     1681 taccaaatat caccgcccag accatactgg ttatggtcag ccacagcgtt ggcataaagc
     1741 ggctggcaat ctcatcggca accggacgac gcgacaccat cgacaggcca aaatccccct
     1801 gcacagcatt gctgatatag tgccagaact ggtgatacag cggctgatcc aaccccagct
     1861 gctgacgcac cagttctata acctgcgcat cagcttcgag cccggcaatc aatcgcgccg
     1921 gatcgccggg cagcatatgc acaaataaaa acaccagcac cgagacgata aacagcgtcg
     1981 gaatcaaccc cagtaagcgt ttgataacgt aattaagcat tccactccct gcgttgctta
     2041 ttgcaaatcc gcgtcttcaa agctgaagcc ggtgtctggc atgatccaaa aaccggtcag
     2101 gtttttactg tgtgccgaca ccagtttttc taccaccagc gggatccacg gcgactcttg
     2161 ccagatgata tcctgcgccg ccttatacaa gcgggtcttt tccgccggat cattagtttt
     2221 cagtgcctga gccaggaagt cgtccacctg tttattgctg taaaatgcgg tattaaacag
     2281 cgtcggtggc cagttttgcg aggcgaacag tggcgatagc gcccagtccg cttcgccggt
     2341 tgaagccgac cagccagtgt agaacatccg cacgccgctc tctttttgcc ctttaccttc
     2401 aacttctgcc gcccgctgtc cggcatccat cgcggtcacc tgggctttaa tcccgacctg
     2461 cgctaactgc tgctgggtaa attgcagcac tttctgcgcg gtgctgtggt tatgtgacga
     2521 ccacagcgtg gtactgaaac cgttgggata tcccgcctct ttcagtaatt cgcgcgcttt
     2581 cactggatcg taaggccacg gtttataact ttgcgcgtag gcgatacttg gcggtaccac
     2641 accagtagct ggcgttgcat agcccgcaaa ggcaactttc accagcgccg gacggttaat
     2701 ggcgtaattc agcgcctcac ggaccttcgg gttatcgaac ggcttttgcg tcacgttcat
     2761 actgatataa cgctgcataa ttgacggact ggccatcaac tcgatatttt tgtttttctc
     2821 cagcagtgtg gcctgctcgt agggaatggg gaaagcaaac tgcgcttcac cggtttgcag
     2881 cattgccgcg cgggtgttgt tatccgccac cggacgccag gttatgctgt ccagtttggg
     2941 caatcctggc tgccagtaac ccgcgaattt tttcaccttc acaaaatcgg tctgattcca
     3001 ggtatccagt tcatacggtc cggttcccac cggatgaaaa ccaatctcct tgccatattt
     3061 ttccagcgct gccggtgaaa tcatcgcggt cgccggatgg gcaagaatat taataaacgc
     3121 tgagaacggc tgtttgaggg taatctttac cgttgtcgga tcgatcgcct ccgttttggc
     3181 aatattctta tacaggttat agcgtttaag atgattcgcc ggatcgctgg cccggtccag
     3241 attcgctttt accgccactg cgttgaaatc ggtgccatcc tggaatttaa tgccttcccg
     3301 caatttcacg gtgtaagtaa tgccgtcatc ggaaacggta taactctccg ccagcacgtt
     3361 tttcagtttc atctctttat ccagaccgaa cagcccctgg taaaacgatt tcgctacggc
     3421 ctgagataac gtgtcattcg cgtcatacgg atcgagcgtg gtgaaattcg atcctaccgc
     3481 caccaccaca tctttggcag cgaatgcaca agatgccatc aacgctgtcg caatgcccag
     3541 cgccactaac ccactacggt gtacagctct tgccatttta ttctccgcct gaatgttatc
     3601 tacgcatgaa tgcgtattct gattgtggtt gtgcgacgta atgccccggc ccgacgcatt
     3661 gcaacgagac ggctgccacc tcttcgccac gcagatgaat attgctggga agatcgtccg
     3721 acagcagtac acgctgcggt cgttgtcggg acggttcagc gaccggaact gccgccagta
     3781 atttacgcgt ataaggatgc tgcgggtttt cgaagaccgc gcgccgtgga ccaatttcaa
     3841 caatttgccc gagatacatc accgccacac gatgactaat ccgctctacc acggccatat
     3901 cgtgggagat aaacagatac gcaatgccga aatcacgctg gagatcgagc aacaagttga
     3961 taatctgccc gcgaatagaa acatccagcg ccgaaacggc ttcgtcggca atgatcactt
     4021 ttggattcaa tgccaacgcg cgagcaatgc agatgcgctg gcgctgaccg ccggaaaact
     4081 catgcgggta acgccaggca tgttcaggta acaggcccac gcgctccagc aaccacgcaa
     4141 cgcgtgcagc cgcgtcttta cctggcaata aaccgtgtac acgcagcggt tcgataatcg
     4201 aatcaccgat ggtctgacgt gggtccagcg aagcgtaagg gtcctgaaaa ataaactgaa
     4261 tatcccggcg caatgcctga agtttgccgg gtgacaaggt atcgattctc tgaccgttaa
     4321 agataatttc gccgccctgc gattcgacca ggcgcagcaa cgcccgcccg gtagtggatt
     4381 taccgctgcc agactcgccc accagcgata gcgtttcgcc aggccagaga tcaaaactga
     4441 ctttctcaac ggcatgcact tcccgcgtta cgcgattcaa caaaccgctg cgcaaaggga
     4501 aacgggtgac aagattacgc actcgtaaaa caggttcgcc atccaccacc gttttctgct
     4561 cgatgggggg ggcctgtttc gctggatgtt caagcgatat caacgggaaa cgtcggggat
     4621 aatccaaccc tttcatcgca ccaagttgcg gaacagcagc taacagcgca cgggtgtaag
     4681 gatgttgcgg tgcatgaaaa atctgttcga cggtacccgt ttccaccgcc tcgccctgat
     4741 acatcaccag tacccgatcg gcaatctctg ccaccacgcc catatcgtga gtgataaaga
     4801 taacgcccat cgacatctct ttttgcaata ctttgattaa ttgcaggatc tgcgcctgaa
     4861 tagtgacatc cagcgcggtg gttggctcat cggcaatcag caccgccggg cggcatgaca
     4921 gcgccatcgc aatcatcact cgctggcgca tcccgccaga gagttgatgc ggataacgtg
     4981 aaagaatggt ttgtgcctca ggaatgcgta cctgatccag catccgcttc gcctcgacca
     5041 tcgcttcttc acgactggcg ttctgatgca gacgaattga ttcggcaatc tgttcaccca
     5101 cagtaaatac cgggttcagc gatgtcatcg gctcctgaaa tatcatcgcc atatccgcac
     5161 cgcgaacatg gcgcatttgt gcagcgctct gctcgctaag ttcaatcact tcgcgactgc
     5221 gccgctgcaa cagcatttta tcgcactgta ctaaaccgcc cgcctgttcc aacaggcgca
     5281 tcaatgccaa cgcagtcact gacttaccgg agccggattc gccaacaatt gccagcgtct
     5341 caccgcgttg cagactaaaa gagagattgc ggaccgcagc tattttctgc tggtcctgca
     5401 taaaggcaat attcagattt tcaaccgcca gcacattacc ggcatcaagt tcatcactgt
     5461 gtggcaacgg tgtccccttt ttcacggtag ataccggtgg ttggcgtatc gcctgcgtag
     5521 ccccaggcgc gatacattcc ttcggtgtta aacggtagcg cgacattccc ttcatggtcg
     5581 atagcgatta agccaccgct accgccaagc gcagggagtt tttccattac tacccgctcg
     5641 caggcttccg cgagacttaa tccgccgtaa tccattaacg cggcgatgtc atatgccgcc
     5701 agcgcgcgga tgaagacttc gcccgtgccg atacaagaaa ccgccacact ggcgttattg
     5761 gcgtagcatc cggcacccac taagggacta tcgccaactc gtccgggtaa tttattggtc
     5821 attccgcctg tggacgtggc tgccgccaaa ttgccgtcta aatccaacgc cacggccccc
     5881 acggtgccca ttttctgttt ttcatccagt ggcgcaccgc tatggtcgag gacggttgcc
     5941 ccttccttgc gcgctgccag tagttgttca taacgcaaag gcgtggagaa aatctccggc
     6001 gagacgcgct ccatgccacg agcaaacgca aaattttctg ccccttcgcc aatcatcatc
     6061 acatgcgggc tttgctccat caccagccgg gcggcaagaa ccggattacg cagatgacta
     6121 acgcccgcca ccgcaccggc tttcagggtg ttaccatcca tcacacaggc gtccagttca
     6181 tgggtttcat cacgcgtaaa gacagcgcca attccggcgt taaacagtgg acactcttcc
     6241 agcagacgca ccgcttccgt caccacatcc agcgcacttt cgcccgcttc cagcattttc
     6301 tgcccggttt caacaatggc agacaacgcc tcaatgtagc gtaattcctg ttgcagactc
     6361 atctgcgcgc ggctaattgc acctgcgcca ccatgaattg caatgactgc tttgcccata
     6421 gtattcgtcc attaagggtg gctcgccact tttgctataa atatcagaat ttttatgaat
     6481 tactcattca atttttgaat atagaaagat ggatatggca tgtaaaggca ggagccaacg
     6541 ctacgcccga ggtggcatac agtctgtggc gtttttctga cataataggc aaattcgatt
     6601 ttgcctccgc aggagtgttt tcatggaatt taccaccgga ttgatgtcgc tcgacaccgc
     6661 gcttaatgag atgctttctc gcgtcacccc actgaccgcc caggaaacgc tgccactggt
     6721 acagtgtttt ggtcgtattc tggcgagcga tgtcgtttcg ccgcttgatg ttccggggtt
     6781 tgataactcc gcaatggacg gctacgcggt gcgtttagcc gatattgcct ccgggcaacc
     6841 gctgcccgtt gccggtaaat cctttgccgg tcagccgtat catggtgaat ggcctgcggg
     6901 cacctgcatt cgtattatga ccggtgcgcc ggtgccggaa ggctgcgaag cggtggtgat
     6961 gcaggagcag actgaacaaa cggacaatgg cgtgcgtttt actgctgaag tgcgtagcgg
     7021 gcaaaatatt cgccgtcgcg gtgaagatat ctctgcaggt gcggttgttt tcccggcggg
     7081 aactcgcctg actaccgcag agctgccagt aattgcttca ctggggattg ccgaagttcc
     7141 ggtgattcgt aaagtgcgtg tagcgctttt ttctaccggt gatgaactcc agttgcccgg
     7201 tcagccgctg ggcgacggcc aaatctacga caccaaccgt ctcgccgtac acctgatgtt
     7261 agaacagctg ggttgcgagg taatcaactt agggattatc cgcgacgatc cccatgccct
     7321 gcgcgccgca tttattgaag ccgacagcca ggcggatgta gtgatcagtt ccggcggtgt
     7381 ttcagtgggt gaagcggatt acaccaaaac gattcttgaa gagctggggg agatcgcctt
     7441 ctggaagctg gcgattaaac caggtaaacc gttcgcgttc ggtaaactca gcaatagctg
     7501 gttctgcggc ctgccgggca acccggtttc agcgacgctg accttctatc aactggtaca
     7561 gcctttgctg gcaaaactaa gcggcaacac cgccagcggc ctgcccgcgc gccagcgcgt
     7621 acgcacagcg tcccgcctga aaaaaacgcc aggacgtctt gatttccagc gcggcgtgct
     7681 gcaacgcaac gccgacggtg aacttgaagt gacgaccacc ggacatcagg gttcacatat
     7741 atttagctcc tttagtctcg gcaactgctt tatcgtgctg gaacgcgatc gcggcaatgt
     7801 ggacgtgggc gaatgggtgg aagtagaacc gtttaacgcg ttgttcggag gcctgtaatg
     7861 gcggaactca gcgatcagga gatgctgcgc tacaaccggc aaatcattct gcgcggtttt
     7921 gattttgacg gtcaggaggc gctaaaagat tctcgtgtgc tgatcgtcgg cctcggtgga
     7981 ctcggctgcg cagcttcgct gtatctggca agcgccggtg tcggcaacct gacgctgctc
     8041 gacttcgaca cggtttcgct ctcgaatctg caacgccaga cactgcacag tgatgccacg
     8101 gtcgggcaac cgaaggtgga atccgcccgt gacgccctga cgcggatcaa cccacatatc
     8161 gcgattacgc cagtcaatgc actgctagat gacgcagaac ttgcagcatt gattgctgaa
     8221 cacgatctgg tgctcgactg tacggataac gttgcggtac gtaatcaact gaacgcaggc
     8281 tgttttgccg cgaaggtgcc gctggtttcc ggcgcggcaa ttcgtatgga agggcaaatc
     8341 acggtcttca cttatcagga cggcgaaccg tgctatcgct gccttagccg tttgtttggc
     8401 gagaacgcat taacctgcgt agaagcaggc gtgatggcac cgttgatcgg cgtaattggt
     8461 tcgttgcagg caatggaagc gatcaaactg ctggcaggtt atggcaaacc gaccagcggg
     8521 aaaatcgtca tgtacgatgc gatgacctgt cagtttcgcg aaatgaaact gatgcgcaat
     8581 ccggggtgtg aggtgtgcgg gcagtaattg ccacctccag gcctgataag acgcgacaag
     8641 cgtcgcatca ggcatttgct ccagacgcca gatgcggcgt gaacgcctta tctggcctac
     8701 ggttcgggca cagatttcca agcgcggcag gcatcggtgc actccgccac gtcatcagca
     8761 atttatgagg atgcagaacg tccggttaaa tcgacgttct gccaaacgct ccctgccagt
     8821 cctgctcaaa cttcgccaca gcggcttcca ccgccggata gctaatcatc tgttgtgcca
     8881 catccagtgg cagagtaatt gattcacatc ctgccagtaa gcagtccagc gcctgacgcg
     8941 gggttttgaa actcgctgcc agcactttcg cctgcggcgc atgcattttc aataactggt
     9001 gtaagtcggt cacagtctga atgccgctac cgccctgagc atcaatacga ttaacgtaag
     9061 gcgcaacata ttccgcacct gccagcgccg acagcaaccc ttgtgctgcg ccatataccg
     9121 cggttcccag cgttggaatc ccttccgctt ttaacatctt aatagctgcc agcccctcgg
     9181 cggtcaccgg aactttcacc acgatatccg caataataga acgcaattta cgtgcgtcat
     9241 tcaccatccc ttcagcagtg gtagccatta cctgggcaaa cagacgcccc tgaccgccca
     9301 tcgcttcatg aagttgcgga agcacaacat ccagcggttt tttacccgcg gcgataatgc
     9361 ttgggttagt ggtcacaccc gccagcggaa aaatacgtga cagcgccttc accgcaacaa
     9421 cgtctgaagt atccagatac agttccataa ccatcctcaa aatgtacttt ttgtctgaat
     9481 gtagactatc acgggaaaac tctctgaaca gttgacgcac atcaatctaa ctttcattcg
     9541 aaagtatttt aatgtttgaa cgaaacagag aggcaactat gattttcaat attcagcgct
     9601 actcgaccca tgatggcccc ggtatccgca cggtcgtatt tcttaaaggc tgttcgctgg
     9661 gctgccgctg gtgtcagaac ccggaaagcc gcgcccgcac gcaggatctg ctgtatgacg
     9721 cacgactgtg tctggaaggc tgcgagctgt gcgctaaggc cgcgccggaa gtgattgagc
     9781 gcgcgctgaa tggtttgctt attcatcggg aaaagttaac cccggagcat ctgacggcgt
     9841 taaccgactg ctgtccgaca caggcattaa ccgtgtgtgg tgaagtgaaa agcgttgagg
     9901 agatcatgac gaccgttctg cgcgataaac cgttttacga tcgcagcggc ggcggtttaa
     9961 cgctttcggg tggtgagccc tttatgcagc cggaaatggc gatggcgcta ctgcaagcca
    10021 gccacgaggc aggcattcat actgcggtag aaacctgtct gcatgtgccg tggaaatata
    10081 tcgccccttc tctgccctat atcgatctgt ttcttgccga tttaaaacac gttgccgacg
    10141 cgccgtttaa acagtggacc gacggtaacg ccgccagagt gctggataac ctgaaaaaac
    10201 tcgccgcagc gggcaaaaaa atcattatcc gcgtgccgct gattcagggc tttaatgccg
    10261 acgaaacctc tgtaaaagct attaccgatt ttgccgccga cgagctgcac gttggcgaaa
    10321 ttcatttcct gccctaccac acgctaggca tcaacaaata tcacttactt aatctgccct
    10381 atgacgcccc ggaaaaaccg cttgatgcgc cagaactgct cgactttgcc cagcagtatg
    10441 cctgccagaa agggttaacc gcgaccttac gaggataaca accatgacca cactgaaact
    10501 ggacacgctc agcgaccgca ttaaagcgca caaaaatgcg ctggtgcata ttgtgaaacc
    10561 gccagtctgt accgagcgcg cgcagcacta taccgagatg tatcaacaac atctcgataa
    10621 gccgatcccg gtacgtcgcg cgctggcact ggcgcatcac ctggcgaatc gcaccatctg
    10681 gatcaaacac gatgagttga tcattggcaa ccaggcaagc gaagttcgcg ccgcgccgat
    10741 cttcccggaa tatactgtct cgtggatcga aaaagagatt gatgatctgg cagatcgtcc
    10801 cggtgctggc tttgcggtga gcgaagagaa caaacgcgtt ctgcatgaag tgtgcccgtg
    10861 gtggcgcggt cagaccgtac aggatcgctg ctacggcatg tttaccgatg agcaaaaagg
    10921 tctgctggcg accggaatca ttaaagcgga aggcaatatg acctccggcg atgcgcacct
    10981 ggcggtgaat ttcccgctgc tgctggaaaa aggtcttgat ggtctgcgcg agaaagtagc
    11041 ggaacgtcgc tcgcgcatca atctgacggt actggaagat ttgcacggcg aacagttcct
    11101 gaaagcgatt gatatcgtgc tggtggcagt cagcgaacac attgaacgtt ttgcagccct
    11161 ggcgcgtgaa atggccgcga ccgaaacccg cgaaagccgt cgcgatgaac tgctggcgat
    11221 agcagaaaac tgcgatctta tcgcccacca gccgccgcag actttctggc aggcgctgca
    11281 actgtgttac ttcatccaat tgatattgca gatcgaatct aacggtcact cggtatcgtt
    11341 tggtcgtatg gaccagtatc tctacccgta ctatcgccgc gacgttgaac tcaaccagac
    11401 actggatcgc gaacacgcca tcgaattgct gcatagctgc tggctgaaac tgctggaagt
    11461 gaacaagatc cgctccggct cacactcaaa agcctctgcg ggaagtcctc tgtatcagaa
    11521 cgtcaccatt ggcggacaaa ctctggttga tggtcaacca atggacgcgg tgaatccact
    11581 ctcttacgct atcctcgaat cctgcggtcg cctgcgttcc actcagccta acctcagcgt
    11641 gcgttaccat gcaggaatga gtaatgattt cctcgacgcc tgcgtacagg tgatccgttg
    11701 cggcttcggg atgccggcgt tcaacaacga cgaaatcgtg atcccggaat ttattaaact
    11761 cggtattgaa ccgcaggacg cttacgacta cgcagcgatt ggttgtatcg aaaccgccgt
    11821 cggtggcaaa tggggctatc gctgtaccgg catgagcttt atcaacttcg cccgcgtgat
    11881 gctggcggcg ctggaaggcg ggcgtgatgc caccagcggc aaagtgttcc tgccacaaga
    11941 aaaagcgttg tcggcaggca actttaacaa tttcgatgaa gtaatggacg cgtgggatac
    12001 gcaaatccgt tattacacgc gtaaatcaat cgaaatcgaa tatgtcgtcg acaccatgct
    12061 ggaagagaac gtgcacgata ttctctgctc ggcgctggtg gatgactgta ttgagcgagc
    12121 gaaaagtatc aagcaaggcg gcgcgaaata tgactgggtt tctggcctgc aggttggcat
    12181 tgccaacctc ggcaacagcc tggcggcagt gaagaaactg gtatttgaac agggcgcgat
    12241 tggtcagcaa cagctggctg ccgcactggc ggatgacttc gatggcctga ctcatgagca
    12301 actgcgtcag cggctgatta acggtgcgcc gaagtacggc aacgacgatg ataccgtcga
    12361 tacgctgctg gctcgcgctt atcagaccta tatcgacgaa ctgaaacagt accataaccc
    12421 gcgctacggt cgtggtccgg ttggcggcaa ctattacgcg ggtacctcgt ctatctccgc
    12481 taacgtaccg tttggcgcgc aaactatggc aacaccggac gggcgtaaag cccacacccc
    12541 gctggcagaa ggcgcaagcc cggcctccgg tactgaccat cttggcccta ctgcggtcat
    12601 tggctcagtg ggtaaactgc ctacggcagc gattctcggc ggcgtgttgc tcaaccagaa
    12661 actgaatccg gcaacgctgg agaacgaatc tgacaagcag aaactgatga tcctgctgcg
    12721 taccttcttt gaagtgcata aaggctggca tattcagtac aacatcgttt cccgcgaaac
    12781 gctgctggag gcgaaaaaac atccggatca gtaccgcgat ctggtagtgc gtgtcgcggg
    12841 ctattccgcc ttcttcaccg cgctctctcc agacgcacag gacgatatca tcgcccgtac
    12901 tgaacatatg ctgtaatctc ctcggcccgg cggcgtcgtc gccgggctaa atcattcaca
    12961 tcatcaattt catccttact ttcattcgaa atataatttg tgctctgcgt cacattgtta
    13021 ttacactgtt acggtttcat tttcgagcca ggagcacacc tatgagcgta aaagttatcg
    13081 tcacagacat ggacggtact tttcttaacg acgccaaaac gtacaaccaa ccacgtttta
    13141 tggcgcaata tcaggaactg aaaaagcgcg gcattaagtt cgttgtcgcc agcggtaatc
    13201 agtattacca gcttatttcg ttctttcctg agctaaagga tgagatctct tttgtcgcgg
    13261 aaaacggcgc actggtttac gaacatggca agcaactgtt ccacggcgaa ctgacccgac
    13321 atgaatcgcg gattgttatt ggcgagttgc taaaagataa gcaactcaat tttgtcgcct
    13381 gcggtctgca aagtgcatat gtcagcgaaa atgcccccga agcatttgtc gcactgatgg
    13441 caaaacacta ccatcgcctg aaagctgtaa aagattatca ggagattgac gacgtactgt
    13501 tcaagttttc gctcaacctg ccagatgaac aaatcccgtt agtgatcgac aaactgcaca
    13561 tagcgctcaa tggcattatg aaacccgtca ccagtggttt tggctttatc gacctgatta
    13621 ttcccggtct acataaagca aacggtattt cgcggttact gaaacgctgg gatctgtcac
    13681 cgcaaaatgt ggtagcgatt ggcgacagcg gtaacgatgc ggagatgctg aaaatggcgc
    13741 gttattcctt tgcgatgggc aatgctgcgg aaaacattaa acaaatcgcc cgttacgcta
    13801 ccgatgataa taatcatgaa ggcgcgctga atgtgattca ggcggtgctg gataacacat
    13861 ccccttttaa cagctgacct ctctaccgga gccgtgcgct ccggttttcc cttcttttta
    13921 catcctgtac atcacatcaa atcattaaac ttgcattaac ttatttataa cttatattgt
    13981 cgcatgcaaa tcagaatggt ttcgaatgaa agttaatgag aggtggttat ggcttctact
    14041 tttaccagcg acacattgcc tgccgatcac aaagcagcta tccgtcagat gaagcacgcg
    14101 ctgcgggcgc agcttggcga cgtccagcag atctttaatc agctaagcga tgacattgcc
    14161 acgcgagtgg ctgaaatcaa cgcactcaaa gcacagggcg atgccgtctg gccggtgctg
    14221 tcttatgccg atatcaaagc aggtcatgtt actgcagagc agcgcgaaca gattaaacgt
    14281 cgcggttgtg cggtgataaa aggccatttc ccccgcgaac aagcgctagg ctgggatcag
    14341 tcgatgctgg actatctgga ccgcaaccgc tttgacgagg tctacaaagg ccccggcgat
    14401 aatttcttcg ggacgctcag cgcttcacgt cccgagattt accccatcta ctggtcgcag
    14461 gcgcaaatgc aggcccgcca gagtgaagaa atggcgaatg cgcagtcgtt tctcaatcgt
    14521 ctgtggacat ttgaaagtga tggaaagcaa tggtttaacc cggatgtgag cgtcatctac
    14581 cctgaccgta tccgccgccg tccgcccgga acgacctcca aaggtcttgg agcgcatacc
    14641 gactccgggg cgctggaacg ctggctgctt ccagcgtatc agcgcgtttt cgccaacgtc
    14701 tttaatggca atctggcgca atatgatccc tggcatgcgg cacatcgtac ggaagttgaa
    14761 gagtacacgg tggacaacac caccaaatgt tccgtgtttc ggacattcca aggctggaca
    14821 gcgctctcta atatgctgcc tggtcagggg ctgctgcacg ttgtgcccat tcctgaagcc
    14881 atggcgtacg tactgttacg tccgctgctt gatgatgtgc cggaggatga actgtgcggc
    14941 gtagcgcccg gaagagtgtt gccggtatca gagcaatggc atccactgtt aattgaggcg
    15001 ttaaccagca ttccaaaact cgaggccgga gactccgtct ggtggcactg cgacgtcatc
    15061 cattccgttg cccccgttga aaatcaacag ggttggggca acgtgatgta cattcctgcg
    15121 gcaccaatgt gcgagaaaaa tcttgcctac gcgcacaagg tgaaggccgc actggaaaaa
    15181 ggcgcatcgc cgggcgactt cccgcgcgag gactatgaaa caaactggga aggacgcttt
    15241 acgcttgccg acctcaacat tcacggtaag cgagcgttgg gaatcgacag ctgagtgtcg
    15301 tacagccctt cccgttccac ggcggtgttc ccttttcctg ggcgaacccg ccgcactgac
    15361 tcccgcaccg ccaatcgtcc gtgcagcagc agcgagccca ccggagcatc cgggcgaatc
    15421 agccgctgtt gcaacatttg caccgcttca ttgcctagct catcgcgtgg cacatgcaca
    15481 gccgtcaggg gaacatcctc gattgccgca aggttaaagc cgtcgatact catcaccgag
    15541 acatcctgtg gtacgcgcag accacgacga tgtagcgcat ttaccgttcc cgccgccata
    15601 aaatccccgc cgaccagaaa cgccgtcggc aacgtcttgc cattggcggc atccagccac
    15661 gctccgacct tttcttccgc ttctttagcg ctaaaactgc tgatattcag caaatgacgg
    15721 tcatggtgaa aggccagatt atgctgacgc catgattctt taatccctgc aaaccgctgc
    15781 tccatagtgt agcggcgcag gcacatcaca ttcatcacct cccggtgacc catttcaaac
    15841 agatagcggg cggcgaacgc gccaattaac cgatgatccg gcgcaatcgc cgggaggcgc
    15901 attcgttcat cacggcagtt gatcagcaca caaggcttag cgagatcggc ggccagatca
    15961 tggatgtgcg gatcgtcaat ccccagcagg atcgctgcct gcgtatctgc ttcattcatc
    16021 cgcgagagaa agagcgcggg atcgctgtca aactcctcca gcgcgcagta acgcagccgc
    16081 acttcatgcg gcgataacgc tttgctgatg ctctggatca cacggtaata aaagatatca
    16141 gatcgttggt caaaagcgca ttgcggcgcg aaaataatca ggttgttcag caacatacga
    16201 cctgctgcca tgccatccat cacgcccaac tcacgcgcgc aggccagcac ctgttcgcga
    16261 gcgcgtgcac tggtattcgc tttgccagcc agcacccgcg aaaccgtact cggcgatagc
    16321 ccggtacgtg cggcgatatc ggcgattttc agcttcttgt ccattttgtg atctgcctct
    16381 aattcggaaa tgacagaatt ttcatgagca aaaaagccca ttctgtctgg cattaaatcc
    16441 atttcagtaa tgtatatcag tcactcatgg aaaaacgtgc acaaaacccg ctttctttcg
    16501 cttttagggt ctcttactgt gaattggtag aacaacttcc atacaaaaat aatggaaaaa
    16561 cgaacaataa agactttatt tatcaaaaag ttaaaagtgg tgtaacaact ccattctgac
    16621 gttgccgcac aaaccctata ccattctgtg gagtgcatat gagtcagggt atcaataacg
    16681 atatgactgc cagtaaaccg cgccatatcg tcaaaaatct gcgttggtgg atactggtcc
    16741 tttttttgct cggtgttact gtcaactaca tcacgcgtaa ctcgctgggt attctcgccc
    16801 ccgaactgaa aagcagccta gggattacca ccgaacaata ttcctggatt gtaggtgctt
    16861 tccagctcgc ctatacccta ttccaaccgt tgtgcggatg gctgattgac gtcattggcc
    16921 tcaaactggg ctttatgatc tgcgcgggac tgtgggcact ggcctgtctg atgcatgcgg
    16981 gggcgggaaa ctgggtacat ctggcgctgt tgcgcttctt tatgggcggt gcggaagccg
    17041 cggcaacccc agcaaacgcc aaaaccatcg gtgaatggtt tccaaaatca gaacgcccga
    17101 tcgccgcagg ctgggcaggc gtcggttttt ccatcggtgc catgctggcc ccaccgatta
    17161 tctattttgc ccacgcttca ttcggctggc agggtgcatt tatgttcact ggcatcctgg
    17221 cgatgctgtg ggtggtgctg tggtgggcgt tctatcacag cccggataag catcctaacc
    17281 tcagcaaagc cgagctggac tttattcgcc aggataacga agcgcccccg gtcaagttac
    17341 cctttctgac cgcactgaaa accgtgggca aaaataaacg cttttacggt atcgctatcc
    17401 cggcctttat ggcagaaccg gcgtgggcgg tgatgagttt ctgggtgcct ctatacctgt
    17461 ccaaaaccta tggcatggac ctgaagcaga tcgcattatt cgcctggttg ccgttcctcg
    17521 ccgctgatct tggcagtatt gccagcggct atctgaccaa actttacacc cgcctgtttg
    17581 ggctgagtcg tgtgaactcc gttgtcgcat catcggtcag tggcgcattc ttgatggttt
    17641 cgctggcggt gatggccttt accaataacc cgtatatcgc catcgtcctt atctccatcg
    17701 gcggcttcgg tcaccagatc atctcctgca tgctgagcgc actggtggtg gaatccttcg
    17761 acaaaggtca gatggccact gttaacggca tgcgcggttc tgccgcgtgg atcgcaagtt
    17821 tcctgttctc cctgattatc ggggttaccg ctgacaaaat cggctttaac cccctgttta
    17881 tcgccatggg cttctttgac ctgattggcg ctttcttcct ggtcaccttt attgctgaac
    17941 gtcgcgcaaa acgcgcctga gagtggatgt aaacatgaaa acgctgaaaa actggaccct
    18001 gcacaaacag tctgcccacc acgttgaact gctggtcgat ggccagcata tcctgtgcct
    18061 gtacgtgctg gaagaaaatc tgttccgcgt gctgttaaaa cgcaaaggcg agctggcgct
    18121 agatcgcacc tggagcattg ctccggcgca ggatgtgcca tgggaagggc gcagccgcga
    18181 agatctcagc ggctttagcc tgcccgcgtg gacagtgatc cagcacgacg acaccgtggt
    18241 tatcgccacc gagtcactgc gtgcaaccgt tcaccagccg ctgtggctgg agtggcacta
    18301 tcgtaacaac gaaggcgaat ggctgccgtt ggtgaatgac cgcccgacca gcgcttatct
    18361 gctgaatgcc cacggcgatg gcgtggcaca ctatcagagc cgtcgtaaag atgaacgctt
    18421 ttatggcctc ggagataaag ccggcgatct gcaacgcaac ggtaaacgtt acgagatgcg
    18481 taacctcgac gcgatgggct acaacgccgt tagtactgac ccgctgtaca aacacattcc
    18541 attcaccatc acccgccgcg atgacattag cttcggcctg ttttatgaca acctcagcag
    18601 ctgctggctg gatctgggca acgagatcga caactaccac accgcctatc gccgctggca
    18661 ggcggaagcg ggtgatatcg actactacct gtttaccggc gaacgcgtac ttgatatcac
    18721 caaagccttc gtacgcctga ccggaaaaac cctgttcgga ccgaaatgga gcctgggcta
    18781 cagcggttca actatgcact acaccgatgc gccggacgca caaaatcagt tgatgaactt
    18841 tatccgcctg tgtgaagaac atgcaattcc ctgcgattcg ttccagctct cttccggtta
    18901 tacctcaatt aacggcaaac gctatgtgtt caactggaac tacgacaaag tgccgcagcc
    18961 gaaagtgatg agccaggcat ttcacaatgc tggtttgcac ctggccgcca atatcaagcc
    19021 gtgcctgttg caggatcacc cacgctataa cgaagtggca gaaagaggac tgttcatccg
    19081 tgattcagaa acagatgaac cagaacgttc cagcttctgg gatgacgaag gttctaacct
    19141 cgactttact aatccgcaga ccatccagtg gtggcaggaa ggcgtgacca cacagcttct
    19201 ggagatgggt atcgactcca cctggaacga caacaacgaa ttcgaagtat gggacggtga
    19261 agcacgctgt cacggctttg gtcagacgat cgcgatcaaa catattcgcc cagtgatgcc
    19321 actgctgatg atgcgagcct cgatggaagc ccagcagcgt ttcgctccgg aaaaacgtcc
    19381 gtacttgatc tcccgctccg gctgcgcggg aatgcagcgc tacgtgcaga cctggagtgg
    19441 cgataaccgc actagctggg acactctgcg ctacaacacc cgcatgggtc tggggatgag
    19501 cctctccggg ctgtataacc tcggtcacga cgtcggcggt ttctccggtg ataagccgga
    19561 cccggaactg tttgtacgct gggtacaaaa cggcgtgatg catccgcgct ttacgattca
    19621 ctcatggaac gatgaccata ccgtaaacga accgtggatg tatccgggcg tcactccggc
    19681 gatccgcagc gctattgagt tgcgctatcg cctgctgccc tacttttata ccctgatgtg
    19741 gcaggcatac gccgatgatg aaccaatgct acgtccgact ttccttgacc acgagcacga
    19801 cgttcagact tttgaagagt gtgatgactt cctgcttggt cgcgatattt tggttgccag
    19861 cgtcgtagag caaggcgagc gccagcgccg tgtatggctg ccagataacg aaactggctg
    19921 gtacgatttc tacaacggcg aatggttcag tggcggtcag tggatcaccc tcgacgcacc
    19981 gctggagaaa ctgccgctga tggttcgtgc cggggctggt ctaccgctca gcaaacgcat
    20041 cacctatgtg agcgcagaac aagacgatac ccgcgaactg aaactgttcc cgctgaaagg
    20101 tgtgggcaca acgtccggcc tgctgtttga agacgatggc gaaagctggg gctaccaaac
    20161 tggtaatgcg ctttgggtgg aatgggaaat ggtttgcgat ggggcgacca tcaacctgaa
    20221 agtcaacgcg cgcggggatt atcgtcctgc atggagtgca ctgaaagtgt cattaccatt
    20281 ggaagaaaaa cgcacgctgc tggtcaatgg tgttgaaggg agtgaatgga tgcgttaaat
    20341 cattaccggg gccgggaggc gactgcacct tacccggtcc agccatcgaa tcctttacct
    20401 cagtatttca cgctgcagga aggcagcaaa cctgtaaacc ccgggagctt aaataagtaa
    20461 gtgaccgggg tgaacaggtg aagccaatgc atctgccgca tgaaagacgg cggggaatta
    20521 agcgtcgatc cctttactac gcaggtaatc ttcgtaatta ccgctaaagt cgatcgcgcg
    20581 ttccggggtg atttccagaa tgcgggtcgc cagggagctt acgaactcac ggtcgtgtga
    20641 aacaaagatc agcgtgccct gatacagttc cagcgccatg ttcagtgact cgatggattc
    20701 catatccagg tggttggtcg gttcgtccat gatcagaatg ttcggcttct gcatcattaa
    20761 cttaccaaac agcatccgcc ctttttcccc accggaaagc actttagctg gctttttgat
    20821 gtcgtcctgg ctgaacagca aacgaccgag aatgctgcgt accgcctgct cgtcatcgcc
    20881 ttcctgcttc cactgactca tccattcgaa cacggtcaga tcattttcaa actcatattc
    20941 gtggtcctgg gcgtagtaac caatgcgcgc gttttcagac cattttacgg tgccgctgtc
    21001 cggttgcaga tcgcccacca gcgttttcag cagcgttgat ttaccgacgc cgttggtacc
    21061 cagtaccgca agtttttcac cgacctccag cagcaggttg agatttttaa acagtggacc
    21121 gttatcaaac cctttggtca gaccttccac ttccagcgcg ttacggaaca gtttcttatc
    21181 ctgttcaaaa cggatgaacg ggttctgacg gctggaggct ttcacctctt ccagtttgat
    21241 tttatcaatc tggcgcgcgc gcgaagttgc ctggcgagat ttcgaggcgt tggcgctaaa
    21301 gcggctaacg aaagattgca actcagcaat ctgcgctttc ttcttggcgt tatcggccag
    21361 cagacgttca cgcgcctggg tcgccgccgt catgtactca tcgtagttac ccggataaac
    21421 gcgcagctcg ccgtaatcca gatccgccat gtgggtacag accatgttaa ggaagtgacg
    21481 gtcgtgcgag atgatgatca tggtgctgtc acgctcgttc agcacctgtt ccagccagcg
    21541 aatggtgtcg atgtcgaggt tgttggtcgg ttcgtcgagc aggagaatat ccggatcagc
    21601 aaacagcgcc tgcgccagaa gcacacgcag tttccagcca ggagcaactt cactcatcgg
    21661 gccgtagtgc tgttccactg gaattcccac gccaagcaac agttcaccgg cgcgagcttc
    21721 cgcagagtaa ccgtccattt cgccgtattt aacttccaga tcggccactt tatagccgtc
    21781 ttcctcactc atttccggca aagcatagat gcggtcacgc tcctgcttca cttcccacaa
    21841 ctctttatgc cccatgatca ccgtatccag cacagtgaac tcttcaaagg caaactgatc
    21901 ctgacgcagc ttaccaatgc gctcgttggg atcgagggaa acgttaccca aagtcggctc
    21961 taagtcgccg ccgaggatct tcataaaggt ggatttacca ctaccgttcg cgccaatcag
    22021 gccgtaacgg ttgccgccgc cgaatttgac ggaaatgttt tcaaacaacg gcttactgcc
    22081 gaactgcatg gtgacgttac tggaaactaa cacaggcgta tcctgaaaag agatatgaca
    22141 aaccgcgcat tatgccataa gtctgaaaga agatcctgct cctcgcttaa ggtctacact
    22201 caagccaaag gcaaaaaatg tgatttcgta cacatctgat ttcactgtga gctggaatga
    22261 acttataatg cgcttccaat actctaatat tctcaaccca atggcctgcc aggcacaaaa
    22321 tctcgcttaa catgaatatg aaattgaaaa cattattcgc agcggccttc gctgttgtcg
    22381 gcttttgcag taccgcctct gcggtaactt atcctctgcc aaccgacggg agtcgcctgg
    22441 ttggtcagaa tcaggtgatc accattcctg aaggtaacac tcagccgctg gagtattttg
    22501 ccgcggagta ccagatgggg ctttccaata tgatggaagc gaacccgggt gtggatacct
    22561 tcctgccgaa aggcggtact gtactgaaca ttccgcagca gctgatcctg ccggataccg
    22621 ttcatgaagg catcgtcatt aacagtgcag agatgcgtct gtattactat ccgaaaggga
    22681 ccaacaccgt tatcgtgctg ccgatcggca ttggtcagtt aggcaaagat acgcctatca
    22741 actggaccac caaagttgag cgtaagaaag caggcccgac ctggacgccg accgccaaaa
    22801 tgcacgcaga gtaccgcgct gcgggcgaac cgcttccggc tgtcgttccg gcaggtccgg
    22861 ataacccgat ggggctgtat gcactctaca tcggtcgcct gtacgctatc catggcacca
    22921 acgccaactt cggtatcggc ctgcgtgtaa gtcatggttg tgtgcgtctg cgtaacgaag
    22981 acatcaaatt cctgttcgag aaagtaccgg tcggtacccg cgtacagttt attgatgagc
    23041 cggtaaaagc gaccaccgag ccagacggca gccgttatat tgaagtccat aacccgctgt
    23101 ctaccaccga agcccagttt gaaggtcagg aaattgtgcc aattaccctg acgaagagcg
    23161 tgcaaacagt gaccggtcag ccagatgttg accaggttgt tcttgatgaa gcgattaaaa
    23221 accgctccgg gatgccggtt cgtctgaatt aatcttcaaa acttaaagca aaaggcggac
    23281 tcataatccg ccttttttat ttgccagacc ctagttggcc gggagtataa ctaacaaaac
    23341 atatcccacc aacgccgccc acaacagcat cggtattgaa tagagatgga aacgccacca
    23401 gatgcggcga tcgttcgcca tacgtagcgc aatcaaattt gccagcgatc cgggtaataa
    23461 cccaaagcca cctacgttta ccgcccatac cagtaataaa gacggcggca catagttcag
    23521 caacaatatg gtactcggca cgttactgat cacctgcgat aaaccgattg ccgttaacca
    23581 taacccgggt tcagatagat gactcacgtt acccaacacg ccttgcaacg ctggaagctg
    23641 ggtcagtaaa tggacgtcga taaacatcgc cataaacacc agcagcagcg tccagtccac
    23701 actgagcacc acgcgacgtg ccagcagcgc aaagcctgcc gccacaatca ccagtcccca
    23761 cagctcttgt ttgaactcca gcgccgtcag aaagacgata tacagcccca gacaactcca
    23821 caccagccgc ggtttccact ccggtgtttg cacccccgta tggtattgca gcgcctttcc
    23881 agggaaacaa caccagcaca ggagcaggag cgtcagcatc attgcgccag ccagcggtgc
    23941 catttgggca ataaatccgg caaacgaaag accagaacgt ccccagataa gaatattttg
    24001 cgggttgcca attggcgtca gtagcgaacc agcgttgact gccagcgcct caaaaataat
    24061 cagccgatta accgggatct cacacagtct ttttagcgtg atagtcagcg gaacaacaat
    24121 aaacagcgcg acatcgttgg tcagaaaggt agaaagcagc gccgccgcca gcaccataaa
    24181 catcgccagc cgacgctccg tagcaaagcg gcgcaccatt ttgcgcccca gcacatcaaa
    24241 ataaccgctt aactccacac ctttggtcag cagcatcagg ccgcttaagg tgatgatggt
    24301 gtgccagtcg atagcagcag gccaggattt cggtgcaaag ggcacgaaaa agcttaatcc
    24361 gataccaaca agaattaata actgaaaaaa acgatcgcct tgcagcgtgc gtaaaaaagg
    24421 caggctcatt tggcaccgtg tttctgggta aacaaacgaa aagcgtcgag cgtctcttca
    24481 ctaacatggt gctccatgcc ttccgcgtcg cgacgggcga tttccggact gacgcccaac
    24541 accaacaaga aattttcgac tatctgatgt cgctcgcggc tttcctgcgc cagcttctct
    24601 ccttctgccg ttaaaaacac gccacgccag gggatcattt caatcagccc catggttgcc
    24661 agccgcttaa gcattttagc caccgtcggt tgcgaaactc ccagacgagc agccatgtcc
    24721 acctgacgag cttcccccac ttccctgatc aagtcagaaa tcagctcaac gtaatcatca
    24781 ataagctcgc gccgatgcgc ctctctgacc tggcggaacc cttcaacgtg ctcttccacg
    24841 ttcactaact gcgtcacttt ttttgctgtt ggcgtacctg cgcgacgact cattgtgctt
    24901 cctcattatg gtgacgcata catagcgttc tcatttcgtg cgcacattgt aaaccagagt
    24961 tgcgaaggta caaaaaatta acgttttagc aatagctaca taatatagcc tgtgctatat
    25021 ctgtatgtaa tgcaatcatc cctcaaggat cgacgggatt agcaagtcag gaggtcttat
    25081 gaatgagttc aagaagtgta tgcgcgtgtt tagtcattct ccctttaaag tacggttaat
    25141 gctgctctct atgttgtgcg atatggtcaa caacaaaccg cagcaagata aaccttccga
    25201 taaatagcgg cgtcgcggta cgccgcttca ctcctgcttt tatgcaggca taacgcgttt
    25261 tggtctgaaa aaccccactt tttgtcggat ttgcaatccc cttcgcaaaa gatttgttcg
    25321 tcagtagttg acctgaacgg cggctcgctc tatcttcttg cagccctgcg tatattgcgg
    25381 ctcgcggatg cggacccctt tcccctcttc acgcactctt gcaggtattg acccttgacg
    25441 ccagggtaag cacatggcgt ttgttacgat agtggcatat gaatttaacc ctcaaagaat
    25501 cgcttgttac ccgtagccgg gtatttagcc cgtggactgc gttctacttt ttacagtcgc
    25561 tattaattaa cctcggctta ggttacccct tcagtttgct ctacaccgct gcgtttacgg
    25621 ctattttgct tttgctatgg cgaacattgc ctcgcgtaca aaaagttctg gtcggtgtca
    25681 gttcgctggt ggcggcttgt tatttccctt ttgctcaggc ctacggcgcg cctaatttca
    25741 atacattgct ggcattgcac tccaccaata tggaagagtc gaccgaaatc ctgacgattt
    25801 ttccgtggta cagctacctg gtcggcttat ttatttttgc gctcggcgta atagcaatca
    25861 ggcgaaaaaa agagaatgaa aaagcgcgct ggaatacctt cgacagcctg tgtctggtat
    25921 tcagtgtggc gacatttttt gttgctcccg tgcaaaacct ggcctggggt ggcgtattta
    25981 aactgaaaga tactggctat ccggtatttc gttttgctaa ggatgtcatc gtcaataata
    26041 acgaggtgat tgaagagcaa gaacggatgg caaaactttc cggaatgaaa gatacctgga
    26101 cggtcactgc cgttaagccg aagtatcaga cctatgtggt ggtgatcggc gaaagcgcgc
    26161 gtcgcgatgc cctcggtgcc tttggcggtc actgggacaa taccccgttt gccagcagcg
    26221 ttaacggttt gatatttgct gactacattg ccgccagtgg ctccacgcag aaatcacttg
    26281 gcttaacgct caatcgcgta gtcgatggca aaccacagtt tcaggataac tttgtcaccc
    26341 tggcaaatcg cgcgggcttc cagacctggt ggttttccaa ccagggccaa atcggcgaat
    26401 acgatactgc tatcgccagc atcgccaaac gtgcagatga agtgtacttc ctgaaagaag
    26461 gtaattttga agcagataaa aacacgaaag acgaagcgtt actggatatg accgctcaag
    26521 tgctggcgca agagcactcg caaccgcagc tgattgttct gcatctgatg ggctcgcatc
    26581 cgcaggcctg cgacaggaca caaggcaaat acgaaacctt tgtgcaatcg aaagaaacgt
    26641 cgtgctatct ctataccatg acgcaaacgg acgatttact gcgcaagctg tacgatcagt
    26701 tacgcaacag cggcagcagc ttctctctgg tttacttttc tgaccacggt ctggccttta
    26761 aagagcgcgg caaagacgtg caataccttg cccatgatga taaatatcag caaaatttcc
    26821 aggtgccttt tatggtcatt tccagcgacg ataaagcgca tcgggtgatt aaagcccgcc
    26881 gctcagccaa tgacttctta ggctttttct cgcagtggac gggaattaaa gcgaaggaaa
    26941 taaatatcaa atacccgttt atatctgaga agaaagccgg gccgatatac atcaccaact
    27001 tccagttaca gaaggtggat tacaaccatc tcggaaccga tattttcgac ccgaaacctt
    27061 aaaacaaaaa atccgccccg agaggcggat tttttatatc accaaagtga ttagaagcgg
    27121 taaccaacac cggcaatcca ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa
    27181 gagaagtcca gagcaacgtt ttccatcggg ttgaactgca gaccagcacc gtaggagaaa
    27241 ccgtagtcgc tggtgtcgtg tttgtaggtc gggtattcag tggtctggaa tttaccataa
    27301 cccaccccca ctacaccgta gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca
    27361 gtgatgccgt agtactggtt tttgttgtag tcaccagagc ttgcagtacg gcttttctcg
    27421 gtgtaagtga aagaaccgat cacacccagc gggctgttgt cttcttcata gcggtatttc
    27481 aggttgaaac cgcccatttt gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca
    27541 gttacggtag aagtcgcagc tacggaagta cctgcggtga aagccagaac tgcggccagt
    27601 gctgaaagac atgcaatttt tttcataacc acctcaaatg tgattcaaat aagtcctaag
    27661 ttttaaatat atcaaaaatt aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca
    27721 tttcatgtaa cagaacgttt ccatacaccg ctatccatct aaatttaaat cactttttca
    27781 gagaactgcg taagtattac gcatgttttc cctgtcattc atccagatta ttcctaatca
    27841 ccagactaat gattccatca atcctggcgc attttagtca aaacggggga aaattttttc
    27901 aacaaatgct caaccagcat tgggtatatc cagtacactc cacgctttac ttaagtctag
    27961 atatttgtgg gagaaaggat gcctggttca ttacgtaaaa tgccggtctg gttaccaata
    28021 gtcatattgc tcgttgccat ggcgtctatt cagggtggag cctcgttagc taagtcactt
    28081 tttcctctgg tgggcgcacc gggtgtcact gcgctgcgtc tggcattagg aacgctgatc
    28141 ctcatcgcgt tctttaagcc atggcgactg cgctttgcca aagagcaacg gttaccgctg
    28201 ttgttttacg gcgtttcgct gggtgggatg aattatcttt tttatctttc tattcagaca
    28261 gtaccgctgg gtattgcggt ggcgctggag ttcaccggac cactggcggt ggcgctgttc
    28321 tcttctcgtc gcccggtaga tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc
    28381 ctgctaccgc tggggcaaga cgtttcccat gtcgatttaa ccggctgtgc gctggcactg
    28441 ggggccgggg cttgttgggc tatttacatt ttaagtgggc aacgcgcagg agcggaacat
    28501 ggccctgcga cggtggcaat tggttcgttg attgcagcgt taattttcgt gccaattgga
    28561 gcgcttcagg ctggtgaagc actctggcac tggtcggtta ttccattggg tctggctgtc
    28621 gctattctct cgaccgctct gccttattcg ctggaaatga ttgccctcac ccgtttgcca
    28681 acacggacat ttggtacgct gatgagcatg gaaccggcgc tggctgccgt ttccgggatg
    28741 attttcctcg gagaaacact gacacccata cagctgctgg cgctcggcgc tatcatcgcc
    28801 gcttcaatgg ggtctacgct gacagtacgc aaagagagca aaataaaaga attagacatt
    28861 aattaaattt acatttctgc atggttatgc ataaccat
//
LOCUS       DAZRUF010000022        28204 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZRUF010000022 DAZRUF010000000
VERSION     DAZRUF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28204
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.022"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..468
                     /locus_tag="ACHXOL_000811"
     CDS             <1..468
                     /locus_tag="ACHXOL_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6807866.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="ACHXOL_000812"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="ACHXOL_000812"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR6807867.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="ACHXOL_000813"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="ACHXOL_000813"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR6807868.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHXOL_000814"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHXOL_000814"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR6807869.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="ACHXOL_000815"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="ACHXOL_000815"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR6807870.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="ACHXOL_000816"
     CDS             complement(4084..5313)
                     /locus_tag="ACHXOL_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR6807871.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="ACHXOL_000817"
     CDS             complement(5377..5817)
                     /locus_tag="ACHXOL_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR6807872.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="ACHXOL_000818"
     CDS             complement(5922..6692)
                     /locus_tag="ACHXOL_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR6807873.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="ACHXOL_000819"
     CDS             complement(6689..7615)
                     /locus_tag="ACHXOL_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR6807874.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="ACHXOL_000820"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="ACHXOL_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR6807875.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHXOL_000821"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHXOL_000821"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR6807876.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="ACHXOL_000822"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="ACHXOL_000822"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR6807877.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHXOL_000823"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHXOL_000823"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR6807878.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEIPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="ACHXOL_000824"
     CDS             13322..13669
                     /locus_tag="ACHXOL_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR6807879.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="ACHXOL_000825"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="ACHXOL_000825"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR6807880.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="ACHXOL_000826"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="ACHXOL_000826"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR6807881.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHXOL_000827"
     CDS             complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHXOL_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR6807882.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHXOL_000828"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHXOL_000828"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR6807883.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="ACHXOL_000829"
     CDS             18978..20735
                     /locus_tag="ACHXOL_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR6807884.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHXOL_000830"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHXOL_000830"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR6807885.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHXOL_000831"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHXOL_000831"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR6807886.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHXOL_000832"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHXOL_000832"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR6807887.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHXOL_000833"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHXOL_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR6807888.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         6797 a         7676 c         6935 g         6796 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcaagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtattt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgt
//
LOCUS       DAZRUF010000023        28107 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZRUF010000023 DAZRUF010000000
VERSION     DAZRUF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.023"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHXOL_000834"
     CDS             complement(<1..215)
                     /locus_tag="ACHXOL_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR6807889.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHXOL_000835"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHXOL_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR6807890.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHXOL_000836"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHXOL_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR6807891.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHXOL_000837"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHXOL_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR6807892.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHXOL_000838"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHXOL_000838"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR6807893.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHXOL_000839"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHXOL_000839"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR6807894.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHXOL_000840"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHXOL_000840"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR6807895.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHXOL_000841"
     CDS             complement(4604..5326)
                     /locus_tag="ACHXOL_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR6807896.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHXOL_000842"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHXOL_000842"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR6807897.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHXOL_000843"
     CDS             6526..6744
                     /locus_tag="ACHXOL_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR6807898.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHXOL_000844"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHXOL_000844"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR6807899.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHXOL_000845"
     CDS             complement(7478..7678)
                     /locus_tag="ACHXOL_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR6807900.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHXOL_000846"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHXOL_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR6807901.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHXOL_000847"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHXOL_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR6807902.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHXOL_000848"
     CDS             10175..12088
                     /locus_tag="ACHXOL_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR6807903.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHXOL_000849"
     CDS             12088..13110
                     /locus_tag="ACHXOL_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR6807904.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHXOL_000850"
     CDS             13104..13322
                     /locus_tag="ACHXOL_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR6807905.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHXOL_000851"
     CDS             13376..14245
                     /locus_tag="ACHXOL_000851"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR6807906.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHXOL_000852"
     CDS             complement(14300..14704)
                     /locus_tag="ACHXOL_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR6807907.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHXOL_000853"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHXOL_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR6807908.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGSR"
     gene            15689..17779
                     /locus_tag="ACHXOL_000854"
     CDS             15689..17779
                     /locus_tag="ACHXOL_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR6807909.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHXOL_000855"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHXOL_000855"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR6807910.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHXOL_000856"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHXOL_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR6807911.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHXOL_000857"
     CDS             complement(19747..20349)
                     /locus_tag="ACHXOL_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR6807912.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHXOL_000858"
     CDS             complement(20339..20506)
                     /locus_tag="ACHXOL_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR6807913.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHXOL_000859"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHXOL_000859"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR6807914.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHXOL_000860"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHXOL_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR6807915.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="ACHXOL_000861"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="ACHXOL_000861"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR6807916.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="ACHXOL_000862"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="ACHXOL_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR6807917.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="ACHXOL_000863"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="ACHXOL_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR6807918.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cysG"
                     /locus_tag="ACHXOL_000864"
     CDS             26714..28087
                     /gene="cysG"
                     /locus_tag="ACHXOL_000864"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR6807919.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6788 a         7559 c         7114 g         6646 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggct ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt cgccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaat
//
LOCUS       DAZRUF010000024        28050 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZRUF010000024 DAZRUF010000000
VERSION     DAZRUF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28050
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.024"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..248
                     /gene="tnpA"
                     /locus_tag="ACHXOL_000865"
     CDS             <1..248
                     /gene="tnpA"
                     /locus_tag="ACHXOL_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFR6807920.1"
                     /translation="MDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRPDIAV
                     RYYYKGVLWSPGYFANSCGGAPISVIRQYIEQQQTPG"
     gene            308..382
                     /locus_tag="ACHXOL_000866"
                     /pseudo
     CDS             308..382
                     /locus_tag="ACHXOL_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(379..1702)
                     /locus_tag="ACHXOL_000867"
                     /pseudo
     CDS             complement(379..1702)
                     /locus_tag="ACHXOL_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1702..3318)
                     /locus_tag="ACHXOL_000868"
     CDS             complement(1702..3318)
                     /locus_tag="ACHXOL_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR6807921.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3614..4330
                     /locus_tag="ACHXOL_000869"
     CDS             3614..4330
                     /locus_tag="ACHXOL_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR6807922.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4327..5988)
                     /locus_tag="ACHXOL_000870"
     CDS             complement(4327..5988)
                     /locus_tag="ACHXOL_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR6807923.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHXOL_000871"
     CDS             complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHXOL_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR6807924.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHXOL_000872"
     CDS             complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHXOL_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR6807925.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7131..7203)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7443..7775
                     /locus_tag="ACHXOL_000873"
     CDS             7443..7775
                     /locus_tag="ACHXOL_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR6807926.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7777..8991)
                     /locus_tag="ACHXOL_000874"
     CDS             complement(7777..8991)
                     /locus_tag="ACHXOL_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR6807927.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHXOL_000875"
     CDS             9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHXOL_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR6807928.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10153..11490)
                     /locus_tag="ACHXOL_000876"
     CDS             complement(10153..11490)
                     /locus_tag="ACHXOL_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6807929.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHXOL_000877"
                     /pseudo
     CDS             complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHXOL_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHXOL_000878"
     CDS             complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHXOL_000878"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR6807930.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHXOL_000879"
     CDS             complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHXOL_000879"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR6807931.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHXOL_000880"
     CDS             complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHXOL_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR6807932.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15153..15809
                     /locus_tag="ACHXOL_000881"
     CDS             15153..15809
                     /locus_tag="ACHXOL_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6807933.1"
                     /translation="MKLNLKGFFKTAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLKDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYNWSTEEEKARVTQYRFACGVQHCTRTVENLEGTIHEKNKN
                     MDYSNVMAFYHPFKVRFYEYHSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHXOL_000882"
     CDS             complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHXOL_000882"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR6807934.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16782..17180)
                     /locus_tag="ACHXOL_000883"
     CDS             complement(16782..17180)
                     /locus_tag="ACHXOL_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR6807935.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHXOL_000884"
     CDS             complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHXOL_000884"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR6807936.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHXOL_000885"
     CDS             complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHXOL_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR6807937.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHXOL_000886"
     CDS             complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHXOL_000886"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR6807938.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHXOL_000887"
     CDS             complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHXOL_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR6807939.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23741..23821
                     /locus_tag="ACHXOL_000888"
     CDS             23741..23821
                     /locus_tag="ACHXOL_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6807940.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHXOL_000889"
     CDS             24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHXOL_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR6807941.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHXOL_000890"
     CDS             24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHXOL_000890"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR6807942.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24531..24788
                     /gene="yidD"
                     /locus_tag="ACHXOL_000891"
     CDS             24531..24788
                     /gene="yidD"
                     /locus_tag="ACHXOL_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR6807943.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24791..26437
                     /gene="yidC"
                     /locus_tag="ACHXOL_000892"
     CDS             24791..26437
                     /gene="yidC"
                     /locus_tag="ACHXOL_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR6807944.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHXOL_000893"
     CDS             26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHXOL_000893"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR6807945.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6760 a         7571 c         7159 g         6560 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      181 atagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccgc gccttatatc cccgccctga gggacggggt tttacggcgc
      301 accggataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac
      361 ggatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa
      421 ctcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgata acgtaatcgc
      481 ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc
      541 cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc
      601 tggtccgttg tgcccgacca gacacgggat ctcgaccatc gcttcatcat caaagttatg
      661 gattgccccg ttgttaggca caatcagcaa catccgctcc tgagtgttga aggcaattgc
      721 cgccgccaga tcgacgatgt atgacgcatg ttcgtcaatc tccagcttgg ccgccgctga
      781 atttcctgcc gctgtaatgg cgcggcaagc atcgaaaacc tgtttctcgc gatgttccat
      841 tcacctcatt cgcgcgggta tgttcagggt tggaatgctg aacaacataa tccgggaaga
      901 gataatattt cagataggtg ttcggcaatg tatccgggtc cagtgcctgg acatcccgcg
      961 cttttgcata ggtgtcgttc cagctcgctt cagtatgtgt atcttgctgc ggtggaacat
     1021 aaccgtgctt cgagacatat tgccgcagct ggggcatcag gtcgttgccc tgcaaatctt
     1081 caattgatgt ccaccagcca aagtggttca ggccgtagta gcgcacgcgc atctgtttgc
     1141 gatcttgcag cccaacaatt tgcgccatcc ggctttcaat accgattggc atgtcacaga
     1201 tgttgaggat tttcgcattc gggcgcagac gacgcgtggc ttctgcgaca atggctgccg
     1261 ggttggagta gttgagcatc caggcatttg gtgaatattt ttccatataa tccaccagtt
     1321 ccaggacgcc gccaatggaa cgcatgccgt acgctattcc gccgggtccg caagtttcct
     1381 gaccaacaac accgtggcgc agtgggattt tttcatccag ttcgcgcatc gggtacttgc
     1441 cgacgcggat gtgcgccatg acaaaatcaa cgtcgctgaa cgccacttca ggatcggtgg
     1501 tgtaactaaa cgcaatgtcc ggcgctttct ctttaaggat gactttacag gcttcggcaa
     1561 tcacttcctg ccgcgcgcca tcgttatcat aaaatttcag tgcacgaagc gggaaacgct
     1621 cctgattcgc caggagcatc aacacgatgc ctggcgtaaa ggtgcttcca ccgcctgcga
     1681 caaccactga gaatttggtc attatactgc ctccgtaatg gcaacatttt ctgctgattg
     1741 atgagaatta attaagctat cgagctgttc acgcagctgg gatacatgca gaccaatgat
     1801 cacctgaatg gcatcgccac tacggaagac gccgtgcgct cccagctttt taaagacttc
     1861 gtcatccagc gtttgtgaca tgtcatgcag tgcaatgcgt agacgcgtcg cacaattgtt
     1921 aatgctggag atattgccga ccccgcccag ggcttgcagg ataccggcag cttgatccag
     1981 ctcttttttt ggctctgccg cggtggtttg gcctcgcgag gctttgtatt cggcttttga
     2041 gtagagtttc acttccgcat cttcacgtcc cggcgtgcac atgttgaact gcagaatcag
     2101 ggtgcggaaa accacgaagt acagcagggt aaagcacaac ccaatggcga tttgggtcag
     2161 catcatatcc gcgtggttgc tgaacatcgg gatccagttt tgcggtaaaa cctggtcaat
     2221 cagacctccg cccatgttgc ccaccacacc aaagagatac atcacggtcg acattgaggc
     2281 cgcaagcaca gcgtgtaccg caaacagcaa cggtgaaatg aacaggaagg taaattccag
     2341 cggttcggta attcccacca gcatggcggt taaggttgcg ggaatcagca agcccgccac
     2401 ttttacccga ttttccggtg cggcagtgaa gtacatcgct aaagaaatgc ccacggcacc
     2461 aaagattttt gagttaccgt gcagggcaaa accgccttcc gggaacaacg atttcagcgg
     2521 ctcggcgctc aaactgaact cttgcagatg ctgcgcccag tacatctgaa tgccgccttc
     2581 aacagctgcc ggacccaaga taaacggtcc gtagatgaag tggtgtaaac cggttgggat
     2641 cagaatacgt tcgaggaagg tgtaaaccca gaccccaagc gcacccgccg aacgcaggaa
     2701 cgcttgcaga gattcaatcc ccgtttgtac ttttggccag ccgagcaatg tcagccaggc
     2761 acaggggatc atcaccagga aggcgataat caccacataa gacgtcccct ggaaaatgcc
     2821 gagaaaaacc ggcagttttt tatcgaacag acggttatgc agcgccgtca caatgccgga
     2881 aatgataatt gcgccgataa tgctggtatc gagggtttta atcccggcca tcattgtcag
     2941 accgctacct gccaccgcgt cctgagtgaa atcgacgccg aagtagcttc cccaggtcat
     3001 tcccatcgcg ttgatgaaat agttccaggt caggaaactc accatcaccg ccagacaagc
     3061 acgtccctgc gcttgcttag caaggccaat gggtaaaccg acagcaaaaa tcagcggcat
     3121 attacggaat accgtccaac cgccctcttc aataatgtgt acgatttgcg cgaataaact
     3181 gttcggatcg gtcagtgatt ccccgacaaa catcgggttt tgcagcaaga tggcaagacc
     3241 caccacaatc ccggcgaagg gaaacagcag cactggcgta aacatcgcgc cgccaaagcg
     3301 ttgaatttga ctgagcattt ccacatcctc ttttctcaat tctgtaagta ggaagcctga
     3361 ttggttatgc ggcaggcctg gagcaaggat aggaagatgc gatcggcaga acatgacgac
     3421 ccaatgggat tcgtgatcgc tttcatgctt ttcgttgggc ggtagagatc tacgttttcg
     3481 cgaaaaagta gacatctgtg agcacaggta cacagcatcg aacacatctt taaaaaaaag
     3541 atgttttttc aatcgattaa gcagaacttg tgtgcgcatt acccgggctt gcaggcaaaa
     3601 aagagatcta gagatgatct acaaaagcat tgcggagcgg ttaagaattc gacttaactc
     3661 cgcagatttc acgctaaaca gccttcttcc gggtgaaaaa aagctggcgg aagagtttgc
     3721 ggtatcgcgg atgaccatcc gtaaagccat tgacctgctg gtagcgtggg ggctggtggt
     3781 ccgccgccac ggtagtggca cttacctggt gcgcaaagat gtactgcatc aaaccgccag
     3841 tctgaccggg ctggtggagg tgttaaaacg gcagggaaaa actgtcacca gccaggtgct
     3901 gatttttgaa atcatgcctg cgcctccggc cattgccagc cagttacgga ttcaaatcaa
     3961 cgagcagatc tacttctccc gtcgcgttcg ttttgtggaa gggaaaccgc tgatgctgga
     4021 agacagctat atgccggtaa aactgttccg taatctttcg ctgcaacatc tggaagggtc
     4081 gaagtttgaa tatattgaac aagagtgcgg gattttgatt ggcggtaatt atgaaagcct
     4141 gacgccggtg ctcgccgata gactgctggc gcggcaaatg aaggtagcgg aacacacgcc
     4201 actgctgcgg atcacctcgt tgtcatatag cgagagcggg gagtttttga attattcagt
     4261 gatgttcaga aatgccagcg aataccaggt ggagtaccat ttacggcgac tccacccgga
     4321 aaagagttaa ccgatactcc agaagagcac cgccagtaat tggggggtga taatgcgcag
     4381 gaacatcact aacggataga cagtggcgta agagagcgcc gccgcaccgc tagttggatg
     4441 aagattatta gcaaacgcca gcgccggagg atcggtcatg gaacctgcca gcatcccgca
     4501 catggtcagg taattcattt tggctaacat ccgcgccaga atgccaacag taatcagcgg
     4561 aacggcggtg atcagggcac cataaccaat ccagcttagc ccttcgccat tgaccagggt
     4621 attcacaaaa tccccaccag atttcagacc aacgaccgag aggaacagca cgatccccag
     4681 ctcccgcagc gcgaggttgg cacttggcgg cataaaccag tacagcttgc cgatactgcc
     4741 gatacgcccg aggatcaacg ccataatcag cagaccgccc gccagcccca gtttcaacgc
     4801 ggccgggaat cccggcacaa agacgggaat agaacctaac aatacgccaa gcccgatgcc
     4861 aataaacact ggcagcatct gaacctgttg cagtttttgc tgcgcattcc ccagcacatt
     4921 ggcaacggca tcaattgcgg acggacgccc caccaggttg aggatatcgc cgaactgcag
     4981 gctgatatcg ccactggcga ccagttcgac cccggcacgg ttcaggcgcg agataacaac
     5041 gtcatagcgt tctttaaagt gcaggtcgcg aatacgtttt ccgagcacgt tttcattggt
     5101 gaccaccaca cgctcgacgc gcaaatcagt gtctttcgtg gacagcgaag tatcgacctc
     5161 ctgaccaatc accagttgcg cattatgtaa atccgctggc tgacccacca gatgcagcaa
     5221 atcgcccagt tggataatgg tatctggcga aggaactttt agggtttctt cgcgtttcag
     5281 acgcgagcag ataattttgt cgccgttgag aatcggtaca tctttaatgg ctaaatcatg
     5341 caggttaggg ttctcaacgc gaatattgat agtcttgatc agcgcgccgc cattggtgcg
     5401 tgaagactcg tgctgctgag cttctgtctc gacattgacg cggaaaataa cccgcaacat
     5461 ccacatggtg aacaaaatcc cgcaaatgcc gaatggatac gccatcgcgt aactcatccc
     5521 catctgatcg accatttcca ttggtgtacc caggtcgcgc aaaatctgct gccctgcccc
     5581 cagcgctggc gtattggtaa ccgcaccgga gaaaatcccc agcactaccg gcagtggaat
     5641 atcaaacagt ttatgcagga tggcggtaac cagaccaccg atgatgacga tcagaacagc
     5701 aaacaggttg aggcgtaatc cggagacgcg caatgaggca aagaagcccg gccctacctg
     5761 aatcccgatg gtataaacga acaggatcag gccaaattcc tgaataacat gcagcatatc
     5821 gctacttaat gtcatccccg cctgagaaac aaaatggccg acgatgatcc caccaaacag
     5881 cacgccgcca atacctaatc ctatgccgcg aaatttgacg ttgccgataa acaaaccgac
     5941 gactgccacc aaagccagaa tactgaccgt taatgctata tcactcatcg tccatttctc
     6001 ttgagataac attttaattg ctacggatta tggctgaatc ggtgaagcag atttgcctta
     6061 tagcgcacat tattacggga aagaacgtgc cgaaatatct taaacagtcg aaatttaacg
     6121 cattgttgaa gaagaaagac aaaaaaagcc ccaccagaat ggcggggcaa agagaatagc
     6181 tagttagcta tttaacgcgg gacgttcgct gatagcgata cgctgcgctg cgatgggttc
     6241 aggctcatta cgaattaaat caatatgcag taaaccgttt acgaaagttg cgccagagac
     6301 ttccatattt tcagccagcg taaagctcag gctaaatggc tgattcataa gcccttgatg
     6361 cagccatttt ttctcttctt ttggctgctc cggcgtgcct tttacgctca ggcgcgtacc
     6421 ttccagttga atctctaaat cttcctgacg gaaacctgcc agcgcaaggg taatgcggta
     6481 gtggttatcg tcgcttttct caatgttgta cggcgggaag ctctgacttt caccggcgtt
     6541 ttgcagtgcg ttggccagtt tgtcaaaacc gatccattga cgcatcagtg gggataaatc
     6601 gaagttacgc atagtcattt ctccttctaa gaagcgagta agtacctgca aatccgaaga
     6661 ttcgcatatg ctccctgacg gcgagcatgg agatgtcagg ccgcgccagg cggccttaag
     6721 gaattagttg atttcgatac ggcgcggttt tttcgcttcc ggaatcacgc gttcgagatc
     6781 gatatacagc aaaccattta ccaggttagc gccacgaaca tgaatgttct cagctaactg
     6841 gaatttgcgt tcaaagttgc gttcagcgat gccctggtac agataggtgc gctctttttg
     6901 ttcgtcggcg tgagcacctt tcaccaccag cagattatcc tgggcggtaa tttccagttc
     6961 gctctcagca aaaccagcca cagcgatagc aatgcggtaa tggttttcgt ctaccagttc
     7021 aacgttatac ggagggtagc cgccattact ctggctctgg ttgttttcta agtggttaaa
     7081 caaacggtca aatccaatag cagaacggta aagcggggat aaatcaaagt tacgcataat
     7141 caatagctcc tgaaatcagc gagaatgtaa gaccttccac aatggacagg tcaggtagcc
     7201 agaacaccca tcaggcgcgt cctcatcgac tacgatgtaa aaatgggtct ggaaatgaac
     7261 ttttcaagcc ttatcagcgg actttttttt gcagtttatg gtctattgca tagactgagg
     7321 gggcagcacg aaacgttaaa gtgcgatagc cgccacagag cgacgtaatg atgatgttat
     7381 tttcagcaag ggatcgctat aactcatcag gccaacaccc acgatcaaca gatgaattta
     7441 ttatgataag aaatgtgttg ttagcgttca tgatatgcag cggaatggca ttactcggag
     7501 gatgctccag cgtgatgtcc cataccggag ggaaagaagg aacgtatccc ggcacccgcg
     7561 ccagcgccac catgattggc gatgatgaaa ccaactgggg cactaagtcg ctggcgattc
     7621 tcgatatgcc gtttaccgct gtgatggaca cgcttttatt accgtgggat gtgttccgca
     7681 aagacagctc ggtgcgctca cgcgttgaaa aaagcgaagc caacgcgcaa gccaccaatg
     7741 cggttattcc accggccaga atgcctgata actgatttag cgatcagttt cggtgatcca
     7801 cagctggcca ccttccatcc acgccagcca ttgaccatcc ggcgagaaga ccacggcgtc
     7861 cgcagaaggt ggattggcgt ggttttcggt taaatactca acctcgccgc tttgcgcatg
     7921 ggcacaagca attcgattat ccagcacaaa gcccaaccat tctcctgacg gatgccagtt
     7981 aaatgcagac tgaatatccg ttttgttatg ggttaactgg cgcggctcgc cgccctgtgg
     8041 cgagataagc cacagttgca caatgccgtt atcatcacgc attaaaaacg cgatttgcgt
     8101 accctgcgga ttacagcgca cccagtggcg cgggacgttg actaaccccg gataagcccg
     8161 atggtgggta aaggttaaac gtcgctgcac gacgccacgc ggtggcgcgg gcagggtggt
     8221 ttccgttcca cttaacggcg catcacctgc cgctttccag ccagcttcat cttgcggcaa
     8281 ctcaacgata aacagctccg gcactttctc accctttggc gaaagtgtgt cgccaataaa
     8341 cgccagcgcg tgatttccta cccatccttc ttcataagca cgattgattt catcactgcc
     8401 aggctgcggc gtgggcgtgg ttttactcac cagcacacac cagtggctgc cgctgtattc
     8461 acgcggatgc tgcttttgta cgttgaccgg accaaacggc gcagcaacac cgacgtttcg
     8521 caaatccagc gccggatcga gttcatgcat tacatggtcg ttataggtaa agctcaccct
     8581 ttcaccgttc gggctaaaga catgcacatg gctgccgccg cgcagcgctc ctggcgtata
     8641 cggcgcggta atatccattg catcgagatt gctcaccttg ccgccttcag caatgactcc
     8701 gcgccgatga tggaagtcgt aatgccatgt ttcatcagga ttttccgggc cgtgaataaa
     8761 gacatatttc tctgacttcg gatgaacggt caccacgccg acgtgtgcgc cctgtgacgc
     8821 acagtagata acctcgacct caccggtatg gatattcaca cgctcaatgg tctcgccggt
     8881 aaacgacgcc ccagaaggac gcacgtcaaa taccagccac tggctgtcgg gcgtccaggt
     8941 attggtattg gtgagcaggt gattacgggg agcaaaggtt atctgtttca tggcgcggtc
     9001 ctgatacaaa acgggtccag ccatcatact tcacaaggac ttcactttca gacaatagtc
     9061 ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat ttcgacgtgg cgattattgg
     9121 cctcggcccg gcagggtcgg cgttggcacg aaagttagcc ggcaaaatgc aggtgatcgc
     9181 gctggataaa aagcaccagc atggtactga aggtttcagc aaaccttgtg gcggtctgct
     9241 ggcaccggac gcgcagcgat cttttattcg cgatgggctg acgcttcctg tcgatgtgat
     9301 cgctaatccg cagattttca gcgtcaaaac cgtcgacgtc gccgcatcgc tcacgcgtaa
     9361 ctaccagcga agctatatca atattaatcg ccatgctttc gacttgtgga tgaaatcgct
     9421 gatccccgcc agcgttgagg tttatcacga cagcctgtgt cgaaaaatct ggcgtgagga
     9481 tgataaatgg catgtcattt ttcgtgcaga cggctgggag cagcatatta ccgcccgcta
     9541 tctggtcggt gccgatggtg ccaactcgat ggtgcggcga catctctacc cggatcatca
     9601 aatccgtaaa tatgtcgcaa tccagcagtg gttcgcagag aaacatccgg tgccgttcta
     9661 ctcctgcatc tttgataatg cgataactga ctgttattca tggagtatca gcaaagacgg
     9721 ttattttatc tttggcggtg cctatccaat gaaagacggt cagacgcgtt tcacgactct
     9781 gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa gcggtgaaaa gcgaaaaatg
     9841 cacggtgctg tttccctcac gctggcagga ttttgtctgc ggtaaggaca acgcctttct
     9901 gattggtgaa gcggcgggat ttatcagcgc cagctcgctg gaggggatta gctatgcgct
     9961 ggatagcgca gacattctgc gttcggtgtt actgaagcag ccagagaagc tcaataccgc
    10021 ttactggcgc gccacccgca aactgcgttt aaaactcttc ggcaagatag taaaaagccg
    10081 atgcctgacc gcaccggctt taagaaagtg gattatgcgc agtggtgtgg cacatattcc
    10141 acagttgaag gattagccaa cgcgcttcac atcgcccacc agcaggatat aagagagcgc
    10201 gccaatcaac gcgacggcgg agatataaac cagtgcaggt gcgaaaccgt aaccctgcgc
    10261 caggtacccc accaccagcg gaacggtgat gccgcccaga ccaccggcga agttaaacac
    10321 gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa gaaaccagcg accaggtaat
    10381 cgaagcaaaa ccgttaccga agaatgccag cgccatcagg cacataatca tcatcggatc
    10441 gttagtgtag ttagcgccca taatgcaggt ggagatcagc aagccgcaga taatcggcgt
    10501 tttacgcgca aagcccagtg aaaagccctt acgtaccagc agatccgcta cccagccaga
    10561 gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg gtcataaagc ctgctttcag
    10621 cgccgtgatt cctttttcct gggttaaata gttcgggaac caggttaaga aaaaccacag
    10681 tgtagaagcc accgcaaatt gcccaagata gacgccgatc agtttacgat ggaacaccag
    10741 tttccagtct ttggctgtta acggctgacg tgcctctttc ttcactggcg catcaccatc
    10801 caccagaccg ccgccatcac gaatgtaatc cagttcagct ttgctgatgc ctttggtcag
    10861 gcgcggcggc tgataaacct taaaccaaat cagcgaccag ataatgccga taccaccagt
    10921 cacaatgaac acccagtgcc agctcaacat ctcctgaatc cagatcagca gcggagtcaa
    10981 aaacgccaga ccgacaaact gaccagacgt ataaaaacca acggcggaag cgcgttcatg
    11041 ttccgggaac cagctggtca ccatccggtt attggtcggg aaagcgggcg cttcgaaaat
    11101 accggttatc gcacgcagac caattaatga cattaatccc gtggcaaagc cctggaataa
    11161 agtcgccact gaccagccaa atatcgcaat aaaataagtc acgcgagaac ctacgcgatc
    11221 taaaaaccaa ccgccgggga tctggcatag cgtataaagc caggcgaagg ccgaaaatac
    11281 atagcccatt tccgctttcg taatgccgaa ctcttcctga atatgggcgg aagccacggc
    11341 caggttagcg cggtcaacat aacaaatgac caccgtaata aagatcatca ccagcgtcag
    11401 ataacgccga cgccccggct ttgctgcatt aacgggaata tccatagcga gctttctcca
    11461 gattttgggc atagcgaagc cgctcaccat gccctgtaat ttacagaggg ttatattttt
    11521 gtattgctgt tttagtgccc gatgaggggc ttacgtggca ggaattacca ctctgctacg
    11581 ctgttatctt catgacgcca gagtggatta cgccagtccg gggcattttt actgaactca
    11641 ataactttag cttcgtcgat ttccacgcct aagcccggtt tcgttagcgg tttaaagaag
    11701 ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt cgagtaactc cgcgcctttg
    11761 ttgtaatgaa ttcccatact ttgttcctga agtaccgcgt tataggaaac aaagtcgata
    11821 tgcagacaag ccgccagtgc aatcggtccg agcggacagt gcggcgcaag ggtcacgtca
    11881 taggcttctg ccattccggc aattttgtag cattcggtaa taccgcctgc atgggagaga
    11941 tccggttgta gaatcgaaat accgcctgcc tccagcacgc gtttaaaatc gaaacgtgag
    12001 aacatgcgct cacccgccgc cagtggaata tgcgtttgtg ccgccaattt cgggtagtat
    12061 tcggcttgtt ccgccagcac cggctcttca ataaacagcg ggcgatacgg ctccagttct
    12121 ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt ggaaatcaag accaaactca
    12181 atctgattgc caaaagcttc acgaatttgt gccacggtgt tgactgccgc atctaccgcg
    12241 cgggagttat caattagccc cagttcttca caaccgttca gtttgaaggt atcgaagccg
    12301 atttcgcgca gggttttaat accgtcgatc acatccgccg gacgatcgcc gccgacccaa
    12361 ctgtaggctt taattttgtc gcgaaccagg ccgcccatca gttgccagac cgacgcattc
    12421 agcactttac ctttgatatc ccataacgcc tggtcgatcc cggcaatggc gctcatcagg
    12481 attggaccgc cacgataaaa tccggcgcga tacatcactt gccataagtc attgatgcgc
    12541 tatggatcct gaccaatcaa atagtcaccc agctcgtgaa ctgccgcttc caccgtgcgg
    12601 gcgcggcctt caatcactgg ctcgccccag ccgacgacgc cttcatcggt ttcaattttc
    12661 aggaacatcc agcggggagg taaacgatac gtggtaattt tggtgatttt cattgcactg
    12721 cctctcgata cgccttaaca aatgctgctg cctgctgcgc ggtgcgttct acggactgcc
    12781 cggcgcgata gagatcgctg cctaagcccg ccccagcaca acctgcgtct atccactgcg
    12841 ccaggttttc tggcgtcacg ccgccaacgg caaagactgc gatgtccgat ggcaataccg
    12901 cttttaacgc tttgatgtat tgcggaccaa aagccgatga cggaaatatt ttcagcgcct
    12961 gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt cgcgcagccg gggcagacgg
    13021 tcatgccgta gcccaccgca cggcggatca cttcactatg gatattgggc gtaacgatga
    13081 gctgacagcc catcctggcg agcgcatcga cctgttcagg tttcagtacc gtacctgcgc
    13141 caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg aatgctttgc tcccattgtg
    13201 gggaattcag cgggatttca accgcgtcga acccggcgtc aatcaccgcg ccaacatgcg
    13261 ccagcgcctc gtcgggctta atgccgcgca aaatggcgat cagcggaagt ttagtttgcc
    13321 actgcatgag cgatgctcct tataccagcc tgaaatgccg tgtcgcccgc caccgccgtc
    13381 acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg cggtcaacga tgttccggcg
    13441 acaagggtga tggcgtgttg atgggccaca tagtcgcgca tactggcgac ctctgcgcca
    13501 atcaacaaac cagagagaaa ttcactgacc tgttcacggg gaagtgttcc cagcacatgc
    13561 gaggcacgaa cttcaaaaag ctgcggcaat atggcgggcg tattaagacc acgctcaagg
    13621 ccagccgtga aggcatcggc agagttttcc tgcggcggca aacctgcacc aatcaatgag
    13681 tgatttaaca gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc gttgatttgc
    13741 tggctatcgg cctgcaccca tttgcaatgc gtaccgggca tgacataaag agaggaagga
    13801 gccagagctc gcgcgccgat caattgagtt tcttcgccgc gcatcacatt gtggttatca
    13861 tcatgagaga cgcataatcc gggaataatc cagatattgt cgccaacaga cgttaattgt
    13921 tcgccaatag acgaaaaacg ggcaggaaca gataaatacg gagcaacttt ccagccgaca
    13981 ttgctgccga ccattcctgc cattaccact ggcgtattct cttcacgcca gtcggtcgtg
    14041 acttctgcta acaccgcagc cggagatttt ccgttcaggc gcgtgacgcc tgcttctgat
    14101 tgcctgctct ccaggcagtg gtcgccctga taaagccagg cgcgcagatt ggtcgatccc
    14161 cagtcaattg cgatgtagcg agctgtcatg tgatttcctt taaccttcgt gtcgagctgg
    14221 cgatcatggt aagcgccgcc tgctctgccg catcgccgtc ctgatgccgt atcgcatcga
    14281 acagcgcctt atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc atccaggttc
    14341 gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct aagttgctgt aacaccgggt
    14401 tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc
    14461 ggtcctgatt gttggcaatc atctcgttca gcgccgattc aatctgcgcc agatcgctgg
    14521 aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc caccagattt cgcacttcgc
    14581 tcatggcact gataagccgt gggtcgtagt cgttttccag cacccattgc agtacgtcag
    14641 tgtcgaggta attccactgg ttacgcggtg ccacaaacgc gccgcgataa cgtttcattt
    14701 caatcagccg tttcgccatc aacgaacgga acacctcacg gatgatgttg cgcgaggttg
    14761 caaactcctc acagagttcc gcctcagccg gaagcggcga gcctggtacg tatttgccgt
    14821 gaacgatctg tttacccagc gtaatgacga tgcgatcggt tttattgaga gtcatggaga
    14881 gtccttgtgc tctgtatgtt cttctctact ttaccccgat cgatgcataa cacggcaact
    14941 ttgtagtacc agcgtgatga cgttcgcgtt tgctgtgcgt gtaatgtagt acaaacttat
    15001 attgttgtac tacaatttag atcacaaaaa gaacaatgcg taaaaaaatg acatgcgtcg
    15061 ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag ggaaagaaga gtatgctttc
    15121 aacggcttag ctactcgttt aaaggattaa tcatgaagtt gaatcttaag ggatttttta
    15181 agactgccgg tttattccca ctggcgctga tgctttcggg ctgtatctcg tatgccctgg
    15241 tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac accatcacgg
    15301 ggctatcgca ggcaaaggat agtaatggaa caaaaggcta tgtttttgta ggggaatcgc
    15361 tggattacct tatcacagat ggtgccgatg acatcgttaa gatgctcaag gatccagctc
    15421 ttaaccggca caatattcag gttgctgatg acgcaagatt tgttttaaat gcggggaaaa
    15481 agaaatttac cggcacaata tcactctatt acaactggag cacagaagaa gaaaaagcac
    15541 gagtgacaca atatcgtttt gcttgtggtg ttcagcactg tacccggaca gtagaaaacc
    15601 tggaaggtac tatccatgag aaaaataaaa acatggatta ctcaaacgta atggctttct
    15661 accatccatt taaagtgcga ttttatgaat accattcacc cagaggcatt ccggatggtg
    15721 tttccgcagc attactgcca gtgactgtta cgctggacat cattactgca ccgctgcaat
    15781 ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt
    15841 tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac gccatcttca
    15901 aggttagatt tggtgacaaa gttcgccact tctttcaccg aaggaatagc gttatccatc
    15961 gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc
    16021 atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc cgtacctttg
    16081 ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac ggtatatttc
    16141 tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg ttcatcaatc
    16201 atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca gaacaccagc
    16261 ggaatagtgg caacgaaaga ttcatgcact gtgtagtagc tgatatcgcg gttggcggtg
    16321 tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc
    16381 aggaaacgat agtcgtcata gctgagagca gtttgcgcca cggtgctacc atcagcggcc
    16441 ttctgtacta gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc catatgcagc
    16501 tctttcaggt agttgtgcac acctgcatac gggcgacccg tcgttagcac gacattcacg
    16561 ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc
    16621 agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag ttccccaggt
    16681 agattggttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca acacccgagg
    16741 gaaaaggggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa gagtttccct
    16801 ttcagcagtt ccatacctgc ggaaagcaga tcgttattgg cttgtggtga cacttcacct
    16861 tgcggtgaga gcgcatcaat aatcttcggc aattgctctg ccagtaaact ggaagctgta
    16921 ctggtatcca cgccaagttt ttgcccgaga tcggacaccg catttgtgcc gagcgccgat
    16981 tccagttgct cgccactaac cgattgattg ccctgttgat tactcagcca ggttgagaga
    17041 atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc gccctgctcc
    17101 tcaacccaac ttaaaatagc ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca
    17161 acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt
    17221 atagatttgc gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg
    17281 cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc
    17341 atgaaaattc aatacattgc aagattttcg taggcctgat aagcgtagcg catcaggcac
    17401 gctcgcatgg ttagcgccat taaatatcga tattcgccgc tttcagggcg ttctcttcaa
    17461 taaacgcacg gcgcggttca acggcgtcgc ccatcagcgt ggtgaacaac tggtcggcag
    17521 caatcgcatc tttaacggta acgcgcagca tacgacggct ttccgggtcc atggtggttt
    17581 cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg gagaggccgc
    17641 gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc ggctgacgac
    17701 gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc tcacccagcg
    17761 tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga tagtcagtat
    17821 ccacaccgtg ggtacgcacg cgaacaatcg gttcaaacag gttttgttcg gcattggtgt
    17881 ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg
    17941 cgttcaccca gcgggtaacg gtctgctcat cagagaggtc ggcttccgtc agcgtcggct
    18001 gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg ttgatcattt
    18061 tctgcgtcgc gttgtactca gacaccaatt tctctaacgc ttcgccagcc aatgccggtg
    18121 cgctggcgtt ggtgtgcagc gttgcgccat ccagcgcgat agagatctgg tactgatcca
    18181 tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg tacagcggcg
    18241 gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga tagaagaagg
    18301 tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc
    18361 tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacag ccaagcgcgg
    18421 tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct ttctcaacgt
    18481 tgaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc ccttgcttcg
    18541 cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt
    18601 cctggcagtc tgccagtttg cccggcaggc ccgctaagtc gagcgcacct ttacggcggg
    18661 tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata attttgccaa
    18721 ccacgatttt cgcgtcggtt gggttttcca gcaggtattc tgccagcagt tcgttcatct
    18781 gctgttcaac cgctgatttc acctcagaag aaaccagttt gtctttggtc tgggaggaga
    18841 atttcgggtc cggcactttc acggaaacga ccgcaatcag gccttcacgc gcatcgtcac
    18901 cggtggcgct aactttggct tttttgctgt agccttcttt gtccatgtag gcgttcaggg
    18961 tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt
    19021 tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcaac gccacttcga
    19081 cgccaatacc gtctttttca gtggagaagt agaagatatt cgggtggatc ggcgttttgt
    19141 tcttgttcag atattcaacg aacgccttga tgccgccttc atagtggaag tggtcttctt
    19201 tgccgtcgcg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac gacaactcac
    19261 gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag gtttcgaggc
    19321 tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc gccaacggtg
    19381 cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc tggataacca
    19441 gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg
    19501 acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc atgatcactt
    19561 ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct
    19621 gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag tgacccgcga
    19681 gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga ccggtgccgt
    19741 catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc cctttcagga
    19801 ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca tttatacttg
    19861 ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc gtccgacatg
    19921 tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt cgcttttaag
    19981 cggctggcaa gcagcccgcg acgctcatca tcaagctcag aggcaaaatc atctatcagg
    20041 tagagacacc gccgcccgct ttcacgggtg aggaactctc cttgcgccag acgtaaggcg
    20101 cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc accgtcggcg
    20161 cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt
    20221 tcaaaattac gttccagcac ctcagcatat tctgtctctt tctcccagcc gcgctggaaa
    20281 gagaaagtca gagaaaactc agggagaaat tgcttacaag tatcggccat atcagccgcg
    20341 ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg gatcagctct
    20401 ttatcccacg ggcgtagctg ttcgtaacgt gtcacctggc gcagcgccgc attgcgctgc
    20461 ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc cgggttcgtt gtgaaagcat
    20521 ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt aaacccttct
    20581 ggcgttatca actgcattgg catcaggtgc gccagttccg cgaccttatg cccgtctgta
    20641 ccgtcgatgc ggactttgct gtcgccctgt ttgtctttgg ttaagccaat cgctgtctcg
    20701 cgctcttcgc cctgtaatcg cccgtggaga acaaacgcct cctgctcatg gcgaatgacg
    20761 cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat ggcttccagc
    20821 acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg agataaagcg
    20881 agatccgcgg tttcaatgtt gcggaaatcg cggatcaaca agcgggtgag ggacattaca
    20941 gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca atctgcacgc
    21001 tggaaaccga atcggtcagc atcatgcgga cgttttcaca tttcagcgcg ttcagaacat
    21061 ccagcacgta gctgacgttg aagccgattt ccatctccgc accgctatag gtaacgtcga
    21121 ggatctcttc cgcttcttcc tgttccgggt tgttggcggt gattttcagc tggttttcgc
    21181 tgacataaag acgtacgccg cggaatttct cgttagagag aatcgccgcg cgagcaaacg
    21241 cctgcttgag cagatcgcag ccagcttcca gatgtttgtc cgggttcttc ggcagaacgc
    21301 ggcgataatc cgggaagcga ccatccacca gtttggaggt gaagataaag tcgccaacgt
    21361 gggcgcgaat gttgttgctg ccaatctgta cgcgcagcgg attgtcgccg ccgtcgagca
    21421 tacgcatcag ttcaatcacg cctttacgcg gcacgatcac cgaatggctt ggcaaagatt
    21481 gaccaattgg cattgaacag actgccagac ggtggccgtc ggtcgccacg gtgcgcagtt
    21541 cttcgccttc ggtttcaaac agcataccgt ttaagtaata gcgaacgtcc tgatgcgcca
    21601 tagaaaactg ggtcgcttca atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga
    21661 cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta gacagcgaaa
    21721 aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgtacg gcaatttccg
    21781 cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc
    21841 ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg agatcagtac
    21901 cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga atcggtagcg
    21961 taggacgacc acctaacggc ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt
    22021 ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga aaaatcttct
    22081 ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc
    22141 gtgtggtcac gaccaccaaa cgcatcgcca atctccggca gactgtggtt agtcagctct
    22201 ttcgccagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa
    22261 aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat attgtcgatg
    22321 gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagtg cctcacgcac gaagtcgatg
    22381 gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc cccttccagc
    22441 tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcgcc tggcaaacga
    22501 atgtcatttt cgtcggcctt tttcatcagg atcgccacgc gggtttccag ctctggcggt
    22561 tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc aacgccgttg
    22621 atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc cagcagggcg
    22681 ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg
    22741 tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat cgcgttgttt
    22801 tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac cactttggca
    22861 ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt tttacccaga
    22921 cccgtgccgc cataaaggaa caatgggtta taggcaccgc cagggttatc cgccacctgg
    22981 cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt
    23041 ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt atcccaacct
    23101 gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg
    23161 acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa
    23221 cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat attattaagg
    23281 tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc cagcgtgtta
    23341 tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc tggtaactca
    23401 tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga ctccactcga
    23461 acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac gtcgaaaaga
    23521 ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat caagatcctg
    23581 caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc tgtttctcac
    23641 agattttccc gatttatcca caggactttc cagacctcgc taagtgtaaa cgatcctgcc
    23701 gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag acaaaaattg
    23761 gcttaatcga tctaataaag atctaggacg atccttgcgc tttacccatc agcccgtata
    23821 atcctccacc cggcgcgcca tgctggtttt cactggtgtg aggtcgtaca ttttccctgc
    23881 gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccgga gtgtacaatt
    23941 attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc atttttcggc
    24001 ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc atgaaacgca
    24061 cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct cgtatggcta
    24121 ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg
    24181 tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag ggagttacgc
    24241 ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc tggcacgccg
    24301 caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg tcttacagtc
    24361 gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa
    24421 agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa
    24481 ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc
    24541 cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa cgcctgatta
    24601 gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac ggaattgagg
    24661 cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc gtattaaaat
    24721 gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt gataccagag
    24781 aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt
    24841 catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc aacagaccac
    24901 gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca
    24961 ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca cccgtggtgg
    25021 tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta cccagccgtt
    25081 ccagctgctg gaaacttcac cgcagtttat ttatcaggca cagagcggtc tgaccggtcg
    25141 tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg aaaaagacgc
    25201 ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata ccgacgcggc
    25261 aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa
    25321 ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tccacctttg gtcagttgaa
    25381 gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg cactgcacac
    25441 cttccgcggc gcggcgtact ccacgcctga cgagaagtac gagaaataca agttcgatac
    25501 cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg caatgctgca
    25561 acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact tctataccgc
    25621 taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac tggttcagcc
    25681 tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc aggacaaaat
    25741 ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt ggttcatctc
    25801 tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact ggggcttctc
    25861 cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca aagcgcagta
    25921 cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc gtgagcgtct
    25981 gggcgatgac aaacagcgta tcagccagga aatgatggcg ctgtacaaag ctgagaaggt
    26041 taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc tggcgttgta
    26101 ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt ggatccacga
    26161 cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa cgatgttctt
    26221 cattcagaag atgtcgccga ccacagtgac cgacccgatg cagcagaaga tcatgacctt
    26281 tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg tgctgtacta
    26341 tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg gtctggaaaa
    26401 acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt cgctaaaata
    26461 agggcggtca gttgaccgcc ttttttcttt tcgtagggtg gataagcacc gcgcatccgc
    26521 cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc aggccacgcc
    26581 tccgggacgt ggcggcgttg gcatcctgcg catctccggc ctaaaagccc gtgaagttgc
    26641 cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga
    26701 cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc cgaactcgtt
    26761 caccggcgaa gatgtgctgg aactgcaggg tcatggcggt ccggtgatcc tcgacctgct
    26821 gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg agttttccga
    26881 acgcgcgttt cttaacgata aactcgactt agcccaggcc gaggcgattg ccgatcttat
    26941 cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag gcgcattctc
    27001 cgcacgggtc aaccatctgg tggaagccct cacccacttg cgcatttacg tcgaagcggc
    27061 aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg aagcccagct
    27121 taatgacgtg attgccgatc tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt
    27181 gcgcgaaggg atgaaggtgg tgattgccgg acgtcctaac gccggtaaat cgagcctgtt
    27241 aaacgcgctg gcgggccgtg aagcagcaat cgtaactgat atcgccggaa ccacacgtga
    27301 cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg ataccgctgg
    27361 gctacgtgaa gccagtgacg aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat
    27421 tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acaacagacg ccgtggatcc
    27481 ggcagagatc tggccggaat ttatcgcccg tctgccagcg aaactaccga tcaccgtggt
    27541 gcgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga acggtcacgc
    27601 gttaattcgt ctctcggcaa ggacaggtga aggcgtggag gtgctgcgta accatctcaa
    27661 acagagcatg ggctttgaca ccaacatgga aggcggcttc ctggcgcgtc gtcgccacct
    27721 acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac tgttaggtgc
    27781 ctgggcgggt gaactgctgg cggaagagtt gcgcctggca cagcagaact taagcgaaat
    27841 caccggggaa tttacctctg atgatttgct ggggcggatt ttctccagct tctgtattgg
    27901 taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg cttcattgtt
    27961 agcactcctg ttattcctca accctttttt taaacattaa aattcttacg taatttataa
    28021 tctttaaaaa aagcatttaa tattgctccc
//
LOCUS       DAZRUF010000025        28043 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZRUF010000025 DAZRUF010000000
VERSION     DAZRUF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28043
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.025"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..834
                     /locus_tag="ACHXOL_000894"
     CDS             <1..834
                     /locus_tag="ACHXOL_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR6807946.1"
                     /translation="ILNRRDIVLESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDK
                     FIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYSGIKDYPVCFYD
                     PKYKIMANHHGITFDDQRKRYIIGPITVSSDEMTNALDPVELAAIINKKHHAVINGKK
                     FKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRECIGETGT
                     VLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQ
                     L"
     gene            1024..1137
                     /gene="yhiY"
                     /locus_tag="ACHXOL_000895"
     CDS             1024..1137
                     /gene="yhiY"
                     /locus_tag="ACHXOL_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR6807947.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1200..2267
                     /locus_tag="ACHXOL_000896"
     CDS             1200..2267
                     /locus_tag="ACHXOL_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR6807948.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2264..4999
                     /gene="rbbA"
                     /locus_tag="ACHXOL_000897"
     CDS             2264..4999
                     /gene="rbbA"
                     /locus_tag="ACHXOL_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR6807949.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4999..6123
                     /locus_tag="ACHXOL_000898"
     CDS             4999..6123
                     /locus_tag="ACHXOL_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR6807950.1"
                     /translation="MRHLRNIFNLGIKELHSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6196..6469
                     /locus_tag="ACHXOL_000899"
                     /pseudo
     CDS             6196..6469
                     /locus_tag="ACHXOL_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6466..6825
                     /locus_tag="ACHXOL_000900"
     CDS             6466..6825
                     /locus_tag="ACHXOL_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR6807951.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6945..7346)
                     /gene="nikR"
                     /locus_tag="ACHXOL_000901"
     CDS             complement(6945..7346)
                     /gene="nikR"
                     /locus_tag="ACHXOL_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR6807952.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7352..8158)
                     /gene="nikE"
                     /locus_tag="ACHXOL_000902"
     CDS             complement(7352..8158)
                     /gene="nikE"
                     /locus_tag="ACHXOL_000902"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR6807953.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(8155..8919)
                     /gene="nikD"
                     /locus_tag="ACHXOL_000903"
     CDS             complement(8155..8919)
                     /gene="nikD"
                     /locus_tag="ACHXOL_000903"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR6807954.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(8919..9752)
                     /gene="nikC"
                     /locus_tag="ACHXOL_000904"
     CDS             complement(8919..9752)
                     /gene="nikC"
                     /locus_tag="ACHXOL_000904"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR6807955.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9749..10693)
                     /gene="nikB"
                     /locus_tag="ACHXOL_000905"
     CDS             complement(9749..10693)
                     /gene="nikB"
                     /locus_tag="ACHXOL_000905"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR6807956.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10693..12267)
                     /gene="nikA"
                     /locus_tag="ACHXOL_000906"
     CDS             complement(10693..12267)
                     /gene="nikA"
                     /locus_tag="ACHXOL_000906"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR6807957.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12378..12965)
                     /gene="acpT"
                     /locus_tag="ACHXOL_000907"
     CDS             complement(12378..12965)
                     /gene="acpT"
                     /locus_tag="ACHXOL_000907"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR6807958.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13020..14069)
                     /locus_tag="ACHXOL_000908"
     CDS             complement(13020..14069)
                     /locus_tag="ACHXOL_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR6807959.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14201..15418
                     /locus_tag="ACHXOL_000909"
     CDS             14201..15418
                     /locus_tag="ACHXOL_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6807960.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMLWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15422..15979)
                     /gene="dcrB"
                     /locus_tag="ACHXOL_000910"
     CDS             complement(15422..15979)
                     /gene="dcrB"
                     /locus_tag="ACHXOL_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR6807961.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16052..16717)
                     /locus_tag="ACHXOL_000911"
     CDS             complement(16052..16717)
                     /locus_tag="ACHXOL_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR6807962.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            16938..17183
                     /gene="tusA"
                     /locus_tag="ACHXOL_000912"
     CDS             16938..17183
                     /gene="tusA"
                     /locus_tag="ACHXOL_000912"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR6807963.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17285..19483)
                     /gene="zntA"
                     /locus_tag="ACHXOL_000913"
     CDS             complement(17285..19483)
                     /gene="zntA"
                     /locus_tag="ACHXOL_000913"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR6807964.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19557..20183)
                     /locus_tag="ACHXOL_000914"
     CDS             complement(19557..20183)
                     /locus_tag="ACHXOL_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR6807965.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            20324..20683
                     /locus_tag="ACHXOL_000915"
     CDS             20324..20683
                     /locus_tag="ACHXOL_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR6807966.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20686..20955)
                     /locus_tag="ACHXOL_000916"
     CDS             complement(20686..20955)
                     /locus_tag="ACHXOL_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR6807967.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20945..21541)
                     /gene="rsmD"
                     /locus_tag="ACHXOL_000917"
     CDS             complement(20945..21541)
                     /gene="rsmD"
                     /locus_tag="ACHXOL_000917"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR6807968.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21691..23187
                     /gene="ftsY"
                     /locus_tag="ACHXOL_000918"
     CDS             21691..23187
                     /gene="ftsY"
                     /locus_tag="ACHXOL_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR6807969.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23190..23858
                     /gene="ftsE"
                     /locus_tag="ACHXOL_000919"
     CDS             23190..23858
                     /gene="ftsE"
                     /locus_tag="ACHXOL_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR6807970.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            23851..24909
                     /gene="ftsX"
                     /locus_tag="ACHXOL_000920"
     CDS             23851..24909
                     /gene="ftsX"
                     /locus_tag="ACHXOL_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR6807971.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25154..26008
                     /gene="rpoH"
                     /locus_tag="ACHXOL_000921"
     CDS             25154..26008
                     /gene="rpoH"
                     /locus_tag="ACHXOL_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR6807972.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26279..27382
                     /gene="livJ"
                     /locus_tag="ACHXOL_000922"
     CDS             26279..27382
                     /gene="livJ"
                     /locus_tag="ACHXOL_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR6807973.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27581..27964)
                     /gene="panM"
                     /locus_tag="ACHXOL_000923"
     CDS             complement(27581..27964)
                     /gene="panM"
                     /locus_tag="ACHXOL_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR6807974.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWIADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6656 a         7284 c         7773 g         6330 t
ORIGIN      
        1 atcctgaaca gaagagacat tgttttagaa agtcttcagc cttatttggt tattaataaa
       61 gacgcagtca cgccatgtac gtttctgggc gaccagactg gcgatcgatt tagtaatatc
      121 tgtggcgata aatttatcat cgatttatta aaacgcataa tgagtatcaa tgaaaacgtt
      181 catgtattag ctggaaatca tgaaacgaat tgcaatggta actatatgca gaacttcacg
      241 cgcatgaaac cacttgatga ggatacctac tctgggataa aagattatcc tgtatgtttc
      301 tatgacccca aatacaaaat aatggcaaat catcatggga taacttttga tgatcagcga
      361 aaacgttaca ttataggacc gataacagtc tctagtgatg aaatgaccaa tgctctcgat
      421 ccggtcgaat tagcggcaat tatcaataaa aagcaccatg ctgtaattaa tggcaaaaag
      481 tttaaaaata gccgggctat ctcttgtcga tcctttaatc gttatttttc tgtttcgaca
      541 gattacagac ctaaacttga agcactatta gcatgctcac aaatgttagg tataaatcaa
      601 gttgttgcac ataacggtaa tggtggaaga gaatgtatag gtgaaacggg gacagtttta
      661 ggtcttaacg cccgtgacag taaacatgct ggaagaatgt tcagcatgca taattgccaa
      721 attaaccctg gtgcagggcc tgagataacc actccctgga agtcttacca gcatgaaaaa
      781 aacagaaatg gacttatgcc tttgatcaga aggcgcacaa tgttacagct ctgaaccata
      841 gacaacatga atattaatgt tactatctgg ttgagactgt tgataaaacg taaaaaggaa
      901 tgctctgttc ctgcaactga acaggcgtga aacgctatcc aacaggatgg ataccgtttt
      961 gtcagccagt tttatagatc acacttatga gtatgcgtgt ttcatttacg gccaggaatg
     1021 ctgatgatga caacattact acccgttttc actaagcctt ccccgttggc gctcaatgct
     1081 ctgcgcgctg gtcgtatttg tcgtttcctt cttatcccgg atggaagaat ccgctgaacg
     1141 tcgttcggcg gcagttgtaa cacgcgtcgt ttgcttgcag ggattatggc gggtcgatta
     1201 tggataagag taagcgccat ctggcgtggt gggttgtcgg gttactggtg gtggctgccg
     1261 tcgtggcgtg gtggctgttg cgcccggcgg gtgtgccgga aggctttgct gtcagcaatg
     1321 ggcgcattga agcgacggaa gtggatatcg ccagcaaaat tgccgggcgt atcgacacca
     1381 ttctggtgaa agaaggccag tttgttcgcg aaggcgaagt gctggcgaag atggatactc
     1441 gcgtgttgca ggagcagcga ctggaagcca tcgcgcaaat caaagaagca caaagtgccg
     1501 ttgctgccgc gcaggctttg ctggagcaac ggcaaagcga aactcgtgcc gcgcagtcgc
     1561 tggttaatca acgccagtcc gaactggact ccgtggctaa acgtcatacg cgttcccgct
     1621 cgctcgccca acgaggggct atttctgcgc aacagctgga tgacgatcgc gccgccgctg
     1681 agagcgcccg agctgcgctg gaatcggcaa aagctcaggt atcggcttct aaagcggcta
     1741 tagaagcggc ccgtaccaat atcattcagg cccaaactcg cgtcgaagcc gcgcaagcca
     1801 ctgaacggcg tattgccgca gatatcgatg acagtgaact gaaagccccg cgtgacggac
     1861 gcgtgcagta tcgggttgct gagccagggg aagtgctggc ggcaggcggt cgggtgctga
     1921 atatggtcga tctcagcgac gtctatatga ctttcttcct gccaaccgaa caggcgggca
     1981 cgctgaaact tggcggtgaa gcccggctga tcctcgatgc cgcgccagat ctgcgtattc
     2041 cggcgaccat cagttttgtc gccagtgtcg cccagttcac gccaaaaacc gtcgaaacca
     2101 gcgatgaacg gctgaaactg atgttccgcg tcaaagcgcg tatcccaccg gaattactcc
     2161 agcagcatct ggaatatgtc aaaaccggtt taccgggcgt agcgtgggtg cgggtgaatg
     2221 aagaacttcc gtggcctgac gacctcgtgg tgaggttgcc gcaatgacgc atctggaact
     2281 ggttcccgtc ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg gaaaaaacgt
     2341 tgcgctgaac aatattactc tcgatattcc ggcccgctgt atggtcgggc tgattggtcc
     2401 ggatggcgtc gggaagtcga gcttattgtc gttgatttcc ggtgcccgcg tcattgagca
     2461 gggcaatgtg atggtgctgg gcggcgatat gcgcgacccg aggcatcgcc gcgacgtctg
     2521 cccgcgcatc gcctggatgc cgcaggggct gggcaaaaac ctctaccaca ccttgtcggt
     2581 gtatgaaaac gtcgattttt tcgctcgcct gttcggtcac gacaaagcgg agcgggaagt
     2641 gcgaattaat gagctgctga ccagcacggg gttagcaccg tttcgcgatc gtccggcagg
     2701 gaaactctcc ggcgggatga agcaaaaact tgggctgtgc tgcgcgttaa tccacgaccc
     2761 ggaactgttg atccttgatg agccaacaac gggggttgac ccgctctccc gctcccagtt
     2821 ctgggatctg atcgacagta ttcgccagcg gcagagcaat atgagcgtgc tggtcgccac
     2881 cgcctatatg gaagaggccg aacgcttcga ctggctggta gcgatgaatg ccggagaagt
     2941 gctggcaact ggcagcgccg aagagctacg gcagcaaacg caaagcgcta cgctggaaga
     3001 agcatttata aatctgttac cgcaagcgca acgccaggcg catcaggcgg tagtgatccc
     3061 accgtatcaa cctgaaaacg cagagattgc catcgaagcg cgcgatctga ccatgcgttt
     3121 tggttccttc gttgccgttg atcacgttaa tttccgcatt ccacgcgggg agatttttgg
     3181 ttttcttggt tcgaacggct gcggtaaatc caccaccatg aaaatgctca ccggactgct
     3241 gcccgccagc gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa aagatatcga
     3301 tacccgccgt cgggtgggct atatgtcgca ggcgttttcg ctctataacg aactcaccgt
     3361 gcggcaaaac cttgagttac atgcccgttt gtttcacatc ccggaagcgg aaattcccgc
     3421 aagagtggct gaaatgagcg agcgttttaa gctcaacgac gttgaagatg ttctgccgga
     3481 gtcattgccg ctcggcattc gccagcggct ttcgctggcg gtggcggtga ttcatcgccc
     3541 ggagatgtta atcctcgatg agcctacttc tggtgtcgat ccggtggcga gggatatgtt
     3601 ctggcagttg atggtcgatc tctcgcgcca ggacaaagtg actatcttca tctctaccca
     3661 ctttatgaac gaagcggaac gttgcgaccg catctcactg atgcacgccg gaaaagtgct
     3721 tgccagcggt acaccgcagg aactggttga gaaacgcgga gccgccagtc tggaagaggc
     3781 atttatcgcc tatttgcagg aagcggcagg gcagagcaac gaagccgaag cgccgcccgt
     3841 ggtacacgac accacccacg cgccgcgtca gggatttagc ctgcgccgtc tgtttagcta
     3901 cagccgccgc gaagcgctgg aactgcgacg cgatccagta cgttcgacgc tggcgctgat
     3961 gggaacggtg atcctgatgc tgataatggg ttacggcatc agtatggatg tggaaaacct
     4021 gcgctttgcg gtgctcgacc gcgaccagac cgtcagtagc caggcgtgga cactcaacct
     4081 ctccggttcc cgttacttta tcgaacagcc gccgctcacc agttatgacg agcttgatcg
     4141 tcggatgcgt gcgggcgata tcacggtggc gattgagatc ccgcccaatt tcgggcgcga
     4201 tatcgcgcgt ggtacgcctg tggaactcgg cgtctggatc gacggagcga tgcccagtcg
     4261 tgccgaaacg gtaaaaggtt acgtgcaggc aatgcaccag agttggcttc aggatgtggc
     4321 gagccgacaa tcgacacccg ccagccaaag cgggctgatg aacattgaga cgcgctatcg
     4381 ctataacccg gacgtaaaaa gcctgccagc gattgttccg gcggtgatcc cgcttctgct
     4441 gatgatgatc ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag agctagggtc
     4501 gattatcaac ctttacgtga cccccaccac ccgtagcgaa tttttgcttg gcaaacagct
     4561 gccgtacatc gcgctgggga tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt
     4621 gtttggcgta ccgcataaag gcagtttcct gacgctcacc ctggcggcgc tgctgtatat
     4681 catcattgcc accggaatgg ggctgctgat ctccaccttt atgaaaagcc agattgccgc
     4741 cattttcgga acggcgatta tcacgttgat cccggcgaca cagttttccg ggatgatcga
     4801 tccggtagct tcgctggaag ggcctggacg ttggatcggc gaggtttacc cgaccagtca
     4861 ttttctgact atcgcccgcg gaacgttctc gaaagcgctg gatctgactg atttgtggca
     4921 actttttatc ccgttgctga tagccatccc gctggtgatg ggcttaagca tcctgctgct
     4981 gaaaaaacag gagggatgat gcgccattta cgcaatattt ttaatctggg tatcaaagag
     5041 ttgcacagtc tgctcggtga taaagcgatg ctgacgctga ttgtcttctc gtttacggtg
     5101 tcggtgtatt cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc gatcgccatt
     5161 gccgatatgg atcaatcgca gttatcgaac cggatcgtta acagcttcta tcgtccgtgg
     5221 tttttgccac cggagatgat caccgccgat gagatggatg ccggactgga cgccggacgc
     5281 tataccttcg cgataaatat tccgcctaat tttcagcgtg atgtcctcgc cggacgccag
     5341 ccggatattc aggtgaacgt cgatgccacg cgcatgagcc aggcatttac cggcaatggg
     5401 tatatccaga atattatcaa cggtgaagtg aacagctttg tcgcgcgcta ccgtgataac
     5461 agcgaaccgt tggtatcgct ggaaacccgg atgcgcttta acccgaacct cgatcccgcg
     5521 tggtttggcg gggtgatggc gatcatcaac aacattacca tgctggcgat tgtattgacc
     5581 ggatcggcgc tgatccgcga gcgtgaacac ggcacggtgg aacacttact ggtgatgccg
     5641 ataacgccgt ttgagatcat gatggcgaag atctggtcga tggggctggt ggtgctggtg
     5701 gtatcgggat tatcgctggt gctgatggcg aaaggtgtac tgggcgtacc gattgaaggc
     5761 tcgatcccgc tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc
     5821 atttttatgg ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt gattctggtg
     5881 ctgctgccgc tgcaaatgct ttccggtgga tccacgccgc gcgaaagtat gccgcagatg
     5941 gtgcaggaca ttatgctgac catgccgacg acacactttg ttagcctcgc gcaggccatt
     6001 ctctaccggg gtgccggatt cgaaatcgtc tggccgcagt tcctgacgct gatggcaatt
     6061 ggcggcgcgt ttttcaccat tgcgctgctg cgattcagga agacgattgg gacaatggcg
     6121 taaggaaggg aaacttgact tcatactgtg atgtcatact attgatgtca taaagtgaag
     6181 tcataaggac atgccatgag aaccaaggta caggctttac ggaagaaaaa aaaatacatt
     6241 ggatcaaata tttaaaactc ctgttcctca agggatcaag tggtctgata tagagtcact
     6301 ggttaaagca ttaggcggag aaattaagga aggaagaggt tcgcgttgta agttcatact
     6361 aaatatgagc gttgcgtgtt tccatcggcc tcatccgtcg ccagataccg ataaaggcgc
     6421 tgtagaaagc gtgcgtgact ggttactaag tataggggta aaaccatgat caaactgaag
     6481 acgccaaact ctatggagat tgccgggcaa ccagctgtta ttacctacgt gccagaactg
     6541 aatgcatttc gtggcaaatt cctgggcttg tcaggttatt gtgattttgt ctctgacagc
     6601 attcaggggc tgcaaaaaga aggggaactt tccctgcgag agtatcttga ggattgtaaa
     6661 gctgcgggta ttgagccgta tgcgcgtaca gaaaagatta agacctttac gttacgctat
     6721 ccagaatcgc tgagtgaacg ccttaataat gcggctgcgc agcaacaagt ctctgttaat
     6781 acctatatta ttgaaacact gaatgagcgt ttgaatcatt tataaatttt agcggcgtac
     6841 attacgcaga atatctgcct ttacaggatc tatctatgca actgaactta attttataaa
     6901 ttaagttcag ttaatatctt ttctttcctc gtttcagaaa taactcaatc ttccttcggc
     6961 aagcactgca aatgcccatg ccgcacgccg cgctgggcga taacgtcatc ggcaaaatgc
     7021 tgcacgtcgc ccatatcacc tttcagcacg gcgatttcca gacagtcgtc gtgattgata
     7081 tgcacatgca gcgtggcgac ggagagatcg tggtgatggt gctgggtgga gacaatgcgg
     7141 ctggctaagt cgcgtttttc gtgttcatac acataggaga gcaccgcgaa accttgcgtg
     7201 ccgtgctgct gagtggcctc ctgggccagg gcgctacgca gaatatcgcg gatagcttcg
     7261 gaacggttgt tataaccacg acgctggctc aggctgtcca gcgtctccag taaatcgtca
     7321 tcaagcgtga tggtgactcg ttgcatttgc gttaaacctt ttctgtggtg cgacggcgca
     7381 cggggaatgc gggtaatacc gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta
     7441 atttatctcc caccacctgg gtttcgacga tttgtccgtt gtccatcacc atcacccgct
     7501 gacagaagcg ttccaccagg cgtaagtcgt gggtgatgaa caggcaggcg gtgccaaact
     7561 gttgttgtag ctttttcagc aggcgaatga cgcccgcctg taacacgaga tcaaggttag
     7621 aaacggcttc atccagaatc agtagtttcg gttcgaccgc cagcgcgcga gccaggcaga
     7681 cgcgctggag ctggccgccg cttaactgcg gtgggcgttt gtcgagaacg ctgtcatcga
     7741 gatcgaccgc cttcagcatt tcgctggcgc gcgccagttg ttcggatttt ttcagtgata
     7801 gcaggtggcg catcggttca cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc
     7861 tgatggagtc ctgaaatacc atctgaatat cgcggcggaa cgctttacgc tgggcgcgat
     7921 tgagtttcgc cagcggttcg ccacgccagc taatattccc ctgcgcgggc gattctaaac
     7981 ccaccagcaa ccgcgcgagg gtacttttcc cgcagccgct gcgccccagc agggcgacgg
     8041 tttcgccgct tttcagggtc agggaaacgt tattcagcac cgcctgatgt tgatgttttc
     8101 cgttaaatcc accgtgggca tagtgatggg aaaggccgga gacgttaagt aaagtcatga
     8161 tgccagctcc ataccgtaga gggcgagatg agcggaaacc aggctgcgcg ttaccgcatg
     8221 tttgggggcg ttaaacagcg tttctacatc gccctgttcg acaattttac cttgtgacat
     8281 caccgccacg tcatccgcca gacgcgccac aacgcccata tcatgggtga ccagcagcat
     8341 tcccggcgct tgtttttgca taatgctttc cagcagatcg aggatgcgcg cctgtgctac
     8401 cacgtcgagg tcggtggtcg gttcatcggc gatgataaac ggtgattcac acagcaccgc
     8461 catcgcaatc atcatgcgct gcaacatgcc gccgctcatc tcgaacgggt acagcttcag
     8521 cacgcgcgcg gcgttttcca gccccaccgc ttctatggca gcggtaagcg tagcgtcatc
     8581 ggcgggtttc cctaacgcca ggcaggtttc ccgcgcgtgg gtgtgcatgg tgtgtagcgg
     8641 attaaaggcg ctgcgcgggt tctgcatgat ggtggcaatt ttgatgccgc gcagggcgca
     8701 gggataaacc gctttgccat cggctaaaat ttcccccgcc gtctggcgaa cgccagcggg
     8761 caaaatgccc agcgtcgcgg cgcaggttaa tgatttcccg ctgccgctac cgccgactaa
     8821 cgccagcacg cgcccgcgtt gcagggttaa cgatacaccg tgtaccagcg gctgcgcggc
     8881 ctgtagcgcg atattacgta gttcaatctg ttgcggcatt agtgtgcgtg ctccgtcacc
     8941 agatgaggat ccagatgatc gcgcagtgcg tcacccacca ggttaaaggc catcacgctg
     9001 ataaacagcg ccagccccgg ccagaacatt tgcagcggct gggtccagat atactggcgc
     9061 gcgtcgttaa tcatcacgcc ccattcggcg gtcggcgcgg tcacaccgag gccaaggaaa
     9121 gacatccccg cgacgtgcag catcatatgg ccgatatcca gcgttgccag caccagcagc
     9181 gaagggatca ccgcgcctgc cagatgatcg acaaacaccc gcacatggcc cgcaccggaa
     9241 agccgtgacg ccagcacaaa ctcgcgttgg cgtagtgaaa tcaccaagct gcgcaccatg
     9301 cgtgcatacc acgcccagtg cgacagggcg atggcgataa ttacgttggt cagcccggtg
     9361 ccgagcacgc caaccataaa gaacgacaga atcgaggtcg ggaaggtcat aaacatatcg
     9421 gcgacgcgca tggtggcctg atcaacgcgc ccgccaatca acccggcgct gccgccaata
     9481 accagcccta atgtcagcac cagcagcagg caggccatta ccgaaccgag cgacacgcgg
     9541 gtcgctgcca tcagccgcga gaaaatatcg cgacctaagt gatcggtgcc cagccagtgc
     9601 tgcgcatccg gcgaaagcag gcgcgacggc aaatcaatcg cctgggggtc atacggcagc
     9661 caccactggc tggtgagcgc aatcagcgcc agcagggcga taatgatcag cgccaggcgt
     9721 accgaccagc gggaagagag gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc
     9781 gcgggtccag cgcggcgttg agcaaatcga caatcaaatt acagaccaca aaaaccacca
     9841 ccatcatcag cgtaaagcac tgaatcaccg gatagtcacg gttaaaaatc gccgacaccg
     9901 catagcgccc gacgcccggc caggcaaaga tgttttcgat aatcatcgtc ccgccaatca
     9961 gttcgccgat gtgcatcccc acggcggtga tcatcggcag cgaggcattg cgcagaatat
    10021 ggcggcgttc ggtctgtttg tcgttcaggc cgcgcagacg cgcccaagtg acgtgacgct
    10081 gaccggcgac gtccagcata ctggcgcgca gcagtcgcgc gttaatcgcc agcgacataa
    10141 aggcaatgga aaccgcaggc aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta
    10201 gccattgcag atacaccgaa aacgccatca ccagtaaaaa cgccagccag aagttaggca
    10261 tcgacacgcc aagaaacgcg atgaaacgta cggcgaaatc cggcagacgg tcgcgatggc
    10321 gcgccgccca gataccgagc ggtacggaag tgagcagaat taataccagc gccgcacctg
    10381 caagttccag cgtggcgggc aggaagttca gcatatcgtc cagcaccggg cgttggctgg
    10441 cgaatgagat accaaagtca agatgcagcg ccttccacaa ccaggtaccg tactggacgt
    10501 acagcggctg atccagcccg agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg
    10561 gcaggttaga taaacgcaga taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca
    10621 gaaaaatgat caccgaggcg gcgagcacca tcgggatcag cagcagaaag cggcgtaata
    10681 cgtaacgcaa cattaaggtt tcaccggttt aatctgttcg aacggaattt cggtggcgat
    10741 cggcgcgtag gggatattac ccagctccgg ttttgatacc accatcatcg agatgtagct
    10801 gataggcaga taaaccgcct cgtcatgcag acgggtcaaa atgtcgcgat acagcgcctg
    10861 acgttgcgtt tcgtcatggg tcgccagcac ttcgccgatc tctttatcaa tcagcggctt
    10921 gtcggctaat ccttgctgtg cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag
    10981 gaaggcgtgt ggatcgtatg gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc
    11041 gtcgcgctga cgagcataga tactgctctc ttcttcgcca atcagcgaga catctgcgcc
    11101 aatctggcgc atatcagcct gaatgatttc cgccatcgat ttgcttaacg catcggtgcc
    11161 gatgaacgac agttcaatgc gcagcggctg accatttttc tcgcggatgt ctttgcccgc
    11221 aggcagcgtc caaccggctt tttccagcaa ctctttcgct ttctgcggat cgtactggcg
    11281 cggtttcagg ccgagattag cgtaaggcac agaaggggca aacagggtgt cggcgacctg
    11341 ctgggtgcca tataacgcgt tatcaatcag cgattttttg tttaccgcgt aattaagagc
    11401 ttcacgtact gccagctcgt tggtgggggc tttggcggta ttgagcgcca gcatcacggt
    11461 ttcgatcggc tgtgacagtt gggtgtggta agccggattc tggctaaagc gggcgaaggt
    11521 atcgagcggt aataaccctt cgtttccgta cagcaggtcg atatcgccag tttcaaacgc
    11581 caccgcgcgg gtagtcgggt ccgggatgac gttaaaggtg atctttttaa tcgctggctt
    11641 ttcgccccag tagttttcgt tacggacgaa gacatcgtac tgattcagtt tcgattcctg
    11701 caaaatccac ggtccggtgc caatcggcgc tttaattccg ttcatggttt catggttttt
    11761 aaactgcgag ggagcgataa aacggaaagg acggggcagg gccagttctt gcaggaaagg
    11821 atagtaggcg cttttcaggg taatttgcag ctctgtttta ctgagtgctt taacatcaac
    11881 aatctggttt gccagctcca gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa
    11941 gttttctgcc gccgcctcgg catcgaacgg ttcaccgttg gagaatttca cgtcatcacg
    12001 cagggtgaag gtccaggttt taccatcttc tgaatgagtc cagctttttg ccagccacgg
    12061 gatcaccgaa ccgtctgcct gatatttcac caatggttca taaaccatgc tctgggcgaa
    12121 catctggtta ggcgtgtaaa ggtgcgggtt tagtggcccg acattcaccg gccaggcggt
    12181 agtgatttca tctggtgcag cggcatggac gataaaagac gcacaagcca gcagcgcaaa
    12241 tagagtgcgg cggagtgtgg agagcatggt aaccccaatg gattaaaata gatggcggaa
    12301 ataagtatga cgattttaag tattcgtcat actgattacc tgttctggat caatagatgg
    12361 gcagtcgggt gggcggatca gttaactgaa tcgatccatt gcacactgtc ggcggtgagc
    12421 gtaaaggggg tgggggtgca gatcgcaagg cttaaatttt ctagctgaca atggctgact
    12481 gacagcgagg agtgataggt gctatctacg ctgacgattt gccaggcgct gccgccacgc
    12541 tgtttaacga tggcttcttt gcgcgtccag atgcgccaga acatttccag ttgctgatca
    12601 ggatgcacgg cgtccatttc agcgtgttcc ccgaggctga acacggcgtt cgccagccag
    12661 cgccagttgg cgcgcgggcg aatcacttcg atatcgcagc cgacttcgcc ttcatcgctc
    12721 aacagcagag cgatatcgtc accgctatgg cttaagttga accatagcgg cgtttccggc
    12781 gcgaatgcag gtttgccttg ttcgccatag atgatctccg gtagcgggga aagcgtgtgc
    12841 gaaagcaatg cacgccccgc cagccagcgt tcgcgtcgtg gaccttgcgg tgcttgctcg
    12901 cgtaaacccg gtggcagtgg agctgcgctt aaggtcgaaa ctttccccag aactatccga
    12961 tacatatcag ggccaacgtt taatggaaaa tgaaagtgcg tatcgtatca cttgtcgcct
    13021 catcccggta accgactttt cggtctgcct ggccccagta aaatcgccag tttgctaccg
    13081 cctttggtgg tttccatcca gattttacac acactggtta atggcaccga aagtagcatc
    13141 cctaccgggc cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac
    13201 atccccaggc gatggcccat catccgtggt tctaaaatat tgccgatgac catatggacc
    13261 actaaaaaca atgcgccgac cagaatacat tcgtaaacac cattaaacag cagcacctga
    13321 atcattggcg gtacggcgga aattaccgcg ccgatattgg gcacgtagtt gagcaaaaac
    13381 gccagtactg cccacatcag cgcaaactgc acccccatca gctccagccc cagccagacg
    13441 atgacacctg tccataaact gagtagcgtc ttcaatgcaa gatagtgcga aacgccttta
    13501 agtgcgcggt gtaatcctgc gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg
    13561 taagggacgt ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg
    13621 ctcgccattg ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac cactttttcc
    13681 gagtccatcc gctgcagcat tcgctccggc gacatatgca aattaagaaa aggcaacatc
    13741 tcctgcaatt taaaaagttt gcgcgtcagc tccttattaa acttcggcag catagagata
    13801 aattcgttaa acgatgccgc cagtacgccg accagcgcgg ttagtgcgat cagcatcacc
    13861 accactacaa tcgtaatggc aacggggcgt tgtactcccc gacgaataaa ccaggtaacg
    13921 agcgggttga ggacgatggc aaaaaacagc gccagtaaca gctgcacaat gatatctgcc
    13981 gctgcgtgaa tgcccgcgag gatcactacc agcgaggcca gcttgagcag aatgtgcatg
    14041 cccgttttat cgggttgagg gatttccatt ggggcttcct tgtgactttt tgtattaagt
    14101 gtagcgggag tcacgccagc aatattctga ttcttactgc tgattttcac gtcagcacgt
    14161 ggtaaaaatg aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga
    14221 acccgcgcta aacggattgc gcctgaattt gcgcattgtc tccattgtca tgtttaactt
    14281 cgccagctac ctcaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt
    14341 gatgggcttt agcgctttct gggcaggatt ggttatcagc ctgcaatatt tcgccacctt
    14401 gctgagccgt cctcatgccg gacgttacgc cgatttgctg ggccccaaaa agattgtcgt
    14461 cttcggttta tgcggctgct ttttgagcgg tctgggatat ctgacggcag gattaaccgc
    14521 cagtctgccc gtcatcagcc tgttattact ttgcctggga cgcgtgatcc ttgggattgg
    14581 gcaaagtttt gccggaacgg gatcgaccct gtggggcgtt ggcgtggttg gctcgctgca
    14641 tatcgggcga gtgatttcgt ggaacggcat tgtcaattac ggggcgatgg cgatgggtgc
    14701 gccgttaggc gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt taatcattat
    14761 gggcgtggcg ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa
    14821 aggcaaaccg ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg gtatggcact
    14881 ggcactggct tctgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgc
    14941 taaaggttgg gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac
    15001 gcgtttgtta ttccctaacg gcattaaccg tatcggcggc ttaaacgtgg cgatgatttg
    15061 ctttagcgtt gagataatcg gcctgctact ggttggcgtg gcgactatgc tgtggatggc
    15121 gaaagtcggc gtcttactgg cgggggcagg gttttcgctg gtgttcccgg cattgggcgt
    15181 agtggcggta aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt
    15241 atttatggat ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagctgggc
    15301 gggcgtaccg gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac
    15361 gtggcgatta aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa
    15421 cttactgaat aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat
    15481 cgtcagctgg cagagtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa
    15541 cggaagagta cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt
    15601 gacctttcag ctcaatggct ttgttggtta ccacttgcag ctgcggatcg cggctacgtt
    15661 gctgatcttc cagacgcttc gccaacaccg ccagatcttc tttcggatca tcgcccatga
    15721 tgacgatgac tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg
    15781 ttcccagctt accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat
    15841 caagcaggtt gacgggattc ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag
    15901 cgttagtgtc tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat
    15961 atttaaccag attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca
    16021 tccatcttat cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg
    16081 atttatctgc cagtcttttc aacagaatat tgagtaatac gccatacatt ggcaggaaga
    16141 aaacgatact gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt
    16201 cagccataaa ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc
    16261 tgacgttacc gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca
    16321 ggcggttaaa aacgtgcacg tcgaggatct gtcccagcgc gtaggccatg aaactggcgg
    16381 tggcgatacg ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccaggaac
    16441 ccatatagaa tagcgacgag atgacgtagg agatcagcaa cgccgggatc atcaccgcaa
    16501 agatgatacg acgggccagc ggtgcgccaa aaatacgcac ggtcaggtcg gtagcaagga
    16561 aaataaacgg gaagctaaac gcgccccagg tggtatggaa acccaaaatg gagacgggaa
    16621 gctgaaccag atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca
    16681 acgccttata gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat
    16741 tggggtgagg gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta
    16801 cccttttttc gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc
    16861 acgcaatcgt taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt
    16921 tatgtgatga gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac
    16981 ttggcctgcg ctgcccggaa cccgtaatga tggtgcgcaa aactgtgcgc aatatgcagc
    17041 ctggcgaaac gttgctgatt atcgccgacg atccggccac tacccgcgat attcctgggt
    17101 tttgtacctt tatggaacac gaactggttg cgaaagagac ggatggactg ccttatcgtt
    17161 atttgattcg taaaggcggt tgatggggaa agattggctt cgatgccgcc ttttcccctc
    17221 accctaaccc tctccccaga ggggcgaggg gaccgatcgc gctcaatgtt gcgatcggtt
    17281 tgccttatct cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg
    17341 tatctgccag caccgccagc cacaggccgg tcatccctaa cagcgtggtg acgaggaaga
    17401 tccctttcag ccccagcgca atggtgatgt tctggcggat attggcgtga gtggcgcgtg
    17461 ccagttcaat catttgcacc aggccgcgca ggtggttatg ggttaatgct gcatcagcgg
    17521 tttccagcgc cacgtctgtg ccactgccca ttgcaatccc aatggcggca gctttcatcg
    17581 ctggcgcgtc gttaatacca tcaccgacca tcgccagggg cgcatgttga ttcagcttgg
    17641 tcaccgcttt gactttatct tccggcaata ggcccgcttt aaactccagc cccagctccc
    17701 cggcgattgc cgccgctgca cgtggattat cgccggtgag gatcactcct ttgaccccca
    17761 gcgcgttcag ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa
    17821 tgatacccag cacgtcatca ttacgtacta ccagcacaac cgtttgcccg gcgctttcca
    17881 gttcattaat caaaccagca aatgcatcag cgggatgttt cccggcagcg caaatcaata
    17941 cgcgctcacc gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg
    18001 cggtgggaat ggcgagttca gcaacctgtg cttcgcgtac gatggcttgc gccagtggat
    18061 gcgtcgcgcc ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg
    18121 ttgccggatg aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa
    18181 acgccacctg agtaacacga cctagttgtt ccagcgccgc tccgccttta atcaacgccc
    18241 cacgacgcgc tgccgccgcc agcccggagg taatcgccgc aggcgttgag ataactaacg
    18301 cacacgggca gccaatcagc agcagcgtca gccctttata aatccactcc tgccagctgg
    18361 cggcaaacag cagcggtggc accagcgtca ccagcagagc gacggccata atcgcgggcg
    18421 tatagatacg gctgaaacgg tcgataaacc gctcaatggg ggcgcgacgc tcttcggctt
    18481 cttcgatcag tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca
    18541 gcgttaccag acggtctacg ctggtggctc ctgcaggaac tttatcgccc gtcgcgcgct
    18601 ccaccggaat ggattctccg gtcagggcgc tttcatcaaa actggcaaac ggtgagagca
    18661 gtttaccgtc ggcaggcaaa cgcccacctg cggcgacttc aatcacatcg ccaggccgca
    18721 ggctgttaat cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt
    18781 tcagcgccat taacgcgcta acgccctgac gcgcgcggct ggcggcccag ccttccagtc
    18841 gttcaccaat caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca
    18901 gtgcaccaat agcggctacg ctcattaagg tttcaatggc gaagtagctg ccggatttga
    18961 tcagccgtaa tgcctgacga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg
    19021 ccagttgccc gaacggatga ttgaactgct ccagacccca gctgattgcc atcatcacga
    19081 ttagtgtaat cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct
    19141 gctcatcgcg cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacgaatgt
    19201 cattgtcggc atcaaccacc agtttttcgg tggcgaacaa cacctgcacc tgattcacac
    19261 ctgcaagctg gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccac
    19321 tgactttcca gctatagcgg gtgccggaga cgttttcaga gagcgttggc gagctggagc
    19381 atgcgccgtc gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag
    19441 caaattgagg ggctttcttg ccgtgattgt caggtgtcga catggcatac tccggttaag
    19501 ttttttctca ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag
    19561 agatacagtg agcggatgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg
    19621 ttatccgcac ggaagcgtcg gcgatagtgg ctccccagcc agacaaagtt actgataaac
    19681 agcaacgaag cgccaacaaa cgcagagagc gccggagcgg tcggacggaa gaaccacagt
    19741 tcacctgcca gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga
    19801 tactcttcca gccgcgtcca gataatcgcc agtaacagcg cacccagaac cagcaacacc
    19861 agtggcagag gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc
    19921 aggtgcgaga ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt
    19981 agcgcatcgc ccagcagtga ggcgcacagc cctgccagca ccagatagct aatggcgtcg
    20041 aacatcggcg cttgccaggc cagtaacagc aggagaagaa gggttaacgg tttaaacacc
    20101 cagcgttgcc aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag
    20161 acagcgataa acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga
    20221 atgcctacgg tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact
    20281 ggcagggtat gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct
    20341 tttctttatt gttatcattg gcttaattgt cgtcgccgca tcgtttcgtt ttatgcaaca
    20401 gcgacgggaa aaagctgata atgatatggc tccgctccag caaaagctgg tggtggtgag
    20461 caacaagcgg gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc
    20521 aggcaccagt atgcgctacg aggcaagctt caaaccgcaa agcggaggaa tggagcagac
    20581 gtttcgcctc gacgcccagc agtaccacgc cctgacagtg ggcgataaag gtacgctgag
    20641 ctataaagga acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt
    20701 aaatttcttc tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gtaccttttc
    20761 ggcggtggtc atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagctgcat
    20821 accatgcatc agcacggtaa aaatcagcgc cacgttaacg aaaatattta gcgggcgcgg
    20881 gaagggatgc accagattga ggattaaaaa tccccaaacg cagagcatta acaaacgacc
    20941 aatattaatc agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct
    21001 gacccgccac tttttcccga tgtaacgacc agtttgccgg aacggtgggc agaccgtttt
    21061 cgacttcgct ttcgacataa atcagggctt cgtcagccag ccagccgtta tcttccagta
    21121 aatttatcgt ctcttctaac aagccacggc ggaacggtgg atcgacaaac acgatattat
    21181 gcggtgtgcc tttttgcgcc aggaatgaca tcgcgttgct gttcaccacg cgtgcattgc
    21241 ctgcttttag tgtcgccaga ttcttaatta actgctgaga aactgcacga tccatctcaa
    21301 ttaacgtggc ccccgcagcg tagcgcgata acgcttccag ccccagcgcg ccgctcccgg
    21361 cgaagcaatc cagacattgg gcgtcaacaa tgaccggagc cagccagtta aacaacgttt
    21421 cgcgtacgcg gtcggtggtg gggcgcagac ctgggctatc aggaaccggg agtttacggc
    21481 ctcgccactg cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca
    21541 tgataaattg ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt
    21601 aagtgataga ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc
    21661 atgaagcaac agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt
    21721 tcctggctgg gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat
    21781 gaacaaccgg ttgtagaaga aatcgttcag gcgcaagagc ctgtgaaggc ttcggaacaa
    21841 gccgttgaag agcagccgca ggcgcatact gaagccgagg cggaaacttt tgctgccgac
    21901 gttgtggaag tcactgaaca ggttgttgaa agtgaaaaag cgcagcctga agcggaagtt
    21961 gttgcacagc cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa
    22021 gagctgccgt tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct
    22081 gaagcggaaa ccgtagagat tgtcaaagcg gcggaagaag aagcggctaa agaagaaatc
    22141 accgacgaag agctggaagc acaggcgctg gcagccgaag cagcagaaga ggcggtgatg
    22201 gtggttcctc cggcagaaga agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa
    22261 ccaaccaaag aaggtttctt cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat
    22321 ctcggttccg gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag
    22381 gagctggaag agcagctttt gatcgccgat gtgggcgtgg aaaccacacg taaaattatc
    22441 accaatctga cggaaggcgc atcccgcaag cagcttcgtg atgccgaggc gctctatggc
    22501 ctgctgaaag aagagatggg tgagattctg gcgaaagtcg atgagccgct gaatgttgaa
    22561 ggtaaaacgc cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg
    22621 attggtaagc tggcacgtca gtttgagcag cagggtaaat cggtgatgct ggcggcgggc
    22681 gatactttcc gtgcagcagc ggttgaacag cttcaggtct ggggtcagcg caacaatatt
    22741 ccggtgattg cccagcatac cggggcggat tccgcctctg ttatcttcga cgccattcag
    22801 gcagctaaag cgcgtaatat cgacgtcctg attgccgata ccgccggacg cctgcagaac
    22861 aaatcgcacc tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt
    22921 gaagcgccgc atgaagttat gctgactatt gatgccagca ccgggcagaa cgcggtaagc
    22981 caggccaaac tgttccacga agctgttggc ttaaccggca tcacgctaac gaaactggac
    23041 ggcacggcga aaggcggggt aattttctcg gtggctgacc agtttggtat ccctatccgc
    23101 tacattggtg tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga cgactttata
    23161 gaggcacttt ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc agcaaggctt
    23221 atctcggtgg gagacaggcg ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg
    23281 cgtttctgac cggtcattcc ggcgcaggga aaagtaccct cctgaagctg atctgtggga
    23341 ttgagcggcc cagcgccggg aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa
    23401 accgtgaagt tccgtttctg cgccgccaga ttggcatgat tttccaggat caccatctac
    23461 tgatggaccg tactgtctac gataacgtgg cgatcccgct gattatcgct ggtgccagcg
    23521 gtgacgatat tcgtcgccgg gtgtcggcgg cactggataa agtcgggcta ctggacaaag
    23581 cgaagaactt ccctattcag ctttccggcg gtgaacaaca gcgtgttggc attgcccgcg
    23641 cggtggtgaa taagcccgcg gtactgctgg cggacgaacc gaccggtaac ctggacgacg
    23701 cgttgtcgga agggattcta cgtctgtttg aagagtttaa ccgcgttggg gtaaccgtat
    23761 tgatggcaac gcacgacatc aacctgattt cacggcgttc ccatcgcatg ctcaccctga
    23821 gcgatggtca cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat caatcatatt
    23881 cggcagtttg gtgggcgtct ggatcgcttc cgtaaatcgg tcggcggctc aggcgacggc
    23941 ggtcgtaacg caccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac
    24001 gttttcaacg aacaggtgcg ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa
    24061 ccgttcgcca cgtttttaac ggtgatggtt atcgccatct ccctgacgct gcccagcgtc
    24121 tgttatatgg tgtacaaaaa cgttaatcag gcggcgacgc agtattatcc gtcaccgcaa
    24181 atcactgttt acttgcaaaa aacgctggac gatgacgccg ctgcgggcgt ggtggcacag
    24241 ttgcaggccg agcaaggcgt ggagaaagtg aactatcttt ctcgtgaaga cgcactgggc
    24301 gagttccgta actggtctgg ttttggtggt gcgctggata tgctggaaga aaacccgctt
    24361 ccggcagtgg cggtggtgat cccgaaactc gattttcagg ggacggaatc actcaatacg
    24421 ctgcgtgatc gtatcacgca gattaacggc attgacgaag tgcggatgga tgacagctgg
    24481 tttgcccgtc tggcggcgtt gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg
    24541 ttgatggtgg cggcagtgtt cctcgtcatc ggcaacagcg tacgtctgag catctttgct
    24601 cgccgtgact ccatcaacgt gcagaaactg attggtgcga cggatggttt catccttcgc
    24661 ccgttcctgt atggtggcgc actgctggga ttttctggcg cattgttgtc attaattttg
    24721 tcagaaattc tggtgctgcg attgtcatcg gcggttgcgg aagtggcaca ggttttcgga
    24781 acgaagtttg atatcaatgg tttatcgttc gatgaatgcc tgctattgct gctggtatgc
    24841 tcgatgattg gctgggtggc agcgtggctt gccacggtac aacatttacg ccactttacg
    24901 cctgaataat aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag cagggaaaga
    24961 gatcccgttg tctcttcccg gtatttcatc tctatgtcac attttgtgcg taatttattc
    25021 acaagcttgc attgaacttg tggataaaat cactgtctga taaaacagtg gatgataacc
    25081 tcgttgctct taagctctgg cacagttgtt gctaccactg aagcgccaga agatatcgat
    25141 tgagaggatt tgaatgactg acaaaatgca aagtttagct ttagccccag ttggcaacct
    25201 ggattcctac atccgggcag ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc
    25261 gctggctgaa aagctgcatt accatggcga tctggaagca gctaaaacgc tgatcctgtc
    25321 tcacctgcgg tttgttgttc atattgctcg taattatgcg ggctatggcc tgccacaggc
    25381 ggatttgatt caggaaggta acatcggcct gatgaaagca gtgcgccgtt tcaacccgga
    25441 agtgggtgtg cgcctggtct ccttcgccgt tcactggatc aaagcagaga tccacgaata
    25501 cgttctgcgt aactggcgta tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt
    25561 cttcaacctg cgtaaaacca agcagcgtct gggctggttt aaccaggatg aagtcgaaat
    25621 ggtggcccgt gaactgggcg taaccagcaa ggacgttcgt gagatggaat cacgtatggc
    25681 ggcacaggac atgacctttg acctgtcttc cgacgacgat tccgacagcc agccgatggc
    25741 accggtgctc tatctgcagg ataaatcatc taactttgcc gacggcatcg aagatgataa
    25801 ctgggaagag caggcggcaa accgtctgac cgacgcgatg caaggtctgg acgaacgcag
    25861 ccaggacatc atccgcgcgc gctggctgga cgaagacaac aagtccacgt tgcaggaact
    25921 ggctgaccgt tacggtgttt ccgctgaacg tgtacgccag ctggaaaaga acgcgatgaa
    25981 aaaactgcgc gctgctatag aagcgtaatt tccgctatta agcagagaac cctggatgcg
    26041 agtccggggt ttttgttttt tgggcctcta caataatcaa tttcccctcc ggcaaaacgc
    26101 caatccccac gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatc
    26161 gggtatgttt tagcagagta tgctgctaaa gcacgggtag ccatgcataa aacgaaataa
    26221 agtgctgaac aacaacatca caacacacgt aataaccaga agaatgggga ttctcaggat
    26281 gaacataaag ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc
    26341 tctggcagaa gatattaaag tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta
    26401 cggtgaccaa gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg
    26461 cattaaaggc aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc
    26521 ggttgcggtg gcgaacaaag tcgttaacga cggcattaaa tatgtgattg gtcacctctg
    26581 ttcttcatca acgcagcctg cgtctgacat ctacgaagac gaaggcattt taatgatcac
    26641 cccagcggca accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac
    26701 cggcctggac tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc
    26761 gcagcgtatt gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cacgagcggt
    26821 gcaggacggc ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg
    26881 ggaaaaagat ttctctacgc tggtggcgcg tctgaagaaa gagaatatcg acttcgttta
    26941 ctacggcggt taccacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggct
    27001 gaaaactcag tttatggggc cggaaggtgt ggctaacgtt tcgctgtcta acattgcggg
    27061 cgaatcagcg gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcaaa
    27121 caaacccatt gttgacgcga tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg
    27181 gaccacctac gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc
    27241 tgaaatcgcc aaatacctga aagcgaactc cgtggatacc gtaatgggac cgctgacgtg
    27301 ggatgagaaa ggcgatctga aaggctttga gttcggcgta tttgactggc acgccaacgg
    27361 cacggcgaca gatgcgaagt aatcattaat cggcaacttt gggttgccgc caaattgcta
    27421 atatcgagta cgttgcttca tgccggatgc ggcgtaaacg ccttatccgg cctataagat
    27481 ccaaagaaat cagtaaattg caacacacat tgtaggcctg ataagcgtag cgcatcaggc
    27541 aatttctcgt tggtcatcac ttttgaaatc aacttgacga ttaacgcttc tcccagccgc
    27601 cctgttgcgc cgtaaatccc agtgcctgca taaacgctgt catcacaccg cgatcttcca
    27661 cgcctgcatc cgctatccac cagcatgaaa cgccaggatt gttacgcaaa acctcttcca
    27721 gcagatattg ccccacaccg cgacgtcggg tgacttcccg cacacgcagg gaatccagtg
    27781 ctccctcggt gccgcttaag gttacccgta cggcagcgag caggcgctcg ttaaaacgcg
    27841 cggcgtagat acggtggtta tcgtcaacct gtaaagagga aggggaatac tccggccaga
    27901 tcttttgcag gtcaatccgg tcctggtcgc taaagttttc taatcgaatg atggtcagct
    27961 tcatgggtaa cccgtgtaaa tcacaaaagt ggaaccagtg tagcgaaata atttaatcgg
    28021 aggctttctc ttttttattt ctt
//
LOCUS       DAZRUF010000026        27853 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZRUF010000026 DAZRUF010000000
VERSION     DAZRUF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.026"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            67..729
                     /gene="acrS"
                     /locus_tag="ACHXOL_000924"
     CDS             67..729
                     /gene="acrS"
                     /locus_tag="ACHXOL_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR6807975.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(732..911)
                     /locus_tag="ACHXOL_000925"
     CDS             complement(732..911)
                     /locus_tag="ACHXOL_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR6807976.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(995..1879)
                     /gene="yhdJ"
                     /locus_tag="ACHXOL_000926"
     CDS             complement(995..1879)
                     /gene="yhdJ"
                     /locus_tag="ACHXOL_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR6807977.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1965..2261)
                     /gene="fis"
                     /locus_tag="ACHXOL_000927"
     CDS             complement(1965..2261)
                     /gene="fis"
                     /locus_tag="ACHXOL_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR6807978.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2287..3252)
                     /gene="dusB"
                     /locus_tag="ACHXOL_000928"
     CDS             complement(2287..3252)
                     /gene="dusB"
                     /locus_tag="ACHXOL_000928"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR6807979.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3581..4462)
                     /gene="prmA"
                     /locus_tag="ACHXOL_000929"
     CDS             complement(3581..4462)
                     /gene="prmA"
                     /locus_tag="ACHXOL_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR6807980.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4474..5925)
                     /gene="panF"
                     /locus_tag="ACHXOL_000930"
     CDS             complement(4474..5925)
                     /gene="panF"
                     /locus_tag="ACHXOL_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR6807981.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5915..6157)
                     /locus_tag="ACHXOL_000931"
     CDS             complement(5915..6157)
                     /locus_tag="ACHXOL_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR6807982.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6266..7615)
                     /gene="accC"
                     /locus_tag="ACHXOL_000932"
     CDS             complement(6266..7615)
                     /gene="accC"
                     /locus_tag="ACHXOL_000932"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR6807983.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7626..8096)
                     /gene="accB"
                     /locus_tag="ACHXOL_000933"
     CDS             complement(7626..8096)
                     /gene="accB"
                     /locus_tag="ACHXOL_000933"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR6807984.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9074..10048)
                     /locus_tag="ACHXOL_000934"
     CDS             complement(9074..10048)
                     /locus_tag="ACHXOL_000934"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR6807985.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10200..12140
                     /gene="csrD"
                     /locus_tag="ACHXOL_000935"
     CDS             10200..12140
                     /gene="csrD"
                     /locus_tag="ACHXOL_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR6807986.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12445..13488
                     /gene="mreB"
                     /locus_tag="ACHXOL_000936"
     CDS             12445..13488
                     /gene="mreB"
                     /locus_tag="ACHXOL_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR6807987.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13554..14657
                     /gene="mreC"
                     /locus_tag="ACHXOL_000937"
     CDS             13554..14657
                     /gene="mreC"
                     /locus_tag="ACHXOL_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR6807988.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14657..15145
                     /gene="mreD"
                     /locus_tag="ACHXOL_000938"
     CDS             14657..15145
                     /gene="mreD"
                     /locus_tag="ACHXOL_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR6807989.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15154..15747
                     /gene="yhdE"
                     /locus_tag="ACHXOL_000939"
     CDS             15154..15747
                     /gene="yhdE"
                     /locus_tag="ACHXOL_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR6807990.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15737..17206
                     /gene="rng"
                     /locus_tag="ACHXOL_000940"
     CDS             15737..17206
                     /gene="rng"
                     /locus_tag="ACHXOL_000940"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR6807991.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17274..21074
                     /gene="yhdP"
                     /locus_tag="ACHXOL_000941"
     CDS             17274..21074
                     /gene="yhdP"
                     /locus_tag="ACHXOL_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR6807992.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21230..22675
                     /gene="tldD"
                     /locus_tag="ACHXOL_000942"
     CDS             21230..22675
                     /gene="tldD"
                     /locus_tag="ACHXOL_000942"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR6807993.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22803..23732)
                     /gene="aaeR"
                     /locus_tag="ACHXOL_000943"
     CDS             complement(22803..23732)
                     /gene="aaeR"
                     /locus_tag="ACHXOL_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR6807994.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23915..24118
                     /gene="aaeX"
                     /locus_tag="ACHXOL_000944"
     CDS             23915..24118
                     /gene="aaeX"
                     /locus_tag="ACHXOL_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR6807995.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24126..25058
                     /gene="aaeA"
                     /locus_tag="ACHXOL_000945"
     CDS             24126..25058
                     /gene="aaeA"
                     /locus_tag="ACHXOL_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR6807996.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25064..27031
                     /gene="aaeB"
                     /locus_tag="ACHXOL_000946"
     CDS             25064..27031
                     /gene="aaeB"
                     /locus_tag="ACHXOL_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR6807997.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27123..27395
                     /locus_tag="ACHXOL_000947"
     CDS             27123..27395
                     /locus_tag="ACHXOL_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR6807998.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27451..27714)
                     /gene="yhcN"
                     /locus_tag="ACHXOL_000948"
     CDS             complement(27451..27714)
                     /gene="yhcN"
                     /locus_tag="ACHXOL_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR6807999.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6692 a         6970 c         7393 g         6798 t
ORIGIN      
        1 tgatagatta accttcgcta ttttctcacc ctgtgtcgaa tatatttatt tcctgaataa
       61 ttaatcatgg caaaaagaac caaagccgaa gctctgaaga cccggcaaga actgattgaa
      121 actgccatcg cccagtttgc gcaacatggc gtaagcaaga cgacgttcaa cgacattgcc
      181 gatgccgcta acgttacgcg tggcgctatc tactggcact tcgagaacaa gacccaactg
      241 tttaatgaga tgtggttgca acagccttca ttgcgggagt taatccagga acacttgacg
      301 gctggattag agcatgaccc gtttcaacaa ttgcgtgaaa aattgattgt cggcttgcaa
      361 tatattgcca aaattccccg ccagcaggcg ttgctgaaaa tcttatatca caaatgtgaa
      421 tttaatgatg ggatgctggc cgagggagtg atacgcgaaa agatgggctt taatccgcag
      481 actctccgcg aagtattgca ggcgtgtcag caacaaggtt gtgtagcaaa taacctcgat
      541 ttagatgttg tgatgattat tattgatggt gccttcagcg gaattgttca aaactggtta
      601 atgaatatgg cgggttatga tctttataaa caagcacccg cgctggtcga taacgtatta
      661 agaatgttca tgccagatga aaacataacg aaattaattc atcaaacgaa tgaattaagt
      721 gtcatgtaaa ttcacattct gtcctgaaaa ttcagttcgt cacagttaac cagttttagt
      781 ggataaactg agttcatatt acggcatttg tctgtttctg ttgtcagcag ttcaatccac
      841 tgcatcagta aagtatcaaa cagaaaaacg aagacagcga aaacgacgag ccaccaatac
      901 ttgcgaatca ttacctgagc ctgtgcatgt ttaaccaagt ctggcaggaa atatacacga
      961 aatgaataat ctgaaaacca ggcttacact tcctttactt tgcaatgaga tcggggtcaa
     1021 cttcgcttaa ccggcttcgt tttgacaggt tgccggtctt tctttttttc actttcgcca
     1081 gttcttccgc ggagtaatgc gacgcgacat ccagccgtcg aagccccatt ttgatgtact
     1141 cgctgttgat ctcaatacca atgaattttc gtccgctggc gatggctacg gcaccggtag
     1201 taaagctacc ggcaaacggg tcgagaacga tatcgcctgg gttggaagag gcgagaataa
     1261 tgcgtttcag taaggcttcc ggtttttgcg tcgggtggtt ctcatattca tccatcaaat
     1321 aacgcacgcg cggaaaatcc cagacgttac ccggtacttt ttgatgattg tatggctgtg
     1381 gaggattttt gcgataatcg atcaacgcgc gctgcgatcc ggttttggct tctaccagaa
     1441 tagcatcacc gttgaatgtg tagttctttg cgtctttcac catcatcagg atgggttcgt
     1501 acatggagcc gtagtgtttt ttcgcctgta ctccagaact gtcatatgac cagacgatgc
     1561 gacttttgat ggtaaaaagc ttgcggcact ggagatcgat aaagggcatg ttttccgtac
     1621 tgttcataat gtacatgctg ccctgctttt tcagaacgcg gtggcactct acaatcactt
     1681 caaacagcca gtcgataaac agatcttctt tccaggcttc gatcagacca tcaaaatttt
     1741 taccgatgtt atacggtggg tcggcaaaga tcagatcgac gctttcagta ggtagctttt
     1801 taagttcggc aagggcatca ccgtgaataa tggtcttagc ttcattacca aaccgggtcg
     1861 gttcacatcc tgttctcatg gtcactccct ttgtgacacc tataaaaaag gcgcttcccc
     1921 atgccgagta gcgccttttt aatcaagcat ttagctaacc tgaattagtt catgccgtat
     1981 tttttcaatt ttttacgcag cgtaccacgg ttgatgccca tcatcagcgc agcacgggtc
     2041 tggttaccac gggtgtattg catcaccatg tccaacaggg gctgttctac ttcagccagt
     2101 accagctcat agaggtcatt cacatcctga ccattcagtt gagcaaaata gttcttcagt
     2161 gcctgtttaa ccgagtcacg caggggtttt tgggttacct gatcctgaga gttaacggta
     2221 gaaacggtca gtacgtcaga atttacgcgt tgttcgaaca tagttctgtc agctctttat
     2281 ttctgtttac gcaaaatttt cgaagtatgc ctccaacgcc tccagctgtt cgctggcatc
     2341 ctcaatggcg ttgaatgtgc gccgaaactg gtcatttgga gcgtgttcct ggagatacca
     2401 ggaaacgtgt ttacgtgcaa ttcggtaccc ttttgccgga ccataaaagt catgcagttc
     2461 ccgaacgtgc gcgcaaagca agcgcttaac ctctgccaaa ggcagcgggg gcagcaactc
     2521 cccagtgtcc agataatgct ggatttcccg aaagatccag ggtcttccct gagctgcgcg
     2581 gcctatcatc agggcatccg cccctgtata gtcgagcaca gctctggctt taagcgggtc
     2641 agtaatgtcg ccattcgcga taaccggaat ggaaactttc tgcttaactg cccgaatact
     2701 gtcgtactca gcttctccat tgaacaaaca ggcgcgtgta cggccatgaa tggtcagagc
     2761 ctgaatgcca cagtcttcag ccagttgggc aatctcttcg cagttacggt gttccggtgc
     2821 ccagccggtg cgaatcttca gggtaacagg aacgtccact gcattgacga cctcggtaag
     2881 gatcgattta acgacatccg ggtactgcaa gagggctgaa cctgcgagct tgcgattcac
     2941 ttttttagcc gggcaaccca tattgatatc aataatctgg gcaccgcttt ccacgttaat
     3001 acgtgctgca tctgccattt ctttcggatc gctaccagca atttgcacgg tgcgaatacc
     3061 gggttcatca atgtgcacca tccgtaaacg agatttgtcg ctttcccaaa cctgtgggtt
     3121 agaagacatc atctcggata ctgtcaatcc ggctcccatc tcgtagcaca acgtccgaaa
     3181 aggtctgtct gtaatgccag ccatgggcgc tgcgatcagg cgatttctga gctgatattg
     3241 tccgatgcgc atgagttaag aaatgaccat actgtgactg caaggcggcg tatattacgc
     3301 attttttgca cgagatgaaa ggccaaactt tgaccaatcc tctgagatgg atcaaagaat
     3361 tgcatttaaa atgagcgtgg tgagataatt actcataaaa atcatcatat tagaaaatag
     3421 tgactaaaat ttacactcaa agaaatttga gtaagttctc aatttttctt tatgaatgaa
     3481 aattttggca cgcaaatttg cgtaaataac cggcaaattt acgtgccttt tgtgagcttg
     3541 ctcgcacttc gccccgcgtc accctacggc gatgcgaagg ttaattctta cgaccggtaa
     3601 tgcggcacca ctcttctttt tccacgaccg ggtccagtgc gaagctgtcg acataagctt
     3661 cacaaacgct ctctgcctgg cttgccagaa taccggaaag gcccagcaaa ccgcctgaaa
     3721 ccggcaggac gctgattaac ggtgccagtt cacgtaatgg gcctgcaagg atgttagcga
     3781 ccaccacgtc ggctttcatt tcttctggct gatctttcgg taagtagagt tccagacggt
     3841 cagaaacgcc attacgttcg gcgttgtcgc ggctagcctg aatcgcctgc ggatcgatat
     3901 caataccaat ggcttttgct gcacccagtt tcagcgccgc gatagccaga atgccggaac
     3961 cacagccaaa gtcgatgact attttaccgg ttaaatcgag gctgtcgagc cactgcaggc
     4021 acagagaggt agttggatgg gtacccgtac caaacgccag ccctggatct aacatcacgt
     4081 tgacggcgtt ttcgtccggc acatcacgcc agctagggca gatccacagt cgttcaccaa
     4141 agcgcatcgg gtggaaatta tccatccatt cgcgctccca gtctttatct tccagttgtt
     4201 cgattttatg cgcgaagcct gcgccgagca gcggatggtt ttccagaatc gccaccacgt
     4261 cgttcatatc ggtttcagcg tcgaacagac caatcacatc ggtgtcgccc cacaggcgcg
     4321 tttcgcccgg cagcggctca aatactggcg tatcgtgggt atcctgaaaa gtgatagaaa
     4381 cggcacccgc ttccatcagc gcatcgctaa gatcttcagc gttcgcgccg gtggtgttca
     4441 gtttcagttg gatccaaggc atggcaaaac tctttattta tcagtagtca aaacggtagc
     4501 ttgcgggacg gatgtaccga aacggtttcc gaccaggaaa gccagcaaac ttagtagtaa
     4561 cgagggcacg atagggtgga agcccaggta ctgaatattc agcgtcgcga gtacggcata
     4621 cagcacgccg ccaacgatca tcgcacttag cgcgcctttg gcgttggcgc gttcccagta
     4681 aagacccagc accagcggcc acaggaaaac ggcttccagc ccaccgaagg ccagcaaatt
     4741 cagccagatg atcatttctg gcggcttcca ggcggcaagc agcagcaacg cgccgagaac
     4801 taacgtaatt accgccgaca tccgcttcag acgcgtctcg ttttgcattt gatccggacg
     4861 gatattcaga tagagatctt taatgatcgt agcggaactt tgtagcaatt gggcgttaat
     4921 tgtcgacatg atcgcagcca taggtgcagc cagaaagatc ccggcagcaa acggtggcag
     4981 cacttttacc attaacgtcg ggatcaccag atccggtacg gtgagatcgg ggatcactgc
     5041 acgacctaac gctccggcta ggtgcatacc gaacatcagg attgcgacta caatcgtacc
     5101 gatgatgatc ccccggtgta ccgctttgct gtctttataa gagatacagc gcaccgcagt
     5161 atgcggcagg ccaatcacgc caaagcacac cagtacccag aacgacgtca taaaggcagg
     5221 cgacagaata tcgtcagcgc cttgtggcgt aaccagttgc ggatcgatgg tttgcaaggt
     5281 ctgtactgcg ttgcttaagc cgccagcggc atgtaccacg ccaataagca gcacaacggt
     5341 gccaatcagc atcacaagcc cttgcatggt gtcgttcagc acgctggcgc gaaagccgcc
     5401 aaaggcggta tacaacgcaa tgctgatacc gaaaatcagc agcccggttt cataaggaat
     5461 acccgccgcg gtttccagca ggcgcgcacc gccgataaac tgcacggtca ttgccccaac
     5521 gaacgcaacc agcaaactca aactcgccag ccacaccaga agacgactct ggtagcgggc
     5581 aaacagcatg tcgttcagcg tcactgcatt gtagcggcgc gcaagaatcg caaacttctt
     5641 gccgagaata ccgagtgaaa gccagactgc aggaagctga atcatcgcca gcaataccca
     5701 gcccagcccg tatttataag cagctcctgg cccgccgata aacgaactgg cactgatata
     5761 ggtcgcggtg agcgtcatcg ccagcacaat accgcccata gagcggctgc cgaggaaata
     5821 ctcattaagg aaggtgccgg tgctccgttt acgcatcgca taaaccgaga taccgaacac
     5881 caccaccaga taggcgacca gcggtagaat tacttcaagc tgcatcgtca tcctccagtg
     5941 ggatatcgcg atagataaat ttcaccatcg cccagcacag tccaataaac agtagcggcg
     6001 tcaggatgca ggccatctca aaccagcgcg gaaagccggt aaaaccgggg gcaacgccag
     6061 gtaagtaagc ggctactaac caaactgcca gatacaaaag ggtcagcccc agcgcccagc
     6121 gcgcctcttt atgggcctga acaaaacgag tgtccatttt ttgtccctga tgggtgaaga
     6181 aagcgaggat tgtaccttat gggggttgtc gatccccagt aataaaaaag gccggaaaat
     6241 ccggcctttt gacgcttttg cagtcttatt tttcctgaag accgagtttt ttctccagat
     6301 agtggatgtt agtgccacca tgctggaagt tctcgtcatt catgatgcgg atctgcagat
     6361 caacgttggt tttgataccg tcgatgatca gctcctgcag cgcattcttc atgcgggcaa
     6421 tcgccacgtc acggttttca ccgtagcaaa tcagcttacc gatcattgag tcatagtacg
     6481 gcggtacggt gtagcccgcg tagatatgag actcccaacg tacgccaaaa ccgccaggtg
     6541 cgtggaaacg ggtgattttg cctgggcttg gcaggaaggt gttcggatct tcggcgttga
     6601 tacgacattc caccgcatgg ccgcgaacgt gaacttcttc ttgcttgatc gacagcggtt
     6661 gaccggcagc gatacggagc tgttctttga tcaggtcaac gccagtgatc atttcggtaa
     6721 ccgggtgttc tacctgaata cgggtgttca tttcgatgaa atagaactcg ccgttttcga
     6781 acagaaactc gaaagtacct gcaccgcgat agccgatatc aacacacgct ttagcgcaac
     6841 gttcgccgat gtagcgacgc agttccgggg taatgcccgg tgctggcgct tcttcgacca
     6901 ctttctggtg gcggcgctgc atggagcagt cacgttccgc cagatagata gcgttgccct
     6961 gaccgtcagc cagtacctga atctcgacgt ggcgaggatt ttccaggtat ttctccatgt
     7021 aaaccatatc gttgctgaaa gcagctttcg cttccgcacg ggtcatggag atggattgtg
     7081 ccagttcagc gtcgccgcgc actacgcgca taccgcgacc accgccgccg ccggaggctt
     7141 tgataatcac cggataacca atgcgtttag caatggcacg gtttttatcc atatcgtcgc
     7201 ccaacgggcc gtcagaaccc ggtacgcaag ggacgcccgc ttttttcatc gccgcgattg
     7261 cggatacttt gtcgcccatc aggcgaatgg tttctgcttt cgggccgatg aagataaagc
     7321 cggagcgttc aacctgctcg gcaaagttgg cgttctcgga gaggaagccg taacccggat
     7381 ggattgctac tgcgccggtg atttcagcgg cgctgatgat tgccgggatg ttcagataac
     7441 tttttactga cggagcaggg ccaatacaga ccgtttcatc tgccagtaat acgtgtttta
     7501 gatcgcgatc cgcgctggag tgcacagcga cagtcttgat gcccagttct ttacaggcac
     7561 gaagaatacg caatgcaatc tcgccgcggt tggcaataac aattttatcc agcatgttcg
     7621 cctcgttact cgatgacgac cagcggctcg tcaaattcta ccggttgtcc actttcgacc
     7681 agaattgctt tcacggtacc ggatttgtcc gcttcgatct ggttcatcat tttcatggct
     7741 tcaacgatgc acagggtatc gcccacgttg actttctgac ccacttcgat gaacgctttt
     7801 gcgtccgggc ttggggtgcg gtagaaagta ccaaccatcg gggaacgtac gatgtgacca
     7861 ctgatttccg ctgctgctgg cgcttccatg gaaggaacgg tcgccggagc ggctgcgtta
     7921 gattgagctg gctgctgcat cattggtgca gcgtaagctt gttgcatcac agggaaactt
     7981 gcggcaggag ctgcacggct aatgcgtact gactcttcgc cttcagaaat ttccagttcg
     8041 gagatgcctg attcttcaac cagctcgatc agttttttaa tcttacgaat atccatgagt
     8101 gggttccgta ctctttgttt agtgtgattg tgacaggcgt ttcaccgccg tctgtaaagc
     8161 gtatgccccg acagctgtat gcatagcgat aaattccagc aggccgggac gccggtctat
     8221 tttgccccag agtatcaatg ttagacttga cggacattgt gcagcgatct gctgctatcc
     8281 tccggcaaaa aacaaaatat accttcgttg cgccaatgtc accttttccg cagcgcaaaa
     8341 actgcgtcag ggaggacgag gtcgcacatt ataacgattt cgtagcaatt ggcagctaaa
     8401 tactggtctc atcagggaag ataatcaaca gctaacatgt aaataacctt caacaccgtg
     8461 taatttgcaa caagccgcac aattcacgaa attagcgcca tcatcgacgg aacttcttat
     8521 aacgtaaggt gaaaagctgt aaagccagcc ctgcgaagat gaggggctgc aatgagataa
     8581 tcttcacaga ccacaaataa tgtagggatg ccaggatcgc gacaagatag acgacattac
     8641 gcagcggttg ctggggatta cccaattttc actgcatcgt ctggatagat gtgaatgcta
     8701 aagcacgcaa aatcaaccga ctgacaattc tcagcattaa ataggttcga gtggttaatt
     8761 tgtagtcaaa taaaatcaga ttattcaccc catggtacca gaaatcagta acaggttaag
     8821 cgtagtgtcg cccaggcaaa gcaccataaa cctaacaggc gacggaggcg tatcaataat
     8881 ggctgtttag aggagtatca atgcgcatca atttgtggtc agtcgaaatc atgactttcc
     8941 agtttggatg atgttgtaca acttcctgac caaggtgttc gacgaaagct gtccggctaa
     9001 ccgcttttcc cgttaaccga ttgaataatg atgtcaaaat gagttccctc tcttattatt
     9061 ccttgctaaa tggttagtta accttcacca gcgtgcgacc ctggatctgg ttattaatga
     9121 tggcctcggc aaagttcggt gcctctgaca gagatatctc ttttgccgcc tgggtataga
     9181 atgattccgg taaatcggcg accagtcgct gccaggcttg tgcgcggcgt tctggtggcg
     9241 tcattactga atccacccct tgcaaacgga cattacgcag aataaatggc atgactgtgg
     9301 ttggcagagt aaaaccaccc gccagaccac aggccgccac gcagccgccg taattcattt
     9361 gcgccagcac ttttgccagc actttgtcgc caacagtgtc aattgcccca gcccagactt
     9421 gtttttccag aggacgggat tcggcaaact catcacgagg gagaatacgg ctggcaccta
     9481 aacttttcag atattcatgg gtactttcgc gaccggaaac ggcaacgacc tgataaccca
     9541 acttatgcag cagcgccacg gcggtactgc cgacgccacc actggcaccc gtcacgacaa
     9601 tctccccgtc ctgcgggcga acaccggcat cttccagcgc catcacacac agcatggcgg
     9661 taaaaccggc agtaccgata atcattgctt tacgcgcgtc cagcccttgc ggcatggcaa
     9721 ccagccagtc acctttcact cgcgcctgct ccgccagccc accccagtgg ttttcaccaa
     9781 cgccccagcc agtgagtaac acctcctgac cggcatgaaa acgcggatct tcgctggtgc
     9841 gtacagttcc ggcaaaatcg atcccaggaa tcatcggaaa attacggatg atttttccct
     9901 taccggtaat cgccagcgca tctttatagt tcaggctcga ccagtgaaca tcgaccgtga
     9961 catcgccctc cggcaggcga ctttcgtcca gagtctgtac tgatgcgaga gttttgccgt
    10021 cctgctgttc taaaagtaac gcctgcataa gtggtcctca tgtgcatgat ggattggaaa
    10081 ataatttctg aagattatac tcgctaatgg aaaagcaaag ccgatgaagc gcaataaatt
    10141 gcgagataaa tctgatttgc tagtatgccc gcttcctcac tatcggagtt aacacaagga
    10201 tgagattaac gacgaaattt tcggcctttg ttacgctgct caccgggtta acaatttttg
    10261 tgactttgct gggctgttcg ctaagtttct acaacgcaat tcagtataag tttagtcatc
    10321 gcgttcaggc ggtggcgacg gcgattgata cccaccttgt gtcgaatgac ttcagcacat
    10381 taaggccaca aattaccgaa ttaatgatgt cggcagatat cgttcgtgta gacctgctcc
    10441 atggtgataa acaggtttat accctggcca gaaatggtag ttatcgtccg gttggctcca
    10501 gcgatctgtt tcgcgaactg agtgttccgt tgataaagca tccggggatg tcgctgcgtc
    10561 tggtttatca ggatccgatg ggcaactatt tccattcttt aatgactacc gcgccgctca
    10621 cgggggcgat tggctttatc attgttatgc tcttcctggc ggtacgctgg ttacaacggc
    10681 aacttgccgg gcaagaattg ctggaaaccc gggctactcg tatcttaaac ggtgagcgtg
    10741 gctctaatgt gttgggaacc atctatgaat ggccgcccag aaccagcagt gcgctagata
    10801 cgctgcttcg tgaaattcag aacgcacgcg aacaacacag ccgtcttgat acgctgatcc
    10861 gctcttatgc cgcccaggac gtgaaaaccg gcctcaataa ccgactcttt ttcgataatc
    10921 agttagcaac gttactggaa gatcaggaga aagtaggtac ccacgggatc gtgatgatga
    10981 ttcgtctgcc ggatttcaat atgttgagcg atacttgggg gcacagccag gttgaagaac
    11041 agttcttcac tctgactaat ctgctgtcga catttatgat gcgctaccct ggcgcactgc
    11101 tggcgcgtta ccaccgcagt gattttgctg cgctgttacc gcaccggacg ttaaaagagg
    11161 cagagagcat cgccggtcag ttaatcaaag ccgtcgatac cttgccgaac aataaaatgc
    11221 tcgatcgcga cgatatgatc cacattggta tctgcgcctg gcgtagtggt caggataccg
    11281 agcaggtaat ggaacatgca gagtctgcca cgcgtaatgc gggattgcag ggcggcaata
    11341 gctgggctat ttacgatgac tcgttgcctg aaaaaggacg cggtaatgtt cgctggcgta
    11401 cgcttatcga gcaaatgctc agtcgcggcg gcccgcgcct ttatcaaaaa ccggcggtta
    11461 ctcgcgaagg tcaggttcat catcgcgaac tcatgtgccg catcttcgat ggtaatgaag
    11521 aggttagctc ggcggagtat atgccgatgg tcttgcagtt tggcttatcg gaagagtatg
    11581 accgtctgca aatcagccgt cttattccac tattgcgtta ctggccagag gaaaatctgg
    11641 cgattcaggt taccgttgag tcgctgattc gcccgcgttt tcagcgttgg ctgcgcgata
    11701 cgttaatgca atgtgaaaaa tcacaacgaa aacgcataat tattgaactt gcagaggccg
    11761 atgtaggtca acatatcagt cgtttacaac ctgttattcg tttagtgaat gctttagggg
    11821 tacgggtagc cgtcaaccag gctggtttga cgctggtaag caccagttgg atcaaagaac
    11881 ttaatgttga gttactcaag ctccatccgg ggctggtcag aaacattgag aagcgaacgg
    11941 agaaccagct gctggttcaa agcctggtgg aagcctgctc cgggaccagc acccacgttt
    12001 acgccaccgg cgtgcgttcg cgaagcgagt ggcagaccct gattcagcgc ggtgttacag
    12061 gcgggcaagg ggattttttc gcgtcctcac agccacttga tactaacgtg aaaaaatatt
    12121 cacaaagata ctcggtttaa cctgccgttt aatccgtttt cacgtagaat aacgcgcgct
    12181 gcgtctcatg ggagtgtgct tgtctgctcg ccagattgtt gcagcacata tgcagatgaa
    12241 tgaccttacg cggttgcaaa caggcgagga atgctgctga tgcattaagc ctttctggac
    12301 tcaggcagag atttgtaaca aaggaaacga actgcactaa ttttcaccgt agcagatgat
    12361 ttttgcgcct tgtcgctgct gcgtgtggtt ggtaaagtaa gcggattttc ttttccgccc
    12421 cagctttcag gattatccct tagtatgttg aaaaaatttc gtggcatgtt ttccaatgac
    12481 ttgtccattg acctgggtac tgcgaatacc ctcatttatg taaaaggaca aggcatcgta
    12541 ttgaatgagc cttccgtggt ggccattcgt caggatcgtg ccggttcacc gaaaagcgta
    12601 gctgcagtag gtcatgacgc gaagcagatg ctgggccgta cgccgggcaa tattgctgcc
    12661 attcgcccaa tgaaagacgg cgttatcgcc gacttcttcg tgactgaaaa aatgctccag
    12721 cacttcatca aacaagtgca cagcaacagc tttatgcgtc caagcccgcg cgttctggtt
    12781 tgtgtaccgg ttggcgcgac ccaggttgaa cgccgcgcaa ttcgtgaatc cgcgcagggc
    12841 gctggtgccc gtgaagtctt cctgattgaa gaaccgatgg ctgccgcaat tggtgctggc
    12901 ctgccggttt ctgaagcgac cggttctatg gtggttgata tcggtggtgg taccactgaa
    12961 gttgctgtta tctccttgaa cggcgtggtt tactcctctt ccgtgcgcat tggtggtgat
    13021 cgtttcgacg aagctatcat caactatgtg cgtcgtaatt acggttctct gatcggtgaa
    13081 gccaccgcag aacgtatcaa gcacgaaatc ggttcggctt acccgggcga tgaagtccgt
    13141 gaaatcgaag ttcgtggccg taacctggca gaaggtgttc cacgcggttt taccctgaac
    13201 tccaacgaaa tcctcgaagc actgcaggaa ccgctgaccg gtattgtgag cgcggtaatg
    13261 gttgcactgg aacagtgccc gccggaactg gcttccgaca tctccgagcg cggcatggtg
    13321 ctcaccggtg gtggcgcact gctgcgtaac cttgaccgtt tgttaatgga agaaaccggc
    13381 attccagtag ttgttgctga agacccgctg acctgtgtgg cgcgcggtgg cggcaaagcg
    13441 ctggaaatga tcgacatgca cggcggcgac ctgttcagcg aagagtaatc ggatgcaggc
    13501 aggggaaatg tctgtttacc ctgcctggtc tgatacgaga atacgcataa cttatgaagc
    13561 caatttttag ccgtggcccg tcgctacaga ttcgccttat tctggcggtg ctggtggcgc
    13621 tcggcattat tattgccgac agccgcctgg ggacgttcag tcaaatccgt acttatatgg
    13681 ataccgccgt cagtcctttc tactttgttt ccaatgctcc tcgtgaattg ctggatggcg
    13741 tatcgcagac gctggcctcg cgtgaccaac tagaacttga aaaccgggcg ttacgtcagg
    13801 aactgttgct gaaaaacagt gaactgctga tgcttggaca atacaaacag gagaacgcgc
    13861 gtctgcgcga gctgctgggt tccccgctgc gtcaggatga gcagaaaatg gtgactcagg
    13921 ttatctccac ggttaacgat ccttatagcg atcaagttgt tatcgataaa ggtagcgtta
    13981 atggcgttta tgaaggccag ccggtcatca gcgacaaagg tgttgttggt caggtggtgg
    14041 ccgtcgctaa actgaccagt cgcgtgctgc tgatttgtga tgcgacccac gcgctgccaa
    14101 tccaggtgct gcgcaacgat atccgcgtaa ttgcagccgg taacggttgt acggatgatt
    14161 tgcagcttga gcatctgcca gcgaatacgg atattcgtgt tggtgatgtg ctggtgacct
    14221 ccggtctggg cggtcgtttc ccggaaggct atccggtcgc ggttgtctct tccgtaaaac
    14281 tcgataccca gcgcgcttat actgtgattc aggcgcgtcc gactgcagga ctgcaacgtt
    14341 tgcgttatct gctgctgctg tggggcgcgg atcgtaacgg cgctaacccg atgacgccgg
    14401 aagaggtgca tcgtgttgct aatgaacgtc tgatgcagat gatgccgcag gtattgcctt
    14461 cgccagacgc gatggggcca aagttacctg aaccggcaac ggggatcgct cagccgactc
    14521 cgcagcaacc gacgacagga aatgcagcta ctgcgcctgc tgcgcccaca cagcctgctg
    14581 ctaatcgctc tccacaaagg gctacgccgc cgcaaagtgg tgctcaaccg cctgcgcgtg
    14641 cgccgggagg gcaatagtgg cgagctatcg tagccaggga cgttgggtaa tctggctctc
    14701 tttcctcatt gcgctgttgc tgcaaatcat gccctggccg gataacctga ttgttttccg
    14761 gccaaactgg gtgttactca tcttgttgta ttggatcctg gccttgcctc atcgcgtaaa
    14821 tgtgggcaca ggttttgtga tgggtgccat actggatctg atcagcggct cgacgcttgg
    14881 cgtacgcgta ttggcgatga gcatcattgc ttacctggtg gcgctgaaat accagctttt
    14941 ccgcaacctc gcattatggc agcaggcgct ggtcgtcatg ttgctttcgc tggtggtgga
    15001 tattattgtt ttctgggcag agtttttagt gattaacgtc tctttcagac cggaagtgtt
    15061 ctggagtagt gtagtcaatg gggtgctctg gccgtggatt ttcttgctga tgcgtaaagt
    15121 ccgtcagcag tttgcagtgc aataaaggtt tctatgactt ctctgtattt agcttccggt
    15181 tctccgcgtc gtcaggagtt acttgcgcaa cttggtgtga cctttgaacg tattgttacg
    15241 ggcattgagg agcagcgtca gccgcaggag agcgcgcagc agtatgttgt gcgtctggcg
    15301 cgcgagaaag cacaggcggg tgtcgcgcaa acggcgcagg atctcccggt gctgggtgcg
    15361 gatactatcg ttatcctgaa tggagaagtg ctggagaaac cgcgcgacgc agagcatgcg
    15421 gcgcagatgt tgcgcaaatt atcgggtcag acccatcagg tgatgacggc agtggcgttg
    15481 gccgacagcc agcacattct cgattgcctg gtggtcaccg atgtgacttt cagaacgtta
    15541 acagacgaag acatcgcggg ctatgtcgcc agcggtgaac cgttagataa agcaggtgca
    15601 tacggtattc aggggctggg tggctgtttt gtcaggaaga taaatggcag ctatcacgcc
    15661 gtagtcggct tacctctggt tgaaacgtat gaattattaa gtaattttaa cgcactgcgt
    15721 gagaaaaggg ataaacatga cggctgaatt gttagtaaac gtaacgcctt cggaaacgcg
    15781 agtggcgtat attgatggcg gtattctgca ggaaattcat attgaacgtg aggcgcgacg
    15841 cggaatagta ggcaatatct acaagggtcg tgtaagtcgt gtacttccgg gtatgcaggc
    15901 ggcttttgta gatattgggc tggataaagc cgcgtttctt catgcatccg atatcatgcc
    15961 gcacaccgaa tgtgtggcgg gtgaagaaca aaagcaattc acggtgcgcg acatctcgga
    16021 actggttcgt caggggcaag atctgatggt gcaggtggtg aaagatccgc ttggcactaa
    16081 aggtgcgcgc ctgaccaccg atatcacgct gccttctcgc tatctggtct ttatgccagg
    16141 ggcttctcac gttggggttt cccaacgtat tgaaagcgaa tcagaacgtg aacgcctgaa
    16201 aaaagtggtc gcagagtatt gcgacgagca gggcgggttt atcatccgta ccgcagcgga
    16261 aggggttggc gaggctgaac tggcctccga tgccgcttat ctgaaacgcg tctggaccaa
    16321 agttatggag cgtaaaaagc gcccgcagac ccgttatcag ctgtacggcg aactggcgct
    16381 ggctcagcgt gttctgcgtg atttcgccga cgccgaactg gaccgcattc gcgttgactc
    16441 acgcctgact tacgaagcat tacttgagtt cacctcggag tacattcccg agatgaccag
    16501 caagctggag cattacaccg gacgccagcc gattttcgat ctctttgatg tcgaaaacga
    16561 aatccagcga gcgctggaac gcaaagtaga actgaaatcc ggtggttatc tcattatcga
    16621 ccagaccgaa gcgatgacca ccgtggacat caataccgga gcgtttgtcg gtcatcgcaa
    16681 tctggacgac accattttca ataccaatat tgaagcgacg caggctatcg ctcgccagtt
    16741 acggttgcgt aatctgggcg ggattatcat tattgatttc atcgatatga ataatgaaga
    16801 tcaccgccgc cgcgtgctgc actcgctgga gcaggcgttg agcaaagacc gggtgaaaac
    16861 cagcgttaat ggtttttcgg cgctggggct ggtggagatg acgcgtaaac gcacccgcga
    16921 aagcattgag cacgtactgt gtaacgaatg cccaacctgc cacggtcgcg gaacggtgaa
    16981 aaccgtggaa acggtatgct atgaaatcat gcgcgagatt gttcgtgtcc accatgctta
    17041 cgactccgac cgtttcctgg tctatgcttc tccggcagta gccgaagcct tgaaaggcga
    17101 agagtcacac tcgctggcgg aagtggaaat tttcgttggc aaacaggtta aagtacaaat
    17161 tgaaccgctc tataaccagg agcagtttga cgtcgtaatg atgtaaacag atgctgggcc
    17221 gccatccggc aaagggtttt tgagtcacat ttttagcaga caaggagtga cgggtgaggc
    17281 gattgccggg gattttactg cttactggag ccgcgctcgt tgtgatcgct gccctgctgg
    17341 ttagcggcct gcgtattgct ttaccgcatc ttgacgcctg gcgtccggaa atcctcaaca
    17401 aaatagaatc cgcgactggc atgccggtag aagccagtca gctctcagcc agctggcaga
    17461 attttggccc gacgcttgaa gcacacgaca tccgtgcaga actaaaagat ggcggcgaat
    17521 tttcggttaa acgcgttact ctggcgctgg atgtctggca gagcctgtta catatgcgct
    17581 ggcagtttcg cgacctcact ttctggcagc tgcgctttcg caccaacact cctatcacca
    17641 gcggtggtag cgatgacagt ctggaagcca gtcacatcag cgatctgttt cttcgtcaat
    17701 ttgaccattt cgatctccgc gacagtgaag tcagtttcct gacgccatcc ggtcagcgtg
    17761 ccgagctggc gatcccacaa ctcacctggc tgaacgatcc acgtcgacac cgtgcggaag
    17821 gcctggtaag cctctccagc cttaccggac agcacggcgt gatgcaggtg cgcatggatt
    17881 tgcgcgatga tgaggggttg ttaagcaatg gtcgcgtctg gctacaggcg gatgacatcg
    17941 acctgaagcc gtggctcggt aaatggatgc aggacaatat tgctctggaa acggcacagt
    18001 tctcccttga aggctggatg acgatcgaca aaggcgatgt aaccggcggt gacgtctggc
    18061 tgaaacaggg cggtgccagc tggttgggcg agaagcaaac gcatacgctg tcggtggata
    18121 atctgaccgc gcatattacg cgtgaaaatc cgggctggca gttctctatt cccgatacac
    18181 ggatcacgat ggacggcaaa ccctggccga gcggagcatt gacgctggcc tggataccgg
    18241 aacaggacgt tggcggcaaa gacaataaac gcagtgacga actccggatt cgcgccagta
    18301 atctggagct ggcaggcctg gagggcatac gcccgctggc cgcgaaactt tcacctgcac
    18361 tgggtgatgt ttggcgctcc acacaaccga gcggcaagat taacactctg gcgctggata
    18421 tcccgcttca ggcggcagac aagacccgtt ttcaggcatc gtggagcgat ctggcctgga
    18481 agcaatggaa attattaccg ggtgcggaac acttctccgg gacgctttcc ggcagcgttg
    18541 aaaatggttt gcttaccgcg tcgatgaagc aggcaaagat gccttacgaa acggtattcc
    18601 gtgcgccact ggaaatcgcc gacggccagg caactataag ctggctgaac aatgacaaag
    18661 gtttccagct ggatgggcgt aatattgacg ttaaagccaa agccgtccat gcgcgcggcg
    18721 gttttcgtta cctgcaacct gctaacgatg aaccctggct gggtattctg gctggcatca
    18781 gtaccgatga tggttcacaa gcctggcact atttcccgga aaacttgatg ggtaaagacc
    18841 tggttgatta cttaagtggc gcgattcagg gcggtgaagc ggataacgcg acgctggttt
    18901 atggtggcaa tccgcaactc ttcccctata aacacaacga aggtcagttt gaagtgctgg
    18961 tgccgctgcg caacgcgaag tttgccttcc agccggactg gcctgcatta actaaccttg
    19021 atattgaact ggactttatt aacgacggtt tatggatgaa aaccgatggc gttaatctgg
    19081 gcggcgtgcg cgcgagtaat ctcaccgcag tgatccctga ctactcgaaa gaaaaactgc
    19141 tgattgacgc tgacattaaa ggtccgggta aagccgttgg cccttacttt gatgagacac
    19201 cgctgaaaga ttctctgggt gcgaccctgc aagaactcca gctcgacggc gatgtgaatg
    19261 ctcgcttaca tcttgatatc ccgctgaacg gcgagctggt aaccgcgaaa ggtgaagtga
    19321 cgctgcgtaa taacagtctg tttatcaaac cactcgacag caccctgaaa aatttgagcg
    19381 gtaaattcag ctttatcaat ggcgatctgc aaagtgaacc actgacagca agctggttta
    19441 atcagccgtt gaacgtggat ttttccacca aagaaggggc aaaagcctac caggttgcgg
    19501 tgaatctcaa cggtaactgg caaccggcga aaaccggcgt tctgcctgaa gcggtgaacg
    19561 aagcattgag tggcagcgtg gcgtgggatg gtaaagtggg cattgatctg ccttatcatg
    19621 ctggcgcgac ctataacgta gagctgaacg gcgatctgaa taatgtgagt agtcacttac
    19681 cttcaccgtt agccaaacct gcgggtgaac cactagcggt aaacgttaag gttgatggca
    19741 atctcaacag ctttgaatta accggacagg ctggtgcgga taaccatttc aatagccgct
    19801 ggttgcttgg tcaaaagctg acgctcgatc gtgctatttg ggcggcagac agtaaaacgc
    19861 tcccgccgtt gccggaacaa agtggcgttg aactcaatat gccgccgatg aatggtgccg
    19921 agtggctggc cctgttccag aaaggcgctg cggagagtgt cggtggtgca gcgagtttcc
    19981 cacaatacat aacgttacgt acgcctatgt tgtcactggg aaatcagcaa tggaataacc
    20041 tgagtattgt ttcgcaaccg acggcaaatg gcaccctggt tgaggcgcaa gggcgtgaaa
    20101 tcaacgccac gctagcgatg cgtaataacg cgccgtggct ggcgaatatc aaatatcttt
    20161 attacaaccc gagcgtggcg aaaactcgtg gtgattcaac gccgtcatca cctttcccga
    20221 caacggagcg cattaacttc cgtggctggc cggacgcaca aatacgatgc acagagtgct
    20281 ggttctgggg gcaaaaattc ggtcgcattg acagtgatat caccatttct ggcgatacgt
    20341 taacgctgac caatggactg attgatactg gtttctcgcg gcttactgcc gatggtgaat
    20401 gggttaataa tccggggaat gaacgtacct cgctgaaagg aaaactgcgc gggcagaaaa
    20461 ttgatgccgc cgcagaattt tttggtgtca cgacgcccat acgccagtcg tcatttaatg
    20521 tggattacga tttacactgg cgcaaagcac cctggcagcc agatgaagcg acgttgaatg
    20581 gcatcattca tactcaactg ggtaaaggcg aaattaccga aatcaatacc ggacatgccg
    20641 ggcaattgct gcgcttattg agcgtagatg ccctgatgcg taagctgcgt tttgatttca
    20701 gagacacttt tggcgaaggg ttctattttg actccattcg cagcaccgcg tggattaaag
    20761 acggggttat gcacaccgac gacacgctgg tggatggcct ggaggcggat atcgccatga
    20821 aagggtcggt aaatctggta cgtcgcgacc tgaatatgga agcggttgtc gcaccagaga
    20881 tttctgcgac ggtgggcgtg gctgcggctt ttgcggttaa ccccattgtt ggcgcggcag
    20941 tttttgccgc tagtaaagtg ctggggccgc tgtggagcaa agtctccatt ttgcgctatc
    21001 acatttcggg tccgctggac gatccgcaaa tcaacgaagt gttgcgccaa ccgcgtaaag
    21061 aaaaagcgca atgatttgac gagggcgcgt aattgcccca atctcatatg ataatcgttg
    21121 ccaaaggcca acgagccaga acataaccgt aggccggata gggcgttcat gccgcatccg
    21181 gcagccgtaa aaaatcctct actgcagtaa ctaacgagta gcaaaaacga tgagtctgaa
    21241 cctggtaagt gaacaattgc tagcggcgaa cagcctgaaa catcaggact tgttcgcgat
    21301 cctcggtcaa ctggccgaac gtcgccttga ttatggcgat ctctattttc agtcgagcta
    21361 tcacgaatcc tgggttttag aagaccgcat tattaaagat ggttcttaca acatcgatca
    21421 gggcgttggc gtgcgtgcaa tcagcggtga aaaaaccgga tttgcttacg ctgaccaaat
    21481 cagcctgctg gcgctggaac agagtgcgca agcggcgcgc accatcgtcc gtgatagtgg
    21541 tgatggcaaa gtacagacgc tgggcgcggt agagcatagc ccgttgtata cctcggtaga
    21601 tccgctgcaa agcatgagcc gtgaagagaa gctggatatc ctgcgtcgcg tcgataaggt
    21661 tgctcgcgaa gcggacaagc gcgtacagga agtgactgcc agcctcagtg gtgtctatga
    21721 attaattctg gttgcggcca ccgacggcac gctagcggcg gatgtccgtc cgctggtgcg
    21781 tctttccgtg agcgttctgg tcgaagaaga tggcaaacgc gaacgcggtg ccagtggcgg
    21841 cggcggtcgt tttggttatg agttcttcct tgccgatctt gacggcgaag tccgtgcgga
    21901 tgcatgggca aaagaagcag tgcgtatggc gctggtcaat ctttctgccg ttgctgcacc
    21961 agcgggcacc atgccggtag tacttggcgc aggttggccg ggcgtgctgt tgcatgaagc
    22021 ggtaggtcac ggtctggaag gcgacttcaa ccgccgcggc acttcagtat ttagtgggca
    22081 ggtcggggag ctggtggctt cagaactgtg taccgtggtt gatgacggca cgatggtcga
    22141 tcgccgtggt tcggtggcga ttgatgacga aggtacgcca ggccagtaca acgtgctgat
    22201 tgagaacggc attctgaaag gctacatgca ggataaactc aacgcgcgtt tgatggggat
    22261 gacgccgact ggcaacggtc gccgtgaatc ctacgcccat ctgcccatgc cgcgtatgac
    22321 taacacctat atgctgccgg gtaaatcgac cccgcaggaa attattgaat ccgttgagta
    22381 cggtatctat gcaccgaact ttggtggcgg tcaggtggat atcacctccg gcaaattcgt
    22441 tttctccact tcagaagcat atctgattga aaacggtaaa gtaacgaagc cggtgaaagg
    22501 cgcaacgttg attggttccg gtatcgaaac catgcagcag atttcgatgg ttggcaacga
    22561 cctgaaactg gataacggcg tgggtgtctg cggtaaagaa gggcaaagtt tgccggttgg
    22621 cgtgggccag ccaacgctga aagttgataa cctgactgtt ggcggtactg cgtaataatt
    22681 tcattatttt cagaaggata attaatcttt ctacgtgcac gaacggtccc ctcgctcctc
    22741 tggggttagg gtgaggggaa cccgttggca caggtttgta gaacgtaaca gtacaatatg
    22801 aattacttct ctttcccgcg cccgtgcatc tcctgaaaca atttaccgac ctcaacaaaa
    22861 taatccgtca gcgagttgat cacgacctgt accttcagcg gcagcttatc tttttcggta
    22921 tataacgcat aaaccgggcg tggatctgac tgataacgcg gcagcaggat ctccagctca
    22981 ccgcgattga tctcgttgat cacccacatc agcggcacgt aggcgatccc ggcacccgcc
    23041 gtcagccagc gcaccagcgt catcggatca ttagtcacaa atcttccttg tgggatcagg
    23101 cgagtcgaga tcccttccgg tgcgatcagt tcaaattcat tgtcgggccg cacgctgtat
    23161 tcaagccatg aatgactact caaatcggcg ggtttttccg gtatgccgta ttgtgtgaga
    23221 tagcttttcg cggcgcacac caccatcggc atcgcgccca gacggcggga aaacaggctg
    23281 gaatcctgca acgcgccgac gcggatcacc acatccagac cgtcggcaat caggtcgggg
    23341 gctggaattc cggtaaccag attgacgctc aaacctgggt attctttcag catttttgct
    23401 gtcagcccgg cgagaacatt ttgtgccata gttgaagaac agccaatgcg cagcgtcccg
    23461 atgggggtgt tattgaaggc atacagttgc tcatgaacat cctgcacttc atgaagcatc
    23521 cgacggcagc cctggtagta aattctgccg gcttcggtca ggccaatgct gcgtgtgcta
    23581 cggtttaaca gctttacctg caactcatct tccagttttg acaccgtctg actgatggac
    23641 gaaacgctca tctgtagctg tctggcggcg gcggtaaaag agccaaattc aactactttg
    23701 gcaaacaccg acatgcgttt tagtcgttcc attattcact ctgacttaaa agtgatttag
    23761 atcacataat atagataaca gcataacagt tacgctaata tattaaatat caatctacag
    23821 caatgttgct ctcgcccggc tttccatgcc cttctctgcg gcgacagatg ctgaaaataa
    23881 taacgcctgc tctctcttta caaccaaggt caacatgagt ctgtttcccg ttatcgtggt
    23941 gtttggactg tccttcccac cgatattttt tgaattgctt ttatcactgg cgattttctg
    24001 gctggtgcgc cgggtacttg tgccaacagg tatctacgac tttgtctggc atccggcgtt
    24061 gttcaacacc gcgctctatt gctgcttgtt ttatttgata tcgcgactgt tcgtttgagg
    24121 ttgaagtgaa aacactaata agaaaattct cccgtacggc catcacggtc gtattagtca
    24181 ttctagcctt catcgcaatt tttaatgcct gggtctatta caccgaatcc ccctggacgc
    24241 gtgacgcgcg ctttagcgct gacgtcgttg cgatcgcgcc ggacgtttct ggactcatta
    24301 cccaggtgaa tgttcatgat aaccagctgg tgaaaaaagg acagatactg ttcaccatcg
    24361 accagccgcg ctatcaaaag gcgcttgagg aagcgcaagc cgatgttgct tattatcagg
    24421 tactggcaca ggagaaacgc caggaggccg gacgtcgtaa ccgtctcggt gtgcaggcga
    24481 tgtctcgcga agagatcgac caggccaaca acgtactaca aacggttctg catcagttag
    24541 cgaaagcgca ggcgacccgc gatctggcaa aactggatct tgaacgcacg gtgatccgcg
    24601 cgccagcaga tggctgggtg accaacctca acgtctatac cggtgagttt attactcgag
    24661 gatcaacggc ggttgcgctg gtgaaacaga actccttcta tgtactggcc tatatggaag
    24721 aaactaagct ggaagggggg cgtccggggt atcgtgcaga gatcacgccg cttggcagta
    24781 acaaagtgct gaaagggact gttgatagtg ttgccgcagg ggtcaccaac gccagcagca
    24841 cgcgtgacga caaagggatg gcgactatag actctaatct tgaatgggtg cgtcttgcgc
    24901 aacgtgttcc ggttcgtatt cgtctcgaca accagcaaga gaacatctgg cctgcgggca
    24961 ccactgctac agtggtggtc actggcaaac aagatcgcga cgaaagccag gattcgttct
    25021 tccgtaaaat ggcccatcgc ctgcgtgagt ttggttaatc acgatgggta ttttctccat
    25081 tgctaaccaa catattcgct ttgcggtaaa actggcgacc gccattgtac tggcgctgtt
    25141 tgttggcttt cacttccagc tggaaacgcc acgctgggcg gtactgacag cggcgattgt
    25201 tgccgccggt ccggcctttg ctgcgggagg tgaaccgtat tctggcgcta ttcgctatcg
    25261 tggctttttg cgcatcatcg gcacatttat tggctgtatt gccggactgg tgatcatcat
    25321 tgcgatgatc cgcgcaccat tattgatgat tctggtgtgc tgtatctggg ccggtttttg
    25381 tacctggata tcctcgctgg tacgaataga aaactcgtac gcgtgggggc tggccggtta
    25441 taccgcgctg atcattgtga tcaccattca gccggaacca ttgcttacgc cgcagtttgc
    25501 cgtcgaacgt tgtagcgaga tcgttatcgg tattgtgtgt gcgattatgg cggatttgct
    25561 cttttctccg cgatcgatca aacaagaagt agatcgagag ctggaaagtt tgctggtcgc
    25621 gcaatatcaa ttaatgcaac tctgtatcaa gcatggcgat ggtgaagttg tcgataaagc
    25681 ctggggcgac ctggtgcgac gcaccacggc actacaaggc atgcgcagca acctgaatat
    25741 ggaatcttcc cgctgggcgc gggccaatcg acgtttaaaa gcgatcaata cgctatcgct
    25801 gacgctgatt acccaatcct gcgaaactta tcttattcag aatacgcgcc cggaattgat
    25861 cactgatact ttccgcgaat tttttgacac gccggtagaa accgcgcagg acgtccacaa
    25921 gcagctcaaa cgcctgcgga gagttatcgc ctggaccggg gaacgggaaa cgcctgtcac
    25981 catttatagc tgggtcgcgg cggcaacgcg ttatcagctt ctcaagcgcg gcgttatcag
    26041 taacacaaaa atcaacgcca ccgaagaaga gatcctgcaa ggcgaaccgg aagtaaaagt
    26101 agagtcagcc gaacgtcatc atgcaatggt taacttctgg cgaaccacac tttcctgcat
    26161 tctgggcacg cttttctggc tgtggacggg ctggacttcc ggcagtggtg caatggtgat
    26221 gattgcggtc gtgacgtcac tggcaatgcg tttgccgaat ccacgcatgg tggcgatcga
    26281 ctttatctat gggacgctgg ccgcgctgcc gttagggctg ctctactttt tggtgattat
    26341 ccctaatacc caacagagca tgttgctgct gtgtattagc ctggcagtgc tgggattctt
    26401 cctcggtata gaagtacaga aacggcgact gggctcgatg ggggcactgg ccagcaccat
    26461 aaatattatc gtgctggata acccgatgac tttccatttc agtcagtttc tcgacagcgc
    26521 attagggcaa atcgtcggct gtgtgctcgc gttcaccgtt attttgctgg tgcgggataa
    26581 atcgcgcgac aggactggac gtgtactgct taatcagttt gtttttgccg ctgtttccgc
    26641 gatgactacc aatgtggcac gtcgtaaaga gaaccacctc ccggcacttt atcagcagct
    26701 gtttttgctg atgaataagt tcccagggga tttgccgaaa tttcgcctgg cgctgacgat
    26761 gattatcgcg caccagcgcc tgcgtgatgc gccgatcccg gttaacgagg atttatcggc
    26821 gtttcaccga caaatgcgcc gcacagcaga ccatgtgata tctgcccgta gcgatgataa
    26881 acgtcgtcgg tactttggcc agttgctgga agaactggaa atctaccagg aaaagctacg
    26941 catctggcaa gcgccaccgc aggtgacgga accggttcat cggctggcgg ggatgctcca
    27001 taagtatcaa catgcgttga ccgatagtta agtcaaaacc gacgccaaaa gcgtcggttt
    27061 tttcatggct atacttagcg atgcacggca gaactcgccg cgaaacgtga cggtggcaac
    27121 agatgaatat ttataccttt gattttgatg agattgagag tcaggaggat ttttatcgtg
    27181 actttagcca aacctttggt ctggctaaag ataaggtacg cgatctcgac tcactatggg
    27241 atgtgttaat gaacgatgtc ctgccgctac cacttgagat tgaatttgtt catctgggag
    27301 agaaaacgcg tcgccgtttt ggcgcgttaa tattgctgtt tgatgaggca gaggaagagc
    27361 tggaagggca tttgcgtttt aatgttcgtc attagcgcaa aaaaaagccc ccgaaccggg
    27421 ggcaatatcg tcggacaaga cgatgagggt ttatttgtac agttcagccg tagcgtgcca
    27481 ggtgtcaccg ctacgagctt cagtaatctg gtaggccgtt gcgcctttct cttccgcttt
    27541 tttgttcagc atttcacgca tatccattgg cgaagacgcc acaccactta cggatacggt
    27601 cccgattgct tcacgatttt gtgcttgtgc agtatcaatg gagtcggcag cgaatgcacc
    27661 gaaagagaga acagaaagta cgcttaatgc agctacagtg gttttgattt tcatgatttt
    27721 tacctcgaca taatctttta gctgggcctt tgtttcgtga cccatatcac aaaatcaagc
    27781 atacactaat tattgaacta attaatacca cgctaactat ttcagttggt actaagtgtt
    27841 aaaaatgtga att
//
LOCUS       DAZRUF010000027        27146 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZRUF010000027 DAZRUF010000000
VERSION     DAZRUF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27146
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.027"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..412
                     /locus_tag="ACHXOL_000949"
     CDS             <1..412
                     /locus_tag="ACHXOL_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR6808000.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(460..1332)
                     /locus_tag="ACHXOL_000950"
     CDS             complement(460..1332)
                     /locus_tag="ACHXOL_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR6808001.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(1344..2438)
                     /locus_tag="ACHXOL_000951"
     CDS             complement(1344..2438)
                     /locus_tag="ACHXOL_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR6808002.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHCATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(2471..3469)
                     /locus_tag="ACHXOL_000952"
     CDS             complement(2471..3469)
                     /locus_tag="ACHXOL_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR6808003.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(3494..5005)
                     /locus_tag="ACHXOL_000953"
     CDS             complement(3494..5005)
                     /locus_tag="ACHXOL_000953"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR6808004.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(5028..6011)
                     /locus_tag="ACHXOL_000954"
     CDS             complement(5028..6011)
                     /locus_tag="ACHXOL_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR6808005.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(6108..9385)
                     /locus_tag="ACHXOL_000955"
                     /pseudo
     CDS             complement(6108..9385)
                     /locus_tag="ACHXOL_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            9503..10696
                     /locus_tag="ACHXOL_000956"
                     /pseudo
     CDS             9503..10696
                     /locus_tag="ACHXOL_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(10894..12147)
                     /gene="glyA"
                     /locus_tag="ACHXOL_000957"
     CDS             complement(10894..12147)
                     /gene="glyA"
                     /locus_tag="ACHXOL_000957"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR6808006.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            12475..13665
                     /gene="hmpA"
                     /locus_tag="ACHXOL_000958"
     CDS             12475..13665
                     /gene="hmpA"
                     /locus_tag="ACHXOL_000958"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR6808007.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(13710..14048)
                     /gene="glnB"
                     /locus_tag="ACHXOL_000959"
     CDS             complement(13710..14048)
                     /gene="glnB"
                     /locus_tag="ACHXOL_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR6808008.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(14109..15443)
                     /gene="glrR"
                     /locus_tag="ACHXOL_000960"
     CDS             complement(14109..15443)
                     /gene="glrR"
                     /locus_tag="ACHXOL_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR6808009.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(15433..16146)
                     /gene="qseG"
                     /locus_tag="ACHXOL_000961"
     CDS             complement(15433..16146)
                     /gene="qseG"
                     /locus_tag="ACHXOL_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR6808010.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLEKLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(16311..17738)
                     /gene="qseE"
                     /locus_tag="ACHXOL_000962"
     CDS             complement(16311..17738)
                     /gene="qseE"
                     /locus_tag="ACHXOL_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR6808011.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(18296..22183)
                     /gene="purL"
                     /locus_tag="ACHXOL_000963"
                     /gene_synonym="purI"
     CDS             complement(18296..22183)
                     /gene="purL"
                     /locus_tag="ACHXOL_000963"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR6808012.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            22441..23997
                     /gene="mltF"
                     /locus_tag="ACHXOL_000964"
     CDS             22441..23997
                     /gene="mltF"
                     /locus_tag="ACHXOL_000964"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR6808013.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALNITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDCTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(23994..24497)
                     /gene="tadA"
                     /locus_tag="ACHXOL_000965"
     CDS             complement(23994..24497)
                     /gene="tadA"
                     /locus_tag="ACHXOL_000965"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR6808014.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(24555..25190)
                     /gene="yfhb"
                     /locus_tag="ACHXOL_000966"
     CDS             complement(24555..25190)
                     /gene="yfhb"
                     /locus_tag="ACHXOL_000966"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR6808015.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            25399..26247
                     /locus_tag="ACHXOL_000967"
     CDS             25399..26247
                     /locus_tag="ACHXOL_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR6808016.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(26586..27143)
                     /gene="pinE"
                     /locus_tag="ACHXOL_000968"
                     /pseudo
     CDS             complement(26586..27143)
                     /gene="pinE"
                     /locus_tag="ACHXOL_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT         6478 a         7469 c         6835 g         6364 t
ORIGIN      
        1 acgttatttt gattttggag ctgcccgccc cgttttgtta ttaattgccc gtatcgccgt
       61 ggtcttaatt ttcattattt ttggttttcc caaaatgatg ggctttgacg gtacggtcca
      121 atatatggcc tcgttgggcg cgccaatgcc gatgctggca gcgattattg cggtagttat
      181 ggaagtgccc gccgcgatat taatcgtgct tggctttttc acccgtccgc tggcggtgct
      241 gtttattttc tacacgctgg gtacggcggt gattggtcac cattactggg atatgaccgg
      301 cgatgcggtt gggccaaata tgattaattt ctggaagaat gtcagtatcg ctggcgcgtt
      361 cttgctattg gcaattaccg ggccgggggc aatttctctc gatcggcgtt aggaaagatg
      421 ccggatgcgg cgtgaacgcc ttatccggca tttaataaat tacagccact ctacccgcaa
      481 cgacatctct gaagttgttg attcccctgg tgctagcgca atgagatcac ccccttccgg
      541 gcgatgatga tcatccggcg catggctcat cggttccaga cagaagaaat caaacgcata
      601 tcctttatca aacgcagggt cggaaacaaa gatgaaataa cacggtgcag gtggcgtcgt
      661 ttccatgatg atggcataac cttcctgcgg ctgctcgatg cgggcctgac cattccatcc
      721 ggcaaaacca ttgttcaccc actggcgcgg caacggcgcg agctggttaa aatccagttc
      781 ctgcggtagc tgctcgcaaa actcacccgc cagccactgc tcccgttcca gccagtaacc
      841 gctcgcctgc gcctgaatcc gcgtttgtgg tgacaacgga aaataaggat gccagccggt
      901 accaaatggc agcgtctctg ccccttgatt ggtgacagag agcgtcaccg tcagcgtatc
      961 cgccgttaaa tgaaacgcct gacttacccg atagtgatag acaccgctgc gatgttcata
     1021 caccagacac aaactatcat cgctgtgcga gacacattgc cactcgccca gccagccatc
     1081 gccgtgtaga tagtgcgcat cccactcaac attcggttgc agttgatact cacgcccctg
     1141 ccagacaaaa cggttgccgc tcacccggtt tgcaaatggc accagcggaa aacatgaggc
     1201 atcggttgcc acaccgcttt ttttaccagg acgtaagagc ggcgtcgtat cgcgccagaa
     1261 gccttcgatt acgccgccct ggtcagaaac gtccagcttt agcgacccgt gggataaggt
     1321 atagatggtc atccgctaac tccttaatcc gggaagttaa tcacaacttt cccgcatttg
     1381 ccgctcgcca tcagcgcata ggcatcacct gcctgttcca gcgagaagcg atgggtaatg
     1441 gcgttacgcg gccacagctt ccagtcagtc aaatcgtggg cgcatttttc catatggaac
     1501 agactggtca cccaggagcc gataatccgc cgttgatggt gcatcagatc ggcgctgacc
     1561 tcgaattcca cttttccggt ttcaccaatg taaaccaccc gtccccagtc agcggtggat
     1621 tgcagtgcca gcaagcgacc tgcggcatta ccagaacaat cgagcgcaac atccgcgcca
     1681 ccgtgggtga gttcggcgat aatctgcggc agaccttcgg tggttgctaa atagccgtga
     1741 tccatcaccc ctaactgttt tgccatcgcc agacgttccg gcagcatatc aacgccgatg
     1801 atccgttttg caccgcgacc tttcgccagc atcatcgcca tcatgccgac cggccccaga
     1861 ccgaccacca gcacgttatc gctgccggaa acttcgccgc gcaaaatccc ttcataggcc
     1921 gtgccaacgc cgcaactgat aaacgcacca tcttcgtagc tcagcgcatc cggcaggagg
     1981 atcagatctt tttcttccgc cagcagatat tcggcatgac cgccgtcacg ctgccagccg
     2041 taagccgctt ttcctttgcc agtacaagag ataggaaagc cgcggcggca gttcgggcaa
     2101 aaaccacagc cagaaatgtg atacaccagc acgcggtcgc cctctttaaa atggcggcag
     2161 ccttgcccca tcgccacaat ctgcccgcac ggttcatgac cgttgataaa tccctggtat
     2221 aacggtttat cgggtgccgc cgccgtggca cagtgttgat gatagatata gtggacatcg
     2281 cttccgcaaa tcccggagga tttcattttg atcagtacct ggttaatccc cggcgtcggc
     2341 accgcaactt cccgcagatc gacggttgaa tttcctggta aataagctgc cagcatcgtt
     2401 ttcataaatc cctcattaac aatacgatta attttcatcc ctgccgcacc cgcgccaggg
     2461 gcgttaaggt ttagcgtttc gctttactgc ttcgctgggt cagcaagata ttcgccagca
     2521 ccgccaccac gatgatgacg ccgcgtacca cctgctggaa aaaggagtta ataccgagca
     2581 gcaccagacc gttaccgatt agcgtaatca ccagcacacc aagcaatgta ccgaacaggg
     2641 aaccgcgacc gccggaaagt gccgtaccgc cgaccacgac cgcggcgatg acgtcaaact
     2701 ccagaccgtt tgcggcacct gcgttaccgg aaccgaggcg tgccgccagc aaaatgccgg
     2761 tcaccgccgc taataatccc gaaagggtaa agataagaat gcgcacccga cgaacgttaa
     2821 taccgcacaa ctgcgccgcc gtggcattac cgccaacggc aaaaaccgag cgcccgaagg
     2881 cggtttttcg gctaatgaac acaaacagcg caaacaacac catcatgatc agcgcggata
     2941 ctggcacacc gagaaattgt ccgcccagcc agtccagcac ctcgttttcg tcaatcggca
     3001 ctggcagtgc gttggtcata aacagcccca ttccgcgcag ggcgctccac agccccagtg
     3061 tggcaacgaa acttggtacg ttaaacacgc cgcgcagcac cccggcgagc gttcccatca
     3121 gcgcgcctaa cagcaacacc agcagacaag caatcgccaa cggaacttca aattgcagca
     3181 aaaatgccag gcacaccgag acaaaagcca ccatcggccc aacgctgaca tcaatttcac
     3241 cggagataat aatcagcgtc atcgcccagg cggcaatccc aatggtggcg gcatcgcgca
     3301 gcacgttcat ctggttattc aatgagataa agccaggcgc gttcagggag aagaccagat
     3361 aaagaatggc aatcaccacc agcaaaccga tctcattaat atggcgactg acaaattgtt
     3421 tgagcgagac gctcttgccc tgcggcaatg gtaatgacga agcagacatc ttcaggttcc
     3481 tcggtaaaat tcatcagtgc acagacagaa tggcggacat cagctcatcc acattgaccg
     3541 gcgagtgaaa ctcctgcgag aacgtgccgt gctgtaataa caggatgcgg tcacacacca
     3601 gcggcaactc ctccacttca ctggagataa acaccacgct ttttccttcg gcagccagct
     3661 cacggacaat acggtaaatc tgctgtttgg cttcgatatc gacgccgcgc gttggctcgt
     3721 cgagcaacaa aatctggctg gcggcataga cccaacgacc gatcaccact ttttgctgat
     3781 tgccaccaga aagcgtgccg atgggcgttt cgctactagc ggccttgacc gtcatccgct
     3841 gcatcacctc ttcggtcagg cggcggatgg tggaccattg cagcactccg ttggcgctga
     3901 ttttttgccg attggtcagc actgtatttt cgtcaacgcc caaccaggga atgatccccg
     3961 cttctttgcg gttttctggc gtatagccaa tgccgcgttt cagcatgtcg ccgtaatcgg
     4021 ggcgcgtgat tttctcgccg ttgataacaa tttcgccctg ttcatacgtc tccagcccaa
     4081 caatcgcctt cagcaattca ctgcgccctg cccccagcag gccagcaatg ccaagcactt
     4141 cgccgcgacg taacgtaaag ctgatatcct ccagcttggg cttatggcgt aacgcacgga
     4201 cttccagtac ggcctgatcc acaatttcct gaggggcaac cggcgcaata tcaacgtgat
     4261 cacgccctag cattagcgac acaatatgat gcgtggaggt gttttcgagc atcacatcgc
     4321 ccgccacctg accatcgcgc ataacggtgg cacaggaggc aatgcggcgg atttcttcca
     4381 tccggtggct gacataaatc accgccacgc ccagtgccga catctttttc accgcactga
     4441 tcaccagttc aacttctgca ctggcaagcg agctggtggg ttcatcaaga atgaccacgc
     4501 gcggctcgcc cttcatcacc cgtgcaattt ccaccagttg cttttgcgcc gggcttagcg
     4561 ttgaaacaag ttgttcagga ctaacgtcaa cgcccagcgc ctgtaagcaa cgttgggcat
     4621 cctgcgccat ttgcaggtaa tcaatcatgc cgttgcggcg aggccattga ccaaggcaga
     4681 ggttttctgc caccgtcagc ccttccacca gacttaattc ctgataaacc gcacgaaccc
     4741 ccagttcagc ggcacggcga gtcaacgtag cttcgtcacc ttccagtcgc gtctcgccaa
     4801 tccagatatc accgctatcc gggcgttcgc tgccggtaag cattcgaatg agagtcgatt
     4861 tgcccgcgcc gtttttacct aacagcgcac gaacttcgcc tttattgagc gtgaagttaa
     4921 cgttatccag cgcaacgacg ccggggtaac gcttatttcc tgccaccact tttgctaccg
     4981 ggactgcctc tgttgccgtg aacataggga cctctgcgaa tcagcgatta gggcagacca
     5041 tcaacgtgcg ttgccagcca ctgtttacca tcttccgttt tggtatagag atcgattggc
     5101 acctgaatca ctttttcacc gtcggcttgt ttattgataa ccttcaatgt ttgcgcgaaa
     5161 acagcattgc ccattttctt accggaaata tccactaccg ctttcagcac ctgattgttt
     5221 tccagctcct gagcaatttc ggttgtcata tccgaaccga aaacagcaat ttttccggcc
     5281 tgattttgat tacgtaccgc ttttaccgcg ccgagtgtcg caccgcccga ctcccccata
     5341 atggcgttga gatccggcgt ggagataatc agtttttcac caacggaaat cgctttattt
     5401 aaaacagtcc cttcctgatt agcgacaatt tgcgcgccgg gaacgcggga ttttaatact
     5461 tcttcaaatc ctttacgtcg ctgcacacaa acttcaaagg cttcgcaatt gatgacggca
     5521 attttcggct ggtcaatttt attggcaata aaataatcgg cggcagcgtt acccagtttt
     5581 ttaccaaatt ccagtggatc gccgaccaga tacgccgaga catatttatc gacacccttt
     5641 tgattaatac aggtgttgta gcaaatcacc ggaatacccg cttcactggc gcgacgaacg
     5701 gtacggctac tgccattttc agacactgcc gataaaataa tggcatcgac attacgcgcc
     5761 acgagggtat caacaaaggt actttctttc gaaatatcac cctgggcgtt agtttcaatt
     5821 aactgtacct gtactgaaga atcttttgcc gcatcctgaa cgccctgacg cactccagcg
     5881 taatatccca gggtatcaag gtatattgca ccaatggtca tttctttatc cgcagccctg
     5941 gcaaatagtg cgctacctaa tagcgtagcc attaataata gattacgggt tgttctcatt
     6001 tttttaggca tagtgcctcc tgtagagttt ttattaacaa cggcttattc taattatttt
     6061 gtgatgagcg gcagcgcgtg ccgctcaaat attacagtta atagggttta gtgaacgtag
     6121 ttaaaaataa aaggcatcac ggtggtgaat aatgccgctt tcggccaggc cggattaatc
     6181 gccgtcaatt cagcgcgagc agattcatat tgcgcaacct ccccgagtcc cgcggacgcc
     6241 agcatcgcca ccatcaggca tttttcttta tgctgctgtt gtaaatcgcc atacagcgac
     6301 aacaggtcag gttgtgaaac agcaaagaaa tccgcctcaa tactggtttt cgccatctct
     6361 tgcgcccact gtttcatttc actaaacagt tgctgtgcga tttgttgttc gcccagcagt
     6421 cgcagcgcca ttccttgcca gaagagataa tcaaccggct gatcgttgta ataactgtgg
     6481 atgttaatgg tgcgatcgcc ggtcgccgcc agacgtaaac aacgcgtcgc ttcagtttca
     6541 tcgccctgcg cgttggcgca tatcgcctgc cagaaccaga tgtcgttatc agtttgcccc
     6601 ggtaaacggc cttcgcttaa attctccgga taatgcagcg cggcatgaag cagttcgcag
     6661 gcctgctgcg gctgtctggc atcaagatgc tgccaggcgc gtaataactg gttgaggata
     6721 aactgactgg tgaccttccc ttccccgcct tcccacgggt ggaatttgcg cgtggcgaga
     6781 atgtccgccg ctttgtctgc ctgacccgtg agatgccaca aattgagcag ttctgcggtc
     6841 atgtcgtcgc gtttcagcgc aatttccaga ttattttcca gacgcgccag tcgtttctcc
     6901 ggtgtggctc cacttaactt atccagcaaa tcccgttcga aaagcagacg tgcatcctgc
     6961 ggcgcaagct gataagcatt atcaagataa cgcgcggcca gttcataatc gtgttgctta
     7021 ttccacgcat ggatcgccaa cccgcgccag ccatcggcaa actccggcga catctctacg
     7081 caacgttgcc ataaagtaat ggctttgttg tagctgcgct tgttgtagta gaagcaggcc
     7141 agtaaatgac gggcaaacca gcactcttca atactctcca gcgccgccac ttcttccagc
     7201 gtattcggga aacggacaaa ctgcgggaag acatcaatgg cttttgcgac cagttcgccg
     7261 cgttcggctt tcggcagcaa gctggcttgt agataaagcg ataacgtgcg ctgacagtcc
     7321 agtgcgttca gcatctctgt cgccagagtg ggcattcccc agttaatcag ttgcccggcg
     7381 gtcatcaggg cgttaacgtc gcgtccctga cacagaccgc gccactgcgc gagggctgat
     7441 tcgctacgac catcgaacca gttcagccac cacagaatgg cgttcagcgg ataatcacgc
     7501 agcagtttcg cgcgctgcaa acgcgcgttg tcctgacgac cacttaacac cagcagcaga
     7561 ttatgcaggc aaagcacttc ctgattggtt gggcaggcgc gaagactttg ttggcaaaaa
     7621 tccagaccag cgtcgaagtt accattacgc gccgccagtc gtgccaggcc ataatagcca
     7681 ccggctttgc tgttgccgct ccagaccgca cgccagaaat cctcttcggc ttgttgatat
     7741 tgtccctgac gttcgtaagc actggcgcga atcaaacttg cttgtccaca ctgcgggttt
     7801 ttgttcagcg catgtgcgcg tttcagcgcc tgactggcat acgccaccgc ttgcgggaaa
     7861 tctgcgcggt tatattccag catcgccagc gccaggttac agcgataatc cagcgggtcc
     7921 agcgccacgc cgcgcaggta gtaatcgaac ggtgaacggc tggcgtgatg atattgctcc
     7981 agatgctgac cgataaacca ggcttcatct gtactggtaa tgtcttgtgc tgccagtggc
     8041 gctttggcga cgtccggtaa cggcaacgct tgcggctgat gttcctgata actcagtaca
     8101 atattgccgt cggcatcgga aagctcaatg gtcagcctat caggattgat accgtgcaac
     8161 acgccctgga tggcggtcgc aggcatcagt gccacggcat catcaagtaa cgcgttgcat
     8221 ttgccgattt cgtggatcgc caggcggtat ccgttcaacg gagagatggc atacagcccc
     8281 cactcaatcc cctgctcact acgctggagt tttatcaccg catcgcggga ggcattttgc
     8341 accatgccca gagaatgata aggcaggaaa tactgctcaa aacgcttctc ttcgtaagca
     8401 tcaagccagg taaaatcggg ctggttatcg gcaaaaatac cggtcatcag ttcgatatac
     8461 gggccgttat tgtcggtcag actcttatcc cacgcctggc caaattcact gtgtccccaa
     8521 ctccactgtt ttttacctgg cgcaatatgg tggttggcaa cgtgcagcaa accgccatct
     8581 tcatcgtgac accacgcgcc gacaaaatcg tactgcgatt tttcggccat atatgaggtt
     8641 ggaacgggca catttttata gcgagaaatg tccactccag cggagtagtc cactttgtag
     8701 taagtgccgg tagcgatggg gaaagcggag acggcccgtt tgccgtgatc aaacaccgca
     8761 gtcaaatccg gtgggaaaac gctctgatgc ccttcacccc ctttcactgc cgggttggcc
     8821 caccacaaga aatgacgcgg cgtggcgttg ccgttataga cgcggctggc gatttccagc
     8881 gccgcccagg gcgcagggtg aaacctgtca tcacctgtaa accatgcatc ggctccgttt
     8941 cgcctaccca caccgtctgt gcaccgtctt catgggcttc gagggtgaaa tcaacgggca
     9001 taaaggtggt cgggcgatga tgttgcggcc agttaaactc aatcccaccg gaaatccacg
     9061 gccccagcag ccccaccagc gcaggtttaa tgacttcatt gtgataaaca aaatcgcgct
     9121 gtttcacttt atcccacgcg cgatgcaccc gaccgcccag ttccggcagg atcatcactt
     9181 tgatgtagtc gttttccagc cacaccgcct gccaggattt cagggttttc tgctcgctca
     9241 gcgtatcggt cacgccgtag ggataaaccg cgccggacga tccctgataa acgcgatttt
     9301 ccaggaacat gggatgtata tcctgcggcc cggtttcata ggtcgggatc tcaacgcgct
     9361 cttgccacac ttttactgga gtcatggtgc cctcaatatt aacaagacat actgaattaa
     9421 aagatttgtg gcagtgtatt gaacaatctg gcaatgtttt cgcggaataa tcacgcaatt
     9481 aactaaacaa ggtttagtga agatgagagc ctgcattaac aatcaacaga ttcgccacca
     9541 taacaaatgc gtgattctgg aactgctgta ccggcaaaag cgcgccaata aatcaacgct
     9601 ggcccggctg gcgcaaattt cgattccggc agtcagtaat attttgcagg aactggaaag
     9661 cgaaaaacgg gtggtgaata tcgacgatga aagccagacg cgcgggcata gtagcggtac
     9721 atggctgatt gcgccggaag gtgactggac gctgtgcctg aacgtgacgc ccaccagtat
     9781 tgagtgtcag gttgctaatg cttgtttaag tccgaaaggt gaatttgagt atttacagat
     9841 tgatgcaccg acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc atcgccaccg
     9901 taaattgtgg ccggaccgca ccatcaatct ggcgctggca atccacggtc aggttgatcc
     9961 ggtgaccggc gtgtcgcaaa ccatgccgca agcgccgtgg acaacgccgg ttgaggtgaa
    10021 gtatctgctg gaagagaagc tcggcattcg ggtgatggtc gataatgact gcgtgatgct
    10081 ggcgctggcg gagaaatggc aaaataattc gcagaaacgg gatttctgcg tgatcaacgt
    10141 tgattacggc attggctcgt cgttcgtgat taacgagcaa atttatcgcg gcagtttgta
    10201 tggtagcgga cagattggtc acaccatcgt taatccggat ggcgtcgtct gcgactgtgg
    10261 acgttacggc tgcctggaaa ccgtcgcctc gttaagtgca ttaaaaaaac aggcgcgggt
    10321 atggctaaaa tcacaaccgg ttaatactca gcttgatccg gaaaaactga ctacagcgca
    10381 gttaatcgct gcctggtaga gtggagaacc gtggatcacc agttgggttg atcgcagtgc
    10441 caatgccatt ggtttgagtc tgtataactt cctcaacatc ctcaatatta atcagatttg
    10501 gttgtacggt cgcagttgtg cctttggtga gcactggctt aataccatta ttcgccagac
    10561 gggatttaac ccgttcgacc gcgacgaagg gccgagcgtg aaagcaacgc aaattggctt
    10621 tgggcaatta agccgcgcac aacaggtgtt ggggattggc tatttgtatg ttgaggcgca
    10681 gttacgacag atttgatggc gcaataacgt agaaaggctt cccgaaggaa gccttgatga
    10741 tcacatacct aagattgcct gatgcgctac gcttatcagg cctacacgga tattgcaata
    10801 tattgaattt gcaaagtttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
    10861 aacgagcaca ttgacagcaa atcaccgttt cgcttatgcg taaaccggga aacgtgcgca
    10921 gatgtcgaga actttacctt tgatgcgctc gataacggct tcatcattga tgctgtccag
    10981 cacgtcacac atccagccag ccagttcttt cgcttctgct tctttgaagc cgcgacgcgt
    11041 aatcgccgga gtacccacgc gaataccgga ggtcacaaac gggctcttcg gatcgttcgg
    11101 tacgctgttt ttgttgacgg tgatgttagc acggcccaga gcggcgtctg cttctttacc
    11161 ggtcaggttt ttatcaacca gatcaaccag gaacaggtgg ttatcagtgc cgccggaaac
    11221 cactttgtag ccgcgctcaa ggaacacttc taccatcgct ttagcgtttt tcgcgacctg
    11281 ctgctggtaa gttttgaact caggctccat cgcttctttc agagcaaccg ctttaccggc
    11341 gattacgtgc atcaacggac cgccctgacc accagggaaa acggcagagt tcagtttttt
    11401 gtacagctct tcgctaccac ctttcgccag gatcaggccg ccgcgcggac ccgccagggt
    11461 tttgtgagtg gtggtagtaa caacgtgagc atgaggaacc gggttcgggt agacgccagc
    11521 agcaaccagg cccgcaacgt gcgccatatc aacgaacagg taagcaccga tgctgtcagc
    11581 gatttcacgc attttcgccc agtcaaccac gccggaatat gcagagaaac caccgataat
    11641 cattttcggc ttgtgttctt tggcttgttt ttccagatcg gcgtagtcga tatgaccggt
    11701 agcatcgata ccgtaaggaa cgatgttgta cagtttaccg gagaagttaa ccggagaacc
    11761 gtgagtcagg tgaccgccat gcgccaggtt catacccaga acggtatcac ctggttccag
    11821 cagcgcggtg tagaccgcaa agttagcctg ggagccggag tgcggctgga cgttagcgta
    11881 gtcagcgccg aacagttctt tcgcacgatc gatcgccagt tgttcaacga tatcaacata
    11941 ctcgcaaccg ccgtagtagc gtttgcccgg ataaccttca gcatatttgt tggtcagctg
    12001 agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt tcggaggcga tcagttcgat
    12061 gtgctcttcc tgacgtactt tttcctgctc catagcctgc cacagttcgg catcataatc
    12121 ggcaatgttc atttcacgct ttaacatccg catctcctga ctcagctaac aataaaattt
    12181 ttggccttta taggcggtcc tgttggacaa cggcgaacag tataaccgaa tcattgtgcg
    12241 ataacaggtc ttgacaaagg aatttacgca aacgattacc ttcaggctac gcaaggcttt
    12301 ggagaataaa gagcttgcaa ccggaaacgg atttcttttc aggtttgtga tgcaaatttt
    12361 tcacttcatc acattctttc tgaaaaacac caaagaacca tttacattgc agggctattt
    12421 tttataagat gcatttgaga tacatcaatt aagatgcaaa aaaaggaaga ccatatgctt
    12481 gacgctcaaa ccatcgctac agtaaaagcc accatccctt tactggtgga aacggggcca
    12541 aagttaaccg cccatttcta cgaccgtatg tttactcata acccagaact caaagaaatt
    12601 tttaacatga gtaaccagcg taatggcgat caacgtgaag ccctgtttaa cgctattgcc
    12661 gcctacgcca gtaatattga aaacctgcct gcgctgctgc cagcggtaga aaaaatcgcg
    12721 cagaagcaca ccagcttcca gatcaaaccg gaacagtaca acatcgtcgg tgaacacctg
    12781 ttggcaacgc tggacgaaat gttcagcccg gggcaggaag tgctggacgc gtggggtaaa
    12841 gcctatggtg tactggctaa tgtatttatc aatcgcgagg cggaaatcta taacgaaaac
    12901 gccagcaaag ccggtggttg ggaaggtact cgcgatttcc gcattgtggc taaaacaccg
    12961 cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg acggtggcgc agtggcagaa
    13021 taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg aaggtttccc gcatcaggaa
    13081 attcgtcagt actctttgac tcgcaaaccg gatggcaaag gctatcgtat tgcggtgaaa
    13141 cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc acgccaatgt tggcgatgtc
    13201 gtgaaactgg tcgctccggc aggtgatttc tttatggctg tcgcagatga cacaccagtg
    13261 acgttaatct ctgccggtgt tggtcaaacg ccaatgctgg caatgctcga cacgctggca
    13321 aaagcaggcc acacagcaca agtgaactgg ttccatgcgg cagaaaatgg cgatgttcac
    13381 gcctttgccg atgaagttaa ggaactgggg cagtcactgc cgcgctttac cgcgcacacc
    13441 tggcatcgtc agccgagcga agccgatcgc gctaaaggtc agtttgatag cgaaggtctg
    13501 atggatttga gcaaactgga aggtgcgttc agcgatccga caatgcagtt ctatctctgc
    13561 ggcccggttg gcttcatgca gtttaccgcg aaacagttag tggatctggg cgtgaagcag
    13621 gaaaacattc attacgaatg ctttggcccg cataaggtgc tgtaatttga tgttgccgga
    13681 tggaaacatc cggcaaccct tgacgcggtt taaattgccg cgtcgtcctc ttcaccggta
    13741 cggatgcgaa tgacccgtgc cacgtcaaag acgaagattt taccgtcacc gattttgccg
    13801 gtttgcgccg tgcgaataat ggtatcgaca caggtatcga caatgtcgtc cggtacgaca
    13861 atctcaattt tcactttcgg cagaaaatcc accatatact ccgcgccgcg gtacagctcg
    13921 gtatggcctt tctggcgacc aaagcctttc acttcggtca ccgtcatgcc ggtaataccg
    13981 acttcggcca gtgcttcgcg gacatcgtcc agcttgaagg gttttataat cgcatcaatc
    14041 tttttcatgc tattccttga aaaggtcgcc tgtcttttga tctgctaaac gtaacacata
    14101 acgccaattc attccttgaa atcgtttgca tccagctcgt gtcgggaaag cagtttataa
    14161 aattctgtcc ggttgcgccc cgccattctc gccgcgtggg tgacgttgcc tttggtgatt
    14221 tgcagcagct tacgcaaata gttgagttca aactgattac gtgcctcaac aaaggttggc
    14281 agcgccgtat tttcaccctc cagcgcctgc tccaccagcg catcactaat caccggagat
    14341 gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact ggcgcacatt acccggccag
    14401 ctcgcggtca tcaggcgttt catcgcatcg gtagagaacg cgcggacaaa cggtttatgt
    14461 cgctctgccg cctggcgcaa caggtgattt gccagtagcg gaatgtcttc tgtgcgctcc
    14521 gccagtgccg gaattttcag gctgacaacg ttgaggcggt aatagagatc ttcgcggaat
    14581 tccccgcgcg ccatcgcttt tggcagatca cggtgagtgg cagaaataat ccgcacattg
    14641 atatcaatat cgcggttact gcccagcggg cgcactttac gctcctgcaa cacgcgcagc
    14701 agtttgacct gtaacggcgc aggcatatcg ccaatttcat cgagaaatag cgttccgcct
    14761 tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc cagtaaacgc gccacgcgca
    14821 tgaccaaaca gctccgactc cagcaattgc tcgggcaatg cgccacagtt aatagcaata
    14881 aatggtttgc tgttgcgcgg gctggcgttg tggatagcct gggcgaaaat ctctttcccg
    14941 gtgccgctct gaccgttaat caaaacgctg acgtctgatt gcgccaccag ccgcgcctgt
    15001 tccagcaaac gcagcatcag cgggctgcgg gtgacaattg cctcgcgcca gcgttcatcg
    15061 gtggctggcg cggattgctc cagcgcatcg tcaattgcct gatatagcgc atctttatcg
    15121 acaggcttgg tgaggaaact aaaaacgccc tgctgtgttg cagcaacggc atcggggata
    15181 gaaccatgcg cagtaagaat aattactggc attcccggct gcactttctg gatttcagca
    15241 aatagctgca taccgtccat ttcatccatc cgcaggtcgc tgataactaa atctactttt
    15301 tcgcgattca gtacccgtaa tccttcagcg ccactttccg ccgtgaccac actgtagcct
    15361 tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg gatcgtcatc gaccaataat
    15421 aaatgcgcag gtttatggct catcaggagt gacctcatgg gtggatggcg cgggcttttc
    15481 actctcgtgc ggcgtatccg gcgagaaatt tccagtcggt ttgcgggtcg agagctggcg
    15541 ttcaatatcg gtcagttttt ccagcttgcg ggtggtgagt tccagttgct gctgtagaac
    15601 gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg gactgctgga gtttactgta
    15661 acgttggcgc tcttccgcca gttgcagttg cagcgcctga ccgtcgcgcc agagttgata
    15721 cagtgggcga acctgtgccg ggatctcggt acttaacgct tcaatacgcg cgaccagctg
    15781 gcggcgctca tacggcgtaa ttttggcgtc ggcgagcaaa atcccttgtt taaaggtatt
    15841 ttgccagctg ccgtcgtcat attggcgagc ttgctgacgc gactgcgcag gcattaaacg
    15901 atcagcacaa tccatcgccc gcagccagta aagcggattg gtttcggttg atttaccttg
    15961 cagcgcccag atgtcgctac attcagtaga aagatagtca gccagttgat aaaccggaat
    16021 tttttcttct gctggcgtat caatggctgg cttattgtga ttctgcacgc aacccagcaa
    16081 tgccagacat ggcagccctg ccagccacag ccgtcggggc aataatcgtt gaaaaatgtg
    16141 tcgcatattc accagactta aagcctatcc cagtgggcgt aattgttgca gacagtctgg
    16201 acatggacag cgcggagaaa ccggagcgta catatcgtac gtgaagattt cgagcactgc
    16261 ccggggccga actgacaaat aaaatagcct gataggatag gctgttagca ttatttcgtg
    16321 tttttcgacg acggtaattc aatgcggaaa caaacgtctt gcccgctctc gtcgaccaga
    16381 tacagttccc cttgcatacg gcgaatacaa tccctggcaa tgcttaatcc cagaccgctg
    16441 cccttcaccg ccccttttcg ctggtggctt ccctgaaaaa agggttcgaa gatcatggcg
    16501 cgttcctctt gcggaatggg cgtgcctgta ttgatgacat caatataaac ccgcgcacca
    16561 tgtgaactgc tgcgaaggca aatgttaccg gattcagccc cgtagtgcac cgcattggag
    16621 taaagattat ccagtacgct catcagcagc attggctccg ccaggcaagc tgttgctttg
    16681 agatcgacgt cggtatgcat cattttagcc cgtgcgggca ggctatgagc agaaaccact
    16741 gtctccacca gcggtgctaa ttcaacattc tccagttcca ccgcactgtc cgcctgttta
    16801 cggttgtaat caagcagttg ttcgatcagt ttttgcaaat tgcggctgct gctatcaaga
    16861 atgctcacca cctctttttg ctctggcgta agcggcccga caacctggtc agccagtaat
    16921 tcagtgccct cgcgcatact cgccagtggc gtttttaatt catgagataa atgtcttaaa
    16981 aattgatggc gttgggattc cagccatgac aggcgctcac ttaaccaaag aatacgttgc
    17041 ccaaccgagc gtaactcgct cggtccactg aacgaaacgc tattgcccag agaacgccct
    17101 tcccccagac ggttgatcat gcgctcgata tttttcaccg gcccgataat catccgcgtg
    17161 aaaagtagca ccatcaccag actcaccaga aatagcacca gcgattgcca accaaaatat
    17221 tgcccacgtt cggcgatttc acgctgaagt tgctgcccac gagagaacac cactgtgcgc
    17281 gtggcctgca ccatttcggt attggcactg gcaaaggctt caagacgcgc ggcggcggca
    17341 gcatcgggac cactgttgtt acactgaagt tgggccagat tgttcaaatc ctgacgcaat
    17401 gcctggtaaa gtttatcgtc tggcagtacg cctgcgtggg catcgagcat ttcgctgtaa
    17461 cgcttgcgct ggctttgata aaccttcgca agcgtagggt cgtccagcac gcaatactga
    17521 cggtagctac gctccatctc cagcgccgcg ttggtcatcg cttcactacg ccgggcatca
    17581 ataagcgtag tgcggttaac cagcgccgcc tgatcgctaa gcgcattcag gctttgccag
    17641 gcttgccatg ccagcaccaa caggggtagt agaattagca aaaatgccag cattaccagt
    17701 tgtcgtaatg agcggggaaa aacgggccag cgtttcaagg tgttactctc gtcagacgcg
    17761 aatagcctga tgctaaccga ggggaagttc agatacaaca aagccgggaa ttacccggct
    17821 ttgttatgga ataaggcggt gcctaactcg acgtttcgcc cgatggttga tatagctacg
    17881 ctgatatcag aagttggacg gcaggcacct tgttgtgcgt cattcgaatt ttatgtagca
    17941 cgtcccgaag gggctgacat aagtcggtga atgagccact gcttactatt atgcagtaac
    18001 tgtgccaata aagaaaatag tttggtaacg tactgattat acgttgcttt gagggattta
    18061 tggcttcttc gctatttgaa tgtaaatcag ccactgtgtc gctaaaaaga gacaacttaa
    18121 tataaactta ttagattaca ataaaatcaa tcatttaagt gtcgccagaa agcgacacgg
    18181 taaaccaccc attgtcgtga tttacagaca caaaaaagct cccggagttg ggagcttatg
    18241 atagtggttg gtgcttaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    18301 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    18361 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    18421 tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag
    18481 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    18541 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    18601 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    18661 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg
    18721 gccacaactc gctccccggg atcagttcac gcaggttaga catcatctgg caaccgttac
    18781 atacccccag cgccagcgtt tgcggacggt ggaagaaggt tgcaaactca tcgcgtacac
    18841 ggtcattgaa caggattgac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    18901 agaaaccacc gcacgcgacc agggcgtgga aatcttccag gcccgtgcgt ccggtcagca
    18961 ggtcactcat atgcacgtcg atagcatcaa agcctgcacg gtggaaagct gccgccattt
    19021 caacatgcga gttcacgccc tgctcacgca gcacagcaac tttcggacgt gcgccagtgg
    19081 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagtttcaca ttcaggcccg
    19141 gatcggcgtc gtttgatttc gcctggtgct cctggtcagc acactccggg ttgtcacgca
    19201 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtggtg cggctttcgc
    19261 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccta
    19321 cataatggac acaatcagca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    19381 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    19441 cgtcgcccag agaggcaata tccgcgttaa tgccgcaatg accagcaaag gccatttccg
    19501 ccagcgttac cagcaggccg ccatcagagc ggtcgtggta tgcgagcagc ttacgctgtg
    19561 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg cgcgacgtcg cgtacatctg
    19621 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    19681 tgcctttgcc caaatcaatc agcagcagtg tgttatcttc ggtagaaagc tgtggcgtga
    19741 tggtgtgacg cacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    19801 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    19861 ccaccgggat cgtcaggccc agcgccggac aaagctcttc gcccacggct ttaacggctt
    19921 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    19981 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    20041 ccagacgggc agaggcggcg aaatccagca gtgcaaccgg cgcacgctcg ccaatcgcca
    20101 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    20161 cctgccacgg ccccaccatc tgatcgcgcg ctaccatgcc ggttacgctg cggtcgccga
    20221 tggtcaccag gaaggttttc tctgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    20281 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    20341 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    20401 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    20461 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    20521 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    20581 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgcccgccg tcgctcacca
    20641 gttccggcat cgcgttagaa agaccgccag cgccgacgtc gtggataaac aggattgggt
    20701 tggcatcacc aagctgccag caacggtcga tcacttcctg gcagcgacgc tccatctccg
    20761 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacct gacgccatgg
    20821 aagacgccgc tccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    20881 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    20941 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccgttgtgg ctgttcactt
    21001 tttcttcata agtacggaag tagccattca gtgccggacg accaaattcg ttgttaaacg
    21061 ccgcgccgcc cagcgggcct tcggtcatga tgtccagcgc ggtgacaatg cgctcaggct
    21121 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gaaacggaga
    21181 aaccaaccag accagccttc ggcttcgcac cgcgtccggt ggcgccttca tcacgaattt
    21241 cgccgccgga acccgtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    21301 tttcgacttt catcagaata tgcgccggtt cctgatggaa atcgtagcgg cccgtttcgt
    21361 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    21421 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcttg aacagcgatt
    21481 tcggctgctg ttcaccgtcg ataatccagt cggcattaaa aattttgtgg cggcagtgct
    21541 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    21601 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaggcca agacgcaggt
    21661 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgctggta accggagtcg
    21721 gttgatggtg ggcaaacaac tgctctgcat catctaaagc aaaaaagacc gtttccatca
    21781 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    21841 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    21901 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    21961 ggagtttgcc ttgcggggcg tggctggcga gtgccgggcc atatttcagc aggcgttcaa
    22021 gttgtgcgtg ctcatcatcg tttaacggcg cattgaggtc agcaaaatgg acatactcgg
    22081 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    22141 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    22201 agctttcggt gtacccaagg gggggaaacg ggcgtcatta taaagaatct gatgcgctga
    22261 cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt gccgccaatg
    22321 gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt tatgagtaaa
    22381 cctttcacta ttattacgtt ttttcaagct gggacgcgca cgacacagag aattaactaa
    22441 ttgaaaaaat taaagattaa ttatctgttc atcggcattc tggcactgct gctcgcggtc
    22501 gctctctggc catccattcc ctggtttggt aaagccgaca accgtatcgc cgccattcaa
    22561 gcgcggggag agttgcgtgt gagcaccatt catactcccc tgacttataa cgaaatcaac
    22621 gggaaacctt ttggcctgga ttacgaactg gcgaaacagt ttgccgatta cctcggtgta
    22681 aaactgaaag tgaccgtgcg gcagaatatc agccagctgt ttgacgacct cgataatggt
    22741 aacgccgacc tgctggcggc aggactggtc tataacagtg agcgggtaaa aaattatcag
    22801 cctggcccta cctattattc cgtgtcacaa caactggttt ataaagtgag tcagtatcgc
    22861 ccgcgcacac tgggcaacct gacggcggag caactcaccg ttgcaccggg tcacgtggtg
    22921 gttaacgatc tccaaaccct gaaagacaca aaattcccgg aattaagctg gaaggtagac
    22981 gacaaaaaag gctctgcgga attaatggaa gatgtcatcg aaggaaaact cgattacacc
    23041 attgctgatt ctgtcgccat cagcctgttt cagcgcgttc acccggagct cgccgtagcg
    23101 ctcaatatca ccgatgaaca accggtgact tggtttagcc cgttagatgg cgataatacc
    23161 ctttccgccg ccctgctcga cttcttcaac gaaatgaatg aagactgtac gctggcacgc
    23221 attgaagaga aatacctggg gcatggcgat gattttgatt acgtcgatac gcgcacattt
    23281 ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa atacgccgaa
    23341 gaaattgact ggcgtttgct ggccgctatt gcttatcagg aatcgcactg ggatgcacag
    23401 gccacttcac cgacgggtgt gcgcggcatg atgatgttaa ccaaaaatac cgcgcaaagc
    23461 ctcggcatta cggatcgtac cgatgccgaa cagagcatca gcggtggcgt gcgttatttg
    23521 caggatatga tgagtaaagt gccggaaagt gtgccggaga acgagcggat ctggtttgcc
    23581 ctcgctgcgt acaatatggg ctatgcgcat atgctggatg cccgcgccct gacggcaaaa
    23641 accaaaggga atcctgacag ttgggctgac gtaaaacagc gtctgccttt acttagccag
    23701 aaaccctatt acagcaagct gacttacggc tacgctcgtg gacatgaagc ctacgcttat
    23761 gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt atctgcaaga gaaagagaag
    23821 caggctacag aagcggcgat gcaactggcg caggattatc cggcggtatc gcctacggag
    23881 ttgggcaaag agaaatttcc ttttctctcg tttctttccc agtcgtcatc aaactatttg
    23941 acccattctc cctctctgct gttttccagg aaagggagtg aagagaaaca aaattaatcc
    24001 gtcgagggtt gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg aaagaagtca
    24061 ctgagcaacg ccgcgcactc atccgccagt attccttccg taatttccac tcggtgattc
    24121 atacccggat gatgcagcac atccattaaa gatcccgcag cgccagtttt cgcgtcacgc
    24181 gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg ctccggcaca cattacacat
    24241 ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat aattttgcat caccagacca
    24301 ccctgccgca gggccatgat ttctgcatgt gcggtgggat catggcgacc aatcgggcgg
    24361 ttccagcctt cgccgattac ccgattgtta tgcactaata ccgcgccgac cggcacttcc
    24421 cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac gcatccagta ttcgtggcta
    24481 aattcgactt cagacaaaaa gaaaactccg gttataaaag cgcggcgcat tatacacgga
    24541 cgctatgctt tactctattc cagttgctgg agttcaccgc gcggggttac tcgccaacga
    24601 tgctgacaga aataaagcag cggattgtcc tgattactgt cgctatagcc actgtacagc
    24661 cgcagcggag tgccgatttt gcgctccagt tgtgcgacct tttcatgtcc cagacaacgc
    24721 atcgtcaata cccaaccacc atagccacgc tgaatttggc tggcgataag attaacccgc
    24781 ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga gccggtaatc
    24841 aaccagatat cagcatcgga acttaacagg taggtggtta atcgctcctg aaccagcgga
    24901 aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg cctgcaacgt ctgtaaacgt
    24961 gcttcgctgt gaccaaaagt gcacccccac agaagcagac tcatcggcca gcgtgccgca
    25021 cgacctttta tcaataacgc aatggctata atcggtaaca acggcaggac aagtaacgca
    25081 ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc cgaacatatc ctgctgatgc
    25141 aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa accatactcc
    25201 tctggactga aacatcctga ttaattttcg ctactacata gcctaacaga tagatcatca
    25261 cttttccggc aacaatcgac cgcatcatgg ctaactggaa tttttaattc atggcaatta
    25321 gcggcaatgg aatataaaat tcactcgcgt gtgtctcata ttgcctgttg tcgccactat
    25381 ttcatcgcga ggaaagatat gaacggctta cttcgtatcc gtcagcgtta ccaggggctt
    25441 gcccagagtg ataaaaaact ggcggattat ctgctgctac aacctgatac ggcgcgccat
    25501 ttaagctctc agcaactggc caacgaagcc ggagtcagtc agtccagcgt cgtgaagttc
    25561 gcgcaaaaac tcggctataa aggttttccg gcgcttaagt tggcgttgag tgaagcgctg
    25621 gcaagccagc cggaatcgcc ctccgtgccc attcataacc aaatccgcgg tgatgatccg
    25681 ttacggctgg tcggcgaaaa actgattaaa gaaaatactg ccgctatgta cgcaacgcta
    25741 aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa tgttgcgctc tgcgcggcgg
    25801 ataattctga ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc ctggaagctg
    25861 atgaagattg gcttcaatgc tgccgcagtg cgcgatatgc atgcgctact cgcaacagta
    25921 caggcgtcgt cccctgacga tctgttatta gccatttcct acaccggtgt acgacgcgag
    25981 ttaaacctgg cggcagatga gatgctgcga gtgggcggaa aagtgctggc gattaccggc
    26041 tttactccga atgccctgca acagcgtgct tctcattgcc tgtataccat tgccgaagaa
    26101 caagcgacaa acagtgcttc aatctctgct tgtcacgctc agggaatgtt aacggatttg
    26161 ctgttcattg cgctgattca gcaggatctg gaactggcac cagaacgtat tcgtcatagt
    26221 gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc gtacaatgcc cgcccggttt
    26281 gtgttgtttt gagagtttcc ttatggcgtt gttaatcact aaaaaatgca tcaattgtga
    26341 tatgtgtgaa atcaaataaa aacacattta ttttcatagt gatacagcaa tgtgttattg
    26401 aatgtgttgt tggatttatg gtgcaaaaaa attttgcata agttcagcaa cacaaagaac
    26461 accactttct ttaacgtcct ccgcgcccta aaggacgggg ctttacggcg caaaaggcct
    26521 gtgacatacc acattaactt ttcagtcaac cgtagcacgt tcttgtatac gccgtgtctg
    26581 cggttttata taaccctggc tggaaacctc ttatacaaaa aagtcgattc gccaacatca
    26641 tagatgatcg ccaccttctg gcgagaaact cctgatgcaa ttagtcgccc ggcctgcgcc
    26701 cattattctg gtgaaagttt gggacggcga ccgccaattc gtccctgtgc gcgagcagct
    26761 tccagtcctg cttttgttcg ttcaacaatc agttcacgtt ccatttcagc cagggcaccc
    26821 atcacatgga agaaaaagcg tcccattggt gtgctggtat caattgaatc agtcagacta
    26881 cgaaagttga tgcctcgttc gcgcaactcc tccaccagca cgacaagatg ccgcatactg
    26941 cgccccagcc gatccagctt ccagacaacc agtgtgtcgc ctgccgataa tgtcctgagc
    27001 agttttttca gccccggcct ttcggacttt gtgccactta tcttgtcttc aaaaatcagc
    27061 ttgcatcctg cacagttcaa cgcattacgt tgtagatcgg tattctggtc atttgttgat
    27121 acgcgtacat agccaataag catggt
//
LOCUS       DAZRUF010000028        27105 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZRUF010000028 DAZRUF010000000
VERSION     DAZRUF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.028"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..921
                     /locus_tag="ACHXOL_000969"
     CDS             <1..921
                     /locus_tag="ACHXOL_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6808017.1"
                     /translation="SATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLN
                     DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVN
                     IGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLT
                     APQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQM
                     RIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTV
                     NMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="ACHXOL_000970"
     CDS             complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="ACHXOL_000970"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR6808018.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1888..3924
                     /gene="lpoA"
                     /locus_tag="ACHXOL_000971"
     CDS             1888..3924
                     /gene="lpoA"
                     /locus_tag="ACHXOL_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR6808019.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3882..4277
                     /locus_tag="ACHXOL_000972"
     CDS             3882..4277
                     /locus_tag="ACHXOL_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR6808020.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4297..4887
                     /gene="diaA"
                     /locus_tag="ACHXOL_000973"
     CDS             4297..4887
                     /gene="diaA"
                     /locus_tag="ACHXOL_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR6808021.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4897..5472
                     /gene="dolP"
                     /locus_tag="ACHXOL_000974"
     CDS             4897..5472
                     /gene="dolP"
                     /locus_tag="ACHXOL_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR6808022.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5586..6626)
                     /locus_tag="ACHXOL_000975"
     CDS             complement(5586..6626)
                     /locus_tag="ACHXOL_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR6808023.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6699..7334)
                     /locus_tag="ACHXOL_000976"
     CDS             complement(6699..7334)
                     /locus_tag="ACHXOL_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR6808024.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7462..7980
                     /gene="yhbO"
                     /locus_tag="ACHXOL_000977"
     CDS             7462..7980
                     /gene="yhbO"
                     /locus_tag="ACHXOL_000977"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR6808025.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7960..8403)
                     /locus_tag="ACHXOL_000978"
     CDS             complement(7960..8403)
                     /locus_tag="ACHXOL_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR6808026.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8454..8756
                     /gene="yhbQ"
                     /locus_tag="ACHXOL_000979"
     CDS             8454..8756
                     /gene="yhbQ"
                     /locus_tag="ACHXOL_000979"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR6808027.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8743..9246)
                     /locus_tag="ACHXOL_000980"
     CDS             complement(8743..9246)
                     /locus_tag="ACHXOL_000980"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR6808028.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9240..9764)
                     /locus_tag="ACHXOL_000981"
     CDS             complement(9240..9764)
                     /locus_tag="ACHXOL_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR6808029.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9973..10968
                     /locus_tag="ACHXOL_000982"
     CDS             9973..10968
                     /locus_tag="ACHXOL_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR6808030.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10977..11855
                     /locus_tag="ACHXOL_000983"
     CDS             10977..11855
                     /locus_tag="ACHXOL_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR6808031.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDKLGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12061..13068
                     /locus_tag="ACHXOL_000984"
     CDS             12061..13068
                     /locus_tag="ACHXOL_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR6808032.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="ACHXOL_000985"
     CDS             complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="ACHXOL_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR6808033.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="ACHXOL_000986"
     CDS             complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="ACHXOL_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR6808034.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="ACHXOL_000987"
     CDS             complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="ACHXOL_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR6808035.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="ACHXOL_000988"
     CDS             complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="ACHXOL_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR6808036.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="ACHXOL_000989"
     CDS             complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="ACHXOL_000989"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR6808037.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="ACHXOL_000990"
     CDS             complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="ACHXOL_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR6808038.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20446..21390)
                     /gene="truB"
                     /locus_tag="ACHXOL_000991"
     CDS             complement(20446..21390)
                     /gene="truB"
                     /locus_tag="ACHXOL_000991"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR6808039.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="ACHXOL_000992"
     CDS             complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="ACHXOL_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR6808040.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21955..24627)
                     /gene="infB"
                     /locus_tag="ACHXOL_000993"
     CDS             complement(21955..24627)
                     /gene="infB"
                     /locus_tag="ACHXOL_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR6808041.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="ACHXOL_000994"
     CDS             complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="ACHXOL_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR6808042.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="ACHXOL_000995"
     CDS             complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="ACHXOL_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR6808043.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26826..26902)
                     /locus_tag="ACHXOL_000996"
     tRNA            complement(26826..26902)
                     /locus_tag="ACHXOL_000996"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26866..26868),aa:Met,seq:cat)
BASE COUNT         6423 a         7515 c         6809 g         6358 t
ORIGIN      
        1 tctgcaacgc taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca
       61 gttaaacttg tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat
      121 tacaaactca atgacagcct ggatattaaa accacattac aggcaaacga cattccagga
      181 ctcacaaccg accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc
      241 gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc
      301 gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg
      361 atgaccattc cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga
      421 agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc
      481 gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc
      541 aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt
      601 gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc
      661 gacggtacaa ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac
      721 aatgtgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct
      781 tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc
      841 tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg caccgccacc
      901 attaccgtca tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca
      961 ctgttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat
     1021 ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt
     1081 gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac
     1141 catttcgcct ttgcgacggt tttcatcttc ctttacccac gccagcagct cgccaacggg
     1201 cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga
     1261 ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg tagattcata
     1321 aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga
     1381 tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa
     1441 cgcagtgata gcagcacacg gtccgggtag cggcaccaca cgaatccccg cttcacggca
     1501 ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag
     1561 cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt
     1621 atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt
     1681 gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg
     1741 ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt
     1801 atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt
     1861 gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     1921 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     1981 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     2041 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     2101 gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa
     2161 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     2221 cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa
     2281 caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     2341 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag
     2401 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     2461 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     2521 tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt
     2581 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     2641 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     2701 tttggtcgca ccattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     2761 gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc
     2821 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct
     2881 gcggcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc
     2941 gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc
     3001 ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac
     3061 gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa ccagccagag
     3121 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga cgaagcgcgc
     3181 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     3241 agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc
     3301 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     3361 ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc
     3421 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     3481 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     3541 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa tgctgtacgc cagctcccgc
     3601 agcgcacaag gcaccgcagg cccggatttc cgtctggaga tggaaggttt gcagtacagc
     3661 gaaatcccga tgctggcggg cggtaatctg ccgttaatgc agcaggcact cagcgcggtg
     3721 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     3781 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     3841 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     3901 ggtcaggtag tccccgtcag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag
     3961 cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg
     4021 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     4081 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     4141 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     4201 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     4261 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     4321 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     4381 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     4441 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     4501 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     4561 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     4621 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca
     4681 gttgaagccg ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc
     4741 gagcttgcag gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt
     4801 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     4861 acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca
     4921 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     4981 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     5041 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc
     5101 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     5161 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     5221 aacgagattc gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc
     5281 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     5341 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     5401 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     5461 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     5521 tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     5581 tcaaatcaga ataacagcgc cagcccgccg acaatcacgc ctgacactgc caccatcgcc
     5641 cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa
     5701 ctcactgccg ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc
     5761 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     5821 accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat
     5881 aaccagacgc gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
     5941 cagaaaagtg tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt
     6001 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
     6061 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
     6121 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
     6181 aatgccagcg caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc
     6241 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
     6301 aagcgcgatt gtccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
     6361 gaaccgagga tcacccccag caccgccgct ttccataccg cgaggaaata gatcatcgcg
     6421 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
     6481 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
     6541 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
     6601 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
     6661 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
     6721 tctgaagagc ttaaaatagt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg
     6781 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggcagtaa acgaaacagc
     6841 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagcaa catcgacggg
     6901 cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg
     6961 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
     7021 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
     7081 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
     7141 atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta
     7201 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
     7261 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
     7321 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
     7381 caccaggctt aatagaccat gaggtaatta tccccgattg tgtgaaattc gcctcatcca
     7441 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcag tgatgaattt
     7501 gaggattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
     7561 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
     7621 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggt
     7681 ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
     7741 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
     7801 gatgtgattc gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat
     7861 gcgggcgcgg aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc
     7921 cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga
     7981 gtcgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcagtga atttgatttc
     8041 atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt
     8101 gtacgcctgg cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa
     8161 ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc
     8221 agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc
     8281 gactttctgc gcatcaaaca gataaaaagc attagcgcac cacagttccc cttcctgctg
     8341 cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc
     8401 cattgctgct ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac
     8461 cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg
     8521 atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga
     8581 aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg
     8641 cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg
     8701 cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg
     8761 ctcgtgatac tcaaccagac cggttacgcc attcagcgcg tcatccgcta aacgatgtac
     8821 ctggaaggcg ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc
     8881 aaagccgaaa cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa
     8941 ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta
     9001 tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc
     9061 aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc
     9121 cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc
     9181 gaatgagcga cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat
     9241 tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca
     9301 acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca
     9361 atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc
     9421 cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg
     9481 ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca
     9541 ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg
     9601 ccttcaagaa actccagctc accatcatcc agcgcctgac ggaattgcca gcttaagacc
     9661 tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga
     9721 gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt
     9781 ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat
     9841 tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc
     9901 gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgaa agcgcaacct
     9961 tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc
    10021 atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgatactaa tgcccgtcac
    10081 ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa
    10141 catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt
    10201 tggcagcgcg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac
    10261 ctcgccatgc tggagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg
    10321 caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt tgatgttgcc
    10381 cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacagctggc acgggtcaca
    10441 cctgtcccgc tggaagtctt tgcttttggc agcctgtgca ttatgtcgga aggtcgttgc
    10501 tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg tgggcgcgtg ttctccggcc
    10561 cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg
    10621 atcgaccgtt atcaggatgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat
    10681 ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa
    10741 ctgctgccgg agttaatggc ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt
    10801 agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag
    10861 gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc
    10921 gaaggcacgc agaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga
    10981 aatattcctt agggccagtg ctgtggtact ggccaaaaga ggcgctggaa gaattttatc
    11041 agcaggccgc caccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc
    11101 gccgggcaac caaagttggc gactggctgg agatggcaaa atcgctcgcc ggaagcggga
    11161 agcagatagt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga
    11221 aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata
    11281 tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg
    11341 tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt
    11401 cccgcgactg gctggtgaat ctgcttaatc agtgcgataa gttggggatt cgcaaccagt
    11461 ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta
    11521 ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga
    11581 acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga
    11641 ccatgagcgg ctacgtttac aacctcggta acgagctgac atccatgcag ggattggttg
    11701 atgtggtccg cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg
    11761 ctaatgaaaa tggcgctgcg ccactgccgt tgacggcaaa cagcgactgt aacggctact
    11821 ggcggcggct ggctgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag
    11881 ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg
    11941 attcactctc ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta
    12001 acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt
    12061 atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct
    12121 tcagcgcgag aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc
    12181 tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg
    12241 tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc
    12301 gtgatgttgc ctaaccactc accgttggtc attgccgaac agttcggcac gctcaataca
    12361 ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc caggtagtga ccaacggaca
    12421 atgatggcgc tgcgtcgcca tatgagcggc gatattgata atttcccccg cgatgtcgcg
    12481 gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc
    12541 tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg
    12601 gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg cgccggatat gttgttccag
    12661 gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg
    12721 atggtgtgca tcaatattat cgccgccgac agcaaccgcg acgctgaatt tctatttacc
    12781 tcaatgcagc aagcctttgt gaagctgcgc cgtggcgaaa ccggacaact gccgccgccg
    12841 attcaaaata tggatcagtt ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg
    12901 agtatgtcgt tggtgggtga taaagcgaaa gtgcgtcacg gcttgcagtc gatcctgcgc
    12961 gaaaccgacg ccgatgaaat tatggttaac gggcagattt tcgaccacca ggcgcgcctg
    13021 cattcgtttg aactggcgat ggatgtgaag gaagagttgt tgggatagtg tgtcttaacg
    13081 cgggaagcct tatccgagct ggcaacgctg tcctacgtag acctgataag cgaagcgcat
    13141 caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg
    13201 cagcaaatta aagctcgata agatatggac cagtgcgttg ccaacgccaa acaccagaat
    13261 cagcgtaatc atcggcttgc cgccccagac acggaatttc gggctgccaa agcgtttacg
    13321 cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag atggtagctg ctaaaccagc
    13381 ataaccaatg gcgtacagga atccgttcgg gaacagtaga ccacccacaa caggcggggc
    13441 aaaggtcagc aatgccgttt tcaagcgccc cagggctgag tcatcgaaac caaacagatc
    13501 tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt
    13561 tgagaacacg accagcagaa ggtccaggct acggctgttc agcacgccgc ttaatgcctg
    13621 taccagcaca tcaatattac cgcccttctc tgcaataccg ataaactccg gacgcgggat
    13681 gttacccatc gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta
    13741 caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggctcggcac
    13801 gttaccgtga taaccaaacg atgccagaca gaacggcagg gtcatcagca aatacggcgc
    13861 ataagatgca ttgctttcgg cgacgttgaa caatgtcgca ggctgcacat gccccagtag
    13921 gctaccgaag gtgaggaaga aggtaatgac tttcgccccc agtacaatcg ccgtcatgcg
    13981 gctgacggcc ttagtactca accacaccac aaacgctacc agcagtgcaa aaccaaaacc
    14041 cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca gaattgaacc
    14101 actggcagaa atataggcat aggtcaggat atagagcaca aaggcaatgg aaataccgtt
    14161 gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat
    14221 tcggtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt
    14281 aaagatcagc gccgccattg accagaaaaa ccacgccccg gacatgacca ctggcagaga
    14341 aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg
    14401 tgacgtttgg gtggtggtta gtgttgccat gagagcttct ctccagtgaa aaatagtgcg
    14461 actgcgttgt tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc
    14521 ccgatggtaa aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga
    14581 tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg
    14641 gccttcacga cgttcgccgc taaaacgacg accatcacca cggccacctt cacggcgttc
    14701 accgctgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg
    14761 acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt
    14821 gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc
    14881 gatggtggag tgagaagcaa acagcttgat gttaccaatg taacggctac tgatgtcgcc
    14941 ttcgttagca atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc
    15001 aatgcggtac agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg
    15061 gtcaccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa
    15121 ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt caccctgtgc
    15181 cattttcagc agtgccgcag ccagagtttc gagatccagc tcttcacctt ctgcagtcgg
    15241 ctgaattttg ctcagcagtg cgcggtattg atccagatcg ctgctttcca gctgctgctg
    15301 tactttagcg gcgaattttt ccagacggcg tttgcccagc agttctgcgt tcggcagttc
    15361 tacttccgga atagtcagct tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg
    15421 gttctcaacg aacagcagcg cgcggccagc acgacccgca cgaccggtac gaccgatacg
    15481 gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc
    15541 aacgtccagg ccacgggctg caacgtcggt cgcaatcagg atgtccagac gaccatcttt
    15601 caggcgttcc agtgtctgtt cacgcagcgc ctggttcatg tcaccgttca gcgcggcgct
    15661 gttgtagccg ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt tggtacgaac
    15721 gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt
    15781 gcgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga
    15841 ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc
    15901 cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt
    15961 ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc
    16021 gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt
    16081 accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc
    16141 gccgtacaga gcaaccacat ttacgccgcg catgtgttta gagaaatccg tcattgcttc
    16201 agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag
    16261 ctcaggatca agattctgca acagaggtaa agagaatgct gcagtttttc cgctccccgt
    16321 ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat
    16381 tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc
    16441 cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg
    16501 cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac
    16561 cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat
    16621 caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca
    16681 tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta
    16741 tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga
    16801 cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg
    16861 ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccagg
    16921 tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc
    16981 aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta
    17041 cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg
    17101 tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga
    17161 tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt
    17221 aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc
    17281 gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca
    17341 tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata
    17401 cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt
    17461 ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag
    17521 cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc
    17581 ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg
    17641 caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc
    17701 tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc tagaactttc
    17761 accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg
    17821 atttgagaga tgtggaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag
    17881 tcaacgatac gggtcacttt accagtgtag acgcggccca cttcgatttc tgcagtgatc
    17941 tcttcgatac gacgaatagc atgtttcgct ttctcgccgt cggtcgctgc gatcttcaca
    18001 gtaccgtcat cttcgatttc gatggtagtg ccagtttctt cggtcagggc acggataaca
    18061 gagccgcctt taccgataac gtctttgatc ttatccgggt tgatcttgat ggtatggata
    18121 cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc cattacgccc
    18181 aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg
    18241 ataccttcaa ttttgatatc catctgcagt gcagagatac cgtcgcggga acctgcaact
    18301 ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag
    18361 ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt gattggcaca
    18421 cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga agaaccgttg
    18481 gattcggtga tttcagacac tacacgtacg gtgtacggga atttgtccat atccggcatg
    18541 actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa
    18601 ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggta
    18661 tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgagcggt acccagcgtt
    18721 gcagtaacca gtgcctgcgt ttcaccgcgg gtgaacagcg cagaaccgtg agtacgcggc
    18781 agcacgccag tacgcacatc cagaccacgg atcatatctt tttcacgacc gtcgatacgc
    18841 ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca
    18901 cccagttcgt tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat
    18961 ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcactc
    19021 agacgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc
    19081 tgccagtccc aacgcggttt accggcttct ttcactagtt cattgatgtt ctgaataaca
    19141 acctgctgtt gttcatgacc gaacactact gcgcccagca tctgatcttc gctcagcagt
    19201 tcagcttcag attcaaccat cagtacagcg gcttcagtac cggcaacaac cagatccagt
    19261 ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct
    19321 acgcgggcag caccaatcgg gccattgaac ggaataccag acagagacag tgctgcggaa
    19381 gcaccaatca tcgcgacgat atccgggtta acctgcgggt taacagaaac cacggtggcg
    19441 ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc
    19501 agacgcgcga tcagggtttc gccttcgctt ggacgacctt cacgacggaa gaagctaccc
    19561 gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagtgg gaagaagtcc
    19621 tgacctggtt tggctttttt ctgtccaaca acggtaacga ataccgcggt gtcatccatg
    19681 ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg
    19741 tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt
    19801 cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact
    19861 aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct
    19921 gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc
    19981 ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga
    20041 cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc
    20101 agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg
    20161 tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact
    20221 tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct
    20281 tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct
    20341 aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg
    20401 gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc
    20461 aaccaccagg cgacgaggcg cgacgcggcc ttcatcgtca atttcgccca taccgataaa
    20521 cttgccgttc tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg
    20581 aactgggtta ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta
    20641 gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat
    20701 atcctgctgt tcggcttgtt cgaccagttc acgcagatgt tccagggtca ccatccgttc
    20761 aaccggatat ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag
    20821 tttttcaccc aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc
    20881 cagctccagc tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg
    20941 cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg tacagttttt taccctgata
    21001 tttcagtgct gaatacatcg aagggatctg ttcgatatcg ccacggaaag tatccagcgc
    21061 cgccgccagt tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccgtcggc
    21121 atcagaagta tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc
    21181 cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgcctgttgc
    21241 cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt
    21301 ttgcagcgca tcattgctgg acataccctg cggtttatcc agcaacaaaa cgccgttaat
    21361 gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca
    21421 cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca
    21481 accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc
    21541 cccagcaggc tgcggatgaa accagaagct tcttgcaacg ctttgatgcc cgctttaacc
    21601 gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt tggcatacgc caggtcgcga
    21661 gacatttcga caccggaaac ggtggtcatc atgcccaggc gaggatcttt aatttcacgc
    21721 tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat
    21781 tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta
    21841 atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgccagcgt cacatcaggc
    21901 aatccatgcc ggatgcggcg taaacgcctt atcccgcatg gaaccctaaa aaccttaagc
    21961 gatggtacgt tggatctcaa tgatttcgaa tacttcgatc acatcgccag tgcggacgtc
    22021 gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc
    22081 tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt cacgcagaac
    22141 gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacagcctg cgatggcacc
    22201 aaatttcggc gatttgaaca cgtcacgaac ttccgccaga ccgataatct gctgtttcag
    22261 ttccggagac agcataccgc tcatcgccgc tttcacttcg tcaatcaggt tatagatgac
    22321 ggagtagtaa cgcagatcca agctttccgc ttcaatcact ttacgtgcag aggcatcagc
    22381 acgtacgttg aagccaacca ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc
    22441 ggtgatacca cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt
    22501 cagcaaggag tcggagatcg cttccacaga accctgtacg tctgccttca ggacgatgtt
    22561 cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg
    22621 ctgacgcgcc agtttaactt cgcggaattt accctgacga tacagtgcaa cttcacgcgc
    22681 tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggtacgc cggacaggcc
    22741 gaggatttcc accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg
    22801 catcgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt
    22861 accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat
    22921 aaccgcaccg ctcgccatac ctttacgtac cgctttcagc tccagaactt ccgcctgcag
    22981 caggatagcg tccagcagtt catcgatgcc ggtacccgct ttcgcagata cgtgtacgaa
    23041 ctggctttca ccgccccact cttccggcag gatgccatac tgggagagtt cgttcttaac
    23101 acgatccgga tcggcttctg gtttatcaat cttgttcact gcaaccacca ccggtacctg
    23161 cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc
    23221 aacaaccagg actacgatgt ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc
    23281 ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc
    23341 accaatgtgc tgggtaatgc cgcccgcttc gccagaggca actttcgttg aacgaatgta
    23401 gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg
    23461 cggttcagcc gcagcacccg tgtcacggtc gctcattacc gcctcttcca gctcgttttc
    23521 acgacgcagg ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc
    23581 gataacctgg ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga
    23641 gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac
    23701 gtcacggtta acggcctgag ctggcttctg gaagccttgc tgcagggaag aacctttacg
    23761 ttttccgcct ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc
    23821 agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac ggccacgacc
    23881 gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagtagtgac
    23941 gtgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc
    24001 catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac gatgcgcttc
    24061 ttcttcggct ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg
    24121 ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg
    24181 tttcgcttgt tcagcagctt cacgcttagc ttgttctgca gcctcacgtt cagctttttg
    24241 ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg
    24301 ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt
    24361 gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt
    24421 aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt
    24481 caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg
    24541 gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc
    24601 cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc
    24661 gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggcct tttcgtcggt
    24721 caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca
    24781 aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag
    24841 atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag
    24901 tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc
    24961 caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaagc cttcttctac
    25021 cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc
    25081 gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca
    25141 accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag
    25201 gttaccggct tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc
    25261 aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc gggttatcat cccacaggac
    25321 gatatcgata cgctcgccac ccagttcagt agacaccgcc tgaacacgcg cgccacgcat
    25381 acctacgcaa gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc
    25441 acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttctgg
    25501 cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg
    25561 cgcgccacgc gcttccgggc gaacggaata aagcacgcca cgaacgcggt cgccagggcg
    25621 gaagttttca cgcggcagca tatcttcacg caggatcacg gcttcagcgt tgttgcccag
    25681 atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc
    25741 gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat
    25801 aacctgtttt gccgtctggg tagtgatacg gtcaaaggta acagactcaa tctgatcttc
    25861 aacgtaatcg cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc
    25921 cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc
    25981 gcttttgcga tcgatctgta cgcggacgtc gatctcttgt tcatattttt tctttgttgc
    26041 tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt
    26101 ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc
    26161 agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc
    26221 atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg
    26281 ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa
    26341 acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag
    26401 gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac
    26461 atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga
    26521 tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc
    26581 agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa
    26641 aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg
    26701 ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt
    26761 tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg
    26821 gaaagtggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
    26881 taccaggctg ctccaccccg cgcctgaaac gtggtaaatt ctactcgttt tgggtaaaaa
    26941 atgcaaatac tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata
    27001 gtgatccacg ccacattttg tcaacgttta ttgctaatca tgtgaatgaa tatccagttc
    27061 actttcattt gttgaatact tttgccttct cctgctcccc cttaa
//
LOCUS       DAZRUF010000029        26858 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZRUF010000029 DAZRUF010000000
VERSION     DAZRUF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.029"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(84..806)
                     /gene="baeR"
                     /locus_tag="ACHXOL_000997"
     CDS             complement(84..806)
                     /gene="baeR"
                     /locus_tag="ACHXOL_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR6808044.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(803..2206)
                     /gene="baeS"
                     /locus_tag="ACHXOL_000998"
     CDS             complement(803..2206)
                     /gene="baeS"
                     /locus_tag="ACHXOL_000998"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR6808045.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2203..3618)
                     /locus_tag="ACHXOL_000999"
     CDS             complement(2203..3618)
                     /locus_tag="ACHXOL_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6808046.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3619..6696)
                     /gene="mdtC"
                     /locus_tag="ACHXOL_001000"
     CDS             complement(3619..6696)
                     /gene="mdtC"
                     /locus_tag="ACHXOL_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR6808047.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6697..9819)
                     /gene="mdtB"
                     /locus_tag="ACHXOL_001001"
     CDS             complement(6697..9819)
                     /gene="mdtB"
                     /locus_tag="ACHXOL_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR6808048.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(9819..11060)
                     /gene="mdtA"
                     /locus_tag="ACHXOL_001002"
     CDS             complement(9819..11060)
                     /gene="mdtA"
                     /locus_tag="ACHXOL_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR6808049.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            11339..11395
                     /locus_tag="ACHXOL_001003"
     CDS             11339..11395
                     /locus_tag="ACHXOL_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR6808050.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            11666..11722
                     /locus_tag="ACHXOL_001004"
     CDS             11666..11722
                     /locus_tag="ACHXOL_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR6808051.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            11995..12054
                     /gene="ibsA"
                     /locus_tag="ACHXOL_001005"
     CDS             11995..12054
                     /gene="ibsA"
                     /locus_tag="ACHXOL_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR6808052.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            12275..12934
                     /locus_tag="ACHXOL_001006"
     CDS             12275..12934
                     /locus_tag="ACHXOL_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR6808053.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            12931..13692
                     /gene="pphC"
                     /locus_tag="ACHXOL_001007"
     CDS             12931..13692
                     /gene="pphC"
                     /locus_tag="ACHXOL_001007"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR6808054.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            13689..15629
                     /gene="yegI"
                     /locus_tag="ACHXOL_001008"
     CDS             13689..15629
                     /gene="yegI"
                     /locus_tag="ACHXOL_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR6808055.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(15642..16994)
                     /gene="yegD"
                     /locus_tag="ACHXOL_001009"
     CDS             complement(15642..16994)
                     /gene="yegD"
                     /locus_tag="ACHXOL_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR6808056.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            17128..17976
                     /gene="alkA"
                     /locus_tag="ACHXOL_001010"
     CDS             17128..17976
                     /gene="alkA"
                     /locus_tag="ACHXOL_001010"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR6808057.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(18086..21403)
                     /locus_tag="ACHXOL_001011"
     CDS             complement(18086..21403)
                     /locus_tag="ACHXOL_001011"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR6808058.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            21721..22362
                     /gene="udk"
                     /locus_tag="ACHXOL_001012"
     CDS             21721..22362
                     /gene="udk"
                     /locus_tag="ACHXOL_001012"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR6808059.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            22454..23035
                     /gene="dcd"
                     /locus_tag="ACHXOL_001013"
     CDS             22454..23035
                     /gene="dcd"
                     /locus_tag="ACHXOL_001013"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR6808060.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            23057..24910
                     /gene="asmA"
                     /locus_tag="ACHXOL_001014"
     CDS             23057..24910
                     /gene="asmA"
                     /locus_tag="ACHXOL_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR6808061.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(25184..26767)
                     /locus_tag="ACHXOL_001015"
     CDS             complement(25184..26767)
                     /locus_tag="ACHXOL_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR6808062.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
BASE COUNT         6369 a         7186 c         7226 g         6077 t
ORIGIN      
        1 tcagtttgct gaccatgcgt gggataatgt tgccggatct ggtctgaacg ccatctccgg
       61 ctaacaaagt catgtcgcta aaactaaacg atgcggcagg cgtcggcttc ccagcggtaa
      121 ccaacgccgt aaacggcgcg gataaatgac tgttcggcgt cgagagattc cagcttgcgg
      181 cgcaggtttt taatgtggct gtcaatggtg cggtcggtta ctacgcggta gtcgtcataa
      241 agatgattga gcaattgctc gcgggagaac acttttcctg gttcatgaga gagcgttttc
      301 aacagacgaa attccgcagg cgtcaggtca agcattttac cgcgccatga agcctgaaaa
      361 cgaccttcgt cgataatcaa cgggctttcg gcatcctgtt gctgcaattc gcgctgcggt
      421 ttgcaacggc gcaaaatggt tttgacgcgc gctaccactt cccgtgggct gtacggcttg
      481 cagatgtagt catctgcgcc aatctccagt ccgagcaggc ggtcgatctc ttcgattttt
      541 gccgtcacca tcacgatagg aatgtcagaa aaacgacgaa tttcccggca cagcgtcaag
      601 ccatcggtgc cagggagcat cagatccaac aggatcagat ccggtggtgt ctggcgcaca
      661 tacggcagta cctgatcgcc gtggctgata agcgtcggcg catagctcgc agcacgcaga
      721 taatcaatga gcaactgccc cagcttcggt tcatcttcca cgatcaaaat acgcggtgtg
      781 ttttcgtcga ttggtaactc ggtcatactt ctctctgtaa atcccgttcc agcggtaact
      841 ctactgtaat gcttaccccg ccaaaaggcg aatgggcagc aataatgcgg ccattatgtg
      901 cttcaacaat gttcaggcaa atcgccagcc ccagcccgga accaccgctg gcgcggttgc
      961 gggaaccttc ggtgcgataa aaacgttcaa ataatttttg tagctgctcg tcactgacac
     1021 ctggcgcact gtcggcaaag gtcaggcgca ccgttttgtc gtgctgctcg gcagagattt
     1081 tcaggctgcc gccgctgtca gtgtagcgca ggctgttttc aagtaaatta ttgaataact
     1141 gcattaaacg gtcgcgatcg ccaaatacgg taatactgtc tggcagggaa aattgcagtt
     1201 tcaggccacg gctggcgaat cgttcgcgaa atgcgccgcc cgccacttcc agcagtggga
     1261 tcaaatctac cggtgctttt tgataggcga gggcgccttc atcagacatc gacaactgat
     1321 ggagatcgtc aaccagtttg gtcagtgtac cgacctccgc ctgtaaagac gccaccgtct
     1381 ccggcgtgaa tttgcgcacg ccatcctgaa tagcttccag ttcaccgcgc agcaccgcta
     1441 atggcgtacg cagttcgtga gaaatatcgg ccataaaatc gcgccgcatt tgctggtttt
     1501 tctccagtgt gctggcaagc tggttgaagt cttgcgccag tttgcccagt tcatcttcac
     1561 tggtgggcgt tacgcgggta gtgaaatcgc ccgccgccag tttgtgcgtg ccatcgacaa
     1621 gtcgttttac cggtgccagt aaaccgcgcg ccagcagaaa agtggcaagt gccgcgagta
     1681 acgttgccag ggcgacaatc aaccagctgg tttgccgctg ttgtttatcg aaattgatat
     1741 cagtattgcg cgttaaccgc tcaacggggg aggcgatcac cgcgccaact tccgcaccgt
     1801 tgaccagaat gggtcgccgt gtaccgtcag gtggaatcgg cgctcgcgga ccaaccagca
     1861 ctttgttgtt ttgatcaacc acccagaact gggtacgcca gccgtgcggt ggcatacccg
     1921 ggccgggttt atcttccgaa ttatcgtgtt caaatgaacg caggatctga aagacaaagc
     1981 gatcattgtt gcgcaggaag cgccagttgc catgctgcgc atactgctcg ccaagcgcat
     2041 cacttaacag ttgtaatcgc tgttcattac catgcttgat gtaatcaata aagccacgct
     2101 caaaactgat acgcaccgcc cagtgcatac tgatcagcaa aacaatgcag gtggcgaaaa
     2161 tcgccagaaa cagtttgccg gtaataccag gtcgccagaa cttcattgcg cgctcctttt
     2221 tcgccgcgaa atagctacat tttgatgcgt atcgttcggc actctggcaa agatgaacgc
     2281 cggaagggcg atgataaacg ccatgctaag ccaggtgtac ataaagacgg tttgtgtggt
     2341 gccgctgtcg acgctgacat gctgcgaacc aaaaagtccc agcaacagcc cggcgatggt
     2401 gacgccgata ctcatcgaca attgcataat catcgacagc agactgttgc cgctgctcgc
     2461 cagattgtcc gggagatctt tcagcgtcag ggtgttcatg gaggagaaac gggtcgagtt
     2521 gaccatccct tgtaaaaaca ggacgaacgg caaaacgtag taccagccca acagagcggt
     2581 agtcataaac aacagggtga tcagcgacag acccagcgtg gtcgctacca gtacccgacg
     2641 ataaccaaag cgattcacca cctgaaccac aattcgcttc attcccatgc tgccaagtac
     2701 catcgggatc atcatcagtc cggcatgaaa cggcgagaaa ccgaggccaa tttgcaggaa
     2761 aaccggtgtc ataaagggca acatgccact gccaatacgt ccggcaaagc tccccgccag
     2821 gcccagcgaa aaggtacgag tgcggaacag tttcagactg aacagggcgc ggttgttatt
     2881 tctggcgtgc agcagataaa gcaccagtgc caccacgcca actgcgacca ggccagcaat
     2941 cgccagcggc gataaacctg tacctttact gccgtccagc gccagggtta atacagccat
     3001 gccaaccgcc agcaataaaa agccggaaag atcaaagcgc cgcgtctgca tggtgtagtt
     3061 cggcattaac agcaatgtgg cgatcgcacc gataatcccc accggaatgt tgatcaaaaa
     3121 gatccagtgc cacgacgcgt actccaccag cagaccgccg agcgccggac cgagcagcgg
     3181 accgacctga ccgggtaacg tgacaaaggt catcgccgcc atatattgct cgcgcggtac
     3241 gattttcatc accgtcaacc tgccgaccgg caccatcatc gcaccgccaa cgccctgtaa
     3301 cgcgcgtgcc agcaacagtt cgttcagcgt gccggaaagc gcgcaaaaca gtgaaccgag
     3361 agtaaacagc acgatggcgg taaagaaaat attgcgcacg ccgactttgt ccgccagcca
     3421 gccgctggcg ggcagcatca ccgccacggt cagcacataa gagacaatga ccatgtgcat
     3481 atgcaacgga ctttccccga ggctttgcgc cattgaggga agggcggtgt taacgatggt
     3541 ggtgtccagc gactgcataa agaagccgaa agccacaatc cacaattgcc agcgggtgct
     3601 gtcgggaagt tctgtcattt actcggttac cgtttgttta ggtttacgcg aaaaacgcag
     3661 ccgcagacgg tcgaaaaaga gatacaccac cggcgtggta tacagcgtca gaagctggct
     3721 cattaccagt ccaccgacaa tggtgatccc cagggggtgc cgcagctccg agccgtcacc
     3781 gcccgacaac accagcggca gcgcgccaaa cagcgccgcc agggtagtca tcataatcgg
     3841 gcgaaaacgc agcagacagg cctggaaaat agcttcctgc ggcgtcaggt taccgtgccg
     3901 ttgggcttcg agcgcaaaat cgaccatcat aatggcgttt ttctttacga tgccgattaa
     3961 tagcatgatc cctatcaggg ctattaggct gaacggggca ttgaacagct ccagcgccag
     4021 cagcgcacca acgcccgccg agggcagggt ggagagaatc gtcagtgggt gtacgtaact
     4081 ctcgtaaagg atacccagca cgatatacac cgtggcgatg gcggcgataa tcaggatcac
     4141 ctgtgagttc atcgtctcct ggaacacctg cgctgttccg gcaaaactgc cgcgcaccgt
     4201 cgaaggcaca ccaagctggg tcattgcgcg atcgatcgcc gcactggcgt ccgagagcga
     4261 ttttccggtc ggcaggttga acgagatggt cgaggccgcc gataatccct gatgattcac
     4321 cgacagcgga gcgttcgccg gttgccattt agcgaaatac gacagcggga tcgctttgcc
     4381 ttcgttattg ataacgaaca ttttttccag cgcactgatg tcctgggtat agcgcggatc
     4441 cacttccatc accactttat actggttcat cggctggtaa atggtcgaga tttgccgctg
     4501 accgaaggcg ttatttaaca ggctgttggc ggcctgcacg tcgattccca gccgtgccat
     4561 ggtgtcgcgg tcgtaaacca gattcatctc cgcgccgtta tcctgctgat cggagttcac
     4621 gtccgccagt tccggcaacg tcgccagttt tttgcggatt ttcggctccc attcgcgcag
     4681 tgccgccagg tcgtcggata acaacgtgta ctggtagctg gcgttcgact gacgcccacc
     4741 aacgcgaata tcctgtaccg ccatcaggaa cagattcgcc cccggttctt tcgccagttt
     4801 tacgcgcaga cggtcgataa tttgctgcgc cgtttcgctg cgttcgtcgc gtggcttgag
     4861 ggtgataaac atcatcccgc tgttcactcg cgaaccgcct gtaaagccgg tgacattatc
     4921 cactgccgga tcgtcacgga taattttcat gaaatcctgc aacttaccgc gcatcgcctg
     4981 aaacgaaata ctctgatccg cctgaatccc gcccatcaac acgccagtgt cctgctccgg
     5041 gaagaaggtt ttcgggatcg agatatacag ccagatattc agcgcaatgg tgccaagcag
     5101 caccacgccc accagacggg tatgattgag cacccatttc agtgacttgc cgtagccttg
     5161 ttgcagggct accaacatgc gaccaaaacc acgcagtcgc ttttgctcgc gcggcttgct
     5221 ggctttcagc atccagccac acatcattgg cgttaatgtc agagaaacca gcaacgaaat
     5281 accaatggcg acagaaagcg tcacggcaaa ctcgcgtaac agtcggcccg gcaatccgcc
     5341 catcaacagc agcggcagga acaccgccac cagtgacaga ctcatcgaca gcaccgtaaa
     5401 accgacttcg cgagtacctt gcagtgcggc ttgcaacggt ttcattcccg cttccagatg
     5461 acgtgcaatg ttttccagca ccacgatggc gtcatccacc acgaaaccag tagcgatggt
     5521 gagcgccatt aacgaaaggt tattgagact gaatccgcac aggtacatcg ccgcaaacgt
     5581 accaatcagc gaaaccggca ccgaaacggc gggaataata gtggcgcgac ccgagcgcag
     5641 gaataaaaac accaccagaa tcaccagcgc caccgagata atcagcgttt gctcgacttc
     5701 ttccagcgag gcgcgaatgg tgggggagcg atcctgggca atttgcagat caatcgccgc
     5761 cggaatggtt tcctgcaact ccggtaattt tgcccggatg ctgtcaaccg tctggataat
     5821 attggcttcc ggcagtttgc ggatcatcag taaaatagcc ggtttggcgt tggtcatccc
     5881 ggcgttgcgc acatcctgca ctgagtcggt caccgtcgcc acatcgccca gacgaaccgc
     5941 gccgccgttg ttgtagtgaa taatcaacgg ctgatattca gcggcggttt ttagctcatc
     6001 attggtctgg atctgccagc ggtgagtgcc atcttccagc gcgccctgcg gtttacgcac
     6061 gttggcattg ctgatggcgg tgcgtacgtc gtccagcgac acgccctgat taaacagcgc
     6121 ctgcggattc agcccgacgc gtacggcggg cagtgagctg cctccgacat cgacatcacc
     6181 aacaccgtcg atttgcgaaa tcgtcggagc cagctgcgtc gaggcgaaat cgtacagttc
     6241 accctgcgaa taagtatcgg atgtcagcgt gaggatcata attggcgcat ccgacgggtt
     6301 cgctttgcga taggtcggac ggctgggcat cccactgggc agcaaacttt gtgcagcgtt
     6361 gatcgccgcc tgcacatcac gcgctgcgcc gttgatgtcc cggtcaaaat caaactgcaa
     6421 aataatacgt gtgctgccga gcgaactgct ggaggtcatt tcgctgaccc cggcaatgcg
     6481 cccaagcgag cgctccagcg gcgtggcaac ggaagacgcc attgtttctg gtgacgcacc
     6541 gggcagcgag gcgctgacca taatcaccgg aaaatcgacc tgcggcagcg gggcgaccgg
     6601 cagcatacgg aagcccagta tgccgcacag ggtaatggcg accgacagta aaatcgtcgc
     6661 caccgggcgg taaatgaaga gggcaaaaaa cttcacttac gcctcctctt catggcgagc
     6721 aaagcgactt ttggtccaca atgccaggcg gtcgaacagc agataaatca ccggcgtggt
     6781 gaacagcgtc agcacctggc tgacaatcag accgccgacc atgccgatac ctaacggacg
     6841 acgcagttcc gcgccgaccc cggtactcaa catcagcggc agcgcgccaa gcagagccgc
     6901 cagggtggtc atcaggatcg gacgaaaacg caacagacaa gcctggtaga ttgcatcgcg
     6961 cggcgacatg ccttgctcgc gctcagcagc cagcgcgaag tcgatcatca tgatggcgtt
     7021 cttcttcaca ataccgatca gcaaaataat gccgataatc gcaatcacat ccagttcgct
     7081 accggcaatc attaatgcca gcagcgcgcc gacccctgcg gtgggtagcg tcgagagaat
     7141 ggtgatcggg tgaataaagc tctcgtacag aatgccgagc acgatataca tcgccaccac
     7201 cgccgcgaca atcagccaga cagtgctgcc cagcgccgac tggaaggcga gggtgctgcc
     7261 ctggaattgc gtggtgatat ccaccggcag attcagcgtc ttttcggtgt ccataatctc
     7321 ctgcaccgca tcgcccagcg aatagttatc cggcacgtta aaggagatgg tcgttaccgg
     7381 gaactgatcc agatggttga tggaaagcgg cgcaaaacgc tgttcaactt tggcaattga
     7441 gcttaacggc accacgccgc cgtcgctact ggtcaggcga atggtatcca gcgccgcgag
     7501 gcccggggta atttcggtgt tgtgctccag cactacgcga tactggttag cctgagtata
     7561 aatagtggaa atcagtcgct gaccaaacgc gttgtacagg gcgttatcga catccgccat
     7621 gctgatcccc agacggctgg cgctgtcgcg atcaacattg acatacgcca ccagcccttg
     7681 gtcctgccag tcgctggaga catcagaaag ctgtggcagt tgctggagtt tttccatcaa
     7741 ctgtggcacc caggtactga gcgcatccag tgaggtggcc tgcaaggtaa actggtactg
     7801 ggtgcggctg acctgagtat caatagtcag atcctgcgtt ggttgcagga agagatcgac
     7861 gcccggcact ttatctaccg ccgtttgcag acgggcgatg actttttgca cccgatcatc
     7921 acgttcatcc aacggtttga ggttgatttg taaacgtgca ctattcagcg acgggttagt
     7981 gccatcaacg ccaacaaatg aggtcaggct ttgcactgcc ggatcctgca aaatcacgtc
     8041 cgcgacctgg cgttgtcgct gggccatatt ggtaaaggag ctggattgcg gtgcctgcaa
     8101 agtgccctga ataatgccat tgtcctgtac cgggaagaaa cctttcggaa tgaacaccca
     8161 cagcagcacg ctaagcagca gcgtgctgag tgccacgctt aaggtcagcc acggatgatt
     8221 cagcactttc gccagtccac gaccataggc ggcgattatc ctgtcgaaca ttttttccga
     8281 ggcacgggag aagcggttct gtttacgcaa cgactcctgg ctgagcatcc gcgcgcacat
     8341 catcggtgtc agggtcagcg acaccaccgc tgagatcaaa atcgctaccg ccagggtaat
     8401 agcaaattcg cggaacagtc gcccgacgat atcgcccata aacagcagtg ggatcaacac
     8461 cgcaatcagt gagaaggtca gcgagataat ggtaaagccg atttcacctg cgcccttgag
     8521 cgccgccgcc aacggttttt cgcctttttc gatatagcgg gaaatgtttt cgatcaccac
     8581 gatggcgtca tcgaccacga atccggtggc gatagttaac gccatcagtg tcaggttatt
     8641 gattgaaaaa tcgagaaaca ccataaccgc gaaagtgccg attaacgaca gcggtacagc
     8701 aacaccggga atgatggtcg ccggaatatt gcgcaaaaac aggtagataa tcatgactac
     8761 cagcgcgata gccatcatca attcaaactg agtatcatcg acggatgcgc ggatattggt
     8821 ggtgcgatcg gaaagcactg tcaccttcac cgatttcggc agactctcag tgagctgtgg
     8881 cagcatctgc cgaatgctgt cggcggtgga gataatgtta gcaccgggct ggcgctgaac
     8941 attcatcaca atggcctgtt ctttgttcgc ccacgcgccg agccagctgt tttctgcacc
     9001 ttgctctaca gttgcgacat cgcccagacg aattggcgcg ccgttctggt aggcgatgat
     9061 tagctggcga tactcttcgg cggattgcat ctggtcgttc gcggaaagcg tgactgcacg
     9121 ggaagggccg tcgaggctac cttttgccga gttaacgtta gcgccggtaa tggcggtgcg
     9181 cacggtttcg ctggtcaggc cgagggcggc aatcgcctga gcgttaagtt tgacgcgaac
     9241 agccggacgc tgaccgccgg aaagcgtcac caggccgacg ccggaaatct gcgagatttt
     9301 ctgcgcgacg cgggtttcca ccatatcttc cacttgcgtc atcggcatgg cggttgaggt
     9361 gacggcgagc gtcatgatcg gcggatctgc cgggttcact ttgctgtaaa ccggcgggtt
     9421 aggcagatcg ctcggcaaca agttggtcgc agcgttaatc gcggcctgca cttcctgctc
     9481 ggcgacatcg agcggtaatg ttagctggaa ctgcaaagtg ataactgacg caccgccgga
     9541 actttgcgac gacatctgtt tcaggccaga catctgcccg aactggcgtt cgagcggcgc
     9601 ggtaacggca gaggtcatga catccgggct ggcacctggg tagagcgtga ccacctgaat
     9661 ggtcggatag tccacttccg gcagcgccga aacgggcagg gcgcgataac cgataatccc
     9721 ggcgagtaag atcgccacca tcagcagcgt ggtggcgaca ggacgcataa taaacaggcg
     9781 cgacgggccg cctgtgctgc tcgggggtaa cacctgcatc aggagcgtgc tccttttttc
     9841 gcgtattcgc ggctggtggc tttctcttcc ggagtggtgg cgctctgggc ttccaccact
     9901 tccactttcg ccccttcggt caggcgatca atgccgtctg tcaccacgcg atcgcccgca
     9961 gaaatacctg cacggatcac cactttctga ctgtcctgaa tgcccggcgt caccagatgt
    10021 ttgctgacct tgttttcgct attcagcacc cagacaaaat ggccttcatt gcccatttgc
    10081 agggcggctg ttgggatcac tacggcgttt tgttcggtgt cgactaacat gcgcgcgtta
    10141 acaaactgat tgggaaacag cgcatcatcc tgattattaa agcgtgcttt cactttaatc
    10201 gtaccggttg tggcatcgat ttggttatcg aggcttaaca gcgtgccttc acttaatttc
    10261 ttcgaattgg tgcgatccca ggcttctacc accagcggtt ttccggcttt ttgcgcctgc
    10321 actacagtag cgatatcgct ttccggcagg gtaaagacta aatcgatagg atgcgtctgg
    10381 gtgatcacca caattccggt ggtatcacca ctggagattt ggttaccaac atcaacctgc
    10441 ttgagaccaa cgcgaccatc gactggtgcg gtgatgcggc tccagtcgag ttgcaactgc
    10501 gcgctggcga cgcttgcttc atcagcctta atggtgcctt cggtttcact gaccagcgcc
    10561 tgttgggcat ccagctcctg acgggagacg agattggttt ttgccagttg ctgataacgc
    10621 gccagatcac ggcgggcgtt agcaagcgtg gctttatctt ttgccagttg gccctgggtt
    10681 tgtgctaatg caactttgaa ctggctgggg tcaatttctg ccagtaaatc gcctgctttg
    10741 acctgctggc cttcctggaa atgtaacgcc atcagttgac cgtccacgcg gctgcgcacc
    10801 gtaacagtat tagcggcgat aatggtgcca agcccggtga ggtaacgcgg aactgcctgt
    10861 tctacggcgg tcgccgcctg aaccggggct aatgggccgg aacgcatacc acggcgacca
    10921 cccgctggcg attgctgcgc ttgtttcgtc gcccctgggg ctgcactctg ggagtcattg
    10981 cggccttgcc agaaccagaa tgcggcgatg gcggcgataa ccaccacgat tacccaacgg
    11041 gatttataac tgcctttcat cgttaagagt ttctcttcct gaaacgtttc gcggaatgat
    11101 actagtgtag ccagccaata agggagaaaa atggaggaat tatgaaacac tgtcgggaat
    11161 cgtctgaatg acgggcacat ttgcgagcac gcatccagta ataacacagg aaactatttt
    11221 atctacgcgt tagcgataga ctgcttgcat ggcgaaagga ggtaagccgc cgatttcagc
    11281 gggacgctga aacgggaaag cccctcccga ggaaggggcc ataaataagg aaagggtcat
    11341 gatgaagcta ctcatcatcg tggtgctctt agtcattagc tggccagctt actaaggcta
    11401 ccagggcggg ggaaaccccg ctctaccctc actcctgaaa gtatgccttc acgataagat
    11461 tgtcaatctg caggctttgc agtctgcgat cctgccagca aatattcttt gcgagacgtt
    11521 acgcaataat cacactggaa actattttat ccgcaagtca gcgatagact gcatacaggg
    11581 cgaaaggagg taagccgacg atttcagcgg gacgctgaaa cgggaaagcc cctcccgagg
    11641 aaggggcctt aataaggaaa gggtcatgat gaagctactc atcatcgtgg tgctcttagt
    11701 cataagcttc cccgcttact aagactacca gggcggggga aaccccgctc taccctcact
    11761 cctgaaatta tgccttcacg ataagattgt caatccgcag gctttgtagt ctgcgatcct
    11821 gccagcaaat attctttgcg agtcgttacg caataatcac agatgaaact attttgttca
    11881 cgcgttagcg atagactgca ttcagggcga aaggaggtaa gccgacgatt tcagcgggac
    11941 gctgaaacgg gaaaccctct cccggagaag agggctttta ataaggaaag ggttatgatg
    12001 aagcacgtca tcatactggt gatactctta gtgattagct tccaggctta ctaagaacac
    12061 cagggggagg gggaaacctc tccctaaccc tcactcctga aattatgtca ctccgccctt
    12121 agtgtcaatc gacggctctc cgttcttgcg aggcgaatag caaaattttc tgcgccgacg
    12181 ggcctaattc tgaaagtttc ttcgtttgct cttatttcat tattgcaatc ggtatgatac
    12241 cggacctcga taattctcta agaaaggatt tcttatgagc gaacaaatca cgtttgccac
    12301 cagcgatttt gccagtaacc cggaaccacg ttgcccctgc attttgctgc tggatgtttc
    12361 tggctcgatg agcggcagac ctattaatga acttaacgcc ggattggtta cctttcgtga
    12421 tgaactgctt gctgatccgc tggctttaaa aagagtggaa ctcggcattg tgacgttcgg
    12481 cccggtgcat gtggaacaac cctttaccag cgccgctaac tttttcccgc ccatcctgtt
    12541 tgcccagggg gatacgccta tgggggccgc cattaccaaa gccctggata tggttgaaga
    12601 gaggaaacgt gagtaccgcg caaatggtat ttcgtattat cgcccgtgga ttttcctgat
    12661 caccgatggc gctccaaccg atgagtggca ggctgccgcc aacaaagtgt ttcaggggga
    12721 agaagataag aagtttgcct tcttttccat cggtgttcag ggggcggata tgaagactct
    12781 ggcacaaatc agcgttcgtc agcctttgcc actgcaggga ttacaattcc gtgaactgtt
    12841 tagctggtta tccagttcgc tgcgttcggt ttcccgctcc acgccgggaa cggaagttgt
    12901 gctggaagcg ccaaaaggct ggacgtcagt gtgagctggc gtctggttta tgcatccgct
    12961 gttggaacat cgcacattag tgctgatttg ccttgtcagg atgcctgcca gatgcaggtt
    13021 gtctggttaa acgaccagca accattactg gtgatgttcc ttgctgatgg cgcaggtagc
    13081 gtttcgcagg gaggagaagg cgcgacgctc gccgtcaatg aagcgatggc gtttatggtg
    13141 caaaaagtac aggacggtga attggggctt aatgatgtcc tcgccacaaa tatggtactg
    13201 actattcgtc aacgactttt tgccgaggca gaagccaaag cattagcagt tcgcgatttt
    13261 gcctgcacgt ttctgggatt gatttcatcg cctgacggca ccttgattat gcagattggc
    13321 gatggcggcg tggtggtaga tttgggtcac ggtctgcaat tgcctctcac gccgatggtg
    13381 ggtgaatatg ccaacatgac gcacttcatt accgacgaag atgccgtttc cagactggaa
    13441 actttcacca gcactgggcg tgcgcataaa gttgcagcat ttacggatgg tattcagcgg
    13501 ctggcgttaa atatgctgga taattctcct catgtgcctt tctttacccc gtttttcaat
    13561 ggactggcgt cagcaacgca ggaacaactc gatttattac ctgaattgtt aaagcagttt
    13621 ttgtccagcc cggcggtaaa cgagcgtacg gatgatgata aaacgttggc actggcgctg
    13681 tggttaccat gaaacccact ttatatactg ctactggtga gtgcgttacg ccaggccgtg
    13741 aactgggcaa aggcggtgaa ggcgcggttt atgatatcaa tgagtttgtc gatagcgtcg
    13801 ccaagattta tcacacgccg ccacccgcct taaaacagga caaacttgcc tttatggctg
    13861 cgacagctga cgcgcaattg ttgaattatg tcgcctggcc gcaggcaacg cttcacggtg
    13921 gacgaggcgg aaaagttatc ggttttatga tgccaaaagt ttctggtaaa gaaccgattc
    13981 atatgatcta tagcccggca catcgtcgtc aacgttaccc tcattgtgcg tgggattttc
    14041 tactctatgt tgcgcgcaat attgcttcat cttttgctac ggttcacgag cacgggcacg
    14101 tcgtgggtga cgtaaaccag aacagtttta tggtaggtcg cgacagcaaa gtggtgttga
    14161 ttgatagcga ctcctttcag attaacgcca atggcacact gcatttatgc gaagtcggcg
    14221 tgtcgcattt tacgccgcca gagctacaaa ccttgtcgtc atttgtcggc tttgaacgca
    14281 cagaaaatca cgataatttt ggccttgcgt tgctgatttt tcacgtcttg tttggtggcc
    14341 ggcatcctta ttctggtgtg ccgcttatct ctgatgcggg taatgcgctg gagacggata
    14401 ttacccattt ccgttatgcc tacgcgtcag ataatcagcg acgtggttta aaaccgccgc
    14461 cacgatcgat tccgctatcg atgttaccga gtgatgttga agccatgttt cagcaggcgt
    14521 ttacggaaag tggcgtggca accgggcgtc cgacggctaa agcgtgggta tcagcactgg
    14581 attctctacg ccaacagtta aagaaatgta ccgtttcggc aatgcatgtt tatcccggtc
    14641 atttggctga ctgcccatgg tgtgcgctgg ataatcaagg tgttatctat tttattgatc
    14701 tcggcgaaga ggtcattacc accggcgggg attttgtgct ggcaaaagtc tgggcgatgg
    14761 tgatggcgtc agtagcaccg ccagcattgc aactgccatt acccgatcat ttccaaccga
    14821 ctggcaggcc gcttccttta ggcctgttac ggcgcgaata catcattctg cttgagatcg
    14881 cactgtcagc attatcgctg ctgctttgcg gccttcaggc agaaccgcgt tatattattt
    14941 tggttcctgt gctggcggct atctggatta ttggcagttt gacaagcaaa gcgtacaaag
    15001 cagaagttca gcaacgccgt gaggcattta atcgtgcgaa aatggactat gaccatttag
    15061 tccgccagat ccagcaggta ggcggcctgg aaggttttat cgccaaacgg acgatgctcg
    15121 aaaaaatgaa ggacgaaatt ctcgggttac cggaagaaga gaaacgcgct ctggcagcac
    15181 ttcatgacac tgcaagggaa cggcagaagc agaagtttct ggagggattt tttattgatg
    15241 ttgcctctat tcccggcgtt ggccctgcgc gtaaagcggc gttacggtct tttggtattg
    15301 aaacagccgc agatgttacc cgtcgtggcg ttaagcaagt gaaagggttt ggcgaccatc
    15361 tgacccaggc ggttatcgac tggaaagcga actgtgaacg ccgttttgtg ttcaggccga
    15421 atgaagcgat aacgccagca gacagacaag cggtaatggc gaaaatgacc gccaaacgac
    15481 atcggctgga atcggcgttg acagtcggcg cgacagagtt gcagcgattc cgccttcatg
    15541 ctccagcacg gaccatgccg ttgatggaac cgttacgtca ggcagcagaa aaactggctc
    15601 aggcgcaggc agatttaagc cgctgctgag ccatttttca attaacgaaa caccacttcc
    15661 gcccagcgtg ccagcccggc ggtgacggag ccaaagtcat cgccgcctgc aatcggaatg
    15721 cccggcaact gttctgccag cgctttttta atctgcggag aacgggcgct accgccggtc
    15781 aggtagataa cgtccggttt ttcctgggcg ttatccaggg ccagttgcac ctgttccaga
    15841 atccgcgcca gtggctggct gagggcgctt tccagccctc gctggctaat cagcgtagcc
    15901 agttcattgc tgataaacgg cagtgaggcg cgggtttccg ctacgcttga aagagcaatt
    15961 ttgcactctt ctgcgctacg caccaggcga tagcttaaac gctgacgcca gactttctgt
    16021 aacagggcca ctttttccgg ttcgcgggca tcgcgtacca gatcgttaag cagacgaccg
    16081 ttggcactac tgtagaaatc actctgcgca ggtacgtcgt tgatggcaac cgcattccac
    16141 cacggcagga tcggcagggc gatgcctttt tcggtttcgc cacccatacc cagcagtggc
    16201 atcaggtttt taaacgccag ggcgatatcc agatcgttac cgccaatacg acaaccactg
    16261 tgacccagca gactggcttc acgatcgaga cgcgaacgcc actgcggccc catcagcagc
    16321 aatgaacagt cagtcgtacc accgccgata tccaccacca gcacccgttt ttcttcctgc
    16381 aaggtggctt cgtaatccag cccagccgcg atcggctcgt actggaatac cacgtccctg
    16441 aatccggcac gcttcgccgc gcgttccaga atctcttgcg cctgggcgtt tgcttcatcg
    16501 ccgcccagcc cctggaagtt gatcgggcga ccaatcaccg cctgagtaat cgcttctggc
    16561 agctgcgcct gcgcctgctg gcgaatgtgc aacatcattg cgcagaccag atcctcaaac
    16621 agcgctacct gctgcggttt taagccgctg gcaccgagga acgattttgg tgatttcaca
    16681 aaccacactt cttctggatc atcaatgtac tgtgccagtg aggaaagacc gaactgcacg
    16741 cttttcgccg taacatcgat atcttcttcg cggttataac gaatcgcccg acgtaacagc
    16801 gcctgcgttt catcgtcgtc tgccggaaca tcatgatggc ggtacagcca ttcgcttacc
    16861 gattcacgcg ttggcgcgca aagcattgaa ggcagcagcg tgctgtcgtt ttccattttc
    16921 agtaattgcg gtttaccgtc acgcatgacc gccactgaac agtttgctgt accgtaatca
    16981 aaaccaataa acacgaatta atccccatgc cggtgaagaa ggggcgtgac tttagcgaaa
    17041 tgttgctgtc gcgacaaccg gaatatgaaa gcaaagcgta acgtctgaat aacgtttatg
    17101 ctgaaagcgg attaacaagg agatgcgatg tataccctga actggcagcc gccgtatgac
    17161 tggtcgtgga tgttgggatt tctcgccgcc cgtgcggtga gcggtgtgga aacggtcgcg
    17221 gacaattatt atgcccgtag tctggcggtg ggcgaatatc gcggcgtagt gactgctatt
    17281 ccggatatag cccgccatac tctgcacata aatttaagtg caggtttaga acctgttgcc
    17341 gcagagtgtc tggcgaaaat gagccgcctg cttgatctgc aatgtaaccc gcagattgtt
    17401 aacggtgcgt tgggaaagtt aggcgcgggg cggcccggat tgcgtttacc cggctgtatt
    17461 gatgcttttg agcagggtgt gcgggcgatt ttaggccaac tggtgagcgt ggcgatggcg
    17521 gcaaaattga ctgccaaagt ggtacagctt tatggcgaac ggctggatga ttttccggag
    17581 tatatctgct tccccacgcc tcagcggctg gcagcagccg acccgcaggc attaaaagcg
    17641 ttaggcatgc cgttgaaacg ggcagaggca ctgattcatc tggcaaatgc ggcgctggag
    17701 ggcagcttac caatgacaat tccgggcgat gtggagcagg cgatgaaaac gctgcaaact
    17761 tttccgggta tcgggcgctg gacggcgaat tattttgctt tgcgtggctg gcaggcgaaa
    17821 gatgtttttc tgccggatga ttatctgatt aaacggcgat ttccgggaat gacaccggca
    17881 caaatccgcc gttatgccga gcgctggaag ccctggcgtt cttatgcgct gttgcatatc
    17941 tggtatacgg aaggctggca accagacgaa gcatgacgtt ggtcgccgga tgcggtgcga
    18001 gcgccttatc cggtctgggg aatgagcgat ttgtaggcat gataagacgc gttagcgtcg
    18061 catcaggcaa tgggcaaacg ctctctcagt taatcgcgaa ataactgcta ttcaccagca
    18121 aatccagcgg ttgggcatcg gcaatcacat caccataaat cagatcgacg ccgatgccag
    18181 aaagcgtatc catcactaag ggtaaaacga ctggcccggc gatggttttc atcccgagtc
    18241 gctgagcgtg cccctgaata atcgtaatca gcatctcatc catcaaatta ccctgcacgt
    18301 tggcgcataa ctcaccatca agtagcaggt aatctgccat attcgctttc agcgagttga
    18361 agatttgcag atcgcggccc acctggctga gcactatccg acatcccgcc agctgcagtt
    18421 tttgcacgct ttctgcgtga tctaaaatcg cttcagccgg aataatcaga tgtaataacc
    18481 gtggtggtag agggctattt tccagctgct caagcagatc attcaccagc gtggcgctac
    18541 tcaaaccggc aacggaaagg gggagggcga tgcttatgcc tttactggca accgctttgg
    18601 cggcctgctg gaaaaattcg tggaataccc gtcggtcaag agcatggcta agtgccggat
    18661 cgctgaagct acgacgaaat gtttgttcat caataatttc gccttcgcaa ctccagagct
    18721 taagcgaaat cagccacaaa ttacgcgctt ccgggatccg cggcgaagcg acaccgtggg
    18781 cgatcatcat caactgattc tctttaatca tccgccactg ttcatcaagc gacatcgccg
    18841 cccgttcgct atgtgcggca gcttgctgtg gttcgtaaac cgtcacccgg ccccgtccac
    18901 catttttgga ggcataacag gcgatatcag cctgcgacat cacttctgcc gcctgatgat
    18961 tgttgtcatc gatcaaggta atcccggcgc tggcacctac ccgatgtacc cggccttccc
    19021 atataaagtg atagtcattc acagcactga taatacgtgt agcgataaaa cgagcgcttt
    19081 caacattgca gtctggcagc agcagaccaa attcatcacc gccgagccgc gccagcacgt
    19141 cactggagcg cagcatactc agcatcaacg acgccagttc gcgcagcaaa gcgtcgcccg
    19201 ccgcatgccc ggcgctgtca ttcaccgctt taaagcgatc aagatcgata aacaccaggg
    19261 catgtcgctg atgcgtactg tttaccgttt gcagcaggat acgcagttgt ttctcaaaac
    19321 tggcgcggtt ggcgagatgc gtcagtgcat catgggaggc gctgtagctc agctggcgca
    19381 gcattttgcg tgattcggtg acgtcctgaa tcaccagaac cgaaccaata ttgctgccgt
    19441 ccagagtact taacggcgta atactgtaat gcacgtcgta actgccgccg ctccgacagt
    19501 gcaacaccac atcttgttcg attgcggaac gtgaggtgtc ggcactgtaa atgttctcca
    19561 ttaatggtcc gttgtcgcca aaagtaatat gcaacaccgt caggagcgga acacctaacg
    19621 cttcttcctg cgtccagccg ctcatcttct ccgccactgg attcataaag gtaattttca
    19681 tcgccatatc aatacagacc acggcttcac cgatggaatc aagcgtaatg tgcaggcgct
    19741 ctttttcctg aaacaatgcc tcgttaagct gtttcacttc ggtcatatcc atattgatgc
    19801 cgaggagacg ttcgacttcg ccttctttat tcagtacccg gttggcgagg gcgcggatat
    19861 ggcgaatacc gtcttttacg gtaatgcgaa attccagttt aaagggcgag cgtgattgca
    19921 acgaatcacg aatcactttt tcggcatgct ggcgatcttc cggcagcacg cactcgtacc
    19981 acacctgcca gttcggtttg atatgtggag gaatttcata cagctcgaac atccgcttat
    20041 cccagctaaa aacattcggc ttcaactccc actcccagat gccaatcccg cccgcttcgt
    20101 tagccagcgt gatgcgctcc atcagttgct gattgacctg ttcggtgcgt tttagctcgt
    20161 taatgtcttc aatctgagcg ataaaataga gcggcgtgcc atccgtgtgg cgcaccagtg
    20221 agacggcaag caacgcccag acaacatcgc cattgcggtt gtagtagcgt ttttccattg
    20281 aataggtgtt tatttcaccg cttatcagct tttcaacctg ttggagatct ttattgagat
    20341 cctccggcca ggtcagttgc tgaaaggtga gtccgcgcag ctcttcctga ctgtacccga
    20401 gaaactggca gagcgctttg ttggtttgca gccattgtcc ctcggtgccc actaacgcca
    20461 taccgatagc ggaatattcc atcgcgttcc gaaagcgggt ttcgctttcg gaaatgtgtt
    20521 tgcgttccgc acgaaaggca tacatcacca tggtcatgat gttggcgggc agcaggatca
    20581 gcaaaaacgg tagccacggc atatggctca tcaggtacgt acgcggcgta gcaagcaggg
    20641 agggatccgc ggccatcatc agcgacacca tcatcaccgt ggtaaggaag atcaaaaagg
    20701 cttccattcg tggcaggcgc acggcgctcc acatcaacag cacaataatg aaagtaaaag
    20761 gccacggcag atacagcatc gaaagccagc ttaacgtcag tgtgatggct aacgtgagca
    20821 gcgactcaaa aagcaaccgt gggttgcgat ggcgcagcag atagtgtggt ttaaataaca
    20881 atcccagcgg caccagtgcc agagcgccga tggattctga cagtacccat atcaaaaatg
    20941 ccctgagagg atcgtctccg ggcgtcagca ggacaaccag aacacccccc aacagaggcg
    21001 gaacaatggc gctgccgagt gccagacgca gccagtcagc cagattttgc aaggggttat
    21061 accacggcag caatttacgt agcagcactg ccccgaccac ggcttcaaca atattgatgg
    21121 tcgtccaggt catgttcagc gagctggtgg aaaaaagcag gatggatgcg gcgatatttc
    21181 ccagcgaaca gctcagcgca attcccggcc acatgcgccc ggcatggcga taaaacgcca
    21241 ccatcatgat ggacgtcggg aaccacagtg gggcgagttg ggtgccaaac tgcgaaagct
    21301 caagcgagaa aagggtaaag ataaacgaga ctaaacctaa actgaccagg tgaagcagcg
    21361 ggtggggcag ggcaattaat acatgctgtg attgtttgct cattaccgct ttatccgcaa
    21421 gaccaggtcg ccagtattcg tttctgtgtt tatgctagta caaaagtttt acattttata
    21481 tgcgaattgc tcataaagtg acgtaaaggc ggataatttg cgcaactgcg tataacattt
    21541 tttaccttac ataaaactga tcaacgtaat ttgccaccgg gaattctttg tcaacaatta
    21601 actctgagcg cgcggcaacg ctattcgact ggtatcagac ggatgaaatc cctataattg
    21661 ccgcgtttgg cgcttcgtcg cccccttcct aacatccagg ttaatcaggt cgctaaatat
    21721 atgactgatc agtctcatca gtgcgtcatt atcggtatcg ctggcgcatc ggcttccggc
    21781 aagagtctta ttgccagtac cctttatcgt gaattacgtg agcaagtcgg tgatgaacac
    21841 atcggcgtaa ttcccgaaga ctgctattac aaagatcaaa gccatctgtc gatggaagaa
    21901 cgcgttaaga ccaactacga ccatcccagc gcgatggatc acagcctgct gcttgagcat
    21961 ttacaagcgt tgaaacgcgg ctcggcaatt gacctgccgg tttacagcta tgttgaacat
    22021 acgcgtatga aagaaacggt gacggttgag ccgaagaagg tcatcattct cgaaggcatt
    22081 ttgttgctga cggatgcgcg tttgcgtgac gaacttaact tctccatttt cgttgatacc
    22141 ccgctggata tctgcctgat gcgccgcatc aagcgtgacg ttaacgagcg tgggcgttca
    22201 atggattccg tgatggcgca atatcaaaaa accgtgcgcc cgatgttcct gcaattcatt
    22261 gagccttcta aacaatatgc ggacattatc gtgccgcgcg gcgggaaaaa ccgcatcgcg
    22321 atcgatatat tgaaagcgaa aataagtcag ttctttgaat aagcttgata aattgtgtac
    22381 cgttcagtga taacctggta tgcccttgac gtaattggcg ttaagggagt gatgcgcgaa
    22441 aggagaaaat gccatgcgtc tgtgtgaccg agatattgaa gcctggcttg atgaaggccg
    22501 tttgtcgatc aacccacgtc cgccagtgga gcgtattaac ggcgcgacgg tggatgtacg
    22561 cctgggcaat aaatttcgta ccttccgtgg tcacacggca gcgtttatcg atctgagcgg
    22621 tcccaaagat gaagtgagcg ccgcgcttga ccgcgtgatg agcgatgaga tcgttctcga
    22681 cgagggcgag gcgttctatc ttcacccagg agagctggcg ctggcggtga cgctggagtc
    22741 ggtgacgctg ccagccgatc tggtgggctg gctggacggg cgttcctctt tggcgcgtct
    22801 ggggctgatg gtgcacgtca ccgcgcaccg tatcgatccg ggctggtctg gttgcattgt
    22861 gctggagttc tacaactccg gtaagctgcc gctggcgctg cgtccgggca tgttaattgg
    22921 tgcgctgagc tttgagccgc tttccggccc ggcggcgcga ccttacaacc gccgtgaaga
    22981 tgcgaaatat cgcaaccagc agggcgcggt agccagccga atcgataaag actaattcag
    23041 gcccattgag gataccatga gacgatttct gacgacgctg atgatactcc tggtcgtgct
    23101 ggtggccggg ttatctgcgt tagtgttgct ggtgaatccg aatgatttcc gcgactatat
    23161 ggtcaagcaa gttgctgcac gtagcggtta tcaattgcag ctcgacgggc cactgcgttg
    23221 gcacgtctgg ccgcagctta gtatcctctc cgggcgaatg tctctcaccg cccagggcgc
    23281 aagccagcca ctggttcgcg ccgacaacat gcgtctggac gtggcgcttt taccactact
    23341 gagtcatcaa ctgagcgtta agcaggtgat gctaaaaggg gcagtgatcc aactgacgcc
    23401 gcagacggaa gcggtgcgca gtgaagacgc tccggttgca ccgcgcgaca ataccttgcc
    23461 ggatctgtca gacgatcgcg gatggtcgtt tgatatatcc agtcttaagg tggcggacag
    23521 cgtgctggtg ttccagcatg aagatgacga gcaggtgaca atccgcaata tccgcctgca
    23581 aatggaacaa gatccccaac atcgtggctc atttgagttc tccgggcggg ttaatcgcga
    23641 tcagcgcgat ctcacaatat cccttaacgg tacggtggat gcttctgatt atccgcatga
    23701 tttaacggcg gctattgaac aaattaactg gcagttgcag ggtgccgatt taccaaaaca
    23761 aggtattcag gggcagggga gtttccaggc ccagtggcag gagtcacata aacgcctttc
    23821 atttaaccaa atcagcttga ccgccaatga cagtacgctg agcgggcaag cacaggtcac
    23881 gctgacagag aaaccggaat ggcagctgag actgcaattc ccgcaactga atcttgacaa
    23941 cctcatcccg cttaatgaaa ccgcgaatgg tgaaaacggt gccgcgcagc aggggcagag
    24001 ccaatcaacg ttgccgcgcc cggtcatttc ttcgcgtatt gatgaaccgg cctatcaggg
    24061 actgcaaggc tttacggctg atattttgtt gcaggccagt aacgtgcgct ggcgcggaat
    24121 gaattttaca gatgttgcca cgcaaatgac caacaagtcg ggtttgctgg aaattactca
    24181 actgcagggc aaacttaacg gtggacaggt ttcactgccg ggcacgctgg acgcgacatc
    24241 aataaatccg cggataaact tccagccacg gctggaaaac gttgagattg gtaccattct
    24301 gaaggcgttt aactatccga tttcgttgac cggaaaaatg tcactggctg gtgatttctc
    24361 cggtgctgac atagatgccg acgcattccg ccacaactgg caaggacagg cacatgtcga
    24421 aatgaccgac acgcgcatgg aagggatgaa cttccagcag atgattcagc aagcggtaga
    24481 gcgtaatggc ggtgatgtga aggccgctga aaactttgat aacgtgacgc ggcttgaccg
    24541 ctttaccacc gatttgacgt tgaaggatgg cgtcgtgacg ttaaacgaca tgcaaggtca
    24601 atcgcctgtg ctggcgctga caggggaagg catgttgaat ctggcagatc aaacctgcga
    24661 tacccagttt gatattcggg tcgtgggtgg ctggaacggg gaaagtaaac tgattgattt
    24721 cctgaaagaa acgccagtac cgctgcgggt ttatggcaac tggcagcaac tcaattacag
    24781 cctgcaagtg gatcagttac tgcgcaaaca tctacaggac gaagcgaaac gtcgcctgaa
    24841 tgactgggcc gagcggaata aagattcccg caatggcaag gatgtgaaga agttgctgga
    24901 gaagatgtag gttaccaggt gaccagtcct gtaggcctga taagacgcgt aaagcgtcgc
    24961 atcaggcgtt cgtggcggca tcattatatt ctgaatatgt aagatcgaag gctgttccgt
    25021 tcacttgatg ttcctcgcta ctatggaacg ctcaagccgg tgaacgtgcc agggtggctc
    25081 aatcgccacc accctggcaa cccgggctcc cggcggtaaa tcgccgctgg cgcggttccc
    25141 tcatcttatt cctttcggct attgggtaaa atgaccgata ccatcacacc tcgtactcca
    25201 actcgccatc cttatgcagc ggaataatct gcaccttctg cacgcgatgg ctttccacct
    25261 gcaacgtttt aagcaaataa tcccccacct gaacttcttc gccaggtttt ggaatacgct
    25321 gtaaatattc catcaacagc ccggcaatgg tgtgatattc acgtttttca tccagcggca
    25381 gcggcacata ttgcaccaga tcttccagcg gcatatgacc attcgccgtc caggaaccgt
    25441 ccgcattctt ctgaatatca tggcgggcgt cgatctcttc cacttcgttc ggtaagttac
    25501 cgacaatggt ttcagtgacg tcacttaatg tcacaatccc ttccaccgag ccaaactcat
    25561 ccaccacaaa agcaaagtgc gtgcgggcat tacggaactg ctccagggca ggtaacaacg
    25621 gcaaggtttc cgggaacacc agcggctggc gaatcaacac ccgcaggttg agcggttcgc
    25681 cgcgcagaga ctgttgtagc aggtcgataa cgtgaacaac accgagcaaa tcttctgcgt
    25741 catcaccgtc ggtaaccacc agccgcgtat gctgatttcg ctccagcaac tggcggattt
    25801 cttcttccgg cgcgttgaga tcgatatgct caatatcgtg gcgcgacgtc ataatgctgc
    25861 tgacggtacg ctggttaaga ttaagtaccc gctcaatcat ccgccgttcc tgcggattaa
    25921 agatctgctg gttaccatga tccaccagca tggacgcggt ttcggcgtcc agttccgcat
    25981 cttctttttg cccgctaagc aggcgcatta ccgcttcggt agtccgctgg cgcagcgtct
    26041 ggttagcgga aagaaaacgt cgccggttaa agatagccaa ctgattgagc gcctcgatca
    26101 tcaccgagaa accaatggca gcgtacaggt agcctttcgg aatgacgaag ccgaaacctt
    26161 ctgccaccag gctaaagcca atcatcaaca ggaagcttaa gcagagaata acgatcgtcg
    26221 ggtgactgtt aacaaattgc gttaacggct tgctggccat caacatcagg ctgatagcga
    26281 taaccacggc ggccatcatg accagtaaat ggtcgaccat cccgacggcg gtaatcactg
    26341 agtcgagtga gaaaatggcg tccagtacca caatttgcgt caccacgccc cagaacttcg
    26401 cacctttgcg ttgtgtggga ttattgctgt ctttcccttc cagccgttcg ttcagctcca
    26461 tcgtggcttt gaacaacagg aagaaaccac caaacagcat gattaagtcg cgggcgctaa
    26521 aggtaaacga gcggaagctg aacagcggtt gagtcagggt gaccagccag gagattgacg
    26581 ccagcagtaa caggcgcatt aacatcgcca gcagcagccc ggtaacccgt gcgcggtcgc
    26641 gctgcctcgg cggtagtttt tcggcgagga tggcaataaa gaccaggtta tcaatgccga
    26701 ggaccagttc gatcacaatc agcgtgatta acccggccca gatagacgga tcggcaatcc
    26761 attccatagt aagtacaaaa cctattcgtt gtatgacaaa tgtcacaatt cgatcatgga
    26821 taaatcgggg caaaaatgca atgttgcagc actattta
//
LOCUS       DAZRUF010000030        26556 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZRUF010000030 DAZRUF010000000
VERSION     DAZRUF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.030"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            49..780
                     /gene="artJ"
                     /locus_tag="ACHXOL_001016"
     CDS             49..780
                     /gene="artJ"
                     /locus_tag="ACHXOL_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR6808063.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(955..2082)
                     /gene="rlmC"
                     /locus_tag="ACHXOL_001017"
     CDS             complement(955..2082)
                     /gene="rlmC"
                     /locus_tag="ACHXOL_001017"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR6808064.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(2123..2611)
                     /locus_tag="ACHXOL_001018"
     CDS             complement(2123..2611)
                     /locus_tag="ACHXOL_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR6808065.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2671..3516)
                     /gene="potI"
                     /locus_tag="ACHXOL_001019"
     CDS             complement(2671..3516)
                     /gene="potI"
                     /locus_tag="ACHXOL_001019"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR6808066.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(3513..4466)
                     /gene="potH"
                     /locus_tag="ACHXOL_001020"
     CDS             complement(3513..4466)
                     /gene="potH"
                     /locus_tag="ACHXOL_001020"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR6808067.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4476..5609)
                     /gene="potG"
                     /locus_tag="ACHXOL_001021"
     CDS             complement(4476..5609)
                     /gene="potG"
                     /locus_tag="ACHXOL_001021"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR6808068.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLNGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5704..6816)
                     /gene="potF"
                     /locus_tag="ACHXOL_001022"
     CDS             complement(5704..6816)
                     /gene="potF"
                     /locus_tag="ACHXOL_001022"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR6808069.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7167..7643)
                     /locus_tag="ACHXOL_001023"
     CDS             complement(7167..7643)
                     /locus_tag="ACHXOL_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR6808070.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7731..8633)
                     /gene="rimK"
                     /locus_tag="ACHXOL_001024"
     CDS             complement(7731..8633)
                     /gene="rimK"
                     /locus_tag="ACHXOL_001024"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR6808071.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8694..9416)
                     /gene="nfsA"
                     /locus_tag="ACHXOL_001025"
     CDS             complement(8694..9416)
                     /gene="nfsA"
                     /locus_tag="ACHXOL_001025"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR6808072.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9400..9687)
                     /locus_tag="ACHXOL_001026"
     CDS             complement(9400..9687)
                     /locus_tag="ACHXOL_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR6808073.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9847..10104
                     /gene="grxA"
                     /locus_tag="ACHXOL_001027"
     CDS             9847..10104
                     /gene="grxA"
                     /locus_tag="ACHXOL_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR6808074.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10134..10511)
                     /locus_tag="ACHXOL_001028"
     CDS             complement(10134..10511)
                     /locus_tag="ACHXOL_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR6808075.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10781..12466
                     /locus_tag="ACHXOL_001029"
     CDS             10781..12466
                     /locus_tag="ACHXOL_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR6808076.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12641..13177)
                     /gene="rcdA"
                     /locus_tag="ACHXOL_001030"
     CDS             complement(12641..13177)
                     /gene="rcdA"
                     /locus_tag="ACHXOL_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR6808077.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13261..14469
                     /locus_tag="ACHXOL_001031"
     CDS             13261..14469
                     /locus_tag="ACHXOL_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6808078.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            14469..15284
                     /gene="ybjI"
                     /locus_tag="ACHXOL_001032"
     CDS             14469..15284
                     /gene="ybjI"
                     /locus_tag="ACHXOL_001032"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR6808079.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            15370..15654
                     /gene="ybjH"
                     /locus_tag="ACHXOL_001033"
     CDS             15370..15654
                     /gene="ybjH"
                     /locus_tag="ACHXOL_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR6808080.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTGDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(15695..16922)
                     /gene="mdfA"
                     /locus_tag="ACHXOL_001034"
                     /pseudo
     CDS             complement(15695..16922)
                     /gene="mdfA"
                     /locus_tag="ACHXOL_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            17207..17803
                     /gene="ybjG"
                     /locus_tag="ACHXOL_001035"
     CDS             17207..17803
                     /gene="ybjG"
                     /locus_tag="ACHXOL_001035"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR6808081.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            17861..18619
                     /gene="deoR"
                     /locus_tag="ACHXOL_001036"
     CDS             17861..18619
                     /gene="deoR"
                     /locus_tag="ACHXOL_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR6808082.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(18666..19868)
                     /gene="dacC"
                     /locus_tag="ACHXOL_001037"
     CDS             complement(18666..19868)
                     /gene="dacC"
                     /locus_tag="ACHXOL_001037"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR6808083.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            20115..20740
                     /gene="gstB"
                     /locus_tag="ACHXOL_001038"
                     /pseudo
     CDS             20115..20740
                     /gene="gstB"
                     /locus_tag="ACHXOL_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(20737..21852)
                     /gene="yliI"
                     /locus_tag="ACHXOL_001039"
     CDS             complement(20737..21852)
                     /gene="yliI"
                     /locus_tag="ACHXOL_001039"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR6808084.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21963..22346)
                     /gene="bssR"
                     /locus_tag="ACHXOL_001040"
     CDS             complement(21963..22346)
                     /gene="bssR"
                     /locus_tag="ACHXOL_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR6808085.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22559..23884
                     /gene="rimO"
                     /locus_tag="ACHXOL_001041"
     CDS             22559..23884
                     /gene="rimO"
                     /locus_tag="ACHXOL_001041"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR6808086.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23931..25252)
                     /locus_tag="ACHXOL_001042"
                     /pseudo
     CDS             complement(23931..25252)
                     /locus_tag="ACHXOL_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(25260..>26556)
                     /locus_tag="ACHXOL_001043"
     CDS             complement(25260..>26556)
                     /locus_tag="ACHXOL_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFR6808087.1"
                     /translation="SIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLA
                     VGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENV
                     QTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFS
                     EFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVS
                     AERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPW
                     QLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISID
                     DFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVE
                     SEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         6736 a         6897 c         6396 g         6527 t
ORIGIN      
        1 ccgcagacac ggccaaaaat cataagattg acagacggga gttccatcat gaaaaagtta
       61 gttcttgccg ctttacttgc ttcctttact ttcggtgctt ctgccgcaga gaaaatcaat
      121 tttggcgttt cagccaccta tccacccttt gaatctatag gtgctaataa tgagattgtc
      181 ggctttgata tcgatctggc aaaagccttg tgcaaacaaa tgcaggcaga atgtactttt
      241 actaatcacg cgttcgacag cctgatcccg tcactgaaat tcagaaaata tgactccgta
      301 atctccggta tggatatcac gcccgagcgt agcaagcagg tatcgtttac cacgccctac
      361 tatgaaaact cagccgtcgt gattgccaaa aaagatacct acaaaacgtt tgccgatctg
      421 aaaggcaaac gtattgggat ggaaaacggc accacgcacc agaaatatat tcaggatcag
      481 caccccgaag tgaaaactgt ctcctatgac agttatcaga atgcctttat cgatctgaaa
      541 aatggtcgta ttgatggggt atttggtgac acagcggtgg taaacgaatg gctgaaaacc
      601 aatccgcaac tgggcgttgc tactgaaaaa gtgaccgatc cgcaatactt cggcaccggt
      661 ctgggcatcg ctgtacgtcc ggataacaaa gctctgctgg aaaaactgaa taacgcgctg
      721 gcagcaatta aagctgacgg tacatatcag aaaatcaatg accagtggtt cccgcagtaa
      781 ttgagactga tgacaaaagt aagattgcct gatgcgctgc gcttatctgg cccacatcaa
      841 ctctgcaatg tattgattta gcatgatttt gtaggccgga taaggcgtac acgccgcatc
      901 cggcatgaac aaagcgcacc tgatcagcaa tctgaagccg cacctgcggc ttttttattg
      961 cttcaccagc agcgtcatca cttcatagtg cgcggtgtgc gggaacatat caaaaagctg
     1021 tacccgttcg atgcggtaac ctggcagttc gcggatatct ttcgccatgg tttgggcgtt
     1081 acagctggag tagataataa aacgcggtgc catcgttgag agataatcac acagcggttt
     1141 accaatgccg cggcgcggcg ggttaaccag caccagctcc ggcacatccc cctgagcggt
     1201 ggcaaactga gtggagtcca gcgcctgaaa ttgcaaacgc gttaagccca gttcagcggc
     1261 tgactgcttt gcacaggcaa tggcctctga tgcaatttcg atcccggtta actgcatgtc
     1321 aggcgtcgcg cagtgtaaac caaagccacc cacaccgcag aacagatccc acatatgttt
     1381 aactggcagc tgccgtaccc agtcgcgcgc ggtagcgtac agctggctgg cgaccgccgg
     1441 attagtctgg aagaaacttt gcggacggat ccacagcggt acgtcattaa aacgctctgc
     1501 cagcgcctgt tgttcagtca ggtagatctc cgtctcccct tccataatcg ccatatgtac
     1561 cggctgaata ttgacggtaa taactttcag ttgcggtagt tgttcctgta accacggcag
     1621 cgccttacgc agttgtgcca gtttggtttc agaacgcagt acaaagcgca gcatcatgcc
     1681 gccatcgctc tggctttcag tcagcagaat gtatttcagt tcgccacgct tacgcgccac
     1741 gttgtagggc gttaaccccg cacgggcgat aaacggtttt agcgccgcaa aaacgggcgc
     1801 aaaagaggca ggataaagcg ggcagtcaca aaggtcttcc ggcgtgccat cgcgatgcag
     1861 cataccgagc agtggttttt caacgctacc actcaccacc attttggctt tattacgaaa
     1921 cccttgttcc gggcctgaca ccggcgcgca ccattcctca accggaaaat cggcgagcag
     1981 atttttaaga tcggcggttt tagcggagag ttgctctgga atcggctgcg ttatccactg
     2041 acaggaacga cagcgacccg cgtcgtaaag tgcgcactgc atacattgac cttcacatca
     2101 tcaggggcga cgattataca cattattgca actggaagaa ccgccgactg gtcgagggaa
     2161 cgaacagcag catcaggatg agcatatccg gcagcttctg cagcatcagg ctatggaaga
     2221 tttcacgttt tgattcaccg ggaatgctga acagctccgg ataaccgtac cccagcgaag
     2281 ccgcccacaa gtaacttgcg gcggtgattt gtgtcagcag atatagccag cgcgcccagc
     2341 gacgcccttt caccagtgaa aacgcacacc agatctcaat gaaaaccagc accagactgc
     2401 ttaaaaagac cagcgttaaa ctccaggttt gtacgctgcg atgaatgaac tcgccaatac
     2461 cgcgcacgcc cagcgtattg aaaatcatca gcacgtcgag gccacggatc ataataatgg
     2521 cgagcgccgc cacctgcacc agcgcaggca cattcaggcg agcatgagat gaagatgttt
     2581 tcttaaaaaa tcccaacgtt tcgtcttcca tgaaaacaat gccgcggcat gcgcggcatt
     2641 atgtagccag gttggcaaat tttagtgtct tcagccatgt cttgcacgct ggatatcgcg
     2701 tatccgctgt ttttctgcgc gagccatcag ataccaggcg ataaatccga caattccgac
     2761 cgcaccgaga attaacgttg caagggcgtt gatttccgga ttcactccca tccgcacgct
     2821 ggaaaagacc agcatcggta acgtggtggc tcccggcccg gaaacaaagc tggcgatcac
     2881 cagatcatca agcgacaaag taaaagccag taaccagcca gaaatgatcg cgggcatgat
     2941 catcggtagc gtaatgacga aaaacacttt cagcggcgtc gcaccgagat ccatcgctgc
     3001 ttcttctatc gagcgatcca gttcccgcaa tcttgaagaa ataacgaccg ccacataagc
     3061 cgtacaaaaa gtgacatgcg ccagccagat ggtgagcata ccgcggtccg caggccagcc
     3121 aatggcatga gcaagcgcga cgaataataa caacagcgac aagcccgtga tgacatctgg
     3181 catcaccagc ggcgcggtga tcataaaggc aaaaccattt gatccgcgaa acctgccaaa
     3241 acgcaccagc accaccgccg caatagtccc gaggatcgcc gccgccgttg ccgcacaggc
     3301 agcaattgtc aggcttaaac caaccgcact catcatcgcg tcatcgcgca ataactcacc
     3361 ataccagcgc gttgaccagc cggcccacac cgtcaccagc ttcgagctgt taaacgaata
     3421 gataaccagc atcagcattg gcgcgtagag aaaggtaaag cccagcagca aaatcacaat
     3481 ccgccagggc gaacgaacta ccggtaaatt attcatccgt gttctcccac gcttttttgc
     3541 tggtgtttgt ggaaccacat tatcggcaca attagcagca gcaacatgat gatcgctacc
     3601 gccgaggcca ccggccagtc gcggttattg aaaaactctt gccataacac gcgcccgatc
     3661 atgatgctgt ccgggccacc gagcagttcc gggatcacaa actcgcccac agccgggata
     3721 aacaccagca tcgatccggc aataatccca cctttagtca gcggcacgat cacagtaaag
     3781 aacgttttca gcggtcgtgc accgagatcc agcgctgctt ccaccagcga ataatcaata
     3841 cgaatcaacg cggtataaat cggcagtacc ataaacggca cgtaagcgta aacaatgccg
     3901 atataaacgg ccagattggt atgcagaatg gtcagcggtt gatcgataac ccccagccac
     3961 agcagaaaat tattcagcac accgttgttt tttaatattc ccatccaggc ataaacgcgg
     4021 atcagaaacg aggtccacga cggcaggatc accagtagta ataaaatatt acgggtcgaa
     4081 ggcttactgt gcgccaccgc ccacgccagc ggatagccga tcagtaaaca gcaaaatgtc
     4141 gaaatcgccg ccacctgtaa cgactggaga taagcatcaa aatagagcgg atcgtcggtc
     4201 agttgcagaa aattaccgag attaagagtg atggaaagtt gcccgtcagc ccactccatc
     4261 agttcggtat aaggtggaat agcgcgcgcc atctctccca ggcttatttt aaagacaatc
     4321 agaaatggca gcagaaacag caagatcaac cagatatata gcaacgcaat gaccagtttg
     4381 cgcccatgct tcatttgcag ctgcgacaac cacagcttaa atccgcccgg cggtttcgac
     4441 tgggcagcag gttcaagtgt actcatcttt gctccttaaa ccgtcagcac cacacagctg
     4501 tccacttccc agcacaaacg cacttcgtcg ccccaggtcg gtaacccttt acgatgacga
     4561 tgggcgtttt gtagctgggc gctgatcatc tgcccacttt tcagtcgaac gtgatacacc
     4621 gaaagatcgc cgagataggc aatgtgtatc acctccccca ccgcgaagtt acaaccattg
     4681 gcgggcggct cttcgcaaag catgattttt tccgggcgca gcgctacgtg taccggcacg
     4741 ttatcgacca ctgaagcatc cgcatcgact ttcagtggat gcaccagccc cggcgaatca
     4801 agcaccaggc catcttcctg acgctctttg agtacacctt caaagacatt aaccgaaccg
     4861 ataaattcag cgctgtagcg ggtagttgga tgctcgtaga tctcttccgg ctcgccaatc
     4921 tggacaaatt tcccgcgatt cataatggcg atacgccccg ccatggtcat cgcctcttcc
     4981 tgatcgtggg tgaccatcac acaagtcaca ccgacgcgct ccagaatatc caccacttca
     5041 agctgcatcc ggtcacgcag ctttttatcc agcgcgccca tcggctcatc gagcagtaat
     5101 agtttcgggc gtttggcaag gcttcgggcc agggccacac gctgtcgctg accaccggaa
     5161 agctgatgcg gtttgcgttt ggcgaactcc tgcatgtgca ccaggccgag catctcatgg
     5221 acccgactgg caatttccgc tttcggtagt ttgtcctgtt tcaggccaaa agcgatgttc
     5281 tgttccacgg tcatatgcgg aaacagcgcg taagactgaa acatcatatt gatggggcgc
     5341 aggtaaggcg gcacctgtga caaatcgacg ccattgagca ttatctgccc ggcagaaggt
     5401 tgttcaaaac ctgccagcat acgcagcagc gtggacttgc cacagccgga tgcgcccagc
     5461 agcgcgaaga tttcaccttt gtagatggtc aggctgacat catcgaccgc atgctgacca
     5521 tcgtaggatt tggtgaggtt gcggatttct aatagcggcg tcagcgcctt acgggttttc
     5581 gcctgcgggc gagggatagc gtcattcacg gctgctctcc ggcattgaat gtgtgcaagc
     5641 gcaccatgat ggtgcgaatt gccggctggt gtggtgcgcc cgcccggcat ctaaggctgc
     5701 ggattatttt ccgctcttca ctttggtcca cgcgcgggtg cgcacacggt cgattttcgg
     5761 atcctgtact ttcagagtga atagcttcgc acgaacgtcc gccggaggat aaatacccgg
     5821 gttctcacgg acttccgcac tcaccagcgg cgtggctgct ttattggcgt tggcatagaa
     5881 cacatggtcg gaaatatgcg ctactacatc cgggcgcagc aggtaattca ggaactgata
     5941 ggcttcgtct ttgtttttgg catccgcagg catggcgaat acatcaaaga acgccatcgc
     6001 cccttctttt ggaatcgaga acgagacatt cactccattc ttcgcttcct tcgcgcggtt
     6061 tgacgcctgc cagacatcac ctgcccagcc gatagcgacg caaatatcgc cgtttgccag
     6121 gtcgttaatg tattgagatg aatggaaata acgaatgttc gggcgcagct ttaacagcag
     6181 atcggttgcc ggtccggtgt aatcatccgc tttagtgctg ttgggatctt tgccgagata
     6241 attcaacacg gtagcaaaaa cttcttctgg cgcatccagg aaagagacac cgcagctttt
     6301 cagtttttcc agattttcag gtttgaggat caagtcccag ctatcgacgg gcgcgttttc
     6361 gcccagcaca gctttaactt tatcaacgtt atagccaatc ccggtcgtcg cccacatata
     6421 gggcatagca aatttattgt cgggatcgtg tttggcgacc agcttcagca gctccgggtc
     6481 gagattcttc cactccggca atttgctttt gtccagcggc tggaaaactc ccgcagtcaa
     6541 ctggcgttca agaaagctgg cagatggaac caccaagtca aagccggtgc tacccgccat
     6601 taatttgcct tccagtactt cgttagagtc gaaaacatcg tagacgactt taataccggt
     6661 ttctttttca aaattggcca ccgtgtccgg ggcgatataa tcagaccagt tataaatgtg
     6721 gagtgttttt tgttcagccg cgagcgtgcc gacagagacg gccatcagag cacccgcagc
     6781 cagacccgaa agccattttt tatttaaggc ggtcatatct ctttccttct gaaagttcgt
     6841 taacaaacga acaaaagttg tcacactaaa agatatgcaa taaacgtgca tattttttat
     6901 cggcggggtg taagaaaaga gaactctctc ccggccagca ctgctcgcta tataaaagca
     6961 tcgcaagaat aactttagcc agggtttgag actatagctc ggattaaaaa acggtattta
     7021 acaaattttt atgcgtattt gctctatgtg ataaagccaa atcgacacaa gagaagtgaa
     7081 ataatcttta agaaaaggtt aaaaacagca gataattttg cagcgcgcag ccactgcacc
     7141 agcgactgcg tgctcgaaag gagttatcag tggaggaaat agttttcggt aacgttgttt
     7201 tgcccttcat cgtcagtcgg taacagcagt tgatgggcat tagcttcaag aatgaccatc
     7261 gaaatctgct cttcgctctg acgcacgaac catgcaaact gctcataggt tacgccctgc
     7321 ataacggata aagactggca aaccaccagc tttggcagat tatcatcctg catatcaaga
     7381 aatgctttca cggtcagcga actggcattg atggcagata aatccgccgc cagcggcaat
     7441 acggctgaag gtcggacttc cgccatggca gaaaacagga tcgtgttatc gatcagatcg
     7501 attttggcat caaagacacc gtcgaaattc tgcatatggg gcagatgcag agcctgacag
     7561 ttatcacatt caaaaaaact catccccagg tcatcgagcc attgacgcag cgtatccaga
     7621 ccaggaacga ccagcgatgt cataattttg tacaacctct ttcgataaaa agaccggcac
     7681 agcttacgca aaaagcgcag gcaaaaccat gatcagtaat gtgattgcga ctaaccaccc
     7741 gttttcaggc aatattctgt cgtagcgtgg cgttcgatcc agcggatcat tttacccgcg
     7801 atgtcgatac cagtggtttt ttctattcct tccagccccg gcgacgcatt cacttccatc
     7861 accaacggcc cgcgattagc acgcagaata tccacaccag caacgtccag cgccatcgtt
     7921 cgcgcggctt ttatcgcgat ttcacgctcc tgtggtgtga tactggcgac gcttgccgcg
     7981 ccgccacgat gcaaattgga acgaaaatcg ccctctttcg cccgccgttc aatcgcagcg
     8041 accacttcat cgccaacaac cagacagcgg atatcgcacc cttgcgcctc tttgatatat
     8101 tcctgcacca gaatatgcgc gttcagaccg cggaaagcgt caatcacgct ttccgccgcc
     8161 tgacgcgtct ccgccagcac gacgccaatt ccctgcgtgc cttcaaccaa cttgaccacc
     8221 agcggcgcac caccgaccat gtcgattaaa tcgctggtat catccggcga atgcgcaatg
     8281 cccgtgacag gcaggtcgat gccctgacgc gccagcagtt gcatggaacg caatttgtca
     8341 cgcgcccggg caatggcgac cgactcattg agcggatagc tccccagcat ctcgaactgg
     8401 cgcagtgccg ccgtcccata aaaggtaatg gcggtgccaa tacgcgggat cactgcgtca
     8461 aaatggggta acttgcggcc tttgtagtga atagaagacg ccgcaggatt tatgttcatg
     8521 tagcaagaaa gcggatcaag aatttcaacc aggtgaccgc gctgtatagc ggcttcacgc
     8581 agccgcttac acgaatagag cgttccatcc cgggacaata tggcaatttt caccctgcac
     8641 ctctctgtca gacctggtca aaagcccgcg tatcatacat cgacgtggcg gttttagcgc
     8701 gtcgcccaac cctgtttgtg caaataatcc agaataaatg ggcggctttc tttaatgatt
     8761 gttcggcgga tatgatcgct ccaggtatcc cggcgattat tgctgccacg ggtgaggtaa
     8821 tattccgcca gttgctcgtc atactgcgcc agtgcgtctt tatccagcgg ttgatagctg
     8881 ttttcatgca ccaaaatgga ggccggtaaa cgcggcttga gatccggatt atccgcaggc
     8941 cagccaaggc acagcccaaa cagcggcaga acatgctgcg gtaatttcag cagtttcgtc
     9001 accgcttcaa tattattgcg caggccgccg atatataccc cgcccaatcc cagcgattcc
     9061 gctgcggtta atgcattctg cgccatcatt gccgtatcaa cgacaccgag caacagttgt
     9121 tccgccaggc cgagctgagc atccggacag atctgtaaat ggcggttaaa gtcggcacag
     9181 aacacccaga actccgccgc ttgcgctacg tgtttttgcc cgccggtcag cgtcaccagt
     9241 tcttcacgta atgctttgtc ggtaatgcga ataatgctac tacactgcaa aaaactggaa
     9301 ctggacgtcg cacgggcgct gttaataatc gcctcacact gcgcttcgga aatgggttca
     9361 tcagtgaaat ggcgaatgga gcgatggcca caaataagtt caatggttgg cgtcattatc
     9421 tttttctctt tctgaacgtg aatattgcgg tggacggttc atcagctgtg gggcaagacg
     9481 ttttgccacc tgaagaataa ccaccaccgc agcgggaagc atgagcaaaa caccgagaaa
     9541 aatcatcaga atctgcactt ctggccgaga aaatggctca ggcagcgaca gggagtcgct
     9601 taccgacagc agcgccaccg ccagtagcat cattccgata aattccagta tcaacacgcc
     9661 tttaggcaat ttaccgatcg cgcgcatacg cttccctctg caaagtgagc cttcagtcta
     9721 aaacttttca ctgtattgtg tttaacagtt atagctttta gcaattaatg caacaggtta
     9781 aacctacttt cagcgaatac attttagcgt gatcattaca ggcataaatc tatgaggaga
     9841 gaaataatgc aaaccgttat ttttggtcgt tcgggttgcc cttactgtgt gcgtgcaaaa
     9901 gatctggctg agaaattgag caatgaacgc gatgattttc agtatcagta tgtagatatt
     9961 cgtgcggaag ggatcactaa agaagatcta caacaaaagg caggtaaacc cgtagaaacc
    10021 gtgccgcaga tttttgtcga tcagcaacat atcggcggct ataccgattt tgctgcatgg
    10081 gtgaaagaaa atctggacgc ctgatcgtct gacaagccct cgcgttgagg gctttactga
    10141 ttttttctgt gctgtggttt aaacaaacta ctgataaata agaaacacag tgcccccagc
    10201 gcacaccaga acaccgcgct tagtaaccat gccagctctt gccagaatga gcgcgtcggt
    10261 gaaaaaaaca gccgcataat gagcattgaa cagggtgccg ccagcattgc gccaaataga
    10321 ggtttcagga cttctctacg ctgtgaaaag aaactggcaa ctgctccagg aagaatgaaa
    10381 aacagcaagc cgatttcagg atgcccggca gcccgaaaag cgcctttcat gtgcgtcgcc
    10441 agaaaaaggc acaccacaat gaagaggaca aaacagcaga ttgcccccgc ccaacgttgt
    10501 ttatgtttca ctcgttcctc ctgacactgc gtctatcgaa cacatttttc gccagtgtgg
    10561 cgttcagtaa gataaagccg cttcgcattc catgctaata taggccaacg caattcatat
    10621 agccgttgat acctaatgtg attacactag taaaatatat tgttacttta ctatcgttta
    10681 ggtgcgctga atgaatctgc gccctgaatt ctggtaaaaa acattatcgt aaattaccat
    10741 ttctttcaac agcttactag taaacaagaa gttagcctcc gtgaatataa acgtcgccga
    10801 attgttaaat gggaattaca ttctgttatt atttgtggtc ctcgcgcttg ggctatgtct
    10861 cggaaagtta cgacttggtt cgatccaact gggtaattcc attggcgttt tagtcgtatc
    10921 gctgttatta ggccaacaac atttcagcat taacaccgat gcgcttaatc ttggctttat
    10981 gctgtttatt ttctgcgtcg gggtcgaagc cggaccgaac tttttttcca ttttttttcg
    11041 cgatgggaaa aattacctaa tgttagcact ggtgatggtt ggcagtgcgc tggtgatcgc
    11101 cttagggtta ggtaagctgt ttggctggga tattggcctg acggccggta tgttagcagg
    11161 ctctatgacg tcgacaccgg ttctggtcgg tgctggcgat acactgcgtc attccggcat
    11221 ggaaagcagg cagctctcac tggcactgga taatctgagc ctcgggtatg ccttaaccta
    11281 tttaatcggt ctggtgagtt tgattgttgg tgcgcgttac ttgccgaaat tgcagcatca
    11341 ggacttacag accagcgccc agcaaatcgc ccgcgaacgt ggcctggaca ctgatgccaa
    11401 ccgtaaggtt tatttaccgg tgatccgcgc ctatcgcgtc ggcccggagc tggtggcctg
    11461 gaccgacggc aaaaatctgc gtgaactggg tatttatcga caaaccggct gctacattga
    11521 acgtattcga cgtaacggga ttctggcaaa tccagacggt gatgccgtgc tacaaatggg
    11581 cgatgaaata gcgttggtag gctatcccga cgcccatgcc cgactcgatc ccagcttccg
    11641 taacggtaaa gaagttttcg atcgtgacct tctcgacatg cgtatcgtca ctgaagaagt
    11701 ggtcgttaaa aaccataacg ctgtaggtaa acgtctcgca caactgaagt tgaccgatca
    11761 cggttgcttc cttaaccgcg tcattcgtag ccagattgag atgccgatag atgacaacgt
    11821 cgtgcttaac aaaggtgacg ttttacaagt cagcggtgat gcccgtcgcg taaaaaccat
    11881 cgccgatcgc atcggcttta tctcgattca cagccaggtc actgacctgc tggcattttg
    11941 cgccttcttt gttattgggc tgatgatcgg gatgatcacc ttccagttca gcacattcag
    12001 tttcggcatg gggaacgctg ccgggttgtt attcgccgga attatgctgg gctttatgcg
    12061 tgctaaccac ccgaccttcg gttacattcc gcaaggtgca ttaagcatgg tgaaagagtt
    12121 cggcttgatg gtgtttatgg caggcgttgg tctgagcgcc ggtagcggta ttaataacgg
    12181 cctgggcgcg attggcggtc agatgttgat tgccggatta attgtcagtc ttgtgcccgt
    12241 ggttatctgt ttcttgttcg gtgcttatgt attgcgaatg aaccgcgcac tgttgttcgg
    12301 cgcaatgatg ggcgcacgca cctgcgcgcc ggcaatggag atcatcagtg atacagctcg
    12361 cagtaacatc cctgcgctgg gctatgcggg cacctacgca atcgccaacg tcctgctgac
    12421 gctggcaggg acaatcatcg tcatggtatg gccaggatta ggataaaact gaagttgccc
    12481 tgaaaatgaa atttttttgc acaaccgcag aacttttccg cagggcatca gtcttaatta
    12541 gtgccactgc ttttctttga tgtccccatt ttgtggagcc catcaacccc gccatttcgg
    12601 ttcaaggttg atgggttttt tgttatctaa aatttatcta ctaccctgca accctctcaa
    12661 ccatcctcaa aatctcctcg cgcgatagcg gcttacggtc ggtgacaaaa tgcagcgtca
    12721 tcccctcaat aaacgcatca agcgcgcggg cggttccggg ttcaaaccat tgttcgagcg
    12781 tttgctgact gcgctgcatc cagttttgca ttaccgtttt taatagcggt tttcggctag
    12841 ccagcgcgta gagctggtac atcagctcca tattatccgg cgttgcaacc tgtgagctgt
    12901 agatcatatc ggtgatagcc tggcatgcgc cttgagcatc actaacatcg ctaaaaaatg
    12961 cctgatattg ccgggacata atctcagtaa aactgctgaa cgcctccagc aacaactcat
    13021 caattcctga aaaatagtag gtcatcgatc ccaacggtac cccggcaagg gtagcaattt
    13081 tgcggtgcgt aacagcatgt attccgtaaa gtttcaccgc ctccagcgtg gcctggataa
    13141 ttttttctcg ccgttgcgga tcgttagcgc gacgcatgtc atttcctctg tctgcaattg
    13201 tgtacaaatg tacacaacct tgctaatgtt gtcttccctc ttcttatttt gacgtgatct
    13261 atgaccgtaa attcttcacg taatgcattg aaacgccgaa cctgggcgct gtttatgttc
    13321 ttctttttgc caggcctgtt aatggcgtcc tgggcaaccc gtacgcctgc tatccgcgat
    13381 attctctctg tctcgatcgc tgaaatgggc ggtgttctct ttggtctgtc gatcggttcg
    13441 atgagcggta ttctctgctc ggcgtggtta gtgaaacgct ttgggacacg taatgtcatc
    13501 ctggtcacga tgtcctgcgc attgatcggg atgatgatat taagtctggc actctggctg
    13561 acatcgcccc tgctctttgc cgttggtctc ggcgtctttg gggcaagttt tggttctgcg
    13621 gaagtggcga taaacgttga aggtgccgcc gttgagcgag aaatgaataa aacggttttg
    13681 ccgatgatgc acggttttta tagcctaggc acgctggcag gcgctggtgt cgggatggca
    13741 ctgacggcct ttggcgttcc ggcaacggtg cacattttat tggcggcgct ggtaggtatc
    13801 gcgcctattt atatcgccat tcaggcaatc cctgacggta cgggcaaaaa tgctgccgat
    13861 ggcacccagc atggcgaaaa aggcattcct ttttatcgcg atatccagtt gctgttgatt
    13921 ggtgttgtag tgctggcgat ggcctttgcc gaaggttctg ccaacgactg gttaccctta
    13981 ttaatggttg acggtcacgg ttttagccct acttccggct cgctgattta tgccggtttt
    14041 accctaggga tgaccgttgg acgcttcact ggcggttggt tcatcgaccg ttacagtcgc
    14101 gttgccgtgg ttcgggccag tgcactaatg ggggcgttgg gtattgggct gattattttt
    14161 gtcgatagcg cctgggtcgc tggggtgtct gttgtactct ggggactggg tgcctcgctg
    14221 ggcttcccgc tgaccatttc tgccgccagc gataccggcc ccgatgcacc aacccgcgtc
    14281 agtgtggtag caacgaccgg ttatctggct ttcctcgtcg ggccgccgct gctgggctat
    14341 ctcggcgaac attatggatt acgtagtgca atgctggttg tactggcgct ggttattctc
    14401 gcggctattg tcgcgaaagc cgtcgccaaa cccgatacca aaacgcggac ggcgatggag
    14461 aatagttgat gagcattaaa ttaattgcgg tagacatgga tggtactttc ttaagcgatc
    14521 aaaaaaccta taaccgtgag cggtttatgg ctcagtatca gcaaatgaaa gcacaaggca
    14581 ttcgctttgt ggtcgccagc gggaatcaat attatcagtt gctctctttc ttccctgaaa
    14641 ttgctaatga aattgccttt gtggctgaaa acggcggctg ggtagtgagt gaaggcaaag
    14701 atgtttttaa tggcgagctg tcgaaggatg cctttactac tgtcgtggaa catttgctga
    14761 cgcgcccgga agtggaaatt attgcctgcg gaaaaaatag cgcctatacg ctcaaaaaat
    14821 atgacgatgc catgaaaacg gtggcagaaa tgtattatca ccgtctggaa tacgtcgata
    14881 actttgacaa cttagaagat attttcttta agtttggcct gaatctttcc gatgaactga
    14941 ttccacaagt acaaaaagca ttacatgagg ccatcggcga tattatggtg ccagtccaca
    15001 ccggcaacgg cagcatcgat ctgattatcc ccggcgtaca taaagccaat ggccttcgcc
    15061 aactgcagaa attatgggga atagacgaca gcgaagtggt ggtctttggc gatggcggta
    15121 acgatattga gatgctgcgt caggcaggct ttagttttgc aatggaaaat gccggcagcg
    15181 cggtcgtcgc agcggcaaaa taccgggcag gctccaataa ccgtgaaggc gtactggatg
    15241 tgatcgataa agtgcttaaa cacgaagcgc catttgacca ataaaaacgg cagcacgccc
    15301 ggcaacacaa tacctgaatg aaggctgcat aattcgcgga aattaacccg ctgcattatt
    15361 tcaggcgaaa tgattatgaa aaattgtcta ctgttgggcg cgcttttaat gggctttact
    15421 ggtgtggcga tggcgcaaag tgtcaccggc gatgtgccga gtggatacaa agtggttgta
    15481 gttcctgatt cagtcagcgt tccacaagcg gtcagcgtcg cgactgtgcc gcaaaccgtc
    15541 tatgtggctc ctgcgccagc tccggcttac cgcccgcatc cttatgctcg ccatcttgcc
    15601 agcgtaggtg aagggatggt tatcgaacac cagattgacg accatcacca ttaattcgag
    15661 aatgcctgat cgcacaaagc agtcaggcat ttttttaccc ttcgtgagaa tttcccatct
    15721 gtttatcttt taaaaagata accatcagcg acagccacaa aattccgttg acaaggttga
    15781 agagattaaa ctgtccgttg cccccgttca gccaggcatg tttgctgatt tcaataccaa
    15841 cggtaaagat cagcatttgc agcattccca tcgccgcaga aaccgtacct ttactcatat
    15901 cgctggcaaa cagggttaat cgcaccagtc ccgcattcgc cagaccaata ccgaaagcat
    15961 aaatacttaa cccggcagtc atccataaat acgcgtgcga tgagataacc gttgccgcag
    16021 cagcgaccaa cagaccaatc ataatcggcc agccgcccat aattatcacg tacggtgcgg
    16081 cgcgaggtca gacgcgctaa cagcaagtta cccgcaatta acgccccgaa aataggcact
    16141 tgcagcaagc catattcata gctgctcaac tgctccccgg taatgatgat aatcggcgac
    16201 tgggcgatcc acgccagcaa tggcaggcta acgaatccca gcgccagcgc ccccgccaca
    16261 aagcggccgt tcttcagcac cagcttatag tcacgaccga gttctttcag tgacagtttc
    16321 tcgcctatac gcgtggcggt ttcaggcata gctcgttgca gaccgaaaaa ggagatcgct
    16381 gccaatgcgg caaacaaaac aaacatcccc tcccagggca gcacatggat ccacgccgcg
    16441 cccaccagcg gaccaagtag cggagcaatc agcgccacgt tcgccatcag cgcggtgatc
    16501 ttgatacaaa ccgcctcttc gaaggattcc tgaattgcgg cgtatcccac agcgccaatg
    16561 aaacagaggc ttatgccctg caagaagcgc aacagggtga attgttcaat gttttgcgcc
    16621 agtaatattg ccagacaggt gacgataaac cacaccacac ccgccagcat caccggacgg
    16681 cgaccaatac gatccgacag cggccccagg agccattgta aaaacatccc gcccgccaga
    16741 tacgcggtca tcgaagtagg aacccaatca atgcccgcct gatattgttc caccacggcc
    16801 aacataccgg gttggatcat atcgttgccg atataggttg aaaattcgta aagcaccaga
    16861 cagagaggga aaagtaacgc ctgacgtcca agcctggcac cgaaagctaa tttattttgc
    16921 atgcaatttc ttcgccaata ataatcgcgc agagtttaat aaaagcgcag ctaacgagaa
    16981 agcgaatttt gtagctgaaa ccacggttaa gcacattctt acattattgc gagtatagct
    17041 acgctttctt taagttttat ttaacctatg cccgttacaa tcacccaccg taaacaggcc
    17101 gcttgaggga aataagacga tgccgcttta cccagtttaa cctgcacttt attctcaacg
    17161 acttgcctgt attggctccc ttttaatcac tttgcgtcgg gaagttatgc tggaaaattt
    17221 gaatctctct ctattctctc ttattaacgc gacgccagac tcggctccgt ggatgatctc
    17281 gttggcgatt tttattgcta aagatttgat taccgtggtg ccgttgctgg ccgcggtact
    17341 ttggttgtgg gggcttacag cacaacggca actggtgata aaaatcgcta tcgcgctggc
    17401 ggtcagcctg tttgtgtcct ggacgatggg acatcttttt ccgcacgacc gaccctttgt
    17461 cgaaaatatc ggctataact tcctgcatca tgcggcggat gactcattcc cgagcgatca
    17521 cggtacggtg attttcacct ttgcactggc atttttatgc tggcatcgcc tgtggtccgg
    17581 ctcactttta atggttctgg ccgtcgtcat tgcctggtcg cgcgtttatc ttggcgtcca
    17641 ctggccgctg gatatgctcg gtggattgct ggcaggtatg attggctgcc ttagtgccca
    17701 gattatctgg caagcgatgg ggcataaact ctatcaacgt ctgcaatcgt ggtatcgcgt
    17761 ctgttttgca ttaccgatcc gcaaaggctg ggtgcgtgac tgatctctgc taaaaagtgt
    17821 agtattgagc ggctcgcttc aataactatt cagagggatt atggaaacac gtcgcgaaga
    17881 gcgtatcggg cagctgctgc aagaattaaa acgcagcgat aagttacatc ttaaagacgc
    17941 cgccgccctg cttggggttt cggagatgac gattcgtcgc gatctgaaca accacagtgc
    18001 gcccgtcgtt ttgctcggcg gctatattgt tctggaaccg cgcagtgcca gccattacct
    18061 gttaagcgat caaaaatccc gcctggtgga agaaaaacgc cgggcggcaa aactggctgc
    18121 gacactggta gaacccgatc agaccctctt ttttgactgt ggcaccacca cgccgtggat
    18181 tattgaagcg attgataatg aaatcccttt taccgccgtt tgttattcgc taaatacctt
    18241 tctggcgctg aaagagaaac cccattgccg cgcatttctg tgcggtggtg aatttcacgc
    18301 cagcaacgcc attttcaacc ccatcgattt tcagcaaacg ttgaataatt tttgcccgga
    18361 tatcgctttt tattccgcgg cgggcgtgca tgtcagtaaa ggcgctacct gttttaatct
    18421 tgaagagttg ccggtaaaac actgggccat gtcgatggcg caaaagcatg tgctggttgt
    18481 cgaccacagt aaatttggca aggtgcgtcc ggcgcgcatg ggtgacctga aacgctttga
    18541 tattgtggtg agtgattgtt gcccggaaga tgagtatgtg aagtacgcgc agacgcaccg
    18601 tattaagttg atgtattaat gacgtataac cggatgacgt ttcgcgccat ccggttatca
    18661 gaagattaag agaaccagct gccgaaccac tgatggaatt tcatcatcac gaaatcccac
    18721 atccgaccaa agaatccgcc ctcttccaca ttttccatca cgatcagcgg acgctgctca
    18781 atggatttac cgttaagctg gaaatcaatg gtcccgacaa cctgaccttt tttcagcggt
    18841 gcggtaagct gcggttccgt taacgtgtag ctcgccttca ggtttttcag ctgcccacgc
    18901 ggtatggtca cggagcccgc ttcgcctgcc ccgagattca cttcgctctt atcaccaaac
    18961 cagacgcgct gagtcacaaa ggtggcatca ggtttaattg gcgtcacagt ttcaaagaag
    19021 cggaaacccc aggtcaataa tttctcagac tcattaaaac ggatacggtc agttttcgcc
    19081 cccagcacta cggagattaa acgcatatca ccctgggtag ccgaagcaac cagattatat
    19141 cccgcacctg ccgtggttcc tgtcttcatg ccatcaacat tcagattgct gctccacagc
    19201 agacggttac ggttaggctg acgaattttg ttgaaggtga actctttctc tttatgaatg
    19261 gcgtactctt ccggcacatc gtggatcaat gctttaccca gcaatgccat atcgcgcgcg
    19321 gtactgaact gccccggcgc atccagaccg tgcaccgtct ggaaggtagt gttggtcaga
    19381 cccagttttt tggcataacc attcatcaaa ccaataaatg actcctggct cccggcaacg
    19441 taatcggcca gcgcaataca ggcgtcatta ccggactgga taatcacacc tttgttcaag
    19501 tctgccaccg aaacctgatc gcccggtttg aggaacatta ccgatgaacc acgcagtgcc
    19561 ggatttcccg tcgcccaggc atctttaccg accgtcacca tatcggtgag tttaatctta
    19621 tcggccttaa gcgcctgccc aaccacatag ctggtcatga ttttagtcag gctcgcggga
    19681 tccagtttct catccgcgtt gccttctgcc agcactttac cgctggcgta atccattaaa
    19741 atccatgcac gcgcatccac gctcggcgct tcaacggttt gttccgccgc gaatgccgtt
    19801 ggggcaaaaa ggaataaaaa tgcagaaccc gctgcaagac cacgaaggag agaggagtat
    19861 tgcgtcataa atgccacccg agtatccatt caaaaaaaat acgttatatc gccgtatgat
    19921 aagttcggtg attaataacg cacacacaaa cttaaagaaa ccgtaagttg gtaaagtttt
    19981 taaagtttat gcaatatcct gccgcttcgc tgcttctggt gtcatttttt tgccagctgt
    20041 tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt cagcctgtgg ctgcctgtcg
    20101 gaaaaggggt gagtatgatt acgctgtggg gtcggaataa ttcaactaac gtaaaaaagt
    20161 attgctgacg ctcgaagaac tggaactacc ttatgagcaa attctcgcgg gccgtgagtt
    20221 tgggataaat cacgatgctg attttctggc gatgaaccct aacgggctgg tgccgttgtt
    20281 gcgtgacgac gaaagtgatc tcattctttg ggaatcaaac gccattgtcc gctatctggc
    20341 ggcacagtac gggcaaaaac gcctgtggat cgactcaccg gcacgtcgtg cggaagcgga
    20401 aaaatggatg gactgggcaa accagacgct cagtaatgct catcgcggga tcctgatggg
    20461 attagtcaga acaccaccgg aagagcgcga tcaggccgcc attgatgcca gttgcaaaga
    20521 gtgcgacgcc ctgtttgccc tgctcgatgc ggaactggca aaagtaaaat ggttctctgg
    20581 cgacgagttt ggtgtgggcg atatcgctat cgcaccgttc atctacaatt tgtttaacgt
    20641 tggcctgacc tggacaccgc gtccaaatct gcaacgctgg tatcagcaac tcactgaacg
    20701 ccccgcagtg cgcaaagtgg tgatgattcc cgttagctaa ttgcgtgggc taactttaag
    20761 taattcccca ctggactcgt cggtgagaac gtataaataa ccgtcgggtc cagtgcgaac
    20821 atcacgaatt cgctgccctc tgtccgttaa aatacggcca tcttctgtca ctttgtcgcc
    20881 gttgacgctc atcacaatga catctttatc tttcagcgcg ccaataaata atttttgctg
    20941 ccactggggg aatttatcgc tgttatagaa ggccatgccg ctcacagcgg gcgaatcttt
    21001 ccagtaaaaa acaggttgct cggtcccggc gacgatctcc cctttcgctt ccggtatctt
    21061 aaagcctgaa tagttgattc cccaggttgc cagcggccag ccgtagtttt tgcctttttg
    21121 cgggatatta atttcatcac caccgcgcgg gccatgttca ttcagccaca gtgcattact
    21181 ccacggattc atcgccattc cttgcggatt acgaatgcca taagaccaga tctcggcgcg
    21241 cgcaccggat tcctttataa aaggattatc atccgggatt tcgccctggt cggtcagacg
    21301 caccagtttg ccctgtaatt tatccagatc ctgcgccgtc gggcgctgat tgttttcgcc
    21361 cagagcaata aaaagataac ctttaccgtc gaataccagc cgcccgccaa aatggttgcc
    21421 ggtagacagt tttggcatct ggcggaagac ggtgcggaag tcggtcactt ttgagagatc
    21481 atcacttaag cggccataac ccacagcagt tccggctttg ccatcatcgc caacttcgga
    21541 ataacttaac cagatgcggc gagactgagc aaaatcaggc gctaaaacca cgtccagcag
    21601 gccgccctgc ccgtgcgccc aaacgtccgg aactccggaa agcggcgcag ataatccttt
    21661 tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg attaacatac cgtgattatc
    21721 gggtaaaaag gccagtgccc agggatggtc gagtttgtct tgcagtactt cgacatttac
    21781 cgttgcagga gccgcccaga gagcggaaga aagacaaata aggggcacaa ggaaaaagga
    21841 ttgtcgatgc ataacgcctc cggattgact ctggcttaaa gcgtagtcag tggaggagat
    21901 aacaaattca tttttacaaa aacttaaaca tgaaggggga gacgctttct cccccttagt
    21961 tttcaggcct tctcaagcat ggcgtgcttc tgcaggctct ggatactcag cgttaagctc
    22021 atcagacaat tttcaagctt atcggcgttg acggtaataa cagtcgggca atcatggtgc
    22081 ccactcatca aacatactgc ggctgtcgct aatgcttctt cagcatgatg aagagcactc
    22141 cactcttcct gatccagatg aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc
    22201 agtttaaaaa agttatctcg tagatgattg ctttcgctga cggacatgta tccttttgcc
    22261 ttcctcagtt gcacgtatgc agcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga
    22321 tcgattcgct gtctgtcaac gaacatactt cgatcctcct ctttccagcc ttgttgcgat
    22381 taaatagtat ggctattttt tgagcaaaat gctgtccagg atcaattctt cacgccaaat
    22441 taacaaaatt tacatttcct tcgcctccgc cagcgaatga gattgcgata caaattttat
    22501 ccagaactct gtagaatccc tgccctgacc tttcctgtaa ctgaatattt tttaagctat
    22561 gagcaaagta actccccagc cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct
    22621 tgtcgattca gagcgtattc tcaccgaact acgcactgaa ggttatgacg tggtaccgag
    22681 ctatgacgat gcggacatgg tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca
    22741 agaatcactg gaagccattg gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtgaccgg
    22801 ttgtctgggg gcaaaagaag atcagatccg cgaagtccac ccgaaagtgc tggaaatcac
    22861 cgggcctcat agctatgagc aggttctgga gcacgttcat cactacgtgc caaaaccgaa
    22921 acacaaccca ttcctgagcc tggtgccaga acaaggtgtg aaactgacac cgcgtcatta
    22981 tgcctatctg aaaatttcgg aaggctgtaa tcaccgctgc accttctgca ttattccgtc
    23041 tatgcgcggc gacctggtga gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct
    23101 ggtagatgcg ggcgttaaag agattctggt gatctcgcag gatacttccg cctatggcgt
    23161 tgatgttaaa catcgtactg gcttccacaa cggcgagccg gtaaaaacca gcatggtcag
    23221 cctgtgcgaa cagttatcga aactggggat ctggacacgt ctgcactacg tttaccctta
    23281 tccgcatgtg gacgacgtca tcccactgat ggcagaaggc aaaatcctgc cgtatctgga
    23341 cattccgttg cagcacgcca gcccgcgcat tctcaaactg atgaagcgtc cgggttctgt
    23401 agatcgccaa ctggcgcgca tcaaacagtg gcgcgaaatc tgcccggaac tgaccctacg
    23461 ctcaaccttt attgtcggct tccctagcga gacggaagaa gatttccaga tgctactcga
    23521 cttcctgaaa gaagcgcgtc tggatcgcgt tggctgcttt aaatacagcc cggttgaagg
    23581 tgcagacgcc aatgccctgc ctgaccaggt tccggaagaa gtgaaagaag aacgctggaa
    23641 ccgtttcatg cagctgcagc agcagatttc cgccgagcgc ctgcaagaga aagtgggccg
    23701 tgaaattctg gtgattatcg acgaagtgga cgaagaaggc gcgattggtc gcagcatggc
    23761 agatgcaccg gaaatcgacg gcgcggttta tctcaatggt gaaaccaacg ttaagccggg
    23821 tgatatcctg cgtgtgaaag tcgagcacgc cgatgagtac gatttgtggg gtagccgggt
    23881 ttaattaatt caggccgggc aaaatcattt gtccgccctg cgaataatga ttactgccag
    23941 ctaatctgag ccctgccgtt tttcttactc ttatacagcc gctcatccgc cagcattttc
    24001 aagcgttcga cggtggtaca ttcatttgaa tgcgctacac cgatactggt acttaaatta
    24061 atgcttccca cctggaaatg aacgatttct tttagaatcg atagcaactc ttccagcgtt
    24121 ccacccgtat aaatcaccgc aaattcgtca ccgccgaagc ggtagatttt acctttatct
    24181 accgggatct gtgatttaag gattttcacc actttcatca acacttcatc gcccagcaaa
    24241 tgaccgaagg tgtcgttaat gtttttgaat ttatcgacgt caaacaggca caaataaccg
    24301 cctttttccg gcagcgcttt taaatcttcg tcaaaggatt ttctgcccat tgctttggtc
    24361 aggtcgtcca ggaacgccga gttatacata ctgtagagtt ttttgacatc ggcagccgaa
    24421 aagaaaagcc cgccatccag tacgcgttca aaatggttat taatgatggc ttctttctcc
    24481 acaataccac gattaatcac tctgcgtacc agatagaggc aactggtacc cgatatcact
    24541 gccaccaatg cggcaaagaa aataatcaat gtacagaccc gcaaataaac atctttttta
    24601 tacgcaaata cgtacaatcc ggtcggcaaa taggcgaagt gctccatcgc ttccgaaacg
    24661 atgaagatgg cgtttttagc attatattca tcaccggttt accgttctta aatagagaga
    24721 acatcaggtt ctcgctgtcc atcgccagcg attttgacag taaagaaaaa cgctccagac
    24781 caagcaaaaa tatcagacta cgattgtgag ttaccggata gaggtaatag acgaaggttt
    24841 taccgtcaaa agagataaac cctgtatttt tgacatgtcg atacttcaca acctgcataa
    24901 tatctgcaca ataatttttc tcgaactcct ttacctgaat acattcgtca ctggaaatca
    24961 gataaggacc gacatcgtac aaataagagg gggaaaccga gtcggcgtaa gctgtgcgca
    25021 aaccaaccaa cagctgttcc tccgtgggat ttagcgtgtt gataagatca attgccacgc
    25081 tacgccgata ttcagcaata tttgccaccg caaaagcagt atcaataacg gactgtttca
    25141 ggctatacac ccgttccttt accatttgag tgtagatccc gcaggcaact gctgaaatca
    25201 tgataaaaat cagcagactg actgtaagta cgaacttatt gattctggac atacgtaaac
    25261 tactctttta ctaattttcc acttttatcc caggcggaga atggcatggg tttctggtac
    25321 agatacccct gagcctgaac acatcctaat ttaattaatt cgcgcgcaac gtcttcgctc
    25381 tctacgcctt ccgcaatcac cgggatccgg ttatagcgtg ccagatcgaa aataatgcgc
    25441 acaatttttc ccgcagtacc gaaaatatcg ccggtaaatt gcgagtccac ctttatgctg
    25501 tctggattaa tttcataaaa acgtttcagg tttgacaaac cggtgccaaa gtcatcaatc
    25561 gatatccgta ccccttgttt acgcagttgt tcaatattga gataaaaatg ttcattaatt
    25621 cgctcgatca cggtttcagt gatctcaaca cagaggctgt tcgccttcag gccattttct
    25681 ttcatgatgg tggtaaaacg ctcatgaaag tgcggttcgt ttaactgcca gggcgaaaca
    25741 ttaatatgca gtaagaaatc atccttcagc gacgcacgct gactaaaatg tctgaattca
    25801 cccattgccg ttttaataat ctgataaccc agctcattga taaacccgat atcttcagca
    25861 atggtaatga acttcagcgg tgaaatgatc ccccgctccg cagaaaccca acggcacaac
    25921 gcctccgcgc ccagaatggc gcgggtgtta atatcaacga tcggttgata gacgaggaat
    25981 agttcgccaa tttccattgc atggcgaata ttacgcgcaa tccagatatt ctcttctatt
    26041 tcgttgagtt tcgcgtcatc acagaaaata aactcggaga acatatggtc acggatactg
    26101 gaaatcgcca gtttcagttt ttctgcgtaa ctccaggcgt tttccccctg caaaggttca
    26161 caaatggcag acttatggat gcgcagattg cggtagccaa agctgctgtc aaaatcgttt
    26221 accatcgcta ttttacgggt aaatgtctgt acattttccc gacacagaag taaatagaga
    26281 tcggcattga atgtcacgat atcgcagcat tgcgcatatt tctcggcaat tcgctgacac
    26341 aatttcctga cgattttatt gcgttcatca acaccaaaca ccgcttccac ggcttccaga
    26401 ccacacagtt tgatgatccc cacagccaga tactgattat tgttggactc aataatcgcc
    26461 atatttttaa taaaaccgct ttcctgatcg taagagatca gtgagagcaa tttatttgac
    26521 cgttcaattt cggcatcttt catttccgcg atcgaa
//
LOCUS       DAZRUF010000031        26161 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZRUF010000031 DAZRUF010000000
VERSION     DAZRUF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.031"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(84..1334)
                     /gene="icd"
                     /locus_tag="ACHXOL_001044"
     CDS             complement(84..1334)
                     /gene="icd"
                     /locus_tag="ACHXOL_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR6808088.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1506..2159
                     /gene="rluE"
                     /locus_tag="ACHXOL_001045"
     CDS             1506..2159
                     /gene="rluE"
                     /locus_tag="ACHXOL_001045"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR6808089.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2169..2630
                     /gene="nudJ"
                     /locus_tag="ACHXOL_001046"
     CDS             2169..2630
                     /gene="nudJ"
                     /locus_tag="ACHXOL_001046"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR6808090.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2684..3790
                     /gene="mnmA"
                     /locus_tag="ACHXOL_001047"
                     /gene_synonym="trmU"
     CDS             2684..3790
                     /gene="mnmA"
                     /locus_tag="ACHXOL_001047"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR6808091.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3826..4467
                     /gene="hflD"
                     /locus_tag="ACHXOL_001048"
     CDS             3826..4467
                     /gene="hflD"
                     /locus_tag="ACHXOL_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR6808092.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4471..5841
                     /gene="purB"
                     /locus_tag="ACHXOL_001049"
     CDS             4471..5841
                     /gene="purB"
                     /locus_tag="ACHXOL_001049"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR6808093.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6009..6680
                     /gene="phoP"
                     /locus_tag="ACHXOL_001050"
     CDS             6009..6680
                     /gene="phoP"
                     /locus_tag="ACHXOL_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR6808094.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6680..8140
                     /gene="phoQ"
                     /locus_tag="ACHXOL_001051"
     CDS             6680..8140
                     /gene="phoQ"
                     /locus_tag="ACHXOL_001051"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR6808095.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8216..9337
                     /gene="roxA"
                     /locus_tag="ACHXOL_001052"
                     /gene_synonym="ycfD"
     CDS             8216..9337
                     /gene="roxA"
                     /locus_tag="ACHXOL_001052"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR6808096.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9386..10612)
                     /gene="pepT"
                     /locus_tag="ACHXOL_001053"
     CDS             complement(9386..10612)
                     /gene="pepT"
                     /locus_tag="ACHXOL_001053"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR6808097.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRVAELTAQRK"
     gene            10862..11998
                     /gene="potA"
                     /locus_tag="ACHXOL_001054"
     CDS             10862..11998
                     /gene="potA"
                     /locus_tag="ACHXOL_001054"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR6808098.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11982..12839
                     /gene="potB"
                     /locus_tag="ACHXOL_001055"
     CDS             11982..12839
                     /gene="potB"
                     /locus_tag="ACHXOL_001055"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR6808099.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12836..13630
                     /gene="potC"
                     /locus_tag="ACHXOL_001056"
     CDS             12836..13630
                     /gene="potC"
                     /locus_tag="ACHXOL_001056"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR6808100.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13627..14673
                     /gene="potD"
                     /locus_tag="ACHXOL_001057"
     CDS             13627..14673
                     /gene="potD"
                     /locus_tag="ACHXOL_001057"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR6808101.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(14829..15650)
                     /gene="cobB"
                     /locus_tag="ACHXOL_001058"
     CDS             complement(14829..15650)
                     /gene="cobB"
                     /locus_tag="ACHXOL_001058"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR6808102.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(15666..16577)
                     /gene="nagK"
                     /locus_tag="ACHXOL_001059"
     CDS             complement(15666..16577)
                     /gene="nagK"
                     /locus_tag="ACHXOL_001059"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR6808103.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16606..17850)
                     /gene="lolE"
                     /locus_tag="ACHXOL_001060"
     CDS             complement(16606..17850)
                     /gene="lolE"
                     /locus_tag="ACHXOL_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR6808104.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(17850..18551)
                     /gene="lolD"
                     /locus_tag="ACHXOL_001061"
     CDS             complement(17850..18551)
                     /gene="lolD"
                     /locus_tag="ACHXOL_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR6808105.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18544..19743)
                     /gene="lolC"
                     /locus_tag="ACHXOL_001062"
     CDS             complement(18544..19743)
                     /gene="lolC"
                     /locus_tag="ACHXOL_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR6808106.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20005..21078
                     /locus_tag="ACHXOL_001063"
     CDS             20005..21078
                     /locus_tag="ACHXOL_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR6808107.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21206..24652
                     /gene="mfd"
                     /locus_tag="ACHXOL_001064"
     CDS             21206..24652
                     /gene="mfd"
                     /locus_tag="ACHXOL_001064"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR6808108.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            24796..25752
                     /gene="ldtC"
                     /locus_tag="ACHXOL_001065"
                     /pseudo
     CDS             24796..25752
                     /gene="ldtC"
                     /locus_tag="ACHXOL_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(25835..26092)
                     /gene="bhsA"
                     /locus_tag="ACHXOL_001066"
                     /gene_synonym="ycfR"
     CDS             complement(25835..26092)
                     /gene="bhsA"
                     /locus_tag="ACHXOL_001066"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR6808109.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6346 a         6819 c         6658 g         6338 t
ORIGIN      
        1 ctcgatatgc tattaataat atccacataa ataaaacaac gggcgtgtta tacgcccgtt
       61 tcaatattta actcatggag agattacatg ttttcgatga tcgcttcacc aaactctgaa
      121 catttcagca gcttagcgcc ttccatcaga cgttcaaagt cataagttac ggttttcgcg
      181 ttgatagcac cttccatacc tttaacaatt aaatcagccg cttcagtcca ccccatatgg
      241 cgcagcatca tctcagcgga gaggatgata gagcctggat ttactttgtc ctgaccggca
      301 tatttcggcg cagtaccgtg ggtggcttca aacagggcgc attcgtcacc gatgtttgcg
      361 ccaggggcga taccgatacc gccaacctgc gctgccaggg cgtcagaaat gtagtcaccg
      421 ttcaggttca tacaggcgat aacatcatat tcagccggac gcagcaggat ctgttgcagg
      481 aatgcatcag caatcacgtc tttaatgacg atctctttgc cggtgttcgg gtttttaact
      541 ttcagccacg ggccgccgtc gatcagttca ccgccaaact cttcacgcgc cagctggtag
      601 ccccagtctt taaacgcgcc ttcggtgaac ttcatgatgt tgcctttgtg caccagagtc
      661 acagagtcac gatcgttagc aattgcgtat tcgatcgctg cacgaaccag acgtttggtg
      721 ccttcttccg aacacggctt aataccgata ccacaatgtt ccgggaagcg aattttcttc
      781 acccccatct cttcacgcag gaatttaatc actttctcgg cgtcggcaga gtctgctttc
      841 cattcgatac ccgcataaat gtcttccgag ttttcacgga agataaccat atcggtcagt
      901 tcagggtgtt taaccgggct tggagtgccc tgatagtaac gtaccggacg caggcagatg
      961 tagagatcca gttcctggcg cagggcaacg ttcagagagc gaataccgcc accaacagga
     1021 gtggtcagcg gacctttaat ggcaacgcga tattcacgaa tcagatcaag ggtttcagca
     1081 ggcagccaga catcctgacc ataaacctgt gtggattttt caccggtgta aatttccatc
     1141 caggagattt tacgctcgcc tttataggct ttctcgactg cagcgtcgac cactttcagc
     1201 atggctgggg ttacatctac accgattcca tcaccttcaa tgtaagggat aatcggattt
     1261 tcaggaacgt tgagtttgcc gttttgcagg gtgatcttct tgccttgtgc cggaacaact
     1321 actttacttt ccattcacct ctccttcgag cgctactggt ttgctcgtct gccaccacgt
     1381 tgcatatgcg tttgcgtcct gcgatacgga tgctttagag caattttttg ttaatgattt
     1441 gtaattggct tgtcaatgct accctattgt ttgccgtcat gaaagatgcg cgttattagg
     1501 ctataatgcg gcaattcata atctctgaaa ataccatgca aaaaacttct tttagaaatc
     1561 accaggttaa gcgattcagc tcgcaacgtt ctactaggcg taaacctgaa aaccagccca
     1621 cgcgtgtgat cctgttcaat aaaccctacg atgttcttcc gcagttcacc gatgaagccg
     1681 gacgcaaaac attaaaagaa ttcatcccgg ttcagggcgt ttatgcagca ggtcgtcttg
     1741 accgcgatag cgaagggtta ctggtgctga ccaataacgg cgcgttgcag gcgcgtttaa
     1801 cccagccggg caaacgcacc gggaaaatct attatgtgca ggtggaaggt attcccacac
     1861 aagacgcact tgaagccttg cgcaatggcg taaccttaaa tgatggccct accctgcccg
     1921 ccggcgcgga gctggttgac gaaccagcgt ggttatggcc gcggaatcca ccaattcgtg
     1981 aacgcaaaag tattcccacc agctggctga agatcacctt atatgaagga cgtaatcgcc
     2041 aggtgcgccg catgaccgcc catgttggtt tccccacgct gcgactgatt cgttatgcga
     2101 tgggtgatta cactttggat aaccttgcca atggcgaatg gcgagaagca acagaataag
     2161 gatgcacaat gtttaaaccg cacgttaccg ttgcttgcgt agtgcacgca gagggtaaat
     2221 ttttagtcgt tgaagagacg attaatggta aagcgttatg gaaccaacct gccgggcatc
     2281 tggaagccga tgaaacctta gtggaagctg ccgcccgtga gctatgggaa gaaaccggca
     2341 tcagcgcgca accgcaacac tttattcgta tgcatcagtg gattgcgcca gataaaacgc
     2401 cgtttttgcg tttcctcttt gccattgagc ttgagcaaat atgcccgacc cagcctcatg
     2461 acagcgatat cgactgctgt cgttgggtca gcgccgaaga aattttaaag gcgtcaaatc
     2521 ttcgttcgcc gctggtggcg gaaagtatcc gttgttatca aagtgggcaa cgctatccgc
     2581 tggagatgat tggcgatttt aactggcctt ttacaaaggg tgtcatctaa aaggctgcgt
     2641 gatagaatac gccgccttga agttcaatgt cgtgagtgat ccaatgtctg aaaccgcaaa
     2701 aaaagtaatc gtcggcatgt ccggcggtgt cgattcctcc gtttctgcct ggctgttgca
     2761 acaacaggga tatcaggttg aaggcctgtt tatgaagaac tgggaagaag acgacggtga
     2821 ggaatattgc acagcggcag cggatctggc tgatgcccag gctgtctgcg acaagctcgg
     2881 cattgaactg cacaccgtta actttgctgc cgagtactgg gacaatgtct tcgaactgtt
     2941 ccttgccgaa tataaagccg gtcgcacgcc gaatccggat attctgtgca acaaagagat
     3001 caaatttaaa gctttcctcg aatttgccgc cgaagattta ggtgccgatt atatcgctac
     3061 cggtcattac gtacgtcggg ccgatgtcga tggcaagagc cgcctgctgc gtggtctgga
     3121 cagcaataaa gaccagagct acttccttta tacgctcagc catgagcaga ttgcgcaaag
     3181 cctgttcccg gtcggcgaac tggaaaaacc gcaggtgcgt aagattgctg aagatcttgg
     3241 tctggtcacc gcgaagaaaa aagactctac cggcatttgc ttcattggcg aacgtaaatt
     3301 ccgcgagttc ctgggccgtt atctcccggg gcaaccgggc aaaatcatta ccgtcgatgg
     3361 cgatgaaatt ggtgagcacc aggggctgat gtatcacact ctcggtcagc gtaaaggtct
     3421 gggtatcggt ggcaccaaag aaggcaccga agaaccttgg tatgtggtgg acaaagacgt
     3481 cgaaaacaac attctggttg tcgctcaggg ccatgaacac ccgcggctga tgtctgtcgg
     3541 gttgattgcc cagcagttgc actgggtcga tcgcgaacca ttcaccggca ctatgcgttg
     3601 cacggtaaaa acccgctatc gccagaccga tatcccttgc accgtcaagg cgctggacga
     3661 tgatcgcatt gaagtgattt tcgatgaacc ggttgccgcc gtgacgccgg gccagtctgc
     3721 cgtcttctat aacggtgaag tgtgcctcgg tggcggtatt attgagcagc gtctgccgct
     3781 gccggtctga ttattatctt tacttaaaac aaggaagcag tgaacgtggc aaagaattac
     3841 tatgacatca ccctcgccct ggccggtatt tgtcagtcgg cacgcctggt gcaacaactc
     3901 gctcaccagg ggcattgtga tgccgatgcg ctacacgtct cactcaacag tattattgat
     3961 atgaacccca gctcgacgct ggcggttttt ggcggtagcg aagccaacct gcgcgtcggg
     4021 ctggaaaccc tgctcggcgt gctcaatgcc agcagtcgcc agggcttaaa cgccgaatta
     4081 acccgctaca cactcagctt gatggtgctt gagcgcaaac tctcctcagc gaaaggcgcg
     4141 ctcgacactc tgggcaaccg gatcaacggc ctgcaacgcc agctcgaaca cttcgattta
     4201 cagtccgaaa cgctgatgag cgcgatggct gctatctatg ttgatgtgat cagcccgctt
     4261 ggcccacgca ttcaggtcac cggttcccct gctgtactgc aaagcccaca agtgcaggcg
     4321 aaagttcgcg caaccctgct ggcaggcatt cgcgccgccg tgctctggca ccaggtcggc
     4381 ggcggacgtc tgcaactgat gttttctcgt aatcgcctga ccactcaggc aaaacaaatt
     4441 cttgctcatt taaccccgga gttgtgatct atggaattat cctcactgac cgccgtttcc
     4501 cctgtcgatg gacgctacgg cgataaagtc agcgcgctgc gcgggatttt cagcgaatat
     4561 ggtttgctga aattccgtgt acaagttgaa gtacgttggc tgcaaaaact ggccgcgcac
     4621 gcagcgatca aggaagttcc tgcttttgct gccgacgcaa tcggttacct tgatgcaatt
     4681 gtcgccaatt tcagcgaaga agatgccgca cgcatcaaaa ccatcgagcg taccactaac
     4741 cacgacgtta aagcggttga gtatttcctg aaagaaaaag tggcggagat cccggaactg
     4801 cacgcggttt ctgaattcat ccactttgcc tgtacttcgg aagatatcaa taacctctcc
     4861 cacgcattaa tgctgaaaac cgcgcgtgat gaagtgatcc tgccgtactg gcgtcaactg
     4921 attgatggca ttaaagatct cgccgttcag taccgcgata tcccgctgct gtctcgtacc
     4981 cacggtcagc cagccacgcc gtcaaccatc ggtaaagaga tggcaaacgt cgcctaccgt
     5041 atggagcgcc agtaccgcca gcttaaccag gtggagatcc tcggcaaaat caacggcgcg
     5101 gtcggtaact ataacgccca catcgccgct tacccggaag ttgactggca tcagttcagc
     5161 gaagagttcg tcacctcgct gggtattcaa tggaacccgt acactaccca gattgaaccg
     5221 cacgactaca tcgccgagct gtttgattgc gttgcacgct tcaacaccat tctgatcgac
     5281 tttgatcgtg acgtctgggg ttatatcgcc cttaaccact tcaaacagaa aaccattgct
     5341 ggtgagattg gttcttccac catgccgcat aaagttaacc cgatcgactt cgagaactcc
     5401 gaagggaatc tgggcctttc caacgcggta ttacagcatc tggcaagcaa actgccggtt
     5461 tcccgctggc agcgtgacct gaccgactcc accgtgctgc gtaacctcgg cgtgggtatc
     5521 ggttatgcgc tgattgcgta tcaatccacc ctgaaaggcg tgagcaaact ggaagtgaac
     5581 cgtgaccatc tgctggatga gctggatcac aactgggaag tgctggctga gccaatccag
     5641 acagttatgc gtcgctatgg catcgaaaaa ccgtacgaga agctgaaaga gctgactcgt
     5701 ggtaagcgcg ttgacgccga aggcatgaag cagtttatcg acggtctggc gttgccagaa
     5761 gaagagaaag cccgcctgaa agcgatgacg ccggcaaact acattggtcg cgccatcacc
     5821 atggttgatg agctgaaata aactcgtatc agtgccggat ggcgatgctg tccggcctgc
     5881 ttattaagat tatccgcttt ttattttttc actttacctc ccctccccgc tggtttattt
     5941 aatgtttacc cccataacca cataatcgcg ttacactatt ttaataatta agacagggag
     6001 aaataaaaat gcgcgtactg gttgttgaag acaatgcgtt gttacgtcac caccttaaag
     6061 ttcagattca ggatgctggt catcaggtcg atgatgcaga agatgccaaa gaagccgatt
     6121 attatctcaa tgaacattta ccggatattg cgattgtcga tctcggattg ccagacgagg
     6181 acggtctgtc actgattcgc cgctggcgta gcaatgatgt ttcactgccg attctggtat
     6241 taaccgcccg tgaaagctgg caggacaaag tcgaagtatt aagtgctggt gctgatgatt
     6301 acgtgactaa accatttcat attgaagagg tgatggcgcg aatgcaggca ttaatgcggc
     6361 gtaatagcgg tctggcttca caggtcattt cgctaccacc gtttcaggtt gatctctctc
     6421 gccgtgaatt atctattaat gacgaagtga tcaaactgac cgcgttcgaa tacaccatta
     6481 tggaaacgtt gatacgcaat aatggcaaag tggtgagcaa agattcgtta atgctccaac
     6541 tctatccgga tgcggagctg cgggaaagcc ataccattga tgtactgatg ggacgtctgc
     6601 gcaaaaaaat tcaggcacaa tatccgcaag aagtgattac taccgttcgc ggccagggct
     6661 atttgttcga attgcgctga tgaaaaaatt actgcgtctt tttttcccgc tctcgctgcg
     6721 ggtacgtttt ctgttggcaa cggctgcggt agtactggtg ctttcgcttg cctacggaat
     6781 ggtcgcgctg atcggttata gcgtcagttt cgataaaact acgtttcggc tgttacgtgg
     6841 cgagagcaat ctgttctata cccttgcgaa gtgggaaaac aataagttgc atgtcgagtt
     6901 acccgaaaat atcgacaagc aaagccccac catgacgcta atttatgatg agaacgggca
     6961 gcttttatgg gcgcaacgtg acgtgccctg gctgatgaag atgatccagc ctgactggct
     7021 gaaatcgaat ggttttcatg aaattgaagc ggatgttaac gataccagcc tcttgctgag
     7081 tggagatcat tcgatacagc aacagttgca ggaagtgcgg gaagatgatg acgacgcgga
     7141 gatgacccac tcggtggcag taaacgtcta cccggcaaca tcgcggatgc caaaattaac
     7201 cattgtggtg gtggatacca ttccggtgga gctaaaaagt tcctatatgg tctggagctg
     7261 gtttatctat gtgctctcag ccaatctgct gttagtgatc ccgctgctgt gggtcgccgc
     7321 ctggtggagt ttacgcccca tcgaagccct ggcaaaagaa gtccgcgaac tggaagaaca
     7381 taaccgcgaa ttgctcaatc cagccacaac gcgagaactg accagtctgg tacgaaacct
     7441 gaaccgattg ttaaaaagtg aacgcgaacg ttacgacaaa taccgtacga cgctcaccga
     7501 cctgacccat agtctgaaaa cgccactggc ggtgctgcaa agtacgctgc gttctctgcg
     7561 tagtgaaaag atgagcgtca gtgatgctga gccggtaatg ctggagcaaa tcagccgcat
     7621 ttcacagcaa attggctact acctgcatcg tgccagtatg cgcggcggga cattgctcag
     7681 ccgcgagctg catccggtcg ccccactgct ggacaatctc acctcagcgc tgaacaaagt
     7741 gtatcaacgc aaaggggtca atatctctct cgatatttcg ccagagatca gctttgtcgg
     7801 tgagcagaac gattttgtcg aggtgatggg caacgtgctg gataatgcct gtaaatattg
     7861 cctcgagttt gtcgaaattt ctgcaaggca aaccgacgag catctctata ttgtggtcga
     7921 ggatgatggc cccggtattc cattaagcaa gcgagaggtc attttcgacc gtggtcaacg
     7981 ggttgatact ttacgccctg ggcaaggtgt agggctggcg gtagcccgcg aaatcaccga
     8041 gcaatatgag ggtaaaatcg tcgccggaga gagcatgctg ggcggtgcgc ggatggaggt
     8101 gatttttggt cgccagcatt ctgcgccgaa agatgaataa atatgtccat acttcacgca
     8161 ttacgttaag catccgttat aatcggttgc agataccagc ctgtggatgc ttaacatgga
     8221 ataccaactc actcttaact ggcccgattt tcttgaacgt cactggcaga aacgcccggt
     8281 ggtgttaaaa cgcggcttta ataattttat tgacccgatc tctccagacg agttggcggg
     8341 tctggcgatg gaaagcgaag ttgacagtcg actggtcagt caccaggatg gcaaatggca
     8401 ggtcagccac ggcccgttcg aaagctacga tcatctcggt gaaaccaact ggtcattact
     8461 ggtacaggca gtgaaccact ggcatgagcc gaccgccgcg ctgatgcgac cgttccgtga
     8521 actaccggac tggcgtattg atgatctgat gatttctttt tctgtacctg gcggcggcgt
     8581 cggcccgcat ctcgatcagt acgacgtgtt tatcattcag ggtaccggac gtcgtcgctg
     8641 gcgagtgggc gaaaagctgc aaatgaaaca gcactgccca catccggatc tgttacaggt
     8701 cgatccgttc gaagccatca tcgatgaaga gctggagcct ggtgatattc tttatattcc
     8761 gccaggattc ccgcatgaag gctacgcgct ggaaaatgcg atgaactatt ccgtgggctt
     8821 tcgcgcgcca aatacgcggg aactgattag tggatttgcc gattatgtgc tgcaacgtga
     8881 actgggcggc aactactaca gcgatcctga tgttccacct cgcgctcatc ctgcggatgt
     8941 tctgcctcaa gagatggata aactgcgtga gatgatgctc gaattgatca accagccgga
     9001 acactttaag caatggtttg gcgagtttat atcccagtca cgtcatgaac tggatatcgc
     9061 gccgccagaa ccgccttatc agccagatga aatctacgat gcgctgaaac aaggtgatgt
     9121 gctggtgcgc ctgggtggtc tgcgcgtatt gcgcattggc gacgacgtgt atgccaatgg
     9181 tgagaagatc gattccccgc accgtccggc actggatgca ctcgccagca acattgcgct
     9241 gactgcggag aattttggcg atgcgctgga agatccgtca ttcctcgcga tgctcgcggc
     9301 gctggtcaat agcgggtatt ggttcttcga agggtaagtt tggatttaag ccggatgcgg
     9361 catcacatgc cgcatccctt ttcgcttact tccgttgcgc cgttaactcg gcaacacgga
     9421 cgatcacctg caccgctttt tccatacctt ccagagtcac aaactcatgc ttaccatgat
     9481 agttgtaacc gccagtgaac aagttcgggc acggtaatcc cataaacgac aactgcgcgc
     9541 cgtcggtacc accgcggatc ggtttcagtt ccggttcaat atcgcagtca cgcatcgcct
     9601 gctgggcgat atcgagaata tgcggatgct caaccacttt ctctcgcata ttgtagtaac
     9661 tgtcttcaat caccagttca atgtagcaat caggatgtaa ccctttgccc acttttttgg
     9721 cgatctccat cattttacgt ttacgcgctt caaactgttt acggtcgaaa tcacggatga
     9781 tgtagtgcat atcggcccgt tcaacggtgc ctttcatgct cgccaggtga tagaaacctt
     9841 catagccttc tgtcatttcc gggctttcat ccgccggaac ttccgcatga atacgtgccg
     9901 ccagcgacag cgcatttacc atcactcctt tcgccgtgcc cggatgaaca ttgttaccga
     9961 caattttgat attgaccgat gcggcgttga agttttcaaa ctccagttcg cctacgccac
    10021 caccgtcaac agtgtaagcc cagcgggcat cgaaggcatc aacatcaaaa tgtttcgccc
    10081 ctttgcccac ttcttcatcc ggggtaaagg cgacgcgaat atcaccatgc ggaatgtttt
    10141 tctgttgcaa taccgccagc gcggtcatga tttctgcaat acctgctttg tcatcggcac
    10201 ctaacaaggt tttaccatcg gtggtaatca acgtctgacc cagtagctga tgcagcaccg
    10261 ggaacataac cggtgataaa acttcatcgc cgatacccag cgcaatatcg ccaccgcgat
    10321 agttttcaac aatttgcgga ttaacatttt tgccgctgca atccggtgag gtatccacat
    10381 gagaaataaa gccaatcgcc gggatatcgc cagggacgtt agccggtaac gtcgccatca
    10441 aagtgccctt ctcacttaag gtcacattga taagccccat ttcttcgagc tgctctttca
    10501 gcagatgcaa taacttccat tggccttccg tgctgggaac ctgtctcacc cctgcttttg
    10561 attgggtatc cagagatacg tagttcaaaa atcgctcaag tagtttatcc atgtagtcac
    10621 cctcactttt tgtgacaaca ttattaagaa gcaagaaaag acaaatattg cgtcaggtca
    10681 cttttacccc tgcaagcggg aatatttatc agcattacct taatgaatat aaagctaagc
    10741 cagtaattca caacaaagat tggcgattca agcggtttgc cgtagaatta ccgccctcat
    10801 taagagtcac caaggtggtt aaccacaaac cccgcatcgg taagccatcc gttgcgttta
    10861 catgggacag agtaaaaaat tgaataaaca accgagttcg ctttcaccgc tggtgcaatt
    10921 ggcgggaatt cgcaaatgct ttgatggtaa agaggtcatt ccccagctgg atctgactat
    10981 caacaatggc gagttcctca cgctgcttgg cccttctggc tgcggtaaaa caaccgttct
    11041 tcgcctgatt gcaggtctgg aaactgttga ttccggacgc atcatgctgg ataacgagga
    11101 catcacccac gttccggcgg aaaaccgcta tgtgaacact gttttccaaa gctacgcact
    11161 tttcccccac atgaccgtgt tcgaaaatgt ggcctttggg ttgcgcatgc aaaaaacccc
    11221 cgctgctgaa attacgcccc gcgtgatgga agccctgcgg atggtgcaac tggaaacctt
    11281 cgctcaacgt aaaccgcatc agctctctgg tggtcaacag caacgcgtcg ccattgctcg
    11341 cgcggtggtt aacaagcctc gtctgttgtt gctggatgag tcgctctcag cgctggatta
    11401 caaactgcgt aagcaaatgc agaacgagct gaaagcgtta cagcgtaagc ttggtattac
    11461 cttcgtcttt gtcactcacg atcaggaaga agcgctcacc atgtcagaca ggattgtggt
    11521 gatgcgcgag ggtcgtattg agcaagatgg tacaccgcgc gaaatttacg aagagccgaa
    11581 aaacctgttt gttgccggct tcattggcga aatcaatatg tttaacgcca ctgtcatcga
    11641 acgtctcgac gagcagcgcg tacgcgccaa cgttgaaggc cgcgaatgta atatctacgt
    11701 taacttcgcc gttgaaccgg ggcaaaaact gcatgttctg ctgcgcccgg aagacttacg
    11761 tgttgaagag attaacgacg acaaccacgc tgaagggctg attggttacg ttcgtgagcg
    11821 taactacaaa ggcatgacgc tggagtcggt tgttgaactg gaaaatggca agatggtgat
    11881 ggtcagcgaa ttcttcaatg aagacgaccc tgactttgac cactctctcg accaaaaaat
    11941 ggccattaat tgggtagaaa gctgggaggt cgtactggct gatgaagaac acaagtaagt
    12001 tccagaatgt agtgattgtc actattgtcg gttggcttgt gttgtttgtc tttctgccca
    12061 acctgatgat cattggcacc agctttttga cccgcgacga cgccagtttc gtcaaaatgg
    12121 tctttacgct ggataactac acgcgtctgc tcgatccgct ctattttgaa gtgctattgc
    12181 actcgctgaa tatggcgctg atcgccaccc tcgcctgcct ggtgctgggc tacccgtttg
    12241 cctggtttct ggcgaagctg ccacacaagg tgcgtccgct gctgctgttt ctgctgattg
    12301 ttcccttctg gaccaactca ctgattcgca tctacgggct gaaaattttc ctcagcacca
    12361 aaggctatct caacgagttt ttgctctggc tgggcgttat cgacacacca attcgtatca
    12421 tgttcacgcc cagtgcggtg attatcggtc tggtttacat tctgctaccg tttatggtga
    12481 tgccgctgta ctccagtatc gaaaaactgg ataagccgtt gcttgaggcg gcgcgcgatc
    12541 tcggtgccag caagttacag acttttatcc gtatcattat tccactgacg atgccgggaa
    12601 ttattgccgg atgtctgctg gtgatgctgc cagcgatggg cctgttctat gtatccgacc
    12661 tgatgggcgg tgcgaaaaac ctgctgatcg gtaacgtcat caaggtccag ttcctcaata
    12721 ttcgtgactg gccgtttggt gcagccacca gcattacgct gactatcgta atgggcctga
    12781 tgttgctggt ttactggcgc gcttctcgtc tgctgaataa aaaggtggaa ctcgaatgat
    12841 cggtcgactg cttcgcggcg gttttatgac cgctatctac gcgtacctgt atatcccaat
    12901 cattattttg attgtgaact cctttaacag ctcgcgcttt ggcatcaact ggcagggttt
    12961 taccaccaaa tggtatagcc tgctgatgaa caacgacagc ctgttacagg cagcgcagca
    13021 ttcactaaca atggcggtgt tttcggcgac atttgctacg cttatcggtt cactgacggc
    13081 agttgcgctg taccgttatc gctttcgtgg taagccgttc gttagcggaa tgctgtttgt
    13141 ggtgatgatg tcaccagata tcgtgatggc gatttctctg ctggtgctgt ttatgctgct
    13201 gggtattcag cttggcttct ggtcgctgct gttctcgcat atcaccttct gcctgccgtt
    13261 tgtggtggtg acggtgtatt cgcgcctgaa aggttttgac gtgcggatgc tggaagcggc
    13321 gaaagatctc ggtgccagtg aatttaccat tctgcgaaaa atcattctgc cactggcaat
    13381 gccagcggtg gcggcgggct gggtgttaag ctttaccctg tcgatggacg atgtggtagt
    13441 ttcttcgttc gtcaccggac caagttatga aattctgccg ttaaaaattt attcgatggt
    13501 caaagtcggc gtatcgccgg aagttaacgc gctggcaacc atattactgg tgctgtcgct
    13561 ggtgatggtt attgccagcc agcttattgc tcgtgataaa acgaaaggta acacagggga
    13621 cgttaaatga aaaaatggtc acgccacctg ctcgcggcgg gtgctctggc actgggcatg
    13681 agcgccgctc acgccgatga caacaacacg ctgtatttct acaactggac cgagtacgtg
    13741 ccgccaggac tgcttgaaca gttcaccaaa gaaaccggta ttaaggctat ctattcgact
    13801 tacgagtcga acgaaaccat gtacgccaag ctgaaaacgt acaaagacgg tgcctatgac
    13861 ctggtggttc cttcaaccta ttacgtcgat aaaatgcgta aagaagggat gatccagaag
    13921 atcgacaagt cgaagttaac gaacttcagc aatctcgatc cagacatgct caacaagccg
    13981 tttgacccga ataacgacta ctccattccg tatatctggg gtgcgacggc gattggcgtt
    14041 aacggtgatg cggtggatcc taaatctgtc accagttggg cagatctgtg gaagccagag
    14101 tacaaaggta gcctgctgct gaccgacgat gcccgtgaag tgttccagat ggcgctgcgc
    14161 aaactgggct actccggtaa caccaccgat ccgaaagaga ttgaagctgc atataacgag
    14221 ctgaaaaaac tgatgccaaa cgtcgcagcg tttaactccg ataacccggc taacccgtac
    14281 atggaaggcg aagttaacct cggcatgatc tggaacggct ctgctttcgt tgcacgccag
    14341 gcgggtacgc caattgacgt ggtgtggccg aaagaaggcg gcattttctg gatggacagc
    14401 ctggcgatcc cggcaaatgc caaaaacaaa gaaggcgcgc tgaaattgat caacttcctg
    14461 ctgcgcccgg atgtggcaaa acaggttgcc gaaactatcg gttatccaac gccaaacctt
    14521 gcggcgcgta agctgttaag cccggaagtg gcgaacgata aaacgctcta cccggatgct
    14581 gaaaccatta aaaatggtga atggcagaat gacgttggcg cagccagcag catttatgaa
    14641 gagtattatc agaagctgaa agcaggacgt taattcagca aacgggcggt ggtattaccg
    14701 cccgtttttt agttagcaag gatgtggcta atgagtcagg acagtaaagt tttatcccgc
    14761 tctttcgtcg gtattgtggt ccccttgtag gcctgataag cgtagcgcat caggcaatgc
    14821 ttcctgcttt ataatccctt cagcaacttt tcaacaaact caggcaccac ctggcttgcc
    14881 gggccgtaat atttctcggc aaattcatta ccgacctgac ttggttcgag attcagttcc
    14941 acggtgtgcg cgccatgcag tttcgcttcg tgaacaaacc cagccgccgg ataaacatgc
    15001 ccggaagtac caatggcaat gaaaatatca gccatcgaca acgccatata aatttcatcc
    15061 atgccgagtg gcatttcgcc aaaccaaacg acgtgcgggc gcaagggtgc cgggaactgg
    15121 caacaatggc atttatcttc tggcgtaacg tctccggtcc agtcgagaac ctgaccactt
    15181 tgcgaacaac gaactttcag caattcccca tgcatatgaa tgacattggt attacctgcg
    15241 cgttcatgca ggttgtctat attctgcgtt accagcaaaa agcgatcgcc gagggcgtct
    15301 tgcagtttag ccagcgcaag atgcgcggcg ttaggctgaa tttctggctg ctgcagctgt
    15361 cgacgacggg cgttataaaa cgcttgcacc agttcaggat cgcgatcgaa accttccggc
    15421 gttgccacat cttcaacccg atgttcttcc cacaggccat ctgcggcgcg aaaggtacga
    15481 atacctgatt ccgcagaaat tcctgcccct gtcagtacga gtactcttgg tttttccatt
    15541 gcttccggca ccactttatc tctgaaaaaa atacgctgac gcaaacgctc gcgcaggcgg
    15601 cgtttatttt tacgaaaacg acttaaccga tgaccccgac gcgacagcat agcaacctct
    15661 gttgtttaat cggttagatg taggaaggcc gcaccacgca ttcctcccgc atcaccgtgg
    15721 cgcgcgcgtt caatgcgcgg aacacgagct acaggtaaga gatgacgagg cagcctgtcc
    15781 gccagttgcg ttgtgattgc cgggaaattc gataagccac caccaatgac gaccaggtca
    15841 gagtcaacga tggtcaggat atttcccaga caaaccgcta ataaatccag ataacgctca
    15901 acgtgcgccc ttgcctgctc atcgccttga tcataaagcg caataatttc gggagcctgc
    15961 aacggttgat gataatagtg ttgccagagc cacgcaaagc cccgacctga tagataattt
    16021 tcgatacaac caatttgacc acagccgcag cggcgtaacg ggaaatccag ccccatcatg
    16081 gttaacgcat caaccggcag acgcatatgg ccaaactcgc cggtaatgta gcttttaccg
    16141 gtaatcggtt taccgttgaa aatcagcccg ccgccaacgc cggtgccgag aattaacccc
    16201 atcaccagtg gatattgcgt aaattcatca tcccaggctt ctgaaagggc aaaacagttg
    16261 gcatcgttat caaggcgtac atcgcgatca agacgtgcgc tcaggtcggc acgcagcggt
    16321 ttaccgctgg cagcggggac attggcggca tacagcgtac catcttctgt ttccggcatt
    16381 cccggaatac cgatgccaac agaaccttta cagccaaaac gttggtcagc ttcggctacc
    16441 agctcgcata ctgcatctaa aaatgcgtca tagctgtcac gcggtgtcgg cacccgcttt
    16501 tcccactgca actgccgacc gctatcaaac acgccaagcg caatttttgt tccaccaata
    16561 tcaaacccgt aatacattgc cgctccttgt tttaatgtac tgcctttact ggccgctaag
    16621 gactcgcgca gggtcaatat tactggcgcg ccgcgccgga taccaacttg ccaaaagact
    16681 cagcaacaat gctgtgacca gtacgtagaa gacgtccagc cagtgcaatt ccgatggcag
    16741 gaagtcaata aaatagatat cgctggagag gaactgatga ccgatcagct tttcaatccg
    16801 ctcaataatc ggggtaagtt gcagtgaaac aactacgccg ataatcacac cacacaggct
    16861 accgaacagc cctgccagca atccatacca gacaaagatg gcgcgaatta aaccatcttt
    16921 cgcccccagc gttcttaata ctgcgatatc gccactcttg tctttcaccg ccatcactaa
    16981 ggtggagacg atgttgaaac aggccacgcc aatcaccagt accatcgcca gatacataat
    17041 ggcgcggatc atctggatat cgcgatacat atagccgtaa gtaccaatcc aacttttaat
    17101 ataaacatag ctgttggtca cttcacccgc atcgcgtacc agcttattgg cgttgaaaac
    17161 atccgtcatt ttaagggcaa tacctgacac gctggaaccc atatcaagat attgttgggc
    17221 atccgccagc gggatcatgg caaaactgtg atcgagttga ccactcaact gcaaaatacc
    17281 ggcaatgtgc aaacgcacac gttttggctg catcagttta tgctcgggat tcgagttggg
    17341 gatcatgatc gacacccaat cgccctgctt cactttcagc gcatccgcca cgcctttgcc
    17401 gatgataatt tgctgttcgc ccgctttaaa attgcgccag gcatccccct gaacaaacga
    17461 gggtaatgcg ctcagacgct gttcctgttg cgggttaacg cccttcacct gaattgcgcg
    17521 cagattggta ccactttcta ccagtccggt gaaattgata tacggcgcag cggcggcaat
    17581 acccggcact ttttgcacgt tatccagtgc ttcctgccag ttagtccatg gctgatttac
    17641 cgcctcaatt tcaccgtgcg gtaccacagc aagaatgcgg ttattcagtt cgcgttcaaa
    17701 gccgttcatc gcgcttaagc cgacgatcaa taccgccacg ccgagggcaa tgccaatggt
    17761 agaaatgaca gagattagcg acaccatgcc gccgcgtcgc cgtccgcgac taaaacgcag
    17821 gccaatcaat aacgataaag gcatcgccat tactccgccc ccatcaggct cagttccgcc
    17881 gtcagacgtc catcacgcat ttccagttgg cggctcatac gtttcgccag ttgcaggtcg
    17941 tgagtaacca ccaggaaggc ggtgccctgc aagcgattca attccccaag caactggaag
    18001 atgctgtcgg cgttacgcgc atcgaggtta ccggtaggct catccgcaag taccagacgc
    18061 ggattattga ccagcgcacg ggcaatcgcc acacgctggc gttcgccgcc agaaagttca
    18121 gatgggcggt gattcgcacg atgctccagc cccaccgctt ttaacatctc cagcgcacgg
    18181 ctgttgattt cagcgggctt tttcttgcca atcagcagtg gcatagccac gttttccagg
    18241 gcagtaaaat ccggcagcag gtggtgaaac tgataaataa agcccagctt ctggttgcgc
    18301 agttctgctt tcgccgccga agacagtttg ctcatcggtt gaccgttaaa gatcacatcg
    18361 ccggaggttg gtgtatccag cccgcccagc aggtgcagca aggtactttt accggaacca
    18421 gagctaccga cgatcgccat catttcacct tcgccgacgc tgaaactgac attgtgcagt
    18481 acatcggttt gcacactgcc ttcctgatag cgtttgcaca ggttgtcgca ttgcaacagg
    18541 atcttattca taacgtaaag cctcagcggg ttgagtggcg gcagcgcgcc atgaagggta
    18601 aagcgtagac agcagcgcga tagccatcgc caccagcgca ataacaatga cctgtaaagg
    18661 ttcgatagcg accggcagcg ccgcgccatc aagcaggacg ccgattatcg gcatcagatt
    18721 atttaactgg ctggcaagca gtgcgccaag cgccgctccg aggatcgcac cgataatccc
    18781 ggcgctggcc ccttgcacca taaagaccat catgatttgt cgcggagtta agccttgcgt
    18841 ttgcaggatc gcgacttcgc cctgcttctc cattaccatc agccctagtg aggtaataat
    18901 gttaaacgcc gcaacggcga caatcaggct cagcagcaag cccatcatat ttttttccat
    18961 gcgtacggcc tggaacagct cgcctttacg gtcacgccag tcctgccatt tgctgccttc
    19021 aggcagtttt tgctgactta aagagtcaac tttcagcggc tcatccagcc acaaacgcca
    19081 gccggtaata ttgcctgccg gataacgcat cagacgcgag gcatcctcaa tattcaccag
    19141 catttcatag ccatcgactt cactgttggc ggcgaaagtg ccaatcacat tgaacaggcg
    19201 ctggcttgga atacgcccca tcggcgtgaa ctggctggca gatggtacca tcacgcggat
    19261 ttgatcaccg cgattaacgc ctagctgtga ggcaagttgt tcgccgagga tgacattata
    19321 tttccccggc tcgaggtcag tttgtttcac attgaccaga tacggcgtaa gtggatcttt
    19381 ttgcgccggg tcgataccga gcatcacccc gaccgccacg ctgcgcgcgc tttgcagtac
    19441 cacatcaccg gtagtaatag gtgcgacgcg attaacgccg tccagtttga ctgccgtttc
    19501 cgggagttgc tgcgggttaa gagagccatg ctcagaagag agaattgcct gtggcatcag
    19561 gccaaggatg ttgttttgca gctcgcgctc aaagccgttc atcactgaca atactgtgac
    19621 cagcgccatc accccgaggg taatgccgat ggtagaaagc caggagacga aacgaccgaa
    19681 gcgatccgct gcacgcccac gcatgtaacg caggccaata aatagagcga caggttggta
    19741 catgaaatcc gtctggttgc tgtagcaaag acacgagtat ataagcgaaa gcaagatggg
    19801 taaacgtctg aactgtaagt gatcgtcagg gctaatctga aaagcccccg taaatcaatc
    19861 ggctaaattg ccatgtttta tcatcaatct tgccgcctga tcttaatctg aaaaatcatc
    19921 caatacagac agatacccat tacacctcgg gtcataaccc gcaggatcaa ccgcatgtaa
    19981 attaaaaaag agcgtgttgt cctgatgaaa caaaaagagc tatggattaa ccagatcaaa
    20041 gggttatgta tttgtctggt ggtgatttat cactcggtca ttacctttta tccgcatatg
    20101 accacttttc agcatccgtt atcggaagtc ctgagcaaat gctggatcta tttcaatctt
    20161 taccttgcac cctttcgtat gccggttttt ttctttatat ccggctattt gattcgccgc
    20221 tatatcgaca gcgtaccgtg gggaaattgt ctcgataaac ggatctggaa catcttctgg
    20281 gtgctggcgc tttggggcgt ggtgcagtgg ctggcgttaa gtgcgctgaa ccagtggctg
    20341 gcacctgagc gcgatttaag caatgcctcc aatgccgctt atgccgattc caccggtgag
    20401 ttcctgcacg ggatgatcac cgccagcacc agcttgtggt atctgtatgc gttaattgtc
    20461 tatttcgtgg tatgtaaaat ttttagccgt ctggcgctgc cactattcgc cttgtttgta
    20521 ctgctgagtg tggcggttaa tttcgttccc acgccgtggt ggggaatgaa cagtgtgatc
    20581 cgcaatttgc cttattacag ccttggcgca tggtttggcg caacaataat gacctgtgtt
    20641 aaagaggtgc cgttgcgccg ccatctgctg atggcttctt tgcttaccgt tctggcggtc
    20701 ggtgcctggt tgtttactat ctcgctgctg ttgtcgctgg tatcgattgt ggtgatcatg
    20761 aagctgtttt atcagtacga gcaacgattc ggtatgcgct ccaccagcct gctgaatgtg
    20821 attggctcca acaccattgc tatctacacc acccatcgca ttctggttga aatattcagc
    20881 ttaactctgc ttgcgcaaat gaacgcagca cgctggtcgc cgcaagtcga actgacactc
    20941 ctgctggttt atccctttgt cagtttgttc atctgtactg ttgcgggctt gctggtaaga
    21001 aaactttcac agcgtgcatt cagcgatctg ttgttctccc cgccttcact gcccgctgcc
    21061 gtcagttact ctcgttaaac catcgcccca gccggggcga ttcaatttgc taaaccatgt
    21121 cgattacgga taatgaacag cattgcttat cagagatatg cccccatatg ttgaggcata
    21181 tcctaacgag aatctgacaa ccgttatgcc tgaacaatat cgttatacac tgcccgtcaa
    21241 agcgggtgag cagcgtctgc tgggcgagtt aaccggcgca gcctgtgcaa cgctggtagc
    21301 ggaaattgcc gaacgtcacg ccggtccggt ggtactcatt gcaccagata tgcaaaatgc
    21361 tctgcgtttg catgatgaaa tcagccagtt caccgatcag atggtgatga atctggcgga
    21421 ctgggaaact cttccctacg acagtttttc gcctcatcag gacattatct cctcgcgcct
    21481 ttccaccctt taccagctac cgacgatgca gcgtggcgta ctgattgttc cggtgaatac
    21541 gcttatgcag cgcgtttgcc cacacagttt tctccacggt catgcgctgg tgatgaaaaa
    21601 aggtcagcgc ctgtcacgag atgcattacg aacccaactg gacagcgccg gttatcgcca
    21661 tgttgaccag gtgatggagc acggcgaata cgccacgcgc ggcgcgttgc tggatctctt
    21721 cccgatgggg agtgagctgc cttatcgtct tgatttcttt gatgatgaaa tcgacagcct
    21781 gcgggtgttt gacgtcgaca gccagcgcac gctggaggaa gtagaagcga tcaatctgct
    21841 gcccgcgcac gaatttccga ccgataaagc ggcaattgaa ctgttccgca gccagtggcg
    21901 cgataccttc gaagtgaagc gcgatccgga acatatttac cagcaagtga gtaaaggcac
    21961 attacctgcc gggatcgagt actggcagcc attgttcttc agcgaaccac tgccgccgct
    22021 gttcagttat ttccctgcca ataccttgct cgtgaatact ggcgatctgg aaaccagtgc
    22081 cgaacgtttc caggctgaca cgctggcgcg ttttgagaat cgcggcgtcg atccgatgcg
    22141 cccgctgttg ccaccacaat cgctctggct gcgggtggac gagctcttct cagagctgaa
    22201 aaactggccc cgggtgcagc taaaaactga acatttaccg acaaaagccg cgaatgccaa
    22261 tttaggtttc cagaaactgc cagacctggc cgttcaggca caacaaaaag cgccgctgga
    22321 tgcgctgcgt aagttcctcg agactttcga cggtccggtg gtgttctcgg tagaaagtga
    22381 aggtcgccgt gaagcgctgg gtgaactgct cgcacgaatt aaaattgctc cgcaacgcat
    22441 tatgcgtctt gatgaagcca gcgaccgtgg gcgttatctg atgattggcg ctgccgaaca
    22501 tggttttgtc gataaggtgc gtaatctggc gctgatctgc gaaagcgatc tgctcggtga
    22561 acgtgttgcc cgccgtcgtc aggattctcg ccgcaccatc aaccccgata cactgatccg
    22621 taaccttgcg gagctgcata ttggtcagcc ggtggtccat ctggagcacg gtgtcgggcg
    22681 ctatgccgga atgaccacgc tcgaagcggg cggcattact ggcgagtatt tgatgctcac
    22741 ctatgccaac gacgccaaac tgtatgttcc ggtgtcgtca ctgcatctga ttagccgtta
    22801 cgcaggtggc gcggaagaaa acgccccgct gcataaactt ggcggcgatg cgtggtcacg
    22861 cgcgcggcag aaagcggcgg aaaaagtgcg tgatgtggcg gcggaattgc tggatatcta
    22921 cgcgcaacgc gccgccaaag agggcttcgc gtttaaacac gatcgtgagc agtatcagtt
    22981 gttctgcgac agcttcccgt ttgaaaccac gccggatcag gcgcaggcca ttaatgcggt
    23041 acttagcgac atgtgtcagc cgctggcaat ggatcgtctg gtgtgcggcg atgttggctt
    23101 tggtaaaaca gaagtggcga tgcgcgccgc tttcctggca gtagataacc acaagcaggt
    23161 ggcggtgttg gtgcctacca cccttctcgc gcagcagcat tacgacaact tccgcgaccg
    23221 tttcgccaac tggccggtac gtatcgaaat gatctcccgt ttccgcagcg ccaaagagca
    23281 gacgcaaatc cttgcggaag tggcggaagg gaaaatcgat attctgatcg gtacgcacaa
    23341 actgctgcaa agtgacgtca agtttaaaga tttaggcctg ctgattgtcg atgaagaaca
    23401 ccgcttcggg gtgcgtcata aagagcgcat taaagcgatg cgcgcgaacg tggatattct
    23461 gacgcttact gcaacgccga tcccacgcac gctgaatatg gcaatgagcg gaatgcgtga
    23521 cctgtcgatt atcgccacgc cgcccgcccg tcgtctggca gttaaaacct ttgtccgtga
    23581 gtatgacagc ctggtggtcc gggaggcgat cctgcgtgaa attttgcgcg gggggcaggt
    23641 ttattatctc tacaatgatg tggaaaacat ccagaaagct gccgaacggc tggcagaact
    23701 ggtgccagaa gcgcggatcg ccatcggtca cgggcagatg cgcgagcgcg aactggaacg
    23761 ggtgatgaat gatttccatc atcaacgttt caacgtgctg gtttgtacaa ccattatcga
    23821 aaccgggatc gacatcccga cagccaacac cattatcatt gaacgcgcgg atcacttcgg
    23881 tctggcgcag ctgcaccagt tacgcggtcg cgtcggacgt tcgcatcatc aggcatatgc
    23941 atggttgctg acaccgcatc caaaagcgat gactaccgat gcacaaaaac gtcttgaagc
    24001 gattgcctcg ctggaagatc tcggggcagg ttttgcgctg gcaacgcacg atctggagat
    24061 tcgcggcgcg ggtgaactgc ttggcgaaga acaaagcggc tcaatggaaa ccatcggttt
    24121 ctcgctgtat atggagttgc tggaaaacgc cgtcgatgca ctgaaagccg gacgcgagcc
    24181 gtcgctggaa gatctcacca gccagcaaac agaagtcgag ctgcggatgc cgtcgctatt
    24241 gccagatgat ttcattcctg acgtgaatac gcgtttgtcg ttctataaac gtattgccag
    24301 cgccaaaacg gaaaacgaac tggaagagat caaagtcgag cttatcgatc gcttcggcct
    24361 gctgccagat ccggcgcgta ccctgctgga tattgcccgt ctgcgccagc aagcgcagaa
    24421 actggggatc aggaagctgg aaggtaatga gaaaggcggg gtgatcgaat ttgccgagaa
    24481 gaatcacgtt aatccggcct ggttgattgg tttgctgcaa aaacagccgc agcattaccg
    24541 ccttgatggt ccgacgcgcc tgaaatttat tcaggatttg agtgagcgga aaacgcgtat
    24601 cgaatgggta cgccagttta tgcgtgaact ggaagagaac gcgatcgctt aatggtaaat
    24661 cgggggcgtt tctgcgcccc catctgcaac atttacaaat tctttgcact tccctgcact
    24721 atccgacact gtcaccatcc ataattcagg cttatctgtt tattacaata accttatatt
    24781 tattattgat ttttggtgat gatcaaaacg cgtttttctc gctggctaac gttttttacg
    24841 ttcgccgctg ccgtggcgct accggcaaaa gccaacacct agccgctgcc gccagcgagc
    24901 agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa atgacggtgg ttctctggaa
    24961 gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt tacaggctaa ccccggcgtt
    25021 gatccttacg taccgcgcgc gggcagcgtg ttaacgatcc cgttgcaaac cctacttcca
    25081 gacgcgccgc gcgaaggcat tgtgatcaac attgcggagc tgcgtctcta ttactacccg
    25141 ccgggtaaaa attcggtaac cgtgtatcca ataggtattg gtcagttagg tggtgacacg
    25201 ctgacaccga caatggtgac caccgtttca gacaaacgtg caaacccaac ctggacgcca
    25261 acggcaaaca tccgcgcccg ttataaagca cagggaattg agttgcctac ggtagtgccg
    25321 gctggaccgg ataacccaat gggccatcat gcgattcgtc tggcggccta tggcggcgtt
    25381 tatttgcttc acggtacgaa cgccgatttc ggcattggca tgcgggtaag ttctggctgt
    25441 attcgtctgc gggatgacga tatcaaaaca ctctttagcc aggtcacccc aggcaccaaa
    25501 gtgaatatca tcaacactcc gataaaagtc tctgccgaac caaacggtgc gcgtctggtt
    25561 gaagtacatc agccgctgtc agagaagatt gatgacgatc cgcagctgct gccaattacg
    25621 ctgaatagcg caatgcaatc atttaaagat gcagcacaaa ctgacgctga agtgatgcaa
    25681 catgtgatgg atgtccgttc cgggatgccg gtggatgtcc gccgtcatca agtgagccca
    25741 caaactctgt aaatcctgga taaaaaaaat ccccgcgaca cggcatgtcg cggggaaaaa
    25801 atcagcagta gcaggcatta atgagggtta atgcttattt ataaattact gctgttccat
    25861 ggagggtatt cggaccggtt acagaagtaa tacggaaaga ttttgcgccc atctcatccg
    25921 ctttttgcgc cagctgctct tccagcgatc ccagatttgt ccccgcgtta gcactgatag
    25981 taccgacttt ttgttggcct tctggcgttg actgaacttc gacagccgca aagttggcaa
    26041 atgacatgga gcttaaaatc gccgcagcga tgagggtttt tacgtttttc ataatagtgg
    26101 ccttatgcag atgaatgacg tcgcaagaaa ttacttaacg atcgataact aaatgataga
    26161 t
//
LOCUS       DAZRUF010000032        25637 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZRUF010000032 DAZRUF010000000
VERSION     DAZRUF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.032"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(64..795)
                     /gene="rlmB"
                     /locus_tag="ACHXOL_001067"
     CDS             complement(64..795)
                     /gene="rlmB"
                     /locus_tag="ACHXOL_001067"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR6808110.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(975..3416)
                     /gene="rnr"
                     /locus_tag="ACHXOL_001068"
     CDS             complement(975..3416)
                     /gene="rnr"
                     /locus_tag="ACHXOL_001068"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR6808111.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3455..3880)
                     /gene="nsrR"
                     /locus_tag="ACHXOL_001069"
     CDS             complement(3455..3880)
                     /gene="nsrR"
                     /locus_tag="ACHXOL_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR6808112.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4085..5383)
                     /gene="purA"
                     /locus_tag="ACHXOL_001070"
     CDS             complement(4085..5383)
                     /gene="purA"
                     /locus_tag="ACHXOL_001070"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR6808113.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5487..5684)
                     /locus_tag="ACHXOL_001071"
     CDS             complement(5487..5684)
                     /locus_tag="ACHXOL_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR6808114.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5766..6770)
                     /gene="hflC"
                     /locus_tag="ACHXOL_001072"
     CDS             complement(5766..6770)
                     /gene="hflC"
                     /locus_tag="ACHXOL_001072"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR6808115.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6773..8032)
                     /gene="hflK"
                     /locus_tag="ACHXOL_001073"
     CDS             complement(6773..8032)
                     /gene="hflK"
                     /locus_tag="ACHXOL_001073"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR6808116.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8118..9398)
                     /gene="hflX"
                     /locus_tag="ACHXOL_001074"
     CDS             complement(8118..9398)
                     /gene="hflX"
                     /locus_tag="ACHXOL_001074"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR6808117.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9475..9783)
                     /gene="hfq"
                     /locus_tag="ACHXOL_001075"
     CDS             complement(9475..9783)
                     /gene="hfq"
                     /locus_tag="ACHXOL_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR6808118.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9869..10819)
                     /gene="miaA"
                     /locus_tag="ACHXOL_001076"
     CDS             complement(9869..10819)
                     /gene="miaA"
                     /locus_tag="ACHXOL_001076"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR6808119.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10812..12659)
                     /gene="mutL"
                     /locus_tag="ACHXOL_001077"
     CDS             complement(10812..12659)
                     /gene="mutL"
                     /locus_tag="ACHXOL_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR6808120.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12669..14006)
                     /gene="amiB"
                     /locus_tag="ACHXOL_001078"
     CDS             complement(12669..14006)
                     /gene="amiB"
                     /locus_tag="ACHXOL_001078"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR6808121.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14025..14486)
                     /gene="tsaE"
                     /locus_tag="ACHXOL_001079"
     CDS             complement(14025..14486)
                     /gene="tsaE"
                     /locus_tag="ACHXOL_001079"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR6808122.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14458..16005)
                     /gene="nnr"
                     /locus_tag="ACHXOL_001080"
     CDS             complement(14458..16005)
                     /gene="nnr"
                     /locus_tag="ACHXOL_001080"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR6808123.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16004..17143
                     /gene="queG"
                     /locus_tag="ACHXOL_001081"
     CDS             16004..17143
                     /gene="queG"
                     /locus_tag="ACHXOL_001081"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR6808124.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17126..17179)
                     /locus_tag="ACHXOL_001082"
     CDS             complement(17126..17179)
                     /locus_tag="ACHXOL_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808125.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17413..17488)
                     /locus_tag="ACHXOL_001083"
     tRNA            complement(17413..17488)
                     /locus_tag="ACHXOL_001083"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17453..17455),aa:Gly,seq:gcc)
     gene            complement(17524..17599)
                     /locus_tag="ACHXOL_001084"
     tRNA            complement(17524..17599)
                     /locus_tag="ACHXOL_001084"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17564..17566),aa:Gly,seq:gcc)
     gene            complement(17757..17832)
                     /locus_tag="ACHXOL_001085"
     tRNA            complement(17757..17832)
                     /locus_tag="ACHXOL_001085"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17797..17799),aa:Gly,seq:gcc)
     gene            complement(18043..18588)
                     /gene="orn"
                     /locus_tag="ACHXOL_001086"
     CDS             complement(18043..18588)
                     /gene="orn"
                     /locus_tag="ACHXOL_001086"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR6808126.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18683..19735
                     /gene="rsgA"
                     /locus_tag="ACHXOL_001087"
     CDS             18683..19735
                     /gene="rsgA"
                     /locus_tag="ACHXOL_001087"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR6808127.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19832..20800
                     /gene="asd"
                     /locus_tag="ACHXOL_001088"
     CDS             19832..20800
                     /gene="asd"
                     /locus_tag="ACHXOL_001088"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR6808128.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20822..24145
                     /gene="mscM"
                     /locus_tag="ACHXOL_001089"
     CDS             20822..24145
                     /gene="mscM"
                     /locus_tag="ACHXOL_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR6808129.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24296..>25637)
                     /gene="yjeM"
                     /locus_tag="ACHXOL_001090"
     CDS             complement(24296..>25637)
                     /gene="yjeM"
                     /locus_tag="ACHXOL_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR6808130.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5928 a         7193 c         6502 g         6014 t
ORIGIN      
        1 atcttaccag ctatagacaa aaaaaccatc caaatctgga tggcttttca taattcagac
       61 gaactagctg cgctgacgaa ccgcttcaaa taagcaaatt ccggttgcaa ccgaaacgtt
      121 cagggaagaa acacttcctg ccattgggat gctgatcaac tcatcgcaat gttcacgggt
      181 caggcgacgc ataccttcac cttccgcgcc catcaccagc gccaagcgtc cggtcatttt
      241 gctctgatag agtgtatgat ccgcctcgcc tgccgtaccg acgatccaga tattctcttc
      301 ctgcaacata cgcatggtgc gcgcaaggtt agtcacccga atcagtggaa cgctttctgc
      361 cgcgccgcag gctacttttt tcgccgtagc gttgagctgt gcggagcgat ctttcggcac
      421 aatcaccgcg tgaacgccag cagcgtccgc gctacgcagg cacgcgccga ggttgtgcgg
      481 atcggttaca ccgtcgagga tcagcaggaa cggttgatcg agcgaagcga tcagatccgg
      541 cagatcgttt tcctgatact gacgtcctgg cttcacgcgg gcgataatgc cctgatgcac
      601 ggcaccgtcg cttttctcgt cgagatattg gcggtttgcc aactggataa ccacgccctg
      661 agactcaagg gcgtgaatca gcggtaacag acgtttatct tcacggcctt ttaaaataaa
      721 gacttcctga aaacgttcag gggcgcgctc cagcagggcc tgcactgcgt ggatgccgta
      781 aatcatttcg ctcattaatg tactcgttgt ttacgttttg cctgatgcac tacgtttatc
      841 cggcctacat gatctctgca atatattgaa tttgcgtgat tatgtaggcc ggataaggcg
      901 ttcacgccgc atccggcatg aacaaagcgc attttgtcag caatctaacc ctcttctttt
      961 aaagagggta ttgatcactc tgccactttt ttcttcgccg cacgcttcgc tttggtcgct
     1021 gcggctattt tctgcgtttt cgccgatggc tttttcgctt ttctcgcgtc tttcttcgcc
     1081 gctttcggct tcgttttttt ctcaccgcgg aaggcgctgt ctggctcaaa gtttaccttt
     1141 ttaccgacct gacgacgctt gccgcctttt ttgcctgcat cgcctttttt cgctttctcg
     1201 cgcgccgttt taccgacgtt gcgcggtgcg cgttcactgg agatcagact aaagtcgatt
     1261 ttgcgctcgt ccatattaac cgcttcgacg cgaacttcca cgcgatcgcc caggcgataa
     1321 gtctggccgc tggattcccc catcaggcgt tgccctacct ggtcaaagcg atagtagtca
     1381 ttgtccagcg aagagacatg gaccagacca tcaatgaaca agtcgtccag acggacgaag
     1441 aagccaaagc cagtgacgct ggaaattacg cctttaaaga cgttacctac ctggtcgagc
     1501 atgaagtcac acttcagcca gtcagcgaca tcgcgcgttg cttcgtcggc acgacgttcc
     1561 gccatcgaac agtgctgacc cagttgcaac atctcttcca tcgaataatg gtagccgccg
     1621 gtttcagtgg tgttgccctg atgcccctgc tctttcgcca gcagatactt aatggcgcgg
     1681 tgcagcgtca ggtcaggata acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat
     1741 gccaggccaa agtgaccacg gttttctgga tcgtaaatcg cctgtttcat cgagcgcagc
     1801 agcatggttt gcagcatttc tgcatcagga cggtcggcaa ccgactccag cagttccgcg
     1861 tagtcacgcg gttccggctt gttaccaccc ggcagctcca gccccagctc cgccagcact
     1921 gaacggaaag aggtaatcgc ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt
     1981 tctttcgctt tctcaacgaa acgcgccgcc gagatattcg ccagaatcat gcactcttca
     2041 attaatttgt gcgcgtcgtt acgctgggtc tgttcgatac gttcaatacg gcgttcagcg
     2101 ttgaaaatga acttcgcttc ttcgctctca aatgagatcc caccgcgttc ttcacgggct
     2161 ttatccagca ctttatagag gttatgcaac tcttcgagat gcttaaccag cggggcgtac
     2221 tgctcgcgca gatcctgatc gccctgcaga atatgccaga ctttggtgta ggtcagacgc
     2281 gcgtgagagc tcatcaccgc ttcgtagaat ttgtagcccg tcaggcggcc tttcgacgaa
     2341 accgtcatct cgcacaccat acacaggcgg tctacctgcg ggttgagcga acacaggccg
     2401 ttagagagca cttccggcag catcgggata acctgcgaag ggaagtacac cgacgtgcca
     2461 cggttacgcg cttctctgtc cagcggcgtt ggcggacgca catagtagct gacgtcggca
     2521 atcgcgaccc ataaacgcca gccgccgccg cgttttttct cgcagtaaac tgcatcgtca
     2581 aagtcacggg cgtcttcgcc atcaatggtg accagcggta aatcgcgcaa atcgacacgg
     2641 cccgcttttg cttcttccgg cacttcttct ttcagccccg caacctgttg ctcaacagcc
     2701 tgcggccaga tatacggaat ttcatgggta cgcagagcga tatcaaccgc catgccggtg
     2761 cccatattgt cgcccagcac ttcgacgatt ttacccaccg ctttggtgcg gcgagtcgga
     2821 cgctgagtca gttcgactac gaccacaaag cccatccgcg cgcccatgat ctgatcgggc
     2881 gggattaaga tatcgaagct cagacggcta tcgtcaggaa ccacaaagcc gacgcccgct
     2941 tcggtaaagt agcgaccaac aatctggctg gtttttggca ccagtacgcg gacaatacgc
     3001 gcttcacgac gacctttacg gtcagcaccc agcggctgcg ccagcacctg atcgccatga
     3061 atacaggttt tcatctgctc gctggagaga tacaaatcat ctttacgccc ttcaacccgc
     3121 agaaagccgt agccatcacg gtggccaata acggtacctt tcaccaggtc gaggcgttcc
     3181 ggcagcgcat agcactgacg acgagtgaag accagttgac catcgcgctc catcgcgcgc
     3241 aggcggcgac gcaggccttc aagctgctct tcgccttcaa tgtgcagttc taccgccagc
     3301 tcatcacggc tggccggttt ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta
     3361 gggatgggat tcgcgtattt ttcagcttcg cgttcctgga aaggatcttg tgacatctcg
     3421 gttcctccgt tgtcatctct gatgaagatt ttcgtcactc caccagcaat aatttataaa
     3481 gcggttgatt ctcttcaacc aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac
     3541 tttgcacggc cttagaaagc gcctgtttca accgacaggc aggtgtaatg tggcaaaact
     3601 cactgctgca attcaccagc gataagggct ccagctcgcg caccacatca ccaatacgta
     3661 tcgcactcgc cggtttaccc aggcgaatgc cgccattttt tccacgaaca gcagtcacgt
     3721 agccggcacg actaagttga ttgattattt tgaccatatg attacgggag acgccgtaga
     3781 cgtcagtcac ttcagaaata ctggtcatcc gcccttctgg caatgacgcc atgtagatca
     3841 gcgcacgtaa tccgtaatca gtgaaactcg ttaactgcac atcaacctca aaagggaaat
     3901 cgggaaaaaa tacatttata ttgatgataa accagccaca agctgtgtcg ctaatttatt
     3961 tcagttcggg gaaggaaaaa agcgaggatt taacactgtg tcggatagcg taatggctta
     4021 accgacacag caatgtatca cactgttagc ccggcaggca aaatatctgc caggcgtacc
     4081 agaattacgc gtcgaacgga tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag
     4141 agatgatatc gatcggcaca ccggtcagct cttcaatacg cttgatgtag ttcagcgccg
     4201 cctgcggcag gccgctacga tctttcacgc cgaaagtgga ttcagaccag cccggcatgg
     4261 tttcgtaaat cggctctaca cctttccagt cgtcagctgc cagcggagtg gtagccactt
     4321 cgcgaccatc cggcatacgg taagccacgc agagtttaac ctctttcagg ccatccagaa
     4381 cgtccagctt ggtcaggcag aagccagaca gggagttcag ctgtaccgca cgacgaacgg
     4441 caacggtgtc cagccagccg gtacgacgac gacgacccgt agttgcgccg aattcgttac
     4501 cctgcttgca gaggaactcg ccagtttcat caaacagttc ggtcgggaac ggacctgcac
     4561 ctacacgagt tgagtaagct ttgaggatac ccagaacata atcaacataa cgcgggccca
     4621 ggccggaacc ggtcgccacg ccaccagcag tggtgttgga agaagttacg tacggataag
     4681 taccgtggtc gatatccagc agcgtaccct gcgcaccttc aaacatgacg aaatcgccac
     4741 gctgacgcgc ctggtcgagc aggtcagaaa cgtcaaccac catagaagtc aggatgtcgg
     4801 caacagccat cgtatcatcc agaactttct ggtaatcaac cgcttcagct ttgtagtagt
     4861 taaccaactg gaagttgtga tattccatca cttctttcag tttttcagcg aaggtttctt
     4921 tgtcgaaaag gtcgccaaca cgcagaccgc gacgtgctac tttatcttca taagcaggcc
     4981 cgataccacg accggtggtg ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat
     5041 ccagcgcaac gtgataatca aggatcagcg gacatgcttc agacagcagc agacgctcac
     5101 gaacggggat gccacggtct tccagttctt tcatctcttt catcagcgcg gccggagaca
     5161 gcacaacacc gttaccgatg atgctggtta cattctcgcg gagaatacct gatggaataa
     5221 gatggagaac ggttttttca ccgttgatta cgagagtatg gcctgcgttg tgaccgccct
     5281 ggtagcgtac aacatattta gcccgttcag tcagaagatc gacgatctta cctttacctt
     5341 cgtcacccca ttgggtgccc agtacgacga cgttgttacc catttttcaa aatcaccgtt
     5401 tgcttaaaaa tggattctac catcgctttt tcagagttac agcacttttt gcactcaaaa
     5461 atgacctcga tcagtctgtt ttttgttcag ccaatcgttt tcctcaacat gtagtagacc
     5521 acaacgcccg caaccacaag tccaccgcca aaacgacgta aaatattatc gggcaaattg
     5581 gtcatcgcag agatcatctt cttccatgcc ttcgggtaaa gcatcggccc taaaccttcc
     5641 agtaccaaaa ccagggcaag cgccagccag attgtcgaat tcattcatta tccttataga
     5701 aaaagaaaac caccgacatc cctgaggatg cggtggcttt attgacctgt accgcagtcg
     5761 ttatattaac gcgttgcgga agtcggcgtc ttcatgtagc ggaagaaatc gctatccggg
     5821 ctcatgacca tcacgtcctg attgccagag aagctgttct cataagcacg caggctacgg
     5881 atgaatgcgt agaagtccgg atctttactg aatgcatcag caaacagttt ggctgcttcg
     5941 gcatcgcctt caccacgcat gatgcggccc tgacgctcag cttctgccag cgttctggtc
     6001 acttcatagt cggcagtcgc gcgcagtttt tccgcttctt cctgaccttg tgaacggtga
     6061 cgacgcgcta ccgcttcacg ctcggcgcgc atacggttgt agatcgcttc agacacttcg
     6121 gtcggcaggt tgatctgctt gatacgcaca tcgacaactt caatacccag cgccgccata
     6181 ctgttcgggt tgatgaccgg aactttgccc ttcgtctcag ccgttacgcg ctctgccgct
     6241 tcggcaatgg cgttatctgc cgccggggta gtaacttcat cttctgtacc cgcagaaccg
     6301 gagttcagcg cgtcacgtac ttcgagggtc agacgaccac gggaatcggt gacgatgtct
     6361 ttcacgtcca ggcgaccaat ttcagaacgc agacggtcag agaacttacg tttcaacagc
     6421 acttccgctt gcgaaatgtc gccaccaccc gttgccaggt agtaacggct gaaatcgctg
     6481 atgcgccatt tgatgtaaga atcgacgatc aggtctttct tctctttggt cacaaagcgg
     6541 tcggcctggt tgtccatggt ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac
     6601 ggtatcttga aatgcagacc cggctcataa accagaggtt tgttgtcatc gtcacgcagt
     6661 accttaccaa aacgcagcgt aataccgcgc tcaccttctt tgacgacaaa gacagacatg
     6721 taaagcacta ccagcacgat gatgataatc gcgataactg acttacgcat cgttattccc
     6781 cctgacgctg gtagtcgtta cgctgcgcgt tggcgcggcg ttggtccata atatcgccct
     6841 gactggtgga cgacgtgttg cttgctccac tggttgtgga ggaagaggct ggcggcagac
     6901 gcagcagatt gctggcaccg ttatcgctct tcgccgcagg ggcgttaccg cctttcagca
     6961 tctggtctaa cggcagaacc atcaggttgc cacctttatc gttaaccagc actttgcggg
     7021 tgttacccaa cactttttcc atcgtctcga tatacagacg ctcgcgagta atttccggcg
     7081 cggctttata ttccggcaga agtttagcaa agcgcgccac ttcaccctga gcttccagga
     7141 tggtctgggc cttgtacgca cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac
     7201 gcggctgaac ttcgttggta tacgcttctg cttcacgaat gtattgctgt tcgttttcac
     7261 gcgcggcaat cgcatcgtca aacgccgctt ttacttcttc cggcggacga gctgcctgga
     7321 agttgacgtc cagcagagtg atacccatgt catacggacg gatagtctct tccagttcgc
     7381 gctgagtatc gctacgaatc acggtacgac cttccgtcag aatgcggtcc atggtgtatt
     7441 taccgataac tccacgcagg gcgctgtcgg tagcctgacg caggctgtca tccgggctgg
     7501 tcacgctata cagatatttt tccggattgg tgacgcggta ctgcacgttc atctcaacgc
     7561 gcactacgtt ctcgtccgac gtcagcatca caccagaagc ggccagttca cgcacggctt
     7621 ccacgttcac cggtttgact tcgtcgataa acgtcggttt ccagttcaga cccggctcaa
     7681 ccagatggct gaatttacca aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat
     7741 agaaaccact ggccgcccag ataatgacaa tcgctgccgc tgcgatggta acgacacgac
     7801 cgccaagctg cgggcgcggg ccttgcgatg aactgccacc gccagatccg gtgcctttac
     7861 cgccgcccag accaccgagc tttttgctca gtttgcggaa gatatcatct aaatcaggtg
     7921 gcccttgatc gcgaccgcct ttgtttccat ttccctcaga gttgccgcca ggtttgctgc
     7981 ttccccacgg gtcgcggtct tgtccgttat taccgggctg attccacgcc atgtttgtgc
     8041 tccatatttg ttatgcggtg atccctgttg tctttcgacg ccagaggata tgactccacg
     8101 cttcagacgc tacgccgtta gatcaggtaa tcgatcaacg ccggttcttg tttacagagg
     8161 cgacgccagt caacgatcgg catacgaact tgcagactta cgctgccgtc ctcctccatc
     8221 cactcttttt ctattgcctg aagctgataa aaacgacttc tcagacgtcc ttcctgcggc
     8281 ggcaaacgca atgtatgctg cgccacctcg ccggaaagcc gctccgtcaa agcctgaaaa
     8341 agctgtggta tccccgctcc ggtctgtgcg gaaagccaga cacggatcgg tttgttctct
     8401 tcgtcccgat caatacgcgg ttcgaaatct tccagcatat cgatcttgtt catcaccagc
     8461 agggttggga tctcgtgagc gtcgatctct tcaagaaccg tattcaccgc ttcgatgttt
     8521 tcttgtacac gtacatccgc cgcatcaatg acgtgcagca gtaatgtggc ttgccgcgtc
     8581 tcttgtaacg tggctttaaa tgctgccacc agatcgtgcg gcaggtggcg aataaaccct
     8641 acggtatctg caagtacggt ttcaccgaca tccgcgacgt caatacgccg caacgtcggg
     8701 tcgagggtgg caaacaactg gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa
     8761 agggtagatt taccggcgtt ggtatatccc accagcgaaa cagtaggaac gtcggctttg
     8821 atacgcgatt gccgcccctg ctcacgctgc ttttcaactc tttctaggcg cgactgtatc
     8881 tgcacgatgc gattacgcaa caaacgacgg tcggtttcga gctgggtttc acccggacca
     8941 cgcaaaccta tcccgccttt ctgtctttca aggtgggtcc agccacgcac caggcgcgta
     9001 gccagatggc gcagctgcgc cagctcaacc tgcaacttac cctcatgggt acgcgcacgt
     9061 tgggcgaaaa tatctaaaat aaggccggtg cggtcgataa cacgacactc gcacaaacgc
     9121 tccaggttac gctcttgcgc cgggctcagg gcatggtcaa aaagaacgac cgaagcaccc
     9181 gtcgctttga cagcttccgc aatttcaact gctttacctt cacctacaaa atactttggg
     9241 tgcggcgctt tacggctacc ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc
     9301 agagattcaa actcctggag gtcttccata tctttgtctt gcgtaaaata gatgtgtacc
     9361 agtaccgcct gctcaccagc atcataacgg tcaaacaagc gtataaccct ctaaatagat
     9421 cagcggggaa cgcaggatcg ctggctcccc gtgtaaaaaa aacagcccga aaccttattc
     9481 ggtttcttcg ctgtcctgtt gcgcggaagt attctgcgcg ctgctaccat gatggtagtt
     9541 actgctggta ccgccaccgg cgttgttact gtgatgagaa accgggcgag acgggacaac
     9601 agtagaaatc gcgtgcttgt aaaccatctg gctgaccgtg tttttcaaca ggatcacgaa
     9661 ctgatcaaaa gactcgattt gcccttgcag cttaatacca ttcaccaaat aaatagaaac
     9721 tggaacacgt tcccgacgca gtgcgttcag gaacggatct tgtaaagatt gccccttagc
     9781 cattctctct tttccttata tgcttatttg tactttgaac ctttcgattc tgaaaaaatt
     9841 gcgcacgata cgtctcaatt gtacacattc agcctgcgat agcaccaaca acctgtaata
     9901 cttcgtcacg cgcctgttct ggtttttcac tgtcaagcca gtgaacccct tcccaaccac
     9961 gcagccaggt tatctgccgc ttcgccaact gtctcgtggc gcaaacacct cgataaacca
    10021 tttcatcgta tgagatttcg ccttcaaggt aagaccacat ctggcgataa cccacgcaac
    10081 gaatggaagg caagtccgta tgcaaatctc ctcgggcaaa aagcgcccgg acttctgctt
    10141 caaaacctga agccaacatc tgatgaaaac gctgctcaat gcgttgatgg agcagttcac
    10201 ggctcgccgg ggcgatggcg aactgatgca cctgatacgg tagagcgtct cctgacgttt
    10261 gcgtcagttc cgttaaagtt ttacccgaaa tgaaaaaaac ttccagtgcc cgggaaagcc
    10321 tttgtggatc atttggatga atccttgctg ccgcaaccgg atctacctcc tgaagttgac
    10381 gatgcaatga ctcccaacct tgctctgccg cctgttgctc aattctggcc cgcacttccg
    10441 ggtctgccga cggtagcggc gacaaccctt ccagcaatgc cttgaaatac aacatcgtac
    10501 cgcccactaa cagtgggatc cgccccgccg cggtgatatc ggccatttcc gccagcgcat
    10561 cgcggcgaaa atcagcagcc gagtaagcct gcgacggatc gcgaatatcc agcaatcggt
    10621 gcggcgcggc gagtaactct tcagcgttcg gcttcgccgt cccgatatcc atccctttgt
    10681 aaataagggc agaatcaacg cttatcaact ctactggtaa aattttacgc agctcaatgg
    10741 ctaacgccgt tttaccggag gccgtcggcc ccatcaaaaa aatcgcctta ggcaggctcg
    10801 ccttactgat atcactcatc tttcagggct tttatcgccg gatgtaaatc aacagattgt
    10861 aacagaccac ccggcggcgt tttcacaagt tgcggacata accgttccac gtccgccagc
    10921 agggttatgg cctgtgccat tgaccactgc gcatgttcgc tcatcagatt tcgtgcaatc
    10981 cactgcgcaa tattgccagg ttcgaatacg gactgcttcg ccaggtagcc tatcagttca
    11041 ggaatcaaga tttgtaaatt ttgttggcgt aagggtaaag gcacggccct gatggtcaca
    11101 tgctgtgcat ctgactggaa atcaataccc aattccgcca gggcagactg cgctttttct
    11161 aatgccgatt tttcttcgcc agaaactttt agccgcaacg gaataagcag cggctgagcg
    11221 caaactggcg cttcacccgg cgtcagttgt acctgacgca gccaacgttc tgccactggc
    11281 aaggctaaaa gtgaaatgtt gccgtcgcgc tccagcaacg cacagtcgga atggacgata
    11341 gtcagtaccc gaccaaaact ctgactgttc gccgcaagtg caggttcctg cggttccggc
    11401 gcttttaatt tttgcatcgg cgcgggcgtt tgcaaaagct ggcgatacac ttcaccttgc
    11461 tgtttctggt agcctggctg cgcattcggc cagggggcag ccggacgact gcctgatgct
    11521 ggcgcaggag tgtagcgcgg agctaccggc tcacgagctg ccggttctgc aaagtgattg
    11581 cgccccgccg ccacgcggtt ttccggaatg gaacgcggtg caggttgggg ttcatcgtcc
    11641 agcggtagcg gcgtttccag ttgctgttgt agcacgctca gcacgccctg atagataaaa
    11701 tcatgcacca gacgcgactg atggaaacgc acttcgtgtt tggcggggtg cacgttgacg
    11761 tccacctgat gcggatcgat ctccagatac agcacaaatg ccggttgctg atcggccccc
    11821 agtttgtctt cgcaggcctg gcggatcgcg tgattgatca ggcgatcgcg catcatgcga
    11881 ccgttcacgt agcaatactg aatttctgcc agtgcgggcg tggtgtgatt tggatcggcc
    11941 acccagccgc gtagcgtgag atcgccgtgt tgccattcaa tcgccagcgc ttgttcaaga
    12001 aaagcggtgc cgcaaatcgc gcctaagcgc cgttcttttt gcccgccttc cggcactgcg
    12061 cggtactgac gcacaatttt accgttatgc gacaggttga tcgtgacgtc gaaacgcgcc
    12121 agcgcaatgc ggcggatgat ctcatcaatg tggttaaatt cggttttctc ggtgcgcagg
    12181 aatttgcgcc gcgccggggt gttgtagaac agatccagca cctccagcgt cgtccccaca
    12241 ggatgcgccg ccggttttac cgtcacgttc atatcgcgcc cttcggcata ggcctgccag
    12301 gcttcctgct gttctgcggt gcgtgaagtg agcgtcaggc gggaaaccga actgatactc
    12361 gccagcgcct caccgcgaaa gcccaggctg ataatggctt cgagatcgtc cagagaggcg
    12421 attttactgg tggcatgacg agccagcgcc agcgccagct catctttttt gataccgcag
    12481 ccgttatcac gaatgcggat aagtttcgcc ccaccgcgtt cgatatcaat atcgatacgc
    12541 gtcgcacctg catcgaggct gttttccacc agttctttga ctaccgacgc aggtcgctcg
    12601 accacctcac ctgcggcaat ctggttcgcc agttgtggcg gtaagacctg aattggcatc
    12661 aatcgtcctt agtttggcag cgtgcgatct ggcgtcgtca ccgtactggc agtttgtgcc
    12721 gttgccccct gcggcgcaga ttgcatcgga tgcgcaagga aataattgcg caggcctttg
    12781 taaatggctt ctgccagctg ttgttggtaa tcgtcgctcg ccagcaaacg ttcttcgctg
    12841 ttgttgctga taaaaccggt ttcgaccagt actgacggga tatccggcga acgcagaacg
    12901 ccgagactcg cgtgttctgg tcggcgttta tgtatttcgc caatgcgttg caactgactg
    12961 atcatactgg tcgctacatc ataccctacc cgctgggaat gaccgaactg taaatccagc
    13021 accgcctggc ttaaataggg gtcagactga ctgttcgcca gcacatcgcc cgccccaccc
    13081 agcagctccg actgtttctc gtgctgctcc agccagctgg ccatttcgct gttggcgcga
    13141 cggttagaga gcacccatac ggaagcgcca gtcgcactgc ggttcggtgc ggcatcagcg
    13201 tgaatcgaca cgaggaaatt ggcgttttgc ttacgtgcca catcgctgcg ccccatcacc
    13261 gagataaagt aatccccgtc acgggttaaa acgcctttaa acatcggatc gtcattgagc
    13321 aaagtgcgca atttacgcgc gatggcgatg gtgacatttt tctcccgcgt accaccgggg
    13381 ccgatagcgc cagggtcctg accgccgtgt ccggcatcaa tagcgatgat aattttatcg
    13441 ccagtgttag ccgtcgcgcg cgctgccgga cgcgttaccg tattactgct gataacaccc
    13501 gtagtgcggt tactttccgt tttaaacgga ttgcgcgccg gttcgctgac gcgcggtgcg
    13561 acaaccgcag gcgtttcaac gcgtttcgca accacgggcg gtggaggagg cggtggcggc
    13621 acatcggcgt taatcgtaaa gacgacagtg taattgctgc cattctgccg cttcaccgct
    13681 tcggttttac cgttttcggt aagatcgacc accagccgta gcgtttgtgc atctttaggc
    13741 gttccagagc gaatcgcctt caccagatta ttgccgctga acaacaacgg cagtccctga
    13801 atcacgcccg tttgtttgat atcgagcgcc acggtgcgtt tgctttgatg gctaaacgca
    13861 taatcaggat cgccaataaa actcaacgtt atccgcgcct gttgattacc gttagaaacc
    13921 tgaatatcag agagcgtcgc ggcacccacc ggcgtgcaca gcagcagcag cgtcgctacc
    13981 aaccaatttc tgatgcgata catcatcccg ccacctttca aaggttaacc ggctaaacgc
    14041 gccagcaaca attcacccgc agaggaaacc gcactcacgc gcgcctcacg gccttgtgcc
    14101 tgataatcaa tgtgtatttc gacatccggg tcaggaagaa cacctgtacc ttgttgtggc
    14161 cactccacca ggcagatggc atcgttggca aaataatcgc ggatccccat aaactccagc
    14221 tcctcgggat cggcaaggcg gtacaaatca aagtgataaa ccattaaatt gtcgagcgta
    14281 tagggttcga ccagcgtata agtggggctt ttgacattac cctgatgacc cagagcttgt
    14341 aaaaaacccc ggctaaaggt ggttttacct gcgcctaaat cgccatacag atagattacg
    14401 gttgcgccat cgcaggcttt cgctacccgc tcgcccaggt ctaatgttgc ctgctcatca
    14461 gggagcggaa ttactcgatt catcatggtt tttatcagtc acttccgggt taacaatacg
    14521 ctgtagcgtg gaaaagagat cggttgccag catcccgcgc gttccaaaac gcgccgccag
    14581 tacgtcagct gccgcaccgt gcgcgacaca gcccgcacag gcggcatcat acggcgacag
    14641 tttttgccca agcaatgcgc caataatacc agagagcaca tcgcccatgc cgccgctcgc
    14701 catgcctgca tttccggcat caataatgcc taaagcgtca ggatgggcgg cgaccacggt
    14761 tccggcacct ttcagcaccg ctacgccgcc ataacgttgt accagacgtt tggcgcaatg
    14821 taagcggtca ctttcaattt cagcgacgga acagcctaac aaccgtgcgg cctcgccagg
    14881 atgcggcgtg atcacgcgat tgtgacgctt atcgggattg attgccagca ggttcaatgc
    14941 atcggcatcc cacaacatcg gtttgcgaaa attctcaact ttttgcagtg cttttttccc
    15001 ccactcttgc tggcccagac cgggaccaat caccaccaca tcggcccatt ccaggctttc
    15061 ggtaagagag tccatcgtca gttcatgcac catcaattcc ggtcgtgcag tcagcagcgg
    15121 cgcaatgttt tcactgcggg tcagtactcg gactaaacca gcaccagcac gcagcgccgc
    15181 ttcccccgtc atacgaatag cccccgccgt gccgtgatcg ccaccgataa tcaccagccg
    15241 cccgtgatcg cctttatgcg aagtcgggcg acgcggtttc agccagtgag aaagttgttc
    15301 tgccgaaaac cgctgaattt tcgtctcctg acctgccagc cagctatcca gccccagtga
    15361 gtcaaaatgc agttgtcctg taacatcccg cgcttttcca gtgagcaagc ctggtttcag
    15421 cgcaataaaa gtgatggtgt gatcggcgtt gatcactgcg cctggcgtag cgccggtttc
    15481 agccagcagg ccggaaggga tatcaaccgc cacaatcggc gcaggatggg aattagcgtg
    15541 gtcgattaac tggctaatgg attcgcgggg cgcttgctgc aagccggtac cgagcagcgc
    15601 atcaacaatc agatctaccg attcgggcca gacaatattc gaagcatgga tctcgccacc
    15661 cgcgtttaac catgcttcgc gtgccagcgc ggcctcttcc ggcaacggtt tgtcgctctc
    15721 ctgggccaac aacgtgacct caatgccgac cgctttggcc agtcgcgcga ccacgtagcc
    15781 atcgccgccg ttattaccat gaccgcacag caccagccag tggcgggcgt caggatacgc
    15841 cgaacgacac acctggaatg cggcctcgcc agcgcgaagc atcagctcat agagtgtgag
    15901 ccccagcaca tctgccgcct cgcgttctcc gcggcggata tcgtcggcgt accagacggt
    15961 gtgtggtata cttacggggt ttttcttcat tgtatggtcc gtcatgtcag agcccctcga
    16021 tctcaatcag ttagcgcaaa aaattaaaca gtgggggctg gaactgggct ttcagcaggt
    16081 aggtattacc gataccgatc tcagcgagtc cgagcccaaa ctgcaagcat ggctggacaa
    16141 acaataccac ggcgaaatgg actggatggc acgtcacggt atgctgcgcg ctcgccccca
    16201 tgagttatta ccaggtacgc tgcgtgtaat cagcgtgcgg atgaattacc ttcctgctaa
    16261 cgccgcattt gccagcacgc tgaaaaaccc caaactcggc tatgttagcc gttatgcgct
    16321 gggccgtgac tatcacaaac ttctgcgcaa ccgactcaaa aagctgggcg agatgattca
    16381 gcaacattgt gtttcgctga attttagacc gtttgtcgat tctgcgccta ttctcgagcg
    16441 cccgttagct gaaaaagctg ggctcggctg gacaggtaag cactcactta tcctcaatcg
    16501 cgaggccggt tctttcttct ttttaggcga attgctggtc gatattccgc tgcccgtgga
    16561 tcaaccagtc gaggaaggat gcggtaaatg catagcctgt atgacgattt gcccgaccgg
    16621 tgccatcgtc gagccatata ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga
    16681 acttgaaggg gcgatcccgg aagagttgcg accgttaatg ggaaaccgta tttacggttg
    16741 cgatgactgc cagcttatct gcccgtggaa tcgctattcg caactcacta cagaagacga
    16801 tttcagcccg cgtaagccgc tacacgcaca ggaactcatt gagttatttg cctggagcga
    16861 agagaagttt ttaaaagtca cggaaggatc ggcgattcgc cgtataggtc acctgcgctg
    16921 gctgcgtaat atcgccgtgg cattaggcaa tgcgccctgg gatgaaacga ttctggcggc
    16981 gctggagagt cgcaaaggtg agcacccact tcttgatgag cacatagcgt gggcgattgc
    17041 gcagcaaatc gagagacgaa atgcgtgcat agtcgaagtg caattgccga aaaaacagcg
    17101 tctggttcgg gtgattgaaa aagggttacc gcgtgacgcc tgattcatcc acagcttgtg
    17161 aataaaaata aaaacgcatt gcaattcaag acgcataaaa tcgtcaagtg atcgactcga
    17221 caaaacagaa tcttgatttt taacttattt atcaataggt taaaaaaata ccattcaccg
    17281 tgcgaagttt ctttgaaatt aagattacag cctgaggatg tggataactc tgtttacaag
    17341 agtttgtcag agacaacaaa aaccagcaat aacgcatcgc gtcgctgtgg atattttatt
    17401 gagagaagaa tttggagcgg gaaacgagac tcgaactcgc gaccccgacc ttggcaaggt
    17461 cgtgctctac caactgagct attcccgctt gggtggtctg tgccttacag cactttcaaa
    17521 ttttggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta
    17581 ccaactgagc tattcccgct taatcttcgt ctttcgaacc gctactgcct tggctgcctt
    17641 cactcacctt agtcacatac ttttgtaagt tcccaaggat tcgttcagtt gccgccttgt
    17701 cgcaattcga aatactgcga ttgggtggtc tgtgctttgc agcacttttc aaattttgga
    17761 gcgggaaacg agactcgaac tcgcgacccc gaccttggca aggtcgtgct ctaccaactg
    17821 agctattccc gcgtactact taattttgct ttcgtaattt tcgcatttct gcgtcgttac
    17881 gggaggcgca ttatacgaga aatctttccc tctgcaaccc cccaaaaaag ttttttttgc
    17941 gtttttatct caagtgctga aattatcagc aagacgttca atttagcgtc aaaatcacgc
    18001 cgccgcaagt ggattccggc gcttacgtga ccaggaaaaa ttttacagct tgataaaatg
    18061 ctcgcggtag taagccagct ccgccaccga ttcacggata tcatccattg cctgatgcgt
    18121 cccctgcttg gtaaaaccat ccagaatttc cggcttccag cggcgcgcca gctctttcag
    18181 ggtgctgaca tcgagataac ggtagtggaa gtaggcttcc agctccggca tgtatttaaa
    18241 caggaaacga cggtcctgtc cgatgctgtt accgcaaatc ggcgattttc ccgcaggcac
    18301 ccactgtttt aagaattcga gcgttgccag ttcagcttcc cgatcgccca tcgtgctcgc
    18361 tttcacgcgc tctaccagcc cgctggcggt atgggtgcgc acgttccagt catccatcag
    18421 cgccagctgt tcatcggact gatgtactgc aatggtcggc ccttctgcca gaatattcag
    18481 attggcatcg gtcaccagcg tggcaatctc aataatgcga tcgcgctcgg gatccagacc
    18541 ggtcatctca agatcgatcc aaatcaggtt gttttcattg gcactcatgc tattttccac
    18601 ccattcacag tgactatatt gatccacaat tgcgtgtatc atagatgttt tgcccatcag
    18661 gggcgaccag gagtcagtac gattgagtaa aaataaactc tccaaaggcc agcagcgccg
    18721 cgtgaacgcc aatcaccagc gtcgtcttaa aacgtctaag gagaagcccg actacgacga
    18781 caatctgttt ggcgagcctg atgaaggtat cgtcatcagc cgctttggta tgcacgctga
    18841 tgtggaatcc gccgatggcg acgttcaccg ctgcaatatt cgccgtacca tccgttcgct
    18901 ggtaaccggc gaccgcgtag tctggcgtcc gggtaaaccg gcggcggaag gcgtaaatgt
    18961 caaagggatc gtggaagcgg tgcatgagcg tacctcggtg ttgacgcgtc cggatttcta
    19021 cgacggcgta aaacctattg ccgccaacat cgaccagatt gtcattgtct ccgccatttt
    19081 gccggagctg tcgctcaata ttatcgaccg ttacctggtg gcctgcgaaa ccttgcagat
    19141 tgagccgatt attgtgctca acaagataga cctgctggac gacgaaggca tggcgttcgt
    19201 caacgagcag atggatatct accgcaatat cggttatcgc gtattgatgg tttccagcca
    19261 tactcaggat gggctaaaac cgctggaaga ggcgttgacc gggcgcatca gcatttttgc
    19321 cgggcagtct ggcgtcggca aatccagcct gctgaacgcg ttgctggggc tgcaaaaaga
    19381 gatcctgacc aacgatgtct ctgacaactc ggggcttggt cagcacacca cgaccgccgc
    19441 tcggctgtat cacttcccgc acggcggtga tgtgattgat tcccctggtg tgcgtgagtt
    19501 cggcctctgg cacctggagc cggaacaaat cactcagggc tttgtcgaat tccatgacta
    19561 tttaggtctg tgtaaatatc gcgattgcaa acacgatacc gatccgggct gcgctatccg
    19621 ggaagcggtt gaggaaggga aaatcgcgga aacccgtttc gaaaactatc accgtattct
    19681 ggaaagcatg gcgcaggtaa aaacgcgtaa aaacttttct gatacggatg actgacaatt
    19741 aagctaaccg tcgttagaat cgtccccttt ttttcaggat ccggcatgta ggccgaatca
    19801 ggaacgacaa aacaatggcc tggaggctac cttgttaaat tcatttaaac tttcgctaca
    19861 gtacattctg ccgaaactat ggcttactcg cctggcgggt tggggcgcaa gcaagcgggc
    19921 aggatggttg acaaaactgg ttatcgatct gttcgttaaa tactacaagg tcgacatgaa
    19981 agaggcgcaa aagccggaca ccgccagcta ccgcaccttt aacgaattct ttgtccgtcc
    20041 gctgcgtgac gaagtacgcc caatcgatac cgatccgaat gtactggtca tgcctgccga
    20101 tggcgttatc agccagttgg gtaaaatcga agaagataaa atcctgcaag ccaaaggcca
    20161 caactacagc ctcgaagccc tgctggcagg caactatctg atggcggacc tgttccgcaa
    20221 cggtacgttt gtgaccactt acctctcccc gcgtgactac caccgcgtac acatgccgtg
    20281 caacggtatt ctgcgtgaga tgatctacgt gccgggcgat ctcttctccg ttaaccatct
    20341 cacggctcag aacgtgccga atctgtttgc ccgtaacgaa cgcgtgattt gccttttcga
    20401 taccgaattt ggcccaatgg cgcagattct ggtcggagcg acgattgttg gcagcattga
    20461 gacggtctgg gcgggcacca ttacgccgcc gcgcgaaggt atcatcaagc gctggacctg
    20521 gcctgccggg gaaaacgacg gttctgtggc actgctgaaa ggccaggaaa tgggtcgctt
    20581 taaactcggt tccaccgtta tcaacctgtt tgcaccgggt aaagtgaatc tggttgagca
    20641 actggaaagc ctgtctgtca cgaaaatttg ccagccgctg gcagtatcta ccgaaacctt
    20701 tgttacgcca gacgctgaac ctgccccgct tcctgctgaa gagatcgaag cagaacacga
    20761 cgccagccca ttggttgacg acaaaaaaga ccaggtctaa tccatcaaag gaaacgctga
    20821 cgtgcgcctg attatcactt ttctgatggc ctggtgcctc agttgggggg cgtacgccgc
    20881 gacggccccc gatagcaaac aaatcactca ggaactggag caggcaaaag cggcgaaacc
    20941 cgcacagccg gaagtcgtag aggcgcttca gtctgcctta aatgcgcttg aggaacgaaa
    21001 aggttccctt gagcgcatca aacaatatca gcaagttatc gataattatc cgaaactctc
    21061 cgctactctg cgcgcacaat taaacaacat gcgtgacaag ccgcgcagcg tgtcgccggg
    21121 aatgtctacc gacgcgctga atcaggaaat tctccaggtc agcagccagt tgctggataa
    21181 aagccgtcag gcccagcaag agcaggagcg cgcccgcgag attgccgatt cgctgaatca
    21241 actgccgcaa cagcaaaccg acgcccgccg tcagttaaat gagatcgagc gccgcctggg
    21301 aacgcttacc ggcaatactc cgctcaatca ggcacaaaat ttcgcgttgc agtctgactc
    21361 tgcacgtctt aaggcgctcg ttgatgaact ggagctggcg cagctgtctg ccaataaccg
    21421 ccaagaatta gcgcgcttac gctcagagct tgcggaaaaa gagagccagc aactggatgc
    21481 gtatttgcag gccttacgta atcagttgaa tagccaacgt cagcttgagg cggagcgggc
    21541 gctggaaagt accgaattac tggcagaaaa cagtgccgat ttgccgaaag atatcgtcgc
    21601 gcaattcaaa attaaccgcg aactatcggc agcgctgaat caacaggcgc agcggatgga
    21661 tctcgttgcc tcgcaacagc gtcaggctgc cagccagacg ttacaggtcc ggcaggcgtt
    21721 gaatacgctg cgtgaacagt cgcaatggct gggatcgtcc aatctgctcg gtgaagcgtt
    21781 gcgagcgcag gtggcacggc taccagaaat gccaaaacca caacagcttg ataccgaaat
    21841 ggcgcagttg cgtgtgcaac ggttacgtta tgaggatctg cttaataaac agccgctgct
    21901 acggcaaatc catcaggccg acggtcagcc gctgaccgcc gagcaaaacc gtattctgga
    21961 agcacaactg cgcactcagc gtgagttgct gaactcattg ttgcagggtg gcgacacgct
    22021 actattggaa ctgaccaagc tgaaagtctc caacgggcaa ctggaggatg cgctgaaaga
    22081 ggtgaacgaa gcaacgcacc gctatctgtt ctggacctct gacgtgcgcc cgatgaccat
    22141 cgcctggccg ctggaaatcg cccaggatct gcgtcgtctc atttcgctgg acaccttcag
    22201 tcagttgggc aaagccagtg tgatgatgct gaccagcaaa gagacaattt tgccgctgtt
    22261 tggcgcgttg attctggtcg gttgcagtat ttactcgcgc cgctatttca cccgttttct
    22321 tgaacgttcg gcggcgaaag ttggcaaagt gactcaggat cacttctggc tgacgttgcg
    22381 cactcttttc tggtcgattc tcgtcgcgtc accgttaccg gtgctgtgga tgacgctggg
    22441 ttacggcttg cgcgaggcgt ggccttatcc gctggcggtc gcgattggcg atggtgtaac
    22501 ggccaccgtg ccgctgctgt gggtagtgat gatttgcgcc acctttgccc gcccgaacgg
    22561 cttgtttatc gctcattttg gctggccgcg cgaacgcgta tctcgtggga tgcgctacta
    22621 cctgatgagc atcgggctta ttgtgccgct gattatggcg ctgatgatgt tcgataacct
    22681 cgacgaccgt gaattctccg gttcgctggg aaggctttgc tttatcctca tttgcggtgc
    22741 gctggcggtg gtcaccctca gcctgaaaaa ggccgggatt ccgctgtatc tcaacaaaga
    22801 aggcagcggc gacaacatta ccaaccatat gctgtggaac atgatgattg gcgcgccgtt
    22861 ggttgccatt ctggcgtcgg ctgtgggtta tctggcaacg gcacaggcgc tgttagcgag
    22921 gcttgaaacc tcggttgcca tctggttcct gctactggtg gtttatcacg ttatccgccg
    22981 ctggatgctg atccagcgcc gcaggctggc gtttgaccgg gcgaagcatc gccgggcaga
    23041 gatgttagcg caacgtgcgc gtggcgaaga ggaagcgcat catcacagta gcccggaagg
    23101 agcaattgaa gtcgatgaaa gcgaagtcga tctcgatgcc atcagtgcgc aatccttgcg
    23161 gctggtgcgc tcaattttga tgttgatcgc cctgctttct gtcattgtgc tgtggtcaga
    23221 aatccattcc gctttcggct tcctcgaaaa tatttcgctg tgggatgtca cctccacggt
    23281 acagggcgta gaaagtctgg agccaattac cctcggtgcg gtgctgattg ccattctggt
    23341 gtttatcatc accacgcagc tggtgcgcaa cctgcccgcg ctgctggaac tggcgatttt
    23401 gcagcacctg gatttaacgc cgggtacggg ctacgccatc accaccatca ccaaatatct
    23461 gctgatgctg attggcgggc tggtcggctt ctcgatgatt ggtattgagt ggtcgaaatt
    23521 gcagtggctg gtcgccgcgc tcggtgttgg tctcggtttt ggtttgcagg aaattttcgc
    23581 caactttatc tctggtctga ttatcctgtt cgaaaaaccg attcgcattg gcgatacggt
    23641 gacaattcgc gatctcaccg gtagcgtgac gaaaattaac acccgcgcca ccaccatcag
    23701 cgactgggac cgtaaagaga taatcgtgcc gaacaaggcg tttattaccg agcagtttat
    23761 caactggtcg ctctctgact cggtcacgcg cgtagtgttg acgattccgg cccctgctga
    23821 tgccaacagt gaagaagtga cggaaatcct gctcaccgca gcgcgtcgct gctcgctggt
    23881 gatcgacaac ccggcaccgg aagtcttcct ggtggatctg caacaaggga ttcagatttt
    23941 cgagctgcgt atttatgccg ctgagatggg tcaccgtatg ccgctacgcc atgagatcca
    24001 ccagctgatt ctggctggct tccatgccca cggtatcgat atgccattcc cgcccttcca
    24061 gatgcgtctg gaaagcctca acggtaaaca aacggggaga acgctgacgt ctgcgggcaa
    24121 aggtcgtcag gcgggaagtt tgtaatgcat gaagctgcca aatgatgtca acgtggctca
    24181 tttagcagct taacatcatc ttcagttatc accggccctg gagagtatca tccatacgca
    24241 ttacttcctg acaactcatc gccggagaga cgatgagttg caggatttca tatgatcagt
    24301 cgagagctaa ctcaggttta ttcgccatgc gactgcaata gttctggtaa atcgccatcg
    24361 ctaacagcga gaagaagaca gggccgccaa tcatccacaa tgtgctgtcc cagtctccgg
    24421 cttcaaccac aggctgaata atggtgaaga cgttcgcaaa tgtcaccacc agtacgacaa
    24481 ccaccatcgc aatcattgcc gacatacgcg ttttgaaaat cacaaatggt ctgtcgagat
    24541 cctgacgtgc tttaaagaat gggaaagcca gcgcgaggaa caggtaagga agcgtcatag
    24601 acacgttcgc catcagcgtc agcttgttaa agaacgcgga tgcggtaccg ccaccaaacg
    24661 aaaccagcag gatgaagaca gtaaccaacc cgcactgcat ccacatggcg atagacggca
    24721 tccccatcgc attcaggcgc gtcatcggtt ccggccacaa cgctttcggc gtcccctgga
    24781 tgatggcttt cagcggtgaa tagcaaagcg taaagaacgc accggtataa gcgaggaaca
    24841 tcgaaagtcc ggtaatacgc gcaaaccata cgcccagcga caatgacgct tcaggtgaca
    24901 aatgcagtgc gttacccagc gtcaccccga ggctcttcat cagcacatag gtaatattgc
    24961 caaggttaac ggaaccatta cttaatacct gctgccagtt tgtgctgacg ccccataaaa
    25021 atattgccag cgaataaccg attgaaataa caatagcggc aaaaacaata cctttggcaa
    25081 agttcttttc tggattttcc gttttatcga ccagaccacc aaccgcttca attccgccat
    25141 aggcaaaaat agcaaatacc acaaacgata gcattgccag accggactga taacccgggt
    25201 tcggtgatgc aaggaaatta atatcctgcg cgaaatgccc accatttaat aacaaaatag
    25261 taatgcttac taacagcaat actaaattca gacacattac tgcaataccg cccaccgcag
    25321 taatgcgggc aattttatta atcccctttg aagcaacgac ggtgaccaga atcatccatg
    25381 ctactgccag cagaccaacc acctgcgtag gctccagtcc ggcaatacgc cagtgctggg
    25441 tcatgtcgct accatagagg aatgttgaga acggtaccca aactttcgcg gaggtactca
    25501 ccatccagat gatataagag gaaaaccaca taaacgtacc aatgaaggca aaacgtggtc
    25561 cgacactatt attcatccag gaatagatac caccttcttc tttgcgataa gcagctccca
    25621 tttcagccat cattaag
//
LOCUS       DAZRUF010000033        25348 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZRUF010000033 DAZRUF010000000
VERSION     DAZRUF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25348
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.033"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            67..294
                     /locus_tag="ACHXOL_001091"
     CDS             67..294
                     /locus_tag="ACHXOL_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808131.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(371..613)
                     /locus_tag="ACHXOL_001092"
     CDS             complement(371..613)
                     /locus_tag="ACHXOL_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR6808132.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            966..1886
                     /gene="lpxP"
                     /locus_tag="ACHXOL_001093"
     CDS             966..1886
                     /gene="lpxP"
                     /locus_tag="ACHXOL_001093"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR6808133.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2242..2313
                     /gene="ypdK"
                     /locus_tag="ACHXOL_001094"
     CDS             2242..2313
                     /gene="ypdK"
                     /locus_tag="ACHXOL_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR6808134.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2378..3616)
                     /gene="alaC"
                     /locus_tag="ACHXOL_001095"
     CDS             complement(2378..3616)
                     /gene="alaC"
                     /locus_tag="ACHXOL_001095"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR6808135.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            3993..5690
                     /gene="ypdA"
                     /locus_tag="ACHXOL_001096"
     CDS             3993..5690
                     /gene="ypdA"
                     /locus_tag="ACHXOL_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR6808136.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5705..6439
                     /gene="ypdB"
                     /locus_tag="ACHXOL_001097"
     CDS             5705..6439
                     /gene="ypdB"
                     /locus_tag="ACHXOL_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR6808137.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6452..7309
                     /locus_tag="ACHXOL_001098"
     CDS             6452..7309
                     /locus_tag="ACHXOL_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR6808138.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7312..9807)
                     /gene="ptsP"
                     /locus_tag="ACHXOL_001099"
                     /pseudo
     CDS             complement(7312..9807)
                     /gene="ptsP"
                     /locus_tag="ACHXOL_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(9832..10869)
                     /gene="ypdE"
                     /locus_tag="ACHXOL_001100"
     CDS             complement(9832..10869)
                     /gene="ypdE"
                     /locus_tag="ACHXOL_001100"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR6808139.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10869..11954)
                     /gene="ypdF"
                     /locus_tag="ACHXOL_001101"
                     /pseudo
     CDS             complement(10869..11954)
                     /gene="ypdF"
                     /locus_tag="ACHXOL_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(11969..13216)
                     /locus_tag="ACHXOL_001102"
     CDS             complement(11969..13216)
                     /locus_tag="ACHXOL_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFR6808140.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMLGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13238..13564)
                     /locus_tag="ACHXOL_001103"
     CDS             complement(13238..13564)
                     /locus_tag="ACHXOL_001103"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR6808141.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13784..14749)
                     /gene="glk"
                     /locus_tag="ACHXOL_001104"
     CDS             complement(13784..14749)
                     /gene="glk"
                     /locus_tag="ACHXOL_001104"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR6808142.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14953..16209
                     /locus_tag="ACHXOL_001105"
     CDS             14953..16209
                     /locus_tag="ACHXOL_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR6808143.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16324..16650
                     /gene="ypeC"
                     /locus_tag="ACHXOL_001106"
     CDS             16324..16650
                     /gene="ypeC"
                     /locus_tag="ACHXOL_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR6808144.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16791..18029)
                     /locus_tag="ACHXOL_001107"
     CDS             complement(16791..18029)
                     /locus_tag="ACHXOL_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR6808145.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18365..19567
                     /gene="nupC"
                     /locus_tag="ACHXOL_001108"
     CDS             18365..19567
                     /gene="nupC"
                     /locus_tag="ACHXOL_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR6808146.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19617..21805)
                     /locus_tag="ACHXOL_001109"
                     /pseudo
     CDS             complement(19617..21805)
                     /locus_tag="ACHXOL_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(22014..22089)
                     /locus_tag="ACHXOL_001110"
     tRNA            complement(22014..22089)
                     /locus_tag="ACHXOL_001110"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22054..22056),aa:Ala,seq:ggc)
     gene            complement(22129..22204)
                     /locus_tag="ACHXOL_001111"
     tRNA            complement(22129..22204)
                     /locus_tag="ACHXOL_001111"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22169..22171),aa:Ala,seq:ggc)
     gene            22425..22784
                     /locus_tag="ACHXOL_001112"
     CDS             22425..22784
                     /locus_tag="ACHXOL_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR6808147.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22786..23178
                     /locus_tag="ACHXOL_001113"
     CDS             22786..23178
                     /locus_tag="ACHXOL_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR6808148.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23230..24645)
                     /gene="gltX"
                     /locus_tag="ACHXOL_001114"
     CDS             complement(23230..24645)
                     /gene="gltX"
                     /locus_tag="ACHXOL_001114"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR6808149.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            24904..24979
                     /locus_tag="ACHXOL_001115"
     tRNA            24904..24979
                     /locus_tag="ACHXOL_001115"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24937..24939,aa:Val,seq:tac)
     gene            25024..25099
                     /locus_tag="ACHXOL_001116"
     tRNA            25024..25099
                     /locus_tag="ACHXOL_001116"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25057..25059,aa:Val,seq:tac)
     gene            25146..25221
                     /locus_tag="ACHXOL_001117"
     tRNA            25146..25221
                     /locus_tag="ACHXOL_001117"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25179..25181,aa:Val,seq:tac)
     gene            25226..25301
                     /locus_tag="ACHXOL_001118"
     tRNA            25226..25301
                     /locus_tag="ACHXOL_001118"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25259..25261,aa:Lys,seq:ttt)
BASE COUNT         6311 a         6700 c         6230 g         6107 t
ORIGIN      
        1 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
       61 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
      121 tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca
      181 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
      241 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
      301 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
      361 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
      421 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
      481 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
      541 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
      601 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
      661 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
      721 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
      781 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
      841 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
      901 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
      961 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
     1021 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
     1081 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
     1141 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
     1201 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
     1261 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg
     1321 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
     1381 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
     1441 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
     1501 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
     1561 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
     1621 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
     1681 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
     1741 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
     1801 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
     1861 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
     1921 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
     1981 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
     2041 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt
     2101 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
     2161 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
     2221 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
     2281 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
     2341 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
     2401 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
     2461 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
     2521 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
     2581 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
     2641 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
     2701 ggcgtttgta ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
     2761 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
     2821 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
     2881 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
     2941 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
     3001 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
     3061 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
     3121 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
     3181 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
     3241 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
     3301 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
     3361 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
     3421 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
     3481 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
     3541 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
     3601 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
     3661 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
     3721 agaacatttt tatcaatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc
     3781 tacgctaaca agaaaattac caccaatttt attaacatta taatttcaat aaatttacca
     3841 gccagaagtg cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt
     3901 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
     3961 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
     4021 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
     4081 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
     4141 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
     4201 tggtgaacgt acgtattatc gcggtgatgt ccgacgggat tctgtttggc ccgtgggtag
     4261 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
     4321 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc
     4381 tgaaaatccc caaagcacag cgctggcgcg tcggtattct cggcggcatg ttgtgtgaga
     4441 cgttgaccat gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg
     4501 tctctaaaat cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgcttc
     4561 tggtgcaaag cgttgagggc gaaaaagagg ccagcgccgc gcggcaggcc aagctggcgc
     4621 tggatatcgc caacaaaacg ctaccgctgt ttcgccatgt caatagcgag tcattacgca
     4681 aggtctgcga aattatccgc gatgacattc acgccgatgc ggtggcgatt actaataccg
     4741 atcatgtgct ggcctatgtt ggcgtgggtg aacataacta tcagaatggc gatgacttca
     4801 ttagcccgac tacccgtcag gcgatgaatt acggaaaaat catcattaaa aacaatgatg
     4861 aagcccaccg cacaccagag attcattcca tgctggtgat cccattgtgg gagaaagggg
     4921 tcgtgaccgg aacgctgaaa atttactact gccacgcgca tcagatcacc tcgtcattac
     4981 aggaaatggc ggtcggtctg tcgcaaatca tctccacgca actggaggtt tcacgcgccg
     5041 agcagctacg tgaaatggca aataaggcag agcttcgcgc cctgcaaagc aaaattaatc
     5101 cccattttct gtttaacgct ctgaacgcta tttcatcgtc aatccgtctg aatccggata
     5161 ccgctcgcca gttgatcttt aatctgtcgc gttatctgcg ctataacatt gaattaaaag
     5221 acgatgagca aatcgatatc aaaaaagagc tgtatcaaat taaagactat attgccattg
     5281 agcaggcccg ctttggtgac aagctgacgg ttatctatga tattgatgaa gaggtgaatt
     5341 gctgcattcc cagcctgctg atccagccgt tggtggagaa cgccattgtc cacggtattc
     5401 agccttgcaa aggtaaaggc gttgtcacca tcagcgttgc agagtgcgga aatcgggtac
     5461 gcattgcggt gcgagatacc gggcacggca tcgatccaaa ggtgattgag cgggtcgaag
     5521 cgaatgaaat gccgggcaat aaaattggcc tgctgaatgt ccatcatcgc gtgaagttat
     5581 tgtatggcga ggggctgcat atccgccgcc tggagccggg gacggaaatt gcgttttaca
     5641 ttcctaacca acgcacccca gtcgcctcac aggctacgtt attgctttga gccggagtga
     5701 tattgtgaaa gtcatcattg ttgaagacga attcctggca caacaggaac tgagctggct
     5761 aattaaagag cacagccaga tggagattgt cggcaccttt gacgacggtc tggacgtgtt
     5821 gaagtttttg cagcataacc gcgtcgacgc catttttctg gatatcaata ttccgtcgct
     5881 ggatggcgtg ttgctggcgc aaaacatcag ccagttcgcc cataaaccgt ttattgtgtt
     5941 catcaccgcg tggaaagaac atgcggtaga agcgtttgaa ctggaggcgt ttgactacat
     6001 tctcaaaccg tatcaggagt cacgtattac cgggatgctg caaaaactgg aagcggcctg
     6061 gcaacaacag cagaccagca gtacgcctgc cgcgacggta acgcgtgaga atgacaccat
     6121 taatctggtg aaagatgagc gaataatcgt cacgccaatt aacgatatct attacgccga
     6181 agcgcacgag aaaatgacct ttgtctatac gcggcgtgaa tcctacgtaa tgccgatgaa
     6241 cattaccgaa ttttgcagca aactgccgcc gtcgcatttt ttccgctgcc atcgctcatt
     6301 ttgtgtcaat ctgaacaaaa tacgcgaaat cgaaccgtgg tttaataaca cctacattct
     6361 gcgactgaaa gatctggatt ttgaagtgcc ggtcagccgc agcaaagtga aagaatttcg
     6421 ccagttaatg catctttaat cgggaacttt catgaaagca ccaggcttgc ccgccgatca
     6481 acaatttttc gccgatctgt tcagcggcct ggtgcttaac ccgcaactac tggggcgcgt
     6541 ctggtttgcc agccagcctg cctcgttacc ggtgggcagt ttatgtattg attttccccg
     6601 tctggatatc gtgctgcgcg gcgaatacgg caacctgctg gaagcaaagc agcagcgaat
     6661 ggtggaagga gaaatgctgt ttattccggc acgcgcggct aatttaccga tcaacaacaa
     6721 accggtgatg ctgttaagcc tggtgttcgc accgacctgg cttgggttat cgttttacga
     6781 tagccgcacc acgtcgttgt tgcatcctgc tcgccagatc cagcttccca gcctgcaacg
     6841 cggtgaaggt gaagcgatgc ttaccgccct cacccatctt agccgttcgc cgctggagca
     6901 gaatatcatt cagccactgg tgttaagttt gctgcatctt tgccgcaacg tggtgaatat
     6961 gccgccgggt aattcgcaac cgcgcggcga ttttctctat cacagcattt gtaactgggt
     7021 tcaggataat tatgcccagc cgctcacccg cgagagcgtg gcgcagtttt ttaatatcac
     7081 gcccaatcat ctgtcaaaac tgtttgctca gcatggaacg atgcgtttta tcgagtatgt
     7141 gcgttgggtg cgaatggcga aggcgaggat gattttgcag aaatatcatc tgtcaattca
     7201 tgaagtggca cagcgttgcg gttttccgga tagcgactat ttttgtcgcg ttttccggcg
     7261 tcagtttggt ctgacgccgg gagagtacag cgcccgtttt cagggctaac gtcagaaggt
     7321 taattctgtt tccagcagcg tcaggatact ttgtgcatct tgcgcggcaa acaacgactg
     7381 gcggaagttt ttattcacca gtttacgcgc cagctgcgag aagactttca catgattcat
     7441 cccttcgtta gcacccagcg tcagcatgat caccagttca acttcgccca tttctgactg
     7501 ccagtcaacc ggtttcgcca gccgggcaat gctgatactg gaatgacgga tccactgaga
     7561 tttggtgtgc gggatcgcta cgccaaaacc aacgccggtg gtaacaatct cttcccgctg
     7621 ccagacatct tcttccagct caaacggatg ttcagtgcgc ccgttaacgc cgaggttgcc
     7681 gcacaggaac tggatcgcct gctctttatt gctaaaatcc tgatcaacaa agatattctc
     7741 cagcgccagc agtgggcgaa cgtcttcttc cggcgtaaag gcggtgagta acgcttcaat
     7801 ttcctgcgca ctgcggcatt cacatgcctg acgcgccagt tcccgacacg cctcgctatc
     7861 cagttgacga agctggcttt tcaccgccgg aatacgcggg ctactcatac tcagctcatc
     7921 caggcccagc ccaagcagta gcggcagata acggctttca ccgcccagtt caccgcaaat
     7981 gcctacccat ttgccccgct gatgcgcagt ggtaactatt tgctgcaaca tgcgcaggaa
     8041 cgatggcgta atcgggttat atagcggcga tacgcgcggg ttattacgat cgaccgcata
     8101 cagatactgg gtcatatcgt tggagccgat actgaagaaa tcgacctcat cggagaagtg
     8161 gtcgatgatg tagcacaccg acggcacttc caccatgatc ccaagcgtaa tcgtctctgc
     8221 atgacgcagg ccatcgcgct taagctcaac gatcgctttt tggatctcgc ctttcaccca
     8281 taagatctga tcgaggctgt gaaccatcgg gatcatcaac tgggcgttgc cgaaactggc
     8341 ggcgcgcaaa atggcccgca gttgagtgcg gaacaggcca gcaaattccg ggtaaatacg
     8401 taccgcgcga tagccgagga acgggttctc ttcctgggga atattcagat aaggaatgct
     8461 tttatcgccg ccgatgtcca tcgtgcggaa gataatcggc ttgtcgcccg ccgccagtag
     8521 cacctgctgg taggcttcaa attgctcctg ctcgtccggc gcgctgtcgc gatccatata
     8581 gagcatttcg gtacggaaca gcccgacacc ttccgcgccg ttggcaaacg cgcctggcgc
     8641 ttccagagcg gtgccgatat tcgccgcaat atcaatacgc ttgttatcac gggaataggc
     8701 cagctgcgcg gcagcctgcg cctgttgttt ttggcgttta tccgccagcg tctgcgcgac
     8761 ctgataataa ccgctcaccg cgtcattcgg gttaatcgcc agcacgccgc actgggcgtc
     8821 aagcactgca ggttgcccgg cataacgggc aatcgcatcc agcggcaagc cactcagtac
     8881 cgggatcgcc gaggcacggg ccagaatcag tgtatgcgag gtgcgcccgg ttttctccag
     8941 aatcatgccc gcaagatttt tcaaatcaag gctcaaaaac tggcttgggg ttaaatcttc
     9001 agccaccaga atggtcggtt tttccagcac cagattgttg cgcggcttca gttccggcta
     9061 ggtgatatgc agcaactgtt cgctgatatc gcgaatgtcg ctgacccgct cgcgcagata
     9121 atcgctggca gaagcagata gtttggcgca aacctgctcc atattgctga tgatcgccgc
     9181 ccccagtccc tgatgctgtt ctgtcatcag gcgacggata ttgcctgcaa attcatcatc
     9241 ctgaatcagc gacaaatgag cgctgaggat agttttgctt tcgccgtcac gctcacgcaa
     9301 ttgctggttc agttgctcgg caagcgttgc caggctgtgc tccagccggg tggaatcttg
     9361 cgcactggcg gggattgccc gataactgtc gaggctgtcg ctttgtaaca gggtcagcgt
     9421 acccacgccg acgccgcttg ccagcacatt gccgtacagt aaatccgggt taaggcggct
     9481 taatgaacgc ggcagcggat gcgccgtcag ttctgcctgc gtcggctgaa cgctgtcgct
     9541 gtcgataaag cgcaccagga tgtactcttc cagcacgcgc cgcgcctgct cttcatcgct
     9601 gccgctaatg ttcaggctgc aactgtcatt aaataaggtg ccagttccaa tcaacgccag
     9661 cgagctttta gcatcggcct ttgcgttctg gcgatggttg ataaaggtga tttcgctttg
     9721 ccactggctg cactgttctt taagttccca cgccggacga gcatgtagac cgttaggcaa
     9781 aggacagaga aattgaatcg ttaacataac cgctccgggt taatcaggag atcatctgaa
     9841 atccgtcagt tgaacaaccg tctcacgcgt aagacgttga ataagggcag ataaaagttg
     9901 ctccatctgc aaaatgtcgc ggcaatcggc aatcgatgcc gcgcaatgtc cgtggcgggt
     9961 ggcaggcccc attaccactg tgggtacgcc agtatcggtt aagtgcaccg ctccaccgtc
    10021 cgtgccgccg ttactgaaca tatccgcctg caacggaacg ccaatttctg ccgacacggt
    10081 ttcaatccac gcggtaagtt ttggcggcgc aatcagcgat ttatcgctta acaccagcat
    10141 cggcccgtta ccaatctgac gatggttagc cgcgccataa tcaaagtttt tcgcccagca
    10201 ggcggtatca agcacaatgg cgacgtccgg cgacaccgcg cgggtagcag tttgcccgcc
    10261 gcgtaatccc acctcttcgc tggaacttgc caccagccac acttccgcag gcagttcggc
    10321 gtcgtgcagc tcacgtagta acgtcaccag cagatagcaa ccgaggcggt catcaaaggc
    10381 tttccccatc acccgctggt gagggagaac ctgaaaagtt gtatcaaagg tgacgcgatc
    10441 gcctggacga attcccgcct gcatcacttc gtcatacgag cgcgcaccaa tgtccacgcg
    10501 catggcgctg acgtcattcc cctgccggtc gccgtcaagc aggcctggaa ttttgcactc
    10561 ttcacgggtg gtgatgcgca ccggctgcag ctggcgggca gccatgcgca cgttgccaac
    10621 cggcagcaca tcaattgccc cttcgcggga gatgctgcgc accataaatc ccacttcgtc
    10681 catatgcgca cagatcatca ccttcggacc tgtcgattca ttgaggcgga tcagcaccga
    10741 tcccagacca tcaaatcgta cttctttttg caggcgatcc gcttcttcca gtaggatctg
    10801 ccgcacttcc tgttccgagg aggcgatcgc atctgcctcg ctcaacgctt ttaatagcga
    10861 taaatccatt atgcctctcc cgtgagcaac actgttttcg gcatggcgta gagcacttct
    10921 gcgccttgcg gggtaaccag cacaacatct tcgatgcgca cgcccccttg ccctggcaaa
    10981 taaatccccg gctccacggt cagtaacatg cctggctgta gcgtcgtggt atcccgcggt
    11041 gaaaagcgtg gatcttcgtg tacttcaata ccgatagcat gaccggtgtt gtgaccaaaa
    11101 tagtcgccaa atcctgcctc ggtaatcacc cggcgcgcgg cgtcgtcaac ctgttagcag
    11161 cgtacgccag ggcgaatcgc ggagattgcc gccagctgtg cttgcaggac aatctgatag
    11221 acgttaaaca gcgggtgaga ttcggcaatt accccttcgc cattcaccag caaggtgcgc
    11281 gtcatatcag agcagtaccc ctgatagagc gcaccaaaat caagagtgac aaactcgcct
    11341 gctgcaacaa tcttgtcgct ggctttgccg tgcggcagcg ccccacgcca gccgctggcg
    11401 acaatggtgt caaaagaggc tttttctgcg ccctgctggc gcataaacca ctccagttca
    11461 gcggctatct cgcgctcgct catccccgcc tgaataaagc ggcgaatatg ctccgctccg
    11521 cgatcggcaa tcccacaggc gaggcggatt atctccacct cctctggcgt tttgatttgc
    11581 cgcagcacat ccggcgtggc gctaaccagt ttcgcattga gttcagactt ccagcggtgc
    11641 gcggtttccc aactcacctg ctggccttca aaaccgagcg tttgcaactg ctcatcggca
    11701 atgatttgat tgacgatagt ggtaagcgtg ttcgtcgcgt caagcaaatg cagctggtag
    11761 ccttgcgcac gggcttccac ctcaacgaaa tagcgcgaat ctaccagaat gtgcgcactt
    11821 tcacggctaa taaccacata gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc
    11881 tgccgtgagg agagaagcac tgcatccagt tgttgcgcct taagccagtc gcgcagcgaa
    11941 gcgagtaatg tcatctggat tttccttctt acaggctatc gattaacaat ttacctttac
    12001 ggaacatcat cagacgcagg aacaccacca tcagcgcagt tatgactgcc cccagcgcga
    12061 tccccgccat atagacgccc aggttagtga ccagcggcca tgcccaaata gcggattccg
    12121 ggaaccattg cactgcgccc agccagacag cggcggttga gccgacaatc gcgccgacca
    12181 tatacgacgg aatggcggtg atggggcttt ccagcgcaaa cggaatcgca ccctcactaa
    12241 tccccataaa ggcaaggaac atcgcggtct taccctgcgg ataaagttga gcgttgaaca
    12301 ggcgtttgcc ggttaaacga cggtcgataa tggtcgccag acccagacct atcggcggaa
    12361 taacgatagc gatagaacgt gcagtgaccg gtaatacgtg gtcagtggta aagctgaagg
    12421 caacaaaacc tgcggctttg ttgatcgggc caccaaggtc gattgcggtc gccgcggcga
    12481 tccccattgc gtacatcagc gcgcccttct cacctgcggc agtcagcacg gtacggatac
    12541 cgccgttgat ccagccgcca aagggggtaa tgacgtagta catcgccagc atgacaaaaa
    12601 tggcggaaag aatcggtagc aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact
    12661 ggattttctg gttcatccac ttcacaaggt aacccgcgac gatagaaata atcagcgcac
    12721 caatgaaggt ggacggcacc ggcgaagagg tcgcccattg catagtgctg ggatcgaagt
    12781 tcagcagttg cgtcggctgg gtagacatca agccgccgat aaaaccagcc gggaacgcca
    12841 gtttgccacc gatagagttc gccacaaaag cagcaaacat cggaatggca aaaccaaaca
    12901 acacgccgcc gaaggactgc gacagccagg caaatttcag taaggagagg tcgaaaccgg
    12961 agaatttccc gctattaaga gcatccatga tgccgatctc agcaggaatt ttaagccagc
    13021 tataagcaat cagctggctg aaagcgagga tcacaccgcc cataattaag gtcggcacca
    13081 tgcgtgaaat ccctaacatc acatgctgcg ggagttcacc ccagaagctg cttttagagg
    13141 cctgcggatt cttaactgcc gctgccgcac cggatgcgcc aggcacaacg gttgcactgc
    13201 gttttttaat ggccataaca attatccctg tcgaatatta ctgttgttca gaggcaatca
    13261 tctcttcgat ttctttgatg atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt
    13321 cataaacgtc gcgtgattcg aaacgctcat tatcttccgg ggtaactgcc acggagtgga
    13381 tgatgatggt tgcttcggcg atatcctgcg ccgtcaggcg attctggata ccgtccgcgc
    13441 cctgggtttc aattttcact tcataacctg cttctaccgc cgcttcttcc agcgcctgag
    13501 cggccataaa ggtgtgagcc aggcccattg ggcaggcaca taaggcaatt agtttcttac
    13561 tcatcgttaa ttcctcgttc agtgatagtt gacgagtgaa attatgaacg tgcaaaacaa
    13621 atcgccgtta ccagacaaat aatgcattta ctggagaatt aatcccgctg tacggaagtg
    13681 tgacgcaggt cggccttgtg agggggacac aaagaaaaat tgaattaact gtatgattta
    13741 aaagattatc gggagagtta cctcccgata taacagaaag gatttacaga atgtgcccta
    13801 aggtctggcg taaatgtgca ccggaaccga gaagacccgg attgtcatgg acgatgagat
    13861 acaccggaat atcatggaca tattctttaa agcgcccttt atcttcaaat gcggcacgga
    13921 aaccagaggc tttgaagaac tcaaggaagc gcggcacgat accgccagca atataaaccc
    13981 caccaaaggt tcccagcgtt aatgccagat tgccgccaaa acggcccata atgacgcaaa
    14041 acagcgacaa cgcacggcgg caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat
    14101 cttttggctt gagattttct ggcaggcggt tgtcggcttt aacaattgcg cgatacaaat
    14161 tcaccagccc aggaccagaa agcacgcgtt ccgccgaaac atgaccaatt tccgcacgca
    14221 atatttcgag gataatggcc tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt
    14281 cgcccggcaa gcttacccaa cgcttatcga catggaccag atgcgcaacc ccaagccctg
    14341 ttccggcacc gtaaaccgca ataggtttac cttcgaccgg ttctgcgcca ccaaactgaa
    14401 tcagatgctc ttttttcagc atcgggatcg ccatcgatac agcggtaaaa tcgttaataa
    14461 tttccagatg gctaaaaccg agattctttt tcatttcggc aattgagaac gcccaggtat
    14521 ggttggtcat tgccacccag tcaccggtaa ttgggcaagc gatggcaata cagccgtctt
    14581 tcacctcgac cttatgttct tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat
    14641 caagccctga ataggtctta gcctgcgaga tttcaccact ggcaatatca cacagagcaa
    14701 gacgtgcgtt ggtgccgccc acatcaccga ctaatgcata ctttgtcatt cttcaactgc
    14761 tccgctaaag tcaaaataat tctttctcac actgtaaata cctgggggca taacaacaac
    14821 gccggaaagg caggctccct gtaaatatcg atctgggtca cacatttact ttatcgtttc
    14881 agcaccaatt gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat
    14941 aaggaataga acatgctcca tccgcgagcc agaaccatgt tgttattatc gctccccgcc
    15001 gtggcaattg ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta
    15061 ttacagaatt tgctctggca acgactgccg ggaactctgg ggatagccca ggattcacct
    15121 atctggatca tcggtgtatt aacgctaacg ggtattgcgg tggggttggt tatccgtttc
    15181 agccagggtc atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc accagttccg
    15241 ccctctgcgc taccaggact tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc
    15301 ctggggccgg aacatccgat catgaccgtc aatatcgccc ttgccgtcgc cattggcgct
    15361 cgtctgttac cgcgcgtcaa ccgaatggag tggactattt tagcctctgc cggaaccatc
    15421 ggcgcgctgt ttggtacacc tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc
    15481 agtagtgaag ttccgctatg ggatcgtctt tttgcgccgt taatggcggc agcagctggt
    15541 gcacttacta ccggattatt tttccatccc catttttcac tgcccattgc gcattacgga
    15601 cagatggaaa tgaccgatat tctcagcggt gcaattgtcg cggcgattgc catcgcagca
    15661 gggatggtcg ccgtatggtg cttaccacgg ttgcacgcga tgatgaatca aatgaaaaat
    15721 ccggtgctcg tgctgggtat tggcggattt attctcggaa ttctgggggt tattggtgga
    15781 ccagtttcgc tgtttaaagg gctggatgag atgcagcaga tggtggcaaa tcaggctttc
    15841 agcaccagcg attacttttt gctggcggta attaaacttg ccgccctggt cgttgctgcc
    15901 gccagtggct ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg
    15961 ttgatgctgc atgagcacgt tcccgccgtt ccagcggcaa taaccgtttc ctgcgctatt
    16021 ctcggcatcg tgctggtggt aacacgcgat ggctggttaa gtctttttat ggcggcagtc
    16081 gttgtaccca ataccacatt gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg
    16141 ttattagcag gtaagccgat gatgatggtc aatcgtccga agcaacagcc accccacgat
    16201 aatgtttagc aaaaaatgct ctcctttatg atttaagagt tatggcgatg atacggggct
    16261 ttacctcccc gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg aaggagaata
    16321 acaatgttca ggtcactgtt tctggcggcc gccctgatgg catttacccc gcttgcagca
    16381 aatgcaggtg aaatcaccct actgccatcg atcaaattac aaattggcga tcgcgatcat
    16441 tacggtaatt actgggacgg tggtcactgg cgagatcgtg attactggca tcgcaattat
    16501 gaatggcgca aaaaccgctg gtggcgtcat gataatggct atcatcgtgg ctgggataag
    16561 cgtaaagcat atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga
    16621 aaaggccgcg gacatgggca ccgccattaa acgcgtcctc caatggagca caatgcctga
    16681 tgcgacgcta gcgcgtctta tcaggcctac aaaccggctc tttaacgcgt agggcggata
    16741 aggcgtttac gccgcatccg ccaatagtgc cagatgcgac gctcattcaa ctacaatccc
    16801 agcgccgtcc ccaccaacaa ccagatattc agcgccacga ccagcactac aatcacccag
    16861 cctgtctgtt ttacgcgttt gctgttcacc agatcgccca tcaacttgct gtcactggtg
    16921 aaaatcagca gtggaaccag cgccagggcg ataccaaaac ttaacagcac ctgactcata
    16981 accagaatcc gtgtcggatc taatcccatc agaatgacaa taaatgacgg caacatggtg
    17041 actgtacgac gcacccacag cgggatatgg aagcgaatga atccctgcat caccacctgc
    17101 cccgccagtg tccccaccac cgttgaggac agtccggcag caaccagact taacccaaag
    17161 accgttgccg cagcatggct taacagcggt tgcagcgtca gataagcctc atcaagatcg
    17221 gcaacaccag tatgaccaga aaagtggaac gccgccgcag ctgtagccat catcgccaga
    17281 ttgacaaaac cggcaatcgt catggcgata gccacatccc atttggtggc ggaataacgt
    17341 tgttgacgcg aaccgccatg taaatgctga gtgagcgaag agtgcaaata aatcacatgc
    17401 ggcataatcg tcgcccctaa cacgcctgct gccaggaaga ccgcctccga agtaggtaaa
    17461 ctcgggatca ccattccttt acccagctgc gccaggttag gctgggagaa aatcaactcg
    17521 acaatgtaag ccgcggcaac aaacaacagt aacccgccaa tcactttctc cagtggtttt
    17581 tgcccgcgac gttgcagcat taaaatcagg aaagtcgcga tccccgtcag caccgcgccc
    17641 tgcaacaacg agacaccaag aatgagttta aaaccgatcg ccgcaccaat aaattccgcc
    17701 aggtcggttg ccatcgcaat aatttctgcc tgaacccaat agaaccacac tacgggacgc
    17761 ggatagtgat cacgaatctg ctccgccaga ttttttccgg tggcaatccc tagtttggca
    17821 gagaggatct gaatcagcat cgccatcagg ttggcccaaa cgacaaccca cagtagctga
    17881 tagccgaagc tggcacccgc ctgaatattg gtcgcaaagt taccgggatc gatataacca
    17941 atcgccgcaa tgaacgcagg tcccattaat gcgagcctca tcttgcgcgc cgcccgtccg
    18001 ctgctactct caacgcgata gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg
    18061 tttcatgcta tgataaacga gaatgattat caaattcatt taaatggatt gtggtgattt
    18121 ctctgataga ccaggattat gactacgaaa agattgatga cttgaatgtt atggttatgt
    18181 ttaatgttag cacatttaca taactttcag cttccataca caacatagca gaaatgtatg
    18241 acagatcact atttttgaag cctgtcacag gacgtcatta tagtgtgtgt cagatctcgt
    18301 tttccttaac catgttacat agaatgtgca cggaaattta acctgcctca tatttggagc
    18361 aaatatggac cgcgtccttc attttgtact ggcacttgcc gttgttgcga ttctcgcact
    18421 gctggtaagc agcgaccgca aaaaaattcg tatccgttat gttattcaac tgcttgttat
    18481 cgaagtgtta ctggcgtggt tcttcctgaa ctccgacgtt ggtttaggct tcgtgaaagg
    18541 cttctccgaa atgttcgaaa aactgctcag atttgccaac gaagggacta acttcgtctt
    18601 tggtagcatg aatgatcaag gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt
    18661 ctttatctct gcactgatcg gtattctcca gcacattcgc gtgttgccgg tgattatccg
    18721 cgcaattggt ttcctgctct ccaaagtcaa cggtatgggc aaactggaat cctttaacgc
    18781 cgtcagctcc ctgattctgg gtcagtctga aaactttatt gcctataaag atatcctcgg
    18841 caaaatctcc cgtaatcgta tgtacaccat ggctgccacg gcaatgtcca ccgtgtcgat
    18901 gtccatcgtt ggtgcataca tgaccatgct ggaaccgaaa tacgtcgttg ctgcgctggt
    18961 actgaacatg ttcagcacct ttatcgtgct gtcgctgatc aatccttacc gtgttgatgc
    19021 cagtgaagaa aacatccaga tgtccaacct gcacgaaggt cagagcttct tcgaaatgct
    19081 gggtgaatac attctggcag gtttcaaagt tgccattatc gttgccgcga tgctgatcgg
    19141 ctttatcgcc ctgatcgccg cgctgaacgc actgtttgcc accgttactg gctggtttgg
    19201 ctacagcatc tccttccagg gcatcctggg ctacatcttc tatccgattg catgggtgat
    19261 gggtgttcct tccagtgaag cactgcaagt gggcagtatc atggcgacca aactggtttc
    19321 caacgagttc gttgcgatga tggatctgca gaaaattgct tccacgctct ctccgcgtgc
    19381 tgaaggcatc atctctgtgt tcctggtttc cttcgctaac ttctcttcaa tcgggattat
    19441 cgcaggtgca gttaaaggcc tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct
    19501 gaaactggtt tacggctcta ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt
    19561 gctgtaaaac catacataaa aaagccgggg atcattcccc ggctttttta cgcctgttaa
    19621 tcagctaatg gctgcgggcg accaatcaaa tacccttgca gatattgcac cccgagctta
    19681 tgcaatagcg cctgctgctg ttgcgtctcg acaaactccg cgaccacact caatgacttc
    19741 gctttcgcca gatcggtaat tgatctcaca atcatcgcat ccagcgtatt cgtgacaata
    19801 tctttcacaa agacgccatc aattttgatg atatcagcct gcaaacgctt taaccgttcg
    19861 tagttggcat atccggtgcc aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc
    19921 tgctcgatgt tgtacatgct gctttctgcg ttagaaaacg cctgctcctc ggtgatctca
    19981 agaatgaccg cctgcgggga gatgtgataa cgcttaaaca gacgaataat ccgcccggca
    20041 atattctttt gcagcagcgt gagcggcatt aaattgactg aaaagcgcgg tccttttttg
    20101 tcgcaagggt gtgttgccag ccacttcaac aaggattcca gcacttgcaa atcaaaacgc
    20161 gcgctaaggt taaactgagc aataaggggc agaaacttat ccggggtcat aatgccgccg
    20221 tcatatttca gtcgcgcgag gatctcatca taaccttcac cctctttgtt gcgaattggc
    20281 tgggcgtaga gcagcaaatc accctgatct aacgccgtgc gaatggtatt cagcaatagc
    20341 acctgtttag tggtctgccc ggaaaccatc tcctctctgc tatccagcgc cagcacatga
    20401 tgatgtgcgc aggattgctc cgccagccag cttaactgcc ccaacaaggg ttgcagggtt
    20461 tcctgatttc catcaaaacg cccccaggca gcaccatagc ccatatccag cccggtattg
    20521 ttccagtgaa tttgccgact attcaggatg ttaaccatat gctggagtcg cccttccgtt
    20581 tccggcccgc tcagcactaa cagcagttca ctacccggca attgatataa cttttcgttt
    20641 tcctgcatca acggcagcag cgtacggtaa attgatcgga tacagtgaac gcgcatcatt
    20701 aagccgtaat gacgactcat aaactcaaga ttatcaatgc gcaggcagca aaactcttgc
    20761 ccgcctcttg ctccggcgct tgttccaacg cacggaagtt gggcaggagc gttaacggat
    20821 cggtcagcgc ctgcaaatgc cagcggcgat taagccattc actgcgatga taaatgcgca
    20881 ccatatagag caggcaaacg ctaaaggaaa tcagcaccgc aagaataaat gccagcgaat
    20941 attcggtttc caccccttgc aaaaagttct ggttgtaatt cagaaggcaa agcgttgaaa
    21001 cagcccaggt gagatttaaa aacggatagc gaagcttacc gaccccgagg gtaaagatga
    21061 tgaagaaaac gggtaccagg taaccggcaa taaagtcgtt ttcataaggt gtgcacaaca
    21121 gaagtagaag cacgcttaac gctgccagcc agcttaaggt aaatgcgcgt ttctctttgc
    21181 ccaacgacgg cgcgatatcc ctgcgccaca atatctgcgc aaagtgggga cttacaatca
    21241 tgcgagtgag atagtagaaa agcatgttgt aaataagcac agcggtgaaa aggcttagca
    21301 aatcaacgac cgtgaaaatg gcatccgcat cgccgaaaaa ggtggatatc tttagcggga
    21361 aatcaaagaa gctccccaca agatacatgc tgcatttgat gccgatcggc gtcaccaaac
    21421 caagccagaa cagacgttgc cagacatatc ggctggtcaa tccataacgc caacgcgtcc
    21481 ccagctgcca gcgtaatatt gcgcaggcac acaacacgac aaacgtctgg ctaccgagca
    21541 tgaccgcagt ctgtaaaaaa gagagatgaa agttccacag attggtgcaa aacatcccgc
    21601 ataaaacggg aacgacaccg cgccagccaa agataaacaa gattgacagc attacgcaca
    21661 gtggcatcca cgccagaaag atataactgg aatggataat ggcaagtggc gaaataaaac
    21721 gggatagctg aataagtacc acggtgagcg taaacgctag tgtgaatatc ttgatatttt
    21781 ttatcaggtt atgctccaca aacatgagta cactactctc actgatcggg gttttcaggc
    21841 acacaaataa taagtttatt cgcatcgcga agcaaacagg gggccagcct gttgccgatt
    21901 tttcatccaa tgatgctttc accaggcggc actaacccgg acgtagactg tattacaaaa
    21961 gcggcaaaaa gcagagacaa aaaacccccg ctttgcagcg agggttggaa atttggtgga
    22021 gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc ccagctgagc
    22081 tatagcccca cgatgcgttt acgtaccaaa tttgctgggt gcaaaatttg gtggagctaa
    22141 gcgggatcga accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatag
    22201 ccccgtcacg taaagcttgt cgagttgacg ggcggcatca tatgaattcc gcccgaatgt
    22261 gtcaacggca aattgcaacg tgtagtttca atcgctgaaa aatcaggcaa atgaacaatt
    22321 ttggaaaacc gctcgcattc agtagttatt catgtcacgg tttcctgtaa agtggtgtta
    22381 taaaatgaac tactaataga cccacataca ttcagggaat tgttatgttc aaggagagga
    22441 tgacaccaga tgaacttgcc agactgaccg gctatagccg ccagaccatt aataaatggg
    22501 tacgcaagga gggctggaca acgtcaccaa aacctggcgt ccagggtggc aaagcccgac
    22561 tggttcacgt caatgaacag gttcgtgaat atattcgcaa tgctgaacgt ccagaaggtc
    22621 agggagaagc gcctgccctt tctggtgatg cgccgcttga agtattgctg gtgacactgg
    22681 cgaaagagat gacgccagtt gaacaaaaac agtttacatc cttgcttctg cgggaaggga
    22741 ttatcggatt gttacaacgc ttagggattc gcgatagcaa ataatatgaa aagattacgc
    22801 aataaaatga ccaccgaaga actggctgaa tgcctcggtg tggctaaaca aactgttaac
    22861 cgttggatca gagaaaaagg ctggaaaacg gaaaaatttc ccggcgtgaa aggcggtcgt
    22921 gccagactta ttctggtcga tacgcaagtt tgcgagttta ttcagaacac gccagccttc
    22981 cataacacac caatgttgat ggaagctgaa gagagaattg ctgaatacgc tcctggagca
    23041 cgcgccccgg cgtatcgaca aatcatcaac gcgatagata atatgacaga cattgagcag
    23101 ggaaaagtcg cgcaattttt gtcacgtgaa ggaattcgca atttcctcgc ccgtctcgat
    23161 attgacgaat cagcataaat aaaaaacggc aggatattat ctcctgccgt ttatcttttt
    23221 acacgctaat tactgctgat tttcgcgttc agcaataaaa tccagcgctt tgttgatacg
    23281 ctcgatactg cgggtcttac caattgcgtg aacggtaaca tccagtgctg gagactgccc
    23341 cgccccggtt acggcgacac gcagcggcat accaacttta cccataccca cttccagctc
    23401 atccgccgtc gcctgaatag cgtgatgaac gttttcagcg gtccagtcag taatcgcggc
    23461 cagtttgtca cgaaccactt ccagcggctg acgcgctacc ggacgcagat gttttttcgc
    23521 cgcatcggca tcgaactcag caaaatcttc gtagaaataa cggcagctct gtgccatctc
    23581 tttcagcgtc ttgcagcgtt cgcccagcag tttcaccaga tcagccagct gcgggccgtt
    23641 acgggtatcg atattttcct gctcaatgtg ccactgtaag tgagtagcaa catactccgg
    23701 cggcagcgcg ttaatgtagt gatggttcag ccacagcagc ttgtcggtgt tgaacgcact
    23761 ggcagatttg ctgacggcat tcaaagtgaa gtatttgatc atctcttcac gagtgaagat
    23821 ttcctgatcg ccgtgggacc agcccagacg caccagatag ttcagcagtg cttctggcaa
    23881 ataaccgtca tcacgatact gcattacgct gactgccccg tgacgtttgg acagtttttt
    23941 accgtcatcg ccattgatca tagaaacgtg cgcgtaaacc ggcaccggcg ctttcagggc
    24001 cttaagaatg ttgatctggc gcggcgtgtt gttgatatgg tcttcgccac ggataacgtg
    24061 ggtgatttcc atatcccagt catcgacaac cacacagaag ttataggttg gggaaccatc
    24121 ggtacggcgg ataataagat cgtccagttc ctggttgctg aactcgatcg gaccacggat
    24181 ctgatcgtca aaaacaacag aaccttcctg cgggttagca aaacgtacaa cacacggttc
    24241 atcatcagca tgatgctcat ggctgtggcg gcagcgaccg tcataacgcg gcttctcacc
    24301 tttcgccatt tgctcttcgc gcagcgcttc caggcgctct ttagagcaat agcatttata
    24361 agcagtgccc tcttccagca tctgatcgat caccgcgttg tagcgatcaa aacgtttggt
    24421 ctggtagtac ggaccttcat cccactccag gctcagccag ttcatgccat ccataatggc
    24481 ttcgatagct tccggcgtgg aacgctcaag atcggtgtct tcaatacgca gcacgaactc
    24541 accgccgtgg ttacgtgcaa aaagccagga gtaaagagca gtacgcgcgc cgccaacgtg
    24601 cagatagcct gttgggcttg gcgcgaagcg agttttgatt ttcatgaaat ggccttacgt
    24661 ttagaaagat gccgacaacc ggcaaatcct ggaaaaattg agtgggcgat attctatcac
    24721 tcccgcctga ttcctcaatg tagttcgggt ttctacctta cgcattattg ttttcgttga
    24781 caaattgcgc aacacggtct gttttgcgat catcttgctt aaatttacga cgaacgaaca
    24841 atttctttag aaaatgcgtt gactcatttt gaactctccc tataatgcgg ctccacacag
    24901 cgggggtgat tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg
    24961 atcccgtcat cacccaccaa ctactttatg tagtctccgc cgtgtagcaa gaaattgaga
    25021 agtgggtgat tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg
    25081 atcccgtcat cacccaccaa tttctcgcca gctaaatttc ttgtaaaaat gtgaagtacc
    25141 gaagtgggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    25201 cgatcccgtc atcacccacc acttcgggtc gttagctcag ttggtagagc agttgacttt
    25261 taatcaattg gtcgcaggtt cgaatcctgc acgacccacc aatgtaaaaa agcgccctaa
    25321 aggcgctttt ttgctatctg cgatactc
//
LOCUS       DAZRUF010000034        25285 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZRUF010000034 DAZRUF010000000
VERSION     DAZRUF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.034"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            18..269
                     /locus_tag="ACHXOL_001119"
                     /pseudo
     CDS             18..269
                     /locus_tag="ACHXOL_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="involved in type II protein secretion; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            507..818
                     /gene="rpsJ"
                     /locus_tag="ACHXOL_001120"
     CDS             507..818
                     /gene="rpsJ"
                     /locus_tag="ACHXOL_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR6808150.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            851..1480
                     /gene="rplC"
                     /locus_tag="ACHXOL_001121"
     CDS             851..1480
                     /gene="rplC"
                     /locus_tag="ACHXOL_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR6808151.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1491..2096
                     /gene="rplD"
                     /locus_tag="ACHXOL_001122"
     CDS             1491..2096
                     /gene="rplD"
                     /locus_tag="ACHXOL_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR6808152.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2093..2395
                     /gene="rplW"
                     /locus_tag="ACHXOL_001123"
     CDS             2093..2395
                     /gene="rplW"
                     /locus_tag="ACHXOL_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR6808153.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2413..3234
                     /gene="rplB"
                     /locus_tag="ACHXOL_001124"
     CDS             2413..3234
                     /gene="rplB"
                     /locus_tag="ACHXOL_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR6808154.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3251..3529
                     /gene="rpsS"
                     /locus_tag="ACHXOL_001125"
     CDS             3251..3529
                     /gene="rpsS"
                     /locus_tag="ACHXOL_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR6808155.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3544..3876
                     /gene="rplV"
                     /locus_tag="ACHXOL_001126"
     CDS             3544..3876
                     /gene="rplV"
                     /locus_tag="ACHXOL_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR6808156.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            3894..4595
                     /gene="rpsC"
                     /locus_tag="ACHXOL_001127"
     CDS             3894..4595
                     /gene="rpsC"
                     /locus_tag="ACHXOL_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR6808157.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4608..5018
                     /gene="rplP"
                     /locus_tag="ACHXOL_001128"
     CDS             4608..5018
                     /gene="rplP"
                     /locus_tag="ACHXOL_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR6808158.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5018..5209
                     /gene="rpmC"
                     /locus_tag="ACHXOL_001129"
     CDS             5018..5209
                     /gene="rpmC"
                     /locus_tag="ACHXOL_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR6808159.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5209..5463
                     /gene="rpsQ"
                     /locus_tag="ACHXOL_001130"
     CDS             5209..5463
                     /gene="rpsQ"
                     /locus_tag="ACHXOL_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR6808160.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5628..5999
                     /gene="rplN"
                     /locus_tag="ACHXOL_001131"
     CDS             5628..5999
                     /gene="rplN"
                     /locus_tag="ACHXOL_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR6808161.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6010..6324
                     /gene="rplX"
                     /locus_tag="ACHXOL_001132"
     CDS             6010..6324
                     /gene="rplX"
                     /locus_tag="ACHXOL_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR6808162.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6339..6878
                     /gene="rplE"
                     /locus_tag="ACHXOL_001133"
     CDS             6339..6878
                     /gene="rplE"
                     /locus_tag="ACHXOL_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR6808163.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            6893..7198
                     /gene="rpsN"
                     /locus_tag="ACHXOL_001134"
     CDS             6893..7198
                     /gene="rpsN"
                     /locus_tag="ACHXOL_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR6808164.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7232..7624
                     /gene="rpsH"
                     /locus_tag="ACHXOL_001135"
     CDS             7232..7624
                     /gene="rpsH"
                     /locus_tag="ACHXOL_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR6808165.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7637..8170
                     /gene="rplF"
                     /locus_tag="ACHXOL_001136"
     CDS             7637..8170
                     /gene="rplF"
                     /locus_tag="ACHXOL_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR6808166.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8180..8533
                     /gene="rplR"
                     /locus_tag="ACHXOL_001137"
     CDS             8180..8533
                     /gene="rplR"
                     /locus_tag="ACHXOL_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR6808167.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8548..9051
                     /gene="rpsE"
                     /locus_tag="ACHXOL_001138"
     CDS             8548..9051
                     /gene="rpsE"
                     /locus_tag="ACHXOL_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR6808168.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9055..9234
                     /gene="rpmD"
                     /locus_tag="ACHXOL_001139"
     CDS             9055..9234
                     /gene="rpmD"
                     /locus_tag="ACHXOL_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR6808169.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9238..9672
                     /gene="rplO"
                     /locus_tag="ACHXOL_001140"
     CDS             9238..9672
                     /gene="rplO"
                     /locus_tag="ACHXOL_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR6808170.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9680..11011
                     /gene="secY"
                     /locus_tag="ACHXOL_001141"
     CDS             9680..11011
                     /gene="secY"
                     /locus_tag="ACHXOL_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR6808171.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DATVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11043..11159
                     /gene="rpmJ"
                     /locus_tag="ACHXOL_001142"
     CDS             11043..11159
                     /gene="rpmJ"
                     /locus_tag="ACHXOL_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR6808172.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11306..11662
                     /gene="rpsM"
                     /locus_tag="ACHXOL_001143"
     CDS             11306..11662
                     /gene="rpsM"
                     /locus_tag="ACHXOL_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR6808173.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11679..12068
                     /gene="rpsK"
                     /locus_tag="ACHXOL_001144"
     CDS             11679..12068
                     /gene="rpsK"
                     /locus_tag="ACHXOL_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR6808174.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12102..12722
                     /gene="rpsD"
                     /locus_tag="ACHXOL_001145"
     CDS             12102..12722
                     /gene="rpsD"
                     /locus_tag="ACHXOL_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR6808175.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            12748..13737
                     /gene="rpoA"
                     /locus_tag="ACHXOL_001146"
     CDS             12748..13737
                     /gene="rpoA"
                     /locus_tag="ACHXOL_001146"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR6808176.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            13778..14161
                     /gene="rplQ"
                     /locus_tag="ACHXOL_001147"
     CDS             13778..14161
                     /gene="rplQ"
                     /locus_tag="ACHXOL_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR6808177.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14268..14636
                     /locus_tag="ACHXOL_001148"
     CDS             14268..14636
                     /locus_tag="ACHXOL_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR6808178.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14647..15072
                     /gene="zntR"
                     /locus_tag="ACHXOL_001149"
     CDS             14647..15072
                     /gene="zntR"
                     /locus_tag="ACHXOL_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR6808179.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15128..15346
                     /gene="arfA"
                     /locus_tag="ACHXOL_001150"
     CDS             15128..15346
                     /gene="arfA"
                     /locus_tag="ACHXOL_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR6808180.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15343..15753)
                     /gene="mscL"
                     /locus_tag="ACHXOL_001151"
     CDS             complement(15343..15753)
                     /gene="mscL"
                     /locus_tag="ACHXOL_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR6808181.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(15883..17259)
                     /gene="trkA"
                     /locus_tag="ACHXOL_001152"
     CDS             complement(15883..17259)
                     /gene="trkA"
                     /locus_tag="ACHXOL_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR6808182.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17281..18570)
                     /gene="rsmB"
                     /locus_tag="ACHXOL_001153"
     CDS             complement(17281..18570)
                     /gene="rsmB"
                     /locus_tag="ACHXOL_001153"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR6808183.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQHQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18616..19563)
                     /gene="fmt"
                     /locus_tag="ACHXOL_001154"
     CDS             complement(18616..19563)
                     /gene="fmt"
                     /locus_tag="ACHXOL_001154"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR6808184.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19578..20087)
                     /gene="def"
                     /locus_tag="ACHXOL_001155"
     CDS             complement(19578..20087)
                     /gene="def"
                     /locus_tag="ACHXOL_001155"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR6808185.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20217..21341
                     /gene="dprA"
                     /locus_tag="ACHXOL_001156"
     CDS             20217..21341
                     /gene="dprA"
                     /locus_tag="ACHXOL_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR6808186.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21313..21786
                     /gene="smg"
                     /locus_tag="ACHXOL_001157"
     CDS             21313..21786
                     /gene="smg"
                     /locus_tag="ACHXOL_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR6808187.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            21815..22357
                     /locus_tag="ACHXOL_001158"
     CDS             21815..22357
                     /locus_tag="ACHXOL_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR6808188.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22362..22934
                     /gene="tsaC"
                     /locus_tag="ACHXOL_001159"
     CDS             22362..22934
                     /gene="tsaC"
                     /locus_tag="ACHXOL_001159"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR6808189.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            22939..23757
                     /gene="aroE"
                     /locus_tag="ACHXOL_001160"
     CDS             22939..23757
                     /gene="aroE"
                     /locus_tag="ACHXOL_001160"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR6808190.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            23754..24011
                     /locus_tag="ACHXOL_001161"
     CDS             23754..24011
                     /locus_tag="ACHXOL_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR6808191.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(23987..24541)
                     /locus_tag="ACHXOL_001162"
     CDS             complement(23987..24541)
                     /locus_tag="ACHXOL_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR6808192.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25014..>25285
                     /locus_tag="ACHXOL_001163"
     rRNA            25014..>25285
                     /locus_tag="ACHXOL_001163"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6442 a         5993 c         6618 g         6232 t
ORIGIN      
        1 tgcttcttta atgggcgctt tggatagtaa aagcagaagt cactgtagag gctgaaacgc
       61 ccgcttttaa acaatcaaca caacactatt tcttcaaaaa acagccactc cccgtcgtcg
      121 aatccgtcga ggaggaagat gacccagacg tcgcggttga gaatgctccc tcttcttctg
      181 aagacgaaga aaatactgta aaagaaagcg acgagaaagc cggtttaaga gagcgtgtca
      241 aaaatgcgct gaacgaactt gagagataac ccgaaggctg tttactttac tagcaatacg
      301 cttgcgttcg gtggttaagt atgtataatg cgcgggcttg tcgtagttga cagcaggttc
      361 aatctgaacc tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac
      421 gtaagaacgg ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt
      481 tataaaataa ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag
      541 cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca
      601 ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc
      661 tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc
      721 gtctggttga catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc
      781 tggctgccgg tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga
      841 ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga
      901 agacggcgtt tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt
      961 taaagacctg gctaacgatg gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc
     1021 taaccgtgtg accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg
     1081 tggtctgtgg gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag
     1141 cgttgaactg tttgctgagg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg
     1201 tttcgcaggt accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc
     1261 cttgtctcac cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa
     1321 aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt
     1381 agtacgcgtt gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggggcaac
     1441 cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag
     1501 tattgaaaga cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca
     1561 acgaagcgct ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc
     1621 gtgctcagaa gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag
     1681 gcaccggccg tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga
     1741 cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg
     1801 cgctgaaaag catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt
     1861 tctctgtaga agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg
     1921 aagatgtgct gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc
     1981 tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg
     2041 acaaagtcgt aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg
     2101 tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc
     2161 gatggaaaaa tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat
     2221 caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt
     2281 taaagggaaa gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc
     2341 ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg
     2401 aggagtaata caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta
     2461 gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa
     2521 aacagcaaat ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt
     2581 ggccacaagc aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca
     2641 gttgttgaac gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac
     2701 aaagacggtg aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt
     2761 cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg
     2821 gttggttcta ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt
     2881 tccgctggta cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg
     2941 cgttctggtg aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc
     3001 aatgctgagc atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt
     3061 cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa
     3121 ggtcgtaact ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag
     3181 acccgcagca acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag
     3241 aggataagcc atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa
     3301 gaaggtagag aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg
     3361 ttcaacgatc tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca
     3421 cgttccggta tttgtgaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac
     3481 tcgtacttat cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa
     3541 gagatggaaa ctatcgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt
     3601 gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc
     3661 aacaagaaag cggctgtact ggtcaagaaa gttctggaat ctgccattgc taacgctgaa
     3721 cacaacgatg gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc
     3781 ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc
     3841 accagccaca tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc
     3901 agaaagtaca tcctaatggt attcgcttgg gtattgtaaa gccatggaac tctacctggt
     3961 ttgcgaacac caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc
     4021 tgactaagga actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga
     4081 gcatccgtgt aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag
     4141 acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca
     4201 tcgccgaagt tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc
     4261 agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc
     4321 gtctgggcgc taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg
     4381 cacgtaccga atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg
     4441 actacaacac ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct
     4501 tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc
     4561 agcctaaaaa gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa
     4621 agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg
     4681 atgttagctt cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc
     4741 agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga
     4801 tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag
     4861 gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa
     4921 tggacggtgt tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc
     4981 cgattaaaac cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga
     5041 gaagagcgtt gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct
     5101 gcgtatgcag gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg
     5161 cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa
     5221 atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct
     5281 atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca tcaagcgtac gaccaaactg
     5341 cacgtacatg acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc
     5401 cgtccgctgt ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg
     5461 taatacagta cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc
     5521 tacccatatc cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga
     5581 cctgattttc gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac
     5641 agactatgct gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc
     5701 tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag
     5761 aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca
     5821 ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg
     5881 ttcttctgaa caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc
     5941 gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag
     6001 gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa
     6061 gataaaggta aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattgttgaa
     6121 ggtatcaacc tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc
     6181 atcgttgaaa aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc
     6241 ggcaaggctg accgtgtagg ctttagattc gaagacggca aaaaagtccg tttcttcaag
     6301 tctaacagcg aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact
     6361 acaaagacga agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag
     6421 tccctcgggt cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa
     6481 aactgctgga taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca
     6541 ccaaagcacg caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta
     6601 aagtaactct gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg
     6661 tacctcgtat tcgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact
     6721 acagcatggg tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc
     6781 gcgttcgtgg tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg
     6841 ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa
     6901 gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa
     6961 acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa
     7021 cgctgttctc aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg
     7081 ctgccgtcaa acaggtcgcc cgcatggttt cctgcggaag ttcgggttga gccgtattaa
     7141 ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt
     7201 gtcaccaatt gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat
     7261 gctgacccgt atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc
     7321 caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa
     7381 agttgaaggc gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc
     7441 tgttgtagaa agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga
     7501 tgagctgccg aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt
     7561 tatgactgat cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc
     7621 ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt
     7681 tgacgtaaaa atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg
     7741 tactctcaac gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg
     7801 tgatggttac gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat
     7861 ggttatcggt gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg
     7921 tgcagcggtt aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca
     7981 tcagctgcct gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg
     8041 cgttgataag caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga
     8101 gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa
     8161 gaagaagtaa ggtaacacta tggataagaa atttgctcgt atccgtcgtg cgacccgcgc
     8221 acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg gtacatcgta ccccgcgtca
     8281 catttacgca caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt
     8341 agaaaaagct atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt
     8401 gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg
     8461 ttccgggttc caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg
     8521 ccttcagttc taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg
     8581 caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc
     8641 tccttcacag ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt
     8701 aaagcgcgtg aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg
     8761 attaacgtcg cgctgaataa cggcactctg caacaccctg ttaaaggtgt tcacacgggt
     8821 tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg
     8881 cgcgccgttc tggaagtcgc tggggttcat aacgttctgg ctaaagcata tggttccacc
     8941 aacccgatca acgtggttcg tgcaactatt gatggcctgg aaaatatgaa ttctccagaa
     9001 atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca
     9061 aagactatta aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca
     9121 acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct
     9181 gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg
     9241 cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt
     9301 ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaagtctcgt
     9361 tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg
     9421 ccgaaattcg gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctgtctgac
     9481 ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc
     9541 ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg aagtaacgac tccggtaact
     9601 gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa
     9661 atcgaggaat aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg
     9721 tggcttaggc gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg
     9781 tattggctct tttattccga tccctggtat tgatgccact gtacttgcca aactgcttga
     9841 gcaacagcga ggcaccatca ttgagatgtt taacatgttc tctggtggtg ctctcagccg
     9901 tgcttctatc tttgctctgg ggatcatgcc gtatatttcg gcgtcgatca ttatccagct
     9961 gctgacggtg gttcacccaa cgttggcgga aattaagaaa gaaggggagt ctggtcgtcg
    10021 taagatcagc cagtacaccc gctacggtac tctggtgctg gcaatattcc agtcgatcgg
    10081 tattgctact ggtctgccga atatgcctgg tatgcaaggc ctggtgatta acccgggctt
    10141 tgcattctac ttcaccgctg ttgtaagtct ggtcacagga acgatgttcc tgatgtggtt
    10201 gggcgaacag attactgaac gaggtatcgg caacggtatt tcaatcatta tcttcgtcgg
    10261 tattgtcgcg ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga
    10321 cctgcacttc ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt
    10381 tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg
    10441 tcgtcgtgtt tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt
    10501 aatcccggca atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt
    10561 cgggggcggt actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca
    10621 accgctttat gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc
    10681 gttggttttc aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc
    10741 aggaattcgt ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac
    10801 cctggttggt gcgctgtata ttacctttat ctgcctgatc ccggagttca tgcgtgatgc
    10861 aatgaaagta ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat
    10921 ggactttatg gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa
    10981 gaaggcgaac ctgaaaggct acggccgata attggtcgcc cgagaagtta cggagagtaa
    11041 aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg
    11101 atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat
    11161 tttttcgcat atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt
    11221 ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt
    11281 acatattaaa tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca
    11341 taagcatgcc gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc
    11401 catcctggct gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca
    11461 aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga
    11521 aatcagcatg agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg
    11581 tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc
    11641 gcgcaaaccg atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg
    11701 cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca
    11761 acaacaccat cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg
    11821 gtggttccgg tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag
    11881 agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag
    11941 gtccgggtcc aggccgcgaa tctactattc gtgctctgaa cgccgcaggt ttccgcatca
    12001 ctaacattac tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc
    12061 gcgtataacg cctcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc
    12121 ctaagctcaa gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg
    12181 cgatcgatac caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc
    12241 gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg
    12301 tgctggagcg tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg
    12361 gtgaaaacct gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct
    12421 tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg
    12481 gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg
    12541 agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa
    12601 agccaacctg gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg
    12661 agcgttctga tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt
    12721 aaagcttagt accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg
    12781 cgcctggttg atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta
    12841 gagcgtggct ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg
    12901 ccgggttgcg cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa
    12961 gaaggcgttc aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga
    13021 gttcagggca aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact
    13081 gcagccgata tcacccacga cggtgatgtc gaaatcgtca agccgcagca cgtgatctgc
    13141 cacctgaccg atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt
    13201 tatgtgccgg cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg
    13261 ctggtcgacg catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt
    13321 gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc
    13381 gatcctgaag aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc
    13441 gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg
    13501 atcctgctgc gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa
    13561 gcagaagcta tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa
    13621 acgcctaacc ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga
    13681 ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg
    13741 gatcacaggt taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg
    13801 tcaactgaac cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact
    13861 ggttcgtcat gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt
    13921 tgagccgctg attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc
    13981 ccgtactcgt gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc
    14041 gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc
    14101 gccgatggct tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta
    14161 atctgaagca atgtaaaaaa acccgccccg gcgggttttt ttatacccgt agtatcccca
    14221 cttatctaca atagctgtac tctttttgtt catcccctgg agtatttatg tggttacttg
    14281 accagtgggc agagcgccat atagcagaag cgcaagcgaa aggtgagttt gataacctgg
    14341 caggtagcgg cgaaccattg atactggatg atgattctca cgtgccaccg gaattacgtg
    14401 cggggtatcg cttgctgaag aatgccggtt gcttaccgcc agaacttgag caacggagag
    14461 aagcaattca gcttctggat attctcaaag gtatccgtca cgatgatccg caatatcaag
    14521 aggttagccg tcgattgtca ttactggaat tgaagctgcg acaagctgga ttgagtaccg
    14581 attttttacg cggcgattat gctgacaagt tgttggacaa aatcaacgat aactagtgga
    14641 gtatgtatgt atcgcattgg tgagctggca aaaatggcgg aagtaacacc tgacacgatt
    14701 cgttattacg aaaaacagca gatgatggag catgaagtgc gtactgaagg cgggtttcgc
    14761 ctgtataccg aaagcgatct ccagcgattg aaatttatcc gccatgccag acaactaggt
    14821 ttcagtctgg agtcgatccg cgagttgctg tcgatccgca tcgatcctga acaccatacc
    14881 tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg aagtcgaagc acggatagcc
    14941 gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg atgcctgttg tgggaccgcc
    15001 catagcagtg tttattgctc gattcttgaa gctcttgaac aaggggcgag tggcgttaag
    15061 agtggctgtt gattttttgc actgacagga ttacactcgc gccgttaaat aaccaactgg
    15121 agtttttatg agccgatatc agcatactaa agggcagata aaggataatg cgatagaagc
    15181 attactacat gatcccttat tccgacagcg agtagagaaa aataagaaag ggaaaggcag
    15241 ttacatgcga aaaggaaaac atggcaatcg ggggaactgg gaggccagtg gcaagaaagt
    15301 aaatcacttt tttaccactg gtcttctgct ttcaggcgct tgttaagagc ggttattctg
    15361 ctctttcagc aaatcacgaa tttctgccag taatacttct tctttagttg gtgcaggtgc
    15421 ggcagctggt tcttcttttt tccgattcag tttgttgatt agcttaatcg ccataaagat
    15481 ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg aagacaccgt aatgcatcac
    15541 aacagcaggg atatccccct gcgcatcgcg tagcgtgaca gcaaactgtt taaagtcgat
    15601 cccgccaatt aataagccca gaggtggcat gatgatatcg gcaaccagtg aagatacaat
    15661 cttcccgaat gccgcaccga taatgacacc caccgccaaa tccaccacgt tcccgcgcat
    15721 cgcaaattcg cgaaattctt taataatgct catgttattc tccctatgaa gccgacaacc
    15781 ataagtctaa caaatgttaa gccattttcc tgccaggaat gaatttaatt aatccttgat
    15841 aatcaaaggg aaataataag gcgtcattag acgccttatt aattacaaga agaaagggct
    15901 tggctggaag agtctttcga cgtcggtaat aaacttttta tctgtgagga acataattac
    15961 gtgatcgcct tgctcaatgc gcagattgtc attggcaatc atcacgtcgt ttccacgtac
    16021 caccgctcca ataatcgttc ctggcggtag cttgatttcg tcaatgactc tgccgacaac
    16081 gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata gcttctgcta cgccgcggcg
    16141 caatgaggaa acaccaacaa tatctgcttt tcgcacatgg ctaagcaacg cagaaatagt
    16201 tgcttgttgt ggtgaaattg caatatcgat cacgctcccc tgaaccagat ccacataagc
    16261 gcgacgctgg atcaatacca tcaccttttt cgcacccata cgtttggcaa gcatggcgga
    16321 catgatattg gcctcgtcat cgttggtgac ggcaataaac agatcaactt gatcgatatg
    16381 ttcttcggcc agcagttctt gatccgacgc atcaccaaaa aagacgatcg tattctgcaa
    16441 cttttccgcc agttcggcag cgcgctgctg attacgttcg atgagtttaa cgctgtaatc
    16501 tttttccaga cgacgcgcca gccctgcacc gatattaccg ccaccaacca gcatgatccg
    16561 cttatacggt ttttccagtc gctgtaattc actcatcacc gcgcggatat gctgtgaagc
    16621 ggcaataaag aacacttcat caccagcttc aacaatggtc gaaccttgtg gacgaatggg
    16681 gcgatcgtgg cggaaaattg ctgccacacg agtatcgata tgtggcatat gttcgcgcat
    16741 ggtcgaaagt gcattaccaa tcagcgggcc gccataatag gctttaacta cagcgaggct
    16801 gactttaccc tcagcgaagt tcaccacctg caatgcgcct ggatactcaa tcagtcggta
    16861 aatattatcg ataaccaact gctctggtgc gatcagatga tcaatcggta cagcatctga
    16921 atgaaatagc ttatcggcat cgcgtacgta gtctggtgag cggatgcgag cgatgcgatt
    16981 aggggtgttg aaaagtgagt aggctacctg gcaggcaacc atattggttt catctgaact
    17041 ggttacagca accagcatat cggcgtcgtc ggcacctgcc tcccgcaata cgcgtggatg
    17101 agagccatgc ccctgcacga ctcgcaggtc aaatttatcc tgcaaggtcc gcagacgctc
    17161 accgttggta tcgacaacag taatatcgtt gttctcgcca accaggtttt ccgccagtgt
    17221 gccgccaacc tggccggcac ccagaatgat aattttcatc agtctcgacc cgttatctca
    17281 tcactttttg attagcttag cgtaaaagaa gccgtcgccc tcttcggcac caggcagatt
    17341 ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt tcggcatcag cggtacgttg
    17401 caaaaaagct ttaatctgca ggctattctc ttccggtaac accgaacagg tggcatagac
    17461 cagggttcca ccggatttta aatgcggcca aatggcgtcg agaatttcag actgcaattg
    17521 cgcgagttcc gggatatcgc gatcgcgacg taaccattta atatccggat ggcgacgaat
    17581 cacccccgtt gctgaacaag gcgcatctaa taaaatgcga tcaaactgtt gctcgccaca
    17641 ccattgggaa gggtaacggc catcaccttg tttcacggtc gccttcatac caaggcgttt
    17701 taaattgtcg tgaacgcgag agaggcgctg ttcgtcaata tcaaccgcaa caacctgcgc
    17761 ttctggtgcc acctcaagga tatgcgttgt tttaccgccg ggggcggcac aaagatccag
    17821 aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa ccttgtgctg atgcatcctg
    17881 aacggtaacc catccgtctt caaaaccagg tagcgcatga acaggtgcag gtgtttccag
    17941 ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc attcctgctt catccaacaa
    18001 tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg cgcagccaca ttggcggacg
    18061 ctggttattg gcttcgacga tggattgcca ctgctctgga tacgcttttt gcagacgctt
    18121 cagcaaccag gaaggatgca gataacgtgc atcactggta ttaaactcgg ctaataactc
    18181 ttcttgctga tgctggaact ggcgtaatac accgttaatc aaccctttaa gttgcggacg
    18241 cttaattgcg atagcgcctt caaccgtttc agccagcgca gcatgaggtg gaatgcgggt
    18301 ataaagcagt tgatacaaac caaccataat caggtaatgc acagtgcgct gtttgccggt
    18361 catcggacgg gccattaact tattaatcag ccagtctaac tgcgaaagcg tacgcagtac
    18421 gccaaagcac aactcttgaa gaagtgcttt gtctttatca gaaacttttt gctggagcgg
    18481 tggcagaatg ttgcttaatg attgcccttg ctcgacgact tgttcaacgg cctgggccgc
    18541 catgctacgt aaattacgtt gttttttcat aagtataaaa atgcccggta agaccgggct
    18601 tagaagagtg gactatcagg ccagacggtt gcccggaaca aaccattccc gacgagaatt
    18661 caggaggtct tgcgcgctca tcgctttctt acccgcaggt tgtaacgaga gcaggttcag
    18721 gatgccatca ccagtcgcaa cctgaatgcc ttgtttgttg gcttcaagga tcgttcctgg
    18781 tgcagcgttg gttgccgtat caatgaccga cgctttccag actttaaccg gttgtccttc
    18841 aatttccagc cagctcattg gccatgggtt gaaagcgcga atgcagcgtt caagctgcgc
    18901 tgccgaaagc gaccagtcaa tacgcgcttc ttctttactc aacttctcgg cgtaagtgac
    18961 aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct gctagttgtt tcaacgtggt
    19021 gattagccct tgtgggccaa gctctgccag cttgtcgtac agcgtaccac tggtatcttc
    19081 tgcagtaatc gggcaggaga gcttatagag catatcaccg gtgtctaaac cgacatccat
    19141 ttgcataatg gtcacaccag tttctgcatc acccgcccat agtgagcgtt ggattggtgc
    19201 agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg atacagccaa gacgcggcat
    19261 ctccagcact gctttaggca gaattaagcc ataggcgacg acgaccataa catcagcctg
    19321 cagatcggcg accagttgct ggctttcttg tggacgcagg gaaacaggtt gaaaaacggg
    19381 cagacctttt tcctcagcca gaactttaac cgggctgggc atcagttttt taccgcgtcc
    19441 tgccggtcgg tctggctggg tgaacacgcc aacgacgtta tgaccagaag acaacagcgc
    19501 gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa ataatacgta gtgattctga
    19561 cacgttagtt cttatcctta agcccgggct ttcagacgat ctagtttttc aactttctga
    19621 cgaatacgtt gttgtttcag cggtgacaga taatccataa acagtttgcc gaccaggtga
    19681 tccatctcat gctgaataca gatggctaac agcccctctg cttccagttc aaatggttta
    19741 ccgtcgcggt caagggcgcg aattttaact ttctctgcgc gcggcactaa agcacgttgt
    19801 tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc cgcttttttc taaaagctcc
    19861 ggattgatta acaccagccg ttcgtcacgg ttttccgaaa catcaataac aatgatacgt
    19921 tgatggatat caacctgggt tgccgccagg ccaatacctt cttctgcgta catcgtctcg
    19981 aacatatcat cgacgatacg ctgtatttct gcattcactt cttctaccgg tttagcaact
    20041 ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa ctgacataaa tctccagaga
    20101 tgtgttcagg ggttagaaag attatttctt ctattctaga caaatccccc tctgattgac
    20161 agcatcactg accaatcgca aagattgcta aggctgctta tggcagggag ataaggatgg
    20221 tcgatacaga aatttggctg cgtttaatga gtatcagcag cttgtacggc gatgatatgg
    20281 tccgtatagc tcactggctg gcaaaacagt cgcaaattga tgcggttgga ttgcagcaaa
    20341 cagggcttac attgcggcag gcacaacgct ttctttcatt tccgcgaaag agtatcgaaa
    20401 gctcactttg ttggttggag caacccaacc atcatttaat ccctgcggac agcgaatttt
    20461 atcctcctca acttctggtg acgacagatt accccggcgc actgtttgtt gaaggagaac
    20521 tgcacgcgct gcattcattt cagcttgccg tagtggggag tcgggcgcat tcatggtatg
    20581 gcgagcgatg gggacggtta ttttgcgaaa ctctggcgac gcgtggagtg acaattacga
    20641 gtggactggc gcgtggaatc gatggtgtgg cgcataaagc tgccttacag gtaaatggcg
    20701 tcagcattgc tgtattgggg aatggactta ataccattca tccccgccgc catgcccgac
    20761 tggctgccag tctgcttgaa catggcggag ctctcgtctc ggaatttccc ctcgatgttc
    20821 caccccttgc ttacaatttc ccacgaagaa atcgcattat cagtggtcta agtaaaggtg
    20881 tactggtggt ggaagcggct ttgcgcagtg gttcgctggt gacagcacgt tgtgcgcttg
    20941 agcagaggcg tgaagttttt gccttgccag gtccaatagg gaatccggga agcgaagggc
    21001 ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga accggaagaa attctggaaa
    21061 acttgcaatt tggattgcac tggttgccag acgcccctga aaattcattt tattcaccag
    21121 atcagcaaga cgtggcattg ccatttcctg agctcctggc taacgtagga gatgaggtaa
    21181 cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt gccagaggta gttactcaac
    21241 tactcgaact ggagttagca ggatggatcg cagctgtacc cggcggctat gtccgattga
    21301 ggagggcatg ccatgttcga cgtactaatg tatttgtttg aaacctatat tcacactgaa
    21361 gctgagttgc gtgtggatca agacaaactt gaacaggatc ttaccgacgc aggttttgag
    21421 cgagaagata tctacaatgc cctgctatgg ctggaaaaac ttgctgatta tcaggaaggg
    21481 ttggcagaac cgatgcaact ggcctcggat cctctctcca tgcgtattta tacaccggaa
    21541 gagtgtgaac gactggatgc cagctgccgt gggtttctgc ttttccttga gcagattcag
    21601 gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag tgctcgcgct ggataacgca
    21661 gagttcgaac tggatgatct gaaatgggtg atcctgatgg tgttgttcaa catcccgggg
    21721 tgcgaaaatg cgtaccagca aatggaagaa ttactctttg aagtgaatga aggtatgctg
    21781 cattaatcat ttctttaatt cagcataagt tgttatggca aaatcagcac tgttcacggt
    21841 gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg gttattcgat ccgggaaaca
    21901 cggtccgttt cttggatgct cacagtatcc ggcgtgtgac tacgtccgtc ttctgaaatc
    21961 ttcagcggat ggacatattg tcaaagttct ggaggggcag gtttgccctg catgtggcgc
    22021 aaatctggta ttacgccagg gacgctttgg tatgtttatt ggttgcagta actaccctga
    22081 atgcgaacat accgaactta tcgataaacc ggacgaaaca gcaattacat gcccccaatg
    22141 tcggacgggc catctggtcc agcgccgctc ccgttatggc aaaacatttc actcctgtga
    22201 tcgctacccg gagtgtcaat ttgccattaa cttcaaacca atagctggag aatgccctga
    22261 gtgtcattat ccgctactca tcgaaaagaa aaccgcgcag ggtgtaaaac acttttgtgc
    22321 cagtaaacaa tgtggaaagc cggtttcggc ggaataataa cgtgaataat aacctgcaag
    22381 gagacgctat cgcagctgcg atagatgtcc tcaatgaaga acgtgtcatc gcctatccaa
    22441 cggaagccgt tttcggtgtt gggtgcgatc ctgatagcga aacagcagtg atgagactgt
    22501 tggagttaaa acagcgtccg gttgataagg ggctgatttt aatcgcggca aattacgagc
    22561 agcttaaacc ctatattgat gacaccatgc tgactgatgc gcagcgtgaa accatttttt
    22621 cccgctggcc aggtcctgtc acctttgtct ttcccgcgcc tgcgacaaca ccgcgctggt
    22681 tgacgggccg ctttgattcg cttgctgtac gagtcaccga ccatccgttg gtggttgctt
    22741 tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc caacttgagt ggattgccac
    22801 cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc ggcgttcccg gttgtgcctg
    22861 gtgaaacggg ggggcgttta aatccttcag aaatccgcga tgccctgacg ggtgaactgt
    22921 ttcgacaggg gtaacataat ggaaacctat gctgtttttg gtaatccgat agcccacagc
    22981 aaatcgccat tcattcatca gcaatttgct cagcaactga atattgaaca tccctatggg
    23041 cgcgtgttgg cacccatcaa tgatttcatc aacacactga acgctttctt tcgtgctggt
    23101 ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg cttttgccag agcggatgag
    23161 cttactgaac gggcagcgtt ggctggtgca gttaataccc tcaagcggtt agaagatgga
    23221 cgcctgctgg gtgacaatac cgatggtgta ggcttgttaa gcgatctgga acgtctgtct
    23281 tttatccgcc ctggtttacg tattctgctt atcggcgctg gtggagcatc tcgcggcgta
    23341 ctactgccac ttctttccct agactgtgcg gtgacaatca ctaatcggac ggtatcccgc
    23401 gcggaagagt tggctaaatt gtttgcgcac actggcagta ttcaggcgtt gggtatggac
    23461 gaactggaag gtcatgagtt tgatctcatt attaatgcaa catccagtgg catcagtggt
    23521 gatattccgg cgatcccgtc atcgctcatt catccaggca tttattgcta tgacatgttc
    23581 tatcagaaag gaaaaactcc ttttctggca tggtgtgagc agcgaggctc aaagcgtaat
    23641 gctgatggtt taggaatgct ggtggcacag gcggctcatg ccttccttct ttggcatggc
    23701 gttctgcctg atgtagaacc ggtcatcaaa ctattgcaac aggaattgtc agcgtgaatc
    23761 aggccatcca gtttccggat agggaagagt gggacgagaa taaaagatgt gtatgttttc
    23821 ctgctctcgt gaatggtatg caactgacat gtgcgatctc tggcgagagt ctggcgtatc
    23881 gctttactgg aggtacgcca gaacagtggt tagcgagttt tcgtcagcat cgctgggacc
    23941 tggaagaaga agcggaaaac ttaattcagg aacaaagtga agatgatcaa ggttgggtct
    24001 ggttaccttg atccagatat tcgtccttcc atttcacgta gttatttgcg gaatagcgta
    24061 acccagcctt ctcttcatca cttaacgggc ggatctgttt gacgggacta ccgagataca
    24121 gatatccgct ctccagccgt ttattttgtg ggaccaggct acccgcacca atcatcacat
    24181 catcttctac tattgcgcca tcaagtaaaa ttgagcccat cccaaccaaa actcgattgc
    24241 caatggtgca gccgtggagc atcaccttgt gaccaacagt gacatcttcg ccaatggtta
    24301 atgggttgcc atctgggttg tacgaggatt tatgagtgac atgcaacata ctgccatcct
    24361 ggatattggt gcgtgctccg atctgtacat aatgtacatc tccacgaatc acaacgagcg
    24421 gccagatccc cacatcatca gccagacgaa cgtcaccaat cacgacgctg ctatcgtcga
    24481 tcattacgcg ctggccgatt tgtggaaaaa gatcgcggta tgggcgtaaa acatcagaca
    24541 tactcacctc aacaataaat gatttcctaa tgactttggg tgcattatta gccttgtgca
    24601 agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgaa tgcagcaaaa agggtgaaaa
    24661 aacaacaaac agaaaaaaag atcaaaaaag tacttgtgca aaaaattggg atccctataa
    24721 tgcgcctccg ttgagacgac aacgtgaaac acttcacagg atggtcggaa caacgaagag
    24781 aaaaaattct gaaattcagg gttgactctg aaagaggaaa gcgtaatata cgccacctcg
    24841 cgacagtgag ctgaaagccg cgtcgcaact gctctttaac aatttatcag acaatctgtg
    24901 tgggcactcg aagatacgga ttcttaacgt cgcaagacga aaaatgaata ccaagtctca
    24961 agagtgaaca cgtaattcat tacgaagttt aattctttga gcatcaaact tttaaattga
    25021 agagtttgat catggctcag attgaacgct ggcggcaggc ctaacacatg caagtcgaac
    25081 ggtaacagga agcagcttgc tgcttcgctg acgagtggcg gacgggtgag taatgtctgg
    25141 gaaactgcct gatggagggg gataactact ggaaacggta gctaataccg cataatgtcg
    25201 caagaccaaa gagggggacc ttcgggcctc ttgccatcgg atgtgcccag atgggattag
    25261 cttgttggtg gggtaacggc tcacc
//
LOCUS       DAZRUF010000035        25229 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZRUF010000035 DAZRUF010000000
VERSION     DAZRUF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25229
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.035"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..238
                     /locus_tag="ACHXOL_001164"
     CDS             <1..238
                     /locus_tag="ACHXOL_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808193.1"
                     /translation="RAITFGNAFNVDLWEYVMDREYICASDPKAREMWQRIWRDYRLM
                     AKGWARCCYSSLVLKAVQLSLRHIPASLREPLLY"
     gene            251..538
                     /locus_tag="ACHXOL_001165"
     CDS             251..538
                     /locus_tag="ACHXOL_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808194.1"
                     /translation="MTCNRKRWSREDREFIEASVGKMTVEEMAKKLKVAPTALQAHAR
                     RHGISLCAYRVSEHDKYLCRELYKEGLAIHVIAKKMELSNRAVSGIVYSEY"
     gene            557..1081
                     /locus_tag="ACHXOL_001166"
     CDS             557..1081
                     /locus_tag="ACHXOL_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR6808195.1"
                     /translation="MAKPAKRIRNAAAAYVPQSRDAVVCDIRRIGDLQREAARLETEM
                     NDAIAEITEKYASQIAPLKTSIETLSKGVQGWCEANRDELTNGGKVKTANLVTGDVSW
                     RQRPPSVSIRGVDAVMETLERLGLQRFIRTKQEINKEAILLEPKAVAGVAGITVKSGI
                     EDFSIIPFEQEAGI"
     gene            1179..1727
                     /locus_tag="ACHXOL_001167"
     CDS             1179..1727
                     /locus_tag="ACHXOL_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HFR6808196.1"
                     /translation="MKAYAYFKLSDAAANGYTIRWLKDVSVKRDKIICSLQRAFGADG
                     VSLVSNRGYERVECIWMNQLAPELWRGKSDGLFIGGFQFYGVVPDITTPEGRNNATLI
                     QEHEEQLRKYPTFPVWLCNELGVDVVPNMFDLKSVWTMHHSRDGFGILFEVCLLDGEV
                     KGPVPAECQRIKHSEFVALTEE"
     gene            1731..2270
                     /locus_tag="ACHXOL_001168"
     CDS             1731..2270
                     /locus_tag="ACHXOL_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808197.1"
                     /translation="MIDAKVLNGVSTLLRAYGRLTCGVLAEKMNMLPSSMVYFLRDAV
                     DAGVLTECNGFYDVPRPRLTSPVRRNTTELPGVDDAAWCTFRRSLPWLEGNAIPALAK
                     EFATGVLTCESVYIVAEVDDEMCKQGMPRFVMAYIDIRLGRFICGSSGWNITDHVLRY
                     LILDYSPVPAETQEISGHD"
     gene            2263..2445
                     /locus_tag="ACHXOL_001169"
     CDS             2263..2445
                     /locus_tag="ACHXOL_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808198.1"
                     /translation="MIKSIGFVLLVGTCGNDACDAIPVTEKIWPTEQACMQVMERIQK
                     RYPGEIFYCEDALRNE"
     gene            2438..2800
                     /locus_tag="ACHXOL_001170"
     CDS             2438..2800
                     /locus_tag="ACHXOL_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808199.1"
                     /translation="MSKRIYDDPKYRAEMRAQLVVSGYTPEQADATLTELMGERPFDE
                     AEYEYWLKQFRLEILENTTLPSLIRIIKSVIDNACYNGDCLAAAIATHALVNLLEVTG
                     AVFLNPEKVLRDENTDDY"
     gene            2778..3113
                     /locus_tag="ACHXOL_001171"
     CDS             2778..3113
                     /locus_tag="ACHXOL_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808200.1"
                     /translation="MKIQMITDADLMAATLISSGMSEQKVNDFLDNVVYQRYEQAREN
                     LISEKRTMIAESLSPAWIIEELRKIIASQDKVAAVAALDVLHRMSKELFNTELILNPG
                     GHNEPHIPD"
     gene            3091..3642
                     /locus_tag="ACHXOL_001172"
     CDS             3091..3642
                     /locus_tag="ACHXOL_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFR6808201.1"
                     /translation="MSRTSLIKLIHVARRDLQLDDDTYRTFLVQCTGKTSCRELSVAQ
                     LERVLDAMKERGFKKQKKHPRRRFKGHVTPREKIYKIWQQMFLDGFVSDISDAALDKY
                     VERLTTRRNGGQGVSTLAWCHGESLQVVLETLKQWHMRCIREAFARHGVPLPVSKTGQ
                     ELRGYDALTSAYARARNSGRIAV"
     gene            3639..4088
                     /locus_tag="ACHXOL_001173"
     CDS             3639..4088
                     /locus_tag="ACHXOL_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFR6808202.1"
                     /translation="MKQMKEQDLFEDFRDDSVLEYLNDYQENTAYPALLSELNALLRK
                     ELARIGADPAHSLELVVAICRHIGGMQVYVPRGNILENLVRDMRIWRDFNGHNIPELV
                     QRYGVTYKTVYKAIKRMRRLECNKYQPDLFYHSPNLPHPVRRPPRRP"
     gene            4174..4761
                     /locus_tag="ACHXOL_001174"
     CDS             4174..4761
                     /locus_tag="ACHXOL_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HFR6808203.1"
                     /translation="MENLKFSPAFEHALNFILRPDIEGVYVNDPTDRGGETRYGISDR
                     RDGVIDGKTDVSGDGKPDTRIRDLTREQVAQIYWRDYWLPAGCDQWPDGVAMFVFDAA
                     VQHGVKKAIRILQEAADVDADGIIGPRTRKAVSLSTPDWLLTRCIVRRSRFYADIIKS
                     KPAQGKYLNGWFNRMEKVTDACLEIIDNVSSVTRG"
     gene            4763..4990
                     /locus_tag="ACHXOL_001175"
     CDS             4763..4990
                     /locus_tag="ACHXOL_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808204.1"
                     /translation="MGKGWDASLRAGRRDRLRQEVLHRMAGGPPPKPLDYTGHDGTHA
                     SHYMRGWNTVDTRDIFWQCQKYKEKLNVQNE"
     gene            4974..5384
                     /locus_tag="ACHXOL_001176"
     CDS             4974..5384
                     /locus_tag="ACHXOL_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005110321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808205.1"
                     /translation="MYRMNSLFVRVIYSRFMLCQWSFLGLSAVLFLASCFAGWQAFAV
                     MVLAYGATLCLIHSFQLGNVPYRLQPKRRFRAASLRIVVWSWGVWAVGFFMLTFGLLY
                     LKEPYQLAFWLGGIFCAALYKHQRRFHKGDSWTR"
     gene            5372..5980
                     /locus_tag="ACHXOL_001177"
     CDS             5372..5980
                     /locus_tag="ACHXOL_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808206.1"
                     /translation="MDPLTLSGIASVLLKAGPGLIRSVGRWFGGSTSAAADSVAGMVE
                     SVRESLPVAEQQRILEQKLVMLSPEQLVQLETLKVQLQQLEVERQKLVLADQQAAHHE
                     QQETIRNGDNATDSYVRQTRPLLARLSCYSSLAYVLLLSCGQIAGAVAGANGITLHMP
                     SPDWDITLMLFTPALGYLGVRTLDGFARYSKSSCHKISAGPK"
     gene            5977..6204
                     /locus_tag="ACHXOL_001178"
     CDS             5977..6204
                     /locus_tag="ACHXOL_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFR6808207.1"
                     /translation="MTDETDRISEVVLNERQDVVNAWLMRIKEAPHSRGFCNDCGNVI
                     PAQRIAVLPDVVTCIDCQQARERRRKTCPGK"
     gene            6189..6494
                     /locus_tag="ACHXOL_001179"
     CDS             6189..6494
                     /locus_tag="ACHXOL_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFR6808208.1"
                     /translation="MSWEVIRANWPVLWALLMTAINLIQLILAKTYVKREEFDSLRSR
                     VSVMEGLVNQLPDRNEFHRLQLDISNLRGEIKELGPSIRQVSRISDLLLENELKEKN"
     gene            6500..6787
                     /locus_tag="ACHXOL_001180"
     CDS             6500..6787
                     /locus_tag="ACHXOL_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005103431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFR6808209.1"
                     /translation="MKEILTEDRRLVLLRSLLDCGDSANESVLQTCLQAYGHKVSRDV
                     VRTQLAWLREQGLVRLSDVGGCYVAEITGSGEDVANGLSGVPGVKKPRARD"
     gene            6790..7077
                     /locus_tag="ACHXOL_001181"
     CDS             6790..7077
                     /locus_tag="ACHXOL_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808210.1"
                     /translation="MARKLKPLSRGERAVVRQLAYCLVLADIEQNAIVRAYEKHTGKP
                     WNPDAPDTPVKRALRSSPAYARLWKLPGKDIQSVREEIYAGLKTQRTEDGK"
     gene            7067..7648
                     /locus_tag="ACHXOL_001182"
     CDS             7067..7648
                     /locus_tag="ACHXOL_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HFR6808211.1"
                     /translation="MENEQRPTRGRLSKVDLLPDSIREQLHQMLREKRHTQEEIREAI
                     NALIDEHNLPGEMQLSRTGLNRYASRMEKVGAKIRASREMAEVWAAKLGSAPTSDVGR
                     LLMEFVKTLAFETSMSMTEDDKPVAPKALGQLALVAQRLEAAAMTSHKREKAIRDAFA
                     QEMAEKTEELVRAGGLSGGAADTIKREILGISV"
     gene            7645..9180
                     /locus_tag="ACHXOL_001183"
     CDS             7645..9180
                     /locus_tag="ACHXOL_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR6808212.1"
                     /translation="MTQMNAFSESDVLLPYQKRWVADDSKLKIAEKSRRTGLTWAEAA
                     DAALTASLKKENGGRDHFYIGSNKEMAREFIDAVAMWAKAFNAAAEEIREEVITDEDK
                     DILTFVVYFASGFKVKALSSNPGNIRGMQGNVTIDEAAFHEKLDEVLKAVTPLTTWGG
                     KVRLISTHDGVDNPFNQLIQESRAGKKDYRIHTITLDDACNDGLYRRICQVRGMVWSP
                     EAEVEWKEGLLRNTATREDALEEYYCVPKNGGGTYIPRSLRERAARGTGKVLRFTGTP
                     EFNALTEGQRRAEIREWLETVVRPELEKLPKNLRHCLGEDFARSGDLTVLAPVTVNDD
                     TTREVPFLVELANVPFKQQEQVLFWLCDRLPRRDGIKMDARGNGQYLAEQAAERYGDE
                     VEQVMLSVAFYRENMPRFRAAFEDDELILPKHEDVINDLGAIQLLRGVPGIDDARTKG
                     SDGRKRHGDAAVAIFLGFLASKDDCHRYELHRLNRPAKPEEGNARRQMKLTRGLKNEG
                     GLL"
     gene            9177..10748
                     /locus_tag="ACHXOL_001184"
     CDS             9177..10748
                     /locus_tag="ACHXOL_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFR6808213.1"
                     /translation="MNLKQLAGAVRRLLNPATGEEDTLKKEALNEAQARPRHAGVRPT
                     SPGISVASGLNPGRLAGILRNAADGITRDFFILAEEMEERDLHYASVLRTRKLTVAGI
                     EPVVVAASDDDADVQLADAIRALMEAPQIPELMFDLLDGLGKGVAVCEILWDTRNNHW
                     MPRDYEWVDPRFLKAEKPTLRQFRLLTDDEPVDGVPLTPGKFIVHQPRLKSGLPLRNG
                     LARLVAVMYMLKSFTVRDWWAFAEKFGIPVTVGKYGPNATEEQIRVLIDAIASIASDA
                     GCAIPQSMQLEMQETASRNNGGALFREMAEWCDAQISKAVLGQTMTTDNGSSRSQADV
                     HNQVRMDIVRWDARQLANTLNEYLVRPYIEANYGPQAHYPRVVLRISEAEDLKALTDA
                     LVPLIDRGMRVQESELRDRFGLAEPDEGADVLHPVSAGMPGDMAMNRERVALNREHPD
                     ELAQMVDDALRDWQKTGEAFTNPVLTLAQECDSFDDFLKRLPELQETLNADDFALQLA
                     EVCFKARALGDTAHA"
     gene            10741..11505
                     /locus_tag="ACHXOL_001185"
     CDS             10741..11505
                     /locus_tag="ACHXOL_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR6808214.1"
                     /translation="MRETLIPKEALAWLKAKKLRPGFDYRDVWREEHRNSFTVAKMLQ
                     LDLLSDVKALVEDALQSGQTFSEFREMLQPLLIKRGWWGVQEMDDPLTGETRTVQLGS
                     DRRLRTIFDTNMRTARAAGQWERIQRTKQAMPYLMYELGPSREHRVEHVKWARLCLPV
                     DHPFWQTHFAPNGWGCKCTIRQVSRGEYAQLAAQGTIHTEAPEIRTVRWVNKRTGEEE
                     DVPEGIDPGWNYNPGINREQELARQLAARQARFNSE"
     gene            11720..12676
                     /locus_tag="ACHXOL_001186"
     CDS             11720..12676
                     /locus_tag="ACHXOL_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFR6808215.1"
                     /translation="MQPELLALCFSLPEPIPELTPAQLPEWLELVPAGEFTGRDGRTW
                     MNRNPHEVVARSSDINIPVDIEHATEIKGPRGEEAPAYGWVEALRVTDSGTIEGRVVW
                     SESARWMLSERRYRYYSPAFFHDADGAVTRLSSVGLTNKPNLDFPALNTEKNPMTVPV
                     QITGLLGLAESATVDDTVAAIKQLQENEQVALNRAQTPDLTKFVPVETHNLALNRAET
                     AEKRLQQLEEKEAEALVDAAIGAGKVAPANRDMFLATCRTEEGRKQFAEYTKGAQPLV
                     NNDKPSQGKDKPAQTLTDAELAMCRSMGITGEEFLAAKPKQE"
     gene            12684..13589
                     /locus_tag="ACHXOL_001187"
     CDS             12684..13589
                     /locus_tag="ACHXOL_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFR6808216.1"
                     /translation="MSQTISSEILHALTTCLSAAFTRGLSGVEPQWNRIASEVPSSSA
                     SNTYGWMKDLPDIKEWVGERQLATLDGYGYTITNKLWESSIRVKREHIEDDQIGQYSI
                     TAERYGRMTAVFPDKLCYALLCAGFNTLCFDGQNFFDEDHPLGDGTYSNVVGTPASDK
                     GEPWFLIDESQVLKPIIWQTRRAFKFEALDDLNSEHTFKNHEFLYGVDGRCNAGFGFW
                     QTAVGSHAALTVENYKKANELLRGMKGTNGEPLGIRPTTLVVGPKNRADAKLIIDAML
                     VNGGDSNIWYKDVKIVDSPYITTPA"
     gene            13661..13972
                     /locus_tag="ACHXOL_001188"
     CDS             13661..13972
                     /locus_tag="ACHXOL_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808217.1"
                     /translation="MSEKAETKGAKAAKNSAAQENPAPLADVIVADGQANEPRPAEDP
                     VAVQGDAPVRLNVRAVSENGFWRCGRFWSHIGEDVAVSAAVATRLMAEPNLIVREAEK
                     G"
     gene            13972..14409
                     /locus_tag="ACHXOL_001189"
     CDS             13972..14409
                     /locus_tag="ACHXOL_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016807456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HFR6808218.1"
                     /translation="MGYITQEDLLRADGNLVWNMAINRETNGLDEDKIRQAISDAEAE
                     IDSFLSRRYQLPLGVTEIPRPLQRVAVSLAFYWLSERDNQITELIQKRYDDAIKTLRE
                     MANGTRDLGLPTYATPAETDNGKIIVVGANARLFTRNNLKGVL"
     gene            14409..14951
                     /locus_tag="ACHXOL_001190"
     CDS             14409..14951
                     /locus_tag="ACHXOL_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004114586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR6808219.1"
                     /translation="MGISVQVSGDQRLEDIRRAVEKLAEGSLQSELLESIGAVVESQT
                     RRRIIDEKTSPGGERWPDWSDGYKKTRHGNQSLLRGEGHLLESIQYIVENRVVRIGSP
                     LDYARIMNDGFSGSVPVSAHKRLISQCFGRALKYPVWQTVGAHHRMMNIPQREFLGLS
                     SASQQELQQVISHFWKEVLP"
     gene            14948..15559
                     /locus_tag="ACHXOL_001191"
     CDS             14948..15559
                     /locus_tag="ACHXOL_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808220.1"
                     /translation="MTEQRPELRTPGSTVAAAERIVAWLQTALQGDTPDRADVVERHI
                     GQFNSTDEVKRYLSGRTGCIRVAALRVRDINPRGGLSGLVTWVAYIMATDSWGYSRDV
                     RCEVLAGKVIKRLLSSDATGGMGAERMAADVRADNIYSVSLDGLGVTMWAVTWEQEFR
                     LDEEIDLAALPDFLRLGATLRSGEHTEINDVIHVRGDDGTETD"
     gene            15540..15725
                     /locus_tag="ACHXOL_001192"
     CDS             15540..15725
                     /locus_tag="ACHXOL_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFR6808221.1"
                     /translation="MEQKLIKPARENVRVRKPDGAHLSPEGERLNVCAYWLRREAEGD
                     VEITTLPKNNNKTRGKK"
     gene            15725..17200
                     /locus_tag="ACHXOL_001193"
     CDS             15725..17200
                     /locus_tag="ACHXOL_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR6808222.1"
                     /translation="MSLGSIPDDIRVPLVWIDIDNSQALEGASAQSRKILVMGHAVSS
                     GSADALSLTRITSDSQADQLYGKGSMLAEMLKMLRRANTYTETWAMPVAAPEGAAAKA
                     TLTVLGTATEAGTVALLINGVSVQVSVSAGATKENIAKAIADAVTKKPATQVAAAVKD
                     DATDTVELTVNWHGVTGNGADVRLNYHTGEAFPAGVKVTATAFTGGTGTPEMADAVAA
                     IGPEWFTDIIAPFTDTKSLNTLRDELLNRWGPLKMMEAQLWTAFRGTHGETGTFGETR
                     NDWLISCIGTNLSPHPAWMWAASYGATAAYHLAIDPARPLQTLVLTGILPPARNVRWD
                     MPERNLLLHDGIATHMVDAGDNVCIEREITMYRVNQYGDADVSYLDVQSPATLGRIRY
                     IIKNRFTNRYPRHKLADDDVLDSLDAGQPVMTPKLCTAELLDICQTELLPAGLVENFS
                     DYKDTLQVTRDSSDKNRLNFICHPNLVNQLRVLAGLIQFKL"
     gene            17210..17566
                     /locus_tag="ACHXOL_001194"
     CDS             17210..17566
                     /locus_tag="ACHXOL_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR6808223.1"
                     /translation="MAKILGMATIRVNGREIKTEGKSTLNPGGFSRTQHMGGGKVWGI
                     SSKMASPSIKVTIAAAADMDVIEISSWEDVTVMFYGNNGLNYMMTGSATDNPAELDED
                     SGTISANFIGEKCVKV"
     gene            17568..17963
                     /locus_tag="ACHXOL_001195"
     CDS             17568..17963
                     /locus_tag="ACHXOL_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR6808224.1"
                     /translation="MAAMEFELKHGLLTGKGTADETLHKTVKLRELTASDVIDAQLAA
                     ERVVMGGNGKAVAYCSEVLMGLEMMRRQVASIGNIPGPLDMKQLRMLHPADLELISTK
                     AAALDEMLEEVATRGRTDAAGGGSDEPAG"
     gene            18050..19843
                     /locus_tag="ACHXOL_001196"
     CDS             18050..19843
                     /locus_tag="ACHXOL_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808225.1"
                     /translation="MATGNRLSTEIMINLAGNLTAKARQYGANMSQFARNNQKAMRLV
                     KATTEAAGRGLDTLGNRYTAMIAGLGSSLTIKQVADFDAQMRRMGTDAQLTTEQVKTL
                     HDKIRDVSNQKDIRIDASALGQGASELLGKTGDYQYVVDNLRNMGLMMQAFGVDGQVA
                     AGLMAQFWEKNVRGADAVNNMMDRLYSQFAVGSVSVADVARAAPKLFSIIQDQGPEAI
                     AQMGAFAQVFAKNKGSIDETVTSIQAMYASLSDKKNIEFLKKNGIDVFVKGTKDIKKP
                     YELMMEILKRAKYDPLKLQDVFDLTGMQGIKALLNPENRQLMEQMIYGTIELGSTQKA
                     AQTNAEGFNAAIQSLNNEWQRFAEGQLAKPVQDLADALNSVDQNTAQNWLQVGKYMAI
                     ALGGIIAIRKTYQFGKTIHDIMNPKGKGKGIPGSITDVFGSGVMPVYVVNMGSGGMKG
                     NTGGLPDAPDSSRNPRNSRNPRGPKTPALVTPQTASGFNLLDMVLSAFPKNKEEADAL
                     IARVQENNNRPTVWTDIRNWFNSLEKKNIADPRPWAGMQPTQNYPFLPQQLQGEIRVV
                     VEGDARVKSVRVDQPGVRLSAQAGVTSVEQG"
     gene            19843..21117
                     /locus_tag="ACHXOL_001197"
     CDS             19843..21117
                     /locus_tag="ACHXOL_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR6808226.1"
                     /translation="MTTSNGKWDGLRDASFRGVPFFLVDTEGTGGRRAIPRAYPRRET
                     AWTDDNGAVPGQQQINAKLPGKNFRDDLNALLDALNTPGPGELVHPWFGIQTVQVGKV
                     THRLSTEEDGIAYVTFEVFEAGERLFPSAADNTQQEVLTGIDAVKAAIDDGDWFGALD
                     GLGDMADSFLADMENLVANLPTLPAALNQWMDRLKHFKAMAGTLIATPGRLVSELSAR
                     VEDIAGLVTEPPEALAVYTTLRNQWAGERARQIATGALPEDITVKPGSVADGNPGFAI
                     GLSPDYQPVSDSLQKNIDDFRQVVVLGALLGQASAVASMTFDTRDAALSAGDTLAAEL
                     HEQAVAAVENNQRTLWRTLRDLRQAVITDARERAARLPETRQVTLTTTTSAALLAWRE
                     HGDTRRRDEIVQRNRLRHPSFILPTQPVEITD"
     gene            21117..22187
                     /locus_tag="ACHXOL_001198"
     CDS             21117..22187
                     /locus_tag="ACHXOL_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR6808227.1"
                     /translation="MESVILTVDGKLWEGWTEMSVSRSLKAIAGEFELSVTTRWSAAA
                     PRVIREGQPCTVRLGADTVLTGYIDDFIPGYDAENVEIRVMGRDKTGDLVDCSVVHSS
                     GKWKGVRLEQVAADVCRPFGITVITETPTGEAFASVVLEQGETGFELLDRLAKQRGVL
                     LTSDGAGNLIITRASSVRAGVSLVLGKNILAARGRFSWRERNSQYIIKGTTSAGGKLW
                     DSQPAPMVGGRQYITEDPEINRYRPRILVNEDSLTVGGASIRGEWYKARMLGESRTTE
                     ITVAGWREQGDSGPLWQTNRLVDIDDSIQNLKTTWLISGVTWTDGAQGRMTVLALVPP
                     ESLDMPEMKAKTKKTKAVATWD"
     gene            22178..22714
                     /locus_tag="ACHXOL_001199"
     CDS             22178..22714
                     /locus_tag="ACHXOL_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR6808228.1"
                     /translation="MGLNAIARRLRLMVDRAVVRLVSDGLARQNLQIQTLADATDDDV
                     ERFQNYGFTSVPPEGSEAIVLAVGGRRAGLVAIAVEDKRCRPKGLSPGDVRVYHRDGK
                     SHITLKENGVIEITGEQVDVSGKTVNLTADELLNIIGKQMKFVGPCEFTEDVRIKGKS
                     FSEHIHKDGDNEKTSPPV"
     gene            22711..23163
                     /locus_tag="ACHXOL_001200"
     CDS             22711..23163
                     /locus_tag="ACHXOL_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR6808229.1"
                     /translation="MTTEIRWNNMLSRGDITVTHNGLSRDEGLVTQVLICLFTDARAD
                     DDDVIPDGSGDPRGWPGDTYSDFSWGSRLWLLEREKLTEDVRLRVEDYARLSMQPLLR
                     AGYARNATVTASIIIPDRIAFLVVLTRPDKTTLRIEITRRWEATINAV"
     gene            23153..24229
                     /locus_tag="ACHXOL_001201"
     CDS             23153..24229
                     /locus_tag="ACHXOL_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR6808230.1"
                     /translation="MPYEIPTLGKLIADGEKDIAYELGLQKLPPVSVEQALNVSFSSQ
                     VRDLYDHQSWIKDQIIPSTTSDDETIIKTAKYEGVIRKQATFASGRVTFTSQSPLPAE
                     TRMQSDTNQVYQVLTSGEVQDGEVTVIVQAEEAGVAGNLAAGAVLTLLSPLPGTASTG
                     AVAEGGITGGADTESIAALLDRLLYVRRNPPTGGALHDYVIWAREVPGVSRAWAWDAW
                     HGPGTVGLAWLYDDRENIVPTREDLKTMEQYLFCHKHPATGVMVGKPGGIEVWPVQVR
                     LKKVSISIRLTPDSLANRSAVRANLTALQKTLAPGQTLPVSSLRTAIGMTSGITDYFL
                     NIGEDTTSDVDELITIGEVTWLTA"
     gene            24214..24798
                     /locus_tag="ACHXOL_001202"
     CDS             24214..24798
                     /locus_tag="ACHXOL_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR6808231.1"
                     /translation="MAHSVTEWLAALQQVMPRGKAWPRDNDADLNRFLRALAERLNRT
                     EYDASRLHVEMRPETTLRLLPEWEQYLALPECGIAPTTREARRRAVVEKYRRRGGLAT
                     WQTEAAAAALGFTIKVTAVLPHHCLRDCMYPLHPARYRWMLKVNVPGKDAGRFTCIDG
                     VMTPLISERTRELECLLKHYRLAGTEYEYYYTGE"
     gene            24802..>25229
                     /locus_tag="ACHXOL_001203"
     CDS             24802..>25229
                     /locus_tag="ACHXOL_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808232.1"
                     /translation="MFYVDNPTGVPVMPPVAAELSKTTLYFTEGGNGIPPTYPGPDWF
                     NIIQSELLEILRQANIKPDKNTTDQIMTALKKLFITNSGSAGAIAGLTGQNNTFPYFT
                     GKDTMALTPLSAFVRGILGKESATDFADALKVIKQSGGTMT"
BASE COUNT         5918 a         6629 c         7557 g         5125 t
ORIGIN      
        1 ccgcgccatt acgtttggca atgccttcaa tgtggattta tgggaatacg tcatggaccg
       61 cgaatacatc tgcgcgtcag acccgaaagc ccgtgaaatg tggcagcgta tctggcgcga
      121 ttaccggtta atggcaaaag gctgggcgcg ctgctgttat tcctcgcttg tcctgaaagc
      181 ggttcagtta tcgctgcggc atattccggc atcactgcgc gagcctctgt tgtactgagt
      241 ggtgaccgga atgacgtgta atcgtaaacg ctggtcacgc gaagaccggg aatttatcga
      301 agccagtgtc ggaaaaatga ccgtcgagga aatggcgaaa aaactgaaag ttgccccaac
      361 cgccctacag gcacatgcca gaaggcacgg aatatcgttg tgtgcatacc gggtcagtga
      421 gcacgacaaa tatttatgcc gtgaacttta taaagaaggg ctggctattc atgtgatagc
      481 caaaaagatg gaattaagca atcgtgctgt atccggcatt gtatacagtg aatattaatt
      541 aacaggagct ttatttatgg ctaaacccgc aaaacgaatc aggaacgccg cagcggctta
      601 tgttccgcaa tcccgcgatg ccgtggtatg tgatatccgc cggattgggg acctgcaacg
      661 cgaagcagca cgacttgaaa cggaaatgaa tgacgctatc gctgagatta cggagaaata
      721 cgcctcacag attgcaccgc ttaaaaccag tatcgaaacc ctttcaaaag gcgttcaggg
      781 atggtgtgaa gcgaaccgtg acgaactgac gaacggcggc aaggtgaaga cggcaaacct
      841 ggtaaccggt gatgtgtcat ggcgtcagcg cccaccatca gtaagtattc gcggtgtgga
      901 tgcagtgatg gaaacactgg agcgtcttgg ccttcaacgc tttattcgca cgaaacagga
      961 aatcaacaag gaagcgattt tactggagcc gaaagcagtc gcaggtgtcg ccggaattac
     1021 agttaaatca ggcattgaag atttttctat tattccgttt gaacaggaag ccggtattta
     1081 ataccacgac gaatatttaa ttaattcact ttcttttaat tatggcgcga tgcgtcaggg
     1141 gattgctcgc gcctgaatca ggaccagagg aaatgaatat gaaagcatat gcttatttta
     1201 aactgagtga cgcagccgct aacggttata cgattcgctg gctgaaagat gtttctgtta
     1261 agcgtgacaa aatcatctgc tcattgcagc gtgcgtttgg cgcagacggt gtttcccttg
     1321 tcagtaacag agggtatgaa cgggttgaat gtatctggat gaatcaatta gcgccggaat
     1381 tatggcgtgg taaatcagac ggactgttta ttggtggatt tcagttttat ggcgttgtac
     1441 cggatataac aacaccggaa ggccgaaaca atgcgacgct tattcaggaa catgaagagc
     1501 aactccggaa atatcccaca ttcccggtct ggttgtgtaa tgaactcggg gttgatgttg
     1561 tacccaacat gtttgatctc aaatcagtct ggacgatgca ccacagccgt gacggattcg
     1621 gcattttgtt tgaggtgtgc ctgctggatg gtgaggttaa gggaccggtt cctgccgaat
     1681 gccagcgcat taaacattct gaatttgtgg cactgacgga ggaataaccg gtgattgatg
     1741 caaaagttct gaacggtgtc agcacattat tacgggctta cgggcgtctg acctgcgggg
     1801 ttctggctga aaaaatgaat atgctgccct cgtcaatggt gtatttcctg cgtgatgcgg
     1861 ttgatgccgg agtgctcacc gaatgcaacg gattttatga cgttccgcgc cctcgtctga
     1921 cgtcacctgt aaggcgaaac acaacggagc tgccaggggt tgatgatgct gcatggtgca
     1981 cgttccgtcg ttctttgcct tggctggaag gtaacgccat tccggcactg gcaaaagaat
     2041 ttgcgacagg cgtactgacc tgcgagtcgg tttacatcgt tgctgaagtg gatgacgaga
     2101 tgtgcaaaca gggaatgccc cgttttgtga tggcgtatat cgatatccgg ctgggtcgtt
     2161 ttatttgtgg ctccagtggc tggaatatca ccgaccacgt cctgcgctat ctcattcttg
     2221 attattcacc ggttccggcg gaaacacagg aaatcagcgg gcatgattaa gtcaattggc
     2281 tttgtcctgc tggttggcac ctgtgggaat gatgcctgcg atgccatacc tgtaacagaa
     2341 aaaatctggc ccacagagca ggcatgtatg caggtgatgg agcgcataca aaaacgttat
     2401 cccggtgaaa tcttttattg tgaggatgca ttaagaaatg agtaaacgta tttatgacga
     2461 cccgaaatat cgggctgaaa tgcgggcgca actggttgtc agtggatata cgccagagca
     2521 ggccgatgca acactgacgg aactgatggg tgagcgtcca tttgatgaag ctgaatatga
     2581 atactggctg aagcagttca ggctggaaat tctggaaaac accacactgc catcgctgat
     2641 aagaataata aaaagcgtta tcgacaatgc ctgctacaac ggtgattgcc tggctgcagc
     2701 aattgcgacc catgctctgg tgaatctgct ggaggttacc ggcgcagttt tcctgaatcc
     2761 agaaaaggta ttaagagatg aaaatacaga tgattactga tgctgattta atggctgcaa
     2821 ccttaatttc cagtggcatg tcagaacaaa aggtaaatga ttttctggat aatgtcgtat
     2881 accaacgcta tgagcaggcc agagaaaatt taatttcaga aaaacgcacg atgattgccg
     2941 aatcattaag tccagcctgg attattgagg aattacgaaa aatcattgcc agccaggata
     3001 aagtagcggc agttgccgcc ctcgatgtgc tacatcgaat gagtaaagag ctattcaaca
     3061 ccgaattaat tttaaatccg gggggacata atgagccgca catccctgat taaattaatt
     3121 catgttgccc gtcgtgacct gcaactcgac gatgacactt accgcacctt tctggtgcag
     3181 tgcacgggca aaaccagttg ccgcgagctt tccgttgcac aactggaacg tgtgctcgat
     3241 gccatgaaag agcgcggttt taagaagcag aaaaaacatc cccgccgtcg ctttaagggg
     3301 catgtcacac cgcgcgagaa gatttataaa atctggcagc agatgttcct tgatggtttc
     3361 gtctccgata tcagcgacgc tgcgctggac aaatacgttg aacgcctgac aaccagacgt
     3421 aatggcggtc agggtgtttc cacgctggcc tggtgtcacg gggaatcgtt gcaggttgtg
     3481 ctcgaaacgc tcaaacagtg gcatatgcgc tgcatccgtg aagcctttgc ccgacatggt
     3541 gtgcctttac ctgtgagcaa aaccgggcag gagctgcgcg gctatgatgc actgaccagt
     3601 gcgtatgccc gcgcacgaaa cagcgggaga attgctgtat gaaacagatg aaagagcagg
     3661 atttattcga agacttccgg gacgacagcg ttctggaata ccttaacgat taccaggaaa
     3721 acacggctta tccggcgtta ctttctgaac tgaacgcact gctgcgtaaa gagctggccc
     3781 ggattggcgc agaccctgct cattcccttg agctggttgt ggcgatttgt cgccatatcg
     3841 gcgggatgca ggtttatgtt ccgaggggga atattctgga gaatcttgtc cgggatatgc
     3901 gtatctggcg tgattttaac ggccataata ttcctgaact ggttcagcgt tacggggtga
     3961 cgtataaaac cgtctacaag gccatcaaac ggatgcggcg tctggagtgt aataaatacc
     4021 agccggattt gttttaccac tccccgaacc tgcctcaccc cgttcgtcgt ccgccacgac
     4081 gtccttaatc aatgaagccg gtaaatccgg ctttttttat gcctccggca ccatgaagca
     4141 gaccacgttt aactctgctt cacaggtgct tttatggaaa atctgaaatt ctccccggcc
     4201 tttgaacatg cgctgaattt tattctgcgt cccgatatcg aaggcgtcta tgtcaatgac
     4261 cccactgacc gtggcggcga aaccagatac ggcatttctg accgccgcga cggtgtgatt
     4321 gacggcaaaa ccgatgtcag cggcgacggt aaaccggata cccgcatcag ggatttaacc
     4381 cgcgaacagg tcgcgcagat ttactggcgc gattactggc tgcctgccgg atgtgaccag
     4441 tggcctgatg gcgtggcgat gtttgtgttc gatgcggcgg ttcagcatgg cgttaaaaaa
     4501 gctatcagga ttttgcagga agccgctgat gtggatgctg acggcatcat cggtccgcgt
     4561 acccgtaagg cagtgagcct gtccaccccg gactggttgc tgacccgctg cattgtccgt
     4621 cgttcccgct tttatgccga catcatcaaa tccaaacctg cccagggcaa atacctgaac
     4681 ggctggttta accgcatgga gaaagtgacc gacgcctgtc tggaaattat cgacaacgtg
     4741 tcgtccgtca cgcggggatg atatgggcaa aggctgggat gcctcgctga gagccgggcg
     4801 gcgtgaccgc ctgcgtcagg aggttttaca tcgtatggcc ggaggtccgc ccccgaaacc
     4861 gctggactat accggtcatg acggcacaca cgccagccac tacatgcgtg gctggaacac
     4921 cgtggataca cgggacattt tctggcagtg ccagaagtac aaggaaaaac tcaatgtaca
     4981 gaatgaatag tctttttgtt cgcgttattt actcccgctt tatgttgtgc cagtggtcgt
     5041 ttctggggct gtcggcagtc ctttttctgg cgagctgttt tgccggatgg caggcgtttg
     5101 cagtcatggt gctggcatac ggcgcgacac tctgcctgat tcacagtttc cagctgggca
     5161 acgtacctta tcgcctgcaa cccaaaaggc gtttccgggc cgcctccctt cgaattgtcg
     5221 tgtggtcatg gggggtctgg gccgttggtt ttttcatgct gacattcggc cttctttatc
     5281 tgaaggagcc ttatcagctg gcgttctggc tgggcggtat cttttgtgcg gcactttata
     5341 aacatcagcg ccgttttcat aagggggatt catggacccg ttaacccttt caggcatcgc
     5401 ctccgttctg ctgaaagccg ggccggggct gattcgttcc gtcgggcgct ggtttggtgg
     5461 cagcacatct gccgccgctg actcggtggc cggtatggtg gaaagcgtcc gggaaagcct
     5521 gccggtggct gaacagcaac gcattctgga acaaaaactg gtgatgctgt caccggagca
     5581 actggtgcag ctggaaaccc tgaaagtcca gctgcaacag ctggaggtgg agcggcagaa
     5641 gctggtgctg gctgaccagc aggccgctca ccacgaacaa caggaaacca tccgcaacgg
     5701 tgacaacgcc acggacagct atgtgcgcca gacgcgcccg ttgctggccc gtctgtcctg
     5761 ttacagcagt ctggcctatg tgctgttgct ctcctgtggc cagattgccg gtgcggttgc
     5821 cggtgcgaac ggtatcacgc tgcatatgcc gtcaccggac tgggatatca cgctgatgtt
     5881 gtttaccccg gcgctgggct atctcggggt aagaaccctt gacggctttg cccgttacag
     5941 caaatcgagc tgtcacaaaa tatcagcggg gccgaaatga ccgatgaaac cgaccgcatc
     6001 agtgaagtgg ttttaaacga acgtcaggac gtcgttaacg cctggctgat gcggataaaa
     6061 gaagcacccc acagtcgggg cttctgcaat gactgcggta acgttattcc ggcacagcgc
     6121 attgcggtgc tgccggatgt ggtgacctgt attgactgcc agcaggcgcg ggaacgcagg
     6181 aggaagacgt gtcctgggaa gtgatccggg ccaactggcc tgttttatgg gcgctgttga
     6241 tgacagcaat aaacctgatt cagctgattc tggcgaagac ttacgtcaaa cgcgaagagt
     6301 ttgattcgct gcgttcccgc gtgtcggtga tggaaggcct ggtgaatcag ctacccgacc
     6361 gcaatgaatt tcaccgcctg caactggata tcagcaacct gcggggtgaa atcaaagagc
     6421 tggggccatc catccgccag gtatcccgca tcagcgattt gttattagag aacgaattaa
     6481 aggaaaaaaa ttaatggcga tgaaagagat cctcactgaa gaccgtcgtc tggtgttgct
     6541 gcgttccctg ctggactgtg gcgacagcgc gaatgaatcc gtgttgcaga cctgccttca
     6601 ggcctacggt cataaggtat cccgtgatgt ggtgcgcacc cagcttgcat ggctgcgcga
     6661 gcaggggctg gttcgtctgt ctgatgtggg cggctgttac gtggctgaaa tcaccggcag
     6721 cggcgaggat gtggcaaacg gcctgtccgg tgtgccgggc gttaaaaaac cccgtgcgag
     6781 ggactgaaca tggcccgaaa actgaagccg ttaagtcgtg gagaacgggc cgtcgtacgg
     6841 cagctggcgt actgccttgt gctggcagac attgagcaaa acgccattgt ccgtgcatac
     6901 gaaaaacata caggaaagcc ctggaacccg gatgcaccgg atacccccgt gaaacgtgcc
     6961 ctgcgttcat caccggcata tgcgcggctg tggaagctgc caggtaagga tattcagtcc
     7021 gttcgtgaag aaatatacgc tggtctgaaa acacagagga cagaagatgg aaaatgaaca
     7081 acgccccacc cgtggccgtc tttccaaagt ggatttactc ccggacagca tccgtgagca
     7141 gttacaccag atgctgcggg aaaagcggca cacgcaggaa gaaatccgcg aagccatcaa
     7201 tgccctgatt gacgaacaca acctgccggg ggagatgcag ttaagccgga cgggcttaaa
     7261 ccgctatgcc agccgcatgg aaaaagtcgg ggcaaaaatc cgtgcctccc gcgaaatggc
     7321 cgaagtctgg gcggcaaagc tgggttccgc gccgacgtca gacgtcggca ggctgctgat
     7381 ggagtttgta aaaacgctgg cctttgaaac gtccatgtcg atgacagaag acgacaaacc
     7441 tgttgcaccg aaggcgctgg ggcaactggc gctggttgcc cagcgtctgg aagcggcagc
     7501 catgaccagc cataaacgcg aaaaagcaat ccgcgatgcg tttgcgcagg aaatggcaga
     7561 gaaaaccgaa gagctggtca gagcgggcgg tctgtcaggc ggtgcggctg acaccatcaa
     7621 acgtgaaatt ctggggatta gtgtatgaca cagatgaacg cattcagtga atccgatgtt
     7681 ctgctgccgt atcagaaacg ctgggtggcg gatgattcaa aactgaaaat cgccgaaaaa
     7741 tcccgccgta ccggtttaac ctgggcggaa gcggcagatg cggcactgac cgcatcactg
     7801 aagaaggaaa acggcgggcg cgatcacttt tacatcggtt cgaacaagga gatggcccgc
     7861 gaatttatcg acgccgtggc gatgtgggca aaagcgttta atgcagccgc ggaagaaatc
     7921 cgcgaggaag tgatcaccga cgaagacaag gacattctga cgttcgtcgt gtacttcgcc
     7981 agcggattta aggtcaaggc gctgtccagt aatccgggca acattcgcgg gatgcagggg
     8041 aatgtcacca tcgacgaagc ggcgtttcat gaaaagctgg atgaggtact caaggcggtc
     8101 acaccactga ccacgtgggg cggtaaggtt cgccttatct ccacccatga cggcgtggac
     8161 aacccgttta accagctgat tcaggaaagc cgcgcgggca aaaaagatta ccgtattcac
     8221 accatcacgc tggatgacgc ctgtaatgac gggctgtacc ggcgtatctg tcaggtgcgc
     8281 ggcatggtgt ggtcaccgga agccgaggtg gaatggaaag aaggcctgct gcgaaatacc
     8341 gccacccgcg aagatgcgct ggaggagtat tactgcgtcc cgaaaaacgg cggcggcacg
     8401 tatatccccc gctcactgcg tgaacgtgcg gcccgtggca ccggaaaagt gttgcgcttt
     8461 accggcacac cggaatttaa cgcactgacg gaaggccagc gccgggcaga gatcagggaa
     8521 tggctggaaa cggtggtgcg ccctgagctg gaaaaactcc cgaagaatct gcgccactgt
     8581 ctgggggaag actttgcgcg ttcgggtgac ctgaccgtgc tggccccggt gacggtgaac
     8641 gacgacacca cccgcgaggt gccgtttctg gttgagcttg ccaatgtgcc gtttaaacag
     8701 caggagcagg tgctgttctg gctttgcgat cgtctgcccc gtcgtgacgg tatcaaaatg
     8761 gatgcgcggg gtaatggtca gtatctggca gaacaggcgg cagaacgata cggcgacgag
     8821 gtggaacagg tgatgctgtc cgtggcgttc taccgcgaaa acatgccccg tttccgtgcg
     8881 gcgtttgaag atgatgagct gatccttccg aagcatgaag atgtgatcaa tgacctcggg
     8941 gccattcagt tactgcgtgg cgttcccggt attgacgatg cccgtactaa aggcagtgat
     9001 ggccgcaagc gtcacggcga cgccgctgtg gctattttcc tgggcttcct tgccagtaaa
     9061 gatgactgcc accgttacga gctgcaccgc ctgaaccgcc cggcgaaacc ggaagaaggc
     9121 aacgcgcgcc gccagatgaa gctgacacgc ggcctgaaaa atgagggagg tttactgtga
     9181 acctgaaaca actggccggg gccgttcgcc gtctgctgaa tccggcaacc ggtgaggaag
     9241 acacgctgaa aaaagaggcg ctcaacgaag cacaggcccg tccgcgtcat gccggtgtgc
     9301 gtccgacctc gccgggtatc agcgtcgcct ccgggctgaa ccccggcagg ctggcaggca
     9361 ttctgcgcaa tgccgccgac ggcatcacgc gtgatttttt tatccttgcc gaagagatgg
     9421 aagaacgtga tttgcattac gcctcggtgc tgcgcacccg caagctgaca gtggcaggta
     9481 tcgaaccggt ggtggtggcc gccagtgatg acgatgccga cgtgcagctg gcggacgcca
     9541 tccgtgcgct gatggaagcc ccgcaaatcc ccgaactgat gtttgatttg ctcgacgggc
     9601 tgggaaaagg cgtggcggtc tgtgaaatcc tgtgggacac ccgcaacaac cactggatgc
     9661 cccgtgatta tgagtgggtt gatccccgtt ttctgaaagc cgaaaagccc acgctgcgcc
     9721 agttccgcct gctgacggac gatgaaccgg tggatggcgt accgctgaca ccgggaaaat
     9781 tcattgttca ccagccgcgt ctgaaatccg gtctgccgct gcgtaacggt ctggcccgtc
     9841 tggtggctgt gatgtacatg ctgaaatcct ttaccgtgcg cgactggtgg gcatttgcgg
     9901 aaaaattcgg cattccggtt acggtgggga aatacggtcc caacgccacg gaggagcaaa
     9961 tccgcgtgct gattgatgcg attgcctcca ttgcctcgga tgccgggtgt gccattccgc
    10021 agtccatgca actggagatg caggaaaccg ccagccggaa taacggcggc gcactgttcc
    10081 gtgaaatggc ggaatggtgc gatgcgcaaa tcagtaaggc tgtgctgggg cagaccatga
    10141 ccacggataa cggcagttcg cgttcacagg cggacgtgca caatcaggtg cgcatggaca
    10201 ttgtgcgctg ggacgcgcga cagctggcta acacgctgaa tgaatatctg gtgcggccct
    10261 acatcgaggc caactacggg ccacaggcgc actacccccg tgttgttctg cgtatcagtg
    10321 aagccgaaga tctgaaggcg ctgacggatg ctctggtgcc gctgattgac cgggggatgc
    10381 gggttcagga atcggagctg cgggaccggt tcggcctggc tgagccagac gaaggggctg
    10441 atgttctgca tccggtttcc gccgggatgc cgggtgatat ggcgatgaac cgcgagcgcg
    10501 tcgccctgaa cagggaacac cccgacgaac tggcacagat ggtggatgat gccctgcgtg
    10561 actggcagaa aaccggcgag gcgttcacga acccggtgct gacactggcg caggagtgcg
    10621 acagttttga tgactttctg aaacgcctgc ctgagcttca ggaaacgctg aacgcggacg
    10681 actttgccct gcaactggcg gaggtgtgct ttaaggcgcg tgcgctggga gacactgctc
    10741 atgcgtgaaa ccctcatccc gaaagaggcg ctggcgtggc tgaaggcgaa gaagctgcgc
    10801 cccggttttg attaccggga tgtatggcgg gaagagcacc ggaacagctt cacggtggca
    10861 aaaatgctgc aactggattt gttgtcggat gtgaaagccc ttgtggaaga cgccctgcaa
    10921 agcgggcaga cgttcagcga gttccgggag atgctgcaac cgttgctgat aaaacgcgga
    10981 tggtggggcg tacaggagat ggatgatccg ctgacgggtg aaacccgcac cgtgcaactg
    11041 ggcagcgacc gccgcctccg cacgattttt gataccaaca tgcgcaccgc ccgcgcggcg
    11101 ggccagtggg aacgcattca gcggacaaag caggccatgc cgtatctgat gtacgagctg
    11161 gggccatccc gtgagcaccg ggtggagcat gtgaaatggg cgcgtctgtg tctgccggta
    11221 gatcatccgt tctggcagac gcattttgcc cctaacggct ggggctgcaa atgcaccatc
    11281 cgtcaggtca gccgtggtga gtatgcgcaa ctggcggcac agggtaccat tcacaccgaa
    11341 gcgccggaaa tcagaactgt ccgctgggtg aacaaacgca cgggcgaaga ggaagatgtg
    11401 ccggaaggga ttgatccggg ctggaactac aatcccggta taaaccgtga gcaggagctg
    11461 gcgcgccagc tggcggccag acaggcccgt tttaacagtg aataacccac cccgccgtaa
    11521 atacccctga aacgcatcag aaacgcgttt tttattctga tggcatgaat gtacaccctg
    11581 gcttttttga agcgtctgtg gcgtttttga aggggttttg aagggggtat ttccccgttt
    11641 tcagtgaagc cggtaaatcc ggcttttttt ctgccttccg catactgacc gtcggtaacc
    11701 ccagacgacg gagactgaca tgcaaccgga actgctggcg ctgtgttttt ccctgccaga
    11761 acccatccct gagctgacac ccgctcaact gccggaatgg cttgaactcg ttcctgcggg
    11821 tgagttcacc gggcgcgatg gccggacgtg gatgaaccgc aatccacatg aggtggttgc
    11881 ccgttcgtcc gacatcaaca ttccggtgga cattgaacat gccaccgaaa ttaaaggccc
    11941 gcggggtgaa gaggctccgg cgtatggctg ggtggaagca ctgcgggtga cggacagcgg
    12001 caccattgaa gggcgtgttg tctggagtga gtccgcccgg tggatgctga gcgagcgccg
    12061 ctaccgctat tacagcccgg cgtttttcca tgacgcagac ggtgcggtga cgcgcctgtc
    12121 cagcgtcggg ctgaccaaca aacctaacct ggattttcct gcactgaata cggagaaaaa
    12181 cccgatgaca gtacctgtgc aaatcaccgg cctgcttggg ctggctgaat ccgccacggt
    12241 ggacgatacc gtggccgcca ttaaacaact tcaggagaac gaacaggtgg cgctgaaccg
    12301 cgcacagaca cctgacctga cgaagtttgt gccggtggaa acccacaatc tggcactgaa
    12361 ccgtgccgaa accgcagaaa aacgccttca gcaactggaa gagaaggaag ccgaggcgct
    12421 tgtggatgcg gccatcgggg ccggaaaagt cgccccggca aaccgtgaca tgttccttgc
    12481 cacctgccgc acggaagaag gccgcaaaca gtttgcggag tacaccaaag gtgcacagcc
    12541 gctggttaac aacgacaagc ccagccaggg caaggataaa cccgcgcaga cactgaccga
    12601 tgccgaactg gcgatgtgtc gcagcatggg tattaccggg gaagagttcc tcgccgctaa
    12661 accgaaacag gagtaagtaa cacatgtcac agactatcag ttctgaaatc cttcatgcac
    12721 tgaccacctg cctgagcgcc gcctttaccc gtggactgag tggtgtcgaa ccgcagtgga
    12781 atcgtattgc ttctgaggtg ccgagttcgt ccgcctccaa cacttacggc tggatgaaag
    12841 atttaccgga catcaaagaa tgggtcggtg agcgtcagct ggcaacgctg gacggttatg
    12901 gttacaccat caccaacaaa ctctgggaaa gttccattcg cgttaagcgt gaacacattg
    12961 aagatgacca gattggtcag tacagcatta ccgccgaacg ctatggccgt atgactgctg
    13021 tgttcccgga caagctgtgt tacgccctgt tgtgtgccgg ttttaacacc ctgtgctttg
    13081 acggccagaa cttctttgat gaagaccacc cgctgggtga cggtacgtac agcaacgttg
    13141 tcggcacccc ggcatcagac aagggcgaac cgtggttcct gattgatgag tcgcaggtgc
    13201 tgaaacccat catctggcag acgcgacgcg cctttaagtt tgaagccctg gacgatctga
    13261 acagcgagca caccttcaag aaccacgagt tcctgtacgg cgtggacggt cgctgtaatg
    13321 cgggcttcgg cttctggcag accgccgtcg gttcccatgc agcactgacg gtggagaact
    13381 acaaaaaagc caatgagttg ctgcggggca tgaagggcac caacggtgag ccgctgggca
    13441 tccgcccgac cacccttgtg gtggggccga aaaaccgtgc ggacgcgaag ctcattattg
    13501 acgccatgct ggttaacggc ggcgattcca acatctggta caaggatgtg aagatcgtgg
    13561 acagcccgta catcaccacc ccggcataac ccgtcatccg taaaccgcag ttaaaaggtg
    13621 ctgtgaatgc ccttttaact gccttttaaa aggcagagtc atgagtgaaa aagcagaaac
    13681 caaaggcgcg aaagccgcaa agaacagcgc tgcacaggaa aacccggcac cgctggcaga
    13741 cgttattgtg gctgacggtc aggcgaatga accacgtccg gctgaagacc cggttgctgt
    13801 acagggtgac gcccctgtcc ggctgaacgt cagggccgtg tctgaaaacg ggttctggcg
    13861 ctgtggccgt ttctggtcac atatcggtga ggatgtggcg gtgagcgctg cggttgccac
    13921 ccgcctgatg gcagagccga atctgattgt ccgggaagcg gagaaaggct gatgggatac
    13981 atcacgcagg aagacctgtt acgcgcggac ggcaatcttg tctggaacat ggcgattaac
    14041 cgggaaacca acgggctgga tgaagacaaa atccgtcagg ccatcagtga tgcggaagcg
    14101 gaaattgatt cgtttctgtc ccgccgctac cagctgccgc tgggggtgac ggaaatcccg
    14161 cgcccgctgc aacgcgtggc ggtatcgctg gcgttttact ggttatcaga gcgtgacaat
    14221 caaatcacgg agctgatcca gaaacgctac gacgacgcca ttaaaaccct gcgtgaaatg
    14281 gcgaacggca cccgtgacct gggcctgccg acgtatgcca ccccggcaga aaccgacaac
    14341 gggaaaatta ttgtggtggg tgccaatgcc cggctgttca cccgtaacaa cctgaaaggg
    14401 gtgctgtgat ggggatttct gtacaggtca gcggtgacca gcgtctggag gatatccgcc
    14461 gcgccgttga aaagctggca gagggttcat tgcagtcaga gctgctggaa agcatcggtg
    14521 cggtggtgga atcacagacc cgccgccgca tcatcgatga gaaaaccagt ccgggtggcg
    14581 aacgctggcc ggactggtct gacgggtaca aaaagacccg ccacggcaac cagagtctgc
    14641 tgcgcggtga aggccatctg ctggagagta tccagtacat cgtggaaaac cgcgtggtgc
    14701 gtatcggttc accgctggat tatgcccgca tcatgaatga cggcttttcc ggcagcgtgc
    14761 cggtcagcgc ccacaaacgg cttatctcgc agtgtttcgg gcgggcgctg aaatatccgg
    14821 tctggcagac cgtcggtgca catcaccgca tgatgaacat tccgcaacgt gaatttctgg
    14881 ggctgtcttc tgccagccag caggagctgc aacaggttat cagtcatttc tggaaggagg
    14941 ttctgccatg acagaacaac gccctgaact gcgcacaccg gggagcaccg ttgcggccgc
    15001 agagcgcatt gtagcctggc tgcagacggc ccttcagggg gacacccccg accgggctga
    15061 tgtggtggag cgtcacatcg gccagttcaa cagcacggat gaagtgaaac gctatctgtc
    15121 cggtcgcacc ggctgtatcc gggtagctgc cctgcgtgtc cgggatatca acccgcgcgg
    15181 cgggctgtcc ggtctggtta cctgggtggc ttacatcatg gcgacggatt cgtgggggta
    15241 ttcccgcgac gtgcgctgtg aagtgctggc cgggaaggtg atcaaacgcc tgctgtcgtc
    15301 ggatgccacc gggggcatgg gggctgaacg tatggccgct gatgtgcggg cagacaacat
    15361 ttactccgtc agcctcgacg ggctgggtgt caccatgtgg gcggtgactt gggagcagga
    15421 attccggctg gatgaagaga ttgatctcgc cgcgctcccg gatttcctgc gcctcggggc
    15481 gacactgcgc agcggcgaac acactgaaat taacgacgtg atccatgtac ggggtgacga
    15541 tggaacagaa actgattaag ccagcgcggg aaaacgtccg tgtccgtaaa ccggatggcg
    15601 cacatttatc cccggaaggg gaacgtctca acgtctgcgc ttactggctg cgccgtgaag
    15661 ccgagggaga tgtggaaata accacacttc cgaaaaataa caacaaaacc agagggaaaa
    15721 aataatgtcg ctgggttcca ttcctgatga tatccgcgtc ccgcttgtct ggatcgatat
    15781 cgacaattca caggcgctgg agggtgcatc cgcgcaaagc cgaaaaattc tggtcatggg
    15841 ccatgcggta tcgtccggca gcgcagacgc cctgtcactg acccgtatca ccagtgacag
    15901 ccaggcagac cagctttatg gcaaagggtc gatgctggct gaaatgctca aaatgctgcg
    15961 tcgtgccaac acgtacacgg aaacctgggc aatgccggtt gccgcgcctg aaggcgctgc
    16021 cgcaaaagcc acgctgaccg tgctgggtac agcgactgaa gccggaacgg tggcactgct
    16081 gattaatggt gtgtccgttc aggtgagtgt gagcgccggg gctacgaagg aaaacattgc
    16141 aaaagccatt gctgatgcgg tgacgaaaaa gcctgccacg caggtggccg ctgcggtgaa
    16201 ggatgatgcc acggataccg tggagctgac cgtgaactgg cacggcgtca ccggcaacgg
    16261 tgccgacgtt cgcctgaact accacaccgg tgaagccttc ccggcaggtg tgaaggtgac
    16321 cgcaaccgcg tttaccggcg gcaccgggac accggaaatg gcagacgccg ttgcggccat
    16381 cggcccggag tggtttaccg atatcatcgc cccgttcacc gacacgaaaa gcctgaacac
    16441 cctgcgtgat gaactgctga accgctgggg gccgctcaaa atgatggaag cgcagctgtg
    16501 gacggcgttt cgtggcacac acggggaaac cggcacgttt ggtgaaaccc gcaatgactg
    16561 gctgattagc tgtatcggca ccaacctgtc accgcacccg gcgtggatgt gggccgcgtc
    16621 atacggcgca acggcagcgt atcaccttgc cattgacccg gcgcgaccgc ttcagacgct
    16681 ggtcctgacc ggcattctgc cgcctgcgcg taatgttcgc tgggatatgc cggagcgtaa
    16741 tctgctgctg catgacggca ttgccacgca catggtggac gccggggata acgtctgcat
    16801 cgagcgcgaa atcaccatgt accgggttaa ccagtacggt gatgcggatg tgtcgtacct
    16861 tgatgtgcag tcacccgcca cgctgggccg tatccgttac atcatcaaga accgtttcac
    16921 gaaccgttat ccgcgccaca agctggcgga tgatgacgtg ctggactcgc tggatgcggg
    16981 gcagccggtg atgacgccga agctgtgcac cgcagaactg ctggatatct gccagaccga
    17041 acttctcccg gcgggccttg tggagaactt cagcgattac aaagacacgc ttcaggtgac
    17101 acgcgacagc agcgataaaa accgtctgaa ctttatctgc catccgaatc tggtgaacca
    17161 gttgcgtgta ctggcaggcc tgattcagtt caagctttaa gggggccaca tggcaaaaat
    17221 tcttggcatg gcgaccattc gcgtgaatgg ccgcgaaatt aaaaccgagg ggaaatccac
    17281 gctgaatccg ggcggcttca gccgcaccca gcatatgggc ggcggcaagg tctggggtat
    17341 ctccagcaag atggcctcgc cgtccatcaa ggtgaccatt gcggcggcag cggatatgga
    17401 cgtgattgaa atcagcagct gggaagatgt cacggtgatg ttctacggca acaacggcct
    17461 gaactacatg atgacgggtt cagccacgga taacccggcg gagctggacg aggattccgg
    17521 caccatcagt gcgaacttta tcggtgaaaa atgcgtaaag gtgtgacatg gccgcgatgg
    17581 aatttgaact gaaacacggg cttctgaccg gcaaaggcac ggcagatgaa accctgcata
    17641 aaaccgtgaa actgcgcgaa ctgaccgcca gtgacgtgat tgatgcgcaa ctggccgcag
    17701 aacgcgtcgt catgggcggg aacggaaagg cggtggccta ctgctcggaa gtgctgatgg
    17761 ggctggagat gatgcgccgt caggttgcgt caatcggtaa tatccccggc ccgctggaca
    17821 tgaaacagct gcgaatgctc cacccggcag accttgaact tatcagcacg aaagccgctg
    17881 cgctggatga aatgcttgag gaggtggcaa cgcgggggcg aactgatgcc gctggcggcg
    17941 gctctgatga acctgctggt taacctgtct tcgcgattca gcattcagta cctggaacag
    18001 ctgcccctgc ggcagctgtt ccgcctgata aagcaactgg agaaacagca tggcaacagg
    18061 taaccgtctc agcacggaaa tcatgatcaa ccttgccggg aacctgaccg ccaaagcccg
    18121 gcagtacggc gcaaacatgt cgcagttcgc ccggaacaac cagaaggcca tgcgccttgt
    18181 caaagccaca acggaggccg caggacgcgg ccttgatacg cttggtaacc gctacacggc
    18241 aatgatagcc gggcttggca gtagtcttac catcaaacag gttgccgatt ttgatgcgca
    18301 gatgcgccgg atgggtacgg atgcccagtt aaccactgaa caggtaaaga cgcttcacga
    18361 caaaatcagg gatgtatcga accagaaaga tattcgtatt gatgcttctg cgcttggtca
    18421 gggggccagc gaacttctgg ggaaaaccgg tgattatcag tatgtggtgg acaacctccg
    18481 caacatgggg ttgatgatgc aggcgttcgg tgttgacgga caggttgcag ccgggctgat
    18541 ggcccagttc tgggaaaaaa acgtccgggg tgctgatgcg gtcaataaca tgatggacag
    18601 gctgtattcg cagttcgcag tggggtctgt aagcgttgcc gatgttgccc gcgctgcccc
    18661 gaaactgttt tccatcattc aggatcaggg accggaagcc attgcccaga tgggggcatt
    18721 tgctcaggtg ttcgccaaaa acaaaggcag cattgatgag acggtaacca gtattcaggc
    18781 catgtatgct tccctgtcgg acaaaaagaa catcgagttc ctgaaaaaaa acggcattga
    18841 cgtttttgtc aaaggcacaa aagacatcaa aaagccctat gagctgatga tggaaatcct
    18901 taaacgggcc aaatacgacc cgctgaaact tcaggatgtg tttgatctga ccggaatgca
    18961 ggggattaag gcgctgctta accctgaaaa ccggcaactg atggaacaaa tgatttacgg
    19021 cacgattgag ctgggttcca cgcaaaaggc cgcacagacc aatgccgaag gctttaatgc
    19081 cgcaatacag tcactgaata acgaatggca acgttttgct gaagggcaac tggcaaagcc
    19141 cgttcaggac ctggcggatg cgctcaacag cgtggaccag aacactgccc agaactggtt
    19201 gcaggtcggt aaatatatgg cgatcgcgct gggcggcatt atcgccatca gaaagacgta
    19261 tcagttcggt aaaaccatcc acgacatcat gaatcccaaa gggaaaggca aagggatacc
    19321 cggcagcatt acggatgttt tcggctccgg tgtgatgccg gtttatgtgg tcaatatggg
    19381 cagtggcggg atgaagggta ataccggcgg tctgccggat gcgccggatt catcgcgcaa
    19441 tccccgtaac tcccgcaacc cacgtgggcc aaagacgcca gcgctggtta caccacaaac
    19501 tgcatcagga tttaatctcc ttgacatggt gctctctgct ttccctaaga acaaagagga
    19561 agcagatgcg ttaatcgcac gggttcagga aaacaacaac cgcccgacgg tatggacgga
    19621 tatcaggaac tggtttaact ctctggaaaa aaagaatatt gccgacccgc gaccgtgggc
    19681 tggtatgcaa cccactcaga attatccttt ccttccgcaa caattgcagg gggaaatccg
    19741 tgtggtggtg gaaggtgatg cccgcgtgaa aagcgtcaga gtggatcaac ccggcgtcag
    19801 actcagtgcg caggctggcg tcaccagcgt ggagcagggt taatgacaac gagcaacggt
    19861 aaatgggacg ggctgcgcga cgcctcgttt cgtggcgtcc ccttctttct ggtggatacg
    19921 gagggcaccg gtggtcgtcg tgccattccc cgtgcttatc cccggcgtga aaccgcctgg
    19981 acggatgata acggggccgt tccggggcaa cagcagatta acgcaaagct gccaggtaaa
    20041 aacttccggg atgatttaaa cgccctttta gatgcgctca atacccccgg ccccggcgag
    20101 cttgtccacc cgtggttcgg gatacagacc gtacaggttg gcaaggtcac ccatcgcctc
    20161 agcacggagg aagacggcat tgcgtatgtc acctttgaag tgtttgaggc aggcgagcgc
    20221 ctgttcccgt ctgcggcgga taacacgcag caggaagtgc tgacaggcat tgatgcggtg
    20281 aaagccgcca ttgacgatgg cgactggttc ggcgcactcg acgggctggg tgacatggcc
    20341 gacagttttc tggccgatat ggaaaacctt gtggctaacc tgcccacgtt accggcagca
    20401 ctgaatcagt ggatggacag gctgaagcat tttaaggcga tggccggaac cctcattgcc
    20461 acaccggggc gtctggtcag tgagctgtca gcacgggtgg aagatattgc gggccttgtg
    20521 acggaaccac ccgaagcact ggcggtttac acgacattac gcaaccagtg ggccggagaa
    20581 cgtgccaggc agattgccac cggcgcactg ccggaagata tcaccgtgaa gcccggcagc
    20641 gtggcagacg gcaaccccgg ctttgccatc gggctgtcac cggattatca gccggtatct
    20701 gacagcctgc aaaagaacat tgacgacttc cgccaggtgg ttgtgctggg agccctgctg
    20761 ggacaggcca gtgccgtggc ctcgatgacg ttcgataccc gtgatgcggc gttatctgcc
    20821 ggtgacacgc tggcggctga actgcatgag caggcggtgg cggcggtgga aaacaaccag
    20881 cggacattgt ggcgaacgct gcgcgattta cggcaggccg tgattacgga tgcccgcgag
    20941 cgtgccgccc gtctgccgga aacccggcag gtgacgctga ccacaaccac ctctgccgca
    21001 ttgctggcat ggcgcgagca tggtgacaca cgccgacggg atgaaatcgt gcagcgtaac
    21061 cgcctgcgtc atccgtcatt catcctgccg acgcaacctg tggagatcac cgactgatgg
    21121 aatccgtgat tcttaccgtt gacggcaaac tgtgggaagg ctggacggaa atgtccgtca
    21181 gccgttccct gaaggcgatt gccggtgagt ttgaactcag tgtgacaacc cgctggtcag
    21241 cggcggcacc gcgcgtgatt cgtgaggggc aaccctgcac ggtcaggctg ggcgcggata
    21301 ccgtgctgac ggggtatatc gatgatttta ttcccggcta tgacgctgaa aacgtggaga
    21361 ttcgcgtcat ggggcgcgat aaaaccggcg acctggtgga ctgctctgtg gtgcattcgt
    21421 ccgggaaatg gaaaggcgtg cggcttgaac aggtggcggc tgatgtctgc cgcccgttcg
    21481 ggataaccgt catcacggaa accccgaccg gggaggcatt tgcgtctgtc gttctggaac
    21541 agggtgaaac gggctttgaa ctgctcgacc ggctggcaaa acagcgcggc gttctgctga
    21601 cgtctgacgg cgcgggtaac ctgattatca cccgcgcctc ttccgttcgc gcgggcgtgt
    21661 cactggtgct gggaaaaaat atcctcgccg cccgtgggcg cttcagctgg cgggagcgta
    21721 acagccagta catcatcaag ggcaccacca gtgccggtgg caaactgtgg gacagccagc
    21781 ctgccccgat ggtgggcggt cgccagtaca tcaccgaaga cccggaaatt aaccgttacc
    21841 gcccgcgcat tctggtcaat gaagacagcc tgaccgtggg cggggccagc attcgcgggg
    21901 aatggtacaa agcccggatg ctgggagagt cccgcacgac ggaaatcacg gtggcaggct
    21961 ggcgcgaaca gggcgacagc ggcccgctgt ggcagaccaa ccgccttgtc gatatcgacg
    22021 acagcattca gaacctgaaa accacctggc tgatatccgg ggtgacatgg acggatggcg
    22081 cacaggggag gatgacggtt ctggcgctgg ttccgcctga atcactggac atgccggaaa
    22141 tgaaagcgaa gacgaaaaaa acaaaggcgg tggccacatg ggattaaacg caattgcccg
    22201 ccgcctgcga ctgatggttg accgcgctgt tgtccgcctg gtgtctgacg gtctggcacg
    22261 gcagaacctg caaattcaga cgctggcaga tgccaccgat gacgacgtgg aacgcttcca
    22321 gaactacggg tttacgtccg ttccgccgga aggttctgaa gccattgtgc tggccgttgg
    22381 cggacggcgt gccgggctgg ttgccatcgc ggtggaagac aagcgatgtc gcccgaaagg
    22441 actgtccccc ggcgatgtca gggtgtatca ccgggacggc aaatcacaca tcaccctgaa
    22501 ggaaaacggc gtcattgaaa tcacaggaga acaggtagac gtttcaggga aaacggtaaa
    22561 tctcaccgcc gacgaattgc tgaatattat cggtaaacaa atgaaattcg tcggtccctg
    22621 tgaatttact gaagatgtca gaatcaaggg taaatcattc agcgaacaca ttcataagga
    22681 cggcgacaat gagaagacat caccgcccgt atgacgacag aaatccgctg gaataacatg
    22741 ctctcacggg gtgacatcac cgtcacccat aacggcctct cacgggatga ggggctggtc
    22801 actcaggttc ttatctgcct tttcaccgat gcccgcgctg atgacgatga tgtcattcct
    22861 gacggctccg gcgacccgcg cggctggccg ggcgacacgt acagcgattt ttcgtggggt
    22921 tcccgcctgt ggctgcttga gcgtgaaaag ctgacggaag atgtccgcct gcgcgtggaa
    22981 gattacgcgc ggctgtccat gcagccgctt ctgcgtgccg gttacgcccg taacgccacc
    23041 gtcacggcca gcatcatcat tcccgaccgt attgcctttc tggtggtgtt aacccgcccg
    23101 gacaaaacca cactcagaat cgaaatcacc cgtcgatggg aggccaccat taatgccgta
    23161 tgaaatcccc acgctgggca aactgattgc cgacggcgaa aaagatattg cgtatgaact
    23221 cggcctgcaa aagctgccgc ctgtcagtgt tgagcaggcg ctgaacgtgt cattcagcag
    23281 tcaggtccgg gatttatacg accatcagag ctggattaag gaccagataa tcccgtccac
    23341 cacgtctgat gatgaaacca ttatcaaaac agcaaagtat gaaggggtta tccgcaagca
    23401 ggccaccttt gccagcgggc gggtgacctt caccagtcag tccccccttc cggcagaaac
    23461 ccggatgcag tccgacacga accaggtgta tcaggtactc acgtccggcg aggtgcagga
    23521 cggcgaggtc accgtcatcg tgcaggctga agaagccggt gtggcgggca atcttgctgc
    23581 cggtgccgta ctcaccctgc tgtccccgct gcccggaacg gccagcacag gcgcggtggc
    23641 tgaaggtggc atcaccggtg gcgcagacac tgaatccatc gcagcgctgc tggaccgtct
    23701 gctgtatgtg cgccgcaatc caccgacagg cggcgcgttg catgactacg tgatatgggc
    23761 gcgtgaagtg ccgggcgtca gccgcgcgtg ggcgtgggat gcatggcacg gccccggcac
    23821 ggtcgggctg gcatggcttt atgatgaccg ggaaaacatc gtgccaaccc gcgaagacct
    23881 gaagacaatg gagcagtatc tgttctgcca caaacacccg gccacgggcg tgatggtggg
    23941 caaaccgggc ggcattgagg tctggccggt acaggtcagg ctgaagaaag tcagcatatc
    24001 catccgtctg acaccggaca gcctggcgaa caggagcgcc gtccgggcaa acctgaccgc
    24061 gttacagaag acactggcac ccggtcagac gctgccggtg tcctcgctgc gcacggccat
    24121 cggtatgacg tccggcatca cggattactt ccttaacatc ggggaggaca ccaccagtga
    24181 tgtggatgag cttatcacca tcggggaggt gacatggctc acagcgtgac ggaatggctg
    24241 gccgcactgc aacaggtcat gccacggggt aaggcatggc cgcgtgataa cgacgcggat
    24301 ttaaaccgct ttttaagggc gcttgcagaa cgtttaaacc gcactgaata cgacgcctcg
    24361 cgtctgcatg tggagatgcg cccggaaacc acgctccggc tgctgccgga gtgggagcag
    24421 tatctggcgc tgccggaatg cggaattgcc cccaccacca gggaagcccg ccgccgtgcc
    24481 gtggtggaga aataccgtcg caggggcggg ctggcaacct ggcagactga agccgcagca
    24541 gcggcgctgg gcttcaccat taaggtgaca gccgttctgc cgcaccactg tctgcgcgac
    24601 tgcatgtatc cgctgcatcc ggcgcggtat cgctggatgc tgaaagtgaa cgtcccgggt
    24661 aaagatgccg ggcggtttac ctgtattgat ggcgtcatga caccattaat cagcgaacgg
    24721 acccgcgagc tggaatgcct gctgaagcat taccggctgg cgggcacgga atatgaatat
    24781 tattacaccg gagaataatt tatgttttat gtggataacc cgacgggtgt gccggtaatg
    24841 ccacctgtcg cggcagaatt aagcaaaaca acgctttatt tcaccgaagg cgggaacggt
    24901 attccgccca cctatccggg gccggactgg tttaatatta ttcagtctga attactggaa
    24961 atactccggc aggcaaatat caaaccggat aaaaacacaa ccgaccagat tatgacggcg
    25021 ctgaaaaagc tgtttattac gaacagtggt tcagccggtg ctattgccgg attaaccggt
    25081 cagaataata cattcccgta ttttaccggc aaagacacaa tggcattaac accgctcagt
    25141 gcttttgtgc gcggtattct cggcaaagag agtgcaacag attttgcaga tgcactgaaa
    25201 gtcataaaac aatcaggcgg cacgatgac
//
LOCUS       DAZRUF010000036        25156 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZRUF010000036 DAZRUF010000000
VERSION     DAZRUF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25156
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.036"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(134..274)
                     /gene="yjjY"
                     /locus_tag="ACHXOL_001204"
     CDS             complement(134..274)
                     /gene="yjjY"
                     /locus_tag="ACHXOL_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR6808233.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            370..1086
                     /gene="arcA"
                     /locus_tag="ACHXOL_001205"
     CDS             370..1086
                     /gene="arcA"
                     /locus_tag="ACHXOL_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR6808234.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1146..2498)
                     /gene="creD"
                     /locus_tag="ACHXOL_001206"
     CDS             complement(1146..2498)
                     /gene="creD"
                     /locus_tag="ACHXOL_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR6808235.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2556..3980)
                     /gene="creC"
                     /locus_tag="ACHXOL_001207"
     CDS             complement(2556..3980)
                     /gene="creC"
                     /locus_tag="ACHXOL_001207"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR6808236.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3980..4669)
                     /gene="creB"
                     /locus_tag="ACHXOL_001208"
     CDS             complement(3980..4669)
                     /gene="creB"
                     /locus_tag="ACHXOL_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR6808237.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4682..5155)
                     /gene="creA"
                     /locus_tag="ACHXOL_001209"
     CDS             complement(4682..5155)
                     /gene="creA"
                     /locus_tag="ACHXOL_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR6808238.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5366..6235
                     /gene="robA"
                     /locus_tag="ACHXOL_001210"
     CDS             5366..6235
                     /gene="robA"
                     /locus_tag="ACHXOL_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR6808239.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6232..6879)
                     /gene="gpmB"
                     /locus_tag="ACHXOL_001211"
     CDS             complement(6232..6879)
                     /gene="gpmB"
                     /locus_tag="ACHXOL_001211"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR6808240.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6931..7452
                     /gene="yjjX"
                     /locus_tag="ACHXOL_001212"
     CDS             6931..7452
                     /gene="yjjX"
                     /locus_tag="ACHXOL_001212"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR6808241.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(7537..7863)
                     /gene="trpR"
                     /locus_tag="ACHXOL_001213"
     CDS             complement(7537..7863)
                     /gene="trpR"
                     /locus_tag="ACHXOL_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR6808242.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7953..9890)
                     /gene="sltY"
                     /locus_tag="ACHXOL_001214"
     CDS             complement(7953..9890)
                     /gene="sltY"
                     /locus_tag="ACHXOL_001214"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR6808243.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10101..11768
                     /gene="ettA"
                     /locus_tag="ACHXOL_001215"
     CDS             10101..11768
                     /gene="ettA"
                     /locus_tag="ACHXOL_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR6808244.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11929..12036)
                     /gene="nadS"
                     /locus_tag="ACHXOL_001216"
                     /pseudo
     CDS             complement(11929..12036)
                     /gene="nadS"
                     /locus_tag="ACHXOL_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(12075..13307)
                     /gene="nadR"
                     /locus_tag="ACHXOL_001217"
     CDS             complement(12075..13307)
                     /gene="nadR"
                     /locus_tag="ACHXOL_001217"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR6808245.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13328..14710)
                     /gene="radA"
                     /locus_tag="ACHXOL_001218"
     CDS             complement(13328..14710)
                     /gene="radA"
                     /locus_tag="ACHXOL_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR6808246.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14759..15727)
                     /gene="serB"
                     /locus_tag="ACHXOL_001219"
     CDS             complement(14759..15727)
                     /gene="serB"
                     /locus_tag="ACHXOL_001219"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR6808247.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15833..16477
                     /locus_tag="ACHXOL_001220"
     CDS             15833..16477
                     /locus_tag="ACHXOL_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR6808248.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16505..17521
                     /gene="lplA"
                     /locus_tag="ACHXOL_001221"
     CDS             16505..17521
                     /gene="lplA"
                     /locus_tag="ACHXOL_001221"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR6808249.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17553..17816
                     /locus_tag="ACHXOL_001222"
                     /pseudo
     CDS             17553..17816
                     /locus_tag="ACHXOL_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(17977..18696)
                     /gene="deoD"
                     /locus_tag="ACHXOL_001223"
     CDS             complement(17977..18696)
                     /gene="deoD"
                     /locus_tag="ACHXOL_001223"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR6808250.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18776..19999)
                     /gene="deoB"
                     /locus_tag="ACHXOL_001224"
     CDS             complement(18776..19999)
                     /gene="deoB"
                     /locus_tag="ACHXOL_001224"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR6808251.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20051..21373)
                     /gene="deoA"
                     /locus_tag="ACHXOL_001225"
     CDS             complement(20051..21373)
                     /gene="deoA"
                     /locus_tag="ACHXOL_001225"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR6808252.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21500..22279)
                     /gene="deoC"
                     /locus_tag="ACHXOL_001226"
     CDS             complement(21500..22279)
                     /gene="deoC"
                     /locus_tag="ACHXOL_001226"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR6808253.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22537..24087
                     /locus_tag="ACHXOL_001227"
     CDS             22537..24087
                     /locus_tag="ACHXOL_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR6808254.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24059..24922
                     /locus_tag="ACHXOL_001228"
     CDS             24059..24922
                     /locus_tag="ACHXOL_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR6808255.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         5957 a         6946 c         6409 g         5844 t
ORIGIN      
        1 gattattaaa ataatgtgac gaaagctagc atttagatac gatgatttca tcaaactgtt
       61 aacgtgctac aattgaactt gatatatgtc aacgaagcgt agttttattg ggtgtccggc
      121 ccctcttagc ctgttatgtt gctgttaaaa tggttaggat gacagccgtt tttgacactg
      181 tcgggtcctg agggaaagta cccacgacca agctaatgat gttgttgacg ttgatggaaa
      241 gtgcatcaag aacgcaatta cgtactttag tcatgttacg ccgatcatgt taatttgcag
      301 catgcatcag gcaggtcagg gacttttgta cttcctgttt cgatttagtt ggcaatttag
      361 gtagcaaaca tgcagacccc gcacattctt atcgttgaag acgagttggt aacacgcaac
      421 acgttgaaaa gtattttcga agcggaaggc tatgatgttt tcgaagcgac agatggcgcg
      481 gaaatgcatc agatcctctc tgaatatgac atcaacctgg tgatcatgga tatcaatctg
      541 ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa tgttgcgttg
      601 atgttcctga ctggccgtga caacgaagtc gataaaattc tcggcctcga aatcggtgca
      661 gatgactaca tcaccaaacc gttcaacccg cgtgaactga cgattcgtgc acgcaacctg
      721 ctgtcccgta ccatgaatct gggtactgtc agcgaagaac gtcgtagcgt tgaaagctac
      781 aagttcaatg gttgggaact ggacatcaac agccgttcgt tgatcggccc tgatggcgag
      841 cagtacaagc tgccgcgcag cgagttccgc gccatgcttc acttctgtga aaacccaggc
      901 aaaattcagt cccgtgctga actgctgaag aaaatgaccg gccgtgagct gaaaccacac
      961 gaccgtactg tagacgtgac gatccgccgt attcgtaaac atttcgaatc tacgccggat
     1021 acgccggaaa tcatcgccac catccacggt gaaggttatc gcttctgtgg tgatctggaa
     1081 gattaatcgg ctttaccacc gtcaaaaaaa acggcgcttt ttagcgccgt ttttattttt
     1141 caaccttatt tccagatacg taattcatcg tccgttgtaa cttctttact ggctttcatt
     1201 ttcggcagtg aaaacgcata ccagtcgata ttacgagtca caaacatcat gccggccagc
     1261 gccaccacca gcacactggt tcccaacaac agcgcgctat cgacagagtt gagcagtccc
     1321 cacatcactc catccagcaa caacagcgcg agggtaaaca acatgctatt gcgccagcct
     1381 ttcaataccg cctgcaaata aataccgttc attaacgccc caatcagact ggcgattatc
     1441 catgccacgg taaaaccgat atgttcagaa agcgccagca agagcaaata aaacatcacc
     1501 aatgaaagcc ccaccagcaa atattgcatt gggtgtaaac gttgcgcggt gagcgtttca
     1561 aaaacaaaga acgccataaa agtcagtgca atcagcagaa tggcgtactt agtcgcccgg
     1621 tccgttaatt ggtattgatc ggctggcgtc gtcactgcga cgctaaacgc gaggaagttt
     1681 tcccagccgg tatcattgcc tgaagcaaaa cgctcaccga gattattagc aaaccagctg
     1741 ctttgccatt gcgcctgaaa acctgactcg ctaacttccc gtttggctgg caaaaaatca
     1801 cctaaaaaac tgggatgcgg ccagttgctg gttaaggtca tttcgctatt acgcccggca
     1861 ggcaccacag aaagatcgcc ggtaccgctt aaattcaggg ccatattcag cttcaggttc
     1921 tgcttccgcc agtccccttc gggtaaaggg atatgcacgc cctgcccgcc ttgctctaac
     1981 ccggtgccgg gttcaatggt cagcgccgtt ccgttaactt caggcgcttt caccacacca
     2041 ataccacgcg catccccgac gctaatcaca ataaatggct tgccaaaggt gatatttggc
     2101 gcgtcgagtt cgctaagacg cgaaacatcg aaatcggctt ttagcgttaa atcactgtgc
     2161 cagacctgac cggtataaat ccctatcttg cgttcttcca cgttctgatt gccatcaacc
     2221 atcaatgact caggtaacca aaaatggata aaacttcgtt tccgctccac ggttttatcc
     2281 tcttcctgta ccgtataaag ctcggtcaca ggaatagcga taagcggccc aacaagtttt
     2341 tgcggcccgc tggtactttg gcgaatcgca tcttccacat cgctacggta atcggcacgt
     2401 tcgacaatca cctgccgaat cagcattatc ggaatcaaca acagcaatac tgcaccaaac
     2461 agggtggtca ttttccagaa cagggaggat ttcaacatag tcttctcctt tgcaatggca
     2521 gcaggatacg aaggctatgt gggaagaatt tgaagctatg tgaagtgacg gtgaagtcga
     2581 agcgaggcca gcacgccacc ttcctgcacg ttgcgcagcg tgacttcgcc gttaaacaaa
     2641 cgggcgacct cactgacgaa cgccaacccc agaccgctgc ttttttgccc atttgcacgc
     2701 ggcaaagagt aaaagcgttc aaaaatacgt gacagcgcgt agtcaggaat accactaccg
     2761 gtatccagca ccttaagcgt gacgtgttcc tgatccactt cggcgcttag cgttatacga
     2821 ccgctctcgg gggtaaaatc gatggcgtta tccagtaaat tccccagcgc ctgatccagt
     2881 aacgccggtt cagcagcaac attaacctcg gtgggcgtaa catgtagagt gatgtttttt
     2941 tctgccaact gcacggtgcg cgcttcgctg acgcggcgga ataatgccgc cacatcaaca
     3001 gcagtcaaaa cgacttcctg acgattctcc agtcttgcct ggcgtagtaa cgtttccacc
     3061 agtgcctgca ttcgcgcatt ttgcgtcaga atgttgtcag taaaacgagc cactacttca
     3121 ggcggcggac cttcgcgtaa aatttccgcc gcgccacgaa tcgccgccag tgggcttttt
     3181 agctcatggg ttaacgcata aacatactgc tcaatatagt ttttcccttc cagcttcacg
     3241 cgcatacttt ccagcgcctg cgcgagttta cgcagctcgc tactaccgag atcgggaaga
     3301 ggaacgggct tattgtcagt gacggaatca gcatagcgag tgagccgggc aatggagcgg
     3361 ttgatccacc aaaccatgcc tgcgccaatc accagtgcga tccccaacaa aatggcgctg
     3421 gcccataaaa ttcgccgctc gctgcgttta atgaccggag ccatcgccgc gttcggtttg
     3481 cctacgctca aaacgccaat aagccgcgag ccgtccataa tcggcgcggc aacatacatc
     3541 accgaacttt cgggatcggc aggattttgc aacgtgctac gcgcaccata ctgaccacgc
     3601 aacgttagcc agacgtcatt ccagcgcgaa taatcctgcc caacggcttt atttgccgaa
     3661 tcgaacaata ctttgccctg cgcatcggtc atatagacat gatattcatt gcgcactttg
     3721 ttaatgccac cgatattggc gcgaaacggg cgatgttgta gctgattaaa cgcctgcgcc
     3781 agttgcccat gcgttgggtc cccagagagc aaatcgggac gcgccagctc cgccagcaac
     3841 gttgcggtat cgattaacgt cccctccgtt gctcttcgca cgcccggttt aacttctttg
     3901 acaaaaatag ccagtacgaa ccaggccgcc accgccacca gtaaaaaata gcccagcagc
     3961 aaccgcatgc cgatacgcat tacaggcccc tcaggctata tcccatgccg cgatgagtat
     4021 taatcggaga aagatcgggg ttgatggcgc gcagcttggc acgcaacgtt ttaatgtggg
     4081 tatcgacggt gcgatcgtag gtgtcctgcg catcttccca cacgctatcc attagttgct
     4141 ggcgggacca gacgcggcct ggcgacttga gtaacgtctt cagcaataaa aactcatacc
     4201 gcgtcagcgt taatggcgtg tcaaaccagc tgatctgcgc cgcgggttca ttcaattcaa
     4261 aatgaccaat acggatgacg ggagacggcg acgagaactt cttcacccga cgcagtaagg
     4321 tgcgcaccct ggcgcacact tcgcggggtg aaaacggttt agccacatag tcgtcagcgc
     4381 caatttccag cccaagcagg cgatcgacct cttcacttcg ggccgtcagg aacagtacag
     4441 gtaacgccgg atggagcgcc agtaactggc ggcacaattc aaagccgctg atatcaggca
     4501 aaccaacatc gagaatcatg acgtcgggaa cctgctggcg agctttatcc agcaccggca
     4561 agcctcgctc aaagacctcg acggcaaaac cttcctgctg caacatgtag accagcgtgt
     4621 cggctatccc ttgctcatct tccactaacc agaccgtttc ccgttgcata aatcgcctct
     4681 gttattgccg ccacggcatg acaggaaccg cgctaatcgc gtttttcggc gaaccttcta
     4741 caactttgtc ggagtaagcc agataagcga gcgcgttgcg tttggcgtca taaaagcgca
     4801 cgacctgtaa cgacttaaag accagggacg tgcgtttttt gaatactacc tcgccctgag
     4861 ctttgccgtt tttaatacga tccgacagtt caatcggccc gacttgctga caagaaatag
     4921 ccgcatcgga ggtatcttcc gccagaccca atcccccttt aataccacct gttttcgccc
     4981 ggctcacata acaggtgaca tttttcacat cgggatcgtc aaaggcttcc acaacaattt
     5041 tgtgatccgg gccgatcatt ttaaatacgg tgtcgaccga accaatctct tctgcatgag
     5101 ccaatggccc cagcattatc aggcttaaag acaggatcaa atgcttgtat ttcatattgt
     5161 taccattttt atctattaca cgagcaatta gttcgtcacg gtaaataaca tattgattaa
     5221 gaacaaaatc tcaatacttt tatttccgtc aagccctaaa acatactcta ctaagaaaaa
     5281 aacactgaat gctaaaacag caaaaaatgc tattatccaa ttacctgatg tcaggtgctc
     5341 gttgttgaaa ggatgaggat attttatgga tcaggccggc attattcgcg accttttaat
     5401 ctggctggaa ggtcatctgg atcagcccct gtcgctcgac aatgtagcgg cgaaagcagg
     5461 ttattccaag tggcacttac agagaatgtt taaagatgtc actggccatg ctattggcgc
     5521 gtatattcgt gctcgtcgtt tgtcgaaatc ggcggtcgca ctacgcctga ctgcgcgtcc
     5581 gattctggac atcgcgctgc aataccgctt cgactctcaa cagacattta cccgcgcatt
     5641 caagaagcag tttgcccaga ctcctgcact ttaccgccgt tctcctgaat ggagcgcctt
     5701 tggtattcgc ccgccgctac gtctgggtga attcacaatg ccagagcaca aatttgtcac
     5761 cctggaagat acgccgctga ttggtgttac ccagagctac tcctgttcgc tggagcaaat
     5821 ctctgatttc cgccatgaaa tgcgttatca gttctggcac gattttctcg gcaacgcgcc
     5881 gaccattccg ccagtgctct acggcctgaa tgaaacgcgt ccgagtcagg ataaagacga
     5941 cgaacaagag gtattctata ccaccgcgtt agcccaggat caggcagatg gctatgtact
     6001 gacggggcat ccggtgatgc tgcagggcgg cgaatatgtg atgtttacct atgaaggtct
     6061 gggaaccggc gtgcaggagt ttatcctgac ggtatacgga acgtgcatgc caatgctcaa
     6121 cctgacgcgc cgtaaaggtc aggatattga gcgatactac ccggcagaag acgccaaagc
     6181 gggagatcgc ccaattaatc tgcgctgtga actgctgatt cctatccgcc gttaacgctg
     6241 cagctcatct aatgcagggg cgtccagatg cgagatgtcc cctgcggttt caacgaccca
     6301 gccagacgcc agccacaggc tttcctgata atccacgcgc gaaatagaac agttacgcag
     6361 acgtaagcgg cgctctgccc atgctggtaa tccgagaatc gtactcacca gacatcccag
     6421 tgcaatacca tggctcacca gcagcggtcg gcttccctgc ggtaagtccc ggcaagattc
     6481 cagtgccgca ttgacgcgat cgctgagctc ctgcattgac tcgccttcag gaatacgccc
     6541 gtcaacggtg ccattgacca gctgccgacg ccagttctct tcttcttcgg tcagagaatc
     6601 gatatgtctt ttttccagca cacccatgtt taattcacgc aggcgagaat caaagatgat
     6661 gtcacagccg caggcctggg cgatgatttc cgccgtacgc cgggtgcgtc ctaaatcgct
     6721 actgataata tgcgtaatgc caagctcttt ggcacgggtt gccacctgca tcgcctgttg
     6781 ctcacctttg gcggtcagcg ggctgtcaga ctggccctga atacgtcgct cggcgttcca
     6841 ctgcgtttca ccgtggcgga ctaggtatac ctgtaacatg ctttttttcc gtatactgct
     6901 gtaacgttaa ttttctgagt aatgctgatt atgcaccaag ttatctgtgc gaccaccaat
     6961 cccgctaaaa ttcaggccat tctgcaggca tttcacgaga tcttcggcga aggatcctgc
     7021 catattgcat ccgttgccgt cgagagcggt gtaccggaac agccctttgg cagtgaggaa
     7081 acgcgcgctg gcgcacgaaa tcgggtagca aatgcccgcc gtttacttcc agaggctgat
     7141 ttttgggtgg cgattgaagc tggcatcgat ggtgacagca ctttcagttg ggtggtgatt
     7201 gaaaacacca gccagcgcgg cgaggcgcgt tcggctactt tgccgttacc tgcggtgatt
     7261 ctgcagaaag tgcgtgaagg tgaggcgctt ggtcccgtga tgtcgcgtta taccggtatt
     7321 gatgagattg gtcgtaagga aggggcgatc ggcgtgttta ccgccgggaa actcactcgc
     7381 gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc cgtttcacaa tgccgtgtat
     7441 caggcattgt aggacggata aggcgttcac gccgcatccg gcattcggtg cacgatgcct
     7501 gatgcgacgc tgccgcgtct tatcaggcct acaaaatcaa tcgcttttca gcaacacctc
     7561 ttccagccac tggcgcagct ccacgggcgc ggctttcagg ctgttagatc cacgcgtaat
     7621 cgtcgcgatg cccgcgccga gttcattttt taactcacgc tgactcattt cgccacgcaa
     7681 cagctcttcg acaatacgca cgcgagtccc caacgcttcg cgctcatctg gcgtcagcat
     7741 caggtttaac aacggtaaat ggagatcgtt ttggtaggca ttcttaagca ggtcgacaaa
     7801 acgtaaccac tcctggtgac gctgttctgc catcgctgct gaatagggtg attgttgggc
     7861 cataatatgt cgccattgtt agcgggggaa gcaaaatgcc tcccccggtt gtactcgcta
     7921 aagagtacga tagcatatca taaacgtgcg gatcagtaac gacgtcccca ttccgtggcg
     7981 ctcatcaacg tcggtttatc ccccatgaaa taacggtagt aagcgtcata agccagcacg
     8041 ttcttcacat aaccgcgcgt ctcggagaac ggaatactct cgacaaatgc cacagcgtcg
     8101 atacgcccgg cgctgttgcc aagccaggtt cgcacccgtc caggtccggc gttataagct
     8161 gctgaggaga aaatacggtt attgccaaac tgctgataaa catattgcag gtaactggtg
     8221 ccaatgttga tattcgtttc cggatccagc aattgcccag ggctgctata accaggaata
     8281 gagaacatct tcaccgtatg ggtcgctgta ccaggcataa tctgcatcag gccgctggcc
     8341 cctaccggtg atttcacttt cggattccag gcgctctcct gacgggcaat cgccatcgca
     8401 tagctttgcg ggatctcttt cccactggtg taacgtttga aaagatcgtt gtaagccagc
     8461 gggaatcgct cttccagatg atcccaaagc ttcccggcga tcgttgcctg aacgctaaga
     8521 tcccaccatt ggttattgaa agcataccga gccagttgag cctgctctgt ttttgacttg
     8581 ctcttcacca gattggccca ctcgctacgc gcggtgttat cgagattcca gtacatcaac
     8641 tcgcgcacgc gcgccatctc cggcccctga gtcagggcgc tgtcaacatt ctgcggcgct
     8701 ttatcaatct tcagctcata ctcttctccg atgcgttgtg ctgcaaccat cgggtagaaa
     8761 ccacgctgtt gcatgagttg atgcaaaatc tctttcgctt cagcttcacg tccgcgttcc
     8821 agcaataaat ccacctgcca gtaacgccat tcatctttct cttttgcttc catcggcaga
     8881 cgcgccagcc aggtattcag gccgcgacga tcgccggtgc caagtgccat tcgcacacgg
     8941 cgttcaatca acgacgtgga ttgcgagcgc ataatgacgt catcgcgcca tttcgcctgc
     9001 tcgtcggtga catcgttgcc catcaaacgc caggcgacga tatcgcgcag ctcctgaatc
     9061 tgatcttcat taagctgctg cacctgggca agcgacggga tcatcagccg tgcattttca
     9121 gcatcctgcc gcgccacact ggcaaacgcc accgctgcca tttgacgggt aaaatcggtc
     9181 gctccggttg tacgcgcgaa ggtcagtacc gtattagggt tgttcgccag tgaaatgatt
     9241 gccgaggcga tagtctggta atcggcaggc atctgccctg ccagcactgt taccaggcca
     9301 gtgttacccg ctttcatcgc cagacggata cgctctaaat acgccagcgg atcttgttta
     9361 cctgacgcac gccagacgct aaataacttg tcacaggcgt taggctggct cttgccggtt
     9421 agccacagct ctttcgcccc ttgccaggct tcttcactct gcccggtgtt ccatttcgca
     9481 tagtagtaat tacattgcgc ttcggtagtt ccgggctttt ccgggctaaa ggctaacaag
     9541 ccacgccagt cttcacgccg cgccagttca ttgacgaaac gagattgcag cgtgcgagcg
     9601 ggaggaagcg tggggttagc gcgaacaaag ttagtgaccg tcaccgccgg ttgattcatc
     9661 agatcatcgg tgatctggcg gtattccagg tagggataaa gcggataatc cttcagtcca
     9721 ggcatcattt gttcgaccac atccatttgt cgattatccc aggcctgctt gatttgcgcg
     9781 taacggctac gctgctcatc cagtgagtcg gctcgcgcca cgctgctgac cgtcagcaga
     9841 cagacaccgg cagccaacag ccgccaggta acttgtttgg ctttttccac aagcgcatcc
     9901 tctaagtgta aatacatcaa tgcagcgtca tgccgcgtaa tagatttatg ctaaccagtc
     9961 attgccgttt acgccacgtt acggacactt ttttactttt actgcgaagg tgatcgacca
    10021 actgggatta ggcggggaaa aaggctacac ttcgcctttg aaaactcatc atcacgataa
    10081 acatagaggc gaagtccaac gtggctcaat tcgtttatac catgcatcgt gtcggcaaag
    10141 ttgttccgcc gaaacgtcat attttgaaaa acatctctct gagtttcttc cctggggcaa
    10201 aaattggtgt cctgggtctg aacggcgcgg gtaagtctac cctgctgcgc attatggcgg
    10261 gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc agacatcaag attggttacc
    10321 tgccgcagga accgcagctg aacccggaac acaccgtgcg tgagtccatt gaagaagcgg
    10381 tttctgaagt ggttaacgcc ctgaaacgcc tggatgaagt gtatgcgctg tacgccgatc
    10441 cggatgccga ttttgacaag ctggccgctg aacaaggccg tctggaagag atcattcagg
    10501 ctcacgacgg tcataacctg aacgtacagc tggagcgtgc ggcggatgcg ctacgtctgc
    10561 cggactggga cgcgaaaatc gctaacctct ccggtggtga gcgtcgtcgc gtagcgttgt
    10621 gccgcctgct gctggaaaaa ccagacatgc tgctgctcga cgaaccgacc aaccacctgg
    10681 atgccgaatc cgtggcctgg ctggaacgct tcctgcacga cttcgagggc accgtggtgg
    10741 cgattaccca cgaccgttac ttcctcgata acgttgcagg ctggatcctc gaacttgacc
    10801 gcggtgaagg tattccgtgg gaaggcaact actcctcctg gctggagcag aaagatcagc
    10861 gcctggcgca ggaagcttca caagaagcgg cgcgtcgtaa gtcgatcgag aaagagctgg
    10921 agtgggtacg tcagggaact aaaggccgcc agtcgaaagg taaagcacgt ctggcacgct
    10981 ttgaagagct gaacagcacc gaatatcaga aacgtaacga aaccaacgaa ctgtttattc
    11041 cacctggacc gcgtctgggc gataaagtgc tggaagtcag caacctgcgt aaatcctacg
    11101 gtgatcgcct gctgattgat gacctgagct tctcgatccc gaaaggggca atcgtcggga
    11161 tcatcggtcc gaacggcgcg ggtaaatcga ccctgttccg tatgatctct ggtcaggaac
    11221 agccggacag cggcaccatc actttaggtg aaacggtgaa actggcgtcg gttgatcagt
    11281 tccgtgactc aatggataac agcaaaaccg tttgggaaga agtttccggc gggctggata
    11341 ttatgaagat cggcaacacc gagatgccaa gccgcgccta cgttggccgc tttaacttta
    11401 aaggggttga tcagggtaaa cgcgttggtg aactttccgg tggtgagcgc ggtcgtctgc
    11461 atctggcgaa gctgctgcag gttggcggca acatgctgct gctcgacgaa ccaaccaacg
    11521 acctggatat cgaaaccctg cgcgcgctgg aaaacgccct gctggagttc ccgggctgtg
    11581 cgatggttat ctcgcacgac cgttggttcc tcgaccgtat cgccacgcac atcctggact
    11641 accaggatga aggtaaagtt gagttcttcg aaggtaactt tactgagtac gaagagtaca
    11701 agaaacgcac gctgggcgca gacgcactgg agccgaagcg tatcaagtac aagcgtattg
    11761 cgaagtaatg cgtaaaatgc cggatgcggc gtgaacgcct tatccggcct acaaaccaag
    11821 aaatttcaat acgttgtgaa tctccaggcc tgataagacg cggtaagcgt cgcatcaggc
    11881 attgagcacc gaatgccgga tacggcgtga acgccttttc cggcctacaa cagccttagt
    11941 atccgcaatt tcgtagcggg tcacccgtgc aggttttttc aaaccttgtt taatctcgac
    12001 agcctcttcc agagaggttt tcaactcatc aaaaaactta ttttatacct cgcctttgag
    12061 ccgtttcatc gcggttatct ctgctccccc atcatctccc gcaccagctc cacgcagcgc
    12121 aggaaacggc tgtcataatc gtcctcttca acccgcacga attcgatatt gttctcttcc
    12181 agcatctcca ccagcaagtt ctggaactct ttgcgatcca ccgaactgcc gaggctgcgt
    12241 aaaccatccg ccacccacgg cgtgttgttc tccagcagga tcaccagatc gaaacggtat
    12301 tcatcaatca acgcctgtac gaacggatgc tcacgccctt cgtacttttt gcagaacgcc
    12361 tgggtagtga caaaatcagt gtcaataaac gccactttat tggcatattt cactgcaaaa
    12421 tcaatgtatt gtgcgtggcc cagcgcgatt ttatcgtaat cggaatactg caatgcgatc
    12481 tcatcaccgc cgaggtgtga aaagacataa tcgcgaccat attcccacgc actggtggta
    12541 ttgaagatat tggcaagttt gtttaccagg gtggatttac cgctcgactc gccaccaagg
    12601 atcgccacgg tacgtacaaa gaacggcttc acttccgtag gaatatattc ccagtagcgg
    12661 aacgggtttt cgcggatctg cgcaccgctg atactcataa aggtacgttt cggatcgacc
    12721 agcaccgtat cgatccccag atgttccata tactgtggcg catcggcttc ttccgaggtg
    12781 tagatcaggt ccggctgaat ccccttttca gccataaact ttttgatgcc gttgctccac
    12841 acatcccagc cgtgcggata cggctccatg ccctcttcgt tgaaagcatg aatgcgaata
    12901 tttttctgat atttaaaagt ttgtaataac cagcgcagac gatccggcac cgtgggctgc
    12961 tgcgacatgg cgctgtcttc aaacaacgcg cggtcgcggg tatcgtcaaa acccataatg
    13021 atatgcagct cgtcaacctg gctacaggcg cgctggataa ggtagatatg tccggtatgc
    13081 agcgggtaga acttaccgaa tacgacacca atggttttct tctgtcgggg aaattcaagc
    13141 cccaaaaaac ggtgcaacgc ctccagcttt tgcgcgctgg ggcttttaat tttggcattc
    13201 agtaactggc ttaaataccc tttggtcata ccgctggcat cagctacctg ctgtagcgtg
    13261 cagccctgtt gcttgatggc agttttcagg taatcaaatg acgacatatc tccctccgta
    13321 tctctcatta taagtcgtcg aacacgctaa gcgcgtcgga gagtttttta acgccaaaaa
    13381 tctgcatccc ttccggcgct tttttcggta cgttagccgc cggaacaatc gcccggcgaa
    13441 aaccgtgttt cgccgcttct gaaattcgtt cctgaccgct gggcaccggg cggatctccc
    13501 ctgccagccc gacttcacca aacaccacca gatcctgtgg cagcggtctg tcacgcaggc
    13561 tggaaaccat cgccagcagt aacgctaaat cggcactggt ttcggttacc ttcacgccgc
    13621 cgaccacgtt cacaaataca tcctgatcgg ccatttgcag accaccgtga cggtgcaaga
    13681 cagccagcag gattgccaga cggttttgtt ccagccccac tgccacgcgg cgcggattcg
    13741 ccatcatcga gtgatcgacc agcgcctgaa tctccaccag caacggacgc gttccttccc
    13801 acaccaccat cacggagctg ccggaggtca cttcatctcc gcgacttaag aaaattgccg
    13861 aagggttgct gacttcacgc agcccctgtt cggtcatcgc gaagacgccc agctcattca
    13921 ccgcgccgaa gcggttttta tggctgcgca aggtgcgaaa acgggagtcg gcatcaccat
    13981 ccagaagcac cgaacagtcg atacagtgtt ccagcacttt agggccagcc agcgagccat
    14041 ctttggttac gtgcccaacc atgacaatcg ccacaccgcg cgttttggcg aagcgcgtca
    14101 gataagccgc cgtttcacgc acctgcgcca cgctaccagg cgatgattgt acatccgcca
    14161 tatgcatcac ctggatcgag tcaattacca tcagtttcgg ttgctcttct tcggcaatca
    14221 ggcagatctg ttcgatgctg gtttccgaca acatattgag attgtcagtc ggcaggccaa
    14281 ggcgatgagc gcgcattgcc acctgttgca gcgactcttc gccagtgaca tacagcgttt
    14341 tcatctgctg ggccagtttg cacagcgttt gcagtagcag cgtggatttc cccgcacctg
    14401 ggttaccgcc aatcagaatg gcgcttcccg gcaccacgcc gccgcctagt acgcggtcga
    14461 actctttaaa tccggtggaa aaacgcggca gctcttcaag gctgatatcg gagagtttct
    14521 ggacttttgc caccccggcg ctaccggcat agccgctgag acgctcgtta cgcgccaccg
    14581 ttggcgacgc agcaagacgc acctcggtga tggtgttcca ggcatgacag gcactgcact
    14641 gcccctgcca gcgcggataa tcggccccgc attcattaca aacaaaggcg cgttttggag
    14701 cttttgccac ggtgtacctc gttaatgctg tgccgcccgc aggatggcgg gcgagcaatt
    14761 acttctgatt caggctgcca gagaggatgc agaatacccc catcaggtca gcgtgacgga
    14821 tggtgacttc cgccttttca ttcacttttg gcttggcatg gtaggcaatc cccagccctg
    14881 ctgctttgat catcggcagg tcattagctc catcgccaat cgccacggtc tgcgccagcg
    14941 ggatttcata ctcctgcgcg aggcgagtca aagttttcgc tttgtactgc gcgtctacga
    15001 tgtcgccgat cacattgccg gtaaatttac cgtccatgat ctccagttca ttggctacca
    15061 cggcggtcag gcgcagcttg tcgcgcagat attcagcaaa gaaagtaaag ccgccggagg
    15121 caatcgccac tttccagccc agcgtttcca gcttgagcac cagttgcgtt aagcctggca
    15181 tcagcggcag attttcacgc acctgttgca gaatattggc gtcagcgcct ttcagcgtcg
    15241 ccacacggct gcgcaggctg gcggtaaaat cgagttcgcc gcgcatcgcc cgttcggtta
    15301 cttccgccac catctcgccc gttccggcca gtttggcaat ttcatcaata cattcaatct
    15361 ggatggcggt ggaatccatg tccatcacca gcaaacccgg cgtgcgcagg tgcgggattt
    15421 tccccagcgg ggcgacatcc agttgcgctt catgggccag gcgcgtagcc cgtgcggtga
    15481 gtgaacctgc cagacgaatc acctgataat cttccacgca ccaggcggca acaatcacca
    15541 tcgccgcacc cagtttgctc tggtattggg tcagacgctg tttatccagc ccacgaccat
    15601 acagcagcca gccgctacga cctgcgtggt aatccagtgg catcacttca tcaccactta
    15661 atgaaagagg cagacccggc cataaagaga catcttcagg caggtcgcac caggtaatgt
    15721 taggcattaa ggctcctgta aaatcgttcg aagcagggaa aataacgcat gaggctacct
    15781 tgtatccatt gcttctggca acattaagtc tcaaattttc aaagggtgga agatggctcg
    15841 cacaaaactg aaattccggc tgcatcgggc agtgattgtc ctgttctgtc ttgccttgtt
    15901 agtggcactg atgcaggggg cgtcatggtt tagtcaaaac caccagcgac agcgtaatcc
    15961 acagctggaa gaactggccc gcaccctggc gcgtcaggtg acgctgaacg ttgcaccgct
    16021 gatgcgtacc gactcaccag atgaaaaacg cattcaggcg atcctcgatc agttaacgga
    16081 tgaaagccgc atcctcgacg cgggtgtgta tgacgaacaa ggcgatctta tcgcccgttc
    16141 aggcgaaagc gtcgaagtgc gcgaccggct ggcgctcgac ggtaaaaaag caggcggcta
    16201 ttttaaccag cagattgtcg agccaattgc gggtaaaaac ggaccgctcg gctatctgcg
    16261 cctgacactc gacacccata cgctcgccac cgaagcccaa caggtggata acaccactaa
    16321 cattttacgc ctgatgttgc tgctctcact ggcaatcggc gtagtgctga cccgcacgct
    16381 gctacagggt aaacgcaccc gctggcagca atcgcccttc ctgttaaccg ccagcaaacc
    16441 ggtgccggaa gaggaagaaa gcgagaaaaa agagtgaccc attactacaa gaaaggaaat
    16501 cgttatgtcc acattacgcc tgctcatctc tgactcttac gacccgtggt ttaacctggc
    16561 ggtggaagag tgtatttttc gccaaatgcc cgccacgcag cgcgttctgt ttctctggcg
    16621 caatgccgac acggtagtga ttggtcgcgc gcagaacccg tggaaagagt gtaatacccg
    16681 gcggatggaa gaagataacg tccgcctggc gcggcgcagt agcggtggcg gcgcggtgtt
    16741 ccacgatctc ggcaatacct gctttacctt tatggctggc aagccggagt acgataaaac
    16801 catctctacg tcgattgtgc tcaatgcgct gaacgcgctc ggcgtcagcg ccgaagcgtc
    16861 cggacgtaac gatctggtgg tgaaaacagt cgaaggcgac cgcaaagtct caggctcggc
    16921 ctatcgcgaa accaaagatc gtggcttcca ccacggcact ttgctgctca atgccgacct
    16981 tagccgcctg gcaaactatc tcaatccgga taaaaagaaa ctggcggcga aaggcattac
    17041 ctcagtgcgt tcccgcgtga ccaacctcac cgagctgctg ccggggatca cccatgagca
    17101 ggtttgcgag gccataacca aggccttttt cgcccattat ggcgagcgtg tagaagcgga
    17161 aatcatctcc ccggacaaaa cgccagactt gccaaacttc gccgaaacct ttgcccgtca
    17221 gagtagctgg gaatggaact tcggtcaggc tccggcattc tcgcatctgc tggatgaacg
    17281 ctttagctgg ggcggcgtgg aactgcattt cgacgttgaa aaaggccata tcacccgcgc
    17341 ccaggtgttt accgacagcc tcaaccccgc gccgctggaa gccctcgccg ggcgactgca
    17401 aggctgcctg taccgcgcgg atatgctgca acaagagtgc gaagcgctgt tggttgactt
    17461 cccggaccag gaaaaagagc tacgggagtt gtcgacgtgg atagcggggg cggtaaggta
    17521 actcgctgat taaaatacca aaggaaggga atatgatcga tctggaaaac caggagcggg
    17581 agattatcaa tttaatgtta agccagcgta tttcctggct agccgccgtg cgtatcaggc
    17641 ataaattatc gcttgctgaa gtaagtaaaa tgttaggtat ctcgattaat tctctaaaat
    17701 agatcgaaaa gacggaacgg cttagctcaa acatcaaaag caaaatggga gaaatttatg
    17761 gttgtccacc agagttgctg atatgccctt cctggatgac ggctgaacat aaatgaatag
    17821 attcgtgaat agatacttga atgtatttta ttttttatcc atatgaaaaa taatagtaaa
    17881 tctataaatc agttgctatt gatgaataga tactaaaaag ccggagcagt cccccactcc
    17941 ggcttaacac gacaatcgcc tttcagcgaa acacaattac tctttatcgc ccagcagaac
    18001 ggattccagt gcgattttga tcatgtcgtt gaaggtggtc tgacgctcag cggcagtggt
    18061 ctgctcgtga gtgcggatgt ggtcagacac agtgcagatg gtcagggctt tcgcgccaaa
    18121 ttctgcagcg acgccgtaga taccagccgc ttccatttcc acgccgagga tgccgtattt
    18181 ttccatcacg tcgaacattt cgccgtccgg agagtagaac aggtcagcgg agaacaggtt
    18241 accaacgcga gcatcaatgc ccagtgcttt agccgcgtct accgcgttac gcaccatgtc
    18301 aaagtcagcg atagcggcaa agtcatgatc tttaaaacgg atgcggttaa ctttggaatc
    18361 ggtgcaggca cccataccga taacgacgtc gcgcagtttt acgtgcggca gaactgcgcc
    18421 acaggaaccc acgcggataa ttttcttcac gccgaaatcg gtgatcagtt ctttggtgta
    18481 gatggagcag gacgggatac ccataccgtg acccattacg gaaattttgc ggcctttgta
    18541 agtaccggtg aagcccagca taccgcgaac gttgttcact tcacgggcat cttcaaggaa
    18601 agtttcagca atatacttcg cacgcagcgg gtcgcctggc atcaaaacta cgtcagcgaa
    18661 atcgcccatt tctgcattaa tgtgtggggt agccattgtt ttatccttat caacatgttt
    18721 tcaccctctc cctgagggag agggccgggg tgagggcatc aagccgcacc cattatcaga
    18781 acatggcttt gccatattcc atatcagaag taccaaaata ttttgccaga gtctggccga
    18841 tatccgcgaa ggtttcacgg tgacccagtg agcccggttt tactttcggg ccgtaaacca
    18901 gtaccggaat gtgttcacgc gtgtggtcag taccggtcca ggtcggatca caaccgtggt
    18961 cagcagtgag gatcaggatg tcgtcatcgc gcagcagaga catcagctcc ggcagacggc
    19021 ggtcgaacag ctccagaccc acagcataac cggcgacgtc gcgacggtgg ccccaggaag
    19081 agtcgaagtc aacgaagttg gtgaagacga tggtgttatc acccgcttct ttcatctctt
    19141 tgatggtggc gtcaaacagc gcgtccaggc cagttgcttt cactttcttg gtgataccgc
    19201 agttggcgta gatgtctgca attttaccga cagaaaccac ctgaccgtgt ttttcatcaa
    19261 ccagtttctg cagcacggtc ggtgctggcg gctcaacagc caggtcgtga cggttaccgg
    19321 tacgctggaa gttaccggct ttgtcaccga taaacggacg agcgataaca cgaccgatgt
    19381 tgtagccgcc gttggtcagc tcttcacggg cgatttcgca cagttcgtag agtttatcca
    19441 ggccaaaggt ttcttcgtgg caggcaatct ggaacacaga gtcagcggag gtatagaaaa
    19501 tcggcttgcc ggttttcatg tgctcttcgc ccagctgatc cagaatgacc gtaccggaag
    19561 agtggcagtt accgaggtaa cccggcagat tagcgcgttc aaccagttta tccagcagct
    19621 cttgcgggaa gctgttttcg tgatcggaga aatatcccca ctcaaataga accgggacac
    19681 cggcgatttc ccagtgacca gacggggtat ctttaccgga tgacatttcg tgcgcccatg
    19741 cgtacgcgcc gataacttca gcgttgccgt ccattcccgc cggaatgaaa ccggtagaac
    19801 cttcgtgtgc tttcgccagc cccagacggg tcagatttgg cagattgagc ggacctttac
    19861 gaccgttatc agcttcgcct ttggcacaag cttctgcgat atgacccagg gtgtcagccc
    19921 cgacgtcacc aaagcgttct gcatcttctg tagcgccgat gccgaatgag tccagcacca
    19981 taataaatgc acgtttcata tgttctccgt actcagtgct tcaaatttaa atgatcagat
    20041 cagtataccg ttattcgctg atacggcgat agacagttgg tgtgctttcc ggtgctttat
    20101 cggcaagttt aattgccgct ttcaccgctt tcgccgcttc ctgccagctg ttttcgtctt
    20161 tcgcgtggat aaccgccagc ggacgctgac cgtctacctg gtcgcccaaa cgcgccatat
    20221 cagtaaagcc gacgctgtaa tcaatggtgt cagatgcctg acgacgaccg ccgcccattg
    20281 caaccactgc catccccagc gcgcgggtat ccatttcact gacaaaacct tcggtatcag
    20341 catagactgc tttcgtcagc atcgctgtcg gcagatactt cgcgtagttc tcaacgaagt
    20401 cggttgggcc tttttgtgcc gctaccatac gaccaaagac ttctgctgct ttaccgttgt
    20461 ccagcactgc ttgcaatttc gcgcgcgctt cggcgtcatc tttcgccagt ttgccggaga
    20521 taagcatctc cacgcacagc gccatcgtga catcaaacag acgcgggtta cgatactcac
    20581 ccgtcaggaa ctgcaccgct tcacgaactt caaccgcgtt acctgcactg gaggccagta
    20641 cctgattcat gtcggtgagc agcgcggtgg tgcgcacgcc agcgccgtta gccacgccaa
    20701 caatcgcttc ggcaagggct tcagagagtt cgtaggtcgg cataaacgcg ccgctaccca
    20761 ctttcacgtc catcaccagc gcgtccagac cttccgcaag tttcttcgcc agaatagagg
    20821 cggtgatcag cgggatggag tccacggttg cggtaatatc acgggtcgcg tagaaacgtt
    20881 tatccgccgg agccagtgag ctggtctgac cgataatcgc cacgccgacg tctttaataa
    20941 tttcgcggaa acggttgtca tccgggaaaa tgtcgaagcc agggatggat tccagtttgt
    21001 cgagcgtacc gccagtatga ccgaggccgc gaccagagat catcggaata tagccgccgc
    21061 aggctgcgac catcggcccc aacatcagcg aagtcacatc gccgacgcca ccagtggaat
    21121 gtttatcaac aatcgggcca ttcagatgca ggcttttcca gtcgagaacg gttcctgaat
    21181 ctcgcatcgc catggtcagc gagacacgct caggcattgt catatcgtgg aagaaaatgg
    21241 tcatcgcgag ggcggcaatc tgcccttcgg agatagtgtt gtcgcgaatg ccgttgataa
    21301 agaaacgaat ttcttcatcg ctcagtgcat gaccatcacg ttttttacga ataatttctt
    21361 gtgcgagaaa caaggtaacc tcccgaggaa aagagtatga ggcggctttt gccggatgcg
    21421 tctacgcctt atccggccta cgggtagata cgtctcgtag gcctgataag cgcagcgcat
    21481 caggcgtaaa gcatcttact tagtagctgc tggcgctctt accgtcaccg tggcccagcg
    21541 ctttcaacag gcttgccagc aggctggaag caccaaagcg gtagtgacgc gcatctgccc
    21601 agtcagcacc gaacagttca tctgcgatgg cgagatattt ctgcgcatct tccgcagtac
    21661 gcacgccgcc cgctggtttg aaaccaacgg ttttttctac gcccatatca cggatcactt
    21721 ccatcatgat gcgcgcgctt tccggcgtcg cgttcacagc cactttaccg gtagaggttt
    21781 tgatgaagtc cgcacccgct ttgatggaga tttcagacgc tttacggatc agcgcttcgt
    21841 ctttcagttc gccggtttcg atgatcactt tcagcagtac attcgctgcc gcgcaagcct
    21901 ctttacaggc tttcaccagg tcaaaaccaa cctgctcgtt acccgccatc agcgcgcggt
    21961 acgggaacac aacgtcaact tcatcagcac cgtaggcgat tgccgcacgg gtttctgcca
    22021 gcgcgatttc gatgtcatcg ttaccgtgtg ggaagttggt taccgtagcg attcggattt
    22081 ccggggtgcc ctgctctttc agcgttttgc gagcaatcgg gataaagcga ggatagatac
    22141 agatagcggc ggtattgccg accggggttt tggcctgatg acacagagca attactttct
    22201 cgtcggtgtc gtcgtcattc agggtggtca ggtccatcaa tttcagtgca cgcaggctgc
    22261 ttgctttcag atcagtcatt tcattctcct ggcttgtcgc caataaaatt caccttgcga
    22321 gtttgttagt attctaacat ctactccgca acacacttcg atatacatca caattaagga
    22381 aatctactta caagtttgca tcactgtaat gcgatctggt tcaaataatt cactttcaaa
    22441 tgaatgcgtc agtggtggca aacgcatcag gatcaaagtg aacatcacga aacttcttac
    22501 aatggcgcat caccttcggc ataaaaggaa aagaagatgc ccacttctca tgaaaatgca
    22561 ctgcaacaac gttgccagca aattgtcacc agcccagtgc ttagcccgga gcagaagcgc
    22621 cattttctgg cactggaagc agaaaacaat ctgccttatc cgcagcttcc tgccgaagcc
    22681 cgccgcgcgc tggatgaggg tgtaatctgc gatatgtttg aaggtcatgc gccgtacaaa
    22741 ccgcgctatg tcttacccga ttacgcccgt tttctggcga acggttccga atggctggag
    22801 ctggaaggcg cgaaagatct tgatgacgca ctctctctgc tgaccattct ttaccaccac
    22861 gtaccgtcgg tcacatcgat gccggtctac ctggggcaac tggatgcgtt gttgcaacca
    22921 tatgttagaa ttctaacaca agacgagatc gatgttcgaa taaaacgttt ctggcgttac
    22981 ctcgacagaa ccctgccaga cgcctttatg cacgccaata tcggcccgtc tgattcgccc
    23041 attacccgcg cgatcttacg tgcagacgca gagttgaagc aggtttcacc gaacctgacc
    23101 tttatctacg atcctgaaat cacccctgat gacctgctgc tggaagtggc gaagaacatc
    23161 tgtgaatgta gcaaaccgca catcgccaac ggtccggtgc atgataaaat tttcacaaaa
    23221 gggggctacg ggattgtgag ctgttacaac tcactgccgc tggcgggtgg tggcagcacg
    23281 ctggtacgcc ttaacctgaa agccattgcc gagcgcagcg aatcgctgga tgacttcttt
    23341 acgcgcactc taccgcacta ctgccagcag cagatcgcca tcatcgatgc gcggtgtgaa
    23401 ttcctctatc aacaatcaca cttctttgag aatagcttcc tggtgaaaga agggctgatt
    23461 aaccctgaac gttttgtgcc aatgtttggc atgtatgggc tggcggaagc ggttaacttg
    23521 ctgtgtaaga aagaagggat tgccgcgcgc tacggtaaag aagccgccgc aaatgaagtg
    23581 ggttatcgca tcagcgcgca actggcggag tttgtcgcca atacccccgt gaaatatggc
    23641 tggcaaaaac gcgccatgtt acacgcacag tcggggatca gttccgatat cggcaccacg
    23701 ccgggcgcgc gtttgccgta tggtgatgag ccagatccga tcacccatct gcaaactgtc
    23761 gccccgcatc atgcttatta ttattccggc atcagcgaca ttctgacgct cgacgaaacc
    23821 atcaaacgta atccgcaggc gctggtacag ctttgcctcg gtgcctttaa agccggaatg
    23881 cgtgaattta ctgccaatgt cagcggtaac gatctggttc gcgttaccgg ttatatggtg
    23941 cgtttgtcgg atttagaaaa atatcgcgcc gaaggttcac gcaccaacac cacctggctg
    24001 ggcgaagaag ccgcacgcaa cactcgtatt ctggaacgcc agccgcgcgt gataagccat
    24061 gaacagcaga tgcgctttag tcagtaagat tatccccttc tcctgcgttg acgggccagg
    24121 cagtcgtctg gctctgtttt tgcagggctg caatctgcgc tgcaaaaact gtcacaatcc
    24181 gtggacgatg ggacgttgca atgactgtgg ggagtgcgtg ccacagtgtc cgcatcaggc
    24241 gttgcagatt gttgacggca aagtggtgtg gaacgctgtg gtttgcgagc agtgtgatac
    24301 ctgcctgaag aggtgtccgc aacatgccac gccaatggcg caatccatga gcgtggacga
    24361 agtgcttagc catgtccgca aagcggtgct gtttattgaa gggataacgg taagcggcgg
    24421 cgaagccacg acccaactgc cgtttgtagt ggcgctgttt actgctatca aaaacgatcc
    24481 gcaactgcgc cacctcacct gcctggtgga cagtaacggc atgttgagcg aaaccggctg
    24541 ggaaaaatta ctcccggtgt gtgacggcgc aatgctcgat ctcaaagcgt gggggagcga
    24601 atgtcatcaa cacttaaccg gacgcgataa tcagcagatt aagcgcagca tctgtttgct
    24661 tgcagagcgc ggcaagctgg cggaactgcg tttgctggta attccagacc aggtggatta
    24721 tttgcatcac atcgatgagc tggcgacgtt tatcaagaga cttggcgatg ttccggttcg
    24781 cctgaatgcg tttcatgccc acggcgtgta tggcgaggcg caaagctggg cgagcgccac
    24841 gccggaagac gttgagccgt tggctgatgc gttaaaggtg cgcggggtga gccggttgat
    24901 atttccggcg ctctatttgt gacatgccgg atacggccta cggttcggca cagacttgta
    24961 ggcatgataa gacgcgacaa gcgtcgcatc aggcattatg cgccgactgc cggatgcggc
    25021 gtgaacgcct tatccggcct acggttcggc acagacttgt aggcatgata agacgcgtca
    25081 gcgtcgcatc aggcattgtg cgccaactgc cggatgcggc gtgaacgcct tatccggcct
    25141 acggttcggc acagac
//
LOCUS       DAZRUF010000037        25106 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZRUF010000037 DAZRUF010000000
VERSION     DAZRUF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.037"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            196..384
                     /gene="bcsR"
                     /locus_tag="ACHXOL_001229"
     CDS             196..384
                     /gene="bcsR"
                     /locus_tag="ACHXOL_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR6808256.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHXOL_001230"
     CDS             396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHXOL_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR6808257.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1145..1309
                     /locus_tag="ACHXOL_001231"
     CDS             1145..1309
                     /locus_tag="ACHXOL_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR6808258.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHXOL_001232"
                     /pseudo
     CDS             1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHXOL_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHXOL_001233"
     CDS             3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHXOL_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR6808259.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVLRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHXOL_001234"
     CDS             6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHXOL_001234"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR6808260.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHXOL_001235"
                     /pseudo
     CDS             7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHXOL_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHXOL_001236"
     CDS             10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHXOL_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR6808261.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12953..14239
                     /gene="dctA"
                     /locus_tag="ACHXOL_001237"
     CDS             12953..14239
                     /gene="dctA"
                     /locus_tag="ACHXOL_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR6808262.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14459..15955
                     /locus_tag="ACHXOL_001238"
     CDS             14459..15955
                     /locus_tag="ACHXOL_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR6808263.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHXOL_001239"
     CDS             complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHXOL_001239"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR6808264.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHXOL_001240"
     CDS             17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHXOL_001240"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR6808265.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18049..20109
                     /locus_tag="ACHXOL_001241"
     CDS             18049..20109
                     /locus_tag="ACHXOL_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR6808266.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20291..21613)
                     /locus_tag="ACHXOL_001242"
     CDS             complement(20291..21613)
                     /locus_tag="ACHXOL_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6808267.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASIAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="ACHXOL_001243"
     CDS             complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="ACHXOL_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR6808268.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23086..23985)
                     /locus_tag="ACHXOL_001244"
     CDS             complement(23086..23985)
                     /locus_tag="ACHXOL_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR6808269.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24505..>25106
                     /locus_tag="ACHXOL_001245"
     CDS             24505..>25106
                     /locus_tag="ACHXOL_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR6808270.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5895 a         6363 c         6992 g         5856 t
ORIGIN      
        1 atttagcttg tgaatcaatt tagaaggtca caagacgtgt gattttagtc ttttcgtttt
       61 taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa
      121 acaataaacc ggcgaaatgc tcacggttag aattgtttca tgaacggtac ggttatttca
      181 tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc cgcgataggc
      241 tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc tgatattgat
      301 tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg gccgttgctg
      361 gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga ttgcaggggg
      421 tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca ttacaaatgt
      481 tgggagaaaa tgtcctggtg gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta
      541 atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc caggactggc
      601 gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt cagttatcca
      661 ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt tgctccggct
      721 tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta ccgcgtgatg
      781 cctcgcagat aacccaccag ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg
      841 tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc gcacatattt
      901 tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag ctttggttgc
      961 aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc
     1021 tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc gctggcggct gaagagatac
     1081 tgacgctggc gaactggtgc ctgttgaact actccgggct gaaaacgcca gtcgggagtg
     1141 catcatgagt atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg
     1201 gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt
     1261 ctggatgatc ctggcctgga tttttattcc gctggagcac ccgcgctagc agcgtattcg
     1321 cgcagaacat aaaaacctgt atccgcatat caacgcctcg cgtccgcgtc cgctggaccc
     1381 ggtccgttat ctcattcaaa catgctggtt actgatcggt acatcgcgca aagaaacgcc
     1441 gaaaccgcgc aggcgggcat tttcaggtct gcagaatatt cgtggacgtt accatcaata
     1501 gatgaacgag ctgcctgagc gcgttagcca taaaacacag catcttgatg agaaaaaaga
     1561 gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc
     1621 gctgattctg gcgttaatct gcgttactca accgtttaac ccgctggcgc agtttatctt
     1681 cctgatgctg ctgtggggtg tagcgctgat cgtacggcgg atgccggggc gcttctcggc
     1741 gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt ggcgttacac
     1801 ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc tgctcttcgc
     1861 tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat ggccgctgaa
     1921 tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt
     1981 cccgacttac aacgaaaatc tcaatgtggt gaaaaatacc atttacgcct cgctgggtat
     2041 cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca gggaagagtt
     2101 tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc atgaacatgc
     2161 gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt
     2221 cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa
     2281 agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg acccgtttga
     2341 acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct atggtctggt
     2401 gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctatg cggtgattcg
     2461 tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg aagatgcgca
     2521 tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc
     2581 ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg
     2641 cgggatggta caaatcttcc gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc
     2701 tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc cacggctgat
     2761 cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct atgcgccagc
     2821 gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga ccaactcgaa
     2881 gatccagggc aaatatcgcc actctttttg gagtgaaatc tacgaaacgg tgctggcgtg
     2941 gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca aatttaacgt
     3001 caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct cgcggcccta
     3061 catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc gctacttcta
     3121 tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct
     3181 gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca
     3241 ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg
     3301 taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc aggcgcagat
     3361 tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat acgtcttccc
     3421 gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc cgctcaccac
     3481 ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat gggcgctctg
     3541 gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc tgaagctcgg
     3601 cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg gcatattccg
     3661 tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccacc cggagcggag
     3721 cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa
     3781 gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc tctttcatga
     3841 cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta gccgcccaga
     3901 cggaacaaaa tccgcaggtg gggcaagtga tgccgggcgt gcagggcgct gatgcgccag
     3961 tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc
     4021 cgccgggcag catggtgcta cgtggcatta acccgaacgg cagcattgag tttggtatgc
     4081 gcagcgatga agtggtgacg aaggcgatgc tcaacctcga atacacgcca tcgccatcgt
     4141 tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc
     4201 cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt aacccactgt
     4261 ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg
     4321 aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga ctggatctga
     4381 cctatcagac cctgaatgtg aagagtgacc tgtcacactt cccggtgcca ttctttgacc
     4441 cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg gatgttgggc
     4501 tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc
     4561 agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc tttgcaacca
     4621 acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg gtgattgaga
     4681 tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt cgtgacgaca
     4741 aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa
     4801 gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact
     4861 gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa gaacagttac
     4921 aatccagcgg tcttgagcca gcagcgatta acgtttcgct aaacctgccg ccggatctct
     4981 acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc atgccgccgg
     5041 tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa tccttcaacc
     5101 tgagcagtaa acaggaggcg aaccgcctgc tgctgcggat tccggtatta caaggtttgc
     5161 tggatggcaa aacggatgtc tctattccgg cgctgaaact gggcgcgacc aaccagctgc
     5221 gcttcgactt tgagtatatg aacccgatgc cgggcggttc ggtggataac tgtattacct
     5281 tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac ttctcgaagt
     5341 attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc ttcccgttca
     5401 gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaaacgcct aacgaagcac
     5461 agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc ttcccggcga
     5521 ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc gacatcatga
     5581 tcatcggtgg tatcccggac aaattaaaag acgataagca gatcgacctg ttggtgcagg
     5641 cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc attgtgccgg
     5701 acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt gcgatggcgg
     5761 cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg ctgctggcag
     5821 atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca
     5881 ccatgttcgg ttcggtcgcg gtgctccgcg agtccggtat caacagccta cgtgttggcg
     5941 acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc
     6001 atccgattct gctggcggtg ctggcggcaa tcagtgtgat attgctggca tgggtactgt
     6061 ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag taattgaaga
     6121 tgaatgtgtt gcgtagtgga atcgtgacga tgctgctgtt ggctgccttt agtgttcagg
     6181 cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt caggaagggc
     6241 gcgtcatcgc ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa agttacggta
     6301 tgttctttgc cctggcggct aacgaccgtg tagctttcga taatattctc gactggacgc
     6361 agaacaatct cgctcagggc tctttaaaag aacgtttgcc cgcctggctg tggggcaaga
     6421 aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt gatgtctgga
     6481 tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat accgacatcg
     6541 gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct gggctgggtt
     6601 ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt tttaacccca
     6661 gctacctgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc
     6721 tgcgcgaaac caatcaacgt ttattgctgg aaaccgcccc gaaaggtttt tcgccagact
     6781 gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca
     6841 gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc gatccgcaaa
     6901 aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa aacggttatc
     6961 cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg atcggttttt
     7021 ctgccgccat gctgcccttt ttacaaaacc gcgatgcgca ggccgttcag cgccagcgcg
     7081 tggccgataa ctttcccggc agcgatgcct attacaacta tgtgctgacc ctgtttggac
     7141 aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta cctgactggg
     7201 gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc tggccgtcat
     7261 gccgatggtc gaggcagcac caaccgctca gcaacagttg ctggagcaag ttcggttagg
     7321 cgaagcgacc catcgtgaag atctggtgca acagtcgtta tatcggctgg aacttattga
     7381 tccgaataac ccggacgtcg ttgccgcccg tttccgttct ttgttacgtc agggcgatat
     7441 tgatggcgcg caaaaacagc tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta
     7501 taaatcgtcg cggactacga tgctactttc cacgccggat ggtcgtcagg cactgcaaca
     7561 ggcacgattg caggcgacga ccggtcatgc agaagaagct gtggcgagtt acaacaaact
     7621 attcaacggt gcgccgccgg aaggtgacat tgctgtcgag tactggagta cggtggcgaa
     7681 aattccggct cgccgtggcg aagcgattaa tcagttaaaa cgcatcaatg cggatgcacc
     7741 gggcaatacg ggcctgcaaa acaatctggc gctattgctg tttagtagcg atcgccgtga
     7801 cgaaggtttt gccgtcctgg aacagatggc aaaatcgaac gccgggcgcg aaggggcctc
     7861 taaaatctgg tacgggcaga ttaaagacat gcccgtcagt gatgccagtg tgtcggcgct
     7921 gaaaaaatat ctctcgatct ttagtgatgg cgatagcgtg gcggctgcgc aatcgcaact
     7981 ggcagaacag caaaaacagc tggccgatcc tgctttccgc gctcgtgcgc aaggtttagc
     8041 ggcggtggac tctggtatgg cgggtaaagc cattcccgaa ctacaacagg cggtgcgggc
     8101 gaacccgaaa gacagtgaag ctctgggggc gctgggccag gcgtattctc agaaaggcga
     8161 tcgcgccaat gcagtggcga atctggaaaa agccctcgca ctggacccgc acagcagcaa
     8221 caacgacaaa tggaacagtc tgctgaaagt aaaccgctac tggctggcga tccagcaggg
     8281 cgatgctgcg ctgaaagcca ataatcctga ccgggcagaa cgcctgttcc agcaggcgcg
     8341 taatgtcgat aacaccgaca gttatgcagt gctggggctg ggcgatgtgg cgatggcgcg
     8401 aaaagattat cccgccgccg aacgttatta tcagcagacc ttgcgtatgg acagcggcaa
     8461 cactaacgcc gtgcgcgggc tggcaaatat ttaccgccag caatcgccag aaaaagctga
     8521 agcgtttatc gcctcgctct ctgccagtca gcggcgtagc attgatgata tcgaacgcag
     8581 cctgcaaaac gaccgtctgg cacagcaggc agaggcactg gaaaaccagg gcaaatgggc
     8641 gcaggcggca gcacttcagc ggcaacgact ggcgctggac cccggcagcg tatggattac
     8701 ttaccgactt tcgcaggatc tctggcaggc cggacaacgc agccaggccg atacgttaat
     8761 gcgcaatctg gcgcagcaga agtcgaacga cccagagcag gtttacgctt acgggctgta
     8821 cctacgctta cgggctgtac ctctctggtc atgaccagga cagagcggcg ctggcgcata
     8881 tcaacagcct gccgcgtgcg cagtggaaca gcaatattca ggagctggtt aatcgactgc
     8941 aaagcgatca ggtgctggaa accgctaacc gcctgcgaga aagcggcaaa gaggcagaag
     9001 cggaagcgat gctgcgccag caaccacctt ccacgcgtat tgacctcacg ctggctgact
     9061 gggcgcaaca acgacgtgat tacaccgccg cccgcgctgc atatcagaat gtcctgacgc
     9121 gggagccagc taacgccgac gccattcttg gtctgacgga agtggatatt gctgccggtg
     9181 acaaagcggc ggcacgtagc cagctggcga aactgcccgc taccgataac gcctcgctga
     9241 acacacagcg gcgcgtggcg ctggcacagg cgcagcttgg cgataccgca gcagcgcagc
     9301 ggacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga
     9361 tggtgctgcg tgatggtgcg aagtttgaag cgcaggcggg cgatccaacg caggcgctgg
     9421 aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg caggataacg
     9481 acacctttac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa cgtggcgtgc
     9541 gcagcgatgc ggcggacctc tatcgccagc aggatcttaa cgtcaccctt gagcacgatt
     9601 actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact accatgttgc
     9661 aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc gtcaatatga
     9721 acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg ggcacctgta
     9781 cattacagga ctgtagcggc aaccgcagcc agtcggattc cggtgccagc gtggcggtcg
     9841 gctggcgaaa tgacgtctgg agctgggata tcggtaccac gccgatgggc ttcaacgtgg
     9901 tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt tacaccgtta
     9961 acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa aaagactccc
    10021 cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg ctaagtctga
    10081 gctacgataa aggtgaagca aacggcgtct gggcatcgct tagtggcgac cagttaaccg
    10141 gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac tataaggtca
    10201 ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg cattacgaca
    10261 aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag gaatacctgt
    10321 cgtttgccat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg
    10381 cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc
    10441 tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    10501 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    10561 ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat ttcctgctct
    10621 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    10681 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    10741 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    10801 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    10861 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    10921 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    10981 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11041 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgcccggtgc
    11101 cgataatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtttactcgc
    11161 tcgaacgtcc ggcaaacccg cagccaattg cctatctggt actacaggcg gattccttcc
    11221 gtatgtataa gttcgtgatg agcaccctct caacgttagt gaccatttac ttacttttgt
    11281 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    11341 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    11401 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    11461 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    11521 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    11581 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    11641 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    11701 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    11761 gtgtacagga accgtggcac gcaatcacct taggtcagca agtgctcact atcatgagcg
    11821 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    11881 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    11941 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagccgccc
    12001 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcta
    12061 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12121 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12181 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12241 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    12301 aactgatgca cccaaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    12361 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    12421 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    12481 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    12541 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    12601 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    12661 cacaacgcga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    12721 acccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    12781 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    12841 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    12901 cgcaggtacc ccataacctt acaagacctg tggttttact aaaggacacc ctatgaaaac
    12961 ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg gtattctcct
    13021 tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg gattcgttaa
    13081 gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg gcattgcggg
    13141 catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt actttgaaat
    13201 tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc
    13261 cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt acgccgatca
    13321 ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggcga gcgtcattgg
    13381 cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt ttggttttgc
    13441 gctccaccgt ctgggcagca aaggccaact gatttttaac gtcatcgaaa gtttctcgca
    13501 ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg cgttcggggc
    13561 aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg ggcagctgat
    13621 tatctgtttc tatattacct gtatcctgtt tgtggtgcta gtattgggtt caatcgctaa
    13681 agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac tgctgattgt
    13741 actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga tggagaaact
    13801 cggctgccgc aaatcggtgg tggggctggt catcccgaca ggctactcgt ttaaccttga
    13861 tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca ctaacagtca
    13921 gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc
    13981 ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg cggtgggcca
    14041 tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt cagaagctcg
    14101 tgcgctgact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa
    14161 agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg atggcaaaac
    14221 gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc gagtgcgggt
    14281 gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc aggtttattt
    14341 cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt ttaacgtcat
    14401 ttagactgac tgacaacggg cagtttttta ttaccaggat agttgatcag gggttcacat
    14461 gcagggcaca aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta
    14521 tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg
    14581 tttacagtgg caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct
    14641 gctggttaat atcggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat
    14701 ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg
    14761 gcagcagggg atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac
    14821 gctgtttaat ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta
    14881 tctggcaaat gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca
    14941 aagtcaggac atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg
    15001 tctgaaaggg tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga
    15061 agcggaaaaa attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact
    15121 ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga
    15181 actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga
    15241 agcggtgagt attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac
    15301 gccgtggcag ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc
    15361 ccgcgaggcg ctgttctggc atgttcagca agcgttaagc gccagtaaca gcaaagacat
    15421 cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga
    15481 atcaccaaac gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt
    15541 tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga
    15601 gctgcagaaa ctgtttgccg cctatgcgcg ggctgatacc gatattctga tgggtcagcg
    15661 gatgcgttcg ctgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg
    15721 gcaggatttc cttaacagcc tgacggtgga gatgttaaat caggatctgc gtcagcagtt
    15781 gtcgaatgat atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa
    15841 agcgttgcag gcggcctggg atcaaatcat ggccccatcg actgctgctg ccgccacctc
    15901 tgtcgccacg gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct
    15961 gatggtgatg agttagcaca accatcaggt atttatggat gagctggata gaggggtaac
    16021 actttatcca gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat
    16081 cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc
    16141 cgcactgccg cccgtcaaac ataccgccag ataaccggcg ctgaaagagt cgccagctgc
    16201 ggtggtatcg atcacttttt ctttcggcag tttcaccgcc ggaacatcca ctaacccttc
    16261 gccagcaatg gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc
    16321 cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc
    16381 atcgtccagc gtcaggaagg cgatatccgt gcattccagc atttgttggt acacctgctg
    16441 tgtctcttct ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc
    16501 gccgttggcg cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa
    16561 gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat
    16621 cgccgcagac tgctcactct ccagccagaa tttggcggcg gcttcgttcc gccagtagta
    16681 gaacgtacgc tcgccggtgc tgtcggtttc aatgtagtaa aggcccggca gacggttttc
    16741 catccgttgg gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatttgctg
    16801 gctaaaactg tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac
    16861 ctgacgggcg atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc
    16921 cgcgcctttc tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat
    16981 agtcgtgaac ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc
    17041 aaccatatta aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc
    17101 cgttgtggag tgaggaaatt taccccggcc aggcggtaaa gttctgccct tgcgcgccga
    17161 taatctttgt cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg
    17221 caggttatcc agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt
    17281 ttttggttgc agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcggacgg
    17341 ttaatggccg tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg
    17401 ccgccggatc gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag
    17461 cagattgatt tgctggcgca aaaagccgac ttctttgtag agcacgacct gctggcatcg
    17521 gtcaatattg atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccaa
    17581 attgagcgtc ttccctggct gcgtttcgaa ctggtggaac acatccgcct gccgaaagat
    17641 tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggcaccggg
    17701 atggcaaatt tctctgcgct aagtgaagtg cgttatgact acatcaaaat cgcgcgagag
    17761 ctgttcgtga tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat
    17821 ctaatgaatc gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg
    17881 cgtgatgttc agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca
    17941 ccgatggaaa cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgctggg
    18001 ctggactatc tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca
    18061 ggcaaaataa ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt
    18121 ttgattgcaa cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg
    18181 gagttgaatc gtccgttcgc tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa
    18241 caagaaactg gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt
    18301 cttggtaatc caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca
    18361 acgctggcac cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa
    18421 aagcccgacg cgcgcctgat tcgcctctcc gaaaagaaca ataactggac gtttaatctt
    18481 gccaacgatg acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat
    18541 aatattcttt tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg
    18601 gaaatttttg ttgatccgtt aggcaagccg ctgccgttca gcgaagttac tggatcgaaa
    18661 ggtaaagcgg ataaagaaaa ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt
    18721 tataacggtg aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc
    18781 gaagggacgc cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt
    18841 gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct
    18901 ggcgattcac tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg
    18961 tttgaaacgg atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat
    19021 tatcgcggtt ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc
    19081 accggaaagc cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg
    19141 gacctgggac cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct
    19201 gaacagaaga agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc
    19261 ttcgaaaccg ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt
    19321 gagcatggca gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct
    19381 gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt
    19441 catctggaag gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg
    19501 aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac
    19561 ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac
    19621 ggcaacctga aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt
    19681 gggctgaatg tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg
    19741 aactgcgcgg cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct
    19801 ttcgatactg agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcagaacag
    19861 ctggatttga ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg
    19921 ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt
    19981 gcccgtggtg ctgttgctgc ggcactggca acgctggtaa caccgacggc ggcgttactg
    20041 gcactgatct caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg
    20101 aagaagtgat gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt
    20161 ggcaaagtgt gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc
    20221 cttgtgaatt caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt
    20281 ttcacactta ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg
    20341 tcaagccagc catcgccgcc agatataacc ccaccgcacc taacccgtag ttagtctgca
    20401 accaggctgc gatatacggt gcaatggaag ccccgagaat cgacgctacg ttgtaagaga
    20461 atgatgctcc ggtgtaacgc acttctgttg ggaacagctc tggtaacagc gcgcccatcg
    20521 gcccgaaggt cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg
    20581 ggttgccaga accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg
    20641 tgataattac catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca
    20701 ccatcacgcc aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca
    20761 ggccaagccc aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt
    20821 aaaacagcgt ataggttgcc agcatgatga acgtacccag tacggttacg cgaacatgtt
    20881 tggtcagcag cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa
    20941 acaccggcga ctcatgcagc gacacgcgaa catacaggcc gataatgacc agtaccgccg
    21001 agaagataaa tggcacacgc cagccccagc tcataaactg ctcgtcggtc agtagccagg
    21061 aaagcagcaa gaaagtgccg ttggcaaaga agaagccaat cggtgcgccc agctgtggga
    21121 aggagccata cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc
    21181 cgccccattc accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagcg
    21241 gggcgaaaat accaatcgtg gcatagcccg gcagcagacc aatcaccacg gttgaaatcc
    21301 ccatcgttag caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac
    21361 caaaaacggc agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact
    21421 gtagcgttgc tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg
    21481 ccgcagtggc gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga
    21541 caaggacttt attacgcgag ttgatcggcg tgtattcttg ctcgtggtcg agtgttgtgg
    21601 ctgttgcttg cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc
    21661 aaaagtgata tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg
    21721 gataatgttt aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag
    21781 tggataaaac tagtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta
    21841 tgagttggaa tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt
    21901 ttcaagaggt tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta
    21961 cggggagcca ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct
    22021 attttaaggc tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat
    22081 ccacgccgcg caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag
    22141 cacggaacca aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat
    22201 cacgatttta atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt
    22261 tttgcgcgga cggtggcgcg gtaaccgcca gaagatccag aagaaaagca gatagttggc
    22321 gaagatggaa atcgccaggc caatcaaccg ccacgtcggt ttcagccact caatgctgtt
    22381 caggtgcagg gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag
    22441 cgtcacaatc agcgcaatca acaaaccaat cagcgaaata aaatcacgta gatattttac
    22501 ccagaacttc tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcgcgaat
    22561 cgcttcacgc aggttaccca tccagttgat gccggaataa agcgccacgg ccaggccgac
    22621 aagccctaca gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc
    22681 tagcgtcgga tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg
    22741 atgggaggcc agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga
    22801 caaaaatgag aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg
    22861 ttctgtcgcg cgaatcaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt
    22921 ggtgacggtt tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt
    22981 aatcggctcg tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg
    23041 ctcttttgtc cttctgttgt ggtcattctt aaaaagtata gtcggtcagt ccaggtactc
    23101 tttcattacg ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg
    23161 ctgcggataa accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat
    23221 aagctgcccg gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag
    23281 accggcaaga ccggcggcga gatagttttc gctactgttt actgtcaaca tgccgccaga
    23341 cttaaaccac tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag
    23401 gtgcggtgta taacgcacca ttgcgtgtga agtcagatca tcggggcttt gcggataacc
    23461 aaagcgcgtc agatagtgcg gactggcaca gttgaccatg gtcagtttgc cgaggggacg
    23521 ggcgataacg ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg
    23581 aagaatatct accggacggt tatggctact cagttccagc tcaattcccg gatgctgata
    23641 gagaaattcc gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat
    23701 gcgtaattta ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag
    23761 attactcaac acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac
    23821 ccgtcgcgta gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg
    23881 gcgcgaaaca cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag
    23941 ctccgcgact ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg
    24001 ttgttcacag cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta
    24061 atgccgggtt tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg
    24121 gaatagcttt atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct
    24181 ttaaagtgaa tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt
    24241 tattttggct tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat
    24301 aaggaaatcc tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc
    24361 tgtaactatt tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc
    24421 aaatggcgaa ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta
    24481 acgtattcag gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt
    24541 attcatggaa tgaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggcc
    24601 agtcaggcag acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc
    24661 gatggcgatc aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt
    24721 cctgaggtta aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt
    24781 attcatttta atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg
    24841 gcggtaaata gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg
    24901 gaaaaggaca ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg
    24961 ttagcggcgg gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg
    25021 gtaaaagcgc atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg
    25081 gcaatgatgt taaatatctc ctcctg
//
LOCUS       DAZRUF010000038        24990 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZRUF010000038 DAZRUF010000000
VERSION     DAZRUF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.038"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            4..960
                     /gene="ytfQ"
                     /locus_tag="ACHXOL_001246"
     CDS             4..960
                     /gene="ytfQ"
                     /locus_tag="ACHXOL_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR6808271.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            1100..2602
                     /gene="ytfR"
                     /locus_tag="ACHXOL_001247"
     CDS             1100..2602
                     /gene="ytfR"
                     /locus_tag="ACHXOL_001247"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR6808272.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            2616..3638
                     /gene="ytfT"
                     /locus_tag="ACHXOL_001248"
     CDS             2616..3638
                     /gene="ytfT"
                     /locus_tag="ACHXOL_001248"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR6808273.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            3625..4620
                     /gene="yjfF"
                     /locus_tag="ACHXOL_001249"
     CDS             3625..4620
                     /gene="yjfF"
                     /locus_tag="ACHXOL_001249"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR6808274.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(4653..5651)
                     /gene="fbp"
                     /locus_tag="ACHXOL_001250"
     CDS             complement(4653..5651)
                     /gene="fbp"
                     /locus_tag="ACHXOL_001250"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR6808275.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            5827..7200
                     /gene="mpl"
                     /locus_tag="ACHXOL_001251"
     CDS             5827..7200
                     /gene="mpl"
                     /locus_tag="ACHXOL_001251"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR6808276.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(7283..7834)
                     /gene="yjgA"
                     /locus_tag="ACHXOL_001252"
     CDS             complement(7283..7834)
                     /gene="yjgA"
                     /locus_tag="ACHXOL_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR6808277.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            7928..9280
                     /gene="pmbA"
                     /locus_tag="ACHXOL_001253"
     CDS             7928..9280
                     /gene="pmbA"
                     /locus_tag="ACHXOL_001253"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR6808278.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            9464..9850
                     /gene="cybC"
                     /locus_tag="ACHXOL_001254"
     CDS             9464..9850
                     /gene="cybC"
                     /locus_tag="ACHXOL_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR6808279.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(9895..10359)
                     /gene="nrdG"
                     /locus_tag="ACHXOL_001255"
     CDS             complement(9895..10359)
                     /gene="nrdG"
                     /locus_tag="ACHXOL_001255"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR6808280.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(10517..12655)
                     /gene="nrdD"
                     /locus_tag="ACHXOL_001256"
     CDS             complement(10517..12655)
                     /gene="nrdD"
                     /locus_tag="ACHXOL_001256"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR6808281.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALITRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(13049..14704)
                     /gene="treC"
                     /locus_tag="ACHXOL_001257"
     CDS             complement(13049..14704)
                     /gene="treC"
                     /locus_tag="ACHXOL_001257"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR6808282.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(14754..16175)
                     /gene="treB"
                     /locus_tag="ACHXOL_001258"
     CDS             complement(14754..16175)
                     /gene="treB"
                     /locus_tag="ACHXOL_001258"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR6808283.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(16294..17241)
                     /gene="treR"
                     /locus_tag="ACHXOL_001259"
     CDS             complement(16294..17241)
                     /gene="treR"
                     /locus_tag="ACHXOL_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR6808284.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            17426..17479
                     /gene="mgtL"
                     /locus_tag="ACHXOL_001260"
     CDS             17426..17479
                     /gene="mgtL"
                     /locus_tag="ACHXOL_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR6808285.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            17620..20316
                     /gene="mgtA"
                     /locus_tag="ACHXOL_001261"
     CDS             17620..20316
                     /gene="mgtA"
                     /locus_tag="ACHXOL_001261"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR6808286.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(20522..20908)
                     /gene="ridA"
                     /locus_tag="ACHXOL_001262"
     CDS             complement(20522..20908)
                     /gene="ridA"
                     /locus_tag="ACHXOL_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR6808287.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(20981..21442)
                     /gene="pyrI"
                     /locus_tag="ACHXOL_001263"
     CDS             complement(20981..21442)
                     /gene="pyrI"
                     /locus_tag="ACHXOL_001263"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR6808288.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(21455..22390)
                     /gene="pyrB"
                     /locus_tag="ACHXOL_001264"
     CDS             complement(21455..22390)
                     /gene="pyrB"
                     /locus_tag="ACHXOL_001264"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR6808289.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(22394..22528)
                     /gene="pyrL"
                     /locus_tag="ACHXOL_001265"
     CDS             complement(22394..22528)
                     /gene="pyrL"
                     /locus_tag="ACHXOL_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR6808290.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(22809..23204)
                     /locus_tag="ACHXOL_001266"
     CDS             complement(22809..23204)
                     /locus_tag="ACHXOL_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR6808291.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(23335..24048)
                     /gene="bdcA"
                     /locus_tag="ACHXOL_001267"
     CDS             complement(23335..24048)
                     /gene="bdcA"
                     /locus_tag="ACHXOL_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR6808292.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            24119..24712
                     /locus_tag="ACHXOL_001268"
     CDS             24119..24712
                     /locus_tag="ACHXOL_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR6808293.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            24857..>24990
                     /gene="tabA"
                     /locus_tag="ACHXOL_001269"
     CDS             24857..>24990
                     /gene="tabA"
                     /locus_tag="ACHXOL_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR6808294.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     "
BASE COUNT         5848 a         6351 c         6721 g         6070 t
ORIGIN      
        1 cctatgtgga aacgcttact tgtagtctct gcagtctcgg cagccatgtc gtctatggcg
       61 ttggcagctc cattaaccgg aggattttcg caggtcggat cggaatccgg ctggcgcgcc
      121 gcagaaacca atgtggcgaa aagtgaggcc gaaaaacgcg gaattacgtt gaaaattgcc
      181 gatggtcagc aaaagcagga aaaccagatt aaagcggtac gttccttcgt tgcacaaggg
      241 gtggatgcga tctttatcgc tccagtggtt gcgacgggtt gggaaccggt attaaaagag
      301 gcgaaagatg ccgaaatccc ggtcttcttg ctcgaccgtt ccatcgatgt gaaagacaaa
      361 tctctctata tgaccaccgt tactgccgac aacatcctcg aaggtaagtt gatcggtgac
      421 tggctggtaa aagaagtgaa tggcaaacca tgcaacgtgg tggagctgca gggcaccgtt
      481 ggggccagcg tcgccattga ccgtaagaaa ggctttgccg aagccattaa gaatgcgcca
      541 aatatcaaaa ttatccgctc gcagtcaggt gacttcaccc gcagtaaagg caaagaagtc
      601 atggagagct ttatcaaagc ggaaaacaac ggcaaaaaca tctgcatggt ttacgcccat
      661 aacgacgaca tggtgattgg tgcaattcag gcaattaaag aagcgggcct gaaaccgggc
      721 aaagatatcc tcacgggttc cattgacggt gtaccggaca tctacaaagc gatgatggat
      781 ggcgaagcga acgccagtgt tgaactgacg ccgaacatgg cgggacccgc tttcgatgcg
      841 ctggagaaat acaaaaaaga cggcaccatg cctgaaaagc tgacgttaac caaatccacc
      901 ctttacctgc ctgataccgc aaaagaagag ttagagaaga agaaaaatat ggggtattga
      961 gggttgctat gcctgatgcc gattcgtagg ccggataagg cgctcgcgcc gcatccggcg
     1021 atggtgcact gaagcctgat gcgacgctta ccgcgtctta tcatgcctac tgggagcacg
     1081 ctttacaccg ggggaaacca tgacgaccga ccaacaccag gagatcctcc gcaccgaagg
     1141 attaagtaaa tttttccccg gcgtcaaagc gttagacaac gttgatttca gcctgcgccg
     1201 tggcgaaatc atggcgctgc ttggtgaaaa cggggcggga aaatcaacgc taatcaaagc
     1261 attaactggt gtataccacg ccgatcgcgg caccatctgg ctggaaggcc aggctatctc
     1321 accgaaaaat accgcccatg cacaacaact cggtattggc accgtctatc aggaagtcaa
     1381 cctgctaccc aatatgtcgg ttgctgataa tctattcata ggtcgcgaac ccaaacgttt
     1441 cggccttcta cgccgtaaag agatggaaaa gcgcgccacc gaactgatgg catcttacgg
     1501 tttcttcctc gacgtgcgcg aaccgcttaa ccgcttttca gtcgcgatgc agcaaatcgt
     1561 cgccatttgc cgggctatcg atctctccgc caaagtgctg atcctcgatg aacccaccgc
     1621 cagcctcgac acccaggaag tggagctatt atttggcctg atgcgtcagt tgcgcgatcg
     1681 cggcgtcagc ctgatctttg tcactcactt tctcgatcag gtctatcagg tcagcgatcg
     1741 gatcactgtc ttacgcaacg gcagtttcgt aggctgtcgg gaaacgcgcg agctaccgca
     1801 gatcgaactg gtaaaaatga tgctggggcg cgagctggat acccacgtgc tacagcgtgc
     1861 cgggcgaaca ttgttgagcg acaaacccgt tgccgcgttc aaaaattacg gcaaaaaagg
     1921 aacgatagca ccgtttgatc tcgaagtgcg ccccggcgag atcgtcggtc tggctggctt
     1981 gctgggatcc ggacgtaccg aaaccgccga agtgatcttc ggtatcaaac ctgctgacag
     2041 cggcacggcg ttgatcaaag gcaaaccgca aaccctgcga tcgccacatc aggcttcagt
     2101 actgggcatc ggattctgcc cggaagacag gaaaaccgat ggcatcatcg ccgccgcctc
     2161 ggtgcgggaa aatatcatcc tcgctctcca ggcccagcgc ggctggctac gacccatttc
     2221 ccgcaaagag cagcaagaga ttgccgaacg ctttatccgc cagcttggca ttcgcacacc
     2281 ttcaactgaa caaccgattg aatttctctc cggtggcaat cagcaaaaag tgctgctttc
     2341 acgttggcta ctgacccgac cgcaatttct gatccttgat gagccaaccc gtggcattga
     2401 tgttggtgcc cacgccgaga tcatccgcct gattgaaacg ctgtgtgctg acggtctggc
     2461 gctgctggtg atctcctccg aactggaaga gctggtgggc tatgccgacc gggtgatcat
     2521 catgcgcgat cgcaaacagg tggcggagat cccgctggca cagctttccg ttccggcgat
     2581 catgaacgcc attgcggcgt aaggagaaca gtgtgatgcc tcaatctctc cctgacacta
     2641 cgccgccaaa aaggcgtttt cactggccga cggggatgcc gcagctggcg gcactgttgc
     2701 tggtgctgct ggtcgatagc ctggtagccc cgcatttctg gcaggtggtg cttcaggatg
     2761 ggcgtttgtt cggtagccct atcgacattc ttaaccgtgc agctcccgtt gcgttactgg
     2821 cgattggtat gacgctggtg atcgccaccg gtggtatcga tctctctgtg ggggcggtga
     2881 tggctatcgc cggagccaca acggctgcga tgacggtcgc gggattcagc ctgccgattg
     2941 ttttgttaag cgccctgggc accggcatcc tagcgggatt gtggaacggc atactggtag
     3001 cgatcctcaa aattcagccg tttgtcgcca ccctgatcct gatggtcgcc gggcgcggcg
     3061 tggcgcaact gatcaccgcc gggcagatcg tcacgtttaa ctcgccggat ctctcatggt
     3121 ttggcagcgg atcgctgttg ttcctgccaa cgccggtcat tatcgcggtg ctgacgcttc
     3181 tcctgttctg gctgttgacc cgcaaaacgg cgctggggat gtttatcgaa gccgttggta
     3241 tcaacattcg ggcggcaaaa aatgccggag tcaacacacg aatcatcgtc atgctcacct
     3301 acgtgttgag cgggctatgt gcggcgattg cgggcattat cgtggcggcg gatattcgcg
     3361 gtgccgatgc caacaacgcc ggattatggc tggagctgga tgccattctc gcggtggtga
     3421 ttggcggtgg atcgctgatg ggcggtcgct ttaacctgct gctttcggga gttggggcgc
     3481 tgattattca gggaatgaat accggaattt tgctttcggg ctttccgcca gagatgaacc
     3541 aggtggtgaa agcggtggtg gtgctttgtg tgctgattgt tcagtcgcaa cgctttatca
     3601 gtctgattaa aggagtgcgt agccgtgata aaacgtaact tgccgcttat gatcaccatc
     3661 ggcgtctttg tgctgggcta tctctactgc ctgacgcagt ttcctggttt tgcttccaca
     3721 agagtgatct gcaatatcct gaccgataac gcctttcttg ggatcattgc cgttggcatg
     3781 acctttgtga tcctctccgg tgggatcgat ctctccgtcg gttcggtgat cgcctttacc
     3841 ggcgtgtttc tggcaaaagt gattggcgat ttcggcctct cgccgctgct ggcgtttccg
     3901 ctggtgctgg tgatgggctg tgccttcggc gcatttatgg ggcttctgat cgacgccctg
     3961 aagatcccgg cgttcatcat tacgcttgcg gggatgttct ttttgcgcgg cgtcagctat
     4021 ctcgtttcgg aagagtcgat tccgataaac catcccattt atgacacgct ctcaagcctt
     4081 gcgtggaaaa tccctggcgg cggtcgctta agtgcgatgg gactgctgat gttggcggtg
     4141 gtggttatcg gcatattcct cgcgcatcgt acccgttttg gtaatcaggt atacgccatt
     4201 ggcggcaacg caacgtcggc gaatctgatg gggatttcca ctcgcagcac cactattcgc
     4261 atttatatgc tctccaccgg actggcaacg ctggcgggga ttgtcttctc gatttatacc
     4321 caggccggat atgcgctggc gggcgtaggt gtggagctgg atgctatcgc ctcagtggta
     4381 attggcggta cgcttttgag cggtggcgtt ggaacggtat tagggacgct ttttggcgtg
     4441 gcgattcagg gactgattca gacttacata aactttgatg gcacgctgag ttcctggtgg
     4501 acgaaaatcg ccatcggtat tttgttgttt atttttatag cattacagcg tggattaacg
     4561 gtgctgtggg agaatcgtca gagttcgcca gtgacaagag tcaacattgc gcagcaataa
     4621 aaacgcctct ccgtgtggag aggcgcagga gattacgcgt ccgggaactc acggataaag
     4681 cgttcgacat cttcaaccat atggtcgttg ccgacaaaga acgaacggcg ctggtgcagg
     4741 gtttccggga tgatatccag aatacgctct ttgccatcgc tcgctttacc gcccgcttgt
     4801 tccgccagga atgccatcgg gttgcactca taaagcaaac gcagtttgcc gtccgggtgg
     4861 ctggcggtgc ttgggtagag ataaataccg cctttcagca ggttacggtg gaaatccgca
     4921 accagtgaac cgatataacg cgaggtatac gggcgattgg tggatttatc ttcttcctgg
     4981 cagaatttaa tgtacttctt caccccgttc ggaaacttaa tgtagtttcc ttcgttgatg
     5041 gagtaggttt tgcctttctc cgggaagcgc atccgttcct ggcacaggca gaaaacgccc
     5101 agcgaaggat cgtaagtaaa ggcgtgaaca ccgcatccgg tggtgtaaac cagcatggta
     5161 gaggagccgt ataccacgta acctgccgca acctgtttgt taccaggctg gaggaaatct
     5221 tcttccgtta ccggcgtgcc aacaggcgta acgcggcggt agatggagaa aatggtaccg
     5281 acagagacgt taacatcgat gttggacgag ccatccaggg ggtccatcag caccacgtat
     5341 tttgcgtatt cacagccttc aaagacgaca atctcatctt cttcttcaga ggcaatcccc
     5401 gcaacgatat cgcgtgcttt cagtgcggct ttcagttttt cattagcgaa caagtcgagt
     5461 ttttgctgaa cctcgccctg cgcgttctca gcaccgctgg cacccaggat atcaaccagt
     5521 cctgctttgt tgatatcgcg atggataatc ttggcgccca gttttattgc cgacagcaaa
     5581 gcagtgagct caccggtagc atgagaaaac tcgtgctgct tttcgacaat aaattcacct
     5641 aacgttttca taaaactttc cctgcaatgt ttatggagta aagcgaccgc aacaatctta
     5701 acaaataatc tcaatgttgc gctcaggtga atcgcgccag caaattgcgg attatcctga
     5761 aatgcgtttc tcacttgccc gacatatgcg taaaatgagc ggcagattaa aaaaggatag
     5821 tgacgtatgc gcattcatat tttaggaatt tgtggcacat ttatgggcgg tctggcgatg
     5881 ctggcgcgcc agttaggcca tgaagtaacg ggttcggacg ccaatgtgta tccgccgatg
     5941 agcaccttac ttgagaagca aggcattgag ctgattcagg gttacgatgc cagccagctc
     6001 gagccgcagc cggatctggt gattattggc aacgccatga cccgtggaaa tccgtgtgtg
     6061 gaagcggtac tggaaaaaaa catcccttat atgtcaggtc cacagtggct gcacgatttt
     6121 gtgctgcgcg accgctgggt gctggccgtt gccggtacac acggcaaaac caccaccgcg
     6181 ggaatggcga cctggattct ggaacagtgt ggttacaaac cgggatttgt gatcggcggt
     6241 gtgccgggga actttgaggt ttcggcgcgt ctgggcgaaa gcgacttctt tgttatcgaa
     6301 gcggatgagt acgactgcgc cttcttcgac aaacgctcta aatttgtcca ttactgcccg
     6361 cgtacgctga tcctcaacaa ccttgagttc gatcacgccg atatctttga cgacctgaaa
     6421 gcgatccaga aacagttcca ccatctggtg cgtatcgttc cggggcaggg ccgtattatc
     6481 tggccggaaa atgacatcaa cctgaaacag accatggcga tgggctgctg gagcgagcag
     6541 gagctggtgg gtgagcaggg tcactggcag gcgaaaaagc tgaccaccga tgcttccgaa
     6601 tgggaagttt tgctggatgg cgaaaaagtg ggcgaagtga aatggtcgct ggtaggcgaa
     6661 cataatatgc acaatggcct gatggcgatt gcagcggctc gccatgttgg tgtagcgtcg
     6721 gcagatgccg ctaacgcgct gggttcgttt attaatgctc gtcgccgtct ggagttgcgt
     6781 ggtgaagcga atggcgtcac ggtatatgac gattttgccc atcacccgac ggcgattctg
     6841 gcaacgctgg cggcgctgcg tggcaaagtt ggtggtacgg cgcgcattat tgctgtgctg
     6901 gaaccgcgct cgaataccat gaaaatgggg atctgcaaag acgatctggc accttcatta
     6961 ggtcgtgccg atgaagtctt cctgctgcaa ccggcgcata ttccgtggca ggtggcagaa
     7021 gtggcagaag cctgcgttca gcctgcacac tggagtggcg atgtggatac gctggcagat
     7081 atggtggtga aaaccgctca gcctggcgac catattctgg tgatgagcaa cggcggtttt
     7141 ggtgggatcc atcagaaact gctggatggt ctggcgaaga aggcggaagc tgcgcagtaa
     7201 ttcggcttca gcctgatata gcattgccgg ataaggcgtt tacgccgcat ccggcaaacc
     7261 atactcacat caacaacgaa aattaccctt cattctccgc cagttcgcgc agatactgga
     7321 aaatctggcg tgcggatttc ggcggcttat tcccttcttt ctctttcttc gcgttgcgga
     7381 tcagtgtacg cagttgctga cgatcggcat cgggccacag attcaatact tcagcgatgg
     7441 cgtcatcacc ttgatcgatc agacgatcgc gcaggttttc cagtttatgg aacagcacca
     7501 cctgctggtt gtgacggttt ttcagcttat ccagcgcctg acgaataggc tctacgtcgc
     7561 gctggcgcag catcttaccg atgagctgca actggcggcg gcgaccttcc atcttaatac
     7621 gctgggcgag ctcaatagcc gcgcgcagat ccgcatctaa cgggatctta tccagcgcgt
     7681 ttttccccag atcaacaatt tccgcgccaa ggcgttttag ctcctcggca tcacgtttaa
     7741 tttcactttt actgacccag ataatttcat cgtcttcgtc ttcgatgtca tcaccgggaa
     7801 cgtcgtcgag ccagtcttcg ggctgcttag tcatctcagg ctccttaaaa aaagaggcta
     7861 atgttaccag ttaagatgcg cactgaaaaa cggttctctg ttagacttca gagaaactct
     7921 ctacattatg gcacttgcaa tgaaagtaat ctctcaagtt gaagcgcagc gcaagattct
     7981 ggaagaagca gtttccactg cgctggagtt ggcctcaggc aaatcggacg gtgcggaagt
     8041 tgccgtcagc aagaccaccg gcattagcgt aagcacgcgt tatggtgaag tggagaatgt
     8101 tgaattcaat agcgatggcg cgctggggat cactgtttat caccagaacc gcaaaggtag
     8161 cgcatcatcc accgatttaa gcccgcaggc cattgcccgt actgtacagg cggcgctgga
     8221 tattgcccgt tatacctcgc cagatccctg tgccggcctg gcagacaaag agctgctggc
     8281 ctttgacgca ccagatctcg acttgttcca ccctgcggaa gtttccccgg atgaagccat
     8341 tgaactggcg gcccgcgcag aacaggcagc attacaggcg gataaacgta tcaccaatac
     8401 cgaaggtggc agctttaaca gccactacgg tgtcaaagtt tttggcaaca gccacggcat
     8461 gttgcagggt tactgctcaa cgcgtcattc gctctccagc tgtgtaattg ccgaagaaaa
     8521 tggcgatatg gagcgtgatt acgcctacac cattgggcgc gcgatgagcg atctgcaaac
     8581 gccagagtgg gttggggccg actgtgctcg ccgtacttta tcgcgcctgt taccgcgtaa
     8641 actctccacc atgaaagcgc cagtcatttt tgccaatgaa gtggcaactg ggctttttgg
     8701 gcatctggtg ggggcgatag cgggtggatc ggtttatcgt aaatctacct tcctgctgga
     8761 ttcgctgggt aaacaaattc tgccggactg gctgaccatt gaagagcatc cgcatctgct
     8821 gaaagggctg gcgtcgacgc cattcgacag cgaaggtgtg cgcaccgagc gtcgcgatat
     8881 tattaaagat ggcatcctga ctcagtggct gctgaccagc tattcggcgc gaaaacttgg
     8941 gctgaaaagc accggacatg cgggcggtat tcacaactgg cggattgccg gacaagggct
     9001 aagcttcgaa caaatgctca aagagatggg caccgggctg gtggtgacgg agctgatggg
     9061 ccagggcgtg agtgccatta ccggtgatta ttcccgtggt gctgcgggct tctgggtaga
     9121 gaatggcgaa attcagtatc cggtgagcga aatcaccatc gcaggtaatt taaaagatat
     9181 gtggcgcaat attgtcaccg tcggtaacga tattgaaaca cgcagtaata tacagtgtgg
     9241 ttctgtgctg ttgccggaga tgaaaatcgc cggacagtaa aaaaactggc gcgacctgcc
     9301 gcgctataaa ctaaaaaatt acacaaaatc attcgcactg catcgagacg gaaagtgaat
     9361 gagagccggg gagcgtactc gtagtacgtg acccggtcga atgagcgtag ctgtcgatga
     9421 ggcagtgtga aggatgaagt gtaaataaaa aaggaagtga gcaatgcgta aaagcctgtt
     9481 agctattctt gcagtctcct cgttggtatt cagttcggcg tcgtttgccg ctgatcttga
     9541 agacaatatg gaaaccctca acgacaattt aaaagtggtc gaaaaagccg ataacgcgac
     9601 gcaagtcaaa gacgcgttaa cgaagatgcg cgccgcagcg ctggatgcgc aaaaagcaac
     9661 gccgccgaag ctcgaagata aatcaccgga cagcccggaa atgaaagatt tccgccacgg
     9721 tttcgacatt ctggtcggtc agattgacga cgcgctgaag ctggcaaatg aaggtaaagt
     9781 aaaagaagcg caggctgctg cagagcaact gaaaacgacc cgcaacgcct atcaccagaa
     9841 gtatcgttaa ttcctcattt ccctgttgcc tgcactcagg taacagggaa cttatcatcg
     9901 caaatgatgc accacctgat tgctgctgcc gcgccagatc agggaagggt cttttaaatc
     9961 ctgcacaaat ttgccgtcga ccagcacgtt aatcaaatca acaacctgca tttgcgcagc
    10021 gttgagttcg tcgagtttat agcctgtcca cacccagatg tctttacccg gacactcggc
    10081 gcggatgcgt tttaccagtt tcagaatatc cggcacgttt tgcggatgca gcggatcgcc
    10141 gccggagagg gaaatcccct ggcgtttgat acgagtgtca ttcagatctt taatgatctt
    10201 gtcttccatt gctttggtaa atggctgacc agaatttacc cgccatgtgc ttttgttata
    10261 gcaaccgggg cattcatgaa cacacccgga gacaaacagg gtgcagcgag tgccggggcc
    10321 gttgacgatg tcgacaggat agtactgatg ataattcatt atttcgcctg gcttatcagt
    10381 ccgcgtgtag gctgcatccg gtaatcaatg cctgatgcga cgctgtcgcg tcttatcagg
    10441 cctacagccg ttgccaaatg taggccggat aaggcgttta cgccgcatcc ggcatttgct
    10501 taacgcagag tgaagattaa cctatctgcc cattccccaa atgtttaacg cggcgcttaa
    10561 cttcttcctg cttaccggcg ttaaacggac gcgcatccgg gctacctaaa tatccgcaca
    10621 cgcggcgagt taccgacaca cgggaggcgt catggttacc acatttcggg caagtgaagc
    10681 ctttgctggt gcactcgaac tcaccggtaa agccacactc gtagcactca tcaatcggtg
    10741 tattggtgcc gtaatacggt acatgctgat agctgtaatc ccagacatct tccagcgctt
    10801 tcaggttgtg ctgaatgttt ggatactcgc cgtagcaaat gaaaccaccg ttcgccagcg
    10861 gcgggtaagg cgcttcaaag tcgatcttgt cgtacgggtt caccttcttc tccacatcaa
    10921 ggtggaaact gttggtgtag taacctttat cggtcacgcc cggcaccacg ccaaactcag
    10981 cagtatcgag acggcagaag cggtcgcaca ggttttcact cggcgtgctg tagagactga
    11041 aaccataacc cgtttcttct ttccactgat ccactgcctg acgcagacgt tcaacaatgg
    11101 cgataccttt cgcgcgaagc tgctcgttgt cgtaaacatg ctcgccgccg aacagcgcgt
    11161 taatggtttc gtggatgccg atgtaaccca gcgaaataga cgcacgaccg tttttgaaga
    11221 tttcagaaac atcatcgtca gcattcagac gtacgccaca agcaccttcc atatagagga
    11281 tcggggcgac gcgcgctttc acgccttcga gacgagcgat acgggttatc agtgccttac
    11341 gtgccagcac cagacgttca tccagcagct tccagaaggt ggcttcatcg ccttttgctt
    11401 ccagagcaat acgcggcagg ttcaggctga tcacgccgag gttgttacga ccatcgtgga
    11461 tctgctcgcc gttttcattt tcccacacgc cgaggaagct gcggcagccc atcggggttt
    11521 taaacgaacc ggtgactttc actacctgat cgtagttcag gatatccgga tacatgcgct
    11581 tgcttgcgca ctccagcgcc agctgtttga tgtcgtagtt cggatcgcct tttttatggt
    11641 tcaggccatc gcgaatcgca aacaccagtt tcgggaacac cgcagtttta cggtttttac
    11701 cgaggcctgc gatacggtta cgcaggatcg attcctgaat caggcgcgat tccctgctgg
    11761 tgcccagacc aaaaccaaag gttacaaacg gcgtctgacc gttggcggtg tgcagggtgt
    11821 ttacttcgta ctccagcgac tggaaggcat cgtagcactc tttgatggta cgagagttag
    11881 cgtagccttc ggcgtccggg atgttccact cttctgcggt cttgcgatgt ttgttgtagc
    11941 tggcggtaac aaacggcgcc agcacttcat cgatacggtt aatggtggtg ccgccataaa
    12001 tatggctggc gacctgagca ataatctgcg cagttaccgc ggttgccgta gagatcgact
    12061 tcggcggttc aatctcggcg ttccccattt taaagccctg ggtcagcatg cctttcaggt
    12121 cgatcaacat gcagttgaac atcgggaaga acggcgagta atcgagatcg tgatagtgaa
    12181 tatcgccacg ctcatgtgcc tgcaccacgt cacgcggcag caggtgctgg cgtgcatagt
    12241 gtttagccac gatcccggcc agcaggtcgc gctgggttgg aatcaccttg ctgtctttgt
    12301 tggcgttttc gttgagtaac gaggcgttgg tctgctcgac cagaccacgg atctcttggt
    12361 tcaggcgacc gcgtttttca cgttcaatgt cgcgatcgtg acggtactcg atgtaagcac
    12421 gagccagttg tttgtatgga cccgacatca gctggttttc aactgcggtc tggatctcgt
    12481 tgatatccac ctggttgcgg ccctgcatct gctcgctgac aaccgcggca acagtggcgc
    12541 aataatcggc atcatcgact tccgctgctt tagctgcacg cagaatcgct tctttgatgc
    12601 gctctgattt aaacggcact ttgcagccgt ctcgtttcat cacatgcggt gtcatgatcg
    12661 ctccatattt ttaagaacag gttatccaca gaaattggga aaggcgtttt ccggtttttt
    12721 cgtttcgttt tccggtgctt tccgcaatcg ccatccgctt atccacaacg ccgcaccttt
    12781 ttcgtgggga gctgtagtag caattatagt cgattaatac aacatattgg gttgggacgc
    12841 attttaaagt ctatatatag tgctttgcat caaggatgtt ttaacttttt ttgatgtagc
    12901 tcaaagtaaa aagcagagcg tacggatgac gggcgctaca gcgttatgta aattttttaa
    12961 tgaatttgct ggttgaaaaa tcaacaaaaa caacatgctg acagacaaaa ccccggaatg
    13021 accggcatta ccggggctta gggaagattt acttctgtaa ccaccagaca gcctcaaaag
    13081 gccgtaaatt catggcacag ggttgtggtg aggcttcttc gtagttatgc atcacaagct
    13141 gccagttgcc gcgcatttgc cctggctgcc agggttggat ctcacggcta aggttggcaa
    13201 tgaccagcaa ggtttgcccc ttccattcac ggcgatagca ccacaataca gggctgtttg
    13261 gcagcagatc ctggtaattg ccccatgtca ggatggcttc ttgcttacgc agtgcgatta
    13321 acttttggta ggtgtaaaac accgaggagt catcggccag cgcggcttct acgttgattt
    13381 gttgatagtt atcgcccagg ccaatccacg gttcgccagc cgtaaacccg gcattatcgc
    13441 cgttgctcca ttgcatgggc gtgcggctgt tgtcacggga tttactggcg aggattgcca
    13501 ataactcgtc ggcatcacga ccatcgttgc gcagctcggc aaacatattg aggctctcca
    13561 cgtcgcgata gtcagtaatg cgcgtgaaat gcgggttggt catgccaatc tcttcgccct
    13621 ggtagatata cggcgttccc tgcatgccat gcagcaccat cgccagcatt tttgccgcag
    13681 gcacgcggta ttcaccttca tcaccaaagc gagaaacaat gcgcggctga tcgtggttac
    13741 accagaacaa ggcattccat gccacgttgt gcattccttg ttgccagtgg cggaacaatg
    13801 ttttcaacgc cacaaagtca ggtttagcca gcgtccattt ttcaccaccg ggataatcga
    13861 ccttcaggtg atgaaaatta aaggtcatcg acaattcact gcctgtcagt gccgcgtatc
    13921 gctggcaatg ctcaaggctg gtggaggaca tttcacctac ggtcattaac ccgcgtggcg
    13981 taaacacatc gcggttcatc tcgtgcaaaa actcgtgtgc tcgtggcccg tcggtgtaga
    14041 agcgacgccc gtcgccatcc aggtcttcag ggaaacgcgg gtctttggag atcagattca
    14101 ccacatccag gcgcaacccg tcgaccccac gatcggccca gaattcacag acttttttca
    14161 gctctgcgcg taccgctgga ttctcccagt tgagatccgc ctgttctggt gcaaagagat
    14221 gcaaatagta ctgttcgctt tccgcatgcc agcgccacgc actaccgcca aattttgaac
    14281 gccagttgtt cggtggcgtt tctggttcgc catcgcgcca gatataaaac tggcggtaag
    14341 ggctttcttt gttcagcgcc tcgcgaaacc aggcatgttg ggtagaggta tggttaaaca
    14401 ccatatcgag aatgatacga atcccgcgcg attttgcctg cgtcaccagt tcgtcaaaat
    14461 cgtccagcgt gccgtaggtg ggatcaatcg ccgtatagtt cgctacatcg taaccgttat
    14521 cgacctgggg agagacataa aagggggtta gccagatggc atcaacgccc agtttatgca
    14581 gatagtccag gcgttggata acgccacgta aatcgccggt gccgctaccc gtggtgtcct
    14641 gaaaactctt tggataaatc tggtagataa cgccgttttg ccaccagtgg ggaagattag
    14701 tcattacgtt attcctgcaa atgcgagggg gcgcaattgc gccccgaaga aaattaaaca
    14761 atgtccagcg tgcccaagcg gtatttccgc tgatagataa acgaggtgag tacaatcggg
    14821 atgatgatgg cgatagccat tgccagcgca aatacctgcc agtagctcgg ttgaatcgag
    14881 agaattcccg gcaggccccc tacgccgata ccattcgcca taacgccgtt caggccgcat
    14941 agcaatcctg ccagaccaga accaatcatc gcgcacagca tcgggaagcg atatttcagg
    15001 ttgatgccgt acattgcagg ctcagtgacc ccaagccagg cggagatagc ggcaggcacg
    15061 gagatctcgc gttcattgtg cttgcggctg gaaatgataa tgcctatcac ggcggagccc
    15121 tgagcgatat tcgacagcgc aatcagcggc cacactggcg tgccacccat gctttgaatc
    15181 atctgcaaat caatagcaag ggtggtctgg tgtacgccgg tgatcaccag cggcgcgtac
    15241 aggaagccaa acaatgccgc accaatcgga gcaaagctgc cggtcatcag gtgacgtacc
    15301 gcaaaggcaa cgccatcgcc aatcatgcga ccaaacggac caatcagcgc atgggcgagg
    15361 aacaccgcga ggatcagcga acagacgggc accaccacca gatagaggta atccggtacg
    15421 atgcgtttaa ggcgagtttc aataacgccc agcgccagcc cggctaacag tgccgggatc
    15481 acctgcgcct gatagcccac tttggcgatg ctgaacatgc caaagtccca cacttccggc
    15541 agctgctgcc cgagcagata agcgttcatc agctgggggg aaaccagtgt cacgccaagc
    15601 acgataccaa ggatcggcgt gccgcccatt tttttcaccg ctgaccagca aatcccgacc
    15661 ggcaggtaga agaaaatcgc ttcaccgatc aaccacagaa aatcgtagat cgtttgcagg
    15721 gaagggtaca tttgcgccag cgtctgaccg ttgctcatgg gcaaatcgcc gatcacattg
    15781 cgaaaaccga ggatcaaacc gccgctaatc aacgcgggca gcaacgggaa gaagatctcc
    15841 gcgaaatgag agatcaactg ctcatgccat ttcatattct gccgggcggc tttttttacc
    15901 tgctctttat caacctgcgc ctgtccggtt gacgctatta gtgcttgata gtaatcaccc
    15961 acgttggtgc caatcaccac ctgaaattgc ccggcattgg tgaaacagcc tttcaccatg
    16021 gggagttgct caatttcttt cggtctggca ttggccggtt ggttgaggac aaagcgcagg
    16081 cgagtaatac agtggctcac cgtcgcaata ttgccgcgcc cgccgaccag ttcaatcaac
    16141 cgatcgatat ccgtttggtt tattttgctc atcataaagc cccatggcag atgacatttt
    16201 tggttggctg cagaatatat tgcgcggaaa aatttaaaaa cgggaacgtt cccgaaatgc
    16261 agcgaagatc acaatttatc gttcaggaaa cgatcaggac agggtggcgg ggatgatgat
    16321 ttgttgcggt tcgctgcgcc cggttacctg tgcgatcaat tggcaggccg cctggcgtcc
    16381 agcttcggcg taaccgggat ctacggtgac gatctccgga tggaggaatt tcattaacgg
    16441 tgtattaccg acgctcgcca gttgcaaggt gtcgatgcgt tgctcttgca ggtatttact
    16501 tgcgccaagt gccagcgtgt cggttgcgca cagtaacgca gtagtttcag gcgtaatcac
    16561 ttttgccacg ttctcatagc cttgcttcat agcaagccct ggcagagcgg caacgggatg
    16621 cagtttatgc gctttgcaga acgccaggta ggcttcgtga cgtcgcttac cggttgtcac
    16681 gtcactatgc ggcacgccga gataactgat attacgatgc ccctggtcat acagccgttg
    16741 catcaggatc ttgattgccc cttcgtcgtc ataacagacc gaagcaaagc cttttgcgtc
    16801 acgcgccagc agaaccagcg atgactgcca gtgggctaac atttcttctg ttatgccagt
    16861 aaaaccgaac agcactacgc cgtcgatatt acgccgtttc agcaccccca aatgttcggc
    16921 aactaattgc ggggaaaact ggctttccat catgattggg tcgtaacctt gttcatagaa
    16981 cgctggcagc atggtttgaa cggcgagatt ttctgacaac gaatccagac gggtaacaat
    17041 gatggcgacc actttatcgc tttgcccacg catagcgcgc gcagagcggg aaggggaaaa
    17101 tccatgctga ttcatcactg cttcaacacg ctcgcgggtg cgctggctca cgccgctttc
    17161 gttattcagc acccgggaaa ctgtagattt ccccacgccg cttaagcgtg cgatgtcttt
    17221 gatagtcagc cgattttgca tcctgttgtc ctgtaacgtg ttgtttaatt atttgagcct
    17281 aactttaccc gtgcattcag caatgggtaa agtctggttt atcgttggtt tagttgccag
    17341 caagtattat atcgccataa atgctacgaa tattattgga ttctccttat tatttgcggc
    17401 gcttttttca cttaccggag gttatatgga acctgatccc acgcctctcc ctcgacggag
    17461 attaaaactt ttccggtaag cccgtctttt cacggcgcta ccggatgcgt aaggccgtga
    17521 cgttttaacg tccctgctca gctttattac cttcaggtaa ggcttcgcca cgcctgaaga
    17581 catttctgta ctgtttcaga cagtgcggag ggactcctta tgtttaaaga aatttttacc
    17641 cggctcattc gccatttacc ttcccgtctg gttcatcgcg atccattgcc tggcgcgcag
    17701 cagacagtga atacggcggt cccgccgtcc ttaagtgcgc attgcctgaa aatggcggtg
    17761 atgcccgaag aagaattgtg gaaaacgttc gacacccatc cggaagggtt aaatcaggcg
    17821 gaagtggaat ctgcccgcga acaacatggt gaaaataaat tacccgcaca acaaccgtcg
    17881 ccgtggtggg tacatttatg ggtctgctat cgcaacccat ttaatatttt actcaccatt
    17941 ctcggcgcta tttcttacgc cactgaagat ttatttgccg cgggcgttat cgcgctaatg
    18001 gtcgctattt ctacgttgct gaactttatt caggaagcac gttccactaa agcggcagat
    18061 gccctgaaag cgatggtcag caataccgcg acggtgctgc gcgtaattaa cgacaaaggc
    18121 gaaaatggct ggctggagat cccgatcgac cagctggtgc ccggcgatat tataaaactg
    18181 gcggcggggg atatgatccc ggcagattta cgtatcttgc aggcgcggga tctgttcgtc
    18241 gctcaggcgt cgttaaccgg tgagtctctg cccgtggaaa aagccgctac cactcgccag
    18301 ccggagcaca gcaatccgct ggagtgtgac accctgtgtt ttatgggcac caccgtggtg
    18361 agcggcacgg cacaagcaat ggtgattgct acaggagcca acacctggtt tggtcaactg
    18421 gcggggcgtg ttagtgagca ggaaagcgag ccgaatgcct ttcagcaggg gatcagccgc
    18481 gtcagtatgc tgctgattcg ctttatgctg gtgatggctc cggtggtgtt gcttatcaac
    18541 ggttacacca aaggcgactg gtgggaagcg gcactgtttg cgctttcggt cgcggtaggc
    18601 ttaacgccgg aaatgttgcc gatgattgtt acctcgacgc tggcgcgcgg cgcagtaaag
    18661 ctgtcgaaac agaaagtgat cgtcaaacat ctggatgcta ttcagaactt tggcgcaatg
    18721 gatattctgt gcactgataa aactggcacc ctgacacagg acaaaattgt gctggagaat
    18781 cataccgata tctccggtaa aaccagcgaa cgcgtgctgc atagcgcgtg gttgaacagt
    18841 cattatcaga ccggacttaa aaacctactc gatacagcgg tgctcgaagg tacggatgaa
    18901 gagtcagcgc gctctctggc cagccgttgg caaaaaattg atgagattcc gtttgatttc
    18961 gagcgtcgcc ggatgtcggt ggtagtggca gaaaataccg agcaccatca gctggtttgc
    19021 aaaggtgcat tgcaggaaat cctcaatgtg tgttcgcagg tgcgtcacaa tggcgagatt
    19081 gtgccgctcg atgacatcat gctgcgtaag attaagcggg ttactgatac gctgaatcgt
    19141 caggggctgc gcgtggttgc ggtggcgacg aaatacctgc cagcgcgtga aggagattac
    19201 cagcgggcgg atgaatccga cctgatcctc gaaggatata ttgctttcct tgatccgcca
    19261 aaagagacaa ctgctccggc actgaaggca ttaaaagcga gtggaattac cgtaaaaatc
    19321 cttactggcg acagtgagtt agttgcagcg aaagtgtgcc atgaagtggg gctggacgcg
    19381 ggagaggtgg tcattggtag tgatattgaa acgctatctg acgacgaact ggcaaatctc
    19441 gcacagcgca ccacgctgtt tgctcgtctg acgccgatgc ataaagaacg cattgtgacc
    19501 ttactgaagc gcgaagggca tgtggttggc tttatgggcg atggtattaa tgatgcgccc
    19561 gccttacgcg ctgcggatat cggcatttct gtggacggcg cggtagatat tgcccgtgaa
    19621 gcggctgata tcatcctgct ggaaaaaagc ctgatggtgc tggaagaggg ggttattgaa
    19681 ggacgtcgca ctttcgccaa catgctgaaa tacatcaaaa tgacggcgag ctccaacttc
    19741 ggtaatgtgt tcagcgtgct ggtggcgagt gctttcttgc ccttcctgcc gatgttgccg
    19801 ttacacttgc ttattcagaa cctgctgtat gatgtgtcac aggtggcgat cccgtttgat
    19861 aacgtcgacg acgagcaaat tcaaaagccg cagcgttgga atccggcgga tctggggcgc
    19921 tttatgatct tcttcggacc gatcagctcg atcttcgata ttctgacgtt ttgcctgatg
    19981 tggtgggtat tccatgccaa cacgccggaa acgcaaacgc tgttccagtc gggatggttt
    20041 gtggtgggct tgctgtcgca aacgctgatt gtgcatatga tccgcacccg ccgtgtgccg
    20101 tttattcaga gctgtgcatc gtggccgtta atgatcatga ccgtgatcgt gatgattgtc
    20161 gggatcgcat tgccgttttc accgctggcc agttatctgc aactgcaggc gctgccgtta
    20221 agctatttcc cgtggctggt tgcgattctg gcagggtata tgacgttaac ccaactggtg
    20281 aaaggatttt atagccgtcg ttacggctgg caataaagaa tcaactgggc atggtggcct
    20341 ggattcagat tgcagacaaa gtgagttttg ttcatgccgg atgcggcgta aatgccttat
    20401 ccggccaaca aattcaataa attgcaggaa tcacgtaggc ctgataagcg tagcgcatca
    20461 ggcgattttg tgtttgtcat cagacttaat ccgggcatga tagcccggat ttccatcaag
    20521 attagcgacg aacagcgatc gcttcgatct caattttcac gtctttcggc agacgggcaa
    20581 cttcaacgca agaacgtgcc gggaaggtgg cattgtgttc ggtgaagaag gcttcgtagg
    20641 tagcattaac ggttgcgaag tcgttcagat ctttcacaaa cacggtggtt ttaacgatgt
    20701 cgcccacttt caggccagcg gcttcgacga tcgcttttac gttatccagc gactgacgtg
    20761 cctgtgcagc gacgtctgcc ggtacttcgc ccgttttcgg atttaccggg atctgaccgg
    20821 aggtgatgat catattgccc agatcaacgc cctgtacgta aggaccgata gctgccggtg
    20881 cattttccgt cgcgatagtt ttgctcatga tttctccttt attacagcgg taaaaagtct
    20941 ggctcattat agggagccag acttttatta ccaaccgcaa ttaattggcc agcaccacat
    21001 tatgggaaaa ctctttttca cagtatttgc atttgagcgc gatgtcattg gcgcgttttc
    21061 gcacggcaaa gctggaagaa accggttcgg catggctgat acagttgctg ttcgggcaga
    21121 ccagcacatt gtcgatgcgc tccggcagac tggggcgtga tttacccacc acttcatagt
    21181 tgtcgatacg gttaaccgtg gcttgcggcg catacaatgc cagttgatct acctgatctt
    21241 cactcaaaaa ggtattttcg attttgatca gatctttgcg gcccatctcg ccagaaggca
    21301 ggttcagacc aatggtgatg cgctgatccg tttcggtcag cttgaacaga ctcaacagct
    21361 taaaaccgat ctgggcgggg atatggtcaa ttaccgtgcc gcgtttaata gcttcaacct
    21421 gcaatttatt atcgtgtgtc atctctattt ccccttacag taccagatcg cgattcagaa
    21481 ccagtgccag taacgcctgg cgagcgaaaa tcccgttgcc tgcctgctgg aagtaccagg
    21541 cgtgtggcgt tttatcaaca tccgtcgcaa tctcatcaac acgcggcagc ggatgcagca
    21601 ctttcatatt ggctttggcg ttgtggagat cgctggcgcg aagaacaaac tgcgctttca
    21661 cgttggcgta ctcggacggg tccagacgct ctttttgcac gcgggtcatg tacaggatgt
    21721 ctacttccgc catcacttct tcaatagagc tgtgcagact ccaggcgatc cctttttcat
    21781 caagcatatc cagaatgtat tgcggcattg ccagcgcgtc cggcgcgatg aagtaaaaac
    21841 ggttgccgtc gaacttcgct aacgcctgag tcagggagtg aacggtgcgg ccatatttca
    21901 ggtcaccaac cattgcgacg tggagattgt ccagacgccc ctgggtttcc tgaatagtga
    21961 ataagtccag caaggtttgc gtcggatgtt ggttggagcc atcaccggca ttcagtaccg
    22021 gtacattgcc ggaaaactcg gtggccaggc gcgccgcacc ttcctgcgga tgacgcatca
    22081 ctatcgcatc gacgtaagtg ctgataaccg aaatggtatc ggccagcgtt tcgccctttt
    22141 tacccagtga tgtattggcg ctgtcggaga agcccaccac gctggcaccc aggcggtgca
    22201 tggaagtttc gaaagagagg cgggtacggg tagaggcttc gaagaaacag ctggcaatga
    22261 ctttgtgctt caacagctct ggttgcgggt ttgctttcag tttcgccgct gtcgccagca
    22321 ccagattaag gtcatcgcga ctaaggtcgt ttatggaaat gatatgtttc tgatatagcg
    22381 gattagccat cttttatctc ctgacgcctg ggcaaaaaaa agcccctcga ttgaggggct
    22441 gggaatgggt gatcaacggg aagaaaaacg gcaggccagc gtcttttttc agacgcggta
    22501 agacaaaatg tcgaacacac tgaaccatac atcctcccgg caaattgtcc ggcattatac
    22561 tcatcgtcag aagcggtgca agcatttgat gcaatatttt gtcagcgcaa acggtttatt
    22621 tgaattataa gtcaaaatac atgcattttt atttgttgtg attctgcagg ggaacggtcg
    22681 ttcggtatca ataattgcag acatttcctg ctttttcctt tttcactttc acgcaatcag
    22741 atatgcattt tattccattc atccgactta tagggcggag tgtgaaaacg aacggctaac
    22801 actattgctt actgctcagg gatgcgcgct atcactttaa tttcaaaatc aaagcctgcc
    22861 agccatgtaa cacctaccgc cgtccagttt ggataaggtg gggcgctaaa tatttcattt
    22921 ttcaccgtca tgatgtcttc aaattggttt tctggatcgg tatggaagct cgtaacatca
    22981 atgatatcgt caaaagtgca tcccgcagca gccagggtcg catgcaaatt atcaaatgcc
    23041 agtctgactt gttgctgaaa atcgggttct ggtgttccgt cctctcgact tcctacttgc
    23101 ccggaaacaa acagcaaatc gccggaacga atagccgcag aataacgatg ttcagcatac
    23161 agtgaatgtc ggcctgcagg gaaaacagcg gttctttcta ccatttggtt atcctcaaga
    23221 tttacgacat gaacagaaga tttatattta ccaggagccg cttttagcgg acgacgtgag
    23281 taaacaaaac ccagacatca tgggaaatgg ctgggcttaa ttgagcgtag tcggttatgc
    23341 gccaaacgcg ccatcaatgg tatgcatcgc gccggtaaca aaactggctt ctggccctgc
    23401 taaccatgcg accataccag cgacctcttc cggttgccca tgtcttttga tagccataaa
    23461 actatgcaac atatcgcgca ttggcccgtt ggcgggatta gcgtcggtat caattggccc
    23521 tggctggacg acgttgatgg tgatcccacg cggtccgaaa tcacgggcca gctcgcgcgc
    23581 catgccttgc agggcagatt tactggcggc ataagcagcc atgcctgcaa caggcatacg
    23641 atcgccattc acggagccga tgattaagat gcgcccgcct tcgggcatct gccgggcggc
    23701 ttcaacagag gcatgataag gagcatgaat attgattttg aaaaggcgat caatatcgtc
    23761 ggcattcaat tccagggcat cgccaaagac gccaatacct gcatttacca ccaggatatc
    23821 caatgcgccg ctcttacgaa cgacatcaat gacagcgtct ctgtcagcac tatctgtgaa
    23881 tactgctgtc gctccagtct tttgtgccag gtgttcagcg gcatctttcg accccgcata
    23941 ggtgaatcgt acattggccc catcggtgac gaaacgacgt acgatagcgg caccgatacc
    24001 acgactgcca ccgaggatga gaactgtctt acctgtaaaa gcgcccataa ggactccttg
    24061 atttattatg taacatgcat tacaaaactg ttttaacttt ctgtcaacaa gttttgtaat
    24121 ggtcactaaa aaacaatctc gcgttccagg tcgtcccaga cgtttcgctc ctgagcaggc
    24181 agtctctgcg gcaaaagtgc tttttcacca aaaaggtttc gatgctgtca gtgttgctga
    24241 agttactgat tatcttggta ttaacccccc gagcctctac gcggcttttg gcagtaaggc
    24301 tgggttattt agccgtgtac tcaatgaata tgtcggtacg gaagctattc cgcttgccga
    24361 tattcttcgt gatgatcgtc cggtagggga gtgcctggct gaggtattaa aagaagcggc
    24421 gcgcagatat agccaaaacg gcggctgcgc tggctgtatg gttcttgaag gcattcatag
    24481 tcatgatcca caagcgcgtg atattgccgt tcaatattat cacgccgcag aaacggccat
    24541 ttatgactat atcgccaggc ggcatccaca aagcgcacaa tgtgtgactg attttatgag
    24601 taccgtgatg tcagggcttt ctgcgaaggc tcgggagggg cactccatcg aacaactctg
    24661 tgcaacagct gcactggcgg gggaagcgat aaaaactatt ctcaaggagt gatgcgggcc
    24721 ttgacccgaa aggcgggaac gcgcctgccg ataagttgtg ataagacaat aattcacgca
    24781 ttaaggctag cggaattgat catcttttcg tataacgatg gaaatgaaac attgttttaa
    24841 ttaaggagtg gaaaagatga tcatcggaaa tattcataat cttcagccgt ggctacccca
    24901 ggagttacgc ctggcgattg agcatatcaa agcacacgtt acggcagaaa cgccaaaggg
    24961 caagcacgat atcgaaggca atcgtctgtt
//
LOCUS       DAZRUF010000039        24041 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZRUF010000039 DAZRUF010000000
VERSION     DAZRUF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.039"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..127)
                     /locus_tag="ACHXOL_001270"
     CDS             complement(<1..127)
                     /locus_tag="ACHXOL_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR6808295.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATE"
     gene            complement(185..418)
                     /locus_tag="ACHXOL_001271"
     CDS             complement(185..418)
                     /locus_tag="ACHXOL_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR6808296.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            807..1352
                     /locus_tag="ACHXOL_001272"
     CDS             807..1352
                     /locus_tag="ACHXOL_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR6808297.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1327..3252
                     /locus_tag="ACHXOL_001273"
     CDS             1327..3252
                     /locus_tag="ACHXOL_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR6808298.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3249..3455
                     /locus_tag="ACHXOL_001274"
     CDS             3249..3455
                     /locus_tag="ACHXOL_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR6808299.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3452..5053
                     /locus_tag="ACHXOL_001275"
     CDS             3452..5053
                     /locus_tag="ACHXOL_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR6808300.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5034..6353
                     /locus_tag="ACHXOL_001276"
     CDS             5034..6353
                     /locus_tag="ACHXOL_001276"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR6808301.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIACLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6363..6695
                     /locus_tag="ACHXOL_001277"
     CDS             6363..6695
                     /locus_tag="ACHXOL_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR6808302.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            6751..7776
                     /locus_tag="ACHXOL_001278"
     CDS             6751..7776
                     /locus_tag="ACHXOL_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR6808303.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7818..8216
                     /locus_tag="ACHXOL_001279"
     CDS             7818..8216
                     /locus_tag="ACHXOL_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR6808304.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8228..8581
                     /locus_tag="ACHXOL_001280"
     CDS             8228..8581
                     /locus_tag="ACHXOL_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR6808305.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8593..9171
                     /locus_tag="ACHXOL_001281"
     CDS             8593..9171
                     /locus_tag="ACHXOL_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808306.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9168..9563
                     /locus_tag="ACHXOL_001282"
     CDS             9168..9563
                     /locus_tag="ACHXOL_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR6808307.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            9571..10311
                     /locus_tag="ACHXOL_001283"
     CDS             9571..10311
                     /locus_tag="ACHXOL_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808308.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10327..10749
                     /locus_tag="ACHXOL_001284"
     CDS             10327..10749
                     /locus_tag="ACHXOL_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR6808309.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            10731..11165
                     /locus_tag="ACHXOL_001285"
     CDS             10731..11165
                     /locus_tag="ACHXOL_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR6808310.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11158..13719
                     /locus_tag="ACHXOL_001286"
     CDS             11158..13719
                     /locus_tag="ACHXOL_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR6808311.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            13716..14045
                     /locus_tag="ACHXOL_001287"
     CDS             13716..14045
                     /locus_tag="ACHXOL_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808312.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14045..14743
                     /locus_tag="ACHXOL_001288"
     CDS             14045..14743
                     /locus_tag="ACHXOL_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR6808313.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14749..15492
                     /locus_tag="ACHXOL_001289"
     CDS             14749..15492
                     /locus_tag="ACHXOL_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR6808314.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15489..16061
                     /locus_tag="ACHXOL_001290"
     CDS             15489..16061
                     /locus_tag="ACHXOL_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR6808315.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNIYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16122..19619
                     /locus_tag="ACHXOL_001291"
     CDS             16122..19619
                     /locus_tag="ACHXOL_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR6808316.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            19690..20206
                     /locus_tag="ACHXOL_001292"
                     /pseudo
     CDS             19690..20206
                     /locus_tag="ACHXOL_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20271..23354
                     /locus_tag="ACHXOL_001293"
     CDS             20271..23354
                     /locus_tag="ACHXOL_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR6808317.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTLETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQH
                     SQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            23354..23870
                     /locus_tag="ACHXOL_001294"
                     /pseudo
     CDS             23354..23870
                     /locus_tag="ACHXOL_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         5686 a         6020 c         7348 g         4987 t
ORIGIN      
        1 tctctgtggc taaatagtaa atgtaatgtg agacattgtg acgttttagt tcagaataaa
       61 accagtgata gtttaaatta tttcgcactt tatcgaatat ttgtttaaaa atggcaacct
      121 gagccattgt agtaccttcc atgtgatatg agggggcgta gtctgcacga ttatctaaat
      181 tgcttcaatc tggtctaacc tgttttctga gcaattcagt aatgtcactc ttttctttgt
      241 ttgcttcagg cgaaactctt ttttctgagc acagtctccg gcggcaggct tcaatgaccc
      301 aggctgagaa attcccggac cctttttgaa caagagcgat gttaatttgt tcaatcattt
      361 ggttaggaaa gcggatgttg cgggttgttg ttctgcgggt tctgttcttc gttgacatga
      421 ggttgccctg tattcagtgt cgctgatttg tattgtctga agttgttttt acgttaagtt
      481 gatgcagatc aattaatacg atacctgcgt cataattgat tatttgacgt ggtttgatgg
      541 cgtagatgca cgttgtgaca tgtagatgat aattattatc attttgcggg tcctttccgg
      601 cgatccgaca ggttacgggg cggaaggcgc gcgggttttc gctatttatg acaattttcc
      661 ggtttaaggc gtttccgttc ttcttcgccg taacctaatg tttttattta aaacaccccc
      721 tgaaaagaaa ggaaacgaca ggtgctgaaa acgggctttt tggcctctgt cgtttccttt
      781 ctctgttttt gtccgtggaa tgaacaatgg aagtcaacaa aaagcagctg gctgacattt
      841 tcggtgcgag tatccgtacc attcagaact ggcaggaaca gggaatgccc gttctgcgag
      901 gcggtggcaa gggtaatgag gtgctttatg actctgccgc cgttataaga tggtatgccg
      961 aaagggatgc tgaaattgag aacgaaaagc tgcgccggga agttgaagaa ctgcggcagg
     1021 ccagcgagac agatctccag ccagggacta ttgagtacga acgccatcga cttacgcgtg
     1081 cgcaggccga cgcacaggag ctgaaaaatg ccagagactc cgctgaagtg gtggaaaccg
     1141 cattctgtac tttcgtgctg tcgcggatcg caggtgaaat tgccagtatt ctcgacggga
     1201 tccccctgtc ggtgcagcgg cgttttccgg aactggaaaa ccgacatgtt gatttcctga
     1261 aacgggatat catcaaagcc atgaacaaag cagccgcgct ggatgaactg ataccggggt
     1321 tgctgagtga atatatcgaa cagtcaggtt aacaggctgc ggcattttgt ccgcgccggg
     1381 cttcgctcac tgttcaggcc ggagccacag accgccgttg aatgggcgga tgctaattac
     1441 tatctcccga aagaatccgc ataccaggaa gggcgctggg aaacactgcc ctttcagcgg
     1501 gccatcatga atgcgatggg cagcgactac atccgtgagg tgaatgtggt gaagtctgcc
     1561 cgtgtcggtt attccaaaat gctgctgggt gtttatgcct actttataga gcataagcag
     1621 cgcaacacac ttatctggtt gccgacggat ggtgatgccg agaactttat gaaaacccac
     1681 gttgagccga ccatccgcga tattccgtcg ctgctggcgc tggctccgtg gtatggcaaa
     1741 aagcaccggg ataacacgct cactatgaag cgtttttcca atggtcgtgg cttctggtgc
     1801 ctgggcggta aagcggcaaa aaactaccgt gaaaagtcgg tggatgtggc gggttatgat
     1861 gaacttgctg cctttgatga ggatattgaa caggaaggct ctccgacgtt ccttggcgac
     1921 aaacgtattg aaggctcggt ctggccaaag tccatccgtg gctccacgcc caaagtgaga
     1981 ggcacctgcc agattgagcg tgcagccagt gaatccccgc attttatgcg ttttcatgtt
     2041 gcctgcccgc actgcgggga ggagcagtat cttaaatttg gcgacaaaga gacgccgttt
     2101 ggcctcaaat ggacgccgga tgacccctcc agcgtgtttt atctctgcga gcataatgcc
     2161 tgcgtcatcc gccagcagga gctggacttt actgatgccc gttatatctg cgaaaagacc
     2221 gggatctgga cccgtgatgg cattctctgg ttttcgtcat ccggtgaaga gattgagcca
     2281 cctgacagtg tgacctttca catctggaca gcgtacagcc cgttcaccac ctgggtgcag
     2341 attgtcaaag actggatgaa aacgaaaggg gatacgggaa aacgtaaaac cttcgtaaac
     2401 accacgctcg gtgagacgtg ggaggcgaaa attggcgaac gtccggatgc tgaagtgatg
     2461 gcagagcgga aagagcatta ttcagcgccc gttcctgacc gtgtggctta cctgaccgcc
     2521 ggtatcgact cccagctgga tcgctacgaa atgcgcgtat ggggatgggg gccgggtgag
     2581 gaaagctggc tgattgatcg gcagattatt atgggccgcc acgacgatga acagacgctg
     2641 ctgcgtgtgg atgaggccat caataaaacc tatacccgcc ggaatggtgc agaaatgtcg
     2701 atatcccgta tctgctggga tattggcggg attgacccga ccattgtgta tgaacgctcg
     2761 aaaaagcatg ggctgttccg ggtgatcccc attaaagggg catccgtcta cggtaagccg
     2821 gtggccagca tgcctcgtaa gcgaaacaaa aacggggttt accttaccga aatcggtacg
     2881 gataccgcga aagagcagat ttataaccgc ttcacactga cgccggaagg ggatgaaccg
     2941 cttcccggtg ccgttcactt cccgaataac ccggatattt ttgatctgac cgaagcgcag
     3001 cagctgactg ctgaagagca ggtcgaaaaa tgggtggatg gcaggaaaaa aatactgtgg
     3061 gacagcaaaa agcgacgcaa tgaggcgctc gactgcttcg tttatgcgct ggcggcgctg
     3121 cgcatcagta tttcccgctg gcagctggat ctcagtgcac tgctggcgag cctgcaggaa
     3181 gaggatggtg cagcaaccaa caagaaaaca ctggcagatt acgcccgtgc cttatccgga
     3241 gaggatgaat gacgcgacag gaagaacttg ccgctgcccg tgcggcactg catgacctga
     3301 tgacaggtaa acgggtggca acggtacaga aagacggacg gcgagtggag tttacggcca
     3361 cttccgtgtc tgacctgaaa aaatacattg cggagctgga ggtgcagacc ggcatgacac
     3421 agcgacgcag gggacctgca ggattttatg tatgaaaacg cccaccattc ccacccttct
     3481 gggaccggac ggcatgacat cgctgcgtga atatgccggt tatcacggcg gtggcagcgg
     3541 atttggtggg cagttgcggg cgtggaaccc accgggtgaa agtgtggatg cagccctgct
     3601 gcccaacttt acccgtggca atgcccgcgc agacgatctg gtacgcaata acggctatgc
     3661 tgccaacgcc atccagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca
     3721 tcgcccaagc tggcgctatc tgggcatcgg ggaggaagac gcccgtgcct tttcccgcga
     3781 ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg
     3841 aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga
     3901 actgttcgtt caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg
     3961 gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg
     4021 tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg
     4081 ctatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg
     4141 cgcctcgttc attcacgttt ttgaacccgt ggaggacggg cagacccgcg gtgcaaatgt
     4201 gttttacagc gtaatggagc agatgaagat gctcgacacg ctgcagaaca cgcagctgca
     4261 gagcgccatt gtgaaggcga tgtatgccgc caccattgaa agtgagctgg atacgcagtc
     4321 agcgatggat tttattctgg gcgcgaacag taaggagcag cgggaaaggc tgacgggctg
     4381 gattggtgaa attgccgcgt attacgccgc agcaccggtc cgtctgggag gcgcaaaagt
     4441 gccgcacctg atgccggggg actcactgaa cctgcagacg gctcaggaca cggataacgg
     4501 ctactccgtg tttgagcagt cactgttgcg gtatatcgct gccgggctgg gtgtctcgta
     4561 tgagcaactt tcccggaatt acgcccagat gagctactcc acggcacggg ccagtgcgaa
     4621 cgagtcgtgg gcgtacttta tggggcggcg aaaattcgtc gcatcccgtc aggcgagcca
     4681 gatgtttctg tgctggctgg aagaggccat cgttcgccgc gtggtgacgt taccttcaaa
     4741 agcgcgcttc agctttcagg aagcccgcag tgcctggggg aactgcgact ggataggctc
     4801 cggtcgtatg gccatcgatg gtctgaaaga agttcaggaa gcggtgatgc tgatagaagc
     4861 cggactgagc acctacgaga aagagtgcgc gaaacgcggt gacgactatc aggaaatttt
     4921 tgcccagcag gtccgtgaaa cgatggagcg ccgcgcagct ggtcttaaac cgcccgcctg
     4981 ggcggctgcg gcatttgaat ccgggctgcg acaatcaaca gaggaggaga agagtgacag
     5041 cagagctgcg taatctcccg catattgcca gcatggcttt taatgagccg ctgatgcttg
     5101 aacccgccta tgcgcgggtt ttcttttgtg cgcttgcagg ccagcttggg atcagctgcc
     5161 tgacggatgc agtatccggc gacagcctga ctgccggaga ggcacccgcg gcgctggcgt
     5221 tatccggtga tgatgacgga ccacgacagg cccgcagtta tcaggtaatg aacggcatcg
     5281 ccgtgctgcc ggtgtccggc acgctggtca gccggacgcg ggcgctgcag ccgtactcgg
     5341 ggatgaccgg ttacaacggc attatcgcct gtctgcaaca ggctgccagc gacccgatgg
     5401 tggacggcat tctgctcgat atggacacac cgggcggaat ggtggcggga gcatttgact
     5461 gcgctgacat catcgcccgt gtgcgtgaca taaagccggt atgggcgctg gccaatgaca
     5521 tgaactgcag cgcaggtcag ctgcttgcca gtgccgcctc ccggcgtctg gtcacgcaga
     5581 ccgcccggac aggctccatc ggcgtcatga tggctcacag taattacggc gctgcactgg
     5641 agaaacaggg cgtggaaatc acgctgattt acagcggcag ccataaggtg gatggcaacc
     5701 cctacagcca tctaccggat gatgtccggg aaacattgca gtcccggatg gatgcaaccc
     5761 gccggatgtt tgcacagaag gtgtcggcat ataccggcct gtccgtgcag gctgtgctgg
     5821 ataccgaggc tgcagtgtac agcggtcagg aggccattga tgccggactg gctgatgaac
     5881 ttgttaacag taccgatgcg atcaccgtca tgcgtgatgc actggatgca cgtaaatccc
     5941 gtctctcagg agggcgaatg accaaagaga ctcaatcaac aactgtttca gccactgctt
     6001 cgcaggctga cgttactggc gtggtgcaag cgacggaggg cgagaacgcc agcgcggcgc
     6061 agccggacgt gaacgcgcag atcaccgcag cggttgcggc agaaaacagt cgcattatgg
     6121 ggatcctcaa ctgtgaggag gctcacggac gcgaagaaca ggcacgcgtg ctggccgaaa
     6181 cccccggtat gaccgtggaa acggcccgcc gtattctggc cgcagcacca cagagtgcac
     6241 agacgcgcag tgacactgcg ctggatcgtc tgatgcaggg ggcaccggca ccgctggctg
     6301 caggtaaccc ggcatctgat gccgttaacg atttgctgaa cacaccagtg taagggatgt
     6361 ttatgacgag caaagaaacc tttacccatt accagccgct gggcaatagt gacccggctc
     6421 ataccgcaac cgcgcccggc ggattgagtg cgaaagcgcc tgcaatgacc ccgctgatgc
     6481 tggacacctc cacccgtaag ctggttgcgt gggatggcac caccgacggt gctgccgttg
     6541 gcattcttgc ggttgctgct gaccagacca gcaccacact gacgttctac aagtccggca
     6601 cgttccgtta tgaggatgtg ctctggccgg aggctgccag cgacgagacg aaaaaacgga
     6661 ccgtgtttgc cggaacggca atcagcatcg tttaacttta cccttcatca ctaaaggccg
     6721 cctgtgcggc tttttttacg ggattttttt atgtcgatgt acacaaccgc ccagctgctg
     6781 gcggcaaatg agcagaaatt taagtttgat ccgctgtttc tgcgtctctt tttccgtgag
     6841 agctatccct tcactacgga gaaagtctat ctctcacaaa ttccgggact ggtaaacatg
     6901 gcgctgtacg tttcgccgat tgtttccggt gaggttatcc gttcccgtgg cggctccacc
     6961 tctgaattta cgccgggata tgtcaaaccc aagcatgagg tgaatccgca gatgaccctg
     7021 cgtcgcctgc cggatgaaga tccgcagaat ctggcggacc cggcttaccg ccgccgtcgc
     7081 atcatcatgc agaacatgcg tgacgaagag ctggccattg ctcaggtcga agagatgcag
     7141 gcagtttctg ccgtgctcaa gggcaaatac accatgaccg gtgaagcctt cgatccggtt
     7201 gaggtggata tgggccgcag tgcggcgaac aacatcacac agtccggcgt cacggagtgg
     7261 agcaagcgtg acaagtccac gtatgacccg accgacgata tcgaagccta cgcgctgaac
     7321 gccagcggtg tggtgaatat catcgtgttt gacccgaaag gctgggcgct gttccgttcc
     7381 ttcaaagccg tcaaggagaa gctggatacc cgtcgcggct ctcattccga gctggagaca
     7441 gcggtaaaag acctgggcaa agcggtgtcc tataagggaa tgtatggcga tgtggccatc
     7501 gtcgtgtatt ccggacagta cgtggaaaac ggcgtcaaaa agaacttcct gccggacaac
     7561 acgatggtgc tggggaacac tcaggctcgc ggtctgcgca cctatggctg cattcaggat
     7621 gcggacgcac agcgcgaagg cattaacgcc tctgcccgtt acccgaaaaa ctgggtgacc
     7681 accggcgatc cggcgcgtga gttcaccatg attcagtcag caccgctgat gctgctggct
     7741 gatcctgatg agttcgtgtc cgttcaactg gcgtaatcat ggcccttcgg ggccattttc
     7801 tctctgtgga ggagttcatg acgaaagatg aactgattgc ccgtcttcag gagctgggtg
     7861 agcaattgaa ccgcgatgtc agtttgacgg gaacgaaaga agaactggcg cttcgtgtgg
     7921 cagagctgga agaagagctt gatgacacgg atgaaactgc cggtcaggac acccctctca
     7981 gccgggaaaa tgtgctgacc gggcatgaaa atgaggtggt atcagcgcag ccggataccg
     8041 tgattcagga tacggctgaa ctggtcacgg tcgtggcact ggtgacgctg catactgatg
     8101 cacttcacgc cacgcgggat aaacctgtgg catttgtgct gccgggaacg gcgtttcgtg
     8161 tctctgccgg tgtggcagcc gaaatgacag agcgcggcct ggccagaatg caataacggg
     8221 aggcgctgtg gctgatttcg ataacctgtt cgatgctgcc attgcccgcg ccgatgaaac
     8281 gatacgcggg tacatgggaa cgtcagccac catgacatcc ggtgagcagt caggtgcggt
     8341 gatacgtggt gtttttgatg accctgaaaa tatcagctat gccggacagg gcgtgcgcgt
     8401 tgaaggctcc agcccgtccc tgtttgtccg gactgatgag gtgcggcagc tgcggcgtgg
     8461 agacacgctg accatcggtg aggaaaattt ctgggtagat cgggtttcgc cggatgatgg
     8521 cggaagttgt catctctggc ttggacgggg cgtaccgcct gccgttaacc gtcgccgctg
     8581 aaagggggat gtatggccat aaaaggtctt gagcaggccg ttgaaaacct cagccgtatc
     8641 agcaaaacgg cggtgcccgg tgccgccgca atggccatta accgcgttgc ttcatccgcg
     8701 atatcgcagt ctgcgtcaca ggttgcccgt gagacaaagg tacgccggaa actggtaaag
     8761 gaaagggcca ggctgaaaag ggccacggtc aaaaatccgc aggccagaat caaagttaac
     8821 cggggggatt tgcccgtaat caagctgggt aatgcgcggg ttgtcctgtc ccgccgcagg
     8881 cgtcataaaa aggggcagcg ttcatccctg aaaggtggcg gcagcgtgct tgtggtgggt
     8941 aaccgtcgta ttcccggcgc gtttattcag caactgaaaa acggccggtg gcatgttatg
     9001 cagcgtgtgg ccgggaaaaa ccgttacccc attgatgtgg tgaaaatccc gatggcggtg
     9061 ccgctgacca cggcgtttaa acagaatatt gaacggatac ggcgtgaacg tcttccgaaa
     9121 gagctgggct atgcgctgca gcatcaactg agaatggtaa taaagcgatg aaacatactg
     9181 aactccgtgc agccgtactg gatgcactgg agaagcatga caccggggcg acgctttttg
     9241 atggtcgccc cgctgttttt gatgaggaag attttccggc aattgccgtt tatctcaccg
     9301 gcgctgaata cacgggcgaa gagctggaca gcgatacctg gcaggcggag ctgcatattg
     9361 aagttttcct gcctgctcag gtgccggatt cagagctgga ttcgtggatg gagtcccgga
     9421 tttatccggt gatgagcgat atcccggcac tgtcagattt gatcaccagt atggtggcca
     9481 gcggctatga ctaccggcgc gacgatgatg cgggcctgtg gagttcagcc gatctgactt
     9541 atgtcattat ctatgaaatg tgaggacgct atgcctgtac caaatcctac aatgccggtg
     9601 aaaggtgccg ggaccaccct gtgggtttat aaggggagcg gtgaccctta tgcgaacccg
     9661 ctttcagacg ttgactggtc gcgtctggca aaagttaaag acctgacgcc cggcgaactg
     9721 accgctgagt cctatgacga cagctatctc gatgatgaag atgcagactg gactgcgacc
     9781 gggcaggggc agaaatctgc cggagatacc agcttcacgc tggcgtggat gcccggagag
     9841 caggggcagc aggcgctgct ggcgtggttt aatgaaggtg atacccgtgc ctataaaatc
     9901 cgcttcccga acggcacggt cgatgtgttc cgtggctggg tcagcagtat cggtaaggcg
     9961 gtgacggcga aggaggtgat tacccgcacg gtgaaggtca ccaatgtggg acgtccgtcg
    10021 atggcagaag atcgcagcac ggtgacggcg acgaccggca tgacggtgac acccgccagt
    10081 gcttccgtag tgaaagggca gagcaccacg ctgaccgtgg cattccagcc ggatggcgca
    10141 accgacaaga gcttccgtgc ggtgtctgcg gataaaacaa aagccaccgt gtcggtcagt
    10201 ggtatgacca tcaccgtgaa aggtgttgct gcaggcaagg tcaacattcc ggtcgtatct
    10261 ggtaatggtg agtttgctgc ggttgcagaa atcaacgtca ccgccagtta atccggagag
    10321 tcagcgatgt tcctgaaaac cgaatcattt gaacataacg gtgtgaccgt cacgctttct
    10381 gaactgtcag ccctgcagcg tattgagcat ctcgacctga tgaaacggca ggcagaacag
    10441 gcggagtcag acagcaaccg gaagtttact gtggaagacg ccatcagaac cggcgctttt
    10501 gtggttgcga tgtccctgtg gcataaccat ccgaagaaga cgcagatgcc gtccatgaat
    10561 gaagccgtta aacagattga gcaggaagtg cttaccacct ggcccacaga ggcaatttct
    10621 catgctgaaa acgtggtgta ccggctgtcc ggtatgtatg agtttgtggt gaatgatgct
    10681 cctgaacagg cagaggacgc cgggcctgca gagcctgttt ctgcgggaaa gtgttcgacg
    10741 gtgagctgag ttttgccctg aaactggcgc gtgagatggg gcgacccgac tggcgcgcca
    10801 tgcttgccgg gatgtcatcc acggagtatg ccgactggca ccgcttttac agtacccatt
    10861 attttcatga tgttctgctg gatatgcact tttccgggct gacgtacacc gtgctcagcc
    10921 tgtttttcag cgatccggag atgcatccgc tggatttcag tctgctgaac cggcgcgagg
    10981 ctgacgaaga gcctgaagat gatgtgctga tgcagaaagc ggcagggctt gccggaggtg
    11041 tccgctttgg cccggacggg aatgaagtta tccccgcttc cccggatgtg gcggacatga
    11101 cggaggatga cgtaatgctg atgacagtat cagaagggat cgcaggagga gtccggtatg
    11161 gctgaaccgg taggcgatct ggtcgttgat ttgagtctgg atgcggccag atttgacgag
    11221 cagatggcca gagtcaggcg tcatttttcc ggtacggaaa gtgatgcgaa aaaaacagcg
    11281 gcagtcgttg aacagtcgct gagccgacag gcgctggctg cacagaaagc ggggatttcc
    11341 gtcgggcagt ataaagccgc catgcgtatg ctgcctgcac agttcaccga cgtggccacg
    11401 cagcttgcag gggggcagaa tccctggctg atcctgctgc aacagggtgg tcaggttaag
    11461 gactccttcg gcgggatgat ccccatgttc agggggcttg ccggcgcgat caccctgccg
    11521 atggtggggg ccacctcgct ggcggtggcg accggtgcgc tggcgtatgc ctggtatcag
    11581 ggcaactcaa ccctgtccga tttcaacaaa acgctggtcc tttccggtaa tcaggcggga
    11641 ctgacggcag atcgtatgct ggtcctgtcc agagccgggc aggcggcagg gctgacgttt
    11701 aaccagacca gcgagtcact cagcgcactg gttaaggcgg gggtaagcgg tgaggctcag
    11761 attgcgtcca tcagccagag tgtggcgcgt ttctcctctg catccggcgt ggaggtggac
    11821 aaggtcgctg aagccttcgg gaagctgacc acagacccga cgtcggggct gacggcgatg
    11881 gcacgccagt tccataacgt gacggcggag cagattgcgt atgttgctca gttgcagcgt
    11941 tccggcgatg aagccggggc attgcaggcg gcgaacgagg ccgcaacgaa agggtttgat
    12001 gaccagaccc gccgcctgaa agagaacatg ggcacgctgg agacctgggc agacaggact
    12061 gcacgtgcat tcaaatccat gtgggatgcg gtgctggata ttggtcgtcc tgataccgcg
    12121 caggagatgc tgattaaggc agaggctgcg tttaagaaag cagacgacat ctggaatctg
    12181 cgcaaggatg attatttcgt taacgatgaa gcacgggcgc gttactggga tgatcgtgaa
    12241 aaaaaacgcc tggagcggga tgcagcccaa aagagggtag atcaacagcg tcaacaggac
    12301 aaaaatgcgc agcagcagag cgataccgaa gcgtcacggc tgaaatatac cgaagaggcg
    12361 cagaaggctt acgaacggct gcagacgccg ctggagaaat ataccgcccg tcaggaagaa
    12421 ctgaacaagg cactgaaaga cggaaaaatc ctgcaggcag attacaacac gctgatggcg
    12481 gcggcgaaaa aggattatga agcgacgctg aaaaagccga aacagtccgg cgtgaaggtg
    12541 tatgcgggcg atcgtcagga agacagtgct catgcggccc tgctgacgct tcaggcagaa
    12601 ctccggacgc tggagaagca tgccggagcg aatgagaaaa tcagccagca gcgccgggat
    12661 ttgtggaagg cagaaagtca gttcgcggta ctggaggagg cggcacaacg tcgccagctg
    12721 tccgcacagg agaaatccct gctggcgcat aaagacgaga cgctggagta caaacgccag
    12781 ctggctgcac ttggtgacaa ggttacgtat caggagcacc tgaatgcgct ggcgcagcag
    12841 gcggataaat tcgcacagca gcaacgggca aaacgggcag ccattgatgc gaaaaaccgg
    12901 gggctgactg accggcaggc agcgcgggaa gccacggaac agcgcctgaa ggaacagtat
    12961 ggcgataatc ctctggcgct gaataacgtc atgtcagagc agaaaaagac ctgggcggct
    13021 gaagaccagc ttcgcgggag ctggatggca ggcctgaagt ccggctggag tgagtgggaa
    13081 gagagcgcca cggacagtat gtcacaggtt aaaagtgcag ccacgcagac ctttgatggt
    13141 attgcacaga atatggcggc gatgctgacc ggcagtgaac agaactggcg cagcttcacc
    13201 cgctccgtgc tgtccatgat gacagaaatt ctgcttaagc aggcaatggt ggggattgtc
    13261 gggagtatcg gcagcgccat tggcggggct gttggtggcg gcgcatccgc gtcaggtggt
    13321 acagccattc aggccgctgc ggcgaaattc cattttgcaa ccgggggatt tacgggaacc
    13381 ggcggcaaat atgagccagc ggggattgtt caccgtggtg aatttgtctt cacgaaggag
    13441 gcaaccagcc ggattggcgt ggggaatctc taccggctga tgcgcggcta tgcggaaggt
    13501 ggttatgtgg gcggtgccgg aagtccggcg cagatgcggc gggctgaagg cattaatttt
    13561 aatcagaaca atcacgtggt gattcagaac gacggtacga atggtctgcc aggtccacag
    13621 atgatgaagg ccgtgtatga catggcccgc aagggtgccc gtgatgaaat tcagacacag
    13681 atgcgtgatg gtggcctgtt ctccggaggt ggacgatgaa aaccttccgc tggaaagtga
    13741 aacccggtat ggatgtggct tcggcccctt ctgtaagaaa ggtgcgcttt ggtgatggct
    13801 attcccagcg agcgcctgcc gggctgaatg ccaacctgaa aacgtacagc gtgacgcttt
    13861 ctgtcccccg tgaggaggcc atggtactgg agtcgtttct ggaagagcac gggggctgga
    13921 aagcctttct gtggacgccg ccttatgagt ggcggcagat aaaggtgacc tgcgcaaaat
    13981 ggtcgtcgcg ggtcagtatg ctgcgtgttg agttcagcgc agagtttgaa caggtggtga
    14041 actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcggc
    14101 cagcgtggtg ctctgggaaa tcgatctgac agaggtcggt ggagaacgtt attttttctg
    14161 taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt atcagccgta
    14221 tcccattcag gggagtggtt ttgaactgaa tggcaaaggc accagtacgc gccccacgct
    14281 gatggtttct aacctgtacg gtatggtcac cgggatggcg gaagatctac agagtctggt
    14341 cggcggaacg gtggtccggc gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt
    14401 caacggaaac agtgacgccg atccggagca ggaggtgatc agccgctggc gcattgagca
    14461 gtgcagcgaa ctgagcgcgg tgagtgcctc ttttgtactg tccacgccga cggaaacaga
    14521 tggcgctgtt tttccgggac gtatcatgct ggccaacacc tgcacctgga cctatcgcgg
    14581 cgatgagtgc ggttatcacg gtccggcggt cgcggatgaa tatgaccagc caacgtccga
    14641 catcacgaag gataaatgca gcaaatgcct gagtggctgt aagtttcgca ataacgtcgg
    14701 caactttggc ggcttccttt ccattaacaa actttcgcag taaatcccat gacagagaca
    14761 gaatcagcga ttctggcgca cgcccggcga tgtgctccag cggagtcgtg cggcttcgtg
    14821 gtaagaacgc cggaggggga aagatatttc ccctgcgtga atatctccgg tgagccggag
    14881 gcgtatttcc gtatgtcgcc ggaagactgg ctgcaggcag agatgcaggg tgagattgtg
    14941 gcactggtcc acagccaccc cggtggtctg ccctggctga gtgaggccga ccggcggctg
    15001 caggtgcaga gtgatttgcc gtggtggctg gtctgccggg gggcgattca caagttccgc
    15061 tgtgtgccac atcttaccgg gcggcgcttt gagcacgggg tgacggactg ttacacgctg
    15121 ttccgggatg cttaccatct ggcggggatt gagatgccgg attttcatcg tgaggatgac
    15181 tggtggcgtc acggtcagaa tctctatctg gataatctgg aggccacagg gctgtatcag
    15241 gtgccgttgt cagcggcgca gccgggcgat gtgctgctgt gctgttttgg ttcatcggtg
    15301 ccgaatcatg ccgccattta ctgtggtgac ggcgagctgc tgcaccatat tcctgaacaa
    15361 ctgagcaaac gagagaggta taccgacaaa tggcagcgac gcacacactc cctctggcgt
    15421 caccgggcat ggcacgcatc tgcctttacg gggatttgca acgatttggc cgccgcatcg
    15481 accttcgtgt gaaaacgggt gccgaagcca tccgggcgat ggccacacag ctccctgcgt
    15541 ttcgtcagaa gctgagcgac ggctggtatc aggtgcgtat tgccgggcag gatgttagca
    15601 cgtccggatt aacggcgcag ttacatgagg ctctgcctga cggcgctgtg attcatattg
    15661 ttcccagagt cgccggggcc aagtcaggtg gtgtattcca gattgtcctg ggagcagccg
    15721 ccattgccgg atcattcttt accgccggag ccacccttgc agcatggggg gctgccattg
    15781 gtgccggtgg tatgaccggc attctgtttt ctctcggtgc cagtatggtg ctcggcggtg
    15841 tggctcagat gctggcaccg aaagccagaa ctccccgcac acagacaacg gataacggta
    15901 agcagaacat ctatttctcc tcactggata acatggttgc ccagggcaat gttatgcctg
    15961 ttctgtatgg tgaaatgcgc gtggggtcac gtgtggtatc tcaggagatc agcacggcag
    16021 acgaagggga cggtggtcag gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga
    16081 aatcgcctcc gggcggtttt atcgtttatg gagcatgacg aatgggtaaa ggcagcagta
    16141 aggggcatac cccgcgcgaa gcgaaggaca acctgaagtc cacgcagctg ctgagtgtga
    16201 tcgatgccat cagcgaaggg ccggttgaag gtccggtgga tggattaaaa agcgtgctgc
    16261 tgaacagtac gccggtgctg gacagtgagg ggaataccaa catctccggc gtcacggtgg
    16321 tgttccgggc aggtgagcag gagcagtcac cgccggaggg atttgaatcc tccggctccg
    16381 agacggtgct gggtacggaa gtgaaatatg acacgccgat cacccggacc atcacgtcgg
    16441 caaacattga ccgactgcgt tttaccttcg gtgtacaggc actggtggaa accacctcaa
    16501 agggagacag gaatccgtct gaagttcgcc tgctggttca gatacagcgt aacggtggct
    16561 gggtgacgga aaaagacatc accattaagg gcaaaaccac ctcgcagtat ctggcctcgg
    16621 tggtggtgga taacctgccg ccgcgcccgt ttaatatccg gatgcgcaga atgacgccgg
    16681 acagcaccac agaccagctg cagaacaaaa cgctctggtc gtcatacacc gaaatcatcg
    16741 atgtgaaaca gtgctacccg aacacggcac tggtcggcgt acaggtggat tcggagcagt
    16801 tcggcagcca gcaggtgagc cgtaattatc atcttcgcgg gcgcattctg caggtgccgt
    16861 cgaactataa tccgcagacg cggcaataca gcggtatctg ggacggaacg ttaaaaccgg
    16921 catacagcaa caacatggcc tggtgtctgt gggatatgct gacccatccg cgctatggca
    16981 tggggaaacg tcttggtgcg gcagatgtgg ataaatgggc gctgtatgtc atcggccagt
    17041 actgtgacca gtcggtgccg gacggctttg gcagcacgga gccgcgcatc acctgtaatg
    17101 cctggctgac cacacagcgc aaggcgtggg atgtgctcag tgatttctgc tcggcgatgc
    17161 gctgtatgcc ggtatggaac gggcagacgc tgacgttcgt gcaggaccga cagtcggata
    17221 aggtgtgggc ctataaccgc agtaatgtgg tgatgccgga tgatggcgcg ccgttccgct
    17281 acagtttcag cgccctgaaa gaccgccata atgccgttga ggtgaactgg attgacccgg
    17341 ataacggctg ggagacggcg acagagcttg tggaggatac gcaggccatt gtccgttacg
    17401 gtcgtaacgt cacgaagatg gatgcctttg gctgtaccag ccgggggcag gcacaccgcg
    17461 ccgggctgtg gctgattaaa acggaactgc tggagacgca gaccgtggat ttcagcgtgg
    17521 gtgctgaagg gcttcgtcat gtaccgggcg atgtcattga aatctgcgat gatgactatg
    17581 ccggtatccg caccggcggg cgcgtgctgg cggtgaacag ccagacccgg acgctgacgc
    17641 tcgaccgtga aatcacgctg ccatcctccg gcaccacgct gataagcctg gttgacgggc
    17701 aggggaatcc ggtcagcgtg gaggtccagt ccgtcaccga cggcgtgaag gtaaaagtga
    17761 gccgtgttcc tgacggtgtt gctgaataca gcgtgtgggg gctgaagctg ccgacgctgc
    17821 accagcgatt gttccgctgc gtgagtatcc gtgagaacga cgacggcacg tatgccatca
    17881 ccgccgtgca gcatgtgccg gaaaaagaag ccatcgtgga taacggggcg cactttgacg
    17941 gcgaccagag cggcacggtg aatggtgtca cgccgccagc ggtgcagcac ctgactgccg
    18001 aagtcaccgc agacagcggg gaatatcagg tgctggcgcg ctgggacacg ccgaaggtgg
    18061 tgaagggcgt gagcttcctg ctccgtctga ccgtaacagc ggatgacggc agtgagcggc
    18121 tggtcagcac ggcccggacg acggaaacca cttaccgctt cacacaactg gcgctgggga
    18181 actacaggct gacagtccgg gcagtaaatg cgtgggggca gcagggcgat ccggcgtcgg
    18241 tatcgttccg gattgccgca ccggcagcgc tgtcgcggat tgagctgacg ccgggctatt
    18301 ttcagataac cgccacgccg catcttgcgg tttatgatcc gacggtacag tttgagttct
    18361 ggttctcgga aaagcggatt gcggatatca ggcaggttga aaccacagca cgctatcttg
    18421 gcacggcgct gtactggata gccgccagta tcaatatcaa accgggccat gattattact
    18481 tttatatccg cagtgtgaac accgttggca aatcggcatt cgtggaggct gttggtcagc
    18541 cgagtgatga tgcatccggt tatctggatt ttttcaaagg cgagataggg aaaacccatc
    18601 tggctcagga gctgtggacg cagattgata acggtcagct tgcgcctgac ctggctgaaa
    18661 tcaggacgtc cattacggat gtcagcaatg aaatcacgca gaccgtcaat aagaaactgg
    18721 aagaccagag tgcggcaatt cagcagatac agaaggttca ggttgataca aataataacc
    18781 tgaacagcat gtgggctgtg aagctgcagc agatgcagga cggacgcctt tatatcgcgg
    18841 gtattggtgc cggtattgag aacacccctg acggcatgca gagtcaggtg ctgctggcgg
    18901 cggacaggat tgcgatggtt aatcctgcga atggcaacac aaaaccgatg tttgttggtc
    18961 agggcgatca gatattcatg aacgacgtgt tcctgaaacg cctgacggcc cccaccatta
    19021 ccagcggtgg aaatccatcg gcattttccc tgactccgga cggaaagctg accgctaaaa
    19081 atgcggatat cagtggcagt gtgaatgcga acgccgggac gctcaacaat gtcacggtaa
    19141 atgaaaactg tacgattaag ggcatgctgg aggcgactca ggtcagaggt gacttcgtta
    19201 aagctgtatc caaatcattt ccgaaacagg ctggtacgtg gggtaacacg gaaacaccaa
    19261 acgggacggt tacagtcacc ataagcgatg atcataactt tgaccgccag attattattc
    19321 cgcccattat ttttaacggt atagcgtatg acgatccggg gagcggaaat aacccaggag
    19381 gcacgcgata cacgggttat ggttttgaag ttcgcaaaaa cggcgtatta atcgcatcca
    19441 gagaaactaa aggtgccatt cccggtagtt acagtgcagt tattgatatg cctagtggtg
    19501 gtggtagcgt cactctggag tttaagattt tccagaaagg caatcagggg gcaggcaata
    19561 tcaccgactg tacggtgatt gtgaccaaaa aagcggcttc cggcatcagt attcgttgaa
    19621 atatttataa ccccaataaa tggcgtcagg aatgacgcct tttttattgc agaaaagcga
    19681 gaggtaatta tgcgtaaagt ttgtgcagcc attttgtccg cagccatttg cctggccgta
    19741 tccggtgcgc ctgcatgggc gtctgaacat cagtccacgc tgagcgcggg gtatcttcat
    19801 gcccggacca acgttcccgg cagtgatgat ctgaacggga ttaacgtgaa ataccgttat
    19861 gagtttacgg atacgctggg gctggtgacg tcattcagct atgcaggaga caagaatcgc
    19921 cagctgaccc gttacagcga tacccgctgg catgaagatt ccgtgcgtaa ccgctggttc
    19981 agcgtgatgg cggggccgtc tgtgcgcgtg aatgaatggt tcagcgcgta tgcgatggcg
    20041 ggtgtggctt acagccgtgt gtcgactttt tccggggatt atatccgcgt aactgacaat
    20101 aaggggaaaa cgcacgatgt gctgaccgga agtgatgacg gtcgccacag caacacgtct
    20161 ctggcgcact gacggtttca tcgtgggtgt cggttataaa ttctgattag ccaggtaaca
    20221 cagtgttatg acagcccgcc ggttcaggcg ggcttttttg tggggtgaat atggcagtaa
    20281 agatttcagg tgtactgaaa gacggcacag gaaaaccggt acagaactgc acaatccagc
    20341 tgaaagcaaa acgtaacagc accacggtgg tggtgaacac gctggcctca gaaaatccgg
    20401 atgaagccgg gcgttacagc atggacgttg agtacggtca gtacagcgtt attctgttgg
    20461 tggaaggatt cccgccgtca catgccggga ccatcaccgt gtatgaagat tcccgacccg
    20521 gtacgctgaa tgattttctc ggtgccatga cggaggatga tgcccgtccg gaggcactgc
    20581 gccgttttga gctgatggtg gaagaggtgg cgcgtaacgc gtccgcggtg gcacagaaca
    20641 cggcagccgc gaagaaatca gccagcgatg ccagcacatc agcccgtgag gcggcaaccc
    20701 atgcgactga tgctgcagat tcagcacgtg cagccagcac gtcagccgga caggccgctt
    20761 cgtcggctca gtcagcgtct tccagcgcag gaacggcatc aacaaaggcc actgaagcct
    20821 caaaaagtgc tgccgctgca gagtcctcaa aaagcgcggc ggctaccagt gccggtgcgg
    20881 cgaaaacgtt agaaacgaat gcggcagcgt cacaacaatc agcagccact tctgcatcca
    20941 ccgcgaccac gaaagcgtca gaagctgcca cctcagcccg ggatgcgtcg gcttcaaaag
    21001 aggcggcaaa atcatcagaa acgaacgcat catcaagcgc cagtagcgca gcttcctcgg
    21061 cgacggcggc tgcaaattct gcgaaggcgg ccaaaacgtc cgagacgaac gccaggtctt
    21121 ctgaaacggc agcgggacag agtgcctcag ctgcggcagg ctcaaaaaca gcggctgcgt
    21181 cgtctgccag cgccgcgtca acaagtgccg ggcaggcctc agccagtgcc accgccgccg
    21241 gaaaatcggc agaaagcgcc gcatcatccg cttcaacagc cacaacgaag gctggcgaag
    21301 ccactgagca ggccacggca gcagcgaggt cagcttccgc agcgaagaca tccgaaacga
    21361 acgcgaaagc gtcggaaacc cgtgcagaat cctcaaaaac ggctgccgca tcgtccgcca
    21421 gttcggcggc gtcatcggca tcatcggcgt ctgcttcaaa agatgaggcg accagacagg
    21481 cgtcagcagc gaagggcagc gccacgacgg catccacgaa ggcgacagag gctgctggta
    21541 gtgcgacggc agcagctcag agcaaaagta cggcggaatc tgcagcaacg cgcgctgaga
    21601 cagcggcaaa acgggcagag gatattgcat ccgccgtggc gcttgaggat gcgagcacga
    21661 cgaaaaaggg gatagtacag ctcagtagtg cgactaacag cacttccgag tcactggcgg
    21721 caacaccaaa agccgttaag gccgcgtatg acctggctaa cgggaaatac accgcacagg
    21781 atgcaacgac agcacagaaa gggataatcc agctaagcag cgcgaccaac agcacgtctg
    21841 aaacgctggc ggcaacgcca aaggcagtaa aagcagccaa tgacaatgct gagaaacgtc
    21901 tgcagaaaga tcagaacggt gcggatatcc ctggcaaaga cacctttacg aaaaatattg
    21961 gtgcctgccg tgccttcggt gggtcagtaa gcacaacaac aggaaactgg acgactgcac
    22021 agtttatcga gtggctggat tctcagggag catttaacca tccatactgg atgtgcaagg
    22081 gttcctggtc ttatggcaat aataaaatca ttactgatac tggctgcggt aatattcatc
    22141 tcgccggagc tgtcattgaa gtaatgggga taaagtcagc gatgacgatc cgcattacca
    22201 ctccgaccac atccagcggt ggcggaacaa ccaacgccca gtttacctat attaatcacg
    22261 gaacagatta ttcacctggc tggcgaaggg actataactc cagaaataag ccaacggcat
    22321 cagagatcgg ggcgctaccg tcaggtggaa cagcagtatc atcagttaat ctggcttcaa
    22381 aaggtcgggt agccgcgctg acagacaata cgcagggggc agcaggtctt gagttatacg
    22441 aggtgtataa caacggatat ccaacagcgt atggaaatat cattcacctg aaagggatga
    22501 cagccgttgg cgaaggtgag ttactcatcg gctggagtgg tacaagcggt gctcatgctc
    22561 cggcatttat tcgttcacga cgggatacga ccgacgcaaa ctggtcgccg tgggcgcagc
    22621 tttacacctc ggctcatcct cctgcagagt tttatccagt cggtgcacca atcccgtggc
    22681 catcagatac cgttccgtct ggttatgccc tgatgcaggg gcagactttt gacaaatctg
    22741 cttacccgaa acttgcagcc gcttatccgt caggcgtgat ccctgatatg cgtggctgga
    22801 cgattaaggg caagcccgcc agtggtcgtg ccgtattgtc tcaggaacag gatggcatta
    22861 aatcgcacac ccacagcgcc agcgcatcca gtacggattt ggggacgaaa accacatcgt
    22921 cgtttgatta cggcactaaa tccacgaata acaccggggc gcatacgcac agtctgagcg
    22981 gctctacaag ttcagctggg gcccaccaac atagtcagac tgggcccaga acgaatagcg
    23041 ggagtcaacc tactggaatg tttccggcag ggagtactca agttagcggg actaatcagg
    23101 ttggtatcag tggctcatta accagtggca cctcccaatg ggtaggtaaa tcatcttcag
    23161 aaggaaacca tacacactca ctgtccggca cagcagcatc tgcgggtgca cacgcgcata
    23221 ctgtcggtat tggtgctcat acgcactctg ttgcgattgg ttcacatgga cacaccatca
    23281 ccgttaacgc tgctggtaac gcggaaaaca ccgtcaaaaa catcgcattt aactatattg
    23341 tgaggctcgc ataatggcat tcagaatgag tgaacaagca cggaccataa aaatttataa
    23401 cctgctggcc ggaactaatg aatttattgg tgaaggtgac gcatatattc cgcctcatac
    23461 aggtctgcct gcaaacatcg tggcatctcg ttgaagatca tcggggtaaa acggtttatg
    23521 acgtggcttc cggcgacgcg ttatttattt ctgaactcgg ctcattaccg gaaaatgtca
    23581 cctggttatc cccggaaggg gagtttcaga agtggaacgg cacagcctgg gtgaaggata
    23641 cggaagcaga aaaactgttc cggatccggg aggcggaaga aacaaaaaac agcctgatgc
    23701 aggtagccag tgagcatata gcgccgcttc aggatgctgt ggatctggag atcgcaacgg
    23761 agaaagaaac cttgttgctg gaagcctgga aaaagtatcg ggtgttgctg aaccgtgttg
    23821 atacatcaac tgcaccggat attgaatggc cagtagcacc tatagggtaa attcgtaatg
    23881 attacctaaa tacgttattc ttttgttaaa aaagtgattt tgctataggt agggagtatg
    23941 ttcttggatt aagaataaaa aatcgccatg aaagagcttc atggcgagta cataaacgta
    24001 caaaatgtga tgaaagtaga gtggcgtaag ccactatatt a
//
LOCUS       DAZRUF010000040        23814 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZRUF010000040 DAZRUF010000000
VERSION     DAZRUF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.040"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(51..3932)
                     /gene="entF"
                     /locus_tag="ACHXOL_001295"
     CDS             complement(51..3932)
                     /gene="entF"
                     /locus_tag="ACHXOL_001295"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR6808318.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     TDLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(3929..4147)
                     /gene="ybdZ"
                     /locus_tag="ACHXOL_001296"
     CDS             complement(3929..4147)
                     /gene="ybdZ"
                     /locus_tag="ACHXOL_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR6808319.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4150..5352)
                     /gene="fes"
                     /locus_tag="ACHXOL_001297"
     CDS             complement(4150..5352)
                     /gene="fes"
                     /locus_tag="ACHXOL_001297"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR6808320.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5595..7835
                     /gene="fepA"
                     /locus_tag="ACHXOL_001298"
     CDS             5595..7835
                     /gene="fepA"
                     /locus_tag="ACHXOL_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR6808321.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            8010..8630
                     /gene="entD"
                     /locus_tag="ACHXOL_001299"
     CDS             8010..8630
                     /gene="entD"
                     /locus_tag="ACHXOL_001299"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR6808322.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8752..8904)
                     /gene="hokE"
                     /locus_tag="ACHXOL_001300"
     CDS             complement(8752..8904)
                     /gene="hokE"
                     /locus_tag="ACHXOL_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR6808323.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9255..9407)
                     /gene="hokE"
                     /locus_tag="ACHXOL_001301"
     CDS             complement(9255..9407)
                     /gene="hokE"
                     /locus_tag="ACHXOL_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR6808324.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9376..9644
                     /locus_tag="ACHXOL_001302"
                     /pseudo
     CDS             9376..9644
                     /locus_tag="ACHXOL_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9847..10965
                     /locus_tag="ACHXOL_001303"
     CDS             9847..10965
                     /locus_tag="ACHXOL_001303"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR6808325.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            11031..11279
                     /locus_tag="ACHXOL_001304"
                     /pseudo
     CDS             11031..11279
                     /locus_tag="ACHXOL_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            11344..11712
                     /locus_tag="ACHXOL_001305"
     CDS             11344..11712
                     /locus_tag="ACHXOL_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR6808326.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11806..12459
                     /gene="nfsB"
                     /locus_tag="ACHXOL_001306"
     CDS             11806..12459
                     /gene="nfsB"
                     /locus_tag="ACHXOL_001306"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR6808327.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12567..13814
                     /gene="ybdG"
                     /locus_tag="ACHXOL_001307"
     CDS             12567..13814
                     /gene="ybdG"
                     /locus_tag="ACHXOL_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR6808328.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13882..15258)
                     /gene="pheP"
                     /locus_tag="ACHXOL_001308"
     CDS             complement(13882..15258)
                     /gene="pheP"
                     /locus_tag="ACHXOL_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR6808329.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15360..18503)
                     /gene="cusA"
                     /locus_tag="ACHXOL_001309"
     CDS             complement(15360..18503)
                     /gene="cusA"
                     /locus_tag="ACHXOL_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR6808330.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18515..19738)
                     /gene="cusB"
                     /locus_tag="ACHXOL_001310"
     CDS             complement(18515..19738)
                     /gene="cusB"
                     /locus_tag="ACHXOL_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR6808331.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(19754..20086)
                     /gene="cusF"
                     /locus_tag="ACHXOL_001311"
     CDS             complement(19754..20086)
                     /gene="cusF"
                     /locus_tag="ACHXOL_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR6808332.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20110..21492)
                     /gene="cusC"
                     /locus_tag="ACHXOL_001312"
     CDS             complement(20110..21492)
                     /gene="cusC"
                     /locus_tag="ACHXOL_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR6808333.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21649..22332
                     /gene="cusR"
                     /locus_tag="ACHXOL_001313"
     CDS             21649..22332
                     /gene="cusR"
                     /locus_tag="ACHXOL_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR6808334.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22322..23770
                     /gene="cusS"
                     /locus_tag="ACHXOL_001314"
     CDS             22322..23770
                     /gene="cusS"
                     /locus_tag="ACHXOL_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR6808335.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5750 a         6494 c         6040 g         5530 t
ORIGIN      
        1 aaaaacccat aattattttc tgtaattatg ggtttataga taatattaat ttacctgttt
       61 agcgttgcgc gaataatcgg cccaattttt acaaacgccc ctggagagat aatatccaca
      121 tgcgcacaat cctgacgata gatatccagc tccgcaatcc acggcgacca ggcgcgttca
      181 ggactcatac cttcctgaag cgtgcgttca gcaacaaaca acgtcgcttt accgtcaaac
      241 ggtacgctat gagcagtcgt cagcaggcgc acagcatcag cgtagttgcc ttcaatggtg
      301 gtaaacaact ccgttgaagt acttccctgc tgtgccgcca ggaaggcctc gcgctcgcgg
      361 ttaatctccg ccagcacttc cgggtccaga ccattagctt ctttttcctg ccagttttgc
      421 gtttctggcg gccaggtatc cagcaagcca agaaatgcca cctgttcgcc acgggcacgc
      481 agtcgcgccg caataccctg cgccagcgta ccgccaaggg aataccccag caggtaataa
      541 gggccgtgcg gttgttgctc aagtaacgtt gccagatgcg cttcgcagac ttcatccagg
      601 tttgtcgctg tctgcatggg tccatgaggg cgcggcgact gaatgccaat aatcgaccat
      661 tgtggatcga gataacgcga gagtacgcta aactgccagg caaaaccgga cgccggatgg
      721 aaacaaaaca gcgtcgggcc attaccttca cgcaacggca gaatggtttc gaatcccatg
      781 cgccgggagc tgtcctcttc accatcaata atcgttgcca gtttggcgac ggttgacgcg
      841 accatcacct gccccggcgt cacctgacga gcaaactgtc gacttaactg cgctgccagt
      901 ttcattgcca gtagcgaatg accgcccagc gcaaagaaat cagcatcggc atcctgcacg
      961 tcacaaccca gcaacgacgc gaatgccgcg gcgataatcg tttcactgcc cgctttcggt
     1021 gcacgtcccg acgcttgtgt cttcagttca ggcaacggta aggctttgcg atccagcttg
     1081 ccgttggcgc taagaggtaa ctgtggaagt tgcagcagaa ccaccggtac catatgtggt
     1141 ggcaatgttt cacgaagctg cgcctgcaat gcgctggtat ccaacggcag gcccgattgc
     1201 gacaccagat agcccaccaa ctgacgcgca tcaccaccag tggctgccgc ctggttaatc
     1261 acacaggcgt gggtaacggc ttgttcgaca tccggcagcg cctgcatcac gcgatcgatt
     1321 tcgcccagtt cgatacgctg cccgcgaatt tttagctgat cgtcactgcg cccgaggtac
     1381 tccaccgcgc cgttatccag ccagcgggca acgtctccgg tacggtacat ccgttcgcca
     1441 ggagcaaaag gatcggcaat aaagcggctg gcggtcagat cggggcgtcc gagatagccc
     1501 tgcgccagtt gaatgccggt aagatagaga tcacccgcca cacccggcgg caccggatgc
     1561 atcatcgcat caagaatacg cagccccatg ttccacaccg gataaccaat cggcacactg
     1621 ctgccgcgca cctgtgccag ttcctcgcca aaagccggat accagctgac atctaccgcc
     1681 gcttccgtcg ggccatacag attatgtaac ggcgcaccgg ttaactgttg ccattcacgg
     1741 cataaatcag ccggtaaggc ttcaccacta cagaaaacct gtttcaacgt cgcgcaactc
     1801 tggcgagcgg tttgcggagt cagcgaggca acaaatgccg ccagcatcga cggcacaaag
     1861 tgcgtggtcg ttacgccata ttcggcaaag aattgctgca tagcgagcgg gtcacggtgc
     1921 gcttccggtt cagccatcac cagttttgcc cctgcgataa acggccagaa aaactcccac
     1981 accgagacat caaaactgca cggcgttttt tgggcaacga catcttcgcc tgtaagcggg
     2041 tagtgatttt gcatccataa caggcggtta acgatagccg tctgcccgac cattacccct
     2101 ttcggcctgc cggtggagcc agaggtaaag atgatataag ccgtgtggtg cggttgtgaa
     2161 agttgcagcg gcgcactgcc ctggggggta agcggggcgt tatagcaaag gcttgttaaa
     2221 ttgggaacat cgctaaagcg cggcagttga tcgtcggtgg taattaacag cgacggacgc
     2281 gcatcttcca gcatcatttt caggcgatcg tccggatagc cggtatccag cggtagccag
     2341 gccgcgccag cttcaactat tgcatggagt gccagggtca aaaagaccga gcgcggtagt
     2401 gccaccgcca cgctgtcccc tggtttaacg ccgcgctcac gcagcagatt cgccagcgcc
     2461 accacctgct cgcgcatttc ccgatagctg aacaggtaac gcgcatctgc cagcgccgga
     2521 gcatccggtg tttttgccgc ttgttctgcc accagcgcgc tcagcgtggt ttctggaatc
     2581 tcaacctgag tggcgttgat ctgcgccagc tgcgcatact cacctggcag cataatatcg
     2641 acatcgccgc acaacagcgc cggatccgcg gcgaactggg caatcagcat tttcaggcgt
     2701 tcagcatgct ggattaacgt tggctcatcg taacgctgtt tattggcgag gatctcaata
     2761 ctcaaatcac cgtgttcatc cgggaacagg gccagttcaa ggtcattaac cggaccggtt
     2821 gccagggtat gggtttgcgc ctgaacacca ggaatatcca gttggtaatc aaatacctta
     2881 atattgagta ccggaccaaa cagcggttca tcacctgccg ctcgcccgct gtcacggaca
     2941 atttgttcgg catcgtaacg ttgatggcga cgcatttttt tcagttgagc cgccagtcgg
     3001 gttgccagtt ccggcagcgt ttcttgcgcc gcaatgtgaa tacccaacgg taaaacgttg
     3061 agcacgggtc cgctagccgt cagcgccgcc gagcccagtc gacgcataaa gataaatccg
     3121 gcggcatagt ccatgcgatt gcacaatcgc cccagccaca aggctgccag cgcaagggct
     3181 aaatcggtac gctgcacacc tgaaagttgc gtagccagct ggcggaattc cccgtcggta
     3241 aattccagtt tcaggcgcag aatatctgcc gaagcgctgc gccccggtaa aggtgccgga
     3301 gaaagtgacg cgggcggcgg cagttgacga cgctgttctg cccagaatgc cgcatcacgc
     3361 tgccaggctt cgctttcgcg gtattgctgg tactcttcca ctacatcagc gaaaggcgta
     3421 aacggcgaag caggcgttgg ttcgccacgc agccatgtgc agtaaatatt ggcgatctgg
     3481 cgagtaatgg ccgggaaact gaagccatcg accagcaaat ggtgataacg ctgataccag
     3541 taccagcggt tatccgcaac atgtatcagc tgatgaaaga ccagtggttt accgctatcg
     3601 acgcgcaaat cttgttgcaa atccgtcagc attaatgcct gcgcagtacc gtgcggatca
     3661 atattggttc gcaggtcgat aatttctggc tgttcgaata tcagcgcatc atcgacccac
     3721 tgccagactt cgccgttatc ttccgtaaaa cgcatccgca acgtatctgc ttgcgctagt
     3781 ccggcaacca ccgcgcgggc cagtaatggc gcatcaacat ctccagttaa ctcaacgtaa
     3841 tgcgccacgc tccaggcgga gggtaaatcc gacagttttt ctgccatcca gatgccgggc
     3901 tgtgcggcga ccaaaggtaa atgctggctc attgtgcctc ctgcaactgg gtgaaattgg
     3961 ccggtgtcag agtacgccag tggacttcca gccactgctg gcaggacgcc tgtgactgcg
     4021 gctgacacac aatgtcccag cctgccgata agaggcattg ttgcggccac agactgaatt
     4081 gcccctgcgc attgcgcaaa atgtaaaacg ctccctgcgg atcatcgaag ggattactga
     4141 atgccatact caactcctgt catggaaaag tggttgccag aggtcgatta acccctgcat
     4201 caagccaccg cgccaacaaa gcgcatcatg tccgccgtca acctgacgcc agaaaatgga
     4261 ttcttttata gggtgcaatt gcgcatacag cgcctgattg gcccgcatga tcatcggctc
     4321 gcgaataccc gcttccagca caatgcgcag accttcggcg ctaacttcac cagcttttag
     4381 cttttcaagt aacacgccct cttgctgccc gtcccgatgc ggccaccagt acgatcctga
     4441 ctggcttaat acacagccaa agcgttcagg ccagtgcagt ccggcataca gcgcggacag
     4501 cccaccaaaa ctctgcccgg cgaccacggt gcgatcggcg cgatcgctaa aaggggcaat
     4561 agctttcacc aggggtaata actcttgctg tactgccagc cagaaatccg cattacacgg
     4621 cagttcgtgg gcgcggtgcg tggtgtcgat agcgtcgatc aacacataca cggcgggagg
     4681 aagttgctga cgatgggtca gcgaagtcag cacaggccag acgggcatac tttgcgccca
     4741 aaattcgcca tcgagcaaaa ctgccagcgg acgttcttct gctgttacat cgccggtggt
     4801 aaaaatccat acacgacgtg aatttttcaa ccgttcactt ttccagataa tttctttggc
     4861 tgctatttct ggcacttgcg gacaatccca tcccggttgc agaggcgctt gcggcatttc
     4921 gagtgcagaa acagcgtgcc ctcgcccgcc tttccagctt tgtggattca gcggatcggc
     4981 tattgcctgg ggtaatagtt ttcgccagcc ttcacgtaat tcgaggcgat cgggggatgg
     5041 tgcagaaaaa atgtcatcgc gttcggtggg aataaagcag tagctgccgc gccagttggc
     5101 attgagttgt gtcgtccact gccagacatc agtacctgca attcgctgca tcgactgggg
     5161 ctggctgttc tgatgatgat cggtcacacc agtgatgtag atccatacgc gctttatcgt
     5221 cgagtattct tcagaacctt ggggatcacg ccaccagaaa gtgacctcaa acatttcgtc
     5281 attcagacgc tgccattccg ggccatgttt cgactgccac cagctctcac ttcctacttt
     5341 taacgccgtc acaccataac cccatgttta ctgtgcaatt tttcattgat tgcagaaata
     5401 tattgataat attattgata accatttgca tttgcaatag cgtaatggcg cgccgtggga
     5461 agcgcggaca tttattaacc aactgcactg cgtgtctttc aggatcaaag gttttcgcgg
     5521 tagcgggatg cgtcgtgttg aggacgacca tgcccgacag ttgcaattcg tggcaaaaat
     5581 gcaggaataa aacaatgaac aagaagattc attccctggc cttgttggtc aatctgggga
     5641 tttatggggt agcgcaggca caagagccga ccgatactcc tgtttcacat gacgatacca
     5701 ttgtcgttac cgccgccgag cagaacttac aggcgcctgg cgtttcgacc atcaccgcag
     5761 atgaaatccg caaaaacccc gttgcccgcg atgtgtcgga gatcatccgt accatgccag
     5821 gcgttaacct gaccggtaac tccaccagtg gtcagcgtgg gaataaccga cagattgata
     5881 ttcgcggtat gggtccggaa aacacgctga ttttgattga cggcaagccg gtaagcagcc
     5941 gtaactcggt gcgtcagggc tggcgtggcg agcgcgatac ccgtggtgat acctcctggg
     6001 tgccacctga aatgattgaa cgtattgaag ttctgcgtgg tccggcagct gcgcgttatg
     6061 gcaacggcgc ggcgggcggc gtggttaaca tcattaccaa aaaaggcagc ggtgagtggc
     6121 acggctcctg ggacgcttat ttcaatgcac cagaacataa agaggaaggt gccactaaac
     6181 gcaccaactt cagcctgacc ggtccgctgg gcgacgaatt cagcttccgc ttgtatggca
     6241 acctcgacaa aacccaggct gacgcgtggg atatcaacca gggccatcag tccgcgcgtg
     6301 ccggaacgta tgccacgacg ttaccagccg ggcgcgaagg ggtgatcaac aaagatatta
     6361 atggcgtggt gcgctgggat ttcgcgccat tgcaatcgct ggaactggaa gcaggttaca
     6421 gccgccaggg caacctgtat gcgggcgaca cccagaacac caactccgat tcctataccc
     6481 gctcgaaata tggcgatgaa accaaccgtc tgtatcgcca gaactactcg ctgacctgga
     6541 acggcggctg ggataacgga gtgaccacca gcaactgggt gcagtatgaa cacacccgta
     6601 actcgcgtat tccggaaggt ctggcaggcg gtaccgaagg gaaatttaac gaaaaagcga
     6661 cacaggattt cgtcgatatc gatcttgatg acgtgatgct gcacagcgaa gttaacctgc
     6721 cgattgattt cctcgttaac caaacgctga cgctgggtac ggagtggaat cagcaacgga
     6781 tgaaggactt gagttccaat acccaggcgc tgaccggggc gaacaccggc ggcgctattg
     6841 atggtgtgag tgccaccgac cgtagcccgt attcaaaagc agaaattttc tcgctgtttg
     6901 ccgaaaacaa catggagctg actgacagca ccatcgtaac gccggggctg cgtttcgatc
     6961 atcacagtat tgtcggcaat aactggagcc cggcgctgaa catatcgcaa ggtttaggcg
     7021 atgacttcac gctcaaaatg ggcattgccc gtgcttataa agcaccgagc ctgtaccaga
     7081 ctaacccgaa ctacattctc tacagtaaag gtcagggctg ctatgccagc gcgggcggct
     7141 gctatcagca aggtaacgat gacctgaaag cagaaaccag catcaacaaa gagattggtc
     7201 tggagttcaa acgcgacggc tggctggcgg gcgtcacctg gttccgtaac gattatcgca
     7261 ataagattga agcgggctat gtggctgtag ggcaaaacgc agtcggcacc gatctctatc
     7321 agtgggataa cgtgccgaaa gcagtggttg aaggtctgga aggatcgtta aacgtaccag
     7381 ttagcgaaat ggtgatgtgg accaataaca tcacttatat gctgaagagt gaaaacaaaa
     7441 ccacgggcga ccgtttgtcg atcatcccgg agtacacgtt gaactcaacg ctgagctggc
     7501 aggcgcggga agatttgtcg atgcaaacga ccttcacctg gtacggtaag cagcagccga
     7561 aaaaatacaa ctacaaaggt cagccagcgg ttggcccgga aaccaaagaa atcagtcctt
     7621 acagcattgt gggtctgagc gcgacctggg atgtgacgaa gaatgtcagt ctgaccggcg
     7681 gcgtggacaa cctgttcgac aaacgtttgt ggcgtgcggg taatgcccag accacgggcg
     7741 atctggcagg ggccaactat atcgcgggtg ccggggcgta tacctataac gagccgggac
     7801 gtacgtggta catgagcgta aacactcact tctgatgcta acgtcagatt gttgacaaag
     7861 tgcgcgtcgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa aaatcttgcc
     7921 aattcaatat attgcaggac catgtaggcc tgataagcgt agcgcatcag gcagttttgc
     7981 gtttgtcatc agtctcgaat atggtcgata tgaaaactac gcatacctcc ctcccctttg
     8041 ccggacatac gctgcatttt gttgagttcg atccggcgaa tttttgtgag caggatttac
     8101 tctggctgcc gcactacgca caattgcaac acgctggacg taaacgtaaa acagagcatt
     8161 tagccggacg gatcgctgct gtttatgcct tacgggaata tggctataaa tgtgtgcccg
     8221 caatcggcga gctacgccaa cctgtctggc ctgcggaggt atacggcagt attagccact
     8281 gtgggactac ggcattagcc gtggtatctc gtcaaccgat tggcattgat atagaagaaa
     8341 ttttttctgt acaaaccgca agagaattga cagacaatat tattacacca gcagaacacg
     8401 agcgactcgc ggactgtggt ttagcctttt ctctggcgct gacactggca ttttccgcca
     8461 aagagagcgc atttaaggca tcggagagac aaactgaagc aggttttctg gattatcaga
     8521 taattagctg gaataaacag caggtcatca ttcatcgtga gaatgagatg tttgctgtgc
     8581 actggcagat aaaagaaaaa atagtcataa ctctgtgcca acacgattaa ttgacaacat
     8641 ctggtacgat tcgcccgcag ccatcactga ctgcgggcga aagtgtaaag caggtgcctt
     8701 accactctga cctgacaacc ggatatgcag ggattgctcc ccgcacaacg gctacttctt
     8761 cggttcgtaa gcgagaacag ccttaaactc aatattacgt tcctttacgg tgaactcaca
     8821 cagcgagtct ccgaccagaa gcgtaaatcc cagcaccgtc aaacacagca ctatgactgc
     8881 cacaagggca tatttcgtca gcatgtttat attgcctcca tgcgaagaag ggactaccat
     8941 ccgagttgtt gaggttcgga gtggcagccc caggattgat agaaatatct cctggggctt
     9001 catctttcca gacctcagag taaacctgaa accggaaagc ttcaggcacc cgccgttatc
     9061 ttacccttcc tcagcaaaat atctgtttat ttatacagta agtcctgttt cggacttctg
     9121 ttggcaacac tcgttattgc tcgtaaaacg aaatcgcccg cagccatcac tgaccgcggg
     9181 cgaaagtgta aagcaggtgc cctaccatcc tgacctgaca accggatatg cggggattac
     9241 tccccgcaca acggctactt cttcggttcg taagcgagaa cagccttaaa ctcaatatta
     9301 cgttccttca ccgtaaactc acacagcgag tctccgacca gaagcgtaaa tcccagcacc
     9361 gtcaaacaca gcactatgac tgccgcaagg gcatatttcg tcagcatgtt tatattgcct
     9421 ccatgcgaag aagggactac catccaaatt gttcgggttt ggagtggcag ccccagggtt
     9481 gatagaaata tctcctgggg cttcatcttt ccagacctca gagtaaacct gaaaccggaa
     9541 agcttcaggc acccgccgtt atcttaccct tcctcaacaa aatggctgtt tatttataca
     9601 gtaaatcctg tttcggactt ctcactcctc atttaatcaa ctgaagctga gcccgttttt
     9661 tctgcatgcc tcaatcgtca aaattcttcg ccatcagcca gctctgccat aacttgccac
     9721 attgaaaaag tagccaaatt gcttcagtca ccatcatcaa aaaaagaaac attccagaac
     9781 ttccgcatct cgcccagccg ctccgacttc tatactgaat agaaaacgcc aacataagag
     9841 aaacctatgc cattacccga ttttcatgtt tctgaacctt ttaccctcgg tattgaactg
     9901 gaaatgcagg tggttaatcc gccgggctat gacttaagcc aggactcttc aatgctgatt
     9961 gacgcggtta aaaataagat cacggccgga gaggtaaagc acgatatcac cgaaagtatg
    10021 ctggagctgg cgacggatgt ttgccgtgat atcaaccagg ctgccgggca attttcagcg
    10081 atgcagaaag tcgtattgca ggcagccgca gaccatcatc tggaaatttg cggcggtggc
    10141 acacacccgt ttcagaaatg gcagcgtcag gaggtatgcg ataacgagcg ctatcaacga
    10201 acgctggaaa actttggcta tctcatccag caggcgaccg tttttggtca gcatgtccat
    10261 gttggctgcg ccagtggcga tgacgccatt tatttgctgc acggcttgtc acggtttgtg
    10321 ccgcacttta tcgccctttc cgccgcgtcg ccatatatgc aaggaacgga tacgcgtttt
    10381 gcctcctcac gaccgaatat tttttccgcc tttcccgata atggcccgat gccgtgggtc
    10441 agtaactggc aacaatttga agccctgttt cgctgtttga gttacaccac gatgatcgac
    10501 agcattaaag atctgcactg ggatattcgc cccagtcctc attttggcac ggtggaggtt
    10561 cgggtgatgg ataccccgtt aacccttagc cacgcggtaa atatggcggg attaattcag
    10621 gctaccgccc actggttact gacagaacgc ccgttcaaac atcaggagaa agattacctg
    10681 ctgtataaat tcaaccgttt ccaggcctgt cgctatgggc tggaaggcgt cattaccgat
    10741 ccgcacactg gcgatcgtcg accgctaacg gaagacacct tgcgattgct ggaaaaaatc
    10801 gccccttccg cacataaaat tggtgcatcg agcgcgattg aggccctgca tcgccaggtc
    10861 gtcagcggtc tgaatgaagc gcagctgatg cgcgatttcg tcgccgatgg tggctcgctg
    10921 attgggctgg tgaaaaagca ttgtgagatc tgggccgatg actaagtagg gaattgcctt
    10981 acagaagaat atccctgaca atggtgtttt aacttacatt taacaatgtt atgaaacacc
    11041 ctttagaaac cttgactacc gcagcaggca ttttgctgat ggctttcctc tcttgcctgc
    11101 tgctgcccgc ccccgcactg gggctgacgc tggcacaaaa actggtgacc atgttccatc
    11161 tgatggatct tagctagctt tacactttat tgttttgtct gtggttttta gtgctgggcg
    11221 ctattgagta ttttgttctg cgctttatct ggcgacgctg gttctcgctg gcggattaaa
    11281 cgtcgattat cgccgtactt cgcggcatac tttgcttatt ctccttcgcc taaaggaacg
    11341 tttatggata agcaatcact gcacgaaacg gcgaaacgcc tggcccttga gttacccttt
    11401 gtcgagcttt gctggccttt tggcccggag ttcgatgttt ttaaaattgg cggcaaaatt
    11461 tttatgctgt cgtcggagct gcgcggcgtc ccctttatca atctgaagtc cgatccacaa
    11521 aaatccctgt taaatcagca gatatacccg agcattaagc cagggtatca catgaataaa
    11581 aagcactgga tttcggtgta tcccggcgag gaaatctccg aagcgttact tcgcgatctg
    11641 atcaacgatt cgtggaatct ggtggttgat ggtctggcta aacgcgatca aaaaagagtg
    11701 cgtccaggct aaagcggaaa tctatagcgc atttttctcg cttaccattt ctcgttgaac
    11761 cttgtaatct gctggcacgc aaaattactt tcacatggag tctttatgga tatcatttct
    11821 gtcgccttaa agcgtcattc cactaaggca tttgatgcca gtaaaaaact taccccggaa
    11881 caggccgagc agatcaaaac tctcctgcaa tacagcccat ccagcaccaa ctcccagccg
    11941 tggcatttta ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa atccgctgcc
    12001 ggtaattatg tgttcaacga acgtaaaata cttgatgcct cgcacgtcgt ggtgttctgt
    12061 gcaaaaaccg cgatggacga tgcctggctg aagctggttg ttgaccagga agatgctgat
    12121 ggccgctttg ccacgccgga agcgaaagcc gcgaacgata aaggtcgcaa gttcttcgct
    12181 gatatgcacc gtaaagatct gcatgatgat gcagagtgga tggcaaaaca ggtttatctc
    12241 aacgtcggta acttcctgct cggcgtggcg gctctgggtc tggacgcggt acccatcgaa
    12301 ggttttgacg ccgccatcct cgatgcagaa tttggtctga aagagaaagg ctacaccagt
    12361 ctggtggttg ttccggtagg tcatcacagc gttgaagatt ttaacgctac gctgccgaaa
    12421 tctcgtctgc cgcaaaacat caccttaacc gaagtgtaat tctctcttgc cgggcatctg
    12481 cccggctatt tcccctcaga ttctcctgat ttgcataacc ctgtttcagc cgtcatcata
    12541 ggctgctgtt gtataaagga gacgttatgc aggatttaat atcccaggtt gaagatttag
    12601 cgggtattga gatcgatcac accacctcga tggtgatgat tttcggtatt atttttctga
    12661 ccgccgtcgt ggtgcatatt attttgcatt gggtggtact gcggaccttc gaaaaacgtg
    12721 ccatcgccag ttcacggctt tggttgcaaa tcataaccca gaataaactc ttccaccgtt
    12781 tagcttttac cctgcagggg attatcgtca atattcaggc ggtattctgg ctgcaaaaag
    12841 gcaccgaagc ggcagatatt ctgactacct gcgcgcagtt gtggatcatg atgtatgcgc
    12901 tgctttcagt cttctcgttg ctggatgtta ttttgaatct ggcgcagaaa ttcccggcgg
    12961 catctcagtt accgctgaaa gggatatttc aggggattaa actgatcggc gcgatcctgg
    13021 tcggcatttt gatgatctcg ctgctgattg gtcagtcgcc agcgattctg atcagcggtc
    13081 ttggtgcaat ggctgccgtg ctgatgttgg tatttaaaga tccgattctt ggtctggtgg
    13141 ccggtattca gctttccgcg aacgatatgc tgaaactggg cgactggctg gagatgccga
    13201 aatacggcgc agatggcgcg gtgatcgata ttgggttaac caccgtcaaa gtgcgtaact
    13261 gggacaatac cattaccact attcccacct ggtctctggt ttctgactcc tttaaaaact
    13321 ggagcgggat gtcagcatct ggcgggcgac gtattaagcg cagtatcagt attgatgtca
    13381 ccagtattcg ttttcttgat gaagacgaaa tgcaacgtct gaataaagcg catttgttaa
    13441 agccttattt aaccagccgc catcaggaaa ttaatgagtg gaatcgccag caaggttcta
    13501 cggagtcggt attaaacctg cgccgaatga ccaatattgg aacctttcgt gcctatctga
    13561 acgaatatct gcgtaaccat ccgcggattc gtaaagatat gaccttaatg gtacgccaac
    13621 tggctccagg tgataacggt ttaccgctcg agatctacgc gtttactaac accgtggtgt
    13681 ggctggaata tgaaagcatt caggctgata tattcgatca catatttgcg attgtcgaag
    13741 agtttggtct gcgacttcat cagtcgccaa ccggcaatga tattcgctct ctggcgggtg
    13801 catttaagca gtaattaaaa aaaccgctct catcgaatgg atgagagcgg acatcgtttt
    13861 gtcggatgta gcgtgaatgc cttatttccg acgcagcgtt ttaaatgcca caaacaggaa
    13921 tacaatccac accggcagca ggatcgctga caagcgcata tcatccatcg tgcacatcag
    13981 cagcaaaatc atgccgagga aggcaatgca gagatagttt ccgaacggat agagcagcgc
    14041 cttaaactgt gtttcacgcc cctgacgtcg catcgctgca cgaaaacgca gatgcgccag
    14101 acagatcata atccagttca acagcagcgt tgctaccacc agcgccatca gcagaccaaa
    14161 cgctttttgc ggcagcagat agttgattaa caccaccagc gaagtgatcg ctccggaaag
    14221 catcagcgag ttaatcggca caccgcgacg gctgacgcga gtcaaaaact tcggcgcatt
    14281 accctgcaca gaaaggccaa acagcatgcg gctgttagag taaaccccgc tgttatacac
    14341 tgacagcgat gctaccagaa tgacgaagtt cagcgcagaa gctaccacgt tgctgtcgag
    14401 attatggaaa atcatcacaa acgggctact gttggatttc acttccaccc acggatagag
    14461 cgccagtaaa accaccagtg aaccgatgta aaacagcagg atgcgataca ccacctgatt
    14521 taccgctttt ggaatgcttt tatccggatc gcgcgcttca gcggcagtaa tcccaatcag
    14581 ctccagaccg ccgaaggaga acataattac cgccagcgac aaaatcagcc cattccagcc
    14641 ggtggcgaag aaaccaccgt agcgccagag gttgtcgata ctggctttct cgccgccgtg
    14701 accagaaaac agcagccaca ggccaaagcc gatcataccg atgattgcca gcactttaat
    14761 caacgcaaac cagaactcgg tttcgccata taagcgcacg ttcaccaggt taacggcgtt
    14821 gatgataata aagaaggcgg cagcccaaat ccacgttgga acatccggga accagtactg
    14881 catatagatg cccgcagcgg tcagctctgc cattcccacc agcacgaaca ttacccagta
    14941 gttccagcca gagaggaagc ccgcaaacgg tccccagtat ttataggcaa agtgggcaaa
    15001 tgaaccggat accggctctt cgaccaccat ctcgccaagc tggcgcataa tcaggaaagc
    15061 gatgatcccg gcgacgccgt agcccagcaa tacagccgga cccgccatct gaatcgccgg
    15121 accaatgcca agaaacagac cagtaccaat tgcgccaccc agcgcaatca gttgaatatg
    15181 acggttatgt aatccgcgat gaagcgtcgg ctcttgattc gacgcagtat cttccgatac
    15241 ggttgacgcg tttttcacgc ctttcccctg tgtgtctttt ttgttgaggg gcacctttta
    15301 acatttagtg cccatcgtcg caagacacaa tccacacggt taaacggggt atcctgcttt
    15361 tatttccgta cccgatgtcg gtgcagccac atcagcttat acgccgccgg gataataaac
    15421 agcgacagca aaggtgcggt gatcatgccg ccaatcatcg gtgcggcaat ccggctcatc
    15481 acctctgaac cagcccccgt tccccacaga atcggcagca gaccggcgat aatcaccgcc
    15541 accgtcatcg ctttcgggcg cacgcgcagt accgcgccgt gatacaacgc ctcatccagc
    15601 ttctgctcac tgaatgtttg cggattattc aacgacggct cggcctctat ggcgtgacgt
    15661 aaatacatca gcatcaccac gccaaattcg gcggcgaccc cggcaagggc aataaaaccg
    15721 gtgcccgttg ccacagaaag atgaaagccc atccaccaca gcaaccagat gccgcccacc
    15781 agcgcaaacg gtacgctgct gataatcagc aacgcttcgc ccacccgacg gaatgccaga
    15841 tacaacagca cgaagatgat catcagtgtc atcggcacca tgagtttcag cttatggttg
    15901 gcgcgctcca gtagctcgaa ctgcccggag aatgccacgc tggtgcccgg ttttaactgc
    15961 actttttcag ctatcgcttt ttgcaaatcg tgaaccaccg acaccatgtc acgatcgcgg
    16021 gcatcgatat aaatccagct cgtcgggcgc gcattctcgg ttttcagcat cgacggtccg
    16081 gtagagactt taacgtcggc cacgtctgcc agggtgattt gctgcttcat cggcgtcagg
    16141 atcggtagct ggcgcagtgc ctgcgggcta tcgcgccagc tttgcggata acgcagatta
    16201 atgggataac gggcaatccc ttccaccgtt tcgccaacca tcgccccgcc caccgcagaa
    16261 gtcacaaaca actgcacatc cgccaccgtc ataccgtaac gcgcggcttt ttcacggtta
    16321 atctcaacgt tgatatagcg cccaccttcc agacgctcgg ctagagcaga agccacgctt
    16381 ggcaccgttc gcgctacttc ttcaatttgc tcagccatcg cgtcgatatc cgccagcaca
    16441 gtgccggaaa ctttaatgcc gatggggctt ttaatgccgg ttgagagcat atcgatacgg
    16501 ttacgaattg gcggcaccca cagattcgcc agccccggca gccgcacggt gttatccagt
    16561 tcctcaatga ttttgtccat cgtcatgcca ggccgccact gatcctgcgg cttaagctgg
    16621 atggtcgttt caaccatctc gagcggagca gaatcggtgg cggtttccgc tttcccggtt
    16681 ttgccaaata cccgcgccac ttcaggtacg ctcataatta gcttgtcggt tttttgcagc
    16741 atactcgccg cctctgctgc ggaaatcccc ggcagcgtcg atggcatata caacaagtcg
    16801 ccttcattga tctgcggtaa aaattccccg ccaactttat tgagcggcca gagaaccgtc
    16861 agcaccgaaa gcgctgccac caacagcgtg gttttcggcc agtgcagtac tttcagcaat
    16921 agaggatgat aaacacgaat caaaaagcga ttgagcgggt tactgctttc cggcggaatt
    16981 ttgccacgga tccagtaccc catcaggata gggatcacta cgatcgccag cagcgccgcg
    17041 cccgccatcg cataggtttt agtgaacgcc aacgggccaa acagacgccc ttcctgccct
    17101 tccagggtga agatcgggat aaacgacaac gtgataatca gcagactgat aaatagcgcc
    17161 ggacccactt caacagatgc atcggtgatc acctgccagc gcgttttatt atccagcgtg
    17221 gcgtcaggat gctggtgctg ccactcttcc agccgtttat gtgcattttc gatcatgacg
    17281 atagcggcat cgaccatcgc cccgacggca atcgcaatgc cgcccaacga cataatattg
    17341 gcattcagtc cctggaagtg catgacaata aaagcaatac acaaccccag cggcaacgaa
    17401 ataatcgcca ccagcgccga gcgcacatgc cagagaaaca gcgcacagac taccgccacc
    17461 acgataaact cttccagcaa cttgccgctg aggttgtcga tggcgcggtc aatgagctgg
    17521 ctgcgatcgt atgttgtaac tatctccacg ccttccggca gactactttt cagcgtttcc
    17581 agtttgtcct tcacggaggc gatcacttct cgggcgtttt tgccggagcg caggatcacc
    17641 accccgcccg ccacttcgcc ttcgccattc agttcggcaa tgccccggcg catctccggg
    17701 ccaatctgga ccttcgcaac atcacgcaga taaacgggca cgccattttc actggctttt
    17761 aaaacgatgt gattaaagtc gtcgagcgtt tgcagatagc cgctggcgcg caccatatat
    17821 tccgcttccg ccagttcgat cgacgaaccg cccgcttcct ggtttgaagc atccagcgcg
    17881 ctttttactt cggctagact gatgccatat tgcgccaggc gctggggatc gataaccacc
    17941 tgatactctt tcaccacgcc gcccaccgac gccacttccg caacgtcagg gatggttttt
    18001 agctcatatt tgagaaacca gtcctgcaat gagcgtaaat cggccagatc gtgcttaccg
    18061 ctgcgatcca ccagtgcata ttcatagatc cagccaacac ccgtggcatc tggtcccagc
    18121 tcggcgctga ctcccgcagg cagcttaccc tgtacctggt tgaggtactc cagcacccgc
    18181 gaacgcgccc agtacggatc ggtaccatct tcgaaaatga catacacata agagtctcca
    18241 aactgcgaga aaccgcgcac agtcttcgcg ccaggcaccg acaacatggt ggtggttagc
    18301 ggataagtca cctgattttc aacgatttgc ggtgcctgac cgggatagct ggttttaata
    18361 atcacctgca catcggagag atccggcagc gcatccactg gcgtgttaat gatggtccag
    18421 gtgccccaga tgctcagaaa caacgcgccc atcagcacca aaaaacggtt cgccaccgag
    18481 cgacgaataa tccattcaat cattggttat tccctcaatg cgcattggta gcactttcag
    18541 agcgcatccg ctccagtgcg ccagaaatat tggcttcgga atcaatcagg aacaggccgc
    18601 tggaaaccac cttttcacct tccgccagac cagagtgtaa cgcggtgacg ccttgcgatg
    18661 cctggaaaac ggcaacgcgt ttcggtacaa agcgtccgtc ggcatcaacg gtaatcaccc
    18721 gctgttcgtt gccggtatca atcagcgcct gtgacggaat gagcagcatc ggttcgctgg
    18781 cggtgttgag ttgcaaccag gcgttcatgc ccggttttag cgcctcgtcg gcgttgtcga
    18841 cttccagacg cagctgcagc gtgcgggtcg cggcatccac gccaggtagc agcgtccatt
    18901 tgcggatggt gagtgtttta tccggtcgcg ccggaacggt gagcgtaaac tgcgaggcat
    18961 ctttcaccag ccaggcgata gactccggga tcgcagcagt gacccacacc gggtccatac
    19021 cctgaatttt cgctaccacg ttatctttgg cgatattcat tcccgcgcgc agatcaaacg
    19081 cggtgatcac gccatcaatg ggtgctttga gcgtaaagcg agtctggatt ttttgcgtgg
    19141 cgatcaggcg gcgaatatcc gcctccggca ttcccgccag tcgcagccgc tcaagaatgc
    19201 cttcagtctg ggtcgccgta ccgccggttt cgcgcagcag taaatactca ctctgcgctt
    19261 ccacccagtc aggaatggtc aggtcgagaa gcggtgtgcc cttttgtact ttatcgccca
    19321 cggtaagcgg atacaccttg tcgataaacc cggcagcgcg agcctgcaca atggcatact
    19381 gatactcgtt gtaactgaca ttcgccggga aactctgggc aaaagtcagc ggtccgcgcg
    19441 tgacggtagc cgttttcacc cccagattct gcgtctgagt cgggtcaatg cgcacaccag
    19501 acgcagaact ctcttcatcg gcatatttcg gcaccagatc catatccata aacggcgatt
    19561 tacctggttt atcgaaccgc gtattggggt acatcgggtc gtaccagaat aagattttac
    19621 gttctgcggt cgacgttttt tctgcgggtg gttcagcctt tgcaaaccag gtaaaacctg
    19681 ccgcagaaat aataccgccc gcgatcatgc tgccgataat aagcgcgatt tttttcatct
    19741 cattaaacct gggttactgg ctgactttaa tatcctgtaa taaagaaagg ttgccctgct
    19801 ggacaaaatt aaacgccact ttgtcgccgg ttttaatttc actcattttc gtctgcgggg
    19861 tgatggtaaa gcgcatagtc atctccggcc agttcacggc agcaatcgga tcgtgatgga
    19921 tggtgatttt tttgctttcc agatcaatac cctttaccac gccagtggcg ctaataacct
    19981 gcggttgtac ttcgctcatg gtttcatgat gatgttcgtt agcctgggca ttaaagccaa
    20041 taacggtaaa cagactgaac attgcgactt gcagtgcttt tttcatttat tctctcctgg
    20101 agttaaaaat tattgctgcc aaccgccccc taaggcggta tacaaagaaa tttcgttaac
    20161 ctgacgggca taattcaggt cgagtaaagt ttgtcgggtt gcaaataaag aacgctcggc
    20221 atccagcact tccagataac ttaccgcgcc gtgctgatat aatgcccgcg cccgttgcaa
    20281 agtaatttgc agcgacgcca gataacgctg ctgggcgctg atttgatcgt tcaggctttg
    20341 acgtaatgcc agcgcatctg ccacttcttt aaaggcgttc tggattttct gttcataatt
    20401 cactaccgac tgctgctggc gaatttcggc gatatccaga ttggcctggt tgcgtccggc
    20461 attaaaaatg gggatctcaa ttttaggaat aaaattccac atcccgctgc tagcgttaaa
    20521 taatgaagac agatcgctgc tggcggttga tattccgctg gtcaggctga tagacgggaa
    20581 aaatgccgca cgcgcagcac caatattagc attagccgcc attaacgcgt gttcagcttc
    20641 cataatatcc gggcgctgca ataagatttg cgacgacaag cccgccggta atttaacgct
    20701 ttgcaggctg ccgctgttta ctgtctgcgc ttgcggcagt ttgccgtagc ttcccaataa
    20761 cagttgcaat gcattattcg cctgcgccag ttccccctga cgtttagcga tgtcactgcg
    20821 ggtactttct atcaccccgc gagcctgttc cagcgccaga acattgctgc taccggtcaa
    20881 cagttgtttt tcgacaaacg catatgactg ctgataatta cgcagcgttt cttcggctat
    20941 ttgcaattgc gcatacgcca gttgctgatt gaaatagctt tgcgcgacat tagaaaccag
    21001 cagaatatgc accgcgcgct gagcttcctc agtggctaaa taattttgtc gctcggcttc
    21061 gctcatgttc tttaagcgac cgaaaaaatc gaggtcaaag ctggcgttaa ggccggtcga
    21121 aaactcccgt gtcgtggcgg tattcccttt aagattaccg ctccagctgc cgctgccctc
    21181 gccattgagc tgtgggtagc ggtcggcatc ggtcagacga tattgcgccc gtgcctcctg
    21241 cactttcagc gtcgccatgc gcaaatcccg gttattcacc agcgcctcgc taatcagcgt
    21301 cttcacctga ttatcaacaa aaaaggtgcg ccagcccacg ttctggtagt tatctgctgc
    21361 gttaaccaag ccgttctggc tgagtgagaa ctgctgcggc acgggcattg ccgtacgctg
    21421 ataatccggt gccaatgaac aaccggttag cgcaagggcc acacaaaatg gcagaagttt
    21481 acaaggagac ataggctcat aatttctggt gattttatac cgccaacttt actcgccagg
    21541 ctctgatttt ccggtgacag gaaaatgaca aaattgtcat tttgccaata agcgattgcc
    21601 atctgatccc gctactctag aattgcccgg gcaacatgcg gaggaaaaat gaaactgttg
    21661 attgtcgaag atgaaaagaa aaccggagaa tacttgacca aagggttaac cgaagccggt
    21721 tttgtggtcg atttggccga caacgggctg aatggctacc atctggcgat gaccggtgat
    21781 tatgacctga taatcctcga tattatgctg ccggacgtga acggctggga tatcgtgcgc
    21841 atgttgcgct ccgccaataa agggatgccg attctgttgc ttaccgcgct tggcaccatt
    21901 gaacatcgcg tcaaggggct ggagttgggg gccgatgact atctggtgaa gccgttcgct
    21961 tttgctgaac tgctggcgcg ggtgcgcact ctcctgcggc gcggggcggc ggtgattatc
    22021 gaaagtcagt ttcaggttgc cgatctgatg gtcgatctcg tcagccgcaa agtcacccgc
    22081 agcggcacgc gcatcactct gaccagtaaa gagtttactc tgctggagtt ttttctccgc
    22141 catcagggcg aagtgctgcc ccgctcgctt atcgcctcgc aggtgtggga catgaatttc
    22201 gacagcgaca ctaacgccat tgatgtggcg gtgaagcggc tacgcggcaa aatcgacaac
    22261 gactttgagc cgaagctgat tcagaccgtg cgcggcgtgg gctatatgct tgaggtgccg
    22321 gatggtcagt aagccatttc agcgcccgtt ttcgctggca acccgtctga ccttttttat
    22381 cagcctggcc accatcgcgg cgtttttcgc ctttgcatgg atcatgatcc actcagtgaa
    22441 agtgcatttt gccgagcagg atattaatga tttaaaagaa attagcgcca cccttgaacg
    22501 ggtacttaat catcctgacg agacgcaagc ccgacgctta atgacgctgg aagatatcgt
    22561 cagtggttat tccaacgtgt tgatttccct ggcagatagc cacggcaaaa cggtgtatca
    22621 ctcccccggt gcgccggata tccgcgagtt tacgcgtgac gccatacccg ataaagacgc
    22681 tcagggtggc gaggtgtatc tcctttccgg cccgacgatt atgatgccag gccacggtca
    22741 cgggcatatg gaacacagca actggcggat gattaacttg ccggttggcc cgttggtgga
    22801 cggcaaaccg atttatacgc tctacatcgc gctttcgatc gattttcatc ttcattacat
    22861 aaatgatttg atgaataaac ttattatgac cgcatctgtc atcagcatcc tgatcgtctt
    22921 tatcgtgcta ttggcggtac ataaaggtca cgcgccgatc cgcagcgtca gccgtcaaat
    22981 ccagaatatc acctcgaaag atctcgacgt tcgccttgac ccgcagaccg agcccattga
    23041 gctggaacag ctggtactgt cgttcaacca tatgatcgag cgtattgagg atgtctttac
    23101 ccgccagtcc aatttctcag cggatatcgc tcacgaaatt cgcacgccga ttaccaatct
    23161 catcacgcaa acggaaatcg ccctcagcca gtctcgcagc cagaaggagc tggaagatgt
    23221 gctctactct aatctcgaag agctgacgcg aatggcgaaa atggtcagcg atatgctgtt
    23281 tctcgctcag gccgataaca accagctaat ccccgaaaag aaaatgctca acctggcgga
    23341 tgaagtcggc aaagtgttcg attttttcga ggcgttagcg gaagatcgcg gcgtggagtt
    23401 gcgatttgtt ggcgacaaat gtcaggttgc gggcgatccg ctgatgctgc gtcgggcgtt
    23461 aagcaacctg ctttctaatg ccctgcgtta tacgccaacc agagagacaa ttgtagtgcg
    23521 ctgtcagacg gtcgatcacc aggtgcaagt taccgtcgaa aaccccggta cgcccattgc
    23581 gcccgagcac ttaccgcgat tgtttgaccg tttctaccgc gttgaccctt cccgccagcg
    23641 aaaaggcgaa ggtagcggca ttgggctggc gatagtgaaa tcgattgttg tcgcgcataa
    23701 aggcacggtt gcagtaacgt cagatgcgcg ggggacaagg tttgtgatta tgctgccgga
    23761 gagagagtga ttattaagtt caaattcagg gcgtggtgca tcacctcctg gtat
//
LOCUS       DAZRUF010000041        23405 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZRUF010000041 DAZRUF010000000
VERSION     DAZRUF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23405
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.041"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHXOL_001315"
     CDS             complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHXOL_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR6808336.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHXOL_001316"
     CDS             complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHXOL_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFR6808337.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHXOL_001317"
     CDS             complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHXOL_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR6808338.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHXOL_001318"
     CDS             complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHXOL_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR6808339.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHXOL_001319"
     CDS             complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHXOL_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR6808340.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHXOL_001320"
     CDS             complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHXOL_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR6808341.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHXOL_001321"
     CDS             complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHXOL_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR6808342.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHXOL_001322"
     CDS             complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHXOL_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR6808343.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHXOL_001323"
                     /pseudo
     CDS             complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHXOL_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHXOL_001324"
     CDS             complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHXOL_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR6808344.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHXOL_001325"
     CDS             complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHXOL_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR6808345.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVVCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHXOL_001326"
     CDS             complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHXOL_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR6808346.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHXOL_001327"
     CDS             complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHXOL_001327"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR6808347.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14171..14743)
                     /locus_tag="ACHXOL_001328"
     CDS             complement(14171..14743)
                     /locus_tag="ACHXOL_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR6808348.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14889..15767
                     /gene="dapA"
                     /locus_tag="ACHXOL_001329"
     CDS             14889..15767
                     /gene="dapA"
                     /locus_tag="ACHXOL_001329"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR6808349.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15784..16818
                     /gene="bamC"
                     /locus_tag="ACHXOL_001330"
     CDS             15784..16818
                     /gene="bamC"
                     /locus_tag="ACHXOL_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR6808350.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17031..17744
                     /gene="purC"
                     /locus_tag="ACHXOL_001331"
     CDS             17031..17744
                     /gene="purC"
                     /locus_tag="ACHXOL_001331"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR6808351.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17912..18760
                     /locus_tag="ACHXOL_001332"
     CDS             17912..18760
                     /locus_tag="ACHXOL_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR6808352.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHXOL_001333"
     CDS             18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHXOL_001333"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR6808353.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRGSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHXOL_001334"
     CDS             20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHXOL_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR6808354.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21672..21872)
                     /locus_tag="ACHXOL_001335"
     CDS             complement(21672..21872)
                     /locus_tag="ACHXOL_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR6808355.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHXOL_001336"
     CDS             complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHXOL_001336"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR6808356.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23031..23387)
                     /locus_tag="ACHXOL_001337"
     CDS             complement(23031..23387)
                     /locus_tag="ACHXOL_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR6808357.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         5853 a         6225 c         6268 g         5059 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc agtgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgctcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcaagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacactg tactgtcgct cttcgccaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgacg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctacgcca ggcatcggca ttgccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctgaca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat tatccgcgcc
     2341 agccagttaa acagtgtgca atcctgggga gtaaaacggc agccgtgggt gcgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg cggcggcagc
     2581 gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gcgctaaccg ctccgcgagc
     2641 attccgttta ccgcttcaat tgttattcct tgcggcgggg caaacatcgc ctcgtctgac
     2701 atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt ctggccatca
     2761 gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg
     2821 cctgccacac agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc aacagggcgg
     2881 tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc ttacgtcggg
     2941 cttgctcctc ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg caggggaagg
     3001 cgacattcag acaatcaatc acgccaacgt gatgaccgat agccagcgag taatacatta
     3061 cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc
     3121 agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc
     3181 tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt
     3241 cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga
     3301 tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgc
     3361 ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccaccgc gtgaatcttc
     3421 tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg
     3481 cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag
     3541 agatcgtgga aaataccgcc gccgacaccg cacgcgccgt aggaaacaca aattttatga
     3601 tcgggggcag actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct
     3661 gtaaataaca aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct
     3721 gcgtcgaata ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg
     3781 caatcgacgc gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc
     3841 ttttgcgttt gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc
     3901 atagctgctc cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt
     3961 gtttgcactc cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat
     4021 tttgttgctg tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc
     4081 gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat
     4141 tggtgacggt cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt
     4201 cacaacgtcc gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct
     4261 ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac
     4321 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
     4381 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
     4441 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
     4501 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
     4561 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca
     4621 gcatgtaacg caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca
     4681 atttttggtt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
     4741 agcccagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg
     4801 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
     4861 ccatcacgcg ggagaaaaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc
     4921 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
     4981 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag
     5041 tgcgttgttc catattcggc gtggcaagca gcatctctac cagctccgac acgtcggcgc
     5101 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
     5161 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg
     5221 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat
     5281 ggcaggagag gccaaggttg agcaaatgac tgtgcagacg ttcgacttcc agcagaatcg
     5341 agcgaatagt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
     5401 tataggccac actgtgggca aaaccgcaaa tcccacacac gcgatccgat aagaaggtca
     5461 cttcgttgta gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca
     5521 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
     5581 catcggaggt gatatgcagc gggccaatag ggatcacccg cgcatcgcta ttgccctcat
     5641 tgataaacgg atacgtttcg gtatcagtcg tcggttcagg gcgcagtcga taatccatcg
     5701 catctttgcg cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct
     5761 gatccggcag gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga
     5821 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
     5881 ccttcaccac gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt
     5941 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt
     6001 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
     6061 gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg
     6121 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
     6181 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
     6241 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
     6301 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
     6361 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgctgccatc
     6421 cgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg
     6481 tgattacgtg ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc
     6541 ggcatccctg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt
     6601 ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg
     6661 gaaccgcaga acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca
     6721 aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg
     6781 ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg
     6841 gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct
     6901 tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg
     6961 gagagcaggg cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc
     7021 atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc
     7081 attaatgttg ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt
     7141 tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca
     7201 aaagcgacac caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc
     7261 tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac
     7321 atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag
     7381 aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc
     7441 atgtagccaa tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac
     7501 aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca
     7561 gcggcagttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt
     7621 aatacagtca ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc
     7681 gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta
     7741 tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct
     7801 tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac
     7861 cagttccggt gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag
     7921 gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag
     7981 cagaaaatga cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt
     8041 cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat
     8101 tgccggaccg aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt
     8161 catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag
     8221 ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg
     8281 gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt
     8341 cagcatcatc agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct
     8401 tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc
     8461 aacaccaggc gcattgaccc tggcagcggt gcggcatcgg cgacggcctc gctcggtttg
     8521 cccggcacga cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc
     8581 atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccgacg
     8641 gcaaacagcg ggaatttact gaagaagccg ttgaacggcg gcacaccggt aatcgccagc
     8701 gctgccacgc agaaacccac gcttggcaac ggcagggtgt gcaatacgcc acgcagacgc
     8761 ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt
     8821 ttagcgaacg cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg
     8881 aagatggaga gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc
     8941 agcaaccgct tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc
     9001 agcgccatga ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg
     9061 gcgcgggcaa aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcactg
     9121 atcggtgttg gcgcttccat tgcatccggt agccacgctt gcatcggtag ctgggccgat
     9181 ttcccccacg cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac
     9241 tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga
     9301 tacaacccca acgagccgat atgagtgata agtaacgctt ttagtgctga aagctgtgct
     9361 ttatcgctct ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac
     9421 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
     9481 agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc
     9541 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
     9601 agcgtactca tgcgatcaat gaccaagcca aacaatgcca catcccccac gttgaccaac
     9661 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
     9721 agcgtggtca gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg
     9781 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
     9841 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
     9901 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
     9961 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    10021 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    10081 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    10141 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    10201 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    10261 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    10321 cctgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    10381 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagccctgaa
    10441 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    10501 cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    10561 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcatcttcc
    10621 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    10681 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    10741 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    10801 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    10861 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    10921 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    10981 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    11041 tagacgaaat agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg
    11101 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    11161 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    11221 taacagcaac agtaatgaag gagtgacccg tgtatgaaac gtgccgggta caagtgctat
    11281 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt
    11341 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    11401 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtgc tgcgcggcgc
    11461 accacagaaa gtaataccgt acattttgac gaagcacact actgccagcc caccagtgac
    11521 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    11581 tagtgagaat agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    11641 ggctgatatc gccaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    11701 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc
    11761 gagaaatagc agccctttga aaagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    11821 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc
    11881 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    11941 ttccgccagt gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    12001 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    12061 gccgaaaata ccgattttga ccattacgcc agacatcaac gctgaagcgt gcgatggtgc
    12121 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    12181 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    12241 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcattat
    12301 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    12361 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    12421 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    12481 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    12541 gagagaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    12601 cattcgcagg gttaaatcgg caaattcaaa aggcgtggca aaacgggcga ctaaagttac
    12661 gcctgcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccta ccagcgatgt
    12721 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    12781 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    12841 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    12901 tgtttttctt tcatctggcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    12961 gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    13021 gcgacggtct ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct
    13081 tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga
    13141 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    13201 atacagaggc tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg
    13261 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    13321 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    13381 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    13441 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga
    13501 tgccattctg attaactcaa tatattcaat aagttatgta ttaactggat tctgaacggt
    13561 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    13621 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag
    13681 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    13741 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    13801 aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg
    13861 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    13921 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    13981 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    14041 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    14101 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    14161 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    14221 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    14281 cgtccgcaga tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat
    14341 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    14401 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    14461 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    14521 gctcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    14581 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    14641 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    14701 ccaatgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    14761 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    14821 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    14881 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    14941 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    15001 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    15061 atgtggtgat gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg
    15121 gcgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    15181 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    15241 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    15301 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    15361 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    15421 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    15481 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    15541 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    15601 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    15661 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    15721 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    15781 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    15841 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    15901 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    15961 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    16021 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    16081 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    16141 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    16201 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    16261 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg
    16321 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    16381 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    16441 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    16501 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    16561 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    16621 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    16681 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    16741 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    16801 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    16861 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    16921 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    16981 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    17041 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    17101 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    17161 atcgcaaagg tatgttgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    17221 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    17281 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct ctggtgaaac
    17341 gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa
    17401 acgatgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    17461 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    17521 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca
    17581 aaggtgaagt ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag
    17641 aaacactgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag
    17701 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    17761 cttgccgtgc agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca
    17821 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    17881 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    17941 caatgtcgaa gacaggcgta acagctccgg cggtccgtca atgggcggtc ccggttttcg
    18001 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    18061 tggtgttgat ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacacg
    18121 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    18181 ggaagacacc tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct
    18241 ggtcatgtat cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca ttatgggacc
    18301 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    18361 agacaaactc ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    18421 tcatcatgtg cagaaattgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    18481 gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    18541 cggtgtctgg gggcataaca tgcagcagca aggcgatctg gaagaggcgc tgaacgcggc
    18601 gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag
    18661 tttcactcat ggtacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg
    18721 cgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc aaggatggct
    18781 gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg
    18841 ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc
    18901 ttacctggcg actggctgtg gatttcgccg cagccagatg ctgaaaacca ctgttctccc
    18961 tcggcgctac aaactttact tgggcgcgag ttccggcatg cggtattcga cgcccgccac
    19021 ggctttgatg ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg aagctggctg
    19081 gttttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc
    19141 tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgcgta
    19201 cttacggcgg ataacgacgc tattctctgg cggcaaaacc agccgttctc gttggcgcat
    19261 tttacccccc gtactgactg gcaccccgcg accggcgcac cacaaccaga acaacagcaa
    19321 ctcttacagc agctactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg
    19381 cgcggtaagt ccgcgctggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc
    19441 accgcgcccg caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc
    19501 tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat
    19561 gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg
    19621 ttgttaacca ctacggtgca gggctacgaa ggtaccggac gtggtttttt gctgaaattt
    19681 tgcgctcgct ttccgcattt acaccgtttt gaactgcaac agccgatccg ctgggcacag
    19741 ggatgcccgc tggaaaaaat ggttagtgag gcactggttt ttaacgatga aaacttcacc
    19801 catacaccac aaggcaatat cgtcatttcc gcatttgaac aaacgttatg gcgaagcgat
    19861 ccagaaacgc cgttaaaggt ttatcagtta ttgtctggtg cgcactaccg gacctcgccg
    19921 ctggatttac gccggatgat ggatgcacca gggcaacatt ttttacaggc ggctggcgaa
    19981 aacgagattg ccggggcgtt gtggctggtg gatgagggcg gattatctca agaactcagt
    20041 caggcggtat gggcaggttt tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg
    20101 gcgcacggca gcaatccact ggcggcgaca ttgcgcggac ggcggggcag ccggattgca
    20161 gtccatccgg cgcgtcagcg cgaaggcgtt gggcaacagc tcattgccag cgctttgcaa
    20221 tataggcctg gcctcgacta tctttcggtg agttttggtt acaccgggga gttatggcgt
    20281 ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc
    20341 ggttgctata cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa
    20401 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg
    20461 cttcctgttg atccactaaa caatgccgtc ctttctgacg acgactggct tgaactggcc
    20521 ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta
    20581 caaaccagtg aactggcatt accggcgctg cgtgggcgtt tacagaaaaa caccagtgac
    20641 gcgcagttat gtaccacact taaactttca ggccgcaaga tgttactggt tcgccagcgg
    20701 gaagaggccg cgcaggcgct gttcgcactt aatgaggttc gcactgagcg tctgcgcgat
    20761 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
    20821 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
    20881 tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg
    20941 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
    21001 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
    21061 ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac
    21121 gtttattgag atggtgcgct actggcagaa acagagcgga gtaggggcta acgccacagc
    21181 gctaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
    21241 ccttgcttca cgcgtcattg cttttaacgg acgctatgcg agcctgccgg aaactgcgtc
    21301 tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca
    21361 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
    21421 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg
    21481 ttataccatt ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcgatga
    21541 tgatgtgatt gaaatgatgt aaaagacaga ttacctggcc tgataagacg cgtgagcgtc
    21601 gcatcaggca tcgttcacca ttgccggatg cggcgttaac gccttatccg gcctacggtt
    21661 gagcactcag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    21721 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttgtgg
    21781 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    21841 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    21901 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    21961 gcgttcacac attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc
    22021 tgcgccccca tgcgagcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    22081 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    22141 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    22201 tcaagcaggg caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg
    22261 aagttaaact gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga
    22321 atattggcaa tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata
    22381 gccactaatt cattaaggaa aggtgctgcg cgatgtactg gattgtcagc cagatgtggg
    22441 taggccacat gcccctgcac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    22501 ccatttttca ccacatcacc aaccacttcg atactcgacg gttcgccgac cagacagtaa
    22561 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca
    22621 ctggcttctt catcagaggt gatcagaaat gccagtcgtc ccgtatggtt gggatgttgt
    22681 gcgacaaaac gttctgctgc taccaccatc gccgccagcg agcctttcat atctgccgca
    22741 ccgcgcccga ataacatgcc atcacgaatg gtgggttcaa acggcggatt gatccaacga
    22801 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc
    22861 ccacgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    22921 atcgcctgca aacgatcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    22981 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    23041 tggaaaaatt gctgataact ggaatcactg aaacccagca gcataggctt accgggcgcg
    23101 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgctgaggcc
    23161 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    23221 ttgagtaacg cctcccaacc taattcgttg ataaaaccgt tcaataattc gctgtccagc
    23281 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga
    23341 gcctttttaa tggtgtcaca atttttgata ccgtaaagtg taaccataat gaatcctttt
    23401 tcgat
//
LOCUS       DAZRUF010000042        23005 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZRUF010000042 DAZRUF010000000
VERSION     DAZRUF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.042"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHXOL_001338"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHXOL_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR6808358.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHXOL_001339"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHXOL_001339"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR6808359.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHXOL_001340"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHXOL_001340"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR6808360.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHXOL_001341"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHXOL_001341"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR6808361.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHXOL_001342"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHXOL_001342"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR6808362.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="ACHXOL_001343"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="ACHXOL_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR6808363.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11312
                     /gene="ftsK"
                     /locus_tag="ACHXOL_001344"
     CDS             7245..11312
                     /gene="ftsK"
                     /locus_tag="ACHXOL_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR6808364.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11467..12078
                     /gene="lolA"
                     /locus_tag="ACHXOL_001345"
     CDS             11467..12078
                     /gene="lolA"
                     /locus_tag="ACHXOL_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR6808365.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12089..13432
                     /gene="rarA"
                     /locus_tag="ACHXOL_001346"
                     /gene_synonym="mgsA"
     CDS             12089..13432
                     /gene="rarA"
                     /locus_tag="ACHXOL_001346"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR6808366.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13523..14815
                     /gene="serS"
                     /locus_tag="ACHXOL_001347"
     CDS             13523..14815
                     /gene="serS"
                     /locus_tag="ACHXOL_001347"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR6808367.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15054..17498
                     /gene="dmsA"
                     /locus_tag="ACHXOL_001348"
     CDS             15054..17498
                     /gene="dmsA"
                     /locus_tag="ACHXOL_001348"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR6808368.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17509..18126
                     /gene="dmsB"
                     /locus_tag="ACHXOL_001349"
     CDS             17509..18126
                     /gene="dmsB"
                     /locus_tag="ACHXOL_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR6808369.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18128..18991
                     /gene="dmsC"
                     /locus_tag="ACHXOL_001350"
     CDS             18128..18991
                     /gene="dmsC"
                     /locus_tag="ACHXOL_001350"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR6808370.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19027..19653)
                     /gene="ycaC"
                     /locus_tag="ACHXOL_001351"
     CDS             complement(19027..19653)
                     /gene="ycaC"
                     /locus_tag="ACHXOL_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR6808371.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            19968..21116
                     /locus_tag="ACHXOL_001352"
     CDS             19968..21116
                     /locus_tag="ACHXOL_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6808372.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            21326..22756
                     /locus_tag="ACHXOL_001353"
     CDS             21326..22756
                     /locus_tag="ACHXOL_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR6808373.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(22757..>23005)
                     /locus_tag="ACHXOL_001354"
     CDS             complement(22757..>23005)
                     /locus_tag="ACHXOL_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR6808374.1"
                     /translation="DDVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITER
                     PGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         5651 a         5727 c         6085 g         5542 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cataaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc atttttgtgg tgaaaacaag catcgtggca
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataattc agataatccc
      841 gacggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgactg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcatgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca accttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattcaggc caaagcaccg
     1261 cgcgaggatc gggcgaccac cagaggatgg gggcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gccccccaga gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgctta aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcgtcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccaa gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttc cggtttttga tccgttaaat cagtgatccg
     2281 aacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgac
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcgattgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgcg gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatctgacca tcctgcatta cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcatgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgtaat gttgctgccg gtaattgtgg attttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cgcgtaattc tatcccgttg attcgtagcg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcaacag tgaactttta cctgaaccgc tgcgaccaac
     3841 caacacggca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc accggatcgg tcgatgctaa
     3961 ttccacctcg ccacgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcacca acagcttggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca cgccagtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaatgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaac aacggaatta acggtgcagt gcccagcaga atgagcgccg cagcccagtt
     4501 tgaggggaag attgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccgcc catgcgcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcacca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt caggggcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccttg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagag tatgaagatg
     7921 aaaatcacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
     8041 tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
     8101 ctgccgaccc tgacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc ggcacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagccacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccacagcagc
     8461 cgtattatgc acctgcagct gaacaacctg tgcaacaacc ttactacgct cctgcggcgg
     8521 aacaacctgt tcagcaaccg tattacgccc ctgcgccaga acaaccggtg gcaggtaacg
     8581 cctggcaagc cgaagagcag caatccactt ttgctccaca gtctacatac cagactgagc
     8641 aaacttatca gcaaccagcc gctcaggagc cgttgtacca acagccgcaa cccgttgaac
     8701 agcagcctgt tgtggagcct gaacccgttg tagaagagac aaaacccgcg cgtccgccac
     8761 tttactattt tgaagaagtg gaagagaagc gagcccgtga acgtgaacaa cttgcggcct
     8821 ggtatcaacc gattccagaa ccggttaaag aaccagaacc gatcaaatct tcgctgaaag
     8881 caccttctgt tgcagcagta cctccagtag aaaccgctgc cgctgtttct ccgctggcat
     8941 ctggcgtgaa aaaagcgaca ctggcgacgg gggctgccgc aaccgttgcc gcgccagtct
     9001 tcagtctggc aaatagcggt ggaccgcgtc cgcaggtcaa agaggggatt ggcccgcagt
     9061 tgccacgacc gaaacgtatc cgcgtgccaa ctcgccgtga attggcgtct tacggtatta
     9121 agctgccctc acagcgtgcg gcggaagaaa aagcccgtga agcccagcgc aatcagtacg
     9181 attctggcga tcagtacaac gatgatgaaa tcgatgcgat gcagcaggat gaactggcac
     9241 gtcagttcgc ccagacacag cagcaacgct atggcgaaca gtatcaacat gatgtgcccg
     9301 taaacgcaga agatgcagat gctgcggcag aggctgaact ggctcgtcag tttgcgcaaa
     9361 ctcaacaaca acgttattcc ggcgaacaac cggctggggc gaatccgttc tcgctggatg
     9421 attttgaatt ttcgccaatg aaagcgttgc tggatgatgg tccacacgaa ccgttgttta
     9481 cgccaattgt tgaacctgta cagcagccgc aacaaccggt tgcaccgcag cagcagtatc
     9541 agcagccaca acagccggtt gcgccacagc agcaatatca gcagccgcaa cagccggttg
     9601 cgccgcagcc acaatatcag cagccgcaac aaccggttgc gccacagcag caatatcagc
     9661 agccgcaaca accagttgcg ccgcagccgc agtatcaaca gccgcaacaa ccggttgcgc
     9721 cgcagcagca gtatcagcag ccacaacagc cagttgcgcc acaaccgcag gataccctgc
     9781 ttcatccgct gttgatgcgt aatggcgaca gccgtccgtt gcataaaccg acgacgccgc
     9841 tgccttctct ggatttgctg acaccgccgc cgagcgaagt ggagccggta gatacctttg
     9901 cgcttgaaca aatggctcgc ctggtggaag cgcgtctggc tgatttccgt attaaagccg
     9961 atgttgtcaa ttactctccg gggccggtta tcactcgctt tgaattgaac ctggcaccgg
    10021 gcgtaaaagc ggcgcgcatt tctaacttgt cacgggacct tgcccgttca ctttcgacgg
    10081 tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta tgtaggtctg gagttaccga
    10141 ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga taacgccaaa ttccgcgata
    10201 atccgtcgcc attaaccgtg gtgctgggta aagatatcgc cggtgagccg gtggttgccg
    10261 atctggcgaa aatgccgcac ttgttggttg cggggactac cggttccggt aaatctgtcg
    10321 gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca gccagaagat gtgcgtttca
    10381 tcatgatcga cccgaaaatg ctggagcttt cggtttatga aggcattccg catctgttaa
    10441 cggaagtcgt tactgatatg aaagatgccg ccaacgcgct gcgctggtgt gttaacgaga
    10501 tggagcgtcg ttataaactg atgtctgcgc tgggtgtgcg taatctggcg ggttataacg
    10561 aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc agacccgtac tggaagccgg
    10621 gtgacagtat ggatgcccag catccggtgc tgaaaaaaga accatacatt gtggtgttgg
    10681 ttgacgaatt tgccgacctg atgatgacgg taggtaaaaa agtggaagag ctgatagcac
    10741 gtctggcgca aaaagcccgt gccgcgggta tccacctcgt actggcaact cagcgtccat
    10801 cggttgatgt tattactggt ctgattaaag cgaatattcc gacccgtatc gcctttaccg
    10861 tatccagtaa gattgactca cgtaccattc ttgatcaggc tggcgcggaa tcactgctgg
    10921 ggatggggga tatgctctac tctgggccga actccacgtt gccggtacgt gtccatggtg
    10981 cttttgttcg cgatcaggaa gttcatgccg tggtgcagga ctggaaagcg cgtggtcgcc
    11041 cacagtatgt tgatggcatc acttccgaca gtgaaagcga aggtggcgcg ggcggtttcg
    11101 atggcgctga agaactggat ccgttgttcg atcaggcggt gcagtttgtc actgaaaaac
    11161 gcaaagcgtc aatttctggc gtacagcgtc agttccgcat tggttataac cgtgcagcgc
    11221 gtattatcga acagatggaa gcgcagggga ttgtcagcga acaggggcac aacggtaatc
    11281 gtgaagtgct ggctccaccg ccgtttgact aattaatgca ttgccggata aggcgcggta
    11341 gcgtcgcatc cggcactcta tcaactgaaa attcagtatt ttcttctttc ctcaagctga
    11401 ttattagcct ggaatagaga gtagagggaa ctcccgatcg ggagtgacgt aatttgagga
    11461 ataatgatga aaaaaattgc catcacctgt gcattactct caagcttagt agcaagcagc
    11521 gtttgggctg atgccgcaag cgatctgaaa agccgcctgg ataaagtcag cagcttccac
    11581 gccagcttca cacaaaaagt gactgacggt agcggcgcgg cggtgcagga aggtcagggc
    11641 gatctgtggg tgaaacgtcc aaacttattc aactggcata tgacacaacc tgatgaaagc
    11701 attctggttt ctgacggtaa aacactgtgg ttctataacc cgttcgttga gcaagctacg
    11761 gcaacttggc tgaaagatgc caccggtaat acgccgttta tgctgattgc ccgcaaccag
    11821 tccagcgact ggcagcagta caatatcaaa cagaatggcg atgactttgt cctgacgccg
    11881 aaagccagca atggcaatct gaagcagttc accattaacg tgggacgtga tggcacaatc
    11941 catcagttta gcgcggtgga gcaggacgat cagcgcagca gttatcaact gaaatcccag
    12001 caaaatgggg ctgtggatgc agcgaaattt accttcaccc cgccgcaagg cgtcacggta
    12061 gatgatcaac gtaagtagag gcacctgagt gagcaatctg tcgctcgatt tttcggataa
    12121 tacttttcaa cctctggccg cgcgtatgcg gccagaaaat ttagcacagt atatcggcca
    12181 gcaacatttg ctggctgcgg ggaagccgtt gccgcgcgct atcgaagccg ggcatctgca
    12241 ttctatgatc ctctgggggc caccgggtac cggcaaaaca accctcgctg aagtgattgc
    12301 ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc acctctggcg tgaaagagat
    12361 tcgcgaggcg atcgagcgcg cccggcaaaa ccgcaatgca ggtcgccgca ctattctttt
    12421 tgttgacgaa gtgcaccgat tcaacaaaag ccagcaggat gcatttctgc cacatattga
    12481 agacggcacc atcactttta ttggcgcaac cactgaaaac ccgtcgtttg agcttaattc
    12541 ggcactgctt tcccgtgccc gtgtctatct gttgaaatcc ctgggtacag aggatattga
    12601 gcaagtacta actcaggcga tggaagacaa gacccgtggt tatggtggtc aggatattgt
    12661 tctgccagat gaaacacgac gcgccattgc tgaactggtg aatggcgacg cgcgccgggc
    12721 gttaaatacg ctggaaatga tggcggatat ggccgaagtc aatgatagcg gtaagcgggt
    12781 cctgaagcct gaattattga ccgaaatcgc cggtgagcga agcgcccgct ttgataacaa
    12841 aggcgatcgc ttttacgatc tcatttccgc actgcataag tcggtacgtg gtagcgcacc
    12901 cgatgcggcg ctctactggt atgcgcgaat tattaccgct ggtggcgatc cgttatatgt
    12961 cgcgcgtcgt tgcctggcaa ttgcttccga agatgtcggc aatgccgacc cacgggcgat
    13021 gcaggtggca attgcggcct gggattgctt tactcgcgtt ggcccggcgg aaggtgaacg
    13081 cgccattgct caggcgattg tttacctggc ctgcgcgcca aaaagcaacg ctgtctacac
    13141 tgcgtttaaa gctgcgctgg cggatgctcg cgaacgcccg gattatgacg tgccggttca
    13201 tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc tacgggcagg aatatcgtta
    13261 cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt tacttcccgc cggaaatagc
    13321 acaaacacgc tattatttcc cgacaaacag gggccttgaa ggcaagattg gcgaaaagct
    13381 cgcctggctg gctgaacagg atcaaaatag ccccataaaa cgctaccgtt aatgttatcg
    13441 ttgcggtaat gttgttactg tatccctgtg gtcgcaggct gtggccacat ctcccattta
    13501 attcgataag cacaggataa gcatgctcga tcccaatctg ctgcgtaatg agccagacgc
    13561 agtcgctgaa aaactggcac gccggggctt taagctggat gtagataagc tgggcgctct
    13621 tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac ctgcaagcgg agcgtaactc
    13681 ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa gatatcgagc ctttacgtct
    13741 ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa gccgagctgg atgctttaca
    13801 ggctgaaatt cgcgatatcg cgctgaccat ccctaacctg cctgcagatg aagtgccggt
    13861 aggtaaagac gaaaatgaca acgttgaagt cagccgctgg ggtaccccgc gtgagtttga
    13921 ctttgaagtt cgtgaccacg tgacgctggg tgaaatgcac tctggcctcg actttgcagc
    13981 tgcagttaag ctgactggtt cccgctttgt ggtaatgaaa gggcagattg ctcgcatgca
    14041 ccgcgcactg tcgcagttta tgctggatct gcataccgaa cagcatggct acagtgagaa
    14101 ctatgttccg tacctggtta accaggacac gctgtacggt acgggtcagc tgccgaaatt
    14161 tgctggcgat ctgttccata ctcgtccgct ggaagaagaa gcagacacca gtaactatgc
    14221 gctgatccca acggcagaag ttccgctgac taacctggtg cgcggtgaaa tcatcgatga
    14281 agatgatctg ccaattaaga tgaccgccca caccccatgc ttccgttctg aagccggttc
    14341 atatggtcgt gacacccgtg gtctgatccg tatgcaccag ttcgacaaag ttgaaatggt
    14401 gcagatcgtg cgcccggaag actcaatggc ggcgctggaa gagatgactg gtcatgcaga
    14461 aaaagtcctg cagttgctgg gcctgccgta ccgtaaaatc atcctttgca ctggcgacat
    14521 gggctttggc gcttgcaaaa cttacgacct ggaagtatgg atcccggcac agaacaccta
    14581 ccgtgagatc tcttcctgct ccaacgtttg ggatttccag gcacgtcgta tgcaggcacg
    14641 ttgccgcagc aagtcggaca agaaaacccg tctggttcat accctgaacg gttctggtct
    14701 ggctgttggt cgtacgctgg ttgcagtaat ggaaaactat cagcaggctg atggtcgtat
    14761 tgaagtacca gaagttctgc gtccgtatat gaacggactg gaatatattg gctaataccc
    14821 aatttttctg aatctaaaaa gcgcctgcgg gcgctttttt tgtctccctt tgataccgaa
    14881 caataattac tcctcactta cacgtaatac tactttcgag tgaaaatcta cctatctctt
    14941 tgattttcaa attattcgat gtatacaagc ctatatagcg aactgctata gaaataatta
    15001 cacaatacgg tttgttactg gaatcaatcg tgagcaagct tgagtgagcc attatgaaaa
    15061 cgaaaatccc tgatgcggta ttggctgctg aggtgagtcg ccgtggtttg gtaaaaacga
    15121 cagcgatcgg cggcctggca atggccagca gcgcattaac attacctttt agtcggattg
    15181 cgcacgctgt cgatagcgcc attccaacaa aatcagacga aaaggttatc tggagcgcct
    15241 gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca cgtcgtggac ggtgaaatca
    15301 aatatgtcga aacggacaat accggcgatg acaattacga cggcctgcac caggttcgcg
    15361 cctgcctgcg tgggcgttcc atgcgtcgcc gtgtctacaa tccggaccgc ctgaaatatc
    15421 cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga gcgcattagc tgggaagaag
    15481 cctacgacat catcgcgacc aatatgcagc gcctgatcaa agagtacggc aacgagtcta
    15541 tctatctgaa ctatggcacc ggtacgctgg gcggcaccat gacccgctcc tggccgccgg
    15601 gaaataccct ggtcgcgcgg ctgatgaact gctgcggcgg ctatctgaac cattacggcg
    15661 actactcctc cgcgcaaatt gcggaaggtt tgaactatac ctacggcggc tgggcagatg
    15721 gcaacagccc gtcggatatc gaaaacagta agctggtagt gctgtttggt aataaccctg
    15781 gcgaaacgcg aatgagtggc ggtggggtga cttactatct tgaacaggca cgccagaaat
    15841 ctaatgcccg catgatcatc atcgatccgc gctataccga caccggtgcc gggcgcgaag
    15901 atgagtggat ccctattcgt ccgggaacag atgccgcact ggttaacggt ctggcgtacg
    15961 tcatgatcac tgaaaacctg gtggatcagg cattcctcga taaatattgc gttggctacg
    16021 atgagaaaac cctgccagcc agtgcgccga aaaatggcca ctataaagct tatattctgg
    16081 gtgaagggcc agatggcgtg gctaaaacac cggaatgggc ctcacaaatc accggtgttc
    16141 cggcagacaa aatcatcaaa ctggctcgtg aaatcggcag taccaaaccg gcgtttatca
    16201 gccagggatg gggcccgcag cgtcacgcta acggtgaaat cgcaacccgt gctatctcga
    16261 tgctggcgat tctgaccggt aacgttggta ttaatggagg gaacagcggc gcgcgtgaag
    16321 gttcatacag cttaccgttt gtccgtatgc cgaccttgga aaacccgatc cagaccagca
    16381 tttcgatgtt tatgtggacc gatgccattg aacgtggccc ggaaatgacg gcgctgcgtg
    16441 atggtgtacg cgggaaagat aagctggatg tgccgatcaa aatgatctgg aactatgccg
    16501 gtaactgcct ggttaaccag cattctgaaa tcaaccgtac ccatgaaatc cttcaggatg
    16561 ataagaagtg cgagctgatt gtggttatcg actgccacat gacctcatcg gcgaaatatg
    16621 ctgacatcct gctgcctgac tgcaccgctt ccgaacagat ggactttgcg ctggatgcat
    16681 cctgcgggaa tatgtcttac gtgattttca acgatcaggt gattaaaccg cgctttgaat
    16741 gtaagaccat ctatgaaatg accagcgaac tggcaaaacg tcttggcgtt gagcaacagt
    16801 ttactgaagg ccgtacccag gaagagtgga tgcggcatct gtatgcccag tcgcgggaag
    16861 cgattcctga actgccaacg tttgaagagt tccgcaagca ggggatcttt aaaaagcgcg
    16921 acccacaagg gcatcacgtt gcttataaag ccttccgtga agatccgcag gcaaacccac
    16981 tgactacgcc atcgggcaaa attgagattt attcgcaggc gctggctgac attgccgcta
    17041 cctgggaatt gccagaaggc gatgtgatcg atccactgcc gatctacacg ccgggctttg
    17101 aaagttatca ggatccgctg aacaaacagt atccgctgca gcttacgggt ttccactata
    17161 aatctcgcgt tcactcaact tacggcaacg ttgatgtgct gaaagcagct tgccgtcagg
    17221 aaatgtggat caacccgctt gatgcgcaaa aacgcggtat caacaacggc gataaagtca
    17281 ggatctttaa cgatcgtggt gaagttcata ttgaggcgaa agtgacgcca cgaatgatgc
    17341 caggtgtggt cgcactgggt gaaggtgcct ggtatgaccc ggatgcaaaa cgtgtcgata
    17401 agggtggttg tattaacgta ctgaccactc aacgtccgtc tcctctcgct aaggggaatc
    17461 cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg agtaaccgat gacaacccag
    17521 tatggatttt ttattgattc cagccgttgc accggttgca aaacctgcga gctggcttgt
    17581 aaagactaca aagatttgac gccagaagtc agcttccgcc gcatttatga atatgctggc
    17641 ggcgactggc aggaagataa cggtgtctgg caccagaacg tgtttgccta ctatctgtcg
    17701 atttcgtgca accactgcga agatccggct tgtactaaag tctgcccgag cggtgcgatg
    17761 cataaacgtg aagatggttt tgttgtggtc gatgaagatg tgtgcattgg ctgccgctac
    17821 tgccatatgg cttgcccgta tggcgcaccg caatataacg aaacgaaagg ccatatgacc
    17881 aaatgcgatg gttgttatga ccgtgttgcg gagggtaaaa agccgatctg tgttgaatct
    17941 tgtccgctgc gggcgctgga tttcggccct atcgacgagc tgcgtaaaaa acatggcgat
    18001 ctggcggccg ttgcgccgtt gccgcgagct cactttacca aaccgaatat tgtgatcaaa
    18061 cccaatgcca atagccgccc gaccggggat accactggct atctggcaaa cccgaaggag
    18121 gtgtgagatg ggaagtggat ggcatgaatg gccgctgatg atcttcacgg tcttcgggca
    18181 atgtgtggcg ggtggtttta tcgttctggc tttggcgctg ctcaaaggcg acctgcgagc
    18241 agaagcccag cagcgtgtta tcgcctgcat gtttggctta tgggtgctga tgggcattgg
    18301 ctttatcgct tctatgctcc atcttggttc accgatgcgc gcttttaact cgctcaaccg
    18361 ggtaggggct tcagcactca gtaacgaaat cgccagcggt tcgatctttt ttgccgtagg
    18421 cggcatcggc tggttgctgg caatgctgaa aaagctgtca ccggcattgc gtacgctttg
    18481 gctggtggtg acgatggttc ttggcgtcgt ctttgtctgg atgatggtgc gtgtgtataa
    18541 cagcattgat accgttccga cctggtacag catctggacg ccgatgggct tcttcctgac
    18601 gatgttcatg ggcggcccgt tactggggta cctgttgctg agtctggctg gcgtcgatag
    18661 ctgggcgatg cgtctgctgc cagccatttc cgtactggca ctggtggtga gtggcgtggt
    18721 gtcagtgatg cagggcgcag agctggcgac tattcatagt tctgtgcagc aggccgcagc
    18781 gctggtgccg gactatggtg cgctgatgtc ctggcggatc gtgcttttgg ccgttgccct
    18841 gtgcttgtgg attgcaccac agctaaaagg ttatcagcct gcggttccgc tactatccgt
    18901 ctcattcatt ctgcttctgg caggggaatt aatcggtcgc ggcgtattct atggtttgca
    18961 tatgaccgtg gggatggccg tcgcaagcta atcagtaaca accggggttt cggccccggt
    19021 tctcttttat ttctgcttcg ttaacgtgtc ataactggtc atcaaattac gataatcagg
    19081 aatatggtta gagaacaacg tcgccagtcc ttcaatatca ttacgccagt cacggtgcag
    19141 ctcgcaagcc acgccgaacc atgtcatcaa ttgcgcacct gcttgcgaca tgcgatccca
    19201 tgccgaatgc cgggtaattt cattaaaggt ccctgacgcg tcagtcacaa caaacacatc
    19261 aaacccttct tcaatggctg aaagcgccgg gaatgccacg caaacttcgg ttaccacacc
    19321 ggcaataatt aactgttttt tacctgtcgc tttgactgct tttacaaaat cttcgttatc
    19381 ccaggcgtta atattgcccg ggcgagcaat gtaaggtgca tcggggaatt gtgctttcag
    19441 ttctggaact aatgggccgt taggaccggt ttcaaaactg gtggtaagaa tagttggtaa
    19501 gttgaaatac ttagccaggt cacccagcgc cagcacgtta tttttaaact tatcgggttc
    19561 gatatcccgt acaagggaaa gtaaaccagc ctggtgatca acaagcaaaa cagcagcatc
    19621 atttttatca agacgaacat acggtttggt catcctcttc tcctttcgag agtcagtggt
    19681 acgcctgaaa tcggtcagac gtgatgcatt gctctgaaag catagacggg aaatatgagt
    19741 ttgctgtgac catgaaattt ttcgactgaa cgaagcgtat gaaatttgtg ttagatcaat
    19801 aaaaacaatc agatgagatt aatcatctct tcagtattag cgttatgaat gagtaatata
    19861 aatcgataat accgccgttg cgatatgcgc gtattttcac ccattgacaa tgtttttggc
    19921 ggtggcatga tgcgcatgaa atttgaactt cctcacggtt ttaattcatg tccacgtata
    19981 cccggcctgt catgcttttg ctgtctggcc tgcttttgtt gactctggcg atcgcggtgt
    20041 taaatacact cgtgccgctt tggctcgccc aggaacacat gtccacatgg caggtaggcg
    20101 ttgtcagctc atcctatttt accggcaacc ttgtcggtac attgctgaca gggtatgtca
    20161 ttaaacgcat tggttttaac cgcagctatt atctggcctc cttcattttt gccgctggct
    20221 gtgccggcct tggcctgatg attggattct ggagttggtt ggcgtggcgt tttgtcgcgg
    20281 gcgtcggctg tgccatgatt tgggtagtcg ttgagagcgc gctgatgtgc agtgggacgt
    20341 cacgtaaccg tgggcgtttg cttgctgcat acatgatggt ttattacgtg ggaacgtttt
    20401 taggccagtt actggtcagc aaggtctcaa ccgagctgat gagcgtattg ccgtgggtta
    20461 caggtttgac attggcaggg atcttaccgc tgttgtttac gcgtgtcctg aatcagcagg
    20521 ctgaaaacca tgattcgacg tcaattacgg caatgctaaa actccgtcag gcgcggcttg
    20581 gcgtgaatgg ctgcattatc tcaggaatcg ttctgggatc tctatatggc ctgatgcctc
    20641 tgtacctcaa tcacaaaggg gtgagcaatg ccagtattgg tttctggatg gcggtactgg
    20701 tcagtgcggg tatcctcgga caatggccga ttggacgtct ggcggataag tttggtcgac
    20761 tgctggtttt gcgtgttcag gtctttgtcg tcattctcgg cagtattgcg atgcttagcc
    20821 aggcggcgat ggccccagcg ttattcatcc tcggtgccgc tggctttacg ctatatccgg
    20881 tggcgatggc atgggcttgc gagaaagttg aacatcatca actggtggcg atgaaccaag
    20941 ccttactgtt gagctatact gtgggaagtc tgcttggccc gtcatttacc gctatgctaa
    21001 tgcagaattt ctccgataat ttattgttta tcatgatcgc cagcgtatcg tttatctatt
    21061 tgctgatgct gctgcgcaac gccggtcata cgccgaaacc cgttgctcac gtgtaaatga
    21121 attcaagcag agtgtgaact tactgtttca cactctgctt ttttgtttct tctatctgac
    21181 ttgctttatt ccaaatttta ttcgtttaaa aataaaatgt gtagcaggtt ataattttgc
    21241 atttcgctat ttccgcactt cttatttgcc gcgcataatc cctcgtttta cagatgcccc
    21301 tataattttg gcgaaggatt tgtctatggc tgggaatgtt caggaaaaac agctgcgatg
    21361 gtacaacatt gcgctgatgt cttttatcac tgtctggggt tttggcaacg ttgttaataa
    21421 ctacgccaac caggggctgg tggttgtttt ttcatgggtg tttatctttg cgctctattt
    21481 cacaccttat gcgctaattg ttggtcagtt aggctcgacc ttcaaagatg ggaagggcgg
    21541 ggtcagtacc tggattaaac acacgatggg acccggactg gcttatctcg ccgcgtggac
    21601 ctactgggtg gtgcatattc cctatctggc acaaaaaccc caggcaattc tgattgcgct
    21661 cggttgggcg atgaaaggcg acggttcgct aatcaaagaa tattcagtcg tagcgttaca
    21721 ggggttaacg ctggtgctgt ttatcttctt tatgtgggtt gcttcacgcg gtatgaaatc
    21781 gctgaaaatc gtcggttctg tggcagggat tgctatgttt gttatgtcgc tcctgtatgt
    21841 ggcgatggcg gtaaccgcgc ctgcaattac tgaagtgcat attgcgacca caaacattac
    21901 ctgggaaacg ttcattcctc atatcgactt tacctacatt accactattt caatgctggt
    21961 tttcgcggtt ggcggagcag agaagatttc tccttacgtt aatcaaacgc gcaacccagg
    22021 aaaagaattt ccaaaaggga tgttatgcct ggcggtgatg gttgcggttt gtgccattct
    22081 gggctcgctg gcgatgggga tgatgtttga ttcgcgtaat atcccggatg acttaatgac
    22141 caacggtcag tattacgcct ttcagaagct gggcgagtat tacaacatgg gtaatacttt
    22201 aatggtgatt tacgccattg cgaataccct gggacaagta gcggcgctgg tattctcgat
    22261 tgatgccccg cttaaagtgc tattaggtga tgctgacagc aaatatattc cagccagttt
    22321 atgtcgtacc aacgcttctg gtacgcccgt taatggctat tttctgaccc tggtactggt
    22381 ggcgattctg attatgctgc cgactctcgg cattggtgat atgaacaatc tctataaatg
    22441 gctgttgaac cttaattcgg tagtgatgcc gctgcgttat ttatgggtat ttgttgcatt
    22501 tattgcagtc gttcgcctgg cgcagaaata taaaccagaa tatgtcttta ttcgtaataa
    22561 gcctctggca atgaccgtcg ggatttggtg ttttgccttt accgcctttg cctgtttgac
    22621 ggggatcttc ccgaaaatgg aagccttcac tgcagagtgg accttccagt tggcgctgaa
    22681 tgttgcaacg ccgtttgtgc tggtaggatt aggactgata ttcccgctgc tggcgcgtaa
    22741 agcgaatagt aaataattat tgtggtcggt gacgctgact ctggcagatc tgacccgttt
    22801 ttacataatc gagaaatgcg cgcaagccgc aggacatata ctggcggttt gggtaataga
    22861 tctggaagcc tggacgctct gtgatccagt cttccagtac acattctaaa cgaccggtat
    22921 caaggtactc cttgatctgt tcatagagca gatacccgat accagcaccc atcaaaacgg
    22981 catccagttc agcatcaaca tcatc
//
LOCUS       DAZRUF010000043        22848 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZRUF010000043 DAZRUF010000000
VERSION     DAZRUF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22848
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.043"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            120..749
                     /locus_tag="ACHXOL_001355"
     CDS             120..749
                     /locus_tag="ACHXOL_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR6808375.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            776..2437
                     /locus_tag="ACHXOL_001356"
     CDS             776..2437
                     /locus_tag="ACHXOL_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR6808376.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            2676..2735
                     /locus_tag="ACHXOL_001357"
     CDS             2676..2735
                     /locus_tag="ACHXOL_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR6808377.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3052..3111
                     /gene="ibsE"
                     /locus_tag="ACHXOL_001358"
     CDS             3052..3111
                     /gene="ibsE"
                     /locus_tag="ACHXOL_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR6808378.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3231..4664)
                     /gene="hldE"
                     /locus_tag="ACHXOL_001359"
     CDS             complement(3231..4664)
                     /gene="hldE"
                     /locus_tag="ACHXOL_001359"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR6808379.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(4712..7552)
                     /gene="glnE"
                     /locus_tag="ACHXOL_001360"
     CDS             complement(4712..7552)
                     /gene="glnE"
                     /locus_tag="ACHXOL_001360"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR6808380.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7575..8876)
                     /gene="ygiF"
                     /locus_tag="ACHXOL_001361"
     CDS             complement(7575..8876)
                     /gene="ygiF"
                     /locus_tag="ACHXOL_001361"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR6808381.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9118..9738
                     /locus_tag="ACHXOL_001362"
     CDS             9118..9738
                     /locus_tag="ACHXOL_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR6808382.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9802..11040
                     /gene="cca"
                     /locus_tag="ACHXOL_001363"
     CDS             9802..11040
                     /gene="cca"
                     /locus_tag="ACHXOL_001363"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR6808383.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11203..12024)
                     /gene="bacA"
                     /locus_tag="ACHXOL_001364"
     CDS             complement(11203..12024)
                     /gene="bacA"
                     /locus_tag="ACHXOL_001364"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR6808384.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12115..12483)
                     /gene="folB"
                     /locus_tag="ACHXOL_001365"
     CDS             complement(12115..12483)
                     /gene="folB"
                     /locus_tag="ACHXOL_001365"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR6808385.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12588..13205
                     /gene="plsY"
                     /locus_tag="ACHXOL_001366"
     CDS             12588..13205
                     /gene="plsY"
                     /locus_tag="ACHXOL_001366"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR6808386.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13218..14150)
                     /gene="ttdR"
                     /locus_tag="ACHXOL_001367"
                     /gene_synonym="dan"
     CDS             complement(13218..14150)
                     /gene="ttdR"
                     /locus_tag="ACHXOL_001367"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFR6808387.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14357..15268
                     /gene="ttdA"
                     /locus_tag="ACHXOL_001368"
     CDS             14357..15268
                     /gene="ttdA"
                     /locus_tag="ACHXOL_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR6808388.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15265..15870
                     /gene="ttdB"
                     /locus_tag="ACHXOL_001369"
     CDS             15265..15870
                     /gene="ttdB"
                     /locus_tag="ACHXOL_001369"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR6808389.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15919..17382
                     /gene="ttdT"
                     /locus_tag="ACHXOL_001370"
     CDS             15919..17382
                     /gene="ttdT"
                     /locus_tag="ACHXOL_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR6808390.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17425..18438)
                     /gene="tsaD"
                     /locus_tag="ACHXOL_001371"
     CDS             complement(17425..18438)
                     /gene="tsaD"
                     /locus_tag="ACHXOL_001371"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR6808391.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            18675..18890
                     /gene="rpsU"
                     /locus_tag="ACHXOL_001372"
     CDS             18675..18890
                     /gene="rpsU"
                     /locus_tag="ACHXOL_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR6808392.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19001..20746
                     /gene="dnaG"
                     /locus_tag="ACHXOL_001373"
     CDS             19001..20746
                     /gene="dnaG"
                     /locus_tag="ACHXOL_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR6808393.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20941..22782
                     /gene="rpoD"
                     /locus_tag="ACHXOL_001374"
     CDS             20941..22782
                     /gene="rpoD"
                     /locus_tag="ACHXOL_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR6808394.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5536 a         6235 c         5842 g         5235 t
ORIGIN      
        1 taaaaaactt tctcttccag aacttctcta acatgtgtta ttattaacaa agataaatgt
       61 aagcaaggaa actatcccaa agtaaatatt gcgtcactaa atggacattg gagtgacata
      121 tgattttatt cgccgactat aatacccctt atcttttcgc tatttccttt gtactgttga
      181 tcggcctttt agaaatttta gcgcttatct gcggtcacat gctctccggt gctcttgatg
      241 cacatcttga ccattataat tccataacca caggtcatat tagccaggca cttcattatc
      301 tcaatatcgg aagattgcca gctctcgtcg ttctctgctt gctggctggt ttttttggtc
      361 ttattggcat cctgttgcag catgcctgca tcatggtctg gcaatcacca ctgtcgaacc
      421 tgttcgtggt tcctgtcagt ttactgttta cgattattgc ggtgcattat accggtaaaa
      481 tagttgcgcc ctggattcca cgcgaccata gttcagctat tacagaagaa gaatatattg
      541 gtagcatggc attaattacc ggtcatcaag ccacttcagg taatccctgc gaaggaaaac
      601 tcaccgatca atttggccaa atacattatt tactactgga gcctgaagaa ggaaaaattt
      661 tcactaaagg agtcaaagtg ttaattattt gccgactttc tgcaacgcga tatttggcgg
      721 aaaacaatcc ctggccacaa atattgtaat ttattaatca aaaggaaatt taataatgga
      781 tgatattgtt aattctgtgc cctcctggat gtttaccgcg attattgccg tatgcattct
      841 gtttattatt ggaattattt tcgccaggct ctatcgtcgc gcttcggcag agcaagcttt
      901 tgttcgtact ggtttaggtg ggcaaaaagt ggtgatgagc ggtggcgcaa tcgtgatgcc
      961 gatctttcat gaaataatcc ccatcaatat gaatactctg aagctggaag tcagccgctc
     1021 aaccattgat agcctgatta cgaaagatcg tatgcgtgtc gatgtagtag tcgctttctt
     1081 tgtacgggta aaaccgtctg tagaaggcat cgccactgcg gcgcagacgt tggggcaacg
     1141 cacgctatca ccagaagact tacgtatgtt ggttgaagat aaatttgtcg atgccctccg
     1201 tgcaacagct gcacaaatga ccatgcatga gttacaggat acccgcgaga acttcgtgca
     1261 gggagtacaa aatactgtcg cagaagacct atcgaaaaac ggtcttgagc tggaaagcgt
     1321 ttcacttacc aactttaacc agacctcgaa agaacatttc aatccgaaca atgcctttga
     1381 cgccgaaggt ttaaccaaac tgactcagga gacggagcgc cgtcgtcgcg aacgtaacga
     1441 agttgaacag gatgtagaag ttgcggtgcg tgagaaaaac cgtgatgcac tttcgcgcaa
     1501 gttggagatt gaacaacaag aagcgtttat gacgcttgag caggagcagc aggttaaaac
     1561 ccgtactgcc gaacagaatg cacgtattgc ggcttttgaa gctgaacgtc gtcgtgaagc
     1621 agagcagacg cgaattctgg ctgaacgaca aattcaggaa acggagatcg agcgcgagca
     1681 ggccgtccgc tcaagaaagg ttgaggctga acgtgaagtt cgtattaaag agatcgaaca
     1741 gcagcaggtc accgaaatcg ccaaccagac gaaatcgatc gctattgccg ccaaatcgga
     1801 acaacagtcc caggcagaag cgcgtgctaa ccttgcactc gcagaagcag taagtgcgca
     1861 acaaaacgta gaaaccactc gccagaccgc agaagccgat cgtgctaaac aagttgccct
     1921 aatcgctgcc gcgcaggatg cagaaaccaa agcggttgaa ctgaccgtgc gggcgaaagc
     1981 agaaaaagaa gccgcagaga tgcaggcggc ggctatcgtt gagttagccg aagctacacg
     2041 taaaaagggt ctggcggaag cagaagcaca acgtgcgctg aacgatgcta tcaacgtact
     2101 ttctgatgaa caaaccagcc ttaaattcaa actggccttg ttgcaggcgc tacctgcggt
     2161 aatagagaaa tccgttgagc cgatgaagtc cattgacggt atcaagatta ttcaggtcga
     2221 tggtctgaat cgtggcagcg ctgcgggtga tgcaaacacg ggtaatgtgg ggggcggaaa
     2281 cctagcggaa caagcattat cagccgctct ctcttaccgc acacaggcac cgctgattga
     2341 ctccttgctc aatgaaattg gcgtttcagg cggctcactg gcggcattga cttcaccctt
     2401 aacctcaaca actcccgtcg ccgaaaacgt agaataaatt ttcagctatg aacgtccctg
     2461 gcacaaaaag ccggggattt tttatatctg cgttccgcta aaaactgcaa atgatcaggc
     2521 cgttgcaacg ttttgcgtga ccgctcgggg aaggcaaaac tgcctctggg aaagcgttgc
     2581 gcgaggtccg gcgctcatca acaatcgggg agtcagcaaa gggctgaaac gggaaagccc
     2641 ctcccgagga aggggcctta cagaaggaaa gggttatgat gaagctcgtc atcatactga
     2701 ttgtgttgtt actcgtaagt ttcgcagctt attaacagcc aatcagaggg gggagaaatc
     2761 ctccctcacc cttgttcctt tactttaggt tgaaaaaaaa tcagcgtcaa gaaggtttca
     2821 ttaacacata tccctggccc aaaaagccgg ggatttttta tatctgcgtt ccgctaaaag
     2881 gtgcaaatgc tcaggacgtt gcagcgtttt gcgtgatcgc tcggggaagg caaaactgct
     2941 tcagggaaag cgttgcgcgg ggtccggcgc tcatcaacaa tcggggagtc agcaaggggc
     3001 tgaaacggga aagcccctcc cgaggaaggg gccttataga aggaaagggt tatgatgaag
     3061 ttcgtcatca tactggttgt gttgttactg ttaagtttcc cgacttacta acaactgatc
     3121 agagggggaa gaaatcctcc cttacccttg ttcctttact ttaggttgaa aaaacaacag
     3181 cgtcaatagg cctgcctgct acaaagcgag atctgtgaac cgctttccgg ttagcctttt
     3241 ttatcctgtt ggatcttctt gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc
     3301 accagcactt cgccgccgtt ggcccagact tctttactcc cggcgatctc ttctggttta
     3361 tagtcgccgc ccttcaccag taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg
     3421 tcttcttcaa acgacaccac ccagtcgacc gcttccagtg cgcccagcac aatcatacgc
     3481 tgttcgagcg ggttaacggg gcgggaatcc cctttcagcc gtttggtgga ggcatcgctg
     3541 ttcacagcga caatcagacg gtcacccagt ttgcgagcat ttgccagata ggagacgtgc
     3601 ccggcgtgca ggatgtcaaa gacaccgttg gtcattacca ccttttcgcc gcgtttacgc
     3661 gctgccgcta cggccagctt cagttcctct tcggtcatca cgccaaagcc ggtatctgcg
     3721 cgcccacgca ctgcattttc cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg
     3781 accaccacgc cagccgccgc attggcaaag aagcaggctt cttccagcga attacccgct
     3841 gccagcgttg ccgccaggac gccaatcacc gtgtcgcccg caccggtaac gtcatacact
     3901 tcctgcgcct gggttggcat atgcagcggc gctttacccg gttgcagcag cgacataccc
     3961 tgttcggaac gggtcactaa cagagccgag agttcgtaat cggcaatcag tttcatgccg
     4021 cgctcaacaa tctcttcttc ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga
     4081 ttcggcgtta acagcgtagc gccgcggtag cgctcaaaat cggtaccttt tggatcaatc
     4141 agcaccggaa cgcccgcttt acgcgccagt tggatcatct gctgtacgct tgccagcgca
     4201 cctttggcgt agtcagaaag caccagcgcg ccaatcgaac tcagcgcctg attaatccgc
     4261 tcgtgcagcg gctgcggatc aacgccttcg aaaccttctt cgaaatccag acggatcagc
     4321 tgttggttgc gggaaagtac ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg
     4381 aagtcgcatt tgacgttgac gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca
     4441 atgcccgtca acccgaccag gcgtgcatta gcaccgagag aagcgatatt catcgccacg
     4501 ttagccgcgc cgcccggacg ttcttcgatg gtattcactt taaccacggg caccggcgct
     4561 tccggcgaga tacggctgga gggaccgtac cagtaacgat ccagcattac atcaccaacc
     4621 accatcactc ctgcacgttc aaactctggc agcgttactt tcattcctgt ctcctgagag
     4681 attcaaaatt tgcgcgcgat aataccatac ttcattcttc caccagccac ttctgccagc
     4741 ttgcccgcac cagatcacgc tctgcggtga agcaatcccc cgacacatgg cccggcaatt
     4801 cctgtaatgc cagatgatga agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca
     4861 tcgcttcctg ctcttccata atgtcgtttt gcgccagcag ctccagaata cgcacgttgt
     4921 ctgaccagcg cgttaacttc ggtttttcat gagcgtagcg caacaccaga tattgggtaa
     4981 taaattcgat atcggtaatt ccgccttcat cagctttgat atcaaagcga tcgcgatgtt
     5041 tattgccgag atgagcgcgc attttctcgc gcatttcccg cacttccgtt tgcagagttc
     5101 taccttcacg cggcagcgtc ataatctcgc gacgcactgc gtcaaagtgc gcggtgagct
     5161 gcggatcgcc gtacactacc cgcgcacgca ccagcgcctg atgttcccac gtccaggcct
     5221 cgtttttctg ataatcggca aatgcttctg tggaggtaac cagcattccc gccgccccgg
     5281 acgggcgcag ccgcgcatcc acttcgtaca aaatgccgga agaggttcgc gtgctgaaca
     5341 ggtgcataat gcgttgcgcc agacgaagat aaaactgtcg tccgtcgatt tcccgctcgc
     5401 cgtcagtcat cgcatccatt gggcaatcat ggaggaagat caggtcaaga tcggaactgt
     5461 agcctaactc ccagccgccc agcttgccgt agccgaccac tgcaaaacca cgcccttcgc
     5521 gatcgttgag gtgattcggc ttaccgtagc gggcaaccat ttgaacccac gcctgctgaa
     5581 cgacggcatc tatcatggct tccgccagcc aggttaagtg atcgctcact ttcatcaccg
     5641 gtagcgtacc agcgatatcc gctgcggcga tgcgtaacag ctgcgcctgt ttgaactgac
     5701 gcagcgcctc aagctgttgc tcttcgtcat cttccggcac gcgcagcaaa tactggcgca
     5761 actcatcgcg gtaggcatcg gtcgccgtcg gctggtaaag agtgtttgga tcgagcaatt
     5821 catccagcaa taatggataa cgcgccagct ggctggcaat catcggcgat gcggcacaca
     5881 gagaaatcaa atggttaagc gccgcaggga attcactgag caattctaaa taggtggtgc
     5941 gggtaacaat ccccaccagc aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac
     6001 gcgcacagac atcacttagc agatgcggca tcagatggtc gagcacctga cgtcctcgcg
     6061 gcccgatggt gcgcttatcc agctctttgc ggaaatcgga aatcagcgtt agcacctgtt
     6121 tgcgatcatc ctcgctaaga tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat
     6181 cctgccacag ctcacgccac tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt
     6241 cgccaatcaa ttcattaaac acccggcgca cattgttcat atgtgcggtc agcgccccgg
     6301 tcagttgcgg ccagtcagca aagtccatcg cccacgccag ccgcgcacgg ttaagctcat
     6361 cagaaggaag cgtctgggtt tgttcgtcgt taatgctttg cagcaggttt tccagacgcc
     6421 gcaggaacag atacgccact cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg
     6481 cagcaatggc gctgagcgtt ggcagtaaag agcacgattg cagcgacggt tcgcgcccgc
     6541 cgcgaatgag ctggaatacc tggacgataa attcaatttc gcgaatacca cccgcgccaa
     6601 gtttgatgtt gtcggtcaaa ccgcgccgac gcacttcacg ggcaatcatt cctttcatgt
     6661 tgcgcagcga ctgaatcacg ctgaaatcga tgtaacgacg gaaaacaaac gggcgtagca
     6721 tcgcacgcaa ctcgttagca tagacgcctt cgctatcgcc cataatccgc gctttgacca
     6781 tcgcgtaacg ctcccagtcg cgcccctgct cctggtaata atcctccaac gcggcaaagc
     6841 tcagcaccag cgggccactt tcgccaaacg gacgcagtcg catatccacg cgatagacga
     6901 agccatcctg cgttggttga tccagcactt taatcagccg ctgccccatg cgggtaaaaa
     6961 actgcgcgtt atccagttcc cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa
     7021 aaatcagatc gatatcagag gagaaattca gttccccgcc gcccagctta cccatgccta
     7081 agatcagcag cggttgcgct tcgccctgcg cattgcacgg cgttccccac tcgcggcagc
     7141 aggcgtcata cagccagtca cgcgccgcaa caatcagcgt ctccgccaga taactgagct
     7201 gctgcaatat gctctcttca gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca
     7261 taatgcgccg ccggaatagc cgcagctcgc gcattaaccc ggcttcgtca ctcacattac
     7321 tgagcgcctc ctgcaaccat gcagcgtaat gctgccattc atcggcctgc ggcggttggc
     7381 tttccagttc cgtcaaccac tctcgatgcg caattatgct gtcctgcaca aaatcactga
     7441 aagtaagtac tgacttcgcc tgtgcactta gtggttcctc ggctaaaggc tctggcagcc
     7501 gctcaacaac ggtctgccag tactgctgta acggtgaaga gagcggcttc ataagcgatt
     7561 ttatccttgc ctgattaacg ttttccgctg tgcaaccaga acggttcctg attgtttgcc
     7621 tcattacgga aatgctcaat ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc
     7681 tgccagttct ccagccaggc atgcgcgacg acaggatcat agtaacccgc cagcaacagt
     7741 attgagtcaa tgtcacgcgt caggcgtggc aactggtcac ggtagagatc gcctaacggc
     7801 tggcagaaaa cgcttttcag ttcagcggca tggtgggaaa gatggatatc ggcaaagcgt
     7861 ttgaaggagt cgctgatttt gccctgcgct ttggcatcta aaaatggctg ccatgctttg
     7921 cttaccaacc attcggtcaa cgccagcttc gccattgccg tttcggtaga gtagaccgcc
     7981 gtcacggcag aaaccgcaga agcaatggtc gcttcgcatt gggtcagcaa atcacgtaag
     8041 tgagtgctcg ctttacgcgg cacaatacca ccgaacagca tcagcgtatg acggaccagg
     8101 ctaatggctg ccagcacctg ttctttcgcc gcatcgttac cgcgtaccca cagttcttca
     8161 tgatactgcc attgcgctaa cgccagctcg agcgccgctt ccagcccctg ttctacatcg
     8221 gcttttgccg caacatgcaa aatggtggtc ggtttgattt cacgcgccgg attgccctgc
     8281 gccagatgat agccacgcgc cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat
     8341 accagttggt tcgccagttt cagcaccgcg cgcgtgtcgc cgctaagcag ttccagttcc
     8401 agctcacaga taggttcagc aaattccccc gctttcacct ccccctgatc gagagcgatt
     8461 tcaattcggc tgccatcgac cgccaccagc catttttcgc gataaaaatc ggtgctgaac
     8521 agcggttgta cgcgggaagc gagatcggca ggcaattcac cgttcggcca gacttccgag
     8581 ggaaactgcg ccaggtcgag cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg
     8641 tgtaagccgc ctgtcactct tcctgcaact ttcatggtca tctcatagcg accgttttcg
     8701 ccacgaatac gtaagcccat atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa
     8761 atattcagca actgcacggg gtcatggtgc tcgccgccca gcgtattgag atggtcacgc
     8821 aacgcctcaa cggcactgtg attaacaata aactttaatt cgatttcctg agccatggcc
     8881 tttacttatc ggttatgtca cataagtgac gatgaacggc gaacttaatg cgatcttttt
     8941 tgtcagtaga tagtattttg cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa
     9001 agtttgaagc agtggcaaga cctgacacag aggattccga ttgatgtcga atgctttgcg
     9061 tagcgacact gatgccacta ctatcgttcc actttccatg acaataacga cagcctgatg
     9121 ccaaaattac gcctgatcgg attaacttta ctcgcactta gcgcgactgc cgtctcacac
     9181 gctgaagaaa cgcgctatgt ttctgacgaa ctgaatacct gggtccgtag cggtccggga
     9241 gatcattatc gcctcgtggg cacggttaac gccggcgagg aagtgacctt attacaaact
     9301 gacgccaaca ccaattatgc ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg
     9361 ttgaaacaac ttagcactga gccaagcctg cgctcccgtg tgccagatct ggaaaatcag
     9421 gtcaaaaccc tgaccgataa actcaccaat atcgataaca cctggaatca gcgcaccgca
     9481 gaaatgcagc aaaaagtggc gcagagcgac agcgtgatca acgggttaaa agaagaaaat
     9541 cagaaactga aaaacgagct gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag
     9601 ctggatgaca aacagcgcac catcatcatg caatggttta tgtatggcgg tggcgtgctg
     9661 gggcttggct tgctgctcgg tctggtactg ccgcacctga tcccaagccg caaacgcaaa
     9721 gatcgctgga tgaactaaat cgccttcttc gttgcactgt cttattatta ggaattgtgt
     9781 atttgacgaa gggatgatgg cgtgaagatt tatctggtcg gtggtgctat tcgggatgca
     9841 ttgttagggc taccggtcaa agacagagat tgggtggtag tcggcagtac gccacaggag
     9901 atgctcgacg cgggctacca gcaggtaggc cgcgattttc ctgtgtttct acatccgcaa
     9961 acgcatgaag agtatgcgct ggcacgtacc gaacggaaat ccggttccgg ttacaccgat
    10021 tttacttgct atgccgcacc ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg
    10081 accattaatg cgctggccca ggacgataac ggcgagatta tcgacccgta caacggtctg
    10141 ggcgatctgc aaaatcgtct gttgcgccat gtttcccccg cttttggcga agatccgtta
    10201 cgcgtactgc gcgtggcgcg ttttgctgcg cgttatgccc acctcggttt tcgtattgcc
    10261 gatgaaactc tgacgctgat gcgcgagatg acccatgcgg gtgaactgga acacctgacg
    10321 cctgaacggg tatggaaaga gacggaaagc gccctcacca cccgcaatcc acaggtgttc
    10381 ttccaggtac tgcgcgattg cggcgcactg cgcgttttat tcccggaaat tgacgcactg
    10441 tttggcgttc cggcccctgc caggtggcat ccggaaatcg acacgggtat tcatacctta
    10501 atgacgctct caatggcggc gatgctgagt ccgcaggtcg atgtccgttt cgcaacttta
    10561 tgccacgatc tcggtaaagg actgacgccg ccagaacttt ggccgcgtca tcatggtcac
    10621 ggtccggcgg gtgtgaaatt agtggaacaa ctctgccagc gtctgcgcgt gccaaatgaa
    10681 attcgcgatt tagccagact ggtggctgag tttcacgatc tcatccacac cttcccaatg
    10741 ctgaacccaa aaactatcgt caaattgttt gattccatcg acgcctggcg taaaccgcag
    10801 cgtgtcgagc aactggcgct gaccagcgag gctgacgtac gcggcagaac cggttttgaa
    10861 tcagcggact acccgcaagg ccgttggttg cgcgaagcct gggaagtggc gcagtcagtg
    10921 ccgacaaaag cggtcgttga agcgggattt aaaggtgtgg agattcgcga ggagctgacc
    10981 cgacggcgga ttgcggcggt agccagttgg aaggaacaac gttgcccaaa gcctgactga
    11041 gtataaaact aaacgttaac acgaaccatc ccctcgcccc ttcggggaga gggttagggt
    11101 gaggggaaca gaccagcacc ggtgcgaaca atcaccccca ccccggccct ctccctacag
    11161 agcgagggag aaaaccaggc ctgctcccga accttttctc aatcaaaaga acacgacgta
    11221 caccgcagcc gccacaataa agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat
    11281 caattgcagg aaggttttaa tcgctatcag cgccaccaca aaagcggtga taaacccaac
    11341 ggcaaacatc gagatatcgc cggttgtcag gaagccccag cttttgtaga gatcgagcgc
    11401 cgttgcgccc atcatcatcg gtaccgccag caggaacgag aactcggaag cagcgtaacg
    11461 gctcacgccc atcagcatcc cacctgaaat ggtggccccg gaacgggaga aacccggcca
    11521 cagcgccaga cactggaaac agccaatcat aaacgcctga cgataggtca tatcatcaag
    11581 acccggcgca cgcggctctt tcggcttcag gcattcggcg gcaatcagca acaaaccgcc
    11641 aacgaccagt gcatacatca catttatcgg gttaaacaat gacttaatcg tgtcgtggaa
    11701 caacagcccc agtaccaccg ccggaatcat ccccagcaaa atgtggatca gcgttaaacg
    11761 acctttgctt tctccttcgt gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa
    11821 cagacgccgc cagaacatca ctactaccgc cagaattgat cctaactgga tcacaacttc
    11881 aaaggttttc gccgtgtcgc cctcgaaccc caacaagtga ccgacaataa tcatatggcc
    11941 cgtgctggat accggcagaa attctgtcaa tccttcgacc acacccaata ttgccgctat
    12001 cagcagcgag tgcatatcgc tcatcaataa acccctaaat tattaaaatg taccgcttgt
    12061 ccggactact gcagtaagac caggttataa ccgtttggtt taacagctgt aaaattaatt
    12121 attttctttc agattattgc cacgctcaat gattacacca acattcgccg cccgcgccat
    12181 tgcgcctggc ttgctgagtt tgatgcgcac ccacggcgag ttgaagcgtg ctaacagcag
    12241 ctccgccacc tcttcagcca cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct
    12301 gaccaccgtt tctgcaatgt cagcgtaact gaggcaatcc gccacatcat cacttttcgc
    12361 cgctttacgg ttatcccatg ccatttcgat atcgaacact aacttctgtt cgatggtctg
    12421 ttcccagtcg taaacaccaa tagtggtgat taccgaaagt tgctctataa atacaatatc
    12481 catcacgtcc tgcctgcttt ttggctaacc ggataccact tccggcgaaa tgtgcgtatt
    12541 atccacagat tcatcgttga acacgaattt tcaaaacgga acagcttatg agtgcaatcg
    12601 cgcctggaat gatcctcatc gcgtacctct gcggctccat ttccagtgcc attctggttt
    12661 gccgcttgtg tgggctgccc gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca
    12721 atgtgttacg tatcggtggc aagggagcag ccgtagcagt actgattttc gacgttctga
    12781 aaggaatgtt gcccgtctgg ggcgcgtatg aattaggtgt cagccccttc tggttaggct
    12841 taattgccat cgccgcctgt cttggacaca tctggcccgt tttcttcgga tttaaaggag
    12901 gaaaaggcgt tgctaccgct tttggtgcca tcgcacccat tggctgggat ctcaccggag
    12961 taatggcggg aacctggtta ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga
    13021 ttgtcagtgc actgattgct ccgttttatg tctggtggtt taagccacaa ttcaccttcc
    13081 cggtttcgat gctctcttgc ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt
    13141 ggcgtcgtca ggagacaaaa atctggacga aattcaaaag aaagcgcgaa aaggatcccg
    13201 agtgatttct ggtggatcta catgacctga tagccttcat cgggcttgcc cagccgttgc
    13261 tggcaccatg ccgccagaaa ttccacgcag acacgtaatt tcatgctgcg atagagcggc
    13321 tcccggtaaa cagcccagat attggcgctt tgtgcatact ccggcaatac ttgcaccagt
    13381 ttgccactct ccagaaacgg caacacatcc cactcggaac gcagcataat ccctttgccc
    13441 tccagcgccc attgcaacac tatctcgccg ctattggagg aaagatgccc gctaaccttc
    13501 accgattttt tctcctgacc gttccccaac tcccatattc cgtgggtcat atcgcgctct
    13561 ttggtcacca gacagtcatg acgacttaat tcctgtaatg attgtggctg cggatatttt
    13621 tgcagatact caggtgctgc gcataatatt cttttatttt tcgttaacag atgcgcaata
    13681 taataatcag gaatttcgtc attaatacga atatccagat caatattatc ctgcactaaa
    13741 tcaatttgcc gatcgaacaa ttcaaaatgc acctgtaact caggataatt gcgcataagt
    13801 tcggtaatgg ctggcgcaat atggctgcgc ccaaaaccaa aactgcaacc aatacgaatc
    13861 atcccctccg ggcgcgtttt gatttgcgtg acgtcatcga ccagtcgctg atattgggtg
    13921 aggatttcca gcgcatgttc gtaacaacgt tggccacttt cagtcagcgc cacgccgcga
    13981 gcggagcggt tcagcagcgt tgttgccagc gtattttcaa ggatttggat ccgcttggtg
    14041 acaaatgcag gcgtctgccc aagcgtcgcc gctgcggcgc taaaactccc gctgtggacg
    14101 atttctacca gtacctgcaa gtctttggct aaaggccagc tattcagcat cgttaattat
    14161 ccgcagttgt gataagcgca gtgtatttcg caaaacattg ccattccctg ctctggcgca
    14221 atattcattc ttattttcgc gttgattcac gaattgttaa ttacatattc acggtggcaa
    14281 aaaatataaa accacatttt tagtggtagt ttgtggcggt gaatttttcc agacaaatac
    14341 aaaaactgga gttgccatga tgagcgaaag taataagcaa caggcagtga ataagttgac
    14401 ggagattgtc gctaacttta ccgccatgat ttctacccga atgcctgatg acgtggtgga
    14461 taaactaaaa cagctaaagg atgccgaaac gtcgtcgatg gggaaaatta tctaccatac
    14521 gatgttcgac aacatgcaaa aagcgattga cctgaatcgt cctgcctgtc aggacaccgg
    14581 ggagattatg ttcttcgtta aagtcggttc ccgcttccca ctgcttggcg agctgcaaag
    14641 catactcaaa caagccgtgg aagaggcaac cgtcaaagcg ccactacgtc acaatgcggt
    14701 agaaattttt gacgaagtaa acaccggcaa aaataccggt agcggcgtac cgtgggtcac
    14761 ctgggacatc atccccgaca atgacgatgc ggaaatcgaa gtttacatgg caggcggcgg
    14821 ctgcacgcta cctggccgct cgaaagtgtt aatgccgtca gaaggctacg aaggcgtggt
    14881 gaaattcgtc ttcgaaaata tctccaccct cgccgtaaac gcctgtccac cggtactggt
    14941 gggcgtgggc atcgccacct cggtggaaac cgccgccgta ctctcgcgta aagccatttt
    15001 gcgcccgatt ggcagccgcc atcccaatcc aaaagcggca gaactggagc tacgcctgga
    15061 agaaggactc aaccgtctgg ggattggtcc acaagggctg actggcaaca gttcagtgat
    15121 gggcgtacat atcgaatctg ccgcccgcca tccgtcaacc atcggcgttg ctgtctctac
    15181 cggctgctgg gcgcatcgtc gcggcacact gctggttcat gccgatctca cctttgaaaa
    15241 tctgtctcac acccggagcg cgttatgaaa aagatcctga caaccccgat caaagctgaa
    15301 gatctgcaag atattcgcgt cgacgatgtg atctacctga ccggtacgct ggtgacctgc
    15361 cgtgacgttt gtcaccgccg cttgatcgaa ctgaaacgtc cgatccctta cgatctcaac
    15421 ggcaaagcga ttttccacgc tggccccatc gtgcgcaaaa acggcgataa atgggagatg
    15481 gtttccgttg gcccgacaac cagtatgcgt atggaaagtt ttgaacgtga atttatcgag
    15541 cagaccggcg tgaaactggt ggttggcaaa ggcggaatgg ggccgctgac cgaagaaggc
    15601 tgccagaaat tcaaggcgct acatgtgatt ttcccggcag gctgcgcggt gctggcggca
    15661 acccaggtgg aagagattga agaagtgcac tggacagagc tcggaatgcc ggagtcgctg
    15721 tgggtctgtc gggtcaaaga gttcggcccg ctgattgtct ctattgatac ccacggcaac
    15781 aacctgatag ccgaaaacaa aaagctgttc gccgaacgcc gcgatcccat cgtggaagag
    15841 atctgcgagc acgtccatta catcaaataa ccctcccgga gaggctcgcc ccctctcctt
    15901 tttcgcaggc ataacacgat gaaaccttcc actgaatggt ggcgatacct tgcgccgctg
    15961 gcggtcatcg ccattattgc tctaattccg gttcccgcag ggctggagag tcatacctgg
    16021 ctctactttg ccgtttttac tggcgtgatc gttggactga tcctcgaacc cgtgccgggt
    16081 gccgtggtgg cgatggtggg tatatccatc atcgccatac tctctccctg gctgctgttc
    16141 agcccggagc agctcgctca gccaggcttt aaattcactg caaaatccct ctcgtgggcc
    16201 gtttccggtt tttctaattc ggttatctgg ctgattttcg ccgcctttat gtttggcaca
    16261 ggctatgaaa aaaccgggct tggacgccgc attgcgctga ttctggtgaa aaagatggga
    16321 catcgcacgc tgtttctcgg ctatgcggtg atgttctccg agctgatcct cgcacctgta
    16381 acaccgtcca actcggcgcg tggtgcgggg attatctatc ccatcatccg taacctgcca
    16441 ccgctctatc aatcacaacc aaacgacagc agttcgcgca gcattggctc gtacatcatg
    16501 tggatgggga ttgttgccga ctgcgtgacc agcgccattt tcttgacggc gatggcacca
    16561 aacttgctgt taattggact gatgaaaagc gcatctcacg ccacgctgag ttggggcgac
    16621 tggttcctcg ggatgttgcc gctcagtatt ttgctggtgc tgctggttcc ctggctggct
    16681 tatgtgctgt acccgccggt actgaagtct ggcgatcagg tgccgcgctg ggcagagacg
    16741 gaactgcagg caatgggccc gctctgttcg cgtgaaaaac ggatgctggg gctgatagta
    16801 ggcgcgctgg tgctgtggat tttcggcggt gattatatcg atgccgcgat ggtcggttac
    16861 agcgtggtgg cactgatgct gcttctgcgc attatcagtt gggacgacat tgtcagtaat
    16921 aaagcggcgt ggaacgtttt cttctggctg gcctcgctta tcaccctcgc taccggactc
    16981 aacaacaccg gttttattag ctggtttggc aaactgttag caggcagctt aagcggttat
    17041 tcgccaacga tggtgatggt ggcgttgatt gtggtgtttt atctgctgcg ctactttttc
    17101 gccagcgcca cggcgtatac ctccgccctc gcaccgatga tgattgccgc tgcgctggcg
    17161 atgccggaaa tcccgctgcc ggtgttctgc ctgatggttg gcgcggcaat tggtctgggg
    17221 agcattctta caccatacgc caccggaccc agtccgattt actacggtag tggttatctg
    17281 ccaacggcgg attactggcg actgggggcg atttttgggc tgatattcct cgtgttgctg
    17341 gtgattaccg gcttactgtg gatgcccgtg gtgttgcttt aagtgaggac ggtgccggaa
    17401 attgctctcc ggcacccaat agttttacgc agccggtaac tccgccagcg gccagcgcgg
    17461 acgcacgcta acgccgagat ccgccgtcgc gcctgcttta aaccgcacca ttccggcata
    17521 ggcgatcatc gcgccgttat cagtacaaaa ctccggacgc gcgtagaaca cttcgccgcg
    17581 gcgttttttc atcatttcag ccagcttcgc ccgtaacgtg cggttagcac tcacgccgcc
    17641 cgccatgacc agtcgcttaa agcccgtctg atccaacgct cgcttacact taatcatcaa
    17701 cgtatcgacc accgcatctt caaaggcgcg ggcgatatca gcacgcgtct ggtcgtcggt
    17761 gccgttgtca cgaatcgtgt tcgccgcaaa ggttttcaga ccagaaaagc tgaaatccag
    17821 ccccggacgg tcggtcatcg gacgcgggaa aacaaagcgc ccggcagtac cctgagccgc
    17881 cattttcgac agtaacggtc cgccaggata atccagcccc agcagcttcg cggttttatc
    17941 aaacgcttca ccggcggcat catcgataga ctcgccgagc agctcgtact gaccaatgcc
    18001 agtcacgctg attaactgcg tatgaccgcc ggacaccagc agcgcgacaa acggaaattc
    18061 cggcgggtta tcttccagca tcggcgctaa cagatgccct tccatatggt gtaccgggat
    18121 tgccggaacg ttccaggcaa acgccagaga acgccccacg gttgcgccaa ccagcagtgc
    18181 gccgactaat ccagggcctg cggtataggc cacagcatca atgtcttttg ccgttaagcc
    18241 agactccttt agcgccgcct ggatcaacgg tacggttttg cgcacgtgat cgcgggaggc
    18301 cagttcaggc acgacgccgc catagtcagc gtgcaatttc acctgactat acaattggtt
    18361 ggctaacaaa cctttttcat cgtcgtaaat ggcgatgccg gtttcatcgc aggaagtttc
    18421 aatacccagt acacgcatga cttgttttac ctcgctttat taccgcgcag tgtaggacca
    18481 atgcgggttg atgtaaaact ttgttcgccc ctggagaaag cctcgtgtat actcctcacc
    18541 cttataaaag tccctttcaa aaaggccgcg gtgctttaca aagcagcagc aattgcagta
    18601 aaattccgca ccattttgaa ataagctggc gttgatgcca gcggcaaacc gaattaatca
    18661 aaggtgagag gcacatgccg gtaattaaag tacgtgaaaa cgagccgttc gacgtagctc
    18721 tgcgtcgctt caagcgttcc tgcgaaaaag caggtgttct ggcggaagtt cgtcgtcgtg
    18781 agttctatga aaaaccgact accgaacgta agcgcgctaa agcttctgca gtgaaacgtc
    18841 acgcgaagaa actggctcgc gaaaacgcac gccgcactcg tctgtactaa ttccccgaga
    18901 gcgttgctct ccgatcagac cgagttgtag ttgtaaggcc gtgcttccga aaggaatgcg
    18961 cggcttattt tcgtttatga attgctaaaa atcggggcct atggctggac gaatcccacg
    19021 cgtattcatt aatgatctgc tggcacgcac tgacatcgtc gatctgatcg atgcccgtgt
    19081 gaagctgaaa aagcagggca agaatttcca cgcgtgttgt ccattccaca acgagaaaac
    19141 cccgtcattc accgttaacg gtgagaaaca gttttaccac tgctttggat gtggcgcgca
    19201 cggcaacgcg atcgacttcc tgatgaacta cgacaagctc gagttcgttg aaacggtcga
    19261 agagctggca gcaatgcaca atcttgaagt gccatttgaa gcaggcagcg gccccagcca
    19321 gatcgagcgc catcagaggc aaacgcttta tcagttgatg gacggtctga atacgtttta
    19381 ccaacaatct ttacaacaac ctgttgccac gtctgcgcgc cagtatctgg aaaaacgcgg
    19441 attaagccac gaggttatcg ctcgctttgc gattggtttt gcgccccccg gctgggacaa
    19501 cgtcctgaag cggtttggcg gcaatccaga aaatcgccag tcattgattg atgcggggat
    19561 gttggtcact aacgatcagg gacgcagtta cgatcgtttc cgcgagcggg tgatgttccc
    19621 cattcgcgat aaacgcggtc gggtgattgg ttttggcggg cgcgtgctgg gcaacgatac
    19681 ccccaaatac ctgaactcgc cggaaacaga cattttccat aaaggccgcc agctttacgg
    19741 tctttatgaa gcgcagcagg ataacgctga acccaatcgt ctgcttgtgg tcgaaggcta
    19801 tatggacgtg gtggcgctgg cgcaatacgg cattaattac gccgttgcgt cgttaggtac
    19861 gtcaaccacc gccgatcaca tacaactgtt gttccgcgcg accaacaatg tcatttgctg
    19921 ttatgacggc gaccgtgcag gccgcgatgc cgcatggcga gcgctggaaa cggcgctgcc
    19981 ttacatgaca gacggccgtc agctacgctt tatgtttttg ccggatggcg aagaccctga
    20041 cacgctggta cgaaaagaag gtaaagaagc gtttgaagcg cggatggagc aggcgatgcc
    20101 gctttccgca tttctgttta acagcctgat gccgcaagtt gatctgagta cccctgacgg
    20161 gcgcgcacgt ttgagtacgc tggcactgcc attgatatcg caagtgccgg gcgaaacgct
    20221 gcgaatatat cttcgtcagg aattaggcaa caaattaggc atacttgatg acagccagct
    20281 tgaacgatta atgccaaaag cggcagagag cggcgtttct cgccctgttc cgcagctaaa
    20341 acgcacgacc atgcgtatac ttatagggtt gctggtgcaa aatccagaat tagcgacgtt
    20401 ggtcccgccg cttgagaatc tggatgaaaa taagctccct ggacttggct tattcagaga
    20461 actggtcaac acttgtctct cccagccagg tctgaccacc gggcaacttt tagagcacta
    20521 tcgtggtaca aataatgctg ccacccttga aaaactgtcg atgtgggacg atatagcaga
    20581 taagaatatt gctgagcaaa ccttcaccga ctcactcaac catatgtttg attcgctgct
    20641 tgaactgcgc caggaagagt taatcgctcg tgagcgcacg catggtttaa gcaacgaaga
    20701 acgcctggag ctctggacat taaaccagga gctggcgaaa aagtgattta acggcttaag
    20761 tgccgaagag cgatcgggaa gcccccgaca gccgcactga gaggcagcgg caaatatata
    20821 agtacgccct cgtaattatc gttggcggta aacaaccgtt ggatttcagc gttaacggct
    20881 gaaggacatc gggtcaatcg cccaacacca acctcatgaa ataagtgtgg ataccgtctt
    20941 atggagcaaa acccgcagtc acagctgaaa cttcttgtca cccgtggtaa ggagcaaggc
    21001 tatctgacct atgccgaggt caatgaccat ctgccggaag atatcgtcga ttcagatcag
    21061 atcgaagaca tcatccaaat gatcaacgac atgggcattc aggtgatgga agaagcaccg
    21121 gatgccgatg atctgatgct ggctgaaaac accgcggacg aagatgctgc cgaagccgcc
    21181 gcgcaggtgc tttccagcgt ggaatctgaa atcgggcgca cgactgaccc ggtacgcatg
    21241 tacatgcgtg aaatgggcac cgttgaactg ttgacccgcg aaggcgaaat tgacatcgct
    21301 aagcgtattg aagacgggat caaccaggtt caatgctccg ttgctgaata tccggaagcg
    21361 atcacctatc tgctggaaca gtacgatcgt gttgaagcag aagaagcgcg tctgtccgat
    21421 ctgatcaccg gctttgttga cccgaacgca gaagaagatc tggcacctac cgccactcac
    21481 gtcggttctg agctttccca ggaagatctg gacgatgacg aagatgaaga cgaagaagat
    21541 ggcgatgacg acagcgccga tgatgacaac agcatcgacc cggaactggc tcgcgaaaaa
    21601 tttgcggaac tacgcgctca gtacgttgta acgcgtgaca ccatcaaagc gaaaggtcgc
    21661 agtcacgctg ccgctcagga agagatcctg aaactgtctg aagtattcaa acagttccgc
    21721 ctggtgccga agcagtttga ctacctggtc aacagcatgc gcgtcatgat ggaccgcgtt
    21781 cgtacgcaag aacgtctgat catgaagctc tgcgttgagc agtgcaaaat gccgaagaaa
    21841 aacttcatta ccctgtttac cggcaacgaa accagcgata cctggttcaa cgcggcaatt
    21901 gcgatgaaca agccgtggtc ggaaaaactg cacgatgtct ctgaagaagt gcatcgcgcc
    21961 ctgcagaaac tgcagcagat tgaagaagaa accggcctga ccatcgagca ggttaaagat
    22021 atcaaccgtc gtatgtccat cggtgaagcg aaagcccgcc gtgcgaagaa agagatggtt
    22081 gaagcgaact tacgtctggt tatttctatc gccaagaaat acaccaaccg tggcttgcag
    22141 ttccttgacc tgattcagga aggcaacatc ggtctgatga aagcggttga taaattcgaa
    22201 taccgccgtg gttacaagtt ctccacctac gcaacctggt ggatccgtca ggcgatcacc
    22261 cgttctatcg cggatcaggc gcgcaccatc cgtattccgg tgcatatgat tgagactatc
    22321 aacaaactca accgtatttc tcgccagatg ctgcaagaga tgggccgtga accgacgccg
    22381 gaagaactgg ctgaacgtat gctgatgccg gaagacaaga tccgcaaagt gctgaagatc
    22441 gccaaagagc caatctccat ggaaacgccg atcggtgatg atgaagattc gcatctgggg
    22501 gatttcatcg aggataccac ccttgagctg ccgctggatt ctgcgaccac cgaaagcctg
    22561 cgtgcggcaa cgcacgacgt gctggctggc ctgaccgcgc gtgaagcgaa agttctgcgt
    22621 atgcgtttcg gtatcgatat gaacaccgac cacacgctgg aagaagtggg taaacagttc
    22681 gacgttaccc gcgaacgtat ccgtcagatc gaagcgaagg cgctgcgcaa actgcgtcac
    22741 ccgagccgtt ctgaagtgtt gcgtagcttc ctggacgatt aatcggtagg ccggatcagg
    22801 cgttacgccg cacccggcac tagtccctct gcacaaacgc cacctttt
//
LOCUS       DAZRUF010000044        22728 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZRUF010000044 DAZRUF010000000
VERSION     DAZRUF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.044"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..446)
                     /gene="folK"
                     /locus_tag="ACHXOL_001375"
     CDS             complement(<1..446)
                     /gene="folK"
                     /locus_tag="ACHXOL_001375"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR6808395.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVL"
     gene            complement(443..1840)
                     /gene="pcnB"
                     /locus_tag="ACHXOL_001376"
     CDS             complement(443..1840)
                     /gene="pcnB"
                     /locus_tag="ACHXOL_001376"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR6808396.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1900..2796)
                     /gene="gluQRS"
                     /locus_tag="ACHXOL_001377"
     CDS             complement(1900..2796)
                     /gene="gluQRS"
                     /locus_tag="ACHXOL_001377"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR6808397.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2863..3318)
                     /gene="dksA"
                     /locus_tag="ACHXOL_001378"
     CDS             complement(2863..3318)
                     /gene="dksA"
                     /locus_tag="ACHXOL_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR6808398.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3496..4200)
                     /gene="sfsA"
                     /locus_tag="ACHXOL_001379"
     CDS             complement(3496..4200)
                     /gene="sfsA"
                     /locus_tag="ACHXOL_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR6808399.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4215..4745)
                     /gene="thpR"
                     /locus_tag="ACHXOL_001380"
     CDS             complement(4215..4745)
                     /gene="thpR"
                     /locus_tag="ACHXOL_001380"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR6808400.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4819..7248
                     /gene="hrpB"
                     /locus_tag="ACHXOL_001381"
     CDS             4819..7248
                     /gene="hrpB"
                     /locus_tag="ACHXOL_001381"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR6808401.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7445..9979
                     /gene="mrcB"
                     /locus_tag="ACHXOL_001382"
     CDS             7445..9979
                     /gene="mrcB"
                     /locus_tag="ACHXOL_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR6808402.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10199..12388
                     /gene="fhuA"
                     /locus_tag="ACHXOL_001383"
     CDS             10199..12388
                     /gene="fhuA"
                     /locus_tag="ACHXOL_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR6808403.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12439..13236
                     /gene="fhuC"
                     /locus_tag="ACHXOL_001384"
     CDS             12439..13236
                     /gene="fhuC"
                     /locus_tag="ACHXOL_001384"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR6808404.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13236..14126
                     /gene="fhuD"
                     /locus_tag="ACHXOL_001385"
     CDS             13236..14126
                     /gene="fhuD"
                     /locus_tag="ACHXOL_001385"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR6808405.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMIW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14123..16105
                     /gene="fhuB"
                     /locus_tag="ACHXOL_001386"
     CDS             14123..16105
                     /gene="fhuB"
                     /locus_tag="ACHXOL_001386"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR6808406.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16263..17543)
                     /gene="hemL"
                     /locus_tag="ACHXOL_001387"
     CDS             complement(16263..17543)
                     /gene="hemL"
                     /locus_tag="ACHXOL_001387"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR6808407.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17709..17756
                     /gene="yadX"
                     /locus_tag="ACHXOL_001388"
     CDS             17709..17756
                     /gene="yadX"
                     /locus_tag="ACHXOL_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR6808408.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17768..19189
                     /gene="clcA"
                     /locus_tag="ACHXOL_001389"
     CDS             17768..19189
                     /gene="clcA"
                     /locus_tag="ACHXOL_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR6808409.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19213..19278
                     /gene="yadW"
                     /locus_tag="ACHXOL_001390"
     CDS             19213..19278
                     /gene="yadW"
                     /locus_tag="ACHXOL_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR6808410.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19271..19615
                     /gene="erpA"
                     /locus_tag="ACHXOL_001391"
     CDS             19271..19615
                     /gene="erpA"
                     /locus_tag="ACHXOL_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR6808411.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19662..20285)
                     /locus_tag="ACHXOL_001392"
     CDS             complement(19662..20285)
                     /locus_tag="ACHXOL_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR6808412.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20323..21123)
                     /gene="btuF"
                     /locus_tag="ACHXOL_001393"
     CDS             complement(20323..21123)
                     /gene="btuF"
                     /locus_tag="ACHXOL_001393"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR6808413.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21116..21814)
                     /gene="mtnN"
                     /locus_tag="ACHXOL_001394"
     CDS             complement(21116..21814)
                     /gene="mtnN"
                     /locus_tag="ACHXOL_001394"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR6808414.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21898..>22728
                     /gene="dgt"
                     /locus_tag="ACHXOL_001395"
     CDS             21898..>22728
                     /gene="dgt"
                     /locus_tag="ACHXOL_001395"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR6808415.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     L"
BASE COUNT         5139 a         5793 c         6358 g         5438 t
ORIGIN      
        1 tgtaagactt cacgcaacgt ctccccatca ggaaacgcca gctccggtgc aatctcaaac
       61 agcggccaca gcataaatcc acgattcttc atatcgtagt gcggaacggt caggcgttcg
      121 gtatttatca cttcattacc aaacagcatg atatcgagat ccagcgtgcg tggtccccag
      181 cgttcagctt tacgaacacg accctgttgc agttctatgc gctgtgtatg gttgagtaac
      241 tcttccgggg cgagcgaagt ttcaagcgcc acggcggcgt tgaggtaatc gggttgatct
      301 tgcggcccca gcggtggagt gcggtaaaac gaagaaacgg caagaatgtg gctttcaggg
      361 atatcgccta atgctttcag ggcagcattg acctgctcca gcggagaggc cagattgctg
      421 cctatggcaa tatacgccac tgtcatgcgg taccctcacg acgcggtgcg cgtttgcgtg
      481 gacgacgagt acggcgacgc ggtgacggct cttcatccaa ctcgttaagc atcccttttt
      541 gatctggtgg cgcggaaacc tggaactcac cccaccattt caccagacgc tgcagttcag
      601 cgttacgttc aacttcagct cgcaaggcca acaggtcata agccgcacgg aacttaggat
      661 gctccagcag tttccatgcg cgtttaccct gacgtcggga catacgcaac tgcaactgcc
      721 agatatcgcg ggttaacgtc gtcaggcgtt tcgggattgc cagtgaacgg caggcttcgt
      781 ccagcacgtc gttcatcgcc agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg
      841 cgatcttctg tgccgtctcc agcagtgggt accagaacat ggcggcaaac aagaacgccg
      901 ggttcacgcg catatcgtta tggatacgcg tatcggtatt cttcagcacc tgttcaatga
      961 tccgctccat cgggctgtcg ccattttccg tgaagtagcg ggtaatggtc gggaacagcg
     1021 gctggaacag atgatattca cacaacagct tataggtttc gtaaccgtag cccgcttgta
     1081 gcagtttaag cgattcttca aacaggcgtg ccggtgggat atcgttcagc agggtagcga
     1141 ggcgagggat cggttctgcg gtttccgggc tgatgcgcat acccaatttg gcggcaaaac
     1201 gtaccgcgcg cagcatccgt accggatctt cacggtagcg cgtttccggg ttaccaatca
     1261 gacggataac gccgtccttc agatccttca taccgccaac gtaatcacgg acggtaaaat
     1321 ccgctacgct gtaatagagg ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga
     1381 tggagccgaa aatgttgtcg cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc
     1441 ggtcgctgac gttaccttcg tggtgtccac ggaaggtcgc aacttcgata atctccgggc
     1501 caaacattac atgagccaga cggaaacggc gacccaccag gcggcagtta cggaacagtt
     1561 tgcgcacctg ctcaggcgtg gcgttagtgg ttacgtcaaa atctttcggc tttttgccaa
     1621 gtaacaggtc gcgcacgccg ccgccaacca gccaggcttc gtatcccgct ttgttgagcc
     1681 tgtacattac cttcagggca ttttcactga tatctttgcg ggaaatagca tgctgctcac
     1741 gcgggatcac cgtcacctgt ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc
     1801 ttagcacctt gcggcaaaaa ttagcgactc gggtaaaaat agtacacctc ggtagtgtca
     1861 aacatcattc aggacaaaaa aatagcggct aatcatagct cagcatgacg catttgagaa
     1921 tgttgaattt acaattgccg actcgggcac ggcggtcagc gtccagtttt tgactgctga
     1981 ctgaaggatt tgttcgacgc tgaaatcctg ccagtgtgct tctgcctgct gacccagaaa
     2041 ttgaagtgcc gcgattagta ccggacgtgg atcgcctttc ggcaacgcag gcgcatgatt
     2101 ctgcttggaa agtttagcgc cttgtggatt aagcgccagc ggcagatgaa tgtaatctgg
     2161 cactttccag ccaaaaagct ggtacagcga gatttgcctt actgttggtt caatcagatc
     2221 agccccacgc actatttctg taacgccctg gaaatgatca tcaaccacaa cagccaggtt
     2281 gtaggcgaac aacccatcac ggcgatgaat gataaaatct tcccgtgcca gtttttcgtc
     2341 ggcgtgaata atgccgcgca gctggtcagt aaattgcgtg accggatgct gctggcggat
     2401 acgcactgcg gcattgtctg gtccatgatg caacacccgg caatgaccgt cgtaaatacc
     2461 gccaatgctt tgaatacgcg cacgcgtaca ggtgcagtaa taacttagtc cttgttcatg
     2521 taaccaggcg agtgcttcac gataggcgtc gtgacgttgc gattgccaga gaacatcgcc
     2581 gtcccagtgc agaccgtaat gttccagctg gcgcaggata gtttctgcgg caccgggaac
     2641 ttcacgaggc gggtcgatat cttctatgcg taccagccag cgaccttgcc gggcgcgagc
     2701 ctgcaaatag ctgccgagcg cggcgatcag agagccaaaa tgaagctcgc cggaaggaga
     2761 gggggcgaag cggccaatat actgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg
     2821 gagcatttcc cgcctgtggt aaacgtgatg gaacggctgt aattagccag ccatctgttt
     2881 ttcgcgaatt tcagccagcg ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc
     2941 cagacggcga ataccaattt caacaccgca ggattcgcag tagccgaaat cttcgtcttc
     3001 cacttttttc agcgtcttct cgatcttttt gatcagctta cgctcgcgat cgcggttacg
     3061 cagttcgagg ctgaactctt cttcctgggc tgcacggtct accgggtccg ggaagttggc
     3121 tgcttcatcc tgcatatgtg taacggtgcg atcgacttca tccctgagtt gattacgcca
     3181 tgcttccaga atacgacgga agtgcgccag ctgggcttca ttcatatact cttcgcccgg
     3241 cttctcctga tatggttcca ccccagcgat ggcgagaata ctcagggacg atgttttacg
     3301 gttttgccct tcttgcatgt tgcttctcct taacacgcac tatcgatccc catgttcggg
     3361 ggaaaaatga ggccgctata aatagcagat gcttttccgg atagcaatta tctaaacgta
     3421 acacttgaca actgtgtgag gaaaagcgta tttgcgcacg cgaccagaat gtaaattaac
     3481 cagttactta ctttactaca atgtaaccga cagtgatttt ttaagagcca tgccttcagc
     3541 agaaatttcc gctttgtaag ccagaatttc tacccccctc tgttgagctt ctgacaatag
     3601 ttgcgcgtat ttctcatcga tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag
     3661 cacggcgaaa aagataaccg cacgctggcc ttcagccgct acgttcatca actcccgaag
     3721 gtgtttctga cctcgttcag tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa
     3781 cgtaaccgat ttcacttcaa tatagcagtc tggacgcgaa tccgcctgca acataaagtc
     3841 aatacggctg cgttctgcgc cgtattttac ttcgcttttc agcgagctat agcctgacag
     3901 ttctgaaatt gattcattaa ggatagcctc tttcgtcaac ctgttagccc aaagcgtatt
     3961 gacgcaaata aatgcgccgc tctggctttg agttagttcc caggtgtgtg ggtatttccg
     4021 tttggtgttg tctgaagtcg aataccagac ggtatcgcca cgcgttgcac aaccggtcat
     4081 cgcacccgta ttcgggcagt gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc
     4141 taaaaaacgt ttgtaacgct gaattagcgt cgcgcgctgt agagggggag agaattccat
     4201 tgcgacaatc cttgttattg cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt
     4261 ccacgggcaa acgaggaggc gtaaagggtg aactccgtca ccgcataaga ccagttaaaa
     4321 cctggcggcg ggattgtcac cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga
     4381 aacggacgat tgctttgaaa acaaccgctg cgggcagcct gtgaacggag catattcgcc
     4441 agctggatta agccgcgtgg cggctgacgc atccctaacc acaccacacg cgaacgcagc
     4501 cattgtccgg cgtcatcaag cgtgagtgtg aaaccaggtt gacgaatccg tccggctaaa
     4561 agagaaagcg ccttctcttt ctcggcgctc acttcgccta aaaatgccag agtcagatgc
     4621 aaattatcgg cggcgaccgg acgtcccgcc tcaggtggga agtgtgtggc gcgccagtgg
     4681 ataatctgtt cgcggatttc tgcaggtaag tcgatagcaa agaacagacg ttgcggttca
     4741 gacatgtgag gcactcggtt atgaattacc gcgatgctac aatgtggcgc gaagaatgtt
     4801 aaccctctgg agcgttttgt gtcgtcgttg cccgttgctg ccgtcttacc tgaattactt
     4861 accgctctcg attgtgcgcc gcaggtatta ttaagtgcgc cgaccggggc cgggaaatca
     4921 acctggctgc cgctgcaact gctggcgcat cccggcatta acgggaaaat tatcctgctg
     4981 gagccgcgtc gtctggcggc gcgtaacgtc gcgcaacggc tggcggagct gcttaacgaa
     5041 aagccaggcg ataccgttgg ctaccggatg cgtgcgcaaa actgcgtcgg gccaaatacc
     5101 cgtctggaag tagttaccga aggcgtgctg acgcggatga tccagcgtga cccggagttg
     5161 agcggtgttg gattggtgat ccttgatgaa tttcatgagc gcagcttgca ggcggatttg
     5221 gcgttggcgc tgttactcga tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt
     5281 atgtcggcta cgctggataa cgaccgcttg cagcaaatgc tgccagaagc gcccgtcgtc
     5341 atctcagaag ggcgctcgtt tccggttgaa cgccattatt taccgctgcc cgcgcatcag
     5401 cgttttgacg aagccgttgc ggtagccacc gccgaaatgc tgcgtcagga aagcggatca
     5461 ttactgttat ttttacctgg cgtcggagaa attcagcgtg tgcaggaaca actggcttcg
     5521 cgcatcggca gtgatgtgtt gctctgcccg ctgtatggcg cgttgtcgct gaacgatcag
     5581 cgaaaagcga tcctcccggc accgcaaggg atgcgcaaag tggtgctggc gaccaatatt
     5641 gctgaaacca gtttaaccat tgaaggtatt cgtctggtgg tggattgtgc ccaggagcgt
     5701 gtggcgcgtt ttgagccgcg cacggggctt acgcgactga ttactcaacg cgttagccag
     5761 gcatccatga cgcagcgtgc cgggcgcgcc gggcgtctgg agccgggtat ctgcctgcat
     5821 ttaatcgcca aagaacaagc agaacgcgct gccgcgcaaa gtgaaccgga gatcttacaa
     5881 agcgatcttt ccggtttgct gatggaatta ctgcaatggg gatgcagcga tccggcgcag
     5941 atgagctggc tggatcaacc gccagtagtg aatctaatgg ccgcgaaacg tctgttacaa
     6001 atgctggggg cgctggacgg tgaacggctt agtgcgcaag ggcaaaaaat ggcagcgctg
     6061 ggtaacgatc cgcgtttagc ggcaatgctg gttagcgcga agaacgacga cgaagctgct
     6121 accgcggcaa aaattgccgc cattctcgaa gagccgccac tgatgggcaa tagtgacctg
     6181 ggcgtggcgt tttcgcgcaa tcaaccagcc tggcagcaac gtagtcagca actgttaaaa
     6241 cgcttaaacg tacgcggcgg tgaggcagac agttcgctta tcgcgccgct acttgccggg
     6301 gcgtttgccg atcgcattgc tcatcgccgt gggcaagatg gacgctatca actggcaaac
     6361 ggcatgggag cgatgctcga tgccgacgac gcgctaagcc gccacgaatg gttgatcgca
     6421 ccgttattat tgcagggcag cgcctcgccg gatgcgcgga ttttactggc gctaccggtc
     6481 gatattgatg aattagtaca acgctgtccg cagctggtgc agcaatctga caccgtggaa
     6541 tgggatgacg cgcaaggtac gctgaaagcc tggcgtcggc tacaaatcgg tcagttgacg
     6601 gtgaaagtgc agccgctggc gaaaccgtca gaagacgagt tgcatcaggc gatgcttaat
     6661 ggcatccgtg ataaaggttt aagcgtgctc aactggacgg cggaagcgga acagctacgc
     6721 ttgcgtttgt tatgcgccgc aaagtggttg ccggaatatg actggccagc ggttgatgat
     6781 gaaagtttat tggcagcgct ggaaacgtgg ctgctgccac atatgacagg cgtacattca
     6841 ctacgcggcc tgaaatcact ggacatttat caggcactac gcggattact tgattgggga
     6901 atgcagcaac gtctggatag tgaattgcct gcgcattaca ctgtgccgac gggaagccgg
     6961 atcgccattc gttatcatga agataacccg cccgcactgg cggtgagaat gcaggagatg
     7021 tttggtgagg ccactaatcc gactatcgcc caggggggcg tgccgctggt gctggagttg
     7081 ctttcacccg cccaaaggcc actacaaatc acacgagatt tgagcgcctt ctggaaagga
     7141 gcgtaccgtg aggtgcaaaa agagatgaaa gggcgttatc ccaaacacgt ctggccggac
     7201 gacccggcaa acacggcacc gacgcgacgg acgaaaaagt attcgtaagg ttggttttct
     7261 ccctctccct gtgggagagg gccggggtga gggcatcagc gtgcgcattt ccccctcacc
     7321 ctaaccctct ccctcaaggg agaggggacc gatcgagcac aaattttgag agatatcttc
     7381 ttctgtcttg taacagaaga acagaaaatc gggcttttgc gcctgaatat tgcggagaaa
     7441 aagcatggcc gggaatgacc gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt
     7501 caaacaaaag gtaagccgtc gtcgttacga agatgacgat gattacgacg attatgatga
     7561 ctatgaggat gaagaaccga tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc
     7621 tcgtggcaaa cgcggctggc tatggctgct gttaaaactg gctatcgttt ttgccgtgct
     7681 gatcgccatt tacggcgttt atctcgatca aaaaattcgt agccgtattg atggcaaggt
     7741 ctggcaactg cccgcggcag tttatggccg aatggtcaac cttgaaccag atatgaccat
     7801 cagcaagaac gaaatggtga agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat
     7861 gacccgtcct ggcgaattta ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt
     7921 tgatttcccg gacagcaaag aagggcaggt gcgtgcgcgt ctgacctttg atggcgatca
     7981 tctggcgacg atcgtcaata tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc
     8041 gcgcctgatc accatgatct cttcgccaaa cggtgagcag cgtctgtttg tgccgcgcag
     8101 tggtttcccg gacttgctgg tggatacttt gctggcgaca gaagaccgtc atttctacga
     8161 gcatgatggg atcagtctct actccatcgg acgtgcggtg ctggcaaacc tgaccgccgg
     8221 acgcacggtg cagggagcga gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc
     8281 cagcgagcgt tcttactggc gtaaagcgaa cgaagcttac atggcgctga tcatggacgc
     8341 gcgttacagc aaagaccgta ttcttgagct gtatatgaac gaggtgtatc tcggtcagag
     8401 cggcgacaac gaaatccgcg gcttcccgct tgcgagcttg tattactttg gtcgcccggt
     8461 agaagagctc agcctcgacc agcaggcgct gttagtcggt atggtgaaag gggcgtccat
     8521 ctacaacccg tggcgtaacc cgaaactggc gctggagcga cgtaatctgg tgctgcgttt
     8581 gctacaacaa cagcagatta tcgatggtga actgtatgaa atgctcagcg cgcgtcctct
     8641 gggcgtacag ccgcgcggtg gggtaatctc tccacagcca gcctttatgc aactggtgcg
     8701 tcaggagctg caggcgaaat taggcgataa ggtaaaagat ctctccggcg tgaagatctt
     8761 cactaccttt gactcggtgg cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc
     8821 ggcactgaag aaacagcgta agttgagcga tcttgaaact gcaattgtgg tcgtcgaccg
     8881 ctttagtggt gaagttcgtg cgatggtggg aggttctgag ccgcagtttg cgggctacaa
     8941 ccgtgcgatg caggcgcgtc gttcgattgg ttcccttgca aaaccagcga cttatctgac
     9001 ggctttaagc cagccgaaaa tctatcgcct gaacacgtgg attgccgatg cgccaatcgc
     9061 gctgcgtcag ccgaatggcc aggtctggtc accgcagaac gatgaccgtc gttatagcga
     9121 aagcggcaga gtgatgctgg tggatgcgtt gacccgttcg atgaacgtgc cgacggtaaa
     9181 tctggggatg gcgctggggc tgcctgcagt tacggagacc tggatcaaac tgggcgtgcc
     9241 gaaagatcag ctgcatccgg ttccggcgat gctgctgggg gcgttgaact taacgccaat
     9301 cgaagtggcg caggcattcc agaccatcgc cagcggcggt aaccgtgcac cgctttctgc
     9361 actgcgttcg gtaatcgcgg aagatggcaa ggtgctgtat cagagcttcc cgcaggcaga
     9421 acgcgctgtt ccggcgcagg cggcgtatct gacactatgg accatgcagc aggtggtaca
     9481 acgtggtacg ggtcgtcaac ttggggcgaa atacccgaac ctgcatctgg cagggaaaac
     9541 agggactacc aacaataacg tagatacctg gtttgcgggc attgacggca gcacggtgac
     9601 catcacctgg gtaggccgtg ataacaacca gccgaccaaa ctgtatggtg ccagcggggc
     9661 aatgtctatt tatcagcgtt atctggctaa ccagacgcca acgccgctga atcttgttcc
     9721 gccagaagat attgcagata tgggcgtgga ctacgacggc aactttgttt gcagcggtgg
     9781 catgcgtgtc ttgccggtct ggaccagcga tccgcaatcg ctgtgccagc agagcgagat
     9841 gcagcagcag ccgtcaggca atccgtttga tcagtcttct cagccgcagc aacagccgca
     9901 acagcaacct gctcagcaag agcagaaaga cagcgacggt gtagccggtt ggatcaagga
     9961 tatgtttggt agtaattaac atctaagcgt gaaataccgg atggcgaatt gccatccggt
    10021 aaaataacat cccacctaaa gtattaaccc tttcttttca tctggttgtt tattaaccct
    10081 tcaggaacgc tcagattgcg taccgcttgc gaacccgcca gcgtttcgaa tattatctta
    10141 tctttataat aatcattctc gtttacgtta tcattcactt tacatcagag atataccaat
    10201 ggcgcgttcc aaaactgctc agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc
    10261 cacagcggtt agcggcatgt ctgtttatgc acaggcagcg gttgaaccga aagaagacac
    10321 tatcaccgtt accgctgcac ctgcgccgca agaaagcgca tgggggcctg ctgcaactat
    10381 tgcggcgaag cactctgcta ctgcgactaa aacggataca ccaattgaaa aaacgccgca
    10441 gtctatttcg gttgtgacaa atgaagagat gcagatgcgt caatttcagt ctgtaaaaga
    10501 agcattaggt tatacacctg gcgttacggt tagcagtcgt ggtgcttcta atacatatga
    10561 ttttgttatc attcgtggtt tctcatctgt tggtctgaat caaaataatt accttgatgg
    10621 actaaaactt cagggcaact tttataacga tgctgtgatt gatccttaca tgcttgagag
    10681 ggttgaactg atgcgtgggc cgacgtctgt tctctatgga aaaagtaatc ctggtgggat
    10741 tatttctatg gtgagtaagc gtccgaccac tgaacccctg aaagaaattc agtttaaaat
    10801 ggggacggat aatctgtttc agaccggatt cgatttcagt gatgcactgg atgataacgg
    10861 tgagttctct taccgtttga cgggcctcgc tcgctcaaca aacgaacagc agaaaaactc
    10921 tgaatctcag cgttatacca ttgctccatc attctcatgg cgtccagacg acaaaaccaa
    10981 ttttactttc ctgtcctatt tccagaatga accagaaacg ggttattacg gttggttgcc
    11041 gaaagagggg accgttgagc cattgcctaa tggtaagcgt ctaccgactg acttcaatga
    11101 aggtgcatcg aataatacat actcccgtaa ccagaaaatg gtgggatata gttttgaaca
    11161 tggttttaat gacaccttca ccgtgcgtca gaatctgcgt ttcagtgaaa tgaaaacctc
    11221 acagaaaagt gtttatggca cagggattgc tggtgatggt cataccctaa accgcgggac
    11281 agtggtggat aatgagcgtc tacaaaactt tagcgttgat acccaacttg aaagtaagtt
    11341 tgctacaggt gacgtcgagc atactttgct gacaggggta gacttcatgc ggatgcgtaa
    11401 tgatatcaat gccagctttg gatccgcacc atccatcgat ctttataaca aatatcaccc
    11461 tgaatacttt gcatttggta gcgcagagcc ataccagatg aatgaaagca aacaaacagg
    11521 tatttatgtt caggatcagg cggaatggaa taaatgggta ttcactctgg ggggacgcta
    11581 cgattggtct aagcaagcga ccactgttcg tgaaaactct tatacgccga ctgaaggtta
    11641 tattgagcgc aatgatcatc agttcacctg gcgcggtggt gtaaattact tattcgataa
    11701 tggtatttca ccttacttta gctatagcca gtcctttgaa ccgagtgctt tcgatctgtg
    11761 gagcaacccg cgcatttcct ataagccatc gaaaggtgaa cagtatgaag ctggcgtaaa
    11821 atatgttccg aatgatatgc cggtcgttgt tacgggcgca gtctatcaat tgacgaaaac
    11881 aaataacctg acagcagacc caacaaaccc attagcgcaa gtcccagcag gtgagattcg
    11941 cgctcgtggt gtggaacttg aagcaaaagc ggcgttaaat gccaatatta acttgacggc
    12001 ttcttatacc tacaccgatg cggaatacac caaagacacc aatctcaaag gtaaaactcc
    12061 agaacaagta ccggagcata tggcatctct ctggggggat tataccttca atgaagggcc
    12121 gctttctggt ttaacattgg gaacaggtgg tcgttttatt ggttccagct atggtgatcc
    12181 ggcaaacact tttaaagtgg gtagcgcagc tgtaatggat gctgttgtaa aatatgatct
    12241 ggcacgcttt ggtatggcgg gatccagcct tgctgtgaac gtcaacaatt tgctcgatcg
    12301 tgagtatgtt gccagttgct tccagactta tggctgcttc tggggcgcag aacgtcaggt
    12361 cgttgcaacc gcaaccttcc gtttctaatt tctcttttgg ggcacggatt tccgtgccca
    12421 tttcacaagt tggctgttat gcaggaatac acgaatcatt ccgataccac ttttgcactg
    12481 cgtaatatct cctttcgtgt gcccgggcgc acgcttttgc atccgctgtc gttaaccttt
    12541 cctgccggga aagtgaccgg tctgattggt cacaacggtt ctggtaaatc cactctgctc
    12601 aaaatgcttg gtcgtcatca gcctccgtca gaaggggaga ttcttcttga tgcccagccg
    12661 ctggaaagct ggagcagcaa agcgtttgcc cgcaaagtgg cttatttgcc gcagcagctt
    12721 cctccggcag aagggatgac cgtgcgtgaa ctggtggcga ttggtcgtta cccgtggcat
    12781 ggcgcgctgg ggcgctttgg tgcggcagat cgcgaaaagg tcgaggaagc tatctcgctg
    12841 gttggcttaa aaccgctggc gcatcggctg gtcgatagtc tctctggcgg cgaacgtcag
    12901 cgggcgtgga tcgccatgct ggtggcgcag gatagccgtt gtctgttgct cgacgaaccg
    12961 acttcggcgc tggatatcgc ccaccaggtt gatgtgctgg cgctggtgca ccgtttaagt
    13021 caggaacgtg gcctgacggt cattgccgtg ttgcacgata tcaatatggc ggcacgctac
    13081 tgtgattatc tggtcgccct gcgcagtggt gaaatgattg ctcagggaac gcctgcggaa
    13141 attatgcgcg gcgaaaccct cgaaatgatt tatggcatcc cgatgggtat tttgccgcat
    13201 ccggcgggtg ctgcacctgt gagttttgtt tattgatgag cggcttacct cttatttcgc
    13261 gccgtcgact gttaacggcg atggcgcttt ctccgttgtt atggcagatg aataccgccc
    13321 acgcggcggc tattgacccc aatcgtattg tggcgctgga gtggttgccg gtggaattac
    13381 tgctggcgct cggcatcgtg ccttacggcg tggcggatac catcaactat cgcctgtggg
    13441 tcagcgaacc accattgccg gactcagtga ttgacgtcgg tttgcgtaca gaacctaacc
    13501 ttgaactgct gaccgaaatg aaaccgtcgt ttatgatctg gtcggcagga tatggccctt
    13561 catcagaaat gctggcacgt attgcgccgg gtcgcggatt taacttcagt gacggcaaac
    13621 agccgttggc gatggcgcgt aaatcgctga cggaaatggc agatttactt aacctgcaaa
    13681 gcgcagcgga aacgcattta gcgcaatacg aagactttat ccgcagcatg aaaccccgct
    13741 ttgtgaagcg tggcgcacgc ccgttactgc tgacgacgct tatcgatccg cgccatatgc
    13801 tggtcttcgg tccaaacagc ttgttccagg aaattctcga tgagtacggc atcccaaatg
    13861 cctggcaagg ggaaaccaac ttctggggca gtaccgccgt cagtatcgat cgtctggcag
    13921 cgtataaaga cgttgatgtg ctctgttttg atcacgacaa cagcaaagac atggatgcgc
    13981 taatggcaac cccgctgtgg caggccatgc cgtttgtccg cgccggacgc tttcagcgcg
    14041 tacctgcagt ctggttttat ggtgcgacac tctcggcaat gcactttgtg cgcgttctgg
    14101 ataacgccat cggaggtaaa gcgtgagtaa acgaattgcg ctttttccgg cgttattgct
    14161 ggcgctgtta gtgattgtcg ctacggcgct cacctggatg aacttctcgc aggcgctgcc
    14221 gcgtagccag tgggcgcagg ctgcctggtc gccagacatc gatgtcatcg agcagatgat
    14281 ttttcactac agcttgttgc cgcgtctggc gatttcgctg ctggtgggcg cgggcctggg
    14341 gctggtgggc gtgctgtttc agcaagtgct gcgtaacccg ctggcggagc cgacgacgct
    14401 tggcgttgct acaggcgcgc aactgggaat taccgtcacc acactttggg cgatcccagg
    14461 tgctatggcg agccagtttg ccgcactggt gggcgcttgt gtcgttggct taatcgtctt
    14521 tggcgtcgcg tgggggaaac ggctttcgcc ggttacgctt attctcgcgg ggctggtggt
    14581 gagcctttat tgcggggcaa ttaatcagct actggttatc ttccatcacg accagctgca
    14641 aagcatgttc ctgtggagca ccggaacgct gacgcaaacc gactggggcg gcgttgagcg
    14701 tttatggccg cagttgctgg gcggcgtgat gttgacgtta ttgctacttc gcccgttaac
    14761 cctgatgggg cttgatgatg gcgtggcgcg caatctcggg ctggccttgt cgctcgcgcg
    14821 tctggcggcg ctgtcgctgg cgatagtcat cagtgcgctg ctggtgaatg cggtggggat
    14881 tatcggcttt atcggtttgt tcgcgccgct gctggcgaaa atgctggggg cgcggcgttt
    14941 gttgccacgg ttgatgctgg cgtcgctgat tggggcgctg atcctctggc tttccgatca
    15001 aatcatcctc tggctgactc gcgtgtggat ggaagtgtcc accggttcgg tcactgcgtt
    15061 gatcggtgcg ccgctgctac tgtggctgtt gccgcgttta cgcagcatta gcgcgccgga
    15121 tatgaaggtc aacgatcgtg tcgcggctga acgccaacat gtgctggcgt ttgccctcgc
    15181 gggcggcgtg ctgctgttga tggctgtggt ggtggcgctg tcgtttggtc gtgatgcgca
    15241 cggctggacg tgggcgagcg gggcgttgct cgaggatttg atgccctggc gctggccgcg
    15301 aattatggcg gcgctgtttg cgggcgtcat gctggcggtg gcgggctgta ttattcagcg
    15361 gctgaccgga aacccgatgg caagcccgga agtgctgggg attagctctg gcgcggcgtt
    15421 tggcgtggtg ttaatgctgt ttctggtgcc gggtaatgcc tttggctggc tgttacccgc
    15481 gggcagtatc ggggcggcgg tgacgctgtt gatcattatg atcgccgccg gccgcggtgg
    15541 attttcccca caccgtatgt tactggcggg gatggcgtta agtaccgcat tcaccatgct
    15601 tttgatgatg ttgcaggcga gcggtgaccc gcgaatggcg caagtgctga cctggatttc
    15661 cggttcgacc tacaacgcga ccgatgcgca ggtctggcgt accggaattg tgatggtgat
    15721 tttgctggcg attaccccgc tgtaccgccg ctggctgacc attttaccgc tgggtggtga
    15781 taccgcacga gccgtaggaa tggcgctgac gccgacgcga attgcgctgc tgctgttagc
    15841 ggcttgcctg acggcgaccg cgacgatgac tattggaccg ttgagttttg ttggtttaat
    15901 ggcaccgcat attgcgcgga tgatgggctt tcgacggacg atgccacaca tcgtaatttc
    15961 ggcgctggtg ggtggtttac tgctggtatt cgctgactgg tgtgggcgga tggtgctgtt
    16021 tccattccag atcccggcgg ggctgctgtc gacctttatc ggcgcgccat attttatcta
    16081 tttgctgaga aagcagagcc gttaattttt ccatataacg gccctgtacg cctggacgga
    16141 taaagcgttt acgcgacaac ggatgcccga tgcgacgctg gcgcgtctta tcgggcctac
    16201 aaacggcccc gaatcgtagg tcggataagg cgttcacgcc gcatccgaca aaccatgctg
    16261 gatcacaact tcgcaaacac ccgacgtgca gcatcgatgg tgttattgat atcttccatg
    16321 ctgtgcgcca cggacataaa gcccgcttca aacgctgacg gtgccaggta aacaccttcg
    16381 tccagcatca tatggaagaa acgcttaaag cgttccacgt cacaggccat cacatcctga
    16441 tagcacgtca cggactcggc gtcggtaaag aaaataccga acatgccgcc aacgtggtta
    16501 acgaccagcg gaattccggc ttcttctgcc gcttccagca gaccttctgc cagacgtgtt
    16561 gtcagctcat ccaacgtttc gtgaacgccc ggttgcgcga cttcattcag acaggcgaaa
    16621 ccggctgcca tcgcaattgg gttaccggaa agcgtacccg cctgatagac cggacccgtc
    16681 ggggccagcg catccattac atcacgacga ccaccgaatg cgcctaccgg cattccaccg
    16741 ccgatgattt tgcccaggca ggtgagatcc ggttccacac cgtaataatc ctgtgcgcca
    16801 gccagtgcca cgcggaagcc ggtcattact tcatcgatga tcagcaatgc gccaaattcg
    16861 tcgcacagcg cacgcagacc tggcaggaag tctggcagcg gtggaacaca gttcatattg
    16921 cctgccaccg gctcgatgat aatacaggca atctcttgcg ggtattgctc aaacgcggcg
    16981 cgtacagaag ccagatcgtt ataagtacag gttaaggtat gtttggcgaa atctgccgga
    17041 acgcccggcg agtttggctg gcctaacgtg agtgcgccag aaccggcttt caccagcagg
    17101 cagtcagcgt gaccgtggta acacccttca aatttaatga ttttgtcgcg accggtaaaa
    17161 ccacgggcca gacggatggc gctcatcgtc gcctcggtgc cggagttcac catgcgcacc
    17221 atatccatgg tcgggaccaa ttcggtcacc agttgcgcca ttttcacttc catttcggtt
    17281 ggtgcaccaa agcttaaacc acgctcggcg gcttcaatca cggcattgcg gattgccgga
    17341 tggttatggc ccagcaccat cggcccccag gaaccgacat aatcgatata ggctttgcca
    17401 tcaacatcgt acagataagc gccgtccgct ttttcgataa acagtggagt gccgcccacg
    17461 ccagtaaagg cgcgaacagg ggagttcaca ccgccaggga tcagctcgcg cgctgcgctg
    17521 taaagatttt cagacttact catggagggt tcctgattcg tagaaaaagt gaatggctgc
    17581 tattctatgt tattcataac aagttaaata ctcgtcaaac atcaggctgc ttgtactggt
    17641 gcaatcctga tttcttagag tataaaagtt ttgtgcattt gaaacattac gctttgcaaa
    17701 ggattttcat ggaacgtgcg agtaaaatgc cgtcatctta tttgtatgac caataagtga
    17761 tcattggatg aaaactgata ctccctcttt agaaacaccg caggccgcgc gcctgcgacg
    17821 cagacaactg attcgccaac ttcttgagcg cgataaaacc ccgttagcca ttttgtttat
    17881 ggcggcagtc gtcggcacgc ttgttgggct ggcagcggtt gcttttgaca aaggtgtcgc
    17941 ctggttgcag aaccaacgta tgggggcgct ggtacatact gctgataatt atccgcttct
    18001 gttaaccgtc gcttttctct gttcggcggt gctggcgatg tttggctact ttttggtgcg
    18061 caaatacgcg ccggaagcag gtggttcggg gatcccggaa attgaagggg cgctggaaga
    18121 tcaacgtccc gttcgctggt ggcgtgtatt gccggtgaag ttctttggcg ggctggggac
    18181 actcggcgga ggcatggtgt tggggcgcga agggccaacc gtgcagatcg gcggtaacat
    18241 tggccgtatg gtgcttgata ttttccgcct gaaaggtgac gaagctcgcc atacgctgct
    18301 ggcaaccggt gctgctgcgg ggctggctgc ggcctttaac gcgccgctgg cgggtatttt
    18361 gtttattatc gaagagatgc gtccgcagtt tcgctatacg ttaatttcga taaaagcggt
    18421 atttattggc gtcattatgt cgaccattat gtaccggatt tttaatcatg aagttgcgtt
    18481 gattgacgtc ggtaaacttt ctgatgcgcc gcttaatacg ctgtggcttt atctgatcct
    18541 cggtattatt tttggcattt tcggccctat ttttaataaa tgggtgctgg ggatgcagga
    18601 tttgctgcac cgtgtgcacg gcggcaatat taccaaatgg gtactgatgg gcggtgcgat
    18661 tggcggtctg tgtggattgc tggggtttgt ggcaccagca acgtcgggcg gcggttttaa
    18721 cctgattcct atcgctaccg cggggaattt cagcatggga atgctggtgt ttatcttcgt
    18781 cgctcgggtc attaccacct tactctgctt ctcttccggc gcgccgggcg gtatttttgc
    18841 cccgatgctg gcgctgggta ctgtgctggg aaccgctttc ggaatggttg ccgttgagct
    18901 gtttccgcaa tatcaccttg aggcggggac gtttgctatt gccggaatgg gggcattact
    18961 ggcggcatct attcgcgcgc cgttaacggg gatcattctg gttctggaga tgaccgataa
    19021 ctaccagctc attttgccaa tgattattac cggtcttggc gcaacactat tagcgcaatt
    19081 taccggcggg aaaccgctat actcggcgat tcttgcgcgc acgctggcaa aacaggaagc
    19141 tgagcaactg gcgcgaagca aggccgcatc agccagcgag aatacttgaa cgaaatacca
    19201 gggtattaga taatggcgat tattattggg ttagaatttg cccaattgcc gatgtcgttt
    19261 ggagcaaaat atgagtgatg acgtagcact gccgctggag tttaccgacg cagcagccaa
    19321 caaagttaaa agcctgatcg ctgacgaaga taacccgaat ctgaaattac gcgtgtatat
    19381 cacaggtggc ggttgcagcg gcttccagta tggtttcacc tttgatgatc aggtgaacga
    19441 aggcgatatg accatcgaaa aacagggcgt tggcttggtg gttgatccga tgagcctgca
    19501 atatctggtc ggcggttccg ttgactatac cgaaggactg gaaggttctc gtttcatcgt
    19561 gaccaacccg aacgcgaaaa gcacctgcgg ttgcggttct tcctttagca tctaatctgt
    19621 tgttcatgtg ccggataaac tatccggcgc atcttctctc ttcaacgccc attctcatcc
    19681 agcgcaaacg tcggtagctt aagatgccaa cgaatagccg ccagccgaat caatagcgtc
    19741 acgaccatgc ccatcatact ggctgtttcc agtggtacgg aaaatgtgta ataagccgta
    19801 gcgtggacaa taccgccgat aatacaggca gttgcgtaga tttctgtacg taaaatcatg
    19861 gggatctcgc gggccagtac atcacgaatg atcccgccgc caacgccagt aatgacgacc
    19921 atacaaaccg cgattaacgg accgacttcc gcattaaagg ctttattcac gccaatgccg
    19981 acaaacaccg ccagaccaac ggcgtccagc accggcaaca tccattttgg taagcgtctt
    20041 ggctggcgca ccagcacgat ggtcagcatg ctggttacca ttgcaacgac cagatcggtg
    20101 ggatctttca cccaaaatac cgggccgtga tccagcgcca tgtcgcgaat tgtcccgccg
    20161 cctacagcgg taaccacgcc cagtaccaga acaccaaaag ggtccatacg caattttccg
    20221 gctaacaaaa cgccggagat ggcaaatacc gctgtgccga ctatatccag ccaatagacg
    20281 agcattgttc aatccccact gagcacctaa aaaggtcagg cgctaatcta cctgtgaaag
    20341 cgcattacag agctgttgtg cagcgaggat aatacgtggg cttgcacgtt caaaccagtc
    20401 actcgtgaga ggaataacgg gaattttgag ctgttcaccc cagtattgtt tgattttagg
    20461 aatttggtcc ggtccgcctg taatgacaat cgcctgtggc gagcgtgcta acacctgttc
    20521 gcggctaact tgcggccagg gaacccggct gtctttaaag atgttttctc cgccacaaac
    20581 ttcgagtacc tggttttgaa tcgactcttt tccactggta aatggcggat taatgccgaa
    20641 ttgcagaaaa acacgttttt taggtttatc agcatattgc gctttcaatt gcgcgtactg
    20701 atccagcagg gattgcgcgg cttgttcggc cttatccggt tgcggactcc agggggccaa
    20761 ttgacgtaac gcattggcaa tttgttcaat gcttgtcgca tcgacccaca tcacttttat
    20821 tcccagcgaa gccagctggt caacctgccg ctcggcatta cctccacgcc aggcaatcac
    20881 cagatcgggt ttcagcgcga caatgcgttc cagattcatc ccctgccagg tggaaacctg
    20941 ctcaatcttt tgcgcttgtg gaggatagtc ggaatagctg ctgaccccaa ccggcgtgat
    21001 cccggcggca aaggcaagtt cagtgttggc gggagaaagc gtgatgacgc gcggcgcggc
    21061 gttgagccac agtggcgcaa gaaaagacag ggcgaccagc gccctgaaca gtgacttagc
    21121 catgtgccag tttctgcacc agtgactcaa ccatcaggct ggactgttta gcggcaacag
    21181 ccaggaactc atcgaagcta agatgagact gttgatcggc cacgtcggag atggcgcgta
    21241 cgacaacaaa cgggacgttg aaattgtggc agacatgggc gattgccgtc gcttccatct
    21301 ctacagcaat ggcctgtggg aagttgtggc ggattttcgc cagaccaaca gaaccgttga
    21361 tgaaagcgtc gccgctaaca atcaggccac gtacagcgtt aagattcagt tcggcaatgc
    21421 aggcctcagc ggcagcgatc agtttatcgt cagctttaaa gcctgccgga cagcctggta
    21481 actgaccgta ttcataacca aatgccgtga catccgcgtc gtgataacgc gcttcgtccg
    21541 agacaacgat atcgcccact ttcagcgttg gtgccaggcc accggcagaa ccggtgttaa
    21601 taatcacatc tggcttgcag tgttccaaca gcagagtggc acccagcgcc gcagcgactt
    21661 taccgatgcc cgatttcaga agcgcaacct cggttccatt cagttggccg gtatagattt
    21721 cgcaaccgcc gagactgata gtttgacggt tttcgatttt gtcacgcagc agcgtaactt
    21781 cttcttccat tgcaccaatg atgccgattt tcatagattt actcgcgata agcccgattt
    21841 gaaagcatag tttaccatgc gcttacgggg aagcgtattt ctcacgcggg agaggacatg
    21901 gcacagattg atttccgaaa aaaaataaac tggcatcgtc gttaccgttc accgcagggc
    21961 gttaaaaccg aacatgagat cctgcggatc ttcgagagcg atcgcgggcg tatcatcaac
    22021 tctccggcaa ttcgtcgtct gcaacaaaag acccaggttt ttccactgga gcgcaatgcc
    22081 gccgtgcgca cgcgtcttac ccactcgatg gaagtccagc aggtgggacg ctacatcgcc
    22141 aaagaaattt taagccgtct gaaagagctt aaattgctgg aagcatacgg cctggatgaa
    22201 ctgaccggcc cctttgaaag cattgttgag atgtcatgcc tgatgcacga tatcggcaat
    22261 ccgccgtttg gtcattttgg cgaagcagcg ataaatgact ggtttcgcca acgtttgcac
    22321 ccggaagatg ccgaaagcca gcctctgact gacgatcgct gcagcgtggc ggcactacgt
    22381 ttacgggacg gggaagaaca gcttaacgag ctgcggcgca agattcgtca ggacttatgt
    22441 cattttgagg ggaatgcaca aggcattcgt ctggtgcata cattgatgcg gatgaatctc
    22501 acttgggcac aggttggcgg tattttaaaa tatacccgtc cggcgtggtg gcgtggcgaa
    22561 acgcctgaga cacatcacta tttaatgaaa aagccgggtt attatctttc tgaagaagcc
    22621 tatattgccc ggttgcgtaa agaacttaat ttggcgcttt acagtcgttt tccattaacg
    22681 tggattatgg aagctgccga cgacatctcc tattgtgtgg cagacctt
//
LOCUS       DAZRUF010000045        22295 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZRUF010000045 DAZRUF010000000
VERSION     DAZRUF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.045"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(94..3243)
                     /gene="acrB"
                     /locus_tag="ACHXOL_001396"
     CDS             complement(94..3243)
                     /gene="acrB"
                     /locus_tag="ACHXOL_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR6808416.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3266..4459)
                     /gene="acrA"
                     /locus_tag="ACHXOL_001397"
     CDS             complement(3266..4459)
                     /gene="acrA"
                     /locus_tag="ACHXOL_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR6808417.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4601..5248
                     /gene="acrR"
                     /locus_tag="ACHXOL_001398"
     CDS             4601..5248
                     /gene="acrR"
                     /locus_tag="ACHXOL_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR6808418.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5376..8738
                     /gene="mscK"
                     /locus_tag="ACHXOL_001399"
     CDS             5376..8738
                     /gene="mscK"
                     /locus_tag="ACHXOL_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR6808419.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8799..8897
                     /locus_tag="ACHXOL_001400"
                     /pseudo
     CDS             8799..8897
                     /locus_tag="ACHXOL_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8950..9111)
                     /gene="rsmS"
                     /locus_tag="ACHXOL_001401"
     CDS             complement(8950..9111)
                     /gene="rsmS"
                     /locus_tag="ACHXOL_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR6808420.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9125..9652)
                     /gene="priC"
                     /locus_tag="ACHXOL_001402"
     CDS             complement(9125..9652)
                     /gene="priC"
                     /locus_tag="ACHXOL_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR6808421.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9722..10099
                     /locus_tag="ACHXOL_001403"
     CDS             9722..10099
                     /locus_tag="ACHXOL_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR6808422.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10252..10803
                     /gene="apt"
                     /locus_tag="ACHXOL_001404"
     CDS             10252..10803
                     /gene="apt"
                     /locus_tag="ACHXOL_001404"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR6808423.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10932..12857
                     /gene="dnaX"
                     /locus_tag="ACHXOL_001405"
     CDS             10932..12857
                     /gene="dnaX"
                     /locus_tag="ACHXOL_001405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR6808424.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12190..12254
                     /gene="dnaX"
                     /locus_tag="ACHXOL_001405"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12910..13239
                     /locus_tag="ACHXOL_001406"
     CDS             12910..13239
                     /locus_tag="ACHXOL_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR6808425.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13239..13844
                     /gene="recR"
                     /locus_tag="ACHXOL_001407"
     CDS             13239..13844
                     /gene="recR"
                     /locus_tag="ACHXOL_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR6808426.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13954..15828
                     /gene="htpG"
                     /locus_tag="ACHXOL_001408"
     CDS             13954..15828
                     /gene="htpG"
                     /locus_tag="ACHXOL_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR6808427.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16009..16653
                     /gene="adk"
                     /locus_tag="ACHXOL_001409"
     CDS             16009..16653
                     /gene="adk"
                     /locus_tag="ACHXOL_001409"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR6808428.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            16785..17747
                     /gene="hemH"
                     /locus_tag="ACHXOL_001410"
     CDS             16785..17747
                     /gene="hemH"
                     /locus_tag="ACHXOL_001410"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR6808429.1"
                     /translation="MRQTKIGILLANLGTPDTPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17744..18703)
                     /gene="aes"
                     /locus_tag="ACHXOL_001411"
     CDS             complement(17744..18703)
                     /gene="aes"
                     /locus_tag="ACHXOL_001411"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR6808430.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18855..20159
                     /gene="gsk"
                     /locus_tag="ACHXOL_001412"
     CDS             18855..20159
                     /gene="gsk"
                     /locus_tag="ACHXOL_001412"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR6808431.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20292..21968)
                     /gene="ybaL"
                     /locus_tag="ACHXOL_001413"
     CDS             complement(20292..21968)
                     /gene="ybaL"
                     /locus_tag="ACHXOL_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR6808432.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22206..>22295)
                     /locus_tag="ACHXOL_001414"
     CDS             complement(22206..>22295)
                     /locus_tag="ACHXOL_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808433.1"
                     /translation="SIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         5431 a         5916 c         5849 g         5099 t
ORIGIN      
        1 gagatcctga gttggtggtt caattactcc ttaatgttcg taggttatgc ataaaaaagg
       61 ccgcttgcgc ggccttagtg attacacgtt gtatcaatga tgatcgacag tatggctgtg
      121 ctcgatatct tcattcttgc ggctaaagcg gcggcgaacc accacaaaga ataccggaac
      181 gaagaagatt gccagtaccg ttgcggtcac catcccgccc attacaccgg tacctactgc
      241 gttctgcgcg ccggaaccag caccagtact gataaccagc ggcataacgc cgaggataaa
      301 cgccagcgag gtcatcagga tcggacgtaa acgcatccgc actgcatcaa gcgtcgcttc
      361 aatcagacct ttaccttctt tatccatcaa gtctttggcg aattcgacga taagtatcgc
      421 gttcttcgcc gacaacccaa tggttgtgag caggcctacc tggaagtaaa cgtcattggt
      481 caggccacgg aaggtggcag ccagcaacgc accgataacc cccagcggaa cgaccagcat
      541 aacggagaac ggaatcgacc agctctcgta cagcgccgcc agacacagga atacgacaat
      601 caacgaaatc gcgtacagtg aaggtgcctg gttgccggag agacgttcct gataggacat
      661 ccccgtccag tcatagccaa caccggtagg cagtttgctc gccagttgtt ccatcagctc
      721 cattgcttca ccggtacttt tacccggtgc cgcctggcct aagatttcca tggatggcag
      781 gccgttgtaa cgttccagac gcggcgaacc gtactcccaa cgagaagagg agaacgccga
      841 gaatggcacc atctgaccat cagcagcacg aacataccag tcgccgatat catccggcag
      901 catacggtat ttcgcttctg acatgacgta aactttcttc acacgaccgc ggtcgataaa
      961 gtcgttcaca tagctaccgc cccatgcagc gcccagagag gtgttaatgt cgttgataga
     1021 aacacccagc gcctgcgctt tttcctggtc gatatcaatc ttaaactgcg gggtatcttc
     1081 cagaccgttt ggacgtacgc tggtcagcat atcagggtgc ttcgctgctt ctgcaagcag
     1141 ctggttacgc gcctgagtca gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa
     1201 gtcaaagccg gttgcagtac ccagttccac gattgcgggc aggttaaagg cgaaaaccat
     1261 cgcatctttg atttgcgaga aagcgcgtgt tgcacgcatg gtaatcgctt caactttgtt
     1321 ttcttcgccc ggacgatcgg cccagtcctt caaggaaacg aacgcaatac cggtattctg
     1381 accacgtccc gcaaagccga agccgttaac ggcgaacacc gactcaacgt tgttcttttc
     1441 tttggtcaga tagtaatgcg ttacctcatt gagcactttc tgcgtacgtt cctgcgttgc
     1501 acctgctggc agctgaacca tggtcataaa tacgccctgg tcctcatctg gcaagaagga
     1561 gcttggcaga cgcacgaaca gataggccat gccgaccacg atgatcagat acagcaccag
     1621 gtaacgcccc gtactgcgca gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt
     1681 ctcgaacatg cggttaaacc agccgaagaa gcctttttta ccttccccgt gatcgccttt
     1741 ggcaatcggt ttcagcatgg tggcacaaag agccggagtc aggatcaacg ctaccagtac
     1801 cgacagcgcc attgctgaaa caatggtaat agagaactga cgatagatag caccagtaga
     1861 accgccaaag aaggccatcg gtacgaatac cgccgacagt accatcgcga taccgaccag
     1921 agcgccctga atctgcccca tcgacttacg ggtagcttct tttggcggca aaccttcttc
     1981 cgccataaca cgctccacgt tttctaccac aacgatagcg tcatccacca acaagccgat
     2041 ggcgagcacc atcccgaaca ttgttagcgt gtttatcgag aagccaaagg cggcaaggac
     2101 ggcaaaggtc ccgagcaata ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa
     2161 gttctgcagg aacagataca taaccaggaa cacgaggatg atcgcttcga ccagcgtttt
     2221 aaccacttcg tgaatagaga ttttcacgaa cggcgtggtg tcgtacgggt aaacaatttt
     2281 cagacccgac gggaagaacg gttccatctt cgccagttca gcacggattg ccgcagcggt
     2341 atccagcgcg tttgcaccgg tcgccagctt gatccccaga ccggaagccg gttggccgtt
     2401 aaactctgcg atgatgtcgt agttctcacc acccagctca atcttcgcca catcacgcag
     2461 cagtacgcgg gaaccatcct gattcacttt cagcaggatt ttgccgaact cttcagtaga
     2521 ggtcagacgc gtctgagcaa taatagaggc gttaagctgt tggcctttca ccggcggcgt
     2581 accaccgagc tgacccgccg caacctgggc gttctgcgct ttgatggcgg taatgacatc
     2641 aaccggcgtt agctggaatt tgttcagctc attcgggttc atccagatac gcatcgcgta
     2701 ctgtgaaccg aacaactgaa catcacccac gcccgacgta cggctgatgg catctttcat
     2761 attcgccgct acgtagtcag agatatcctc ctgcgtcatg gtgccatcgg tgttgataac
     2821 gccgacaacc atcaggaagc tgctggatga tttctcaacg ctcacccctt gctgctgaac
     2881 ttcttgcggc agcaacggca tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat
     2941 atccgcatca gtaccagact caaaggtcag ggtgatctgc acggtacccg tggagtcact
     3001 gttagaggac atgtacatca ggttatcgat accgttcata ttctgttcga taacctgtgt
     3061 caccgtgtcc tgcactgttt tcgcatcagc gccggggtag gaggcggaga tcgttactgc
     3121 cggcggtgca atcgtaggat attgcgccac cggcagtttg aggatcgcca gcccccctgc
     3181 caacatgatg ataatggcga tcacccacgc aaaaatcggg cgatcgataa agaaattagg
     3241 catgtcttaa cggctcctgt ttaagttaag acttggactg ttcaggctga gcaccgcttg
     3301 cggcttgctg gttattatca gcggtaactt cttgtgcttt tacctggaca ccaggacgca
     3361 ctttctgcag cccacttatt actacgcgat cgcctgcttt cagaccttct gtcaccagcc
     3421 acttatcgcc aatagcctgg cttgcaacga tcggacgggt ttccactttg tcatccgcgc
     3481 caactaccag tacggtggca tcaccacgcg gcgtacgggt tacgccctgt tgcgggacta
     3541 aaatagcgtt tggattaagc ccttcttcca gacgtgcgcg cacgaacata cccggcagca
     3601 gagtgtgatc cgggttcggg aagatagcgc gtagggtgat agacccagtg gtctgatcaa
     3661 cggtaacgtc agagaattcc agcgtaccgt cctgcgggaa cttaatgccg tcactggtga
     3721 tcagcgacac tttggctttg ccgttctctt gtttcagcgt gccattcgcc agttcctgtt
     3781 tcaggcgcag gaagtcgttg ctggactggg tcacatcaag gtagatcgga tcaagttgct
     3841 gcacggttgc cagcgcagtc gcctgaccgt tctgtaccaa tgcgccttcc gtcacgttcg
     3901 acttaccaat gcgaccgcta atcggagagg tgactttggt gtaagccaga ttgatccgcg
     3961 cagtttcaac ggcagctttc gccgcagtta ccgcagcatt cgcctgttgc gcatcagcca
     4021 gagcctgatc gtactcttgc ttactgatgt actgagtacc gagcagtttc tgataacgat
     4081 tcaccgtcaa ttgcgcgata ttggctgcag cctgggcttt cgccagatca cctttcgcac
     4141 tgtcgtatgt cgcctgatag gtcgcaggat caatctgata gagagagaca ccagcttcga
     4201 tgtcgctacc ttctttgaaa ttacgcttca ggataatccc gctaacttga ggacgaactt
     4261 ctgcgatccg gtaggcactg gtgcgacccg gaagctcggt tgtgatctgc agaggttcag
     4321 ttttgactgt tactacgcca acggcgggca tctgctggcc accttgttgg gcctgtttgt
     4381 cgtcacatcc tgttagggct aagctgcctg agagcatcag aacgaccgcc agaggcgtaa
     4441 accctctgtt tttgttcata tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc
     4501 aaaagttaat aaacccattg ctgcgtttat attatcgtcg tgctatggta catacattca
     4561 caaatgtatg taaatctaac gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca
     4621 agaagcgcaa gaaacgcgcc aacacatcct cgatgtggct ctacgtcttt tctcacagca
     4681 gggggtatca tccacctcgc tgggcgagat tgcaaaagca gctggcgtta cgcgcggtgc
     4741 aatctactgg cattttaaag acaagtcgga tttgttcagt gagatctggg aactgtcaga
     4801 atccaatatt ggtgaactag agcttgagta tcaggcaaaa ttccctggcg atccactctc
     4861 agtattaaga gagatattaa ttcatgttct tgaatccacg gtgacagaag aacgacgtcg
     4921 attattgatg gagattatat tccacaaatg cgaatttgtc ggagaaatgg ctgttgtgca
     4981 acaggcacaa cgtaatctct gtctggaaag ttatgaccgt atagaacaaa cgttaaaaca
     5041 ttgtattgaa gcgaaaatgt tgcctgcgga tttaatgacg cgtcgggcag caattattat
     5101 gcgcggctat atttccggcc tgatggaaaa ctggctcttt gccccgcaat cttttgatct
     5161 taaaaaagaa gcccgcgatt acgtcgccat cttactggag atgtatctcc tgtgccccac
     5221 gcttcgtaat cctgccacta acgaataacc ctgaatctga ctccaggatt tttcctggac
     5281 attttcgtcg ttgctattct ggttcactgc gtcgtgatat tcttgcggtt tgactttttc
     5341 aggtcgttct tcaggttcag aaaccttcat tcatcatgac tatgttccag tattacaaac
     5401 gatcacggca ttttgttttt tcagcattta ttgcttttgt ttttgtcttg ttatgccaga
     5461 acacggcgtt tgcgcgggcg tcatcgaatg gtgatctgcc gacaaaagcg gacctgcagg
     5521 cgcaacttga ctcactaaat aaacaaaaag atctttctgc tcaggacaaa ctggtgcagc
     5581 aggatctgac agatacatta gccaccctcg ataaaatcga tcgcataaaa gaagagacag
     5641 ttcagctacg gcaaaaagtc gctgaagcgc cggaaaaaat gcgccaggcg accgcggcgt
     5701 taacagcact tagcgatgtc gataacgacg aagaaacgcg caaaattctg agtacgcttt
     5761 cgttgcgcca gctggaaact cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa
     5821 acgatctggc gtcttataac agccagctgg tttcgttaca gacgcagccc gaacgcgtgc
     5881 aaaatgcgat gtataacgct tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga
     5941 ctgatgtcgg cgagactgcc ttacgtccca gccagaaagt gttaatgcag gctcagcagg
     6001 cgttgctgaa tgcggagatt gaccagcagc gtaaaagcct ggaagggaac accgtcttgc
     6061 aggatacctt gcaaaagcaa cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc
     6121 agttgcagct gttgcaagaa gcggtaaaca gcaagcgcct gactttaact gaaaaaacgg
     6181 cgcaggaagc cgtctccccg gatgaagccg cgcgtattca ggctaatccg ctggtgaaac
     6241 aggaactgga aattaaccag cagttaagtc agcgtctgat taccgcgact gaaaacggta
     6301 atcagttgat gcagcaaaac attaaagtca aaaactggct ggagcgggcg ctgcaatcgg
     6361 aacgcaatat taaagagcag attgccgtcc tgaagggcag cctgctgttg tctcgtatcc
     6421 tttaccagca acaacaaacg ctgccctcgg cggatgaact ggaaaacatg accaaccgca
     6481 tcgcggattt gcgtctcgaa cagtttgaag ttaaccagca gcgtgatgca ctcttccaga
     6541 gcgatgcgtt cgtcaacaaa ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc
     6601 acgatgcgtt attgcaagtg gttgatatgc gtcgcgaatt gctggatcaa ctcaacaaac
     6661 agttgggtaa ccagctgatg atggccatta acctgcaaat caaccagcag cagttaatga
     6721 gcgtatcgaa aaacctgaaa tccatcctga ctcagcaaat cttttgggtg aacagtaacc
     6781 gtccaatgga ctgggactgg atcaaagcgt tcccgcaaag cctgaaagat gaatttaagt
     6841 cgatgaaaat cacggtgaac tgggaaaaag cctggcctgc cgtttttatc gctttcctcg
     6901 ccggtttgcc gctgctgttg attgccgggc tgatccactg gcgtctgggc tggctgaaag
     6961 cgtatcaaca gaaactggct tccgctgtgg gttccctgcg taacgatagc cagctcaaca
     7021 caccaaaagc gatccttatc gacctgatcc gcgcgctgcc ggtgtgcctg attattctcg
     7081 cggttggcct gattctgttg accatgcagc tcaacatcag cgaactgcta tggtcgttca
     7141 gtaaaaaact ggcgattttc tggctggtgt ttggcctgtg ctggaaggta ctggagaaaa
     7201 atggcgttgc cgtgcgtcac ttcggcatgc cggagcagca gaccagccac tggcgtcggc
     7261 aaattgtccg catcagtctc gcgttgctgc ctatccattt ctggtctgtg gtggcagaac
     7321 tttccccgct gcatctgatg gatgatgtgc tggggcaggc gatgattttc ttcaacctgc
     7381 tgctgattgc cttcctggta tggccaatgt gccgcgaaag ctggcgtgat aaagagtcgc
     7441 acaccatgcg actggtcacc attaccgtgc tgtcgattat cccgattgca ttgatggtgc
     7501 tgactgccac gggttatttc tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg
     7561 tttatctggt gatcatctgg aacttgctct accagacggt attgcgcggc ttaagcgtag
     7621 cggcgcggcg tatcgcctgg cgtcgtgcgc tggcgcgtcg gcagaatctg gtgaaagagg
     7681 gcgcagaagg tgccgaaccg ccggaagaac ccaccattgc actggaacag gttaaccagc
     7741 agacgctgcg tattaccatg ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa
     7801 tttggtccga tttgatcacc gtgttcagct atctcgacag catcacgctc tggcattaca
     7861 acggcactga agctggcgct gcggtggtga aaaacgtcac catgggcagt ctgttgtttg
     7921 cgattatcgc ctcaatggtg gcctgggcgt tgattcgcaa cctgcctggt ttactggaag
     7981 tgctggtgct ctcgcgactg aatatgcgtc agggcgcgtc gtatgccatt actaccatcc
     8041 ttaactacat cattattgcc gttggtgcga tgacggtgtt cggatcgctg ggcgtctctt
     8101 gggataaact ccagtggctg gccgcagcat tatccgtagg tcttggtttt ggtttacaag
     8161 aaattttcgg taacttcgtc tccggtttga tcattctgtt cgaacgtccg gtgcgtattg
     8221 gcgatacggt aaccattggt agcttctcgg ggacggtaag taagatccgt attcgtgcga
     8281 caacgattac cgatttcgat cgcaaagaag tgatcatccc gaacaaagcg tttgttaccg
     8341 agcgtctgat caactggtcg ttgactgaca ctactacgcg tctggtgatc cgtctcggcg
     8401 tggcctatgg ctccgatctg gaaaaagtgc gtaaagtgtt actgaaggcg gcgactgagc
     8461 acccaagggt gatgcacgaa ccaatgccgg aagtcttctt tacggcgttt ggtgccagca
     8521 cgttggatca tgagctgcgt ctgtatgtgc gtgaactgcg tgaccgtagt cgtactgtcg
     8581 atgagctgaa ccgtactatc gatcagctgt gccgtgaaaa cgacatcaac attgccttta
     8641 accagcttga agtgcatctg cacaacgaga agggcgatga ggtgacggaa gtcaaacgcg
     8701 actacaaagg cgatgacccg acgccagcgg tagggtaata accaaagggg gcgacattta
     8761 gttgccccga gattactgac aaagtgcgcg ttgttcatgc cggatgcggc gtgaccgcct
     8821 tatccggcct acgaaaccgc aagaattcaa tgtattgcag gagcggtgta ggcctgataa
     8881 gcgtagcgca tcaggcagtt ttgcgtttgc ccacaatctt aggggacatt tagcggcccc
     8941 atttatttct cacttttccg cctcatcatc gcgcgttaat ttcttttcat aatcgcgttt
     9001 tacaatatcc agcgcgcgca gcacggtgcg ggctgggatc tgattttctt ccagcagcac
     9061 aatcaaatcg acagccagtt tgacatcgtc aggggcattt tcaagtgaca tactctctcc
     9121 attgctagcg ggttaagcgc gctagcctgt tttcgatttt ttccagcgca tggcggcagc
     9181 gtgccaggcg cgcttcatag gcttccactt cacgatgcag cgtttgctgt tccacgagat
     9241 cggtcacccg cgccaggcgg gctctgcgtt cggcaaccat ctcacgtagc cgccgctcaa
     9301 aatcctgatg ctgaatacgt ttacgctgcc agcgggcaat tttcggtggc gcactgtccc
     9361 actcgcgcaa tgaccaggcg gaggcttcac gcgcgatggc ttccagttgt gccgccagat
     9421 gttccgccag ccaggccact tgcggcagct gttgctgctc aaccgcatga caaagcgcag
     9481 ccagattatc gcccgcctcg tcgagacaag cctgtagtgt tgtcgcacga gtctgaaaaa
     9541 gatgcctgtc gaaacgagca cttagcgtgg cgaactgtga caccggggca caacgctgac
     9601 gcagcgtagc gagctgtcct tccagttttt ccagcagcag ggcggttttc acgataagac
     9661 ctcaatgaaa atgataattg ttatgctaaa gtagccactc tgtaagctga caacactcaa
     9721 tatgcaacga atcattttaa tcatcattgg ctggctggcg gtagtgctgg gtacgctggg
     9781 cgtggtatta ccggtattac cgacgacgcc gtttatcctg ctggcggcct ggtgctttgc
     9841 ccgttcttcc ccgcgctttc acgcctggtt gctgtaccgc tcatggtttg gcagctatct
     9901 acgtttctgg cagaaacatc atgcgatgcc gcgcggcgtc aaaccgcggg cgattttgct
     9961 tattttgctc acgtttgcca tttctctgtg gttcgtccag atgccatggg tgcgcatcat
    10021 gttgctggta attctcgcct gtttgctttt ctatatgtgg cgaattccgg tgattgatga
    10081 aaagcaagaa aagcactgaa gcacaacaat cgcagttgca attattgcgt acagccagta
    10141 cattctggcg ttttcgagca caggcgcagg cggtcaaagg ttaaacaact gttacttttg
    10201 atacgtttaa aacgcgccgt gagtaccacc gtaacaagca ggcatacact tatgaccgcg
    10261 actgcacagc agcttgagta tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa
    10321 cccggcattc ttttccgcga tgtcaccagc ttactggaag acccgaaagc ttacgctctc
    10381 agcatcgact tgctggttga gcgttacaaa aatgcgggca ttaccaaagt tgtcggcacc
    10441 gaagcgcgtg gcttcttgtt tggcgctccg gtagctctgg gtctgggcgt tggctttgta
    10501 ccggtccgta aaccgggcaa actgccgcgt gaaaccatca gtgaaactta cgacctggaa
    10561 tacggcaccg atcagctgga gatccacgtt gatgccatca aaccgggcga caaagttctg
    10621 gtggtggacg atctgctggc aaccggcggc actatcgaag cgaccgttaa actgatccgt
    10681 cgtctgggtg gtgaagtggc tgacgctgcg ttcattatca acctgttcga tctcggcggc
    10741 gaacagcgtc tcgaaaaaca gggcattacc agctacagcc ttgtcccgtt cccgggccat
    10801 taattatcgc cagtcttgtg ctgcccacgc tacggacagc acaagatgtg cattcagcct
    10861 cgccgttctg acggggctgt gttagcatta ccccttcgtg aatccacctt ccagcgtttc
    10921 agagcctgcc aatgagttat caggtcttag cccgaaaatg gcgcccacaa acctttgctg
    10981 acgtcgtcgg ccaggaacat gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta
    11041 ttcatcatgc ttatcttttt tccggcaccc gtggcgtcgg aaaaacctct atcgcccgac
    11101 tgctggcgaa ggggctaaac tgcgaaaccg gcattaccgc gacgccgtgc ggcgtgtgcg
    11161 ataactgtcg tgaaatcgag caggggcgct ttgtcgatct gattgaaatc gacgccgcct
    11221 cgcgcaccaa agttgaagat acccgcgacc tgctggataa cgtccagtac gctccggcgc
    11281 gtggtcgttt caaagtttat ctgatcgacg aagtgcatat gctgtcgcgc cacagcttta
    11341 acgcactgtt aaaaaccctt gaagagccgc cggagcacgt taagtttctg ctggcgacga
    11401 ccgatccaca gaaattgccg gtgacgattt tgtcgcgctg tttgcaattt catctcaagg
    11461 cgctggatgt cgagcaaatt cgccatcaac ttgagcacat cctcaacgaa gaacatatcg
    11521 ctcacgagcc gcgggcgttg caattgctgg cgcgcgccgc tgaaggcagc ctgcgagatg
    11581 ccttaagtct gaccgaccag gcgattgcca gcggtgacgg ccaggtttca acccaggcag
    11641 tcagtgcgat gctgggtacg cttgatgacg atcaggcgct gtcgctggtt gaagcgatgg
    11701 tcgaggccaa cggcgagcgc gtaatggcgc tgattaatga agccgctgcc cgtggtatcg
    11761 agtgggaagc gttgctggtg gaaatgctcg gcctgttgca tcgtattgcg atggtacaac
    11821 tttcgcctgc tgcacttggc aacgacatgg ccgccattga gctgcggatg cgtgaactgg
    11881 cgcgcaccat accgccgacg gatattcagc tttactatca gacgctgttg attggtcgca
    11941 aagaattacc gtatgcgccg gaccgccgca tgggcgttga gatgacgctg ctgcgcgcgc
    12001 tggcgttcca tccgcgtatg ccgctgcctg agccagaagt gccacgccag tcctttgcac
    12061 ccgtcgtgcc aacggcagta atgacgccaa cccaggtgcc gccgcaatca gcgccgcagc
    12121 aggcaccgac tgtaccgctc ccggaaacca ccagccaggt gctggcggcg cgccagcagt
    12181 tgcagtgcgt gcagggagca accaaagcaa aaaagagtga atcggcagcc gctacccgcg
    12241 cgcggccggt gaataacgct gcgctggaaa gactggcttc ggtcaccgat cgcgttcagg
    12301 cgcgtccggt gccatcggcg ctggaaaaag cgtcagccaa aaaagaagcg tatcgctgga
    12361 aggcgaccac tccggtgatg cagcaaaaag aagtggtcgc cacgccgaag gcgctgaaaa
    12421 aagcgctgga acatgaaaaa acgccggaac tggcggcgaa gctagcggca gaagccattg
    12481 agcgcgaccc gtgggcggca caggtgagcc aactttcgct accaaaactg gtcgaacagg
    12541 tggcgttaaa tgcctggaaa gaggagagcg acaacgcagt atgtctgcat ttgcgctcct
    12601 ctcagcggca tttgaacaac cgcggtgcac agcaaaaact ggctgaagcg ttgagcatgt
    12661 taaaaggttc aacggttgaa ctgactatcg ttgaagatga taatcccgcg gtgcgtacgc
    12721 cgctggagtg gcgtcaggcg atatacgaag aaaaacttgc gcaggcgcgc gagtccatta
    12781 ttgcggataa taatattcag accctgcgtc ggtttttcga tgcggagctg gatgaagaaa
    12841 gtatccgccc catttgatcg taagcacagc ttacgttcgt catccttaac gtgattgaga
    12901 gagaaaccta tgtttggtaa aggcggtctg ggtaacctga tgaagcaagc ccagcagatg
    12961 caagaaaaaa tgcagaaaat gcaggaagag atcgcgcagc tggaagtcac cggcgaatct
    13021 ggcgcaggtc tggtaaaagt gaccatcaac ggtgcacaca actgccgtcg cgtagagatc
    13081 gacccgagcc tgctggaaga cgacaaagag atgctggaag acctggtggc tgcagcattc
    13141 aacgacgcag cacgtcgtat tgaagaaacg cagaaagaaa aaatggcctc tgtatcctcc
    13201 ggaatgcagc tgccgcctgg ctttaagatg ccgttctgat gcaaaccagc ccgctgttaa
    13261 cacagcttat ggaagcactg cgctgtctgc cgggcgttgg cccgaagtcg gcgcagcgta
    13321 tggcgttcac gctgcttcag cgcgatcgta gcggcgggat gcgtttggcg caggcgctca
    13381 cccgggcgat gtcggaaatc ggccactgcg ccgattgccg cactttcacc gaacaggaag
    13441 tctgtaacat ctgttcgaat ccgcgtcgtc aggaaaacgg tcaaatctgt gtggtggaga
    13501 gtccggcgga catctacgcc attgagcaga cggggcagtt ttctggtcgt tattttgtgt
    13561 tgatggggca tctgtcaccg ctggacggca tcggtccgga tgatatcggg cttgatcgtc
    13621 tggaacagcg tctggcagag gaaaaaatca ctgaagtgat cctcgccacc aatcccacgg
    13681 ttgaaggtga agctaccgct aactacattg ccgagctttg cgcgcaatat gacgtggaag
    13741 ccagccgaat cgctcatggc gtaccggttg gcggcgaact ggaaatggtc gatggcacca
    13801 cgctgtcaca ctcccttgcc gggcgtcata agattcgttt ttaagcaaac gagagcagga
    13861 tcacctgctc tcgcttgaaa ttattctccc ttgtccccat ctctccctca tcctgttttt
    13921 aaccttaaaa tggcattatt gaggtagacc tacatgaaag gacaagaaac tcgtggtttt
    13981 cagtcagaag tgaaacagct tctgcacctg atgatccatt ctctctattc caataaagaa
    14041 atcttcctgc gtgagcttat ctctaacgcc tccgatgcgg cggacaagct gcgtttccgt
    14101 gcgctatcta acccggacct gtacgaaggt gatggcgaac tgcgcgttcg tgtctctttc
    14161 gataaagaca agcgcacgct gaccatctct gataacggcg tggggatgac ccgcgacgaa
    14221 gtgattgacc atctggggac tatcgctaaa tccggtacca aatcattcct cgaatccctg
    14281 ggttctgacc aggcgaaaga cagccagctg atcggtcagt ttggtgttgg tttctactct
    14341 gcgtttatcg tggccgacaa agtgaccgtg cgtactcgcg cggcaggcga aaaaccagaa
    14401 aacggcgtct tctgggaatc ggctggcgaa ggtgaatata ccgttgccga catcaccaaa
    14461 gaagatcgtg gtactgaaat caccctgcat ctgcgtgaag gcgaagacga gttcctcgat
    14521 gactggcgcg tgcgttccat catcagcaaa tactccgacc atatcgcgct gccggtagag
    14581 atcgaaaaac gcgaagagaa agacggcgaa accgttatct cctgggagaa aatcaacaaa
    14641 gcgcaggcgc tgtggactcg taacaagtcg gaaatcaccg atgaagagta caaagagttc
    14701 tacaaacaca tcgcccacga ctttaatgat ccgctgacct ggagccacaa ccgcgttgaa
    14761 ggtaagcagg agtacaccag cctgctgtac attccgtccc aggctccgtg ggatatgtgg
    14821 aaccgtgatc ataaacacgg cctgaaactg tacgtccagc gtgtgttcat catggacgac
    14881 gcagaacagt tcatgccgaa ctatctgcgc ttcgtgcgtg gtctgattga ctccagcgat
    14941 ctgccgctga acgtttcccg tgaaatcctc caggacagca cggtaacgcg taacctgcgc
    15001 aatgcgctga ccaagcgtgt gctgcaaatg ctggaaaaac tggcgaaaga cgacgcggaa
    15061 aaataccaga ccttctggca acagtttggc ctggtactga aagaaggtcc ggcggaagat
    15121 ttcgctaacc aggaagcgat cgccaaactg ctgcgttttg cttctaccca taccgattct
    15181 tctgcgcaga ccgtatctct ggaagactac gtttcccgca tgaaagaagg gcaggagaaa
    15241 atctactaca tcaccgcaga cagctatgcg gcagcgaaga gcagcccgca cctggaactg
    15301 ctgcgtaaga aaggcatcga agttctgctg ctttccgacc gcatcgatga gtggatgatg
    15361 aactatctga ctgagttcga cggtaaaccg ttccagtcgg tgtctaaagt tgacgagtcg
    15421 ctggaaaaac tggctgacga agttgatgag agcgcgaaag aagcggagaa agcactgact
    15481 ccgttcatcg accgtgtgaa agccctgctc ggcgagcgcg tgaaagatgt ccgtctgact
    15541 caccgtctga ccgatacgcc agcgatcgtt tcgaccgacg cggacgaaat gagcactcag
    15601 atggcgaaac tgttcgctgc ggcgggccag aaagtgccag aagtgaaata catcttcgaa
    15661 ctgaacccgg atcacgtact ggtgaaacgt gcggcagata ctgaagatga agcgaagttc
    15721 agcgagtggg tagaactgct gctggatcag gcgctgctgg cagaacgcgg cacgctggaa
    15781 gatccgaacc tgtttattcg tcgtatgaac cagctgctgg tttcctgatg tcatgccgga
    15841 tgacctttgt gtcatccggc atttttcttt tcatcatctg cactttccgc aaattatctc
    15901 gccattaacc gtttcagcct caggtgcctt tcttgaggca atcgcctgtt ggtggtatcg
    15961 tttatcgctt tttcaaaaaa atcgacacat tttaagggga ttttcgcaat gcgtatcatt
    16021 ctgcttggcg ctccgggcgc ggggaaaggg actcaggctc agttcatcat ggagaaatat
    16081 ggtattccgc aaatctccac tggcgatatg ctgcgtgctg cggtcaaatc tggctccgag
    16141 ctgggtaaac aagcaaaaga cattatggat gctggcaaac tggtcaccga cgaactggtg
    16201 atcgcgctgg ttaaagagcg cattgctcag gaagactgcc gtaatggttt cctgttggac
    16261 ggcttcccgc gtaccattcc gcaggcagac gcgatgaaag aagcgggcat caatgttgat
    16321 tacgttctgg aattcgacgt accggacgaa ctgattgttg atcgtatcgt aggccgccgc
    16381 gttcatgcgc cgtctggtcg tgtttatcac gttaaattca atccgccgaa agtagaaggc
    16441 aaagacgacg ttaccggtga agaactgact acccgtaaag acgatcagga agaaaccgtg
    16501 cgtaaacgtc tggttgaata ccatcagatg actgcaccgc tgatcggcta ctacttcaaa
    16561 gaagcggaag cgggtaacac caaatacgcg aaagttgacg gcaccaagtc ggttgctgaa
    16621 gttcgcgctg atctggaaaa aatcctcggc taatcaagcc gcagcagatc tgctttcggg
    16681 caggtctgct catccacctc aggcaatacc tctcacagca attagttctt cttcctcact
    16741 tttccgctac aattatcaac aagttgaatc gataagaggc ggtaatgcgt cagactaaaa
    16801 tcggtatcct gctggcaaac ctgggtacgc ccgatacccc cacacctgaa gcggtaaaac
    16861 gctatctgaa acaattttta agcgacagac gcgtggttga tacctcacgg ttgttatggt
    16921 ggccattgct gcgcggcgtt attttgccgc tgcgctcgcc gcgtgtggcg aagctgtacg
    16981 cctcggtctg gatggaaggt ggctcgccgc tgatggttta cagccgccag caacagcagg
    17041 cgctggcaca acgtttaccg gagacgcccg tagcgctggg aatgagctac ggctcgccat
    17101 cactggaaag cgccgtcgat gaactcctgg cagagcatgt agatcatatt gtggtgctgc
    17161 cgctttatcc gcaattctcc tgttctacgg tcggtgcggt atgggatgaa ctggcacgca
    17221 ttctggcgcg caaacgtagc attccgggga tatcgtttat tcgtgattac gccgataacc
    17281 acgattacat taatgcactg gcgaacagcg tacgcgcttc ttttgccaaa catggcgaac
    17341 cggatctgct gctgctctct tatcatggca ttccccagcg ttatgcagat gaaggcgatg
    17401 attacccgca acgttgccgc acaacgactc gcgaactggc ttccgcactg gggatggcac
    17461 cggaaaaagt gatgatgacc tttcagtcgc gctttggccg ggaaccctgg ctgatgcctt
    17521 ataccgacga aacgctgaaa atgctcggag aaaaaggcgt aggtcatata caggtgatgt
    17581 gcccgggctt tgctgcggat tgtctggaga cgctggaaga gattgccgag caaaaccgtg
    17641 aggtcttcct cggtgcgggc gggaaaaaat atgaatatat tccagcgctt aatgccacgc
    17701 cggaacatat cgaaatgatg gctaatcttg ttgccgcgta tcgctaaagc tgagcggtaa
    17761 agaactgagc gccgtcgcga agagcctcgt cggcggtttt catcatccgt gaataatgca
    17821 aaaaggcatg cagcgtacct gggtagagtt tgaactcaca gggctgctga tgcgccgcta
    17881 acgtctggta aagcagacgg ctgtcatcca gcagcggatc gaactccgcc ccggcaataa
    17941 aacagggcgg aacttcgcga gtgagatcat tattaaacag acagtaatac ggtgactcgc
    18001 ggtccgcgtc gttgcttaaa tacgcctctt catacatctg caaatcctgt tgcgttaagc
    18061 catcccagac accgcccaac agacgacgag tcacggaatc ccgtaatccg taaagcccat
    18121 accacagcaa aacacccgca attttaccgc aatcgatctg tttatcacgc aaccacaacg
    18181 cactggcgag cgccagcatg gcacctgcgg aatcaccggc aaagccaatg cgggacatat
    18241 tgatttgata atcctccgcc tgctggtgga aataacaaca agcagccaca atttcctcta
    18301 tcgcttgcgg aaaacgcgct tcaggtgaaa gggtgtaatc aataccaatc accgtacatt
    18361 ggctgtagct tgccagcagg cgcatgatgc gatcgtgggt atcgagattg ccgagaataa
    18421 aaccgcctcc atgcaaataa aatagcgtcg ccgggctatc tggctgcgga caaaagagac
    18481 gtgtttccac ctgcccatat tttgttggaa ccatgtaagc tctggttgcc atttctggag
    18541 cgcccgcatt ccagaatcgg cgctcaagcg tgtaatactg tcgttgctca gcaatcgttc
    18601 ccgttgcggg ccaggacggt aaatccggct gaagagtatt cacaacggtc ttcatttcag
    18661 cagaaataag gtccagaaca ggtagtttgt tttccggctt catacaaaaa ctcctttcaa
    18721 attacgtcat tgtaaggaac cactgccatg aaaatgcgat cccgcctgct gatattgaaa
    18781 ctggctgcgt ctcgcgcgct cccgtcagat tgtgttaaca ttcgccgctc agttaaccac
    18841 ccgtaaaaac aaccatgaaa tttcccggta aacgtaaatc caaacattac ttccctgtaa
    18901 atgcacgcga tccgctgctt cagcaattcc agccagaaaa cgaaaccagc gccgcctggg
    18961 tcgtaggtat tgaccagacg ctggtcgata ttgaagcgaa agtggatgat gaattcattg
    19021 agcgttatgg attaagcgcc gggcattcac tggtgattga ggatgacgta gccgaagcgc
    19081 tttatcagga gctaaaacag aaaaacctga ttacccatca gtttgcgggt ggcaccattg
    19141 gtaacaccat gcacaactac tcggtgctcg cggacgaccg ttcggtgctg ctgggcgtca
    19201 tgtgcagcaa tattgaaatt ggcagttatg cctatcgtta cctgtgtaac acttccagcc
    19261 gtaccgatct taactatcta caaggcgtgg atggcccgat tggtcgttgc tttacgctga
    19321 ttggcgagtc cggggaacgt acctttgcta tcagtccagg ccacatgaac cagctgcggg
    19381 ctgaaagcat tccggaagat gtgattgccg gagcctcggc actggttctc acctcttatc
    19441 tggtgcgttg caaaccgggt gaacccatgc cggaagcaac catgaaagcc attgagtacg
    19501 cgaagaaata taacgtaccg gtggtgctga cgctgggcac caagtttgtc attgccgaga
    19561 atccgcagtg gtggcagcaa ttcctcaaag atcacgtctc tatccttgcg atgaacgaag
    19621 atgaagccga agcgttgacc ggagaaagcg atccgttgtt ggcatctgac aaggcgctgg
    19681 actgggtaga tctggtgctg tgcaccgccg ggccaatcgg cttgtatatg gcgggcttta
    19741 ccgaagacga agcgaaacgt aaaacccagc atccgctgct gccgggcgct atagcggaat
    19801 tcaaccagta tgagtttagc cgcgccatgc gccacaagga ttgccagaat ccgctgcgtg
    19861 tctattcgca cattgcgccg tacatgggcg ggccggaaaa aatcatgaac actaatggag
    19921 cgggggatgg cgcattggca gcgttgctgc atgacattac cgccaacagc taccatcgta
    19981 gcaacgtacc aaactccagc aaacataaat tcacctggtt aacttattca tcgttagcgc
    20041 aggtgtgtaa atatgctaac cgtgtgagct atcaggtact gaaccagcat tcacctcgtt
    20101 taacgcgcgg cttgccggag cgtgaagaca gcctggaaga gtcttactgg gatcgttaag
    20161 ttatcgtcgg ttcgtaggcc agataaggcg ttcacgccgc atctggcatt tggctctcga
    20221 tgcctgatgc gacgctggcg cgtcttatca tgcctacata tttttcatat tttacatccg
    20281 gcaaccaccg tttaccccgt caccacttca cccgccggtg gcgtttccag cagttccagc
    20341 atagtacggg caatttcacg ttcacccatc actacctgat tcgcgccacg ttcggtgata
    20401 tacgtcactt catcgtcata atgggcgcgg gcgataatct caatatccgg gtttttcgcg
    20461 cgggcagacg ccacaatctc acccgcttca taaccgttgg ggatcgtcag gatcagccat
    20521 tttgcacatt ccagatgcgc cagttgcata atttcttcgt tcgctgcatt gcccaataca
    20581 gcgcggaccc cacgctctcg cagctcatca acacgggttc gtgacgtctc aatcaccacc
    20641 agcggaatat cagaggcgag caatttctcc cccagcaggc tgcctacacg accgtaaccc
    20701 accaatagcg catggttgca aatatccact gggatctgtt tctcttcttc gattgcctct
    20761 tccagcgtct gctcttccag cgtttcggtc ttcgccagat atttctccag tagtgcgaac
    20821 agtaccgggt tgagcataat cgacaggatc gcccctgcca gtaccaggtt ttgtccggcc
    20881 tgcggcagta aattcaatgc cattcccagt cccgccagga taaacgcgaa ctcaccaatc
    20941 tgcgccaggc tggcggcgat ggttaatgcc gtacgttggg agtgaccaaa cagacggaca
    21001 aggaacaggg ccgcgaccga tttaccaaac agaataattg ccagcgtcgc cagcactgcc
    21061 agcggttgct gaatcagaat taacggatca aacaacatcc cgacggagac aaaaaacagc
    21121 accgcaaacg cgtcgcgcaa tggcagcgta tcgtgggcgg cacggtgact cagttcagac
    21181 tcgttcagta ccatcccggc aaagaacgcg ccaagtgcga aggagacgtc aaacaactcc
    21241 accgcaccaa aggcaatccc taacgccagc gccagcaccg acagggtaaa tagctcgcga
    21301 gaaccggttg ccgcgctgcg tgccataatc cacggcacca gacggcgacc taccagcatc
    21361 ataatggcga taaatgcgat cactttgccg atggtgatcc ccatatcgac tgcaagagtg
    21421 gcaaagccca catcgccctg ttccatcatt cctgccactg cgggcagcaa caccagcgtc
    21481 agaaccatta ccaggtcttc cacaatcaac caaccgatgg cgatttgccc acgctgactg
    21541 tcaattaatt gccgttcttc aagtgcgcgc agtaacacca cggtactggc ggtggaaaga
    21601 cataaaccga acacgatacc ggtcattaac gaccagccca gcacggcaga gagcgccata
    21661 cccagcagcg tcgccacggc tatctgggcg atcgcaccgg gaatggcgat ggcctttacc
    21721 gccatcaaat ccttcagcga aaagtgcaaa ccgacgccaa acatcaacag aatgacgccc
    21781 agttcagcca gttccggcgc aagcttggta tcggcaacaa agcccggagt gaatggtcct
    21841 gccagcacac ccgctaacag atatcccacc agaggagaaa tacgtagttt attggccagc
    21901 atgccgagga taaaggcgag cacaaggccg ccaacaatgg tggtgataag cggggtggcg
    21961 tgatgcattc cgtctccttt tcctggtggt tattgtccat ttttggccgg gaaaaccaaa
    22021 attacaggta atagtttatg acaatttcat tgatgatgtt catgaataat tattgaattt
    22081 tgcagaaaaa tggaattagc tgcaaaaaaa gcacggatcg gaaaacagaa ggcggtttaa
    22141 cagaggaaag gcttatggcg actgtaaaac gatgcagcca aagtttgcct ttggctgcaa
    22201 tgaaatcagt ctttatgccg gttatcaggc aggaatatgg tcaacatccc caatagtggc
    22261 aggaaagcac agattttata gactaactcg atgct
//
LOCUS       DAZRUF010000046        22147 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZRUF010000046 DAZRUF010000000
VERSION     DAZRUF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.046"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            183..1991
                     /gene="frdA"
                     /locus_tag="ACHXOL_001415"
     CDS             183..1991
                     /gene="frdA"
                     /locus_tag="ACHXOL_001415"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR6808434.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            1984..2718
                     /gene="frdB"
                     /locus_tag="ACHXOL_001416"
     CDS             1984..2718
                     /gene="frdB"
                     /locus_tag="ACHXOL_001416"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR6808435.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2729..3124
                     /gene="frdC"
                     /locus_tag="ACHXOL_001417"
     CDS             2729..3124
                     /gene="frdC"
                     /locus_tag="ACHXOL_001417"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR6808436.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3135..3494
                     /gene="frdD"
                     /locus_tag="ACHXOL_001418"
     CDS             3135..3494
                     /gene="frdD"
                     /locus_tag="ACHXOL_001418"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR6808437.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            3557..4690
                     /locus_tag="ACHXOL_001419"
     CDS             3557..4690
                     /locus_tag="ACHXOL_001419"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR6808438.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            4779..5312
                     /gene="blc"
                     /locus_tag="ACHXOL_001420"
     CDS             4779..5312
                     /gene="blc"
                     /locus_tag="ACHXOL_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR6808439.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5309..5626)
                     /gene="sugE"
                     /locus_tag="ACHXOL_001421"
     CDS             complement(5309..5626)
                     /gene="sugE"
                     /locus_tag="ACHXOL_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR6808440.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(5801..5947)
                     /gene="ecnB"
                     /locus_tag="ACHXOL_001422"
     CDS             complement(5801..5947)
                     /gene="ecnB"
                     /locus_tag="ACHXOL_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR6808441.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(6058..6183)
                     /gene="ecnA"
                     /locus_tag="ACHXOL_001423"
     CDS             complement(6058..6183)
                     /gene="ecnA"
                     /locus_tag="ACHXOL_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR6808442.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(6235..6801)
                     /gene="efp"
                     /locus_tag="ACHXOL_001424"
     CDS             complement(6235..6801)
                     /gene="efp"
                     /locus_tag="ACHXOL_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR6808443.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            6843..7871
                     /gene="epmB"
                     /locus_tag="ACHXOL_001425"
     CDS             6843..7871
                     /gene="epmB"
                     /locus_tag="ACHXOL_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR6808444.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            8261..9130
                     /locus_tag="ACHXOL_001426"
     CDS             8261..9130
                     /locus_tag="ACHXOL_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR6808445.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(9333..9686)
                     /locus_tag="ACHXOL_001427"
     CDS             complement(9333..9686)
                     /locus_tag="ACHXOL_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR6808446.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(9824..11470)
                     /gene="groL"
                     /locus_tag="ACHXOL_001428"
     CDS             complement(9824..11470)
                     /gene="groL"
                     /locus_tag="ACHXOL_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR6808447.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(11514..11807)
                     /locus_tag="ACHXOL_001429"
     CDS             complement(11514..11807)
                     /locus_tag="ACHXOL_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR6808448.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            12083..13339
                     /gene="yjeH"
                     /locus_tag="ACHXOL_001430"
     CDS             12083..13339
                     /gene="yjeH"
                     /locus_tag="ACHXOL_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR6808449.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(13361..13831)
                     /locus_tag="ACHXOL_001431"
     CDS             complement(13361..13831)
                     /locus_tag="ACHXOL_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR6808450.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            14168..15604
                     /gene="aspA"
                     /locus_tag="ACHXOL_001432"
     CDS             14168..15604
                     /gene="aspA"
                     /locus_tag="ACHXOL_001432"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR6808451.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            15722..17023
                     /gene="dcuA"
                     /locus_tag="ACHXOL_001433"
     CDS             15722..17023
                     /gene="dcuA"
                     /locus_tag="ACHXOL_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR6808452.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            17139..17477
                     /gene="cutA"
                     /locus_tag="ACHXOL_001434"
     CDS             17139..17477
                     /gene="cutA"
                     /locus_tag="ACHXOL_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR6808453.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            17453..19150
                     /gene="dsbD"
                     /locus_tag="ACHXOL_001435"
     CDS             17453..19150
                     /gene="dsbD"
                     /locus_tag="ACHXOL_001435"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR6808454.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            19187..19762
                     /locus_tag="ACHXOL_001436"
     CDS             19187..19762
                     /locus_tag="ACHXOL_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR6808455.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            19869..19944
                     /locus_tag="ACHXOL_001437"
     tRNA            19869..19944
                     /locus_tag="ACHXOL_001437"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19902..19904,aa:Phe,seq:gaa)
     gene            20187..20301
                     /locus_tag="ACHXOL_001438"
                     /pseudo
     CDS             20187..20301
                     /locus_tag="ACHXOL_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            20561..20860
                     /locus_tag="ACHXOL_001439"
     CDS             20561..20860
                     /locus_tag="ACHXOL_001439"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR6808456.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(20851..20976)
                     /gene="laoB"
                     /locus_tag="ACHXOL_001440"
     CDS             complement(20851..20976)
                     /gene="laoB"
                     /locus_tag="ACHXOL_001440"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR6808457.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            21043..22098
                     /gene="cadC"
                     /locus_tag="ACHXOL_001441"
     CDS             21043..22098
                     /gene="cadC"
                     /locus_tag="ACHXOL_001441"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR6808458.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
BASE COUNT         5506 a         5618 c         5479 g         5544 t
ORIGIN      
        1 tcagtgataa tttagccctc ttgcgcacta aaaaaatcga tctcgtcaaa tttcagactt
       61 atccatcaga ctatactgtt gtacctataa aggagcagtg gaatagcgtt cgcagaccgt
      121 aactttcagg cacttaccct gaagtacggg gctgtgggat aaaaacaatc tggaggaatg
      181 tcgtgcaaac ctttcaagcc gatcttgcca ttgtaggcgc cggtggcgcg ggattacgtg
      241 ctgcaattgc tgccgcgcag gcaaatccaa atgcaaaaat cgcactaatc tcaaaagtat
      301 acccgatgcg tagccatacc gttgctgcag aagggggctc cgccgctgtc gcgcaggatc
      361 atgacagctt cgaatatcac tttcacgata cagtagcggg tggcgactgg ttgtgtgagc
      421 aggatgtcgt ggattatttc gtccaccact gcccaaccga aatgacccaa ctggaactgt
      481 gggggtgccc atggagccgt cgcccggatg gtagcgtcaa cgtacgtcgc ttcggcggca
      541 tgaaaatcga gcgcacctgg ttcgccgccg ataagaccgg cttccatatg ctgcacacgc
      601 tgttccagac ctctctgcaa ttcccgcaga tccagcgttt tgacgaacat ttcgtgctgg
      661 atattctggt tgatgatggt catgttcgcg gcctggtagc aatgaacatg atggaaggca
      721 cgctggtgca gatccgtgct aacgcggtcg ttatggctac tggcggtgcg ggtcgcgttt
      781 atcgttacaa caccaacggc ggcatcgtta ccggtgacgg tatgggtatg gcgctaagcc
      841 acggcgttcc gctgcgtgac atggaattcg ttcagtatca cccaaccggt ctgccaggtt
      901 ccggtatcct gatgaccgaa ggctgccgtg gtgaaggcgg tattctggtc aacaaaaatg
      961 gctaccgtta tctgcaagat tacggcatgg gcccggaaac tccgctgggc gagccgaaaa
     1021 acaaatatat ggaactgggt ccacgcgaca aagtttctca ggccttctgg cacgaatggc
     1081 gtaaaggcaa caccatctcc acgccgcgtg gcgatgtggt ttatctcgac ttgcgtcacc
     1141 tcggcgagaa aaaactgcat gaacgtctgc cgttcatctg cgaactggcg aaagcgtacg
     1201 ttggcgtcga tccggttaaa gaaccgattc cggtacgtcc gaccgcacac tacaccatgg
     1261 gcggtatcga aaccgatcag aactgtgaaa cccgcattaa aggtctgttc gccgtgggtg
     1321 aatgttcctc tgttggtctg cacggtgcaa accgtctggg ctccaactcc ctggcggaac
     1381 tggtggtctt cggccgtctg gccggtgaac aagcgacaga gcgtgcagca actgccggta
     1441 atggcaacga agcggcaatt gaagcgcagg cagctggcgt tgaacaacgt ctgaaagatc
     1501 tggttaacca ggatggcggc gaaaactggg cgaagatccg cgacgaaatg ggcctggcaa
     1561 tggaagaagg ttgcggtatc taccgtacgc cggaactgat gcagaaaacc atcgacaagc
     1621 tggcagagct gcaggaacgc ttcaagcgcg tgcgcatcac cgacacctcc agcgtgttca
     1681 acaccgacct gctctacacc attgaactgg gccacggtct gaacgttgct gaatgtatgg
     1741 cgcactccgc aatggcacgt aaagagtccc gcggcgcaca ccagcgtctg gacgaaggtt
     1801 gcaccgagcg tgacgacgtc aacttcctca aacacaccct cgccttccgc gatgctgatg
     1861 gcacgactcg cctagagtac agcgacgtga agattactac gctgccgcca gctaaacgcg
     1921 tttacggtgg cgaagcggat gcagccgata aggcggaagc agccaataag aaggagaagg
     1981 cgaatggctg agatgaaaaa cctgaaaatt gaggtggtgc gctataaccc ggaagtcgat
     2041 accgcaccgc atagcgcatt ctatgaagtg ccttatgacg caactacctc attactggat
     2101 gcgctgggct acatcaaaga caacctggca ccggacctga gttaccgctg gtcctgccgt
     2161 atggcgattt gtggttcctg cggcatgatg gttaacaacg tgccaaaact ggcatgtaaa
     2221 accttcctgc gtgattacac cgacggtatg aaggttgaag cgttagctaa cttcccgatt
     2281 gaacgcgatc tggtggtcga tatgacccac ttcatcgaaa gtctggaagc gatcaaaccg
     2341 tacatcatcg gcaactcccg caccgcggat cagggtacta acatccagac cccggcgcag
     2401 atggcaaagt atcaccagtt ctccggttgc atcaactgtg gtctgtgcta cgccgcgtgc
     2461 ccgcagtttg gcctgaaccc agagttcatc ggtccggctg ccattacgct ggcgcatcgt
     2521 tataacgaag atagccgcga ccacggtaag aaggagcgta tggcgcagtt gaacagccag
     2581 aacggcgtat ggagctgtac tttcgtgggc tattgctccg aagtctgccc gaaacacgtc
     2641 gatccggctg cggccattca gcagggcaaa gtagaaagtt cgaaagactt tcttatcgcg
     2701 accctgaaac cacgctaagg agtgcaacat gacgactaaa cgtaaaccgt atgtacggcc
     2761 aatgacgtcc acctggtgga aaaaattgcc gttttatcgc ttttacatgc tgcgcgaagg
     2821 cacggcggtt ccggctgtgt ggttcagcat tgaactgatt ttcgggctgt ttgccctgaa
     2881 aaatggcccg gaagcctggg cgggattcgt cgacttttta caaaacccgg ttatcgtgat
     2941 cattaacctg atcactctgg cggcagctct gctgcacacc aaaacctggt ttgaactggc
     3001 accgaaagcg gccaatatca ttgtaaaaga cgaaaaaatg ggaccagagc caattatcaa
     3061 aagtctctgg gcggtaactg tggttgccac catcgtaatc ctgtttgttg ccctgtactg
     3121 gtaaggagcc tgagatgatt aatccaaatc caaagcgttc tgacgaaccg gtattctggg
     3181 gcctcttcgg ggccggtggt atgtggagcg caattattgc gccggtgatg atcctgctgg
     3241 tgggtattct gctgccactg gggctgtttc cgggtgatgc gctgagctac gagcgcgttc
     3301 tggcgttcgc gcagagcttc attggtcgcg tattcctgtt cctgatgatc gttctgccgc
     3361 tgtggtgtgg tttacaccgt atgcaccacg cgatgcacga tctgaaaatt cacgtgcctg
     3421 cgggcaaatg ggttttctac ggtctggctg ctatcctgac agttgtcacg ctgattggta
     3481 tcgttacaat ctaacgtatc gccaatgtaa atccggcccg cctatggcgg gccgttttgt
     3541 atggaaacca gaccttatgt tcaaaacgac gctctgcgcc ttattaatta ccgcctcttg
     3601 ctccacattt gctgcccctc aacaaatcaa cgatattgtg catcgcacaa ttaccccgct
     3661 tatagagcaa caaaagatcc ccggtatggc ggtggcggta atttatcagg gtaaacctta
     3721 ttactttacc tggggctatg cggacatcgc caaaaagcag cccgtcacac agcaaacgtt
     3781 gtttgagtta ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg acgctattgc
     3841 tcgaggggaa atcaagttaa gcgatcccgc aacaaaatac tggcctgaac ttactgctaa
     3901 acagtggaat gggatcacac tattacatct tgcgacctac accgctggcg gcctgccatt
     3961 gcaggtgccg gatgaagtga aatcctcaag cgacttgctg cgcttctatc aaaactggca
     4021 gcctgcatgg gctccaggaa cacaacgtct gtatgccaac tccagtatcg gtttgttcgg
     4081 cgcactggct gtgaagccgt ctggtttgag ttttgagcag gcgatgcaaa ctcgtgtctt
     4141 ccagccactc aaactcaacc atacgtggat taatgtacct cccccagaag aaaagaatta
     4201 cgcctgggga tatcgcgaag gtaaggcagt gcatgtttcg ccaggggcgt tagatgctga
     4261 agcttatggt gtgaagtcga ccattgaaga tatggcccgc tgggtgcaaa gcaatttaac
     4321 cccccttgat atcaatgaga aaacgcttca acaagggata caactggcac aatctcgcta
     4381 ctggcaaacc ggcgatatgt atcagggcct gggctgggaa atgctggact ggccggtcaa
     4441 tcctgacagc atcattaacg gcagtgacaa taaaattgca ctggcagcac gcccggtaaa
     4501 agcgattaag cccccaactc ctgcagtacg cgcatcatgg gtacataaaa cgggggcgac
     4561 cggcggattt ggtagctatg tcgcgtttat tccagaaaaa gagctgggta tcgtgatgct
     4621 ggctaacaaa aactatccca atccagcgag agtcgccgcc gcctgtcaga ttctcaacgc
     4681 tctacagtaa aatttcatcg ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg
     4741 tcatctacac ttagaaaaaa accagtaagg aaacatttat gcgcctgctc cctctcgttg
     4801 ccgcagcgac agctgcattt ctggtcgttg cctgcagttc tcctacgccg ccgcgtggcg
     4861 tgacagtagt gaataatttc gacgctaaac gctatctcgg tacctggtat gagattgccc
     4921 gttttgatca ccgttttgaa cgtggactgg aaaaagtcac cgcaacatac agtctgcgtg
     4981 atgacggcgg cctgaatgtc atcaataaag gctataaccc tgacagagaa atgtggcagc
     5041 agagtgaagg gaaagcgtac tttaccggcg caccaacacg cgctgcgctg aaagtgtcat
     5101 tctttggtcc tttctatggc ggttataacg tcattgcact tgatcgggaa taccgccatg
     5161 cgctggtttg cggcccggac cgcgactacc tgtggatact ctcccgcacg ccaaccattt
     5221 ctgacgaagt gaaacaggag atgctggcag tcgcgacccg ggaagggttt gatgtcagta
     5281 agtttatttg ggtacagcag cccggtagtt agtgagtgct gagtttcaga ccaataatcc
     5341 ccaatacgat taacgccaga ctcgccaggc gcatcgggtt agcggactca ccgagcagca
     5401 caatgccggt gatggccgcg ccgactgcgc caatacccgt ccacacggca taagccgtcc
     5461 ctaccggtaa cgatttcatc gcccaggcaa gtagcgccat actgactatc atcgccgtca
     5521 cagtaataac acttggcgtc aaacgactaa agccgtgggt atatttcagg ccaacggccc
     5581 ataccacttc cagcagacca gcaataacta agataatcca ggacatatca ggctccggag
     5641 aattggggcc gtccccggtg aaaagaggcg tttgcaggtc gtcctgcaaa gctgatggtt
     5701 gaacggatat tttgccaggt ggcaggggaa tttcaactat tacctttgtg actatcgcaa
     5761 aaaaacaaac ggcacgacat aactgtcgta ccgttattgc ttattgctgc gctttcgttg
     5821 ctgcaccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca cgcgtggtgt
     5881 tgcaggcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca attgtcttct
     5941 tcaccataac gtcttccttt taggtaatgt tatttatgtt tgcctatagc aaattaataa
     6001 tagacaacat cgccaaaaat gatggaaatc tgatgatttt tcggaagaga agaaaaatta
     6061 gctggcagcg cgggagatgg agttgccgag atgtttgatg tcttcgccga aaccgcgagc
     6121 ggtgttacag cccgtgagca gcgtgctggc aagcaaaaca agaacgataa ggcgtttcat
     6181 catctctgac ctgaataagt gatggtgcag cctgcaggcc gcaccacaac cgcattactt
     6241 cacgcgagag acgtattcac cagagcgggt atccactttg atgacttcgc cgatttgtac
     6301 gaacagcgga actttaacca cagcgccagt agacagggta gccggtttgc cgccagtacc
     6361 tgcggtatca cctttcaggc ccggatcggt atcaacgatt tccagttcaa cgaagttcgg
     6421 cggagtaacg gagatcggct gaccattcca cagagttacg atacactctg cctgatccag
     6481 cagccattta gcgttgtcgc caattgcttt tgcatcagca gacagctgct cgaaagtttc
     6541 gttgttcatg aagtgccaga actcaccgtc gttgtacagg taagtcaggt tcatatcgac
     6601 aacatcagcg ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc gagtaccggt
     6661 cagcagacga cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt ttacgaattc
     6721 actcgcttca accgcgtaag gttcgccgtc taacatgatt ttaagaccag cacgaaaatc
     6781 gttgctatag tacgttgcca taaggccctc tgaaatttgt taattggtag ctaagccaca
     6841 aaatggcgca tattgtaacc ctaaataccc catccagaga agattggtta acgcaacttg
     6901 ccgatgttgt gaccgatcct ggtgaacttc tgcgtctttt gaatatagac gcggacgaaa
     6961 aactgttggc cggacgcagc gccaaaaagc tgtttgccct gcgtgtgccc cgctcattta
     7021 tcgatcgcat ggagaaaggc aatccggacg atcctatttt gcgtcaggta cttacctcgc
     7081 aagatgagtt tgtcgtcgcg tccggattct ccaccgaccc actggaagaa cagcacagcg
     7141 tagtgcctgg tttgttgcat aaataccaca accgggcgct tttgctggtc aaaggcggct
     7201 gcgcggtaaa ttgccgctat tgcttccgtc gccacttccc ctatgccgaa aatcagggca
     7261 acaagcgtaa ctggcagact gcacttgagt atgttgctgc gcatccggaa ctggacgaga
     7321 tgattttctc cggcggcgat ccgctgatgg cgaaagatca cgagctggac tggttgctca
     7381 cacaactgga agccatcccg catatcaaac gtctgcggat tcacagccgt ctgccgattg
     7441 tgatcccggc acgtatcacc gaggcgctgg ttgaacgctt tgcccgttct acgctgcaaa
     7501 tcttgctggt gaatcacatc aatcatgcca atgaggtaga tgaaacgttc cgtcaggcga
     7561 tggctaaatt gcgccgtgtc ggtgtcaccc tgcttaacca gagcgttctg ttacgtggtg
     7621 tgaacgataa cgcacaaacg ttggcaaacc tgagtaatgc gttgttcgat gccggcgtga
     7681 tgccgtatta cctgcatgtg ctagataaag tacagggcgc ggcgcatttt atggtgagtg
     7741 atgacgaagc acggcagatt atgcgtgagt tgctgacatt ggtgtcggga tatctggtgc
     7801 cgaaactggc gcgagaaatt ggcggcgaac ccagcaaaac gccgctggat ctccagctac
     7861 gccagcagta actgattaaa cagggaagtt aaacattagc ctgatgcgct acgcttatca
     7921 ggcctacatt gtgcattgaa attgaatgga tttgcaatga actgtaggcc ggataaggcg
     7981 tttacgccgc atccggcaag agcaacaaac gcaagtacag caacctgtca ttaaactggg
     8041 aaatttaatc tttgcccgtt gcataacctt taattattca tatacattgc tgttgttact
     8101 ctttatatag ccaacaccaa taattaacct ttcttttctg tttatataga gtgagtacgg
     8161 ctatttatgt tattttaaca actattacta ccaagcccat ttatctggct tataaattaa
     8221 aattaattat ttaattgaga aatggttagg gagaacctac atggccataa gcatcaaggg
     8281 tgttaatacc ggtgttattc gcaagagtaa taatttcatt gcactggcgc tgaaaattaa
     8341 agaacctcgc aataaagaat cgttgttctt tatgtctgtc atggaactgc gcgacctgct
     8401 gattgcgctg gaaagtcgtc tgcatcaaaa gcataaactg aatgctgctg cacgccttca
     8461 gtatgagcaa gctcgcgata aagtcattaa gaaaatggca gaaaacatcc cagaaattct
     8521 ggttgatgag cttaaaaatg ccgatattaa tcgtcgggtt aacacgctgg aacttaccga
     8581 taatcaaggc gaaaatctta ccttcgtatt aacgctacat gacggtagca aatgtgagtt
     8641 agtcgtcaat gagttgcaga ttgaaatgct ggcgcgagca atcattcatg caattaataa
     8701 tgcggaaatg cgtgaactgg cattacgtat tacctcactg ttagatttcc tgccgttata
     8761 cgacgttgac tgccaggaca atggcaatct ggaatatgac acctattccc aaccggaatg
     8821 gaaacataac ctgttcgacc attatctggc ggtgctttac cgctttaaag atgaaagtgg
     8881 caaagaacaa tttagcggca cagtggtgaa aacgcgtgaa gcaacgccgg gtaaagaaat
     8941 agaggcgatc actcgccgaa tgcttgattt cagcccacga ctgaaaaaac tcgcgggtgt
     9001 gccatgtcag gtctatgttc gcaccgtagc ggcgaataac gcccaaccgc taacccagga
     9061 tcaatgctta cgcgcgctgc atcacttacg tgttcaatcc accagcaaaa ctgcaccaca
     9121 ggcgaaataa tctcatcttt gctaatgcga ggctggagcc atccagcctg aaatgactga
     9181 taacgtgatc gttggtcatg ccggatgcga cgtaaacgcc ttatccggcc tacaaattct
     9241 caagcattca atcagttgca atgcctgctg taggcctgat aagcgaagcg catcaggcaa
     9301 taatacgttt gtcacccagc ctctggatca catcagttcg ggcacttata aacctgaccg
     9361 ataatctggc tatccgtcgg gacaaaactg gacaacattc cctgcgacgg gctgctgatg
     9421 ccataaatca cgttaccgcc cattgcagcc gcctggttgc gcagatcgtt tgctgcgccg
     9481 cgcatagaac cgccctcttc tccgtgttgc ccggaaagcc agttgctttg cttacctgtc
     9541 gcagtaccaa tcagctggca ctctgcgcct ggctgctcgt ccacaatgcg tacactctga
     9601 ccggcagcac tcaattcgtt gctggagcta caacccgcca tcagtagcgc ggcaccgaca
     9661 atccctgcta agtattttac gtgcatgtta ttccccataa tcaatgagct ggacgccact
     9721 cgcgtcgtcc gtgtctgaat cttatactag aaagatgacc aaaagaaaaa cccccagaca
     9781 tttctgcccg ggggtttgtt tatttctgcg aggtgcaggg caattacatc atgccgccca
     9841 tgccacccat gccgcccata ccgccagcag cgcctaagtc agctgcatca tttttcggca
     9901 ggtcggtaac catgcactcg gtggtgatca tcaggccagc cacagaagcc gcgtactgca
     9961 gagcagaacg ggttactttg gttgggtcca ggatacccat gtcgatcatg ttgccgtatt
    10021 cttcggttgc tgcgttgtaa ccgtagttgc cgtcgccgcc tttaacggtg ttagcaacaa
    10081 cagacggttc ttcgccgcag ttcaggacga tctgacgcag cggagcttcc attgcacgca
    10141 gtgcaacttt gatacccacg ttctggtctt cgttctgacc acgcaggtca gccagtttag
    10201 acgctacgcg gatcagcgca acaccaccac cagcaaccac gccttcttct accgcagcac
    10261 gggtcgcgtg cagggcgtct tcaacgcgtg cttttttctc tttcatttca acttcggtag
    10321 cagcacctac tttgataact gcaacgccgc ctgccagttt cgctacgcgt tcctgcagtt
    10381 tttcacggtc gtagtcagaa gttgcttctt caatctgctg acggatctga gcaacacggc
    10441 cctggattgc agcttcttca cccacgccat cgatgatagt ggtggtgtct ttgttgatca
    10501 caacacgttt agcctgaccc aggtcttcca gggttgcttt ttccagttcc ataccgatct
    10561 cttcagagat cacggtaccg ccagtcaggg ttgcgatatc ctgcagcata gctttacgac
    10621 gatcgccgaa gcccggagct ttaactgcag caactttcac gatgccacgc atggtgttaa
    10681 caaccagagt tgccagcgct tcgccttcta catcttcagc gatgatcagc agcggtttgc
    10741 ctgctttggc aacggcttcc agaaccggca gcatttcgcg gatgttggag attttcttgt
    10801 cagccagcag gatgaacggg ctttccagtt ctactgcgcc agtttccggc ttgttgatga
    10861 agtaaggaga caggtagcca cggtcgaact gcataccttc aaccacgtcc agttcgtcct
    10921 gcagaccggt accgtcttca acggtgataa cgccttcttt accgactttg tccatcgctt
    10981 cagcgatcag tttacctacg gtttcgtcgg agttagcgga gatggtacca acctgagcaa
    11041 tcgctttaga gtcagagcac ggtacggaca gcgctttcag ttcttcaact gcagcggtaa
    11101 cggctttgtc gataccacgt ttcaggtcca tcgggttcat gcccgcagca acagctttca
    11161 gaccctcagt gatgatagcc tgagccagta cagttgcagt ggtggtaccg tcgcctgcag
    11221 cgtcgttcgc tttagaggca acttctttca ccatctgcgc acccatgttt tcgaacttgt
    11281 cttccagttc gatttcacga gcaacggaaa caccatcttt ggtgatggtc ggtgcaccga
    11341 aagatttatc cagaactacg ttacggcctt tcggaccgag ggtaactttc actgcatctg
    11401 ccagtacgtt tacgccgcgc agcattttca cacgagcgtc gttaccgaat tttacgtctt
    11461 tagctgccat tatctttatt ccttaaattc gtatgttcag tgtcgtgcga ggattacgct
    11521 tcaacaattg ccagaatgtc gctttcggac atgatcaaca cttcttcatt gtcgatcttc
    11581 tcagatttca caccgtagcc atcgttgaaa ataacgatgt cgccaacttt cacatccagc
    11641 ggcttcactt cgccattttc aaggatacgg ccattgccga cagccagcac ttcgccgcgg
    11701 gtggatttag ccgctgcaga gccggtcaga acgatgccgc cagcagattt agtttcaact
    11761 tctttacgct tgacgatcac gcgatcatgc aatggacgaa tattcattga taactctcct
    11821 ttgagaaagt ccgtatctgt tatgggtgac gccggagctt acgtggtttc ccggctggtg
    11881 accagagaaa tggggatgag gattcgcccc ttcaaggggg aaaaagaaaa aaaattctga
    11941 tcagcacaaa atcgggtgaa aaccctgatt cacctcacat ttcatcgcaa tttcttcatc
    12001 gccgtcataa gcgaatctga ttgtgctacc atcgaaaatc tacgcagttg cccaaaattt
    12061 gggcgcaatc ggatatcaac ccatgagtgg actcaaacaa gaactggggc tggcccaggg
    12121 cattggcctg ctatcgacgt cattattagg caccggcgtg tttgccgttc ctgcgttagc
    12181 tgcacaggta gcaggcaata acagcctgtg ggcgtggccc gttttgatta tcttagtgtt
    12241 cccgattgcg attgtgtttg cgattctggg tcgccactat cccagcgcag gcggtgtcgc
    12301 gcacttcgtc ggtatggcgt ttggttcgcg gcttgagcga gtcaccggct ggttgttttt
    12361 atcggtcatt cccgtgggtt tgcctgccgc gctacaaatt gccgccgggt tcggccaggc
    12421 aatgtttggc tggcatagct ggcaactgtt gttggcagaa ctcggtacgc tggcgctggt
    12481 gtggtatatc ggtactcgcg gtgccagttc cagtgctaat ctacaaaccg ttattgccgg
    12541 acttatcgtc gcgctgattg tcgctatctg gtgggcgggc gatatcaaac ctgcgaatat
    12601 ccccttcccg gcacctggta atatcgaact taccgggtta tttgctgcgt tatcagtgat
    12661 gttctggtgt tttgtcggtc tggaagcatt tgcccatctt gcctcggaat ttaaaaatcc
    12721 agagcgtgat tttcctcgcg cattgatgat tggtctgctg ctggcaggat tagtctactg
    12781 gggctgtacg gtagtcgtct tacacttcga cgcctatggt gaacaaatgg ccgcggcagc
    12841 atcgcttcca aaaattgtag tgcaactgtt cggtgtagga gcgttatgga ttgcctgcgt
    12901 aattggctat ctgacctgct ttgccagtct caacatttat atacagagct tcgcccgcct
    12961 ggtctggtcg caggcgcaac ataatcctga ccactacctg gcacgcctct cttctcgcca
    13021 tatcccgaat aatgccctca atgcggtgct cggctgctgc gtggtgagca ctttggtgat
    13081 tcatgcttta gagatcaatc tggacgctct tattatttat gccaatggca tctttattat
    13141 gatttatctg ttatgcatgc tggcaggctg taaattattg caaggacgtt atcgactact
    13201 ggcggtggtt ggcgggctgt tatgcgttct gttactggca atggtcggct ggaaaagtct
    13261 ctatgcgctg attatgctgg cggggttatg gctgtttctg ccaaaacgaa aaacgccgga
    13321 aaatggcata accacataat ccggcgtttc gacattaatc ctagcgatcg tctttatgat
    13381 caaggcggtc gcggtcatca tcctttcgct ggtactcacc atcaaaagta ttaccgccac
    13441 cggtcccggc gctaaaaccg ccgcctggca tgcgagaaaa gcgcaaatgt ggcatcaact
    13501 tcactgtcag atgcttttgc accggcggca ataaaagcag aagaccgagg aagtcggtga
    13561 aaaagcccgg taataaaagc agcaaaccgg caataatcag cgaaacactt ttaatcatct
    13621 ccgccgcagg gttttcaccc gccgccattt tttgctgcat cagcacgaaa ttcttaaagc
    13681 cctggttacg taccagtgac ataccgataa ctgacgtgaa tataaccagc acgagggtca
    13741 gcaatacccc caatacatgg gcaacctgaa taaagattga aatctcaata tagacataaa
    13801 ggaaaatggc aataaaaggt aaccagcgca aaggtttctc ctgtaatagc agccggttaa
    13861 acccggctac ctgaatgggt tgcgaatcgc gttcagctta tattgtggtc attagcaaaa
    13921 tttcaagatg tttgcgcaac tatttttggt agtaatccca aagcggtgat ctatttcaca
    13981 aattaataat taaggggtaa aaaccgacac ttaaagtgat ccagattacg gtagaaatcc
    14041 tcaagcagca tatgatctcg ggtattcggt cgatgcaggg gataatcgtc ggtcgaaaaa
    14101 cattcgaaac cacatatatt ctgtgtgttt aaagcaaatc attggcagct tgaaaaagaa
    14161 ggttcacatg tcaaacaaca ttcgtatcga agaagatctg ttgggtacca gggaagttcc
    14221 agctgatgcc tactatggtg ttcacactct gagagcgatt gaaaacttct atatcagcaa
    14281 caacaaaatc agtgatattc ctgaatttgt tcgcggtatg gtaatggtta aaaaagccgc
    14341 agctatggca aacaaagagc tgcaaaccat tcctaaaagt gtagcgaatg ccatcattgc
    14401 cgcatgtgat gaagtcctga acaacggaaa atgcatggat cagttcccgg tagacgtcta
    14461 ccagggcggc gcaggtactt ccgtaaacat gaacaccaac gaagtgctgg ccaatatcgg
    14521 tctggaactg atgggtcacc agaaaggtga atatcagtac ctgaacccga acgaccatgt
    14581 taacaaatgt cagtccacta acgacgccta cccgaccggt ttccgtatcg cagtttactc
    14641 ttccctgatt aagctggtag atgcgattaa ccaactgcgt gaaggctttg aacgtaaagc
    14701 tgtcgaattc caggacatcc tgaaaatggg tcgtacccag ctgcaggacg cagtaccgat
    14761 gaccctcggt caggaattcc gcgctttcag catcctgctg aaagaagaag tgaaaaacat
    14821 ccaacgtacc gctgaactgc tgctggaagt taaccttggc gcaacagcaa tcggtactgg
    14881 tctgaacacg ccgaaagagt actctccgct ggcagtgaaa aaactggctg aagtcactgg
    14941 cttcccatgc gtaccggctg aagacctgat cgaagcgacc tctgactgcg gcgcttatgt
    15001 tatggttcac ggcgcgctga aacgcctggc tgtgaagatg tccaaaatct gtaacgacct
    15061 gcgcttgctc tcttctggtc cacgtgccgg cctgaacgag atcaacctgc cggaactgca
    15121 ggcgggctct tccatcatgc cagctaaagt aaacccggtt gttccggaag tggttaacca
    15181 ggtatgcttc aaagtcatcg gtaacgacac cactgttacc atggcagcag aagcaggtca
    15241 gctgcagttg aacgttatgg agccggtcat tggccaggct atgttcgaat ccgttcacat
    15301 tctgaccaac gcttgctaca acctgctgga aaaatgcatt aacggcatca ctgctaacaa
    15361 agaagtgtgc gaaggttacg tttacaactc tatcggtatc gttacttacc tgaacccgtt
    15421 catcggtcac cacaacggtg acatcgtggg taaaatctgt gccgaaaccg gtaagagtgt
    15481 acgtgaagtc gttctggaac gcggtctgtt gactgaagcg gaacttgacg atattttctc
    15541 cgtacagaat ctgatgcacc cggcttacaa agcaaaacgc tatactgatg aaagcgaaca
    15601 gtaatcgtac agggtagtac aaataaagaa ggcacgtcag atgacgtgcc ttttttcttg
    15661 tgagcagtaa cttaaaaata acaatctaat atcaacttgt taaaaaacaa ggaaggctaa
    15721 tatgctagtt gtagaactca tcatagtttt gctggcgatc ttcttgggcg ccagattggg
    15781 gggaataggt attggttttg caggcggatt gggggtgctg gttcttgccg ctattggcgt
    15841 taaacccggt aacatcccgt tcgatgtcat ttccattatc atggcggtta tcgccgctat
    15901 ttctgccatg caggttgctg gcggtctgga ctatctggtt catcagacag aaaagctgct
    15961 gcgccgtaac ccgaaataca tcacaatcct cgcaccgatc gtgacctatt tcctgacaat
    16021 ctttgctggt actggcaaca tctctctggc gacactgcca gttatcgctg aagttgcgaa
    16081 ggaacaaggc gttaaacctt gccgtccgct gtctactgca gtggtatccg cgcagattgc
    16141 gatcaccgca tcgccaatct cagcggcagt ggtttacatg tcttccgtga tggaaggtca
    16201 tggcatcagc tacctccatc tgctctccgt ggtcatcccg tccaccctgc tggcggttct
    16261 ggtgatgtcc ttcctggtca ctatgctgtt caactccaaa ctctctgacg atccgattta
    16321 tcgcaagcgt ctggaagagg gcctggttga actgcgcggt gaaaagcaga ttgaaatcaa
    16381 atccggtgca aaaacgtccg tctggctgtt cctgctgggc gtagttggcg tggttatcta
    16441 tgcaatcatc aacagcccaa gcatgggtct ggttgaaaaa ccgctgatga acaccaccaa
    16501 cgcaatcctg atcatcatgc tcagcgttgc aactctgacc accgttatct gtaaagtcga
    16561 taccgacaac atcctcaact ccagcacctt caaagcaggt atgagcgcct gtatttgtat
    16621 cctgggtgtt gcgtggctgg gcgatacttt cgtttccaac aacatcgact ggatcaaaga
    16681 taccgctggt gaagtgattc agggtcatcc gtggctgctg gccgtcatct tcttctttgc
    16741 ttctgctctg ctgtactctc aggctgcaac cgcaaaagca ctgatgccga tggctctggc
    16801 actgaacgtt tctccgctga ccgctgttgc ttctttcgct gcggtgtctg gtctgttcat
    16861 tctgccgacc tatccgacgc tggttgctgc ggtacagatg gatgacacgg gtacaacccg
    16921 tatcggtaaa ttcgtcttca accatccgtt cttcatcccg ggtactctgg gtgttgccct
    16981 ggccgtttgc ttcggcttcg tgctgggtag cttcatgctg taatgaccaa tcgcggggcg
    17041 ttcacgcccc gctttctttc ccgccgacta acatccttcc cccgtccgtt gtatagtgac
    17101 ctctctcttg cggttccatc tgttcttgcg aggtgtttat gcttgatgaa aaaagttcga
    17161 ataccgcgtc tgtcgtggtg ctatgtaccg caccggatga agcgacagcc caggatttag
    17221 ccgccaaagt gctggcggaa aaactggcgg cctgcgcgac cttgatcccc ggcgctacct
    17281 ctctctatta ctgggaaggt aagctggagc aagaatacga agtgcagatg attttaaaaa
    17341 ctaccgtatc tcaccagcag gcactgctgg aatgcctgaa gtctcatcat ccatatcaaa
    17401 ccccggaact tctggtttta cctgttacac acggagacac agattacctc tcatggctca
    17461 acgcatcttt acgctgatcc tgctactttg cagcacttcc gtttttgccg gattattcga
    17521 cgcgccggga cgttcacatt ttgtccccgc ggatcaagcc tttgcttttg attttcagca
    17581 aaaccaacat gacctgaatc tgacctggca gatcaaagac ggttactacc tctaccgtaa
    17641 acagatccgc attacgccgg aacacgcgaa aattgccgac gtgcagctgc cgcaaggcgt
    17701 ctggcatgaa gatgagtttt acggcaaaag cgagatttac cgcgatcggc tgacgcttcc
    17761 cgtaaccatc aaccaggcga gtgcgggagc gacgttaact gtcacctacc agggctgtgc
    17821 tgatgccggt ttctgttatc cgccagaaac caaaaccgtt ccgttaagcg aagtggtcgc
    17881 caacaacgca gcgtcacagc ctgtgtctgt tccgcagcaa gagcagcaca ccgcgcaatt
    17941 gcccttttcc gcgctctggg cgttgttgat cggtattggt atcgccttta cgccatgcgt
    18001 gctgccaatg tacccactga tttctggcat cgtgctgggt ggtaaacagc ggctctccac
    18061 tgccagagca ttgttgctga cctttattta tgtgcagggg atggcactga cttacacggc
    18121 gctgggtctg gtagttgccg ccgcagggtt acagttccag gcggcgctac agcacccata
    18181 cgtgctcatt ggcctcgcca tcgtctttac cttgctggcg atgtcaatgt ttggcttgtt
    18241 tactctacaa ctcccctctt cgctgcaaac acatctcaca ttgatgagca atcgccaaca
    18301 gggcggctca cctggcggtg tgtttgttat gggggcgatt gccggactga tctgttcacc
    18361 atgcaccacc gcaccgctta gcgcgattct gctgtatatc gcccaaagcg ggaacatgtg
    18421 gctgggcggc ggcatgcttt atctctatgc gttgggcatg ggcctgccgc tgatgctaat
    18481 taccgtcttt ggtaaccgct tgctgccgaa aagcggcccg tggatggaac aagtcaaaac
    18541 cgcgtttggt tttgtgatcc tcgcactgcc gatcttcctg ctggagcgag tgattggtga
    18601 tatatgggga ttacgcttgt ggtcggcgct gggtgtcgca ttctttggct gggcctttat
    18661 caccagccta caggccaaac gcggctggat gcgcgtggtg caaataatcc tgctggcagc
    18721 ggcattggtt agcgtgcgcc cacttcagga ttgggcattt ggtgcgacgc ataccgcgca
    18781 aactcagacg catctcaact ttacacaaat caaaacggta gatgagttaa atcaggcgct
    18841 cgttgaagcc aaaggcaaac cggtgatgtt agatctttat gccgactggt gcgtcgcctg
    18901 taaaaagttt gagaaataca ccttcagcga cccgcaggtg caaaaagcgt tagcagacac
    18961 ggtcttactt caggccaacg tcactgctaa cgacgcacaa gatgtggcgc tgttaaagca
    19021 tcttaatgtc cttggactac cgacaattct cttttttgac ggacaaggcc aggagcatcc
    19081 acaagcacgc gtcacgggct ttatggatgc tgaaaccttc agcgcacatt tgcgcgatcg
    19141 ccaaccgtga acgacacttg cagtgggaaa gacggaggaa aatagcgtgc aacgtgaaga
    19201 tgtactggga gaagccctga aattattaga attacaaggg attgccaaca ccacgctgga
    19261 gatggttgct gaacgtgtgg attatccact ggacgagcta cgccgcttct ggccagacaa
    19321 agaggcaatc ctctacgatg cgctgcgtta tcttagccaa cagatagatg tctggcgtcg
    19381 tcagctgatg cttgacgaaa cgcaaaccgc cgaacaaaag ctgctggcac gttatcaggc
    19441 gttatcggag tgcgttaaaa acaaccgcta tccgggctgt ctatttatcg ctgcctgtac
    19501 gttttatccc gaccctggcc accctattca tcaactggcc gatcagcaaa aaagcgcggc
    19561 ctacgatttc acccacgaac tgttaaccac gctggaagtt gacgatccgg cgatggtagc
    19621 aaagcagatg gaactggtgc tggaaggctg tttaagccga atgctggtga atcgtagcca
    19681 ggcggatgtc gacaccgcac atcggctggc ggaagatatc ctgcgcttcg cccgctgccg
    19741 ccagggtggt gcactgacct gacagaaaca cagaaaagaa gcgatttgcc gcaatcttaa
    19801 gcagttgaat cgcttttact gaaattaggt tgacgagatg tgcagattac ggtttaatgc
    19861 gccccgttgc ccggatagct cagtcggtag agcaggggat tgaaaatccc cgtgtccttg
    19921 gttcgattcc gagtccgggc accaaattca tataaacgga cctccacgga ggtccgtttt
    19981 tcgtttcagg acgccacgat ttaagcgttc tgcctccaaa tcaattctac cgaactcaac
    20041 cagattctcc ccacatcacc agcattttgc gggtatatcc caattcggga aaatttgttc
    20101 ctgagcgata gcactgactg acgtgaaatg tcgtgcggcc ccgtgatgtt gttgaacgtc
    20161 aaatgacgtc atcaggagcg taacgcatcc ataaagcaca acaccggtct gaacggcgac
    20221 tgatgagatt ttctgaatga gaacaaagag aaatgtatca gtccgcttgc tcatgcaaag
    20281 actaacaatc cattaaaata gtaagcgctc cggacaattt tccatggatt attttctaca
    20341 catttttctt tggcaaagat gatgaatttt gatggtaagg aatattactt ctggttctca
    20401 gtaaaatcct ttcgtaatac tatgtaatca agaagtttat ggctggtaaa aataacgtct
    20461 tgcattcacc aataacatgt aaataaaccc atctattgat ggtaaaaata ggtcatggca
    20521 attatcattg caccattccc ttttcgaatg agtttctatt atgcaacaac ctgtagttcg
    20581 cgttggcgaa tggcttgtta ctccgtccat aaaccaaatt agccgcaatg ggcgtcaact
    20641 tacccttgag ccgagattaa ttgatcttct ggttttcttt gctcaacaca gtggcgaagt
    20701 acttagcagg gatgaactta tcgataatgt ctggaagaga agtattgtca ccaatcacgt
    20761 tgtgacgcag agtatctcag aactacgtaa gtcattaaaa gataatgatg aagatagtcc
    20821 tgtctatatc gctactgtac caaagcgcgg ctataaatga atggtgccgg ttatctggta
    20881 cagcgaagaa gagggagagg aaataatgct atcttcgcct ccccctatac cagaggcggt
    20941 tcctgacaca aattctccct cccacagtct taccattcaa aacaccgcaa cgccacctga
    21001 acaatcccca gttaaaagca aacgattcac taccttttgg gtatggtttt tttcctgttg
    21061 tcgttaggta tctgtgtagc actggtagcg ttttcaactc ttgatacacg tcttcctatg
    21121 agcaaatcgc gtattttgct caatccacgc gatattgaca ttaatatggt aaataaaagt
    21181 tgtaacagct ggagttcccc gtatcagctc tcttacgcga taggcgtggg tgatttggtg
    21241 gcgacatcac ttaacacctt ctccaccttt atggtgcatg acaaaatcaa ctacaacatt
    21301 gatgaaccga gcagttccgg taaaacatta tctattgcct ttgttaatca gcgccaatac
    21361 cgtgctcaac aatgctttat gtcggtaaaa ttagtagaca atgcagatgg ttcaaccatg
    21421 ctggataaac gttatgtcat cactaacggt aatcagctgg cgattcaaaa tgatttactg
    21481 gagagtttat caaaagcgtt aaaccaaccg tggccacaac gaatgcagga gacgctccag
    21541 caaattttgc ctcatcgtgg tgcgttatta actaattttt atcaggctca tgattattta
    21601 ctgcatggcg atgacaaatc attgaatcgt gccagtgaat tattaggtga gattgttcaa
    21661 tcatccccag aatttaccta cgcgagagca gaaaaagcat tagttgatat cgtgcgccat
    21721 tctcaacacc ctttagatga aaaacaatta gcagcactga acacagaaat agacaacatt
    21781 gttacactgc cggaattgaa caacctgtcc attatatatc aaataaaagc ggtcagtgct
    21841 ctggtaaaag gtaaaataga tgagtcttac caggcgataa atactggcat tgatcttgaa
    21901 atgtcctggc taaattatgt gttgcttggc aaggtttatg aaatgaaggg gatgaaccgg
    21961 gaagcagctg atgcatatct cactgccttt aatttacgcc caggggcaaa caccctttac
    22021 tggattgaaa atggtatttt ccagacttct gttccttatg ttgtacctta tctcgacaaa
    22081 tttctcgctt cagaataagt aactccgggt tgatttatgc tctggaatat ttgttgttga
    22141 gtttttg
//
LOCUS       DAZRUF010000047        21940 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZRUF010000047 DAZRUF010000000
VERSION     DAZRUF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.047"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHXOL_001442"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHXOL_001442"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR6808459.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHXOL_001443"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHXOL_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR6808460.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHXOL_001444"
     CDS             complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHXOL_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR6808461.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="ACHXOL_001445"
     CDS             2284..2730
                     /locus_tag="ACHXOL_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR6808462.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3507)
                     /gene="nimR"
                     /locus_tag="ACHXOL_001446"
                     /pseudo
     CDS             complement(2687..3507)
                     /gene="nimR"
                     /locus_tag="ACHXOL_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3604..4785
                     /gene="nimT"
                     /locus_tag="ACHXOL_001447"
     CDS             3604..4785
                     /gene="nimT"
                     /locus_tag="ACHXOL_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR6808463.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4840..5187
                     /locus_tag="ACHXOL_001448"
     CDS             4840..5187
                     /locus_tag="ACHXOL_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR6808464.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5209..5463)
                     /gene="yoaF"
                     /locus_tag="ACHXOL_001449"
     CDS             complement(5209..5463)
                     /gene="yoaF"
                     /locus_tag="ACHXOL_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR6808465.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5646..6671
                     /gene="dgcP"
                     /locus_tag="ACHXOL_001450"
     CDS             5646..6671
                     /gene="dgcP"
                     /locus_tag="ACHXOL_001450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR6808466.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6704..6802
                     /locus_tag="ACHXOL_001451"
     CDS             6704..6802
                     /locus_tag="ACHXOL_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR6808467.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6805..6879
                     /gene="yoaJ"
                     /locus_tag="ACHXOL_001452"
     CDS             6805..6879
                     /gene="yoaJ"
                     /locus_tag="ACHXOL_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR6808468.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6938..7186)
                     /locus_tag="ACHXOL_001453"
     CDS             complement(6938..7186)
                     /locus_tag="ACHXOL_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR6808469.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7334..7516)
                     /locus_tag="ACHXOL_001454"
     CDS             complement(7334..7516)
                     /locus_tag="ACHXOL_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR6808470.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7520..7879)
                     /gene="yeaR"
                     /locus_tag="ACHXOL_001455"
     CDS             complement(7520..7879)
                     /gene="yeaR"
                     /locus_tag="ACHXOL_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR6808471.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8052..8690)
                     /gene="leuE"
                     /locus_tag="ACHXOL_001456"
     CDS             complement(8052..8690)
                     /gene="leuE"
                     /locus_tag="ACHXOL_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR6808472.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8817..9740)
                     /gene="dmlR"
                     /locus_tag="ACHXOL_001457"
     CDS             complement(8817..9740)
                     /gene="dmlR"
                     /locus_tag="ACHXOL_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR6808473.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9843..10928
                     /gene="dmlA"
                     /locus_tag="ACHXOL_001458"
     CDS             9843..10928
                     /gene="dmlA"
                     /locus_tag="ACHXOL_001458"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR6808474.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11179..12789
                     /gene="yeaV"
                     /locus_tag="ACHXOL_001459"
     CDS             11179..12789
                     /gene="yeaV"
                     /locus_tag="ACHXOL_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR6808475.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12821..13945
                     /gene="yeaW"
                     /locus_tag="ACHXOL_001460"
     CDS             12821..13945
                     /gene="yeaW"
                     /locus_tag="ACHXOL_001460"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR6808476.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14001..14966
                     /gene="yeaX"
                     /locus_tag="ACHXOL_001461"
     CDS             14001..14966
                     /gene="yeaX"
                     /locus_tag="ACHXOL_001461"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR6808477.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGISITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15020..16135)
                     /gene="rnd"
                     /locus_tag="ACHXOL_001462"
     CDS             complement(15020..16135)
                     /gene="rnd"
                     /locus_tag="ACHXOL_001462"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR6808478.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16217..17902)
                     /gene="fadD"
                     /locus_tag="ACHXOL_001463"
     CDS             complement(16217..17902)
                     /gene="fadD"
                     /locus_tag="ACHXOL_001463"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR6808479.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18107..18688)
                     /gene="yeaY"
                     /locus_tag="ACHXOL_001464"
     CDS             complement(18107..18688)
                     /gene="yeaY"
                     /locus_tag="ACHXOL_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR6808480.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18728..19423)
                     /gene="tsaB"
                     /locus_tag="ACHXOL_001465"
     CDS             complement(18728..19423)
                     /gene="tsaB"
                     /locus_tag="ACHXOL_001465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR6808481.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19481..21391)
                     /locus_tag="ACHXOL_001466"
     CDS             complement(19481..21391)
                     /locus_tag="ACHXOL_001466"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR6808482.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            21523..21867
                     /locus_tag="ACHXOL_001467"
     CDS             21523..21867
                     /locus_tag="ACHXOL_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR6808483.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5361 a         5640 c         5360 g         5579 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt actatcagtc gcgcaagatt
     2941 gcgttcactc atcgcaaaaa agccagccca ttgccctaat gacccccact cgacgggccc
     3001 tttcgccatc atctccacca tcgtgcggat tttaggatga gaagaaacgg gcaggtgcag
     3061 ctgttgttgt ggctgctgag ggagttcatc aaacaggacc tgaattaatc tgcgggtcat
     3121 cggctcagca cgctgagtcg tcgtcctgtt tgccagggtt aaaatcaatt cccggcataa
     3181 gggcgatatt ttcaatgtgc aacatgttgt cggcattgtc actgcgctgg gttcgataaa
     3241 cagaaaacag agttcagcgt tagctgtcac ttgattgcta tgttccaccc cgcccggtat
     3301 ccaaaccgca tattgcggcg gcaccatcca caaagcattt tccaccgtac aggtaattgc
     3361 accatgtaat gctagtatca gctgtccttt gcggtgctga tgtacaggac tggttaactc
     3421 atccgttccg gcgtgaatac aaaatgcgac cgcggcttca tggtgtcggt cgggttcata
     3481 gccattgaga ttcaacctgt gcatcatttt gtccgaattt agcgataatt tgtcatttta
     3541 gcttgattca acataacaat aaaaacggta aggtacagcc tcgtttgtaa caatgagaag
     3601 catatgacct gttcaacttc attaagcggc aaaaacagga ttgtccttat cgctggcatt
     3661 ctgatgattg ccacaacatt acgcgtcacc tttatcggcg cagcaccgtt actggatacg
     3721 attcgttccg cttactcgct gacgacagcg caaaccggct tattgaccac cctgccatta
     3781 ttggcctttg cgctaatctc acctttggcc accccggtag cgcgacgttt tggtatggaa
     3841 cgtagcctgt ttgccgcgtt acttttgatc tgtgctggta tcgcaattcg ctctctccct
     3901 tcgccttact tattatttgg cggtacagcg gtcattggcg gtgggattgc attaggcaat
     3961 gtcttactgc caggattaat taaacgcgat ttccctcatt ccgtcgccag acttaccggc
     4021 gcatattccc tgacaatggg agctgcagcg gcactgggat cggctatgat agtgccgctg
     4081 gctttgaacg gttttggctg gcaaggcgcg ttgctcatgc tgatgtgttt tcctctgctg
     4141 gctctttttt tatggctgcc acagtggcga agccaacaac atgcaaattt gagtacctcg
     4201 cgcgccttac atactcgggg tatctggcgt tcgccgcttg cctggcaggt aacattgttt
     4261 cttgggatca actcactgat ctattacgtg attattggct ggcttccggc gatcctcatc
     4321 agtcacggct atagcgaagc acaggcgggt tcactgcatg gtttgctgca actagccaca
     4381 gcagctcccg gtttgctgat ccctcttttc ttacatcatg tgaaagatca gcgtggtatt
     4441 gcagcgttcg ttgccttgat gtgcgcagtg ggcgctgttg ggctctgctt tatgccagcg
     4501 cacgcgatca cctggactct gcttttcggt tttggttccg gcgcaacaat gatactgggg
     4561 ttgacgttca ttggtcttcg ggcaagttct gcgcatcagg cggcggcact ctcggggatg
     4621 gcacaatccg tcgggtattt gctggcagcc tgtgggccgc cgctaatggg taaaatacac
     4681 gatgctaacg gtgactggtc cgtaccactg atgggtgttg caatactttc actactgatg
     4741 gcgattttcg gactttgcgc cgggagagac aaagaaattc gctaatatcc ggtgctatag
     4801 tgacgtgaca aatcatgcgt gaaagggaga acaaacacga tgaatattca gtgcaaacgc
     4861 gtttatgatc cggctgaaca gagcgatggt tatcgcgtac tggtcgaccg cctctggccg
     4921 cgcggtatca aaaaaaccga tttagccctt gatgagtggg ataaagaaat cacgccgtca
     4981 acggaactgc gcaaagcctt tcacggcgaa gtcgtcgatt ttgcaacctt tcgcgagcaa
     5041 tatcttgcag aactggcgca acacgagcaa gaaggaaagc ggctggcgga catcgccaaa
     5101 aaacagccgc tgaccctgct ctacgcagca aaaaacacca cgcagaacca tgcgctggtg
     5161 ctggccgact ggctacgtag cttgtgattt tagtacagca tccggcggtt atttttcacc
     5221 agccggatgg tcccgtcgcc acaatgccca ttcatcaatc gtttcaccgc ccggtaattt
     5281 gcaattgttg ctgacccctt gcgctgtcga cactggaatg agcgtcccgc ccttctgctg
     5341 gcaatagacc gacgccggat ttgccatacc aatctgcggc ggtttaggta cttctggctg
     5401 agaagaggtt gaacaaccag ccaggaccag caagcaaggc aaagcaaaac tgataatttt
     5461 catttattga tctcactcat ttatctacgc ttagagtatc gcggtatcgt tttgttttgc
     5521 agcactattt ttattacatt cactcaaaac atattacgtc ttgtttcatc tttgttggtg
     5581 atgttttatc atgcctgcaa agattaaata atcagcattt acccgccgta tcctggagtt
     5641 gtttcgtgtc agatcagatt atcgcccgcg tctcgcaatc ccttgccaaa gaacagtcac
     5701 tggaaagtct ggtccgacag cttctggaga tgctggaaat ggtcactgat atggaatcaa
     5761 cctacctgac caaagtggat gtcgaagcgc gcctgcagca tattatgttt gcccgtaaca
     5821 gccagaaaat gcacatcccg gagaatttta ccgtctcgtg ggattactcg ttatgcaaac
     5881 gcgccattga tgaaaactgc tttttcagcg atgaagtccc tgaccgttgg ggcgactgta
     5941 ttgcggcacg caatcttggc atctccacat ttctgagcac accaattcac ttaccggatg
     6001 gatcattcta tggcacgctt tgcgccgcca gcagtgagaa gcgccagtgg agtgaacgcg
     6061 cggaacaggt tttacagtta ttcgccggac tgattgcaca atatattcaa aaagaggcac
     6121 tggttgaaca gttgcgcgaa gccaatgctg cgctgattgc gcaatcgtat accgactcgt
     6181 taaccgggtt gccgaatccg cgggcgattt ttgaaaatct gacgacgctg ttttctctcg
     6241 cccggcatct taaccataag ataatgatcg cgtttatcga tctggataac ttcaaattaa
     6301 tcaatgatcg ttttggtcat aatagtggcg atctgtttct cattcaggtt ggcgagcgcc
     6361 ttaatacgct ccagcaaaat ggcgaagtta ttggtcgtct cggcggtgat gagtttttgg
     6421 ttgtttcact aaacaacgag aatgcggata tttcgtcgct gcgagaacgc attcagcagc
     6481 aaatacgtgg agaatatcac ttaggtgatg ttgatttgta ttatcccggt gccagtcttg
     6541 gcatagtaga agtcgatcct gaaacaaccg atgcagacag tgccctgcat gctgccgata
     6601 ttgcgatgta tcaggagaaa aaacacaaac agaaaacacc ttttgtcgcg cattcagcgc
     6661 cacattgctg aggcgtattc acatcctttt gattggtgat aacatgcgaa tcggtattat
     6721 ttttccggtt gtaatcttta ttacagcggt cgtattttta gcatggtttt ttattggcgg
     6781 ctatgctgcc ccgggggcat aaagatgaaa aaaacaacga ttattatgat gggtgtggcg
     6841 attattgtcg tactcggcac tgagctggga tggtggtaac gtcacctcta aaaaatagca
     6901 aaggctgcct gtgtgcagcc tttgtgcaat ttaagcgtta acttttaatc ttcctgtaga
     6961 taaatagcac gacaatcgca ccaataacgg caaccacgaa gctgccaaaa ttgaagccat
     7021 cgactttacc aaagccaaac agcgtgctga tccatccgcc gactacggca ccgactatcc
     7081 ccagcaggat agtcataaag aatccaccac catctttacc tggcatgatc cacttcgcca
     7141 gaataccggc aataagccca aaaataatcc atgacagaat gcccattgtt tcctcactta
     7201 tctgttttgc attagcgggt tagtcgctga taaaaagcat agcacaacat cgggagggca
     7261 agatttgtga cgagcatcac ggaggatttt tttgcgatgg cgcagaaatt gcgccatcaa
     7321 cgatcagtga taattaccaa ccacaaacat catgttcgtt ttccgtgtca taagaacgca
     7381 cggtattcac cagatctttt atcacttcag ccgccacttc tggcaccagc aaagtcatcg
     7441 gcgtctctgt ttcataatcg acagaaacgc cattactgtt attggtgacg gtcacggtat
     7501 acgttgcttt gcccatgatt catttcccgt tatgaatgac tttccgttgt tgcgcacctt
     7561 ccatcaggac ttcaggagcc acgaagaagt caatgttgaa ataagtatcg tcagtcatgg
     7621 cttcaatgtt gtgccacttt tctggaggga acacctcaaa ctgccccgct tcgataagga
     7681 tcacctgatc aggctctgca ctgtgttcat cagcgtagcc gagatatttg accgccccat
     7741 gcataacgga aaggcgtggg taaacccccg ggcgcgttcc tttatcaaga tgacgttcga
     7801 atattccggc aggcgcagtt tgtttattcc agaaaggcgt tgagcgcgta tgaatataat
     7861 tctgtgggat ttgcagcatc cttttccctc cttcggtgaa tgcgctgaaa acggtttatt
     7921 ccagccgttt cagggtacgc ctgataattt gcattttaaa taccatttat tggttacttt
     7981 ttagcaccat atcagagaaa aatcagggag gattacaagt gggaagcccg cgacaacgcg
     8041 ggctgaatgt atcaggattg cagcgtcgcc agtcgggcgg cgaaacccac gaacatcaaa
     8101 ccaatcagtg agttgccaac tttagccagt ttctttttgg tacgtatgta ctgcgtgaca
     8161 aaagcaccag aaataatcag gaagctcaaa tagcagaaac tcaccagttc cagcgtcgtc
     8221 gccagaataa agaatgaaat tcccgtatgt ggggcattaa catcgataaa ctgtacgaaa
     8281 aacgacacat agaacaaaat ggctttcgga ttagtcaggc tcaaaattaa cgcgcgttta
     8341 aaaatggccc cgtattgggg ctcatcggat ttggcctcgt tatttttacc cttcagggtc
     8401 gcgtagagaa ttttactccc cagatagagc aaataaaacg caccaagata acgtacaatg
     8461 ttgaataata tcggggtggt cttaattaat gtcgccactc cagcccatgc cagaaacatc
     8521 aatactgcat cgccaataaa tacaccgcag gccgcaagat aaccgccttt cataccgcta
     8581 ctgacgctat ttttgagtac aaacagggta tttggccctg gcaccaacac aataaaaatg
     8641 gccccaacca gataggtcca gtaattcaga accccgtatt cagcgaacac attaacctct
     8701 ttaattatct ttcgatcatg cgcgattaaa ggtgaatatg ctaaccaatc tgtagcggct
     8761 tagaaaggag aaaatcaggt tttaacccga tatcaacccg ataattgaat aattaatcag
     8821 gcatgctcca gtgaaaaatt cgggtagtgc tcggcaaaat actggcgtaa aaattctatc
     8881 gttatccgca ctttcgctga cgtcgccagc cttgaaacat aaacggccca gacgttcgct
     8941 ggctggtaat attccggtag cacttgcact aaatgaccac tggcaatgtt ttcgctaaca
     9001 tcccaccagg agcgcagagc aatcccctgc ccatcgagac accactgatg gacaatttct
     9061 ccatgattag acgacagtgg gccggtgact ttaatcgcat ggtggccttc tttattgcgt
     9121 aattgccaga caccaaaagg atgatcgcgc tctttaatga ccaggcacgg caatgcggaa
     9181 agatcggtta aatgtttcgg cgcgccatgt tgcgcaataa attccggcga cgcgcagaga
     9241 atgcggtaat tagtcgccag tttgcgggcg attaaattag gggcgatgtc atcgccaatg
     9301 cgaatatcga gatcaacacc ttcattgacc aaatcgacca accggtcttc cacatcaaaa
     9361 cgtaattcca gttgcggata cgccttagcc agcgctgata acgccggagc caccacctgt
     9421 cgtccaaaac caaagctgct gataatacgc agcatcccct gcggcacctg acgcacgtca
     9481 gaaagttcgt ccatcatctg accgacatcc tgcaaaatcc gctgcgccca ttcataaatc
     9541 cgctctcctt cttcggtaat ggtgacgcgg cgggtggtgc ggtgtaacaa caccacgttt
     9601 agcgtttgct ccagcaaggc gatgcgcttg ctgacgaacg ccggtgaaac gcccagttct
     9661 tccgccacgg cggcaaaacc agcccggcga gccaccagca taaagacgcg caaatcgttc
     9721 agcagcggta aattattcat gattcgtgtt ttatgtttca ccagttacgg ggattaattc
     9781 cttttcagtc aattatagga tggtgatgtt gtcaattttg atggtcagga agtgagaacc
     9841 caatgatgaa aacgatgcgt attgctgcga tcccgggaga tgggattggc aaagaagtcc
     9901 ttcctgaagg gattcgcgta ttacaggctg ccgctgagcg ctggggcttc gacttgagtt
     9961 ttgagcaaat ggagtgggcg agctgcgagt attacagcca tcacggtaaa atgatgccgg
    10021 acgactggca tgagcaactt agccgtttcg acgccatcta ttttggtgcc gtcggctggc
    10081 cggataccgt tccggaccat atttcgttgt ggggttcgct gctgaaattt cgtcgtgaat
    10141 tcgaccagta cgtcaacctg cgcccggttc gtctctttcc tggcgttccc tgcccgctgg
    10201 cgggaaaaca gcctggcgac atcgattttt acgtggtcag ggaaaacacc gaaggcgaat
    10261 attcctcgct cggcggtaga gtgaatgaag gtacagagca tgaagtcgtc attcaggaat
    10321 cggtatttac gcgtcgtggt gtcgatcgca ttttgcgtta tgccttcgaa cttgcgcaaa
    10381 gccgcccacg taagacgcta acttctgcca ctaaatcaaa cggtttagcc atcagcatgc
    10441 cgtactggga tgagcgagtg gaagcaatgg ccgagaatta cccggagatc cgctgggaca
    10501 agcagcatat tgatattctc tgcgcgcgtt ttgtgatgca gccggaacgc ttcgatgtgg
    10561 tggtggcgtc caatttgttt ggcgatatcc tttccgatct tggcccggcc tgcaccggca
    10621 ccattggcat tgccccatcc gccaacctga atccggaacg cactttcccg tcgctcttcg
    10681 agcctgtcca cggttccgcg ccggatatct acgggaaaaa tattgctaac cctatcgcca
    10741 caatttgggc cggtgcgatg atgctcgatt ttctcggcaa tggcgatgag cgtttccagc
    10801 aagcgcataa cggtattctg gcagcgattg aagaagtgat tgctcacggg ccgaaaacac
    10861 cggatatgaa aggcagtgcc accacgccac aggttgccga cgcgatttgc aaaattattt
    10921 tgcgttaagg tcaaaccagt ttatttgaac cgcgtcactg acgcggtttt tttattcgtt
    10981 ctttgcagtt aataacctgc gtcatttcac cttttattgt ttccgtttcg tgttttatgg
    11041 ctttccgtat tcttaattgt ttaatttatg taacatgcaa ataatcaatt tgttaaacac
    11101 aacatacaac tcattaccct gcaaataatt acaactggca aaagccagag acgggctttt
    11161 atgcccagga gatgactcat gatgagcaat gttaagaaaa aagatgtgcc gctgataagc
    11221 atcagcctgg tggccattct tttcatcgca gctgcattaa gccttttccc acaacaatcg
    11281 gccgacgcgg ccaacacaat atacactttt gttacgcgta cgttaggttc cgccgtacag
    11341 gtattggttt tgctggcaat gggactggtg atttatttag ccaccagtaa atacggcaat
    11401 attcgtcttg gcgaaggaaa accggaatac agcacgctct cctggctgtt tatgtttatt
    11461 tgtgccggtt taggttcttc tacgctttat tggggggttg ctgaatgggc ctattattat
    11521 caaacgcctg gattaaatat cgcaccacgt tcacaacagg cactcgaatt tagcgtccct
    11581 tactccttct tccactgggg catcagcacc tgggcaactt atacgctggc ctcattaatc
    11641 atggcttatc actttcatgt gcgtaaaaac aaaggtctga gtctttccgg cattattgcc
    11701 gcgattaccg gcgttcgccc gcaaggccca tggggaaaac tggttgattt gatgtttctg
    11761 atcgccactg tcggcgcact gactatttcc cttgttgtca ccgcagcaac ctttactcgt
    11821 ggactttccg cgctgaccgg tttacccgat aacttcaccg tgcaggcatt tgtgatcctg
    11881 ctttccggcg gcattttttg cctaagctcg tggattggta tcaacaacgg tttgcaacgt
    11941 ctgagcaaaa tggttggctg gggcgcgttc ctgctgccat tactggtgct gattgtcggt
    12001 ccaaccgaat ttattaccaa cagcatcatc aatgccatcg gcctgaccac gcaaaacttc
    12061 ctgcaaatga gcttattcac cgatccgctt ggcgatggtt catttacccg caactggacc
    12121 gttttctact ggctgtggtg gatctcatac acccctggcg tagcaatgtt tgtcacccgc
    12181 gtttcccgcg gtcgtaagat taaagaagtt atctggggac tgatcctcgg cagcaccgtc
    12241 ggttgctggt tcttcttcgg cgtaatggaa agctatgcca ttcatcagtt tatcaatggc
    12301 gtaatcaacg tcccacaggt gctggaaaca ctgggcggcg aaacagctgt acagcaagtt
    12361 ctgatgtcgt tgccagccgg taaattgttc ctcgccgcat acctgggcgt gatgattatt
    12421 ttccttgcct cgcatatgga tgcagtggcc tacaccatgg cggcgaccag tacgcgtaat
    12481 ctccaggaag gtgacgatcc tgaccgtggg ctgcgtcttt tctggtgcgt ggtgatcact
    12541 ctgatcccgc tttccatctt gtttaccggt gcttcgctgg aaacgatgaa aaccaccgtc
    12601 gtgctcacag cccttccctt cctcgtcatt ttactggtga aagtcggcgg atttattcgc
    12661 tggctgaaac aggattatgc cgacattccg gctcatcaag ttgaacatta tctcccgcag
    12721 acaccggttg aagccctgga aaaaacacca gtgctccctg cgggaaccgt attcaaaggc
    12781 gacaactgag cgccatcatc ctaacgataa aggtatccct atgagcaatc tgagccctga
    12841 ctttgtacta cccgaaaatt tttgcgctaa cccgcaagag gcgtggacca ttcctgcccg
    12901 tttttatacc gatcagaacg cgtttgaaca cgaaaaagag aacgtcttcg ccaaaagctg
    12961 gatttgcgtc gctcacagca gcgaactggc gaatgccaat gattatgtga cgcgtgagat
    13021 cattggcgaa agcatcgtgc tggtacgcgg tcgtgataag gttttgcgcg cgttctataa
    13081 cgtgtgtccg caccgtggtc atcagttgtt gagcggtgaa ggaaaagcaa aaaatgtgat
    13141 tacctgccca tatcacgcat gggcattcaa actcgatggc aatctggccc atgcacgtaa
    13201 ctgcgaaaac gtcgccaatt tcgatagcga caaagcgcaa ctggttccgg tgcgtctgga
    13261 agaatatgcc ggattcgtct tcatcaacat ggaccccaac gccaccagcg tagaagatca
    13321 gttacccggt ctgggcgcga aagtgctgga agcctgcccg gaagtccacg atctgaaact
    13381 ggcggcccgc tttaccaccc gcacgcctgc caactggaag aacattgtcg ataactatct
    13441 cgagtgctat cactgtggtc cggcgcatcc aggtttctcc gactccgtac aggttgatcg
    13501 ctactggcac accatgcacg gtaactggac gctgcaatac ggtttcgcca aaccgtccga
    13561 acagtcgttt aaatttgaag agggtacgga tgcggcattc cacggtttct ggctgtggcc
    13621 gtgcacgatg ctgaacgtca ccccgatcaa agggatgatg acggtcattt atgaattccc
    13681 ggtggattct gaaactaccc tgcaaaacta cgatatttac ttcaccaatg aagagttaac
    13741 cgacgagcaa aaatcgctga ttgagtggta tcgcgatgtg ttccgtccgg aagatttacg
    13801 tctggttgaa agcgtacaga aagggctgaa atcgcgtggc tatcgtggtc aggggcgcat
    13861 catggccgac agtagcggta gtggcatttc cgaacatggt atcgcccatt tccataatct
    13921 gctggcgcag gtgtttaagg actaatgaca tcggcggcgg tattttccgc cgctgggctg
    13981 atttttgatg gagtacagca atgtcagact atcaaatgtt tgaagtacag gtgagtcagg
    14041 tggaaccgct taccgaacag gtgaaacgct tcacgctggt ggcaaccgat ggcaaaccgc
    14101 tgcctgcgtt caccggagga agtcacatca ttgtgcagat gagcgatggt gataaccagt
    14161 acagcaatgc gtattcacta ctgagttcgc cgcatgacac ctcttgttat cagattgccg
    14221 ttcggctgga ggaaaactcg cgcggcggtt cccgcttttt gcatcagcag gtaaaagtgg
    14281 gcgatcggtt aacgatttca acgcctaata acctgtttgc gctaattccc tcagccagaa
    14341 agcatctgtt tatcgcgggc ggtattagta tcaccccttt cctgtcgcac atggcagagt
    14401 tgcaacacag cgacgtcgac tggcagctac attactgctc gcgaaatcca gaaagttgcg
    14461 catttcgtga tgagctagtc cagcatccgc aggctgagaa agtccatttg catcattcat
    14521 caaccggaac acgactggaa ttagcgcgat tattggcgga tatcgaacct ggcacacacg
    14581 tttatacctg tggccccgag gcgctaaatg aagcggtaag aagtgaagct gcgcgtctgg
    14641 acatcgccgc cgatacgctg cactttgagc aatttgctat cgaagacaaa accggcgatg
    14701 cctttaccct ggtgcttgcc cgttccggaa aagagtttgt ggtgccggaa gagatgacta
    14761 ttttgcaggt tattgaaaat aataaagccg cgaaagtgga atgtttatgt cgtgaagggg
    14821 tatgcggaac ctgcgaaaca gcaatactgg aaggtgaagc tgaccatcgg gatcagtatt
    14881 ttagcgatga agagcgtgcc agccagcaaa gtatgttgat ctgttgttcg cgtgcgaagg
    14941 gtaaacgcct ggtgttggat ttgtagtttg ccaaagccgg atgtggcgct gagcgcgccc
    15001 agtccggctt cggaagattt tactgcggat attcctgcaa taaattgtgt aatgcttccg
    15061 ccatcagctc accacgccag ccggaaatca gctccggcaa attgttctgc ggtttcagtt
    15121 tccagtgcca gttcagcagt tggttgattt gccgacgcga tgccagcaat tcggcgctga
    15181 tcttatgcgt ttcgctcacg tcagtaatca gcgacttaat cgctttaaac gctttacgat
    15241 aacccggcat gtccatcagg ttaagcatcg gctgcggtaa ggcctcttcc ggcaatgtct
    15301 gcgctttttc caccagcgcc agcagcgttt taccgtgaaa gcggatttcg ctaccggata
    15361 aacccaggct gtccagttcg cctaaacttc ccggcatata acgcgcgacc gaccacaaat
    15421 gctcttcacg cacgacaaag ttcaccgcca gatcacgctc acgagccttg cgcagtcgcc
    15481 agtcggctaa cagttgcaga caggccagtt ggcgtgtgcg taattgccag gcattggtga
    15541 tatcacgcca ggcatcttcc ggcgcaacga cttcctgacg acgcatttgc atcaggcggc
    15601 attcatccag cgccgcaggt agccagccgg aggcctccgt ttctaccata agcttggcgg
    15661 tgatcggtaa cagataccag acatccgccg ctgcgtattc acactgacgt tcggtcagcg
    15721 gtctggccag ccagtcggtg cgcgattcac tcttgtccag cgtaacgccg gaatactctt
    15781 ccaccatgga agcgaaaccc catgacatcg ggcgtccgca gaaggcagca aggatttgcg
    15841 tgtcaatcag gggttgtggt aattcgccaa agacattgag gaacacttcc agatcttcac
    15901 tgcctgcatg gagaaatttt gtgatggacg gatcgcgcag gatcgctttc agcggtgacc
    15961 agtcggtgat cccgagagga tcaattagcg ccagatgctc accatcgaaa agttgaatca
    16021 accccagctg cgggtaataa gtgcgcgtac gaacaaattc agtatcctgg gctatcgccg
    16081 gaaagacacg gacggcttca cacaaagaag ccagcgcatc gtccgtggta atcatttggt
    16141 aattcaaatt gtattctctt tagtgggcgt caaaaaaaac gccggattaa ccggcgtctg
    16201 acgactgact taacgctcag gctttattgt ccactttgcc gcgcgcttcg tcacgtaatt
    16261 ctcgtcgcaa aatttttccg acgttagatt tcggtaactc atcacgaaac tccaccagct
    16321 tcggtacttt gtagcccgtg agctgacggc agcaaaaggt caccagtgac tcttcggtaa
    16381 gcgatggatc ttttttcact acgaagattt tcaccgcttc accactggag ccggaaggta
    16441 cgccaacagc cgcgacttcc tgtacgccag gatgctgcat gacgacatct tcaatctcgt
    16501 tgggatagac gttaaaaccg gaaaccagaa tcatgtcttt tttacgatcg acaatgcgca
    16561 ggaacccttc ttcatccatc accgcgatgt cgccggtgtg taaccagcca tttttgatga
    16621 tctcatcggt agcatcggga cgctgccagt aacccagcat cacctgcggt cctttgacac
    16681 aaagctcacc cggttgaccc ggtggtactt cattatcgtc gtcatccacc agtttggctt
    16741 ccgtcgacgg caccggcaaa ccgatgctac cactatgata atcaatatca tatgggttaa
    16801 cgctgaccag cggcgcacac tcggtaagac catagccttc cagcagatac tgtcctgtca
    16861 gtttcaccca acgctctgcc accacttgct gcactggcat cccaccgcct gcggaaagat
    16921 gcagactgga gaaatccagc tgctggaact ctttattgtt cagcaacgca ttgaacaagg
    16981 tgttaacgcc agtgatagcg gtaaacggat atttcgctaa ctcttttacc aaccctggaa
    17041 tatcgcgcgg gttagtgata agcaggttct gcccacccag ttcgataaac agcaggcagt
    17101 taatggtcag ggcaaaaatg tgatacagcg gcagtgccgt tactaccagt tctttacctg
    17161 gatgcagcaa cggcccatag gtggcgttga cctgttccag gttcgccagc atattgcggt
    17221 gagtcagcat cgcgcctttc gccacacctg tggtgccgcc ggtgtattgc aggaaggcta
    17281 aatcttccgg caccagttcg ggtttgacgt actgcatccg gtagccgtta tgcagtgcgc
    17341 tacgaaatga aatggcatct ggcagatggt atttcggcac caaacgcttg atgtatttaa
    17401 caacgaaatt gactaccgtg ccttttgccg tagatagctg atcgcccata cgggtcagaa
    17461 ttacgtgctg aacggcggtt ttatcaacca ctttttccag tgtgtgagca aagttagaca
    17521 cgataacaat cgccgatgcg ccgctatcgt taagctgatg ctcaagctca cgcggggtat
    17581 acaacgggtt aacgtttacg acgatcatcc cggcacgcaa aatgccaaac agcgccaccg
    17641 gatattgcaa taaattaggc atcatcaacg caacgcgatc gcctttcttc agccccaacc
    17701 cttgttgcaa ataagcggca aacgcgcgac tgcgttcttc cagcttgcgg aaggtcatta
    17761 cctcccccat attcacaaac gcaggctgat cggcgtagcg gacgaccgac tgctcaaaca
    17821 tatctatcag agattgataa cggtcagggt tgatctccgt cggaacatcc gcgggataac
    17881 ggttaagcca aaccttcttc aattcttcac ctctaaaatg cgtgttcgtc gtcatcgcaa
    17941 ccccaagtga taaacatgtt gttaacataa cattaactca tcttaccagc ttgataatta
    18001 gacaacgaaa aggttgcgaa gcgcgtcact atttattttt atagttgccg tatgaacgca
    18061 gaaacagcgg accagccgct gtttcttttt ctttaaaaac aacttgttac tcggttacaa
    18121 ttgtttcaac tcttgcaggg ccaggattat accaacccca tccgccgtaa ccatagggcc
    18181 agccgcgtcc accataaaac catggatcaa ttggctgcgg cggcataatc acctgttgag
    18241 tgatatgcca gcgtttgtaa cctgttacct gcataaccat aaatttatat ggtgcattat
    18301 ttactttgcc atccactgtg ccagtgattg gcccgacaac cgtcaccagt tgtccacgaa
    18361 aatcgaccgg atcaagaaaa ccgttcacat cggcataaat gcgcccgcgt gaaggttctc
    18421 ccaaggttgg tcttgctccg ctgtccagcg gtacggtagc aatttccagg cgggttttcc
    18481 cttgctggtt ttgtaccaca accactttgc cgccaaagcg cgcctcctga ccaacatata
    18541 aatctggcgc attcatcacg cgtaccagat cttgctgtgg tgttggactg ctgcctttaa
    18601 tggcgtccgg cacagtgaca caaccgctca gcattagcgc aaacgtacct gccagtatgc
    18661 ctttgataac attcttttga accgccatga tgcgactcct ttttttctca gggcatactc
    18721 ttaagattca ttctttgccc ggaagtttct tccatgcgac gttgttacgt aaataaaccg
    18781 gttcggcatg ttccaccgcc accgttttac cctcggcaaa catctgacac gcaatcggca
    18841 gcatatcttc cgccgctggc agtaaaactt ctccgtcgcg caaaaccagc ccgctctctt
    18901 tacggagatc cggccaggct tgccagcccg tccctaccgt tacccattcg ccggaaagct
    18961 gttgcattcg ttcatgaacg agttcgggtt tgagtacggc ttcggtttct tcaccgcgcc
    19021 agataccgtt ttcatcacgc tgatattcgg cccagtaaac ttcgcccatt cgcgcgtcaa
    19081 tggctgccag cacgcgggtt gcgccgtttt tgcgccacgc cccctgcgcc atcgtcatta
    19141 gcgtagagac accaatcatc ggtaactccg cgccaagcgc cagcccttgc gcaataccaa
    19201 taccaatgcg cacgccagta aagctaccgg ggccgcgccc gtaagccaga gcgtttatgt
    19261 cagtcaggga agttccgctg gtggtcagga tatcctgcac catcggtaag attcgttgag
    19321 tatgttcacg agggcaaagc tcaaaatgag cgttgacatt accgtcgttc cacagagcga
    19381 cagagcagac ctctgtcgcg gtatcgatag ccagaattcg catgggtctt cgtgcttaga
    19441 tcaataaaaa ggcgcgcatc ataccatact ccgtaacaaa ttacctggag gatggtatcg
    19501 caaggaaacg aaccgcacgg gcaatgtcac gggtgcgtgg cgcgggcggc agactggcga
    19561 gaaacgtcgc gccgtaagga cgcatcacca gccgattgtc acaaattacc agcacgccgc
    19621 gatcgtcggc gtcgcgaatc agtcgcccta ctccctgctt gagagtaatg accgcatccg
    19681 gcagctgcac ttcatcgaac gggtcgccac cgcgcaaacg acaatcttcc atgcgcgctt
    19741 ttaacagtag atcatccggc gaggtaaacg gcaatttgtc gataattacc aatgacaatg
    19801 tatcgccacg cacgtccacc ccttcccaga aactactggt tgccacaaga agcgcgttac
    19861 cggcgctgac aaattgctgc aacagttgcc ctttgctggt ttccccctgc aacaaaacgg
    19921 gaagcgtcat ggtagcgcgg aactgctcgg ccagatcgcg catcatggca tgcgaggtac
    19981 aaagcataaa acaacgaccg ttgttagctt cgatgatcgg tcgcagcatt gccgccagtt
    20041 gccgtgcaca acccggttga ttggtttgcg gcaggttgcg cggcacacag agtaacgcct
    20101 ggcggctgta atcaaacggg cttggcaata gcaacgactc cgcctgttct atcccaagcc
    20161 gcgaggtgaa atgatgcaga tcgtcgttca ccgacagcgt tgctgaggtg aagatccagc
    20221 taccgggctt ttgcgccatt aactctttga atttatccgc cacgctgagc ggcgtgagag
    20281 ccagagtaaa atggcgcgaa gtgcattcgt accagtagct gtagcccggc tgattgatct
    20341 cttttagccg cttcagccgt gtgcgataca acgtggcgcg ctcaaatgcc gcatccagca
    20401 aggcggaacg ccccagtgac agtttcgcca cgtcataaca aagttccagg gtgtcatcga
    20461 gcagtaaaaa tgcccgctga atttgcggat tagctaacag ctcacgcagg ttaccgcgat
    20521 aacctggctc accgagttgc agacgaaaat cctgtgcact ctgggcaaga cgatcagcgc
    20581 acttttgtaa ctgctgggtg tcttttaatt cggtgcggta ggcgatggtg atgtcttttg
    20641 ccaggtcgag cagttgtcga ctggagagtg actgaccaaa atactggctg gcaatgtccg
    20701 gtagctggtg ggcttcgtcg aagatcatga cgtccgcttc agggatcaac tcgccaaatc
    20761 cactctcttt aaccaccata tccgccagaa agagatgatg gtttaccacc accacatcgg
    20821 cgtccatcgc ttttttacgt gctttgacca caaagcaatc tttatacatc gggcagtcgc
    20881 tgccaagaca gttgtcgttg gtgctggtga ccagcggcca cgcctgtgaa tcttccgcca
    20941 cgctgacgca ggtgctgata tcaccatcga ctgtttgatt agaccaggag cgcagcagga
    21001 tcacatcgct taagatttgt accggcagat cgccccccgc cagcgcctgc tgttcgagac
    21061 gttcgaggca gaggtagttt gagcgccctt tcagcagcgc cacgttgccc gtatatttca
    21121 atgcctttga gactgttggc aaatcgcggc tgtagagctg atcctgcaac gcttttgagc
    21181 cggtcgagat aatgactttc tttttcgccc gcagcgcagg agccaggtaa gcgtaggttt
    21241 tgcccgtacc ggttcctgct tccaccacca gcggctggcc tttttctatc gcctgggtga
    21301 cggctaccgc catctgtcgc tgtggttctc gcggcttaaa gcctggtatc gctttcgcca
    21361 gctgaccgtc tggtgcaaaa tcgtccgtca cactaccccc tgttgatttg aacagggatt
    21421 atgtcaggat gagggcgctt tcgccagttg aagtggtgac ggcaacctca cattgtggca
    21481 gtctttgcag cacgaaaatg gaagtataat gaggacaaaa tgatgactat cgttcgtatc
    21541 gatgctgaag cccgctggtc tgatgtagta atccacaaca acacgctcta ctacactggt
    21601 gtaccggaaa acctcgacgc cgatgccttt gagcaaaccg ccaacacgct ggcacagatt
    21661 gacgccgtgc tggaaaaaca gggcagcaat aaatcgagca ttctggatgc caccattttc
    21721 ctggccgata aaaacgactt cgcggcgatg aataaagcgt gggatgcctg ggttgtcgcg
    21781 ggtcatgcgc cagtgcgctg cacggtacaa gcgggtttga tgaacccgaa gtataaagtt
    21841 gaaattaaga ttgtggctgc ggtgtaagct ttatcgaagc aaaataagtc agacgataat
    21901 ttatcgataa tactggtcgg ttttacataa atcgaccaga
//
LOCUS       DAZRUF010000048        21643 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZRUF010000048 DAZRUF010000000
VERSION     DAZRUF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.048"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="ACHXOL_001468"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="ACHXOL_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR6808484.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="ACHXOL_001469"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="ACHXOL_001469"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR6808485.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="ACHXOL_001470"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="ACHXOL_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR6808486.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="ACHXOL_001471"
     CDS             complement(3717..4511)
                     /locus_tag="ACHXOL_001471"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR6808487.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="ACHXOL_001472"
     CDS             4649..4990
                     /locus_tag="ACHXOL_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR6808488.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="ACHXOL_001473"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="ACHXOL_001473"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR6808489.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="ACHXOL_001474"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="ACHXOL_001474"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR6808490.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="ACHXOL_001475"
     CDS             8906..10198
                     /locus_tag="ACHXOL_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR6808491.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10229..10326
                     /locus_tag="ACHXOL_001476"
                     /pseudo
     CDS             10229..10326
                     /locus_tag="ACHXOL_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="ACHXOL_001477"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="ACHXOL_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR6808492.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="ACHXOL_001478"
     CDS             complement(10731..11189)
                     /locus_tag="ACHXOL_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR6808493.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="ACHXOL_001479"
     CDS             complement(11186..12103)
                     /locus_tag="ACHXOL_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR6808494.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="ACHXOL_001480"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="ACHXOL_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR6808495.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="ACHXOL_001481"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="ACHXOL_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR6808496.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="ACHXOL_001482"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="ACHXOL_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR6808497.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="ACHXOL_001483"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="ACHXOL_001483"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR6808498.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="ACHXOL_001484"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="ACHXOL_001484"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR6808499.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /gene="ybbA"
                     /locus_tag="ACHXOL_001485"
     CDS             16063..16749
                     /gene="ybbA"
                     /locus_tag="ACHXOL_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR6808500.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /gene="ybbP"
                     /locus_tag="ACHXOL_001486"
     CDS             16746..19160
                     /gene="ybbP"
                     /locus_tag="ACHXOL_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR6808501.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="ACHXOL_001487"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="ACHXOL_001487"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR6808502.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="ACHXOL_001488"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="ACHXOL_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5014 a         5686 c         5799 g         5144 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtagcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAZRUF010000049        21114 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZRUF010000049 DAZRUF010000000
VERSION     DAZRUF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.049"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHXOL_001489"
     CDS             complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHXOL_001489"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR6808503.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHXOL_001490"
     CDS             complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHXOL_001490"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR6808504.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHXOL_001491"
     CDS             complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHXOL_001491"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR6808505.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHXOL_001492"
     CDS             complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHXOL_001492"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR6808506.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHXOL_001493"
     CDS             complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHXOL_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR6808507.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHXOL_001494"
     CDS             complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHXOL_001494"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR6808508.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLAITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHXOL_001495"
     CDS             complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHXOL_001495"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR6808509.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8450..11212
                     /gene="barA"
                     /locus_tag="ACHXOL_001496"
     CDS             8450..11212
                     /gene="barA"
                     /locus_tag="ACHXOL_001496"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR6808510.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLDLEPELLELLDEMDNVARE
                     ASKILG"
     gene            complement(11444..12784)
                     /gene="gudD"
                     /locus_tag="ACHXOL_001497"
     CDS             complement(11444..12784)
                     /gene="gudD"
                     /locus_tag="ACHXOL_001497"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR6808511.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAATAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12805..14145)
                     /locus_tag="ACHXOL_001498"
     CDS             complement(12805..14145)
                     /locus_tag="ACHXOL_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR6808512.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14147..15499)
                     /gene="gudP"
                     /locus_tag="ACHXOL_001499"
     CDS             complement(14147..15499)
                     /gene="gudP"
                     /locus_tag="ACHXOL_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR6808513.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15934..16383)
                     /locus_tag="ACHXOL_001500"
     CDS             complement(15934..16383)
                     /locus_tag="ACHXOL_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR6808514.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16401..17183)
                     /gene="truC"
                     /locus_tag="ACHXOL_001501"
     CDS             complement(16401..17183)
                     /gene="truC"
                     /locus_tag="ACHXOL_001501"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR6808515.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17183..17512)
                     /locus_tag="ACHXOL_001502"
     CDS             complement(17183..17512)
                     /locus_tag="ACHXOL_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR6808516.1"
                     /translation="MTTHDRVRFQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18134..18679)
                     /gene="syd"
                     /locus_tag="ACHXOL_001503"
     CDS             complement(18134..18679)
                     /gene="syd"
                     /locus_tag="ACHXOL_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR6808517.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18747..19595
                     /gene="queF"
                     /locus_tag="ACHXOL_001504"
     CDS             18747..19595
                     /gene="queF"
                     /locus_tag="ACHXOL_001504"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR6808518.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19707..21071
                     /gene="ppnN"
                     /locus_tag="ACHXOL_001505"
     CDS             19707..21071
                     /gene="ppnN"
                     /locus_tag="ACHXOL_001505"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR6808519.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5147 a         5669 c         5052 g         5246 t
ORIGIN      
        1 ggaggctggc atttttaaat cagataaagt cagtcttatg cctggccttt gatctcttta
       61 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
      121 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
      181 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
      241 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
      301 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
      361 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg
      421 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agatacgatc
      481 ggatactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaaagctttg
      541 ttgccttcgc cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc
      601 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
      661 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact
      721 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
      781 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
      841 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcatg
      901 gagtatttgc ccggagtgcc gttcagttca gcgatgtgct cgtacagcgg catcccttta
      961 gcggcagcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     1021 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     1081 ttagcgtctt tgccaatcag tgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     1141 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     1201 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     1261 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     1321 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     1381 acgcgtaccc aaggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc
     1441 tggaactcgc tggcagcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     1501 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     1561 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     1621 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     1681 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     1741 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     1801 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     1861 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     1921 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     1981 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     2041 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     2101 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     2161 tcaatgtact tgccgaccat accgatggtg acttcactta ccggattcgc ttcttcgaag
     2221 ataacctgtt cccattcgga cagatttgct tccgggcagt ttaagctgaa tcgtttacaa
     2281 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     2341 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     2401 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     2461 gaaagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     2521 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     2581 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     2641 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     2701 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     2761 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     2821 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     2881 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt
     2941 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     3001 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     3061 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     3121 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     3181 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     3241 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     3301 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     3361 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg aatttttcgt
     3421 tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga ttaaccgtgg
     3481 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     3541 cctcgtacat cacctcgtcg atctcttcgt agactttatc aaccaccggg ccaagcgtcg
     3601 tccagtcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcccgc attaaagccg
     3661 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     3721 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     3781 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     3841 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     3901 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     3961 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     4021 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     4081 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     4141 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     4201 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     4261 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     4321 gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     4381 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     4441 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     4501 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttgacca
     4561 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     4621 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     4681 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     4741 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     4801 aatttctgcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4861 tttacccagc acgcggccca gcacctgcag gttgtaaatc tcaatggtca tgtcgatggt
     4921 tgccagttgc tgttttgtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4981 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5041 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5101 cgcatgagag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5161 cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5221 gtggtgcatc aggttgccaa caccttcgac taccacgcga ccgttatctt tactgcggtt
     5281 ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct gctcttcggc
     5341 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5401 gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg gcagcagatg
     5461 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5521 cccagccaga atgtttttgt cacggtcctg tttacggaac caagcgtgaa ttttcgaacg
     5581 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5641 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     5701 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5761 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5821 gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt cgccggaatc
     5881 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5941 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6001 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt tgccacctgg
     6061 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacataatc
     6121 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcatagca
     6181 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcaaaggc
     6241 gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac gaccatacac
     6301 ttccgcttta acgccttcag ctttcatctc agcgcgcaga tgaccaacga actcttcgat
     6361 gtagtgttcg cggtcgagac gccgttcatg cagcagtttg gcaattcgtt tgtattcggt
     6421 tggatggagg taacggaagc agtaatcttc cagttcccat ttcagttgtc cgattccgag
     6481 acggttagcc agcggtgcgt agatgttggt acactctttt gccgccagta cacgttcatc
     6541 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6601 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6661 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6721 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6781 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6841 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6901 cccctgcgtc tgttgcagac aatacgccca agtttcggct aagcactcac acgacttctg
     6961 gctggtaata gccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7021 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7081 gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7141 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7201 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7261 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7321 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7381 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgcc
     7441 ggaacgcctt ctacacccac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7501 cccataccgc agaacaaatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7561 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7621 aaagttaagc gcaacccatt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7681 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7741 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7801 gttgcctgta ccagttcaac atgaccaaga tggcgcataa cttgtaagct gcccaggcat
     7861 gccatgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca ttgtttaacg
     7921 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7981 aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc ggcgatcact
     8041 tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8101 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8161 gtttcgcgtc ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8221 ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg gataaatagc
     8281 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8341 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8401 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8461 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8521 gctgagtatc tttttcgttg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8581 cggagccagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8641 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8701 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8761 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8821 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8881 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgctggagct
     8941 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca gcgtgatgat
     9001 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9061 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9121 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9181 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9241 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc tggcgaaaaa
     9301 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9361 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9421 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9481 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9541 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9601 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9661 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9721 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9781 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9841 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccggtct
     9901 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9961 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10021 tattatcgaa gggccatcca cccagtgcct cgccggtaag cgcctggcct atgtcgaacc
    10081 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10141 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10201 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10261 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg cagaaaaact
    10321 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10381 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10441 caagctggca atgacagtca tggcagttga tgacaacccc gctaacctga aacttatcgg
    10501 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcggt
    10561 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgcctgacat
    10621 ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac aaacgccggt
    10681 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10741 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10801 caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10861 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10921 tctagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10981 tgaggagcag ctggttggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11041 tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac tgatcgaaca
    11101 acagctacgt agcggtacta aagaagaaga tttggatttg gagccggagc tgctggaact
    11161 gttggacgag atggataatg tcgcgcgcga agccagcaaa attctcgggt aacggacgtc
    11221 ttgtcgagta gcaatcggct ttttatgacg ctggtttcag attggtgaca aagtgcctgt
    11281 gtttatgccg gatgcggcgt taacgcctta tccggcctac aaaaccaatt aaattcaatg
    11341 aattaaaaaa tgatgtagac ctgatacata gcacaacagg ctattttgcg tttagcatca
    11401 gtctcaagcc ggctccagat agagccggtt ttggttttct gtcttaacgc accatgcacg
    11461 ggcgcttgtt atcgaacgtc cagccaggaa tcagatactg cattcccatc gcatcgtcac
    11521 gcgcgccaag cccgtgtttc tgatacagct catgggcttt catcacttga tccatatcga
    11581 tttctacacc cagccccggt ttttccggca cctgaaccag accgcctttg atctcaaacg
    11641 gttctttggt caagcgctga ttgccttcct gccaaatcca gtgcgtatca atcgcggtga
    11701 ttttacccgg tgcagtggcg gcaacatggg taaacatcgc cagggaaata tcgaagtggt
    11761 tgtttgagtg tgaaccccag gtcaggccaa attcatggca catttgcgcc acacgtaccg
    11821 aaccttgcat agtccagaaa tgcggatccg ccagcgggat atcaacggat tgtaacgaga
    11881 gcgtatggcc catctgccgc cagtcggtgg cgatcatatt ggttgcagtc ggcaggccag
    11941 tcgcgcgacg gaactctgcc atcacttcac gcccggagaa accttgctcc gcaccatacg
    12001 gatcttctgc ataagccagc gaacctttca ggtatttacc aattttaatt gcttcgttca
    12061 gcgaccaggc accgttagga tcgagcgtaa tacgcgcctg cgggaagcgt tgcgccagtg
    12121 ccacaataga ctcggcttct tcttccccgg ccagtacacc gcctttcagt ttgaaatcgt
    12181 tgaagccata tttttcatac gccgcttccg ctaggcgcac caccgcatcc ggcgtcatcg
    12241 cttcttcatg acgcaggcga taccagtcgc atgagtcatc cggctggctt tgatacggca
    12301 gaggtgtggc tttgcgatta ccgacgaaga acagataacc gagcatttcg acttcgctac
    12361 gctgttgacc atcgcccagc agcgatgcca cgtttacccc cagatgctgc cccagcagat
    12421 ccagcattgc cgcttctatc ccggtaacta catgaatagt ggtacgcaaa tcaaatgtct
    12481 gcaaaccgcg cccaccagca tcacgatcgg caaaagtatt acgcaccagc gtcagaacgt
    12541 ttttgtattc acccagcgtt ttacctacca ccagcggaat cgcatcttcc agcgttttac
    12601 ggattttctc gccgccggga atttccccta cgccagtgtg accggaatta tctttgataa
    12661 tcacaatatt acgcgtaaag aacggtgcgt gtgccccact cagattcatc agcatactgt
    12721 catgacccgc caccgggatg acctgcattt cagtaacaac agacgtcgta aattgagaac
    12781 tcatatttat gtcctttttc agaatcaatg acggccgaaa acgggacgtt tacggtcaaa
    12841 ggtccagccg gggatcaggt actgcatcgg acctgcgtcg ttacgcgcac cgccaggcag
    12901 acgtttatag gcctcatgtg ccttttgtac ctgttcccag tccagttcca cgcccagacc
    12961 gggcgcatca ggaacggcaa tttttccgtt tttaatctcc agcggatttt tggtcaggcg
    13021 acaatcgccc tcctgccaaa tccagtgggt atcgatagcg gtaggattac ccggtgccgc
    13081 cgcgcccaca tgggtaaaca tcgccagaga gatatcgaaa tggttattag aatggcagcc
    13141 ccaggtcagc ccccagtcgt cgcaaagctg cgccacacgg actgcaccgg aaagcgtcca
    13201 gaagtgcgga tcggcaagtg gaatatctac cgcattgagc atcaccgcat gacccatttc
    13261 gcgccagttg gtggcgatca tgttagtcgc gacgggcaag ccggtcgccc gccgaaactc
    13321 cgccataact tcacgaccgg agaaaccctg ttctgcgccg catggatctt cggcataggt
    13381 aagaacatca ttcagccctt tgcatagaga aatggcttca tcaagtagcc aggcaccgtt
    13441 gggatcaacg gtaatccgcg catcagggaa gcgtttcttc aatgcacgaa cagtgtcgat
    13501 ttcttgctcg ccaggtaaca ccccgccctt aagtttgaaa tctttaaagc catagcgatc
    13561 ctgtgaggct tccgccagac gcacaacggc ttcgctgttc atcgctttct gatggcgcaa
    13621 ctgataccac tcatggttgc ccggcgtatt ttccagataa ggaagatcgg ttttggtccg
    13681 atcaccgata taaaacagat aaccgaggac ggtaatagtc tcgcgttgct tgcctggccc
    13741 caacagttcg cagaccggaa cattcagcgc cttacctagc aggtcaagca aggcggcttc
    13801 cagcgccgcc acggcattaa cgcgcaattc aaaagtccag gcacctttgc cgaaggtatc
    13861 aaaatcggct gcctgattac ctttatgcac ctgctgaacc actttattca ggcgcgcaac
    13921 ttcctggccc agaaccatcg gaatagcatc gaccagtgtt tgataaatca cctctccgcc
    13981 cggcgcttca ccaatgccgg tatgcccggc gttatcggtg agtaccacaa tattgcgggt
    14041 gaaatatgcg ttatgtgccc caccaatatt aagcaacatg ctatcgtgcc cggcgaccgg
    14101 aatgactttc atatcagtaa taacaggact ggattgtgtc gtcatcttat tgccccgcga
    14161 caggtttcag ctcgatacgc ttgatatcgc ccaccagcac caggtagctc agtaccgcga
    14221 ttaaggcatg aacaccaaca taaatcagcg ccccattaaa ggagccagtc gtgccaacga
    14281 tataaccaat tgcgattggc gtgacgatgc cagaaatgtt accgaacatg ttgaacaggc
    14341 caccggaaag accgctgatc tcttttggcg cggtatctgc cattactgcc caacccagcg
    14401 ccccgatgcc cttaccgaag aaggccagcg ccataaagcc gatgatcatc cactcaacgt
    14461 tgacgtagtt gcagaacacc atcaccatcg acagcaacat gcccattacg atcggtgttt
    14521 tacgcgcaat gttcagcgat cccgtgcgac gcatcagcca gtcggaaata atcccaccca
    14581 gcacaccgcc gataaaaccg caaaccgccg gaacggaagc cacaaagccc gctttcagaa
    14641 tcgacatccc acgcgcctgc accagataaa ccggaaacca ggtaatgaaa aagtaagtca
    14701 gggcgttgat acagtactga ccgatataaa cgccgatcat catccgtgac ccgagcagtt
    14761 gtttgatctg cccccacttc acgctgaacg gcactttaac tttggtgttt tgctgatcca
    14821 tattgatcag cgcgccaccc gcggcgatgt actccagctc tttctgattt acccccggat
    14881 gttgatttgg ctcgtggatg actttcaacc agataaagct gatgacaatc cccagaccgc
    14941 ccataaagaa gaagacgtgt gaccagccca cttcatgcgt cagccagccc ataatcggcg
    15001 cgaagatcac cgttgcgaag tattgagcgg agttaaaaat cgacaccgcc gttccccttt
    15061 cctgcgccgg aaaccaggcc gcaacaatgc gactgttgcc ggggaaagat ggcgcttcag
    15121 caagcccgac caggaagcgc agagtaaaca gggcaacgat aatgccgaat ccactaaaga
    15181 tatcgacgaa gccttgcagc aaggtaaaca tcgaccagat aaagatcgac cagaagtaga
    15241 cgcgttttga accaaaacga tccagcaacc aaccaccagg gatctgcccg ataacataag
    15301 cccatgagaa ggcagagaac acatagccca ttcccacggg atcaaggccg atatctttgg
    15361 ccatttccga accggcaata gaaagcgtag cgcggtcgcc gtagttgaag gatgtgacga
    15421 taaacaacat caccactatc cagtaacgag cattcgtgcg tttttccaca ctgctcgcag
    15481 cctgacttaa agaactcatt gttgcactcc tgaaaattcg cgttagccac gctcactctg
    15541 gactgcgaca tagccaggaa atcagaggtg acgtagggtg ttttttgccg tttttatagg
    15601 tcgttcgccg aatacggcgc gtgtttatat ctggcaatag cagtataaaa agcgcgccat
    15661 agcggctcac cgtgcaacaa cacaacatta atgcgttcaa tgaggcccga ttttggcatt
    15721 agccctggac ggtggaatcc acttcacgga aatgaaaaca agaacaagaa aggaagggtt
    15781 aaaacgaaga aataaagaag agtatgaaat ggatcgcttg actccaggca aacgccagta
    15841 aaaatccgcg ctatgaagca gtttttactg gcatttgcct gaaaagattc gattcagcac
    15901 cgctaaaacg acatttaccg ctcgctgaac atatcaggac aacagcgtgc cccactgttc
    15961 gacccacgga tttgattccg tttccggttc cgggttctca ctggcgtcaa tcaacagcat
    16021 ttcgccaacc cgctgagcgc tctgttcctg caacaaggca tcgaactgtt tgccgccatt
    16081 gcagaaattt acataactgc tgtcgccgag cgcaatcacg ccataacgca gattcggctg
    16141 gaagcccaga ctatctttga ttccctgaaa gagcggcaca atgctatcag gaaggtcgcc
    16201 ctgcccggtc gtggacgtaa ccaccagaac atacttatcc tgataaggca gccagtcgct
    16261 taattcagga tcttcaaata ccgttgcttt gtggccctgt gcggtcagaa ttgcttctgc
    16321 ttcttccgcc actaacagcg aatttccgta catggtgccg acaaaaatac caatttccgc
    16381 cataccgttt tctccctgga ttaggaactt atctctccat cctgacccga tggcgcactg
    16441 aactcaaccc tttcattttc aggaagcaga ccgcgccagc caaattgtga taacgcctgc
    16501 atccaggtgt cgtccaggcc cgcgtgaata gtcagcggct cgccagtaaa aggatgcgtc
    16561 aatgacaact gactggcatg tagcattaac cgctggaggc caaaatgctc agcaccgctg
    16621 cgattctggc gtaaatcgcc atgtttgcta tcgccaataa tcggatgacg caaatgggca
    16681 agatgtcggc gaagctgatg tttgcgtccg gttttcggct ccagttccac caggccgtag
    16741 cgcgtggtcg ggtaacgtcc ggtcgctacc ggcatttcta cggtcgccag accgcgataa
    16801 tgcgtcactg ctggctgcgg gcctttatct tcgcgggcaa atttatcagc gattttgtcc
    16861 agttcttcca ccagtggata atccagcacc gcttcttcca tcaaccagcc gcgcacaatc
    16921 gcatggtaac gtttctggat ttggtgctgt tcaaactgtt gtgccagcag ccgtccggcc
    16981 tcgctggata atcccatcaa caacacacca gaagtgggtc ggtccagacg atgagcagta
    17041 aaaacatgct gtcctatctg gtcacgcacg gtttgcatga ccactacttt ctcgtcgcga
    17101 tccagccagc tacggtgaac cagccagccg gagggtttat ttaccgcaac cagccattca
    17161 tcctgataga gtatttccag cattagctcg catcatccgc aaaaagagca tccagttttt
    17221 ccaactcagc cagaataagc gcgcgttgcg gatggtccgt cgccagcgcc atttcataat
    17281 agggtgcaac agcaaaagcg cccggtaacg gctggttgtt atttaacaaa tcgtgcattc
    17341 gcgggatcag cacccactgc aaccactcca gtggttccat ggtgtccata aagaacggtt
    17401 gggtactatt aaattgatgc ggctggggtt catcgtttcg ccagtgctga tgttcacgca
    17461 gtaacgcttc aagcgtctgc aactggaaac gaacgcggtc atgagtggtc ataaagcaac
    17521 ttcaataaga aaaactgccg cgaaggatag caggaataaa aaaagggagc actgtattca
    17581 cagtgctccc ggttcgtttc gcagcattcc agctactttt gttgctccct gctcatcctt
    17641 gacaactttt cctctggcct tgcggccaat cgttcatcct gaactattgc ttcctgctca
    17701 caccaccccg atgtgatact tcatcctgaa gtgtccctgg ccttcctgac ccaccgaatc
    17761 atcctgaccg gttctcattc tccatcctgg aggtgtcctt taacgcgtcc tgcgtcatcc
    17821 tcttcgcttc atccagaagc ctttccctga aacaccatcc tggtgtgtcc tgcagaagtg
    17881 tcatcatcct gatgttcact tcgttgtctg actccctgtc gacgaagata gaatcgtctt
    17941 tttcgcgaag tcttacaagg cgcttacaga caatgcctta aggaaaattt catatgaaag
    18001 ttaagaatta acaatcaaca ttttgatttt aaatgaaaat aacgattctt atctttttaa
    18061 gtattcgctt atttcctggc gatctcctac agggtctgta agagatctct cacaaccaga
    18121 tggggtattt ggattacaga agaggcttaa gttgattaag gaattccgca agatttgagg
    18181 ctaaatgcgt ccgtttacgt gttcccagcg tctctttgca cacttcaccg gagagattac
    18241 acaccgagat gacctctaac tcttcttcca gcgtcgcgat aaatagcgtg ggcggcagtt
    18301 ttagcctttt ctgagtcacc agatgaccaa tcagattttc ctgcacccga cggaagtcgt
    18361 cttcactcca ggtttgcagc aatgtcagtt tgatatcgcc aaactgcgcg tgcatatccc
    18421 cggcaaactg agtcgtataa aaggtatgaa ttgtgggttg tatcacaata tcaaaagcgc
    18481 gttcaaccgc gtttacattt tgttccccgg taaacggctg gggttgccag tatacggcat
    18541 cttcggtagt tgaaataatg cacggtgaag gaacaccgta cagttcctca cttaacggcc
    18601 agcttttatg ctcttcatgc cacgcatcgc agtagcgtgc agtaaaatct ttcagggctt
    18661 gtgcggtcaa atcgtccact aatttctctc ttcacttaaa ccagatacac ttgtcgttta
    18721 gtttatctgg tttatgacgg tgaaacatgt cttcttatgc aaaccatcag gcacttgcgg
    18781 gcctgactct tggaaaatca accgattacc gggataccta tgacgccagc ctactgcaag
    18841 gcgttccacg cagcctgaat cgcgacccgc tgggtctgaa agcggataac ctgccttttc
    18901 acggtacgga tatctggacg ctgtatgaac tttcctggct gaatgcgaaa ggtttgccgc
    18961 aggtcgctgt cggtcatgtt gaacttgatt acaccagcgt aaatctgatt gagtcgaaga
    19021 gttttaagct ctatctcaac agttttaacc agacgcgttt taataactgg gatgaggtgc
    19081 gccagacgct ggagcgcgac ttaagcactt gcgctcaggg taagattagc gtggcgttat
    19141 atcgtcttga tgaactggaa ggccagccga taggtcattt taatggcact tgcattgatg
    19201 accaggatat cactatcgat aactatgaat tcactactga ctatctggag aatgccacct
    19261 gtggtgaaaa agtagtggaa gagacgcttg tcagccacct gctgaaatca aactgcctga
    19321 tcacccatca accagattgg ggttcgatcc aaattcagta tcgtggacgc caaattgaca
    19381 gagaaaaact gctgcgttac ctggtctcat tccgtcatca caacgagttc cacgaacagt
    19441 gcgtggaacg catctttaat gacctgttac gcttctgcca gccagaaaaa ttgagcgttt
    19501 acgcacgtta tacccgtcgt ggcggtctgg acattaaccc gtggcgcagt aatagcgatt
    19561 ttgtcccatc gaccacaaga ctggttcggc aataaatttt ttctcaattt tgcgtgctgg
    19621 attcacgcag aaggttgtga aaggacatca ggcagggcta ttgtaatcaa agggaatgac
    19681 gatattcgtc ccataaggag tttttcttga ttacacatat tagcccgctt ggctccatgg
    19741 atatgttgtc gcagctggaa gtggatatgc ttaaacgcac cgccagcagc gacctctatc
    19801 aactgtttcg caactgttca cttgccgtac tgaactccgg tagtttgacc gataacagca
    19861 aagaattgct gtctcgtttt gaaaatttcg atattaacgt cctgcgccgt gaacgcggcg
    19921 taaagctgga actgattaat cccccggaag aggcttttgt cgatgggcga attattcgcg
    19981 cgttgcaggc caacttgttc gcggttctgc gtgacattct cttcgtttac gggcaaatcc
    20041 ataacaccgt tcgttttccc aacctgaatc tcgacaactc cgtccacatc actaacctgg
    20101 tcttttccat cttgcgtaac gctcgcgcac tgcatgtcgg tgaagcgcca aatatggtgg
    20161 tctgctgggg cggacactca attaacgaaa acgagtattt gtatgcccgt cgcgtcggga
    20221 atcagctggg cctgcgtgag ctgaatatct gcaccggctg tggtccggga gcgatggaag
    20281 cgccgatgaa aggtgctgcg gtcggacacg cacagcagcg ttacaaagac agtcgtttta
    20341 ttggtatgac agagccgtcg attatcgccg ctgaaccgcc taacccgctg gtcaacgaat
    20401 tgatcatcat gccagatatc gaaaaacgtc tggaagcgtt tgtccgtatc gctcacggca
    20461 tcattatctt ccctggcggt gtgggtacgg cagaagagtt gctgtatttg ctgggaattt
    20521 taatgaaccc ggccaacaaa gatcaggttt taccattgtt cctcaccggc ccgaaagaga
    20581 gcgccgacta cttccgcgta ctggacgagt ttgtcgtgca tacgttgggt gaaaacgcgc
    20641 gccgccatta ccgcatcatc attgatgacg ccgctgaagt cgctcgtcag atgaaaaaat
    20701 cgatgccgct ggtgaaagaa aatcgccgcg acacaggcga tgcctacagc tttaactggt
    20761 caatgcgcat tgcgccagat ttgcaaatgc cgtttgagcc gtctcacgag aatatggcta
    20821 atctgaagct ttacccggat caacctgttg aagtgctggc tgccgatctg cgccgtgcgt
    20881 tctccggtat tgtggcgggt aacgtaaaag aagtcggtat tcgcgccatt gaagagtttg
    20941 gtccttacaa aatcaacggc gataaagaga ttatgcgtcg tatggacgac ctgctacagg
    21001 gttttgttgc ccagcatcgt atgaagttgc caggctcagc ctacatccct tgctacgaaa
    21061 tctgcacgta atctccctcc cccggtgacg tttgttgccg ggttttctcg tttt
//
LOCUS       DAZRUF010000050        21008 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZRUF010000050 DAZRUF010000000
VERSION     DAZRUF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21008
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.050"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..173)
                     /locus_tag="ACHXOL_001506"
     CDS             complement(<1..173)
                     /locus_tag="ACHXOL_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR6808520.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            345..1565
                     /gene="coaBC"
                     /locus_tag="ACHXOL_001507"
     CDS             345..1565
                     /gene="coaBC"
                     /locus_tag="ACHXOL_001507"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR6808521.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1543..2001
                     /gene="dut"
                     /locus_tag="ACHXOL_001508"
     CDS             1543..2001
                     /gene="dut"
                     /locus_tag="ACHXOL_001508"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR6808522.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2108..2704
                     /gene="slmA"
                     /locus_tag="ACHXOL_001509"
     CDS             2108..2704
                     /gene="slmA"
                     /locus_tag="ACHXOL_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR6808523.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2741..3382)
                     /gene="pyrE"
                     /locus_tag="ACHXOL_001510"
     CDS             complement(2741..3382)
                     /gene="pyrE"
                     /locus_tag="ACHXOL_001510"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR6808524.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3448..4164)
                     /gene="rph"
                     /locus_tag="ACHXOL_001511"
     CDS             complement(3448..4164)
                     /gene="rph"
                     /locus_tag="ACHXOL_001511"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR6808525.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4291..5154
                     /locus_tag="ACHXOL_001512"
     CDS             4291..5154
                     /locus_tag="ACHXOL_001512"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR6808526.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5376..6200
                     /gene="dinD"
                     /locus_tag="ACHXOL_001513"
     CDS             5376..6200
                     /gene="dinD"
                     /locus_tag="ACHXOL_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR6808527.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6492..7109
                     /locus_tag="ACHXOL_001514"
     CDS             6492..7109
                     /locus_tag="ACHXOL_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR6808528.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7106..8788)
                     /gene="ligB"
                     /locus_tag="ACHXOL_001515"
     CDS             complement(7106..8788)
                     /gene="ligB"
                     /locus_tag="ACHXOL_001515"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR6808529.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9046..9669
                     /gene="gmk"
                     /locus_tag="ACHXOL_001516"
     CDS             9046..9669
                     /gene="gmk"
                     /locus_tag="ACHXOL_001516"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR6808530.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9724..9999
                     /gene="rpoZ"
                     /locus_tag="ACHXOL_001517"
     CDS             9724..9999
                     /gene="rpoZ"
                     /locus_tag="ACHXOL_001517"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR6808531.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10018..12126
                     /gene="spoT"
                     /locus_tag="ACHXOL_001518"
     CDS             10018..12126
                     /gene="spoT"
                     /locus_tag="ACHXOL_001518"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR6808532.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12133..12822
                     /gene="trmH"
                     /locus_tag="ACHXOL_001519"
     CDS             12133..12822
                     /gene="trmH"
                     /locus_tag="ACHXOL_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR6808533.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12828..14909
                     /gene="recG"
                     /locus_tag="ACHXOL_001520"
     CDS             12828..14909
                     /gene="recG"
                     /locus_tag="ACHXOL_001520"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR6808534.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15078..16283)
                     /gene="gltS"
                     /locus_tag="ACHXOL_001521"
     CDS             complement(15078..16283)
                     /gene="gltS"
                     /locus_tag="ACHXOL_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR6808535.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16563..17954
                     /gene="xanP"
                     /locus_tag="ACHXOL_001522"
     CDS             16563..17954
                     /gene="xanP"
                     /locus_tag="ACHXOL_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR6808536.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18075..19784
                     /locus_tag="ACHXOL_001523"
     CDS             18075..19784
                     /locus_tag="ACHXOL_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR6808537.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKHQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19836..>21008)
                     /gene="yicI"
                     /locus_tag="ACHXOL_001524"
     CDS             complement(19836..>21008)
                     /gene="yicI"
                     /locus_tag="ACHXOL_001524"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR6808538.1"
                     /translation="GLAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQ
                     WFDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCY
                     ANYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGS
                     KSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPA
                     CDYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLS
                     LPVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKA
                     TRTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5085 a         5720 c         5386 g         4817 t
ORIGIN      
        1 aagccataaa gagagccgaa attctccagc atctcttttg ccagggttaa tacatcttta
       61 ccgcgcgttc cggtacgcag aaatagcgcc agcagctcga catccgttaa ggcgctaata
      121 ccaaacttca gcattttttc gcgcggcatc aacagctgtg cattgttttt caccttcacc
      181 tcctttgtgg tgctcgcatc ctgtcatagc cgtttcgtta aatcgacggc cagttttcaa
      241 tcctggaaag cgcctcgcaa agtgaatctc aggtgaaggc acgacaacaa aagtattgtg
      301 ataaaatcgc caacttctgg tgtcacccta caggaaaaat catcatgagc ctggccggta
      361 aaaaaatcgt tctcggcgtt agcggcggta ttgctgccta taaaacccct gaactggtgc
      421 gtcgtttgcg cgatcgcggg gccgacgtcc gcgtagccat gaccgaagcg gcaaaagcct
      481 ttatcacccc acttagcttg caggcggttt ctggttatcc cgtttccgac agtctgctgg
      541 acccggcagc cgaagccgct atgggccata ttgagctggg taaatgggct gatttagtga
      601 ttctcgcccc tgccacggca gatttgattg cccgtgttgc tgccggaatg gcgaatgacc
      661 tggtatcgac gatttgtctg gctacacctg cgcctgtagc cgtgctcccc gccatgaacc
      721 agcagatgta ccgtgccgct gccacgcagc ataatttaga ggtgcttgct tcccgtggtt
      781 tgctcatctg ggggccagac agtggcagtc aggcttgtgg tgatatcggt cctgggcgaa
      841 tgctcgatcc gttaaccatt gtggatatgg cggtagcgca tttttcgccc gtcaacgacc
      901 tgaaacatct gaacattatg attaccgccg gcccgacgcg tgaaccgctc gatccggtgc
      961 gttatatctc taatcacagc tccggcaaga tgggttttgc tatcgccgcc gccgctgccc
     1021 gtcgtggcgc gaacgtcacg ctggtatcag gcccggtttc gctaccgacg ccaccgtttg
     1081 ttaaacgtgt tgatgtgatg accgcgctgg aaatggaagt cgccgtgaat gcttctgtac
     1141 agcagcaaaa tatttttatc ggctgcgccg ccgtggcgga ttatcgcgca gctaccgtgg
     1201 ccccagagaa aatcaaaaag caggccacgc agggtgatga attaacaata aaaatggtta
     1261 aaaaccccga tatcgtcgca ggcgttgccg cactaaaaga ccatcgaccc tacgtcgttg
     1321 gatttgccgc cgaaacaaat aatgtggaag aatacgcccg gcaaaaacgt atccgtaaaa
     1381 accttgatct gatctgcgcg aacgatgttt cccagccaac tcaaggattt aacagcgaca
     1441 acaacgcatt acaccttttc tggcaggacg gagataaagt cttaccgctt gagcgcaaag
     1501 agctccttgg ccaattatta ctcgacgaga tcgtgacccg ttatgatgaa aaaaatcgac
     1561 gttaagattc tggacccgcg cgttgggaag gaatttccgc tcccgactta tgccacctct
     1621 ggctctgccg gacttgacct gcgtgcctgt ctcgacgacg ccgtagaact ggctccgggt
     1681 gacactacgc tggttccgac cgggctggcg attcatattg ccgatccttc actggcggca
     1741 atgatgctgc cgcgctccgg attgggacat aagcacggta tcgtgcttgg taacctggta
     1801 ggattgatcg attctgacta tcagggccag ttgatgattt ccgtgtggaa ccgtggtcag
     1861 gacagcttca ccattcaacc tggcgaacgc atcgcccaga tgatttttgt tccggtagta
     1921 caggctgaat ttaatctggt ggaagatttc gacgccaccg accgcggtga aggcggcttt
     1981 ggtcactctg gtcgtcagta acacatacgc atccgaataa cgtcataaca tagccgcaaa
     2041 catttcgttt gcggtcatag cgtgggtgcc gcctggcaag tgcttatttt caggggtatt
     2101 ttgtaacatg gcagaaaaac aaactgcgaa aaggaaccgt cgcgaggaaa tacttcagtc
     2161 tctggcgctg atgctggaat ccagcgatgg aagccaacgt atcacgacgg caaaactggc
     2221 cgcctctgtc ggcgtttccg aagcggcact gtatcgccac ttccccagta agacccgcat
     2281 gttcgatagt ctgattgagt ttatcgaaga tagcctgatt actcgcatca acctgattct
     2341 gaaagatgag aaagacacca cagcgcgcct gcgtctgatt gtgttgctgc ttctcggttt
     2401 tggtgagcgt aatcctggcc tgacccgcat cctcactggt catgcgctaa tgtttgaaca
     2461 ggatcgcctg caagggcgca tcaaccagct gttcgagcgt attgaagcgc agctgcgcca
     2521 ggtattgcgt gaaaagagaa tgcgtgaggg tgaaggttac accaccgatg aaaccctgct
     2581 ggcaagccag atcctggcct tctgtgaagg tatgctgtca cgttttgtcc gcagcgaatt
     2641 taaataccgc ccgacggatg attttgacgc ccgctggccg ctaattgccg cgcagttgca
     2701 gtaatatgac gccggatgac ttttcatccg gcgagtttct ttaaacgcca aactcttcgc
     2761 gataggcctt aaccgccgcc agatgttccg ccatttccgg cttctcttcc aggtaagcaa
     2821 tcaggtcttt cagggtgatg atagagatca ctttgcagtt gtaatcacgc tcaacttcct
     2881 gaatcgccga aatctcgctg cgcccccgtt cctgacgatc gagcgaaatc aacacgccag
     2941 caagcgtcgc gccattggcc tgaataattt ccatcgactc acgaatcgcc gttccggcgg
     3001 tgatcacatc atctaccagc attacgcgtc cttgtaacgc gctaccaacc agattgccgc
     3061 cttcaccgtg gttttttgcc tctttgcggt taaagcagta cggcaggtcc aggtcatgat
     3121 gctccgccag cgccacggcg gttgtggtgg caatcgggat ccctttgtaa gcagggccaa
     3181 acagcagatc gaactcaatg ccggaatcca ccaatgcttc agcgtaaaaa cggcctaaca
     3241 gtgccaggtc gcgcccggta ttaaacagcc cggcgttgaa gaaatagggg cttttacgcc
     3301 cggatttcag cgtaaactcg ccaaacttta acacctgctt gctaagcgca aattcaataa
     3361 actggcgctg atatggtttc atgccttcgc tcctcatctt acttttctac agacaaaaaa
     3421 aaggcgactc atcagtcgcc ttaaaaatca gtttgccagc gccgccttct gcgtcgctac
     3481 aatggattcg attccccctc gggccagagc caacaaggtg agtaattctt cgtgggtgaa
     3541 cggttcgcct tctgccgtcc cctgcacttc aatgatgcgc ccgtcttcgg tcatcactac
     3601 gttcatgtcg gtctctgcgg cagagtcttc aacgtattcc agatcgcaaa ccgcttcgcc
     3661 gttcacaatc ccgacagaaa ccgcggctac catccctttc atcggattgg ttttcagctt
     3721 accgttttcc accagcttct gtagcgcatc tgccagcgcc acgcaggcac ccgtaatcga
     3781 agcggtacga gtgccaccat cagcctgaag cacgtcgcag tccagcgtaa tggtgaactc
     3841 acccagcgct ttcaaatcta ctgccgcgcg aagcgcacgg gcgatcagac gctggatttc
     3901 cattgtgcgt ccaccctgct tacctttcgc cgcttcacga gcgttacggg tgtgggtaga
     3961 acgcggcagc ataccgtact ctgcggtgat ccagccctgg ccctgacctt tcaggaagcg
     4021 cggcacgcct tcttcaatag aggcggtaca caacactttg gtatcgccaa attcgaccag
     4081 caccgagcct tctgcatgtt ttgtatagtt acgagtcagg gtaacgggac gcacctgatt
     4141 attgctacgg cctgctggac gcatattgaa atctccggct tgaaacaaat gtggctgcgc
     4201 attatacgga cttccggagg ttattcctat cctgacaagg catcgatggc tataatcctt
     4261 ccacctctcc ttttataaac aggaacgtct atgatccgca gtatgaccgc ctacgcccgg
     4321 cgtgaaatca agggtgaatg ggggagcgca acctgggaaa tgcgctcggt aaaccagcgt
     4381 tatctggaaa cttactttcg tctgccggag cagttccgta gccttgaacc tgtcgttcgc
     4441 gagcgtattc gttctcgcct gacgcgcggt aaagtggaat gtaccctgcg ctatgagcca
     4501 gatgttagcg cgcaaggtga gctgatcctc aacgaaaaac tggctaaaca gctggtaact
     4561 gccgcgaact gggtaaaaat gcagagtgac gaaggggaaa tcaacccggt tgatattctg
     4621 cgctggccgg gcgtaatggc agcccaggag caggatctcg acgcgattgc cgctgaaatt
     4681 ctcgcggcgt tggatggtac cctggacgac tttatcgtcg cacgtgaaac cgaaggtcag
     4741 gcgctgaaag cgttgatcga gcagcgtctg gaaggcgtca ccgccgaagt ggtcaaagtc
     4801 cgcgcccata tgccggaaat cctgcaatgg cagcgtgagc gtctggtcgc gaagctggaa
     4861 gatgctcaag tgcaactgga aaacaaccgc ctggagcagg agttggttct gctggcacaa
     4921 cgaattgatg ttgccgaaga actggaccgc ctggaagcgc atgtcaaaga gacctacaac
     4981 attctgaaga aaaaagaagc cgttggtcgt cgtctggact ttatgatgca agagttcaac
     5041 cgcgagtcga acactcttgc gtcgaaatct atcaatgccg aagtgacgaa ttctgccatc
     5101 gagctgaaag tgttgattga gcagatgcgc gagcagattc agaatattga gtaactcgta
     5161 acactaattc ttacctttca gccttcacaa atgccgccca ctcaaacaga gcggcatttt
     5221 tcttccccgc aacattcaat tctgttttgc gtacctgctc cagattttgc gatgtttttt
     5281 tgcgcagcgc agtgagaacg tgagatactc acaactgtat ataaatacag ttatagactt
     5341 actttctttg caatcgatat cacatggagt gggcaatgaa cgaacatcat caaccttttg
     5401 aagaaatacg gcattatggc actgaagggc aagaattttg gtcagcgaga gaattggcac
     5461 cgttactgga ttatcgggac tggcgcaatt ttcaaaaggt actggcccgc gcgactcaag
     5521 cgtgcgaagc cagcaatcaa gctgcatctg accatttcgt tgaaaccacc aaaatggttg
     5581 tattaggttc aggggctcaa cgagaacttg aagatgttca tctctctcgc tatgcctgtt
     5641 acctggtagt acaaaacggc gaccctgcga aaccggttat tgcggcaggg caaacttatt
     5701 ttgctatcca gacccgacgg caggagctgg ctgacgatga agctttcagg caacttcgtg
     5761 aagacgaaaa gcgtcttttt ctgagaaatg agttgaaaga acataacaaa caattagttg
     5821 aggcagcgca gcaagctggg gtagcgacag ctatcgattt cgccatattt cagaatcatg
     5881 gttaccgggg gctgtatggt ggattagatc agaaagccat ccatcagcgg aaggggctga
     5941 aaaagagtca gaagatcctg gatcatatgg gttcaacaga actggcggct aatctctttc
     6001 gagctaccca aacagaagaa aaactcaagc gggatggcgt aaattcaaaa cagcaagcta
     6061 ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac cattcaggaa cttggcggaa
     6121 ccatgcctga ggagttgccg accccgcagg tcagcatcaa tcagttggaa aacagtgtaa
     6181 aaattacaga gaagaaatag cagaaactaa aaactctttt tcttaattga gatacccgat
     6241 gcgtaagcca aagtccaggt gcagtgaaca caatggctaa acattgcacc tttcttttcc
     6301 acccctcaat tttaacctat tttttcttat gcattttctc agacaagaag tcagaagaat
     6361 gcatctacac tacagaaaat agcgatttca cataactaca agttatcaat ttcctctcac
     6421 cttaaaaaat ctcaatcgtg acaatacgca caaatcgcta ccctgccaga cagattttta
     6481 gggagagaac catgctgtta cacattttgt atttggttgg cattactgcc gaagccatga
     6541 cgggggcgct ggcggccgga cgacggcgca tggatacatt tggcgtaatt attattgcga
     6601 ccgccaccgc aattggcgga gggtcagtgc gcgatattct gctgggccac tatccgctcg
     6661 gctgggtcaa acacccggaa tatgtgatta tcgtcgctac cgccgcagta ctcaccacta
     6721 tcgttgctcc tgtaatgcct tacctgcgca aagtgtttct ggtgctcgat gcgctcggac
     6781 tggtggtctt ttctatcatc ggcgcacagg ttgcactgga tatggggcac ggcccaatta
     6841 ttgccgttgt cgcggcggta accaccggcg tgtttggcgg cgttttgcgc gatatgttct
     6901 gtaaacgcat cccactggta ttccagaaag agttgtatgc cggtgtctca tttgcctccg
     6961 ccgtgctgta catcgcgctg caacactatg tttctaacca tgatgtggtg attatctcca
     7021 ccctggtatt cggctttttt gcccgcttac tggcgctacg tcttaagctg ggattaccgg
     7081 tcttttacta cagccacgaa ggtcactaag gttcaaaacc tgtgatctgc tgggcagcca
     7141 gccaactgcc cagcttcttg atttgcgcat tttccttcca ttcaataacc tgtctggcgc
     7201 gcccagatcc agtccccggc agccgctgcc agaactgctc cgtgctaaat aaaagttgcg
     7261 accaggaccg ttcatcactg gcattaagcg ccgcccgggt taacggtatt cccattgcca
     7321 tcacccagcg agtaaaaggc tgcttacgag ccagattaaa ctgatgccat aactgcgcac
     7381 ttttactttt cgcgatcccc ggcgtgttct gtaattgctc tggcgttaat aaaagccagg
     7441 aaaagatatg ctcaaagcga tgagtctgat gcagcgcgcg ccagccggcc tcaccaatgc
     7501 catccagccc aagaacctgt tttgacccca gccagactaa gcgtgaaatg aactgttcct
     7561 gacaaacatc agaagcaaag tagcaggtca acgagttaaa gcggttttct ggtggtgtcg
     7621 gttttgtacg ttctgcaccg cgccagacca catcatcaat gcgaggaata ccctgaccgg
     7681 caaggctgac gaggatctga tcaccaggcg caatatccca ctcctgccag cgcctgacgg
     7741 aaccaatatt cacccgctgg acttttttat catccagcat gacaggtgcg agtgacgcaa
     7801 ccaccgatat tttaccgctc ttacccaccg caaactgaat cgccttcact tcggcaacct
     7861 gagctacagg ttgatatttc caggccacca accattctgc ctggcctggt agccaatggc
     7921 gggattcggg ttccttagcc gctcgcacaa ccacgccatc ggtgacgaag ggtaatttcg
     7981 ctttccacca ctcattgcgt acgcgcgcaa cttcatcagc atttttaacc gcacgggtat
     8041 acctctgcgt tagagtaaaa cctgcggtag ccagctcctt taaacgatcg gtcattaact
     8101 gcggtccatc cggccatgcc cagacaaaaa cagccagtga gtttagcgtg tcgctattcc
     8161 cctggcgcat catcaggcca gcaacttttg cgcgggcatt tattcccccc atttgttgtt
     8221 ggatatgccc ctcgcgctgg agaaatattt ccccctgaag cgtactgttg gctaaaggcc
     8281 cgctaacggt ttgcggcaca gcggaaatta agctaacttt ctgcgtccag tcctcgcctt
     8341 tcaggccatt accgcgactg attgctttgt tcagtttccc gtcccgataa accagggtta
     8401 ccgcaacgcc atcaactttt ggctgcaccc aaagatcgct acgttctcgc atccacagac
     8461 ttaatgcgtt tttatccacc attttgcgta cacccgtatg ggcaaccgga tgcataaccg
     8521 caccatttaa aggcggcatc atcacgtcgc ggggctcgct cccaaagcag cgttgccact
     8581 gcgtaagacg ggcgcttaac tgatcgtaaa caccgtcttc cacctcactt ttcccttcct
     8641 tccagtagtc atcgtcccac tgttttattt gctgttgcag gcgggaaatt tcttcctgtg
     8701 ctctggctgg cgaccaggcc ggacagaccg cccacacaga tgattgccag cacaagatac
     8761 ttattaatat cgccatccat actttcatca tcacctccgc tgtagatagt caggcagata
     8821 tacaacgtga tgaaagcaaa gccgagtggc aaaaacggag tctgcgagga cgcttcctga
     8881 aaatcgtctt tattgcaatg aatgacaggc aaatgcggaa gcagctacgc aaaacgcaac
     8941 aactttgcgc aaaaagtgtg agcaagggct acgtcacatg gccgcgccgt gtataataag
     9001 ctcgtatgta ggctttattt cgctaatcac atacgaaaga tactcatggc tcaaggcacg
     9061 ctttatattg tttctgcccc cagtggtgcg ggtaaatcca gcctgattca ggctttatta
     9121 aaaacccaac cgttgtatga cacccaggtt tctgtttcac acaccacacg ccaaccgcgt
     9181 cctggtgaag tccacggtga acattatttc tttgttaatc atgatgaatt taaagaaatg
     9241 attagcagag atgcgttcct cgaacacgca gaagtttttg gtaattacta tggcacttcg
     9301 cgtgaggcca ttgagcaagt actggcgacc ggtgtcgatg tttttctcga tatcgactgg
     9361 cagggcgcgc agcaaattcg ccagaagatg ccgcacgcgc ggagtatctt tattttaccg
     9421 ccgtccaaaa ttgaactgga ccgccgtcta cgcggtcgcg gtcaggacag cgaagaggtc
     9481 attgcaaagc gtatggcgca agctgttgca gaaatgagcc attacgccga atatgattat
     9541 ctgattgtga atgatgactt cgataccgcg ttgaccgatt tgaagaccat tattcgcgcc
     9601 gaacgtctgc gcatgagccg ccaaaagcag cgtcatgacg ctttaatcag caaattgttg
     9661 gcagactgaa cctgatttca gtatcatgcc cagtcatttc ttcacctgtg gagcttttta
     9721 agtatggcac gcgtaactgt tcaggacgct gtagagaaaa ttggtaaccg ttttgacctg
     9781 gtactggtcg ccgcgcgtcg cgctcgtcag atgcaggtag gcggaaagga tccgctggta
     9841 ccggaagaaa acgataaaac cactgtaatc gcgctgcgcg aaatcgaaga aggtctgatc
     9901 aacaaccaga tcctcgacgt tcgcgaacgc caggaacagc aagagcagga agccgctgaa
     9961 ttacaagccg ttaccgctat tgctgaaggt cgtcgttaat cacaaagcgg gtcgcccttg
    10021 tatctgtttg aaagcctgaa tcaactgatt caaacctacc tgccggaaga ccaaatcaag
    10081 cgtctgcggc aggcgtatct cgttgcacgt gatgctcacg aggggcaaac acgttcaagc
    10141 ggtgaaccct acatcacgca cccggtagcg gttgcctgca ttctggccga gatgaaactc
    10201 gactatgaaa cgctgatggc ggcgctgctg catgacgtga ttgaagatac acccgccacc
    10261 taccaggata tggaacagct ttttggtaaa agcgtcgccg agctggtaga gggggtatcg
    10321 aaacttgata aactcaagtt ccgcgataag aaagaggcgc aggccgaaaa ctttcgcaag
    10381 atgattatgg cgatggtgca ggatatccgc gtcatcctca tcaaacttgc cgaccgtacc
    10441 cacaacatgc gcacgctggg ctcacttcgc ccggacaaac gtcgccgcat cgcccgtgaa
    10501 actctcgaaa tttacagccc gctggcgcac cgtttaggta tccaccacat taaaaccgaa
    10561 ctcgaagagc tgggttttga ggcgctgtat cccaaccgtt atcgcgtaat caaagaagtg
    10621 gtgaaagccg cgcgcggcaa ccgtaaagag atgatccaga agattctttc tgaaatcgaa
    10681 gggcgtttgc aggaagcggg aataccgtgc cgcgtcagtg gtcgcgaaaa gcatctttat
    10741 tcgatttact gcaaaatggt gctcaaagag cagcgttttc actcaatcat ggacatctac
    10801 gctttccgcg tgatcgtcaa tgattccgac acctgttatc gcgtgctcgg gcaaatgcat
    10861 agcctgtaca agccgcgtcc gggtcgcgtg aaagactaca tcgccattcc aaaagcgaac
    10921 ggctatcagt ctttgcacac ctcgatgatc ggcccgcacg gtgtgccggt tgaggtccag
    10981 atccgtactg aagatatgga tcagatggcg gagatgggtg ttgccgcgca ctgggcttat
    11041 aaagagcacg gcgaaaccag caccaccgcg caaatccgcg cccagcgctg gatgcaaagc
    11101 ctgctggagc tgcaacagag cgccggtagt tcgtttgaat ttatcgagag cgttaaatcc
    11161 gatctcttcc cggatgagat ttacgttttc acaccggaag ggcgaattgt cgagctgcct
    11221 gccggtgcaa cgcccgtcga cttcgcttat gcagtgcata ccgatatcgg tcatgcctgc
    11281 gtgggcgcac gcgttgaccg ccagccttac ccgctgtcgc agccgcttac cagcggtcaa
    11341 accgttgaaa tcattaccgc accgggcgct cgcccgaatg ccgcgtggct gaactttgtc
    11401 gttagctcaa aagcgcgcgc caaaattcgt cagttgctga aaaacctcaa gcgtgatgat
    11461 tctgtaagcc tgggccgtcg tctgctcaac catgctttgg gtggtagccg taagctgaat
    11521 gaaatcccgc aggaaaatat tcagcgcgag ctggatcgca tgaagctggc aacgcttgac
    11581 gatctgctgg cagaaatcgg acttggtaac gcaatgagcg tggtggtcgc gaaaaatctg
    11641 caacatgggg acgcctccat tccaccggca acccaaagcc acggacatct gcccattaaa
    11701 ggtgccgatg gcgtgctgat cacctttgcg aaatgctgcc gccctattcc tggcgacccg
    11761 attatcgccc acgtcagccc cggtaaaggt ctggtgatcc accatgaatc ctgccgtaat
    11821 atccgtggct accagaaaga gccagagaag tttatggctg tggaatggga taaagagacg
    11881 gcgcaggagt tcatcaccga aatcaaggtg gagatgttca atcatcaggg cgcgctggca
    11941 aacctgacgg cggcaattaa caccacgact tcgaatattc aaagtttgaa tacggaagag
    12001 aaagatggtc gcgtctacag cgcctttatt cgtctgaccg cccgtgaccg tgtgcatctg
    12061 gcgaatatca tgcgcaaaat ccgcgtgatg ccagacgtga ttaaagtcac ccgaaaccga
    12121 aattaatgtt ttatgaaccc aacacgttat gcacgcatct gcgaaatgct cgccaggcgg
    12181 cagcctgatc tgaccgtctg catggagcag gtccacaaac ctcataacgt ttctgcgatt
    12241 attcgtaccg cagatgccgt tggcgtacat gaagttcacg ccgtctggcc tggtagccgc
    12301 atgcgcacca tggcttcggc agcggcgggt agtaacagct gggtacaggt gaaaacacac
    12361 cgcaccattg gcgatgccgt cgctcatctg aaaggccagg gcatgcagat tctggcaacc
    12421 catctttctg ataacgctgt cgatttccgc gaaattgatt acactcgccc gacctgcatt
    12481 ttgatgggac aggagaaaac gggcatcacg caggaagcat tggccctggc ggatcaggac
    12541 atcatcattc cgatgatcgg catggtgcag tcgctgaatg tttccgttgc ctcagccctc
    12601 attctttacg aagcccagcg tcagcggcaa aatgcaggca tgtacctgcg tgaaaacagc
    12661 atgttgccgg aagcagagca acaacgcctg ttgtttgaag gcggctatcc ggtgctggcg
    12721 aaagtcgcaa aacgcaaagg cctgccttat ccccacgtca atcagcaagg cgagatcgaa
    12781 gctgatgccg actggtgggc tactatgcag gctgcagggt aagtgccatg aaaggtcgcc
    12841 tgttagatgc tgtcccactc agttccctaa cgggcgttgg cgcagcactt agtaacaaac
    12901 tggcgaaaat caacctgcat accgtgcagg atctactctt acaccttccc ctgcgctacg
    12961 aagatcgcac ccatctctac cccatcggag aactactgcc gggcgtttat gccacggtgg
    13021 aaggcgaagt gctgaactgc aatatctcct tcggcggtcg gcggatgatg acctgccaga
    13081 tcagcgacgg ttccggcatc ctcaccatgc gctttttcaa tttcagcgcg gcaatgaaaa
    13141 atagcctggc gacgggccgc cgtgtactgg cttatggcga agcaaagcgc ggtaaatatg
    13201 gtgcggagat gatccacccg gaataccgcg tgcagggcga tctcagcacg ccagaattac
    13261 aggaaacgct cacgccggtt tatccaacaa cggaaggcgt aaagcaggcc acgctgcgta
    13321 aattaaccga ccaggcgctg gatctgctcg acacctgcgc cattgaagaa ctcctgccgc
    13381 cggaactgtc acaaggaatg atgacgctac cggaagcgtt gcgcactttg caccgcccgc
    13441 caccgacgct acagcttagc gatctggaaa ccgggcagca tccggcgcaa cgtcgtctga
    13501 ttctggaaga actgctggcg cacaacctca gcatgttagc cttacgtgcc ggagcacagc
    13561 gttttcatgc ccagccgctg agcgccaatg atgcgctgaa aaataaactc ctcgcagcct
    13621 taccgttcaa gccaacgggc gcacaggcac gcgtagtggc ggagatcgag cgcgatatgg
    13681 cgctggatgt gccgatgatg cgcctggtac agggcgatgt aggttccggt aaaacgctgg
    13741 tcgccgccct cgccgcgctg cgtgcgattg cccacggcaa acaggtggca ttgatggcac
    13801 caaccgaatt actcgccgaa cagcacgcca ataacttccg caactggttt gaaccgctcg
    13861 gtatcgaagt gggctggctc gccggtaagc agaaaggcaa agcgcgactg tctcagcagg
    13921 aagccatcgc cagcggtcag gtgcagatga ttgttggtac gcacgccatc ttccaggaac
    13981 aggtgcagtt taacggtctg gcgctggtga ttatcgacga acaacatcgc tttggcgtgc
    14041 atcagcgtct ggcgttatgg gagaaaggcc agcagcaagg cttccatccg catcagttga
    14101 tcatgaccgc cacgccgatc ccccgcacgc tggcaatgac ggcgtatgcc gatctcgata
    14161 cctcggtgat agatgaactg ccgccaggcc gcacgccagt gactacggtc gctattcctg
    14221 atactcgccg taccgacatc attgaccgcg tgcgccacgc ctgcatgact gaaggtcgtc
    14281 aggcgtactg ggtttgtacg ttgattgaag agtcagagtt actggaagcg caggcggcgg
    14341 aagctacctg ggaagagttg aaactggcgc taccagaatt gaatgttggc ctggtacacg
    14401 ggcggatgaa accagccgag aaacaggcgg tgatggcgtc gtttaaacaa ggtgagctac
    14461 atctgctggt tgccactacc gttattgaag tcggtgttga tgtgcctaac gccagcctga
    14521 tgattatcga aaaccctgag cgtctgggtc tggcgcagtt acaccagctg cgcgggcgcg
    14581 taggtcgtgg cgcggtggct tctcactgcg tgctactcta caaaacgccg ctttctaaaa
    14641 cggcgcaaat tcgcctgcaa gtgctgcgcg acagtaacga cggttttgtg attgcgcaaa
    14701 aagatctgga gattcgcggc cctggcgaat tgttaggcac gcgtcagacg ggtaatgctg
    14761 aatttaaagt ggcggattta ctgcgcgatc aggcgatgat cccggaagtt cagcgcctgg
    14821 cacgccatat tcacgaacgt tacccacaac aggcaaaagc cctgatagaa cgctggatgc
    14881 cggagacgga acgttactcg aatgcgtaaa aggcggcagt gagaagaccg ccatttcagg
    14941 ttaccctacc ttcctgccgg atgcgattca tcaccctaca aattcaataa attatgaatc
    15001 aatacgcagg cttgataagc gaagcgtatc aggcatttgt ctttgttgcg gcggcacttc
    15061 taccgccgct tcatcggtta accggcaaaa atcggcaaca tcaaatacaa cttaattacc
    15121 agcgcattga cgatatcgat aaagaacgca ccgaccatcg gcaccaccaa aaacgccatg
    15181 tgcgacgggc caaagcgttc agtaatcgcc tgcatgttgg cgattgccgt tggcgttgca
    15241 ccgaggccaa aaccacagtg acctgcagcc agcactgccg catcgtagtt tttgcccatc
    15301 atgcgccagg taacgaagat ggcatacaac gccatgaaga tggtctgtac caccagaatc
    15361 gccagcatcg gcagcgccag cgaagccagc tcccacagtt tcagccccat caacgccatc
    15421 gccaggaaca acgacaagct tacgttaccc agcacggata ccgcacgctc aaagacgcgg
    15481 taaaagccca ttattgacag accgttgctc agaatcacgc caacaaacag tacacagacg
    15541 aaggtcggca gttcaaaagc agtgccagcc aaaagttgcg caacaatttt ccccaccgtc
    15601 aggcagatag caatcagcgc gatagtttca atcagcacca acgaggtgat catgcgtccc
    15661 acatccggct tttcaaacgc cgtcgggact tcctggtcat ccggaatacc gttcggcgtg
    15721 gtggagtgtt tcaccagata gcgcgccacc ggaccgccaa tcaagccgcc cagcaccaga
    15781 ccgaacgttg cacaggccat cgccacttcc gtcgcattgg tgaagccata acgttcaatg
    15841 aacaatttac tccacgcagc gcccgtaccg tgaccgccgg aaagcgtaat agaaccggcc
    15901 aacagcccca tcagcggatc aagccctaac aggctagcca taccaatgcc aatggcattt
    15961 tgcatcacca acagaccaac aaccacaatc aagaagatgc caaccacacg cccaccggca
    16021 cgcaaactgg caatgttggc gttcaggcca atggtggcga agaaagccag cattaacgga
    16081 tcgcgcaggg acatatcaaa gttgacttcc cagcccatgc tttttttcag tactagtagc
    16141 gccagcgcca ccaacaaacc acccgcaaca ggttccggta tggtgtattt cttcaaaaag
    16201 gagacggaat ggaccaactt acgcccgagc agcaacgtca gcgttgcggc aacaagcgtt
    16261 gctaaagtat cgagatgaaa catagttact cctttgtatc cgcatcactc tttcatactc
    16321 gtcatacttc atcgcgcagt ggcaacttca tgaactatga cgggtatcga gttatgcttt
    16381 cctgggtcaa attctcagct gttaatgaac aaatggcatg gattttaagc agaaaaatac
    16441 caaaagttat ataaaaaagt gcatttatct catttaaata tttagatgag gatgtggcaa
    16501 acgtttgctt ttctctttcg gtcagctaaa atgcccgctt tgctaccacg ggattgtttt
    16561 cgatgtctgt ttccaccctc gagtcagaaa atgcgcaacc ggttgcgcag actcaaaaca
    16621 gcgaactgat ttaccgtctt gaagatcgtc cgccgcttcc tcaaaccctg tttgccgcct
    16681 gtcagcatct gctggcgatg ttcgttgcgg tgatcacgcc agcgctatta atctgccagg
    16741 cgctgggttt accggcacaa gacacgcaac acattattag tatgtcgctg tttgcctccg
    16801 gtgtggcatc gattattcaa attaaggcct ggggtccggt tggctccggg ctgttgtcta
    16861 ttcagggcac cagcttcaac tttgttgccc cgctgattat gggcggtacc gcgctgaaaa
    16921 ccggtggtgc tgatgttcct accatgatgg cggctttgtt cggcacgttg atgctggcaa
    16981 gttgcaccga gatggtgatc tcccgcgttc tgcatctggc gcgccgcatt attacgccgc
    17041 tggtttctgg cgttgtggtg atgattatcg gcctgtcgct aattcaggtg gggctgacct
    17101 ccattggcgg cggttacgca gccatgagcg ataacacctt cggcgcaccg aaaaatctgc
    17161 tgctggcagg cgtggtttta gccttaatta tcctgcttaa ccgtcaacgt aacccttact
    17221 tacgcgtggc ctcactggta attgcgatgg cggccggata tgcgctggcg tggtttatgg
    17281 gcatgttgcc agaaagcaac gaaccgatga cgcaagaact gattatggtg ccaacgccgc
    17341 tctattacgg tcttggcatt gaatggagtc tgctgctgcc gctgatgctg gtctttatga
    17401 tcacttcgct ggaaaccatt ggcgatatca cggcgacctc tgacgtttcc gaacagccag
    17461 tgtccggtcc gctgtacatg aaacgcctga aaggcggcgt gctggcaaac ggcctgaact
    17521 cgtttgtttc ggcggtgttt aacaccttcc cgaactcctg cttcgggcaa aacaacggag
    17581 tgatccagtt gaccggtgtt gccagccgct atgtcggttt tgtcgtcgcg ctgatgttga
    17641 tcgtgctggg tctgttcccg gcagtgagcg gttttgtaca acacattcca gaaccggttc
    17701 tgggcggcgc aacgcttgta atgtttggca ccatcgccgc ctccggtgtg cgtatcgttt
    17761 ctcgtgagcc gctgaaccgt cgggcgattc tgattatcgc gctgtcgctg gcggttggtc
    17821 tgggcgtgtc tcagcagccg ctgattttgc agtttgcccc tgaatggctg aaaaacctgc
    17881 tctcctccgg gatcgccgcg ggcggtatta ctgccatcgt gctgaatctg attttcccac
    17941 cagaaaaaca gtaattcact ggcggcggta agataattac cgccgcagtc tcacctcttt
    18001 aaccattccc gccttgagga ttgcgcgtaa atcgtgcata actcccttat gtgcatttca
    18061 cgggatggaa gaccatgaaa tttattggga agctgcttct ctacattctc atcgctctgt
    18121 tagtggcgat cgctggcctc tattttcttc tgcaaacccg ctggggagca gaacatatca
    18181 gcgcatgggt ttccgagaat agcgactatc atctggcctt cggggcgatg gttcaccgtt
    18241 tttccgcgcc atctcatatc gtgctggaga atgtcacgtt tggtcgtgat ggccagcccg
    18301 cgaccctggt ggccaaaagt gtcgacattg cgctaagtag tcggcaactg accgaaccac
    18361 gccatgtcga taccatcctg ctggaaaacg ggacgctgaa tctcaccgac cagaccgcgc
    18421 cgctaccgtt caaagccgat cgtctgcaac tgcgtgatat ggcgtttaat agcccgaata
    18481 gcgaatggaa actgagcgcg cagcgggtaa atggcggcgt ggttccgtgg tcaccagaag
    18541 ccggtaaagt gctgggtacg aaggcgcaga ttcagtttag tgccggatcg ctttcgctca
    18601 atgatgttcc tgccaccaat gtactgattg aaggcagtat tgataacgat cgcgttacgc
    18661 tgactaacct gggtgccgac atcgcgcgcg ggacattaac cggaaacgcg cagcgtaacg
    18721 ccgacggcag ctggcaagtg gaaaacctgc gcatggcgga tatccgtcta caaagcgaaa
    18781 aatcgctaac cgacttcttt gcgccattac gctctgtccc gtcgttgcag attggtcgcc
    18841 tggaagtgat cgatgctcgt ttgcaaggtc cggactgggc ggtgaccgac ctcgatctca
    18901 gcttgcgcaa catgaccttc agtaaagatg actggcagac acaagaaggc aaactgtcga
    18961 tgaacgctag cgagttcatt tatggttcgc tgcatttatt tgacccgatt ataaacgcgg
    19021 aattttctcc gcagggcgta gcgctgcgcc agttcaccag ccgctgggaa gggggtatgg
    19081 tcagaacgtc agggaactgg ctgcgtgacg ggaaaacgtt gatccttgat gatgcggcaa
    19141 ttgccggact ggaatatacc ttgccgaaaa actggcaaca gttgtggatg gaaacgacac
    19201 ccggttggtt aaacagcctg caactgaaga gatttagcgc cagccgcaat ctgatcattg
    19261 atatcgaccc tgacttcccg tggcagctca ccgcgctcga tggttacggt gccaacctga
    19321 cgctggttac cgatcataaa tggggcgtct ggagtggctc ggcgaatctg aatgccgccg
    19381 ccgcgacgtt taatcgtgtt gatgtccgtc gtccgtcgct ggcgctaacc gccaacagca
    19441 gcacggtgaa tatcagcgaa ctgagtgcat ttactgaaaa aggcattctg gaagccaccg
    19501 ccagtgtttc acaaacgcca caacgtcaga cacatatcag cctgaatgga cgcggtgtgc
    19561 cggtgaatat tttgcaacag tggggatggc ctgaattacc gttgactggc gacggcaata
    19621 ttcagcttac cgccagtggc gatattcagg ccaatgtccc gttgaaacct acggttagcg
    19681 ggcaattgca tgccgtgaac gccgcaaagc accaagtgac tcaaaccatg aatgcgggcg
    19741 tcgtttccag cggtgaagtt acatcggcag agctggtgca gtaaacatca atctcgccgg
    19801 gtacgactca gacgtacccg gcattcttca atagattaca acgtaattgt cagcgcattc
    19861 ccttgaggcg taaccaccag cccctgctca ctttcagcct gcgaaccgcc ttgcagacca
    19921 tttactttca caatattgcg caagcacagt gtccagttcc tcgcctcgcc cgcaccagtc
    19981 acagtaatcg tgttaccagt acgtgttgct tttagagtaa agatcaccga accgtcagca
    20041 gcgggcactt cacatacagc ttcatgcccg tcttgcagat taaagagatg gaatgccgtg
    20101 ccttcgtgcc acgcgtaatc gggacgttga tcgttgttgc ccagcgccaa tagggtgtta
    20161 tcacgcacat aaacgggcag actcaggaag ccgtgctgct gtttatgcca gcgactaccg
    20221 tcgagctcat tgttgtgcca caggtgtgtc cagcgacctt ccggcaagta gaactgcacc
    20281 tcgcccgcct cagtgaacac cggagcaacc atcacgttgt cgcctagcat gtattgacgg
    20341 tcaaggtaat cacaagccgg atcgtccggg aactccatca tcatggcccg catcatcggc
    20401 gtaccccgcg ctttcgcacg cgcagcttca cgatacagat acggcatcat gcggcatttc
    20461 agttgcgtga agaagcgcac cacatcacag gactcatcat cgtaggccca cggcacacga
    20521 taagatttgc tgccatgtaa acgactatgg ctggagagca aaccaaacgc gcaccagcgt
    20581 ttgtaaacgt gtgccggcgc ggtattttca aagccgccga tatcgtggct ccagaagcca
    20641 aaacctgaaa ggccaataga caaaccaccg cgcaggcttt ccgccattga ttcgtagtta
    20701 gcgtaacaat cgccacccca gtgtaccggg aatttctgcg caccgacgga ggccgagcgg
    20761 gcaaacaaga cagcttcttc ctcaccaacg gtgtccttga gcacgttcca caccagttcg
    20821 ttgtagatgt acgcataatg gttatgcatt ttctgcggat cggaaccgtc aaaccactga
    20881 acatcagttg ggatacgttc gccaaagtcg gtcttaaagc aatcaacgcc catcgcgacc
    20941 agacctttca gtttgtcggc gtaccatttg caggcatccg gattggtaaa gtcataaatc
    21001 gccagacc
//
LOCUS       DAZRUF010000051        20791 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZRUF010000051 DAZRUF010000000
VERSION     DAZRUF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20791
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.051"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHXOL_001525"
     CDS             complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHXOL_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR6808539.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1777..2090)
                     /locus_tag="ACHXOL_001526"
                     /pseudo
     CDS             complement(1777..2090)
                     /locus_tag="ACHXOL_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHXOL_001527"
     CDS             complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHXOL_001527"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR6808540.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLTCGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHXOL_001528"
     CDS             4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHXOL_001528"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR6808541.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHXOL_001529"
     CDS             6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHXOL_001529"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR6808542.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHXOL_001530"
     CDS             6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHXOL_001530"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR6808543.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHXOL_001531"
     CDS             7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHXOL_001531"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR6808544.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8330..10048
                     /locus_tag="ACHXOL_001532"
     CDS             8330..10048
                     /locus_tag="ACHXOL_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR6808545.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHXOL_001533"
     CDS             10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHXOL_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR6808546.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHXOL_001534"
     CDS             10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHXOL_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR6808547.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11359..12672
                     /gene="gltP"
                     /locus_tag="ACHXOL_001535"
     CDS             11359..12672
                     /gene="gltP"
                     /locus_tag="ACHXOL_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR6808548.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHRELLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHXOL_001536"
     CDS             complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHXOL_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR6808549.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHXOL_001537"
     CDS             complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHXOL_001537"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15262..15264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR6808550.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMDHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHXOL_001538"
                     /pseudo
     CDS             complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHXOL_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
     gene            complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHXOL_001539"
     CDS             complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHXOL_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR6808551.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="ACHXOL_001540"
     CDS             complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="ACHXOL_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR6808552.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20443..20718)
                     /locus_tag="ACHXOL_001541"
     CDS             complement(20443..20718)
                     /locus_tag="ACHXOL_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR6808553.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4679 a         5513 c         5695 g         4904 t
ORIGIN      
        1 tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat
       61 tttgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga
      121 aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa
      181 actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga
      241 agaaccagat gccgaggaat gccacggtga tagagaacag cgccgggtat tcatacggga
      301 agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta
      361 ctactgcggt aatcagcccc agccagccac ccatcatcgc gccacgcgtg gtcagtttcg
      421 accagtacat agaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca
      481 gccccaccat aaaggcgatg ttctgattct caaacagcac gccgaggata atcgcaatca
      541 cgccgaggat cagtacggtg atttttgata cccgcagctc ttcacgttcg gtcgcgcctt
      601 ttttgaagac gttagcgtac aagtcatgcg aaaccgcgga tgcgcccgcc agcgtcagac
      661 ccgcaaccac cgcgaggata gtggcgaaag caaccgctga aataaaaccg aggaacaggt
      721 tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc
      781 ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa
      841 taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg ctcttacgtg
      901 cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac
      961 caaacatcag tcccagaccc agagagagtg cggagatcgg atctttcacc agcccgcccg
     1021 gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac agattgttga
     1081 agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca
     1141 gcacggcttt gataatctgt acccaagtgg tcgccagcat gccgccgaac aggacgtaca
     1201 tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct
     1261 cgatcagttt acctgcgccc accatctggg cgataaggta aagcgccacc accaccagag
     1321 aaccacaggc cgaaagaata cgaatcggcc cttgtttcag acggtaagag gccacatcgg
     1381 caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc
     1441 agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg
     1501 cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct
     1561 ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgagttttg
     1621 atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct
     1681 gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga
     1741 aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag
     1801 gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag
     1861 aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt
     1921 cggattcagc ggcgtgccag ccagccgggc gcgaaggcga tcagtaaaat aaagccgata
     1981 taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt ttcgactaac
     2041 tccctgaaat gcgcattgtc ttctatccgc tgataaatag tgccattcat cacagattct
     2101 ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag gttaacgctc
     2161 gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg gcaatcaatg
     2221 cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc gttaccgact cgcatccggc
     2281 aattgtgggt tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgactac
     2341 gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt
     2401 gcgcagaata cggcgcataa ttttgccgga gcgggtttta ggcagggagt cggtccagtg
     2461 cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc acccagttgc ggacttctgc
     2521 gtacagttct ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt agatcgcctg
     2581 acctttaata ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg gatgcgccac
     2641 cagcgccgac tcaatctctg ccgtccccag acggtgaccg gagacgttca gcacgtcgtc
     2701 cacacgcccg gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa
     2761 atacatattt ttgaaggtgg agaagtaggt ctgttcaaaa cgttcgtgat cgccaaacag
     2821 cgtacgcgcc tgacccggcc aggagtcggt gatcaccagg ctgccttcgg tagcgccttc
     2881 cagcgggtta ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc
     2941 cgaaccggct ttcagctcgg tagcgccagg cagcggcgtg atcatgaaac cgccagtttc
     3001 ggtctgccac caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca
     3061 ctcccacgct tccgggttaa ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg
     3121 gtcggtaccc tcgatcgctt tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc
     3181 ggtatagaga atattgacct gatgcttgtc caccacctgc gccatacggg caggcgtcgg
     3241 ccagttgggt acgccttcaa acatcagcgt ggtcgcaccg caggtcagcg ggccgtacag
     3301 caagtaactg tgtccggtca cccagcccac atcggcggtg caccagtaga tatcacccgg
     3361 atgataatca aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt
     3421 atgcagcaca ccttttggct taccggtaga accggaggtg tagagaataa acagcggatc
     3481 ttcggcgttc atcttttccg cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca
     3541 ccacaggtcg cgcccttcct gccagtcaat tttcccgcca gtacgcttca gtaccaccac
     3601 atgctctacg ctggtgacgt tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg
     3661 aatactgcgc ccggcacgca caccttcgtc ggaagtgatc accagtcgtg agttggaatc
     3721 aataatgcgc ccggcaacgg cttccggcga gaagccgccg aaaatcaccg aatgcaccgc
     3781 gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat
     3841 cgccaccaca tcaccttttt taatgcccag ctcgagcagg gtattggcga agcggcagac
     3901 gtcgcggtgc agctctttat agctgatatg tttgctctgg ctggcgtcgt cgccttccca
     3961 gatgatggcg gtacgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag
     4021 attcagcgtg ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtgtt
     4081 tttcaccttc tggtaaggtt tgatccagtc aagaattttt ccctgttcgc cccagaaggt
     4141 atcaggtacg ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca
     4201 acggtctgcg atgttggcag gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc
     4261 cttgtagggt gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg
     4321 tttagtattt gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa
     4381 tttttgaagg tcagcaacaa aagttgatta attctttgag gaacatgcag ttatgcattg
     4441 ctgttgaaaa agaggaagat actaactaac tctaaagtgg tattttacat gcacttacaa
     4501 ttgattaaag acaacattca cagtgtggtt atttgttaca cataggggcg agcaatgtca
     4561 tgacagtgta ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gtaacaatgg
     4621 cgcaattcgg atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc
     4681 ttattgattc cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca
     4741 aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc cgcagcatcc cgatcaatat
     4801 ctctcctgga aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca
     4861 cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga
     4921 catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct
     4981 gaagatggtc cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg
     5041 atccagaaag acggcgaaga tggctacttc cacggtaagt gggcgcgcgg cggcccggaa
     5101 atcgtcaaca acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa
     5161 ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt
     5221 aaaccttttg agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat
     5281 gtggagtatt acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg
     5341 aaagtcgaaa atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc
     5401 ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc
     5461 attcacggta aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc
     5521 gaaggcaaac tctataccga ccataaaatt ggtaatccgt ttgataactt cgcccagact
     5581 tgtgcgaact gccataccca ggacaaagct gccttgcaaa aagtggtcgc ggaacgtaag
     5641 cagtcgatta acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa
     5701 gcgaaagcag cgctggatgc aggcgcgacg gaagctgaaa tgaagccaat tcaggacgat
     5761 atccgtcatg cccagtggcg ctgggatctg gcgatcgctt cccacggcat tcatatgcac
     5821 gcaccggaag aaggtttacg gatgctcggt acggcgatgg ataaagcggc ggatgcacgc
     5881 accaaactgg cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat
     5941 atctcaacca aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc
     6001 gagaagcagg acttcatcaa aacggtgatc ccgcagtggg aagaacaggc acgtaaaaac
     6061 ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga
     6121 atatgagcgt attacgttcg ttattaactg ccggggtgct ggcgtcgggc ctgttgtgga
     6181 gcctgaacgg aattaccgct acccctgcgg cgcaggcatc tgatgatcgc tacgaagtta
     6241 cccagcagcg taacccggat gccgcctgtc tggactgtca taaaccagat accgaaggta
     6301 tgcatggcaa acatgcttcc gttatcaacc cgaataacaa actgccggtc acctgcacca
     6361 actgtcacgg ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta
     6421 acgagccgat gtacaaggtt ggagagcaga acagcgtctg tatgtcctgt catctgccgg
     6481 aacagttgca aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca
     6541 gctgccattc cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga
     6601 ttaagatttg cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt
     6661 ctgttccgtt gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt
     6721 cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat
     6781 caatggggtg cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg
     6841 tatggatgct tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat
     6901 tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg
     6961 ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga
     7021 tgccgctagc gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat
     7081 cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg
     7141 cgatttctgc cgtaaaacca atttgcaggc cggtaagctg cccgcgtgtg ttgaggcttg
     7201 cccgacgaag gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact
     7261 gctgcgccag aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta
     7321 ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt
     7381 gaatcgctgg tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc
     7441 ggtctggtga cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac
     7501 agtacgttgc tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg
     7561 atcctcgtct tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt
     7621 tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg
     7681 ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg
     7741 aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa
     7801 acgctgatgc tggtgttggc ggtgctgctg ggggcttata ccggctttct gctgtcggcg
     7861 ctgaaatcgt atccgttcct caataacccg atcctgccgg tgctgttcct cttctccggc
     7921 atctcgtccg gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg
     7981 cattccacgg aagcgcactt tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc
     8041 ttcctgctgg tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg
     8101 ctggtggcgg cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg
     8161 ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct
     8221 gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt
     8281 ctctacgccg gacagttgac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt
     8341 ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc
     8401 gttgacggcc tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat
     8461 cggcattctg gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt
     8521 tctcatcagc gatttctcgg tcatttatgt cgcgcaacat agctatagcc tgctgtcgtg
     8581 ggaattcaag ctggcagcgg tgtggggcgg ccatgaaggt tcgctgctgc tttgggtgct
     8641 gctgctttcc gcctggagcg cgctgtttgc ctggcattat cggcagcaaa ccgatccgct
     8701 atttccgctg acgttagccg ttttatctct catgctcgcc gcactgctac tgtttgtggt
     8761 gctgtggtcc gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa
     8821 tccgatgctg caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg
     8881 cggtttgatg gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc
     8941 ggccagcgcc cgaatttgct ggcgctgggc gttacctggc tggagtgcat taacggcggg
     9001 gatcatcctc ggttcctggt gggcctactg cgaactcggc tggggcggct ggtggttctg
     9061 ggatccggtg gaaaatgcct ctttattacc ctggctttct gccactgcgc tgctgcacag
     9121 tttatccctg acacgccagc gggggatttt ttgccactgg tcgctgttgc tggcgatagt
     9181 tactctgatg ctgtcgctgc tgggcacctt aattgtccgt tctggcattc tggtttcggt
     9241 tcatgcgttc gcactggata acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat
     9301 tagccttgcg tctttggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg
     9361 tttttcgggg ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt
     9421 gctactgatc gtgctggtgg gaacgcttta tccgatgatt tacggtctgc tgggctgggg
     9481 acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat
     9541 gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa cgcgcgcagc ttccggcgct
     9601 ggtcgctcat gcgggcgtgc tgttatttgc cgcagggatc gtggtttcca gcgtcagccg
     9661 tcaggagatc agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt
     9721 ccgttttgag cgcctcgatc tgcaagccaa aggcaattac accagcgaaa aagcgatagt
     9781 ggcgctgttt gaccatcagc aacgcattgg tgaattaacg ccggagcggc gtttttatga
     9841 agcacgccgt cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta
     9901 tgcggtcatg ggtgaaaaaa ccggagcgga tcgttacgct tttcgtttgt atgtacaaag
     9961 cggtgtgcgc tggatctggg gtggaggatt gttgatgatt gcgggcgcat tgttaagcgg
    10021 atggcggggg aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct
    10081 gttttgcgcg cgctcaggtc gtagacacct ggcaattcgc caatccgcaa caacagcaac
    10141 aggcgttaaa tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactcg
    10201 aatccaatgc gccggtggcg gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg
    10261 ggaagaacga agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct
    10321 ataacccacc gttaacgggt cagacgctgg tgttgtgggc gctgccagtg gtgttgttat
    10381 tgctgatggc actgatcctc tggcgagtga gggctaagcg atgaaacagc ctcaaatacc
    10441 ggtgaaaatg ctgacaacac tcacgatatt gatggtattc ctctgtgtcg gcagttatct
    10501 gttaagccca aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca
    10561 tcagtttgcc agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg
    10621 cgctaatcca caaaacagcg aacaatgggc gttactgggc gagtactatc tgtggcaaaa
    10681 cgattacagc aattcgctgc tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc
    10741 tgaactgtat gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc
    10801 ccagacttgc gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct
    10861 gatgctgctg gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg
    10921 gcaaaaagtg atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat
    10981 taatatggca aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt
    11041 cattgagctg cataaaaaat aatcgaatga acatatgcca aaaataatca ctaatcagta
    11101 ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc
    11161 cataaactcg cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc
    11221 aacataccgc gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac
    11281 acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc
    11341 attgaggaag ttattctgat gaaaaatata aaattcagcc tggcctggca gattctgttt
    11401 gctatggtgc tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac
    11461 tggctggtcg tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg
    11521 attgttgtgc cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc
    11581 aaacagctcg ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc
    11641 gccatcattt tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg
    11701 tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc
    11761 agttcccacg gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg
    11821 atggcgaaag gcgaaatgct gccgatcatc tttttctcgg tgctgtttgg tctggggctt
    11881 tcttccctgc ccgcgacgca tcgtgaactg ctggtgaccg tgttccgctc catctctgaa
    11941 accatgttta aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg
    12001 attgcggtga cggtggctaa ctttggtttc tcttctctgt ggccactggc gaaactggtg
    12061 ctgctggtgc atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcgcgc
    12121 ctgtgcgggt taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg
    12181 tactccactg ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac
    12241 ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat
    12301 ggttcgacgc tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac
    12361 ctgtccatct ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt
    12421 gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa cgctgggtag cgtaggtatt
    12481 ccgctggaag gtctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact
    12541 gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa
    12601 tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact
    12661 gcggatcaat aattgaagat tgccggggat atccacccgg caatgtgtga atgcctgatg
    12721 cgacgcttgc cgcgtcttat caggcctacc ttaaccgttg gttaattttt caaactcttc
    12781 acacccggta tcaaaccctt ccatacagct caggttcaac cagtgcagcg ccttttgctt
    12841 gttcttctcg ataaaacctt cttcaccgtt taagaacatc attcccgccc agtactcgga
    12901 ataaccggtt cgggaaattg cagagctgcg tttgaaatac caggttgcct tgtcatcgtc
    12961 ggccttaatg cccacgccgt tggcgtaaat caaaccaagc agcatttggg catcgacggc
    13021 agagtcgttc tccagatctt ccgaggcgtt ttctaacagc gaaatagcct ttggataatc
    13081 cggtttaccc gcttgagtat ttaccagaat atgcgccagc gttacttcac cttctttact
    13141 gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt ggatagtcca gactgaccgg
    13201 attggtgatt ttgatctgcg ccagcagcgc gcaggcatca gcatcaccgc tgtcggcggc
    13261 tttctgtgcc caatattcgg ctttgctcaa atcgccggag ctaaaccagc tgtcggcaag
    13321 aaaatattgt gcgcgtcggt ccccggcctc tgctgccttt aaatactggc tgccaggctc
    13381 ggagtcgttg gcgtgggcaa agaatgtcaa aaacaacatc aatgcgataa tttttttcat
    13441 tttgataata cgattgggta gggagtaacc agtataaaga gttcgaatgg ataaaaaaac
    13501 agcctccgaa aggaggctgt agaaaggact gtattacgcc agtgccgctt cgcgcaggcg
    13561 agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct gatcggcgat
    13621 cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg ggcttaagtt
    13681 ttcggtaacc agctcgttac aggcaccaat ccaccactgg taggtcatgt aaatcgcccc
    13741 tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac gcgagtgcac
    13801 ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat tgatttgtgc
    13861 gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca tcgaacggca
    13921 agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtactcgt cggtgagttt
    13981 gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg gggtatcaaa
    14041 cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc ggcaaggcca
    14101 ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat ccgggcacag
    14161 atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat aacccatacg
    14221 ggtggcgatt tcactgatga tttgccagtc cgttttcaga tcccatttcg gttcaactgc
    14281 tttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat gctcgcccca
    14341 cgacgttgac ggcaaaatga catccgccgc cgacgcggtt ttggtcataa agatgtcctg
    14401 aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagct ccgcgtcagt
    14461 ttgcagcgga tcttcgccca taatgtacgc ggcacgcact tcgccatgcg ctgcgcggtg
    14521 cggcagctcg ctgatgcgat aaccggtatg agcaggcagg ctttccacgc cccaggcttt
    14581 ggcgaatttc tcgcggttag ccggatcttt cacgtactgg tatcccggat acgtatccgg
    14641 cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg ggtttacacc
    14701 cgcatgcggc ttaccgaggt taccagtcag catcgcgagg ctggtcagag aacgcacggt
    14761 ttccacgccc tggtagaact gggttacacc catgccccac aggatggcgg cgcttttcgc
    14821 ctgggcatac atccgtgccg cctgacgaat ctcactggcg ctgacgccgg tgatatcttc
    14881 aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc cttctgtacg
    14941 ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggt ccatcgcatt
    15001 caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa tgcgcgcggt
    15061 ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag cgttaattac
    15121 gtgattcgcc acgattgggt gggaatcagc cgggttgtac ccgaaaacga acactaaatc
    15181 ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg attggtgcag
    15241 acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat tggtaccaat
    15301 aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac cacgcgagga
    15361 gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca ggcgctcggc
    15421 aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac gctggcgacg
    15481 gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga aatcccagcc
    15541 ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg cctccgcccg
    15601 gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc aataggggca
    15661 aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc atacgcgctc
    15721 tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc tgatattacg
    15781 ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg acgtgacgaa
    15841 acgaaatacc gcgtgacagc catcacgcgg cagacatttt attttttctc gacgacggga
    15901 gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat cacccggcgg
    15961 ctgtccagca tcagtaatga catctcttcg gcaagtactg gcaaccgggc ttcagtggcc
    16021 tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg cagcgcgctg ggtgaagcgc
    16081 gttgcttcca cgcgttcagc ctgcatttct cgctcgtcgt tgagcgtttg cagacgcgtg
    16141 ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg ttcaatcatc
    16201 atgttgctgg cggcgcgcgt gccttcgaga ttggcattca accgtccacc gtcaaacagc
    16261 ggcaatttca gacccgggat gaagttgaac tggcgactgg tttttttgaa taaggtatcc
    16321 aggtggatgg catcaaggcc gaaaaacgct ttgatatcaa agctcgggta gaacagcgcc
    16381 cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc ttgcagatcc
    16441 gggcgtctgg cgagcaactc ataagagagt gtcgccggaa tgccggtctg gactcgtggt
    16501 aatgccaccg gtttgatctc cggcatatcg ctggcccccg cgccaatcaa tgcacgcaga
    16561 gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc gaccgccaga
    16621 atctgtgccc gcgcgccgtg gaaaggcact tgtgcttcca gaccgtgcgc cactttactc
    16681 tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag atcgagcatc
    16741 tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag cgatagctct
    16801 actgctgcgg tttctgccag cgcggcatta tgcgcgccaa tggcggcggc aaccgctgag
    16861 cgatgcacac cccacaaatc aagatccagt ccggcaaaca aacctactgt ggcttccgta
    16921 tagtacggcc cgtccatacc cagtgcgggc gcatccatcg cataagggct taaaaagccg
    16981 ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa ttgtgaaccg
    17041 gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc ttcggcgagg
    17101 gtgtgtgaac cacttagcgt ccgttggatc agcgcatcca gctacgggtc attgagttgt
    17161 ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcatc ggccagtttg
    17221 atttgttccg gtttgagctg ttgatgaggc gcagaatcct tgcgtaccaa ggcacagccg
    17281 gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag ttgacgattg
    17341 atcattgcgt ggctccttgt gcctgttccg tggcgggcgt taatgccggg gctgtccagt
    17401 ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag gtgggcagga
    17461 tggcctgcga ggcgggtgtc tcttcaagcg ttatttgagg agaacggctg agtgcggttg
    17521 ccagaccggc agcgtatttt tccagggcgt cggcaaactg acctgctctg tccggctgta
    17581 gtgcgttatc cagcgcctgc gccgaattcc aggtggcatc ccaggcgtga aggatatcgc
    17641 ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct tcgctgtcca
    17701 gttgacgctc cagcgccacg cgttggcaca tctcttcaca ggcattaaac gccgcatgca
    17761 gaccgatacg aatttgcaga taagtgcgca gagccgtgac ttcctgctgg cgtgggatcc
    17821 gcattacttt acttaacata cccagcgcgc cagccagttt ttgcggcagc gtgcgcgctt
    17881 cactttcagg ccagacaaag gtgtaaatca ccgcggacac cacggtacca atgatgatac
    17941 ccagggcgcg atcgcgaatt tccaccaggt cgtacactgg gccaaaaacg ttttcgagcg
    18001 tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt tcagagctgg
    18061 tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat tcgacaatat
    18121 tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg ccgcaaaagg
    18181 ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttgga ttagagacga
    18241 tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg taaaaggtgt
    18301 aacagatcaa acaggcgagc aatgttttta ccgcgtagcg tatatagtct ggattggtaa
    18361 aagcatcggc gaccattgat ggcggtttgg cggcgggcgt tggcggcgta ttcgggttca
    18421 tctggcccag ttgtaacaac gtctggcaga tattctccag gttacattcc cgtgccgcca
    18481 ccgcttcact ttcactgatc cgccagtcgc tttgccagca ctgaccttca gcaacggcat
    18541 gctgcagctt gttgatttct gaggctaatt tttgccgaaa ttcaataatt gcctgagaat
    18601 cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt accgttgcca
    18661 cgcagctttg ccaccatgcg ctttgagttc gccagttggc atcgtccgcg agacaaaaga
    18721 cattcagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt agcggtgcga
    18781 ggctgtctgt caggtgacta atggcatcat caagccgatc attaagcgcc tgatgcattt
    18841 gcgaaatggc acgactggga aaccacagca cgccgattaa cgtcatcagc aaggttggat
    18901 agaggccaac aacgatacac cacagcgtta agcgcacgac cacttccgga tagtcgagca
    18961 tggcggggaa ggtttgcccg taaatggcga caatggcgac agcgaaaaag accagtccca
    19021 gccgatgggt gcgcatcaaa aacatgcggc ccatcaggat cggcccggcg atgatcaatc
    19081 ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg atctccagca
    19141 ccgtggcaac cacaaacaag atcgccacaa atttggtgta aaacgcgttc gactgaatac
    19201 cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc atcgagatca
    19261 gaatcaccag caggcagcct acccagagtt gcacgatctg cggcacgcgg ccgggtcgcc
    19321 gctcgcttaa ctcttcatga aagaacgcca gcagcctgac catcggtaat ggcagggagt
    19381 tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga tgcggaacat
    19441 ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa cgcggaccca
    19501 gttaatagag cgagaaactt tcggcaggcc gcccagcacc aggccgccgt catccggtag
    19561 cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt cactcatcag
    19621 gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc ggaagttggc
    19681 gatcacgtac cagtgacgag tgtcgattag ggtaaaaata gggcgcatgg cagaagcaaa
    19741 ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc gaacggtcgc
    19801 catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac gttgcgccac
    19861 cagcgcatcg acgccgctga cggcgctggc ggctgactgc gcctgtaaca atacggcatt
    19921 aagatcggct tctgcggcgc gctgcgcagt tcttgcacgg tcaacgtctt ccgctgagac
    19981 aaaaccctct ttcagtaatg gctcggtacg gcgtaatgta tctgtggcct gtttcgcggc
    20041 ggcacgggct ttttctaccg tggcattaat cgagtcggca ccaaactgtt gcgcatcaac
    20101 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cagctttcgc
    20161 cagattggct tcgtacgggc gcgggtcaat gcggaacagc aaatcgccct gtttgactgc
    20221 ctggttgtcg gtgaccgcca gttctacaat gcggccgctg acttccggca ccacatcaat
    20281 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    20341 gaaaacaagg gcaaccagcg ccaatgcaac cactaacaga gcagggaatt tactgcgagg
    20401 agcttttttc ggcgtacttt ccatgagcgc gtctcaaata gattagaaaa atgccagcca
    20461 gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa atgccagatt
    20521 gatatttgcc cgctgaataa ctcgcctcgt gatgagcatc aaaataaggc tggcaatggc
    20581 gcagaaaaac cagctgggat aataagcacc gatcaccggg attgccggag ataatgagca
    20641 tcccgaaacc atgaagacgg gaattatcca ggctgttttt ttgagttgca tcgccatgcg
    20701 agagagaact gttggcatat taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa
    20761 tatcaatatc cattgaaggc gtcagtatgg c
//
LOCUS       DAZRUF010000052        20188 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZRUF010000052 DAZRUF010000000
VERSION     DAZRUF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.052"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..668
                     /locus_tag="ACHXOL_001542"
                     /pseudo
     CDS             1..668
                     /locus_tag="ACHXOL_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(733..1444)
                     /locus_tag="ACHXOL_001543"
                     /pseudo
     CDS             complement(733..1444)
                     /locus_tag="ACHXOL_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(1444..2685)
                     /gene="clsB"
                     /locus_tag="ACHXOL_001544"
     CDS             complement(1444..2685)
                     /gene="clsB"
                     /locus_tag="ACHXOL_001544"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR6808554.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2682..3443)
                     /locus_tag="ACHXOL_001545"
     CDS             complement(2682..3443)
                     /locus_tag="ACHXOL_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR6808555.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3576..3986
                     /locus_tag="ACHXOL_001546"
     CDS             3576..3986
                     /locus_tag="ACHXOL_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR6808556.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3948..5054)
                     /locus_tag="ACHXOL_001547"
     CDS             complement(3948..5054)
                     /locus_tag="ACHXOL_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR6808557.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5065..6207)
                     /locus_tag="ACHXOL_001548"
     CDS             complement(5065..6207)
                     /locus_tag="ACHXOL_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR6808558.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQIWQMQRAEDN
                     GQTFEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEA
                     LLSTEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLL
                     STLGMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPAR
                     YFVSTLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6200..7936)
                     /locus_tag="ACHXOL_001549"
     CDS             complement(6200..7936)
                     /locus_tag="ACHXOL_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR6808559.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7929..8924)
                     /gene="hlyD"
                     /locus_tag="ACHXOL_001550"
     CDS             complement(7929..8924)
                     /gene="hlyD"
                     /locus_tag="ACHXOL_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR6808560.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(8927..9598)
                     /gene="cecR"
                     /locus_tag="ACHXOL_001551"
     CDS             complement(8927..9598)
                     /gene="cecR"
                     /locus_tag="ACHXOL_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR6808561.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9827..11191
                     /gene="rhlE"
                     /locus_tag="ACHXOL_001552"
     CDS             9827..11191
                     /gene="rhlE"
                     /locus_tag="ACHXOL_001552"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR6808562.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11423..11905)
                     /gene="ybiA"
                     /locus_tag="ACHXOL_001553"
     CDS             complement(11423..11905)
                     /gene="ybiA"
                     /locus_tag="ACHXOL_001553"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR6808563.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12025..14175
                     /gene="dinG"
                     /locus_tag="ACHXOL_001554"
     CDS             12025..14175
                     /gene="dinG"
                     /locus_tag="ACHXOL_001554"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR6808564.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14203..15165
                     /gene="ybiB"
                     /locus_tag="ACHXOL_001555"
     CDS             14203..15165
                     /gene="ybiB"
                     /locus_tag="ACHXOL_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR6808565.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGIEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVAMGEAATISDGLARVNQAF"
     gene            15306..16391
                     /gene="hcxB"
                     /locus_tag="ACHXOL_001556"
     CDS             15306..16391
                     /gene="hcxB"
                     /locus_tag="ACHXOL_001556"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR6808566.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            16458..16517
                     /gene="ybiE"
                     /locus_tag="ACHXOL_001557"
     CDS             16458..16517
                     /gene="ybiE"
                     /locus_tag="ACHXOL_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR6808567.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(16620..16880)
                     /gene="ybiJ"
                     /locus_tag="ACHXOL_001558"
     CDS             complement(16620..16880)
                     /gene="ybiJ"
                     /locus_tag="ACHXOL_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR6808568.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17145..17411)
                     /gene="ybiI"
                     /locus_tag="ACHXOL_001559"
     CDS             complement(17145..17411)
                     /gene="ybiI"
                     /locus_tag="ACHXOL_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR6808569.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17485..18162)
                     /gene="ybiX"
                     /locus_tag="ACHXOL_001560"
     CDS             complement(17485..18162)
                     /gene="ybiX"
                     /locus_tag="ACHXOL_001560"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR6808570.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18204..20188)
                     /locus_tag="ACHXOL_001561"
                     /pseudo
     CDS             complement(18204..20188)
                     /locus_tag="ACHXOL_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4656 a         5299 c         5481 g         4752 t
ORIGIN      
        1 aggccaggat ataaaacggt atatggctca ttaccgcttt aggtttggtg gcaaccgcag
       61 gactagcctg gggagctaag tatctcgaaa ttacggcaac caaatatgat tcaccaccaa
      121 tgtatgtcgc tatagggtta ttattacttt gtatgtatgg cttaagtaag gatatcaaca
      181 agataaatgc cgccatcgcg ggcgtaatat atctgttttt actctctttg gtggcgattg
      241 tcgttgcaag tttagttcct gtatatgcca ttatcatcgt gttcagcact gcgggcgcga
      301 tgtttttaat cagtatgctg gccggtttat tatttaatgt tgagcctggt tctcaccgtt
      361 ttatcattat gatgacgttg acagggttgg ccctggtaat catcgtgaat gcggcattaa
      421 tgagtgaacg gcccatttgg gtaataagtt gcttaatgat tgtgttatgg ccaggcatta
      481 tctcgcatgg acgaaataag ctccttgaac tggcaggaaa atgccatagt gaagagttgt
      541 ggagtccggt tcgttgcgct tttacaggtg cattaacact ctattactat tttatcggct
      601 tctttgggat acttgccgcg atagctataa cgcttgtctg gcaaaggcat acgtgttttt
      661 ttcattagcc gtaaatgcac atactaaaga acttaactaa acttcacatc gccgcttcat
      721 ttttcgcccg tagcccagta caaagaaata gttcacgctt tgaccaagta acagccagat
      781 aatcgccccc attaccatcc agttaacgct ggaaatcagc atctgagcga gggcgaattt
      841 ccatgaaggc agcaccagct tttgcccttt gatggtcata tggcggtgct tcgcgaaagc
      901 gcaaaaccac aaataaaccg cgataatcat cagtaagcca atgccgagaa tacgcagcgt
      961 agtttgatcg acataccagt gatccggcaa ctccaccacg cctgcggtaa agataatccc
     1021 tgccagtaaa atatagccca gccagttggt ggtaatactg agcgagaaaa tccgcgtaat
     1081 agtgctaccg ggcaacccca gacgagagta caaacgatag cgcataccaa tgccgccgac
     1141 ccaggtactg agcgtcaggt tgaaggcgta gcagataaac gacaccagca tcacctggcg
     1201 cttcgccagt ttgtgaccgc agtaaaagcg ggcgagcagg tcatagcagc cgtaaatcag
     1261 atagctgacg accaccagcg accgcactaa gcagcgcaac gcgattgtag tcgcggatga
     1321 ccttccagac ctcttcccag tccacttttt tggcgtagac caccagcaac acaatcaccg
     1381 cgataaaaaa cagccaggtg aggatcttct ttgctaagcg ccagcgcggg tgtgatttac
     1441 tcatcagggt tttacccccg tgttttcagt ttctacccga tcctgcgttt ccattgtcgg
     1501 ttgtgcgggc ggatcaacct gcgccagacg tggcgtgtgt gccggaagcc agccaaccag
     1561 cgccgggaag tggcgtaaaa agtggaacgc cagcacgctt ttggtcaggt tccaccaggt
     1621 gcgtttgggc agcatggttt catccacctg ctgacaatct gcggcgataa tgccgttcag
     1681 attatcgcgc agcgtctggt taaaattacg atcgtggatg atgacatttg cttcgagatt
     1741 cagtgacaaa ctgagcggat cgagattact ggaccctact gtcgcccagt gatcgtccat
     1801 caatgctact ttgccgtgga gcgggcggcg gcggtactca aacacctgaa cgccgccttt
     1861 aaccagatag ttatacagca agcgcgcgcc gactctgaca atcggcatat ccggttcgcc
     1921 ctgaatgatc agtttgatcc gcaccccgcg gcgtgccgct ttacgcaagg cgtgtaaaaa
     1981 tcgatagccg gggaagaagt aggcgttggc gataatgact tcccgccgcg cctgagtgag
     2041 cattttcaga tagtggcgtt ctatatcatc gcggtgttct tcgttatcgc gccagaccag
     2101 caatacctgc gcgtctcccg gctggcggtt ctcttccgct ttgtgatgac gtcgccacca
     2161 gcgtcgtgcc gcgctctgtc caggcaggtt ttccagctca aactggagaa tatcttcgac
     2221 aatcggccct tcaaggcgta ccgcgtaatc ctgtttagcc tctggaccgt agctggacat
     2281 atgctcggcg gagtaattca gcccgccaat aaaggcgata cgcgcgtcga tcaccacaat
     2341 tttgcgatgc atccggcgaa acacattggt gcgcatacca aaaaggcgag ggcggggatc
     2401 gtagtagcgg aacactacgc cagctgccgt cagttcattg acaaactcat cgctgagatc
     2461 cggcgaaccg tagccatcca gcaagacttc cgctttaacc ccgcgttgcg ctgctgccag
     2521 tagtgccgca tgcagctgtt tgccgacgtt atcctcaaac cagataaacg tttcaagaat
     2581 gatgcgttct tgtgcctcgc caatcgcctt aaacaccgcg ggataatatt gctcgccgtt
     2641 ttccagcaac tggatcttat tgccttcgcg ccagctacat ttcataaatg aatctccgca
     2701 cttaaagggg catgatcaga aaggtgtcgc catgtccgca gcggcaacgc ggttggcgcg
     2761 ctggcgctgg catttttgac gtagatcctg tccagtcgta gtagaggaaa ttgcaccgga
     2821 aacgtgcgcg ccgggcgtcc gtgggcgcgg gtaaaaatct catccagtcc ggcctgcact
     2881 tttaacggat gattagcttt ttgccgccag tcattgaaat cacccgccac caataccggt
     2941 tcgccgtccg gtagctcatt cacccattcg gcgagcatcg caagctgcgc ctgacggtgc
     3001 gcctcacgca ggcccagatg tacgcacatc acatgaatcg cttttccggt catcggcggc
     3061 acaatgcggc agtagagcac gccgcgcttt tccgcaccat cgaccgaaac atcgcgattc
     3121 tcataatgtt caatgggata acgcgacagt acggcgttgc cgtgatgccc ttccgggtat
     3181 acggcattgc gaccgtaggc aaaatcgctc cacatagtgt cggcgagaaa ctcgtagtgc
     3241 gaggtatcgg gccagttttc cacatgcagc ggatgaactt cgtgcgcgcc catcacttcc
     3301 tgcaggcaaa caatatcggc gctgacggta cgcacggcat cgcgaagttc cggcaaaatg
     3361 aagcgtcggt taaacgcggt aaagcctttg tgaatgttga tggtgagcac cttgaacgaa
     3421 aattgttgtg tttgatcggg cataattttc ctgtctttgc ctcttatctc attgaaatag
     3481 tgtagtcggc gtcacaaaaa ggtgcggtct tacggaattt tccgtaaagt tcggtactct
     3541 gagtaagtag agataaattc ttcaggagag aagccatgaa gtggcaacaa cgtgttcgtg
     3601 tggcaacggg tctaagttgc tggcagatta tgttgcattt actggtagtg gcgctgctgg
     3661 tggtgggctg gatgagtaag actctggttc acgtcggcgt gggattatgc gcactgtatt
     3721 gtgtcacggt agtgatgatg ctggtgtttc agcgccaccc cgagcaacgc tggcgtgagg
     3781 tggcagacgt gctggaagag ctgaccacga cctggtattt tggcgcagcg ctgattgtgc
     3841 tgtggctgtt gtcccgcgta ctggaaaaca actttttgct ggcaattgca gggctggcga
     3901 tccttgctgg cccggcggta gtgtctttgc tggcgaaaga taagaagtta catcacctta
     3961 cgtctaaaca tcgcgtacgc cgctgaccct gtcgtggccg ttatcaccag tagcggccac
     4021 aaactattcc acacaatatc cagactcgca tccttcaaat aaatctgctt ggtaatgtcc
     4081 gtaaagtggc gaatagggtt aatccacgtc aggttttgca gccatactgg catgttttcc
     4141 accggagaaa cgtaaccgga aaggagaatg gcgggcatca taaaaacaaa cacgccgata
     4201 aacgcctgct gttgtgtcga gcagagtgat gaaatcaaca gaccgaatcc caccagcgat
     4261 aaaccgtaaa tcaccatcgt aaagtagaac agcgccagcg atccggcgaa ggggatttga
     4321 tacgcccaga taccaatcgc cagcacaatg gtggcctgga aggtggcgac aattaacgcc
     4381 ggtacggctt tgccgatgaa gatctgccag gtggtgagcg gcgaaaccag tagctgatcg
     4441 agcgtacctt gttcacgttc gcgggcgacg gaaagtgaag tgacgatcat tacgccgata
     4501 gtggtgatca tggcgatcag cgacggcacc acaaaccatt tgtagtcaag attcgggtta
     4561 taccagttgc gtaccaccag ctcgctgttg ttaggtttcg gttttccttc cagcagctcc
     4621 tgctgataat ttttgacgat ctgttgcagg tagttggcgg caatttgcgc actgttggag
     4681 ttacgcccgt cgaggatcaa ctgcaacggc gcggtctgga aggtatccag tttgcgcgag
     4741 aagtcagccg ggaaacgcac cagtagtaac gccttttgtg tgtcgatggt tgggcggatc
     4801 tcctgtgggc ttttcagcag cagcacatgg gtaaaggcgc tggcgcgggc aaaacgttgg
     4861 gtcagctcca ccgaatgctc gccgttatct tcatcgtaga tggcgatggt ggcgttagtc
     4921 acttccagcg tggcggcgaa cgggaacagg atcacctgaa taagcacagg taaaatcaga
     4981 atcgcgcggg tttgtggttc gcgcagcaac gactgcaact ctttgcggat taacgtccat
     5041 aagcgatgaa acatgctctt ctccctaatc cagccgacgt ttggttttca gccacgtcag
     5101 gccgataaac atcaccgccg aagcgatcaa aaacagcacg tttaccacca gcaccactgg
     5161 aatattcccg gcgaggaaca ggctttgcag ggtgctgacg aaataacgag cgggaataat
     5221 gtacgtcacc gcgcggatca ccgcgggcat actgtcgatc tgaaaaataa agccggaaag
     5281 cataatcgac ggcaggaacg cggcgttcag ggcgacctga gcggcattga actggttgcg
     5341 ggtaatcgtg gaaatcagca gccccatccc cagggtactg agtaaaaaca ggctggagat
     5401 aaaaaacaga ataagcagcg aaccgcgata cggcacgccg agaataaaca ctgacaccag
     5461 catacacagc agcatcgcca gcatcccgag aaagtaataa gggatcagct tacacagcag
     5521 cagttccgtg cgggtaatct ccgtagagag cagagcctcc atggtgccgc gttcccattc
     5581 tcgcgccacc accagcgagg tgagaatcgc gccgatgacc gtcatgatga tggtcactgc
     5641 accggggata atgaagtgct ggctaatcgc cgccgggtta aaccagtagc gggtttgtac
     5701 atcaataagc ggttcaaaag tctgcccgtt gtcctccgct cgctgcattt gccagatctg
     5761 ccagatctgc cagatccctt cgacataccc ctgtacaaag ttagcggtat tcggctcact
     5821 gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct gttccgcaaa
     5881 atccaccgga ataaccacca gaccgcgaat tttccccgcc tgcattttgg cgatcagttc
     5941 ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg tgtgggtgaa
     6001 atccagcgcc gcttcgctac gctgttccag caagatcccg acccgcagct tgctggagtc
     6061 gaggttaatg ccataaccaa aaataaacag cagtagcagt gggatcacta ccgcaatcag
     6121 ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg cccgcacgcg
     6181 acgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg tggatcaact
     6241 gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagccgac tgtgctttca
     6301 aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg atgcggtcgc
     6361 aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct ttctctacca
     6421 tgctgttgat atgtagccaa aattcacggc gggtgagggg atcaacgccg gacgtcggtt
     6481 cgtcgagaaa cagaatgtcc ggttcatgca tcagcgaaca ggccagcgcc agccgctgtt
     6541 taaaacctaa tggcagttca tcggtggcgt gggaggcgat acttttcagg ccgaacgcct
     6601 cgctcatgcg ggagattttt tcgttctgcg cccgaccgcg taagccatac acaccagaga
     6661 aaaagcgtaa attctgttcg accgtcaggt taccgtagag cgaaaacttt tgcgccatat
     6721 agccgagatg ctggcgcgct ttaccagaac tctctttcag atccatcccc agtaccagcg
     6781 cctgaccaga ggtcggcacc agcaaaccgc acatcatctt aaaggtggtc gatttgcccg
     6841 cgccgtttgg ccccagcaaa ccaaaaatct ccccgcgttt aacggcaaag ttgacgtgat
     6901 cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc accgtctcgc
     6961 cgggtgtgcc ttctaccgta tgtaatattg cgcccagcgg cgattccgag gttccggcac
     7021 cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg atgttgattt
     7081 ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggt ctcttttttg aggatcagac
     7141 gtaccgattt cccctgaatc atgccgtcgc tgacctgcgg cagtttcaat gcccgttgca
     7201 acagtttgcg gttgccctcg tgtggacttg tcatcagaaa gctgcgtccg gccatggttt
     7261 gtgtcagggc ttttggttcc ccctgataca gcaactcgcc ttcattcatc agtaacacgt
     7321 cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac atcccttcgc
     7381 ctgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca acgccgacgc
     7441 cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc aggccgagtt
     7501 tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc ccaagagacg
     7561 taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg ctgcgcaaat
     7621 ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca aatttctgcg
     7681 gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gattggatca aagccaatca
     7741 ccgtggcatt gccgctgtcg ggtttcagta atcccgccag catccgcatt agcgtggttt
     7801 tacctgcacc atccggcccc accagccccg tcacataacc ggcgtgaatg gtacaatcga
     7861 gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg ttcagcgtga
     7921 taacggcatc attcatgtcc tgcctcgtta ccgaattgca ccgtcaccgg cattccctgg
     7981 cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag gtcggtacgc
     8041 agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac gaaaccaatc
     8101 tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt gcgccccggc
     8161 tgggcctggt caagataccg ttcatcaaca taagcgcgca cccacaccgg acgcgttagt
     8221 gaaacggtaa acaccgtgcc accttcattg aggaccgtgc ctggctccac cgcgcgcgtt
     8281 aacagcgtgc catcagacgg ggctatcaac gttgagtcct gtaaattcaa ctccgcctgc
     8341 gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gggcgatgtc ctgttcacgg
     8401 ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc ctgcgcctgg
     8461 tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tagtgcggct tttccacagt
     8521 ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc ctgtttcacc
     8581 gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag atcatactgc
     8641 gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata cggcttgtga
     8701 tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac cgccagcgat
     8761 tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac gttgccatac
     8821 agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc aaccacggcg
     8881 gcaagtacca ctaccgccag ttcgatcacg acaggttttt tcatcactac aaactccttt
     8941 gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg atcagttcgg
     9001 tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct ttgccaagac
     9061 ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg gtgtcattgg
     9121 cgtcgctgcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc ggactaatca
     9181 cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg gagataaact
     9241 tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg caggcgcgaa
     9301 ggagcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag cgttcggctt
     9361 cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg gcgcaggcga
     9421 ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc tgcccggctt
     9481 gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc gccagtgcgg
     9541 cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga ttattcatcg
     9601 cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg gagttgtcca
     9661 gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct gcgtcacaaa
     9721 aactgctaca ctccgctcgc tcatgacatt gtggtttttg tcattttcct tttcagtatc
     9781 tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagtcatgt ctttcgatta
     9841 tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc gtgaacccac
     9901 ccctattcag cagcaggcga tccctgcggt gctggaaggc cgcgacctga tggctagcgc
     9961 ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac acctgatcac
    10021 tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta ccccgacccg
    10081 tgaactggcg gcgcagattg gcgaaaacgt ccgtgattac agcaaatacc tgaacattcg
    10141 ttcgctggtg gtgtttggtg gtgtgagtat taacccgcag atgatgaaac tgcgtggcgg
    10201 cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc agaatgcagt
    10261 gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc tcgacatggg
    10321 ctttatccac gatatccgtc gcgtgttaac aaaactacct gcgaagcgcc agaacctgtt
    10381 attctccgcg accttctctg acgatattaa agccctggcg gaaaaactgt tgcacaaccc
    10441 gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc agcacgttca
    10501 ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggga aagggaactg
    10561 gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg ctgaacagct
    10621 caataaagat ggcatccgta gtgcggcgat ccacggcaat aaatcgcaag gtgcgcgtac
    10681 tcgtgcgctg gctgatttta aatcgggcga tattcgtgta ctggtggcaa ctgacatcgc
    10741 tgcgcgcggc ctggatattg aagagctgcc gcacgtcgtc aactatgaac tgccaaacgt
    10801 accggaagat tatgtccatc gtatcgggcg taccggtcgt gcggctgcta ccggtgaagc
    10861 gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa aactgctgaa
    10921 aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa tcaaagccga
    10981 accgatccag aacggtcgcc agcaacgtgg cggcggcggt cgtgggcaag gtggtggtcg
    11041 cggtcaacag caaccacgcc gtggggaagg tggcgcaaaa tctgcaagcg cgaaacctgc
    11101 agaaaaaccg tctcgccgcc tcggcgatgc caaaccggca ggcgaacaac aacgtcgccg
    11161 ccgtccgcgt aaacctgccg ctgcgcagta atcttttatg ccgggctatg cccggcatca
    11221 ggctgatgaa caaacgcaaa actgcctgat gcgctacgct tatcaggcct acgtgaactc
    11281 tgcaatatat tgaatttgca tgcttttgta ggccggataa ggcgtttacg ccgcatccgg
    11341 catttcacaa caagcacttg tcagcaattt gagaacacgg gaaaaaattt atctgtttta
    11401 ccgcccataa gccaccgaga cgttacttct ctatagccaa ttgttctcgc aactccatta
    11461 aaaggtagcc cagtcgattc ttgcccttac cattaccacc gtctccccag taagcatcgt
    11521 tttccgtatg ctcaaccagt tttgcgggcg cggttgccag caagagcaca cgcagttctg
    11581 catgctgttc gaatttagca cgaagtgctt tacgcatcac ctgttcttta accgactccc
    11641 agtttttacg aagtggcttg gaacgattac gccccatgcg agcggcgacc atgggggaag
    11701 aaacccgacg gatctcttcg cggtattttt catcaaggaa tttttgtgcc tgaaaatagt
    11761 gttcggaggt gggccaggtt ttcccgtcaa ccttgatggg ccaggcggca aagttagaaa
    11821 aatcaccgta gtcatcgctg gtgctgtaga aatttatgat ggtgtcttgc atgacgtgtt
    11881 ggatcctttg tgctcgaacg ggcattaaac cgcattatgt tggtggttat tgcgagccgc
    11941 tttccagaaa cagaaaaacc attacccctg aaaaccgaaa aatgccacaa tattggctgt
    12001 ttatacagta tttcaggttt tctcatggca ttaaccgccg cgcttaaagc gcaaattgcc
    12061 gcctggtata aggcgcttca ggaacagatc cccgacttta ttccccgtgt gccgcagcgg
    12121 cagatgattg cggacgtcgc caaaacgctg gccggagaag aagggcggca tctggcgatt
    12181 gaagccccca ccggcgttgg gaaaacgctc tcctatttga tccccggcat cgccattgcc
    12241 cgcgaagagc aaaaaacgct ggtggtgagt accgccaacg tggcgttgca ggatcagatt
    12301 tacagcaaag atttaccgct gctgaaaaag atcattcccg atcttaaatt cactgccgct
    12361 ttcgggcgtg ggcgctacgt ttgtccgcgt aatctgacgg cgctcgccag cactgaaccc
    12421 acgcaacagg atctgctggc gtttcttgac gacgaactga cggcaaataa tcaggaagag
    12481 caaaaacgtt gtgcgaagct gaagggcgat ctcgacactt atagatggga tggtctgcgc
    12541 gatcatacgg atatcgctat agatgatgat ctctggcgtc gtttgagtac cgacaaagcc
    12601 agctgcctca accgtaactg ttactactat cgtgaatgcc cgttttttgt tgctcgtcgg
    12661 gagattcagg aagcggaagt ggtggtggca aaccatgcgc tggtgatggc ggcgatggaa
    12721 agcgaagccg tactgcctga cccgaaaaat ctgctgctgg tgctggacga aggtcatcac
    12781 ctgccagatg tggcccggga tgcgctggag atgagcgctg aaatcaccgc gccctggtat
    12841 cgactacagc tggacttgtt cacgaaactg gtcgctacct gcatggagca gtttcgcccg
    12901 aagaccatcc caccgctggc gatccctgaa cgtttgaatg cgcattgtga agagttgtat
    12961 gagcttatcg cctcgttaaa caacattctc aatctctaca tgcctgccgg gcaggaggca
    13021 gagcaccgtt ttgcgatggg cgaactgcct gatgaagtgc tggagatctg ccagcggctg
    13081 gcaaaactca ccgagatgct gcgtggtctg gcggagttat ttcttaacga tttaagtgag
    13141 aaaaccggca gccatgacat tgtacgcctg catcggttga ttttgcagat gaaccgcgcg
    13201 ttggggatgt tcgaggcgca aagcaaactc tggcggctgg cgtctctggc gcaatcttcc
    13261 ggtgcgccgg tgaccaaatg ggcgacgcgg gaagagcgcg aagggcaact gcatctctgg
    13321 tttcactgcg tggggatacg tgtcagcgat cagctggaaa ggctgctgtg gcgcagtatt
    13381 ccgcacatta ttgtcacctc cgcaaccttg cgttcgctga atagtttttc gcgtttgcag
    13441 gagatgagtg gtctgaaaga gaaagcgggc gaccgttttg tggcgctgga ttcccccttt
    13501 aaccactgcg aacagggcaa aattgttatt ccccggatgc gcgttgagcc ttccatcgac
    13561 aacgaagagc agcatattgc cgaaatggcg gcctttttcc gtaagcaggt ggagagcaaa
    13621 aaacatctcg gtatgttggt actgtttgcc agcggacggg cgatgcagcg ctttctcgac
    13681 tatgtgacgg atttacgtct gatgttgctg gttcagggcg atcagccgcg ttaccgttta
    13741 gttgaactgc accgcaaacg cgtcgccaac ggtgagcgca gcgtgctggt gggcttacag
    13801 tcatttgccg aagggcttga tttgaaaggt gatctgctca gccaggtgca tatccacaaa
    13861 atcgcttttc cgcccatcga cagccccgtg gtgatcaccg aaggggaatg gctgaaaagc
    13921 ctcaaccgct atccgtttga ggtgcaaagc ctgccgagcg cctcgtttaa cctgattcag
    13981 caggttgggc gactgattcg aagccacggt tgctggggcg aagtggttat ctacgacaaa
    14041 cgcttgctga ccaaaaatta tggcaagcga ctactggatg cattaccggt atttccgata
    14101 gagcaaccgg aagtccctga aggtatagtt aaaaagaaag aaaaaacgaa atccccacgc
    14161 cgtcggcggc gttaatgatg tgagtcaggt aaggagtcgt aaatggacta tcgcaaaatc
    14221 attaaagaga tcgggcgcgg gaaaaaccac gcgcgcgatt tagaccggga tactgcgcgc
    14281 ggtctgtatg ctcatatgct caatggtgaa gtccctgacc ttgagttggg cggcgtattg
    14341 attgcgctgc gtatcaaagg agaaggggaa gcagagatgc tcggctttta cgaagccatg
    14401 caaaatcaca ccatcaagct gacgccgcca gcaggcaagc cgatgccgat tgtcatcccc
    14461 agttacaacg gcgcgcgtaa acaggccaac ctgacgccgt tgttggcgat tctcctgcac
    14521 aaactcggtt ttcctgtggt ggttcacggg gttagcgaag atccaacccg cgtgctgact
    14581 gaaaccattt ttgaattgat gggtattacg ccaacgttgc acggcgggca ggcgcaggcg
    14641 aagctcgacg aacatcaacc ggtgtttatg ccagtcggcg cgttttgccc gccgctggaa
    14701 aaacaactgg cgatgcgctg gcggatgggc gtgcgtaaca gtgcacatac cctggcgaaa
    14761 ctggcgacgc catttgccga aggtgcggcg ctgcgtcttt ccagcgtttc gcatccggaa
    14821 tatattggac gcgtcgcgaa gttctttagc gatatcggcg ggcgggcgct actaatgcat
    14881 ggtattgaag gtgaagtgta tgctaatccg cagcgctgcc cgcagatcaa tctcattgac
    14941 cgtgaaggga tgcgggtgct gtatgaaaaa caggacactg ctggtagcga gttactgcca
    15001 caagcaaaag atccggaaac cacggcgcag tggattgagc gttgccttgc tggcagcgaa
    15061 ccgattcccg aatcgctgaa aatccagatg gtttgctgcc tggtggctat gggtgaagcg
    15121 gcaactatca gcgacggcct ggcgcgcgtt aatcaggcat tttaattctt ttctcagccg
    15181 gatgagccat gtcatccggc ttttacccca ccgcaatatg aaattcctgc atctttattg
    15241 accttcccac gcccggcgtg cagcataaaa atacaacaaa cacataacat aaacaggagt
    15301 taaccatgga aagtggtcat cgctttgatg ctcagacgct gcacagtttt attcaggctg
    15361 tatttcgtca gatgggtagc gaggaacaag aagcgaaatt agttgccgat catttaatcg
    15421 cggcaaacct ggcagggcat gattcacatg gtattggcat gatcccaagc tatgtacgct
    15481 cctggagtca ggggcacctg caaattaacc atcatgccaa aaccgttaaa gaggcggggg
    15541 cggcggtcac gctcgatggc gatcgcgcat ttggtcaggt cgcggcacat gaagcgatgg
    15601 cgctggggat tgagaaagcg catcagcacg gtattgccgc cgtggcgcta cataactcgc
    15661 atcatatcgg ccgtatcggt tactgggcgg agcagtgtgc agcggcgggg tttgtctcta
    15721 tccactttgt tagcgtggtc ggtattccaa tggtcgcgcc gttccacggt cgcgacagcc
    15781 gctttggcac caatccgttc tgtgtggttt tccctcgtaa agataatttc ccgctgttgc
    15841 ttgattacgc caccagcgcc attgcatttg gcaaaacccg cgtcgcctgg cataaaggcg
    15901 tccccgtgcc gccaggttgc ctgattgacg ttaacggcgt gccgacgacc aatccggcgg
    15961 taatgcagga gtcgccgttg ggttcgctgt tgacctttgc cgaacataaa ggctacgccc
    16021 ttgcagcgat gtgtgaaatt cttggcgggg cgctttccgg cggtaaaacg acgcatcagg
    16081 aaacgttaca aaccagtccc gatgccattc ttaactgcat gaccactatc atcatcaacc
    16141 cggaactctt cggcgcgccg gattgtaacg cgcagaccga agcctttgcc gagtgggtga
    16201 aagcctcgcc acatgatgat gataagccga ttttgctacc gggcgagtgg gaagtgaaca
    16261 cgcgtcgcga acggcagaag caggggattc cactggatgc gggaagctgg caggccattt
    16321 gtgatgcagc gcggcagatt ggtatgccgg aagagacgtt gcaggctttc tgtcagcagt
    16381 tagccagcta aaaaaaagcc cgtccagtgg cggacgggca aacaagggta acataggatc
    16441 aatgagggtt agagcatatg cgtctgtcgg caaacagaca gggaaatact tgtgctggac
    16501 gtagcgtaaa cgcctgatcc gtccaacggt tcggattttg taggcctgat aagacgcggt
    16561 agcgtcgcat caggcatctg ttgccggatg cggcgtaaac gccttatccg tcctacgaat
    16621 tacttataaa ttaccgcagt accgcttaat ttattgttgt tggtggcgga agtgatgctg
    16681 tatccactgg caccggctgc cgcggctttc tcagccagtt tcgcttccag ggcatcgagg
    16741 gtggatgcgc catcggcaga aaccacgccg attttattca tgttctgtgc ctgggatgcc
    16801 gttatcggtt ccgccgcgaa cacgccaaat gacagagttg aaagagccat agcagcaaca
    16861 acagtattga tagttttcat aattaatctc tcgcaggtga tttttgtata agtgacgttg
    16921 tttcgtcgat gtgatgagta tcacgttttt ttacgagaga taaaatcgaa gagaattgac
    16981 cgccttgttc aaataaattg attgatatct aacatactga taaaccattt atttgttgta
    17041 cttatgaact tgttttatac cgcgtggcag tcacagcagc gttaaaggca cggaatgcca
    17101 cgcaaaataa aaattatact cgctatgcat ctcgccagtt gtagttaacg taactggctg
    17161 tctttcgaac ctctgcgatt atatcctgta taagctggtt tttgtaaatc tttctcctgc
    17221 tgacaatgaa tacataagcg cacgccagga atggcttccc gacgggcctg cgggatgggg
    17281 gcaccgcact cttcacattc atccaggctt tcgccgcgcg gaatttcacc ccgagcgcgg
    17341 gcaatcgcat cttcaattgt actgttgatc tgttcgttga cggcgtcatc gttagcccaa
    17401 ccggatgcca tatcgacctc cccatatcaa tacttgtaca gttaagtgta gctaatccag
    17461 ggacgaactc gggcagttca agcatcagat ctccgaccat tcccgcagca gattatgata
    17521 aagattaagc agcgacagga tctcttcact ttcgccgtag cggcttttca gcgactgaat
    17581 attgttgtcc agttcaaaca gcatggcgcg ctttttatca tcgcggatca tcgactggat
    17641 ccacataaat gatgccactc gtacgccgcg ggttacgggt ggcacgcaat gctggctgct
    17701 ggagggatac aacacgagat cgcctgccgg gagttttacc cgatgttgtc cgaaggtgtc
    17761 attaacgacc agttcgccgc cgtcgtagct ttgtggatcg cttaaaaaca gcgtggcaga
    17821 aaggtcagta cgcatccagc cgttttgtgg atggctgcgt actgcgccat ccacatggaa
    17881 accataggtt tcattgttct gatagcgatt aaacagcggc gtggaaaggg tacgcggcaa
    17941 ggccgcggca aagaacaaag catgttggtt aaccgcgttc agcacctcat tttgcagggc
    18001 ggcgtataac gtgctgcggg tgtcgacctg ttgattgttc ttaacttgcg caccttgtgc
    18061 gccggtggtg acgcgtccat ccacccattc ggcttgttcc agttgttcgc gaaaccgggc
    18121 gacgtcctgt ggcgataaca cgccgggaat gtggtacatc atacaagttc tccaaaaagt
    18181 ggggcctgcg ccccacatct gaatcagaaa tgcatattgg ctgtgagcaa gaaggttctt
    18241 ggctcgcccg ggtgataacg gtagccgctc ttgttgattg aggcgacgta atcagtatca
    18301 aacaggttgt agacgtttag ctggaagtcg agattgcgat taacccgata ccccagtttg
    18361 gcatcggcga cccagtaacc ttcggtaaat gctggcgttc ccaccgcgcc gtctgaacct
    18421 ttatgcatac tgccgatata gcgtgcgccc gcgccaacag agatatcgtc ggttgcctga
    18481 tattggctcc ataaggtgaa ggcgtgctcc ggggtatacg gcagcgatga ggaaccatcc
    18541 tgggcaacat ctttgccgtt tttgatggtt gctttttgct gggtatagcc gccaatcacc
    18601 tgccacgcgg gagtgatatt cccggccacg gatatctcat agccttcgac gcgtttctta
    18661 ccgtattgcg agtaagttcc gtcatcattt tgctcaactt cattttcgat atcagtacgg
    18721 aacagcgcgg cggtgagcaa cagacgttta tccagaacct gccatttggt gccaatctcg
    18781 ctggtgttgg ctttttgcgg tttaaaatcg gtgcggttgg cactgttacc gctgccagac
    18841 tgcgcaaggg cgaagttgtt gccgcccgga ggctgctggg aaacggcata gttaatatag
    18901 acattgccgt tttccgtcag gtgatacagc gccccggctt tccagttcat cagattgccc
    18961 gacttggcgg tgtcgacggt ggtgaccgga gaaccttttg ccacaccagt tgggcaggtg
    19021 atggcaccgc gtccgctgcc gccgcaggcg gtggcactgt catattcagt atgataatta
    19081 tccagacgga tcccgccgtt cagctcaaaa tcacgggtga tttgcagcgt atcaaaggcg
    19141 taaattgcga aggtatccgt ctgaccattg gcgtttgcgc cgttgcgcgt caggccgccg
    19201 ggatgaatgc tgctgtcagg atgataaata tttaccgcgg gtaacgtcac cggattaacg
    19261 ccgtagttag tctgcgtttc acgggtaaat tccacgccgg tactgacatc atgaccgata
    19321 gaaccggtat agaacgtcga ggtcaggttg gtctggttgg tgagaatttt attactcaca
    19381 tctttggtat tcgccgtgcg tgaccaggtt cagctattca catcgctggt gggctgagta
    19441 atattcgacg ccccgcccat aatcgccgtc atcaggtaat cctgctttac gcgcgaccaa
    19501 cgggtagtat tgcgaatggt ggtgttatcg ttgatgtcgt gctcaaaacg catggtggcg
    19561 gtgtcggtgg tcgaatcgtc gtaatcggaa tccgtgccgt aaaagttatg agtatcaact
    19621 tttccggaat gattcagggc cgccgttccc gcagatgggg cagaatagcc cggcaaaccg
    19681 atggtcggaa tgccgccgtc tggcgtgttg tgctgggtga catgcagata attaagatac
    19741 aaacgattcg ctgtaccaag gccaaaagcg acagaagggg cgacgccgta acgctcattt
    19801 ttgactttgt cgcgtccggc atcgtgcgtt ttttcgccca ttacattcag gcgcaccgcg
    19861 gtggtatcac caatgacctg attgacgtcc agcgtgccgc ggcggaacca ggcgctgcca
    19921 atactggcgg aggcgtcaat gccggaatca ttgcgcggct gtttgctgat catattgatc
    19981 gagcccgtcg gtgcgctgcg cccgtagtcg gtgccggacg gccctttaat cacttcgacc
    20041 tgctcggtat tgaaggtgtc gcgcgagacg ctgccgatat cgcgaatgcc atcaatataa
    20101 atactgttag aggtatcggc accacgcata taaatggcgt cgccagtggt ggagttaccg
    20161 ttctcacccg caaaaaacgc acccacgc
//
LOCUS       DAZRUF010000053        20146 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZRUF010000053 DAZRUF010000000
VERSION     DAZRUF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20146
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.053"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(210..1022)
                     /locus_tag="ACHXOL_001562"
     CDS             complement(210..1022)
                     /locus_tag="ACHXOL_001562"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR6808571.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            1284..2045
                     /gene="udp"
                     /locus_tag="ACHXOL_001563"
     CDS             1284..2045
                     /gene="udp"
                     /locus_tag="ACHXOL_001563"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR6808572.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2186..3613
                     /gene="rmuC"
                     /locus_tag="ACHXOL_001564"
     CDS             2186..3613
                     /gene="rmuC"
                     /locus_tag="ACHXOL_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR6808573.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3708..4463
                     /gene="ubiE"
                     /locus_tag="ACHXOL_001565"
     CDS             3708..4463
                     /gene="ubiE"
                     /locus_tag="ACHXOL_001565"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR6808574.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4477..5082
                     /gene="ubiJ"
                     /locus_tag="ACHXOL_001566"
     CDS             4477..5082
                     /gene="ubiJ"
                     /locus_tag="ACHXOL_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR6808575.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5079..6719
                     /gene="ubiB"
                     /locus_tag="ACHXOL_001567"
     CDS             5079..6719
                     /gene="ubiB"
                     /locus_tag="ACHXOL_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR6808576.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6798..7067
                     /gene="tatA"
                     /locus_tag="ACHXOL_001568"
     CDS             6798..7067
                     /gene="tatA"
                     /locus_tag="ACHXOL_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR6808577.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7071..7586
                     /gene="tatB"
                     /locus_tag="ACHXOL_001569"
     CDS             7071..7586
                     /gene="tatB"
                     /locus_tag="ACHXOL_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR6808578.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7589..8365
                     /gene="tatC"
                     /locus_tag="ACHXOL_001570"
     CDS             7589..8365
                     /gene="tatC"
                     /locus_tag="ACHXOL_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR6808579.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8407..9189
                     /gene="tatD"
                     /locus_tag="ACHXOL_001571"
     CDS             8407..9189
                     /gene="tatD"
                     /locus_tag="ACHXOL_001571"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR6808580.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(9186..9674)
                     /gene="rfaH"
                     /locus_tag="ACHXOL_001572"
     CDS             complement(9186..9674)
                     /gene="rfaH"
                     /locus_tag="ACHXOL_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR6808581.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9841..11334
                     /gene="ubiD"
                     /locus_tag="ACHXOL_001573"
     CDS             9841..11334
                     /gene="ubiD"
                     /locus_tag="ACHXOL_001573"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR6808582.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11381..12082
                     /gene="fre"
                     /locus_tag="ACHXOL_001574"
     CDS             11381..12082
                     /gene="fre"
                     /locus_tag="ACHXOL_001574"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR6808583.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12272..13435)
                     /gene="fadA"
                     /locus_tag="ACHXOL_001575"
     CDS             complement(12272..13435)
                     /gene="fadA"
                     /locus_tag="ACHXOL_001575"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR6808584.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13445..15634)
                     /gene="fadB"
                     /locus_tag="ACHXOL_001576"
     CDS             complement(13445..15634)
                     /gene="fadB"
                     /locus_tag="ACHXOL_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR6808585.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLSMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15824..17155
                     /gene="pepQ"
                     /locus_tag="ACHXOL_001577"
     CDS             15824..17155
                     /gene="pepQ"
                     /locus_tag="ACHXOL_001577"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR6808586.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17155..17769
                     /locus_tag="ACHXOL_001578"
     CDS             17155..17769
                     /locus_tag="ACHXOL_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR6808587.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17808..19259
                     /gene="trkH"
                     /locus_tag="ACHXOL_001579"
     CDS             17808..19259
                     /gene="trkH"
                     /locus_tag="ACHXOL_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR6808588.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19271..19816
                     /gene="hemG"
                     /locus_tag="ACHXOL_001580"
     CDS             19271..19816
                     /gene="hemG"
                     /locus_tag="ACHXOL_001580"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR6808589.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
BASE COUNT         4705 a         5106 c         5486 g         4849 t
ORIGIN      
        1 tgtttgtttg tgaatgggag ttgatggtaa atctctaagg tgtcttccat tgaagagact
       61 catttatgac tatgacttgc cgactttctt aaggtcaaat agcggagtcg taatctccaa
      121 tcaattcata ctgattattt tttgggggac agagatggca gcaaaacgca ctcgtttact
      181 caccatctca aatatagact aagaggaaac taactccgct tctctccata ctgcttaaac
      241 cattccagca tacgctgcca gccatctttc gcagattcgg catgatagct cgggcgataa
      301 tcagcgttga atgcatgccc ggcatccggg tacacgataa tctctgcttt cgcattagca
      361 gcccgcagcg cctggcgcat ggtttcaacg ctctcctgcg gaatgctgtt atcctgaccg
      421 ccatataagc cgagaatcgg cgcgttaaga tcggttgcga tatcaacagg ttgtttcggt
      481 gaattcagcg acttgtcgcc cgtcagtttg ccgtaccacg ccactgcagc ttttagctgt
      541 ggattatgcg cggcatacag ccaggtgata cgtccacccc agcagaatcc ggtgatcatt
      601 aaacgatgaa catcgccgcc gttgcgggaa gcccaactgg cggcatgatc gagatcggcc
      661 agcacctgcg agtcaggcac ttttgctacc agaccgctaa gcaacgtggg gatatcggca
      721 aaatcattcg gatcgccttc gcggaagtaa agttcaggtg cgatagccag atacccctcc
      781 agcgccagac ggcgacaaat gtcgcggata tgttcatgca cgccaaaaat ttcctgcact
      841 acaatgacca ctggcagtgg gccatcgctt tgctttggtc tggcatggta agcaggcatg
      901 ttatcccctt gtgaagggat agaggtgaag cccgccacaa tcgcgtcgtc cagggtctga
      961 acgatggtcg aagcgagagg cgatgcagca ggtgcaaatc cagattgttg tgttgttgcc
     1021 atggtattct ccgtaccctt ataaaaatgt tgcgcaatgt taactatagt cagcatgcaa
     1081 caaatcacat tgcctgaatc ggctcatctt ttatgcagtc ctgcagaatg aagagtgatt
     1141 tatgtgattt gaatcacttt tggtgggtaa atttatgcaa cgcatttgcg tcatggtgat
     1201 gagtatcacg aaaaaatgtt aaacccttcg gtaaagtgtc tttttgcttc ttctgactaa
     1261 accgattcac agaggagttg tatatgtcca agtctgatgt ttttcatctc ggcctcacta
     1321 aaaacgattt acaaggggct acgcttgcca tcgtccctgg cgacccggat cgtgtggaaa
     1381 agatcgccgc gctgatggat aagccggtta agctggcatc tcaccgcgaa ttcactacct
     1441 ggcgtgcaga gctggatggt aaacctgtta tcgtctgctc taccggtatc ggcggcccgt
     1501 ctacctctat tgctgttgaa gagctggcac agctgggcat tcgcaccttc ctgcgtatcg
     1561 gtacaacggg cgctattcag ccgcatatta atgtgggtga tgtcctggtt accacggcgt
     1621 ctgtccgtct ggatggcgcg agcctgcact tcgcaccgct ggaattcccg gctgtcgctg
     1681 atttcgaatg tacgactgcg ctggttgaag ctgcgaaatc cattggcgcg acaactcacg
     1741 ttggcgtgac agcttcttct gataccttct acccaggtca ggaacgttac gatacttact
     1801 ctggtcgcgt agttcgtcac tttaaaggtt ctatggaaga gtggcaggcg atgggcataa
     1861 tgaactatga aatggaatct gcaaccctgc tgaccatgtg tgcaagtcag ggcctgcgtg
     1921 ccggtatggt agcgggtgtt atcgttaacc gcacccagca agagatcccg aatgctgaga
     1981 cgatgaaaca aactgaaagc caagcggtga aaatcgtggt ggaagcggcg cgtcgtctgc
     2041 tgtaattctc ttctcctgtc tgaaggccga cgcgttcggc cttttgtatt tttgcgtagc
     2101 gcctcgcagg aaatgccttt ccaactggac gtttgtacag cacaattcta ttttgtgcgg
     2161 gtaagttgtt gcgtcaggag gcgttgtgga tttctcaatc atggtttacg cagttattgc
     2221 gttggtgggt gtggcaattg gctggctgtt tgccagttat caacatgcgc agcaaaaagc
     2281 cgagcaatta gctgaacgtg aagagatggt cgcggagtta agcgcggcaa aacaacaaat
     2341 tacccaaagc gagcactggc gtgcagagtg cgagttactc aataacgaag tgcgcagcct
     2401 gcaaagtatt aacacctctc tggaggccga tctgcgtgaa gtaaccacgc ggatggaagc
     2461 cgcacagcaa catgctgacg ataaaattcg ccagatgatt aacagcgagc agcgcctcag
     2521 tgagcagttt gaaaacctcg ccaaccgtat ttttgagcac agcaatcgcc gggttgatga
     2581 gcaaaaccgt cagagtctga acagcctgtt gtcgccgcta cgtgaacaac tggacggttt
     2641 ccgccgtcag gttcaggaca gcttcggtaa agaagcacaa gaacgccata ccctgaccca
     2701 cgaaattcgc aatctccagc aactcaacgc gcaaatggcc caggaagcga tcaacctgac
     2761 gcgcgcgctg aaaggcgaca ataaaaccca gggcaactgg ggcgaggtag tattgacgcg
     2821 ggtgctggag gcttccggtc tgcgtgaagg gtatgaatat gaaacccagg tcagcatcga
     2881 aaatgacgcc cgctcgcgga tgcagccgga tgtcatcgtg cggctgccgc agggaaaaga
     2941 tgtggtgatc gacgccaaaa tgacgctggt cgcctatgaa cgctatttta acgccgaaga
     3001 cgactacacc cgcgaaagcg cgctacagga acatatcgcg tcggtgcgta accatatccg
     3061 tttgctggga cgcaaagatt atcaacagct gccggggctg cgaactctgg attacgtgct
     3121 gatgtttatt cccgttgaac ccgctttttt actggcgctt gaccgccagc cggagctgat
     3181 caccgaagcg ttgaaaaaca atatcatgct ggttagcccg actacgctgc tggtggcgtt
     3241 gcgtactatt gccaacctgt ggcgttatga gcatcaaagc cgcaacgccc agcaaatcgc
     3301 cgatcgtgcc agcaagctgt acgacaagat gcgtttgttc atcgatgaca tgtccgcgat
     3361 tggtcaaagt ctcgacaaag cgcaggataa ctaccgccag gcaatgaaaa aactctcttc
     3421 aggtcgcgga aatgtgctgg cgcaggcaga agcgtttcgc ggtttaggag tagaaattaa
     3481 acgcgagatt aatccggatt tggctgaaca ggcggtgagc cagaatgaag agtatcgact
     3541 tcggtcggtt ccggagcagc cgaatgatga agcttatcaa cgcgatgatg aatataatca
     3601 gcagtcgcgc tagcccatta ggggtagtta agccgggtag aaatctaggg catcgacgcc
     3661 caatctgtta cacttctgga acaatttttt gatgagcagg cattgagatg gtggataagt
     3721 cacaagaaac gacgcacttt ggttttcaga ccgtcgcgaa ggaacaaaaa gcggatatgg
     3781 tcgcccacgt tttccattcc gtggcatcaa aatacgatgt catgaatgat ttgatgtcat
     3841 ttggtattca tcgtttgtgg aagcgattca cgattgattg cagcggcgta cgccgtgggc
     3901 agaccgtgct ggatctggct ggtggcaccg gcgacctgac agcgaaattc tcccgcctgg
     3961 tcggagaaac tggcaaagtg gtccttgctg atatcaatga atccatgctc aaaatgggac
     4021 gcgagaagct gcgtaatatc ggtgtgattg gcaacgttga gtatgttcag gcgaacgctg
     4081 aggcgctgcc gttcccggat aacacctttg attgcatcac catttcgttt ggtctgcgta
     4141 acgtcaccga caaagataaa gcactgcgtt caatgtatcg cgtgctgaaa cccggcggcc
     4201 gcctgctggt gcttgagttc tcgaagccaa ttatcgagcc gctgagcaaa gcctatgatg
     4261 catactcctt ccatgtgctg ccgcgtattg gctcactggt cgcgaacgac gccgacagct
     4321 accgttatct ggcagaatcc atccgtatgc atcccgatca ggataccctg aaagccatga
     4381 tgcaggatgc cggattcgaa agtgtcgact actacaatct gacggcaggg gttgtggcgc
     4441 tgcatcgtgg ttataagttc tgacaggaga ccggaaatgc cttttaaacc tttagtgacg
     4501 gcaggaattg aaagtctgct caacaccttc ctgtatcgct cacccgcgct gaaaacagcc
     4561 cgctcgcgtc tgctgggtaa agtattgcgc gtggaggtaa aaggcttttc gacgtcattg
     4621 attctggtgt tcagcgaacg ccaggttgat gtactgggcg aatgggcagg cgatgctgac
     4681 tgcaccgtta tcgcctacgc cagtgtgttg ccgaaacttc gcgatcgcca gcagcttacc
     4741 gcactgattc gcagtggtga gctggaagtg cagggcgata ttcaggtggt gcaaaacttc
     4801 gttgcgctgg cagatctggc agagttcgac cctgcggaac tgctggcccc ttataccggt
     4861 gatatcgccg ctgaaggaat cagcaaagcc atgcgcggag gcgcaaagtt cctgcatcac
     4921 ggcattaagc gccagcaacg ttatgtggcg gaagccatta ctgaagagtg gcgtatggca
     4981 cccggtccgc ttgaagtggc ctggtttgcg gaagagacgg ctgccgtcga gcgtgctgtt
     5041 gatgccctga ccaaacggct ggaaaaactg gaggctaaat gacgccaggt gaagtacggc
     5101 gcctatattt catcattcgc acttttttaa gctacggact tgatgaactg atccccaaaa
     5161 tgcgtatcac cctgccgcta cggctatggc gatactcatt attctggatg ccaaatcggc
     5221 ataaagacaa acttttaggt gagcgactac gactggccct gcaagaactg gggccggttt
     5281 ggatcaagtt cgggcaaatg ttatcaaccc gccgcgatct ttttccaccg catattgccg
     5341 atcagctggc gttattgcag gacaaagttg ctccgtttga tggcaagctg gcgaagcagc
     5401 agattgaagc tgcaatgggc ggcttgccgg tagaagcgtg gtttgacgat tttgaaatca
     5461 agccactggc ttctgcttct atcgcccagg ttcataccgc gcgattgaaa tcgaatggta
     5521 aagaggtggt gattaaagtc atccgcccgg atattttgcc ggttattaaa gcggatctga
     5581 aacttatcta ccgtctggct cgctgggtgc cgcgtttgct gccggatggt cgccgtctgc
     5641 gcccaaccga agtggtgcgc gagtacgaaa agaccttgat tgatgaactg aatttgctgc
     5701 gggaatctgc caacgccatt cagcttcggc gaaattttga agacagcccg atgctctaca
     5761 tcccggaagt ttaccctgac tattgtagtg aagggatgat ggtgatggag cgcatttacg
     5821 gcattccggt gtctgatgtt gcgacgctgg agaaaaatgg caccaacatg aaattgctgg
     5881 cggaacgtgg cgtgcaggtg ttcttcactc aggtctttcg cgacagcttt ttccatgccg
     5941 atatgcaccc tggcaacatt ttcgtaagct atgaacaccc ggaaaacccg aaatatatcg
     6001 gcattgattg cgggattgtt ggctcgctaa acaaagaaga taaacgctat ctggcagaaa
     6061 actttatcgc cttctttaat cgcgactatc gcaaagtggc agagctacac gtcgattctg
     6121 gctgggtgcc accagatacc aacgttgaag agttcgaatt tgccattcgt acggtctgtg
     6181 aacctatctt tgagaaaccg ctggccgaaa tttcgtttgg acatgtactg ttaaatctgt
     6241 ttaatacggc gcgtcgcttc aatatggaag tgcagccgca actggtgtta ctccagaaaa
     6301 ccctgctcta tgtcgaaggg gtaggacgcc agctttatcc gcagctcgat ttatggaaaa
     6361 cagcgaagcc tttcctggag tcgtggatta aagatcaggt cggtattcct gccctggtga
     6421 gagcatttaa agaaaaagcg ccgttctggg tcgaaaaaat gccagaactg cctgaattgg
     6481 tttacgacag tttgcgccag ggcaagtatt tacagcacag tgttgataag attgcccgcg
     6541 agcttcagtc aaatcatgta cgtcagggac aatcgcgtta ttttctcgga attggcgcta
     6601 cgttagtatt aagtggcaca ttcttgttgg tcagccgacc tgaatggggg ctgatgcccg
     6661 gctggttaat ggcaggtggt ctgatcgcct ggtttgtcgg ttggcgcaaa acacgctgat
     6721 tttttcatcg ctcaaggcgg gtcgtgtaac gtataatgcg gccttgttta atcatcatct
     6781 accacagagg aacatgtatg ggtggtatca gtatttggca gttattgatt attgccgtca
     6841 tcgttgtact gctttttggc accaaaaagc tcggctccat cggttccgat cttggtgcgt
     6901 cgatcaaagg ctttaaaaaa gcaatgagcg atgatgaacc aaagcaggat aaaaccagtc
     6961 aggatgctga ttttactgcg aaaactatcg ccgataagca ggcggatacg aatcaggaac
     7021 aggctaaaac agaagacgcg aagcgccacg ataaagagca ggtgtaatcc gtgtttgata
     7081 tcggttttag cgaactgcta ttggtgttca tcatcggcct cgtcgttctg gggccgcaac
     7141 gactgcctgt ggcggtaaaa acggtagcgg gctggattcg cgcgttgcgt tcactggcga
     7201 caacggtgca gaacgaactg acccaggagt taaaactcca ggagtttcag gacagtctga
     7261 aaaaggttga aaaggcgagc ctcactaacc tgacgcccga actgaaagcg tcgatggatg
     7321 aactacgcca ggccgcggag tcgatgaagc gttcctacgt tgcaaacgat cctgaaaagg
     7381 cgagcgatga agcgcacacc atccataacc cggtggtgaa agataatgaa gctgcgcatg
     7441 agggcgtaac gcctgccgct gcacaaacgc aggccagttc gccggaacag aagccagaaa
     7501 ccacgccaga gccggtggta aaacctgctg cggacgctga accgaaaacc gctgcacctt
     7561 ccccttcgtc gagtgataaa ccgtaaacat gtctgtagaa gatactcaac cgcttatcac
     7621 gcatctaatt gagctgcgta agcgtctgct gaactgcatt atcgcggtga tcgtgatatt
     7681 cctgtgtctg gtctatttcg ccaatgacat ctatcacctg gtatccgcgc cattgatcaa
     7741 gcagttgccg caaggttcaa cgatgatcgc caccgacgtg gcctcgccgt tctttacgcc
     7801 gatcaagctg acctttatgg tgtcgctgat tctgtcagcg ccggtgattc tctatcaggt
     7861 gtgggcattt atcgccccag cgctgtataa gcatgaacgt cgcctggtgg tgccgctgct
     7921 ggtttccagc tctctgctgt tttatatcgg catggcattc gcctactttg tggtctttcc
     7981 gctggcattt ggcttccttg ccaataccgc gccggaaggg gtgcaggtat ccaccgacat
     8041 cgccagctat ttaagcttcg ttatggcgct gtttatggcg tttggtgtct cctttgaagt
     8101 gccggtggca attgtgctgc tgtgctggat ggggattacc tcgccagaag acttacgcaa
     8161 aaaacgcccg tatgtgctgg ttggtgcatt cgttgtcggg atgttgctga cgccgccgga
     8221 tgtcttctcg caaacgctat tggcgatccc gatgtactgt ctgtttgaaa tcggtgtctt
     8281 cttctcacgc ttttacgttg gtaaagggcg aaatcgggaa gaggaaaacg acgctgaagc
     8341 agaaagcgaa aaaactgaag aataaattca accgcccgtc agggcggttg tcatatggag
     8401 tacaggatgt ttgatatcgg cgttaatttg accagttcgc aatttgcgaa agaccgtgat
     8461 gatgttgtag cgcgcgcttt tgacgcggga gttaatgggc tactcatcac cggcactaac
     8521 ctgcgtgaaa gccagcaggc gcaaaagctg gcgcgtcagt attcgtcctg ttggtcaacg
     8581 gcgggcgtac atcctcacga cagcagccag tggcaagctg cgactgaaga agcgattatt
     8641 gagctggccg cgcagccaga agtggtggcg attggtgaat gtggtctcga ctttaaccgc
     8701 aacttttcga cgccggaaga gcaggaacgc gcttttgttg cccagctacg cattgccgca
     8761 gatttaaaca tgccggtatt tatgcactgt cgcgatgccc acgagcggtt tatgacattg
     8821 ctggagccgt ggctggataa actgcctggt gcggttcttc attgctttac cggcacacgc
     8881 gaagagatgc aggcgtgcgt ggcgcatgga atttatatcg gcattaccgg ttgggtttac
     8941 gatgaacgac gcggactgga gctgcgggaa cttttgccgt tgattccggc ggaaaaatta
     9001 ctgatcgaaa ctgatgcgcc gtatctgctc cctcgcgatc tcacgccaaa gccatcatcc
     9061 cggcgcaacg agccagccca tctgccccat attttgcaac gtattgcgca ctggcgtgga
     9121 gaagatgccg catggctggc tgccaccacg gatgctaatg tcaaaacacg gtttgggatt
     9181 gcgttttaga gtttgcggaa ctcggtgttc ttcacactgt gcttaatctc tttattaata
     9241 agattaagca atagcatgga gcgagcctca ccatcgggtt cggtgaaaat ggcctgaaag
     9301 ccttcgaacg cgccttcggt aataatcacc ttatctcccg gataaggggt tgccggatcg
     9361 acaatgtctt tcggtttata taccgatagc tgatgaatca ccgccgatgg gactatcgct
     9421 agcgacgcgc caaagcgcac gaagtggctg acaccgcggg tcgcgttgat agtcgtggta
     9481 tgaatcactt ctggatcaaa ttcgacaaac aggtagttgg gaaacaatgg ctcactgact
     9541 gcagtacgtt ttccacgcac gattttttcc agggtgatca tcggtgccag gcaattcaca
     9601 gcctgtctct caaggtgttc ctgggcacgt tgaagttgcc cgcgcttgca gtacagtaaa
     9661 taccaagatt gcataatgac tcttatccgt ttaatcgggg cgcaaggata gcaaaagctt
     9721 tacgctaagt taattatatt ccccggtttg cgttataccg tcagagttca cgctaattta
     9781 acaaatttac agcatcgcaa agatgaacgc cgtataatgg gcgtagatta agaggctaca
     9841 atggacgcca tgaaatataa cgatttacgc gacttcttga cgctgcttga acagcagggt
     9901 gagctaaaac gtatcacgct cccggtggat ccgcatctgg aaatcactga aattgctgac
     9961 cgcactttgc gtgccggtgg gcctgcgctg ttgttcgaaa accctaaagg ctactcaatg
    10021 ccggtgctgt gcaacctgtt cggtacgcca aagcgcgtgg cgatgggcat ggggcaggaa
    10081 gatgtttcgg cgctgcgtga agttggtaaa ttattggcgt ttctgaaaga gcccgagccg
    10141 ccaaaaggtt tccgcgacct gtttgataaa ctgccgcagt ttaagcaagt attgaacatg
    10201 ccgacaaagc ggctgcgtgg tgcgccctgc caacaaaaaa tcgtctctgg cgatgacgtc
    10261 gatctcaatc gcattcccat tatgacctgc tggccggaag atgccgcgcc gctgattacc
    10321 tgggggctga ccgtaacgcg cggcccgcat aaagagcggc agaatctggg catttatcgc
    10381 cagcagctga ttggtaaaaa caaactgatt atgcgctggc tgtcgcatcg cggcggcgcg
    10441 ctggattatc aggagtggtg tgcggcgcat ccgggcgaac gtttcccggt ttctgtggcg
    10501 ctgggtgccg atcccgccac gattctcggt gcagtcaccc ccgttccgga tacactttca
    10561 gagtatgcgt ttgccggatt gctgcgcggt accaagaccg aagtggtgaa gtgtatctcc
    10621 aatgaccttg aagtgcccgc cagtgcggag attgtgctgg aagggtatat cgaacaaggc
    10681 gaaactgcgc cggaagggcc gtatggcgac cacaccggtt actataacga agtcgatagt
    10741 ttcccggtat ttaccgtgac gcatattacc cagcgtgaag atgcgattta ccactccacc
    10801 tatactggtc gtccgccgga tgaacccgcg gtgctgggtg tcgcactgaa cgaagtgttt
    10861 gtgccgattc tgcaaaaaca gttcccggaa attgtcgatt tttacctgcc accggaaggc
    10921 tgttcttatc gtctggcggt agtgacaatc aaaaaacagt acgccggaca cgcgaagcgc
    10981 gtcatgatgg gcgtctggtc gttcttacgc cagtttatgt acactaaatt tgtgatcgtt
    11041 tgcgatgatg acgtcaacgc acgcgactgg aacgatgtga tttgggcgat taccacccgt
    11101 atggacccgg cgcgggatac tgttctggta gaaaatacgc ctattgatta tctggatttt
    11161 gcctcgcctg tctccgggct gggttcaaaa atggggctgg atgccacgaa taaatggccg
    11221 ggggaaaccc agcgtgaatg gggacgtccc atcaaaaaag atcctgatgt cgtcgcgcat
    11281 attgacgcca tctgggatga actggctatt tttaacaacg gtaaaagcgc ctgatgcgcg
    11341 ttagttttgc cctatttatt tgatccgaca gagaaagcgc atgacaacct taagctgtaa
    11401 agtgacctcg gtagaagcta tcacggatac tgtataccgt gtccgaatcg taccagacgc
    11461 ggccttttct tttcgtgctg gtcagtattt gatggtagtg atggatgagc gcgacaaacg
    11521 tccgttttca atggcttcga cgccggatga aaaagggttt atcgagctgc atattggcgc
    11581 ttctgaaatc aacctttacg cgaaagcagt catggaccgc atcctcaaag atcatcaaat
    11641 cgtggtcgat attccccacg gagaagcgtg gctgcgcgat gatgaagagc gtccgatgat
    11701 tttgattgcg ggcggcaccg ggttctccta tgcgcgctcg attttattga cggcgttggc
    11761 gcgtaatccc aatcgcgata tcaccattta ctggggtggg cgtgaagagc aacatctgta
    11821 tgatctctgc gagcttgagg cgctttcgct gaagcatcct ggtctgcaag tggtgccggt
    11881 ggttgaacaa ccggaagcgg gctggcgtgg gcgtactggc accgtgttaa cggcggtctt
    11941 gcaggatcat ggtacgctgg cagagcatga tatctatatt gccggacgtt ttgagatggc
    12001 gaaaattgct cgtgacctgt tttgcagtga gcgtaatgcg cgggaagatc gtctgtttgg
    12061 cgatgcgttt gcatttatct gagatataaa aacccgcccc tgacaggcgg gaagaacggc
    12121 aactaaaact ttattcagtg gcatttagat ctatgtcgca tccggcaatc ggtgcacgat
    12181 gcctgatgcg acgctggcgc gtcttatcag gcctacattg gtgcaggatc ggtagaccgg
    12241 ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg ctcaaacacc gtcgcaatac
    12301 cctgacccag accgatacac atcgtcgcca gaccaaactg aacgtctttg tgttccatca
    12361 gattcagcaa cgtggtgctg atacgcgcac cggaacaacc cagcggatga cccagcgcga
    12421 tcgcaccacc attgaggttg atcttctcgt caatctgctc cattagcccg agatctttaa
    12481 tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc aaacacgccg atatcgctgg
    12541 cagaaagccc cgcttttttc agcgccagct tcgaggcagg aaccgggccg taacccataa
    12601 tcgacggatc acaaccaacg accgccatcg aacgcacacg ggcgcgcggc ttaagaccta
    12661 attcacgggc gcggctttca ctcatcacca gcatggccgc ggcaccatca gaaagtgcag
    12721 aagatgtacc cgccgttacc gtaccgctta ctggatcaaa cgccggacgc agcgtggcga
    12781 gggcttccac ggtggtttcc gggcgaatca cttcgtcgta attaaactgc ttcaggacgc
    12841 cgtcggcatc gtgaccaccg gtcgggatga tttcattttt aaatgcgccc gactgcgtgg
    12901 cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc ctgcatttca cggctgatac
    12961 cgtgcatacg cgccagcatt tctgccgtta agcccatcat gcccgccgct ttggcgacat
    13021 tgcggctcag gccggggtga aaatcgacgc cgtgactcat cggcacatgg cccatatgct
    13081 ccacgccgcc aaccagacat gcctgcgcat cgccagtcat gatcattcgt gctgcgtcat
    13141 gcagtgcctg catggatgaa ccacacaagc gattgacggt aaccgccggg acagagtgcg
    13201 gcacttctgc cagcagcgcc gcgttacggg cgatattaaa accctgctcc agcgtctgct
    13261 gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc cagcgccggg ttacgcgcca
    13321 gcaggctacg cattaaatga gcggagagat cttctgcacg cacgttacga aaagcaccgc
    13381 ccttcgaacg gcccatcggg gtgcgaattg catcgacaat gacaacctgt tccattgtga
    13441 ctccttaagc cgttttcagg tcgccaaccg gacgggctgg ctcaaccgga ggatagtacg
    13501 gttcgttatg acgcgcttta ttacgcagac cttccggcac ttcatacagc gggccgaggt
    13561 gctgatattg ctgtgccata tcgaggtatt ttgcgctacc gagggtgtcc agccagcgga
    13621 acgcgccgcc gtggaacgga gggaagccca ggccgtagac cagcgccata tccgcttccg
    13681 ccggagtggc gataatgccc tcttccagac agcgcaccac ttcgttgacc atcgggatca
    13741 tcatgcgggc gataatctct tcttcgctga aatcgcgctt cggctggctc acttctgcca
    13801 gcaggtcttc aacggcggcg tcttcttctt tcttcggctt acctttgctg tcttctttat
    13861 aacgccagaa accgaggccg ttcttctgac caaagcggtt ggcatcaaac agcgcgtcga
    13921 tggcatcgcg gtaatctttt tgcatccgct gcgggaagcc tgctgccatg acagcctgag
    13981 cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata tgccgggccc atcggccagc
    14041 caaactgttt ttccatcact ttgtcgatct tgcggaaatc cgcgccgtcg cgcagcagct
    14101 ggctgaaacc ggcgaaatac gggaacagca cgcggttaac aaagaagccg gggcagtcgt
    14161 taaccacaat cggcgtcttg cccatcttgc tcgcccaggc gacaactttc gcgatggttt
    14221 cgtcggagct tttctcgccg cgaataattt ctaccaacgg cattcggtgg accgggttaa
    14281 agaagtgcat cccgcagaag ttttccgggc gttccagcgc gttggccagt tcgctgatag
    14341 gaatggttga agtgttagac gccagcacgg tatcctggcg tactttttgt tcggtttctg
    14401 ccagtacggc ttttttcact ttcgggtttt caacaaccgc ttctaccaca atatccacgc
    14461 ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga gatcacgcca gccagtttca
    14521 gaccatcgat cttgccgcgc tcaagctgct tgttcagcag tttcgcggct tcggtcatgc
    14581 tgagggttaa cgacttgtcg ttgatatctt tcatgacaac cggcacgcct ttccacgcgg
    14641 actggtaagc gataccgcca cccatgatgc ctgcgcccag cacagcagcc tgtttcgggg
    14701 tttcaacgtc tttggtgagt ttcttcgctt tgccttttac atattgatcg ttaaggaaaa
    14761 tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg gacaaaactt ttgttttcca
    14821 gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc aatggttttt actgcggtga
    14881 tgggggccgg ataatgtttc cccgccgttt gcgcgaccat tcctttagcg atggtgaagc
    14941 tcatggtggc ttcaatcttg ctcagtttta gcggttccag cttcggctgg cgtttcgctt
    15001 tccagtcgag gtcgccgtta atggcctggc gtaaaaccgc ctttgcgcct tcaaccagtt
    15061 tttctgcttt gactacccca tccaccagac cgattttcag cgcctgatcc gcgccgacat
    15121 ctttaccggc ggcaatgatt tccagcgcac tgtcagcgcc cagcatacgt ggcatacgta
    15181 cagaaccgcc aaagcccggc atgatgccca gtttggtttc cggcagaccg atgcgcagat
    15241 ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc gcagccacca cccagcgcat
    15301 agccgttgac ggcagcaatg gtcggcaccg gcagatcttc caggcgatta aacacgctat
    15361 tggcaaagtg cagccactga cttaactgtt cttcaggaac gaggaacagg gacaaaaatt
    15421 cggtgatatc agcaccgacg ataaaggctg ctttgttcga acgcagcagc agccctttta
    15481 gatctgattg ctgttccagc acgccgatgg cctcgccgag gctggcgacg gtcgcagtgt
    15541 cgagtttatt aactgaacct ggggcatcaa ataccagttc ggcaatgcca tcttccagcc
    15601 agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt ctcctgaatc cgcaaggtga
    15661 tctggtcgta ccagatgcat cgaagtgtgt attttgtgtt aaaaatatgc aaataaaaga
    15721 ttaaagaaat gccgatctga tcacgctcgg cagaaatcac gctctggatg aacgatgtgc
    15781 taagatgcgg agacttaagg tcaaaaaaac agaagggtaa aaaatggaat cactggcctc
    15841 gctctataaa aatcatatag ctaccttaca agaacggact cgcgatgcgc tggcgcgctt
    15901 caagctggat gcattactta ttcactccgg cgagctgttc aacgtttttc tcgacgatca
    15961 tccctatccg tttaaagtga acccgcaatt caaagcgtgg gtgccggtaa ctcaggtgcc
    16021 aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa ctgtggttct atctgccggt
    16081 tgattactgg cacaacgtcg aaccgctgcc gacctccttc tggactgaag atgtggaagt
    16141 gatcgcactg ccgaaagccg atggcattgg tagcctgctg cccgctgcgc gcggcaatat
    16201 cggttatatc ggtccggtgc cggaacgtgc gctgcaactg ggtattgagg ccagcaacat
    16261 caacccgaaa ggggtgatcg actacctgca ttactaccgc tccttcaaaa ccgagtacga
    16321 actggcctgt atgcgtgaag cgcagaaaat ggcggtcaac ggtcatcgtg cggcagaaga
    16381 agcgttccgt tctggcatga gcgagttcga tatcaacatc gcctatctga ccgcgaccgg
    16441 tcatcgtgat accgacgtac cttacagcaa cattgtggcg cttaacgaac acgcttcggt
    16501 gctgcattac accaaactgg atcatcaggc accggaagag atgcgcagct tcctgctgga
    16561 tgccggggcc gaatataacg gctatgcggc tgacctgact cgtacctggt cggcaaaaag
    16621 cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat gaacaactgg cgctgatcgc
    16681 caccatgaaa gcaggcgtta gctatgtgga ttaccacatc cagttccatc agcgcatcgc
    16741 caaactgctg cgtaaacatc aaatcatcac cgatatgagt gaagaagcga tggtcgaaaa
    16801 cgatctcacc ggaccgttta tgccgcatgg tatcggtcat ccgctgggcc tgcaggtgca
    16861 tgacgtagcc ggttttatgc aggatgatag cggtacacac ctcgcggcac cggcaaaata
    16921 tccgtacctg cgctgcaccc gtattctcca gccgggtatg gtgttaacca tcgaaccggg
    16981 tatctacttc attgaatcgc tactggcacc gtggcgtgaa gggcagttca gcaagcactt
    17041 caactggcag aaaattgaag cactgaaacc gttcggcggc attcgtatcg aagacaacgt
    17101 ggtgatccac gaaaacaacg tggaaaacat gacccgggat ctgaaactgg cgtgatggaa
    17161 agctggttaa ttcctgcggc accggtcacg gtcgttgaag agatcaaaaa aagccgcttc
    17221 ataacgatgt tggcgcatac cgatggcgtt gaggcggcga aagcgtttgt tgaatcggtg
    17281 cgggcagaac accccgatgc ccgccaccat tgcgtggcgt gggtggcggg tgcgccggat
    17341 gattctcaac agctgggttt ctctgacgac ggggagccgg caggaacggc aggtaaaccg
    17401 atgctcgccc agctaatggg cagcggcgtc ggggaaatta ccgctgtggt agtgcgctac
    17461 tacggcggca tattgcttgg caccggtggg ttagtgaaag cgtatggcgg cggcgtgaat
    17521 caggcgctgc gccagctaac gacccaacgc aagacgccat taaccgaata tactttgcaa
    17581 tgtgaatatc atcagttaac cggcattgaa gcgttgctgg ggcagtgtga cggcaaaatt
    17641 atcaacagtg attatcaggc attcgttctg ctgcgggtgg cgcttccggc ggcgaaagtg
    17701 gctgaatttt ccgcaaagct ggcggatttt agccgtggtt cattgcaatt gttagcgatt
    17761 gaagaataat ccccacttcg ttttgcagaa ctaaggaagc ggcagagatg cattttcgcg
    17821 ccattacccg aatcgttgga ctactggtca tcttattttc agggaccatg attatccctg
    17881 ggctggtagc actcatctac cgggatggag cgggccgcgc ttttacccag accttttttg
    17941 tcgccctcgc cattggttct atgctgtggt ggccgaaccg taaagagaag ggcgaactga
    18001 aatcccgtga ggggtttctg atagtggtgc tgttctggac cgtgctgggt agtgtcggtg
    18061 cgctcccttt tatcttctcg gaaagcccga acctcacgat taccgatgcg ttttttgaat
    18121 ctttctctgg cctgaccacc accggggcca ctacgctggt ggggctggat tcgctccctc
    18181 atgccatcct cttttatcgc cagatgctgc aatggtttgg cgggatgggg atcatcgtgt
    18241 tagcggttgc gatactgcct atcctcggcg tgggtgggat gcagctctat cgcgcagaaa
    18301 tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat tgcggaaacg gcgaaaaccc
    18361 tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct ggcgttgtgg tttgccggaa
    18421 tggatgcctt tgatgccatc ggccatagct ttgcgactat cgctattggc ggcttctcga
    18481 cacatgatgc cagtatcggt tatttcgaca gcccgactat taacactatc attgctatct
    18541 tcctgctgat ctccggctgt aactacggtc tgcacttttc actgttaagt gggcgtagtc
    18601 tgaaggttta ttggcgcgat ccggaatttc gcatgtttat cggcgtacag tttacgctgg
    18661 tggttatttg tacactcgta ctgtggtttc ataatgtcta cagttcggcg ctgatgacaa
    18721 ttaaccaggc gtttttccag gtggtatcga tggcgacaac cgccgggttt acgactgaca
    18781 gcattgcccg ctggccgctc tttttgccgg tactgctttt atgttcagct tttatcggcg
    18841 gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg catcctgctg ctgtttaagc
    18901 aggggaaccg tgagctgaaa cgactggtgc atccgaacgc cgtctatagc attaagctgg
    18961 ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg gggatttttc tccgcctatg
    19021 cattggtgtt tattgtcagt atgctggcga ttatcgccac gggcgtggat gacttttctg
    19081 cctttgcttc agttgttgcg acattgaata acctggggcc agggcttggc gtggttgctg
    19141 ataactttac cagtatgaac ccggtggcta aatggatcct gattgccaac atgctgtttg
    19201 gtcgtctcga ggtctttaca ttgctggtgc tctttacccc gactttctgg cgtgaatgat
    19261 ggagtaatac gtgaaaacat taattctttt ctcaacaagg gacggacaaa cgcgcgagat
    19321 tgcctcctac ctggcttcgg aactgaaaga actggggatc cagacggatg tcgccaatgt
    19381 gcaccgcatt gaagaaccac aatgggaaaa ttatgaccgt gtggtcattg gtgcttctat
    19441 tcgctatggt cactaccact ctgcgttcca ggaatttgtc aaaaaacatg cgacgcgact
    19501 gaattcgatg ccgagcgcct tttattccgt aaacctggtg gcgcgcaaac cggagaagcg
    19561 tactccacag accaacagct acgcgcggaa gtttctgatg aactcgcaat ggcgtcccga
    19621 tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt tatcgctggt acgatcgttt
    19681 tatgatcaag ctaattatga agatgtcagg cggtgaaacg gatacgcgca aagaagttgt
    19741 ctataccgat tgggagcagg tggcgaattt cgcccgagaa atcgcccagt taaccaacaa
    19801 accgacgctg aaataagcat aaagaataaa aaatgcgcgg tcagaaaatt attttaaatt
    19861 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
    19921 aaacacgccg ccgggtcagc ggggttctcc tgagaattcc gacagagaaa gcaaaaataa
    19981 atgcttgact ctgtagcggg aaagcgtatt atgcacaccc cgcgccgctg agaaaaagtg
    20041 aagcggcact gctctttaac aatttatcag acaatctgtg tgggcactcg aagatacgga
    20101 ttcttaacgt cgcaagacga aaaatgaata ccaagtctca agagtg
//
LOCUS       DAZRUF010000054        20091 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZRUF010000054 DAZRUF010000000
VERSION     DAZRUF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.054"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..735
                     /gene="dosC"
                     /locus_tag="ACHXOL_001581"
     CDS             <1..735
                     /gene="dosC"
                     /locus_tag="ACHXOL_001581"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR6808590.1"
                     /translation="FGLWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLN
                     NRSLRVKFLLQIRNTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIA
                     HANRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGD
                     EFIIVLTEASENETLRTAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERL
                     IQIADEALYIAKRRGRNRVELWKASL"
     gene            760..3159
                     /gene="dosP"
                     /locus_tag="ACHXOL_001582"
                     /gene_synonym="pdeO"
     CDS             760..3159
                     /gene="dosP"
                     /locus_tag="ACHXOL_001582"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR6808591.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            3418..3999
                     /gene="ddpX"
                     /locus_tag="ACHXOL_001583"
     CDS             3418..3999
                     /gene="ddpX"
                     /locus_tag="ACHXOL_001583"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR6808592.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            4013..5563
                     /locus_tag="ACHXOL_001584"
     CDS             4013..5563
                     /locus_tag="ACHXOL_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR6808593.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            5565..6587
                     /locus_tag="ACHXOL_001585"
     CDS             5565..6587
                     /locus_tag="ACHXOL_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR6808594.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            6584..7480
                     /gene="ddpC"
                     /locus_tag="ACHXOL_001586"
     CDS             6584..7480
                     /gene="ddpC"
                     /locus_tag="ACHXOL_001586"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR6808595.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            7477..8463
                     /locus_tag="ACHXOL_001587"
     CDS             7477..8463
                     /locus_tag="ACHXOL_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR6808596.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            8492..9388
                     /locus_tag="ACHXOL_001588"
     CDS             8492..9388
                     /locus_tag="ACHXOL_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR6808597.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(9444..9875)
                     /gene="osmC"
                     /locus_tag="ACHXOL_001589"
     CDS             complement(9444..9875)
                     /gene="osmC"
                     /locus_tag="ACHXOL_001589"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR6808598.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            10220..10435
                     /gene="bdm"
                     /locus_tag="ACHXOL_001590"
     CDS             10220..10435
                     /gene="bdm"
                     /locus_tag="ACHXOL_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR6808599.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10537..10674
                     /gene="sra"
                     /locus_tag="ACHXOL_001591"
     CDS             10537..10674
                     /gene="sra"
                     /locus_tag="ACHXOL_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR6808600.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            10831..12528
                     /gene="maeA"
                     /locus_tag="ACHXOL_001592"
     CDS             10831..12528
                     /gene="maeA"
                     /locus_tag="ACHXOL_001592"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR6808601.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDLFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            12662..13664
                     /gene="adhP"
                     /locus_tag="ACHXOL_001593"
                     /pseudo
     CDS             12662..13664
                     /gene="adhP"
                     /locus_tag="ACHXOL_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            13810..14094
                     /locus_tag="ACHXOL_001594"
     CDS             13810..14094
                     /locus_tag="ACHXOL_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR6808602.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(14322..14975)
                     /gene="fdnI"
                     /locus_tag="ACHXOL_001595"
     CDS             complement(14322..14975)
                     /gene="fdnI"
                     /locus_tag="ACHXOL_001595"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR6808603.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(14968..15852)
                     /gene="fdnH"
                     /locus_tag="ACHXOL_001596"
     CDS             complement(14968..15852)
                     /gene="fdnH"
                     /locus_tag="ACHXOL_001596"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR6808604.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(15865..18912)
                     /gene="fdnG"
                     /locus_tag="ACHXOL_001597"
     CDS             complement(15865..18912)
                     /gene="fdnG"
                     /locus_tag="ACHXOL_001597"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(18325..18327),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR6808605.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            19144..20025
                     /gene="yddG"
                     /locus_tag="ACHXOL_001598"
     CDS             19144..20025
                     /gene="yddG"
                     /locus_tag="ACHXOL_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR6808606.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         4892 a         5160 c         5134 g         4905 t
ORIGIN      
        1 tttggcctgt ggtttaacca taaaggtcga cattatttta gtggcattgc cgaagtaggc
       61 catatctccc gtctcattca ggatttcgac ggtattttca atcaaaccat gcgtaacacc
      121 aggaatttga ataacagaag tctgcgggtg aaatttttat tacagataag aaataccgta
      181 tcgcagatta ttaccttgct gcgtgaattg tttgaagaag tatcgcgcca cgaagtcggt
      241 atggatgtac taacgaaatt acttaaccgc cgtttcctgc cgactatctt caaacgcgaa
      301 attgcccatg ccaaccggac cggtacaccg ctgtcagtgc tgattattga cgttgataaa
      361 ttcaaagaga tcaacgatac gtggggccat aacactggcg atgaaattct gcgtaaagtc
      421 tctcaggcct tttatgacaa cgtccacagt agtgattatg tttttcgcta cgggggcgat
      481 gaatttatca ttgttttgac tgaagcttct gaaaacgaaa cgttacgtac cgcagaacgt
      541 attcgcagtc gggtggagaa aaccaaactg aaagccgcaa acggcgagga tattgccctc
      601 tcactttcca tcggtgccgc catgtttaat ggtcatcctg actatgagcg cctcattcaa
      661 atagccgatg aagctctgta tatcgccaaa agacgaggta gaaaccgtgt tgaactctgg
      721 aaagccagtc tttagatgcg ccaggatgca gaggtaatca tgaagctaac cgatgcggaa
      781 aatgccgccg atggcatttt tttccccgcc cttgaacaaa atatgatggg cgcggtgtta
      841 attaacgaaa atgatgaagt gatgtttttc aaccccgccg cagagaagct ctgggggtac
      901 aaacgtgaag aagtcattgg caataacatt gatatgctga ttccgcggga tttgcgtcct
      961 gcgcatcctg aatacattcg tcataaccgt gaaggtggta aagcgcgcgt cgaggggatg
     1021 agtcgggagc tgcagctgga gaaaaaagac ggcagtaaaa tctggacccg ttttgcgcta
     1081 tcgaaagtga gcgccgaggg gaaagtttat tacctggcgc tggtacggga tgccagcgta
     1141 gaaatggcgc aaaaagaaca gacccgacaa ttgattattg ccgttgacca tctcgaccga
     1201 ccggtgattg tcctcgatcc ggaacgccat attgtgcagt gcaatcgcgc atttaccgaa
     1261 atgtttggtt actgcattag cgaagccagc ggtatgcagc ccgatacact cctgaacatt
     1321 cctgaattcc ctgccgataa ccgcattcgt ttacaacagt tgctatggaa aaccgcccgc
     1381 gatcaggacg aatttctgct gttgacacgc accggtgaaa aaatctggat taaagcctct
     1441 atcagcccgg tttatgacgt gctcgcgcat ctgcagaacc tggtaatgac cttctcggat
     1501 atcaccgaag aacggcagat ccgccagctt gaaggcaata ttctcgccgc catgtgcagc
     1561 agcccgccat ttcatgaaat gggggaaatc atttgtcgta acatcgaatc tgttctcaac
     1621 gaatcgcatg tttcgctgtt cgcactgcgc aacgggatgc cgatacactg ggcgtcatct
     1681 tcgcacggtg cagaagttca aaatgcgcaa agctggtcag cgaccattcg tcagcgtgat
     1741 ggtgcgcctg cggggatcct gcaaattaaa acctcgtcag gagcagaaac cagcgccttt
     1801 atcgaacgcg tggcagatat cagccagcat atggccgcgc tggcgctgga acaggaaaaa
     1861 agccgtcagc atattgaaca actcatccaa tttgatccga tgaccggtct gccaaatcgc
     1921 aataacctgc acaattacct cgatgacctt gtcgacaaag ccgtctctcc ggtggtgtat
     1981 ctcatcggtg ttgaccatat tcaggatgtg attgatagcc ttggctatgc gtgggccgat
     2041 caggcattgc tggaagtggt caatcgcttt cgtgaaaaac tcaaaccgga tcagtatctc
     2101 tgtcgtatcg aaggtacgca gtttgtcctc gtgagcctgg aaaacgacgt cagtaacatt
     2161 acccaaatcg ccgatgagct acggaatgtg gtcagcaagc cgataatgat tgacgataaa
     2221 ccattcccgc taaccttgag tattggcatc agctacgacg tgggtaaaaa ccgcgattac
     2281 ctgctctcca ctgctcacaa tgcaatggat tatattcgca agaatggcgg taacggctgg
     2341 cagttcttca gcccggcgat gaacgaaatg gtaaaagagc gtttggtttt aggcgcagcg
     2401 ctgaaagaag cgattagcaa taaccaactg aaactggttt accagccgca aatcttcgca
     2461 gaaacgggtg aactgtacgg catcgaagcc cttgctcgct ggcacgatcc ccagcatggt
     2521 catgtacccc cttcacggtt tattcctctc gcagaagaga ttggtgaaat cgaaaatatt
     2581 gggcgctggg tcatcgcgga agcttgccgt cagttagcag aatggcgtag ccagaatatt
     2641 catatcccgg cgttatccgt gaacttgtcg gcgctgcact ttcgcagtaa tcagctgcct
     2701 aatcaggtgt ctgatgcaat gcaagcctgg ggtattgacg gccaccagct gacggtagaa
     2761 atcacggaaa gcatgatgat ggaacacgat accgaaatct ttaagcgcat tcagatcctg
     2821 cgcgatatgg gcgtgggctt atcggtagat gattttggca cgggcttttc cggattatcc
     2881 cgcttagtca gtcttccggt aacggaaatc aaaattgaca aaagttttgt cgatcgttgt
     2941 ctgacggaaa aacgcatcct tgccttactt gaagccatta ccagcattgg gcaaagcctc
     3001 aatttaaccg tcgtggcgga aggcgtcgaa accaaagagc aatttgagat gctacgcaag
     3061 atccactgtc gcgttattca gggatatttc ttttcccgcc ccctacccgc cgaggaaatt
     3121 ccaggctgga tgagcagcgt gttaccgctg aaaatctgac aaattcctct cgcccgcact
     3181 cgcgggtttt catttaacgt gacactgtca cttaaatact gtgatttcag ctacgattcc
     3241 gggagattcc ttccttaact tcccctcttg caccaaaact gcactacgct gcacaaatgt
     3301 tgatcaaaag ttaaaaaata taactttaag ccagcattct ggcgcttatc ccggcatggc
     3361 atgagatctg cataagcgga aagcgcagca atttttgtct taatacagga accgtttatg
     3421 tcggatacca ccgaactggt tgatttagcc gtgatcttcc ctgatctgga gatcgaattg
     3481 aaatacgcct gcgctgataa catcacaggt aaagctattt accagcaagc gcgttgtctg
     3541 ttacacaagg atgcgattac cgcgctggcg aaaagtatca gtatcgccca gctgtcaggc
     3601 ttacaactgg tgatttacga tgcgtatcgc ccacaacaag cacaggcgat gttgtggcaa
     3661 gcctgcccag acccgcaata tgttgttgat gtgacggtcg gttctaatca cagccgtggc
     3721 acggcgatcg acctgacgct tcgtgatgag cacgggaaca tcctggatat gggcgcaggg
     3781 ttcgatgaaa tgcacgagcg ctcccatgcc tatcaccctt ccgtcccgcc cgccgctcag
     3841 cgcaatcgcc tgttgctgaa tgcgataatg actggtggcg gttttgtcgg tatctccagc
     3901 gaatggtggc acttcgaatt accccaggca gcgagttacc ctctgctcgc tgatcaattc
     3961 tcttgtttta tatcccctgg cacacagcac gtcagttaat caggaaactg tcatgaagag
     4021 atcgatatcg tttcgtccca cattgctcgc gctcgtcctt gccacaactt tcccggttgc
     4081 gcacgccgcc gtaccgaaag atatgctggt gattggtaag gccgccgatc cacaaaccct
     4141 cgacccggcg gtaacaatag ataataacga ctggacagtg acctacccgt cttatcagcg
     4201 gctggttcag tacaaaacgg acggtgataa aggctcaacc gacgttgaag gcgatctggc
     4261 aagtagctgg aaagcgtctg acgatcaaaa agagtggacg ttcaccctga aggacaatgc
     4321 taaatttgcc gatggcacac ctgtcactgc cgaagcagta aaactctctt ttgagcggtt
     4381 actaaaaatc ggccaggggc cagcagaagc atttcccaaa gatttaaaga ttgatactcc
     4441 cgacgaacat acggtgaagt ttacccttag ccaaccattc gcaccgttcc tctacacgct
     4501 agcgaatgac ggtgcatcca ttatcaatcc ggcggtctta aaggaacatg cagcggatga
     4561 tgctcgcggc ttcctcgcgc aaaataccgc cggttccgga ccatttatgc tgaaaagctg
     4621 gcaaaaaggt cagcaattag ttctggtgcc aaatccgcat taccccggca ataaaccgaa
     4681 ctttaagcga gtatcggtaa aaattatcgg tgaaagtgcc tcccgtcgcc tgcagctctc
     4741 ccgtggcgac attgacattg ccgatgcgct gccggtggat caactcaacg ccctgaagca
     4801 ggaaaataaa gtcaatgtgg cagagtatcc gtcactgcgc gttacctatc tgtatctgaa
     4861 taacagcaaa gcgcctctta atcaggcgga tctgcgtcgg gccatttcct ggtctaccga
     4921 ttatcagggc atggttaacg gcattctgag tggtaacgga aaacagatgc gcggcccgat
     4981 tccggaaggc atgtggggct acgatgcgac ggcaatgcaa tacaaccatg acgaaacgaa
     5041 agccaaagcc gaatgggata aagtgacgag caaacccacc agcctgacgt ttctctactc
     5101 cgataacgat ccgaactggg agcctattgc tctggcgaca caatccagtc tcaacaagct
     5161 gggcatcaat gtgaagctgg aaaagctggc gaacgccacc atgcgcgaca gagtgggtaa
     5221 aggtgattac gacattgcga ttggcaactg gagtccggat tttgccgacc cgtatatgtt
     5281 tatgaattac tggtttgagt cagacaaaaa aggtctgccg ggtaaccgct cgttctatga
     5341 aaacagtgag gtcgataagt tactgcgcaa tgcgcttgcg accaccgacc agacgcagcg
     5401 tacccgggac taccagcagg cacagaaaat cgtcattgat gacgctgctt atgtgtacct
     5461 gttccagaaa aactaccaac tggcgatgaa caaagaggtg aaaggctttg tgttcaatcc
     5521 catgctggaa caggtcttca atatcaatac catgagtaaa taacgtgacc ttctggagta
     5581 ttttacgcca acgctgctgg gggctggtgc tcgtggtggc gggcgtctgc gtgattacgt
     5641 ttattatctc gcatctgatc cctggcgatc cggcgcggtt actggcgggt gaccgcgcca
     5701 gcgatgctat cgtggaaaat attcgccagc aactgggact ggaccagcca ctgtatgtac
     5761 agttttaccg ctacttcagc gacctgtttc atggtgacct gggaacatcc attcgtaccg
     5821 ggcgtccggt gctggaagag ttgcgtatat ttttcccggc gacgctggaa ctggcttttt
     5881 gtgccctgct gctggcactc ctgattggca tcccgctggg catactctct gcagtctggc
     5941 gaaatcgctg gctggatcat ctggtgcgaa taatggccat taccggaatc tccacacctg
     6001 cgttctggct tggactgggc gtcattgtgc tgttttatgg tcatctgcaa attcttcccg
     6061 gcggcggaag gcttgatgac tggctggatc caccaacgca cgttaccggc ttttatctgc
     6121 tcgatgcgct gcttgaaggc aacggtgaag tcttcttcaa tgcgttgcaa catctcatct
     6181 taccggcatt aacgctggcg ttcgttcacc tgggaattgt cgctcgccag atccgctcag
     6241 cgatgctgga acaattgagt gaagactaca ttcgtaccgc ccgggccagc ggcttgcccg
     6301 gctggtatat cgttttatgt tatgcgctac ccaatgcgtt gatcccatcg attaccgtat
     6361 tgggtctggc gctgggcgat ttgttgtatg gcgcagtgct caccgaaacc gtttttgcct
     6421 ggcccggaat gggtgcatgg gtagtaacat caatacaggc gctcgacttc ccggcagtga
     6481 tgggctttgc cgtcgtggtt tcatttgctt atgtgctggt caacctggtg gtggatttgc
     6541 tctatttgtg gattgatccg cgtatcggac gtggaggtgg tgaatgatgc taagcgagga
     6601 aacgtccgcc gtacgcccac aaaaacaaac gcgatttaac ggtgcaaaac tggtatggat
     6661 gctgaaaggc agtccgctca ccgtgaccgg cgcagtcatc attgtattaa tgctattgat
     6721 gatgattttt tcaccgtggc ttgcgacgca tgatcccaac gccattgatt taaccgcccg
     6781 ccttttgccg ccttctgcgg cgcactggtt tggcaccgat gaagtgggac gcgatctgtt
     6841 cagccgcgta ctggtcggca gtcagcaatc aattctcgcc ggattagtcg tggttgccat
     6901 tgcgggtatg attggttcgc tactcggatg tctatccggt gtgcttggcg gacgcgcaga
     6961 cgccatcatc atgcgcatca tggacattat gctgtcgatt ccttcgttgg tactgacaat
     7021 ggcactggct gccgctctcg ggccgagttt atttaacgcc atgctggcga ttgctattgt
     7081 gcgaattccc ttttatgtgc gcctggcgcg ggggcaagca ttagttgtac gccagtatac
     7141 ctatgttcag gcggcgaaaa cctttggtgc gtcgcgttgg catctgatca gctggcatat
     7201 tttacgtaac tccctgccgc cgctgatcgt gcaggcatcg ctggatatcg gtagcgcgat
     7261 tttaatggcc gccacgttgg gatttattgg cctgggtgct caacaaccga gtgctgaatg
     7321 gggcgcgatg gtagctaatg gtcgcaacta tgtactcgat cagtggtggt attgcgcatt
     7381 tccgggggca gcgattttgc ttaccgccgt cgggtttaat ctctttggtg atggtattcg
     7441 cgatctgctt gacccgaaag caggaggaaa gcagtcatga cccaacccgt tctggacatt
     7501 caacagctgc atttgagttt ccccggtttt aacggttatg ttcacgtgct caacaatgtg
     7561 tccttgcaga ttaaccgcgg tgaaattgtc ggtctggtgg gagaatccgg ctcaggtaaa
     7621 tcagtcaccg caatgctgat tatgcgtctg ctaccgacgg gcagttattg cgtacatcgg
     7681 gggcagattt cactgctggg agacgatgtt cttaacgctc gggagaaaca gcttcgtcag
     7741 tggcgcggcg cacgagtggc gatgatcttt caggaaccga tgaccgccct caatccgaca
     7801 cgtcgaatag gtcttcagat gatggacgtg atccgccatc atcaaccaat aagtcgtcgg
     7861 gaagccagag ctaaagcgat taccctgctg gaagagatgc aaatcccgga tgccgtggaa
     7921 gttatgtcgc gctatccgtt tgagctttca ggtggtatgc gccagcgggt aatgattgcg
     7981 ctggcattct cctgcgagcc gcaattgatt attgccgacg aaccgactac ggcgctggac
     8041 gtcacggtac agttgcaggt actgcgtttg cttaaacata aagctcgcgc cagtggaact
     8101 gcggtactgt tcatcagcca tgatatggcc gtggtgtcgc aactgtgcga tagcgtttac
     8161 gtgatgtatg ccggaagcgt aattgaaagc ggcgtgacgg cagacgttat ccatcatccc
     8221 cggcatccgt ataccattgg tttgctgcaa tgcgcaccag aacatggagt accacgccag
     8281 ccattacccg ccattccagg gacggtacca aacctcaccc atttgcctga cggctgcgct
     8341 tttcgcgatc gttgctatgc ggcaggtgca cagtgtgaaa acgtcccggc gctgacagcg
     8401 tgtggtgaca acaaccagcg ctgcgcctgt tggtatccgc agcaggaggt cataagtatc
     8461 tgacacgtta ttaacgttac gcgacgtcca tatcaatttc ccggcccgta aaagctggct
     8521 tggtaaaact acggaacatg ttcatgcgat taatggtatt gatttacaga tccgccgtgg
     8581 tgaaacctta gggatcgtcg gtgagtcagg ctgcggcaaa agcaccctcg cacagctttt
     8641 aatgggtatg ctgcaaccga gccacgggca atacatccgt tcaggatcac aacgcattat
     8701 gcagatggtg tttcaggacc cgctctcttc gcttaatccg cgcttatcgg tgtggcgcat
     8761 catcacagaa ccgctctgga tagctaagcg tagtagtgaa caacagcggc gagcgttggc
     8821 aaaggagttg gctgtgcagg tgggtattcg tccggagtat ctcgaccgcc tgcctcatgc
     8881 gttctccggc gggcagcggc aacgcatcgc cattgccatt gccagagcac tctcttcgca
     8941 gcctgacgtg attgtgcttg atgagccaac ctcggcgctg gatatctccg tgcaggcgca
     9001 gatcctcaat ttactggtaa cgctacagga aaatcacggg ctgacttatg tgctgatttc
     9061 acacaatgtc tcggtgatac gtcatatgag cgatcgggtg gcggtgatgt atctcgggca
     9121 gattgtagaa ctgggcgacg cgcagcaggt gctgacggca cctgcacatc catacacccg
     9181 attattgctg gattccctcc ccgccattga taaaccgctg gaggaagaat gggcattatg
     9241 taaaacggat ctgccgggaa accgcacgtt gccacaaggc tgttttttct acgaacgttg
     9301 ccctctggca acccacggat gtgaagtccg gcaatcatta gcaataaggg aggacggacg
     9361 tgagctccgg tgctggcggg cgctgtagag atgctaacaa cgcatcaggc attcacgcgc
     9421 ctgacgcgtc atccggcaat gctttacgat ttcaactggt aatccagcgt aatttccgct
     9481 ttcagtacct gagaaaccgg gcatcctgct tttgctttct ggatgatgcc gtcaaaggta
     9541 gaggcatcaa tacccggcac cgcaacttca ctcttcagtg cgattttcgt aatcgcaaaa
     9601 tcggcatcca ctttatccag cgacacatcg gcggtggtat caatcgatgt tggcgtgaat
     9661 cccgcttccc ccagcattaa tgaaagcgcc attgagaaac atgcggcatg cgctgcgcca
     9721 atcagttctt cagggttggt tcctttttcg ccttcaaaac gcgtgttaaa tccatacggc
     9781 tgttggttca gcacgccact ctgggtggat actgttccct tcccgcgttt gatatcgcct
     9841 tcccagtgtg cctgaccttt cttatggatt gtcattgttg ctctcctgtg ggcttaaaaa
     9901 ggaaattata gactacgtga gaaacagcac gaggataagc aggatattcc gaataaaata
     9961 cggcgaaaat attacttctg cacttaaatt aaattcccac aatagataac cgggaatata
    10021 tatctcaaga gatattgcat caatatcaat caactcacca caataataat acatatcaat
    10081 atcatggagt taaaatataa aaatcatcaa ccaggactaa tcttaacaac gaagcggcaa
    10141 atatttgcca tgctgaatgt gctttataaa tctgcgatcc gtagcagaca ccataaatac
    10201 acagacacgg agaatcacta tgtttactta ttatcaggca gaaaattcaa cagcagaacc
    10261 cgctctggtt aatgccattg agcaaggcct tcgggcagag caaggtgttg tcactgaaga
    10321 tgacattctc atggagctga ccaaatgggt ggaagcttct gataacgaca tcctcagtga
    10381 tatctaccag caaacgatca attatgtggt cagtggccag caccctacgc tttaaggtgc
    10441 tatgcttgat cggcaaccta atttaggggt ttagcacgtg tttcttcgct acggcgatgt
    10501 tgtccttaaa actagcaaca ggattgagga gttaaaatga aatcgaaccg tcaggcacgt
    10561 catattcttg gactggacca taaaatttct aaccagcgca aaatagttac cgaaggtgac
    10621 aaatccagcg tggtaaataa cccaaccggc agaaaacgcc ccgctgaaaa gtaattcata
    10681 accatcagtc ctcaatgacg attaaacacc attgcctgcg caatggtgtt tttgttttta
    10741 tctgctttat acttggggcc gacgccctgg cggtaaagca aagacgataa aagcgtgcca
    10801 gggatggata ttcaaaaaag agtgagtgac atggaaccaa aaacaaaaaa acagcgttcg
    10861 ctttatatcc cttacgctgg ccctgtactg ctggaatttc cgttgttgaa taaaggcagt
    10921 gccttcagca tggaagaacg ccgtaacttc aacctgctgg ggttactgcc ggaagtggtc
    10981 gaaaccatcg aagaacaagc ggaacgagca tggatccagt atcagggatt caaaaccgaa
    11041 atcgacaaac acatctacct gcgtaacatc caggacacca acgaaaccct cttctaccgt
    11101 ctggtaaaca atcatcttga tgagatgatg cctgttattt ataccccaac cgtcggcgca
    11161 gcctgtgagc gtttttctga gatctaccgc cgttcacgcg gcgtgtttat ctcttaccag
    11221 aaccgccaca atatggacga tattctgcaa aacgtaccga accataatat caaagtgatt
    11281 gtggtgactg acggtgaacg tattctgggg cttggtgacc agggcatcgg cgggatgggc
    11341 attccgatcg gtaaactgtc gctctatacc gcctgtggcg gcatcagccc ggcgtatacc
    11401 cttccggtgg tgctggatgt cggaacgaac aaccaacaac tgcttaacga tccgctgtat
    11461 atgggctggc gtaatccgcg tatcactgac gacgaatact atgaattcgt tgatgaattt
    11521 atccaggctg tgaaacaacg ctggccagac gtgctgttgc agtttgaaga ctttgctcaa
    11581 aaaaatgcga tgccgttact taaccgctat cgcaatgaaa tttgttcttt taacgatgac
    11641 attcagggca ctgcggcggt aacagtcggc acactgatcg cagcaagccg agcggcaggt
    11701 ggtcagttaa gcgagaaaaa aatcgtcttc cttggcgcag gttcagcggg atgcggcatt
    11761 gccgaaatga tcatcgccca gacccagcgc gaaggattaa gcgaggaagc ggcgcggcag
    11821 aaagtcttta tggtcgatct ctttggcctg ctgacggaca agatgccgaa cctgctgcct
    11881 ttccagacca aactggtgca gaaacgcgaa aacctcagtg actgggatac cgacagcgat
    11941 gtgctgtcac tgctggatgt ggtgcgcaat gtaaaaccag atattctgat tggcgtctca
    12001 ggacagaccg ggctgtttac ggaagagatc atccgtgaga tgcataaaca ctgtccgcgt
    12061 ccgatcgtga tgccgctgtc taacccgacg tcacgcgtgg aagccacacc gcaggacatt
    12121 atcgcctgga ccgaaggtaa cgcgctggtc gccacgggca gcccgtttaa tccagtggta
    12181 tggaaagata aaatctaccc tatcgcccag tgtaacaacg cctttatttt cccgggcatc
    12241 ggcctggggg ttattgcttc cggcgcgtca cgtatcaccg atgagatgct gatgtcggca
    12301 agtgaaacgc ttgcacagta ttcgccgctg gtgctgaacg gcgaaggtct ggtactaccg
    12361 gaactaaaag atattcagaa agtctcccgt gcaattgcgt ttgcggttgg caaaatggcg
    12421 cagcagcaag gcgtggcggt gaaaacctct gccgaagctt tgcaacaagc cattgacgat
    12481 aatttctggc aagccgaata ccgcgactac cgccgtacct ccatctaagc ctgcgcccgg
    12541 tagtgaaggc taccgggcaa ttttcctctc cctttttcag atctcatcca tactgggtag
    12601 tggcgaataa atctcatttg cctcacctgc tatgcagaac atcatccgaa aaggaggaac
    12661 tatgaaggct gcagttgtta cgaaggatca tcatgttgac gttacggata aaacactgcg
    12721 ctcactgaaa catggcgaag ccctgctgaa aatggagtgt tgtggtgtat gtcataccga
    12781 tcttcatgtt aagaatggcg attttggtga caaaaccgac gtaattctgg gccatgaagg
    12841 catcggtgtg gtggcagaag tgggtccagg tgtcacctca ttaaaaccag gcgatcgtgc
    12901 cagcgtggcg tggttctacg aaggatgcgg tcattgcgaa tactgtaaca gtggtaacga
    12961 aacgctctgc cgttcagtta aaaatgccgg atacagcgtt gatggcggaa gagtgcatcg
    13021 tggtcgccga ttacgcggta aaagtgccag atggtctgga ctcggcggcg gccagcagca
    13081 ttacctgtgc gggggtcacc acctacaaag ccgttaagct gtcaaaaatt cgtcccgggc
    13141 agtggattgc tatctacggt cttggcggtc tgggtaacct cgccctgcaa tacgcaaaga
    13201 atgtctttaa cgccaaagtg atcgccattg atgtcaatga tgagcagtta aaactggcaa
    13261 ccgaaatggg tgcagattta gcgattaact cacgcaccga agacgccgcc aaaattgtgc
    13321 aggagaaaac cggtggcgct cactctgcgg tggtaacagc agtagctaaa gctgcgttta
    13381 actcggcagt tgatgctgtc cgtgcaggcg gtcgtgttgt ggctgtcggt ctgccgccgg
    13441 agtctatgag cctggatatc ccacgtcttg tgctggatgg cattgaagtg gtcggttcgc
    13501 tggtcggcac gcgccaggat ctaaccgaag ccttccagtt tgccgccgaa ggcaaagtgg
    13561 tgccgaaagt cgccctgcgt ccgttagcgg acatcaacac catctttacc gagatggaag
    13621 aaggcaaaat ccgtggccgt atggtgattg atttccgtca ctaagaggtc tttgctgcga
    13681 ctcccatgtt cgggtcgcag catcgcacac tctccaacat gaaatggcta aatggattat
    13741 tgttgcgtag gaaggaacaa taatgatagt gttcccagag aaggaacgct actgagagta
    13801 aagacactaa tgaaaatggc caatcatccc cgcccggggg acattattca ggaatcactg
    13861 gacgaactta atgtcagcct gcgcgagttt gccagagcaa tggaaattgc gccctcaacg
    13921 gcaagccgat tgctgaccgg aaaagcagct ttgacgccag aaatggcaat taaactttcc
    13981 gtggtgatcg gcagttcgcc gcaaatgtgg ctgaatctgc aaaacgcctg gagtctggca
    14041 gaggcagaaa aaacggtgga tgtgtcgcga ttacgccgtc tggttacgca ataaaaaatg
    14101 gcaccttata aagcgccatt tgaaactgat gacaaacgca acattgcctg atgcgctacg
    14161 cttatcaggc ctacacgttc acttcaatct attgtatttg aagggttttc atgccggata
    14221 aggcattcac gacgcatccg ggatgaacaa agcccacgtt gtctacaata tgaaaatggc
    14281 gccttttaca gcgccatttt cagcttaaag ttcaaaagag attatatccc ttcttcactc
    14341 tcttttttcg cttctgcctt ctcgatttca cgataccagc gcggatggtg tttcttcgcc
    14401 cagcgacggc tcaccttccc ttcgatcatc cctttaatcg accctttcac ccagaatgcc
    14461 atatacatat ggatcaggat ggcgtggatc aggatgatac ccgcagccgc gtggatcagc
    14521 aggctgtagc gaacaacctg catcgggaag tactgcgcaa agtacggacg ccagataatc
    14581 accccggtca ccagcagcac gaaaatcatg ctcatgatcg accagaacat catcttttgc
    14641 ccggcgttgt acttaccaac atccgccact ttgtgctcat tgccttttaa tacttcgaca
    14701 atgttcaaca gccacggaat atctttctta tccgggatgt tgtgatgcac aaaacgcaca
    14761 aacataaaca tcagtgcgac gaaaatcgca atgccgaaga acgggtgcaa aatgcgtccc
    14821 atctgcggcg taccgaaggt ttgcgtcagc cattgcagcg tcgggaagaa gaacgaaatc
    14881 ccggacagcg ccaccaggaa gaagcaaatc accacggtcc agtgacaggc gcgatcaata
    14941 aatttggtgc gcacaatcat tttcgactta ctcatgatga tcctcctcgt catcgtccac
    15001 ttccttattc gggccaatac cgatgtagtg gaaaatcaac ccggcaaaag tggcaataaa
    15061 gccagccgct gccagcggtt tcaacgcgcc tttccacagg cttaccgagg tgtcgatctt
    15121 cggatctttc ggcagaccgt aatacagctc cggctgatcg gcgtgatgca gcacgtacat
    15181 aacgtgcgta ccaccgaccc ctttcgggtt gtagacgcca gcatgttcgt aaccacgcgc
    15241 tttcagtttc gccacgcgct cttccgccag ctccagcatc tccttcttgg tgccgaagtg
    15301 gatagccccg gtcggacagg ttttcacaca agccggttcc tggccgacgc tgacgcgatc
    15361 gacgcagagc gtgcatttat atacccggtt atcctctttg ttgaggcgcg gaatattaaa
    15421 cggacacccg gcaatgcagt aaccacagcc gatgcagttt tccgactgga aatcgacaat
    15481 cccgttagcg tactgaatga ttgcaccagc agacgggcac gccttcaggc agccgggatc
    15541 ttcacagtgc atacagccgt ctttacggat cagccactcc agcttgccgt tctgttcggt
    15601 ttcgctaaag cgcatcaccg tccaggactt ggcgctcaga tcggcggggt tatcgtaaac
    15661 cccgacgcag tgccccactt catcacggat gtcgttccac tccgaacacg ccacctgaca
    15721 ggctttacag ccgatacagg tggaaacgtc gataagtttt gcgacttctg ctttgtaatc
    15781 acgcacctga gaaggcggcg tgatggagtt agttgcggac cttttgataa tatcctgcgt
    15841 ttccatagcc atctgttcgc ccccttacgc cttctcgatg ttgactaaga acgctttata
    15901 ttccggcgtt tgcgagtttg catcaccgac attcggcgtc agagtgttag cgatataacc
    15961 tttacgcgcg acaccctcaa agccccagtg gattggaata cccaccgttt caacctgctg
    16021 accgtttaca ttcagcggtt tcagacggcg cgttaccacc gccaccgcgc ggataaagcc
    16081 acgcttgctt gagacagtga cacgatcgcc attattaatg cctttcgccg ccgccagcgt
    16141 ttcgctgatt tccacaaact gttccggctg agcaattgcg ttgagcaatg cgtgcttggt
    16201 ccaggtgtgg aagtgctcgg tcagacgata ggtcgtaccc acatacggga actgctcttt
    16261 tttacccatc cgcagcgcgt cttgttcata cagacgaaca accgggttag acaccacgtt
    16321 cggatgcagc gggttagtgc ccagcggcgt ttcaatcggc tcgtagtgtt ccgggaacgg
    16381 accttccgcc attttgttga tggcaaacag gcgtcccatc ccttccggct gcatgataaa
    16441 cggcccggtt ggcgtacccg gtgcggcatt gccgaagtcc ggaatatcgt tacccgtcca
    16501 cttgctgccg ttccactgga tcagcatccg tttcggatcc cacggtttac cattgatatc
    16561 cgccgaagca cggttgtaga gcacgcgacg gttgagcggc cacgcccagg cccatcccag
    16621 cgtattcccc agaccggatg ggtcggagtt atcgcggtta gccatctggt tgccctgctc
    16681 tgtccagcta ccggtgtaga tccagcaaga agatgcggtt gtaccgtcat cacgcagatg
    16741 cgcaaagcta ctcagcaact gacctttctt cgcaatcagc acgccattag cgtcatagag
    16801 atcttccagc gcatagccgt tattctcttt cgccacttcg tcagattgcg gttcgtgcgg
    16861 ctgcttgtag ttccagctca tcttcatcag cggttctacg cctttaccac cttcggcctg
    16921 gtacagctcg cgcagatgat ggtagatacc cgccagaatt tcgccgtcgt tacgcgcttc
    16981 gcccggcgcg tcctgacctt tccagtgcca ctgcaaccag cgaccggagt tagcgataga
    17041 accatcttct tcggcaaagc aggtcgaagg cagacggaat acttcagtct gaatagacgc
    17101 cggatcgaca tcgttcgact cgccgtggtt ctgccagaag gtagaggttt cagtcaccag
    17161 cggatcgata accaccatgt acttcagctt gctcaggcag ctcaccactt tgtttttgtc
    17221 cgggaaggac gcaaccgggt taaagccctg gcagaagtaa ccggtgactt tgccttcgtc
    17281 catcatgttg aaatacttga tgacgtcgta ggtctggtcc cacttcggca gccagtcata
    17341 gccccagttg ttctctttct gcgcggcatc gccatagaaa gatttcatca ggctaacgaa
    17401 gaacttcgga tagttgctcc agtagttcac ctgatcagcc agcgtcgctt tcggcgtgtt
    17461 cgcttccaga tacgactgca aatcaacctg tttttctgac ggaagcgtca gataacctgg
    17521 caggctggta gagagcagac ctaagtcagt cagcccctga atgttggagt gaccacgcaa
    17581 tgcgttcacg ccgccaccgg ccatacccat gttaccgagc agcaactgga tcatcgccat
    17641 agtacggatg ttctgcgcac ccacggtgtg ctgcgtccag cccagcgcgt acaggaaggt
    17701 ggttgtgcga tccggtgcgc tggtggaggc cagcacttca cacactttca ggaagtcggc
    17761 ttttggcgta ccgcagatgt tttcaacgac gtccggcgtg tagcgggaaa cgtgcgcttt
    17821 cagcaggttc cacacacagc gcggatgagt cagtgtttca tcgcgttttg catagccgtt
    17881 ttcatcgaac tgatagttcc aggacgactt atcatactgg cgtttttcag cgtcgtagcc
    17941 gctgaacagg ccgtcttcga aagcaaaatc atcacgcacc agcaggctgg cgttggtgta
    18001 atgtttaacg tattcggcgt tgattttgtt gttttcgatc aggtagcgca aaacgccaga
    18061 caggaacgta atgtccgtac cggaacgaat aggtgcgtaa atatccgcca cagaagcggt
    18121 acgcgtaaaa cgaggatcga caacgatcaa ggttgcatcg ttgttgtttt tcgcttccat
    18181 cgcccagcgg aaaccgacgg gatgcgcttc agcagcgtta ccgcccatta ccattacgac
    18241 gttagcgttt ttgatatcca cccagtggtt ggtcatcgca ccgcgaccaa atgttggagc
    18301 aagacttgct accgttggtc cgtgtcagac gcgcgcctgg ttgtctaccg ccagcatccc
    18361 gagggagcgg gcaaatttct gggtcagcat cccggtttcg ttgctggcac cggaggcaca
    18421 cagcataccg gtagaaagcc aacggtttac cgttacgccc tgctcgttct tttcaataaa
    18481 gttagcgtca cggtcagctt tcatcagctt cgcaatacgg gagaatgctt cttcccagct
    18541 aatgcgctgc catttgtcag aacctggcgc acgatattcc gggtagcgca gacggttttc
    18601 gctgttgacg taatccagca aaccggcccc ttttgggcac agcgcaccac ggcttaccgg
    18661 atgatccggg tcaccttcaa tgtgataaat cgcttctctg gcgtttttcg cgccatcacc
    18721 caggctatac atcaatagcc cgcaacctac ggaacagtat gtgcaggtgt tacggatctc
    18781 tttagcgcgt aataatttgt agtttcgcgc ctgagccagt gcttgcttcg gggcaaagcc
    18841 caatgccgct accgttgttc cagccatacc gcccgcgcag attttaaaaa attgtctgcg
    18901 actgacgtcc attgctttcc tcttttttca ggggtattac tgcgcgagga aactaacagt
    18961 aaaattctga atctttttgc gcgaaatcaa aaagaacacg tgtatgccac aaaataatcc
    19021 gcctgcgcaa attaacctac ctcaatagcg gtagaaaaac gcaccactgc ctgacaggcc
    19081 agttaaaaaa atgctataaa attcagctta atttttaacg gcaagagaga caaaacagcg
    19141 agcatgacac gacaaaaagc aacgctcata gggctaatag cgatcgtcct gtggagcacg
    19201 atggtaggat tgattcgcgg tgtcagcgag gggctcggtc cggtcggcgg cgcagctgct
    19261 atctattcat taagcgggct gctgttaatc ttcacggttg gatttccgcg tattcggcag
    19321 attccgaaag gctatttact cgccgggagt ctgttattcg tcagctatga aatctgtctg
    19381 gcgctttcct tagggtatgc ggcgacccgt catcaggcga ttgaagtggg tatggtgaac
    19441 tatctgtggc ccagcctgac aattctcttt gccattctgt ttaatggtca gaaaaccaac
    19501 tggctgattg taccaggatt attattagcc ctggtcggcg tctgctgggt gttaggcggt
    19561 gagaatgggt tacattatga tgaaatcatc aataatatca ccaccagccc attgagttat
    19621 ttcctggcgt ttattggtgc gtttatctgg gcagcctatt gcacagtaac gaataaatac
    19681 gcacgcggat ttaacggaat taccgttttt gtcctgctaa cgggagcatg tctgtggatt
    19741 tactattttc tcacgccaca accagaaatg gtatttagca cgcccgtcat gattaaactc
    19801 atctctgcgg catttacctt aggatttgct tatgctgcat ggaatgtcgg tatattgcat
    19861 ggcaatgtca ccataatggc ggtaggctcg tattttacgc ctgtactttc ctcagcgctt
    19921 gcagccgtgc tgctcagcgc cccgctgtcg ttctcgttct ggcaaggcgc gctaatggtc
    19981 tgcggcggtt ccctgctctg ctggctggcg acacgtcgtg gttaaaataa ttgccgggtc
    20041 tacggcccgg cagttaatca gataaattcc gacaatatta aaaaataaat t
//
LOCUS       DAZRUF010000055        19745 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZRUF010000055 DAZRUF010000000
VERSION     DAZRUF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.055"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            69..740
                     /locus_tag="ACHXOL_001599"
     CDS             69..740
                     /locus_tag="ACHXOL_001599"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR6808607.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            750..1946
                     /locus_tag="ACHXOL_001600"
     CDS             750..1946
                     /locus_tag="ACHXOL_001600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR6808608.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIKRGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            1946..2506
                     /locus_tag="ACHXOL_001601"
     CDS             1946..2506
                     /locus_tag="ACHXOL_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR6808609.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2508..3281
                     /locus_tag="ACHXOL_001602"
     CDS             2508..3281
                     /locus_tag="ACHXOL_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR6808610.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3469..3744
                     /gene="frmR"
                     /locus_tag="ACHXOL_001603"
     CDS             3469..3744
                     /gene="frmR"
                     /locus_tag="ACHXOL_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR6808611.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3779..4888
                     /gene="frmA"
                     /locus_tag="ACHXOL_001604"
     CDS             3779..4888
                     /gene="frmA"
                     /locus_tag="ACHXOL_001604"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR6808612.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            4981..5814
                     /gene="fghA"
                     /locus_tag="ACHXOL_001605"
     CDS             4981..5814
                     /gene="fghA"
                     /locus_tag="ACHXOL_001605"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR6808613.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6040..6579)
                     /locus_tag="ACHXOL_001606"
     CDS             complement(6040..6579)
                     /locus_tag="ACHXOL_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR6808614.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6681..7892)
                     /gene="mhpT"
                     /locus_tag="ACHXOL_001607"
     CDS             complement(6681..7892)
                     /gene="mhpT"
                     /locus_tag="ACHXOL_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR6808615.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8179..9192)
                     /gene="mhpE"
                     /locus_tag="ACHXOL_001608"
     CDS             complement(8179..9192)
                     /gene="mhpE"
                     /locus_tag="ACHXOL_001608"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR6808616.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9189..10139)
                     /gene="mhpF"
                     /locus_tag="ACHXOL_001609"
     CDS             complement(9189..10139)
                     /gene="mhpF"
                     /locus_tag="ACHXOL_001609"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR6808617.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10136..10945)
                     /gene="mhpD"
                     /locus_tag="ACHXOL_001610"
     CDS             complement(10136..10945)
                     /gene="mhpD"
                     /locus_tag="ACHXOL_001610"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR6808618.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10955..11821)
                     /gene="mhpC"
                     /locus_tag="ACHXOL_001611"
     CDS             complement(10955..11821)
                     /gene="mhpC"
                     /locus_tag="ACHXOL_001611"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR6808619.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11839..12783)
                     /gene="mhpB"
                     /locus_tag="ACHXOL_001612"
     CDS             complement(11839..12783)
                     /gene="mhpB"
                     /locus_tag="ACHXOL_001612"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR6808620.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12785..14449)
                     /locus_tag="ACHXOL_001613"
     CDS             complement(12785..14449)
                     /locus_tag="ACHXOL_001613"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR6808621.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLHVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14491..15324
                     /locus_tag="ACHXOL_001614"
                     /pseudo
     CDS             14491..15324
                     /locus_tag="ACHXOL_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            15401..16483
                     /gene="lacI"
                     /locus_tag="ACHXOL_001615"
     CDS             15401..16483
                     /gene="lacI"
                     /locus_tag="ACHXOL_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR6808622.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16606..19680
                     /gene="lacZ"
                     /locus_tag="ACHXOL_001616"
     CDS             16606..19680
                     /gene="lacZ"
                     /locus_tag="ACHXOL_001616"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR6808623.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
BASE COUNT         4638 a         5432 c         5161 g         4514 t
ORIGIN      
        1 gccagagatt acgtctctga gtgcatcgtt gtgcttgtag tataataaat aaaatggagt
       61 gtcattaagt ggataaggtt ttagattcag ccctcctttc ttcggcaaat aaaagaaagg
      121 gtatccttgc tattggcgca catcctgacg atatagaatt aggctgtggc gcatcgcttg
      181 ctcgtcttgc gcaaaaagga atttatatcg cagcagtggt gatgactacc ggcaactctg
      241 gtacagatgg aataatcgat cgccatgaag aatcgcgcaa cgccctaaag atattagggt
      301 gccaccaaac tattcatctt aattttgctg acacccgcgc tcatttacag ctcaatgata
      361 tgatttccgc cctggaagac atcattaaaa atcaaattcc ttctgatgtt gaaatcatgc
      421 gggtatatac catgcatgat gccgaccgcc atcaggatca tctcgctgtt tatcaagctt
      481 caatggttgc ctgccgcact attccacaaa ttctcggcta cgaaacccca agtacctggc
      541 tttcatttat gcctcaggtt tttgaatccg ttaaagaaga atatttcaca gtcaaacttg
      601 ctgcattaaa aaaacataaa agccaggaac gacgtgatta tatgcgtcat gatcgcctgc
      661 gtgcagttgc acaatttcgc gggcaacagg tcaatagcga tctgggtgaa ggctttgtaa
      721 ttcataaaat gattctttga gcaaattgca tgaaaacctg gatatttatc tgtatgtcca
      781 tagcaatgtt gctatggttt ttaagtacgc taagacgtaa acccagtcaa aagaaaggct
      841 gtattgacgc cattatacct gcgtataacg aaggcccgtg tctggcgcag tcactggata
      901 atctactgcg taacccttat ttttgccggg taatttgcgt taacgacggc tccacggaca
      961 ataccgaagc ggtcatggcg gaagtcaaac gcaaatgggg cgaccgcttt gttgccgtca
     1021 cgcaaaaaaa taccggtaaa ggtggtgcgc tgatgaatgg cctcaattat gccacctgcg
     1081 accaggtttt tttaagtgat gccgacacct atgttccgcc cgatcaagac ggaatgggct
     1141 atatgctggc agaaattaag cgcggtgctg atgccgtagg cggcattccc tctactgcgt
     1201 tgaaaggcgc ggggctgtta ccgcacatcc gcgcgaccgt aaagttgccg atgattgtta
     1261 tgaagcgcac gctacagcag ctcctgggcg gcgcaccgtt tattatcagc ggtgcctgcg
     1321 gaatgttccg tactgatgta ttgcgtaagt tcggtttctc ggatcgtact aaagtcgaag
     1381 accttgatct cacctggaca ttagtggcaa acggctaccg tattcggcag gcgaatcgct
     1441 gcatcgtata cccacaggaa tgcaacagtc cgcgtgagga gtggcgtcgc tggcggcgtt
     1501 ggattgtggg ctacgcggtc tgtatgcgcc tgcataaaag gcttttattt agccgcttcg
     1561 gtatcttcag tatatttcct atgctgttgg ttgtgattta tggcgttggg atttatctca
     1621 ctacctggtt taatgaattc atcaccaccg ggccgcatgg tgtggtgttg gcaatgtttc
     1681 cgcttatctg gatcggcgta gtttgtgtta ttggtgcttt tagcgcctgg tttcatcgtt
     1741 gctggttgtt ggtgccttta gcgccgcttt ccgttgtgta tgtattatta gcttatgcca
     1801 tctggattat ctatggactt attgcctttt ttactggacg cgaacctcag cgcgacaaac
     1861 ccacccgcta ttccgcactg gtggaagcgt caaccgctta ttcccaacct tctgtcacag
     1921 gaactgaaaa actatctgaa gcttaatgtt aaagagaaaa attattcaga ttgcagacca
     1981 ggtaattatt gatgaaactg caggtgaagt tgttatcggc gcgaataccc gtatttgtca
     2041 tggtgccgtt attcagggtc cggtagtgat tggcgcaaac tgcctgatag gtaattatgc
     2101 gtttattcgt cctggcacaa taatcagcaa tggtgtaaaa attggttttg ccaccgaaat
     2161 taaaaatgcg gttattgaag cggaagcaac gattggtccg caatgttata ttgccgactc
     2221 ggtagttgca aaccaggcat atttgggcgc acaagtacgt accagtaatc atcgtctgga
     2281 tgaacagcct gtgtctgttc gaactccaga gggaattatc gctaccggat gcgataaatt
     2341 aggttgttat atcgggcagc gttcacgcct tggcgtacaa gttattattt tgcctgggcg
     2401 aattatttcc ccgaacacgc aacttggccc gcgcgtgatt gtagaacgta atttacctac
     2461 cggaacttat tcactccgac aagaacttat ccgtacagga gattaaaatg ataaaacgga
     2521 cgttattagc ggcggccatt tttagcgcat tgcccgctta tgccgggtta acttccatta
     2581 ccgcgggcta cgattttacc gattattctg gcgatcatgg caaccgtaat ttagcgtatg
     2641 ctgaactggt ggcgaaagtt gaaaacgcaa cgctgctttt taatctttcc caggggcgtc
     2701 gtgattatga aactgaacat ttcaatgcca ctcgcggtca gggtgcggtc tggtataaat
     2761 ggaataactg gctgacaacc cgaacgggta ttgcctttgc ggataatacg ccggtctttg
     2821 cccgccagga ttttcgtcag gatattaacc tggccctgtt gccaaaaacg cttttcacga
     2881 ccggttatcg ctacactaaa tattacgatg atgtcgaagt cgatgcctgg caaggcggcg
     2941 tatcactcta tactggcccg gtgatcacca gctaccgcta tacccattat gactccagcg
     3001 atgcaggtgg tagttatagc aatatgattt ccgtgcgtct gaatgacccg cgcggcactg
     3061 gttatacgca actatggcta agccgcggaa caggcgctta cacctatgac tggacgccag
     3121 aaacacgcta cggcagcatg aagagcgtca gtttgcaacg tattcaaccg ctgactgagc
     3181 aacttaatct cgggctgacg gcaggtaaag tgtggtacga caccccaacc gatgattata
     3241 acggtctgca acttgcagcc catctgacct ggaaattctg attgcttctg ccgcccgcta
     3301 tccggggcgg ccttccctgc cgattagccc cactcccttt cctcgttgtt ttccgaccac
     3361 attcaccgga taaattttat tctccagcgt tatatactat agggaggtat gcattgacat
     3421 atagcatacc tccctatagt atattgcgtg cagatgatga ggtgcgaaat gcccagtact
     3481 ccggaagaga agaaaaaggt ccttactcga gttcgtcgta ttcgggggca gattgatgct
     3541 ctggaacggt cgctggaggg tgatgccgaa tgccgtgcca tactccaaca gatcgctgcc
     3601 gttcggggcg cggctaacgg gctgatggca gaagtgcttg aaagccatat ccgggaaacg
     3661 tttgaccgaa atgactgcta cagccgcgaa gtcagccaat ccgttgacga cactattgaa
     3721 ctggttcgag cctatcttaa atagctgaat ctattaccat attgaggaag agcgagagat
     3781 gaaatcacgt gctgccgttg catttgctcc cggtaaaccg ctggaaatcg ttgaaattga
     3841 cgttgcacca ccgaaaaaag gtgaagtgct gattaaagtc acccataccg gcgtttgcca
     3901 taccgacgca tttaccctct ccggggatga cccggaaggt gtattcccgg tggttctcgg
     3961 tcacgaaggg gccggcgttg tggttgaagt cggtgaaggc gtaaccagcg tcaaacctgg
     4021 cgaccatgtg atcccgcttt acaccgcgga gtgcggcgag tgtgagttct gtcgttctgg
     4081 caaaactaac ctctgtgttg cggttcgcga aacccagggt aaaggcttga tgccagacgg
     4141 caccacccgt ttttcttaca acgggcagcc gctttatcac tacatggggt gctctacatt
     4201 cagtgaatac accgtggtcg cggaagtgtc tctggccaaa attaatccag aagcaaacca
     4261 tgaacacgtc tgcctgctgg gctgtggcgt gaccaccggt attggcgcgg tgcacaacac
     4321 agctaaagtc cagccaggtg attctgttgc cgtgtttggt cttggcgcga ttggtctggc
     4381 agtggttcag ggcgcgcgtc aggcgaaagc gggacggatt atcgctatcg ataccaaccc
     4441 gaagaaattc gatctggcgc gtcgcttcgg tgctaccgac tgcattaacc cgaatgacta
     4501 cgacaaaccg attaaagatg tcctgctgga tatcaacaaa tggggtatcg accatacctt
     4561 tgaatgcatc ggtaacgtca acgtgatgcg tgcggcgctg gaaagtgcgc accgcggctg
     4621 gggtcagtcg gtgatcatcg gggtggcagg ttctggtcag gaaatctcca cccgtccatt
     4681 ccagttggtc actggtcgcg tatggaaagg ttccgcgttt ggcggcgtga aaggtcgttc
     4741 ccagttaccg ggtatggttg aagatgcgat gaaaggtgat atcgatctgg aaccgtttgt
     4801 cacgcatacc atgagccttg atgaaattaa tgacgccttc gacctgatgc atgaaggcaa
     4861 atccattcga accgtaattc gttactgatt tcccgcaggt ataccccgtc cacttcagac
     4921 ggggttctta atactctccc cgggcagccg tccgggggat taaccgtgag ataatgactg
     4981 atggaactca ttgaaaaaca tgccagcttt ggcggctggc aaaatgtgta tcggcattat
     5041 tcccaatcac tgaaatgtga aatgaatgtc ggcgtctatc tcccaccaaa agccgcgaat
     5101 gaaaaattgc cggtgctgta ctggctttca ggcctgacct gcaacgagca gaatttcatt
     5161 actaaatcgg ggatgcagcg ttacgcggct gagcacaaca ttattgttgt tgcgccggac
     5221 accagtccgc gaggcagtca tgtcgcagat gccgaccgtt acgatctcgg gcaaggtgcc
     5281 gggttttacc tgaacgcaac gcaagcgccg tggaatgaac attacaaaat gtatgactat
     5341 atccgcaacg agctgccgga tttagtgatg caacattttc cggcaacgac cagaaagtct
     5401 atctctggtc attctatggg cgggctgggc gcgctggtgc tggcgttacg taacccagat
     5461 gaatatgtca gcgtctcggc gttttcgccc attgtctccc catcgcaagt accgtgggga
     5521 cagcaagcct ttgccgcata tcttggtgaa aataaagatg cctggttgga ttacgatccg
     5581 gtgagtctta tttcacaagg tcaacgcgtt gcggaaatca tggttgatca ggggttgagt
     5641 gatgattttt acgcggaaca gctgcggact ccaaatcttg aaaagatctg ccaggagatg
     5701 aatatcaaga cgttaatccg ttatcacgag ggttacgacc acagctatta ttttgtctcc
     5761 agttttattg gcgagcatat tgcctaccac gccaataaac tgaatatgcg ttgataatag
     5821 tgcacgactg ccggatgcgg cgtgaacgcc ttatccggcc tacacttcgc ccgtaaaccg
     5881 taggcctgat aagacgcgca aagcgtcgca tcaggcatgg cgcacgactg ccggatgcgg
     5941 cgtgaacgcc ttatccggtc tacacttcgc ccataaaccg taggcctgat aagacgcgca
     6001 tagcgtcgca tcaggcatct gcgcacgact tatctatgtt taccacatca aatcatcagg
     6061 cactttgaag tcggcatacg gatcgtcttc atcctgctct tcggcactga gcgcgctgtg
     6121 caacacaata ctgctggcat cacgctgagc aattttatcg gcaacactgg cgggaataat
     6181 ggtgtattca ccttcgctat tgttatcgac caacaagcgg gcaatcgcca gacgaccatt
     6241 aatcaactgc gcctgtgtga gcttatcgac aaaaatcttc ttaatcagat tgccgtcagt
     6301 gaagttaaaa ccaatatcgc cattggcaat ggtgattcgg ttcatttcaa tgagctgctt
     6361 cacctgggct ttatattctt tcgccaacgc cgcttgtttt tgctgttcgc taagctgttt
     6421 atcacgctca agctgtgcct ttttattttc ttctaccgca gcccgagctt cacgcgcctg
     6481 aacgcgcgat tttttcgccg ttctctcgac cttcgccgct tttttgctgg tgactaatcc
     6541 tgctttgagc aactgctctt gtaaggtaag ttttgccatt tttgtttcca aacccgctga
     6601 ataattttta gcgagtatac ctgtaaacgc cgtgggtgta ttgggggcaa gtccctttcg
     6661 cccctgctta ataaggcgcg tcaggcatcg gcacacggct gcattcttga tctccgactc
     6721 atcaaaataa acaccgccaa cccagcaaca agaatacccg gtgcagaagc ggccattacg
     6781 ccgaccgtgc cagtgcctaa tgccagcatt ttcccggcca gtaacggacc gctcatagcc
     6841 cccagacgcc ctaccgccac ggctgttccc acacctgttg cgcggatctg cgaactgtaa
     6901 aacaacggtg ccagggcata caaaacgctt tgcccacctg tcgcaaacaa ccccgcgaca
     6961 aatcccgcca gcaacatacc gttaaatgac gacaccgttc caagcgccag cagcgaagct
     7021 aacatgccgc tataaatcag tagcgacatg gttactggac gcagcttatc catcaatgcg
     7081 cccaacatta acgtcccgct tgccgccccc atttgcaggg caaacatcac ccctgccgcc
     7141 tgcgatggct ggaatccttg ctccaccaaa agtagcggta gccagttgat caacatataa
     7201 accaccagca gagtgaagaa ataacacaac cacagcagca gcgtcgcggt tgccgtttct
     7261 ggcgcaaata aggcacgcag tggtggcgca gcctgttttt cgccagcgaa aaccgctgac
     7321 tccggcagcc agcgcattaa tagcggcacc agaatcaacg gcaccacacc acctacccaa
     7381 aacaccgttt gccatgctaa gtttgccccc gcgaaaccca gtgtcgccgc cagcgccgcg
     7441 ccaatgggaa caccgcaata catcaggctc actgccgtcc cacgaaaacg tggacccgcg
     7501 gcttcagatg tcagggcgat aagattcggc aacgccgccc ccagcccgac accggtcatc
     7561 agccgcgcaa agaccagtga ggggaaatcc caggcaatcg ccgttgccag tgagaacaaa
     7621 ccaaacagcg caactgagcc aatcaaaata cgcttacgac cataacggtc tgccagcatc
     7681 ccgccaacca acgcgccggg tagcaaaccg agtattccgg cgctaaatat ccagcccatt
     7741 tgcattttat cgagtgcgaa agcctgggcg atgccacccg ccgcaatgcc agccgcctga
     7801 agatccagcc cttccatcag agcgaccaaa aaacaaagcc cgatggtcag catcaggcgg
     7861 gatgaagatg atgaaggggt acgagtcgac ataagatgaa cctcattgcg agtgcaggtg
     7921 aatcgcgact gatacattgc cagccgcgta atcagaacta tttaatgact gcctacgggc
     7981 gaaatgtagc gaaatgtagg ccggataagg cgttcacgcc gcatccggca gtcgtgcgca
     8041 gatgcctgat gcgacgctaa cgcgtcttat cagacctaca aattctcacg ccatccgtag
     8101 gccggacaag gcgttcacgc cgcatccggc tgtcgtgctg ggatgggcat acaaaatgca
     8161 cgttatcggc agtcatcatt atttgttgtt gcgcagatcc agcgccacgt cgacgatcat
     8221 atcctcctgg ccgccaacca tccggcgttt gcccagctca acgagaatat ccaccgcact
     8281 taagccataa cgcgccgccg ccgtttcaca gtgacgcagg aagctcgagt aaacaccagc
     8341 gtatcccagc gccagcgttt cgcgatcgac tcgtaccgga cgatcctgca acggacgcac
     8401 caggtcgtcg gcggcatcca ttaacgcata gagatcggtc ccatgctgcc agcccagttt
     8461 atccgcggcg gcaataaaca cttccagcgg tgcgttacct gcgcccgcgc ccattcccgc
     8521 gaggctggcg tcgattcggt cgcagccctc ttccaccgcc gcgatagagt tcgccacgcc
     8581 aagactcagg ttatggtgag cgtgcatgcc ggtttgcgtt tctggtttca acactgcttt
     8641 cagggcgcgg aaacggtcac ggatatcgct catgttcatc gcaccgccag aatccaccac
     8701 ataaatacag gtcgcaccgt agccttccat cagctttgcc tgcttggcga gattctccgg
     8761 cgtggtcata tggctcatca tcagaaaacc aacggtgtcc attccgagct cgcgggcata
     8821 ctgaatatgc tgggcggaaa catcagcttc ggtacagtgc gttgccacac gaaccacccg
     8881 cgcgccagcc tgccaggcat ttttcagatc gtgaatagtg ccgattcctg gcagcaacaa
     8941 cgtcgcgatt ttggcgtgcc tcaccacatc cgccgccgct tcaatccatt caaggtcgct
     9001 atgtgcgccg aaaccatagt taaagctgga accttgcaaa ccgtcgccgt gggccacttc
     9061 aatcgaatcc acgcgggcat cgtccagtgc tttggcaatc tggcgaacgt tttccagcga
     9121 atactgatga cgaatggcgt gcataccgtc acgcaatgtg acgtccgaga tataaagttt
     9181 tttaccgttc atgccgcttc tcctgccttg cgcgccagtg actgggccat tttttccgct
     9241 gtcgccagcg cactggaagt cataatatcg aggttgcccg cataggcagg cagataatgc
     9301 gctgcgcctt cgacttccag ccagaccgct gttttcagtc cggagaattg ccccacgccc
     9361 ggtaaattga ccggtttatc ctgcgggata acttcaaact gcacgcgctg tttcaggcga
     9421 taacccggta cgtaagcctg caccgcctca gccatttcat tgattgaggc ttcgatatca
     9481 tcttgtgaag cttcgtcgct caatacatac accgtgtcac gcatcatcaa cggtggctct
     9541 gctgggttaa gcacaataat cgccttccct tttgccgcgc cgcccaccac ttcaatggct
     9601 cgggaagtgg tttccgtaaa ttcatcgata ttggcacgcg tgccaggtcc ggcagattta
     9661 ctggcgatag aagcgataat ttcggcgtaa tgaacacgcg ccacgcgtga aactgccgcc
     9721 accattggaa tggtggcctg gccgccgcag gtgaccatgt tgacgttcag ttgatcgacg
     9781 ttcgcctcga ggttaaccac cggcacgcag taagggccga tggcagcagg cgtcaggtca
     9841 attaagcgaa tatccggttt cgcttcgcgt aaagcggcat cgtttttcac atgcgcgccc
     9901 gcgctggtcg catcaaatac aatgtcgata tcagcaaatt caggcatgtt catcagcccg
     9961 atcacccctt catgggtggt ggcgacgccc atacgtctgg cacgcgccag accgtcggac
    10021 tgaggatcaa tgccgaccat caccgccatc tccagatgct gaccgtgacg caaaatttta
    10081 atcatcagat cggtgccaat gttgccagaa ccgataatgg cgactttacg cttactcatg
    10141 acagacttcc ttttggggct gcgcttgaaa atgtcgcagc aactgaacct atgccttcaa
    10201 tatgggcttc aaaacgatcg cccgcattca ccgccaccat cgggcctaat gccccggtaa
    10261 gaatgatatc tccggcgcgc agcggttcac ccagactggc cattttgcgt gccagccaga
    10321 cggcggcatt aagcggatgt cccaggcatt cgctgccgcg cccgctggaa acctcttcgt
    10381 tattacgcgt catcttcatg gcgcagtttt tcaggtctaa ccccgccgga cgttgcgccg
    10441 gaccgccgat gacatacacc ccgcaggaag cgttatctgc cacggtatcg acaaactgaa
    10501 tcgaccagtc gcgaatgcgg ctccccacca cttccagcgc cggaagtacc cattcaatgg
    10561 cgttatacag ttcgtcgaag gtgatatcag ttgcgggtaa atcgcggttc aacaccagtg
    10621 cgatctccgc ttcaatgcgg ggttgcagaa cacgggaaaa aggaatgatt tcgttatcgc
    10681 cataacacat gtcggcaaat aacgtcccaa aatccggttg atcaacgccc agttgttgtt
    10741 gcactttcgg atgcgtcagg cccactttac gccctaccac gcgacgcccc tgcacaacgt
    10801 catattgcac atttatgtgc tgaatggcgt aggccgcttc agcgtgatcg ataccaatca
    10861 gatcgcgcag cggcgcaatc gcttcgccct gctctgcggc gcggcgtaaa tccgccgcca
    10921 gttgctcaag agtatgcttc gtcatgacca ttccttaagg gcgtgcgagg aaattcagca
    10981 ccaattgatt gaaagcgtcg gcatgttccc actgcgccca gtgaccacag tcgcggaaga
    11041 tatgcagttc agaaccggca atgccggaca gcagacgcag acccgcatcc atcggcacaa
    11101 agcggtcgtt gcgcccccag acaatcaggg tttgcgcttt gatttccgcc agacgtgggc
    11161 caaaatccgg gaactgtttc ggattagctt ccaggctctt aacgaagttt tccaggtgat
    11221 cgcggcgcga cagcatatta ttcaggcgcg cttcaaacag ggcgtcggtc aaatcgctgg
    11281 tatcgaaaac gaagatatcc atcatcagct tcaggttttc gatagtcggc tgacgataaa
    11341 gctgattcag tcgcttaata ccttcggttg gcatcggcgt aaacaaactc atgccgcccg
    11401 taccgccgcc catcagcacc agtttgccga cgcgctccgg ccagttaagg gtgaacgcca
    11461 cagaactatg gccgcccatc gagttgccca gcaggtggat tttggcgata tccagttgat
    11521 ccaccacgct tttcaggatt cgtgcattaa gatccgatcg tgaaccacta ttaacgatcg
    11581 aatcgctttt gccccaaccc ggacaatcca gcaggatcac ccgatagccc gcctctacca
    11641 gcggatcgat attgcggctg aagttcgccc agccagtagc acccgggccg gaaccatgca
    11701 gcaggacaac ggtttcgtcg ccttgtccgc agtcattaaa atggatgcgc agcgttttac
    11761 ccgcttcttc tacattcaga aaacggctgg tggcggcttc ggtttgtggc tgataactca
    11821 tcatcttctc ctgcatattc agttctctgt tctggcgctt aacgagccaa atccggcaat
    11881 ccactccggg attgggcggt aataacgccc ttcgctacgc cagttgccaa atgcagaaat
    11941 agcggcaaaa gcggcgaccc aggttttgat ttcatgtgtc gactttccgg caatggcgga
    12001 aagctcttcg ttactgacgg catccagttc ctgtatgcgt ccctgctcca gcaaagtcat
    12061 gaactggtta tcccaaatcg ggttgagcgg atgcagagtt ctctgatcct caacaaactt
    12121 ctcagcggcg ctaatcaccc tttgctgacg caattcgcgc tcactggcgg gtaaatcttt
    12181 cccgctcccc aacagacggt cgcgcatatg ggcatcggct ttcgccagtt cgggcaccgg
    12241 cggctgatgg gaaagcccac cggaacccag gaacagcacg cgtttattga gagtgctggt
    12301 gaaacgtcca atggcttcac ccaacatgcg ggtacgctgg aaaccgggca gcggcgtggc
    12361 gacaccgttg atgaacacag gcagaactgg caccttatcc agcccaccga gcaggaactc
    12421 cagcggctgg gcgaacccgt ggtccacctg catacagtaa gaaacggcaa gatcgatccc
    12481 gctcttcatg acagcatgcg cacaggcctc cgccagctcc acaggcacgg gcagctctcc
    12541 tgccgcactg ccgaaatcac caattgccgt cgctccaacg cctaaacaga acggtggcat
    12601 cacgtcatag aaaaagccgt tgtagtgatc tggcgcaaac agcaccacca gttcagggga
    12661 gaaagccgca atacgctcgc gggcgctggc aatcacgcca ttgacctcat cgagtacctc
    12721 ttgcgccggg tcgacatacc ccaccaacgg cgagtgggaa agacagtgaa gataagcgtg
    12781 catatcaggc taccttttcg acagaaacgt cggcatcagg gcgggtcagc gtcatcaccg
    12841 acgccagttt attcagcgta ttgcccaggg tttgcggaat ggcggtggcg gcaacaaagc
    12901 gatccgggcg catcaccacc agcgaagcat tgtgttgcgc gaaccagcta cgcagacgac
    12961 cttgcgtatc gcccacgtgt agcacgccgt cgtggttatc ctgtgcggta tgaatttgca
    13021 cttccggcac cacctgaatg aagcgtgtgc ccaacgcgcg ccactgctgg atttgctcgt
    13081 cgctcatccc ccacagtgga ttgcatcccc agccaattac cgcgaagttc gcgccgatcg
    13141 cgttatcgag cagcgtcacg tcgccgtttt ccagcgtgac tttcggctga ataaacatct
    13201 tgccgaccgg agagtgcttc gcttcgccct cacgcaccag cgcaccgccg taatattgcg
    13261 gcatcggctt gaagcgcatt tcgaggaagt agcgttttac tggcggcaga taattcaaca
    13321 gccaggaaac gccgtcacgt aacgtaccct gccagcgttt cggcggagcc agcacgttgc
    13381 ccgccgtcac ggacaggtca atcatcgctt tggcgtgatc gcgacgttct tgttgatagg
    13441 tatcgagcag cgcatcgcgg gctttcccct ggataaccaa cgccagtttc caggcgaggt
    13501 taaaggcgtc gcgcatacca ctgttatagc cctgcccctg ccacaccggc atgatgtgcg
    13561 cggcatcgcc cgccagcagt acgcgatcaa tacggaaacg ttgcgccagt cgcgcgttat
    13621 gggtgtagac acgctggcga atcaattcaa cattgtccgg attaggcagc actttgctta
    13681 acagcttgcg catattttgc ggctcacgca gctgctcttc ggtttctccc ggcatcacca
    13741 taaattcaaa gcgacgtacc gcatgaggca gcgcggcaga aacatacggg cgcaccggat
    13801 cgcaacacaa atagatatgc ggcgtactta acggatcgtt ggcgatatct accacaatcc
    13861 actgatttgg cgcagtttta ccttcaaacg gcacattcag ggtgcgacga acaaaacttg
    13921 ccccaccatc acaggccacc agccactggg ctttgatcgt ttcccgctgc ccttctgccg
    13981 ttttcaggtg caaggtcact tcgtcatctt gctggctgaa ggcctccagc tcgcgggaaa
    14041 acaagcagcg cacattcgga aaacgcgaca acccttccag catcaccgca tcgacctgtg
    14101 gctgaataaa ggcgttacgg cgcggccagc caaattcatc ggtcattggc tgaatatcag
    14161 caaaacagcg gccttttggg gtgagaaaac gcatcgcgtg ccacggtgta gtgtgcggca
    14221 gaacattctc gaccaggccg accgactgca tggtgcgcag cgcctcgtca tcaataccaa
    14281 tcgcacgcgg gtagtcgatc aacttatcga gtttctccac caccagcacg tcaatgccca
    14341 tctgaccgag atagttcgcc atcatcagcc caaccggacc ggcaccagcg atcgccacct
    14401 gaacgctatg gttaacagca ggctggatgt cagggtgttg tattgccatt tctgcacaac
    14461 gttgttttaa ctatagaaat gtcaattaat atgcagaaca atgagcagac ggaatacaaa
    14521 accgtgcgcg gcttaacccg cggtctaatg ttattaaata tgttaaataa acttgatggc
    14581 ggtgccagcg tcgggctgct ggcggaactc agcggcctgc atcgcaccac tgtgcggcga
    14641 ctgctggaga cgctgcagga agagggatat gtccgccgta gcccctccga tgacagtttt
    14701 cgtctgacca tcaaagtgcg gcaattaagc gaaggatttc gtgacgaaca gtggatttct
    14761 gcactggcgg ccccgctgct gggcgatctg ttgcgcgaag tggtatgacc aacagatgtg
    14821 tccaccctgg atgttgatgc aatggtggta cgcgaaacca cccaccgttt cagccgctta
    14881 tcctttcacc gggcaatggt cgggcgacgt ttaccgctcc tgaaaaccgc ctcgggcctg
    14941 acctggctgg ccttttgccc ggaacaagaa cgcaaggaat taatcgaaat gttagccgcc
    15001 cgccccggtg atgactatca actggcacga gaaccgttaa agctgcaagc cattctggcg
    15061 cgcgcgcgca aagagggtta cggacagaac taccgcggct gggatcagga ggagaagatc
    15121 gcctctatcg ccgtaccgct gcgcagtgaa caacgggtga ttggctgtct gaatctggtg
    15181 tatatggcga gcgcaatgac cattgaacag gcagcggaaa agcatcttcc ggcgctacaa
    15241 cgggtagcaa aacagatcga agaaggggtt gaatcgcagg ctattctggt ggccggacgg
    15301 cgaagcggcg tgcatttacg ttgacaccat cgaatggcgc aaaacctttc gtggtatggc
    15361 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgctata
    15421 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tctcgcgtgg tgaaccaggc
    15481 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
    15541 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
    15601 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
    15661 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
    15721 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
    15781 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
    15841 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
    15901 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
    15961 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
    16021 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
    16081 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
    16141 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
    16201 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac
    16261 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
    16321 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
    16381 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    16441 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    16501 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    16561 tgtgtgaaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    16621 gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    16681 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    16741 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
    16801 gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc
    16861 gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc
    16921 tatcccatta cggtcaatcc gccttttgtt cccgcggaga atccgacggg ttgttactcg
    16981 ctcacattta atattgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
    17041 ggtgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac
    17101 agccgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaaa aaaccgcctc
    17161 gcggtgatgg tgctgcgctg gagtgacggt agttatctgg aagatcagga tatgtggcga
    17221 atgagcggca ttttccgtga cgtctcgttg ctgcacaaac cgagcacaca aatcagcgat
    17281 ttccatgttg ccactcactt taatgatgat ttcagccgcg cggtactgga ggcagaagtt
    17341 cagatgtacg gcgagctgcg cgatgagctg cgggtgacgg tttctttgtg gcagggtgaa
    17401 acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt
    17461 tatgccgatc gcgtcacgct acgtctgaac gtcgaaaacc cggcgctgtg gagcgccgaa
    17521 atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg acgacggcac gctgattgaa
    17581 gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg
    17641 aacggcaagc cgttgctgat tcgcggcgtt aaccgtcacg agcatcatcc tctgcatggt
    17701 caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt
    17761 aacgccgtgc gttgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc
    17821 ttcggcttgt acgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat
    17881 cgtctgaccg atgatccgcg ctggttaccc gcgatgagcg aacgcgtaac gcgaatggtg
    17941 cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga gtcaggccac
    18001 ggcgctaatc acgacgcact ctatcgctgg atcaaatctg tcgatccatc ccgcccggtg
    18061 cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg
    18121 cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt
    18181 tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac
    18241 agtcttggcg gcttcgctaa atactggcag gcgtttcgtc agtacccccg tttacagggc
    18301 ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa tggcaatccg
    18361 tggtcggctt acggcggtga ttttggcgat acaccgaacg atcgccagtt ctgcatgaac
    18421 ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cagaagcaaa acaccagcag
    18481 cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc
    18541 cgtcatagcg ataacgagct tctgcactgg tcggtggcgt tggatggcaa gccgctggca
    18601 agcggtgaaa tgcctctgga tgttgctcca caagataaac agttgattga actgcctgaa
    18661 ctaccgcagc cggagagcgc cggacaactc tggcttacgg tacacgtagt gcaaccgaac
    18721 gcgaccgcat ggtcagaagc cggacacatc agcgcctggc agcagtggcg tctggcggaa
    18781 aacctcagcg tgacactccc cgccgcgccc cacgccatcc cgcaactgac caccagcgaa
    18841 acggattttt gcatcgagct gggtaataaa cgttggcaat ttaaccgcca gtcaggcttt
    18901 ctttcacaga tgtggattgg cgctgaaaaa caactgctga cgccgctgcg cgatcagttc
    18961 acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat tgacccgaac
    19021 gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag
    19081 tgcacggcag atacacttgc cgacgcggtg ctgattacga ccgctcacgc gtggcagcat
    19141 cagggaaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg
    19201 gcgattaccg ttgatgttga agtagcgagc gatacaccgc atccggctcg gattggcctg
    19261 acctgccagc tggcgcaggt agcagaacgg gtaaactggc tcggattagg gccgcaagaa
    19321 aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac
    19381 atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg
    19441 aattatggcc cacaccagtg gcgcggcgac ttccagttca atatcagtcg ctacagccaa
    19501 caacaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg
    19561 aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtttca
    19621 gcggaattcc agcttagcgc cggtagctac cattaccagt tggtctggtg tcaaaaataa
    19681 taataaccgg gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat tatgtactat
    19741 ttaaa
//
LOCUS       DAZRUF010000056        19712 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZRUF010000056 DAZRUF010000000
VERSION     DAZRUF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19712
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.056"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            92..1531
                     /gene="norV"
                     /locus_tag="ACHXOL_001617"
     CDS             92..1531
                     /gene="norV"
                     /locus_tag="ACHXOL_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR6808624.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            1528..2661
                     /gene="norW"
                     /locus_tag="ACHXOL_001618"
     CDS             1528..2661
                     /gene="norW"
                     /locus_tag="ACHXOL_001618"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR6808625.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(2789..5041)
                     /gene="hypF"
                     /locus_tag="ACHXOL_001619"
     CDS             complement(2789..5041)
                     /gene="hypF"
                     /locus_tag="ACHXOL_001619"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR6808626.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(5194..5721)
                     /gene="hydN"
                     /locus_tag="ACHXOL_001620"
     CDS             complement(5194..5721)
                     /gene="hydN"
                     /locus_tag="ACHXOL_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR6808627.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(5869..6879)
                     /gene="ascG"
                     /locus_tag="ACHXOL_001621"
     CDS             complement(5869..6879)
                     /gene="ascG"
                     /locus_tag="ACHXOL_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR6808628.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            7139..8596
                     /gene="ascF"
                     /locus_tag="ACHXOL_001622"
     CDS             7139..8596
                     /gene="ascF"
                     /locus_tag="ACHXOL_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR6808629.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            8605..10029
                     /gene="ascB"
                     /locus_tag="ACHXOL_001623"
     CDS             8605..10029
                     /gene="ascB"
                     /locus_tag="ACHXOL_001623"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR6808630.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(10143..10562)
                     /locus_tag="ACHXOL_001624"
     CDS             complement(10143..10562)
                     /locus_tag="ACHXOL_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR6808631.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(10559..10870)
                     /locus_tag="ACHXOL_001625"
     CDS             complement(10559..10870)
                     /locus_tag="ACHXOL_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR6808632.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(11032..11805)
                     /locus_tag="ACHXOL_001626"
     CDS             complement(11032..11805)
                     /locus_tag="ACHXOL_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808633.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(11830..12300)
                     /gene="hycI"
                     /locus_tag="ACHXOL_001627"
     CDS             complement(11830..12300)
                     /gene="hycI"
                     /locus_tag="ACHXOL_001627"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR6808634.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(12293..12703)
                     /gene="hycH"
                     /locus_tag="ACHXOL_001628"
     CDS             complement(12293..12703)
                     /gene="hycH"
                     /locus_tag="ACHXOL_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR6808635.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(12700..13467)
                     /gene="hycG"
                     /locus_tag="ACHXOL_001629"
     CDS             complement(12700..13467)
                     /gene="hycG"
                     /locus_tag="ACHXOL_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR6808636.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(13467..14009)
                     /gene="hycF"
                     /locus_tag="ACHXOL_001630"
     CDS             complement(13467..14009)
                     /gene="hycF"
                     /locus_tag="ACHXOL_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR6808637.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(14019..15716)
                     /gene="hycE"
                     /locus_tag="ACHXOL_001631"
     CDS             complement(14019..15716)
                     /gene="hycE"
                     /locus_tag="ACHXOL_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR6808638.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(15734..16657)
                     /gene="hycD"
                     /locus_tag="ACHXOL_001632"
     CDS             complement(15734..16657)
                     /gene="hycD"
                     /locus_tag="ACHXOL_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR6808639.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(16660..18486)
                     /gene="hycC"
                     /locus_tag="ACHXOL_001633"
     CDS             complement(16660..18486)
                     /gene="hycC"
                     /locus_tag="ACHXOL_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR6808640.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(18483..19094)
                     /gene="hycB"
                     /locus_tag="ACHXOL_001634"
     CDS             complement(18483..19094)
                     /gene="hycB"
                     /locus_tag="ACHXOL_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR6808641.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(19219..19680)
                     /gene="hycA"
                     /locus_tag="ACHXOL_001635"
     CDS             complement(19219..19680)
                     /gene="hycA"
                     /locus_tag="ACHXOL_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR6808642.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
BASE COUNT         4687 a         5499 c         5186 g         4340 t
ORIGIN      
        1 atagagtaaa aacaatcaga taaaaaactg gcacgtaatc tgcaattagc aagacatctt
       61 tttagaacac gctgaataaa ttgaggttgc tatgtctatt gtggtgaaaa ataacattca
      121 ttgggttggt caacgtgact gggaagtgcg tgattttcac ggtacggaat ataaaacgct
      181 acgcggcagc agttacaaca gctacctcat ccgcgaagaa aaaaacgtgc tgatcgacac
      241 cgtcgaccat aaattcagcc gcgaatttgt gcagaacctg cgtaatgaaa tcgatctggc
      301 ggatatcgat tacatcgtga ttaaccatgc agaagaggac cacgctgggg cgctgaccga
      361 actgatggcg caaattcccg atacgccgat ttactgtact gccaacgcta tcgactcgat
      421 aaatggtcat catcatcatc cggagtggaa ttttaatgtg gtgaaaactg gtgacacgct
      481 ggatatcggc aacggcaaac agctcatttt tgtcgaaacg ccaatgctgc actggccgga
      541 cagcatgatg acttacctga caggcgacgc ggtgctgttc agtaacgatg ctttcggtca
      601 gcactactgc gacgagcatc tgttcaacga tgaagtggat cagacggagc ttttcgagca
      661 gtgccagcgt tactacgcca atatcctgac gccgttcagc cgcctggtaa caccgaaaat
      721 taccgagatc ctgggcttta acttaccagt cgatatgata gcgacctctc acggcgtggt
      781 atggcgcgat aacccgacgc aaattgtcga gctgtacctg aaatgggcgg ctgattatca
      841 ggaagacaga atcaccattt tctacgacac catgtcgaat aacacccgca tgatggctga
      901 cgctatcgcc caggggattg cggaaaccga cccacgcgtg gcggtgaaaa ttttcaacgt
      961 cgcccgaagc gataaaaacg aaatcctgac caatgtcttc cgctcaaaag gcgtgctggt
     1021 cggcacttcg acgatgaata acgtgatgat gccgaaaatc gccggtctgg tggaggagat
     1081 gaccggatta cgcttccgta acaaacgcgc cagtgctttc ggctctcacg gctggagcgg
     1141 cggtgcggtg gatcgtcttt ccacgcgcct gcaggatgcg ggtttcgaaa tgtcgcttag
     1201 cctgaaagcc aaatggcgac cagaccagga cgctctggag ttatgccgtg aacacggtcg
     1261 cgaaatcgcc cgtcagtggg cgctcgcgcc gctgccgcag agcacggtga atacggtggt
     1321 taaagaagaa acctctgcca ccacgaagtc cgacctcggc ccacggatgc agtgcagcgt
     1381 ctgccagtgg atttacgatc cggcaaaagg cgagccaatg caggacgttg cgccaggaac
     1441 gccgtggagt gaagttccgg ataacttcct ctgcccggaa tgctctctcg gcaaagacgt
     1501 ctttgacgaa ctggcatcgg aggcaaaatg agcaacggca ttgtgatcat tggttcgggc
     1561 ttcgccgccc gccaactggt gaaaaatatt cgcaaacagg acgccagtat tccattaacc
     1621 ctgatcgccg ccgacagcat ggatgagtac aacaaaccgg acctcagcca tgttatcagc
     1681 caggggcaac gtgccgacga ccttacccgc cagacggcgg gtgaatttgc cgggcagttt
     1741 aatctgcacc tgtttccgca cacctgggtg acggatatcg atgccgaagc ccatgtggtg
     1801 aaaagccaga ataatcagtg gcaatacgac aagctggtgc tggcaactgg tgccagtgcc
     1861 tttgtcccgc cagtgcctgg gcgtgagtta atgctgacgt taaatagtca gcaagagtat
     1921 cgcgcctgtg aaacgcaact gcgggatgcc cgacgcgtgt tgattgtcgg cggtggtttg
     1981 attggtagcg aactggcgat ggatttttgt cgtgcaggca aagcggtcac gctaatcgac
     2041 aacgctgcca gtattctggc gtcattaatg ccaccagaag taagcagccg cttgcagcat
     2101 cggttgacgg agatgggcgt tcatctgctg ttgaaatctc agttgcaggg gctggaaaaa
     2161 acggattctg gcattctggc aacgctggac caccagcgta gtatcgaagt ggatgcggta
     2221 attgccgcca ccggactgcg cccggaaaca gccctggcac gacgcgccgg gctgacgatt
     2281 aatcgcggcg tttgcgtcga tagttatctg caaaccagta atgacgatat ttatgcgctg
     2341 ggcgattgcg cggaaatcaa cggtcaggta ctgccattcc tgcagccgat tcaacttagc
     2401 gcgatggtgc tggcaaaaaa tcttctcggc aataacacgc cgctgaaact accggcgatg
     2461 ctggtgaaaa tcaaaacgcc ggaattaccg ctgcatctgg caggcgaaac ccagcgtcag
     2521 gatttacgct ggcaaattaa taccgaacgc cagggaatgg tggctcgcgg tgttgacgat
     2581 gctgaccagc ttcgcgcctt tgtggtcagt gaggatcgga tgaaagaggc atttggattg
     2641 ttgaaaacat tgccgatgta ggtgggctac tgtgcctaaa atgtcggatg cgacgctggc
     2701 gcgtcttatc cgacctacgg ggacgcatgt gtaggccgga taaggcgttt acgccgcatc
     2761 cggcaatggt gtccaaatgc aacacgtttt atccgttctg gacttcaccc gctaaccaac
     2821 gcgccgcagc aataaccccc tgccccagag acaaaccgcc atcacccgcc ggtaaactct
     2881 gtggaaagag caatgtgaaa tcagcgagat aatgcgccag acgtgcacgc agcaaacggt
     2941 tatgaataac cccgccgcta aataccagcg tagtgatacc acgcatcgtg gcctgctcac
     3001 gcatcaacgc ggcaaaaccc tgcgccagcg catcatgaaa cgcccacgcg cgttgattaa
     3061 ccggtgcctg ccagttcagc cactgctgcc agaaagcggc gagatccagt tgattgtcca
     3121 ccagcggcat cgtcaccgga tgcgtcactc cgtggcatga ggctgcgagc gcctccagag
     3181 cacaagccgc ttcaccttca taacttaacg tggctggcgc acagcccagt gccgccgcca
     3241 ctgcatcgaa caaccgccca cacgatgacg ccagcggcgc gttaattcca cgctcaatgg
     3301 cccgcgccag cacgctccag ttttgctgtt gcacacttgc tgtttcaggg taattctgcc
     3361 actccggcac aaagcgcagg cactgcgcca gcaggtttcg ccacggctgc ttcgctgcca
     3421 aatcgccacc cggaagcgcc actgcaggca agccgcccag gtgctcacat tcgcgatagt
     3481 tcacccgcag gcactcgccg ccccacaaag cgccgttctc ccccataccg ataccgtcga
     3541 gcgtcaaagc aatgacatca ccgccatcca acggccactg atgctctgcc agacacgccg
     3601 ctgcatgggc atgatgatgc agtaccgttt gcgtcggcag attcatttca cgcgcccact
     3661 ggctggagac atagcccgga tgcgcgtcat gcacaacgta ttgcggggta aaatcgtaga
     3721 tgttttgcat caggcgtaac gcttcgcgcc actgcatctg gatgccatcg tcacttaaat
     3781 cgcccagatg ctgactcaac accgcttgtt caccgcgcac caggcagaag gtatttttca
     3841 gatccgcgcc gagacacagc acaggcggaa catttttaaa gcccggaggc aaagccagcg
     3901 catccggcac atacccccgc gaacggcgca gcatttcgcc gctttcgcgc accaccgaat
     3961 catccatccg ctgcacgatg tcgcggttat gtatcaagaa tccgtcggca atgccctgca
     4021 aatccgccag cgcctgttcg ttgctgatag ctggtggttt accgctcagg ttgccggagg
     4081 tcatcaccag cgggcattgc agttcctgta acagcaaatg ctggagcggg ttcgcaggca
     4141 acattacccc gacttcgtta aggtcagggg cgatatcatc acaaagctca ggaacgtatt
     4201 ttttatccac cagcacaatc ggcgcggcgg gcgtggtaag caactggcgc gcagcgtctg
     4261 gtaaaccgtc agccactggc aacatgaccg ccagcggttt cgccgggcga tgtttgcgcg
     4321 cccgaagtgt cgccaccgcg ttactgttac gtgcatcgca ggcaagatga aatccgccaa
     4381 tccctttgat ggcgacaatg ttgcccattt ttaactgtgc gatagccgcc tgtaatgccg
     4441 cctcttgttc tgcatgttca ccatgactta cccattcaag atgtgggcca cactccgggc
     4501 aggccaccgg ctgggcgtgg aagcgacgat cgagcgggtc acggtactct ttgtcacaag
     4561 ccggacacaa cggaaacgcc gccatcacgg taaacgggcg gtcgtaaggc atggcgcgaa
     4621 taatggtgaa acgcgggccg cagtgggtac agttgataaa cggataacga taacgccgtt
     4681 cgcctggggt attcatttcg gcaaggcaag cagggcaagt agcggcatcc gggacaattt
     4741 gcgtattcat ggcacctccc gcgctctggc ggatggtgaa ctcagtgggc agttgtgacc
     4801 agatgtacgg ctcacgctcg acgctatcaa tacgcgccag cggcgggcag tgctgatgca
     4861 attgaacaag taacgtttcc gggtcttccc gcagccggac ttctacgcca tcgccgtcat
     4921 tacagacatc gccgtgaaga tttaattgct gtgccagctg ccagacaaac ggacgaaaac
     4981 cgacgccctg cactttgcca cgaatacgca gttggacacc gcaagatgtg ttttttgcca
     5041 ttgagttatt cccgccatca tgaattgcgt aacccgtcct gccggacacg actgcgtcgt
     5101 atccggcagt ctcaggtcgg cgataccgcc agctccgtat tctacgaata tttccgggaa
     5161 ttcctttgat gccagaacag ttctgtaaga tttttagaac atcagcgccg tacggcggcg
     5221 tttttctgca ctcagttgtt caagtttatt acgatcgaca caaatcagcg catgagtcgg
     5281 gcaagccgcc atacacgccg ggccgtcttc acgatggttg cacaggtcac atttattggc
     5341 ttcggctttg tcagcccgta cattcagccc cgcgccgctg ttgcgaatca ccggacgtac
     5401 caccacttcc atcgcgccat acgggcaagc cacaacgcag gttttgcaac caatgcaacg
     5461 ttcctgcatc acatgaacaa accctttatc acggctgata gcaccattcg ggcagacgtt
     5521 agcgcacggt gcatcttcac actgacggca aactgtcgcc gtggaaatgt tcacaccttt
     5581 aatgacatgg atacgcggta aaaatgtttc cggggtcagc gatgcacagt cctgattttc
     5641 ctgatgagaa accacgcacg ccacttcaca ggtacggcaa ccaatacatt tactcgcgtc
     5701 agcaatgatg aaacggttca tcaaattctc cagcaatgac agttaatgcg ccgatacatt
     5761 cacaaatcat gccagtttta attcactgtt atttaaggaa attaatttct gtaatgcagg
     5821 aaaaaagatg tcatcgacac tagtgacgat gacatgtgat gacaatgttt atcgcgaaag
     5881 agcaatgagt gagtcgcggc ggatcagttt tccgctgaag gttttcggcg gtgagaaatc
     5941 cccgccatcg agcataaaaa tcagccgtcc aataatttcc tgaatcatct cagtcaccgg
     6001 aatttttacg ctggagagcg ccggaacgat atagggggca attgcgatat catcgaatcc
     6061 gataactgac acctgctctg gcaccgctac gccgcgctcg tgcaacgctt tcatcgcacc
     6121 tatcgccata tcgtcgttac tggcaactaa cgcgctaaat ttagccccac gttcgagcaa
     6181 cgtttctacc ccttcggccc cgctggcggg cgtccattta ccgttagcga taagtttttc
     6241 attgagcgca ataccatgct gcgccagcgc gtctttatac ccagcaagac gttcaatgct
     6301 ggtgggggaa tccatcgagc cggtaaggaa agcaatctcc tgatgtccgg cgtttatcaa
     6361 ctctgccacg gcattaaagc tggtctgttt atgatcgcac cagacgctat ggctgctgtt
     6421 tttgcgcagg cggcgattaa gcaccattat cggctgactg tgcgcgtcaa tgatgtcatc
     6481 gatctcatcc acgcttaaaa aacgcgggta aatcataatg gcgtcgcagc gcaaatccag
     6541 cagatactga atcgcctggc gctcttcttc ggcgctgtgt ttaccatctg ccaatagcaa
     6601 ctgccgccct ttctcttccg ccattcgcgc ggcatgaaag agtaattcac taaaataaat
     6661 gccgtggtaa agcgtgttgg tcactaccaa ccccagcgtc tgagtactct tcgccgacag
     6721 attgcgcgcc agcaagtttg ggcggtaacc gctctcttct accgcctgaa acacgcgatc
     6781 tttagtctcc tggctgacgt agccattacc tgaaagcacg cgggaaacgg tcgcttttga
     6841 aaccccggcg cgcttcgcca cttccagcat cgtcgtcatc attttcatcc ctttacacgc
     6901 aatcaacgca gtgtactgca ccgtttgccg attgtccttg cgcaatcggc gggaaaaata
     6961 ttcaggtgac cggtttcaca aatataaaaa atgaacaatt aactctcttg cttatttagt
     7021 gacaactatt catgattttg tgaaaccggt ttcctaattc cgtttcagca tcggcatttt
     7081 tccgttacgt cgactgataa caactacatc taccctactg ataacaggat aaaatccgat
     7141 ggccaaaaat tatgcggcgc tggcacgctc ggtgatagcg gcactgggcg gcgttgataa
     7201 catctcggcg gtcacgcact gtatgacgcg tttgcgcgtt gttatcaaag atgaccagct
     7261 tatcgacagc ccgacgttaa aaaccatccc cggcgtgctc ggcgtggtac gtagtgacaa
     7321 ccagtgtcag gtgattatcg gcaataccgt ttcacaagcc tttcaggaag tcgtcagcct
     7381 gctgccggga gatatgcagc ccgcacagcc cgtgggtaaa cccaaactca cgctgcgtcg
     7441 cattggtgcg gggatcctcg atgcgctgat cggcaccatg tcaccgctga tcccggcgat
     7501 tatcggcgga tcgatggtca aactgctggc gatgatcctc gagatgagcg gcgtgctgac
     7561 aaaaggatcg ccgaccttaa ccattctgaa tgtgattggt gacggtgctt tcttcttcct
     7621 gccgctgatg gtcgcggcat ctgccgccat caaatttaaa accaatatgt cgctggcgat
     7681 tgccatcgcg ggtgtgctgg tacatccgag ttttattgaa ctgatggcga aagcggccca
     7741 gggtgaacat gtcgaatttg ccctgattcc ggtcaccgcg gtgaaataca cctacacagt
     7801 gatcccggcg ctggtgatga cctggtgcct gtcatatatc gaacgctggg tggatcgcat
     7861 tacaccggcg gtgaccaaaa acttcctcaa gccaatgctg attgtgttga ttgccgcacc
     7921 gctggcaatc ctgctgattg gcccgattgg tatctggatc ggtagcgcca tttcggcgct
     7981 ggtttacacc attcatggtt atctgggctg gctttcagtc gccattatgg gcgcgctgtg
     8041 gcctctgctg gtaatgaccg ggatgcaccg cgtctttacg ccaaccatca ttcagaccat
     8101 tgccgaaacc ggcaaagaag ggatggtcat gccgtcagag atcggcgcta acctgtcgct
     8161 gggcggttca tcactggcgg tggcgtggaa aacgaaaaac ccggaactgc gccagacggc
     8221 gctggctgcg gcggcatcag ccattatggc ggggatttcc gaaccggcgt tatacggcgt
     8281 ggcgatccgc ctgaaacgtc cgcttatcgc cagtcttatc agcggtttta tttgcggcgc
     8341 ggttgccggt atggcggggc ttgccagcca ctcaatggca gcgccagggt tatttaccag
     8401 cgtgcaattc ttcgatccgg cgaatccaat gagcatcgtc tgggtgttcg cggtcatggc
     8461 gctggcggtg gtgctgtcgt ttatcctcac actgttgctc ggctttgagg atattcctgt
     8521 tgaggaagcg gctgccgagg cgcgaaagca tcagagcgta caaccgaccg tcgccaaaga
     8581 agtaagtctt aattgaggat gaaaatgtca gtatttccag aaggtttttt atggggcggc
     8641 gcgcttgccg ccaaccagtc tgaaggtgcg ttccgtgaag gtggcaaagg gctgaccact
     8701 gtcgatatga tcccacacgg cgagcatcga atggcggtga aactggggct ggaaaaacgt
     8761 tttcagttgc gcgatgacga gttttatccc agccatgagg cgacggattt ttatcatcgt
     8821 tataaagaag atatcgccct gatggcagag atgggattca aggttttccg tacctcaatt
     8881 gcctggagcc gtctctttcc gcagggcgat gaaatcacgc ccaatcagca gggcattgct
     8941 ttttatcgtt ctgtctttga agagtgtaaa aagtacggta tcgaaccgct ggtcacgttg
     9001 tgccacttcg atgtgccgat gcatctggtc accgaatatg gctcctggcg taaccgcaag
     9061 ctggtggagt ttttcagccg ctacgcccgg acctgctttg aagcatttga tggtctggtg
     9121 aaatactggc ttaccttcaa tgaaatcaac attatgttgc atagcccgtt ctccggcgcg
     9181 ggtctggtgt ttgaagaagg tgaaaatcag gatcaggtga aatatcaggc cgcgcatcac
     9241 cagctggttg ccagtgcgct agccaccaaa atcgcccatg aggttaaccc gcaaaatcag
     9301 gtgggctgta tgctggcggg cggtaacttc tacccttaca gttgcaagcc ggaagatgtc
     9361 tgggcggcgc tggagaaaga ccgggaaaac ctgtttttta tcgatgtgca ggcgcggggc
     9421 acgtatccgg cttactctgc ccgcgtattc cgcgaaaaag gggtaaccat cgacaaagca
     9481 ccgggcgatg atgaaatcct gaaaaacacc gtcgattttg tctctttcag ctattacgcc
     9541 tcgcgctgcg cctcggcgga gatgaacgcc aacaatagca gtgcggcgaa cgtagtgaaa
     9601 tcgctgcgta acccgtatct gcaagtgagc gactggggct ggggtattga tccactcggt
     9661 ctgcgtatca ccatgaacat gatgtacgac cgttatcaga agccgctgtt tctggtggaa
     9721 aacggcctcg gcgcaaaaga tgaacttgct gccaatggcg agattaacga tgactatcgc
     9781 atcagctatt tacgcgaaca tatccgcgca atgggcgaag cgattgcaga tggcattccg
     9841 ctgatgggct acaccacctg gggctgtatt gatttagtct ccgcctctac gggtgaaatg
     9901 agcaaacgct acggttttgt ctacgtagac cgtgacgacg caggcaacgg cacgctgacg
     9961 cgcacacgta agaaatcgtt ctggtggtat aaaaaagtga ttgccagtaa tggggaagat
    10021 ttagagtagg taacagtgcc ggatgcggcg tgaacgcctt atccggccta catgtcgcac
    10081 gaaccaggaa ggcccgataa gcgcagcgca tcgggcaatc tggggtttgg catcagtcaa
    10141 aatcaaagaa accattccgg cttaacgcca aatcgggctg aaagtgcctt aatgtgggat
    10201 atcgtcaggg atcgttgtcc agacaaaatc tgactgacca gggatttaga gccgatttcg
    10261 ttcttcaaat ccgcataggt cagattatgt tgatcaatca gcgtgcgaag taacgccacg
    10321 ccaacaggca ttgccgcaac ggctttgtcg aactctgcaa actcttggtt gttgttttca
    10381 tattctgcaa tgcggctcgc cagaaagtcg ataagtggat tggtgtcatc ttcttcaatc
    10441 aaatattcga ccagttccag tgcttcacga taatcatctt ctgagtcact acctcccaga
    10501 aaggggactg cattgaccag ttctcttgtt gctttaacag cgcgtgcagc attagcagtc
    10561 attctttgtt ctctctgtag taacgtgtca gtttgtcata ctcggcgtga ttagttatgt
    10621 gcttcacaaa gaaccgctta ttaacaaagt ttatgtaggc aatcaccctt aggttgttcc
    10681 cacctacatc cagaacgtac catttgttgc gatatttaaa attgtcgagg ttgggaaacg
    10741 ccgttcgcat ctcttctggc gtagaaaaat ccgtttcctt aataacccga tacaatgcct
    10801 gaagagccag agcgtcgtta ggatatttac gcgcagactc ttcaaatggc gctttagaaa
    10861 tgatgtgcat ttttaagttt taacttcctt atagtaagta aacagccaca tctgcgagtt
    10921 ctatccttat gtgttcggaa actatagctc atcaaatcca agttcacaat atgtaaacac
    10981 ttgagcgcga tataattacc cccaaaatcc tcatagccac tctgcaaaaa gtcagttctg
    11041 gttcacccca tcaagaacat ccccctttat caagcaacgc tgcgtctgcg ccagatcttc
    11101 cgtttgcggg tcgcgcccgg cgatgggttt gaggacgagt ttacggttgg gggtataagt
    11161 aaaacgcgtg agcgcgggat agcttaactt gccaaaccag ccgtgaacac gctgccattt
    11221 ggcgtctttt acctgataca gactggttat ccagtaaccg gaatcaaact gcatatccag
    11281 caattgcgtg tgaccattgc cttgcaaatc cagcaaatca tctacgccag ttttgcttgc
    11341 ggtgtagaat cccctgggtt caaagacaca gggacggcct tcgcgcgtga acgtcataag
    11401 aattaaatgt gcataaggag ccagaccatt accggtactt ggtaaccaga taaccagatc
    11461 ctgaatgccg ttacgatcaa gatcggcgcg atagagttgc cctttgcccg ctatgtttgc
    11521 cagcataccc acaggcacag accaggattt accggcgcga tctttaccgt tcaattgcca
    11581 ttcactatca tcatttggca tcttttcaag cgtcacgcca gcaacgccca cttttctaaa
    11641 atgccaggtt tgctcggcat cataaggcat ggtcaggaga ggtttcagcg cccgcttttg
    11701 cgcgtattcg ctgtcactca ttggcagata ttcggtaaag ctggtggcct gtgcaggcag
    11761 cactaagctc aataaaaaag cgcacatccc tgttctgata ttcatttcgc tgaattccct
    11821 gatggagaat tattcttctt ccaccgctaa ctgcgcgaag ccgccattcc cttcccagcc
    11881 ttccagtcgt tgataaacgg tttctaccgc atctttaatc ggctgagtca tcgggtagta
    11941 aaagccgacg atatccggct ggatgccgag gaaaatcact tcgccaatgt cttctttcaa
    12001 ctggtcgata agatagttca gcggcatgtt atgggtagtc atcataaaca tctcggcgat
    12061 atcatccggg tcgatgatgc ggatttcgcc ggggtttagc cccatatcag tagcgtcaac
    12121 aatcagtaac cgcgtagggc gcagttcacg gatggcgacg atgtcgtttt ccggtgcact
    12181 accgccatca atcaccaccc agttaccttt cggcgcggcg gcgcactttt ccgccagcag
    12241 cgggccagcg ccatcatcgc ccatcatgct attgccaaca cagagtaaaa cgtcagtcac
    12301 gcagtctcct caccatcaaa tagatggcgt tttcctgatg aatatcatgc agcatactta
    12361 acagcattgt actccattcc ttctgctgcg aagtttgcgc tgcccgggct ttatcgaatg
    12421 cgttggccag catcggcaca tggttaatgt cgatgacgat ctcaccatat ttcggcacgc
    12481 cttccatttt gcggcgggct tcactgcccg cctccagagt ggcaatccat gccagatatt
    12541 catcccacgg gcaggtgagc gccgcttcca ggcaatcgat aaccccaagg tggtgaccaa
    12601 tcgccaggct gtaatagacc acctgctgcg cctcggcggg cgtggcatcg ttctcatcaa
    12661 taaatttacg gctcagttga ctgaacacca ccttttcact catcggatac gcgcctcttc
    12721 aacaacatga ttcagatggc tgacaatctc gttcaggcgc ggatcgtttt ccgcttccag
    12781 ccagcgtgca acctgttctt cgccctgccc taactgtgtc aggaagtcat cggcaatctg
    12841 acgaccgtaa cgataacccg ccaggcggcg tgcttcgcga tccactttca cacgcagcgg
    12901 ctgcaccata tcgccatgca ggatttccgc ctgttgttca tcctgctcac ccgggccacg
    12961 ggcgtgaatt ttctgctcca gcaggccgag cgccattgca aagccgtaca gcgtggcggc
    13021 aggcgttggc gggcaaccag gaatgtagac atctaccggg acgattttat ccgtaccgcc
    13081 ccacacgcag tagagatcgt ggaaaatacc accgctgtta ccgcaggcac cgtaggagat
    13141 acaaattttc gggtccggcg cggactgcca cgcacgcagc gcaggggatc gcattgcacg
    13201 ggtgaccgcg ccggtaaaca gtaaaatatc cgcatgacgc ggtgaaggaa cgactttaat
    13261 gccgaagcgt tctgcatcaa acagcggcga aagcgtggcg aaaatttcga tttcgcagcc
    13321 gttgcagccg ccgcagtcca cgcgataaac gtaggcagaa cgtttgattt ttttcagtaa
    13381 cgacgccttc atgctggcga tggattcatc caccgtcatc gggaccggaa tgccgttggc
    13441 gtcacggggg cctaataaat tgctcatcag atggcctctt tcatatggcg agtcagttca
    13501 atacggtcgg acggcaccag gcatttctgg cgcttacatt ccgggcaagt ctcaaagctt
    13561 tcgcggtggt tttccgcgcg gctgtcgccg ttgtgcttaa gcagcgcaat ggcgtagtcg
    13621 atctctttct ggacggcgaa aggacgattg cagacgcggc agttgcacag cgcgaagcgg
    13681 gactgttgca ggaagtcttc tttcttccac actgccagtt cgtactcttg cgacagtttg
    13741 atcgccgccg tcgggcaaac ttcttcgcag cgtccacaga agatgcagcg cccgagatta
    13801 aactcccacg ccagctcacc ggtggcgagg tcagtttcaa ccgttaaggc gtttgacggg
    13861 caggcattga cgcaggccgc acagccgatg cactgctgcg ggttctgctc cggcttacca
    13921 cggaagtttt tatcaaccgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt
    13981 ttgatgactt ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta
    14041 cgctcaatgc tgtaacgctc gagttctttg tacggcacca ctttgctctt tttcttacgc
    14101 acatcgacca cggtcatgcg gtcggtacag gagtagcaag ggtccaagct accgataatc
    14161 agcggcgcat cggaaacggt gttaccgcgc agcatgtagc gcagggtcgg ccagttcgcg
    14221 taggtcgcgg cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag
    14281 tggatatcgt cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag
    14341 gtaaagcctt ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcatg
    14401 ttcagcgcgg tataaacttc gttgatacgc actttcagac gggaaataac gtcgcagccc
    14461 tgctcgctgt ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga
    14521 gtatcgcggg cgtgaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcc
    14581 atttccgggt ccagacgacc aatgccgacg gtgcgctgtt ccatgttcgg cgtactcagc
    14641 agcacatcca ccagctcctg cacttcacga cgcatctgtt gcgccagctg gcgggtctgg
    14701 atcatgtcat ctttcaacag atcgcgacga atcccgccga tcaggttcag gccgtaggtt
    14761 ttacgcgcac cggtgaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac
    14821 tgcataaagc cggagtcaaa gccggtaaag tggcaggcga gaccgaggtt gagcagatgc
    14881 gagtgcaggc gttccacctc cagcagaatg gcgcggatca tctgcgcacg ttctggcacc
    14941 tgaataccca tcgcgttttc taccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag
    15001 atcccgcaca cacggtcaga gaggaaggtc acttcgttgt aacccatacg ggtttccgcc
    15061 agtttttcca tgccgcgatg aacgtagaac agacggtagt cggcgtcgat aatgttttcg
    15121 ccatcgacga acagacggaa gtggcccggt tcgtcagaag tgacgtgcag cggaccaatc
    15181 ggcacgacgt tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg
    15241 gtggtcggtg ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca
    15301 tccggcagca ccagacggcg ttcatccggc agaccaaccg gaatcaaacc gtacatatcg
    15361 cgcacttcgc gctcgcccca caccgccgcc ggaacgcgcg gcgtcacgga cggatactcc
    15421 ggtttgttgg catcaacttc gacgcgaacc gttacccagc acttagtccc cttctccatc
    15481 gacagcacgt aataaacggc gtaatgacca ttcagtttgc gttcgtcgtt accaaaaagc
    15541 accgacaacc agcccccctg cttgtagtag agaaactcca ccacttccgg cagatagttc
    15601 acctttacgg tgacagtcag ctgatctttg gtctgccagg cgtggtccag cacgacgccc
    15661 ggaaatgcct cattcagcgc ggcgagataa tgttgaccta atttttcttc agacatgctc
    15721 aaactctctt taatcacgcc gccagcaagg agacgaacgc taaaaatgca aagccaaacc
    15781 cagtccaggt aatgcgcgga gtaatatcaa gacgcagacg ggccatgctg ttttcgaaca
    15841 gcgcgataac caggacgccg acgaccagtt tgacgatggc aatcaccagc gccagcagca
    15901 gcccaccggc ggtgaaggtt tccatctgtc cccacgggat aaatacgccg acgaacatct
    15961 gcagcaccac cagctgtttc aggctgatac cccatttcat gacgccaaag ccgctgccgc
    16021 tgtattcaga gagcgggcct tcctgcagct cctgctcggc ttccgccagg tcgaacggca
    16081 gtttgcccat ttcgataaag gtggcgaacg cacaggcaca aagcgccagt accagcggga
    16141 tgctctggct cagcggccag tgataaacgg tgtcggtgat gttgctgatg ttggtggaac
    16201 cggcaacctg tgcggcaacc cacagaccaa gcagcagcat cggttcgacc agcacgccaa
    16261 gcatcgcttc acggctcgcg ccgatagcgg taaacgggct accggtatcc agaccagaaa
    16321 tggcaaagaa gaaacgcgcg atggcaaaga gatacagtaa ggtgattaaa tcacccagtt
    16381 gcggcagcgg agaaccgacg gtcaccaccg gcagcgcagt agcgatagtc agcatgacgc
    16441 ccaccatcac atacggcgtc aggcggaaca cccagccgga ggcatccggg ccaacgctct
    16501 ggcggcccag cagtttgaaa atgtcacgat actcctgcaa cacacccggc ccacgacggt
    16561 tatgcagacg ggcgcgcgcc acgcgggtaa taccggagag cagcggcgca acggcaaata
    16621 acaccagcgc ctgaattaac ggatataaaa cactcattct caggctcctc gtgaaacaat
    16681 aatcaccacc agtaccgcca gttcaaccag cgccatccgg cggaacagca acgcactacc
    16741 ttcgcactgc cagcccggca ccagcgacac cggattcagc catttgcgca gtttcagcac
    16801 tggcgcaaac gcctgtttaa ccggcatggc aaaaccgtga gcggtaatca ccattgattt
    16861 ttcatggtcg tagccgcaca cccaggccgc accgcgggaa cgtgacggca ggcggtcgcc
    16921 tttgcaaatc gccataatga tgaatggcag cagcgggcag gcaatcagca gcaatgtgat
    16981 cattggttga gaaacggtgg tgttagcagg ctccagaggc agaggtacag cagcagagag
    17041 catcggcagc agccacggcg cagcaacacc gccaattacg cagcaaatcg ccagtgccac
    17101 tacgcttacg ctcatcagga gcggcgcaca ggtggcgttt tcggcttctt tggtacgcgg
    17161 cgcgccgagg aacgtgacgc catagacttt cgccatacac atcaccgcca gcgcaccggt
    17221 aattgccaga cccacagcga gcagcggccc cagcagacgg gcaacaaacg cgccgctatt
    17281 gctcagtttg aagaaggatt gatagataac ccattccccg gcaaacccgt tcagtggcgg
    17341 cagcgcagcc attgccatca acccgactaa catggcgatg gagataaccg gcattttctt
    17401 gccaatacca ccgagttttt cgatatcgcg atgaccggta cggaaccaga cgctccccgc
    17461 cccgaggaac agtacgcttt tgaacaggct atggttaagc agatggtaca gaccaccgac
    17521 caggccaaga gcaatcagcg ccggttgttc gagcgcgata cccgttacgc cagcgcccag
    17581 ccccagcagg ataatgccga tattttccag ggtgtggtaa gccagcaggc gctggatgtt
    17641 gtgttccatc aacgcataca gaccaccgac gaacgcggtg atcatgccga gcaccagcag
    17701 cgcgatcccc caccacagcg gtgcattacc gcccagcagt gacagggtta acatgcccag
    17761 caggccaatt ttcatgacta ccgtagaaaa caacgcggca gctggcgcag aggcgttagc
    17821 atgtgcctgc ggcacccagc cgtgcagcgg aataatcccg gccagcaggc caaagccaat
    17881 cacaccgagc agccagatat cggaaccgag cggcagctgt tgcatacgca tatccagtag
    17941 gcacagatcc agcgtgccat aacgctgcca cagcagccag caagcaatca ccagcagcag
    18001 agtgccaaga cgccccagcg caaaccacag tttgccctct ttgctgttgc tggtgaggaa
    18061 caccgcacac aaggccatga cttcggccat taccacgaac atgccgaggt tgctggcgat
    18121 gacggcgcag acggcggcag ccatcaacat attgatctgc aagccgttgc acttcacctg
    18181 cgcgtggcga tgccagtcaa tgttgtagag gctgacaaac agaccgcaca gaccgagcgt
    18241 aatcagccaa atcgcgttaa gcggagagat ttgcacatcg tagcttacca gcgacagcgc
    18301 accgctcacg ccaaccgcgc cagtcagtac agtgaagccc gcggctgccg tatacagact
    18361 accaaccgcg ccgccaattc cagctatcca gccacttaac gctttttgaa aagaaaagag
    18421 aaatgccaga acagcggcgg cgacaaacca tgccacgccg ctattgatca gggaaattgc
    18481 gctcatttag cctctccact ttgagcctgc tgaaacaagg tgagatcgcc aaagtccgtg
    18541 ttaaaggtca gctcacgctt acgtttgctg acgcgggcga tatcggtgtt atccaccaga
    18601 tgcagggctt tagtcgggca catccgcacg caggccggac cttgttcatc aaaactgcaa
    18661 aggtcacatt tgacggcgat cgcgcgaata cccggcaccc agtcaagcag cgtactgata
    18721 cgcgccggag caggcggtgc cggtggcgct ttcggggtat tggcgtttgc cggaatatcc
    18781 agcggacggc tgccggaaaa ttcaattgcg ccaaacgggc aggcgatgcc gcacagtttg
    18841 cagcttacgc acagactttc attcaactgc acggccccat caatgcgggt gatggcgtta
    18901 accgggcaaa ccaccgcaca gggtgcatct tcacagtggt gacagagctg cggcgcagat
    18961 tctttttcat tcagcatcac tctcaggcgc ggcattgatt gcaggccgtg ctggcgatgc
    19021 gtctctgaac aggcggcctc acaagtgtgg cagccgatac agagcgtgga gtcaacaatt
    19081 acaaaacgat tcaccaggca ttcctcaggt gattgtcatt tttgacgaaa acatgccgat
    19141 gaaatgtcat tttcgacact catcgacacg cccatcccca aacaggcgta acgcctgcaa
    19201 aacgggcaaa gcctcagctc atgctgccgg gctttgtccc tttaccagtt ggcttaaatt
    19261 caccggcaca ttgttttcaa tagcggtgta taagctgtcg taaacgccag cgattttttc
    19321 gagatagtgt tccagaacct gttcgcgatc gcggatgttg accttgccat caatcatgcc
    19381 gtcaatgctc agttgcgcct gcgcaatccg ctgtttgtct tcgccatctt ttgtttcgac
    19441 gtaatactcg atggtgtggc tgttaaagcc atccgccagg gtgacgtaaa tacgaaaatg
    19501 ttcaaaaagg acgaaacaga gttctttgtc cggcgcgcag ctcatggcgt gatgcaggcg
    19561 cgctttcgat aacgtgatcc cctgaaccag cgaattgcag tagatgtgcc actggtcctg
    19621 taggcgacga tgccgctgtg cgatgtaatc ggctttctcg cttatttccc aaatagtcat
    19681 tgtcaggtta cccgtttaac agagatgcca gc
//
LOCUS       DAZRUF010000057        19639 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZRUF010000057 DAZRUF010000000
VERSION     DAZRUF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.057"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            255..755
                     /gene="tssB"
                     /locus_tag="ACHXOL_001636"
     CDS             255..755
                     /gene="tssB"
                     /locus_tag="ACHXOL_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR6808643.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            790..1014
                     /locus_tag="ACHXOL_001637"
     CDS             790..1014
                     /locus_tag="ACHXOL_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808644.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1065..2540
                     /gene="tssC"
                     /locus_tag="ACHXOL_001638"
                     /pseudo
     CDS             1065..2540
                     /gene="tssC"
                     /locus_tag="ACHXOL_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2547..2960
                     /gene="tssE"
                     /locus_tag="ACHXOL_001639"
     CDS             2547..2960
                     /gene="tssE"
                     /locus_tag="ACHXOL_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR6808645.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2964..4814
                     /gene="tssF"
                     /locus_tag="ACHXOL_001640"
     CDS             2964..4814
                     /gene="tssF"
                     /locus_tag="ACHXOL_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR6808646.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4778..5860
                     /gene="tssG"
                     /locus_tag="ACHXOL_001641"
     CDS             4778..5860
                     /gene="tssG"
                     /locus_tag="ACHXOL_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR6808647.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5885..7165
                     /gene="tagH"
                     /locus_tag="ACHXOL_001642"
     CDS             5885..7165
                     /gene="tagH"
                     /locus_tag="ACHXOL_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR6808648.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7162..7686
                     /gene="tssJ"
                     /locus_tag="ACHXOL_001643"
     CDS             7162..7686
                     /gene="tssJ"
                     /locus_tag="ACHXOL_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR6808649.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7689..9020
                     /gene="tssK"
                     /locus_tag="ACHXOL_001644"
     CDS             7689..9020
                     /gene="tssK"
                     /locus_tag="ACHXOL_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFR6808650.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKIFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPCIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9025..9786
                     /gene="icmH"
                     /locus_tag="ACHXOL_001645"
     CDS             9025..9786
                     /gene="icmH"
                     /locus_tag="ACHXOL_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR6808651.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9795..12584
                     /gene="tssH"
                     /locus_tag="ACHXOL_001646"
                     /gene_synonym="clpV"
     CDS             9795..12584
                     /gene="tssH"
                     /locus_tag="ACHXOL_001646"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFR6808652.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESEQDNTGAEPADEADREQPEETAETVSPVQRL
                     AQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGD
                     TIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGR
                     QYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHP
                     DVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPV
                     LADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGVEVVIEPEVTDE
                     IMSRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVE
                     DAQNDESVTKDETVL"
     gene            12581..13324
                     /gene="vasI"
                     /locus_tag="ACHXOL_001647"
     CDS             12581..13324
                     /gene="vasI"
                     /locus_tag="ACHXOL_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR6808653.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13329..14741
                     /gene="tssA"
                     /locus_tag="ACHXOL_001648"
     CDS             13329..14741
                     /gene="tssA"
                     /locus_tag="ACHXOL_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR6808654.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGQLAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14850..18284
                     /gene="tssM"
                     /locus_tag="ACHXOL_001649"
                     /pseudo
     CDS             14850..18284
                     /gene="tssM"
                     /locus_tag="ACHXOL_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            18295..>19639
                     /locus_tag="ACHXOL_001650"
     CDS             18295..>19639
                     /locus_tag="ACHXOL_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFR6808655.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTA"
BASE COUNT         4567 a         5430 c         5474 g         4168 t
ORIGIN      
        1 tatttaaatt attgccataa tacacctgac gcgaattgca caggttcaca caatctattt
       61 aacgcttgca gaatacccct ctttacgatc gataaaaagt ccttttctta tttattttga
      121 agcgtaaaat gcggggtgat gaaggagtgg tgattttatt ttcaccgcca ttagggcggc
      181 attgccgata tttttttgcg gtacaaatat gccgtgttca tggcctgtac gagataacat
      241 gacggatcac ttttatgagc aaaaaatttg aaggcagcgt cgcgccccgc gagcgtatta
      301 atatatcgta tgtcccaaaa acagatggcc agacggctga agtggaactt ccgcttaaca
      361 tgctcgttgt cggcgatacc ggaaatacgc aggaaacatc cccactggat gaacgtcagg
      421 cagtttccgt taacaagcac aatttcggtg cagtgatggc tgaagcagcc attggcctca
      481 attttactgt ccccgccacg ctgaagggaa gcacaacaga cgatgaactg aatgtggcgc
      541 tgaatatcaa atcactggat gatttttcac cagatagcgt ggcccggcag gttccggaag
      601 tgaataaatt gctggaactg cgtgaagcac tgactgcact gaaggggccg atgggtaacc
      661 ttccggcatt ccgtacccag ctccaggcat tgctggaaaa tgaagagtcc cgcgagcagt
      721 tgctcaaaga aatcggtctg gtgagcaata aataaccacc actatttcat taaacagggt
      781 tatccaggca tgacggcaca caaaaatatc tccgggacat accacctctc tgttgcagac
      841 attctgcagg tggtttacca ggtatgtttc agcccgtccg ttgaaatcaa ccaagatggt
      901 gtggctgcgc tgattacaac actggatcgc cgtatcagtg atctattgga tgagattatc
      961 catttttgtg agtttcagca gagcgcgtcg cactggcagc gcgtacttca ctgatggcga
     1021 aaagccggtt tcagaaacac agctaaaaca gaaggatttt cagtatgtct ttacaggaag
     1081 aggaactcgt ttccagtcat gccgggcagc ctgagcaggc atcgtcgctg cttgatcaaa
     1141 tcatggcaca gacccgtatt cagcccggtt cggaaggtta tgacgttgcc ctccagggtg
     1201 tgacagcctt tattgccagc atcctgcaat ccactgcatc tgccgaaccg gtcaataaac
     1261 tggccgtgga cagcatgatt gcagatattg atgaacgcat cagccgccag atggatgtca
     1321 tcatccatgc gcctgccttt cagcaggtcg agtcgttctg gcgctcactg aaaaccatgg
     1381 tggatcgcgt tgatttccgt gaaaacatca aggtcaacgt gcttcatgtc accaagcagg
     1441 aacttctgga agactttgag tttgcgccag aaattattca gtccggattt tataagcacg
     1501 tttattcgtc cggtttcgga cagtttggcg gtaaacccat cgctgccgtg ctgggcgctt
     1561 atgagttcaa aaataccgcg cctgacatga aactgctgca atacgtcagt gccgtgggcg
     1621 caatggcgca cgcaccgttc ctgtcctccg tttccccgga gtttatgggg ctgaactcgt
     1681 ggaccgaact gcctaatatc aaagatcttt atgccatctt tgaaggtccg gcctacacca
     1741 aatggcgcgc cctgcgtgac tcggaagatt cgcgctattt agggcttacg gcaccccgct
     1801 tcctgcttcg ccagccctat tcaccaacag ataaccctgt taagaacttt aactattacg
     1861 aagatgtcag ccagaaccac gaagattatc tgtggggtaa tacggcctgg atgctggcat
     1921 gcaatatcgc agacagtttt gccaaatacc gctgatgtcc aaacattatc ggtccgcaaa
     1981 gcggcggcgc agtgaaagat ctgccgatgc atctgttcga aacgatgggg cagattcagg
     2041 ccaaaatccc aaccgaggtg ctggtcaccg atcgccgcga atttgaactg gctgaagagg
     2101 gttttatcac ccttaccatg cgtaaagact ctgataatgc agcctttttc tctgcaaact
     2161 cggtacaaaa accgaagcac tttccgggaa aagatgcaga aaccaactat aagctgggta
     2221 cgcagcttcc ttatctcttc atcatcaatc ggttagcgca ctacatcaag gtgttgcagc
     2281 gtgaacaact ggggtcatgg aaagaaagga gcgacttaga aagagagctt aatacctgga
     2341 tccgtcagta tgttgccgac caggaaaacc cgcctgcaga cgtgcgcagc cgcaaacctc
     2401 tgcgcgctgc caaagttgag gttatggatg tagaaggcga acccggctgg taccaggttg
     2461 cgctaagcgt gagacctcat tttaaattta tgggggcaaa ttttgagctt tccctggttg
     2521 gccggttaga cagggagtaa cacaccatga taccggatcg ggaaagaacc acaggttctc
     2581 tttttgagcg tatggaagcc tcttcggcca gaaaccggca gggaggcagt attcattcgc
     2641 tcaggcaatc cattcgccag aacctacgaa acattcttaa tacccggtcc ggtagttgcc
     2701 ggggtgcgcc tgagctgggc attgatgaac cggaaggggc tgaaaacttc cgcgaatcca
     2761 tgagtcgggc catcgagcaa tgtatagaac gctatgaacc ccggatctca cacgctgaag
     2821 tgcaggcagt ggtttcatcc gcctccagcc cgctggatat gacgtttcat attacagcct
     2881 gggtgacatt taacgaaacg catgaggtgc ttgaatttga tatggcaccg aatggcagcc
     2941 agcattaccg ggtggactga caaatggaat ttgaagaacg ctatttccgg gaagaactcg
     3001 attacctgcg ccagcttagc aagctgctgg caacggaaaa accccatctg gcccgcttcc
     3061 tggccgaaaa agatgcagat ccggatattg aacgcctgct ggaaggggtg gcttttctta
     3121 ccggcaatct ccgccagaaa attgaggatg aattcccaga actgacgcac gggcttatta
     3181 agatgctatg gcctaattac ctgcgtccgg ttccggcaat gacccttatt gaatatacgc
     3241 cggatatgga taagtcttct gtaccggtgt taatcccccg taatgagcag tttacaacca
     3301 acgccgggga aatcagagtt gatgaagtgc tgccctctga tgctaaaaag gaggagccgc
     3361 ctccctgtac cttcaccctc tgccgggata tctggctgct gcccgttcgc ctggagcaga
     3421 ttgaaaaccg cagtacgacc cgtaatggtg ttatcaacat caccttttcg gtcgcaccgg
     3481 gaacggactt ccgcacgctg gatctgaaca aacttcgctt ctggctcggc aatgacgaca
     3541 actatacccg tgaccagctt tatttatggt tctgcgaata cttgcagggt gccgacctga
     3601 ctgtgggtga acagcatatt cgcctgcctg agtttatgct aaaagctgtc ggttttgagc
     3661 cgcaggatgc catgctgccc tggccgaaaa acgtccacag cggctaccgg atccttcagg
     3721 agtatttctg ttaccccgat gcgtttctct tttttgatct ttgtggttgt ccggctttgc
     3781 ctgacggatt gcaggcggaa ttctttaccc tgcaactgcg tttttcgcgc cctttgcccg
     3841 tggacatccg gctgcgccgc gattccctgc gcctgtattg cgcacctgcc attaatttat
     3901 ttatccacca tgcagaagcc atcacgctgg acaaccggcg ggcagactat ccgctggttc
     3961 ccagccgcca ttacccacaa cattacgatg tattttccgt taacagtgtg gtgagccagg
     4021 tccaggatat gttcaggaaa aaagatctgg ggcgtcctgt ttcgacgcag gccgcgcgcc
     4081 agtggccagc ctttgaaagt ttcagccatc agatagaata cagccggaag cgggaagtgg
     4141 tgtactggca tcaccggacc aaaacatccc tgttccatcg cggctttgat catacccttg
     4201 cctttataca tgctgatggc agttatccgt cagacgaatc tctgctcagt aatgaagtgg
     4261 tttcggtatc gctgacctgt accaaccgtg agcttccgtc acaaattcgt tccggcgata
     4321 tcaccggcac aaccggaaaa aatgcggctg ttgcttcatt tcgcaacatt acccgcccga
     4381 cgcaaccact ctggccggtc attgatggca gcctgcactg gcccctactc tccgccatga
     4441 acctgaatta tctgtcatta ctggatacgg acgcgctgaa gcaggtcatc gccaactttg
     4501 atcgccacgc aatccatcat ccgcagacgg cacggctgtc acaacaaaag ctggatgcca
     4561 ttgagcgtct ggagacccgc cccgttgatc gcctgtttac gggtattccc gtccgggggc
     4621 tggcttccac gctgtatctg cacccggagc cgtttgtctg tgaaggggaa atgtatctgc
     4681 tcggtacggt gctttcgcat tttctgtcgc tgtacgccag cgttaactca ttccacatgc
     4741 tgaccgttgt gaacacagaa agccaggaga catggaaatg gacggaaaga atcgggcagc
     4801 atcctcttat ctgagtccgg gaaatccccc ggcagataaa gagcagaatg atccgctggc
     4861 acaggttttc cataatgcct gctcttacaa tttttttgcg atggcggagc tgttgcaccg
     4921 cctggcaaag ggtgaaaagg ggacgccaga attatccctg cgcgacgatc cggcacagga
     4981 aaccctgcgt ttcagcgccg atgccagcct tgccttcccg tgcagtgata tcagtgcgct
     5041 gaaacgggat acgtcagggg cattcaggat gaccaccacc tttatggggt tacaggggag
     5101 ccagtcgcct ctccccggtt attacctcgc tcacctggcc tggaaagcgg tacatgaaca
     5161 aagtccggta ggtgattttc tggatatgtt cagccaccgc ctgacccagt ttgtctggca
     5221 tatctggcgt aagtaccgct atcacatcag ttttcgtaac ggcggtgtgg atgcgttctc
     5281 gcagcgcatg tactcccttg tcgggctggg ccaccgccag cttcgcgata agctggcgat
     5341 taatcacagc aaaatgctgg cgtattccgg gatcctggca aatccgggac gatccccgga
     5401 aattatctgt ggcctggtgt cccactgctt tgatttgtca gaagtgacac tgcaaaactg
     5461 gcaacgtcgc aaggtggata ttgaaccgga tcagcaaaat agtctgggga gctattctct
     5521 taaaaatggc gaaaaactgg cgggaagatc ggtactgggc aactttgttc tgggtacacg
     5581 agtgcccgat ctcagcggaa aattccagct cagtattacc agcctgaccc gaaaacagtt
     5641 cctctctttt ctgccgtccg gcgaaaactt cctgccactg acgatgttcg tgtccttcat
     5701 cctgcgcgat cagcttgcct gggacttaca tctggggctg gcgccggagc aggtgggcgc
     5761 aatgcgcctg ggcgataaca aaagtgcgct gctgggctgg accagcttcc tcggcacacc
     5821 ggaagaacga ccctcagtca ccatcagggt acggtcgtaa attatgataa tcacggagca
     5881 gaatatgccg gaagagaaac tacagacgct ttcattgcag gtcattaacg gcagtgagct
     5941 ggaaagcggg cgggcggcgc gctgtctgtt cacacagcag ggaaatgtcg gccatggccc
     6001 cgaatgccac tggtcggtac aggatcgtca gcagagcatt ccggcccagg cttttaccgt
     6061 tatcctgcac gatggcacat tttgtctgcg cccgcagacg gcacaactgt ggctgaatca
     6121 ggcaaaagtc acagcaacat cagacctaat acagttgcgc cagggcgatg agatccagat
     6181 cggacggctg atggtgaggg ttcatctgaa ccggggagat attccccatt acgatgagga
     6241 aatggccact cccgaaacca tcgttaccaa tcgcgatatg ctcacggata ccctgctatc
     6301 aacggagggt gcgccacact atccgggaat gactcaccgg caccagcttg cagacaccgt
     6361 ggtaaacggt ttttctgccg atccactcca ggctcttcag tccgaaagcc tgattaccac
     6421 gggcgatccg ctttcaggca ttgcggctgt ccggccatcg gcaccgctgt ccgatccggc
     6481 aagtaatggg gggatcaata ctccgtttat ggatctgccg cccatttatg ccagccccgg
     6541 cgatcgtaat gacgacgtct ctgcggcaga aatggcgcaa cgccacgttg cggtcacccc
     6601 cttactgcgc ggtcttggcg gctcgcttac catgagcaat tccgacgatg cggatgattt
     6661 tctggaggag gccggacgaa cgttacaggc cgcaataaaa ggtctgctcg atttgcagca
     6721 gcagcgtaac agcctctcag acaaacattt gcgcccgctg gaagataacc cgctgcgcct
     6781 gaacatggat tacgccaccg cgctcgacgt aatgtttgcc gaaggtaaaa gcccggtaca
     6841 tctggcggct cccgccgccg tcagtgaaag cctgcgcaat gtccgccacc acgaagaagc
     6901 taaccgggca gcgattgtgg agtcgcttcg tgtcctgctg gatgctttct caccacaaaa
     6961 tctgctgcgc cgctttgtgc agtaccgccg cagccatgaa ctgcgccagc cgctggatga
     7021 tgccggagca tggcaaatgt acagccatta ttacgaagaa ctggcctccg atcgccagca
     7081 ggggtttgag atgctgttta acgaggtcta cgcccaggtc tatgaccggg tgcttcgtga
     7141 aaaacagcgg gagccggaag catgaacagg aaggcgtttc tggcctgcgt gctgatgtgc
     7201 gtcatattaa ccggctgcga aacggcgaaa aaaatcagtg aggtgatcaa aaacccggat
     7261 atccaggtcg ggagcctgaa ggagcaacct tcggaaataa ccgtcaccct gctgaccgaa
     7321 ccggacacca ataccaatgc ggaaggtgaa tcagccgccg tggatgtgca actggtgtac
     7381 ctgactgacg actcaaaatt gctggcagcc gactatgacc agatagccag cacgccgcta
     7441 ccggatgtgc tgggtaaaaa ctatatcgac caccaggatt tcaacctgtt gccggacacc
     7501 atcaaaaccc tgccgccggt aaagctggat gagaaaacgc agtttattgg tgtcgtggcc
     7561 tatttctccg atgaccaggc cactgaatgg aaacaaatcg agacggtgga aggaatcggc
     7621 caccattacc gcctgctggt gcatgtacgt caaagcagca tcgaaatgaa aaaagaggat
     7681 gaataattat ggcaaccacc cgcaacaagg tgatgtggca ggaagggatg ctgatgcgtc
     7741 cacaccattt ccagcagcag cagcgttaca acgactacct ggataaccag cgtttccggg
     7801 ccatgaatga tttatcctgg ggatttaccg aactcaccct caacaatgaa ctgctggcgc
     7861 agggtaagat catgattgac agcgcgtcag gcacactgcc cgacggcacc gtcttttcta
     7921 tccccgacca ggacgcactg cccgatccgc tgcacccgca aaattttccg gacgaaagaa
     7981 gccgcaatat ctacctcgct ctgccggtcg ccagtgatgt gagaaatgaa atcagcgacg
     8041 gacggcgaat cgggcgttac cggctgaatt atgccgatgt ccgggatttg cattcagaag
     8101 aaggtgacgc gcgaacattg acgctgggtc aactgacgcc gcgcattatg agcggtgcag
     8161 aagatatgag cgcctatatt acgctcccgc tttgccgtat cagcgatcgc catgccgatg
     8221 gctccctgac gttggatgac gattttatcc cctcctgcca gaatattcag gtcagtaaga
     8281 aactgcgtgt ttatctcaaa gaggtacagg gggccattgg cggacgggca agcgatctgg
     8341 caaaccgtat tggctctccg gcgcagagcg gcatcgcgga tgtggcggaa tttatgatgt
     8401 tgcagttact taaccgtaac cagacccggt ttacccatca cgcccgtcga tcccagctcc
     8461 acccggaaga tttctacctt gatcttgccg ggttgctggg tgaactgatg acctttaccg
     8521 agccgtcgcg cctgccctgc ccgcttgatg tgtatgatca tcatgacctg accaaaatat
     8581 ttaaaacgct gttaccggaa gtcaaacggg cgctgcatac cgtactgtcg ccaagagccg
     8641 tcaatctgcc gctgcatctg cgtgacggta tctggcaggc cgatatccat gacacagaac
     8701 tgctgcaatc tgccaccttt gtgctggccg tggcggcaaa tatgccggtc gatcagatcc
     8761 agcgccagtt tatccagcag tcgaaaattt cctcgccgga aaaaatccgc aatatggtca
     8821 gtgtgcagat cccctgtatt ccattgcgtg cccttatggt ggctccccgc cagcttcctt
     8881 accattccgg gttcagctat ttcgaactcg acaagagtgg ccaggcctgg acagaaatgg
     8941 ctgccgccgg ggccgttgca ctgcatgttt ccggcagttt cccggatctg aacatgcaac
     9001 tgtgggcgat aagagggtaa gacgatggat gaggggtcac tgtctctgcc accatttacc
     9061 ggacacgacg aaaaaagcca gcgcaactac catctcgcgc ttcgtggcaa cagcctgaat
     9121 ccgatgatcg acgcggccac gccgctactc ggtatggtaa tgcgcctttc aaccatgaac
     9181 agccagacga tgccggagca tctttttgct caggtggtga cagatgttca ggccgtggag
     9241 caactgcttc aggagcaggg ctacgaaccg ggcgtgatta tctcgtttcg ctacatcctc
     9301 tgcacgttta ttgatgaagc ggcgctcggt aacggctggt caaacaagaa cgagtggatc
     9361 aagcagtcat tgctggtcca tttccacaac gaagcctggg gtggcgagaa agttttcatc
     9421 ctgctggagc gcctgatccg tgaacccaaa cgttaccagg atctgctgga atttctctgg
     9481 atctgttttt ctctgggatt tcgcgggcgc tacaaagtgg cagcacagga tcagggggaa
     9541 tttgagcaga tctaccgccg tctgtaccac gttctgcata aactgcgcgg cgatgcccct
     9601 ttcccgctgt tacaccagga caaaaaaacc cagggcgggc gttatcagct catcagtcgc
     9661 ctgactgtta aacatatttt ttgtggtggc gttgtcgtgc tggcgttgtt ttacctgttc
     9721 tatctgttgc ggctggacag ccagacgcag gacatcctgc accagctaaa caaattactt
     9781 cgataaggac gtttatgatc cagattgatc ttcccacgct ggtaaaacgg ctgaacctgt
     9841 tctcccgcca ggcgctggag atggccgcct ctgaatgtat gagtcagcag gcagcggaaa
     9901 ttaccgtcag ccatgtgctc attcagatgc tcgccatgcc acgcagtgac ctgcgggtta
     9961 ttacccgcca gggcgatatt ggcatggaag agttgcgcca ggcgctgacg gtagagaact
    10021 acacaaccgc ccgttctgcg gacagctacc cggcgttttc cccgatgctg gttgagtggc
    10081 ttaaagaggg ctggctgctg gcgtcggctg agatgcagca cagcgaactg cgcggcggcg
    10141 tgttgctgct ggccctgctg cattcgccgc tgcgttatat accgcctgct gccgcccggc
    10201 tgttgaccgg cattaaccgt gaccgtctgc aacaggactt tgtgcagtgg acacaggagt
    10261 cggcggaatc agtcgtgccg gatgcagacg gtaaaggcgc aggcacaatg acggacgcct
    10321 ctgacaccct gcttgcccgc tatgccaaaa acatgaccgc agacgcccgt aacggcaggc
    10381 ttgacccggt actgtgccgc gaccacgaaa tcgacctgat gatcgacatt ctctgccgcc
    10441 gccgtaaaaa caacccggtg gtggtgggcg aagcgggcgt gggcaaaagc gcactgattg
    10501 aagggctggc gctgcgcatc gtggcaggcc aggtgccgga caagctgaaa aacaccgata
    10561 tcatgaccct tgacttgggc gcattgcagg ccggggcgtc ggtgaaaggt gaattcgaaa
    10621 aacgtttcaa agggctgatg gcggaggtca tttcctcccc ggtgccggtc attctgttta
    10681 tcgacgaagc acataccctg attggcgcgg gcaaccagca gggcgggctg gatatctcca
    10741 acctgctcaa accggcgctg gcgcgcggcg agctgaaaac catcgccgcc accacctgga
    10801 gcgagtacaa aaaatacttc gaaaaagatg ccgccctgtc gcgccgcttc cagttggtga
    10861 aggtcagcga acccaacgct gccgaagcca ccattattct gcgcggtctg tcggcggtct
    10921 atgaacagtc tcacggcgtg ctgattgatg atgacgcctt gcaggccgct gcgacattaa
    10981 gcgagcgtta tctctccggg cgtcagttac cggacaaagc gattgatgtg ctggataccg
    11041 cctgcgcccg tgtggccatc aacctgtcgt cgccgccgaa gcaaatctcg gcgctgacca
    11101 ctctgagcca ccagcaggag gcggaaattc gccagcttga gcgcgagctt cgcatcggac
    11161 tgcgtaccga cacatcacgg atgaccgagg tgctggtgca gtatgatgaa acgctgacgg
    11221 cgctggatga actggaagcg gcctggcacc agcagcagac gctggtccgg gagattatcg
    11281 cgctgcgcca gcagttactg ggcgtggcag aggacgatgc ggcgccgttg ccggacgcag
    11341 ataccgtgga ggatacgcag ccagagtcag agtcagagtc agagtcagag tcagagtcag
    11401 agcaggataa taccggtgcc gaaccggctg atgaggccga cagagaacaa ccggaagaga
    11461 ccgctgaaac agtttccccg gtacagcggc tggcccagct cactgccgaa ctggacgccc
    11521 tgcataacga ccggttgctg gtctccccgc acgtcgataa aaaacagatt gcggcggtga
    11581 ttgccgaatg gaccggcgtg ccgctcaacc gcctgtcgca gaatgaaatg tcggtcatca
    11641 ccgacctgac gaaatggctg ggtgacacca tcaaaggcca ggacctggcg attgccagcc
    11701 tgcataagca tctactgacc gcacgcgccg acctgcgtcg tccgggacgc ccgctcggtg
    11761 cgttcctgct ggctggcccc agcggtgtgg gtaaaaccga aaccgtcctg caactggcag
    11821 agctgctcta cggcggtcgc cagtacctga ccaccatcaa tatgtccgaa ttccaggaga
    11881 aacacaccgt ctcgcggctg attggttcgc ctccgggcta cgttggctac ggtgaaggcg
    11941 gcgtactgac cgaagcgatt cgccagaaac cctactcggt agtactgctc gatgaagtgg
    12001 aaaaagcgca cccggatgtg ctcaacctgt tctaccaggc gttcgataag ggcgaaatgg
    12061 cagacggtga aggccgcctg attgactgca aaaatatcgt cttcttcctg acgtccaacc
    12121 tcggctacca ggtaatagtc gagcatgccg atgacccgga aaccatgcag gaagtactgt
    12181 atccggtgct ggccgacttc ttcaaacctg ccctgctggc gcgtatggaa gtggtgccgt
    12241 atctgccgct gtcgaaagag acgctcgcca ccattatcgc cggaaaactg gcccgtctgg
    12301 ataacgtgct gcgcagtcgc tttggtgtag aagtggtcat tgaaccggaa gtgacggacg
    12361 aaatcatgag ccgcgtcacc cgcgcggaaa acggcgcgag gatgctggaa tcggtcatcg
    12421 atggcgacat gctaccgccg ctctcgctgc tgctgttgca gaaaatggcg gctaacacgg
    12481 cgattgcccg gattcggttg tcggcagtgg acggcgcatt tacggcagac gtggaagatg
    12541 ctcagaacga cgagtccgtc acaaaggatg aaacggtttt atgagcaggt tgcgcgtttt
    12601 ccggcagaca ggatggttta tcgcgggcct gatgacgggc ttacccgcca ctgccgcgcc
    12661 cgcagaagca tcgtcaatgg ccggtgtggc ggtcgccgtc gccaccacga ccccgccgga
    12721 tgcgacagcc acgttacagg ccatgcagtc ctgtcggcgg gagtccgcgg cgctggagcg
    12781 gcttgactgc tatgaccgcc tgctggcacc gctgtccccg tcagggtttg acggggcgct
    12841 ggtcaaagcc ggttttgtgg gcgaggcctg gacccgcgcc acagagcagg aaaaacgccg
    12901 cgagggcaac accacagaac tgctggtgac gcaggtgccg ggggagcgcc cgacggtggt
    12961 gataaccacc ccggctatcg gtcatgttcc gccacgcccg gtgctgatgt tcagttgcgt
    13021 ggacaatatc acccggatgc aggtggcgct gatgcaccct ctggatgtcc atgatatcgc
    13081 cgtcactctg aatgccgaca accgggcatt gcgcagccac tggtttgtgc gcgagaacgg
    13141 caccctgctg gagtccagcc gggggttatc cgggattgat gaaattaagc aactgttcgg
    13201 cgcaaaaacg ctgacggtgg ataccggcgc agacaatgcc gccggaaagc tgacctttaa
    13261 cattgacggg ctggcaaggg cgattgctcc cctgcgcgat gcctgccact gggcgggaga
    13321 ataaaaatat ggcaatggac ttacgcgatc cgaatgtctg gatatcgcac ctgctggaaa
    13381 acctgccgga agagaaactg gcatcggcgc tgaaagatga caacccgaac tgggagtata
    13441 tcgacggcga aatcgtcaag ctgggatcgc tggcccacag tcaactggat atcccggaac
    13501 tccagcgccg ggggctggtc attctggctt cggagagtaa ggattttcgt ctgctggcgc
    13561 atctgctaag aaccctgcaa cacgccggtg atcctcatct cgcattgcgc ctgctggcgc
    13621 tgtatgtgga gcattactgg gcggtggctg cgccgcagaa tatggcgcat aaaaaacgct
    13681 ttgccagcca ggtcattaaa cgctttgaaa caggtattga agttttttca cagaacgccg
    13741 ccacagcgca gcgcgatgcg ctgtcagggc aactggcaaa actggcgcaa tgctggcagt
    13801 cacataacgc cccggaactg gcacaggcta ctgatgatct ttttgccctg taccagcgtg
    13861 cgtttaatcg tgcggcaccg gctcctgtcc ccactccggc ggcctccggt agttcaccac
    13921 agaccaccgc cacgtctgaa agcggcgtga cgcaacccag tgctccggct ccccaaatcg
    13981 tcatcgacag tcacgacgac aaagcctggc gcgacacgct gttaaaagtg gcggccattt
    14041 tatgtgaacg ccagccggat tcaccgcagg gctaccgtct gcgccgccat gcgctttggc
    14101 agaacatcac cagtacgccc caggcggaaa gcgatggccg tactccactg gctgcggtct
    14161 ccgccgatat ggtggcagat taccatgctc agctcggtag tgccgatatg gcgctgtggc
    14221 agcaggtgga gaaaagcgta ctgctggctc cttactggct ggatggtcac tgcctttctg
    14281 cacagacggc gctgcgtctg ggttacaaac aggtggcaga cgccatccgc gatgaggtca
    14341 tccgcttcct tgagcgtctg ccccagctta ccgggctgct gtttaatgac cacacaccgt
    14401 ttatcagtga gcagacgaaa caatggctgg cagcttcgcc cgacgccaaa gttgcaccgg
    14461 ttgcgcaaat cggtgaggaa tcgaaggcag ccagagcctg ttttgctgag cagggtctgg
    14521 aggcggcgct gcgatatctg gacatgctac ccgaaggcga tccccgcgat cagtttcacc
    14581 gccagtacct tgccgcacag ttgacggaag aggcggggct ggtacagctt gcgcagcagc
    14641 agtaccggat gttgttcagg atgggattgc agatgatggt ggcagactgg gagccgtccc
    14701 tgcttgagca gctagaacaa aaattcacgg cagaacaata aaagagaaca ggagtttttg
    14761 tgttcagatt accaacaccc cgactactca gcggactcaa atcagccctg cgaccggcga
    14821 tgccccggtt taaagtctct gctttctggc tgctgatact ggcgtggatt tttctgctgg
    14881 tgtggatctg gtggaaaggc ccgatgtgga cgctgtatga agaacagtgg ctcaaaccac
    14941 tggcgaaccg ctggctggca acggcggcgt gggggattat tgccctggtg tggcttaccg
    15001 tccgggtgat gaagcgcctg caacagcttg aaaaaatgca aaagcaacag cgcgaggaag
    15061 ccgttgatcc gctcagcgtg gaactgaacg cccagcagcg ttatcttgac cgctggctgc
    15121 tgcgcctgca acgccatctc gacaaccgcc gtttcctgtg gcagttgccg tggtatatgg
    15181 tcatcggccc ggcgggcagt ggcaaaacga cactgctgcg caaggggttt ccgtccgaca
    15241 ttatttatgc cccggagggc gcacgcggcg cagaacaacg cctgtacctc acgccccatg
    15301 tcggtaaaca ggcggtgatc tttgatatcg acggcacact ctgcgctccc gctgatgcgg
    15361 atatcctgca tcgccgtctg tgggaacatg ccctcggctg gctgaaagaa aagcgcgcgc
    15421 gccagccgct gaacgggatt attctgacac tcgatttacc cgatctgctg accgcagaca
    15481 aacgccgccg cgagcatctg ttacagacgc tgcgcagccg cttacaggat atacgccagc
    15541 atcttcactg ccagttaccg gtttacgtgg tactgacccg gcttgattta ttacagggtt
    15601 ttgccgccct gttccagtca ctcaacagac aggatcgcga tgcgattctg ggcgtcacgt
    15661 tcacccgccg tgcccatgaa aatgatgact ggcgaacaga gttgaatgct ttctggcaga
    15721 catgggtgga tcgaatgaat ctggcgttgc cggatctgat ggtcgctcag actcacaccc
    15781 gcgcgtcttt attcagtttt tcccgccaga tgcagggaag ccgtgaaccg ctggtgtcac
    15841 tgcttgaggg tctgcttgat ggcgaaaata tgaacgtgat gctgcgtggt gtctatctca
    15901 cctcttcgct tcagcgtgga cagatggatg atatattcac ccagtctgcc gcccgccagt
    15961 accggctggg caataaccca ctggcgtcct ggcccctggt ggacaccgcg ccttatttca
    16021 cccgcagcct gttcccgcag gcattactcg cagagcctaa tctggcaaca gagagccgcg
    16081 cctggctgat acgttcccgt cgccgcctga cggttttctc tgccacaggc ggcgtggcag
    16141 cactgctgct catcaccggc tggcatcact attacaacgg taactatcag tccggcatca
    16201 ccgtgcttaa gcaggccaaa gcctttatgg acgtgccgcc tccgcagggg gaagatgact
    16261 ttggcaacct gcaactgccg ctcctgaacc cggtacgcga tgccacactg gcctatggcg
    16321 actggggcga ccgcagccgt ctggccgata tgggactgta ccagggacga cgtatcgggc
    16381 cttatgtgga acagacctat ctgcaactgc tggagcaacg ttacctgccc tcgctgttta
    16441 acgggctggt caaagcgatg aacgccgcgc cgccggagag tgaagaaaaa ctcgcggtgc
    16501 tgcgcgtgat gcgaatgctg gaggacaaaa gcggacgtaa caatcaggtg gtgaagcagt
    16561 atatggcaaa acgctggagc gaaaagttcc acggtcagcg cgatatccag gcacaactga
    16621 tgtcccatct tgactacgcg ctggctcata ctgactggca cgcagagcgt caggcgggcg
    16681 acggtgacgc catcagccgc tggacgccat atgacaagcc cgtggtatca gcacagaaag
    16741 aactgagcaa actgcctgtc taccagcggg tttaccagag cctgaaaacg cgggcgctgg
    16801 gcgttcttcc tgccgacctc aatctgcgtg accaggtagg gccaaccttt gaccaggtgt
    16861 ttacatctgc cgatgacaac aaactggttg ttccacagtt tatcacccgc tacggcctgc
    16921 aaagctattt tgtaaaacag cgcgatgaac tggttgaact gacagcgatg gattcctggg
    16981 tacttaacct cacccgcaat gtgaaataca gtgacgccga ccgtgcggaa atccagcacc
    17041 agttgaccga gcagtatatc agcgactaca ccgccacctg gcgggctggg atggacaatc
    17101 tgaatatccg caattttgag tccatcggac aactgaccgg ggcgctggag caggttatca
    17161 gcggcgacca gcctttgcag cgggcgctga ccgtgctgcg tgacagcaca cagccaggcg
    17221 tcttttctga aaaactctct gccaaagaac gggaggaagc cctggcagag ccggattacc
    17281 agttactcac ccgcctcggg catgaattcg ccccggaaaa cagtaccctg gcagtacaga
    17341 aagacaaaga aagcacgatg caggccgtgt atcagcaact caccgagttg caccgctacc
    17401 tgctggcaat ccagaacgcg cctgtaccag ggaaatcggc gctgaaagcc gtgcagttac
    17461 ggcttgatca gaacagcagc gatccgatat tcgccacccg ccagatggca aaaacgctgc
    17521 ctgctccgct caaccgctgg gttggcagac tgactgacta ggcctggcat gtggtgatgg
    17581 tggaggctgt tcattatatg gaagtggact ggcgcgacag cgtggtgaaa ccgtttaacg
    17641 agcaactggc aaataactat ccgtttaatc cgcgttctgc acaggatgcc tcactggatg
    17701 ccttcgaacg cttctttaaa ccggatggca tactggatac cttctaccag cagaacctga
    17761 agctgtttat cgataatgac ctgagtctgg aggatggcga taacaacgtc attattcgcg
    17821 aagatattat tgcgcaactg gaaactgcgc agaaaatccg tgacatcttc ttcagcaaac
    17881 agaacggtct gggaacatcc tttgccgtgg aaacggtatc gctttcaggc aataaacgcc
    17941 gcagtgtact gaaccttgac agtcagttag tcgattacag ccagggccgt aactataccg
    18001 cccatctggt ctggcctaac aacatgcgcg aaggcaacga aagtaagctg acgctcatcg
    18061 gcaccagcgg caacgcgccg cgcagtatca gcttcagcgg gccgtgggcg cagttccgcc
    18121 tgttctgggc cggacaactg accggagtac aggatggcaa ctttaccgtg cgctttagcg
    18181 tggacggtgg cgcgatgacc taccgtgtgc ataccgacac ggaagataac ccgttcagcg
    18241 gtgggttgtt cagccagttt ggtctgtcag acacactgta ctgaggaggc agggatgaat
    18301 agtaacgtac tgacacaaac tatcgttacc ggcagtgacc cgcgcgggct gccggaattc
    18361 agcgccatcc gcgaggaaat aaacaaagcc agccacccgt cacagcctga gctgaactgg
    18421 aaactggtgg agtcgctggc gctggcgatt tttaaagcca acggtgtgga tttacacacc
    18481 gccacctact atacgcttgc ccggacacgg acacagggac tggcgggatt ctgcgaaggt
    18541 gcggaactgc tggcggcaat ggtaagccac gactgggata agttctggcc gcagggcggc
    18601 ccggcgcgta ctgaaatgct ggactggttt aactcccgaa ccggcaatat tctgcgtcag
    18661 caaatctcct ttgcggaatc cgacctacca ctgatctacc gcacagagcg ggcattgcag
    18721 cttatctgcg ataagctcca gcaggtggaa ctgaagcgcg ttccgcgcgt ggagaatctg
    18781 ctctatttta tgcagcacac gcgtaaacgg cttgaacccc agccgaagag taacgctgag
    18841 aacgccgcac agaccacggt cagaacgctg atttatgccc cggaaacact ggcatcttcc
    18901 acaccagaag cggtagtgcc tcccctgccc ggcctgcctg agatgaaagt ggaagtgcgc
    18961 agtctgacag agaatccccc acaggccagt gtgataaagc aaggcagtac ggtaagaggg
    19021 tttatcgcag ggatcgcctg ttcagtggct gtcgcctcag cattgtggtg gtggcaggtc
    19081 tatccggtgc agcagcaact gttacaggtt aacgacaccg ctcagggcgc agcaacggtg
    19141 tggatggcct cacctgaact cgaaaactat gagcgcaggc tgcaacaact tcttgatacc
    19201 tccccggtac agccgctgga aaccgggatg cagatgatgc gtgttgccga cagtcgctgg
    19261 ccggaaagcc tgcaacagca acaggcctcg acacaatgga atgaggcact caaaacccgc
    19321 gcacagagta gcccgcagtt gcgtggctgg ttgcagaccc gccaggactt acatgctttt
    19381 gcagatctag tgatgcagcg cgagaaagag ggactaaccc tttcctatat caaaaatgtc
    19441 atctggcagg cggagcgggg actggggcag gaaacccccg ttgagtctct gttgacgcag
    19501 taccatgatg cccgtgcgca gaagcagaat acagatgcgc tggaaaaaca aattaatgag
    19561 cgactcgaag gcgtgttaag ccgctggctg ctgctgaaga ataacgtcat gccagaggcg
    19621 gcaaccggta ccacggccg
//
LOCUS       DAZRUF010000058        19530 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZRUF010000058 DAZRUF010000000
VERSION     DAZRUF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.058"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHXOL_001651"
     CDS             <1..245
                     /locus_tag="ACHXOL_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR6808656.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHXOL_001652"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHXOL_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR6808657.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHXOL_001653"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHXOL_001653"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR6808658.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNNNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHXOL_001654"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHXOL_001654"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR6808659.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDDLDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHXOL_001655"
     CDS             3423..4061
                     /locus_tag="ACHXOL_001655"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR6808660.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHXOL_001656"
     CDS             4066..4842
                     /locus_tag="ACHXOL_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR6808661.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHXOL_001657"
     CDS             4931..6295
                     /locus_tag="ACHXOL_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR6808662.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHXOL_001658"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHXOL_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR6808663.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHXOL_001659"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHXOL_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR6808664.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHXOL_001660"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHXOL_001660"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR6808665.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHXOL_001661"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHXOL_001661"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR6808666.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHXOL_001662"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHXOL_001662"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR6808667.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHXOL_001663"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHXOL_001663"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR6808668.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHXOL_001664"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHXOL_001664"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR6808669.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHXOL_001665"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHXOL_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR6808670.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHXOL_001666"
     CDS             complement(12844..13167)
                     /locus_tag="ACHXOL_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR6808671.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHXOL_001667"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHXOL_001667"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR6808672.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHXOL_001668"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHXOL_001668"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR6808673.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHXOL_001669"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHXOL_001669"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR6808674.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHXOL_001670"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHXOL_001670"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR6808675.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   17469..17741
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17591..17619
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHXOL_001671"
     CDS             complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHXOL_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR6808676.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHXOL_001672"
                     /pseudo
     CDS             complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHXOL_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="ACHXOL_001673"
     CDS             complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="ACHXOL_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR6808677.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4606 a         5269 c         4895 g         4760 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acaacaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggcc cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgatct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgggca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca ggttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgatcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggca gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacacc ctgacgcgcc gcagtattta
    17641 tctgctctgg ccggtttatc cccgctggcg cggggaacac gggtgcgtgt ggctgccagt
    17701 gccggagaac ggcggtttat ccccgctggc gcggggaaca cactaagcat acataactgt
    17761 ttttaaacaa atttattccg catcaacaat ctaccaacta aattaaaaca tttccttatt
    17821 tttaaagaac ggataactta ttgattatca acaggaagaa aagaaaccaa acgtaaccca
    17881 tccaaatcca ccggaatacg tctgttttct ccccaggtct gaaattcaaa acccgactcg
    17941 atattggtcg cccaggccat caccacattt ccgcaaccag ccagttgggt aatttgctgc
    18001 cagatcatct cccgaatacg ttttgatgta tcaccaacat acacaccggc acgcacttcc
    18061 agtagccaga ttgcgagccg tccacgtaag cgcggaggga cattttctgt aacaaccacg
    18121 accatgctca tccgccgcgc ccccggtgac cactatcacc cagcgtttca ggttcaggga
    18181 tggcaggcgg taacatatcc ggcgcgggtt gtggtggttc aatttcacct gcagcaagga
    18241 cttcctcaat taacggtatt aatttgcccg ttaacttagt gctacggaaa atatcgcgac
    18301 aggctaatct gacttcttta tcaggttctg cgggttgcct cgctgctatt tcaaatgcct
    18361 ttggcacaac cgaatcaaat ttaatgatat cggctatgtc ataaacaaat gaaagcggtt
    18421 tgccactatg gataaaccca atagcgggcg catatcccgc ggctaatact gccgcttcag
    18481 atataccgta cagacatgat gtagcagcac tgatgcagcg attcacaaca tcgccttttt
    18541 cccagtcttt aggatcgtat ttgcgaccat tccatttcac accatattgt ttcgccagta
    18601 atgcatacgt ctggcgaacg cgagatccct caattccacg taactgctcc actgaacggc
    18661 gagctggcgg tggttcacga aaacgtaatt catacatttt gcgcaccacc ttcaaccgta
    18721 gatcttccgt taaagccagc tttgcctagt agagtaattt atctgcccgc gcccctccgg
    18781 gttgtccgga ggagtaaacg cgaacgcccg cttcaccgac ccagaccagc agtgttccca
    18841 ccgtggcggc cagatggact gccgcgtggg aaactcttgt tcccggttcg agcataatgc
    18901 aggcgaccga tcccactgga atatgcgtgc ggatcccggt tttgtcgatc agcacgaaag
    18961 cgccgtccag tacgtcgatt tgaccgtact ggaggaagat catggaagtg cgatctttta
    19021 atgggatcgg gctcagcggt acaaacgtca cgcctctgct ccgggtttga tcagcatcag
    19081 accacaaccg aacgcccggc ttttgccata cccctgactg agacgttgca gaaataaccc
    19141 aggagcggtg accgtaagca tccccgtata atccacgcta ctgaactgga tcagttgccg
    19201 ggagttttcc cgccgcagtt gctgttgtcg gtaggcatca accgaagtat ccagcagtgt
    19261 aaaaccgctt ctctccccct gcgctgccag ccagtccagc gcagcctgtt gttgatgcaa
    19321 ccagacatca cttccttcca cctgccccct cacctgccgt ttcgcctcca tcagcaagtc
    19381 gtggcgtttg cccgctttac aaattgttgg attcgcccgc aggttgaaac aaagggattg
    19441 tccggtacgc aactctggcg caaatggtcg gcattcgatg gtgaaaatat cactttccgc
    19501 cgggcgttcc tgcgacagga caaaaaagcg
//
LOCUS       DAZRUF010000059        19430 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZRUF010000059 DAZRUF010000000
VERSION     DAZRUF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.059"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..351)
                     /locus_tag="ACHXOL_001674"
     CDS             complement(<1..351)
                     /locus_tag="ACHXOL_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR6808678.1"
                     /translation="MTVKVIDLFCGAGGLTHGLQLAGLDVVAGIDLEGECRFPYERNN
                     KSKFIEQDIAKVTKEELLRLYGDASVKVLAGCAPCQPFSKYTQGKDKADDKKWPLLYE
                     FERLIREVSPEIVTM"
     gene            complement(438..2735)
                     /locus_tag="ACHXOL_001675"
     CDS             complement(438..2735)
                     /locus_tag="ACHXOL_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR6808679.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASMLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDSGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGLRRPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEVLARGATVNYDG
                     KKFTYPLADEWSGFSTVLESNSVKR"
     gene            complement(2725..3000)
                     /locus_tag="ACHXOL_001676"
     CDS             complement(2725..3000)
                     /locus_tag="ACHXOL_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR6808680.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(2997..3221)
                     /locus_tag="ACHXOL_001677"
     CDS             complement(2997..3221)
                     /locus_tag="ACHXOL_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFR6808681.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(3224..3523)
                     /locus_tag="ACHXOL_001678"
     CDS             complement(3224..3523)
                     /locus_tag="ACHXOL_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR6808682.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(3523..3747)
                     /locus_tag="ACHXOL_001679"
     CDS             complement(3523..3747)
                     /locus_tag="ACHXOL_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFR6808683.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3811..4311)
                     /locus_tag="ACHXOL_001680"
     CDS             complement(3811..4311)
                     /locus_tag="ACHXOL_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFR6808684.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4481..4753)
                     /locus_tag="ACHXOL_001681"
     CDS             complement(4481..4753)
                     /locus_tag="ACHXOL_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFR6808685.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            4890..5183
                     /locus_tag="ACHXOL_001682"
     CDS             4890..5183
                     /locus_tag="ACHXOL_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR6808686.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            5253..6233
                     /locus_tag="ACHXOL_001683"
     CDS             5253..6233
                     /locus_tag="ACHXOL_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR6808687.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTKVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(6419..6919)
                     /gene="cpxP"
                     /locus_tag="ACHXOL_001684"
     CDS             complement(6419..6919)
                     /gene="cpxP"
                     /locus_tag="ACHXOL_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR6808688.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7069..7767
                     /gene="cpxR"
                     /locus_tag="ACHXOL_001685"
     CDS             7069..7767
                     /gene="cpxR"
                     /locus_tag="ACHXOL_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR6808689.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7764..9137
                     /gene="cpxA"
                     /locus_tag="ACHXOL_001686"
     CDS             7764..9137
                     /gene="cpxA"
                     /locus_tag="ACHXOL_001686"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR6808690.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9243..9917)
                     /gene="yiiM"
                     /locus_tag="ACHXOL_001687"
     CDS             complement(9243..9917)
                     /gene="yiiM"
                     /locus_tag="ACHXOL_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR6808691.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10066..11049)
                     /gene="kdgT"
                     /locus_tag="ACHXOL_001688"
     CDS             complement(10066..11049)
                     /gene="kdgT"
                     /locus_tag="ACHXOL_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR6808692.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11309..11929)
                     /gene="sodA"
                     /locus_tag="ACHXOL_001689"
     CDS             complement(11309..11929)
                     /gene="sodA"
                     /locus_tag="ACHXOL_001689"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR6808693.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12214..13248
                     /gene="rhaT"
                     /locus_tag="ACHXOL_001690"
     CDS             12214..13248
                     /gene="rhaT"
                     /locus_tag="ACHXOL_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR6808694.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13245..14093)
                     /gene="rhaR"
                     /locus_tag="ACHXOL_001691"
     CDS             complement(13245..14093)
                     /gene="rhaR"
                     /locus_tag="ACHXOL_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR6808695.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(14167..15003)
                     /gene="rhaS"
                     /locus_tag="ACHXOL_001692"
     CDS             complement(14167..15003)
                     /gene="rhaS"
                     /locus_tag="ACHXOL_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR6808696.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15291..16760
                     /gene="rhaB"
                     /locus_tag="ACHXOL_001693"
     CDS             15291..16760
                     /gene="rhaB"
                     /locus_tag="ACHXOL_001693"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR6808697.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            16757..18016
                     /gene="rhaA"
                     /locus_tag="ACHXOL_001694"
     CDS             16757..18016
                     /gene="rhaA"
                     /locus_tag="ACHXOL_001694"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR6808698.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            18258..19082
                     /gene="rhaD"
                     /locus_tag="ACHXOL_001695"
     CDS             18258..19082
                     /gene="rhaD"
                     /locus_tag="ACHXOL_001695"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR6808699.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            19092..19406
                     /gene="rhaM"
                     /locus_tag="ACHXOL_001696"
     CDS             19092..19406
                     /gene="rhaM"
                     /locus_tag="ACHXOL_001696"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR6808700.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         4816 a         5037 c         4868 g         4709 t
ORIGIN      
        1 cattgttacg atttctggag atacttcgcg aattaaacgt tcgaactcat aaagaagagg
       61 ccatttttta tcatcagcct tatcttttcc ttgagtatat tttgaaaaag gttggcaagg
      121 tgcgcatcct gccaatactt ttacagacgc atcaccataa agcctcagca gttcctcttt
      181 tgttacttta gcgatatctt gctcaataaa cttagattta ttgtttcttt catagggaaa
      241 cctacattcc ccctcaaggt caatgcctgc aacgacatca agccccgcaa gctgcaaacc
      301 atgagttaat ccgccagcac cacaaaaaag gtctatcacc tttacagtca tcactcactc
      361 caaatctata cgatactcag tatacatccc tttctacaaa atctcgaggc ctaacttata
      421 aactcatatt tattgagtta acgcttcact gagtttgact ctaatactgt tgagaatccc
      481 gaccactcat cagcgagcgg gtaagtgaat tttttcccgt cataatttac ggttgcccca
      541 cgcgccagca cctcaagctc ccatcgctgc ggcctgatac cgttctgagc aaggtcaacg
      601 cggatacggg taatttgcat tcgttccgac cgggtcagtc tggccgatgg cgctatttca
      661 tgcggtttta acgggcttcc gtttctttgc tgacggtttg gtcttctcag tccgtgtttt
      721 aatgcacctc tgagcgccct cacaacctcc gggtcattcc attcgataac accgtcatct
      781 accagattaa gcactgctgc ggcgtgctca gaaggtgtgg gagccggtaa cgaagtatca
      841 ccaccagtga gctttccaca gttattgaca ggactccgag gcgcggcgat gccgcttttt
      901 aaagtcaaag gctcaacgac cggcactttc ggaacaatgc gccagtccgt cgttctggtg
      961 atatgaatat gacgcgcgcc gagatgcggc gcgtaaatgc cgaccactct ctcgacttct
     1021 tcctcgtact cgttaacttc atccgacgga ctacgggcga ccctgacagt ctgacaatcg
     1081 cgcgggacat ttgccccacc ctgcgcgctg atatacagcg caaaatcacc actgtctgcg
     1141 gcggcgcgtg cagcctcgac gcgctcgtca aactcatcag caatgctgac gccgcgaggc
     1201 aatttgcgta gttcacggta agcccccatt gtcggcaggc caaccgtttt aaattgcgga
     1261 atgcgccacg ttgacgccca tgcggtaaca gccgcggcag tatctttcag cggtctgccg
     1321 ctatcgttat cgagctgccc atccagtgca tagccgtcga tattttttga gatgtatttc
     1381 gcgatatacc ccgcagcacc gccccgatta agatgttttg cctgaaaacg gtttcgcgca
     1441 gctcctcttt cgtcgccatc ctctttgagc gcgtagcgac gcatgatttc gataatctgg
     1501 ttacgctggc gtggattaca aaaaagcatc atatgccagt gcggcgttcc gtcgtggtgt
     1561 ggctcgacga ctcgcaaacc gtagacctgt aaatcattat ccttgaatgc cgtgcgcatc
     1621 aggctccaga tacggcagag ataacgctgc gcatcctttg gattaaatgc ctcatcgttc
     1681 cagccgtgat ttagctggac ggttttactt tcgccttttc caacctgacg tgtcgggtga
     1741 tactttgacg gcgcggtcag cgtgataaac atccccacat caccttctgc ggcggcgtaa
     1801 cgctcaatac cggcaatggt gttcatcagc tccatccggc gaatttcagg attagaaata
     1861 ctgcccatca ccttactgat aaggtcgatg cgctcgcctg tttccctgtt ttcgagatca
     1921 cacgatttaa gaaattccag atttgcctgg cggcgtgcac gcacatcacg aatggcatgt
     1981 ttactggcat aaggagaacg gtctttattg acctccccga cagcaatcag taacgcctca
     2041 tgccagcgca tacgctggcc tttaagctga ctaatccacc actcatcgtt aaacagacgg
     2101 gcaatggcag aatatgcctg cctcgtggtc atctgtcctt tacggtattt tttccagtag
     2161 agaggggaaa tattgaaagc acgtgcagcg ccagcaacat gaccatagag gtgagcctgc
     2221 gcctcatccg taaacagcga ttctttttcg ccatgcgcat ccacccaggc atcgctgagt
     2281 tcctcataca tcatgaaaag ctgcgatgag atacgggcgg caaacttttt cagctccttg
     2341 tcattcattc ccggcaggcg cgcatagtgg tcacgctcag ccagaaacag catcgacgcg
     2401 tcggtgttca tttcatggcg ctgattcaca cgctcaatgc gcggccataa acgacgctga
     2461 aaagtggatg tgaggaaata aaacccgtgc accgggcttt tattgcgccg gatgtagtca
     2521 tagcgtgacg taaacagcga gcgcaaaaag taaggcaggc ggttaatcgt ggataaaaca
     2581 ccttgcacct gacgcatctc gtcacgtgta aggggtcttt cgcgcccgac agcctcgcgt
     2641 ggcgcgttcc atgcataagc accggtaaac gccttaccgg tgcctgcagc aaatgctgac
     2701 ggagggacaa aacgcccgga ggctttaacg gccatatgag ccaaaagcct ctgaacaacg
     2761 cttgctgagt tgctcaacct gcgcgtttaa atcagcaaaa gactttgcgc ttccggtcag
     2821 aatatcgtga tgcatcaggc cggaaacgag ctggcttaat ttcgggtaat aaccaaccac
     2881 cgccagccat tcctgaccgg cgtttttacc gctttccgct ctctttttct cgtggagaat
     2941 aaactggaag ctgtcactgg taacgacata acgttcgcca atttcaatac gaatactcat
     3001 gccgttctcc ggtaatgttt gttttttgct tcaaagactg actgacagga aacacaacgc
     3061 gtggctgacg gataagccgc acgacgggca gcaggaattg gcgcgtcaca ctcttcgcaa
     3121 accagcgcag aagcaccgca atgttttacc cttgccgcgt taatctggcg ctccagtaat
     3181 tcagcctgtt gttcctgaat aaaatctatg ttgtccggca ttatcagctc cttttatcgt
     3241 taagtttcct ggatacatca gcgcaataac tggcaagttc tgtcgttaat tttgtcagtt
     3301 catccactga ggaaatttgc ttgtggaata cagcgcgttt aacaagtaaa ttgaccacat
     3361 cagacaggag gtttaattca ttctgataaa tcgcgataac agattcagtg atgtcgcgtt
     3421 tttctttatc aagacaaagt tgaataagag acaagtcgcc atttttcata acggcgattt
     3481 ttaaggcgtt attcagtaat acaactgaat gagaacagga catcaaagca cctccccgcg
     3541 agacaatccg atattgtgaa atttttccga ctcctgactg agcagctcga ctatctccac
     3601 gcgggataac tccgcctttg tgatatggcg aatcatggcg tcaagatgag aagaaaagcg
     3661 cgtcgcagcg tcggcctgtg cttcggttct ggcctgttgc agcagtaatg cgtatttacc
     3721 gcactgattt tcagaaactg tatgcatgac tttctccagg caaaaagaag ccccgcacaa
     3781 ttaagtgcgt taaaaactct ggttaattat ttaatgcaaa tattgctctg gttttaccga
     3841 cgtcagaatt gtcggtgcat actcaaacag actgaatagt tcacgtaatg cacggaataa
     3901 agcatcacgc cagtaacatg actcttcatt aattcgccag tatggctgat tgaattcttt
     3961 ttcagtcaat ccggcatgca taaataaagt acgacgctga ctgactgtta aaaaactaat
     4021 atatgcatac tcacttgcgc caacctgacg gcgttttgag aatgccccac gcaattcatc
     4081 aattgcacaa accagccgtt cacgttcgac gtcgttcatt tcttcaaaac gcatcgttgc
     4141 gtgacgttgt tttaactgcg catgaaagca aactgttagc cgttcgcgct ccatcatctg
     4201 attataataa tcacatgtat cctgccaacg agggacggca agatgcttac caattatccg
     4261 gcgcatagct gctggctgtt tttcgacgag attgagcgtc atcactgtca tttccagacc
     4321 ctccggcttt tcagaaaggt cagagccttt tttaacggac tctgtttttt ggtgcggata
     4381 atgattccct tgcgaccctt cccgtgggtg atggtgaagt caatcgccct agggctttcg
     4441 ttacgcaata actgagcaat acaacgcggc tcattcataa tcacaacccc atccacaaaa
     4501 gccatgcatc acgctgttca accggtcggt tataaaacgc ctcacgtaca gcgcgattaa
     4561 actcaggaat gaaaacccat ttttcaccgg cacgagcctt cggtttgcaa ggatcacgca
     4621 attcaataat tggtaattta tttgccttca ccatttcact gacggctgtc tttggcttcc
     4681 ctaataaatc agcaaattta tccacatgaa ccgcatcaag cggatactga atcacataat
     4741 tttcagcgtc catatatggt accctcatag gatccagccc tttctaaacc actcaaaacc
     4801 gtttagacgc tggtttattc tcaaatcaat ggaacctata taggttccag ttttgaggga
     4861 atttagtccc tatataggca ccatgtcaaa tgaaattaag cgaaaagatt aaggccttgc
     4921 gtgaggctga agggctaagc caatcaaaat tctgtgaaat catagagtta ccgctaagca
     4981 cacttaaaaa atatgaagga ggaaactttg aacccggtgg cacagctttg ctaaaaatca
     5041 ctatgcatcc cacattccaa aaatatgctc tatggcttat gacagataaa accgcgccgg
     5101 acgcaggaca aatcgcaccg gctctcgcgc acattgggcc agagtcaaca gagtccaacc
     5161 actccgcgaa aaggattggc taactctata taaagattac attttcacca tttgctacca
     5221 agatggtgaa tacagcgccg gagggctttc ttatggcaat taagaagctc gatgatggtc
     5281 gctatgaagt ggacattaga cctcgcggtc gcgacggaaa acgcatccgc aggaaattcg
     5341 aaagaaaagc tgaagcacta gcatttgagc gatacacaat cgccaatgcc agtcagaaag
     5401 aatggggagg ccagcgagca gaccgccgga ctttgagtga gttgctggac atctggtgga
     5461 aatatcacgg gcaaaaccac gagcatggaa caaaagagtt taatcatcta ctcaaaacca
     5521 tcagcggcat aggtgatata ccagtgagca ggatgagcaa aagggctttg atggattatc
     5581 gttccatgcg actacgtgat ggtatcagtg ccgcaacgat aaaccgtgac atgtacagat
     5641 tatccggcat gttcacaaaa ttaattcaat tggatgaatt ttccgggcaa cacccaattc
     5701 acggactgcc gccactggcg gaggccaacc ctgaaatgac gttcctggaa aaagcagaaa
     5761 tcgaaaaact gttaaatgtt ttggctggtg atgacttact tgtcgcgctt ttatgtctga
     5821 gcactggagg aagatggacg aaagttgcca cgctaaaacc agcacagatt acaaattgca
     5881 gggttacctt cctgaaaacc aaaaacggta aaaagcgaac cgtgccgatt tctgaggaac
     5941 tggagaaaaa agttaaagag gaggccagcg ccaaattatt caaagttgat tatgagaaat
     6001 tttgcgggat tttacgcaga gtgaaacctg atataccacc caatcaggca acccacatcc
     6061 tgcggcatac attcgcaagc catttcatga tgaatggggg caatataatc gcactgcaac
     6121 agattctggg acatgcgagc attcagcaga cgatggccta tgcgcacctt gcgcctgact
     6181 atctgcaaaa tgccgtcgct ctgaatccac taaaaggcgg agtgacgtta taaatttccc
     6241 cctctgagtg tccacatagt gtccacactc aagaaatttt gaaaccgctc caaaccctta
     6301 cagctttcga taagtaactg tttttacttt tgattgcatg taagtatttg ataaaaaaaa
     6361 ccccacatca tgtgggggaa gacagggatg gtgtctatgg caaggaaaac agggtttact
     6421 actgggaacg tgagttgcta ctactcaata gcttcaacga tgaacttttt tgccattgcg
     6481 tcacgtcacg caactgctcc attcgttgtt gatgtttctc gtttaaaacc gcttgctgct
     6541 ccggcgttaa caggcgatac atttggttgc ggacttttgc catctcaacc tgacgagcaa
     6601 tttgctcatt cgccattttt tctgcctgtg cgcgcacagc gttttcatca aaattttctg
     6661 cggtgacaag gcgatgcatt gtctccagtt cgctaacatt aacaggaggc tgttcgtgcc
     6721 gggcctgttg cataagatct cgcatctgct gacgctgatg ttcggttaaa cttatgccgt
     6781 cgaacatatg gctctgcgta ctgcgctgcg taagttcttc acccggatgc cagttatcgc
     6841 ctgaaccgac ttcagcagcg tggcttaatg aactgactgc cagcgttgag gccatgacgg
     6901 cagcggtaac tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt caacgataga
     6961 gagtttacga ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt aaagtcatgg
     7021 attagcgacg tctgatgacg taatttctgc ctcggaggta tttaaacaat gaataaaatc
     7081 ctgttagttg atgatgaccg agagctgact tccctattaa aggagctgct cgagatggaa
     7141 ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc ttgatcttct ggacgacagc
     7201 attgatttac ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga cacattaaaa
     7261 gcacttcgcc agacacacca gacgcctgtc attatgttga cggcgcgcgg cagtgaactt
     7321 gatcgcgttc tcggccttga gctgggcgca gatgactatc tcccgaaacc gtttaatgat
     7381 cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag cgagcaacag
     7441 caaaacaacg acaacggttc accgacactg gaagttgatg ccttagtgct gaatccaggc
     7501 cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa ccggtactga gtttaccctg
     7561 ctctatttgc tggcacagca tctgggtcag gtggtttccc gtgaacattt aagccaggaa
     7621 gtgttgggca aacgcctgac gcctttcgac cgcgctattg atatgcacat ttccaacctg
     7681 cgtcgtaaac tgccggatcg taaagatggt cacccgtggt ttaaaacctt gcgtggtcgc
     7741 ggctatctga tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc ttcgccatct
     7801 tctggctgac gctggcgctg gtgttgatgt tggttttgat gttacccaag ctcgattcac
     7861 gccagatgac cgagcttctg gatagcgaac agcgtcaggg gctgatgatt gagcagcatg
     7921 tcgaagcgga gctggcgaac gatccgccca acgatttaat gtggtggcgg cgtctgttcc
     7981 gggcgattga taagtgggca ccgccaggac agcgtttgtt attggtgacc accgaaggcc
     8041 gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg taactttatt ggtcaggccg
     8101 ataacgccga tcatccgcag aagaaaaagt atggccgcgt ggaactggtc ggtccgttct
     8161 ccgtgcgtga tggcgaagat aattaccaac tttatctgat tcgtccggcc agcagttctc
     8221 aatccgattt cattaactta ctgtttgacc gcccgctatt actgctgatt gtcaccatgt
     8281 tggtcagtac gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg gcgcgtaagc
     8341 tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg ccagcacccg gaactggaag
     8401 cggggccaca ggaattcctt gccgcaggtg ccagttttaa ccagatggtc accgcgctgg
     8461 agcgcatgat gacctctcag cagcgtctgc tttctgatat ctctcacgag ctgcgcaccc
     8521 cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg tcgtagcggt gaaagcaagg
     8581 aactcgagcg tattgaaacc gaagcgcaac gtctggacag catgatcaac gatctgttgg
     8641 tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa gccaaccagt
     8701 tgtggagtga agtgctggat aacgcggcgt tcgaagccga gcaaatgggc aagtcgttga
     8761 cagttaactt cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc ctggaaagtg
     8821 cgctggaaaa cattgttcgt aatgctctgc gttattccca tacgaagatt gaagtgggct
     8881 ttgcggtaga taaagacggt atcaccatta cggtggacga cgatggtcct ggcgttagcc
     8941 cggaagatcg cgaacagatt ttccgtccgt tctatcggac cgatgaagca cgcgatcgtg
     9001 aatctggcgg tacaggattg gggctggcga ttgttgaaac cgccattcag cagcatcgtg
     9061 gctgggtgaa ggcagaagac agcccgctgg gcggtttacg gctggtgatt tggttgccgc
     9121 tgtataagcg gagttaaact ccgcatttgt aggcaggata aggcgtttac gccgcatccg
     9181 gcatttgagc aggatgcctg atgcgacgct gatagcgtct tatcaggcct acactccggg
     9241 ggttattttc cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag tcggcgcttt
     9301 tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg agagtaaacg gtgatactga
     9361 tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct cctgcacggt cacatcgctg
     9421 acacgggaaa ccaactccag cggcgcgtcc gcagacactt tccctggcgc tatcacgcta
     9481 tacaaccacc ccaccttacc tgtgttttgc atcaactgcg ctatatcgct gatatcaaaa
     9541 tggtaattga gtttatagca aggcgagcgc ggctggctga cctgaattaa tgcctctccc
     9601 cagcggaaaa tatcgcccat atgaacatta ctttccgtga gaccgtcggt tgagagattt
     9661 tcgccaaacg caggcgcaac aaacaactcc gcctgttccg gaaattcccg cacccagtag
     9721 agatagtgct cacgaggata atgacacagc gctctgtctg gcccgccgtg aacctttttc
     9781 tccgcctgct cgtcaccttc cagccccagc tccgtcagca tcaactcacc atcaacctgg
     9841 attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc tgtgtataca
     9901 tcaaccggat atcgcatctg ttccctctcc acgagaaatt tccccattag accatattta
     9961 ttttatgtca ggcgtgctgt tttcgcggta agtatccagg caggaactca atagcaaacc
    10021 ggacgaacgt tagttcatcc ggctgtgggg attaaggaag caggtttatt tcaccgtacc
    10081 taaaatttcg attttcgctg ctctggcttt gactttacgt gaccagatag aggtaagaat
    10141 tggcaccaga atcgaagtca caatgaccgc cgtcgctacc agcgaagttg ctgccggagc
    10201 catcggttta aacgcaggca ccatttctgc aatcagcaca ggtgtcgcta ccgcggcccc
    10261 tgcggaactg gaagcggcaa ttccggcagt gccatcgcca ccgccaatca atttatcggc
    10321 gataatcaac ggaataccgg tcacgataat tactgccaca cccaacagga tccccagcaa
    10381 accagtctgg gcaatcacag tcaaatcaat ggtattgccc agcgcgaagg caaagaatgg
    10441 aatcagcgtt tgcactgctt tgctgaaaaa ttctcgtaat tcagggtcaa gattccccag
    10501 ggcaaagccc accaggaacg gtaatactgc gccgacgaaa acatgcggtt caaacgaggc
    10561 aatcccggca gtgcccagaa taatcatcgt catgagcggc ccggactcca acgacatcaa
    10621 cacaaatgcc ccagcttctt ctttcgtacc gtactgctgc atgatggaag cgtaaagtcc
    10681 gccgttggtc atatccatcg ccgccaccag cgccagcgtt gaaagtccgg caaagaatcc
    10741 aacttcaaca ccatgttccg gaataatgcg cgaggcaatt gccgcaacca cccacgcgac
    10801 ggcaatttta gttaccacca gcgtaccgga tttacgcagt accgttcccg tcgcgcttaa
    10861 ttttattgac gcccccatgc aaaaaaacca caccgccaga atgggcaccg taccggtaat
    10921 catcccgttg gtgaatgatc caaaatattt ccccgccccc ggcgagaagg tgtggcacag
    10981 tgcgccaagg aatagcggga cgagcatcat cccccccggg attttctcaa tcgagcgttt
    11041 tatctgcatt tccatcacct gtcggcatat ttttaagggc tgatgcgtaa ttcctcaatg
    11101 gagcatcagt gttgatggaa acgaagatag acattcattt aatcaataaa agtgatcaaa
    11161 accacatcaa ttgaaacgct gttttatttt tatcggatca ttgtttttat tttgaaattg
    11221 atcacaaaaa aaccaccgcc gttggcgatg gttcattttt taagctgata tgcggcctat
    11281 acgcctcatt gcagcaggcg gcaaatgatt attttttcgc cgcaaaacgt gccgctgctt
    11341 cgtcccagtt caccacgttc cagaactctt taatgtagtc cggacggcgg ttctggaatt
    11401 tcaggtagta agcatgttcc cacacatcca ggcccagaat cgggaagccg gaagcgccag
    11461 aaatagcttc acccatcagc ggagaatcct ggttagcagt agaaaccacc gccagtttat
    11521 cgcctttcag caccagccat gcccagccgg aaccaaagcg ggaagctgcc gctttttcaa
    11581 attctgcttt gaagttatca acggagccga agtcacgttc gatagccgct ttcaggtcac
    11641 cctgcagggt ggtgcctttt ttcagacctt tccagaacag gctgtggtta gcgtgaccgc
    11701 cagcgttgtt gcgcagtacg gttttcttgt ctgctggcag ctggtccagt ttggtgatca
    11761 gctcttcaac cggcaggttg gcaaattctg gcaggctttc cagcgccgcg ttggcgttgt
    11821 ttacgtaggt ctgatggtgt ttggtgtggt ggatttccat ggtctgctta tcgaagtgcg
    11881 gttccagggc atcgtaagca tacggcaggg atggcagggt atagctcata ttcatctcca
    11941 gtattgtcgg gcggccgatt gttaatgccg cgtaagcagt tggttcatta tagttaatta
    12001 aatgatattg aaaatgatta tcaatgccgt acttttcgta agggtatggt tttgcaggaa
    12061 aatgcccgag atgtgaagca aatcacccac ttaatgccgt gattgccagt aaatcgacaa
    12121 cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt tacactcatc tcgtcggaga
    12181 tgtgaagcga cgaaaaatga tgaggataag aagatgagta acgcgattac gatggggata
    12241 ttttggcatt tgatcggcgc ggccagtgca gcctgttttt acgctccgtt caaaaaagta
    12301 aaaaaatggt catgggaaac catgtggtca gtcggtggga ttgtttcgtg gattattctg
    12361 ccgtggacca tcagcgccct gttactaccg gatttctggg cgtattacag ctcgtttagt
    12421 ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg taatatcaac
    12481 tatggcctga ccatgcgtta tctcggcatg tcgatgggaa ttggcatcgc cattggcatt
    12541 acgttgattg tcggtacgct gatgacgcca attatcaacg gcaatttcga tgtgctgatt
    12601 aacaccgaag gcggacgcat gacgttgctc ggcgttctgg tggcgctgat tggcgtaggg
    12661 attgtaactc gagccgggca gttgaaagag cgcaagatgg gcattaaagc cgaagagttc
    12721 aatctgaaaa aagggctggt gctggcggtg atgtgcggca ttttctctgc cgggatgtcc
    12781 tttgcgatga acgccgcaaa accgatgcat gaagccgctg ccgcacttgg cgtcgatcca
    12841 ctgtatgtcg ctctgccaag ctatgttgtc atcatgggcg gcggcgcgat cattaacctc
    12901 ggtttctgtt ttattcgtct ggcaaaagtg aaggatttgt cgctaaaagc cgacttctcg
    12961 ctggcaaaac cgctgatcat tcacaatgtg ttactctcgg cactgggcgg gttgatgtgg
    13021 tatctgcaat tctttttcta tgcctggggc cacgcccgca ttccggcgca gtatgactac
    13081 atcagttgga tgctgcatat gagtttctat gtattgtgcg gcggtatcgt cgggctggtg
    13141 ctgaaagagt ggaacaatac aggacgccgt ccggtaacgg tgttgagcct cggttgtgtg
    13201 gtgattattg tcgccgctaa catcgtcggc atcggcatgg cgaattaatc tttctgcgaa
    13261 ttgagatgac gccactggct gggcgtcatc ccggtttccc gggtaaacac caccgaaaaa
    13321 tagttactat cttcaaagcc acattcggtc gaaatatcac tgattaacag gcggctatgc
    13381 tggagaagat attgcgcatg acacactctg acctgtcgca gatattgatt gatggtcatt
    13441 ccagtctgct ggcgaaattg ctgacgcaaa acgcgctcac tgcacgatgc ctcatcacaa
    13501 aatttatcca gcgcaaaggg acttttcagg ctagccgcca accgggtaat cagcttatcc
    13561 agcaacgttt cgctggatgt tggcggcaac gaatcactgg tgtaacgatg gcgattcagc
    13621 aacatcacca actgcccgaa cagcaactca gccatttcgt tagcaaacga cacatgctga
    13681 ctactttcat gctcaagctg accgataacc tgccgcgcct gcgccatccc cacgctacct
    13741 aagcgccagt gtggttgccc tgcgctggcg ctaaatcccg gaatcgctcc ctgccagtca
    13801 agattcagct tcagccgctc cgggcaataa ataatattct gcaaaaccag atcgttaacg
    13861 gaagcgtagg agtgtttatc gtcagcatga atataaaaga gatcgccacg ggtaatgcga
    13921 taaggacgat cgttgagtac atgcagacca ttaccgcgcc agacaatcac cagctcacaa
    13981 aaatcatgtg tatgttcggc aaagacatct tgcggataac ggtcagccac agcgactgcc
    14041 tgctggtcgc tggcaaaaaa atcatctttg agaagtttta actgatgcgc caccgtggct
    14101 acctcggcca gagaacgaag ttgattattc gcaatatggc gtacaaatac gttgagaaga
    14161 ttcgcgttat tgcagaaagc catcccgtcc ctggcgaata tcacgcggtg accagttaaa
    14221 ctctcggcga aaaagcgtcg aaaagtggtt actgtcgctg aatccacagc gataggcgat
    14281 gtcagtaacg ctggcctcgc tgtggcgtag cagatgtcgg gctttcatca gtcgcaggcg
    14341 gttcaggtat cgctgaggcg tcagtcccgt ttgctgctta agctgccgat gtagcgtacg
    14401 cagtgaaaga gaaaattgat ccgccacggc atcccaattc acctcatcgg caaaatggtc
    14461 ctccagccag gccagaagca agttgagacg tgatgcgctg ttttccaggt tctcctgcaa
    14521 actgctttta cgcagcaaga gcagtaattg cataaacaag atctcgcgac tggcggtcga
    14581 gggtaaatca ttttcccctt cctgctgttc catctgtgca accagctgtc gcacctgctg
    14641 caatacgctg tggttaacgc gccagtgaga cggatactgc ccatccagct cttgtggcag
    14701 caactgattc agcccggcga gaaactgaaa tcgatccggc gagcgataca gcacattggt
    14761 cagacacaga ttatcggtat gttcatacag atgccgatca tgagcgcgta cgaaacagac
    14821 cgtgccaccg gtgatggtat agggctgccc attaaacaca tgaatacccg tgccatgttc
    14881 gacaatcaca atttcatgaa aatcatgatg atgttcagga aaatccgcct gcgggagccg
    14941 gggttctatc gccacggacg cgttaccaga cggaaaaaaa tccacactat gtaatacggt
    15001 catactggcc tcctgatgtc gtcaacacgg cgaaatagta atcacgaggt caggttctta
    15061 ccttaaattt tcgacggaaa accacgtaaa aaacgtcgat ttttcaagat acagcgtgaa
    15121 ttttcaggaa atgcggtgag catcacatca ccacaatgaa gcaaattgtg aacatcatca
    15181 cgttcatctt tccctggttg ccaatggccc attttcctgt cagtaacgag aaggtcacga
    15241 attcaggcgc tttttagact ggtcgtaatg aaattcagca ggatcacatt atgacctttc
    15301 gcaattgtgt cgccgtcgat ctcggcgcat ccagtgggcg cgtgatgctg gcgcgttacg
    15361 agcgtgaatg ccgcagcctg acgctgcgcg aaatccatcg ttttaacaat ggcctgcata
    15421 gccagaacgg ttatgtcacc tggaatgtgg atagcctgga aagtgccatt cgccttggat
    15481 taaacaaggt gtgcgaggaa gggattcgta tcgatagcat tgggattgat acctggggcg
    15541 tggactttgt gctgctcgac caacagggtc agcgtgtggg cctgcccgtt gcttatcgcg
    15601 atagccgctc caatggccta atggcgcagg cacagcaaca actcggcaaa cgcgatattt
    15661 atcaacgtag cggcatccag tttctgccct tcaatacgct ttatcaactg cgtgcgctga
    15721 cggagcaaca acctgaactt attccacaca ttgctcacgc tctgctgatg ccggattact
    15781 tcagctatcg cctgaccggc aagatgaact gggagtacac caacgccacg accacgcaac
    15841 tggtcaatat caatagcgac gactgggacg agtcgctact ggcgtggagc ggggctaaca
    15901 aagcctggtt tggtcgcccg acgcatccgg gtaatgtcat tgggcactgg atttgcccgc
    15961 agggtaatga gattccggtg gtcgccgttg ccagccatga taccgccagc gcggttatcg
    16021 cctcgccgtt aaacggttca cgcgctgctt atctctcttc tggcacctgg tcattgatgg
    16081 gcttcgaaag ccagacgcca tttaccaatg acacggcgct ggcagccaac ataaccaatg
    16141 aaggcggggc ggaaggtcgc tatcgggtgc tgaaaaatat tatgggctta tggctgcttc
    16201 agcgagtgct tcaggagcgg caaatcaacg atctcccggc gcttatcgcc gcgacacagg
    16261 cacttccggc ctgccgcttc atcatcaatc ccaatgacga tcgctttatt aatcctgagg
    16321 cgatgtgcag cgaaattcag gctgcgtgtc gggaaatggc gcaaccgctc ccggaaagtg
    16381 atgctgaact ggcgcgctgc attttcgaca gtctggcgct gctgtatgcc gatgtgttgc
    16441 atgagctggc gcagctacgc ggtgaagatt tctcgcaact gcatattgtc ggcggcggct
    16501 gccagaacac gctgctcaac cagctatgtg ccgatgcctg cggtattcgg gtgatcgccg
    16561 ggcctgttga agcctcgacg ctcggcaata tcggcatcca gttaatgacg ctggatgaac
    16621 tcaacaatgt ggatgatttc cgtcaggtcg tcagcaccac cgcgaatctg accaccttta
    16681 cccctaatcc tgacagtgaa attgcccact atgtggcgca gcttcactcg acacgacaga
    16741 caaaggagct ttgcgcatga ccactcaact ggaacaggcc tgggagctgg cgaaacagcg
    16801 tttcgcggcg gtggggattg atgtcgagga ggcgctgcgc caacttgatc gtttacccgt
    16861 ttcaatgcac tgctggcagg gcgatgatgt ttccggtttt gaaaacccgg aaggttcgct
    16921 gaccggtggg attcaggcta ctggtaatta tccgggcaaa gcgcgtaatg ccagtgagct
    16981 acgagccgat ctggaacagg ctatgcggct gattccgggg ccgaaacggc ttaatttaca
    17041 tgccatctat ctggaatcag acacgccagt ctcgcgcgac cagatcaaac ctgagcactt
    17101 caaaaactgg gttgaatggg cgaaagccaa tcagctcggt ctggatttta acccctcctg
    17161 tttctcgcat ccgctaagcg ccgatggctt tacgctctcg catcccgacg acagcattcg
    17221 ccagttctgg attgatcact gcaaagccag ccgtcgcgtt tcggcctatt ttggcgagca
    17281 actcggcaca ccatcggtga tgaacatctg gatcccggat ggtatgaaag atatcaccgt
    17341 tgaccgtctc gccccgcgtc agcgtctgct ggcagcactg gatgaggtga tcagcgagaa
    17401 gctggatccg gcgcaccata tcgacgccgt tgagagcaaa ttgtttggca ttggcgcaga
    17461 gagctacacg gttggctcca atgagtttta catggggtat gccaccagcc gccagactgc
    17521 gctgtgcctg gacgccgggc atttccaccc gactgaagtg atttccgaca agatttccgc
    17581 cgccatgctg tatgtaccgc agttgctgct gcacgtcagc cgtccggttc gctgggacag
    17641 cgatcacgtg gtgctgctgg atgatgaaac ccaggcgatt gccagtgaga ttgttcgtca
    17701 cgatctgttc gaccgggtgc atatcggcct cgacttcttt gatgcctcta tcaaccgcat
    17761 tgccgcgtgg gtcattggta cacgcaatat gaaaaaagcc ctgctgcgtg cgttgctgga
    17821 acctaccgct gagctgcgca agctggaagc ggcgggcgat tacactgcgc gtctggcgct
    17881 actggaagaa cagaaatcgt tgccgtggca ggcggtctgg gaaatgtatt gccaacgtca
    17941 cgatacgcca gcaggtagcg aatggctgga gagcgtgcgg gcttatgaga aagcgatttt
    18001 gagccagcgt gggtaaacac tgccggatgc ggcgcgagcg ccttatccgg cctacaggtc
    18061 gaatgaccat gcgtgctgcc accctcaccc cggccctctc cctgaaaggg cgagggagaa
    18121 aaacatagcc tcgtgcggca acggagctga acaccgctcc ggacagtccc ctcgccccta
    18181 cggggagagg gttagggtga ggggaacaag ccgcaccatg tctaaacaag gcaacctaat
    18241 ttacaggaac acagaacatg caaaacatta cccagtcctg gtttgtccag ggaatgatca
    18301 aagccaccac cgatgcctgg ctgaaaggct gggacgagcg caatggtggt aacctgacgc
    18361 tgcgcctgga tgacgccgat atcgcgcctt atcacggcaa ctttcatgca caaccgcgct
    18421 atattcccct cagccaaccc atgcctttac tggcaaatac gccgtttatt gtcaccggct
    18481 ccggcaaatt cttccgtaac gtccagcttg atcctgcagc taacttaggc gtcgtaaaag
    18541 tcgacagcga cggcgcgggc taccatattc tctgggggtt aaccaacgaa gccgttccaa
    18601 cttccgaact tccggctcac ttcctttccc actgcgagcg cattaaagcc accaacggta
    18661 aagatcgggt gatcatgcac tgccacgcca ccaacctgat cgccctcacc tatgtacttg
    18721 aaaacgacac cgcggtcttt actcgccaac tgtgggaagg cagcaccgag tgtctggtgg
    18781 tattcccgga tggcgttggc attctgccgt ggatggtgcc cggcacggac gaaatcggcc
    18841 aggcgaccgc acaagagatg caaaaacatt cgctggtgtt gtggcccttc cacggcgtct
    18901 tcggcagcgg gtcgacgttg gatgaaacat tcggtttaat cgacaccgca gaaaaatcag
    18961 cacaagtatt agtgaaggtt tattcgatgg gcggcatgaa acagaccatc agccgtgaag
    19021 agttgatagc gctcggcaag cgcttcggcg ttacaccact agctagtgcg ctggcgctgt
    19081 aaggagcaaa catgatccgc aaagcctttg tcatgcaggt taaccccgac gcccacgaag
    19141 agtatcagcg tcggcataac cccatctggc cggaactgga agcagtgttg aaatctcacg
    19201 gtgcgcataa ctacgccatt tacctcgaca aagcgcgtaa tctgctgttt gccacggtag
    19261 agattgaatc tgaagaacgg tggaatgcgg tagccagcac tgatgtttgc caacgttggt
    19321 ggaaatatat gaccgatgtt atgcccgcta acccggataa cagcccggtg agtagcgagc
    19381 tgcaagaagt gttttacctg ccgtaattgc ccaatgtgcc agagatgcct
//
LOCUS       DAZRUF010000060        19199 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZRUF010000060 DAZRUF010000000
VERSION     DAZRUF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19199
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.060"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..72
                     /locus_tag="ACHXOL_001697"
     CDS             <1..72
                     /locus_tag="ACHXOL_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808701.1"
                     /translation="ARRRCDVLFAMMRDGTFYTPQGS"
     gene            481..930
                     /gene="alaE"
                     /locus_tag="ACHXOL_001698"
     CDS             481..930
                     /gene="alaE"
                     /locus_tag="ACHXOL_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR6808702.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(967..1311)
                     /locus_tag="ACHXOL_001699"
     CDS             complement(967..1311)
                     /locus_tag="ACHXOL_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR6808703.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1463..1792
                     /locus_tag="ACHXOL_001700"
     CDS             1463..1792
                     /locus_tag="ACHXOL_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR6808704.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            2041..2286
                     /gene="nrdH"
                     /locus_tag="ACHXOL_001701"
     CDS             2041..2286
                     /gene="nrdH"
                     /locus_tag="ACHXOL_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR6808705.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2283..2693
                     /gene="nrdI"
                     /locus_tag="ACHXOL_001702"
                     /pseudo
     CDS             2283..2693
                     /gene="nrdI"
                     /locus_tag="ACHXOL_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            2666..4810
                     /gene="nrdE"
                     /locus_tag="ACHXOL_001703"
     CDS             2666..4810
                     /gene="nrdE"
                     /locus_tag="ACHXOL_001703"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR6808706.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVQSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4820..5779
                     /gene="nrdF"
                     /locus_tag="ACHXOL_001704"
     CDS             4820..5779
                     /gene="nrdF"
                     /locus_tag="ACHXOL_001704"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR6808707.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6135..7337
                     /gene="proV"
                     /locus_tag="ACHXOL_001705"
     CDS             6135..7337
                     /gene="proV"
                     /locus_tag="ACHXOL_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR6808708.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7330..8394
                     /gene="proW"
                     /locus_tag="ACHXOL_001706"
     CDS             7330..8394
                     /gene="proW"
                     /locus_tag="ACHXOL_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR6808709.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8452..9444
                     /gene="proX"
                     /locus_tag="ACHXOL_001707"
     CDS             8452..9444
                     /gene="proX"
                     /locus_tag="ACHXOL_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR6808710.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9735..10919
                     /locus_tag="ACHXOL_001708"
     CDS             9735..10919
                     /locus_tag="ACHXOL_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6808711.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            11043..11780
                     /gene="ygaZ"
                     /locus_tag="ACHXOL_001709"
     CDS             11043..11780
                     /gene="ygaZ"
                     /locus_tag="ACHXOL_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR6808712.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11770..12105
                     /gene="ygaH"
                     /locus_tag="ACHXOL_001710"
     CDS             11770..12105
                     /gene="ygaH"
                     /locus_tag="ACHXOL_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR6808713.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12196..12726
                     /gene="emrR"
                     /locus_tag="ACHXOL_001711"
     CDS             12196..12726
                     /gene="emrR"
                     /locus_tag="ACHXOL_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR6808714.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12853..14025
                     /gene="emrA"
                     /locus_tag="ACHXOL_001712"
     CDS             12853..14025
                     /gene="emrA"
                     /locus_tag="ACHXOL_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR6808715.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYCFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            14042..15580
                     /gene="emrB"
                     /locus_tag="ACHXOL_001713"
     CDS             14042..15580
                     /gene="emrB"
                     /locus_tag="ACHXOL_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR6808716.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15644..16159)
                     /gene="luxS"
                     /locus_tag="ACHXOL_001714"
     CDS             complement(15644..16159)
                     /gene="luxS"
                     /locus_tag="ACHXOL_001714"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR6808717.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16309..17865)
                     /gene="gshA"
                     /locus_tag="ACHXOL_001715"
     CDS             complement(16309..17865)
                     /gene="gshA"
                     /locus_tag="ACHXOL_001715"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR6808718.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            complement(17938..18366)
                     /locus_tag="ACHXOL_001716"
     CDS             complement(17938..18366)
                     /locus_tag="ACHXOL_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR6808719.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18363..18929)
                     /gene="yqaB"
                     /locus_tag="ACHXOL_001717"
     CDS             complement(18363..18929)
                     /gene="yqaB"
                     /locus_tag="ACHXOL_001717"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR6808720.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(18959..>19199)
                     /locus_tag="ACHXOL_001718"
     CDS             complement(18959..>19199)
                     /locus_tag="ACHXOL_001718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808721.1"
                     /translation="TFSNEGIEEIAVLTAGTRKDNVAPATARRANVSESSSRMHHLLQ
                     KISFKNISFLNNSFIHFLNFLLLRHQRQNQAITLK"
BASE COUNT         4572 a         4965 c         4846 g         4816 t
ORIGIN      
        1 gcgagacgac gctgcgacgt tctgttcgcc atgatgcgcg acgggacttt ttataccccg
       61 caggggtcat aacatgcttg acaacttaat aggggcaccc ccctcactgg tgctttctgc
      121 tgttaatgta cattttctgg catcaaaaat gctgtaaatg acacggtcac gacatacacc
      181 gaattctcta cgcttatagc gttacctcac ccccaaactt tcattagtga aattaaaggc
      241 aaggtcatat ctaattgatt taattgaaaa taaatcatat cggcatgtaa aaatccacaa
      301 tgtacaaaaa acgagccgtt acggaatatt ttatctacaa aaactgacta aataaaaatt
      361 tttcactaat tgaatagtca tagccagcga tatacgctat gcgaaaatgc agatggcaat
      421 gagatccact gctttcatct ccattaacat cccattacgc ttttattaag gagcattagc
      481 atgttctcac cgcagtcacg cttgcgtcat gcagttgcag atacgttcgc gatggttgtt
      541 tactgttctg tcgtgaacat gtgtattgaa gttttcctct ccggaatgag cttcgaacag
      601 tctttttatt ccagattggt agcgattccg gtgaacatct taattgcatg gccatacggt
      661 atgtaccgcg atctatttat gcgcgcggca cgcaaagtta gcccgtcggg ctggataaaa
      721 aatctggctg atatcctggc ttatgtgacg ttccagtcac cggtgtatgt ggcgatcttg
      781 ttagtggtgg gcgcagactg gcatcagatt atggcggcgg tcagttcaaa catcgttgtt
      841 tcgatgttga tgggggcggt ttatggctac ttcctcgatt attgccgccg actgtttaaa
      901 gtcagccatt accagcaggt aaaagcctga ctcttctttt cgcgactggc atcgccagtc
      961 gcgtctttaa ctggcttcgc caaataaacc attcaaataa cgttcaagcg caacacgcga
     1021 actgaagcca tgcgggatac cgtaatgctc gtgtcgctcg cctgctaaat agagcggaaa
     1081 ttgttgataa tgatcgcaag ttttaatatc ggaagcgggt tcagccagtg tcgaactacg
     1141 ttcttttaat gttggatgaa ttaccagtgc ggtacgtccc attcgcgctt cgcgattaac
     1201 atagacataa ttttccccac ggcggtaacc ataagctttc tgggttacca catcgacagt
     1261 aaaaccgact ttttcaagta cgcgcgccac ctcgtctggt cgtaaataca tatttgatcc
     1321 tcgttattat caaccgcggg cctaccttac ctgattgcgc attcacaacg ctttcagaaa
     1381 agtccataaa gccgcgatga acctcagtta agaaaattat ggtctacact gaaaattaca
     1441 tcgaattcta atgggggatc atatgtttaa ccgaccgaac cgcaacgacg ttgatgatgg
     1501 cgtgcaggat atccagaatg atgtcaatca attagctgac agcctcgaat ctgtattgaa
     1561 gtcctggggc agcgacgcca aaggggaagc tgaagccgca cgcagtaaag ctcaggcatt
     1621 gctgaaagaa accagggcgc gaatgcatgg tcgtactcgc gtccagcaag ccgcacgcga
     1681 tgccgttggc tgcgcggatt cttttgttcg tgaaagaccc tggtgtagcg tgggtacagc
     1741 agctgcggta ggtattttta tcggcgcact gttaagcatg cgcaaatcgt agtgcaaaaa
     1801 tgataataaa tacgcgtctt taaccccgaa gcctgtcttc ggggtttctt tttgcctggt
     1861 gaatcacaaa aatccccctc ccccgtcacg ctcatatcta gggtaatttc gatcactatt
     1921 tgctatatat tgtgtggttg aatctttttt tcaactacat ctagtatctc tgtatcaaca
     1981 gagagacaac ccgacgcgta tcatcgcgcc gtatcttcat tttaaacgga aatacgaatc
     2041 atgcgcataa ctatttacac tcgtaacgat tgcgttcagt gccacgccac caaacgggcg
     2101 atggaaaacc ggggctttga ttttgaaatg attaatgtcg atcgcgttcc tgaagcagcg
     2161 gaagcgttgc gtgctcaggg ctttcgtcag ttgccggtag tgattgctgg cgatcttagc
     2221 tggtctggtt tccgtccgga catgattaac cgtctgcatc cagcgccaca cgcggccagt
     2281 gcatgagcca gctcgtctac ttctccagca gctccgaaaa cacgcagcgt tttatcgaac
     2341 gtttaggtct gcccgcggtg cgcatcccgc tcaatgagcg ggaacggatt caggtagacg
     2401 agccttacat cctgatcgtg ccctcttacg gcggcggcgg tacggctggc gcggtgccac
     2461 gataggtaat tcgcttttta aacgacgagc acaaccgggc gttgcttcgc ggcgttattg
     2521 cttctggtaa tcgcaacttt ggtgaggcgt atggccgcgc cggagatgtg attgcccgga
     2581 aatgcggcgt gccgtggctg taccgttttg aactcatggg tacgcaaagc gatatcgaaa
     2641 acgttcgtaa aggagtaacc gaattttggc aacgacaacc gcagaatgcc tgacgcagga
     2701 aacgatggat taccacgcgc tgaatgcgat gcttaacctc tacgatagcg caggtcgcat
     2761 tcagttcgat aaagaccgcc aggccgttga cgcctttatg gcgacgcatg tgcgtccgaa
     2821 cagtgtgacc ttcagtagcc agcagcagcg cctgaactgg ctggtcaacg aaggttacta
     2881 tgatgaaagc gttcttaatc gctactctcg cgactttgtc attacgctgt ttgcccacgc
     2941 acacaccagc ggttttcgtt tccagacatt cctcggggca tggaagtttt acaccagcta
     3001 tacgttgaag acattcgacg gtaaacgtta tctggaagat tttgccgatc gagtcacgat
     3061 ggtggcgctg acgctggcac aaggcgatgc gacgctggcg ttgcagctga ccgatgaaat
     3121 gctgtcagga cgctttcagc cagccacgcc gacattcctc aactgcggta agcagcagcg
     3181 cggcgaactg gtttcctgtt ttctgctgcg tattgaagac aatatggagt cgattggccg
     3241 ggcggtaaat tccgcactgc aactgtcgaa acgcggcggc ggcgtggcat ttttgctgtc
     3301 gaatctgcga gaagcgggcg cgccaattaa acgtattgaa aatcaatctt ctggcgtaat
     3361 tccggtgatg aaaatgctgg aagacgcatt ttcctatgcc aaccaactcg gcgcgcgtca
     3421 gggggcaggt gcagtctatt tacatgctca tcatcccgat attctgcgtt ttctcgatac
     3481 aaaacgggaa aatgccgacg aaaaaatccg cattaaaaca ctgtcgctgg gcgtggtgat
     3541 cccggatatc actttccatc tggcaaaaga gaatgcgcag atggcgctgt tttcgcccta
     3601 tgacgttgag cgagtttatg gcaagccgtt tgccgatatc gccatcagcg aacactatga
     3661 cgaactggtt gccgatgaac gcatccgcaa aaaatacctc aacgcccgtg atttcttcca
     3721 gcggctggca gaaatccagt ttgagtccgg ctatccctac atcatgtatg aagacacggt
     3781 aaaccgtgct aaccctgtcg ccgggcgcat aaatatgagt aatctctgct cagagatttt
     3841 gcaggtcaac tgtgcctcag agtatgacga gaatctcgac tatgcccgca caggccatga
     3901 tatctcctgc aatttaggtt cgttgaatat tgcgcacacc atggattccc ccgattttgc
     3961 ccgcacggta gagactgccg tgcgcggttt aacggcggtg tcagatatga gtcatatccg
     4021 cagcgtgcag tccatcgaag ccggaaatgc cgcctcacac gccatcggac tggggcagat
     4081 gaatttacac ggctatctgg cgcgagaagg catcgcttat ggttcgccgg aagcactgga
     4141 tttcaccaat ctctatttct atgccatcac ctggcacgca ctgcgtacct cgatgttgct
     4201 ggcgcgcgaa cgcggtgaaa ccttcgccgg gttcaaacag tcacgttatg ccagtggtga
     4261 atattttagc caatatctgc aaggtaactg gcagccgaaa acggcgaaag ttggcgaact
     4321 gtttgcccgc agcggtatta cgttacctac ccgtgagatg tgggcgcaac tgcgcgacga
     4381 cgtgatgcgc tacggcattt acaaccagaa tcttcaggcg gtgccgccaa ccggttctat
     4441 ctcttatatc aaccatgcta cgtcgagtat tcatccgatt gtggcgaaag tagagatacg
     4501 caaagagggc aaaacaggac gcgtttacta ccctgccccg tttatgacta acgaaaatct
     4561 ggcgctgtat caggatgctt acgaaatcgg cgcagaaaag atcatcgaca cctacgcgga
     4621 agcgactcgc catgtcgatc agggtttgtc gctgacgctg tttttccccg ataccgccac
     4681 cactcgcgat atcaacaaag cgcagattta cgcctggcgc aaaggtatca aaacgctcta
     4741 ttacattcgc ctgcgtcaga tggcgctgga aggcactgaa attgaaggct gcgtctcctg
     4801 tgcactttaa ggaatatcta tgaaactctc acgtatcagc gccatcaact ggaacaagat
     4861 atctgacgat aaagatctgg aggtgtggaa tcgcctgacc agcaatttct ggctaccaga
     4921 aaaggtgccg ctgtcgaacg atattcctgc ctggcagaca ttaactgtcg tagaacaaca
     4981 actgacgatg cgcgttttta ctggcctgac gctgctcgac acgctgcaaa atgttatcgg
     5041 cgcgccttcg ctgatgcccg atgcactcac gcctcatgaa gaagcggtat tatcgaatat
     5101 cagctttatg gaagcggttc atgcccgctc ttacagttcg attttctcga cgctgtgcca
     5161 gaccaaagat gtcgatgccg cctacgcctg gagtgaagaa aacgcaccgt tgcagcgaaa
     5221 agctcagatt attcagcaac attatcgcgg tgatgatccg ctgaaaaaga aaatcgccag
     5281 tgtgtttctt gaatcttttt tgttctattc cggtttctgg ctgccgatgt atttttccag
     5341 ccgcggaaag ctgaccaata ccgcagacct gatccgtctg attatccgtg atgaagcagt
     5401 ccacggttac tacataggct ataaatatca gaaaaacatg gaaaagatat ctctgggaca
     5461 acgtgaagag ttgaagagtt tcgccttcga tttgttgctg gaactctacg acaacgagct
     5521 gcaatacacc gatgagctgt acgccgaaac cccgtgggct gacgatgtga aagcgtttct
     5581 ctgttacaac gccaataagg ctttgatgaa tctgggctac gaacctttat ttcccgcaga
     5641 aatggcggaa gtgaatccgg caatcctcgc cgcgctttcg ccgaatgccg atgaaaatca
     5701 cgatttcttt tccggttcag gctcctctta tgtgatgggg aaagcggttg aaacagaaga
     5761 tgaagactgg aatttctgag ggtgttattt tcaaaaatat cactacccgc gacagggaaa
     5821 taattcccgc caaatagctt tttatcacgc aaatattttg tggtgatcta cactgatact
     5881 ctgttgcatt attcgcctga aaccacaata ttcaggcgtt ttttcgctat ctttgacaaa
     5941 aaatatcaac ttttctcgat ttgctctcag cccttatatc acgggaaatt ccggcgattt
     6001 gctcgcatca atattcatgc cacatttgcc atcaggggtt gcctcagatt ctcagtatgt
     6061 tagggtagaa aaaagtaact atttccattg ggtaatatat cgacataaac aaataacagg
     6121 aatctttcta ttgcatggca attaaattag aaattaaaaa cctttataaa atatttggtg
     6181 agcatccaca gcgagcattc aaatatatcg aacaaggact ttcaaaagaa caaattctgg
     6241 aaaaaactgg gctatcgctt ggcgtaaaag acgccagtct ggccattgaa gaaggcgaga
     6301 tatttgtcat catgggatta tccggctcgg gtaaatccac aatggtacgc cttctcaatc
     6361 gcctgattga acccacccgc gggcaagtgc tgattgatgg tgtggatatt gccaaaatat
     6421 ccgatgccaa actccgtgag gtgcgcagaa aaaagattgc gatggtcttc cagtcctttg
     6481 ccttaatgcc gcatatgacc gtgctggaca atactgcgtt cggtatggaa ttggccggaa
     6541 ttaatgccga agaacgccgg gaaaaagccc ttgatgcact gcgtcaggtc gggctggaaa
     6601 attatgccca cagctacccg gatgaactct ctggcgggat gcgtcaacgt gtgggattag
     6661 cccgcgcgtt agcgattaat ccggatattt tattaatgga cgaagccttc tcggcgctcg
     6721 atccattaat tcgcaccgag atgcaggatg agctggtaaa attacaggcg aaacatcagc
     6781 gcaccattgt ctttatttcc cacgatctcg atgaagccat gcgtattggc gaccgaattg
     6841 ccattatgca aaatggtgaa gtggtacagg tcggcacacc ggatgaaatt ctcaataatc
     6901 cggcgaatga ttatgtccgt accttcttcc gtggcgttga tattagtcag gtattcagtg
     6961 cgaaagatat tgcccgccgg acaccgaatg gcttaattcg taaaacccct ggcttcggcc
     7021 cacgttcggc actgaaatta ttgcaggatg aagatcgcga atatggctac gttatcgaac
     7081 gcggtaataa gtttgtcggc gcagtctcca tcgattcgct taaaaccgcg ttaacgcagc
     7141 agcaaggtct tgatgcggcg ctgattgatg cgccgttagc agtcgatgca caaacgcctc
     7201 ttagcgagtt gctctctcat gtcggacagg caccctgtgc ggtgcccgtg gtcgacgagg
     7261 accaacagta tgtcggcatc atttcgaaag gaatgctgct gcgcgcttta gatcgtgagg
     7321 gggtaaataa tggctgatca aaataatccg tgggatacca cgccagcggc ggacagtgcc
     7381 gcgcaatccg cagacgcctg gggtacaccg acgactgcac cgactgacgg cggtggtgct
     7441 gactggctga ccagtacgcc tgcgccaaac gtcgagcatt ttaatattct cgatccgttc
     7501 cataaaacgc tgatcccgct cgacagttgg gtcactgaag ggatcgactg ggttgttacc
     7561 catttccgtc ccgtcttcca gggcgtgcgc gttccggttg attatatcct caacagtttc
     7621 cagcaattgc tgctgggtat gccagcgcca gtggcgatta tcgttttcgc tctcatcgcc
     7681 tggcagattt ccggggtcgg aatgggcgtg gcgacgctgg tttcgctgat tgccatcggc
     7741 gcaatcggtg cctggtcgca ggcaatggtg actctggcgc tggtgttaac cgccctgctg
     7801 ttctgtatcg tcatcggttt gccgttgggg atatggctgg cgagaagtcc gcgagcggcg
     7861 aaaattattc gtccactgct tgatgccatg cagaccacgc cagcgtttgt ttatctggta
     7921 ccaatcgtca tgctgtttgg tatcggtaac gtgccgggcg tagtggtgac gatcatcttt
     7981 gctctgccgc cgattatccg tctgacgatt ctggggatta accaggttcc ggcggatctg
     8041 attgaagcct cgcgctcatt cggtgccagc ccgcgccaga tgctgttcaa agttcagtta
     8101 ccgctggcga tgccgaccat tatggcgggc gttaaccaga cgctgatgct ggctctttct
     8161 atggtggtca tcgcctcgat gattgccgtc ggcgggctgg gtcagatggt acttcgcggt
     8221 atcggtcgtc tggatatggg ccttgccacc gttggcggcg tcgggattgt gatcctcgcc
     8281 attatcctcg accgcctgac gcaggccgtt gggcgcgact cacgcagtcg cggcaaccgt
     8341 cgctggtaca ccactggccc tgtcggtctg ctgacccgcc cattcattaa gtaactctgc
     8401 acttgcccgg taacgccggg cattatcacc ctgccaaaaa aaggaataac aatgcgacat
     8461 agcgtacttt ttgcgacagc gtttgccacg cttatctcta cacaaacttt tgcagccgat
     8521 ctgccgggca aaggcattac tgttaatcca gttcagagca ccatcactga agaaaccttc
     8581 cagacgctgc tggtcagtcg tgcactggag aaattaggtt ataccgtcaa taaacccagc
     8641 gaagtggatt acaacgttgg ctacacctca cttgcttccg gcgatgcaac cttcaccgcc
     8701 gtgaactgga cgccgctgca tgacaacatg tacgaagctg ccggtggcga taagaaattt
     8761 tattgtgaag gggtattcgt taacggcgcg gcacagggtt acctgatcga taagaaaacc
     8821 gccgaccagt acaaaatcac caacatcgca caactgaaag atccgaagat cgccaaactg
     8881 ttcgatacca acggcgacgg taaagcggat ttaaccggct gtaacccagg ctggggctgc
     8941 gaaggtgcga tcaaccacca gcttgccgcg tatgaactga ccaacaccgt gacgcataat
     9001 caggggaact acgcagcgat gatggccgac accatcagtc gctacaaaga gggcaaaccg
     9061 gtgttttatt acacctggac gccgtactgg gtgagtaatg agctgaagcc agggaaagat
     9121 gtggtctggt tgcaggtgcc gttctccgca ctgccgggcg ataaaaacgc cgataccaaa
     9181 ctgccgaatg gtgcgaatta tggcttcccg gtcagcacca tgcatatcgt tgccaacaaa
     9241 gcctgggccg agaaaaaccc ggcagcagcg aaactttttg ccattatgca gttgccagtg
     9301 gcagatatta acgcccagaa cgccattatg catgacggca aagcctcaga aggcgatatt
     9361 cagggccatg ttgatggttg gatcaaagcc caccagcagc agttcgatgg ctgggtgaat
     9421 gaggcgctgg cagcgcagaa gtaattttta ttcgggcgga taagtcgttt ttgcctcatc
     9481 cgccattctg tgcacaatgc ctgatgcgac gctggcgcgt cttatcatgc ctacaaaata
     9541 cactcattcc gtatggcgga taaggcgttt tcgccgcatc cgccgttctg tgcacaatgc
     9601 ctgatgcgac gctggcgcgt cttatcatgc ctacaaacca catcgcacat ttcaacaagc
     9661 tatctttcgc ccccattcat caacatccgc tattattgat ttccagctta atcatcacct
     9721 gatgaacaaa aataatgact aaacctaatc atgagcttag cccggcgctg atcgtgctga
     9781 tgtctatcgc caccggtcag gcggtagcca gtaactatta cgcccagcca ttgctcgaca
     9841 ccatcgcgcg taacttttcc ctttccgcca gttcggcagg ctttattgtt accgccgcgc
     9901 agttgggcta tgcagcaggt ctgctgtttc ttgttcccct cggtgatatg tttgaacgcc
     9961 gccgcctgat tgtctcgatg accttactgg cggcaggcgg catgttaatc actgccagca
    10021 gtcagtcgct ggcgatgatg atcctcggta cggcattaac cggtttattc tcagtcgtgg
    10081 cacaaattct ggttccgctg gcagcgacgc tggcttcacc ggataaacgc ggcaaagtgg
    10141 ttggcaccat tatgagcggt ctgctgttgg ggatcttgct ggcacgaacc gttgccgggt
    10201 tactggcaaa cctcggcggc tggcgcaccg tcttttgggt tgcctcggtg ttaatggcac
    10261 tgatggcgct agcattatgg cgtggtctgc cacaaatgaa atcagaaacc cacctcaact
    10321 acccacagtt gttaggttcc gttttcagca tgtttatcag cgataagatc ctccgcaccc
    10381 gcgcattgct gggctgcctg acctttgcca acttcagcat tctctggacc tcaatggcct
    10441 ttttgcttgc cgctccacct tttaactaca gcgatggtgt cattggtctg tttggacttg
    10501 cgggagctgc cggagcgttg ggcgctcgtc cggcgggcgg ttttgccgat aagggtaaat
    10561 cgcaccacac cacaactttc ggtctgctgc tgctattact ttcatggctg gcgatctggt
    10621 ttggtcacac ttccgtactg gcgctgatta tcggcatcct ggtgctggat ctcaccgtac
    10681 agggcgtgca tatcactaac cagacggtaa tttatcgaat acaccctgat gcgcgtaatc
    10741 gcctgaccgc aggttacatg accagctact ttattggcgg tgccgccggt tcgctaattt
    10801 cagcctcagc ctggcaacat ggcggttggg ctggcgtttg tctggctggc gcgacgattg
    10861 ccctagttaa cttactggtc tggtggcgag gttttcatcg tcaggaagcc gcaaattaag
    10921 caatcagcaa ccgttttcgt ggctttacac ttataagggt gttaagaagc ccatcagtct
    10981 gataaggtta agatattcat tcagtctatt tataatatta acaatcgtta agcgtacact
    11041 ctatggaaag ccctactcca cagcctgctc ctggttcggc gaccttcatg gaaggatgca
    11101 aagacagttt accgattgtt attagttata ttccggtggc ctttgcgttc ggtctgaatg
    11161 cgacccgtct gggattctct cctctcgaaa gcgttttttt ctcctgcatc atttatgcag
    11221 gcgcgagcca gttcgtcatt accgcgatgc tggcagccgg gagtagtttg tgggttgctg
    11281 cactgaccgt catggcaatg gatgttcgcc atgtgttgta tggcccgtca ctgcgtagcc
    11341 gtattattca gcgtctgcaa aaatcgaaaa ccgccctgtg ggcgtttggc ctgacggatg
    11401 aggtttttgc cgccgcaaca gcaaaactgg tacgcaataa tcgccgctgg agcgagaact
    11461 ggatgatcgg cattgccttc agttcatggt catcgtgggt atttggtacg gtaatagggg
    11521 cattctccgg cagcggcttg ctgcaaggtt atcccgccgt tgaagcagca ttaggtttta
    11581 tgcttccggc actctttatg agtttcctgc tcgcctcttt ccagcgcaaa caatctcttt
    11641 gcgttaccgc agcgttagtt ggtgcccttg caggcgtaac gctattttct attcccgtcg
    11701 ccattctggc aggcattgtc tgtggctgcc tcactgcgtt aatccaggca ttctggcaag
    11761 gagcgcccga tgagctatga ggttctgctg cttgggttac tggttggcgc ggcgaattat
    11821 tgcttccgct atttgccgct gcgcctgcgt gtgggtaatg cccgcccaac caaacgtggc
    11881 gcggtaggta ttttgctcga caccattggc atcgcctcga tatgcgctct gctggttgtc
    11941 tctaccgcac cagaagtgat gcacgataca cgccgtttcg tgcccacgct ggtcggcttc
    12001 gcggtactgg gtgccagttt ctataaaaca cgcagcatta tcatcccaac actgcttagt
    12061 gcgctggcct atgggctcgc ctggaaagtg atggcgatta tataacccac aagaatcatt
    12121 tttctaaaac aatacattta ctttatttgt cactgtcgtt actatatcgg ctgaaattaa
    12181 tgaggtcata cccaaatgga tagttcgttt acgcccattg aacaaatgct aaaatttcgc
    12241 gccagccgcc acgaagattt tccttatcag gagatccttc tgactcgtct ttgcatgcac
    12301 atgcaaagca agctgctgga gaaccgcaat aaaatgctga aggctcaggg gattaacgag
    12361 acgttgttta tggcgttgat tacgctggag tctcaggaaa accacagtat tcagccttct
    12421 gaattaagtt gtgctcttgg atcatcccgt accaacgcga cgcgtattgc cgatgaactg
    12481 gaaaaacgcg gttggatcga acgtcgtgaa agcgataacg atcgccgctg cctgcatctg
    12541 caattaacgg aaaaaggtca cgagtttttg cgcgaggttt taccaccgca gcataactgc
    12601 ctgcatcaac tctggtccgc gctcagcaca acagaaaaag atcagctcga gcaaatcacc
    12661 cgcaaattgc tctcccgtct cgaccagatg gaacaagacg gtgtggttct cgaagcgatg
    12721 agctaacgcg tcatctcgct caaaaatcca gatttataaa agaaaaaatg accggccagc
    12781 atcgcaacat gctggccttt ttggcaagca ggtcggctca gccgatgagt taagaagatc
    12841 gtggagaaca atatgagcgc aaatgcggag actcaaaccc cgcagcaacc ggtaaagaag
    12901 agcggcaaac gtaagcgtct gctcctcctt ctcaccttgc tctttataat tattgccgta
    12961 gcgataggga tttattgttt tttggtactg cgtcatttcg aagaaaccga tgacgcatac
    13021 gtggcaggga atcagattca aattatgtct caggtgtctg gcagcgtgac gaaagtctgg
    13081 gccgataaca ccgattttgt aaaagaaggc gacgtgctgg tcactctcga cccgacagat
    13141 gctcgccagg cgtttgaaaa agccaaaact gcactggctt ccagcgttcg ccaaacccac
    13201 cagctgatga ttaacagcaa acagttgcag gcgaatattg aggtgcagaa aatcgccctc
    13261 gcaaaagcac aaagcgacta caaccgccgt gtgccgctgg gcaatgccaa cctgattggt
    13321 cgcgaagagc tgcaacacgc ccgcgacgcc gtcaccagtg cccaggcgca actggacgtc
    13381 gctattcaac aatacaatgc caatcaggcg atgattctgg ggactaaact ggaagatcag
    13441 cctgccgtgc aacaggctgc caccgaagta cgtaacgcct ggctggcgct ggagcgtacc
    13501 cgtattgtca gtccgatgac cggttatgtc tcccgccgcg cggtacagcc tggggcgcaa
    13561 attagcccaa cgacgccgct gatggcggtc gttccagcca ccaatatgtg ggtggatgcc
    13621 aactttaaag agacgcagat tgccaatatg cgtatcggtc agccggtcac tatcaccacg
    13681 gatatttacg gcgatgatgt gaaatacacc ggtaaagtgg ttggtctgga tatgggcaca
    13741 ggtagcgcat tctcactgct tccggcgcaa aatgcgaccg gtaactggat caaagtcgtt
    13801 cagcgtctac ctgtgcgtat cgaactggac cagaaacagc tggagcaata cccgctgcgc
    13861 attggtttgt ccacactggt gagcgtcaat accactaacc gtgacggtca ggtactggca
    13921 aataaagtac gttcaacacc ggtcgcggta agtaccgcgc gtgaaatcag cctggcacct
    13981 gtcaataaac tgatcgacga tatcgtaaaa gctaacgctg gctaatccag aggtgcgtgt
    14041 gatgcaacag caaaaaccgc tggaaggcgc gcaactggtc attatgacga ttgcgctgtc
    14101 actggcgaca ttcatgcagg tgctggactc caccattgct aacgtggcga tccccactat
    14161 cgccgggaat ctgggctcat cgctcagcca gggaacgtgg gtaatcactt ctttcggggt
    14221 ggcgaatgcc atctcgatcc cgcttaccgg ctggctggca aagcgcgtcg gggaagtgaa
    14281 actgttcctt tggtccacca tcgcctttgc tattgcgtcg tgggcgtgtg gtgtctccag
    14341 cagcctgaat atgctgatct tcttccgcgt gattcagggg attgtcgccg ggccgttgat
    14401 cccgctttcg caaagtctat tgctgaataa ctacccgcca gccaaacgct cgatcgcgct
    14461 ggcgttgtgg tcgatgacgg tgattgtcgc gccaatttgc ggcccgatcc tcggcggtta
    14521 tatcagcgat aattaccact ggggctggat attcttcatc aacgtgccga ttggcgtggc
    14581 ggtggtgttg atgacactgc aaactctgcg cggacgtgaa acccgcaccg aacggcggcg
    14641 gattgatgcc gtggggctgg cactgctggt tattggtatc ggcagcctgc agattatgct
    14701 cgaccgcggt aaagagctgg actggttttc atcacaggaa attatcatcc ttaccgtggt
    14761 ggcggtggtg gctatctgct tcctgattgt ctgggagctg accgacgata acccgatagt
    14821 cgatctgtcg ttgtttaagt cgcgcaactt caccatcggc tgcttgtgta tcagcctcgc
    14881 gtatatgctc tacttcggcg ctattgttct gctgccgcag ttgttgcagg aggtctacgg
    14941 ttacacggcg acctgggcag gtttggcctc tgcgccggta gggattattc cggtgatcct
    15001 gtcgccgatt atcggccgct tcgcgcataa actggatatg cggcggctgg taaccttcag
    15061 ctttattatg tatgccgtct gcttctactg gcgtgcctat acctttgaac caggtatgga
    15121 ttttggcgcg tcggcctggc cgcagtttat ccaggggttt gcggtggcct gcttctttat
    15181 gccgctgacc accattacgc tgtctggttt gccaccggaa cgactggcgg cggcatcgag
    15241 cctctctaac tttacgcgaa cgctggcggg gtctatcggc acgtcgataa ccacgaccat
    15301 gtggaccaac cgcgagtcga tgcaccatgc gcagttgact gagtcggtaa acccgttcaa
    15361 cccgaatgcc caggcgatgt acagtcaact ggaagggctt gggatgacgc aacagcaggc
    15421 atcaggctgg attgcccagc agatcaccaa tcaggggctg attatttccg ccaatgagat
    15481 cttctggatg tcagccggga tattcctcgt actgctgggg ctggtgtggt ttgctaaacc
    15541 gccatttggc gcaggtggcg gtggcggcgg tgcgcactaa gtacaactaa gccagttcat
    15601 ttgaactggc ttttttcaat taattgtgaa gatagtttac tgactagatg tgcagttcct
    15661 gcaacttctc tttcggcagt gccagttctt cgttgctgtt gatgcgtacg tcacgttcca
    15721 gaatgctacg cgcaatatcc tgcgcttcct gcaacgagtg catctggtaa gtgccacact
    15781 ggtagacgtt cagttccggg atctgattct gatcctgcac tttcagcacg tcttccattg
    15841 ccgctttcca ggcatcagca acacgctgct catctggcgt accaatcaga ctcatataaa
    15901 aaccggtgcg gcagcccatt ggcgagatat cgataatctc tacaccatta ccgttaagat
    15961 ggttacgcat aaaaccagca aacaggtgct ccagggtatg gatccctctt tctggcatca
    16021 cttctttgtt cggcacgcag aagcgcagat cgaacacggt gattgcgtcg ccatgcgggg
    16081 tgttcattgt tttcgccacc cgaactgcag gcgcttccat ccgggtatga tcgactgtga
    16141 agctatctaa caacggcatt tagccacctc cggtaatttt tttaaaaatt ttctgaactc
    16201 tttcttccca ggcgagtctg agtatatgaa agacgcgcat ttgttatcat catccctgaa
    16261 ttcagagatg aaattttggc cactcacgag tggccttttt cttttctgtc aggtgtgttt
    16321 ttccagccac accgcaaacg gttcggtatc agcggtttcc atttcctgct gacggcgttc
    16381 agatgcctcg cgctcggcta caaaatcctc ttcgcgcaga atttccagcg gctcttcacg
    16441 cagcagatta cggtaggctt ctgcaaacgc tttgcctgtt ccgccaatac cagtatcaat
    16501 catagacctt aagatacggg cagagaaagt cagatcggga ttatcgaagc aggcaaccag
    16561 ttcatcacac actttctgat acgcgtcgcc gccgttaatg ctatccagcg tttgcgcgac
    16621 gcgtttcaga tcgcggaaca gatctttacc cacctgcagt aacgggaact gcgcggtttc
    16681 gcagccgata cccagcgtca ggcccggttt gcgaccttca aggatcaccc ggttccagtt
    16741 aacgcgcgta caggcaagtt cgctactgct catttccggt gcatcagcca gcgcacacca
    16801 gaccataaac aggtcgagga atcgcacctg ctgttcatct acaccaatcg gcgagaacgg
    16861 gttgatgtcc agcgaacgca cttcaatata ttcaatgccg ccacgtaaca gcgcatcaga
    16921 aggtgactcg ccgctgcggg taacgcgttt tggacgaatc ggcgcataca gttcgttttc
    16981 aatctgcagc acgttgctat tgatttgcag cctcttaccg tctttctcaa taccaatctt
    17041 cgcgtactct tccgatggcg ttttgattgc ctgtttaagg cccgctacgt actcgtaaag
    17101 atcgttgaag gtaataccaa gattgctttg cgatttattg gtatagccga gatcgctcaa
    17161 acgaagagag gtcgcatacg gcaggtaata cataccgcac tcggttttct caaacggcag
    17221 cgacgttggt tttccttgca ggaaagaaga acaaatcgcc ggagatgcac caaacagata
    17281 aggaatgacc caaccgaaac gatagtaatt gcggataacg cggaaatagc ccgcagaaat
    17341 tttctctttg gcatcagcgc ccgagatatc accgcacttc gcttgccaga atgccattgg
    17401 caaagagaaa ttgtagtgca cgccggaaat ggtttgcatc agcgcgccgt agcgattttt
    17461 cagcccttca cgatacagcg ttttaaagcg tccggtgtta gaagtgccgt actgtgccag
    17521 ttcgatgtcc tgaccttctg cgatgtagca tggcatactt aacggccaca tccgctcatc
    17581 gcccatattg cgcgccgtat aacgatgcag atcgcgcata aaggtcagca tatgttcaat
    17641 atcaccatcc actggtgtaa tgaattccag caatgcttcc gcaaaatcgg tagtaatcca
    17701 tttgtgcgtc agtgcggaac ctaatgcttc aggatgacct gttgttgcca gtgtgccatc
    17761 agcattaaca cgcaaagttt cgcgctccag cccacgctgt atccccttta acgcctgagg
    17821 atgtttttcc agccaggcca gcgcctgtga tacgtccggg atcaaattga cctcccgcct
    17881 gtcaaaatcg ttttaattag cataactgta atggtgacca tatgtgcagg cctacaatta
    17941 gtgccaccac atcatgccct gaacggtcgc tgctgcaact gcaacatagc gtaacgcttt
    18001 accaaggcac aaaaaaaaga ttaccggtcc ccacgagatg cgcatccatc ccgctaacag
    18061 acacagtaaa tcgccaacca cgggcatcca gcttaataat agcgtgactg caccatagcg
    18121 tttcagccag ccgatagctt tctcttgcca gcgcgatgtt ttacgcaatg gaaagaaacg
    18181 cccaaggata acgttagtta accctccaag gctattaccc attgttgctg ttaaaactaa
    18241 aacccaggga tgactgatcc cggaaagcaa cattgccacc agcacgactt cggagttgcc
    18301 gggtaatagt gtagcgctga gaaaactact ggcaaacaac gagaaaagcg ataacgcttc
    18361 actcacagca agcgaacgtc cacagcgtcc atacctgcgg cacgggccgc ctgaataccg
    18421 aaatcggcat cttcaaagac cacacactgc gtcggttgca cgcccatacg ctgcgcgcac
    18481 aacaaaaatg tgtctggcgc gggtttatgg tgtttgacgt gatcggcagc gacgacggcg
    18541 tcaaaataat ggcgtaatcc caggtgcgcc agcaatgcct cagcgatggc gctttcactc
    18601 cccgttccta cagccattgg gcgacgacca tgccaacttt tcaccacatc aacaagagga
    18661 agcggttcga cgctatccag cagcatactt cttactgctt ctgttttttc acgcgctaac
    18721 gcatgcgggt cgagatcggc ctgattcagc tcaataattg cctgagcaat acgccaggtg
    18781 ggcgatccat taagcgcaat catcgcctga atatcgtact gaagaccgta gtgccctaat
    18841 acttcgcgcc acgctttacg gtgcgtaggc tccgtatcca ggattgtgcc atccatatca
    18901 aaaattaaac ctgcataacg ctcgtacatg gtcttctcgc aagtcgaaaa atatgacgct
    18961 actttagcgt aattgcttga ttttgtcgct gatggcgaag gagaagaaaa ttcaggaaat
    19021 ggataaagga gttatttaga aatgagatat ttttgaagga aattttttgg agaagatggt
    19081 gcatccggga ggatgactca ctgacgttcg cccttcgggc cgttgctggc gcaacgttat
    19141 ccttcctggt gcctgcggtt aataccgcta tctcttcgat accttcattg ctgaaggtt
//
LOCUS       DAZRUF010000061        19144 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZRUF010000061 DAZRUF010000000
VERSION     DAZRUF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.061"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(220..1869)
                     /gene="pgi"
                     /locus_tag="ACHXOL_001719"
     CDS             complement(220..1869)
                     /gene="pgi"
                     /locus_tag="ACHXOL_001719"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR6808722.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2106..2302
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2394..3743
                     /gene="lysC"
                     /locus_tag="ACHXOL_001720"
     CDS             2394..3743
                     /gene="lysC"
                     /locus_tag="ACHXOL_001720"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR6808723.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3995..4267
                     /locus_tag="ACHXOL_001721"
     CDS             3995..4267
                     /locus_tag="ACHXOL_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR6808724.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4400..5272)
                     /gene="rluF"
                     /locus_tag="ACHXOL_001722"
     CDS             complement(4400..5272)
                     /gene="rluF"
                     /locus_tag="ACHXOL_001722"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR6808725.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5620..6457
                     /locus_tag="ACHXOL_001723"
                     /pseudo
     CDS             5620..6457
                     /locus_tag="ACHXOL_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            6527..7333
                     /locus_tag="ACHXOL_001724"
     CDS             6527..7333
                     /locus_tag="ACHXOL_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR6808726.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7343..7750
                     /locus_tag="ACHXOL_001725"
     CDS             7343..7750
                     /locus_tag="ACHXOL_001725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR6808727.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7750..8244
                     /locus_tag="ACHXOL_001726"
                     /pseudo
     CDS             7750..8244
                     /locus_tag="ACHXOL_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            8310..9107
                     /locus_tag="ACHXOL_001727"
     CDS             8310..9107
                     /locus_tag="ACHXOL_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR6808728.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9118..9942
                     /locus_tag="ACHXOL_001728"
     CDS             9118..9942
                     /locus_tag="ACHXOL_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR6808729.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9994..11222
                     /gene="sorE"
                     /locus_tag="ACHXOL_001729"
                     /pseudo
     CDS             9994..11222
                     /gene="sorE"
                     /locus_tag="ACHXOL_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            11605..12294
                     /gene="pepE"
                     /locus_tag="ACHXOL_001730"
     CDS             11605..12294
                     /gene="pepE"
                     /locus_tag="ACHXOL_001730"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR6808730.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12385..14016)
                     /locus_tag="ACHXOL_001731"
     CDS             complement(12385..14016)
                     /locus_tag="ACHXOL_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR6808731.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFCSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14236..17919)
                     /gene="metH"
                     /locus_tag="ACHXOL_001732"
     CDS             complement(14236..17919)
                     /gene="metH"
                     /locus_tag="ACHXOL_001732"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR6808732.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18119..18943
                     /gene="iclR"
                     /locus_tag="ACHXOL_001733"
     CDS             18119..18943
                     /gene="iclR"
                     /locus_tag="ACHXOL_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR6808733.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4509 a         5012 c         5012 g         4611 t
ORIGIN      
        1 atctatttta ggattaacct cacggtatga tttccgttaa atcacagaca aggggattta
       61 tctgataaaa caatgccgac ttattgtcgg cattgttttt aaggttattt aactcctcat
      121 actatgcatt aatgtcgata ttggcatcag gctccggttg ccggatgcgg cgtaaacgcc
      181 ttatccggcc tacatatcgg catcaggcac gcatgatgat taaccgcgcc acgctttata
      241 gcggttaatc agaccattgg tcgagctatc gtggctgctg atttctttgt catctttcag
      301 ctctggcaga atacggttcg ccagttgttt acccaactcc acgccccact ggtcgaaggt
      361 aaagatgttc aggatcacgc cctgagtaaa gattttgtgc tcatacagcg caatcaacgc
      421 acccaggctg aacggagtga tttcgcgcag caggatggag ttggtcgggc ggttaccttc
      481 gaacactttg aacggcacca cgtagtcaag cgttgccgga tctttaccct gatcgcgata
      541 ttcctgctca accacttcgc gggatttacc aaacgccagc gcttcggtct gggcgaagaa
      601 gttagacagc agtttctggt ggtgatcaga gagcgggtta tgggtgatag ccggggcgat
      661 gaaatcgcac ggtaccattt tggttccctg gtggatcagc tggtagaacg cgtgctgacc
      721 gttagtgcct ggttcacccc agataatcgg gccagtctgg taatccacaa cgttaccgtt
      781 acggtcaaca tacttaccgt tggactccat attgccctgc tggaagtacg ccgcgaaacg
      841 gtgcatatac tggtcatacg gcagaatcgc ttcagtttcc gcaccaaaga aattgttgta
      901 ccagatgcca atcagcgcca gcaggacagg caggtttttc tcggcaggcg tggtggagaa
      961 atgcttgtcc atcgcgtgtg cgccagaaag cagttcaacg aagttatcaa agccgatgga
     1021 gagaacaatc gacaggccaa tcgctgacca caaagagtaa cggccgccga cccagtccca
     1081 gaactcgaac atgttggcag tatcaatacc aaactcgcca acggctttgg cattggtgga
     1141 aagcgccgca aagtgttttg caacgtgttt ttcatcacct gccgctttca ggaaccagtc
     1201 acgcgcgcta tgggcgttgg tcatagtttc ctgagtggtg aaggttttag atgctaccag
     1261 gaacagcgtg gtttccgggt ttactttttt cagcacttca gcgatgtgag tcccatcgac
     1321 gttagaaaca aagtgcatgt tcaggtgatt tttgtacgga cgcagtgctt cggtcaccat
     1381 gtacgggccg aggtcagaac caccgatccc gatgttcact acgtcagtga ttgctttgcc
     1441 ggtataaccc ttccactcac cggagataat cgcttccgag aaggttttca tcttctccag
     1501 caccgcgttg acttccggca ttacgtcttt gccatcaacc agaatcgggg tattgctacg
     1561 gttacgcagc gctacgtgca gcacggcgcg gttttcagtg cggttgatct tctcgccaga
     1621 gaacatcgac ttaatcgcgc ccgccagatc gcactctttc gccagatcct gtaatttcgc
     1681 cagcgtctct tcagtgatgc ggtttttgga gtaatccacc agcatctgat cgtcgaatgt
     1741 tgcggagaac ttagaaaaac gatcgccgtc tttagcaaaa agatcggcga tcgtaacgtc
     1801 tttcatttca tcgaagtgtt tctgtagtgc ctgccaggca gcggtctgcg ttggattgat
     1861 gtttttcatt agcaatactc ttctgatttt gagaattgtg actttggaag attgtagcgc
     1921 cagtcacaga aaaatgtgat ggttttagtg ccgttagcgt aatgttgagt gtaaaccctt
     1981 agcgcagtga agcatttatt agctgaacta ctgaccgcca ggagtggatg aaaaatccgc
     2041 atgaccccgt cgttgacaac cgccccgctc accctttatt tataaatgta ctacctgcgc
     2101 tagcgcaggc cagaagaggc gcgttgccca agtaacggtg ttggaggagc cagtcctgtg
     2161 ataacacctg agggggtgca tcgccgaggt gattgaacgg ctggccacgt tcatcatcgg
     2221 ctacaggggc tgaatcccct gggttgtcac cagaagcgtt cgcagtcggg cgtttcgcaa
     2281 gtggtggagc acttctgggt gaaaatagta gcgaagtatc gctctgcgcc cacccgtctt
     2341 ccgctcttcc cttgtgccaa ggctgaaaat ggatcccctg acacgaggta gttatgtctg
     2401 aaattgttgt ctccaaattt ggcggtacca gcgtagctga ttttgacgcc atgaaccgca
     2461 gcgctgatat tgtgctttct gatgccaacg tgcgtttagt agtcctctcg gcttctgctg
     2521 gtatcactaa tctgctggtc gctttagcgg aagggctgga acctggcgag cgattcgaaa
     2581 aactcgacgc tattcgcaac atccagtttg ccattctgga acgtctgcgt tacccgaacg
     2641 ttatccgtga agagattgaa cgtctgctgg agaacattac tgttctggca gaagcggcgg
     2701 cgctggcaac gtctccggcg ctgacagatg agctggtcag ccacggcgag ctgatgtcga
     2761 ccctgctgtt tgtcgaaatc ctgcgcgaac gcgatgttca ggcacagtgg tttgatgtac
     2821 gtaaagtgat gcgtaccaac gaccgatttg gtcgtgcaga gccagatgta gccgcgctgg
     2881 cggaactggc cgcgctgcag ctgctcccac gcctcaatga aggcttagtg atcacccagg
     2941 gatttatcgg tagcgaaaat aaaggtcgta caacgacgct tggccgtgga ggcagcgatt
     3001 atacggcagc cttgctggcg gaggctttac acgcatctcg tgttgatatc tggaccgacg
     3061 tcccgggcat ctacaccacc gatccacgcg tagtttccgc agcaaaacgc attgatgaaa
     3121 tcgcgtttgc cgaagcggca gagatggcaa cttttggtgc aaaagtactg catccggcaa
     3181 cgttgctacc cgcagtacgc agcgatatcc cggtctttgt cggctccagc aaagacccac
     3241 gcgcaggtgg tacgctggtg tgcaataaaa ctgaaaatcc gccgctgttc cgcgcgctgg
     3301 cgcttcgtcg caatcagact ctgctcactt tgcacagcct gaatatgctg cattctcgcg
     3361 gtttcctcgc ggaagttttc ggcatcctcg cgcggcataa tatttcggta gacttaatca
     3421 ccacgtcaga agtgagcgtg gcattaaccc ttgataccac cggttcaacc tccactggcg
     3481 atacgttgct gacgcaatct ctgctgatgg agctttccgc actgtgccgg gtggaagtag
     3541 aagaaggtct ggcgctggtc gcgttgattg gcaatgacct gtcaaaagcc tgcggcgttg
     3601 gcaaagaggt attcggcgta ctggaaccgt tcaacattcg catgatttgt tacggcgcat
     3661 ccagccataa cctgtgcttc ctggtgcccg gcgaagatgc cgagcaggtg gtgcaaaaac
     3721 tgcatagtaa tttgtttgag taaatactgt atggcctgga agctatattt cgggccgtat
     3781 tgattttctt gtcactatgc ttatcaataa acgagcctgt actctgttaa ccaacgtctt
     3841 tatcgagaat aattgccttt aattttttta tctgcatctc taattaatta tcgaaagaga
     3901 taaatagtta agagaaggca aaatgaatat tatcagttct gctcgcaaac gaattcccgt
     3961 acactgaact ctctggctca ctgagggcac catcatggca ctcccccgta ttacccaaaa
     4021 agagatgact gaacgcgagc agcgtgaact taaaacactg ctggatcggg cgcggattgc
     4081 gcatggtcgc gtactgacca actccgaaac caacagcatt aagaaagagt acatcgataa
     4141 attaatggtc gagcgtgagg ctgaagcgaa aaaagcccgc caattgaaga aaaagcaggc
     4201 ttataaaccg gatcctgaag cgtcatttag ctggtcagca aatacgtcga cgcgcggcag
     4261 gcgctaatga acaatcaacg cctgagattt tcactcgtca gtgtgaacac aattttcccc
     4321 tcaccctaac ccttttcccg aaggggcgag gggactgtcc gagcactttt gggccgtttt
     4381 catcagtctg aggcgttaat cagcgcccct tctttttacg ccccggcgag gtaaaacgct
     4441 taccgttgga cgccgggcga ccgccttttt ccgccgtttt ttccatctta acgactggac
     4501 gtttgatgcc cgctgttttc ggtttggcct tcgctttggg cttcgcctcg gaagaggaat
     4561 tttcaatgag cttaaagaga tcgataagct cgtcgtcggt taaatcgcgc cattctccca
     4621 gcggaatgcc gcttaagctg acgttcataa tgcgcgtacg ttccagcttt ttcacttcat
     4681 agccgaaatg ctcgcacatg cgccggatct gacggttcaa cccctgcacc agggtaatgc
     4741 ggaagacaaa cggtgcttct tttttgactt tgcacttttt cgtcaccgtc ccgaggatcg
     4801 gcactcccgc ccccatacca cgaataaatt catcggtaat cggtttatcg accgtcacca
     4861 gatactcttt ctcatgatcg ttacccgcac ggaggatctt attcaccaga tcgccgtgat
     4921 tggtgaggaa aatcagcccc tgggagtctt tatccaggcg gccaatcggg aacacgcgtt
     4981 tgctgtggtt aacgaaatcg acaatgttat cgcgctcgcc atcttcggtg gtgctgacaa
     5041 taccgacggg cttgttcagg gcgataagta ccaaatcttc ggcttcccga ggctcaatca
     5101 actgaccgtt tactttcaca atgtcgccgg gtttcacctg atcaccaatg gtggctcgct
     5161 tgccattaag gaacacattg ccttgctcga tatagcgatc cgcttcgcgg cgtgagcaaa
     5221 ttccgctttc gctgatgtat ttatttaaac ggactgatga gtcgggcagc ataaattctc
     5281 ctgtaaaacg cggaatatac cgtacctctt agcggggaaa aacagggtta tgcggcaacg
     5341 tcgaaattaa atgtgatctg ccacgccttt tatcacaaaa gtgtctccgg aagtacgatg
     5401 caaatttgcc tggcaaaata aggataatca acatattacg aatcgctgca cacctgtgct
     5461 gatgactact acgcagagaa acaaatgtgc aagagaaagc gctatttctc cccatgcaaa
     5521 gagtgaaaaa tcgctttgaa aggccctcat aatggggtta tatcagatca agcggtgaga
     5581 aagcgtattt gcacaaatgt gcagaaccag gaggcggcaa tggaaaacag tgacgatatc
     5641 cgtttgattg tgaagattgc ccaactctat tacgaacagg atatgacgca ggcgcaaatc
     5701 gcgcgcgaac tgggtattta ccgcaccacc atcagccgct tgcttaaacg aggccgcgat
     5761 cagggaattg tcaccatcgc catcaactat gactacaacg aaaatctctg gctggagcag
     5821 caactgaagc aaaagtttgg cttgaaagac gttgtggtgg tgtcgggaaa tgatgaggat
     5881 gaagagactc aactggcgat gatggggtta cacggcgcgc aactgctgga tcgcttgctg
     5941 gagcctggcg atattgtcgg tttttcctag ggtcgcgcgg tgagcgcact ggttgaaaac
     6001 ttgccgcaag cggggcaatc gcggcagtta atctgcgtgc caattattgg cggaccgtcc
     6061 ggtaaactcg aaagccgcta tcacgtaaac acattaacct acagcgtggc agcgaagctg
     6121 aaaggggaat cgcatctcgc ggattttccg gctctgctgg ataacccctt aattcgtaat
     6181 gggatcatgc agtctcagca ctttaaaacc atctctgcct actgggataa tctggatgtc
     6241 gccctggtgg gaattggctc accggccatt cgcgacggcg ctaactggca tgcgttttat
     6301 ggtggtgaag agagtgacga cctgaaagca ggcgcggtat tccattggca ttgccatgag
     6361 tgaagaaaaa tacagcggaa ttgttggtgc actgcgtgga aaatatatta attgtctggt
     6421 aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat gtttaccgta
     6481 ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc aaacgtggtt
     6541 aaatttgcag gataaaatca ttatcgtcac cggcggcgca tccggtattg gtctggcgat
     6601 tgtagaggaa ttattagcac aaggcgcaaa cgtacagatg gtagatattc acggtggcga
     6661 tggtcaatat gaagttcata aaggctatca gttttggccg accgatattt ccagcgccaa
     6721 agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg acggtctggt
     6781 caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca
     6841 gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaaa aaggcgtttt
     6901 tctgatgtcg caggcggtgg cgcgccagat ggtcaaacaa catgatggcg tgattgtgaa
     6961 tgtttcctca gaaagtggac tggaaggctc agaaggccaa agctgttacg ccgctaccaa
     7021 agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg
     7081 tgtggtcggt atcgcgccgg ggattctgga aaaaaccggg ctacgcacgc cggaatatga
     7141 agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa
     7201 aaacgccatt cctattgggc gcgccggaag attagcagaa gtcgctgatt ttgtttgtta
     7261 tctgctgtct gaacgcgcca gctacatcac cggagtaacc actaacattg cgggcggcaa
     7321 aacgcgcgga taaggaggct ttatggtcaa cgccattttt tgtgcccacg gcaaattagc
     7381 ttgcgccatg ctggaatcgg ttcagatggt gtacggcgac gccaatgtcg aggcggttga
     7441 atttgtacca ggcgagaacg ccggagacat cgtagcaaag ctggaaaagt tagtaagcat
     7501 tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc cgtggaatgc
     7561 ggcagcgatg cttgcgatgc gcaatcctcg tctgcgggta atcagcggcc tttccctgcc
     7621 gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac tgtgtgaaca
     7681 cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa cggcagagga
     7741 agatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca cggccaggtc
     7801 actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt
     7861 tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg aatgaaggtt
     7921 aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata ttaggacgaa
     7981 accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg ccagggggta
     8041 aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa acagttaacc
     8101 aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa taatcttggc
     8161 gtcataatgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat cgacaaaata
     8221 aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt taacaggcag
     8281 ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa attattgcta
     8341 tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt cagactcatc
     8401 gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa accggaataa
     8461 tactcggtgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc gcggcgcaat
     8521 ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt ggtcagcaaa
     8581 gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccaa gtgctgacgg
     8641 tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca gcggaagagg
     8701 cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg
     8761 ttgctattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt
     8821 taaacgccat tcccgaattt gtgacccgtg gactgcaaat tgctggcggt tttatcgtgg
     8881 tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg cctttctttt
     8941 tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc ggtggcgtcg
     9001 gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa gctgaacctc
     9061 aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg agccatcatg
     9121 gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa
     9181 caggcgtcct ttaactttga acgtattcac ggactgggct tttgctacga catgatcccc
     9241 gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag gcgacacctg
     9301 gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgtcac tgccgccatg
     9361 gaagaggcgc gggccaacgg cgcggaaatt gatgacggta ccattaacgg catcaaagtc
     9421 ggtctgatgg gaccattggc aggagttggc gatccactgg tctggggaac gctgcgcccg
     9481 attaccgctg cgctcggcgc atctctggca ctttctggca acatcctcgg cccgctgctg
     9541 tttttcttta ttttcaatgc ggtgcgtctg gcgatgaagt ggtatggcct acagctcggc
     9601 tttcgcaaag gggtgaatat cgtcagcgat atgggcggga atgtgctgca aaaactcacc
     9661 gaaggcgcgt cgattctcgg actgtttgtg atgggcgtgc tggtcaccaa atggacgtca
     9721 atcaacgtac cgttggtggt ttcacaaacg catgccgccg atggctccac cgtcatcatg
     9781 accgtgcaga acattctcga ccaactttgc cctggtttgc tggcgctcgg tctgacgcta
     9841 ctgatggttc gtctgctcaa caaaaaaatt aacccggtat ggctgatttt cgccctgttt
     9901 ggcttaggga ttatcggcaa tgcgctgggc ttcctgtcct gattcttcgc cccggcacga
     9961 ctgccggggc catcgctcaa catgaggtgg tttatgaaaa caacagctct gcgtctttat
    10021 ggtaaacgtg atttacgcct ggaaaccttt gaccttcctg aaatgcagga ggatgaaatc
    10081 ctcgcgacgg tggtcactga cagcctgtgc ctctcttcct ggaaagaggc aacctgggtg
    10141 aaaaccataa aaaagtaccc gacgatgtgg cgaccaaccc aatcatcatc ggccacgagt
    10201 tttgcggcga tattctggcc gtgggtaaaa agtggcagca caaattccag ccgggtcagc
    10261 gttatgtgat tcaggccaac ctgaaactcc ctgaccgccc ggactgcccc ggctactcct
    10321 tcccgtgggt gggcggcgaa gccacgcatg tggttattcc caacgaggtc atggaacaag
    10381 attgcctgct ggcatacgac ggcgaaacct attttgaagg ctcgctggtt gaaccgcttt
    10441 cctgcgtgat tggcgcgttc aacgccaact atcatcttca ggaaggtagt tataaccaca
    10501 cgatggggat tcgcccgcaa gggcgcacgc tgatcctcgg cggcaccgga ccaatgggat
    10561 tgttggcgat tgattatgcg ctgcatggac cagttaaccc gtcgctgctc gtcattaccg
    10621 ataccgacaa cgacaaattg agttatgcgc gcaagcacta tccatcagaa ccgcaaacac
    10681 tgattcatta tctcaatgcc accgatgcag catttgatac gttaatggcg ctgagtggcg
    10741 gtcacggctt cgatgatatt ttcgtctttg tgcctaatga aggactggtg actctcgcct
    10801 cttccttgct ggcgacagat ggttgcctga atttcttcgc cggaccgcag gataaacatt
    10861 tcagcgcgcc aattaatttc tacgatgtgc attatgcatt tacccactac gtgggcacgt
    10921 caggcggcaa taccgacgac atgcgcgcgg cggtcaaact gattgaagag aaaaaagtgc
    10981 aggccgcaaa agtggtaaca catattcttg ggctgaatgc cgcgggcgaa accacgcttg
    11041 aattgcctgc cgtcggcggc ggcaaaaagc tggtgtatac cgggaaatac ctgccgctga
    11101 cgtcactcac gcagattcag gatcaagcac tggcggcgat tctggcgcgt catcagggga
    11161 tctggtccgg tgaggcggag caatatctgc tcactcatgc agaggcaatt tcccatgatt
    11221 aatcgcgcta cttaactctg catgtcgctg gctggtcgcc gcggcaactt cgcgacctga
    11281 tgtgcggctg attgctgaag cggcccaatg tgcacgagaa ggataaaaaa acatcagcgc
    11341 cttaaggggc gctcaaattg gtggcaaagt acacattgtt tatgccggat gcggaataaa
    11401 gccttattcg gcctacataa tctcgaaaat tcaatatatt gcagttatct ggtaggcctg
    11461 gtaagcgtag cgcatcaggc aatgctgtgt ttgtcatcag tctcagcgcc cggtgcaata
    11521 cgtgacatcg tgagccatgt cagtaagtgg ttaaattcgc agtggtagca tgccttccgt
    11581 gatttcttac taaccggaga agtaatggaa ctgcttttat tgagtaactc gacgctgccg
    11641 ggtaaagcct ggctggaaca tgcactgccg ctaattgctg aacagttgca gggtcgccgc
    11701 tcagcggtgt ttatcccttt cgctggcgta acgcagacct gggatgatta cacagcgaaa
    11761 acggctgcgg ttctcgctcc gctgggtgtt tctgtcaccg gtattcatag cgttgtcgat
    11821 cccgttgccg cgattgaaaa tgctgagatc gtgattgtcg ggggcgggaa tactttccag
    11881 ttgctgaaac agtgccgcga gcgcgggctg ctggcaccaa ttactgacgt ggttaaacgt
    11941 ggtgcgctgt atattggctg gagcgcaggc gctaaccttg cttgcccaac cattcgtacc
    12001 accaacgata tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct gttcccgctg
    12061 caaatcaacc cgcacttcac caacgcgctg ccggaaggcc ataaaggtga aacccgtgag
    12121 cagcgtattc gcgaactgct ggtcgtcgcg ccagaactga cgattattgg tctaccggaa
    12181 ggtaactgga tcacggtgag taaaggtcac gctacgctgg gtggcccgaa caccacttat
    12241 gtgtttaagg ccggtgaaga agcggtgccg ctggaagcgg gtcaccgttt ttaagtgaat
    12301 tgccggatgc atgatccggc atcttgttca tagtgcctga tgcgacgctt gacgcgtctt
    12361 atcaggccta caggattcac aatattaata ctcgtcccgt ccttcatctt catccggctg
    12421 ttccagcaca ctgtaagcca ccgaacaaaa cagcgagttc agacgctgca tatctcccag
    12481 taagcctaaa tgtagcgaac tggtttcaat gctctgcacg ttttgctgat gcaggcgatc
    12541 gacgtgggcg tgggaatagc ggcgattaag aatgcgaaag cgatgcttgc tgcggcgcaa
    12601 acgacgagcg ctggtgacat cgccagagca gaacaccgac attgccagct ttaaattact
    12661 gagcaattgc tcatagagcg catccagttc cttcaaccca tcaagcgaaa atgcccgccg
    12721 tgctgccagc gatttatcag caatttcgct gcccatgcgc tcgacgatat cggaagcctg
    12781 ttcaaggttg agcgacattt caataatctc cgcccagcga cgcgactctt cttctgccag
    12841 ttcctctttt ggcattcgcg ccagatacag cttaatggcg gtgtagagaa cgttgatatc
    12901 atctgccagc ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca ctttattcag
    12961 cccttccatc atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc
    13021 cagagcaagc gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc gtagctgggt
    13081 atccagttcc ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa
    13141 tggcagcatg accaggcaac gtacaaggtt gtagaagacg tggaaataga tcaccagttc
    13201 cgcttttggc aatgacaact tccccattgt ctctgccagc aaatggacaa acggcaggat
    13261 aatcaggcta cccaccagct taaacagcaa actacccagc gcgacacggc gggctgcggc
    13321 attggcggca ctgttgttga gcatcgccag caggccggaa ccgaggttag caccaatcac
    13381 cagacagagc gccacgggga aggagataat gcctgcggcg gtcagagtcg cggtcagcag
    13441 tacagcagca aggctggagt agctgataat ggcgaacatc gcgccaatca gcgcatccag
    13501 cagaatatcg ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg
    13561 cgttacggcc tgcacaatca actccagcgc cagcaaaatc agcccaagac caataccgac
    13621 gcggcccagt tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa
    13681 aataagtaac ggtgacagcc aggataagtc gaaggtgaga atacgcgcca ttagcgccgt
    13741 cccgacatct gcacccagca caatcaccag agccggtgcg agggctacca gatcctgagc
    13801 gacgaacgag gtcaccagca tggtggtggc attactgctc tgtaccagtg cggtaacgcc
    13861 gatccccgcg caaaaggcga gcggcttttt ttcgacgctc cggctaagga cagtacgcaa
    13921 acgcgcgccg aagacgcgca ttacgccggt tcgaacaata tgagtccccc agaccagcag
    13981 ggcgacggca gaaagcaggt gaagcagcgt taacacgagt tcaggttctc cttatcgtta
    14041 tatgttcctg aagcaattga atgaccaaag gaccggaaac acgctgactg gcatctttac
    14101 tgtgcctttt gacattcgtt tttccggcta agattattcc agcctgtttc cagtataagt
    14161 gtttcatatt aataaagaga cagggcactc attgagcgcc ctgtttgttg taaggaaaag
    14221 tgacagattt gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc agcgctcaac
    14281 atcggagacg ctcatacctt tacggcgggc atagtcttca acctgatcgc gctgaatttg
    14341 cgccaccgcg tagtacttgc tgtccgggtg gctgaaatac caacccgaaa ccgatgcacc
    14401 aggccacatg gcgaaagatt ctgtgagttt catgccagta tgtttttcca cttccagcag
    14461 ctcccagatg gtggcttttt ccgtatgttc cgggcaggcc gggtagcccg gtgccggacg
    14521 gatgccctgg tagttttcgc ggatcagttc ttcgttgctg aggttctcgt tcggcgcata
    14581 gccccagtag actttacgca cacgctcatg gagatactcc gcaaaggctt cggctaaacg
    14641 gtcggcaagc gctttcacca tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc
    14701 agccagtgcg tcctcttcca gcccgccagt cacggcaaat gcgccgatgt aatctgcttt
    14761 accagaaagc ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc ccgttttttc
    14821 ggtctgttga cgcagatggt ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta
    14881 gatttcaatg tcatcgccca cacggtttgc cgggaacaag cccaccacgc cacgcggatt
    14941 cagcgttttc tcggcgctta atttatccag catgtcgttg gcgtctttaa acagccgctg
    15001 cgcctcaacg cccaccactt catcttccag aatgcgcgga tacttcccgg ccagcgacca
    15061 ggtcataaag aacggtgtcc agtcgatgta attacgcaga gtttcgatgc tggcttcaac
    15121 ttcctgcacg ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt caaaagcgaa
    15181 gtcgttatcg cgcgccgctt ccagcgtgac cggtggtgtg cgcggtttct tgcgcccgtg
    15241 ctgaatacgt acggtttcat actccttgcg ggtacgagcg acaaaatcat cgcgctgagt
    15301 atcggaaagc agcgccgcca ccacaccaac ggtgcgcgag gcattctgca catacaccgt
    15361 cgggccgctg tagttctgct caattttcac cgccgtgtgc gcttttgagg tcgtcgcgcc
    15421 accgatcagt aacggaatgg tgaagccctg acgctccatc tctttcgcca cgttaaccat
    15481 ctcgtccagc gacggcgtga taagccccga aagtccaatc agatcagcat tcacttcttt
    15541 agcggtacgg agaatttttt ccgcaggcac cataacgccg agatcgacaa tttcgtagtt
    15601 gttacattgc agcaccacac caacgatatt tttaccgatg tcgtggacgt cgcccttcac
    15661 ggtggcgatc accatcttgc cgttggtttt gccctgctct ttgctggctt caataaacgg
    15721 ttcgaggtag gccactgcct gtttcatgac gcgcgccgat ttgaccacct gtggcaggaa
    15781 cattttcccc tcgccaaaca gatcgccgac cacgttcatg ccgtccatca acgggccttc
    15841 aatcacttca atcgggcgcg tagcctgctg gcgggcttct tcggtatcct gctcgataaa
    15901 ctcagtaatg cctttgacca gcgagtattc cagacgttta ttcacttccc acgagcgcca
    15961 ctccgcctgc tgggcgttag cggtgtcgtc ggttttgctg ccgcgatatt tctcggcaag
    16021 ctccagtaaa cgttcagtgc catcatcgcg acgattaaga atcacatctt ccaccgcgtc
    16081 gcgcagttca gcgggcaggt cgtcgtaaat cgccagttgc ccggcgttga cgatccccat
    16141 atccatgcca ttgcgaatag cgtagtagag gaacaccgcg tgaatggctt cgcgcaccgg
    16201 ttcgttgcca cggaatgaga aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg
    16261 cagttcgcgt ttgatgtctt cgcaggcgcc gataaagtcc tgcgcatagt tgttgtgctc
    16321 ttcaatgccg gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa
    16381 gccaacctct tcggtgagga ttttgtacgc ccgacggcaa atctcgattt tccgtgcgcg
    16441 ggtatcggcc tgtccctgtt cgtcaaaggc cattaccacc accgctgcac cgtaacgacg
    16501 caacagtttc gcatgatgga taaaggcgtc gacgccctct ttcattgaga tagagttaac
    16561 aatgcctttg ccctgaatac acttcagccc cttttcgatg acctcccatt ttgaggagtc
    16621 gatcataatc ggcacgcgag cgatatccgg ttcaccggca atcagattga gaaaacgcac
    16681 catcgccgct tcggcgtcga gcatcccttc atccatattg atatcgataa tctgcgcgcc
    16741 gttttccacc tgctggcggg ccacatccag tgcctcgctg tatttctctt ctttgatcag
    16801 gcgcttgaac ttagcggaac cggtgacgtt ggtgcgttca cccacgttca caaacaggct
    16861 atcttcgcca atgttcagcg gctccaggcc ggacaaacgg caggctacgg ggatttccgg
    16921 cagtttgcgc ggcgctaatc cttctactgc acgactcatc gctgcaatat gttgtggcgt
    16981 ggtgccacag cagccgccga cgatattgag aaagcccgct tccgcccatt cacgtatttg
    17041 ttttgccatc gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta gcccggcgtt
    17101 cgggtgcgcg gtgacgtagc attccgcaat ccgtgacagc tcctgcacgt actggcgcag
    17161 ttcatcgggc cccagcgcac agttcaggcc aaaggtcagc gcttcggcgt ggcgcaatga
    17221 gttgtaaaat gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt cggtgatggt
    17281 gccagagatc ataatcggca gctcaacgcc cagcgtttca aactccgttt ttaccgcaaa
    17341 taccgccgct ttggcgttaa gggtgtcgaa aacggtttca atcaggatca gatccgcacc
    17401 accttccacc agcgctttgg tggactctcg ataagccgcc accagcccgt caaaagtgat
    17461 attacgaaat gccggatcgt tgacgtccgg agaaatagac gccgtgcggt tggttgggcc
    17521 gagaacaccg gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca
    17581 ggcgcgcgcc agtttcgccg ccgcaaagtt gatttccgcc gacagggatt ccatctggta
    17641 atccgccatc gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc
    17701 ttcaaagtag gcgttgtgga tagcggcgat cacttccggt ttactgagta ccagcaggtc
    17761 gttgttgcct ttgaggtcgc atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc
    17821 gttcagtcga tagctctgga tcatggtgcc cataccgccg tccagcacca gaatacgttc
    17881 atttaactgc gcacgcagtt gttccacttt gctgctcaca cttgctcccg acacgctcaa
    17941 cccagattta ataaaaattc aacaaaccat actggcataa acgcatctgt ggtaaaagcg
    18001 accaccacgc aacatgagat ttgttcaaca ttaactcatc ggatcagttc agcaactatt
    18061 gcattaacta acaataaaaa tgaaaatgat ttccacgata cagaaaaagg agactgtcat
    18121 ggtcgcaccc attcccgcga aacgcggcag aaaacccgcc gttgccaccg caccagcgac
    18181 tggacaggtt cagtctttaa cgcgtggcct gaaattactg gagtggattg ccgaatccaa
    18241 tggcagtgtg gcactcacgg aactggcgca acaagccggg ttacccaatt ccacgaccca
    18301 ccgcctgcta accacgatgc aacagcaggg tttcgtgcgt caggttggcg aactgggaca
    18361 ctgggcaatc ggcgcacatg cctttatggt cggcagcagc tttctccaga gccgtaattt
    18421 gttagcgatt gttcacccta tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa
    18481 tatggcggtg cttgatcaaa gcgatcacga agcgattatt atcgatcagg tacagtgtac
    18541 tcacctgatg cgtatgtccg cacctatcgg cggtaaattg ccgatgcacg cttccggtgc
    18601 aggtaaagcc tttttagccc aactgagcga agaacaggtg acgaagctgc tgcaccgcaa
    18661 agggttacat gcctataccc acgcaacgct ggtgtctcct gtgcatttaa aagaagatct
    18721 cgcccaaacg cgcaaacggg gctattcatt tgacgatgag gaacatgcac tggggctacg
    18781 ttgccttgca gcgtgtattt tcgatgagca ccgtgaaccg tttgccgcaa tttctatttc
    18841 cggaccgatt tcacgtatta ccgatgaccg cgtgaccgag tttggcgcga tggtgattaa
    18901 agcggcgaag gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc tggcgggcag
    18961 aggcaatatt ctgcccatca tacctgagtg gcaatagaat aagggtgtct gttaatcgca
    19021 ttgacgccaa aataacttaa tgtcatacac ttcacacact tttattaact cagcattatt
    19081 tttaaacatc aaaccactta attattaaca aaatcgcaag atctcattcc accgaaactt
    19141 ttac
//
LOCUS       DAZRUF010000062        18842 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZRUF010000062 DAZRUF010000000
VERSION     DAZRUF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.062"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..101)
                     /locus_tag="ACHXOL_001734"
     CDS             complement(<1..101)
                     /locus_tag="ACHXOL_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808734.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTA"
     gene            complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHXOL_001735"
     CDS             complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHXOL_001735"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR6808735.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHXOL_001736"
     CDS             complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHXOL_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR6808736.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHXOL_001737"
     CDS             complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHXOL_001737"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR6808737.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHXOL_001738"
     CDS             complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHXOL_001738"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR6808738.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHXOL_001739"
     CDS             complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHXOL_001739"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR6808739.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHXOL_001740"
     CDS             complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHXOL_001740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR6808740.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKRALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHXOL_001741"
     CDS             complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHXOL_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR6808741.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9871..10449
                     /locus_tag="ACHXOL_001742"
     CDS             9871..10449
                     /locus_tag="ACHXOL_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR6808742.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10493..11968
                     /gene="ampG"
                     /locus_tag="ACHXOL_001743"
     CDS             10493..11968
                     /gene="ampG"
                     /locus_tag="ACHXOL_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR6808743.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12429..13376
                     /gene="cyoA"
                     /locus_tag="ACHXOL_001744"
     CDS             12429..13376
                     /gene="cyoA"
                     /locus_tag="ACHXOL_001744"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR6808744.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13398..15389
                     /gene="cyoB"
                     /locus_tag="ACHXOL_001745"
     CDS             13398..15389
                     /gene="cyoB"
                     /locus_tag="ACHXOL_001745"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR6808745.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15379..15993
                     /locus_tag="ACHXOL_001746"
     CDS             15379..15993
                     /locus_tag="ACHXOL_001746"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR6808746.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15993..16322
                     /locus_tag="ACHXOL_001747"
     CDS             15993..16322
                     /locus_tag="ACHXOL_001747"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR6808747.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16334..17224
                     /gene="cyoE"
                     /locus_tag="ACHXOL_001748"
     CDS             16334..17224
                     /gene="cyoE"
                     /locus_tag="ACHXOL_001748"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR6808748.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17409..17720
                     /locus_tag="ACHXOL_001749"
     CDS             17409..17720
                     /locus_tag="ACHXOL_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR6808749.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17704..18246
                     /locus_tag="ACHXOL_001750"
     CDS             17704..18246
                     /locus_tag="ACHXOL_001750"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR6808750.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18302..>18842)
                     /locus_tag="ACHXOL_001751"
     CDS             complement(18302..>18842)
                     /locus_tag="ACHXOL_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR6808751.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4214 a         4956 c         4581 g         5091 t
ORIGIN      
        1 gccgtcggtt tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc
       61 agggacaggg taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg
      121 ttaacagtgg aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa
      181 tggaaaaggc cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg
      241 cggcttcagg attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc
      301 aggttactca tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc
      361 atcgcttttt tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga
      421 accacattac cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt
      481 ggcagtgcaa acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc
      541 tcgccaaatt tcagaccggc agcctgcata gcttccgccc ctttgccggc tttcaaatca
      601 accagcagtt tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt
      661 gccttaactt gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg
      721 atgcgcagca cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccggc
      781 gcgccgtttt cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc
      841 aactcttccg gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca
      901 gcttgctctg caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc
      961 tgcagcgcat agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca
     1021 cgtacttcgt ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg
     1081 aaaccgaccg aagatttgat gacaccagac agttggcctt tttctttcag accagcattt
     1141 ttcagttcgt cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca
     1201 gagataatat cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc
     1261 tcatcaagta ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta
     1321 cgctgcggct gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg
     1381 ctaaccggtt gctgcatcgt tgcggcatcc agcttgatgt aactcacgcg gaattgttcc
     1441 ggcgtcatga aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca
     1501 ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc
     1561 gcgaccagtg ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaaca
     1621 ccgttaatca gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg
     1681 gtcatcccca tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc
     1741 tggaaggctg gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc
     1801 agctcacgag cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga
     1861 cgcagggttt tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt
     1921 tgctgcatgc gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc
     1981 tggtcattca cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg
     2041 gtcaatatga acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca
     2101 gccgtgcgta agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct
     2161 cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag
     2221 cccacaagac acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc
     2281 ttgaacttcg tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt
     2341 tacctgcacg gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg
     2401 ggttgcggcc agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg
     2461 ctacatcatc cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac
     2521 ggccagccgc agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt
     2581 tattcactct tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa
     2641 cgcgcagcag ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc
     2701 cgatccgcca tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca
     2761 gccctgtttt tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg
     2821 ccagacggtt cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga
     2881 atgtccagat cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac
     2941 ggaattagca ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca
     3001 ccgatcggca gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga
     3061 accgggttac cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga
     3121 ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa
     3181 tcagggttga tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata
     3241 gactcctgca tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca
     3301 catgcggttt caatggtcag caagtcaccg cccacttccg tccacgccag accggttacc
     3361 tgaccgacac ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag
     3421 tcatgcaggt tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc
     3481 ttaaccgctt tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca
     3541 cgggtgtagt aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt
     3601 ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt
     3661 tcatcttcgg tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga
     3721 atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc
     3781 aggtagtggt cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag
     3841 gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcggg
     3901 tttttcacgc ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa
     3961 gtacggcggt gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca
     4021 tatttacgcc cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc
     4081 ggccctacca ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg
     4141 tactcaagga tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc
     4201 tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc
     4261 cagtcgatat aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc
     4321 ttctgcaact ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt
     4381 ttgcgcttca gggcttcgtt ttcgtccggc gcgtcgtcca tttcaccgag ttctttctga
     4441 atagctttca tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta
     4501 acgcggttgc gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc
     4561 atcagatatt ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc
     4621 agtttcagcg gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta
     4681 ttcagcgacg tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac
     4741 tggctgattg cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc
     4801 agatactccg ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg
     4861 cgctgtaacc cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat
     4921 atagaggcca cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct
     4981 tctttctgcg cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg
     5041 atagattttt cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg
     5101 cgcagcggca atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct
     5161 cttagtttaa tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa
     5221 catgtacgtc agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa
     5281 ttaaagggga gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta
     5341 ttcaccagat gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc
     5401 atcaattacc gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta
     5461 catggtatcg agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg
     5521 cgccatcgct ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc
     5581 ttccagatta aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat
     5641 ctgaatcaga gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat
     5701 gaactcaggg ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc
     5761 gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc
     5821 ggtttctaca cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa
     5881 caggatctta gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg
     5941 cggtggaaca gcagctaccg taccttcgat cagtttcaac agtgcctgct gtacgccttc
     6001 accggaaacg tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc
     6061 atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag
     6121 cttctgaatg atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc
     6181 gtcggccatg gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt
     6241 accggaaccg gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct
     6301 ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt
     6361 cgcctgttcc tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg
     6421 tagcgcactg cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt
     6481 acataaatca acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac
     6541 ttcatgctgg cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg
     6601 tttatctgtc atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa
     6661 tgccattctc aggcgcaagc cgcttacagc gttgtgccgc cctggataag tatagcggca
     6721 cagttgcgcc tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc
     6781 accgcttcag gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat
     6841 gattgacccg tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca
     6901 cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc
     6961 atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg cccctgcggt cagcaagaaa
     7021 gcgcccatcg aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc
     7081 atggtgtcat agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga
     7141 tagatatctt tttctgggtt ttccgcttct aggaacagca tctgcgccac aatcaggttc
     7201 gccatgtggt cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa
     7261 tagatatcaa aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc
     7321 atatggggtg caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa
     7381 ttgaaaaaac ctgctgtacc gattgtaacc ttatggacgg attatcagct agtccctgtg
     7441 ttatgggtac ggcatcacca tatttcaagc ataacaatct tttgttgtta cgctaacacc
     7501 gaaagaggct tttcgcacgg ttccatgcaa aattcatgac aaaaaaaagc ccaccacctg
     7561 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
     7621 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
     7681 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
     7741 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
     7801 ctttcacgcg ctcttcgtca gctttcagct cgttggtgcg gataacttcg cccagcagca
     7861 ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag
     7921 ctcgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
     7981 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
     8041 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
     8101 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
     8161 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
     8221 tacctttcag attttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
     8281 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
     8341 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
     8401 tgaagtcgat ggttacgcgg tcttctgctt caacggcgcc gtctttttct ttccaggtcg
     8461 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
     8521 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
     8581 actctacaga gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
     8641 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
     8701 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
     8761 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
     8821 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
     8881 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
     8941 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
     9001 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
     9061 catcactttt atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc
     9121 cgggcaattt tcgtctaaat gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc
     9181 ccgcaggccg tttttcataa aatctttagc aacatactgg aaaagcgccg acagttgcaa
     9241 atgcgttttt acgcgatgct tcctgctcca cgacagggag gagaggcaaa gacggtgtcc
     9301 tgcaaccctt cccactcctt aatagtgtaa gtatgcagag ccagcgcatg aacggtagta
     9361 gagagttcct ccgctaaagt actgtaaatc attcgatgac gattcagaaa acgttcaccc
     9421 gtaaaacgat cgctgaccag cacaacttta aaatggcttt cagagccggc tgggacattg
     9481 tgacgatagc tttcatccac tacttcgagg aatacgggtt ggaacgccgc ccttaatttt
     9541 tcttctatcc gctcacgtat catcatgaaa tatctcctcc gacaacgctg agatgtcatc
     9601 catcccttta aatactagcc gcttttaccg tttccattgc agcttaacaa caaatattta
     9661 ggtttcgtcc tggtttttcc gtcaagcgta tgaagtttac tgatttaaag attgaaacac
     9721 aggcctcgca tcagcgtttc ttttaccagg ggtgattatt tcgccaacat gatgaattta
     9781 catgccttac ccacttcccc tcgccgttgg caatgttatg atggcgggaa tttttaccaa
     9841 acacgcttaa gagtctctga gagcccgaac atgttcaaaa aaatcctctt cccgttagtt
     9901 gctctgttta tgcttgcagg atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg
     9961 attacactgc cacagcagga tccaagcctg atgggcgtca ccgtaagcat taatggtgcc
    10021 gatcagcgta ccgatcaggc gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg
    10081 accgcctccc gagatctgcg tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg
    10141 cgtggttaca tggttggtcc gaatggcccg gttaatctgc aaatcatcgt tagccaactg
    10201 tatgctgacg tgtcccaggg caacgtgcgc tacaacatcg cgaccaaagc ggatatcgcc
    10261 atcatcgcta ccgcgcagaa tggcaacaaa atgaccaaaa actatcgtgc cagctacaac
    10321 gttgaaggtg cgttccaggc ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg
    10381 accgatacca tcgctgatat gtctcaggac accagcatcc acgaattcat caagcagaac
    10441 gcgcgttaat ttctgccctc tggcccggtg caaaccgggc ctgtagacgc ccatgtccag
    10501 tcaatattta cgtatttttc aacagccgcg ttcagccata ttgctgatcc tgggttttgc
    10561 ttccgggcta ccgctcgccc tgacatccgg taccttacag gcctggatga cggtcgagaa
    10621 tattgatctc aaaaccattg gtttcttctc tctggtaggc caggcttacg tttttaaatt
    10681 cctctggtca ccgctgatgg accgctacac gcccccattt tttgggcggc ggcgcggttg
    10741 gctgctcgcc acgcaaatcc tgttattagt cgccattgcg gcgatgggtt ttctcgaacc
    10801 tggcacccaa ctccgctgga tggcggcact ggcggtggtg atcgcttttt gctcggcctc
    10861 ccaggatatt gtcttcgatg cgtggaaaac cgatgtgctc ccggcagaag aacgtggtgc
    10921 gggcgcggca atcagcgtgc tgggttaccg tttagggatg ctggtttccg gcggcttagc
    10981 cctgtggctg gcagataaat ggctgggctg gcagggcatg tactggttga tggcggcgct
    11041 gttgatccct tgtattatcg cgacgttgct tgcaccagaa ccgaccgaca ccattcctgt
    11101 gccaaaaacg ctggaacaag cggttgttgc acctctgcga gatttctttg gtcgcaataa
    11161 tgcctggctt attttgcttc ttatcgtgct gtataagctg ggcgacgcat tcgccatgag
    11221 cctgacaacc acgtttttga ttcgcggcgt cgggtttgat gcgggtgaag taggcgtggt
    11281 taacaaaacg cttggcttac tggcgaccat tgtgggcgca ttgtacggtg ggattttgat
    11341 gcagcgcctg tcgctgttcc gggcgctgct gattttcggc attttacaag gtgcgtctaa
    11401 cgctggttac tggctgctgt cgattactga taagcatctc tacagcatgg gcgcagccgt
    11461 ctttttcgaa aacctctgtg gcgggatggg cacatcagcc tttgtcgcgc tgttaatgac
    11521 gctgtgtaat aagtcatttt ccgctactca atttgccctg ctctcagcgc tttccgctgt
    11581 aggacgagtt tatgtcggcc ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac
    11641 attctatcta ttctccgtcg ccgctgccgt accggggctt attttgctgc tggtttgccg
    11701 ccagacgctt gaatatacac gagtaaatga caactttatc tcccgtaccg aatatccgac
    11761 aggttatgcc tttgccatgt ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt
    11821 actgctgttg acgatggacg cgctggattt gacgcacttc tctttcctgc ctgctctgct
    11881 ggaagtcggg gttttagtcg ccctttctgg cgtcgtgctt ggtggtttgc tggattatct
    11941 ggctctacga aaaacgcatc tgacgtaatc tgtaaatatt atttagctgt agtaatcact
    12001 cgccgtaatt attacggcta aataaacatc agtatcgctt aataatattt gttcttttgt
    12061 gcggcttagc gtttggaaat tattgacgca atttatgaat tctatttttt cttacacgat
    12121 tcagctaatg agtctttatt ttccccccac ccaattgtca ccataatgat aattatttgt
    12181 taaacaattg ttttattcca gattggttat accaattgcc cgtccaaact ccttctgcac
    12241 tcccctttcg ttataacgcc cttttgcaac agcttcttaa aatcaacttg atatgttttg
    12301 caacatatgt aacctgacag ccaaatccaa gtaacaggaa tttaatcatg cttacagtaa
    12361 tgtaacctcc ccgtaaaatg cccacacact ttaaacgcca ccagatcccg tggaattgag
    12421 gtcgttaaat gagactcagg aaatacaata aaagtttggg atggttgtca ttatttgcag
    12481 gcactgtatt gctcagtggc tgtaattctg cgctgttaga tcccaaagga cagattggtc
    12541 tggagcaacg ttcactgata ctgacggcat ttggcctgat gttgattgtc gttattcccg
    12601 caatcttgat ggctgttggt ttcgcctgga agtatcgtgc gagcaataaa gatgctaagt
    12661 acagcccgaa ctggtcacac tccaataaag tggaagctgt ggtctggacg gtacctatct
    12721 taatcatcat cttccttgcg gtactgacct ggaaaaccac tcacgctctt gagcctagca
    12781 agccgctggc acacgacgag aagcccatta ccatcgaagt ggtttccatg gactggaaat
    12841 ggttcttcat ctacccggaa cagggcattg caaccgtgaa tgaaatcgct ttcccggcga
    12901 acactccggt gtacttcaaa gtgacctcca actccgtgat gaactccttc ttcattccgc
    12961 gtctgggtag ccagatttat gccatggccg gtatgcagac tcgcctgcat ctgatcgcca
    13021 acgaacccgg tacttatgac ggtatctccg ccagctacag cgggccgggc ttctcaggca
    13081 tgaagttcaa agctattgca acaccggatc gcgccgaatt cgaccaatgg gtcgcaaaag
    13141 cgaaacagtc gccgaacacc atgtctgaca tggctgcgtt cgaaaaactg gccgcgccta
    13201 gcgaatacaa ccaggtggaa tatttctcca acgtgaaacc agacttgttt gccgatgtga
    13261 ttaacaagtt tatggctcac ggtaagagca tggacatgac ccagccagaa ggtgagcaca
    13321 gtgcacacga aggtatggaa ggcatggaca tgagccacgc ggaatccgcc cattaaaggg
    13381 gttgaggaag aataaagatg ttcggaaaat tatcacttga tgcagtcccg ttccatgaac
    13441 ctatcgtcat ggttacgatc gctggcatta ttttgggagg tctggcgctc gttggcctga
    13501 tcacttactt cggtaagtgg acctacctgt ggaaagagtg gctgacctcc gtcgaccata
    13561 aacgcctcgg tatcatgtat atcatcgtgg cgattgtgat gttgctgcgt ggttttgctg
    13621 acgccattat gatgcgtagc cagcaggctc ttgcctcggc gggcgaagcg ggcttcctgc
    13681 cacctcacca ctacgatcag atcttcaccg cgcacggcgt gattatgatc ttcttcgtgg
    13741 cgatgccttt cgttatcggt ctgatgaacc tggtggttcc gctgcagatc ggcgcgcgtg
    13801 acgttgcgtt cccgttcctc aacaacttaa gcttctggtt tactgttgtt ggtgtgattc
    13861 tggttaacgt ttctctcggc gtgggcgaat ttgcgcagac cggctggctg gcctatccac
    13921 cgctatcggg aatagagtac agtccgggag tcggtgtcga ttactggata tggagtctcc
    13981 agctatccgg tataggtacg acgcttaccg gtatcaactt cttcgttacc attctgaaga
    14041 tgcgcgcacc gggcatgacc atgttcaaga tgccagtatt tacctgggca tcactgtgcg
    14101 cgaacgtact gattattgct tccttcccaa ttctgacggt taccgtcgcg ttgttgaccc
    14161 tggatcgcta tctgggcacc catttcttta ccaacgatat gggtggcaac atgatgatgt
    14221 acatcaacct gatttgggcc tggggccacc cggaagttta catcctgatc ctgcctgttt
    14281 tcggtgtgtt ctccgaaatt gcggcaacct tctcgcgtaa acgtctgttt ggttatacct
    14341 cgctggtatg ggcaaccgtc tgtatcaccg tgctgtcgtt catcgtttgg ctgcaccact
    14401 tctttacgat gggtgcgggc gcgaacgtaa acgccttctt tggtatcacc acaatgatta
    14461 tcgccatccc gaccggggtg aagatcttca actggctgtt caccatgtat cagggccgca
    14521 tcgtgttcca ttctgcgatg ctgtggacca tcggttttat cgtcactttc tcggtgggcg
    14581 ggatgactgg cgtgctgctg gcagtaccgg gcgcggactt cgttctgcat aacagcctgt
    14641 tcctgattgc gcacttccat aacgttatca tcggcggcgt ggtcttcggc tgcttcgcag
    14701 ggatgaccta ctggtggcct aaagcgttcg gtttcaaact gaatgaaacc tggggtaaac
    14761 gcgcgttctg gttctggatc atcggcttct tcgttgcctt tatgccgctg tatgcgctgg
    14821 gcttcatggg catgacccgt cgtttgagcc agcagattga cccgcagttc cacaccatgc
    14881 tgatgattgc agctagcggt gcagtactga ttgcgctggg tattctctgc ctcgttattc
    14941 agatgtacgt ttctattcgc gaccgcgacc agaaccgtga cctgactggc gacccgtggg
    15001 gtggccgtac gctggagtgg gcaacctctt ccccgcctcc gttctataac tttgccgttg
    15061 tgccgcacgt tcacgagcgt gatgcattct gggaaatgaa agagaaaggc gaagcgtata
    15121 aaaagcctga ccactatgaa gaaattcata tgccgaaaaa cagcggtgca ggtatcgtca
    15181 ttgcagcttt ctccaccatc ttcggtttcg ccatgatctg gcatatctgg tggctggcga
    15241 ttgttggctt cgcaggcatg atcatcacct ggatcgtgaa aagcttcgac gaggacgtgg
    15301 attactacgt gccggtggca gaaatcgaaa aactggaaaa ccagcatttc gatgagatta
    15361 ctaaggcagg gctgaaaaat ggcaactgat actttgacgc acgcgactgc ccacgcgcac
    15421 gaacacgggc accacgatgc aggcggaacc aaaatcttcg gattttggat ctacctgatg
    15481 agcgactgca ttctgttctc aatcttgttt gctacctatg ccgttctggt gaacggcacc
    15541 gcaggcggcc cgacaggtaa ggacattttc gaactgccgt tcgttctggt tgaaactttc
    15601 ttgctgttgt tcagctccat cacctacggc atggcggcta tcgccatgta caaaaacaac
    15661 aaaagccagg ttatctcctg gctggcgttg acctggttgt ttggtgccgg atttatcggg
    15721 atggaaatct acgaattcca tcacctgatt gttaacggca tgggtccgga tcgcagcggc
    15781 ttcctgtcag cgttctttgc gctggtcggc acgcacggtc tgcacgtcac ttccggtctt
    15841 atctggatgg cggtgctgat ggtacaaatc gctcgtcgcg gcctgaccag cactaaccgt
    15901 acccgcatca tgtgcctgag cctgttctgg cacttcctgg atgtggtttg gatctgtgtg
    15961 ttcactgttg tttatctgat gggggcgatg taatgagtca ttctaccgat cacagcggcg
    16021 cgtcccatgg cagcgtaaaa acctacatga caggctttat cctgtcgatc attctgacgg
    16081 tgattccgtt ctggatggtg atgacaggag ctgcctctcc ggccgtaatt ctgggaacaa
    16141 tcctggcaat ggcagtggta caggttctgg tgcatctggt gtgcttcctg cacatgaata
    16201 ccaaatcaga tgaaggctgg aacatgacgg cgtttgtctt caccgtgcta atcatcgcta
    16261 tcctggttgt aggctccatc tggattatgt ggaacctcaa ctacaacatg atgatgcact
    16321 aagagcggcg gttatgatgt ttaagcaata cctgcaagta acgaaaccag gcatcatctt
    16381 tggcaacctg atctcggtga ttgggggatt cctgctggcc tcaaagggca gcattgatta
    16441 tcccctgttt atctacacgc tggttggggt gtcactggtt gtggcgtcgg gttgtgtgtt
    16501 taacaactac atcgacaggg atatcgacag aaagatggaa aggacgaaga atcgggtgct
    16561 ggttaaaggc ctgatctctc ctgctgtctc gctggtgtac gccaccttgc taggctttgc
    16621 tggctttatg ctgctgtggt ttggcgcgaa tccgctggcc tgctggctgg gggtgatggg
    16681 ctttgtggtt tatgtcggcg tttatagcct gtacatgaaa cgccactcgg tctacggcac
    16741 attgattggt tcgctctccg gcgctgcgcc gccggtgatt ggctactgtg cagtaaccgg
    16801 cgagttcgat agcggcgcag cgatcctgct ggctatcttc agcctgtggc agatgcctca
    16861 ctcctatgcc atcgccattt tccgctttaa ggattatcag gcggcaaaca ttccggtatt
    16921 gccagtggta aaaggcattt cggtggcgaa gaatcacatc acgctgtata tcatcgcctt
    16981 tgccgttgcc acgctgatgc tctctcttgg cggttacgct gggtataaat atctggtggt
    17041 cgccgcggcg gtgagcgtct ggtggttagg tatggccctg cgcggttata aagttgctga
    17101 tgacaggatc tgggcgcgca agctgttcgg cttctctatc atcgccatca cagccctctc
    17161 ggtgatgatg tccgttgatt ttatggtgcc ggactcgcat acgctgctgg ctgctgtttg
    17221 gtaacacaac ctctctatta aaaaggtgct acggcacctt ttttattgtc tgagatcaga
    17281 gatacggcta ataaccgtat gtcttgacag agtgagcaga tgaacataaa ttaatcatac
    17341 ggttgatagc cgtattatgt atgagatcga ctccctacga tttcattcag ttaatcaaag
    17401 aggtcataat gcctggaaaa acagccactc tacccgatgt cgataaaacg ctgaaaaatg
    17461 cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa acttcgtgaa gctgcgcaag
    17521 ccgttggggt agatttaagc gcttttattt tgagcgctgc tatggaacgc gccgaaagtg
    17581 tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag ctgggaactg atgaaccaac
    17641 tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc gttaatgaaa aggaaaaaca
    17701 gcgatggtcg acaagcatga agagattact ctgcccatag tcctctcctg taattatcag
    17761 tctgatatta cttatcctgg gcaaaaacag tttgattgcg gtaactctgt tatcgataaa
    17821 tttgtacgcg catcgctaaa gaaaagtgtg cgtaatagcg actgtgcggc taaagcactt
    17881 attgacagac aaagtggtga actgatcggc atctgtactt ttacggcata ttcgctggaa
    17941 aaacaacgcg tttctggcgt ccttcagggt tcacaacctt cagaaattgg tgttgtcaga
    18001 ttagtcatgt tgggggtagc acggaagtat caaaagcggg gctttggtca ggacctacta
    18061 tgtgattttt ttgaacatgt aaaaataatt caccaggcat taccaattaa aggggtttat
    18121 cttgatgctg accctgccgc cattaatttt tatgctcgtc tcggctttgt tcagctttca
    18181 gcgacaccaa atgcttttgg tgctgtacct atgtttttgg cgattcagca tattctcgcg
    18241 gcttagatta ttatcgccac ccgccagggc aagttttgcc ttagccgggg gctgagcagc
    18301 gttaccactt ttgttcaatt gcccccgctt tcagcaactc cacatccttg ccgttaatgg
    18361 ttgccgttgc ggcaacgatt gtggtcctta cagttgcaca agattttcgc tcaaattccg
    18421 gtttataaag aataagagac gaattactgg taaatgaacc aaataggtaa tcagtaaagc
    18481 taatattcag ccggtttaaa ccgaaaggcg cgaagccaac cgtttggtta atctccttac
    18541 cggatgggcg acgaatgctg aatgtcacgc gaagattggc cacttcgcct aatttattgg
    18601 tggtggcgaa gaggctatcc atgcaggaaa aacctttatc taaaaaatta tctcgcatcc
    18661 cagaacgggt aaccgtaatc acgggtaacg tatcgtcaac cgtttttctg tcaatcatgt
    18721 cttttcgaca agtagccaga aagtcttccg ggccaccata aatgtggaca cctttatcaa
    18781 gatattcctt cgctttctta caatctacct caacatactg accattggcg tacataatgc
    18841 cc
//
LOCUS       DAZRUF010000063        18832 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZRUF010000063 DAZRUF010000000
VERSION     DAZRUF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.063"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(75..2066)
                     /gene="tkt"
                     /locus_tag="ACHXOL_001752"
     CDS             complement(75..2066)
                     /gene="tkt"
                     /locus_tag="ACHXOL_001752"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR6808752.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2344..3102
                     /gene="loiP"
                     /locus_tag="ACHXOL_001753"
                     /gene_synonym="yggG"
     CDS             2344..3102
                     /gene="loiP"
                     /locus_tag="ACHXOL_001753"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR6808753.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3245..4165)
                     /gene="speB"
                     /locus_tag="ACHXOL_001754"
     CDS             complement(3245..4165)
                     /gene="speB"
                     /locus_tag="ACHXOL_001754"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR6808754.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4303..6279)
                     /gene="speA"
                     /locus_tag="ACHXOL_001755"
     CDS             complement(4303..6279)
                     /gene="speA"
                     /locus_tag="ACHXOL_001755"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR6808755.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(6288..6419)
                     /gene="yqgB"
                     /locus_tag="ACHXOL_001756"
     CDS             complement(6288..6419)
                     /gene="yqgB"
                     /locus_tag="ACHXOL_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR6808756.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(6513..6656)
                     /gene="yqgG"
                     /locus_tag="ACHXOL_001757"
     CDS             complement(6513..6656)
                     /gene="yqgG"
                     /locus_tag="ACHXOL_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR6808757.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(6767..7018)
                     /gene="yqgD"
                     /locus_tag="ACHXOL_001758"
     CDS             complement(6767..7018)
                     /gene="yqgD"
                     /locus_tag="ACHXOL_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR6808758.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            7074..8228
                     /gene="metK"
                     /locus_tag="ACHXOL_001759"
     CDS             7074..8228
                     /gene="metK"
                     /locus_tag="ACHXOL_001759"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR6808759.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            8664..10058
                     /gene="galP"
                     /locus_tag="ACHXOL_001760"
     CDS             8664..10058
                     /gene="galP"
                     /locus_tag="ACHXOL_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR6808760.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            10135..10632
                     /locus_tag="ACHXOL_001761"
     CDS             10135..10632
                     /locus_tag="ACHXOL_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR6808761.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            10727..11434
                     /gene="endA"
                     /locus_tag="ACHXOL_001762"
     CDS             10727..11434
                     /gene="endA"
                     /locus_tag="ACHXOL_001762"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR6808762.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            11514..12245
                     /gene="rsmE"
                     /locus_tag="ACHXOL_001763"
     CDS             11514..12245
                     /gene="rsmE"
                     /locus_tag="ACHXOL_001763"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR6808763.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGAIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            12258..13208
                     /gene="gshB"
                     /locus_tag="ACHXOL_001764"
     CDS             12258..13208
                     /gene="gshB"
                     /locus_tag="ACHXOL_001764"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR6808764.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            13317..13880
                     /locus_tag="ACHXOL_001765"
     CDS             13317..13880
                     /locus_tag="ACHXOL_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR6808765.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            13880..14296
                     /gene="ruvX"
                     /locus_tag="ACHXOL_001766"
     CDS             13880..14296
                     /gene="ruvX"
                     /locus_tag="ACHXOL_001766"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR6808766.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(14471..15451)
                     /locus_tag="ACHXOL_001767"
     CDS             complement(14471..15451)
                     /locus_tag="ACHXOL_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR6808767.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            15469..16173
                     /gene="yggS"
                     /locus_tag="ACHXOL_001768"
     CDS             15469..16173
                     /gene="yggS"
                     /locus_tag="ACHXOL_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR6808768.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16191..16757
                     /gene="yggT"
                     /locus_tag="ACHXOL_001769"
     CDS             16191..16757
                     /gene="yggT"
                     /locus_tag="ACHXOL_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR6808769.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            16754..17044
                     /gene="yggU"
                     /locus_tag="ACHXOL_001770"
     CDS             16754..17044
                     /gene="yggU"
                     /locus_tag="ACHXOL_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR6808770.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            17052..17645
                     /locus_tag="ACHXOL_001771"
     CDS             17052..17645
                     /locus_tag="ACHXOL_001771"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR6808771.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            17638..18774
                     /gene="hemW"
                     /locus_tag="ACHXOL_001772"
     CDS             17638..18774
                     /gene="hemW"
                     /locus_tag="ACHXOL_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR6808772.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         4479 a         5020 c         4924 g         4409 t
ORIGIN      
        1 ttgcttgtcc gcaaacggac atatcaaggt aataaaaaag gtcgccgaag cgaccttttt
       61 acccgaaatg ctaattacag cagttctttt gctttcgcaa cgacgttgtc gacggtgaag
      121 ccgaactctt cgaacagcaa ctctgccgga gctgactcac cgaaggtggt cataccgatg
      181 atagcgccat tcaggccaac atacttgtac cagtagtcag caatacccgc ttctaccgca
      241 acgcgtgcag taaccgcttt cggcagtacg gattcacggt aagccgcatc ctgcttgtcg
      301 aatgcatcgg tagacggcat ggaaaccacg cgcgccttca cgccttcggc agtcagtttt
      361 tcgtaggcgg caacagccag ttcaacttcc gaaccggtcg cgatgaagat cagttccggc
      421 tgaccagcac agtctttcag cacataacca ccgcgcgcga tgtttgccag ttgctcttca
      481 gtacgttcct gctgcgccag attctgacgg gagaggataa gcgcggtcgg accgtcctga
      541 cgctcaacac cgtatttcca cgcgaccgcg gattcaacct ggtcacacgg acgccatgta
      601 gacatgttcg gggtcacgcg cagagaagcg acctgctcaa ccggctggtg agtcgggccg
      661 tcttcgccca gaccgatgga gtcgtgggtg taaaccatca cctgacgctg tttcatcagc
      721 gcagccatac gtacagcgtt acgtgcgtat tcgacaaaca tcaggaaggt ggaggtgtac
      781 ggcaggaagc caccgtgcag ggagataccg ttagcaatcg cggtcatacc gaactcgcga
      841 acaccgtagt ggatgtagtt acccgcagca tcttcgttga ttgctttaga accagaccac
      901 agggtcaggt tagacggtgc caggtcagcg gagccgccga ggaattccgg caacagcggg
      961 ccgaacgctt caatagcatt ctgagacgct ttacggctgg cgattttcgc cggattagcc
     1021 tgcagtttag cgataaactc tttcgctttg gcgtcgaagt cagacggcat ttcgcctttc
     1081 atacggcggg taaattcagc cgcttcctgc ggataagctt tcgcgtaagc cgcaaacttc
     1141 tcattccatg cagattcttt cgcctggcct gcttctttcg catcccactg cgcatagatt
     1201 tcagacggga tttcgaacgg tgcgtatttc cagcccagct gttcgcgggt cagagcgatt
     1261 tctgcatcac ccagcggcgc accgtgggag tcgtgagtac cggctttgtt cggggaaccg
     1321 aaaccgatga tggttttgca catcagcagg gacggtttgt cagtcactgc gcgcgcttct
     1381 tctactgcgc gtttgatgga tgctgcgtca tgaccgtcga tgtcgcgaat aacgtgccag
     1441 ccgtaagctt cgaaacgcat tgcggtgtcg tcggtgaacc agccttcaac gtgaccatca
     1501 atagagatac cgttgtcatc gtagaacgca atcagtttac ccagcttcag cgtacccgcc
     1561 agagagcaaa cttcgtggga gatgccttcc atcatgcagc cgtcgcccat gaaggcgtag
     1621 gtgtagtggt cgacaatgtc gtggcccgga cggttaaact gcgccgccag cgttttttct
     1681 gcaatcgcca taccgactgc gttggcaata ccctgaccca gcggaccggt ggtggtttcc
     1741 acaccagcgg tataacctac ttccgggtgg cccggagttt tagagtgcag ctgacggaag
     1801 tttttcagtt cttccatcgg cagatcgtaa ccggtgaggt gcagcaggct gtagatcagc
     1861 atggagccgt ggccgttgga cagcacgaag cggtcacggt cagcccagga cggattctgc
     1921 gggttgtgtt tcaggaaatc acgccacagg acttcggcaa tgtcagccat acccataggg
     1981 gcacccgggt gaccggattt ggctttctgt actgcgtcca tgctcagcgc acgaatagca
     2041 ttggcaagct ctttacgtga ggacattttg actccagatc ggatgatgaa gggcacgccc
     2101 ttaacgactt gacgacagcg cgttttgggc tacgccggaa aatttgccaa caatttaccg
     2161 caagccgcgc gtcatgtaca tggaacatcc ttttgccgct tcagaaatct ctggatcatg
     2221 ctcgcatgtt gcgcaatcta ctcgcccgtc cgctgcgctt ttccttatac tgagactgag
     2281 cgtcgattca cctgcaaacg gcgcattttt agaataatcc tgaccttgtg cggaagagaa
     2341 aacatgaaaa ttcgcgcctt attggtagca atgagcgtgg caacggtact gactggttgc
     2401 cagaatatgg actccaacgg actgctctca tcaggagcgg aagcttttca ggcttacagt
     2461 ttgagtgatg cgcaggtgaa aaccctgagc gatcaggcat gtcaggagat ggacagcaag
     2521 gcgacgattg cgccagccaa tagcgaatac gctaaacgtc tgacaactat tgccaatgcg
     2581 ctaggcaaca atatcaacgg tcagccggtg aattacaaag tgtatatggc gaaggatgtg
     2641 aacgcctttg caatggctaa cggctgtatc cgcgtctata gcgggctgat ggatatgatg
     2701 acagataacg aagtcgaagc ggtgatcggt cacgagatgg ggcacgtggc gttaggccat
     2761 gtgaagaaag ggatgcaggt ggcgcttggt acaaatgccg tgcgagtagc tgcggcctct
     2821 gcgggcggga ttgtcggcag tttatctcaa tcacaacttg gtgatctggg cgagaaatta
     2881 gtcaattcgc aattctccca gcgccaggaa gcagaagccg atgattattc ttacgatctt
     2941 ctgcgccaac gcggcatcag cccggcaggt cttgccacca gctttgaaaa actggcgaaa
     3001 ctggaagaag gccgccaaag ctcaatgttt gacgaccatc cggcatccgc cgaacgcgcc
     3061 cagcatattc gcgatcgcat gagcgcggat gggattaagt aaagcctggt ggtgtcgaac
     3121 gtaggtcaga taaggcgttc acgccgcatc tgacatcaat ggcacatttg ttaccctgtg
     3181 cgcattgccg gatgcgatgc tgactgcatc ttatccggcc tacgggtggc atcgcatctg
     3241 gtgcttactc gccctttttc gccgcttgga tatacagcat ctccagcgcc agcgtcgccg
     3301 cagccagagc ggtgatttcc gactgatcgt atgccggagc cacttccact acgtccatcc
     3361 caacaatgtt gagatctttc aggccgcgta ccagtttaat agcgcgatcg gaggtcaggc
     3421 cgccaatcac tggcgtaccg gtgcctggtg caaaagcagg atccaggcag tcgatatcaa
     3481 aagtcaggta aaccggcata tcacccacaa tctgtttcac ttgggcgata acgtcatcca
     3541 cgctgcgatc gttcacctgg caggcgtcca gcacggtaaa gccgttgtct ttatcaaact
     3601 cggtacgaat accaatctgc acggaatgat tcgggtcgat cagaccttct ttcggcgcgg
     3661 tatagaacat agtgccgtgg tcaaattcac aaccgttcgc ataggtatcg gtgtgggcgt
     3721 caaagtgtac cagcgccatt ttgccgaaat gcttcgcatg agcacgcagc agcggcagcg
     3781 taacaaagtg gtcaccaccg aaggtgagca tacgcttacc ggcagccagc agcttctcgg
     3841 cgtgcgcctg cagcttttcg ctcatctcac gggcatcgcc aaaggcatat accagatcgc
     3901 cgcagtccac gacgttcaga cgctcacgca tgtcgaaatt ccacgggaag cggttgtgtt
     3961 cccaggccag attcgtcgaa acctgacgga tcgctgccgg accgtggcga ccacccgcac
     4021 gaccagaagt ggccatatcg aacggcacgc cagtaatcac ccagtctgca tcgctgtcat
     4081 acggctggaa gttcatcggc aggcgtaaaa aaccaaaggc attggaaacc agtgagttat
     4141 cgtattgatg acctaaggtg ctcatggatg gactcctatt acaaagtcga tataaaaaaa
     4201 cccttccgcg tcgttaggcc cgacgaggaa gggttggatt tgtcacaata aattgtggcg
     4261 gattatcacc gctaaattaa gcggattcaa gtaacacagg acttactcat cttcaagata
     4321 agtataaccg tacaaacctg cctcgaactc ttcaaggaac tgttgttgca gttcagcatc
     4381 aagatcggtt ttcttcactt gatcgcggaa ctgggttaac agcgttttcg gatcgagctg
     4441 tacatattgc agcatgtccg ccacggtatc gccttcgtca gacagttcta cttctacgct
     4501 accgtcaggg aacacgaaca cgtcaaccgc ttcggtatca ccgaacaggt tgtgcatatt
     4561 gccaaggatc tcctgatatg cgccgaccat aaagaaaccg agcatcggcg gattctctgg
     4621 atcgtactcc ggcattggca ttgtcgtggc aataccatca ccatcaatat agtggtcgat
     4681 agcaccgtca gagtcacagg taatatccag cagcacagcg cgacgttccg gcacttgatc
     4741 cagcccttcc agcggcagaa ccgggaacaa ctggtcgatc ccccatgcgt ccggcattga
     4801 ctggaacagc gagaaattga cgtacatttt gtccgccata cgttcctgca gttcgtcgat
     4861 tatcggacga tgcgcacggt tttgcggatc cagttgcttc tgcacttcat ggcacatgct
     4921 cagatagagt tgttctgccc atgcacgttc ttgcaggcta aaggtgccgg aagagtagcc
     4981 gatatgaatg tcgtgcagat ccatctggct gtcgtgtaac cattcacgca aagaacggcg
     5041 agttcccggt tcgtgcattt cctgccaggt ttcccacatg ctttgcagcg cgcgcggcgc
     5101 atcttctgcg ggcgcggtcg gcaccgtata ttcgttacgt tccacgccga tgatattcga
     5161 caccagcacg gtatggtgcg cagtcaccgc acggcccgat tcggtgatta ccgtcggatg
     5221 cggcagacca ttttcttcac acgcatcgcc aatcgcccag atgatgttgt tggcgtattc
     5281 attgaggcca tagtttacgg agcagtcaga ctgcgaacgg gtgccttcat agtccacgcc
     5341 cagaccgccg ccgacgtcga agcactgaat attgacgccc agcttgtgca gttccacata
     5401 gaaacgcgcg gattcacgaa cgcccgtggc gatatcgcga atattcgcca tctgcgaacc
     5461 gaggtggaag tgcagtagtt gcaggctgtc gagacgcccg gcttcacgca gggtttcaac
     5521 cagttgcagt acctgagtcg cagccaggcc gaacttcgat ttttccccgc cggaggactg
     5581 ccatttaccc gagccctgcg aagccagacg tgcacgcacg cccagacgag gaacgacatt
     5641 cagacgttct gcttcatcca gcacaatggc gatttctgac atcttctcaa tgaccagata
     5701 gaccttgtgc cccatcttct cgccaattaa tgccaggcgg atatattcgc ggtctttata
     5761 accgttgcag acgatgacgc tacgggtcat gccagcatgt gccagtactg ccatcaactc
     5821 ggctttggaa ccggcttcca gacccagcgg ttcgcccgaa tgaatcaggg actcaatcac
     5881 gcggcggtgc tggttaactt tgatcggata aacaaggaag taatcgccgt tatagccgta
     5941 ggattccctc gcacgtttga acgcggcgtt aatggaacgc aaacggtgct gcaggatctg
     6001 tgggaaacag aacagtgcag gcagacgctg accctgtgct tcacgagttt tcactaactg
     6061 cgcgagatcg acgcgagctt ccgggacgtc cgggtccggg cacacgctga tgtggcccag
     6121 ctcgttaacg tcatagtagt tattgcccca ccaggcaata ttgtaagtac gcagcatctt
     6181 gctggcttcc tgggagctca ttgcaacctc ctgcatggag cgtagtacac cgtgttcgcc
     6241 cgctgacgaa ggcaaaccca tagacatgtc gtcagacata gcgaacctca aattatttta
     6301 ttaagtgtaa aacagttaac gactatcgca gcctggaact gcgataacaa cccataagca
     6361 catggatttt ccagcagtga atgctgacgc tccaactgcg caaccggttt ctttttcata
     6421 acattattaa gcacataacc gaacgtaagt gtgacagttc ggcgaaacca cgagaaaact
     6481 cttgttttta caagagcgcc cttgttcagt cctcagtaac tgtaaccagc tcttgaatcc
     6541 tgagaagcgc cgagatgggt ataacatcgg caggtatgca aagcagagat gcagagtgcg
     6601 gggaacgaat cttcaccaga acggtgagac aggttaagca gcagacaacg gttcattatt
     6661 tcgtatcacc tccacggccg cctgttaaga cgaacccaca agccaaaact ctgatttcaa
     6721 cccggctgga agtggcaaca cgaaagaaac gtcgtgtgct ttttatttaa gccgcgcgcc
     6781 gcgttttata ccccacaatg gcagaaaatt gcaaaagata aatacgcaga atgccggcat
     6841 tgtcaggaaa aatttccagc cacgttttta acagaatgag acacgattca aaaaaaagtg
     6901 gaaatcgggt gaagaattga cctaaaatag ccatccagat gttaatccat ccataccgat
     6961 taacactcag actgccagtg tttttaacct gcagagtcgt ggtaggatcc gctaccacag
     7021 aaaatccaca caacagtttg agctaaccaa attctcttta ggtgatatta aatatggcaa
     7081 aacacctttt tacgtccgag tccgtctctg aagggcatcc tgacaaaatt gctgaccaaa
     7141 tctctgatgc cgttttagac gcgatcctcg aacaggatcc gaaagcacgc gttgcttgcg
     7201 aaacctacgt aaaaaccggc atggttttag ttggcggcga aatcaccacc agcgcctggg
     7261 tagacatcga agagatcacc cgtaacaccg ttcgcgaaat tggctatgtg cattccgaca
     7321 tgggctttga cgctaactcc tgtgcggttc tgagcgctat cggcaaacag tctcctgaca
     7381 tcaaccaggg cgttgaccgt gccgatccgc tggaacaggg cgcgggtgac cagggtctga
     7441 tgtttggcta cgcaactaat gaaaccgacg tgctgatgcc agcacctatc acctatgcac
     7501 accgtctggt acagcgtcag gctgaagtgc gtaaaaacgg cactctgccg tggctgcgcc
     7561 cggacgcgaa aagccaggtg actttccagt atgacgacgg caaaatcgtt ggtatcgatg
     7621 ctgtcgtgct ttccactcag cactctgaag agatcgacca gaaatcgctg caagaagcgg
     7681 taatggaaga gatcatcaag ccgattctgc ccgctgaatg gctgacttct gccaccaaat
     7741 tcttcatcaa cccgaccggt cgttttgtta tcggtggccc gatgggtgac tgcggtctga
     7801 ctggtcgtaa aattatcgtt gatacctacg gcggcatggc acgtcacggt ggcggagcat
     7861 tctctggtaa agatccatca aaagtggacc gttccgcagc ctacgcagca cgttatgtcg
     7921 cgaaaaacat cgttgctgct ggcctggccg atcgttgtga aattcaggtt tcctacgcaa
     7981 tcggcgtggc tgaaccgacc tccattatgg tagaaacttt cggtactgag aaagtgcctt
     8041 ctgaacaact gactctgctg gtacgtgagt tcttcgacct gcgcccatac ggtctgattc
     8101 agatgctgga tctgctgcac ccgatctaca aagaaaccgc agcatacggt cactttggtc
     8161 gtgaacattt cccgtgggaa aaaaccgaca aagcgcagct gctgcgcgat gctgccggtc
     8221 tgaagtaatc tttcttcacc tgctttcaaa ggccagcctc gcgctggcct ttttctttta
     8281 cctcaccgcg gtggataggc attcacgccg catccggcaa aaagctgccc gcacaataac
     8341 atcattcttc ctcaacacgt tttatcgcaa attatcatca ctactgaaac cgattacacc
     8401 aataacacca gccaaatatt tgtaatattt tcatattatt attcagtttt cacagttgtt
     8461 acatttcttt tcagtaaagt cttaattgca gataacagcg tttaatctat gatgatataa
     8521 ctcaattatt ttcatgcact taaaccataa ctaagataaa tgttagtgta agcgattaca
     8581 ctgatgtgat ttgcttcaca tctttttacg tcgtactcac ctatcttaat tcacaataaa
     8641 aaataaccat attggagggc atcatgcctg acgctaaaaa acaggggcgg tcaaacaagg
     8701 caatgacgtt tttcgtctgc ttccttgccg ctctggcggg attactcttt ggcctggata
     8761 tcggtgtaat tgctggcgca ctgccgttta ttgcagatga attccagatt acttcgcaca
     8821 cgcaagaatg ggtcgtaagc tccatgatgt tcggtgcggc agtcggtgcg gtgggcagcg
     8881 gctggctctc ctttaaactc gggcgcaaaa agagcctgat gatcggcgca attttgtttg
     8941 ttgccggttc gctgttctct gcggctgcgc caaacgttga agtactgatt ctttcccgcg
     9001 ttctactggg gctggcggtg ggtgtggcct cttataccgc accgctgtac ctctctgaaa
     9061 ttgcgccgga aaaaattcgt ggcagtatga tctcgatgta tcagttgatg atcactatcg
     9121 ggatcctcgg tgcttatctt tctgataccg ccttcagcta caccggtgca tggcgctgga
     9181 tgctgggtgt gattatcatc ccggcaattt tgctgctgat tggtgtcttc ttcctgccag
     9241 acagcccacg ttggtttgcc gccaaacgcc gttttgttga tgccgaacgc gtgctgctac
     9301 gcctgcgtga caccagcgcg gaagcgaaac gcgaactgga tgaaatccgt gaaagtttgc
     9361 aggttaaaca gagtggctgg gcgctgttta aagagaacag caacttccgc cgcgcggtgt
     9421 tccttggcgt actgttgcag gtaatgcagc aattcaccgg gatgaacgtc atcatgtatt
     9481 acgcgccgaa aatcttcgaa ctggcgggtt ataccaacac cacagagcaa atgtggggga
     9541 cagtgattgt cggcctgacc aacgtacttg ccacctttat cgcaatcggc cttgttgacc
     9601 gctggggacg aaaaccaacg ctaacgctgg gcttcctggt gatggctgct ggcatgggcg
     9661 tactcggtac aatgatgcat atcggtatcc actctccgtc ggcgcagtat ttcgccatcg
     9721 ccatgctgct gatgtttatt gtcggttttg ccatgagtgc cggtccgctg atttgggtac
     9781 tgtgctccga aattcagccg ctgaaaggcc gcgattttgg catcacctgc tccactgcca
     9841 ccaactggat tgccaacatg atcgttggcg caacgttcct gaccatgctc aacacgctgg
     9901 gtaacgccaa caccttctgg gtgtatgcgg ctctgaacgt actgtttatc ctgctgacat
     9961 tgtggctggt accggaaacc aaacacgttt cgctggaaca tattgaacgt aacctgatga
    10021 aaggtcgtaa actgcgcgaa atcggcgctc acgattaatc tcctcaggct tcctcccgtc
    10081 gcggaggaag ccgcctcttg cagtcatctt ttcttcgctc tatcctctgc cgctatgaaa
    10141 acatcccgtc tccctatcgc catccaacag gccgttatgc gcggcctgcg ggaaaaactc
    10201 gcccaggcca acctgaagct agggcgtaac tacccggagc caaaactctc ttacacccag
    10261 cgcggaacct ccgccggaac agcctggctg gaaagctatg aaatccgcct caatcccgtt
    10321 ttgctgttgg aaaacagtga agcttttatt gaagaagtgg taccgcacga actggcacat
    10381 ttgctggtat ggaaacattt cggccgcgta gcgccacatg gcaaagagtg gaagtggatg
    10441 atggaaagcg tgctgggtgt tcccgcccgt cgtacgcatc agttcgaact gcaatccgtg
    10501 cgtcgcaaca ccttccccta ccgctgcaag tgccaggagc atcagcttac cgtacgccgc
    10561 cataatcgcg tagttcgtgg cgaggccgtc tatcgctgtg ttcactgcgg tgaacagctg
    10621 gttgcgaaat aaccatctga actatcagga actttcctga tctggctgat tgcataccaa
    10681 aacaactttc gctacgttgc tggctcgttt taacacggag taagtgatgt accgttattt
    10741 gtctattgct gcggtggtac tgagcgcagc attttccggc ccggcgttgg ccgaaggtat
    10801 caatagtttt tctcaggcga aagccgcggc ggtaaaagtc cacgctgacg cgcccggtac
    10861 gttttattgc ggatgtaaaa ttaactggca gggcaaaaaa ggcgttgttg atctgcaatc
    10921 gtgcggctat caggtgcgca aaaatgaaaa ccgagccagc cgcgtagagt gggaacacgt
    10981 cgttcccgcc tggcagttcg gtcaccagcg ccagtgctgg caggacggtg gacgtaaaaa
    11041 ctgcgctaaa gatccggtct atcgcaagat ggaaagcgat atgcataacc tgcagccgtc
    11101 agtcggtgag gtgaatggcg atcgcggcaa ctttatgtac agccagtgga atggcggcga
    11161 aggccagtac ggtcaatgcg ccatgaaggt cgatttcaaa gaaaaagctg ccgaaccacc
    11221 agcgcgtgca cgcggtgcca ttgcgcgcac ctacttctat atgcgcgacc aatacaacct
    11281 gacactctct cgccagcaaa cgcagctgtt caacgcatgg aacaagatgt atccggttac
    11341 cgactgggag tgcgagcgcg atgaacgcat cgcgaaggtg caaggcaatc ataacccgta
    11401 tgtgcaacgc gcttgccagg cgcgaaagag ctaacctaca ctagcgggat tctttttgtt
    11461 aacccctacc ccacgcgtac aaccgcgtgg ggagacgacg cggattttta actatgcgta
    11521 tcccccgcat ttatcatcct gaaccactga ccagccattc tcacatcgcg ctttgcgaag
    11581 atgccgccaa ccatatcggg cgcgtactgc gcatggggcc ggggcaggcg ttgcaattgt
    11641 ttgacggtag caaccaggtc tttgacgccg aaattaccag cgccagcaaa aaaagcgtgg
    11701 aagtgaaggt gctggaaggc gcgatcgacg atcgcgaatc tccgctgcat attcacctcg
    11761 gtcaggtgat gtcgcgtggt gaaaaaatgg aatttacgat ccagaaatcg atcgaactcg
    11821 gtgtaagcct cattacgcca cttttttctg agcgctgcgg cgttaaactg gatagtgaac
    11881 gtctgaacaa gaagctccag cagtggcaga agattgcaat tgctgcctgt gagcagtgtg
    11941 gtcgtaaccg ggtgccggaa atccgtccag cgatggatct ggaagcctgg tgtgcagagc
    12001 aggatgaagg actgaaactg aatcttcacc cgcgcgccag taacagcatc aatacgttgc
    12061 cgttaccggt tgaacgcgtc cgcctgctga ttggcccgga aggcggttta tcggcagatg
    12121 aaattgccat gactgcccgc tatcaattta ctgatatcct gttgggacct cgcgttttgc
    12181 gtacagagac aactgcgctc accgccatta ccgcgctaca agtacgattt ggcgatttgg
    12241 gctaacggag aagaataatg atcaagctcg gcatcgtgat ggaccccatc gcaaacatca
    12301 acatcaagaa agattccagt ttcgctatgt tgctggaagc acagcgtcgt ggttacgaac
    12361 ttcactatat ggagatgggc gatctgtatc tgatcaatgg cgaagcccgc gcccataccc
    12421 gcacgctgaa cgtgaagcag aactacgaag agtggttttc gttcgtcggt gaacaggatc
    12481 tgccgctggc cgatctcgat gtgatcctga tgcgtaaaga cccgccgttt gataccgagt
    12541 ttatctacgc gacctatatt ctggaacgtg ccgaagagaa agggacgctg atcgttaaca
    12601 agccgcagag cctgcgcgac tgtaacgaga aactgtttac cgcctggttc tctgacttaa
    12661 cgccagaaac gctggttacg cgcaataaag cgcagctaaa agcgttctgg gagaaacaca
    12721 gcgacatcat tcttaagccg ctggacggta tgggcggcgc gtcgattttc cgcgtgaaag
    12781 aaggcgatcc aaacctcggc gtgattgccg aaaccctgac tgagcatggc actcgctact
    12841 gcatggcaca aaattacctg ccagccatta aagatggcga caaacgcgtg ctggtggtgg
    12901 atggcgagcc ggtaccgtac tgcctggcgc gtattccgca ggggggcgaa acccgtggca
    12961 atctggctgc cggtggtcgc ggtgaacctc gtccgctgac ggaaagtgac tggaaaatcg
    13021 cccgtcagat cgggccgacg ctgaaagaaa aagggctgat ttttgttggt ctggatatca
    13081 tcggcgaccg tctgactgaa attaacgtca ccagcccaac ctgtattcgt gagattgaag
    13141 cagagtttcc ggtgtcgatc accggaatgt taatggatgc catcgaagca cgtttacagc
    13201 agcagtaacc caccttagcg agaaggatct cgttgagact ctgagtgaca gcgcccttct
    13261 ttccacgcat actgggcgct gttgcttttt tgaaccagga aacagaacct ctgacaatga
    13321 atttacagca tcactttctt attgccatgc ctgctctcca ggatccgatt ttccgtcgtt
    13381 ccgtggtcta catttgcgaa cataatacca atggtgcaat ggggatcatc gtcaacaagc
    13441 cgctggaaaa tctcaaaatt gaagggattc tggaaaagct gaagatcacg ccggagccgc
    13501 gtgatgaatc aatccgtctg gataaaccgg ttatgctcgg cggtccgctg gctgaagatc
    13561 gcgggtttat tttgcatact ccgccctcca attttgcttc cagcattcgc atttcagaca
    13621 acacagtgat gaccacttcc cgcgatgtgc tggaaacgct cggcaccgat aaacaaccgt
    13681 ctgacgtatt ggtggctcta ggttatgcct cctgggaaaa aggtcaactg gaacaagaaa
    13741 ttctcgataa cgcgtggcta acggccccgg cggatctgaa tattctgttc aaaacgccga
    13801 ttgccgaccg ctggcgcgag gcggcaaaac tgattggtgt ggatattctc accatgcctg
    13861 gtgtggcagg gcacgcctga tgagtggaac cttactcgcc ttcgacttcg gcaccaaaag
    13921 cattggcgta gcggtcggcc aacgcattac cggcactgct cgccctttgc ctgcaattaa
    13981 agcacaggac ggtacgccgg actggaacct catcgaacgt ttactgaaag agtggcagcc
    14041 ggacgaaatc atcgtcggtt tgccgctgaa tatggacggc accgagcaac cattgactgc
    14101 cagagcgcgt aaatttgcca accgtattca tggtcgtttc ggtgttgaag taaagctcca
    14161 tgacgaacgt cttagcaccg tggaagcccg ttccggtctg tttgaacagg gcggctatcg
    14221 ggcgctcaac aaaggcaaag tcgactctgc ctctgcggtt attattctcg aaagctattt
    14281 cgagcaggga tattaaggcg atttaaaagc ctggcggata gtcgttaccg ccatcttgcc
    14341 ggatgcggcg tgaacgccta tccggcctac atgtggcagc gattgtaggt ctgataagac
    14401 gcgcaaacgt ctcatcagac gtttattgcc tttgtaggcc cgataagctt gcacatcggg
    14461 catggcaacg tcacaaacgc ccttccccca ctcgctgctg aaaactctgc tgaaacgtta
    14521 tcatccccac ctgctgcccg gtttgaataa catgcggtaa ctggtgggtt ttcccttcgc
    14581 gaatcaaatt ccccaccgcg ggagtgttaa tcaataattc aaacagcgcc acgcgtcctt
    14641 cctgtttatc cacttccagc ttttgtgaca acacggcccg taaactacct gccagttgat
    14701 tacgtacggg atctttttcc tgcgccggaa atgaatccac cagtcgctca actgcctgcg
    14761 cggcaccacg cgtatgtaat gttgccagca ccaaatgccc ggtttctgcc gccgtcagtg
    14821 ccagacggat tgtctcgctg tcacgcagct ctccgagcaa aatcacatca ggatcttccc
    14881 gcaatgcggc ccgcaatccc gatgcgaacg tcatacagtg caaaccaatt tcccgcttct
    14941 ggatcaaaca tcgctggctg gcatagatat attcaacagg atcttccagc gtcagaatat
    15001 gcgcatcggc atgttgattg agatagccaa ccatcgccgc cagcgtggta gatttgctgc
    15061 tccccgtcgc ccccgtcacc agaatcaggc cattctcact cttgagtaat tccggcaata
    15121 ccgttggtgc gccaagctgt tcgagctgcg ggcagtgcga aggtaacagc cgtaacgcca
    15181 gcgaaatgcc atgccgttgc gcaaatgcgc tgccacgcaa tcgctggttt tccgccagcg
    15241 acacggcaaa atccagctgg ccattttcca gcaatattgc ccgctgatcg tcatccagcc
    15301 actcccgcag tagctcttcg acgtccggcg cctcaaacgg cgcagcttcc attagcccgc
    15361 gaatgcgcca tcgtgcgggc caggcgctgc acaggtgtag atccgagacg ttatgcttta
    15421 cactaagggc cacaatttct tccatattca tactaagatc ctcggaaaat gaacgatatt
    15481 gcgcataacc tggcacaggt ccgggacaaa atctcagcgg ctgcaacgcg ttgcggccgt
    15541 tctccagaag aaattacgct gcttgcagtc agtaaaacaa aacctgcgag cgccatcgca
    15601 gaagccattg atgccgggca gcgtcaattt ggtgaaaact acgttcagga aggggtagat
    15661 aaaattcgcc actttcagga actgggcgta acaggattag aatggcattt tattggcccg
    15721 ttgcagtcta ataaaagccg cctggtggca gagcatttcg actggtgtca taccatcgac
    15781 cgtttgcgca ttgctacccg cctcaacgat cagcgcccgg cagaacttcc ccctcttaac
    15841 gtactgattc aaattaatat tagtgatgaa aacagtaagt ccgggattca actggcagaa
    15901 ctggacgagc tggcagcggc ggtcgctgaa ctaccgcgtt tacgtctgcg cgggctgatg
    15961 gcaatccctg cgcctgagtc agaatatgta aggcagtttg aagttgcacg ccaaatggct
    16021 gtagcatttg ccggactgaa aacgcgctac ccgcatatcg acacgctctc tctgggaatg
    16081 tcggacgata tggaagccgc cattgcggca ggtagcacga tggttcgtat cggcactgca
    16141 atttttggtg cgcgtgatta ctctaaaaaa taaggaatta aaggaacgcc atgaatacgt
    16201 tgactttcct gctttcaacg gtcattgagc tgtataccat ggtgctgtta ttacgcatct
    16261 ggatgcagtg ggctcattgt gatttttaca accccttctc acagtttgta gtgaaggtaa
    16321 cgcagccaat tatcgggcca ctgcgccgcg ttattccggc aatgggcccc attgacagcg
    16381 cctcgctgct ggttgcctat attctcagtt ttatcaaagc catcgtgctg tttaaagtgg
    16441 tgaccttcct gccaatcatc tggattgctg gtttactgat tctgctgaaa accatcggcc
    16501 tgctgatttt ctgggtcctg ctggtgatgg cgattatgag ctgggtaagc cagggtcgta
    16561 gtccgattga atacgtgctg attcagctgg ccgatccgct gctgcgcccg attcgccgcc
    16621 tgctaccggc aatgggtggg attgatttct cgccgatgat cctcgttctg ctgctgtatg
    16681 tcatcaatat gggtgtcgca gaagtattac aggcaaccgg aaacatgctg ctgccggggc
    16741 tgtggatggc gttatgagtg ccgtaacagt taatgatgac ggtctggttt tacggctcta
    16801 tattcagccg aaagccagcc gtgattctat tgtcggttta catggcgacg aagttaaagt
    16861 cgccattacc gcgccgccgg ttgacggcca gggcaacagt catctggtga agtttctcgg
    16921 taagcaattc cgggttgcca aaagccaggt ggtgattgaa aaaggcgaac ttggccgcca
    16981 caaacaaatt aaaatcatta atccgcaaca aatcccgcca gaaatcgcgg cgttaattaa
    17041 ttaggtatcc tatgcaaaaa gttgtcctcg caaccggcaa tgccggtaaa gtgcgtgagc
    17101 tggcgtcgct gcttagcgac ttcggtcttg atatcgtggc ccaaacagac ctcggcgtag
    17161 attccgctga agaaaccggc ctgaccttta tcgaaaacgc gattctgaaa gcgcgctatg
    17221 cggcaaaagt gaccggttta ccggcaattg ccgacgactc tggtctggcg gtagatgcgc
    17281 tgggtggcgc gccgggtatt tactccgcgc gttattccgg tgaagacgcg accgatcaaa
    17341 agaatctgca aaaactgttg gaaacactga aagacgtacc ggacgaccaa cgtcaggcgc
    17401 gtttccactg cgtgctggta tatctgcgtc acgcggaaga tccgactccg ctggtatgcc
    17461 acggcagctg gccgggcgtg attactcgcg aaccagcggg cactggtggc tttggttatg
    17521 atccaatctt cttcgtacct tccgaaggga aaaccgccgc cgaactgacc cgcgaagaaa
    17581 agagcgccat ttcccaccgt ggtcaggcgt tgaaactgct gctggacgct ttacgtaatg
    17641 gttaaattac cgccgctgag tctctacatt cacatcccgt ggtgcgtgca gaaatgcccg
    17701 tactgcgatt tcaactctca cgcgttgaaa ggagaagtgc cgcacgacga ttatgttcag
    17761 catctgcttt gcgatctgga caatgatgtg gcttacgctc agggccgtga agtaaagaca
    17821 atctttattg gcggtggtac gccgagcctg ctttccggcc cggcgatgca aacgctgctg
    17881 gacggcgtgc gtgcgcgttt gccgctggca gcggatgcag aaattactat ggaagcgaac
    17941 cctggaacgg tagaagccga tcgctttgtc gattatcagc gtgctggtgt gaaccgcatc
    18001 tctattggtg tgcagagttt tagcgaagaa aagctgaaac gacttgggcg tattcatggc
    18061 ccgcaagaag cgaaacgcgc ggcaaagctg gcgagcgggc tggggctgcg tagctttaac
    18121 ctcgatttga tgcatggact accggatcaa tcactggaag aggcgcttgg cgatctacgc
    18181 caggccattg aactgaatcc gccgcatctt tcctggtatc aactgaccat cgaacccaat
    18241 acgctgtttg gttcgcgacc accggtgctg ccggacgatg acgcgctgtg ggatattttc
    18301 gaacaggggc atcagttatt aaccgcagcg ggttatcagc aatatgaaac ttccgcttac
    18361 gccaaacccg gttatcagtg ccagcacaat ctcaactact ggcgatttgg tgactacatc
    18421 ggtattggct gcggcgcgca cggcaaagtc accttcccgg atgggcgcat tctgcgtacc
    18481 actaaaacgc gtcatcctcg tggttttatg caaggaaggt atctggaaag ccagcgtgat
    18541 gtcgaagccg cagataagcc gtttgagttc tttatgaatc gcttccgtct gctggaggcc
    18601 gcgccgcgcg tggagtttat tgcgtatacc gggctttgcg aagatgtgat tcgcccacag
    18661 ttagacgagg cgattgccca gggttatctc accgaatgtg cggattactg gcagataacc
    18721 gagcacggta aattattctt aaactcactt cttgaattat tcctcgctga gtaatcatta
    18781 atgcgcctgc gggcgcatta atcacttctt ttccccccgt taattaactt cg
//
LOCUS       DAZRUF010000064        18548 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZRUF010000064 DAZRUF010000000
VERSION     DAZRUF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.064"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            53..1273
                     /locus_tag="ACHXOL_001773"
     CDS             53..1273
                     /locus_tag="ACHXOL_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR6808773.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKNSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPV
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1246..1875
                     /locus_tag="ACHXOL_001774"
     CDS             1246..1875
                     /locus_tag="ACHXOL_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR6808774.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVICLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1876..3036)
                     /gene="ybdL"
                     /locus_tag="ACHXOL_001775"
     CDS             complement(1876..3036)
                     /gene="ybdL"
                     /locus_tag="ACHXOL_001775"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR6808775.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3145..4233
                     /gene="hcxA"
                     /locus_tag="ACHXOL_001776"
     CDS             3145..4233
                     /gene="hcxA"
                     /locus_tag="ACHXOL_001776"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR6808776.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4243..4440)
                     /locus_tag="ACHXOL_001777"
     CDS             complement(4243..4440)
                     /locus_tag="ACHXOL_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR6808777.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4622..6727)
                     /gene="cstA"
                     /locus_tag="ACHXOL_001778"
     CDS             complement(4622..6727)
                     /gene="cstA"
                     /locus_tag="ACHXOL_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR6808778.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6908..7321)
                     /gene="entH"
                     /locus_tag="ACHXOL_001779"
     CDS             complement(6908..7321)
                     /gene="entH"
                     /locus_tag="ACHXOL_001779"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR6808779.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7324..8070)
                     /gene="entA"
                     /locus_tag="ACHXOL_001780"
     CDS             complement(7324..8070)
                     /gene="entA"
                     /locus_tag="ACHXOL_001780"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR6808780.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8070..8927)
                     /gene="entB"
                     /locus_tag="ACHXOL_001781"
     CDS             complement(8070..8927)
                     /gene="entB"
                     /locus_tag="ACHXOL_001781"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR6808781.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8941..10551)
                     /gene="entE"
                     /locus_tag="ACHXOL_001782"
     CDS             complement(8941..10551)
                     /gene="entE"
                     /locus_tag="ACHXOL_001782"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR6808782.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10561..11736)
                     /gene="entC"
                     /locus_tag="ACHXOL_001783"
     CDS             complement(10561..11736)
                     /gene="entC"
                     /locus_tag="ACHXOL_001783"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR6808783.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12111..13076
                     /gene="fepB"
                     /locus_tag="ACHXOL_001784"
     CDS             12111..13076
                     /gene="fepB"
                     /locus_tag="ACHXOL_001784"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR6808784.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(13157..14407)
                     /gene="entS"
                     /locus_tag="ACHXOL_001785"
     CDS             complement(13157..14407)
                     /gene="entS"
                     /locus_tag="ACHXOL_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR6808785.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14518..15522
                     /gene="fepD"
                     /locus_tag="ACHXOL_001786"
     CDS             14518..15522
                     /gene="fepD"
                     /locus_tag="ACHXOL_001786"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR6808786.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15519..16511
                     /gene="fepG"
                     /locus_tag="ACHXOL_001787"
     CDS             15519..16511
                     /gene="fepG"
                     /locus_tag="ACHXOL_001787"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR6808787.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16508..17323
                     /gene="fepC"
                     /locus_tag="ACHXOL_001788"
     CDS             16508..17323
                     /gene="fepC"
                     /locus_tag="ACHXOL_001788"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR6808788.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17320..18453)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHXOL_001789"
     CDS             complement(17320..18453)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHXOL_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR6808789.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4384 a         5239 c         4794 g         4131 t
ORIGIN      
        1 ttaacagtct tatctcactg ttcaccctgt aacgttcaga gagcgtcttc agatgtctat
       61 ttataaaatt cctcttccgc tcaatattct ggaagctgca cgagagcgta ttacctggac
      121 actcaacacc ctaccccgcg tttgtgtctc attttctggc ggcaaaaact ctggtttaat
      181 gctgcatctg acagccgaac ttgcccgaca aatgggcaaa aaaatctgcg ttttgtttat
      241 cgactgggag gcgcaattct cttgcaccat taactatgtt cagtccctgc gcgagttgta
      301 caccgatgtc atcgaagagt tttactgggt tgcgctcccg cttacgacgc aaaattccct
      361 ttcacaatac caacccgaat ggcagtgctg ggaacctgat gtcgaatggg tgcgtcagcc
      421 cccgcaagat gcgataaccg atcctgactt tttctgcttt taccagccag gcatgacctt
      481 cgaacaattt gtacgtgagt ttgccgaatg gttttcacaa aaacgtccgg tggcgatgat
      541 gatcggcatc cgtgcggatg agtcctacaa ccgttttgtc gccatcgcca gtttaaataa
      601 acaacgtttt gccgacgata aaccatggac tacggccgca ccaggcggtc atagctggta
      661 catttacccc atttacgact ggaaagtggc tgatatctgg acctggtatg caaatcatca
      721 aaatctctgc aacccactgt ataacctaat gtatcaggca ggcgttcctc tgcgtcatat
      781 gcgaatttgc gaaccttttg gcccggagca acgacaagga ttatggctct atcacgttat
      841 cgaaccggat cgctgggctg ctatgtgcgc acgagtcagc ggggtaaaaa gtggcggcat
      901 ttacgccgga catgataatc atttctatgg gcatcggaaa atcctcaagc cagaacattt
      961 agactggcaa gaatatgcat tattgctgct caatagcatg ccggaaaaaa cagctgagca
     1021 ttaccgcaat aaaatcgcca tttatttgca ctggtatcag aaaaaaggca tcgaggttcc
     1081 acaaacccag caaggggaca ttggcgcgaa agatatcccc tcctggcggc ggatatgcaa
     1141 agttttactc aataacgatt actggtgtcg ggcattatca tttagtccta cgaaagcgaa
     1201 gaactatcag cgttataacg aacggataaa aggaaaacgt caggaatggg ggatactatg
     1261 caacaacgat taacgcagga tttaacgcaa tttctcgcca gcttgccgga agatgatcgc
     1321 atcaaagcga ttaatgaaat ccgcatggcg atccatcagg ttagcccctt tcgtgaagag
     1381 ccagtggatt gcgttctgtg ggttaaaaac agccaactta tgcctaatga ttacaaccca
     1441 aacaacgtgg caccacccga aaaaaaactg ctgcaaaaat ccattgaaat agatggtttt
     1501 acccaaccca ttgtggtcac acatacggat aaaaatgcca tggaaatcgt ggatggtttt
     1561 caccgccatg aaattggtaa aggctcatca tcattaaaat tacgcttaaa aggttatttg
     1621 ccggtgatct gtctggaagg tacgcgtaat caacgcatcg ccgcgactat tcgtcataat
     1681 cgcgctcgtg gacgccatca aatcaccgcc atgtcggaaa tcgtccgtga acttagccag
     1741 ttgggatggg acgataataa aattggcaaa gagctgggaa tggacagcga cgaagtgctg
     1801 cgcctgaagc aaattaacgg cctgcaagag ctgtttgccg accgtcaata ttcccgcgcc
     1861 tggacggtta aataactaaa gctggcgcag gcgttcagct gctgccagca acgtcgattc
     1921 cttcttggca aaacagagac gaatcagttt atgtgggaag ggatcggcgc aaaacaccga
     1981 cagcggaatc gccgctacgc cgtgctcctg cgtcagccac tggcaaaact caacatcatc
     2041 cagggtagaa accgcgctgt aatccaccag caaaaagtat gtaccttcac acggtaaaat
     2101 ctccagccgg ctttcattta aggcatttac cagaatatcg cgcttctggc gataaaagtc
     2161 cggtaacgca agataatgct caggttctgc acgtagcata tcagcaagcg ccagctgtgc
     2221 cggggtattc accgaaaagg tcagatactg atgtaccttg cgaatttcgg cgctgatggg
     2281 cgctggcgca acacaataac ccactttcca gccggtcata tgataggtct tgccaaatga
     2341 agaaaccgcc actgcccgct cacgcagctg cgaatgcgcc agcacactgg catggccctg
     2401 ttgtgaaaag ttgatgtgct cgtagacttc atcgctaatg acaaaaatct cgtgcccggc
     2461 gatcgcctgc cacaaagcgg cgaaatcagc ctgctgccag acagttgcac tggagttatg
     2521 cggagtgttg aggatcacca gtctggtgcg ctcgcttaat aatgcggcaa attcctgcca
     2581 gtcaacgcga aaatgcggtg gttgcagtgc catacgcttc actattcccc cagaaagcgc
     2641 gatggcgggg gcgtaactgt catagctggg atcaaaacaa atcacttcat cgccattgcg
     2701 caccagtgcg gtaatcgccg cgtataacgc ttccgtcgcc cctgccgtta cggtgatatc
     2761 gctatcggca tctggttgat agccatacaa acgttccgtt ttctgagcaa tcgcctcgcg
     2821 caaggcctgc acgccggtca tgggcgcgta ttggtttgcc ccctgggcaa cgtggtacgc
     2881 cagccgctcc tgtaaatagc gcggaccatc aaaatcagga aagccttgcg acaggttaat
     2941 cgcctggtgt tgctgcgcca gcgcgctcat ctgggtgaaa atagtggtgc caagttgtgg
     3001 aagtttgctt tgtggaatca gagggttatt tgtcattttt attgtacctg acgaagttgc
     3061 gtgttgaagg cactataaca cgatgctagt atttggcaat caagacgttt agatgtctaa
     3121 ataaaacaat aaggacaaca caacatgcct cacaatccta tccgcgtggt cgtcggcccg
     3181 gctaactact tttcacatcc aggaagtttc aatcacctga acgatttttt cactgatgaa
     3241 caactttctc gcgcggtgtg gatctacggc aaacgcgcca ttgctgcggc gcaaaccaaa
     3301 cttccgccag cgtttggact tccaggggca aagcatattt tgtttcgtgg tcattgcagc
     3361 gaaagcgatg tacagcaact ggcggctgag tccggtgacg atcgcagcgt ggtgattggc
     3421 gtcggcggcg gcgcactgct cgacaccgcg aaagccctcg cccgccgtct cggtctgcca
     3481 tttgttgccg ttccgacgat cgccgctacc tgcgccgcct ggacaccgct ctccgtctgg
     3541 tataacgatg ccggacaggc gctgcattat gagattttcg acgacgccaa ttttatggtg
     3601 ctggtggaac cagagattat cctcaacgcg ccgcaacaat atctgctggc ggggatcggt
     3661 gacacgctgg cgaaatggta tgaagcggtg gtgctggctc cgcaaccaga aacgttgccg
     3721 ctaaccgtgc gactggggat caataatgcg caggccattc gcgacgtatt gttaaacagt
     3781 agcgaacagg cgctgagcga tcagcaaaat caacagttaa cgcaatcatt ttgcgatgtg
     3841 gtggatgcta ttattgctgg tggtgggatg gtcggtggtc tgggcgatcg ttttacgcgt
     3901 gtggcggcgg ctcatgccgt gcataacggt ctgactgtcc tgccgcaaac cgagaaattt
     3961 ctccacggca ccaaagtcgc ttatggcatt ctggtacaaa gcgccttgct aggtcaggat
     4021 gatgtgctgg cgcaattaac cggggcgtat cagcgttttc atctgccgac cacgctggcg
     4081 gagctggaaa tggatatcaa taatcatgcg gagatcgaca aagtgattac ccacaccctg
     4141 cgtccggtgg aatacattca ttacctgcca gtcacgctga caccagatac gttgcgtgca
     4201 gcgttcgaaa aagtggaatc gtttaaagcc tgacgtgcaa atttagcagc agcgcgcgcc
     4261 gccttttcca ccgtagcgcg cgtcctgccg ctcacggaaa aactcttcgt aggtcatcgg
     4321 cgtttgatcg ggatggttaa cccgcatatg ttcgacatag ttgtcgtaat caggcatacc
     4381 aatcatcagc ttcgccgcct gacctaaata ttttccggct tttgccagtg aatcaaacat
     4441 cttggcctcc gctaaccatg ttgctgaaag agtgccggat gcggcgtgaa cgccttatcc
     4501 ggcctacaaa tgctatcaac gcgctcggtc agtcccctcg ccccatcggg gagagggtta
     4561 gggtgaggag gaaccctcgg cactatgcca acattcgcca acatcccccc tcactctgac
     4621 tttagtgtgc gccttttgcc tgcgccacga tctcctcgac attttccggc attggctcat
     4681 acggcgtttc tttcgccgtt ggcttcggat ctttcaatgc cgccagtgcc gacttaatcg
     4741 agaacagcgc cagaaccacc acgaccacca taaagaagat ggttaacccg gcatccagac
     4801 ggttgttgaa caccagttgc gccagctgtg actcagtata ctgcgacgga atattgccgc
     4861 tgtcgatcat tgcctggaac ttattagcaa tggccaggaa gccgactttc gcatccgggc
     4921 taaacgcttt ctgccagcct gcggtcaggg tacaaatcag cagccaggcc gttggtacca
     4981 gcgccaccca ggcgtaacgt tgacgtttca tcttgaacaa caccacggca cagagcatca
     5041 gcgccatccc tgccagcatc tggttggcaa taccaaacag cggccacaga gtgttaatgc
     5101 cgcccagcgg atcgaccacg ccctgatgga ggaagtagcc ccacgccagt acgcacagcg
     5161 ctgttgccag caggttagca ggcagtgaat cggtccgctt caggccagga gacaccacgc
     5221 ccagcagatc ctgcaacata aagcgcgcag cacgcgtacc cgcatctacc gccgtcagaa
     5281 taaacagagc ttcaaacaga atggcgaagt gataccagaa cgccacatcc atcataccac
     5341 ccagcgcacc gtgcaggatg taagccatcc ccaccgccag cgtcggcgca ccgcccgcac
     5401 gggaaatgat cgactgttca cccacttcgc tggcaatctg gttgagcgta tccggagtaa
     5461 tcgcaaagcc ccagctactc accacctgcg cggcagaagc gaccacatcc gccgtccctg
     5521 ccggagccag caccgccatc gggctgttca tggcgaagta cacgcccgga tcgatgatac
     5581 aggcggaaac cagtgccata atcgccacga aggattccat taacattcca ccgtagccga
     5641 taaagcacgc ctgcccttcg ttcgccagca tcttcggcgt ggtgccggaa gagatcagcg
     5701 catggaagcc tgacaccgcg ccacaggcga tggtgataaa caggaacggg aacagattac
     5761 cggtccatac cgggccagtg ccatcgacaa atttggtcag cgcaggcatg gtcagcgtcg
     5821 gacgcataat caaaatgcct accgccagac caacgatagt cccgattttc aggaaggtag
     5881 agaggtagtc acgcggggcc agcagtaacc acaccggcag caccgccgcc acaaaaccgt
     5941 agcccaccag catccaggtc agctgcacgc cggtaaagtc aaagtacggt gcccaggtcg
     6001 ggctttctgc cacccagccg ccagagataa tggcaaaaat aaggaacacc aggccaatca
     6061 ctgacacttc gccaatacgc cccggacgca gatagcgcag gtagattccc ataaaaatcg
     6121 ccagcggaat ggtgaacgca acggtatacg ttccccacgg gctatgagtc agggctttca
     6181 ccacgatcat cgccagtact gcaaggataa tgaccatgat cataaagcag gctaccagcg
     6241 caatcacccc ggcagttggc cccatctctt ctttgaccag ctcacccagc gagcgaccgt
     6301 cacggcgcgt agaaacaaac agcaccatga aatcctgcac cgcaccggcg agaaccaccc
     6361 cggcgagcag ccagatcatc cccggcaggt agcccatttg cgctgccagc accggcccca
     6421 ccagcggccc tgctccggca atggccgcaa aatggtgacc gaacagcact ttcttgtccg
     6481 tcggcacata gtccagcccg tcgttatggc gcaccgctgg cgtcatacgc gtcgggtcaa
     6541 ccgccagcac atttttggcg atatacagcc cataaaaacg gtaagcgatc agatagatac
     6601 agaccgacgc caccacaatc cacagcgcgt tgatctgttc cccacgattt aaagcaatgt
     6661 atcccagagc aaatgctccc attacagaga gcactgtcca gacgaggtat ttccctgatt
     6721 tgttcatagt tgttatccgt gtgcgtgtcc atagagatgt tacattttgt ttctataaca
     6781 tctttgtacg atttaacaac acggtaacca tgtaaatcgg agttgaaacc aatatttaac
     6841 ttaacaatgt taactcgatc actccgttaa ccgagttttc ctgcaatctc atttaattct
     6901 gtcgggctca tcccaaaact gccgtaccca gccgacaagt gcagcaacgc cgcccctgtt
     6961 catcgaaaac gatgatttcc cagctctgat tttgccgccc aagatgcagc ggctggcaga
     7021 cgccgcgtac cttgccttca gacaccgggc gatgatgggt ggcgttaagt tccgtaccca
     7081 ccacacactg cccgtcacgg gtcatcataa atccggccat cgatcccagc gtttcagcca
     7141 gcgccgccga cgcgccgcca tgcagcaggc caaacggctg atgagtacgg gtatcaaccg
     7201 gcatttcggc ttccagcaca tcatctccca gacgggtata cacaattccc agatgcgcca
     7261 ccattgtgtt atcgctggtg gcgttcagtt cgtcgagcgt taaatggcgt ttccagatca
     7321 tgcttatgcc cccagcgttg agccgccatc gaccacaata tcctgtaggg tgatatggct
     7381 ggcgaggtca gaggcgagga acaaaatcgt gttggcgatc tcttgtggac gggcgatttt
     7441 ccccagcgga atgccgagtt taaactgctc gccaaagccg cgaatacgct gttcttcggc
     7501 gtcatcgctc acccacagcg tgcgttgcat atcggtgtcg gtggagccag gcgaaaccac
     7561 attacagcgc acgccgctaa ccgccagttc cagcccgacg ctcaacgcca ggcttttcag
     7621 cgccgctttc gatgcgccat aagcactcat accaatacgc ggcgtgtgtg cggcgtcgga
     7681 cgccacagtg acaatcgccc cgccccgctg acggcgaaac tggttcatgg tttgctgaaa
     7741 caggttaaac gcaccgccga cgttaaccgc aaaagtctgc tgccagtcct ctttactgag
     7801 ctgatcggtc gcgcccatgc gcaaaattcc cgccgcattg accagcacgt ccagtcgctc
     7861 cgtttcagct aacagtcgct gacatacctg cgcgacctgc gcagcatcgg caacatccat
     7921 cacttcggtc gcaaagggat attgctcctg agcgaacgct tgatcaaaac cggtaacttt
     7981 cgctcccgct tcaacaaacg ccagcgccgt ggcgtagccg atacctttac ctgcgccggt
     8041 tacccagaca tttttaccgc tgaaatccat tatttcacct cgcgggagag tagcttccac
     8101 caggcgtcga tggtcgggtt tttcgccagc atgacaaagt cgatatcgcc atgcactttg
     8161 cgccagcgcg ccgccagcgc catcatgcgc accgaatcca gaccgtagtc gatcaggttg
     8221 tcgtcatcga acggttcatc ggactcgtcc agcaacggca ggataacctc acgcagcgcc
     8281 gctttcctgg cggggacagg tgctggcagt aatccttcag tcatcaccac ccggccagac
     8341 cgcccggcca catatttcag cgacatcaaa tgctcgtcac ggctgaaatc ggccagcgca
     8401 tccgccacca taaacggttt aatatcgcgc ataaatgcgt cggttgcggt ggtcatacag
     8461 ccaatgtggg catatacccc ggtaataatc agctggttac gtccactctc tttgagcatt
     8521 tgctccagcg gagaacgatg aaacgcgctg tagcgccact tcaccagcac cgtgtcgtcg
     8581 gcatctggcg tcaggcgatc caccaccttt tgctgttccg gcgaacgggt caggcccggc
     8641 ccccacatat cattcaacag cgcccgatct tcatcgctct gctctttcgg ctgggcggtg
     8701 taataaaccg ggatattgtg ctgtttgcag tagtcgcgca gcgcagcaat attcgcgatc
     8761 acctgctcca tcatcgggca gttctcgccc cagaagctga caaaatagtc ctgcatatca
     8821 tggattaaca acgcggcacg ttgcggttca aaggcccagt caactttatt ctgcggaata
     8881 tcgtgagact ccggcagtgc gtaagcctgt aattttggaa tagccatcgt gttctctcct
     8941 tcaggctgat gcgcgtgacg ccagccactg acgtaattgt tttttatcga ctttcccgac
     9001 cgccgtcagc ggaagtgaat ccacacactc cacgcgatcc ggtaatttaa attcggcaat
     9061 accctgttca cgcaggaaac gacgcacctg caccgcgcgc agcggctctt ttaccaccag
     9121 ataagcacag cttttttcgc ccatcagctc atcttccatg ctcaccagtg cggcatagat
     9181 caccgccgga tggcgcagca gcaggttttc gatctcttcg gcagcgatct tctcgccgcc
     9241 acggttgatc tgatctttct cgcgcccctg cacggtgatg taaccctctg gatcaataga
     9301 gatcagatcg ccggaacagt aaaaaccgtt ggcatcaaag gcactggcat tgtgctgcgg
     9361 acttttgtaa taaccgcgga aggtgtacgg cccgcgcgtc atcaggcgtc caacttcccc
     9421 ttgcggcagt ggatttcctt cagcatcagc aacccatact tcatcgtccg gacacattgg
     9481 gtaaccctgg gtatggataa ttttctccgc gctatcatca agtcgggtgt agttcaccag
     9541 cccttccgcc atgccaaaca cctgctgcaa ctggcagcca atctcagcgg aaatacgcgc
     9601 cgcaagggtg gcagaaagac gtgcgccgcc gacctgtaac agtttcagcg aggcaagctg
     9661 cgcccggctt tcgccttcag ccagcgcctg caaccacagg ctgactgccg gcggcaccag
     9721 cgcggtaacg ttaacctgat gttttgcaat caacgggaag caaagcgtgg cgctgggatc
     9781 ggccgccaga acaaccgttc ctccggcaag aaagacgccc agcgatcctg gcgaactcat
     9841 agcgtagtta tgagccgccg ggatcgcgca caggtagcgt gtctgttgtg tgaactgaca
     9901 aatctcgacg ctacgacgca cgctgtagta gtagtcgtta tgagtgcgcg ggatcagctt
     9961 cggtgtaccg gtggtgccgc cggaaagctg gaaataggcc acttcatcag caggtgatgg
    10021 cgtagcagta aaaccgtctg ccggatggtt aatcgcatcc tgcaagttat gctcaccgct
    10081 gtcgttgagc agttgcacca cgcgaatgga ggaatgttct gtgacgaaag tattgaggaa
    10141 atcatccccg ctaaacagcg catgttggcg atcggcaatc agtaacgccg gttcaatctg
    10201 gctggcatag gcgttcagtt cactacgctg atggctgaac aacgccagca ccggcgcaac
    10261 gcccagtttc agcagtgcga aaaaggtaat gtaaagttca gcgacgttac ccagttgtac
    10321 cagcgcggtt tcaccgggtt taatgccctg acggcgtaaa ctacacgcga ggttatccgc
    10381 cgcctgattc agctcccggt aactcaactg tcgctcgccg tcgataaccg cgatgctgtc
    10441 actcgcagcg tggcgagtca gaatgtcggt cagcggcaaa tcctgccagt agcctttttc
    10501 ccgatagcga cgggcaaact cttccggcca gcgggtgaat ggaatgctca tcctcgctcc
    10561 ttaatgcaat ccaaaaacgt tcaacatggt agaaagtttg acgcctgttt cgcgccactc
    10621 acccaacggt gacgacgcag gcacaatccc cgctccggca aacagacgca cctgattttc
    10681 ccgcagcttc gcgcagcgga tggtcaccac ccattcgccg ttaccttcgc tgtcacacca
    10741 acccacaatg ccgccaaaca gttcgcggtc aaacggctcc agttcagcaa taacctgggt
    10801 cgcggcctga tgcgggaaac cgctcagcgc gggggtcgga tgcagcagac aggccagagt
    10861 cagtgcgttt tcttgcgaat tcgctttacc ttcaaaggga gttgcgagat gccacagcgt
    10921 cggcgtggta atcaattgtg gagaggaggg aacgtgtaac tcactactgc gttcgcgcag
    10981 tacctctttc atcgcctgag tcaccagttc atgttcatgg cgatcttttt ctgacgccag
    11041 cagacgatta cccgcttcgc gatcgagcac ttcatccggc tgacgacgcg cggaaccggc
    11101 taacggaatg gagctaaaac gctcgccgtc tttacgtagc agcagttccg ggctggcccc
    11161 cagcaggacg ccaccatcag ccagcgggac atggaagttg taactaaccg ggttttgcgc
    11221 aatcaaccgt tccagcaata cgccactatc aatggcggcg tcagtggtga tatcaatcaa
    11281 tcgtgacaac accactttgt cgacctgcgg cgtggcggta agtgcggcgg cgcgggcaac
    11341 catctgttca aacgtggttt gttcaggaat tgcctggcgt tccaccacgt tcagcgactg
    11401 gctgcgggta aaacagtggg ctgaggtctg tttttcctga cgggagaacg actgccagga
    11461 ttcgggaata tacagcgacg aaggctgacg tggatcgaag ggaatcgccc cgaccatcac
    11521 cggatttttg atgccctgcg ctttggcatc ggcaaacagc gcggcgagtt tttgctggaa
    11581 gggactgtcg ggcgaatccc cgttcacagc cggttcatcg aagcgggcga aacatcctga
    11641 cgtcgtaaaa ctgcggtacg gcgacataaa gaaaaagcga ttgggcgcaa gtgttgccat
    11701 ggtctgctgt acttcctcag ccagtgacgt atccatatca tcctccacaa aatgataaag
    11761 gctttaataa tgattatcat ttatattttc ggtcgctaac ctaaaagcaa acgcgcacta
    11821 tgtcaactct tgaggtaacg caatctgcaa ctcggggttg catctggttg tggttacaat
    11881 gaaaatgaga agcattattg atgaattcgc ataagcgcaa tgtgatgtcc tgcgccgttt
    11941 tgccccctct cccttccagg gtgagggctg gggtgagggt taatgttcgc accagtgctg
    12001 gctgttcccc tcaccctaac cctctcccca aaggggcgag gggacggatt gtgcgctttg
    12061 tcgaatttgt cattacgccc ttaaccttat taataacagg aagctgactt gtgagactcg
    12121 ccccgctcta ccgcaacgcc cttctattaa caggactttt gctttcagga atagccgtag
    12181 ttcaggccgc cgactggccg cgtcagatta ctgacagccg gggcactcat accctggaaa
    12241 gccagccgca gcgtattgtt tccaccagcg tcaccctgac cggatcactg ctggcgattg
    12301 atgctccggt gatcgccagc ggcgcgacca cgccgaataa ccgcgtcgca gatgaccagg
    12361 gctttttacg ccagtggagc aaggtggcga aagaacgcaa actgcaacgg ctctatatcg
    12421 gcgaaccgag cgccgaagcc gttgccgcgc aaatgccgga tctgatttta attagcgcaa
    12481 ccggcgggga ttcggcgctg gcgctgtatg atcagctttc caccatcgcc ccgacattaa
    12541 tcatcaatta cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg
    12601 ggcatgagaa acaagcggca gagcggattg cacagtttga taagcaactg gcagcggcga
    12661 aagagcaaat caaattaccg ccgcagccgg tcactgccat tgtctatacc gccgctgcac
    12721 acagtgccaa tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct
    12781 ttacgctggc gaagttgccc gcagggttaa acgccagcca aagccagggc aaacgccatg
    12841 acatcattca gcttggtggg gaaaatctgg ctgctgggtt aaacggtgaa tcgctattcc
    12901 tgttcgccgg tgatcagaaa gacgccgatg cgatttatgc caatccactg ctcgcgcacc
    12961 tgcctgcagt acaaaacaag caggtttata cgctgggaac cgagacgttc cgcctggatt
    13021 actacagcgc catgcaagtg ctggacagac ttaattcact gttaaaaaaa gattgataaa
    13081 acatacaatt agagcactaa taatcccctc gcccctttgg ggagagggtt agggtgaggg
    13141 gaaaatgcac ctttagctaa ctgtcagacg ctgtcacctg cggcggcgtc tggcgaaaac
    13201 gtcgcaactc caccagcacc agcaataaca acacgccgat aatcaacaaa ccaaaaccgc
    13261 tcgcgcttgc ggaagcaacc ggtgtcatca tcgcgcccaa accacccaac agcgccgcgc
    13321 ctatggcatc gcccgtcacg ttctgcgccg tccacaaacc gttaatccgc cctaacatcg
    13381 cttccggggt ttgcgtttgc agcattgtat attgcagcaa cgaactaacc gcgctcagcc
    13441 agccgaacag cgccagacaa accacgccta aaatccacat cggcatcagg ccaaacagac
    13501 caatggcgag gaacgatccc agcgtggaga gcagcatcaa taaccctggt cgcgcactat
    13561 gtgccagctt cccgctggtt aacgcaccaa tagccgcacc gagcgggatc gccgcataga
    13621 gaaaaccaat ctgtgccgcc gacatctgcc agttgtcagc cagcgccgga tacagtaccc
    13681 gcaccgcgct cgccatcgtc aataaaccac ccagcaacgc aatccctcct accagcgggc
    13741 tggcgagcag aaaacgaaat cctgccagta atgatttcaa cggatgctcg cgcggctgcg
    13801 gtggcggtgg caacgccgga aggcttaaca acggtagcaa ggtaataaac gtgcccgccg
    13861 ccgccagccc gtagttccag gctacgccgc cggtcgccag caataaaccg ccaatcatgg
    13921 gcgaaatcac cgaccccaga cgcacggtca acatggtgat cgccccggcc tgcattaagt
    13981 tttcacgccc taccagtgca ggtgttgccg ccagtagcgc cgtaacacca agtgatgcga
    14041 aaaaaccatc ccataaacca agtaaataga ttgccagcaa tgacggctcc ggcagcagcg
    14101 cgttcaggca cagtccaatg aagccaatgc cacaggtgcc gcgcgccagc aaaatcactt
    14161 ttttgcgctc atagcgatcc gccagcacac cgccaaccat caggccaaca aacatcgcgc
    14221 cgccggtcag cgtcaccgaa agccccacct gccaggtaga atgcgtcatc atctggatct
    14281 gcaccggcac cgcgacgccg agcaaaccca gagacacaat tgagataaaa cgagcgagga
    14341 atactgcgcg aaacgccggg tgcgttttca acaggctgag gttaagcagc caggattgtt
    14401 tattcattac aatgccttgc catcaaatat tttaaatacc tattcttagg ctgcacatgc
    14461 taacatatcc aaataagatc gataacgata attaatttca ttatcatgga agttcgtatg
    14521 tctggttctg ttgccgtgac acgcgccatt gccgtgcccg gattgctgtt attactgatt
    14581 atcgcgacgg cattaagcct gctcattggg gcaaaatcac tccccgcgtc agtagtgctg
    14641 gaggccttct caggcacctg ccagagcgcc gactgcacca tcgtgctcga cgcgcgactg
    14701 ccgcgcaccc ttgccggttt acttgcaggc ggtgcgcttg ggcttgctgg ggcgttaatg
    14761 caaaccctca cccgaaatcc acttgccgac cccggcttgc ttggcgtgaa cgccggagcc
    14821 agctttgcca ttgtgctggg tgcggcgctg tttggttact catccgcgca ggaacaactg
    14881 gcgatggcct tcgccggggc gctggtggcc tcattgattg ttgcctttat cggcagtcag
    14941 ggcggcgggc agttaagtcc ggtgcgttta accctggcgg gcgtggcgct ggcggcggtg
    15001 ctggaagggc tgaccagcgg catcgccctg cttaatcctg acgtctacga tcaactgcgt
    15061 ttctggcaag ccggttcgct ggatattcgc aatctacata ccttaaaagt ggtgctgatc
    15121 ccggttctga tcgccggagc aactgcgcta ttactgagtc gcgcgctgaa cagtttgagc
    15181 ctcggcagtg acaccgcgac ggcgctgggc agtcgcgtgg cgcgcacaca gttgattggt
    15241 ctgctggcga ttaccgtgct ttgtggtagt gcgacggcgg tagttggccc gattgccttt
    15301 atcggcctga tgatgccgca catggcgcgc tggctggtag gtgccgatca tcgctggtcg
    15361 ctgcccgtca cgctacttgc tacccctgcc ctgctgctgt ttgccgatat catcgggcgg
    15421 gtgattgtgc ccggcgaact gcgcgtttct gtggtcagtg cgtttattgg tgcaccggtg
    15481 ctgatcttcc tcgtgcgacg taaaacgcga ggtggtgcat gatttacgtc tctcgccgat
    15541 tactcatcac ctgtttgctg ctgatttccg cctgtgtggt tgcaggtatc tggggattac
    15601 gcagcggtgc cgtcacgctg gaaatctcgc aggtattcgc cgcgttgatg ggcgatgcgc
    15661 cgcgcagtat gacgatggtg gtcaccgaat ggcgtttacc acgcgtgctg atggcgctgt
    15721 tgattggcgc ggcgctgggc gtcagcggcg cgatttttca gtcgctgatg cgtaacccgc
    15781 tcggcagccc tgacgtaatg ggctttaaca ccggggcgtg gagcggcgtg ctggtggcga
    15841 tggtgctgtt tggtcaggac ctgacggcta tcgcgctggc agcaatggtg ggcggcattg
    15901 tcacttctct gttggtctgg ctgctcgcct ggcgtaacgg tatcgacacc tttcggttga
    15961 ttattatcgg catcggcgtt cgcgccatgc tggtggcctt taatacttgg ctgttgctga
    16021 aagcgtcttt agaaacggcg ctaacagcgg gtttgtggaa tgccggatcg ctcaatggcc
    16081 tgacgtgggc aaaaacttcg ccttccgcac ccatcatcat attgatgctc attgccgccg
    16141 ccttgctggt gcggcgaatg cgcttgctgg agatgggcga tgataccgcc tgcgcgctgg
    16201 gcgtcagcgt cgagcgttcg cgtctgttaa tgatgctggt tgcagtggtg cttaccgctg
    16261 cggcaacggc gcttgccggg ccgatttcct ttattgcttt agtcgcgccg cacattgccc
    16321 gacgcattag cggcaccgct cgctgggggc tgacccaggc ggcgctgtgc ggagcgctgt
    16381 tactgctggc ggccgatctc tgcgcccaac aactgtttat gccgtatcaa cttccggttg
    16441 gcgtcgttac cgtcagcctc ggcggtattt accttatcgt cttgttaatt caggagtctc
    16501 gcaaaaaatg accgaatcag tagcccgttt gcgcggcgaa cagttaaccc tgggatatgg
    16561 caaatatacc gttgcggaaa atctgactgt agaaattcct gatggtcact tcacggcaat
    16621 tatcggacca aatggctgtg gtaaatccac gttactgcgt accttaagcc gcctgatgac
    16681 gcctgctcac gggcatgtct ggctggatgg cgagcacatt caacattacg ccagtaaaga
    16741 agttgcacgc cggattggtc tgttggcgca aaatgctacc acgccgggcg atatcaccgt
    16801 gcaggagctg gtggcgcgtg gacgttatcc gcatcaaccg ctgtttaccc gctggcgcaa
    16861 agaggatgaa gaagcggtaa cgaaagcgat gcaggccacg agaataactc atctggcaga
    16921 tcaaagcgtg gatacccttt ctggcggaca acgccagcga gcgtggatcg cgatggtgct
    16981 agcccaggaa acggcaatta tgctgctcga cgaacccacg acctggctgg atatcagtca
    17041 tcagattgat ctactggaac tgttaagcga actgaaccgc gagaaaggct ataccctggc
    17101 ggcggtgctg cacgatctta atcaggcctg tcgttacgcc agccatttga ttgcattgcg
    17161 ggaagggaaa attgttgctc agggagcgcc gaaggagatt gtcactgctg aactgattga
    17221 gcgcatttat ggtctgcgat gcatgatcat tgacgatccg gtagccggaa cgccgcttgt
    17281 ggtgccgctc ggacgaacgg caccttcaac cgcaaatagt taaactaagt ggtctgccat
    17341 catggcatcc tgttttctgg atgccatcgc atagcgcaat aacacgctac cacaagccac
    17401 catcccgccg atcagcgcgg aaaggatcac aataatcgcc ttacccggac cgtctttttt
    17461 cactggcaat gacgggctta actgatattt aaacggcgta aaattcacat cgttgacatt
    17521 tgcttttgtt aattgctcga caagatactg ccgattacgt aattcaccgt tcagttccgc
    17581 aacgtcagta accgcttttt caatttccag tttgcgttca ataccgtctg cgccgagaga
    17641 aatagagaaa tcggggtcat ctttaacggc ctgaccatta ctgtatacgg gctttttaat
    17701 tcctgccgcg ttggcaatgt cgagtgaata attgaggcgc tgaatgtttg catcaagttg
    17761 atttttcgtt ttaatgcgat cctgagccag tttttctttt tcaaactggg ttttgatctc
    17821 cagtttatta cggacgtttt ctatcgactc tttcaccacc aacgcagaga tataatcgat
    17881 atacccgctc aaaacggtct gcgcctcttt actggttggc gcggtaaaac ttagcgtcca
    17941 ggaggtatac agtgacggtt catctttttt cttactggca ttgtcatcaa ccgctttcat
    18001 tttttcgctc aatgcaacaa tagcgcgatg caaatccagt tcatcgattt tcgcctcttt
    18061 taattggtcc atcacataag gtgatgaacg caggtactct tccagcaagc taacagactg
    18121 aaacttcttg ataaacaggt taaatgcacc gccacggtcg atattaacat cgagatcaag
    18181 aacgcggagt ttggtaaagg ttttctccag gtcctgccac tgaatggctt cggcgggagt
    18241 aataacggct gagctggtcc atttttgcgg caggatgaaa gagatcagta aacctgcgca
    18301 ggcaaatgca aaaacgaccg ccatgaccgt ttttttggcc cgccataaaa cttcgattag
    18361 attaagtaaa tcaatttcat tattactggg cgacgccaga ggatactcag aaaaatgagc
    18421 gtcacttccc tgtttaatat tcaatgatga catggggtat aacctgaaaa tgagtccaat
    18481 aggggaaaat ctccacggcg ctcactttac cagttggtga gaattatccg aagctgaagt
    18541 ttgtaaat
//
LOCUS       DAZRUF010000065        18415 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZRUF010000065 DAZRUF010000000
VERSION     DAZRUF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18415
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.065"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..265)
                     /locus_tag="ACHXOL_001790"
     CDS             complement(<1..265)
                     /locus_tag="ACHXOL_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR6808790.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDT"
     gene            322..990
                     /locus_tag="ACHXOL_001791"
     CDS             322..990
                     /locus_tag="ACHXOL_001791"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR6808791.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTRVQGTVVMFSA
                     SGKK"
     gene            complement(935..1072)
                     /locus_tag="ACHXOL_001792"
     CDS             complement(935..1072)
                     /locus_tag="ACHXOL_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR6808792.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1045..1143)
                     /locus_tag="ACHXOL_001793"
                     /pseudo
     CDS             complement(1045..1143)
                     /locus_tag="ACHXOL_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(1132..1371)
                     /locus_tag="ACHXOL_001794"
                     /pseudo
     CDS             complement(1132..1371)
                     /locus_tag="ACHXOL_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1497..2339
                     /locus_tag="ACHXOL_001795"
     CDS             1497..2339
                     /locus_tag="ACHXOL_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808793.1"
                     /translation="MTGELKIGTVNALRIFNDTFGLIFRRSEDFLHFIPTAEGQGENG
                     NIGPLRPFAINLRTGAISVSHGAKIKGGLAIGATDNALGENSIVLGDNDTGFRQDGDG
                     IISFYSNGSRIGHIDELGLHLYKDIESNGSNFRLKSNYRHHITFANEDGRIRMFLWKD
                     NGGDGVHINNGSDGGGDFIFKTDGGFALGSGAQVASSGDIYGSVWENNWLSTWLHNHV
                     VRDIRLGSIEYKNVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYN
                     VGSV"
     gene            2341..2868
                     /locus_tag="ACHXOL_001796"
     CDS             2341..2868
                     /locus_tag="ACHXOL_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR6808794.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2897..3430)
                     /locus_tag="ACHXOL_001797"
     CDS             complement(2897..3430)
                     /locus_tag="ACHXOL_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR6808795.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(3433..3579)
                     /locus_tag="ACHXOL_001798"
     CDS             complement(3433..3579)
                     /locus_tag="ACHXOL_001798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808796.1"
                     /translation="MKKGVMYEKSGHVITGLGIIGEVDGDDPAVFRPIQKLINGTWYN
                     VSQV"
     gene            complement(3583..6210)
                     /locus_tag="ACHXOL_001799"
     CDS             complement(3583..6210)
                     /locus_tag="ACHXOL_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR6808797.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTMVVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFEQMVEEVSRNASAVAQNTAAAKKSASEAATHATDAAASARAASTSAGQAASSAQ
                     SASSSAGTASTKAREAAKSAAAESSKSAAATSASAAKTSETNAAASQKSAATSASTAT
                     TKASEAATSARGAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSS
                     ETAAGQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSASTATTKAG
                     KATEQATAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASSASASKDEA
                     TRQASAAKGSATTATTKASEAAGSATAASQSKVAAESAATRAETAAKRAEDIASAVAL
                     EDASTTKKGIVQLSSATNSTSESLAATPKAVKAVMGETNKKAPLNSPALTGTPTTPTA
                     RQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQ
                     PKDATLTALAGLTTAAGKFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLREL
                     GTSGEKIPLLSTANTWTNRQTFSGGLSGELSGNASTAAKLKTARKISNVAFDGSSDIT
                     LKASHVGAFALGKTGSTVANDKAVGWNWSSGAYNATISGASTLIIHFYMGEGSCPAAQ
                     FRINYKNGGIFYRSARDGYGFEADWSEFYTTRKPSAGDVGALPLSGGQLNGALGIGTS
                     SALGGNSIVLGDNDTGFKQKGDGNLDVYANNVHVMRFVSGSIQSNKTINITGRVNPSD
                     YGNFDSRYVRDVRLGTRVV"
     gene            complement(6362..6961)
                     /locus_tag="ACHXOL_001800"
     CDS             complement(6362..6961)
                     /locus_tag="ACHXOL_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR6808798.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMVGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRTDAFIVGIGYRI"
     gene            complement(7029..10463)
                     /locus_tag="ACHXOL_001801"
                     /pseudo
     CDS             complement(7029..10463)
                     /locus_tag="ACHXOL_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(10807..11487)
                     /locus_tag="ACHXOL_001802"
     CDS             complement(10807..11487)
                     /locus_tag="ACHXOL_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR6808799.1"
                     /translation="MATTNAFSLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            complement(11385..12128)
                     /locus_tag="ACHXOL_001803"
     CDS             complement(11385..12128)
                     /locus_tag="ACHXOL_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR6808800.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAALACM"
     gene            complement(12139..12837)
                     /locus_tag="ACHXOL_001804"
     CDS             complement(12139..12837)
                     /locus_tag="ACHXOL_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR6808801.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(12837..13166)
                     /locus_tag="ACHXOL_001805"
     CDS             complement(12837..13166)
                     /locus_tag="ACHXOL_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808802.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(13163..15736)
                     /locus_tag="ACHXOL_001806"
     CDS             complement(13163..15736)
                     /locus_tag="ACHXOL_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR6808803.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLADAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKIEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWS
                     AEEQLRGSWMAGMKSGWGEWAESATDSFSQVKNAATQTFDGIAQNMAAMLIGSEQNWR
                     GFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATG
                     GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMR
                     RAEGINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSG
                     G"
     gene            complement(15717..16130)
                     /locus_tag="ACHXOL_001807"
     CDS             complement(15717..16130)
                     /locus_tag="ACHXOL_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR6808804.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(16157..16588)
                     /locus_tag="ACHXOL_001808"
     CDS             complement(16157..16588)
                     /locus_tag="ACHXOL_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR6808805.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(16602..17354)
                     /locus_tag="ACHXOL_001809"
     CDS             complement(16602..17354)
                     /locus_tag="ACHXOL_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808806.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(17362..17757)
                     /locus_tag="ACHXOL_001810"
     CDS             complement(17362..17757)
                     /locus_tag="ACHXOL_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR6808807.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(17754..18329)
                     /locus_tag="ACHXOL_001811"
     CDS             complement(17754..18329)
                     /locus_tag="ACHXOL_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808808.1"
                     /translation="MAIKGLDQAIDNLNRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            complement(18344..>18415)
                     /locus_tag="ACHXOL_001812"
     CDS             complement(18344..>18415)
                     /locus_tag="ACHXOL_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR6808809.1"
                     /translation="PDDGGSCYLWLNRGQPPAVNRRR"
BASE COUNT         4050 a         5365 c         4432 g         4568 t
ORIGIN      
        1 ttgtgtctgg ttcttccggc cactcaatat caggtgcagt tgatgtatca acacggttca
       61 gcaacacccg atacttcttc caggcttcca gcaacgagtt ttcttcctcc gttgcgatct
      121 ccagatctac agcatcctga agtggcgcaa tatgctcact gaattcctgg atgtagaact
      181 gtgtggtgac ggtcttccag ccattcggct cctgctgtat cgaagcatac caggctattt
      241 caatatcgct atgctgcggc agcatttaac cccttgtaat tcatcgccat aattgattta
      301 attcacaaat aaaactataa tatggtgaaa ttaatgaaaa aaaacacaga tgatggggct
      361 aaaatttaca caccacttac cctaaagctt tatgactggt gggttttggg agtatcaaat
      421 cggcttgcat ggggatgtcc tacaaaggaa caccttcttc cacactttct ggaacattta
      481 ggtaacaacc atctggatat tggtgttgga actgggtttt accttactca cgtacctgag
      541 agtagtctga tatctttaat ggatttgaac gaagctagcc tgaacgcggc atctacaagg
      601 gctggggaat caaaaattaa acataaaatt agccatgatg tttttgaacc ttatcccgcg
      661 gcgttacatg gtcaatttga ttccatttcc atgttttacc ttcttcactg cctgcctgga
      721 aatatatcta caaaaagctg tgtaatacgc aatgcggcgc aggccttaac tgacgatgga
      781 actctatacg gagccacaat tcttggcgat ggagttgtgc acaatagctt cggtcaaaaa
      841 ctgatgcgca tttacaatca gaaaggcatc ttttcaaaca caaaagattc cgaagaaggc
      901 ttaacacata tactctcaga gcatttcgag aatgttaaaa ccagggttca aggtactgta
      961 gtaatgtttt ccgcttcagg gaaaaaatag catccaaccg cagcacgttc ttgcttaaga
     1021 cgtgctgcgg cataatccca atgattactc cctgacaggg ttcgtaggcc actcaatatc
     1081 aggtgcagtt gatgtatcaa cacggttcag caacacccga tactttttct aggcttccac
     1141 caccagcacg acaagatgcc gcatacagtg acccagtcag tccagttttc agacaaccag
     1201 tgcgtcacct ttttgaaggc gctttaaagc acgttttaat ccaggtcggc ctgtccttgt
     1261 tccgcttaat ttatcttcaa atatttgttc atatcctgca caaacaagag cgtttcgttg
     1321 caggtctgta ttctggtcat ttgttgatac ccttacatag ccaatcagca cgctgaatct
     1381 cccgtccaaa agcacaaatc atgccatgca ggccagaaac cgccattatc taaaacctcg
     1441 gtttacagga aacggtaaac aaggctggta acgccgttca gcgttccggc gataaaatga
     1501 ccggagaact gaaaattggc acggtgaatg cgctgcgaat tttcaatgat accttcggtc
     1561 taattttccg tcgttcagaa gattttcttc atttcattcc gacggctgaa ggacaaggcg
     1621 aaaacggtaa tatcggccca ttaaggccat tcgctataaa tctgagaaca ggtgctatat
     1681 ctgtcagcca cggggccaaa attaaaggag gtctagctat tggagctact gataacgcac
     1741 tgggtgaaaa ctccattgtt ctgggagata acgacaccgg atttaggcaa gatggagatg
     1801 gtattattag cttctattca aatggttcgc gcatcggaca tattgatgag ttaggattac
     1861 atctttataa agatattgaa tctaatggca gcaattttag attaaaaagt aattaccgcc
     1921 accacattac attcgccaac gaagacggaa gaattcgtat gtttttgtgg aaagataacg
     1981 gtggtgatgg tgttcatatt aataacggtt cagatggtgg tggtgatttc atttttaaaa
     2041 cagatggggg atttgcgctg ggaagtggtg cgcaagttgc gtctagtggc gatatttatg
     2101 gttcagtgtg ggaaaacaac tggttaagca catggctgca taatcatgtc gttcgggata
     2161 ttcgtcttgg cagcattgaa tataaaaacg tatggcgcga ctacggcttt ggcgatgcgt
     2221 caggttatgt tttaacagcc gcaattaaca gcaatgcgga tgatattgtc gacactgttg
     2281 ccagaaggcc aattcagaaa ttgattgggg gaatatggta caacgtgggg agtgtttaag
     2341 atgatgcacc tgaaaaatat taaggccggg aatccgaaaa caccggaaca ataccagcta
     2401 actaaaaaag cgggcgtggt atggctcttt tgcgaagatg gcaaaaactg gtatgaggaa
     2461 caaaagaatt ttcagccaga caccataaaa attgcttacg atgagaacaa catcatcgtt
     2521 gctgtcagta aagacgtttc aactattaat cctgaagggc taagcatagt tgaagttcct
     2581 gatattacgg caaatcgtca cgcagacaat aacggaaaat ggatgtttct ggatggtaaa
     2641 gtagtaaaga gggaatatac agaacaggaa ctgcaacaac aggccgaatc acaaaaggcc
     2701 gcattgcttt ccgaagctga atcagtcatc cagccgctgg aacgcgctgt caggctgaat
     2761 atggcaacag acgaggaacg cacacgactg gaagcatggg aacgctacag tgttctggtc
     2821 agccgtgtgg atacggcaaa tcctgaatgg ccacaaaagc ctgaataaaa attaaggccc
     2881 gatagcgggc cttctctcat tctggttgtt cgggaaacgt tactggcagg ctggaggtgt
     2941 ctgtggattc aaccttctgc gcatagagca tccactcggt taatttttgt ttattctcat
     3001 cggaaatgat gcccagctgt agctgagagt cccacagctg ggttttatcc ctgacgagct
     3061 gcagcaggct ctgcttttca ttttccgctt gttgcctttg ctcttcctcg gtataagttc
     3121 gctttatcac cgcaccatct ttgaacatcc agttgcccga aatatcagcc cggcgatttg
     3181 ctgtaatatc aggcaactca acgacactta aaccttctgg attaattgct gatacatcct
     3241 tgtctatagc aataattatt ccctcaactg tgtaagccag ttttattgta tcctcctgaa
     3301 agttttttaa ttcctcatgc caattttttc catcttcgga ccataaccag ataacatcaa
     3361 aattctttgt tagctggtat tgttcaacgg tttttggatt acctgcgaca atgtttttta
     3421 aatgctgcat gattatacct gcgatacgtt ataccatgtt ccgttaatta acttttgtat
     3481 tggtctgaat actgccggat catcaccatc gacttcaccg ataataccaa gccccgtgat
     3541 tacatggcct gatttttcat acatcacgcc tttcttcatg gtctaaacaa ctcgtgtgcc
     3601 aagtctgaca tctctcacat agcgggaatc aaagttaccg taatccgagg gattaacacg
     3661 tcccgtaata tttatggttt tattactttg aatgcttccg gagacaaagc gcataacatg
     3721 gacgttatta gcataaacat ccagattacc atcgcctttt tgtttaaagc ccgtgtcatt
     3781 atcacccaat acaatcgaat taccgccaag agcactggat gttccgatac ccagtgcacc
     3841 attcagttga ccaccagata acggcagtgc accaacatct cctgctgaag gttttctggt
     3901 ggtgtaaaat tcggaccagt cggcttcaaa accataacca tcacgggctg aacgataaaa
     3961 aataccgcca tttttataat taatccgaaa ctgagctgca ggacaacttc cttctcccat
     4021 ataaaaatga ataattaacg ttgatgcacc actaatagtt gcgttatagg ctccgctact
     4081 ccagttccat ccaactgctt tatcattcgc aacggtgctt cctgttttcc ctaaggcaaa
     4141 cgcaccaaca tgacttgctt ttaatgtgat atcggaggaa ccatcaaaag ccacattgct
     4201 tattttcctg gcagttttta attttgcagc tgtagaagca ttgccggata gttcaccaga
     4261 aaggccaccg ctgaatgttt gtcgattagt ccaggtattc gctgtactga gtaacggtat
     4321 tttctccccg cttgtgccga gttctcgtaa accgaggtat tcgataacgg cagcaacggt
     4381 cgatttcgca agaatatccc gcccgacttt tgtcagggtt gccaggctgg cgacatcatt
     4441 ccccgtaaaa tacggaaact tgcctgccgc agtagtaagt ccggccagcg ccgtcagggt
     4501 ggcatctttc ggttgcttac ccgcaagcgc gttagtcatg gtggtcgcaa aattcgggtc
     4561 gttgcccagc gccgcagcca gctcgttcag cgtgttcagt gcatcaggtg acgaatctac
     4621 aagggcggca atcgcagcca taacgaaagc cgtgcttgcg atttgggtat tattcgttcc
     4681 ctgtcgcgca gttggtgttg ttggcgttcc ggtcagtgca ggactattta agggcgcttt
     4741 cttgttcgtt tcacccatta ccgccttaac cgcttttggc gttgccgcca gtgactcgga
     4801 agtgctgttg gtcgcactgc tgagctgtac tatccccttt ttcgtcgtgc tcgcatcctc
     4861 cagcgccacg gcggatgcaa tatcctctgc ccgttttgct gctgtctcgg cgcgcgttgc
     4921 cgcggattca gcagcaactt tgctctgaga tgcagccgtc gcactgcctg ccgcctctga
     4981 tgctttcgtt gttgctgtcg tggcactacc tttcgctgct gacgcttgtc tggtcgcctc
     5041 atcttttgaa gcagacgcag atgatgccga tgacgccgct gaactggctg acgatgcggc
     5101 tgctgcctta gaggaagcag cattgtctgc tgaagtcttt gcatttgttt cagaggtttt
     5161 tgctgcagaa gcagacctcg ctgctgcagt ggcttgctca gtggctttgc cagccttcgt
     5221 tgtggctgtt gaagcggatg atgcggcgct ttctgccgat tttccggcgg cggtggcact
     5281 ggctgaggcc tgcccggcac ttgttgacgc ggcactggca gataatgcag ccgctgtttt
     5341 tgaacctgcc gcagctgagg cgctctgtcc cgctgctgtt tcagaagact tagcgttcgt
     5401 ctcggacgtt tttgccgcct tcgcggaatt tcctgccgcc gttgccgagg aagctgcact
     5461 actggcactt gatgatgcat tcgtttctga agatttcgct gcctcttttg aggccgccgc
     5521 accccgtgcc gaggtggcag cttctgacgc cttcgtggtc gctgtggatg cagaagtggc
     5581 tgccgatttt tgtgatgctg cggcattcgt ttctgacgtt ttcgcggcac tggcgctggt
     5641 agctgccgcg ctttttgatg actctgcagc agcacttttt gctgcttcac gggcctttgt
     5701 cgatgccgtt cctgcgctgg aagacgctga ctgagccgac gacgcggcct gtccggctga
     5761 cgtgctggcg gcacgtgctg aggctgcagc atcggttgca tgagttgccg cctcgctggc
     5821 tgattttttc gcggctgccg tattctgtgc aaccgcggag gcgttacgtg acacctcttc
     5881 caccatctgc tcaaagcggc gcagtgcctc cggtcggaca tcatcctccg tcatggcacc
     5941 gagaaaatca ttcagcgtac ctggtctgga accttcatag acggtaatgg tcccggcatg
     6001 tgaaggcgga aaaccttcaa ccagcagggt gacgctgtac tgaccatgct caacatccat
     6061 gctgtaacgt ccggcttcat ccggattttc agaggccacc gtgttcacca ccatcgtgct
     6121 gctggttcgt ctggccttca gcacaatggt gcagttctgt actggttttc ctgtgccatc
     6181 tttaagcacg ccagaaattt ttactgtcat acttttccac caataaaaaa agcccgcagc
     6241 agtgacgcca cgggcttcag gacagtgtaa ctttacgttt cctcaaacgc agttcacccc
     6301 ataaggtgga tgaacctgcg tatcataaca atatttacag aagataaatc ggcgtctgtt
     6361 gtcagatacg gtatccgata ccaacaataa atgcatccgt tcgccagtca ccactaccgg
     6421 aaccttcata agcaaggtca atggtcacgg attcggtcgg gttaaactgc acgccagccc
     6481 cccacgccag agacgtgttg ctgtggcgac cgtcatcact tccggtcagc acatcgtgcg
     6541 ttttcccctt gttgtcagtt acgcggagat aatccccgga gaaagtcgac acacggctgt
     6601 aagccacacc caccatcgca tacgcgctga accattcatt cacgcgtaca gacggccccg
     6661 ccatcacgct gaaccagcgg ttacgcacgg aatcttcatg ccagcgggta tcgctgtagt
     6721 gcgttttttg ctcatcctca gcattggcat aactgaagga cgtaatcagc cccagcgcgt
     6781 ccgtaaactc ataacggtat ttcacgttaa tcccgttcag attatcgctg ccgggagcgt
     6841 tcgtacgggc atgaagatac cccgcgctca gtgtggactg atgttcagac gcccatgcag
     6901 gcgcaccgga tacggacaga cagatggctg cggacaaaat ggctgcacaa actttacgca
     6961 taattacctc tcgcttttct gcaataaaaa aggcgccatt tctggcgccc gtatatgggt
     7021 tataaaattc agctgatact gatgcctgcg gtggctttct tcatcaccac aaccagcaaa
     7081 tcgctgatac ttgctgtggg ataccagtta ttcaccagcc atgctgacac cgaaaactcc
     7141 agcgtcatgt ggccgcgacc agctggcata tcaataacac cactgtaaat cagcgtatta
     7201 tccagcgcgg tacggttata aatttcagca ccgtttttcc gtactatcag gcggcatgac
     7261 gaataaatat cgttattctc ccgctcatgt ttagcgccgc tgaatgccac cgccggaata
     7321 acaatttgcc tgtcaaaagg ctgatcgtca taaatcctga cggtaatggt ccctgatggc
     7381 caccgctccg gtgcccggga gtcccgcgga aaagccttac ccactgtttt gactatatcg
     7441 ccttcaatct ggttggctga cagtttcccc ttaatctgac agttctcatt aattgtgaca
     7501 ttgttgagcg tcccggcgtt cgcattcaca ctgccactga tatctgcatt tttagcggtc
     7561 agttttccgt ccggtgtcag ggaaaatgcc ggaggattac cgccgctggt aatggtggga
     7621 gccgtcaggc gtttcaggaa cacgtcgttc atgaatatct gatcgccctg accaacaaac
     7681 atcggctttg tgttgccatt cgcaggatta atcatcgcaa tcctgtctgc cgccagcagc
     7741 acctgactct gcattcctgc tggcgtattc tcaataccgg caccgatacc cgcaatataa
     7801 aggcgtccgt ccttcatctg ttgcagcttc acagcccaca tgctgttcag attattattt
     7861 gtatcaacct gaaccttctg tatctgctgg attgccgcac tctggtcttt cagtttctta
     7921 ttgacagtct gcgtgatttc attgctgaca tccgtaatgg acgtcctgat ttcagccagg
     7981 tcaggcgcaa gctgaccgtt atcaatctgc gtccacagct cctgagccag atgtgttttc
     8041 cctatctctc ctttgaaaaa atccaagtaa ccttccgcat catcgctcgc ccgaccgaca
     8101 gcctccacga atgccgattt gccaacggtg ttcacactgc gaacgtaaaa ataataatca
     8161 tggcccggtt tgatattgat actggcggct atccagtaca gcgccgtgcc aagatagcgg
     8221 gcgctggttt caacctgcct gatatccgca atccgctttt ccgagaacca gaactcaaac
     8281 tgtaccgtcg ggtcataaac ggcaagatgc ggcgttgcgg ttatctgaaa atagcccggc
     8341 gtcagctcaa tccgcgacgg tgctgccggt gcggcaatcc ggaaggtggt ggtggcaggt
     8401 tcaccctgct ggccatagct gtttatcgcc cgcaccgtca gggtgtattc cccgagcggc
     8461 aggccgctga aacggtgctc cgtgtctgcg gtgatggcgg tggtcaccag tctggcatcc
     8521 gttcccttac cactggtcag gcgcagactg aagcgcacac ccttcaccac ccgcggcgtg
     8581 tcccatttcg cctgcgccag atactggccg tcaggtgctg cactgccggt gggatgacgc
     8641 tgttcaggga acctgactgc ggctcaaagc gggcaccgtt atccacgatg gcttcttttt
     8701 ccagtacgtg ctgcaccgcc gtgatggcaa aggtgccgtc cgtgttttcc cggacggaga
     8761 cacagcggaa caggagacgg cgcagtgacg gcagggagag tccccacacc atcaggcagg
     8821 gtactgacct gtatccggtc cggcgcgggg tgttcggtga tgtccacact caccggctta
     8881 ccgctgccgt taatcaggtt caccgtggcg gcaccggtct ccggaagtgt cacttcacgg
     8941 tccagcgtca gagtgcgggt ggcagcatca atggacagga cacgtccgcc ggtcagggtc
     9001 ccggcatagt cgttatcaca gatttcaatg atgtcaccgg gtgtgtgccg cagcccctga
     9061 gacccgagcg tgaaatccac cgtctgcgtt tccagcagtt cggtctttat cacccacagt
     9121 ccggcacggt gggcctgacc gcggctggta cagccgaacg cgtccatctt cagcaggttg
     9181 cgtccgtagc gcagtatggc ttccgggtct tccaccagtt ccgtggaggt ctgccagccg
     9241 ttctgcgggt cggtataatt cacctccacc gccgtgtgcc ggtccttcag ggcactgaag
     9301 ctgtagcgga aacccacgcc gttatcatcc accaccacat cgctgttggt gtacggccac
     9361 accacatccg acgggcggtc ctgaacaaac gtcagcgtct ggccgttcca taccggcata
     9421 cagcgcatcg ccgagcagaa atcactgagc acgtcccatg ccttacgctg ttgtgccagg
     9481 tacgcattga aggtcatccg cggctcggtc tccccgaaac tatccgggac cgtctggtcg
     9541 cagtactgcc cgatggcata cagcgcccac ttgtccacat ccgccgcccc cagacgtttt
     9601 cccatgccgt agcgcgggtg agtcagcatg tcccacagac accaggccgg attgttgctg
     9661 tatgccggtt tcaggctacc gtcccagata ccactgtacg tgcgtttttc cgggtcatag
     9721 tttgacggca cctggatgat gcgaccgcgg atatggtagt tcaccgtcat ctgctggccg
     9781 ccgaactgct ccgcatccac ctgcagcccc acaatggccg tgttcgggta gcactgtttc
     9841 acatcgatga tttcggtgta tgacgaccac agcgtcttat tctgcagctg gtccgtggtg
     9901 ctgtccgctg tctcccggac catccggatg ttaaaaggac ggggaggcag attatccaga
     9961 atcaccgacg ccagaaactg cgaggtggtc ttgccgttaa tggtgacatc cttttctgtc
    10021 acccagttac cgttacgctg taactgaatc agcaggcgga cggatgtcgg gtttcggtca
    10081 ccctttgacg tggtctgcac cagtgactgc accccgaagg taacccgcag gcggtcaatg
    10141 ttcgcggacg taatggtgcg cgtcaccggt tttgccttcg tcacttccac gcccagtccg
    10201 gtttcagcac cggaggactc aaagccttcc ggtggtgtct gctcctgctc cccggcgcgt
    10261 cagaccgccg tcacaccgtg tatcacggga ttaccgtccg tgtccgtcag cggggttttg
    10321 ttcaccagga tactctgcag tcccttcacc ggaccttcta tcagtccctc accaatcgca
    10381 tcaatcacgc tcatcatctg cgtggatttg agattatcct tcgcctcacg aggcgtgtgt
    10441 gccttaccgc caccttttcc catacagcct tcccctgaat aaattaaccg ccacttgcca
    10501 ttccgtacag aagtcggata tccttcgccc gaaaagcatg aaacacattt ctgccatgct
    10561 aaagagaaac cccggtatca gcagataccg gggttttctt tcatgcccac cgataatcct
    10621 gttggttaaa accggtaatg gcataaaaat tctgaatatc ttcacatttt cacaaactga
    10681 ctgtggcgcg tataatttct ctgcgttaat ttttttgtcg tgatataaga ataattcctt
    10741 acacttaatc ttcgtaactc tcccgcagtt cctgtccgcg atcactgcgg gattttttta
    10801 ttctttttac ccctgccgcc cgataaccac gacctttccg cccccgcctt catcacgggt
    10861 gctgatgtcc tgggatatac ggcgggagcc aaccagcatt tccccgtaag gcaccggcat
    10921 cgggttcccc tgggcaatca tgttatccag tgaggaaaag tacgtgttct gtctgccgtt
    10981 atccgttgcg cggtaatccg gtgtttttgc cttcggggcc agcatctggg ccacaccgcc
    11041 cagaatcatg ctggctccaa gtgaaaacag catcgtggtg gcagaaaaac caccggctgc
    11101 cagggctgaa ccccataacg ccattgatgc cccggcagtg aagaaagagc ccacgatggc
    11161 tgccgccccc agcacaatct gcagtccacc ctttccggcc ccggccagtc gcggcacaat
    11221 gtggatgacc gttccctcac ccagctgttc gtgaagacgg gcgtacaccg cctccggtgc
    11281 cgtgtcataa ccggcaatac gtatctggta ccagccttcg ttcatctgac ggcgaaagcc
    11341 cggcatctgc atcgacaggg cgcgaatggc ttccgctgcc gtgttcacat acaggctaag
    11401 gcggcggcca aatcgttgta aatccccgtg aaggcagatg cgtgccagtg gcggtgacgc
    11461 cagactgaat gcgttcgtcg ttgccatttt tcggaatacc tctcccgttt actcagttgt
    11521 tcaggcagat ggtgaagcag ctcaccgttg ccgcagtaaa tggcggcatg gttcggtacc
    11581 gaagcaccaa agcagcacag cagaatatcg cccgcctgtg cagaggacag gggcacccgg
    11641 taaaagccgg tgaccgccat attgtccagg taaaggttct gaccgttgcg ccaccagtca
    11701 tcctcgcgat gaaaatccgg catttcagtc cccgccagat gataagcatc ccggaacagc
    11761 gtgtaacagt ccgtcacccc gtgctcaaag cgccgtcctg tcagatgtgg cacacagcgg
    11821 aatttgtgaa tgtcaccccg gcagaccaac caccagggca gtgcgctttt tatctgcaac
    11881 cgccggtcag cctcgctcag ccagggcagc ccaccgggat gactgtggac cagtgccaca
    11941 atctccccct gcatctctgc ccgcagccag tcttccggtg cgatacgaaa atacgcctcc
    12001 ggctctgcgg aaatattcac acaagggata taccgctccc cctccggcgt gcttatcacg
    12061 aagccgcacg actccgcagg cgcacaccgc cgggcatgtg ccagaatcgc tgattcagtc
    12121 tgtgtcataa accgggattt actgcgaaag tttattaatg gaaaggaaac cgccaaaatt
    12181 gccgacattc ctgcgcagtt cacacccgcg catgcacttg ctgcatctgt ccttacggat
    12241 atccgtggtg gggttgtcga actcatccgc caccgcagga ccgttatacc cgcattcatc
    12301 tccccggtaa tcccacatac aggtgttcgc cagcatgatg cgaccgggaa acagcgcccc
    12361 gtccgtctcg gtcggtgtag ccagcacaaa cgaggccgtc atggctgtca gctgcgacat
    12421 ctgctccacc acccagcggt cactcagctc ctgctccggg tccgcctccg gattgcccgc
    12481 aacgaaattc accgcatcca gaaaacgggc atacacccgg cggcggacca ccgtggcccc
    12541 caccagactc tgcaggtctt ccgccatccc ggtgaccaga ccgaacagat tggacaccgt
    12601 cagcgacggt ctggcactgc tgccccggcc gttcatctca aagccgctgc cgtcaatcgg
    12661 gtatgcctca tacttacgcc cctgccaggt gaccggctcc cctttttcat tcagctcatt
    12721 acagaaaaaa taccgctcac caccctgtac cgtcaggtcg atttcccaga gtaccacccg
    12781 cggtgactgc tctgacttaa ccgactcgtt cagactttct tcgcgaatat cctgcatcag
    12841 ttcaccacct gcttaaactc cgcgctgaac tcaacgcgca acatcccgac ccgcgcagac
    12901 cacccggcac aggtcacctt tatctgccgg tatgcatagg gtggcttcca caaaaatgcc
    12961 ttccagccac cgtgctctgc caggaacgct tccagatgcc gggcctcctc ccgggtcacg
    13021 gaaagcgtca ccctgtatgt tttcaggtca gcattcagcc ctgccgccat acgctgtgag
    13081 tacccgtcac caaaacgcac ttcacgcacc gatggctgcg agttcacctc catatccggc
    13141 ttcactttcc agcgaaaggt tttcatccac cgctccctga taacataccg ccatcacgca
    13201 actgcagccg gagttcatcc tgtgccccct tgcgggccat ctcatacacc gctttcatca
    13261 gctgcggccc tgcccgcccg ttggggccgt cgttctgaat caccacgtga ttgttctgat
    13321 taaaattaat gccttccgcc cgccgcatct gcgccggact tccggcaccg ccgacataac
    13381 caccttccgc atagccccgc atcaggcggt acagattgcc gacaccaatc cggctggtcg
    13441 cctccttcgt gaagacaaac tccccgcgat gaacaatccc cgcaggttca tatttacccc
    13501 ccgtccccgt aaatcccccg gtcgcgaaat ggaagttcgc cgccgcagcc tgaatggccg
    13561 tccccgtgga ggcagacgca ccaccaccga aagcaccgcc aatggcgctg ccgatactcc
    13621 cgactatccc caccatcgcc tgcttcagaa aaatctctgt cagcatggac agcacagaac
    13681 gggtgaaacc acgccagttc tgttcgctgc cgatcagcat cgctgccata ttctgtgcaa
    13741 taccgtcaaa ggtctgcgtg gccgcgtttt taacctgcga aaaactgtcc gtcgcacttt
    13801 ccgcccactc gccccagccg gacttcatcc cggccatcca gcttccacga agctgctcct
    13861 ccgcagacca ggtgttcttc agtgcagatg tggccttcgc cagcgcagcc ggattatcac
    13921 cgtacacctc acgaaggcgc tgctcttccg actcccgctg cgcctgacgg tcggtgagtc
    13981 cgcgggcttt tgcgctgatt gccgcctgct tcgcgctctg ctgctgttca aaccgcgccg
    14041 cctgctgtgc cagctcattc agccgtttct ggtgttcaat cttgtcgccc agctcagcca
    14101 gctggcgttt gtactccagc gtttctttct catgggccag cagggatttt tcctgctcag
    14161 ataactgccg tttcgtggcg gcctctttca ggaccacata ctgattttcc gctttccata
    14221 aatcacggcg ctgctggctg attttttcat tcgcaccgct gtgcttctcc agcgtcctga
    14281 gctcagtttc aagcgccagc agggctgcat gcgcctggtc ttcctggcgc tcaccggcag
    14341 acaccttcac accggacggc tttttctgcg tcgactcata atcctttttt gccgacgcca
    14401 tcagcgtgtt gtaatccgcc tgcaggattt tcccgtcttt cagggcctta ttcagctctt
    14461 cctgccgggc ggtatatttc tccagtggcg tcagcaggcg ctcatacgcc ttctgcgcct
    14521 ctccggtata cttcagctgt gacgactcac gctcagccct gtcccttgcc gccagttcac
    14581 cggctttttc catatccgac tgcagcgttg ccgctgccag acccagacgg gcattttccc
    14641 ggtcatccca tgcgccctga aggttggccc ggaaagagga ggttttaccg cggcgctggc
    14701 tccgactctg gtaccactgc cattttttat ccgcctcatc aaatgccttc tgcgcactgg
    14761 cgagcatatc cgctgaggat tccggacgac cgatatccag aatggcatcc cacatcgatt
    14821 tgaatgcctt ccctgtttta tccgcccagg tctccagtgt tcccatgttt tctttcaggc
    14881 gacgggtctg ctcatcaaag cctttcgtgg cgatatcgtt cgccgcctgc aatgccccgg
    14941 cctcgtctcc ggaacgctgc agctgtgcaa catacgcaat ctgctctgcc gtcacgttac
    15001 ggaactggcg cgccatcgcc atcagtcccg acgtcgggtc agtggtcagc ttcccgaagg
    15061 cttcagcgac tttatccacc tccacaccgg atgcagacgc aaaacgcgcg acactctggt
    15121 tgatggcatc aaactgttca ccaccacgca caccggcatt caccagggct gccagtgact
    15181 ctctcgcctg gttaaacgtc agccctgctg cctgcccggc tcttgagaga gtcagcatac
    15241 gatcggcagt cagtccggac tgattaccgg aaagaaccag ggttttatta aacgctgaaa
    15301 gcgtggaatc tccctggtac caggcgtaca ccagtgcacc tgtcgccacc gccagcgagg
    15361 tgaccccgac catcggcagg gtgatcgcat cggcaagccc cctgaacatg gggatcatcc
    15421 cgccgaagga gtccttcacc tgaccgccct gttgcagcag gatcagccag ggattctgac
    15481 caccggcaag ctgcgtggcg atatccgtaa actgtgcggg cagggttcgc atggccgctt
    15541 tatactgccc gacggaaatc ccggcttttt gtgcagccag cgcctggcgg ctcaggccct
    15601 gttcaacagc actggcggtt tttctggcgt cggtatccag acctgaaaaa tgacgcctta
    15661 cccggctcat ctgctcatcg aaacggacag catccagact caggtcaata acaagatcac
    15721 caaccggctg ggacatatct cacacctccc ggaatccccg ctgaagccat cattaatgcg
    15781 gcatcatcca ccatgacatc cgccacatcc gcagacgata aaatatcgcg ccctccgtcc
    15841 ccaccgaacc ggacgcctcc ggcaagtcct gccgctttct gcatcagcat tttgtcctca
    15901 tccggcctct ccacctgctc ttcctcatgc cgggggacaa gcagactgaa atcagaggga
    15961 tgcatatccg gatcgcaaaa aaacaggctg agtacagcgt acgtcagccc ggaaaaatgc
    16021 atatccagct gggtatcctg aaaataatgc gtgcggtaaa aacggcgcca gtcggcatat
    16081 tcggtggatg tcatcccggc aagcatggcg cgccagtcgg gtctccccat ctcacgcgcc
    16141 agtctgaggg caaagttcag ctcgccgtcg aagactttcc cgcagaaaaa tcatcatcag
    16201 tcagcgtgtt atttttcgcc acttcagtaa tatcagtatc cggacgaaca gcttcgatca
    16261 tcccggacag gcacaacacc acgtcttccg cccgggcaat ggcatcggca ggccaggtgg
    16321 tgagcacttc ctgctctatc ttcatcacgg cctcattcat tgacggtgac tgcgttttct
    16381 gtggatggtt atgccacagg gacatcgcca ccagaaacgc gccggttctg acgagatctt
    16441 ccacgcttac ctgcaggttg ccgctggatt ctgcctgttc tgcacgccgt ttcaggaggg
    16501 caagatgctc gatacgctgc agcgcagaca attcggaaag cgtgacagac acaccgttat
    16561 attcaaattg ttctgttttc agaaacatgt attacctccg tttaccctgc agcgcccgct
    16621 tcagtaacgg tgacttcagc cactgcggcg aactgaccat ttccgctcac cacagggatc
    16681 tgcaccttac ctgtcgccac gccgtttacc gtaattgtca tatctttcac actaatggtg
    16741 gctttcgacg gatcggcgga aaccgctctg aacgtcttgt cggttgcact ttccggctca
    16801 aaagaaaccg tcagggtggt tgttttccct tttgccaccg taccggatgt cggcgtcacc
    16861 ttaatcgcac tgaccggcgt aattttgctg cgttcttccg ctacagaagg tttacccacg
    16921 ttagtgactt tcaccgtgcg ggtgatcact tctttcgccg tcacggcctt accgatactg
    16981 ctgacccagc cacgaaacac atccaccgtg ccattcggaa aacggatttt ataggcccgg
    17041 acatcgccgc tttcaaacca gcctataagc cctttctggc cttcctctcc cggtttccag
    17101 gccagcgtaa aactggtatc acctgcagat ttctgcccct gcccggtcgc gctccagtcc
    17161 gcgtcttcat catccaggta gttatcatca taggattcag ccgtcatctc gcccggcgtc
    17221 agatccttca ccttagccag tcgctgccag tcatcgtctg acaacgggtt tgcataagca
    17281 tcagccttgc cgttgtaaac ccacagagtg gtaccggcac cttttaccgg ctccagggga
    17341 tttggtgttg ccatatcgtc ctcacatctc gtatgtaatg gaataagtca gatccgcaga
    17401 gctccataac gccatatcgt catcacgacg atactcatag ccctgcgtaa ccatcgtggt
    17461 aatcagtcct gccagtgcag ggatcgcagt catcgccgga taaatccggc tttccatcca
    17521 ctgatcgagc tccgaatcag gtacctgtgc cggtaaaaac acctcaatat gcagtgtggc
    17581 ccgccaggta tctgcatcca gctcttcacc ggtatactct gcatccgtca gataaaccgc
    17641 gatcgcagga aaatcctctt cgtcaaaaac aacggggcga ccatcaaaca gcgtcgcccc
    17701 gtgttcatgc tgctcgagtg catccagcac tgcggcacga atgtcagtgt gtttcatcgt
    17761 tttatcgcaa tcctcagttg ttgtttcagc gcgtatgcca gttctttagg caggcgttca
    17821 cgccggatac ggtcaacatt ctcatcaaat gcctgtttca gtggggccgc catcgggatt
    17881 ttcaccacct gaatgggaag gcgattacgc tttttccttc ccttgtcgtc attgccctcc
    17941 tcatatctgg cctggggaag acgttgcata acatgccagc gcccattatt taatcgctgg
    18001 ataaatgccc gctgataacg atgctgaccg gctttgagta tgctgttcgg acgacgcccc
    18061 agcattctga tccccagctt aatcacaggg agatcaccgc ggttaacgat aattctggca
    18121 ttcggatttc tgaccgtcgc ccgtttcagt ctggaccgtt cctttaccag tttccgtctc
    18181 accctggttt cccgggcaac ctgtgacgaa gactgattaa tcgccgttgt ggccacgcgg
    18241 ttaatggtca ttgcagaagc agccggaatg gcgtttttac gaacccggtt cagattgtca
    18301 atcgcctgat caagcccttt tatcgccata attcaccctg cgtttatcgt cgccggttaa
    18361 ctgcgggtgg ttgcccacgg ttgagccaga gataacagct gcccccgtca tccgg
//
LOCUS       DAZRUF010000066        18351 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZRUF010000066 DAZRUF010000000
VERSION     DAZRUF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.066"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(148..1257)
                     /locus_tag="ACHXOL_001813"
     CDS             complement(148..1257)
                     /locus_tag="ACHXOL_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR6808810.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRAEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPSKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLAHGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            1415..2599
                     /locus_tag="ACHXOL_001814"
     CDS             1415..2599
                     /locus_tag="ACHXOL_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR6808811.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLPRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHDCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVIGAIRAKLDSLVTAG
                     KLIGASCWYDVVDNGTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            2599..3111
                     /locus_tag="ACHXOL_001815"
     CDS             2599..3111
                     /locus_tag="ACHXOL_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR6808812.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGRDIVP
                     DRIRSALGLG"
     gene            3166..3531
                     /locus_tag="ACHXOL_001816"
     CDS             3166..3531
                     /locus_tag="ACHXOL_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808813.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGEKNEQATEA"
     gene            3540..3695
                     /locus_tag="ACHXOL_001817"
     CDS             3540..3695
                     /locus_tag="ACHXOL_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR6808814.1"
                     /translation="MCFEHIEDFVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            3682..6489
                     /locus_tag="ACHXOL_001818"
     CDS             3682..6489
                     /locus_tag="ACHXOL_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR6808815.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTGQYNNQLERERRALARVT
                     QARERYSRAQETAGKLKTGGALATGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLAKMGIRGSDAGTALRKSLANLSSPPKAAAEALKELGVSVADA
                     KGKMRPMENVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMGLVDAAGDGFLAKLR
                     KELEGARGEAERTAKVMANNLDGNLKSLGSAWEGLRIRISDLVDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLATTATVGSLSLVIGVLHGKLATLRLGFSLLTES
                     MNVVRVLPALWGMVTGSVSLLGGAIGALFSPVSLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDVVRDGIIQVFNWFKSLLSPMESSKETLDKCASA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            6502..6990
                     /locus_tag="ACHXOL_001819"
     CDS             6502..6990
                     /locus_tag="ACHXOL_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808816.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDERIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(7026..7613)
                     /locus_tag="ACHXOL_001820"
     CDS             complement(7026..7613)
                     /locus_tag="ACHXOL_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR6808817.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGISTIYRYHPAGESIGTIEKSQETK"
     gene            7895..8638
                     /locus_tag="ACHXOL_001821"
     CDS             7895..8638
                     /locus_tag="ACHXOL_001821"
                     /inference="COORDINATES: protein motif:HMM:NF024018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR6808818.1"
                     /translation="MCKGSWAYANNKVITDTGCGNICLAGAVVEVIGTRGAMTIRVTT
                     PSTSSGGGITNAQFTYINHGDAYAPGWRRDYNTKNQQPAFALGQTGSTVGNDKAVGWN
                     WNSGVYNANIGGASTLILHFNMNTGSCPAVQFRVNYRNGGIFYRSARDGYGFEADWSE
                     FYTTTRKPSAGDVGAYTQAECNSRFITGIRLGGLLSVQTWNGPGWSDRSGYVVTGSVN
                     SNRDELIDTTQARPIQYCINGTWYNAGSI"
     gene            8641..9174
                     /locus_tag="ACHXOL_001822"
     CDS             8641..9174
                     /locus_tag="ACHXOL_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR6808819.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGVVI
                     KRTYTGEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(9203..9730)
                     /locus_tag="ACHXOL_001823"
     CDS             complement(9203..9730)
                     /locus_tag="ACHXOL_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR6808820.1"
                     /translation="MMHLKNIKAGNAKTVEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDANNIIVAITKDASTLNPEGYSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(9732..11768)
                     /locus_tag="ACHXOL_001824"
     CDS             complement(9732..11768)
                     /locus_tag="ACHXOL_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808821.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQATAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVTLDATHQNHIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPTVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVNYVDDKL
                     KEHEQSRRHPDASLTAKGLVQLSNTTNSTSETLAATPKAVKAVMDETNKKAPLNSPAL
                     TGTPTTPTARQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATT
                     MTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAV
                     IEYLGLQETVNKAGNAVQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNNSDADTD
                     SYMWFETGNNGNEYFKWRHRIIGTRPKDLMNLKWNALSVLVEALFSSEVKISTVNALR
                     IFNSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLDVTGD
                     IFANRFAINSSTGMWIHMRDQNVILGRNAVSTDGAQALLRQDHADRKFMIGGLGNKQF
                     GIYMINNSRTANGTDGQAYMDNNGNWLCGSQVIPGNYGNFDSRYVKDVRLGSQQYYGV
                     NNWQTWNFQCPSGYVLTGINVQDTGKNSADNIAGVHYRPVQKYINGTWYNVASI"
     gene            complement(11771..12301)
                     /locus_tag="ACHXOL_001825"
     CDS             complement(11771..12301)
                     /locus_tag="ACHXOL_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR6808822.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPADLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFNGEPGTFKIDVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIQGDIFA
                     AAATYDGEVITIYPDD"
     gene            complement(12294..13190)
                     /locus_tag="ACHXOL_001826"
     CDS             complement(12294..13190)
                     /locus_tag="ACHXOL_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR6808823.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIAAYPAEQREVVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEAARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDVVMENDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            complement(13194..13544)
                     /locus_tag="ACHXOL_001827"
     CDS             complement(13194..13544)
                     /locus_tag="ACHXOL_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR6808824.1"
                     /translation="MTTRYTGMNPDGTGNLNDMAHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSATVIALTRWEPRIALNAIDVVWKAGGHAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            complement(13541..14122)
                     /locus_tag="ACHXOL_001828"
     CDS             complement(13541..14122)
                     /locus_tag="ACHXOL_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR6808825.1"
                     /translation="MSAELMRLLSNIIRTGIISEVDEESWCVRVRSGELETGWLRWNT
                     TRAGDFNVWFPPSPGEQVAIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDG
                     AVFRYDADAGALSASGMKTATLQASISVTLNTPVVECTDLLRTATLDVTKGGTMSGNI
                     THSGGDFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(14119..14754)
                     /locus_tag="ACHXOL_001829"
     CDS             complement(14119..14754)
                     /locus_tag="ACHXOL_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR6808826.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTRRVSMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(14747..15214)
                     /locus_tag="ACHXOL_001830"
     CDS             complement(14747..15214)
                     /locus_tag="ACHXOL_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808827.1"
                     /translation="MLKTDSLREAMTRSCRWCHANPEKFTIFVESGNIETTGETPSFV
                     YRYQIVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(15286..15759)
                     /locus_tag="ACHXOL_001831"
     CDS             complement(15286..15759)
                     /locus_tag="ACHXOL_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808828.1"
                     /translation="MKVRYILMMALTVLTLLTITLWQTFRLGQEDGHNRYTQAQLDDY
                     SRTLKEAGTIISTASGVLRDVMTARQNRQQEGEIRREQLRNDITTDACARAVPDARFT
                     ERLHQHAERVRAAAVVPFHPGRADRPRTSPAVSGDAIMGKHRDMERQPHGRTGNL"
     gene            complement(15756..16304)
                     /locus_tag="ACHXOL_001832"
     CDS             complement(15756..16304)
                     /locus_tag="ACHXOL_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR6808829.1"
                     /translation="MIKLPVKRYAVAAIVALGFSIAPHKLRTSPEAQIRIATWEDCRA
                     TPYRDIAGVMTVGCGSTGHVENRLYTETEVAGRWVNDMQHAENCINQNFSGNAMPQSA
                     FEAMTDAAFNLGCRNLMWFRDKNRNYHRTTIWKHAQAHNWPDMCNRLTDFVNSGGERS
                     QGLVNRRTDFRAWCLRDTGGQS"
     gene            complement(16285..16587)
                     /locus_tag="ACHXOL_001833"
     CDS             complement(16285..16587)
                     /locus_tag="ACHXOL_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6808830.1"
                     /translation="MVTGFFQRVSDWITFWASVTATTIGVMTISEKIALAGLLLGSLS
                     ALHAWRHRRLMETAMQRRNELIASILAQSDARPLHDSERQALNILRDNDTHDKAAR"
     gene            complement(16581..16778)
                     /locus_tag="ACHXOL_001834"
     CDS             complement(16581..16778)
                     /locus_tag="ACHXOL_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR6808831.1"
                     /translation="MKVKTLEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCRQVFLEA
                     GQEVEMPEPEKKKQREMIQLW"
     gene            complement(16778..17272)
                     /locus_tag="ACHXOL_001835"
     CDS             complement(16778..17272)
                     /locus_tag="ACHXOL_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR6808832.1"
                     /translation="MSMVARTEPGPAEDDITDTEDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(17374..18174)
                     /gene="gpM"
                     /locus_tag="ACHXOL_001836"
     CDS             complement(17374..18174)
                     /gene="gpM"
                     /locus_tag="ACHXOL_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFR6808833.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKADIKKALLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYAVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSVFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLSDADSMASSLKLLREAMHLNQNAGVKREIATLS
                     RALKKADSAAAPEDASAQQAQDESSESKKTTRKPATRKTTATQKAKRG"
     gene            complement(18220..>18351)
                     /locus_tag="ACHXOL_001837"
     CDS             complement(18220..>18351)
                     /locus_tag="ACHXOL_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFR6808834.1"
                     /translation="MRRLMKDEPEYNRIATYQSINDAYVVEDYGKCAMVTGLKFADS"
BASE COUNT         3974 a         4823 c         5044 g         4510 t
ORIGIN      
        1 acccctgatg tcaggtagga aaacaccata tgtttctgct ctgtttttcc ttagaaatag
       61 gcatccttgc tggaacaaag cgagcagcta tattgactct atgttgtgag tgtgttgatc
      121 cgttctgagt taatacattt aagttgctca ctctgtttct gctatccatt ccgggatttt
      181 tgcctcaagc tcaagctgcg tggtaaagcc gctgttatca atggtgtgct cggcttttgc
      241 aataatccag tcctgattat caatctcgct tttaaatcct gttaccgtgc catgcatttc
      301 ggggtagagt tctgcacgtc catgcgccag tgtgatggag aatgatgcgg ctccgcgttg
      361 tagctgctgc cactttgccg ccgctgcacg tcttgctgcc tgctcgttct gataagtttt
      421 gcgtaacaca aacacgttgc cttccgcacc ttccatatag tcgccttccc ggctgctgct
      481 tttctccttt tttggttttg acggtttgcg gcgtttcacg ctgacttttt tctttttccc
      541 gtaattaaga tcaagccagt aagcgcgtac ccccgtatac gcttcgcggt cagcaatgcg
      601 gaactgatgg cgatcgccgc tgctgcgtgt gatggcgaac gagggcaacg gctggccctg
      661 tgcgttcacg ccaccaccgg gcatgatgaa taacagatta ccgcttttta ccgtggtgat
      721 tgcgcccagc atttccgcca tgcgcgtaag gaaggacatg tcgctttctt cggtctggtc
      781 ggcgtggtcg atttcgatat ccatcagcat ttcgctgatt tgcggtttca gaccataccg
      841 atgagcgatg gcggatacca cacgctcaac ggtcacatca tgccaggaca cctcacgttt
      901 aacgttaaat tcagcccgaa aatctgcgct tctggctgaa acagtcagcc tgtccggcgg
      961 tccttcgtga gcgatttcat caacaatgta agtgcctttt tctgtcagcg gttctccctt
     1021 ccagccaatg agaaccgtca ggcgcgcgcc ccgtggcggt agctgcaact gaccatccgc
     1081 atcatccagc gtgatggtga gctggtccgc ctcaaatccc cggttgtcgg tcagtgacag
     1141 gctcatcagg cgctctgcca cgcctgacag cgttttaccc tccgcgagaa tatcaaaatc
     1201 cggcattttc acggggtctg tgccctgact gagcaattgc atggtggtgt cggtcatctg
     1261 ctccctccct gtgtggcatg gtcgcatgtg cgtgcggagg gggttactgc tttttgttgt
     1321 cgccgtggcg ggagaatggc gcaggggtga gattacgcgc gtggtgggtg atgattgttg
     1381 ccgaatcatt taacggatac aaggggttga agctatgagt gaaactcgtt ttcatggtgc
     1441 ccgtgttacg gaaaataccg acctggtaac agcgattaac gatgttgatt ccagcgttat
     1501 cggtatcgtg gcaacggcgg atgatgcgga cgcggagctg ttcccgctga acaagcccac
     1561 actgctgccc cgcgtcaatg acgtgctggg aaaatgcgga acaacgggga cgctttatcg
     1621 tgcgcttaag gccatcgcag accaggtgag cacaaaggtg atcgtcgttc gcgtggctga
     1681 acacaaagaa gaagacggaa aaacgcagga tcaactggtt atcggtggtt ctgaatctga
     1741 cggcagctat acggggatgt atgcgctgct tgttgcagag caggatgaaa gcatcggata
     1801 ccgtccgcgt attctggccg cgccggagct ggacacggag gcggtgacaa aatccctgtg
     1861 cgtgattgcg ggtaaactgc gcgcgtttgt gtatgcctca tgtcatgact gtaacacgat
     1921 ggctgaggca attacctacc gccagaaatt caacgaacgt gaggtgatgc tcttatggcc
     1981 ggacttcatc gcctacaacc cgaaaagtgg caaaaacgaa acgttccccg cgcctgccta
     2041 tgcgtgcggc cttcgtgcgt acattgacca tgagcagggc tggcacaaat cgctgtccaa
     2101 cgttccggtt aaaaatgtgc tggggatgtc caggcatgtg ttctggtcgt tgcaggccga
     2161 agacagtgat gccaacagcc tcaacaacaa agaaatcacg accattattc gtcgcaacgg
     2221 gttccgcttc tggggcaacc gcacaccgga aacgaacgcc tacatctttg aggtgtatac
     2281 ccgaaccgca caggtgctgg ctgattcaat tgcggaagcg cagtttgaaa ccatcgacag
     2341 tccactgacg cctgcgaacg tgaaggatgt tatcggtgcc atcagggcaa aactggattc
     2401 actggttact gccgggaaac tgattggggc gtcgtgctgg tatgacgtgg tggataacgg
     2461 caccacgaat ttacgtcagg ggcgcgtgcg tattcgctac aaatatacgc ctgttccccc
     2521 gctggaagac atggagcttt accagtcgtt tactgatgaa ttctttggtc ccgcatttgc
     2581 ggtgctggga ggtgcctgat ggctgtacca aaacatcttc gcttttttac gctgtttgtg
     2641 gatggtgaaa acgaagtggg taaggtgacg tccgtcactc tgcctaagct gacgcgcaaa
     2701 accgacagct accggggtgg tggcatgatg ggagcggtaa gtattgatct cggcctggat
     2761 gactccgcgc ttgatgcgag ctttgtcatg gggggcgcag ttcgtgagct gttccttaag
     2821 tatggcggca cgattgacgg tacgctgctg cgttttgcgg gtgaatacta caccgacgct
     2881 gaaagcgacc tgtatgaagt cgaaatgcgc ggacgtgtga cggaaattga tatgggggaa
     2941 gccaaacagg gcgaagccac atcacacact tacgccatta aaaacaccta ctacaagctg
     3001 agtgttaacg atcgcccgtt gtgggagatt gacctgctga acttcattta ccggaaggac
     3061 ggcagggaca ttgtgcccga tcgcatccgt tccgcgctcg ggcttggctg ataagtaata
     3121 tgcaggcggc gcagtgcgtc gcctctgact gaaaggagtt tcctgatgaa agagacgaaa
     3181 aacatcgata ccgaaaacac ggtcgttgct gacactgtga aagaaaccag tgagcgtggc
     3241 gtaaaactta cccaaccaat tgagcgaggc ggcgaaaaaa tcacgtatgt ggagatcacc
     3301 ggggctattg agcaggctgg atctctgcga gatttgtcgc tgtctgatgt gctgaatttg
     3361 aaagcggaat ccatgtttac gctgctgtca cgcgtgacat caccgcgact ggatgaagtg
     3421 acgatcaaaa aaatggcatc ccgtgacttt attcagttat gtgtggttgc cgtaaatttt
     3481 ttgagcggtg cggactctgg cgagaagaac gaacaggcga cggaagcctg atcacggttg
     3541 tgtgctttga gcacatagaa gactttgtgg cggatattgc cgccattttt aactggtcgc
     3601 ccgccgaaat cttcatgatg acgcccggcg aagtggttag ctggcgtgag cgggcggcac
     3661 ttcgcagcgg gaatgcagac aatgaagact cttgatatcc gggtcgcttt cagcgccgtt
     3721 gacaggctga cccggcctgc cgaaaacgcc cgccgcctga tggggcagtt tggtgactcc
     3781 atccagcgaa cgcagggggc gatcaaaaat ctcgagcgtc aggcgcgttc atttgagcgc
     3841 gcccgtgacg ctgtcagtaa agcggatgcg ggcatcgtga aagcacgacg ccagcttaac
     3901 gcccttaatc agttacaacg cacgggtaca gtgctcagcg aaaaacaaca aaagctgatg
     3961 cagcagttaa gcacccggct tgaacgcctg aatgaatcgc gcacacggga aattcagaaa
     4021 atgcgggaac ttggcggaga gctgaaacgc cacggcattt ccctgacagg cagcgataac
     4081 accatccagc aggccatcag acgcaccgga caatacaaca accagcttga acgcgaacgg
     4141 cgggcgcttg cgcgtgtaac gcaggcgcgt gagcggtatt cacgcgcgca ggaaacagcg
     4201 ggaaaactga aaacaggtgg tgcgctggca acaggtgcgg cggcagcggg cggctatgct
     4261 gccgggcgtt ttttgcagcc cgctatcggg ttcgggaaag agatgtcccg tgttcaggca
     4321 ctgacgcgaa tcgataaaaa cagcccgcag tttaaggcgc tgcgtgagca ggcgttaaaa
     4381 cttggctctg aaacgcagtt caccgcaggc gatgccgcca gtggacaggc atttcttgca
     4441 atggctggtt tcacaccgca ggccattcag gctgcgcttc ccggcgtgct gagcatggca
     4501 acggctggcg gtatggacct cggcgagacg gcggatattg gttccaatat cctgacgcag
     4561 ttcggcctct ctgctgacca gatggaccgg gtcggtgaca cacttaccgc agcgtttacc
     4621 cgtaccaaca ctgaccttcg cgcactgggc gaaaccatga aatatgcagg tccggtggcg
     4681 ggtaagctgg gaatatcgct ggagcaggcc gcagcgatgg cgggcgtgct ggcgaagatg
     4741 ggtatcaggg ggagtgatgc aggtacagcg ttgcgtaaaa gcctggccaa tctgtcatca
     4801 ccgccaaagg cagcggcaga agcactgaaa gagctggggg tgtcagttgc tgacgccaaa
     4861 ggcaaaatgc gcccgatgga gaatgtgctg gccgatctct ataaagccac ccgcaaatac
     4921 ggggaggttg accgggtatc gttctttaag gacattgccg gagaagaggc tttcacatca
     4981 tttatgggcc tcgttgatgc ggcaggtgac ggcttcttag ccaaactgag aaaagaactt
     5041 gaaggcgcgc gcggtgaggc agaacgcacg gcaaaggtta tggccaataa ccttgatggc
     5101 aacctgaaat cactcggcag tgcatgggaa ggtctgcgta ttcgcatcag tgatctggtt
     5161 gacggtccgc tgcgttctgt cacgcagtgg ctcacgcggg ttgtctcaaa ggtgacggcg
     5221 ctggcgcagg cccacccggc actgacgcgc cagttactga tagcgggagg cgcactgctg
     5281 gcgacaacag caacggttgg ttcgttgtct ctggttattg gggtgcttca cgggaagctg
     5341 gctacgttgc gtctcggttt ttctctcctg accgaatcaa tgaatgttgt cagggttctg
     5401 cctgcattat ggggaatggt gacgggttcc gtttctttac ttggtggcgc tatcggggcg
     5461 ctgttcagtc cggttagtct tatcgtggct gcgcttgccg gagctgccgt tcttatctgg
     5521 aaatactggg atcccatcag ggcatttttt gccggggtgt tcagcgggat tatggaaagg
     5581 ctgaccccgt tgcgcgaaac ctttgaacgg tttggccctg tttttgatgt cgtgcgcgat
     5641 gggattattc aggtctttaa ctggtttaaa tcgctgctgt caccgatgga gtccagcaag
     5701 gaaacgctgg ataaatgtgc cagtgctggc gagatattcg gtaacgttct tggcggtgcg
     5761 ttacaacttg ttctgacacc tgcaaaaatg ctactggata cgctggcgtg gatacttgaa
     5821 aaacttggcg tccttccgga tgaagcggaa agggcgcgca agaaaatcga agacgcacag
     5881 cgtgcggcca ttcttcagga caaggttgcc ttgcttcagg gggacctggc gaaaatcaat
     5941 ccgccgaagc ctgtggaaaa tggcaatggc accggaggtg ataaacccaa agacaacaaa
     6001 ccgctcacag acagcaatac cgggacgcta cgcagactca gcaaaattgc tgataacaca
     6061 ggtaagctgg ttgatgagac gaaaaaacgc attggccccg gcgatattgt ctttaagaac
     6121 ctgccccgcg cacttgctgt tcgtggggag tggcaggagc ggaagattgc gcaggtcagt
     6181 aagcctgccc ccgcaattaa tatcacaccc gtggtcccgg ctccgctgcc tccggcgctg
     6241 gtccctgttg ttgcggccag ctcccgcccg gtggcggagg ccatacgctc tccagtggca
     6301 tcagttcctg taacttcccg taaccgggag cctgttgcct ccggatttgg tggtgaaatt
     6361 catgttcatc tgcataacgt tgttacgcag aatccccgcg aactggcgaa actggtcggt
     6421 gaaatggtca gggcagaaat ggaacggcgc gcccgtgccg ggcgtggcag tttttacgat
     6481 aaagattgag gagtcatggc catgatgatg atctacggca tgtttgtttt tgagctgcgt
     6541 acattgccgc atcagcagtt acagcaaaac aaaagctggc ggcatgtgaa aaatgaacgc
     6601 gttaaccgtt cggcaagctg gcagtatatc ggtgcaggtg atgagcgcat cgttctttct
     6661 ggtgtgcttt atcctgaaat tacaggtggc gaagtgtcgc tgtcgctgct gaccacgcag
     6721 gcgtatacag gacgaccctg gcctctgatt gatggcgtcg ggcagattta cggcatgtat
     6781 gtcctgaccg aaacgaatac gacccgctcc gagtttgatc gctacggtaa ggcgaaaaag
     6841 atagaatttt cactgactct tgaacgctgt gatgaggatt tgcgggagcg cctgcaatcc
     6901 tcatcgttca gcgatatgct gtccggcttc aaagataagg taacatcatc ccttaacagc
     6961 gcggccagtt cagttaaagg gttgttctga tttaacacaa aaaccgctaa tggtcagatt
     7021 agcggttatt ttgtttcctg actcttctct attgttccga ttgattctcc tgcggggtgg
     7081 taacgataaa tcgtcgatat accaatgccg taaattattg ccagttgttt tctgtcgtgc
     7141 ccgtttttga tcagccttgc tatttgctca tgctgttctt ttgtcagctt cggtcgacgt
     7201 ccgcctgtgc gcccccgtgc gcgtgctgcc gccagtccgg ccagtgtacg ttcaacaatt
     7261 aattcacgtt ccatttcagc cagggcaccc atcacgtgga agaaaaaacg ccccattgga
     7321 gaagatgtat ctatgctgtc ggtcagactg cgaaaattaa ttcctcgctc ccgtagttcc
     7381 ccgacgagag aaatcagatg tttcatgctt cgcccgaggc gatccagttt ccagacaacc
     7441 agcgtgtcac ctttttgaag gcgctttaaa gcgcgtttta atcccggtcg gtctgtcttt
     7501 gtcccgctta atttatcttc aaatatttgt tcacatcctg cacaaacaag agcgtttcgt
     7561 tgcaggtctg tattctggtc atttgttgat acccttacat agccaatcag cacgctgaat
     7621 ctcccgtcca aaagcgcaaa tcatgccatg caggccggaa acggccatta tctaaaacct
     7681 cggtttacag gaaacggtaa atcaggcttc tggcgcatta cagaaaaacc agaacggcgc
     7741 agatattccg gggaaagata ccttcaccaa aaatattggt gcctgccgcg catatagctc
     7801 atggctgaat attggtggcg atagtcaggt ctggacaacc gcgcaattta tttcgtggct
     7861 ggagagtcag ggagcattta accatcctta ctggatgtgc aaaggctcat gggcttatgc
     7921 aaataataag gtcattacag atacaggttg cggaaatatt tgtcttgcag gtgctgtggt
     7981 ggaagttatt ggcactcgcg gcgcaatgac catacgcgtt accacgccga gcacgtccag
     8041 cggtggcgga attactaacg ctcaattcac ttatattaat catggtgatg cttacgctcc
     8101 tggctggcga cgagactaca acacgaaaaa tcaacaacct gcatttgctt tagggcaaac
     8161 aggaagcact gtcggaaatg ataaagctgt tggctggaac tggaatagcg gggtctataa
     8221 cgcaaacatt ggtggcgcat cgacattaat cctccacttc aatatgaata cggggagctg
     8281 ccctgctgta cagttccgcg tgaattacag gaacggcggt attttttatc gttcagcgcg
     8341 tgatggttat ggctttgaag ctgactggtc agagttttac accacaaccc gcaaaccctc
     8401 tgcgggagat gttggtgcat atacgcaggc agaatgtaac tcaaggttta ttacaggtat
     8461 tcgccttggt ggtctgttat ctgttcagac atggaatggt cccggatggt ctgacaggtc
     8521 aggttatgtt gttaccggtt ccgttaactc aaacagagat gaactaattg atacaactca
     8581 ggcaaggcca attcagtatt gcattaatgg gacgtggtat aacgcgggga gtatttaacg
     8641 atgatgcact taaaaaacat ttctgctggt aatcctgaaa caaaagagca ataccagcta
     8701 acgaaacaat ttaacatcaa atggctttat acagaggatg ggaaaaactg gtatgaggaa
     8761 caaaagaact ttcagcctga tacgttgaaa atggtctatg accacaacgg cgttattatt
     8821 tgtattgaaa aggatgtttc agcaattaat ccggaaggcg caagcgtcgt tgaggttcct
     8881 gatattacag caaatcgccg ggctgatatt tcaggtaaat ggatgttcaa agatggcgta
     8941 gtgataaagc gaacttatac cggggaagag cagaggcaac aagcggaaaa tgaaaagcaa
     9001 agcctgctac agctcgtcag ggataaaacc cagctatggg actcacagct acggctgggc
     9061 attatttccg acgagaataa acaaaaatta accgagtgga tgctctatgc gcagaaggtc
     9121 gaatccacag acacctccag cctgccagta acgtttcccg aacaaccaga atgaaacaag
     9181 gcccgatatc gggccttaat ttttactcag gcttttgtgg ccattctggc tttgccgtat
     9241 ccacacggct gaccagaaca ctgtagcgtt cccatgcttc cagtcgtgtg cgttcctcgt
     9301 ccgtcgccat attcagcctg acagcgcgtt ccagcggctg gatgactgat tcagcttcgg
     9361 aaagcaatgc ggccttttgt gattcggcct gttgttgctg ttcgtcagcc gtataaatcc
     9421 gtttaaccac agctccgtcc ttaaacatcc acttaccgga atcatcagca cgacgattag
     9481 ctgtaatatc aggaacctca acgacgctat aaccttcagg gttaagcgtg gaggcatctt
     9541 tggtgatggc gacaataata ttatttgcat cgtaaacaat ctttattgtg tctggctgaa
     9601 agttcttcac ttcctcatac cagttttttc cgtcctcaga gtaaagccag atgactccgt
     9661 gcttctttgt taactcatac tgctccactg ttttagcatt acctgctttt atgttcttta
     9721 agtgcatcat attaaatact cgccacatta taccaagtgc catttatata cttttgcact
     9781 ggcctgtaat gaacgcctgc aatattatcg gcagagtttt ttccggtatc ctgtacgtta
     9841 atccccgtca gtacatagcc tgacgggcac tgaaaattcc atgtttgcca gttgttcact
     9901 ccataatatt gctgtgaacc aagtcgaaca tctttcacat atctggaatc aaaattgcca
     9961 tagttgccgg gaataacttg cgagccgcaa agccagttcc cgttattatc catgtacgcc
    10021 tgaccatcgg tgccattggc tgtccttgag ttattaatca tgtagatgcc aaattgctta
    10081 tttcccagac cgccaatcat aaatttgcgg tcggcatggt cctgacggag caaagcctga
    10141 gcaccatcag tggataccgc attacgtccc aaaataacat tctggtcacg catatgaatc
    10201 cacatgccgg tactactgtt aattgcaaaa cggtttgcaa atatatcccc tgtaacatca
    10261 agaccatgcc ccatgcttat ccggccagtt ctgagattaa gcgtaaaggg gcgtagtggc
    10321 cctatattac cattttcccc ttcattctct cgtgtaggga tgatatgcag gcattcttca
    10381 gaacgacgaa aaatggcacc aaaagatgaa ttaaatatcc tcagtgcatt gactgtcgat
    10441 attttcactt cactgctgaa aagggcttca acaagaacag acaaagcatt ccatttaaga
    10501 ttcatcaggt ctttaggccg ggtgccaatg atgcggtgtc tccatttgaa atattcattg
    10561 ccgttgttgc ctgtttcaaa ccacatgtag gaatcggtgt ctgcatccga attattttta
    10621 aacccaatct ttgcccagtc agtatttcga atccaggcaa ggattgagtc gttttcaaaa
    10681 gtaagtccac cggacaacgt atcgccattt ttttgcacgg cgttaccagc cttgtttacc
    10741 gtttcctgta aaccgaggta ttcgataacg gcggcaacgg tcgatttcgc aagaatatcc
    10801 cgcccgactt ttgtcagggt tgccaggctg gcgacatcat tccccgtaaa atacggaaac
    10861 ctgtctgccg cagtagcaag ccccgccagc gccgtcagag tggcatcttt cggttgctta
    10921 cccgcaagcg cgttagtcat ggtggtcgca aaattcgggt cgttgcccag cgccgccgcc
    10981 agctcgttca gtgtgttcag tgcatcaggc gaagagtcta caagtgcggc aatcgcagcc
    11041 ataacgaaag ccgtgcttgc gatttgggta ttattcgttc cctgtcgcgc agttggtgtt
    11101 gttggcgttc cggtcagtgc cgggctgttt aatggggctt tcttgttcgt ttcatccatt
    11161 actgccttaa cggcttttgg tgtcgctgcc agcgtttcag acgtgctgtt ggtggtgttg
    11221 ctgagctgga caaggccttt tgcggtcagc gaggcgtccg ggtgacgtcg tgactgttca
    11281 tgctctttca gtttgtcatc cacgtaattc actgtggcca tcaccatggt gttatccacg
    11341 gtaagcgcca cagtggcagt gctggatacg gtcagaatgg tgcgaaatgt ttgtgcacgt
    11401 ccggatcctt cggcaacggt tggcttgtaa ctttcggcag tattgcctac cgcgatcaaa
    11461 tcgccgtgct catcaaacac accaatttcc cggatccaga atccgcccgt ttcaggagga
    11521 ataaccagct ccgcaataat gtggttctga tgtgttgcgt ccagggtgac gcgattaacg
    11581 gtatgtcgcc acacctcatg cacaagacgg gtctgcttac tgtctggcgt gggtaaagta
    11641 ccgccgccgt cacccacggc catatgtgtc aggcggacag gtttcccatc tggcgcggtt
    11701 gcctgcgcta atttttttgc acccgtatcg gtgataatgg ttttaaattt tcgtgttgtg
    11761 gtactcatgc ttaatcgtcc ggataaatgg taatgacttc accgtcgtaa gttgctgctg
    11821 ccgcgaaaat atctccctga atctcctgaa tgatattcag ccctgtcatg tggcggctga
    11881 ccgggcgggc atcagcaatc aaccgctcca tttccagata catttcctcc gtcacgccac
    11941 tgtccagcgt gccaacatca atcttgaatg ttccgggttc gccgttgaac tcccaccact
    12001 cagacacgcg aatgaggtat cccagcggct caatggcccg tcgcagggcg ctgatggttc
    12061 ctttgtgtcg gtgtatcagc catgcatcac gaatcacctg tcgctttgtc tcttccggcc
    12121 agttgcgatc ccagcggtca acggaaaacg cccaggcgag ataaggcagc agatgcaccg
    12181 ggcaggtgtc cggcgaccac agcgtgttga ggtctgccgg aatgtctgta atgcgtgttc
    12241 cgacggcttc ggcacaacgc atgaaattgc tggctgatgg tggtaacagt gaattactca
    12301 ttacgcccac cttcgctgat ggtgaatgac tcacagcgcg ccgcctgtat gtcgctgatg
    12361 gccatattct gtgtgggttc gattatctcc acgcgttgca caccgtgcac atgcagtgcg
    12421 gcagcaatgg cggacaacgc cacgtcctga ccgataagcc cctgctcagc cagccacttc
    12481 ctgaacgacg attcagccgc agccagaata ggttcggatt ccggaccggg gtaaaagtac
    12541 agttttgcat tcagccgcca tgtcacgatt ctggcactct gtacggtcag gcggtcggcc
    12601 accgggcggg tatcctctgc attcagaacg gcgcgaacgg tattaagcaa cgcctccgtt
    12661 gctgtgccgt cgccctcagt ggacaggatg gaaaccgtca catttgccgg agacggactg
    12721 atggcccgcg catcgcgcac cagaccgctg gcgctgcggg caaaatactc gtatgcgcct
    12781 gacgggcctg caacactcag gccgtcatac gcccgctgcg cccgcagtct cagcgaggtg
    12841 tcgttctcca tcaccacgtc ggtggtatcc gttgccggag tgataaccag gcgctttgtg
    12901 ttcatattgc ccgcgaggtt gtccaggtct gttccggcgc tgtggctcaa catgcaggcg
    12961 cgtgcggcct cattgacccg ctggcgtaac agcatttcac gaaacgacat ggtttgagcg
    13021 ataacgttca ggggttccga ttccagttcc agtgcggcgg agacgacttc acgctgttcg
    13081 gcgggataag ctgcaatcat catggccttt gtgtcagcca gaattgcctc aaagtcaggc
    13141 tccgcgatga tggcgggttc cggtaactgg gaaaggtcaa cggcaggcat gatttactcc
    13201 ctcagcgtga tggttaattc aacattctgc atggtctgca tgacagtgcc cgacagcgtc
    13261 accccggcgt ggcctcctgc cttccagaca acgtcgatgg cgttcagggc aatgcgtggt
    13321 tcccatcgtg tcagtgcaat cacggtagca ctcatgcatt gcagacgcgt ggtgttattc
    13381 atgggttcgt caatcaaatc aggcacaagg ctgccatatt cccgtcgcat aacccggctt
    13441 gccagcgggg tggtcaggat gtccctgact gactgtttca ggtgcgccat atcgttcagg
    13501 tttcccgtcc cgtccgggtt cattcctgtg tagcgggttg tcactgcggg cctcctgtcg
    13561 aatcgctgcc acctttaacg ccaccgtgct tatgcgtatg cactgtgatg ccgtttgagg
    13621 tgaaatcgcc gccgctgtgc gtgatattgc cgctcatcgt tccccctttt gtgacgtcaa
    13681 gcgtcgctgt tctcagaagg tctgtgcatt ccacgacagg cgtgttcagt gtcacgctga
    13741 tggatgcctg caaagtggcc gttttcatgc cgcttgcgct cagtgcgcct gcgtccgcgt
    13801 cgtagcggaa caccgcgccg tccggcgcgc tgaccacgat ttctttcagg cttttgccgg
    13861 gtgccggaat ggcatcactc cacaggctgc cgattatcat ggcggtttcc gggttgccgc
    13921 cgatgcaggc aattgccacc tgttcgcctg gtgatggcgg aaaccacacg ttgaagtctc
    13981 ccgcgcgcgt ggtgttccag cgcagccagc ctgtttccag ttcgccgctg cgaacgcgca
    14041 cgcaccagga ttcctcatca acttcagaga tgatcccggt gcggatgata ttgctcagca
    14101 gtcgcatgag ttctgcgctc atcgtacagc ctccgcaatc cggcccagca ccgtgttata
    14161 aatcaggcgc tcatctgcct ggctgatacc cagcagctca cgtaccgggt aatcggtgaa
    14221 aatgcccggc gcaacctgat cgcgctcacc gaactgatga acgcgtgcaa tacgtgcggc
    14281 cacgccgctg taacccaccg tcacaccgga agcatctgca cgggctttca ggtagcgggc
    14341 ggtgcgcagt tttacgaaca tggagacgcg cctggtgctg tcctggttga tgcgccgggt
    14401 gcgtatttcc agaaaacggt cgatgtcatc ccggtaaaac gtgcggatat tgtttttatc
    14461 ctcatcccac ccggtgatgg ttcgcccgta tttccccgtg tcgtgatgcc agtttttcag
    14521 cgtgcgtgct tcgttattcc agataaagcg aatgcgctcc tgtatccggg ttacgcggcg
    14581 tctgcgtggt gtccatgcgg tcccgtccgg cgctttctgt gaccggatac gcgcctgctg
    14641 ggcgcggcgt aaatcctgtg ccagctttct ggcgatgtta ttaatggcct gctgattcag
    14701 gctgtcgcgg atagcctcaa aggtttcatc cacgcgggtg aatgccttat ccatcgcttt
    14761 ccccccacgt cacatcctgg aatacatgcg accagtcgcc ttcggaagag ggcaggcggg
    14821 gttttggctc cggcaggtgt tctgcctgcg gtgtgccctg actgctgcgc gtgatgcgaa
    14881 cgcgttcccg cagggggagc gtaaacagga gatcggcgct gtcatcgtca ttgataacgg
    14941 cggaaaattt gatgtcctga ttacgctccg gattgagcaa caactgtggc tgattttcgg
    15001 ataaccacgc cagcagcggc agcgtgaggt cgtccagctc tccggcgtaa tccatgacaa
    15061 acatcactat ctgatagcgg taaacaaacg atggggtttc tccggtcgtt tcaatgttgc
    15121 cgctctccac gaaaatggtg aatttttccg ggttggcatg acaccaccgg catgaacggg
    15181 tcatggcttc acgcagggaa tcagttttca gcatggttgt tgtcctcgtt gttcagtcgt
    15241 tgcagcctgc gctgttccag taattcaatg gcccgcttat cggcgttaca ggtttccagt
    15301 gcgtccatga ggctgtcgct ccatatcccg atgtttcccc atgatggcgt caccggaaac
    15361 ggcgggggaa gtacgggggc ggtcagccct tccgggatga aacggaacga cggcggcggc
    15421 gcggacgcgt tctgcgtgct ggtgcaaccg ctcagtaaag cgagcgtcag gaacagcgcg
    15481 ggcacatgca tctgtcgtga tatcgttgcg tagctgttca cgtcggattt ccccttcctg
    15541 ttgtcggttc tggcgcgctg tcatgacgtc acgcagtaca ccggatgcgg tgctgatgat
    15601 ggtgccggcc tctttcaggg tccggctgta atcgtccagt tgcgcctgtg tataacggtt
    15661 gtgtccgtct tcctgcccca gtctgaatgt ctgccagagt gttatggtca gcagcgtgag
    15721 gacggtcagt gccatcatca ggatgtaacg cactttcatg actggcctcc ggtgtcacgt
    15781 aagcaccatg cacgaaaatc agtacgacgg ttaaccagtc cctgtgagcg ttcgccgccg
    15841 ctgttcacga agtcagtcag tctgttgcac atgtcaggcc agttgtgcgc ctgtgcatgt
    15901 ttccagatag tggtgcggtg gtagttacgg ttcttatccc tgaaccacat aagatttctg
    15961 catccaagat taaaggcggc atctgtcatt gcctcaaacg cggactgtgg catggcgttg
    16021 ccgctgaagt tctgatttat gcagttttct gcatgttgca tatcattaac ccagcgcccg
    16081 gcgacttccg tttcggtgta cagacgattt tccacatgcc cggtcgagcc gcaccccacg
    16141 gtcattaccc cggcaatatc ccggtaaggc gttgcgcgac agtcctccca tgtggcaatg
    16201 cggatttgcg cttccgggct ggtccggagt ttgtgagggg caatggagaa ccccagcgcc
    16261 acaatggcgg ctacggcgta acgtttaacg ggcagcttta tcatgtgtat cgttatcccg
    16321 cagaatgttc agagcctgac gctcactgtc gtgaagtggc cgtgcgtctg actgcgccag
    16381 gatactggcg atgagttcat tgcgacgctg catggcggtt tccatcaggc ggcgatgtcg
    16441 ccaggcatgt aatgcagaca gagaaccgag taacaacccg gcaagggcga ttttttcact
    16501 gatggtcatc acgcctattg tggtggctgt gactgatgcc cagaaggtga tccagtcact
    16561 cacccgctga aaaaaacctg ttaccatagc tgtatcatct ctcgttgttt tttcttctcc
    16621 ggctccggca tttccacctc ctgtccggct tccagaaata cctgtcggca gagtccgggg
    16681 ttggcatcca gcaccttttc ggtgacgccc tgcgtcgtgc cgtagtaccg gaaacagagc
    16741 gaatccacgg tgtcgccttc cagtgtcttc actttcatca gcacaactcc gcaaagattc
    16801 gcgggcggca cagaatgtca gagatggccc agctcacatc gcgccacaaa tccgatgtct
    16861 gtatatccag tgcgtccgcc cggcggtcgc ccttgtccgt tgtgtccgca tcgcggtaac
    16921 gctccagaat cagggcgcgt gtggcggtat aaacagcatt gcgccagtgc cagagattga
    16981 cgctttctcc gttaattaca ggtgccggaa catcggccag cgtctgatgg ccagccgcct
    17041 gctgttcctg ctgccatgct tccagctcgc gggtaacgtg tgccacggcc ccggtggcgg
    17101 tatgcagcag gcgggaggtg gtcacgcggc ccggcagtcg taccgccaga cgcagctcgc
    17161 gcagcacaat atccggccag aatgcacccg ctgaaatacg ggtatcgcca tcttcggtgt
    17221 cggtgatgtc gtcctctgcg ggtccgggtt cagttctggc aaccatactc atggggttca
    17281 ctcctgaaaa aatcgggcgg tgggtgcgcg gtgtaaacgg tcacggagcc aaaccggaac
    17341 accgcgcacg ccgcccgctg acggggtcag tcgttaaccg cgcttcgcct tctgcgtcgc
    17401 ggtggttttt cgtgttgcag gcttccgcgt tgtcttttta ctttcgctgc tttcgtcctg
    17461 cgcctgctgt gcgctggcgt cttctggtgc ggctgcggaa tcggcttttt tcagggcgcg
    17521 ggaaagggtt gcaatctcgc gtttcacacc tgcgttctgg ttcagatgca tcgcttcgcg
    17581 cagcagcttc agtgatgagg ccatgctgtc cgcatcgctc aggccacggc gggcaaaggc
    17641 gcacgccttg cataatttgg cgcgcacttc gtccggcata tcctggttgg cgacaatttc
    17701 ccaaagtgtg tccagtggtt cgataaagac ggacaaatcc gcgtcggcat ccgtcccggc
    17761 ctgcgtcagt accggattgc agatttcttc ggtcagcact gtggcagcag tacggccaaa
    17821 gttatccggc atgatgaggt tgtgacggac cgcatacgca ccaatacgca gcgcaagcgg
    17881 aagatcgccg cagtcaatcg cccacaccat cagcgtggca atcacttcgt cctgttgccc
    17941 gccgtcagcc tccagcgttc cctcaatcca gccggaaaag tccggcaaca acgctttttt
    18001 gatgtcggct ttcgcgcttc tggcctgtac gcccttaagc cgggcctgtg ccagacgcag
    18061 acgatacagc acctcttcat gcgcggtacg cgcggcgtgg tccacacctt cattcgcccg
    18121 gcctgcgcgc tgtgccatca cgttctgcca gtgttgctgt gcaggagtaa tcattttttc
    18181 tctccgttac aggcgggcat gatgcccgcc gtgagttgat tagctgtcgg cgaacttcag
    18241 gccagtgacc atcgcgcact tgccatagtc ttcaacgaca taagcgtcat tgatggactg
    18301 gtaggtggcg atgcggttgt attccggttc gtctttcatc agacgacgca t
//
LOCUS       DAZRUF010000067        18304 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZRUF010000067 DAZRUF010000000
VERSION     DAZRUF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.067"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..576
                     /locus_tag="ACHXOL_001838"
                     /pseudo
     CDS             1..576
                     /locus_tag="ACHXOL_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            679..1181
                     /locus_tag="ACHXOL_001839"
                     /pseudo
     CDS             679..1181
                     /locus_tag="ACHXOL_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1248..2705)
                     /gene="nuoN"
                     /locus_tag="ACHXOL_001840"
     CDS             complement(1248..2705)
                     /gene="nuoN"
                     /locus_tag="ACHXOL_001840"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR6808835.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2712..4241)
                     /gene="nuoM"
                     /locus_tag="ACHXOL_001841"
     CDS             complement(2712..4241)
                     /gene="nuoM"
                     /locus_tag="ACHXOL_001841"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR6808836.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4405..6246)
                     /gene="nuoL"
                     /locus_tag="ACHXOL_001842"
     CDS             complement(4405..6246)
                     /gene="nuoL"
                     /locus_tag="ACHXOL_001842"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR6808837.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6243..6545)
                     /gene="nuoK"
                     /locus_tag="ACHXOL_001843"
     CDS             complement(6243..6545)
                     /gene="nuoK"
                     /locus_tag="ACHXOL_001843"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR6808838.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6542..7096)
                     /gene="nuoJ"
                     /locus_tag="ACHXOL_001844"
     CDS             complement(6542..7096)
                     /gene="nuoJ"
                     /locus_tag="ACHXOL_001844"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR6808839.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7108..7650)
                     /gene="nuoI"
                     /locus_tag="ACHXOL_001845"
     CDS             complement(7108..7650)
                     /gene="nuoI"
                     /locus_tag="ACHXOL_001845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR6808840.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7665..8642)
                     /gene="nuoH"
                     /locus_tag="ACHXOL_001846"
     CDS             complement(7665..8642)
                     /gene="nuoH"
                     /locus_tag="ACHXOL_001846"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR6808841.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8639..11365)
                     /gene="nuoG"
                     /locus_tag="ACHXOL_001847"
     CDS             complement(8639..11365)
                     /gene="nuoG"
                     /locus_tag="ACHXOL_001847"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR6808842.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11418..12755)
                     /gene="nuoF"
                     /locus_tag="ACHXOL_001848"
     CDS             complement(11418..12755)
                     /gene="nuoF"
                     /locus_tag="ACHXOL_001848"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR6808843.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12752..13252)
                     /gene="nuoE"
                     /locus_tag="ACHXOL_001849"
     CDS             complement(12752..13252)
                     /gene="nuoE"
                     /locus_tag="ACHXOL_001849"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR6808844.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13255..15057)
                     /gene="nuoC"
                     /locus_tag="ACHXOL_001850"
     CDS             complement(13255..15057)
                     /gene="nuoC"
                     /locus_tag="ACHXOL_001850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR6808845.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15151..15813)
                     /gene="nuoB"
                     /locus_tag="ACHXOL_001851"
     CDS             complement(15151..15813)
                     /gene="nuoB"
                     /locus_tag="ACHXOL_001851"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR6808846.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15829..16272)
                     /gene="nuoA"
                     /locus_tag="ACHXOL_001852"
     CDS             complement(15829..16272)
                     /gene="nuoA"
                     /locus_tag="ACHXOL_001852"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR6808847.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16901..17839)
                     /gene="lrhA"
                     /locus_tag="ACHXOL_001853"
     CDS             complement(16901..17839)
                     /gene="lrhA"
                     /locus_tag="ACHXOL_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR6808848.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4566 a         5089 c         4719 g         3930 t
ORIGIN      
        1 tttaccaacc ctgggtcact gcattatcgc caacgccccg acacgtcagt cctgagcaat
       61 tagaaaaaac agttcgttat cttacacaaa ctgtgcatcc acgtaatgcc gacaatattg
      121 ataatctgaa taggtccgta gagtatataa aagagttctt tgtcagtagc ggtgccagag
      181 ttaccgcgca gagcgtcccc gattggctat tacgactctg cgcctggtag ccaggattac
      241 ccttacccgc aatgtcctgg ctttatcccg atcgggggga ctttattgcc tggtcggcag
      301 aatacaggat atcaacgccg ttcgtcaggt aaaaacggca ttgttatcat cccgggattt
      361 atctgtttat tctatgaatg ccccatggtt tattcccggt attgatttct ctgaccacct
      421 gaattattgg caacacgata ttcccgccgt aatgattact gacaccgctt tttatcgtaa
      481 taaacaatac cacttgcccg gtgatacagc agacagattg aattatcaga aaatggctca
      541 gatggtgtta taaccttgtt acacaacagt aaataataaa aatttctcga ggcgggtcca
      601 gaaacagcga catgtacatt ttattttccc tcgtctgttt taagatgcga aacataaaat
      661 tcaatggagg aaaaataaat gggaatgatt ggttattttg cagaaattga ctcagaaaag
      721 attaaccaac tgcttgagtc tactgaaaaa cctttgatgg ataatataca tgacacgctt
      781 tcaggattac gccgactgga tattgataaa cgctgggatt ttttacactt tgggctgaca
      841 ggcacccctg cttttgatcc cgcgaagaat gatccgctga gtcgtgccgt gcttggtgaa
      901 cacagcctcg aagatggcat agatggcttc ctgggattga cctggaatca ggagttagcc
      961 gcgacaatcg accgactgga atcactggat cgaagtgagc tgcgtaaaca gttttccatt
     1021 aagcgactca atgaaatgga gatttatccg ggagtgacct tttcagaaga gttagagggg
     1081 caactctttg cttcgatcat gctgatatgg aaaagctgat tagcgcgtat cgacgtatgc
     1141 tgcgacaagg gaatcatgcg ctgacggtta ttgttgggtg atggtgtcag ggcaccaaaa
     1201 aaaaccgccg aacatgtcgg cggctttctg acttacaaac tgatagctta catcagcggc
     1261 attgctaacc gcacaatgct aatcagcggt tgtggccata cacccagcac cagtaccaac
     1321 agtgcagaga tcagcaccac gataccgcct gcgctgtact gccagtttga cggtgcatcg
     1381 cgacccggtt gttccggggc gtgcaggtac aggctcaccg ccacgcgcag gtagtagtaa
     1441 aggccgcttg ccgaaccgac aaccacggcg cccaccagcc accacaagtg tgcctggaca
     1501 ccgaccgcca gcacgtagaa cttaccaata aagcccagcg tcatcggaat accggccaga
     1561 gacagcatca tcaccgtcat caccgccgcg aggatcgggc gatgccagaa cagtccgcgg
     1621 taagagtaca gggaatcagc atccgggcca cgatacgggc tggacatcag gctgaccacg
     1681 ccgaacgcgc cgaggctgct aaacagataa ccggccaggt aaacccctac cgcttccatc
     1741 gacatctcgc cagtttgcag cgcaatcagc gccaccagca gatagccgag gtgagagata
     1801 gatgagtaac cgagcagacg tttgatattg gtctggctca gcgccatcag gttaccgaag
     1861 atgatggagg caaaggcgat aatcgccagc accacgcgaa tcgcttcgct gtcacccatc
     1921 ggtgcgtaga ggaacagacg catcaccaca ccgaagatag cgattttgct cgccgtcgcc
     1981 aggaaagtgg aaaccggcgc aggcgcgccc tggtatacgt ctggcgtcca caggtggaac
     2041 ggcaccagag agagtttgaa gccgaggcca acaatcatca ggccgaaacc tgccagcaac
     2101 agcggctcgt tgagcatacc gtcgccaagg tttttaccca acgcgacaaa cgacaggtcg
     2161 ccagactgcg catacaccag cgccatacca aacagcagga aagaagacgc tgcggcagaa
     2221 aggatggtgt atttgatact ggcttccagt gaacgtttct ggcggaaagc gtaaccgacc
     2281 aggccaaaca gcggcaaaga gatcagttcg ataccgagga acagagacgc cagatggttg
     2341 gcattcgcca gcaggatccc gcccagcgcg gcaattaaca ccaacaggta gaactcgtcc
     2401 ttgttgtcgt tatagccttc aagccacggg taggcgaaag tacaggtggc gaggctcgcc
     2461 aacaatacca gcccggtgta aagcatggcg aaaccatcaa cgcgcatcag cggcgtaacg
     2521 tccatagcgc ctgcctggcc gacaaaccag agcgaaacca gcgccgcgtt aagcccaata
     2581 accgagagcg tagcgttgag gaaatgattg cgtcgccacg caatggagag catcacaacc
     2641 accaccgtca agccgacgat cagcaacggt agcagtgcga tcaggttttg tggagttatt
     2701 gtcatggcga tttacggcct tgtagtagta acggaattaa caaaccactg ctggatattg
     2761 ccaatcgcgg agtgcgaggt atccagaatc ggctgcggat agaagccctg cagtaccagc
     2821 agcaccacca gcaacaggat cataaacagc tcacgcagcg acatccctgg cagttcctgg
     2881 ctggcaatct cgcttttcgc tttaccgaag taagcgcgat gcaacatcgc cagcgaataa
     2941 acagatgcaa agaccagccc aaaggtagag ataacggtaa tcaccgggac aacctggaag
     3001 ctgccgaaca gaatcataaa ttcgccgacg aagttaccgg tgccaggcat cccaagcgtt
     3061 gccaccgcaa agaacagcga cagtgctggc agccatttca tcttgctcca cagaccgccc
     3121 atcatgcgca tgtcgcgggt atggatacgt tcataaagct gaccacaaag aataaacaga
     3181 cccgccgccg acaagccgtg cgcaatcatc tggattaccg cgccctggta ggccaactgg
     3241 ctgccggtgt agatagcaat cagcacgaag cccatgtggg aaaccgaggt gtaggcgatc
     3301 agacgtttga tatcggtctg ggcgaaggcc atccacgcac cgtagaagat gccgataaca
     3361 cccagccaca tagcaattgg cgcgaactct gccgacgcgt tcgggaacag cggcagggag
     3421 aaacgcagca aaccgtaagc ggcagttttc agcaagatcc ccgcgaggtc aacggaaccg
     3481 gcggtcggag cctgggagtg cgcatccggc agccagccat gcagcggaac caccggcatt
     3541 ttgactgcga aggcgatgaa gaagcccagc attaacaggt attccgcacc actggacatt
     3601 ggcgtattca gcagctcttc atagttgaag gtccagacgc cggtcgcatt gtagtgaaca
     3661 aaaaccagcg ccaggatggc aatcaacatc accagaccac tcgcctgggt gtaaatgaag
     3721 aacttggttg ccgccgtgat acgcgtttta ccgtcagagg ctttatgccc ccacagtgcg
     3781 atcaggaagt acatcggcac cagcatcatt tcccagaaga agaagaacag gaacatgtcg
     3841 atggcaagga acacgccgat aacgccgccc aggatccaca tcaggttgag gtggaagaag
     3901 ccctgatatt tttcgatctc tttccacgaa catagtaccg ccagcacacc gagcagaccg
     3961 gtcagcacga ccatcagcag cgacagcccg tcaatggcga gatgaataga gatacccaaa
     4021 cgcgggatcc acggcatgtc gaattcagac tgccactgcg gaattccggc ggattgcgtc
     4081 agtgaataac cgccctgcaa ccacagttgc agcgacagcg ccagcgtcaa tcccatggtg
     4141 atcagcgcga tccagcgtgg caccttgacg ccaaagcgtt cggtctgcca gcacaggaag
     4201 ccgccaataa agggaattaa tattagccag ggtagtaaca tggcgatctt tattccttgt
     4261 aaaagtcccg tcaggaccgg attttcaacg aattctcacg acagaaaatt ctcttcggac
     4321 tgggggtagt gtcggatgca ccgcgaaggc ggcttatccg acctacgttc ttatatgccc
     4381 aggggcaatc ccacaatcct taactcaacg cagtaccatc aacagtgcca gcaccacgac
     4441 cgcaccgatg ctcatggatg ccacatacca gcgcagatag ccgttctcac ttaacagcag
     4501 acctttacct gcaaagcggg aaaggacagc cggaatgttc atcattgagt tcagcggatc
     4561 gcgtttcagc aaccaggcaa tacccaggaa cggcctgacg aacactttgt cgtacagcca
     4621 gtcaaagccc caggcgttgt accaccaggt actcagcagg cggcccggcg cactgttggc
     4681 gatggaggtc accagagtac gtttacccag ccacagccaa gctgccagca gaatgccgac
     4741 aatcgcgacc acgccagagg taatttccag ggtcaacatg ctgccgtgcg ccagttccgt
     4801 cgtttgcgga agcacgccct gcagcggcgg tacaatcagt gcgccaacga aggtggaaag
     4861 gatcagcagc acaatcagcg gcaggctgtg agttacccct ttcacggcgt gagcgtgaat
     4921 ttgttctttt ccgtggaaga cgatgaaaat catacggaag gtgtagagcg aggtcataaa
     4981 cgcaccgacc agacctgcca ccatcagatt gatatgacca ttcgccatcg cacccgcgag
     5041 gatctcatcc ttactgaaga agcccgcagt gaccagcggc agtgccgaca gtgctgcgcc
     5101 gcccaccagg aagcagagat aaaccagcgg aatagattta cgcagaccgc ccatcttgaa
     5161 gatgttctgt tcgtgatggc aggccagaat gacggaaccg gatgccagga acagcagcgc
     5221 tttaaagaac gcgtgagtca tcaagtggaa aatcgccgca tcccatgcct gcacgccaag
     5281 cgcgaggaac atgtagccaa tctggctcat ggtagagtaa gcgagaacac gtttgatgtc
     5341 ggtctgtacc agcgcggcaa aaccggccag cagcagcgta accgccccga caatgcccac
     5401 cagatgcaga atttccggcg tcatcaggaa caggccgtgg gtacgggcga tcaggtagac
     5461 acccgcggtt accatggttg cggcgtggat cagcgcggag acaggcgtcg ggcccgccat
     5521 cgcgtcggca agccatgtct gcaacggcaa ctgcgcagat ttaccgaccg caccgcccag
     5581 cagcatcagc gtcgcccaca tcagcatgtt attgccgtca gcaaagtgcg ctggtgccag
     5641 ttccaccatt tcgcggaagt tcagggtgcc cagttcgttg taaagaatga acagtgcgaa
     5701 agcgaggaaa acgtcaccca cacgggtcac gacgaacgct ttcattgccg ctgcgccatt
     5761 cttcggatcg gtgtaataga aaccgatcag cagataggag cacaggccca cgccttccca
     5821 gccgaggtac atcagcagca ggttgtcggc aagcaccaga actaccatgc tggcgatgaa
     5881 caggttggtg taggcgaaga agcgagagta gccctcttca ccgcgcatgt accaggaggc
     5941 gtacatgtga ataaggaaac ccacaccagt gaccaccgag agcatggtca gcgacaggcc
     6001 gtccagcacc aggttaaaac cgatgttaaa gtcgcctacc gacatccacg tccacagcgg
     6061 ctggctgtat gcctgctcgc cgttagcgaa gaaatcaacg ccgataaagg cggttaccag
     6121 cgccgccagg cccacagagc caacgccgac gatcgccgaa acgttttcag accagcgccc
     6181 acgggagaac gccagcagga cgaagccaat caatggcaaa ataatggtta aggcaagcat
     6241 gttcatccgc gcatctcact tactgaatcg atgttcaggt tctggcgacg acggtgaagt
     6301 tgcagcagca gcgcaaggcc gatactcgct tctgccgccg cgaggctgat ggcgagaatg
     6361 tacatcacct gaccgtcggt ctgaccccag tagcttccgg cgacaacaaa ggccagcgcg
     6421 gaggcgttaa tcatgatttc cagaccaatc agcataaaca gcagattgcg acggataacc
     6481 agaccggtta agccaagaat gaataagatt gccgcgagga tcagtccatg ttgtaagggg
     6541 atcatgcgtg ctcctccgtt tttcttttcg cgctgtcgtc tttacgattg ctcagcactt
     6601 cacccgcacg ctcttcacga ccgacgtgga aggccacaac cagacctgcg agcagcagca
     6661 tagaagccag ttccaccgcc agtacgtaag gcccgaacag cgtaataccc actgctttag
     6721 cactgattgg cgtaccgtcg ataccctgat cgttaacacc gaggatggcg taaacaatca
     6781 ccaccagcat gatggccgac aaaattgccg gaccaatcca cacctgcggt ttcagccact
     6841 ggcgttcctg ttcgatttct gaaccgccca ggttgagcat catcaccacg aacacgaaca
     6901 gcaccataat ggcacccgcg tagacgataa tttccagcgc accggcgaag taagcgccca
     6961 gtgagaagaa caccccggaa atcgccagca gcgaaataat caggtacagc agtgcgtgta
     7021 ccggattggt atgggtgatc actcgcaagg tcgcaagtat ggctatcagg ccacagatat
     7081 aaaaagcgaa ctccattgcc cctctcctta cggtaacagg ctcttgacgt cgataggctt
     7141 ggcttcgttc tctgcttcgc ccttatcttt gccgtcgatt gccatacctg ccatccggta
     7201 gaagttatat tccgggtatt tgcccggacc ggagatcagc agatcctctt tttcgtaaac
     7261 cagatcctgg cgcttgtatt cccccatttc gaaatccggg gttaactgaa tcgccgtggt
     7321 cggacaggct tcttcgcaca gaccacagaa aatgcagcgt gagaagttga tgcggaaaaa
     7381 ttccggatac cagcgaccgt ctttggtttc tgctttttgc agcgagatac agccgaccgg
     7441 gcaggctacc gcgcagaggt tacaggctac gcaacgctct tcgccgtccg ggtcgcgggt
     7501 cagaacgata cgaccacgat aacggggcgg cagatagatc ggctcttccg ggtacattcg
     7561 cgtttcgcgt ttggcgaacg cgtgcaggcc gatcatccag atactacgaa cctgggtgcc
     7621 gaaacctact aacaattctt ttaaggtcat ggtcttattg ccccttattg cgcctgccag
     7681 agaatgacag ccgccgttac cagcaagttg atcagcgtca gcggcaggca gattttccag
     7741 ccgaaggaca ttacctggtc ataacgcgga cgcggtaacg acgcacgaat caaaatgaac
     7801 atcatcataa agaacgcggt tttcagcgcg aaccagatga atggcggtaa caacgggcct
     7861 tgccagccac cgaagaacag cgtcaccatc aatgcagaga tggtcacaat cccgatgtac
     7921 tcacccacga agaacagacc gaacttcata ccggaatatt caatgtggta accatccgcc
     7981 agttcctgct cggcttccgg ctggtcaaac gggtgacggt gacataccgc cacgcccgcg
     8041 atggcaaagg taataaaacc aaagaattgc gggataacgt tccacacatg cgcctggctg
     8101 ttgacgatgt cggtcatgtt gaatgaaccg gcctgcgcca ccacgcccat cagggaaagc
     8161 ccgaggaaca cttcgtagct cagggtctgc gcagaagcac gcatcgcacc cagcaacgag
     8221 tatttgttgt tacttgacca gcccgcaaac agcaccgcgt aaaccgccag acctgccatc
     8281 atcaggaaga acaaaatccc gatgttcagg tcggcaacca cccaacccgg actgactggc
     8341 acaatcgcaa aggccagcag cagcgaggta aaggcaatca tcggtgccag ggtaaagatg
     8401 acgcgatccg agaatttcgg gatccagtct tctttaaaga acattttgat catgtccgca
     8461 accagctgga gcgaaccgcc ccagccaaca cggttaggtc cgtaacggtt ctggaacaga
     8521 cccagcaggc gacgttcgcc aaagctcatg aatgccccgc aggtgacaac caccagcagg
     8581 atcaccaccg ctttgaggat ggtcagcagg atctcaatca gttccggtga tatccaactc
     8641 attgttgtgc ctccttgaga tcctcaagat gcgcgccagc cagcaccgga gcaataccgg
     8701 acatacccat cggcaagccc acctgccctg ccgtcagccc ttcagcgatt tcaaccggca
     8761 gcgtgaccgt attgccatcg taactaaagg agacgtgtgt acctgcgttc acacctaact
     8821 tcgcggcatc cgctgggttg agtttgatgt acggctgcgg catacggctc tggaagaccg
     8881 gagcacgctg tgacaattca tcgctgccaa acaggtggta atacggcgcg atacgccatt
     8941 tcccgtcctg cggctggaag cgtgccggta cgctggtgaa gtaatccaga ccattttcgc
     9001 tggtttcaaa cagacgcacg cccggatcgc caaagcgcag tttgccgccc acttcgtcct
     9061 ggaatttgtt ccacgcctgc ggggagttcc agcccggcgc ccaggcaaac ggcacttgcg
     9121 aacggtgcgc agtcggctgg ttgttacctt ccatcgagaa ggtgaacatg gtgtcaatat
     9181 cctgcggctg acgcggctca tgaacgctga tattggcgcg catggcggta cgaccgctgt
     9241 aacggtgcgg ttcacgggcc agtttctgcc cacgaatacg gaatgtcgca tccggcgcag
     9301 catctttgat acctgccagt tccgggattt tcgccacaac agcgtcaatc acatggtcga
     9361 gctgcgtcca gtccacttca cggctcagca gggtgctatg cagcgagtgt aaccagcgcc
     9421 agctttccag catgacagtt ttgctgtcgt aataagcagg atcgtaaacc tggaagaaac
     9481 gttgggcgcg gccttcgttg ttgatcaccg taccgtcgct ttcagcaaaa ctggcagcag
     9541 aaagtaccag atgggcgttt tccataatcg ctgtgcgttg atgatcaacc accatcacca
     9601 gcggcgcttt agccagcgca gcattcacgc gggtagcgga agcgtgacga tgcagatcgt
     9661 tttccagcac caccaccgcg tcggcgcgtc cggtttccag ttcggtcaac gcttcttcaa
     9721 gcgaaccgcc acccataatg cccagcccca tgctgttgac ggaacgggca atcatggtga
     9781 taccaacgtc agcgccgcga cctttcaggg ctttcgcgac gttagccgca gcctgaatca
     9841 cctctatgct accggcgttc gtcccggaga taatcaacgg tttcttcgca cctgccagtg
     9901 cctgcacgat gacgtcgatt ttgctttgca gctcaggttc gataccgtca accgctggcg
     9961 cagagttatc cagcgcatgg gcgatggcaa aacctaaacg cgcctgatct tcaaccggtg
    10021 cgcggtaagt ccacgccgcg atatcatcca gacgggtgtc atcaacgttg gtaacaaaca
    10081 gcggatgctt cgcacgttga ccgatgttga ggattgccgc aatctgccag tcagccactt
    10141 tctgtgctgc cgccatttcg cgcgctttac ctttcacagc ctgacgcact gccagcgcga
    10201 cgcgcgcgcc ggtctgggta acgtcttcgc ccagcaccag taccgcatcg taagattcga
    10261 tttcgcgcag agccggagta taaatgccgc cttcacgcag cactttcagc gccagttgca
    10321 gacgttcctg ctcaccgtga gcgataccgg tgtagaagtt ttcttcgccc accagttcac
    10381 gcagcgcaaa gttgctttcc acgctggcac gcggagaacc aataccgatc actttcttcg
    10441 actgacgcag aatatctgcc gcgccctgca ttgcctgttc ggcgttgagg gtaatgaaat
    10501 catcgccacg acgctgtact ggctgacgcg gacgatcctt caggttgacg taaccgtaac
    10561 cgaaacgacc acggtcgcag aggaagtagt ggtttaccgt accgttgtaa cggttttcga
    10621 tacgacgcag ttcgccgtaa cgttcaccgg ggctgatgtt acagccgatg gaacattgct
    10681 ggcagatgct cggcgcaaac tgcatatccc atttacggtt gtaacgctcg gagtgcgttt
    10741 tgtcggtaaa tacgccggtc gggcaaattt cgaccaggtt accggaaaat tcgctttcca
    10801 gcgtgccgtc ttccgggcga ccgaagtaga cgttgtcgtg cgcaccgtaa acgcccagat
    10861 ctgtaccgtc agcgtaatct ttgtagtaac gcacacagcg gtagcaggcg atgcagcggt
    10921 tcatttcgtg agagatgaat ggccccaaat cctgattacg gtgggtacgt ttggtgaaac
    10981 ggtagcgacg gaagctgtgt ccggtcatca cagtcatatc ctgaagatgg cagttaccgc
    11041 cttcttcaca taccggacag tcgtgcgggt ggttggtcat taaccactcg accacacttt
    11101 cacggaactg tttcgcttct tcgtcgtcaa tggaaataaa ggtgccatcg gaagccggtg
    11161 tcatacagga catcaccagg cgaccacgcg tgtcttccgc gttttggtat tgcttcaccg
    11221 cacactggcg gcaagcaccg acgcttccca gcgccggatg ccagcaaaag taaggaatat
    11281 caaggcccag agacagacaa gcttccagca ggttgtccgc tccgttgacc tcgtattctt
    11341 tgccgtctac atgaattgta gccattagca tgcttccagt tttctcggtc agggaccgag
    11401 cgttaatcga aattcggtta ccagcgctct ttcagcaggt tcggctgaat cccattaatc
    11461 aaatgggtat tgctgaacgg ctgtttgatt cccgcctcaa attcttcgcg gaaatatttg
    11521 atggcgctct gtaacggctc cactgcacca ggtgcgtggg cacagaaagt tttacccggg
    11581 cctaagaatc gacacagttg ctcaagtgtt tcgatatcgc ccggctggcc ttcgccacgc
    11641 tccagcgcac gcagaatttt cacgctccac ggcagaccgt cgcggcacgg cgtacaccag
    11701 ccgcaggact cacgggcgaa aaactcttcc aggttacgca ccagcgacac catgttgatc
    11761 tcatggtcaa ccgccatcgc cagcgccgta cccagacggc tgcccgcttt accgatactt
    11821 tcgaattcca tcggcagatc aaggtgcgct tcggtcagga agtcagtccc cgcgccgcct
    11881 ggctgccagg ctttaaattt cagaccatca cgcataccac cggcgtaatc ttcgaggatc
    11941 tcgcgtgcgg tggtgccgaa cggcagttcc cacagtcccg gatttttcac ccgaccggag
    12001 aagcccatca gcttggtgcc agcatcttta cttttcgaga tgttctgata ccactccacg
    12061 ccgttagcga ggatcgccgg aacgttacac agggtttcga cgttgttgac acaggtcggt
    12121 ttgccccata cgccggaggt tgccgggaag gggggtttcg agcgtgggtt agcacgacgc
    12181 ccttccaggg agttgattaa cgctgtttct tccccgcaga tgtagcgccc tgccccggta
    12241 tggacgaaca gttcgaagtc gaaacctgtt cccataatgt ttttgccaag caagcccgct
    12301 tcagtggctt cggcaatggc gcggcgcaga tttactgccg cttcgatata ttcgccacgc
    12361 aggaagatgt agccacggta agctttcagc gcaaacgcgg agatgagcat accttccacc
    12421 agcaggtgcg gcagttgctc catcaacagg cggtctttat aggtgcccgg ctccatttca
    12481 tcggcgttac acagcaggta acggatgttc atggattcgt ctttcggcat caggctccac
    12541 ttcaggccag tcgaaaagcc cgctccgccg cgccctttca gaccagcgtc ttttacctga
    12601 ttaacgattt cgtccggaga cagcccggtc agcgccttac gcgccccttc gtaaccgttt
    12661 ttgctgcggt attcgtccag ccacactggc tgtttgtcat cgcgcagacg ccaggtcagc
    12721 ggatgcgttt cgggagtacg gataatgttt ttcatttata ccgctccagc agttcaggga
    12781 tcgcttccgg ggtcagatgc gcgtgagtgt cctcatcgat catcatgttt ggccctttat
    12841 cacagttccc caggcagcaa gttggcagca gcgtaaagcg gccatcaaat gtcgtttgcc
    12901 ctggtttgat gttcagcttt ttctcgagcg ccgcctgaat accctgatac ccgttgatat
    12961 gacagaccac gctgtcgcaa taacggatca catggcgacc aaccggctgg cggaagatct
    13021 gactgtagaa cgttgccaca ccttcgacgt cgcttgccgg aatacccagc acatcggcga
    13081 tcgcgtggat cgcaccatcc ggcacccagc cacgctgctt ctgaacgatt ttcagcgctt
    13141 caatggacgc cgcacgcggg tcttcgtagt ggtgcatctc gtgctcaatc gcttcacgct
    13201 ctgccgcact cagctcaaaa gcctcggttt gtggttgttg attctcgtgc ataattagcg
    13261 gtccacatct gacataacaa aatcgatact gcccagataa acaatcaggt cagacaccag
    13321 gctgccgcgg atcgccgccg gaatttgctg caaatgcgca taactcggcg tgcggatacg
    13381 agtgcgataa ctcatggtgc taccgtcgct ggtcaggtag taactgttga tccccttggt
    13441 cgcctcaatc atctggaaag attcattggc aggcatcacc ggcccccacg acacttgcag
    13501 gaagtgggtg atcagggttt cgatatgttg cagcgtgcgc tctttcggcg gcggcgtggt
    13561 cagcgggtga tccgctttga acgggccttc cggcatgttg ttgaggcact gctcaagaat
    13621 gcgcagactc tggcgcagct cttccacttt cagcattacg cgggtgtagc agtcagaaac
    13681 accaccacca accgggattt caaagtcgaa gttttcatag ccagaataag gacgcgcctt
    13741 acgcacgtcg aagtcgatcc cggtagcacg caggcccgcg ccagtggtgc cccactccag
    13801 cgcctctttc gcgccatagg cggcaacgcc ctgggaacga cctttcagaa tggtattttg
    13861 cagcgccgct ttctcgtaag acgccagacg tttcggcatc cagtcgagga actcacgcag
    13921 caggcgatcc cagccgcgcg gcaggtcgtg cgctacgccg ccaatacgga accacgccgg
    13981 gtgcatacgg aaacccgtga tcgcttccac cagatcgtaa attttctgac gatcggtaaa
    14041 ggcgaagaac acgggcgtca ttgcgccgac gtcctgaata aaggtcgaga tgtacagcag
    14101 gtggctgttg atacggaaca gttcggagag cataacgcga atgacgttaa cgcgatccgg
    14161 cacggtgatc ccggccagtt tctctaccgc cagcacgtaa ggcatttcgt taacgcagcc
    14221 gccgaggtat tcaatacggt cggtatacgg aatgtagctg tgccaggact ggcgctcgcc
    14281 cattttctcc gcaccacggt ggtggtagcc gatgtctggt acgcagtcga caatctcttc
    14341 gccgtcgagt tgtaaaacga tacggaaagc cccgtgcgcc gacgggtggt tcggaccgag
    14401 gttgaggaac atgaagtcct cgttttcggt gccgcgcttc atcccccact cttccggttt
    14461 gaaggtcagg gcttccatct ccagatcctg tttggctttg gtcagctcaa acggcgagaa
    14521 ttcggtagcg cgcgccgggt aatctttacg cagcgggtga cctttccagg tttgcggcat
    14581 catgatgcgg cgcaggttcg ggtgaccgtc gaaagtaatg ccaaacagat cccaggtttc
    14641 acgctcatac cagttagcgt tcgggaacag tttggtgaag gtcggtacgt gcaggtcgtt
    14701 ttctgccagc gccaccttca gcatgatgtc gcggttacga tcgatagaaa tcagatggta
    14761 gaaaacggaa aaatccgcgg caggtaaccc ttcgcggtgt gtgcgcagac gttcgtccat
    14821 gccgtgtaag tcaaacagca tgacgtaagg tttcggcagt ttctttaaga aatcgccaac
    14881 ttccagtaat tgttcacgct tgatccacac aacgggaacc ccggtgcgag tcgcctgaac
    14941 agtaaaggca tccggcccaa aacggttgcg cagttcgcca atcaccggat catcaagatg
    15001 atcacgggtc tgccaggcgg gttcttgcgc ggttaagtcg gtcatattgt tcaccattgc
    15061 aaatggtccg tggtgactgt cggcgcagta agcttcgcga aatagattta ggaataagcg
    15121 aagaaaaatc cctttgccga caggcgcaaa ttaaatctcg tcaggtgtac gcaggttggt
    15181 tacggcaatg cgttcaccgc gcttgcgttc gcgctctgat tgcatattgg cgcgataaac
    15241 gccctgatcg ccaaccaccc aggagagcgg acgacgttct ttgccgatag attcctgcaa
    15301 cagcatcagt gcctgcatgt acgcttcagg acgcggcggg cagcccggga tatacacatc
    15361 aaccgggatg aatttatcga cgccctgcac aacggaataa atatcgtaca taccaccaga
    15421 gttggcacag gcacccattg agataaccca ttttggttcc agcatctggt catacagacg
    15481 ctgaataacc ggtgccattt tggtaaagca ggttcctgca accaccatca ggtcagcctg
    15541 acgcggcgaa gcacgcaata cttctgcgcc aaaacgcgcc acgtcatgca ccgcggtaaa
    15601 cgaagtcacc atctcaacgt aacagcagga aagaccgaag ttatacggcc aaattgagtt
    15661 tttacgaccc cagttaacca tgtcattgag cttgcccata aacacgtttt tgttaacttc
    15721 ttgctccaga gggtcggtta cgatctcctg cttttgcagg gggtaacggt cgttctcacc
    15781 gttgggatct atgcgggtga gcgtataatc catcttaatg cctcgcggtt agcgttgacg
    15841 attagcgata ctgttcgttt ccgggttcat acgctcgcgg cgtgaacgcg cgggcgtcca
    15901 gtccagcgcg ccaatacgca ccagataaac cagacctgcc agtaacacaa aaataaaaat
    15961 tgcagcttcc acaaagccta cccagccgct ttcgcggata gaggttgacc atgcgaacag
    16021 atacagcgct tcaacgtcga agataacgaa gaacatggcc accagataaa acttggcgga
    16081 caggcgtaag cgggcggagc cgaccgagtc gataccggat tcaaacggca cgtttttcga
    16141 cctcgcgcgt gcgcgaccgc ctaaaaacca accgcctacc agcatcaggc aacacaggcc
    16201 aatggcaacg ataagaaaga tagcgaatgc ccagtgatga gcgatgactt cagtggatgt
    16261 tgacatactc attgcttact catcaaaagt agcgccagat tcactgctct tcacggcaga
    16321 tggacgccac atcgattcat ggggaggaat aaaaaaaacc ttacaatcac tgtagaaatt
    16381 cttttataca gctaattgat gtggtctttt actcctttct ataacctttt gtcaacttta
    16441 acaaaagttt cttcacatta gtttacataa tatcaacacc attagcattt aatgcccttt
    16501 cacgccagat ccttgacgac tccaggataa ttagatgttg ttgaatcgtg tccgttgtga
    16561 agcaatggaa aaaatacggg tctattttga caggaatttg tgtcgattcc tccccccaaa
    16621 agagagtatt ttcttgatct gtgacacgct tttgtcattc cataacaaaa acgcagcaac
    16681 aaatttacgt atttttaaca tcattgtagc aggtgatttt tttcaggcga ttatttgtgc
    16741 gttcgggacg tgaatctctg gtgattgaaa aatgaacagt tttgtacgtt ctgcactatg
    16801 cgaggaagga tttttactaa aaaaaagccg ctggggttta aaacaccccc agcggctcgt
    16861 ttttacacta ttgtctcagg aattatctat cgtccgtcga ttactcgata tccctttcaa
    16921 tcaacaatga atcatcccct tccggagcag acattgggct gtattgccac ggattatggt
    16981 agctctccat cgcctgatag atcacctggg ccagctcatt attactggac gggtcatagc
    17041 acagcaaata ttcggtatca ggtagcggcg gtaagccatc tactccactc agcacacgca
    17101 ggtccgggct catcatttca atcggccttg ccgtcacgcc aagaccggct ttcactgccg
    17161 cacgaacggc cggaagcgtc gaggcgacat aagccagtcg ccatggaata tctgctttat
    17221 taagcgtcgc cagcaccata tcgcgaaacg gacttggatc atccagcaat acaagaggga
    17281 tcggctcgcc tttttgcaat acgtattccg ctgcgcagta ccagtgtgtt ggcgaggtac
    17341 gcaggttcaa tgccttaaac gctgaaggac gatgggtggt taccatcaaa tccacttcct
    17401 gggattccag catttctgcc atataggcat tacgtttaac gcggacatcc agcgctaatt
    17461 tcggataaac cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg gcagattcat
    17521 ctgaagcacc gatagttaac acgccctgaa gattactgaa cattaatgat gagcaggcct
    17581 catcattaaa acgcagtatt ttcctggcgt aaccaagaag ttgaatgcca tgttcagtta
    17641 acagtttgtt gcgaccgtga cgagcgaaca gttctttccc aacgagttgt tccagacgct
    17701 gcatttgctg acttacggcg gactgagtac gacacacagc ggcagctgcg gcagcaaaag
    17761 tgttcagatc ggcaacagca acaaatgttc tcagcagatc gaggtcgagg ttaattatcg
    17821 gacgatttgc acttatcata tattatcact tactggcggc tcatactgag ctggttaatg
    17881 ctgtgcacac acaaacaagc aattccattt gtaatgtgcc tccctggcag tttcatccgg
    17941 aacccggacg aaagtaaaaa tgcatatgag ttgcactaaa aaagcgactc acattgttcc
    18001 gttataatgc ctgaagtaga tcacagaata tatcttcagg gatcgcatat ctattaagtt
    18061 actcactctt ttctatttat gacatgcgcg tgtttgtata aatgtaaatg tgagtccttg
    18121 ttccactctc gtgcagcatc gctggtcata cgcgaacacg tacaaacagc aatggtgagg
    18181 cggcatcaag agcagggatc cgtcatttat gcgagcattt taccccaaaa accttttatt
    18241 tataagggtc attgcgaatt atctgatgca aagttatgtt atgttaggca aagtaatctt
    18301 ctat
//
LOCUS       DAZRUF010000068        18137 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZRUF010000068 DAZRUF010000000
VERSION     DAZRUF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18137
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.068"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            31..738
                     /locus_tag="ACHXOL_001854"
     CDS             31..738
                     /locus_tag="ACHXOL_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR6808849.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            735..2186
                     /gene="djlC"
                     /locus_tag="ACHXOL_001855"
     CDS             735..2186
                     /gene="djlC"
                     /locus_tag="ACHXOL_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR6808850.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(2246..3916)
                     /gene="hscC"
                     /locus_tag="ACHXOL_001856"
     CDS             complement(2246..3916)
                     /gene="hscC"
                     /locus_tag="ACHXOL_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR6808851.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(4008..4943)
                     /gene="rihA"
                     /locus_tag="ACHXOL_001857"
     CDS             complement(4008..4943)
                     /gene="rihA"
                     /locus_tag="ACHXOL_001857"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR6808852.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5061..5786)
                     /gene="gltL"
                     /locus_tag="ACHXOL_001858"
     CDS             complement(5061..5786)
                     /gene="gltL"
                     /locus_tag="ACHXOL_001858"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR6808853.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5786..6460)
                     /gene="gltK"
                     /locus_tag="ACHXOL_001859"
     CDS             complement(5786..6460)
                     /gene="gltK"
                     /locus_tag="ACHXOL_001859"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR6808854.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6460..7200)
                     /gene="gltJ"
                     /locus_tag="ACHXOL_001860"
     CDS             complement(6460..7200)
                     /gene="gltJ"
                     /locus_tag="ACHXOL_001860"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR6808855.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7370..8278)
                     /gene="gltI"
                     /locus_tag="ACHXOL_001861"
     CDS             complement(7370..8278)
                     /gene="gltI"
                     /locus_tag="ACHXOL_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR6808856.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8733..10610
                     /locus_tag="ACHXOL_001862"
     CDS             8733..10610
                     /locus_tag="ACHXOL_001862"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR6808857.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(10688..12226)
                     /gene="lnt"
                     /locus_tag="ACHXOL_001863"
     CDS             complement(10688..12226)
                     /gene="lnt"
                     /locus_tag="ACHXOL_001863"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR6808858.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12251..13129)
                     /gene="corC"
                     /locus_tag="ACHXOL_001864"
     CDS             complement(12251..13129)
                     /gene="corC"
                     /locus_tag="ACHXOL_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR6808859.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13219..13686)
                     /gene="ybeY"
                     /locus_tag="ACHXOL_001865"
     CDS             complement(13219..13686)
                     /gene="ybeY"
                     /locus_tag="ACHXOL_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR6808860.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(13683..14723)
                     /locus_tag="ACHXOL_001866"
     CDS             complement(13683..14723)
                     /locus_tag="ACHXOL_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR6808861.1"
                     /translation="MNIDTCEITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(14876..16300)
                     /gene="miaB"
                     /locus_tag="ACHXOL_001867"
     CDS             complement(14876..16300)
                     /gene="miaB"
                     /locus_tag="ACHXOL_001867"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR6808862.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16446..17621
                     /gene="ubiF"
                     /locus_tag="ACHXOL_001868"
     CDS             16446..17621
                     /gene="ubiF"
                     /locus_tag="ACHXOL_001868"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR6808863.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(17775..17849)
                     /locus_tag="ACHXOL_001869"
     tRNA            complement(17775..17849)
                     /locus_tag="ACHXOL_001869"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17815..17817),aa:Gln,seq:ctg)
     gene            complement(17886..17960)
                     /locus_tag="ACHXOL_001870"
     tRNA            complement(17886..17960)
                     /locus_tag="ACHXOL_001870"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17926..17928),aa:Gln,seq:ctg)
     gene            complement(18009..18085)
                     /locus_tag="ACHXOL_001871"
     tRNA            complement(18009..18085)
                     /locus_tag="ACHXOL_001871"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18049..18051),aa:Met,seq:cat)
BASE COUNT         4417 a         4548 c         4732 g         4440 t
ORIGIN      
        1 cagttaatgc acttaaggga ttattaaaaa atgaataagg aagaacaata tcttcttttt
       61 gcgctttctg cgcccatgga aatacttaat cagagttgca aacccgcgca tgactcgcca
      121 aaaatgtata ctgggataaa agaattcgat ctctcatcat cctggggaat aaataatcgt
      181 gatgatttaa ttcagactat ttatcagatg actgatgatg gtcatgccaa tgatttagct
      241 ggcttatatc tgacatggca acgctcttcc cctgaagagt ggaaagcatt aattgctggt
      301 ggctcggaga gagggttgat ttatacccaa ttcgttgccc aaaccgctat gtgctgtgga
      361 gaagggggaa tcaaagcctg ggattatgtt cgaatggggt ttctgtcacg ggtcggtgtg
      421 ctcaacaact ggctgacgga agaagagagt ttatggctgc aatcgcgcgt ctatgcacga
      481 gctcaccatt actatcacag ctggatgcac tatttttctg cctattcact agggcgtctc
      541 tactggcaat cttctcagtg cgaagacaac gcttcgctac gcgaggcgct aaccctgtac
      601 aaatacgata gcgccgggag tcggatgttc gaagagctgg cagcgggaag cgaccgtttc
      661 tatgccacgc ttccctggca accattaact gtccagcctg aatgcccggt aacgcttaag
      721 gatgtgagcg acctatgaaa acatgttggc aaatcctcga aattgaaagc acgacgcaaa
      781 tagacattat ccgccaggct tatcttgctc gcttaccgtt gtgtcatccc gaaaccgatc
      841 cgcaggggtt taaagcatta cgccaggcct atgaagaggc gctgcgactg gcggtaaatc
      901 ctgtcgagga agcagatgat gaagaaaaag atgccaccgc tgaacatgaa atactacgtg
      961 cattcaggac attactggat tcagaaagtg atcgttttca gccctccgcc tggcagaaat
     1021 ttattcagca attaaatacc tggaacatgg aggatgtcga tcaattacgc tggccgctgt
     1081 gtgcaatcgc tatagaagcg cgatatcttt cattaaattg tgcttctttg ttggcagagc
     1141 gtttgaactg gcattcgttt aatgacagcg aaggaatgga tgaggaagaa agggaggctt
     1201 ttcttgaggc cattcaggct ggtgattgtt tcgatttcct tagccttctg gaatatcccg
     1261 tcgcgttaca gaaccagact gttgaatatt acttcgcgct ggaacgttgc tgccgttacc
     1321 atcctgacta tgtcactgcg tttttggcga tggaaggacc gtggttcatt cctgatgatg
     1381 caaagttaca tcgcaaactg ttgcgctggt acagctcggt gcaaacaggt atggcggaac
     1441 tcattcctgt agccaaacag tggcaagcgg aagaaccaga aagcgaagat gcccggtatt
     1501 accagtgtgc acaacgtttg tactgcggcg agggggaaag cctgcttgcc gatctctgcg
     1561 cgtactggga aagttaccca tctacacaag ctgataattt gttgttgcag tggagcaagc
     1621 gtcattgccc ggattatttc gcgttattag tgatggttat cgaagcgcga agcatggtag
     1681 atgcgcaagg taaaccgttg aaatatgttc ctggtgagag cgcccggacg cggctgttat
     1741 gggcggagat tttacatagc ggaaaattat cgccgttagg tcaatcgttt attgagtcgt
     1801 tattcttcaa gcgcaaagca tgggcgtggt ggaaatcgag agtcggtagc gagacagagc
     1861 aagattcacc gttactggat ttgtatcggg tagcggaaca ggttgtgctg gaagcgttcc
     1921 cgaaacaaga gatgctggcc cgtcttaata caaggctgga aggtggagat gctcatccat
     1981 tagaggccat tatcacccag atgctgttga cgaaagtgaa actcgagccg gaggatgaag
     2041 atgtcgatga gccaacacct gaaaaccatg aagaaaaaaa tgatgaggat gaaaaaccac
     2101 agagcattac cagtattatc aaaatcagct taacggtgct gatgataggt tatgcgctcg
     2161 gaaaaatcgc gatgttgttt agctgaggat gtgttttttg cgccgatgcc ggatgcagcg
     2221 tttatgccgc atccggcagc cgtgtttaag ggatctcaat agccaaataa tcgctcgccc
     2281 gtcgacgaac ttcatcgacg cgggccatat cgtttgactg caacgccgca tcaaaatcgg
     2341 tgatcacccg gccaatctct tctcgctcgt cacccagcgc ccgcgcccac aactcttcca
     2401 gtttggcctt aaaggtgcgg ttgattagca tatcgcgcgg ataaattttc aatgcggata
     2461 accgcgtccg actctcttca atctgctgcg cgctcaatgt taccgggcta tgattaatga
     2521 ctctggactt aacgctgcca tcttccagaa gcacgtcaac ttcaagcaag ccgttgatat
     2581 cataactaaa gcgaatatca atcgactgat aagccccggt tttcttcaac ggcacatcga
     2641 aggattcaac cagaatgtta tttttaactt tgtggttttc tccctgatag acgttaaccg
     2701 taatagaatc ctgttccggg tgcatggtcg agtaagtttc tacacgcgac acgggtactg
     2761 tggtgtttcg ttcaataatt ggcgagaaaa tgccggaaac gccctggcgg ttaacttcaa
     2821 cgcccaacga gtaagggcaa atatcagtga ggatcacctc ttcaatatct tcactgcgta
     2881 agcggcaggc ggcctggatt gctgcgccca gcgcgacaat ggtgctcggg tcgtaacttt
     2941 gatacggtaa tttgccaaac agacgcacgg cgattcgctg caccagcggc atttgtgacg
     3001 cgccgccaac cagcaccaga ctgtcgattt gactcggctt cagacgtgca tcgcgcaacg
     3061 cctgttcaat cggcactcgc aagcgattga gcagcggcaa ccacaaatct tccagttcgt
     3121 tctcgtaaaa ttcgcactcc cgcgtttcat cctggtactg ccagcgaatg tgcaatggcg
     3181 attgattgct acattttgcc gcttccacac aggcgtacaa ggctgccagt tcactctcgt
     3241 taagcgtggt cctggcgaca tccgcgcgct ttaaaacgtc atcgaccaac atatgggtaa
     3301 aatcttcgcc accgaggaag ttatcgccag cggaggcgtg aacttcaatc accggcgtgg
     3361 cgtattcgag caccgtgacg tcaaacgtgc cgccaccgag atcaaaaacc agcgagcggg
     3421 tattttgttg cgtatgcagg ccatatgcca tcgcagctgc tgtgggttca ttaattaagc
     3481 gtaccgcatt taacccggct aactccgctg ctaaacgggt atgcttgcgt tgttcatcgc
     3541 tgaaataagc cggaacggag atcaccacat ctttaatcgg acgttgcaga aattcttcgg
     3601 catcttcttt taatgagcgt aataccaaag aggacagttc tggcgcgtta aatgtgtcgc
     3661 tgcctaaccg ccagttggta ttactgccca ttgcacgttt aaataacgct gccgttttat
     3721 ccggatgcga agtacgccgt gatacagccg gttttccgac taaaatatga ttattttcat
     3781 ccatgctaat tatggatggt gttaaatatt caccgaactt atttggaatt aattgcgcgg
     3841 caccgtcttt ccagacggca attaaactat tggtagtgcc gagatcgata ccaatggcga
     3901 gttctgcatt atccattgca tttatatctt gaaagaaaaa gagataaaca gataaacaga
     3961 ttaagaccac ccaggtaacg cgtggtgatc ttaatcaatg acgtgtgtta agcgtaaaat
     4021 ttcagacgat cagccagtaa atcaacaaag ccctgacgat caacatcgac cattacagtg
     4081 gcattcggtt tattgccagt cagataataa taatcaacaa ccgtcatacc ctggctatat
     4141 ttcccctgtg tttccacgcc aacccagcgc tcaacagtgg taaacaactc gggtttcaac
     4201 agccaggcga tggtgcatgg gtcatgcagc ggtgcaccga caaagcccca tttttcgtct
     4261 ttatgatatt cgaggaagaa atccagcagt tcggcaacaa tggttgaaac agggttgcca
     4321 atcgcgcgga aacgctcggt gtcttcaacg tggatttgtg ctttgtgagt aacatccaga
     4381 ccggccatca ccactgggat ccctgactgg aagacaattt ctgccgcttc cgggtcaacg
     4441 taaatattaa attcagccgc aggcgtccag ttaccaagcc ccattgcgcc gcccataatc
     4501 acgatacggg caattttgct atgcagttcc ggatggctat tgagcagcaa ggcaacgtta
     4561 gtttgcggtc cggtagacac aatcgtgaca ggttccgcac tttcgcgcag cgttttcgcc
     4621 atcagctcta ccgccgtaca gttttgcggt gcgaacgtcg gttccggtaa tgccgggcca
     4681 tccagaccgc tttcgccgtg cacattgtcc gcgataatca actcacgcat taacggtttt
     4741 accgcgccgc ctgctaccgg aatatcggtg cgattaagca aggtcagcat acgcagaaca
     4801 ttgcgtaagg ttttttctgg tgtctggttt ccggcggaag acgtaattgc tttgacatca
     4861 agctctggtg aggcgagggc gagaactatt gcgatagcgt cgtcatgacc tgggtcgcaa
     4921 tctaacagaa ttggcagtgc cattgttgct ccttgttgtg tgcttctttg cgacaagggt
     4981 aacgccagga tgtaacagat acgaggggcg aaacgataaa gcgtgagatg gcgcgcactt
     5041 gggtatgcgc gccagagtga ttaatgcagg attttcgcga ggaagtcttt cgcgcggtcc
     5101 gatttcggat catcgaagaa agcgtctttc ggcgagtctt cgacaatttt accctcgtcc
     5161 ataaagatca cccgattcgc cactttgcgg gcaaagccca tttcgtgggt caccaccatc
     5221 atggtcattc cttcgttcgc cagttccacc atcacgtcca gcacttcgtt gatcatctcc
     5281 ggatccagcg ccgatgtcgg ttcgtcgaac agcatcgcaa taggatccat acacaatgcg
     5341 cgagcgattg ccacacgctg ctgctgaccg ccggaaagct gcgccggaaa tttattggca
     5401 tgtgcggaaa gtcccacgcg ctccagcaat ttcaatgctt tttcacgagc gggcgcttta
     5461 tcgcgtttaa gcactttcac ctgcgccagg gtcaggtttt cgataatcga cagatgaggg
     5521 aacagctcga aatgctggaa taccatcccg acgcgggaac gcagctttgc cagatcggtt
     5581 ttcttgtcat taaccacgat accatcgacg gtgatttcac cttgctgcac tggttcgagg
     5641 ccgttgacgg ttttaatcag cgttgatttg ccggaaccag acgggccgca aaccaccacc
     5701 acttcgcctt ttttcacttc ggttgagcag tcggtcagca cctgaaagtg accataccat
     5761 tttgaaacat ttttcagggt aatcattatg ctgtccttct tttcaagtag ctgaccaaca
     5821 acgacgcgct aagactaata acgaaataaa caaatccggc aaacaggatc atctcaacct
     5881 gcgtaccatc acgctcacca atggttgagg cggtacggaa gaaatcggcc agagataaca
     5941 catacaccag tgaggtatcc tggaacagta cgatgccctg agtgagcaga agcggcacca
     6001 tcgcgcggaa cgcctgcggc agaataatca gtttcatcga ctgccagtga gtcattccca
     6061 acgccagcgc ggcgctcgac tgaccacgag aaatactttg aataccagca cggataatct
     6121 ctgaatagta ggccgcttca aacatcgaaa acgccaccat cgccgaaatt aaacggatat
     6181 cattttttgg tgataatccc agcacgtttt gcagaaaacc tggcacgatc aggtaaaacc
     6241 acagcaaaac cataactaaa ggaatcgagc ggaatacgtt aacgtaggct ttggcaaacc
     6301 acgctacggg cgcaaagctg gacaagcgca tcaccgccag catggtgccc cacaaaatac
     6361 caatcacgac tgccgtgacg gtgattttca gcgtgatcac cagcccgtcg agcagatatg
     6421 gcagggaagg gacaatggaa ctccagtcaa actcgtacat tatttgcccc ccatgttgcc
     6481 aggcaggcga actttacgtt caaccagcgt cattaccagc atgataaccg cgttaatcaa
     6541 cacatacgcc agcgtaatag cggtaaacga ctcccaggcg tgggctgagt aatcaagcaa
     6601 tttacccgcc tgcgccgcca tatccaccag accaatagtc gaggcgatgg cggagttttt
     6661 caccaggttc atcatctcgg aagtcatcgg cgggacgata acgcgataag cattaggcag
     6721 cagtacgtat cgataagcct gcggtagcgt caggcccatc gccagcgcgg catttttttg
     6781 cccacgcggc aacgactgaa tggcggcgcg tacctgttcg caaacacggg cggcggtaaa
     6841 cagccccagg cagagcatgg atgaaaggaa aaactgaata ttgggatcca gctcggcctt
     6901 aaaccacatg ccgattttct ccggcaacag ctccgggatc accagatacc aggtaaagaa
     6961 ttgcacaatc aacggcacgt tgcggaacag ttcgacatac aacgtaccca gaccggagag
     7021 aaaacggttt ggaacggtac gtaaaatgcc gaaaaatgaa ccgacgagga aagcgataat
     7081 ccaggcgcag atcgacaaag cgatcgtcac ctgaaaacca ctccagatcc aaccaagata
     7141 ggtggtgttg ccgaacgggg cttgttgtaa aaaaataccc cagttccagt ctatagacat
     7201 aaatctactc cagaaaaaag agggtagcag cgttaactgc taccctcgaa gattgttacc
     7261 cagcgtattg cggttttcag gcccgatggg gaacgaccat caggcgtata gtctgtccgt
     7321 gctacgtaac aatcgagagg gctggaattt ccgcccctgg ttcttgtaat tagttcagtg
     7381 ccttgtcatt cggttctttg aacagtgctt tcatttcgtc tgacagttcg aaattcatgt
     7441 tcaggttttt cggcggaatg ggatttttga accatttatc aaaccatttt tccgcttcac
     7501 cggaggtctg cacctgcgcg gtggtgtcat ccatcagctt tttgaactgc ggatcgtctt
     7561 tacgcaacat gcaaccgtag gcttcctgag actgcggctt gccgacgatt tcccagttgt
     7621 ctggtttctt cgctttcgca cgttcaccgg ccagcagggc atcatccatc ataaaggcaa
     7681 cggcacgacc gctttccagg gtgcggaaag agtcactgtg atctttggcg ctgatgatgc
     7741 gcatattcat tttttgctct tcattcagtt tgttgagcaa aacttcagag gtagtgccgg
     7801 aagtgacgac tacggctttg tctttcaggt cggcaaaatc tttgatatcg ccgccctttt
     7861 tggtcagcag gcgcgtaccg accacgaaaa tagtatcaga gaaagccgcc tgtttttggc
     7921 gttcgacgtt gttggtggta gaaccacatt caaaatcgaa agtgccgttt tgcagcagtg
     7981 gaatacggtt ttgtgaggta atcggaatca gttttacctg caagtccggt ttgttgagtt
     8041 tctttttcac tgcttcaaca atggcgttgg agtaatcctg cgagtaaccc accacttttt
     8101 gctgattgtc gtaataagag aaaggtactg aagattcacg gtgaccgacg acaatcacac
     8161 cgtttttggc gattttgtcc agcgtactgc ctgctgccgg ggcggcgtca tctgcctgtg
     8221 ccagtcctgc ggaaagcgcc agggcgagga ttgctgtggc aggtttacgt aattgcatat
     8281 ccaactcctt tatcttctgc gttaagaacg catggatacc cgttgtgagt gtttgtgttg
     8341 ttatcgtctg caactttatt gtgcagtgtt gtgcctgtta ggtaacattt agtttggcta
     8401 aatgtaaaga tattgctgtt ttattgtttg tttttgcgag atgcgccgca ccattccgaa
     8461 gcaaaattct taaaccgcac ctttttggtg ctaccgctga tttgtggtgg tgcaacctgg
     8521 ttgcgtcgcg gctgatttca tcgcttacac agtaaataaa gcaacttaca tgccaaaaaa
     8581 tacttagggg cgaattaatt acgattgtaa ttttaggctg aaaaagtcat atttcggaca
     8641 gaattatctt ttcggcatgg gacgtttgcc gggatgataa ctgcacacgg gaaaaatgct
     8701 ttttacctgt cttttcaagg aagaaacacc ttatgtctgc atcttcggtt aagccgttaa
     8761 atgttcaatt acccgcaata acccttatcc tttttgcact ctgtattggg atattttgtt
     8821 atctcgcaca atggatgagt tatgaagagg tcgatcaatc cgcacttatc catctcggcg
     8881 caaacgttgc gcctcttact ttgtcgggtg aaccctggcg cttattgagc agtatctttc
     8941 tgcacagtag tgtttctcat ctgctgatga atatgtttgc attcctggtc gtgggaggcg
     9001 tggcggaaca gattctgggg aaatggcgac tcctgattac ctggttattc tccggcgtct
     9061 ttggtgggct catcagcgcc tgttatgcgt tacgcgaaag tgagcagata gtcatcagcg
     9121 ttggggcatc cggggcgatt ctgggaatag ctggtgcagc gatagcaaca cagtttgctt
     9181 caggtacggg cacataccat aaaaaccagc ggcgagtatt tcctctgttg ggtatggtgg
     9241 cgctgacact gttgtacggt gcccggcaaa caggaataga taacgcttgc cacattggcg
     9301 gcctgattgc gggtggcgcg ttgggttggc tgagcgcgcg tttatctggg caaaaccgac
     9361 tcgttacgga aggcgggatt attgttgcgg gcagtcttct tctgaccggg gctatctggc
     9421 ttgcgcagca gcagatggat gagtcagttt tacaggtcag gcaaagcctg cgtgaagcgt
     9481 tttatccaca ggagattgaa caagagcgac ggcaaaaaaa gcagcagtta gcggaggaac
     9541 gcaacgccct cagggaaaca ttatccgctc cggtaagtcg tgaacaggcc agtggtgatt
     9601 tgctcgctga gattgccgat atccatgata tggcgatcag tcgggatggt aatatgttgt
     9661 atgccgcaat tgaaaacacg aacagcattg ttgttttcga cctcggacaa aagaaaatcc
     9721 tgcatacctt tacagcgccc atagcgaaag aaaagtcagt caaacattgt ggtggctgta
     9781 aagatcaggg cgtcagatcg ctggcattaa gcccggatga aaagttgatt tatgcgactt
     9841 catttgaagc gaatgcgtta tcggtcatta acgtggcgac aggggagatt attcagtcga
     9901 ttaccaccgg tgcacatcct gacagcctta tcctctcgcg tgatggcaca aaagcctggg
     9961 tgatgaatcg caccagtaat agtgtgtcag cgattgatct ggtgacttat cagcatgtgg
    10021 cggatatccc gctggagaaa tacgacggga cggggacgag cggtaaacca ggcgcctggg
    10081 ttatggcact ttccccggat gaaagaacat tactggttcc gggagcaggc agaggtaaca
    10141 tcgtgcggat caataccatc acgcatcaga aagaagactt tcccgcaggt gatgcgcgtg
    10201 gaacgatatc ggcgatgcgt tttcgacctg aaaacggcga ggttattttt gccgatagtc
    10261 aggggatttc acgtataagc gtaggggatc aacaagccag cattatgacg caatggtgta
    10321 gcaggagcgt ttattccgtt gagggtatta gcccggacgg tcaatattta gcgttggtgt
    10381 catatggcct gcaaggttat gtcatcctgc tcaatattaa tgccgggcag attattggcg
    10441 tttatcctgc cagctacgtt aatcaccttc gtttttcagc ggatggtaga aaaatatttg
    10501 tcatggcgaa gaacgggttg atccaaatgg acaggacgcg ctcgcttgat ccgcaggcaa
    10561 ttattcgtca tccccaatat ggcaatgtgg cttgtatccc tgaaccgtaa gaaaaagggg
    10621 catgataacc atgccccaaa gctcaatgag aaagtacgtt ttgctcatgc aggatacatc
    10681 aggcacatca acgtttacgt gctcgcagac tcatcaacac agcggcaaaa ccaaacagcg
    10741 ccgtcagcac ccacagtggc cagttgccgg tacgtgcata cggtgtgagt ccggtggtcg
    10801 gtgtcacatt agtggtcaac acctcgcggg tgaactgcgg gatcatcgcc tgaacctccc
    10861 cctgcgggcc aatcaccgcc gtaatgccgt tgttggtgct gcgcaacagt gggcgcgcca
    10921 gctccagcgc acgcattcgc gccatctgga agtgctgcca tggaccaata gacttaccaa
    10981 accacgcatc gttggagata gtcagcagat agtcggtatc cgggcggaag ttatcgcgca
    11041 cttgctcgcc gagaatgatc tcgtagcaaa tagccgcagt aagctcaata ccatttaccg
    11101 acagcggcgg ctggatatat ggcccacggc tgaacgacga catcggcaga tcaaagaacg
    11161 gtgctaacgg acgcagaatc gactccagcg ggacaaactc gccaaacggc actagatggt
    11221 ttttgttata gcgatcggct gattcgtagc tgtatggtgc acctttaccc agtgtgatga
    11281 tggtgttgta ggtatcgtag cggttctgct tattgagacg cgcgtcgaca atcccggtta
    11341 ccagcgagct acctttatcg cgcaactcac cgtccagtgc tttgaggaac ggttgctgat
    11401 taatttccag atcggttatc gccgactccg gccagataat caacgatgat ttgcccatca
    11461 gcggagccgt tgcgttgtag taaatcttca gcgtattaag aagctgatct ccatcccatt
    11521 tcagtgattg cggaatatcg ccctgaacca tcgaaacctg aatggttttc tccggttgcg
    11581 gggtaaacca ttggatgtaa cgcagcggga agggtagggc aaacagcacg acggccacca
    11641 ccagcggacg ccagttgcgt ttgaccaacg ccagtgccag caggccacta accatcatca
    11701 gcaggaagtt aatggcttcc acgcccatta tcggtgccag cccttttaac gggccatcaa
    11761 tctggctata gccgaactgt aaccacggga agcctgtcag tacccaaccg cgcagaaact
    11821 cggtcacttg ccagagagca ggggcggcaa tcgccacgcg cagccaggtg gttttcggcc
    11881 acagacgcga cagcacgcca gcaaacagtc cggtatacag cgacaaatac gccgccagca
    11941 gcaccaccag gaagatgtta accgggccag gcattccgcc aaaggtcgcg atgctgacat
    12001 agacccagtt aataccgctg ccaaagaggc caaatcccca gcaaaagcca atagcggcag
    12061 actggagtgg acggcggtta aaggtcaacg cctgaagccc catcaacgaa ataatcgccg
    12121 caggccagac gtcgtaagga gagaaggcca gcgttccgca ggcaccgaat aataacgcca
    12181 gcagcaggcg aatgcgctgg cgttcaatta atgaggcaaa agccatgtag ttatctatcc
    12241 agtttcggtt ttattcatcc agcttcggct ggggtgagtc atccgggatt ttgacatgaa
    12301 cctgaataat acgccgactg tcggccatcg ccactttgaa ctggtaaccg tcgatgtcga
    12361 tagtttcgcc acgcgccgga agatgcccaa atgcctgcat caccagacca ccgatagtgt
    12421 caacctcttc atcgctaaag tgggtgccga acgcttcgtt gaagtcttca atggaagcca
    12481 gtgcacgcac ggtccaggta tgacgactca gctgacggaa gtcgatatca tcttcttcgt
    12541 catactcgtc ttcaatctca ccaacaatca gttccaggat gtcttcaatg gtcaccagac
    12601 cggaaacccc accgaattcg tcaatgacga tcgccatgtg gtaacgctga gagcgaaact
    12661 ctttcagcat ccggtctacg cgcttacttt caggaacgac aaccgcctga cgtaacactt
    12721 tgtccatgct gaaggcttca gcatcgctgc gcataaacgg cagcaagtct ttcgccatca
    12781 gaatcccttc aatgtgatct ttgtcttcgc taatcaccgg gaaacgtgag tgggcggact
    12841 cgatgatgac atcaaggcat tcgtccagcg tctggttgcg tttcagggta atcatctggg
    12901 agcgggggat catgatgtcg cgaacgcgtt ggtctgcgat gtccatcacc ccttcgagca
    12961 tatcgcgcgt atcttcgtcg ataaggtcgt tctgcccgga atcacggatc agcgccagca
    13021 gttcgtcacg gtttttcggt tcaccgtgga aaagttggct gagtaacagg gagaaaaatc
    13081 ccttcttgtt gcttatcgtg tcactactgt gtgaattgtc gtcgctcatg gcgtcgtatg
    13141 ggttctcatg ttagttaatc aaaacgccgt cgttaatcac caacggcggg gacgtctgcc
    13201 agtcaaatgc ctggcaaatt attctttctc ggcaatgtac ggatcctcat agcccagagc
    13261 aagcataatc tctgtttcga gggcttccat ttcttctgct tcgtcatctt cgatgtgatc
    13321 gtaacctaac aaatgcagac tgccgtgcac caccatatgc gcccagtgcg cctccagtgg
    13381 tttgccttgc tcctgagctt ccttctcaac cacctgacgg cagataacca gatcgcccag
    13441 tagcgacatt tccatgccag gcggcacttc aaacgggaag gagagcacgt tggtcggctt
    13501 atccttaccg cgataggtca gattcagact gtggctttcg gcggtatcga ccacgcgaat
    13561 agtcacttcc gattcttcct gaaactgcgg gatcaccgca ttcagccatg tttgaaactg
    13621 gctctcttcc ggtaacccgg aattatcttc acatgccagt tgtaaatcga ggatcacctg
    13681 actcattttt gttcctgttc ttcgcgcttg cgttctgccg ccagcgccgc ttttcgtttt
    13741 tgttcggctt cttcccaggc ttcataggcg ttaacgatac gcgccaccac agggtgacga
    13801 accacgtctt cgctgtggaa gaagttaaag ctgatctctt cgacatcggc cagcacttcg
    13861 atggcgtgac gtaagcctga tttagtatta cgcggcaggt cgatctgtgt gacgtcgccg
    13921 gtgataaccg cttttgagtt aaaaccgata cgggtcagga acatcttcat ctgttcgatg
    13981 gtggtgttct ggctctcatc gagaatgata aacgcgtcgt tcagcgtacg accacgcata
    14041 taggccagcg gcgcgacttc aataacgttg cgctcaatca gtttctcaac tttctcaaag
    14101 cccagcattt caaacagcgc gtcgtacagt gggcgcagat acgggtctac tttctggctt
    14161 aaatcgccag gcaggaagcc cagtttctca ccggcttcga ccgccggacg agtcagcaga
    14221 atacggcgaa tctcctgacg ctccagggca tcaactgccg cagccactgc caggtaggtt
    14281 ttacccgtac ccgccgggcc aacgccgaag gtaatgtcat ggtcgagaat attggcgatg
    14341 tactgcgcct ggtttggcgt gcgcggctta attacgccgc gtttggtttt gatattgacc
    14401 gctttgccgt actccggcac gctctccgca ctttgctcca gtactcgcgc ttctttaatc
    14461 gcaaggtgga tctgttccgg ttcgatatcc tgaatctgac cgcgcatcgg ggctgtatcg
    14521 acatacaggc tacgcagaat gtctgccgca gcggtgacgc aaatcggacg gccggtcagt
    14581 ttaaagtggt tgtcgcggcg attgatctcg atgccgagac ggcgttcgag ctgcttgatg
    14641 ttgtcatcaa acgggccgca caggctcaac agacgcgcgt tgtctgctgg ctccagggtg
    14701 atttcgcaag tgtctatgtt caaaccgttc ctcttatctg tatgccgccg gaagctgaac
    14761 attcaccggc ctataaggaa attattcacg ccacaggaaa aaggcgcaag cattgcgata
    14821 aagatgggga taaagagaga aaaaacaagg cccaccggaa cggcgggcct gagaattacg
    14881 gctgataata acccacgcca aggtcgtttt ctttgcgggt acgggcaatc accgattccg
    14941 gtgtttctgc cacgcgcaga cccatttcat cttcagtacg caccacttta ccgcgcagag
    15001 agttcgggta gacgtcggta atttctacat cgacgaattt accgatcata tccggtgtgc
    15061 cctcgaagtt gaccacgcgg ttattttcgg tacgcccgga aagctccatg atgctcttac
    15121 gcgaagtccc ttctaccaga atacgctggg tggtgccgag catccggcgg ctccatgcca
    15181 tcgcttgctg attgatacgt tcttgcagaa tatacagacg ttgcttcttc tcttcttccg
    15241 gaacatcatc aaccatatcg gcggctggtg tacccggacg tgcagagaag ataaagctgt
    15301 agctcatgtc gaaattgacg tcggcaatca gcttcatcgt tttctcgaag tcttcggtgg
    15361 tttcgccagg gaagccaaca atgaagtcag aactgatctg aatatccgga cgcgccgcac
    15421 gcagtttacg gatgatcgct ttgtactcca gcgccgtgtg agtacggccc atcaggttca
    15481 gaatgcgatc ggaaccgctt tgtaccggca gatgcaggaa gctcaccagc tccggcgtgt
    15541 cgcgatacac ttcgatgata tcgtcggtga attcgatcgg atggctggtg gtaaagcgaa
    15601 tacgatcgat cccgtcgatc gcagcaacca gacgcagcag atcggcaaac gatccggtgg
    15661 agccgtcgta gttttcacca cgccaggcgt tcacgttctg accgagcagg ttgacttcac
    15721 gcacgccctg agccgcaagc tgggcaatct caaacagaat atcgtcagac ggacggctta
    15781 cctcttcacc acgggtgtaa ggcaccacgc agtaggtgca atatttattg cagccttcca
    15841 tgatggagac aaacgcggtc ggcccttcgg cgcgcggttc cggtagacgg tcaaacttct
    15901 cgatttccgg gaagctgata tctacaaccg ggctgcggtc gccgcgcacg gagttgatca
    15961 tctccggcag acggtgcagc gtttgcggcc caaaaataat atcgacatag tgggcgcgct
    16021 ggcgaatgtg ctcgccttct tgcgatgcca cgcagccacc gacgccgata atcaggtctg
    16081 gattcttctc ttttaacagt ttccagcgac ccaactgatg gaagactttt tcctgagcct
    16141 tctcgcggat tgagcaggtg ttcagcagca gcacatccgc ttcttccgcc acgtcggtca
    16201 gttgatagcc gtgggtggca tccagcagat cggccatctt cgatgaatcg tactcgttca
    16261 tctgacagcc ccaggtttta atatggagtt ttttggtcat cgacttgctc ttgcgaaata
    16321 gtagccagga atgcagggcg tatagtgtaa tgctttgctg ccgttgtgac cagtatgagc
    16381 gttatcagcc cttgggggta aaaatcctgt aaacttaaag catattgcta acaggatgat
    16441 tgaccatgac aaatcaacca acggaaattg ccattgtcgg cggaggaatg gtcggcggcg
    16501 cgctggcgct ggggctggca cagcacggat tttcggtaac ggtgatcgag cacgcagaac
    16561 ctgcaccgtt tgtcgctgat agccaaccgg acgtgcggat ctcggcgatc agcgccgcat
    16621 cggtatcatt gcttaaaggg ttaggggtct gggatgcagt acaggctatg cgttgccatc
    16681 cttaccgcag actggaaacg tgggagtggg aaacggcgca tgtggtgttt gacgccgctg
    16741 aacttaagct accgttgctt ggctatatgg tggaaaacac cgtcctgcaa caggcgctgt
    16801 ggcaggcgct ggaagcgcat ccgaaagtaa cgttacgtgt gccaggctcg ctgattgcat
    16861 tacatcgaga taatgatctt caggagctgg aactgaaggg cggtgaaacg atccgcgcga
    16921 aactggtgat tggtgccgac ggcgcaaatt ctcaggtgcg gcagatggcg ggaattggcg
    16981 ttcatgcctg gcagtatgcg cagtcgtgca tgttgattag cgtacagtgt gagaacgatc
    17041 ccggcgacag cacctggcag caatttaccc cggacggacc gcgtgcgttt ctgccgttgt
    17101 ttgataactg ggcatcgctg gtgtggtatg actctccggc gcgtattcgc cagttgcaga
    17161 atatgaatat ggcgcaattc caggcggaaa tcgcgaagca tttcccgtcg cgtctgggtt
    17221 acgttacacc gcttgccgct ggtgcgtttc cgctgacgcg ccgacatgcg ttgcagtatg
    17281 tgcagccggg gcttgcgctg gtgggcgatg ccgcgcatac catccatccg ctggcggggc
    17341 agggggtgaa tcttggttat cgtgatgtcg atgccctgat tgatgttctg gtgaatgccc
    17401 gcagctacgg tgaagcgtgg gccagttatc ctgtcctcaa gcgttaccag atgcggcgca
    17461 tggcggataa cttcattatg caaagtggta tggatctgtt ttatgccgga ttcagtaata
    17521 atctgccgcc gctgcgtttt gtgcgtaatc tcgggttaat ggcggcagag cgtgctggcg
    17581 tgttgaaacg tcaggcgctg aaatatgcgt tagggttgta gccttacaac attgccggat
    17641 gcgtgccaac cgtaggtcgg ataagacgcg gcagcgtcgc atccgacatt gaaggataag
    17701 acgtgtcaac atcgcattcg acattgaatg aacgcagaaa agcaaaaaac tcgccgaagc
    17761 gagctttttt aatgtggctg gggtacgagg attcgaacct cggaatgccg gaatcagaat
    17821 ccggtgcctt accgcttggc gataccccaa ctggtgcact tacgatgtaa gcgtcttgaa
    17881 taaattggct ggggtacgag gattcgaacc tcggaatgcc ggaatcagaa tccggtgcct
    17941 taccgcttgg cgatacccca acaaattggt ttttgaattt gccgaacgta ttcgacacat
    18001 tcagaatttg gtggctacga cgggattcga acctgtgacc ccatcattat gagtgatgtg
    18061 ctctaaccaa ctgagctacg tagccagatt gtttcttcga tggctggggt acctggattc
    18121 gaaccaggga atgccgg
//
LOCUS       DAZRUF010000069        17994 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZRUF010000069 DAZRUF010000000
VERSION     DAZRUF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.069"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            214..519
                     /locus_tag="ACHXOL_001872"
     CDS             214..519
                     /locus_tag="ACHXOL_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808864.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            626..1270
                     /gene="gfcB"
                     /locus_tag="ACHXOL_001873"
     CDS             626..1270
                     /gene="gfcB"
                     /locus_tag="ACHXOL_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR6808865.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1267..2013
                     /locus_tag="ACHXOL_001874"
     CDS             1267..2013
                     /locus_tag="ACHXOL_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR6808866.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2013..4109
                     /locus_tag="ACHXOL_001875"
     CDS             2013..4109
                     /locus_tag="ACHXOL_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR6808867.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSQLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4155..5294
                     /locus_tag="ACHXOL_001876"
     CDS             4155..5294
                     /locus_tag="ACHXOL_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR6808868.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5282..5728
                     /gene="etp"
                     /locus_tag="ACHXOL_001877"
                     /gene_synonym="yccY"
     CDS             5282..5728
                     /gene="etp"
                     /locus_tag="ACHXOL_001877"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR6808869.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5748..7928
                     /gene="etk"
                     /locus_tag="ACHXOL_001878"
     CDS             5748..7928
                     /gene="etk"
                     /locus_tag="ACHXOL_001878"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR6808870.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8043..9341)
                     /gene="appA"
                     /locus_tag="ACHXOL_001879"
     CDS             complement(8043..9341)
                     /gene="appA"
                     /locus_tag="ACHXOL_001879"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR6808871.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9421..9513)
                     /gene="cbdX"
                     /locus_tag="ACHXOL_001880"
     CDS             complement(9421..9513)
                     /gene="cbdX"
                     /locus_tag="ACHXOL_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR6808872.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9526..10662)
                     /gene="appB"
                     /locus_tag="ACHXOL_001881"
     CDS             complement(9526..10662)
                     /gene="appB"
                     /locus_tag="ACHXOL_001881"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR6808873.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10674..12218)
                     /gene="appC"
                     /locus_tag="ACHXOL_001882"
     CDS             complement(10674..12218)
                     /gene="appC"
                     /locus_tag="ACHXOL_001882"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR6808874.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12352..13209)
                     /locus_tag="ACHXOL_001883"
     CDS             complement(12352..13209)
                     /locus_tag="ACHXOL_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR6808875.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13266..13604)
                     /gene="hyaE"
                     /locus_tag="ACHXOL_001884"
     CDS             complement(13266..13604)
                     /gene="hyaE"
                     /locus_tag="ACHXOL_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR6808876.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(13601..14188)
                     /gene="hyaD"
                     /locus_tag="ACHXOL_001885"
     CDS             complement(13601..14188)
                     /gene="hyaD"
                     /locus_tag="ACHXOL_001885"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR6808877.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGRLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14185..14891)
                     /gene="hyaC"
                     /locus_tag="ACHXOL_001886"
                     /pseudo
     CDS             complement(14185..14891)
                     /gene="hyaC"
                     /locus_tag="ACHXOL_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(14910..16703)
                     /gene="hyaB"
                     /locus_tag="ACHXOL_001887"
     CDS             complement(14910..16703)
                     /gene="hyaB"
                     /locus_tag="ACHXOL_001887"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR6808878.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16700..17818)
                     /gene="hyaA"
                     /locus_tag="ACHXOL_001888"
     CDS             complement(16700..17818)
                     /gene="hyaA"
                     /locus_tag="ACHXOL_001888"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR6808879.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4389 a         4806 c         4692 g         4107 t
ORIGIN      
        1 ttgctaagat ttatcttatg ctcacaagtc gtatttccag aggggatcgt taataatctt
       61 atatttccct ctgcgcacct ttcctacccg cagtatcagt atatagtgca agcggtatga
      121 ggaaagggag atttcacaag gatgttatac tcaaagtaga caatcctgat ctcaagcgta
      181 cgtattgtcc atgcgatgaa taaaggaaaa gttatgaaac acaagctttc tgcaatcctt
      241 atggccttca tgttgacgac gccagctgcg tttgctgcct ctgaagcaac aaacgggacc
      301 gaggcaacga cgggtactac tggcaccaca acgaccacta ccggcgcaac cacgactgct
      361 actaccactg gtggcgtggc tgctggcgct gtcggcacgg cgacagttgt cggtgtggca
      421 acggctgttg gtgtggcgac gttggcagtt gttgcagcaa atgacagtgg cgatggcggt
      481 tctcataaca cctcaaccac cacgtccact acacgctaat actgctttaa cgcgtcgaaa
      541 ttaaagttaa atgatacccg gttgcgatat tcgtaccggg ctttagagat ttgcattttt
      601 ttaattaacc tttcaaggat taaaggtgcg ccctcttatt ttatcgattt tcgcactatt
      661 tcttgcggga tgtacgcaca gccagcaaag tatggtcgat acatttcgcg ccagcctttt
      721 cgataatcag gatatcaccg tagcggatca gcagatccag gcgttgcctt attccacgat
      781 gtatttacgc cttaatgaag ggcaacgaat ctttgtggta ctgggatata tagaacaaga
      841 acaaagcaaa tggttatccc aggataacgc catgctggtt acccacaatg gacgtctttt
      901 aaaaaccgtc aaacttaata ataatctgct ggaagtgaca aattccgggc aggaccctct
      961 gcgtaacgcg ctggcaataa aagatggcag ccgctggacg cgcgatattc tctggagtga
     1021 agacaaccat tttcgctctg cgaccctgag ttctactttt tcctttgctg gattagagac
     1081 gctgaatatt gcgggtcgca atgtgctgtg taatgtctgg caggaagagg tgacttccac
     1141 gcggccagaa aaacagtggc aaaacacatt ctgggtcgat tcggctactg gccaggttcg
     1201 tcaaagtcgg caaatgttag gcgcaggggt tattcccgta gaaatgacgt ttcttaaacc
     1261 cgcaccatga ataaattaca gtcgtatttc attgccagcg tactttacgt aatgacaccc
     1321 catgcctttg cgcaaggaac ggtgactatt tatctgcctg gcgaacaaca gacactttcc
     1381 gttggcccca tggaaaatgt tgtccagttg gtgacacaac cgcaactgcg cgatcgcctc
     1441 tggtggcctg gtgccttgct gaccgactct gcggcgaaag ctaaagcgct taaagactac
     1501 caacatgtca tggcacagct tgcttcctgg gaagcagaag ctgacgacga tgtggcggca
     1561 acgattaaat ccgtgcgcca gcagttactc aatctcaata tcactggtcg cttaccggtc
     1621 aaactggacc ctgattttgt acgagtggat gagaacagca atcccccgtt ggtcggggac
     1681 tatacgctct ataccgtaca gcgtccggtg actattactc tgctgggtgc ggtttctggg
     1741 gccgggcaat tgccctggca ggcaggccgt agcgttaccg attatctgca agatcacccc
     1801 cgccttgcag gcgcggacaa gaataacgtt atggtgatta cgcctgaggg tgagactgtc
     1861 gtcgcccctg ttgccctgtg gaataaaaga catgtggaac caccgcctgg cagccagctt
     1921 tggttgggct tttctgcgca tgtactgccc gaaaaatatg ctgatttaaa tgaccagatt
     1981 gtgtctgttt taacgcaacg tgttcctgac tgatgaagaa gaattcttat cttttaagct
     2041 gcctggccat tgccgtctcc agtgcctgtc atgctgaagt attaacctac ccggatccgc
     2101 tgggttcgtc gcaatcagac tttggcggca caggattgtt gcagatgcca aatgcgcgca
     2161 tcgcaccgga aggtgaattc agcgtcaact accgggataa cgatcaatac cggttctact
     2221 ccacctccgt ggcgctgttc ccatggctgg aaggcaccat tcgttatacg gatgtgcgca
     2281 cacgcaaata tagccagtgg gaagatttca gcggcgatca gtcatacaaa gacaaatcat
     2341 tcgattttaa acttcgcctg tgggaagaag gttactggct accgcaagtg gcgtttggta
     2401 aacgtgatat tgctggtacg ggtctgtttg acggtgagta tctggtggcc agcaagcaag
     2461 cggggccatt tgatttcacc ctcgggatgg catggggcta cgccggtaat gcgggcaata
     2521 ttaccaaccc gttttgccgg gtgagcgata aatattgtca tcgcgcagag tctcacgatg
     2581 cgggcgatat cagctttagc gatatctttc gtggcccggc ttccatcttt ggcggcattg
     2641 agtatcaaac gccgtggaat cccctgcgtc tgaaactcga atacgatggc aacaattacc
     2701 agaatgattt cgctggcaaa ctgcctcagg caagccattt caacgtcggc gcagtttatc
     2761 gcgctgccag ctgggcagat ctcaacctga gttatgaacg cggtaacacg ttgatgtttg
     2821 gcttcacgtt acggaccaat ttcaacgatc tgcgccctgc cctgcgcgat acgccaaaac
     2881 cggcatatca acctgcgcct gaatctgaag gattgcagta caccacagta gcgaaccaac
     2941 ttaccgcgct gaagtacaac gcaggttttg aagcaccgga aattcagctg cgcgataaga
     3001 cgctgtatat gtctggtcaa caatacaaat accgtgattc tcgcgaagcg gtcgatcgtg
     3061 ccaaccggat tctggtgaat aacctgccgc aaggcgttga gaagattagc gtgacgcaaa
     3121 agcgcgagca tatggcgatg gtgactaccg aaaccgacgt agccagcctg cgcaaacagc
     3181 tggctggtac agcgcctggt caatcagagc aacttcaaca acaacgtgtt gaagcagaag
     3241 atctttctgc ctttggtcgg ggctaccgta ttcgtgaaga tcgctttagc tactctttca
     3301 acccaacact ttcacagtcg ctgggcggcc cggaagattt ctatatgttc cagctggggc
     3361 tgatgtccag tgcccgctac tggtttaccg accacctgct gcttgatggc ggtattttca
     3421 ccaatattta caacaactac gacaagttta agtcttcgca gttgcccgcg gactctaccc
     3481 tgccccgcgt gcgcacgcat atccgtgatt acgttcgcaa tgacgtttat ctcaacaact
     3541 tgcaggcgaa ctactttgcc gacttaggca atggtttcta tggccaggtg tatggcggtt
     3601 atctggaaac gatgtacgcc ggtgtcggtt ccgagctgct ttatcgcccg ctagatgcca
     3661 gctgggcgct gggtgtggac gttaactacg tgaagcaacg tgactgggac aacatgatgc
     3721 gcttcaccga ttattccacg ccaactggtt tcgtgacggc ttactggaac ccgccgacgc
     3781 tcaatggcgt actgatgaaa cttagcgttg ggcaatatct ggcaaaagat aaaggggcaa
     3841 cgatcgacgt cgccaaacgc tttgacagcg gcgtggcggt aggggtatgg gcggcaatca
     3901 gtaacgtatc taaagatgac tacggcgaag gcggctttag taaaggtttt tatatctcga
     3961 ttccgttcga cttgatgacc attggaccta accgcaaccg cgcggtggtt tcgtggacac
     4021 cattgacgcg tgatggtgga caaatgctgt cacgcaaata ccagctctat ccaatgacgg
     4081 cagagcgaga agtaccggtt ggacaataac ctgacgtaat tcataacatt acattgactg
     4141 ataaatataa accaatgaaa aagaacattt ttaagttttc ggtactgaca ttggctgttc
     4201 tgtctttgac agcatgtacc cttgttcctg gtcagaattt atcgaccagt aacaaggatg
     4261 tcattgaact acctgacaat cagtatgacc tggacaaaat ggtcaatatc tatcccgtca
     4321 cgccaggatt gattgatcag ttgcgcgcca aaccgattat gtcgcaggct aacccggagc
     4381 tggagcagca aatcgcaaac tatgaatatc gtatcgggat tggtgatgtg ttgatggtta
     4441 cggtctggga tcacccggaa ctaaccaccc ccgcagggca ataccgtagc gccagtgata
     4501 ccggcaactg ggttaacgcc gatggtgcga ttttctaccc ctatattggt cgcctgaaag
     4561 tggccgggaa aacacttacc caggtacgca acgagattac agcgcgtctc gattcagtca
     4621 ttgaaagccc gcaagtcgat gtcagcgtcg cggcgttccg atcgcaaaaa gcgtacgtca
     4681 ccggcgaagt cagcaaatcg ggtcagcagc cgattaccaa tattcctctg accatcatgg
     4741 atgcgattaa cgccgcgggc ggtctgaccg cagatgcaga ctggcgcaac gtggtgctca
     4801 cccagaatgg cgtaaagacc aaagtgaacc tctatgcgct gatgcagcgt ggcgacttgc
     4861 gccagaacaa actgctgcac ccgggcgata ttttatttat tccgcgcaac gatgatctga
     4921 aagtcttcgt gatgggtgaa gtgggtaaac agagcacgct gaaaatggac cgcagcggta
     4981 tgaccctggc ggaagcgctg ggtaacgctg agggtatgaa tcaggatgtc gctgatgcga
     5041 cggggatttt tgttattcgt gcaacgcaaa acaagcagaa tggcaaaatc gccaatattt
     5101 atcagctgaa tgcgaaggac gcatcggcaa tgatactcgg aacggaattc cagcttgaac
     5161 cttacgacat tgtttatgtc actaccgcgc cgctggctcg ttggaaccgt gtgatttctc
     5221 tgctggtacc gacgatttcc ggggtgcatg acctgacaga gacttcacgg tggattcaaa
     5281 catggcccaa ctaaaattta actcaatcct ggtggtttgt accggcaaca tttgccgttc
     5341 gcccattggc gagcgcttgc tgcgtaaacg tctgccgggc gtaaaagtca aatcggcagg
     5401 tgttcatggt ctgataaaac accctgcaga tgcgacagcg gcagatgttg ccgctaatca
     5461 tggcgtatct cttgaaggac atgccggacg taagctcact gctgagatgg cacgaaatta
     5521 cgatctgatt ctggcaatgg agtcggaaca tattgctcag gttaccgcaa ttgctcccga
     5581 ggtccgcggg aagacaatgc tgtttgggca atggctggaa cagaaagaga tcccggatcc
     5641 ctatcgtaaa agtcaggacg catttgaaca tgtctacggt atgttggagc gcgccagtca
     5701 ggaatgggcg aagcgcctca gccgataatt tgagtttata aaatacgatg acaactaaaa
     5761 atatgaatac gccaccaggc agcactcagg aaaatgagat cgatctgctt cgtctggtcg
     5821 gcgagttatg ggatcaccgt aagtttatta tcagcgtgac cgcgttattc acgctgatcg
     5881 ctgtcgctta ctcgctgtta agcacaccaa tttatcaggc agatactctg gtccaggttg
     5941 agcaaaaaca gggcaacgcc attctcagcg gcctgagcga tatgatccct aactcatcgc
     6001 ccgagtctgc accggagatc caactgctgc aatcgcgcat gattctcggt aaaaccattg
     6061 ctgaactgaa tctgcgcgac atagttgagc agaagtattt tccgattgtg ggtcgcggct
     6121 gggcgagatt aaccaaagaa aaaccaggtg agctggcgat cagctggatg catattccac
     6181 aactgaatgg tcaggatcag caactgacac tcacggttgg ggaaaacggc cactatacac
     6241 tggaaggtga agagttcacc gtcaatggta tggtcggcca gcgtctggaa aaagatggcg
     6301 ttgcgctgac tatcgcggac attaaggcca aaccaggaac acagtttgtc ctgagccagc
     6361 gtaccgaact ggaagcgatt aacgcattgc aggaaacctt taccgttagc gaacgcagta
     6421 aagaaagcgg gatgctggaa cttaccatga ctggtgatga tccccagttg attactcgta
     6481 ttctgaacag catcgctaac aactatttgc aacagaatat cgctcgccag gcggcgcagg
     6541 attcacaaag ccttgaattc ttacagcgcc agttacctga agtgcgcagc gagctggacc
     6601 aggcggaaga aaaactcaac gtttatcgcc agcagcgcga ttcggttgac cttaacctgg
     6661 aagccaaagc cgttcttgag cagattgtga acgttgataa tcaactcaat gagctgacct
     6721 tccgcgaggc agagatctcc cagctgtata agaaagatca cccaacttat cgtgcgctgc
     6781 tggaaaaacg ccagacgctg gagcaagaac gcaaacgcct gaataagcgg gtatcggcaa
     6841 tgccttccac ccaacaggaa gtgttgcgtt taagtcgtga cgtagaagcg ggccgtgcgg
     6901 tatatctgca attacttaac cgccagcagg agttgagtat ttcgaaatcc agtgccattg
     6961 gtaacgtgcg gattatcgac ccggcagtca ctcagccgca gccagtgaaa ccgaaaaaag
     7021 cgttgaatgt ggtgcttggt tttattcttg gcctgtttat ttctgtgggt gccgtgctgg
     7081 cgcgtgcgat gttgcgtcgt ggtgtagaag ccccggaaca actggaagag cacggcatca
     7141 gcgtttatgc cactatccca atgtccgagt ggctggataa acgcacccgt ctgcgtaaga
     7201 aaaatttatt ttctaatcag cagcgccatc gtactaaaaa tatccccttc ctggcggtgg
     7261 ataacccggc ggattctgct gtggaagccg tacgtgcgct acgaaccagt ctgcatttcg
     7321 ctatgatgga gacggagaat aacattctga tgatcaccgg tgcgacgcca gacagtggta
     7381 aaacgtttgt cagttcaact ctggcagcgg tgatcgccca gtccgatcaa aaagtgttat
     7441 ttattgatgc cgacttacgc cgtggttatt cgcataacct gtttaccgtg agtaatgaac
     7501 atggcttgtc ggaatatctg gcaggtaaag atgagctcaa caaagtgatc cagcattttg
     7561 gcaaaggagg ctttgatgtg attactcgcg gtcaggtgcc acctaacccg tctgaactgc
     7621 tgatgcgcga tcggatgcgt caattactgg aatgggcgaa cgaccattac gatctggtga
     7681 ttgtcgatac gccgccgatg ctggcggtga gtgatgccgc ggtcgtgggg cgttctgttg
     7741 gcaccagcct gctggttgcg cgttttggct tgaacaccgc caaagaggtg agtttgtcaa
     7801 tgcagcgtct ggaacaggca ggcgtcaata ttaaaggcgc tatcctcaat ggtgtgatta
     7861 aacgcgccag caccgcttac agttacggct ataactatta cggttatagt tactccgaga
     7921 aagagtaaaa ctgaaagtgg ccggatgcgg cgtgaacgcc ttttccggtc tacgcattag
     7981 acagttcttc gtttgtcatc agcctcagag cattcaggta actgaatgct cttttttatg
     8041 cattacaaac tgcacgccgg tatgcgtgct tcattcacga tttgcgtaaa acctgccaac
     8101 gaacacatgc cctgcgcatt tcgctcttca catcctgcca gggtcagttt cacctctccg
     8161 ggcggcgtat ttaatgacag cggcgtttta tcacgcatct gctgtaaagt ctggaagacc
     8221 agcgaaacct gaatccactg gctgttatcg cttagccgac gccagcgttc aaacaccagt
     8281 tcaccacctg gcggcgtgtt atccggctga ccgggaagcg tccagttgag ctccagtgcg
     8341 ccgccgagat ttgccagatt agtatcgtgt ccggcgataa acagcactga agtgggtaat
     8401 gtcacaccat acgcctgttt ttgcggtgga tggggcgtca acgctgtctt gatcaaatct
     8461 aataacgggg tggcgcggct gcgggcaacc tctggcgtgc gttgtagcaa ataaaattgc
     8521 gcgttatgca aacttagcaa ggtgttccac tggtgtgaat cggtgatcct tccccacccc
     8581 ggctccggca ttccctgtgc ttgttgcagg agaaatatct ccgtcagcat tgatgcgagg
     8641 cttaccgcac cggttaatga gacattgtcg gcgctcacct tgagttccga tggtaatgcc
     8701 tgcgttaatg aacagctttc gtcctgtttc tcacgtttaa ggcacaagtt tgattgcgga
     8761 aaattaagca cccgttccag ttcgcgaaac gccgtttgcc gatgcccggt aaagtcagca
     8821 attgaccctc ctgccctgct gaggatcgcg tcagtcacgt tcgcgttatc cagttggcaa
     8881 acgccagttt ttagaggatt aaataacgga tcgggactgg acgtatcagc ctgggtatgt
     8941 acggttattg cacagtcagg tgccagcccg gcggcgaagg cttcgcctgt tttacgggta
     9001 cgctcgtcga catcagcaat aatcgcgacc tgaccagact gcgggcagcc ctttttcgcc
     9061 agcaatccgt cggctaccag acgctggcgt tggtaatgtc cgagataggc gattagctca
     9121 ccaccgcgcg gtgtcagcca acccagtttt accggccagg ttggccatgc gtctggggtg
     9181 acatcctgca tcagttgcgt ggccttggtt ggagcacgca caccatgacg actgacaatc
     9241 accacacttt ccagcttcag ctccggctca ctctgagcga atgcagattg cggggttaac
     9301 ggaatcagaa gagataaaaa tgggattaag atcgctttca tcgatatgtt tccgcttttc
     9361 atatctgaca ttattgattg cctgatgcga tgctaatgcg ccgcatcagg cctacgttcg
     9421 ttaacttttc agacgcgggt ccagccatac caacacaagg gtggagagcg aacacatcaa
     9481 caaaatgccg acgaaccaaa gtaaatacca cattgttcct gctccttagt acaactcgtt
     9541 ttcgttacgg cggagagttt ctgttgtcat gcgtccccac attttgtagt agctccagag
     9601 agtgtagagc aacacaatgg gcaaaaatat cagcacgatt accaacataa tgctcagcgt
     9661 cagctgactg gaagtgctgt cccacaacgt caggctggag attggactca cgcttgacgg
     9721 catgacaaag gggaacagcg tgatgcctgc cgtgaaaatc acgccaaatt gcatcaatga
     9781 cgccattaaa aatccccaac ccgggcgacc acgataaatc gccatcaccg tcagcaatgg
     9841 gcagaagaac cccagcagcg ggaagatcca caaaacgggc gattcgacga aattattcat
     9901 ccaggcacca ggtagcactg ccaccagttt cattaacgga ttggaaggac cgttagcatc
     9961 ctgggcgagc agtacaaagc catcaatacc gacccacagc cagtaacccg ccagcaaaaa
    10021 gcacagcatc accaacagtg cagcgcgttt ggtggccagc tgtgaacgca gatgaatcac
    10081 accaacagtt ttcagttgta accagacgcc accttgcaaa atcaccatcc caaggctgag
    10141 caatccgcac aataaaggga atggcgtcag cagttgccag aagctaccga gatactccac
    10201 gcgtaattgc ggtgtgaagg caaacggcac gccgagcaac aagttgccga aggcgatacc
    10261 gaagactacc ggcggcacca gactgccgat gaccagaccg gcgtcccaca ttttacgcca
    10321 gcgtgcatcg gcgatttttc cgcgataatc aaaggccagc gggcggaaga acagtgagca
    10381 cagcaccagg atcatcgcca cataaaagcc ggaaaacgcc gctgcataca ctctgggcca
    10441 ggcggcaaat aatgccccac cagcgaggat caaccagacc tggttgcctt cccagtgtgc
    10501 accaacgctg tttatcacta tccggcgttc atcatcatta cgcgccacca gcggcagcag
    10561 acagccgatc cccatgtcaa atccgtcgga gatcataaag accaccagga tcacgccaat
    10621 cagcagccac cagatgaagc gcaatgtttc ataatcaaac atgattttcc cctttacccc
    10681 tgttgctgcg tcggttgttc actctgcatc gcgctcggcc ccagacgggc atatttctgc
    10741 atcaggtaga cttcggcgat taagaacagg gtgtaaagcc ctacgatcat gatcagtgag
    10801 aaagccagtt gtcctgtggt taaagcggag tgcgcggagt atgtcggtaa gatgtcctgt
    10861 atcgcccacg gctgacgacc aaactcggtc ataaaccacc cggcttcaat cgcaatccac
    10921 ggcaacggca aactccagag cgccattttc agcacccagc gatgctggtc gattttgcca
    10981 cgcagcgtct ggacaagcgc aatcagcatc accagtagcg gcagggaacc acagcccacc
    11041 atgatgcgga aactccagaa taccggcgca acctgaggta tcgcgccacg catcgccgcc
    11101 tggtactgtg cggctgtgac atgattcata tccggcgcat agcgggagag caacatgccg
    11161 tagcccaggt caccttccag ttcccggaat gcctgcaaca catgcggctc acgattgcct
    11221 tgcgatattt cctgcattaa cagccaggcc atacgtccgc gttgcaagcg tgggtaggtt
    11281 tcagccatca aatttttcag acccggcacg gttttatcta atgagtgagt ggcgaggatc
    11341 cctaacagcg cgggaatttt gatggcaaag gcgttacgct cttgatcctg ttccggccag
    11401 gcaaccacat ggaacggtgc aggagcaggt tccgtttgcc actccccttc catcgccgcc
    11461 agttttaccg gttgtacttg cgcgacttca tacgcagaac tgtctccgag ttgtagcgtg
    11521 ccgataatcg ccagagtacc gaagacggaa ccgatggcaa acgagcgtaa tgcaacatcg
    11581 cgctcccgtc cgcgcagtaa ataccaggcg ctgatcgcca taataaacat ggccccggtc
    11641 acgtagcccg ccattacggt gtgcacaaat ttcacctggc tgaccgggtt aaagaccagc
    11701 tcgctgaagc tggtcatctc catacgtagg gtgtcgatat caaaatgcgc accggtcggg
    11761 tattgcatcc aaccgttggc attcaatatc cacaatgcag agagatttga accgaacgcc
    11821 accagccacg tcaccagcag gtgctggtat ttattcagcc gctgccagcc gaagaagaaa
    11881 agcccgacaa aggtggattc gaggaagaag gccattaatg cttccatcgc cagcggtgcg
    11941 ccaaaaatat cgcccacata gttggaatag aatgaccagt tagtaccaaa ctgaaactcc
    12001 atggtcaggc cggtagccac gccaagagca aagttgatac cgaagagctt accccagaag
    12061 cgcgtcatat cgcggtagat tgttttgccg gtgaccacgt aaatggtttc catgatagcc
    12121 agcaaaaaaa tcagccccag ggtaaggggt acaaataaaa agtgatacag cgcggtcaga
    12181 gcaaactgcc agcgcgataa atcaatgaca tcccacatgc gcactcctgt aggcgaagag
    12241 agaaaaataa cccgataatg ttagatctcc ctctttatcc tgaaactcta cgaccagagg
    12301 tattcgccga ttgcgatatt cataagactg aaagcgatac ttaccgtctt tttacgtcgg
    12361 tgcagcttcc gccagccact gacatacctc gctaagccgc tgcgcagagt cgaccaaatc
    12421 ttctggcgct gccagcacca cttccggtat tgggcagatt tcataacttt ccagtaaagg
    12481 accttttaag gtgtccgtac agcgtaaatg ccagacatgg cgtaaccccg tggcgttgat
    12541 atagctctcg ccatagccaa tggtacgaat ctgaatattt cccggcccac agagacgtga
    12601 gagaaaaagc cgatcagcct cgctgatggg taattgcgtc agattaatgc tgtgcggctg
    12661 cgcgtcgggg ttacgtacat gcgccagtaa ctcatgcgcc aacggtaggc cattcatcag
    12721 gccatcaatg ggcggcggta acagcgaatc tgtcggcaag agattttgcg tggccgcctg
    12781 ccacaacgcc agcggcgcgc agccggcctc cagtttgtcc tccagcaact tttcgccgcg
    12841 acgtttgcgc acccgccata atccgcagaa gatcgcctcc tgtatttcac tttcgctgtc
    12901 gtcagcctgc tgaatgcgca ctgacacttc cccttccccg agaagcgtat tgataaagtg
    12961 acgatcgtcg gcgttgagat ttaacagatc gactgccagc acctcgccca acggcggctg
    13021 gcgttgactt agttgatgtt gtaactcgtt taacagcgca atcacctgcg ggctgtgagc
    13081 acattgctcc agggccgcca tactcggttc atcattcacc tgacagctga tcggcagtgg
    13141 attcatgctg aaactgtcat cgttcggttg cgttcctggc cccagcagat ggaaaaaagt
    13201 ttcgctcatg aggcacgctc ctgttgtggt tcgacaagcc cacgcatcag gtttatcagt
    13261 tccgctcacg ggtgaatacc attcagcaca ccgcgatagt ttccgccggt aaacactaat
    13321 gtggcgggaa agcgaaagac gccaaagcga tctccgatgg cttcgctctg ttcaaggtca
    13381 gcaatcgcca cctgccatgt gtagccggga aactcgcgca gtaattcgcc aatcattacc
    13441 ggattatcgc tgacctctgg cgtgcgtttc gggtcactgc ttaataacac cacgccgtct
    13501 ggcgcttgcg taagccagtc gtcaagacgg aattcactaa ccggcgtcca gccgcgcgcc
    13561 agcattcgtt gccacaacgc atcaaatggc gtgtcgttgc tcatccctgc tcctccagtg
    13621 tcatcctgta ctggcgcaag cgaacgcctt cgtaattttc catcgacaga cagtcataat
    13681 tgagacagcg cgattcatta gccggttgcg gcacaattcc ccatgcagcc agctgcgcca
    13741 gcgccgcctg ttccgctgcg ggcagttgct cccgtgccag ttcgctcagg cggccgccgt
    13801 agtcgtcgag cattgcgggt tgcagaccga cgagggcaat atgtgctggc agatgtccgc
    13861 ggatatctgc cagcgccagc acttcggaga aactgttctg gtgcaggctc attttcttcg
    13921 cgctgagata agccggaata cgttctccgg cataggttcg cagcgttcca ggttccagcc
    13981 cgtagtcaat ggcatcgaga atcaacagat ggctggcgct ttcgacatac cccagcaagt
    14041 tcagtccctg agtaccgcca tcgacaatct ccacatactc gggccagtgg taatgggcat
    14101 acaaccgttc cgccacccgc acgccgaaac cttcatcggc ccacagcagg ttgcccagcc
    14161 ccattaccac aacgcgttgc tcgctcatga acgctccttg ttacttattt tgccaaattt
    14221 gtggctacgg tagccgttga ccatggtgga gatcaccgtg tcgtcggaca tgatgtcttc
    14281 acgcagcgcc atatagacat gaccgatcac aaacgcgcca atcagccaca tccccagccg
    14341 atgccagctg tgaatgtcca ttgagttgcc tcccgtccag tagaaaaatt ccaccacata
    14401 acggaacggc gcaaaaatag cgtactggct gtgttcgctg tacagcgcaa agccggtgat
    14461 gatcataaag accgacatca ggaaatagcc gaacatcgcc gcctgggcga tggggttatg
    14521 gcctatatcg gcactcggac gttttgccag aacagatacc agcggatttc ataccacacg
    14581 ccctgccacc agcttttacg ccataccggc acgataaaca gctcgcggga gtatcgattg
    14641 ccaacaaaag cccagtagat ccgcatcagc aagaccacgg taaaaaccat cccggcgctg
    14701 aagtgaatta acctgatgta gcccatatag aacagatacg tcgcctcgcc gctgacggaa
    14761 ggtagcggct tgccgataaa gtatccggtg accatcaaca ccgccatgca taacaccgtc
    14821 agccagtgcc agatgcggac tggcgcttca aagacatagt ggctgacaac gttgtcgctt
    14881 ttctgttgca tgatgattct ccttcgctgt taacgcacct gcacggagat cagctcgcta
    14941 ccgtcgtcgc ccagcacgtg tgttgaacag gcgaggcacg ggtcaaagct gtgcagagta
    15001 cgcaggatct ccagcggttg ctcggggatc gccattttgg tgttcatcag cgccgcttca
    15061 taagcgccaa tctgcccttt gggatcgcgc gggctggcgt tccaggtggt cggcaccacg
    15121 cactggtaga gatcaatctt gccatcgcga atggcggccc agtggcctaa cgccccgcgc
    15181 ggcgcttcgg taaagccgac gccacggcac tctgtcggcc aggttgcagg ttcccatttt
    15241 tccgtggagg cggtggcaag attgccgttt ttcaggttag tcatcagctt gttgaagaaa
    15301 tattgcaact tacctgcggc ccactgtgct tcgtgcgcgc ggcacaaaat gcggcccaac
    15361 gttgactgga taccggaaag cggcaggttc agcgccgaca tcatgcgatc gaccgactca
    15421 acggtcgcag catcgccttt gtgataagcg attaacgtgc gtgccagcgg ccccacttcc
    15481 atcgcgttac cgcgccagcg cggcgctttg atccacgagt agcgttcctg ttcattcagc
    15541 tgctgaatgt tggtatcgct gcctttgaca tcgccggggt tgtaccacgg gtcggtaatg
    15601 ccatcgaatg gatgacgccc gacctgatcg ttgggataac gataccaggc gtggtcgaca
    15661 aactcctgca cctgctgcgg atcaaccaaa tccactggca gcacattgtt gaagtcgccg
    15721 ttaatcaccg cgccgccagg catcagcaga cttttctcac caaagtcgtt ggcaatatcc
    15781 gggaacgcac cgtagctgag gacacattta tcagaaagac ccgtgccgat ttcgctccac
    15841 ggtttgttga actgaccgat ggctaaggcg tcggggatca tcacgttgtt aatgaagtcc
    15901 gccgtgcggg tgataatcga ctgcaccagg ttcaggcgtt ccatattgac cgccccgacc
    15961 gcgccgcttt cgtcaatgtt aatggcgcaa ggcatcccgc cgacaatcca gtttggatgc
    16021 gggtttttac cgccaaagac cgcgtggatt ttgacaattt cacgctggaa atcgagagct
    16081 tcgagatagt gggcaaagcc catcaggtta gcttctggcg gcagtttgta ctgcgggtgc
    16141 ccccagtagc cattgcggaa gatccccaac tgcccgcctt caacaaattt tttcaggcgg
    16201 ttttgtacgt cgaagaaata gccaggggat gatttcggcc aagaggagag actttgcgcc
    16261 agttcggagg ttttccgcgg gtcggctttc agcgcatcta acacatcgat ccagtccatc
    16321 ccggcaagct gatagaagtg caccagatga tcgtggcacc agagcgttgc cagcataatg
    16381 ttgcggatga tattggcgtt gtccggcact ttaataccga tagcatcttc gatggcgtaa
    16441 accgaagcca gggcgtgtac gccagtacag acgccgcaga tacgttcaac gaacgcccac
    16501 gcatcgcgcg ggtcgcgccc ttgtaggatg atctccagcc cgcgaaacat ggtgccgcag
    16561 gagacggcat tggtgatcac attctgatcg ttaatattca cttcgcagcg catgtggcct
    16621 tcgatgcgcg taatcgggtc gaccaccagg cggcgtccgg cattattgat ggtgtatccc
    16681 tgagtttcgt actgagtgct catgcctgtt tatcctcatt gcctggctga tgttcggttt
    16741 ctgtaggttg ctggttatga cgtctgcgct ggtcaacggc gctggcgact gcgtgcacac
    16801 caacagccgc tgccaccaca ccaagcgcgg ttaaaccgac ggtatcggcg gtggaatgag
    16861 tacccatttg cggaatatcg accacgcggc tgtagaacga accgcaatcc cagaaaccat
    16921 tttccgcaca gcccaggcag ccgtgaccag actggattgg gaaagaaacg ccatcattcc
    16981 agcgtgtgga ggaacaggcg ttataggtgg taggcccttt gcagcccatt ttgtacaggc
    17041 agtaaccttt gcgggcagcg tcatcatccc aactctggac gaactctccg gcgtcgaagt
    17101 gggcgcggcg atagcattta tcgtggattc gctgaccata gaacatcagc ggacggccca
    17161 ttctgtcgac atctggcaag cgatcaaagg tcaccatgta agtaatgatg gcgctcatca
    17221 catccgggat cggcgggcag ccaggtactt tgataatggg tttgtcggtg atgactttgt
    17281 cgataggcgt tgcctgcgtc ggattgggcc acgcggcctg cacgcagccc caggacgcgc
    17341 aggttcccca ggcgataatc gcgctggctc cggcagcggc acgtttgagt ttctcaataa
    17401 acggtcgacc gctgctgata cagaacatcc cctgctcgcc cagcggcgga ttaccttcta
    17461 ctgcgaggat atatttgcca ttgtattgcg tgatgatgtc ttcgaagact tcttccgcct
    17521 gggttccggc ggcagccatc aaagtatcgt cataatcgag ggaaatcagg gaaaggatga
    17581 cgtccttcgc cagtgggtga gcggagcgga taaaagattc ggtacagcag gtgcattcca
    17641 gaccgtggat ccataccacc ggaatgcggg gtttgttctc cagcgcccag gcaatctttg
    17701 gtgccattcc cgcgcctaat cccagcgacg tggcagccag actacaatat ttgagaaagc
    17761 tgcgccgggt aacgccctga cgccgcatgg cctggtaaaa tgtttcctcg ttattcatat
    17821 cgcacgtctc ttctccttgc gacaccggca ggacaccggg cgcttatggt cggcaagcga
    17881 cgtccctgta gcaaggcaaa ccatcgcttc acgtgagatg ctgaaaacga aagctcatcc
    17941 ttctgcactg gcgcacgtcg ccagaaagta ttgttaataa agcgtagtga aact
//
LOCUS       DAZRUF010000070        17919 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZRUF010000070 DAZRUF010000000
VERSION     DAZRUF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.070"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..513
                     /locus_tag="ACHXOL_001889"
     CDS             <1..513
                     /locus_tag="ACHXOL_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR6808880.1"
                     /translation="AAITCISQPKKTTLRDKAMVNYAFDYLRSPGSLPLTTAATELSA
                     IHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGV
                     KPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYKNA
                     ERLLAGLPAH"
     gene            complement(523..1950)
                     /locus_tag="ACHXOL_001890"
     CDS             complement(523..1950)
                     /locus_tag="ACHXOL_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR6808881.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1947..2654)
                     /locus_tag="ACHXOL_001891"
     CDS             complement(1947..2654)
                     /locus_tag="ACHXOL_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR6808882.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            2854..3795
                     /gene="ybeQ"
                     /locus_tag="ACHXOL_001892"
     CDS             2854..3795
                     /gene="ybeQ"
                     /locus_tag="ACHXOL_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR6808883.1"
                     /translation="MDEIIERAEKGDCEAQYIVGFYYNRDSAIDSPDDEKAFYWLKLA
                     AEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASNALGWTLDRGEAP
                     NYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYKQAALQGHSDAQN
                     NLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGEGVDQDYKQAMYW
                     YLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDATAWYNLAIMYHY
                     GEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3865..4347)
                     /locus_tag="ACHXOL_001893"
     CDS             complement(3865..4347)
                     /locus_tag="ACHXOL_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR6808884.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4582..7164
                     /gene="leuS"
                     /locus_tag="ACHXOL_001894"
     CDS             4582..7164
                     /gene="leuS"
                     /locus_tag="ACHXOL_001894"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR6808885.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7179..7760
                     /gene="lptE"
                     /locus_tag="ACHXOL_001895"
     CDS             7179..7760
                     /gene="lptE"
                     /locus_tag="ACHXOL_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR6808886.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7760..8791
                     /gene="holA"
                     /locus_tag="ACHXOL_001896"
     CDS             7760..8791
                     /gene="holA"
                     /locus_tag="ACHXOL_001896"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR6808887.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            8793..9434
                     /gene="nadD"
                     /locus_tag="ACHXOL_001897"
     CDS             8793..9434
                     /gene="nadD"
                     /locus_tag="ACHXOL_001897"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR6808888.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9458..10069
                     /locus_tag="ACHXOL_001898"
                     /pseudo
     CDS             9458..10069
                     /locus_tag="ACHXOL_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            10329..10646
                     /gene="rsfS"
                     /locus_tag="ACHXOL_001899"
     CDS             10329..10646
                     /gene="rsfS"
                     /locus_tag="ACHXOL_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR6808889.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10650..11117
                     /gene="rlmH"
                     /locus_tag="ACHXOL_001900"
     CDS             10650..11117
                     /gene="rlmH"
                     /locus_tag="ACHXOL_001900"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR6808890.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11148..13049
                     /gene="mrdA"
                     /locus_tag="ACHXOL_001901"
     CDS             11148..13049
                     /gene="mrdA"
                     /locus_tag="ACHXOL_001901"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR6808891.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13052..14164
                     /gene="mrdB"
                     /locus_tag="ACHXOL_001902"
                     /gene_synonym="rodA"
     CDS             13052..14164
                     /gene="mrdB"
                     /locus_tag="ACHXOL_001902"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR6808892.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14175..15263
                     /gene="rlpA"
                     /locus_tag="ACHXOL_001903"
     CDS             14175..15263
                     /gene="rlpA"
                     /locus_tag="ACHXOL_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR6808893.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15402..16613
                     /gene="dacA"
                     /locus_tag="ACHXOL_001904"
     CDS             15402..16613
                     /gene="dacA"
                     /locus_tag="ACHXOL_001904"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR6808894.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16723..16986
                     /gene="ybeD"
                     /locus_tag="ACHXOL_001905"
     CDS             16723..16986
                     /gene="ybeD"
                     /locus_tag="ACHXOL_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR6808895.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17087..17728
                     /gene="lipB"
                     /locus_tag="ACHXOL_001906"
     CDS             17087..17728
                     /gene="lipB"
                     /locus_tag="ACHXOL_001906"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR6808896.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4537 a         4510 c         4569 g         4303 t
ORIGIN      
        1 gcagcaatta cctgtattag ccaacccaag aaaacgacgt tgcgtgataa agccatggtg
       61 aattatgcct ttgattattt aagatcacca ggcagtctac cgttaaccac ggcagccacg
      121 gagctttccg cgattcatgg tcactcaaca tcgcaatatc gccttggcga attttatctt
      181 catggtagcg acggtaaacc actggattat acacaggcga gatactggta tgagcaatca
      241 gcagaacagg aaaatccgcg cgcacaaagc aaactggggt ggatctacct gaaaggcctg
      301 ggagtcaaac ccgatacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg
      361 tatgctcatg ctcaatatac tttgggtttg atctacagaa atggcactgg tattaatgtt
      421 aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg
      481 gaaagattac ttgccggact tcccgcacat taacgtcgac tgtcacccga agagatggag
      541 tatttttcct atcaaaccgg caaagataaa aatgtagaaa ataattttga tgacgcccag
      601 acagcccagc ccgccatcgt caacgggcat tggctgcggt tgcggctccg gctcgacggt
      661 ttcctgaccg gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag
      721 tggacaacga tcttctgcat caaggcggct cacaatcggc attaatgatt cttctgtcgg
      781 ttggctggcc tgcactaatt gtttggcaac taaatataat ggccataagg gatgttctgt
      841 atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct cgaccagtga
      901 acgccccagc gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg
      961 ggcgctgtcg ccggggacat aaagtagctg tttaccctga tcgttaacca gttcacgagc
     1021 ttcaatcatc atcaccacta acgggtaata atcgacccga tgctgacggc accattgcaa
     1081 tattaaagca tccgcctggg tggagggata ctcgcgccag tagtcacaaa gttctgccag
     1141 caagctttcg ccttctccac aatagacgcg ttgcgcgtat tcataataac gcgcatcttc
     1201 attatcaggt tcagcttccc gccacgaccg ggcgtaatcc agaagttcgg ggatatccag
     1261 gcgacctgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggatgat
     1321 cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg
     1381 agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaacgggca gatgcagaat
     1441 ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt ccagttcttc
     1501 ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac
     1561 cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg accagcgtaa
     1621 ttcatcaatc tcttccatcg agcaataatt taattgctga atgaatcgct gccaggcgga
     1681 gggcagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca gaatttcatg
     1741 ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg
     1801 gagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg
     1861 aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag tcgtttcctc
     1921 tatttcgagg atcttccagc aatttttcat aggtcgctca tatccttaag cgtttccggg
     1981 catgtggggt agtcagccag tggttgccag gacaaggcag ggtaatacgc atcatcactg
     2041 gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg agctaagaat
     2101 tcgcgtaata aaggcagatg atcatcttcg ggagactgcc aatattgccg accaaaggta
     2161 taaccggcaa agtattgccg ccagttacta taataacgta gagcccgtag atgaatacgc
     2221 gactggatcc atagactttc ttcttcactc aaccagttat taagtacgcc cattcgactt
     2281 aaaaaaccca tccggacata atcccatgct ttaattcctc cctcaccaca gcattcggct
     2341 gtgctggcga caaattgcgc ataagcctga ccttgctcat ttagttcagc aagatagtca
     2401 cgccattcac atggcgaata acgaaaccag cggtgataaa accctgccaa ccgagcggca
     2461 tgaccgttat cgatcattcg gtgaattgtg cgcagcaggt catcacggtc atcaattccc
     2521 catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttggggga tgaatgcgaa
     2581 ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattttttgc
     2641 gaccccatgt ccatataatt tatccttaaa cattcattta atgaatgtaa actacatttc
     2701 gagactcagt tatttatact tttctgacgc aacataatac gcataaaata aggcttacgc
     2761 aaaaatggaa cacataaaaa tataatatgt tttatctaca accataagga aatgataatg
     2821 gtttttacgt caagttgctg cgataattta taaatagatg agattatcga acgtgctgaa
     2881 aaaggcgatt gcgaggctca atatattgtt gggttttatt ataatcgcga tagcgcaatt
     2941 gactctccgg acgacgaaaa ggccttttac tggctgaagc tggccgctga gcaaggtcat
     3001 tgtgaagcac agtattcctt agggcaaaag tataccgagg ataaaagccg tcataaagat
     3061 aatgagcaag ccatcttctg gctgaaaaaa gctgccctac aaggccatac tttcgcttcc
     3121 aacgcccttg gctggacact ggatcgtgga gaagccccca attataaaga agcggttgtc
     3181 tggtatcaga tagccgcgga gagcggaatg tcttatgcgc aaaataatct tgggtggatg
     3241 tacagaaatg gcaacggagt cgcaaaagac tatgcgctgg catttttctg gtacaaacaa
     3301 gctgcattac aaggccatag tgacgcgcaa aacaatctgg ccgatcttta tgaagacgga
     3361 aaaggcgttg ctcaaaacaa gacactcgcc gcattctggt atttgaaaag cgcacagcag
     3421 ggtaatcggc acgcccagtt tcaaattgca tgggattata acgctggcga aggggtggac
     3481 caggactata agcaagcgat gtactggtat ctgaaggctg ccgctcagga aagcgtcggc
     3541 gcttacgtca acatcggtta tatgtataaa cacggacaag gcgttgagaa agattatcag
     3601 gctgcctttg aatggtttac gaaagccgct gaatgcaatg acgccactgc ctggtataac
     3661 ctggccatta tgtatcacta cggagaagga agacctgtcg atctccgaca ggctctcgac
     3721 ctgtatcgta aagttcagtc atccggaacc agagatgtca gtcaggaaat tcgtgagact
     3781 gaagatttac tgtagagata aaaacaaggg ccggggaatt tcattcggcc catattctct
     3841 ttattgctgt gcgaaatcag actattacgg ctcaaacggg cgtctctgga actggtcatg
     3901 accacatttc gggcatagcg tcagcacttc cggtgtgtag atcgggagat ggaagtgaca
     3961 tttctcgcag accagatttc ccagcccgac cacttctccg ctgtgataaa ccccatgatg
     4021 attgaggtcc tggaaaactt cgcgccattc aagctgcgtt ttatcggtga tatctgccag
     4081 ctcctgccat aagctttctt taatcacccg cataaagacg ctgtcagatt cttctttcag
     4141 gctctcttca tagctcatgg cgaactcttc caggtcacgt cttacagctc gcgtcagctc
     4201 atcgacctcg gttcgcgtta actcccctgt ttttattacg cgctcgcgcg cctgttccac
     4261 cagtgcgtcg atatcacgtt cgccattgcg caggcgttcg ctcagtgacg cgaccagttc
     4321 acggtaatat tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa ctgactataa
     4381 taatagacgt tctttgttcc ttgctttgct aatacgccca caaactctgt aaattaatgt
     4441 atgaataatc cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt gacgcggacg
     4501 tttgggctat gctatgcgga tctgaaaaac cacctcaacg ctacatttgt agccgtattg
     4561 aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat agaatccaaa
     4621 gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag
     4681 aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat gggccacgta
     4741 cgtaactaca ccatcggtga cgtgatcgcc cgctaccagc gtatgctggg caaaaacgtc
     4801 ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc ggtgaaaaac
     4861 aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa
     4921 atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc ggaatactac
     4981 cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata taagaagact
     5041 tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca agttatcgac
     5101 ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca gtggtttatc
     5161 aaaatcactg cttacgctga cgagctgctc aacgatctgg ataaactgga tcactggcca
     5221 gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt ggagatcacc
     5281 ttcaacgtta acgactatga caacatgctg accgtttaca ctacccgccc ggacaccttt
     5341 atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa agcggcggaa
     5401 aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt tgccgaagct
     5461 gaaatggcga cgatggagaa aaaaggcgtc gatactggct ttaaagctgt tcacccatta
     5521 acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta cggcacgggc
     5581 gcagttatgg cggttccggg tcacgaccag cgcgactacg agtttgcctc taaatacggc
     5641 ctgaacatca aaccagttat cctggctgct gacggctctg agccagatct ttctcagcaa
     5701 gccctgactg aaaaaggcgt gctgttcaac tctggcgaat tcaacggtct tgaccatgaa
     5761 gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga gcgtaaagtg
     5821 aactaccgcc tgcgcgactg gggtgtttct cgtcagcgtt actggggcgc gccgattccg
     5881 atggtgacgc tggaagacgg taccgtaatg ccgaccccgg acgaccagct gccggtgatc
     5941 ctgccggaag atgtggtgat ggacggcatt accagcccga ttaaagcaga tccggagtgg
     6001 gcaaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt cgacaccttt
     6061 atggagtcct cctggtacta tgcgcgctac acttgcccag agtacaaaga aggtatgctg
     6121 gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg tatcgaacac
     6181 gccattatgc acctgctcta cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg
     6241 gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc
     6301 ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga tgctatcgtt
     6361 gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt
     6421 tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg
     6481 gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc tccggctgat
     6541 atgactctcg aatggcagga atccggtgtg gaaggggcta accgcttcct gaaacgtgtc
     6601 tggaaactgg tttacgagca cacagcaaaa ggtgatgttg cggcactgaa cgttgatgcg
     6661 ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaacgatcgc taaagtgacc
     6721 gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat ggagctgatg
     6781 aacaaactgg cgaaagcacc aaccgatggc gagcaggatc gcgctctgat gcaggaagcg
     6841 ctgctggccg ttgtccgtat gcttaacccg ttcaccccgc acatctgctt cacgctgtgg
     6901 caggaactga aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa
     6961 gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt ccgtgccaaa
     7021 atcaccgttc tggtggacgc aacggaagaa caggttcgcg aacgtgctgg ccaggaacat
     7081 ctggtagcaa aatatcttga tggcgttacc gtgcgtaaag tgatttacgt accaggtaaa
     7141 ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg gcaacattgt
     7201 tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt gataccacgc
     7261 aggttccttc cactatgaag gtcatgatcc tggactcagg cgatccgaac gggccattaa
     7321 gccgtgcagt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat aaagaaacca
     7381 cgcgtaagga cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa gataccgcat
     7441 cggtattccg taacggtcaa acagcagagt atcagatgat catgacggtg aatgcgaccg
     7501 tgttgatccc cggccgtgat atctacccga ttagcgccaa agtcttccgt tcgttctttg
     7561 ataacccgca aatggcgtta gcgaaagata acgaacaaga catgatcgta aaagagatgt
     7621 acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct gcggatattc
     7681 gttccgacga agaacagacg tcgaccacaa cggatactcc ggcaacgcct gcacgcgtct
     7741 ccaccacgct gggtaactga tgattcggtt gtacccggaa caactccgcg cgcagctcaa
     7801 tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat tgcaggaaag
     7861 ccaggacgct gttcgtcagg tagctgcggc acaaggattc gaagaacacc acactttttc
     7921 cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcta tgagtctgtt
     7981 tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaatg cggcgatcaa
     8041 tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctgttga tcgtccgcgg
     8101 taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgctcttg cgaatcgcag
     8161 cgtgcaggtg acctgtcaga caccggagca ggctcagctt ccccgctggg ttgctgcgcg
     8221 cgcaaaacag ctcaacttag aactggatga cgcggcaaat caggtgctct gctactgtta
     8281 tgaaggtaac ctgctggcgc tggctcaggc actggagcgt ttatcgctgc tctggccaga
     8341 cggcaaattg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc atttcacccc
     8401 ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcattgc atattcttca
     8461 gcaactgcgt ctggaaggca gcgaaccggt tattttgttg cgcacattac aacgtgaact
     8521 gttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgtg cgttgtttga
     8581 taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa atcgcttaag
     8641 tcagacgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca ccctcaaaca
     8701 agattacggt cagtcagtgt gggcagagct gaaagggtta tctcttctgt tgtgccataa
     8761 acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct ctgtttggcg
     8821 gcacctttga tccggtgcac tatggtcatc taaaacccgt ggaaacgctg gcgaatttga
     8881 ttggtctgac gcgggtcaca atcatcccta ataatgttcc tccgcatcgt ccccagccgg
     8941 aagcgaacag cgtgcagcgt aaacacatgc ttgaactggc gattgccgac aagccattat
     9001 ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgcg caaacactga
     9061 aagagtggcg gcaggaacaa ggaccggacg tgccgctggc gtttattatt ggtcaggatt
     9121 cactgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat gcacatttga
     9181 tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac cagcaatggc
     9241 tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc ggtaaaattt
     9301 atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa cgtttgcaaa
     9361 acggtgaatc gtgtgaggat ttattgccgg aaccggtact gacttacatt aaccaacaag
     9421 gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt taattcgtca
     9481 tggtgaaacg caagcgaata tcgatggtct ttacagcggt catgcgccca cccccctgac
     9541 cgcgcgcggt attgagcaag cgcaaaatct gcatacgctg ctacatggtg tttcctttga
     9601 tctggtttta tgcagtgaac tggaacgggc acagcatacc gcacgactgg ttctcagtga
     9661 ccgccagctc cccgtgcaaa tcatccctga actcaacgaa atgttttttg gcgactggga
     9721 gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa
     9781 tgactggcag catgcaatcc ccacgaacgg tgaaggattt caggcatttt cgcaacgtgt
     9841 ggaacgcttt atcgcaaggc ttagtgaatt tcagcactat cagaatattt tagtcgtcag
     9901 ccatcagggt gtactgagtc tgttaatcgc ccgtttaatt ggcatgcctg ccgaggctat
     9961 gtggcatttt cgcgttgact aggggtgttg gagcgccatt gatatcaacc aaaaattcgc
    10021 gacgctacgc gtcctcaata gccgtgccat cggggtcgag aatgcatgac ttttctgttt
    10081 ttttacgggt aagccgcaac gaccattgac agacccgggc aggctgatat tctccgcagc
    10141 cagacttttt tcgccagaca cgactttgta gaaattgttt tacaaaaatg gcgatgcaat
    10201 ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga caattgtccc
    10261 gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga ttcacccagg
    10321 gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt gatgacctca
    10381 aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc gactgcatga
    10441 tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac gttgtgcagg
    10501 agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga
    10561 ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt
    10621 atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc gtgggaacga
    10681 aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt ccgaaagata
    10741 tgcccttcga gctgattgaa attccggccg gaaaacgcgg caagaatgcg gacatcaagc
    10801 gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac cgcattgtca
    10861 ccctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag ctggaacgct
    10921 ggaagctgga tggtcgcgac gtcagtctac tgattggcgg gcctgaaggg ttgtcgcctg
    10981 cctgtaaagc ggcggcggag cagagctggt cgctgtcggc gcttaccctc ccccatccgc
    11041 tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc accaaccatc
    11101 cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg aaactacaga
    11161 actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct
    11221 ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat ctgcaaattg
    11281 ttcgctttac cgactaccag actcgctcta atgaaaaccg cattaagctg gtgcctatcg
    11341 cgcccagccg cggcattatc tacgaccgta atggtatccc tctggccctc aaccgcacta
    11401 tctaccagat agaaatgatg ccagagaaag tcgataacgt gcagcaaacg ctggacgctt
    11461 tgcgcagcgt ggttgatctg accgatgacg atattgctgc attccgaaaa gagcgcgcac
    11521 gttcacaccg tttcacctct attccggtga aaactaacct gaccgaagta caagtagctc
    11581 gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat aaacgtcgtt
    11641 actatcctta cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata
    11701 aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca acgcatgata
    11761 tcggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag accggttatg
    11821 aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc
    11881 aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat attgaaacgc
    11941 tgctggcggg tagccgcgca gctgtggttg tcaccgatcc gcgtacaggt ggggtgctgg
    12001 cgctggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc tccagcaaag
    12061 attattccgc cttgttgaac gccccgaata caccgctggt gaaccgcgcc acacaggggg
    12121 tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcattg agcgccgggg
    12181 tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactgcca ggttcggaaa
    12241 aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca agatcgctgg
    12301 aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc gatcgcctct
    12361 ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg gcggaagaac
    12421 gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc
    12481 agggtgacac cattccggtt ggtatcggtc agggttactg gacagcgacc ccaatccaga
    12541 tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttcct catttgctga
    12601 tgagcaccgc cgaagacggc aaacaggtgc catgggtaca gccgcatgaa ccgcccgtcg
    12661 gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt gttgctaacc
    12721 gccctaacgg tacggcgcat aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat
    12781 ccggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg
    12841 ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac aatccgcaag
    12901 tggctgtcgc catgattctg gagaacggtg gtgcgggtcc ggcggttggt acactgatgc
    12961 gccagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct gcggaaaatc
    13021 cagcggttgc cgcagcggag gaccattaat catgacggat aatccgaata aaaaaacatt
    13081 ctgggataaa gtccatctcg atcccacaat gctgctgatc ttactggcat tgctggttta
    13141 cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat
    13201 cggccaaatc gcgatgggtc tagtcatcat ggtggtgatg gcgcaaattc ctccacgcgt
    13261 ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg tggcggtaga
    13321 tgctttcggt gccatctcta aaggtgctca acgctggctg gacctcggta ttgttcgttt
    13381 tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct ttatcaaccg
    13441 cgacgtttgc ccgccatcgt tgaagaacac tggcatcgcg ctggtgctga tatttatgcc
    13501 cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg cgctttccgg
    13561 tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg cagtagtgct
    13621 ggtggcggcg ttcattccga ttctgtggtt cttcctgatg catgattacc agcgccagcg
    13681 cgtaatgatg ctcctagacc cggaatcaga cccactcggc gcgggctatc acattattca
    13741 gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc acggcactca
    13801 gtcacagctt gaatttctcc ccgaacgcca taccgacttt atcttcgcgg tactggcgga
    13861 agagctggga ttagtgggca ttctgattct gctcgctctc tacattctgc tgatcatgcg
    13921 cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg ctggtggctt
    13981 aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg gtattctgcc
    14041 ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa ttgtgctgat
    14101 ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt
    14161 gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca ggaatgctcg
    14221 cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag cctgcggtat
    14281 gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca ctgaacgcga
    14341 cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag gatccgtctc
    14401 gatttagcca ggcgggactg gcggcaatct atgatgccga acccggcagt aacctgacgg
    14461 cctctggcga agctttcgat ccgacacagc tgacggcggc ccatccaacg cttccgatcc
    14521 ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg cgcattaatg
    14581 atcgcggccc ttacggcaac gaccgcgtta tttcactttc tcgcgcggca gctgaccgtc
    14641 ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc caggatggtt
    14701 cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttac gccctgccag
    14761 cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg cagggtgaca
    14821 ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca
    14881 gtagcggttt cctcggcgct ccaacgacct tagcgcctgg tgtactggaa ggcagcgaac
    14941 cgacgcctgc gccacagccc gttgttacag ctccgtcgac aacgcctgca acctcgcctg
    15001 caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg gtgcaagtcg
    15061 gggccgtaag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga cagaagttcg
    15121 gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt ggcccattcg
    15181 ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc caattacagt
    15241 catttattac taccgcgcag tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat
    15301 gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt gctatagtag
    15361 ggcacttttt taattccatc acggatgtcg tagttcagac catgaatacc attttttccg
    15421 ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt atctctgctg
    15481 cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag atcgatgcgg
    15541 agtcctacat cctgattgac tataactccg gcaaagtgct cgccgaacag aacgcagatg
    15601 tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc ggccaggcaa
    15661 tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac gcatgggcca
    15721 ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc atgcaggttc
    15781 cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct tgtgtcgcca
    15841 tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac agctacgtta
    15901 acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat gctgatggtc
    15961 agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt gacgtaccga
    16021 atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc cagctgaacc
    16081 gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc ggacacactg
    16141 acaaagcagg ttacaacctt gttgcttctg cgactgaagg ccagatgcgc ttgatttctg
    16201 cggtgatggg cggacgtact tttaaaggcc gtgaagccga aagtaaaaaa ctgctaacct
    16261 ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag ttcgcctctg
    16321 aaccggtttg gtttggtgat tctgatcgcg cttcgttagg ggttgataaa gacgtgtacc
    16381 tgaccattcc acgtggtcgc atgaaagatc tgaaagccag ctatgtgctg aacagcagtg
    16441 aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc cagcttgatg
    16501 gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa ggtaacttct
    16561 tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt taaaaattga
    16621 acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa ctcccgcctt
    16681 ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac caaacttaac
    16741 gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc gttacctgag
    16801 ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta caccccaacg
    16861 gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa cgccactcat
    16921 atcgagcagg ttgaaacact gtatgaagaa ctaggcaaaa tcgatattgt ccgcatggtt
    16981 ctgtaactcg cttctccgtt acccggcccc tggtcgggta actcccctct cctcgctgtg
    17041 atatactttt cccccacttt tactcattct ccacggagat gcagttttgt atcaggataa
    17101 aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccagg ctatgcatga
    17161 attcaccgat acccgcgatg atagtaccct tgatgaaatc tggctggtcg agcactatcc
    17221 ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg gtgatattcc
    17281 ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccggggc aacaggtgat
    17341 gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg tgaccttgct
    17401 tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc ctcgggctga
    17461 cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac gtattcgacg
    17521 cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac catttttacg
    17581 tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat ggaaacccga
    17641 agcgacgact aataatattg caccacgttt actggaaaat attttagcgc tactaaacaa
    17701 tcctgacttc gaatatatta ccgcttaatt gcatacctca atggcccaat ttacactggg
    17761 tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat actttcttta
    17821 tcacttgcag aaatgcggtg ctacgccttg ctccgtaact caccccattg attaacctat
    17881 tgaaatagtt gattatggtg agcaaaagcg ttccgttcc
//
LOCUS       DAZRUF010000071        17844 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZRUF010000071 DAZRUF010000000
VERSION     DAZRUF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.071"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            128..806
                     /locus_tag="ACHXOL_001907"
                     /pseudo
     CDS             128..806
                     /locus_tag="ACHXOL_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            850..5700
                     /locus_tag="ACHXOL_001908"
                     /pseudo
     CDS             850..5700
                     /locus_tag="ACHXOL_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6068..7723
                     /gene="lldP"
                     /locus_tag="ACHXOL_001909"
     CDS             6068..7723
                     /gene="lldP"
                     /locus_tag="ACHXOL_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR6808897.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7723..8499
                     /gene="lldR"
                     /locus_tag="ACHXOL_001910"
     CDS             7723..8499
                     /gene="lldR"
                     /locus_tag="ACHXOL_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR6808898.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8496..9686
                     /gene="lldD"
                     /locus_tag="ACHXOL_001911"
     CDS             8496..9686
                     /gene="lldD"
                     /locus_tag="ACHXOL_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR6808899.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9870..10343
                     /gene="trmL"
                     /locus_tag="ACHXOL_001912"
     CDS             9870..10343
                     /gene="trmL"
                     /locus_tag="ACHXOL_001912"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR6808900.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10396..11217)
                     /gene="cysE"
                     /locus_tag="ACHXOL_001913"
     CDS             complement(10396..11217)
                     /gene="cysE"
                     /locus_tag="ACHXOL_001913"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR6808901.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11297..12316)
                     /gene="gpsA"
                     /locus_tag="ACHXOL_001914"
     CDS             complement(11297..12316)
                     /gene="gpsA"
                     /locus_tag="ACHXOL_001914"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR6808902.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12316..12783)
                     /gene="secB"
                     /locus_tag="ACHXOL_001915"
     CDS             complement(12316..12783)
                     /gene="secB"
                     /locus_tag="ACHXOL_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR6808903.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12846..13097)
                     /gene="grxC"
                     /locus_tag="ACHXOL_001916"
     CDS             complement(12846..13097)
                     /gene="grxC"
                     /locus_tag="ACHXOL_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR6808904.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13239..13670)
                     /locus_tag="ACHXOL_001917"
     CDS             complement(13239..13670)
                     /locus_tag="ACHXOL_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR6808905.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13915..15459
                     /gene="gpmM"
                     /locus_tag="ACHXOL_001918"
                     /gene_synonym="pgmI"
     CDS             13915..15459
                     /gene="gpmM"
                     /locus_tag="ACHXOL_001918"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR6808906.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15493..16752
                     /gene="envC"
                     /locus_tag="ACHXOL_001919"
     CDS             15493..16752
                     /gene="envC"
                     /locus_tag="ACHXOL_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR6808907.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16756..17715
                     /locus_tag="ACHXOL_001920"
                     /pseudo
     CDS             16756..17715
                     /locus_tag="ACHXOL_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
     gene            complement(17702..>17844)
                     /locus_tag="ACHXOL_001921"
     CDS             complement(17702..>17844)
                     /locus_tag="ACHXOL_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR6808908.1"
                     /translation="MYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFN
                     LR"
BASE COUNT         4474 a         4620 c         4670 g         4080 t
ORIGIN      
        1 aatgcttaaa acaatattta atcgacacac atcactgtca ttaatgtaaa gcaacataga
       61 tcaccaataa tacaatggac aactctaccg gcattgatcg ctggtcggag gaaataagga
      121 tttttacatg aacttgaaga agattttttt cagtgctgtc actgtttcag ttctctgcgc
      181 attaacggga tgtgactaca ttgaagaagg caagccagaa agtagtttat tgaaacaaca
      241 agaagaacat taaaattgat ctacttgaaa aacaacaagc acagctaaag agtcaactgg
      301 aaactattca aaaacaacaa actggcataa taagcagtac aaagacttta actcacgtaa
      361 ttaaatccgt taaagatcaa caaaacactt ttatttttac cgagtttaac ccagcaaaaa
      421 cgaagtattt catcctgaac aatggttcgg tggctttagc gggtcgagtg ttatccattg
      481 acgccacaga gaatggcagt gttattcata tttcactggt caacttatta agtacaccta
      541 tctcaaatat tggttttaat gcgacatggg gtggtgaaaa acctgtcgat gccaaagagt
      601 ttgcccggtg gcaacaatta cttttcaaca catcaatgaa atccacattg aaattattac
      661 caggtcaatg gcaagacatt aatttgaccc tgaagggtgt atcgcccaat aacctgggat
      721 atctgaagtt agccatcaac atggaaaata ttcagttcga caatcttccc tctgctgaaa
      781 atcggcagaa aagaagcaaa aaataaatgc agttagtacg cttataacaa ggaatagcga
      841 gcatcattaa tgaacaaaat atttaaagtt atctggaacc ctgcgacagg gaattatact
      901 gttaccagcg aaacggcaaa aagccgtggc aagaaatctg ggcgcagtaa gctgttaatt
      961 tctgcgctgg ttgcgggtgg aatgttgtcg tcgtttgggg cattggcgaa tgccgggaat
     1021 gacaacggcc agggtgttga ttacggtagt ggatcagctg gcgacagctg ggttgctata
     1081 ggcaaagggg cgaaagcaaa tacttttatg aacaccagtg gttccagtac tgctgtgggt
     1141 tatgacgcta tagctgaagg ccaatatagc tctgccatcg ggtcaaaaac ccatgcgatt
     1201 ggtggtgcat caatggcctt tggggttagt gcaatatcag aaggcgatag aagtatagca
     1261 ctgggtgcct cttcgtattc attgggccaa tactcaatgg ccctcggccg ttattcaaaa
     1321 gcattgggta aattgtctat tgctatgggg gactcttcca aagcggaagg agcaaacgcc
     1381 attgccctgg gaaatgccac taaagctact gagattatga gtattgctct tggcgacacc
     1441 gccaatgcgt caaaagcgta ttcaatggcg ctgggagcaa gtagcgtcgc atctgaagaa
     1501 aacgcaattg ccctggggcg tagcagtgta gctagcggta ctgacagcct cgcatttggc
     1561 agacaatcac ttgccagcgc agcgaacgct attgcgatag gtgctgagac cgaagccgct
     1621 gaaaatgcaa ctgctattgg caataatgcg aaggcaaaag ggactaatag catggcaatg
     1681 gggttcggaa gccttgccga taaagtcaat actatcgcat taggaaatgg cagccaggct
     1741 ctggcagata atgcaatcgc cataggctag ggcaacaaag ctgatggcgt ggatgccatc
     1801 gctctgggta atggtagcca gtcgagaggc ttaaacacca ttgccttagg cacagccagt
     1861 aatgcaactg gtgataagag tcttgcgctt ggtagtaata gcagtgccaa cggtattaac
     1921 tctgtcgcgc tgggcgcaga ttccattgcg gatttagaca ataccgtctc tgtcggcaat
     1981 agttcattaa aacgcaagat cgttaatgtg aaaaatggcg cgatcaagtc tgacagttac
     2041 gatgccatta atggttcaca gctttatgcc attagcgact cggtagcaaa aacgcttgga
     2101 ggaggggctg cagtagatgt tgatgacggt actgttacag caccaaccta caatttaaaa
     2161 aatggtagca aaaataacgt aggggctgcg ctcgctgtac ttgatgaaaa caccctgcaa
     2221 tgggaccaaa ccaaaggcaa atacagcgct gctcatggta ctagtagccc aactgccagc
     2281 gtaatcaccg atgttgcgga tggcacgatt tcagcctcca gtaaggatgc ggttaacggt
     2341 tcccaactga aagctaccaa tgacgatgtc gaagccaaca ccgccaatat cgctactaat
     2401 accagcaaca ttgccacgaa tacagcaagt attgccacca ataccaccaa tatcaccaac
     2461 ctgacggatt ccgttggtga ccttcaggct gatgccctgc tctggaacga aactaaaaag
     2521 gcattcagtg cagctcacgg ccaggatacc accagcaaaa tcaccaacgt taaagatgcc
     2581 gacctgacgg ctgacagcac tgatgctgtt aacggctctc agctgaaaac caccaacgat
     2641 gctgtggcga cgaataccac caatatcgcc aataacactt ccaatattgc cactaacacc
     2701 accaacatct ctaatctgac tgagacggtg actaatcttg gtgaggatgc gctgaaatgg
     2761 gataaggaca atggtgtatt cacggcagct catggcaccg agaccaccag caaaatcacc
     2821 aacgttaaag atggcgacct gacgactggc agcaccgatg ccgttaacgg ctctcagctg
     2881 aaaaccacca acgatgccgt ggcgacgaat accaccaata tcgccactaa caccaccaac
     2941 atctctaatc tgactgagac ggtgactaat cttggtgagg atgcgctgaa atgggataag
     3001 gacaatggtg tcttcactgc agctcatggc aacaataccg ccagcaaaat caccaatatc
     3061 ctggacggca cagtcactgc aaccagttcc gatgccatta acggtagcca gctttatgac
     3121 ttaagcagca atatcgccac ctacttcggc ggcaatgctt ctgtgaatac tgacggtgtg
     3181 tttaccggtc caacctacaa aatcggtgaa acaaattatt ataacgtcgg cgatgcactg
     3241 gctgcgatta actcctcatt tagcacgtct ctcggcgatg ctctgctttg ggatgccacc
     3301 gcaggtaaat tcagtgccaa acacggtact aatggtgacg caagcgtgat cactgatgtc
     3361 gcagatggtg aaatttcaga ctccagttct gacgcagtaa acggctcaca actccacggc
     3421 gtgagcagtt atgttgttga tgcgctgggg ggtggtgccg aagtcaatgc agacggcacc
     3481 atcactgcgc cgacgtacac cattgctaat gctgattacg ataatgtcgg tgatgccctg
     3541 aatgctatcg ataccactct tgacgacgct ctgctctggg atgaggacgc cggtgaaaat
     3601 ggtgcattta gcgccgctca cggaaaagat aaaactgcca gtgtaatcac taacgtcgct
     3661 aacggtgcaa tctctgctgc cagcagcgac gcgattaacg gctcacaact ctataccacc
     3721 aataagtaca tcgctgatgc gctgggtggt gacgcagaag tcaacgctga cggcaccatc
     3781 accgcaccga cttacaccat tgcgaacgcc gagtacaaca acgtcggtga cgccctggat
     3841 gcgcttgatg ataacgccct gctgtgggat gagactgcca atggcggtgc tggagcctac
     3901 aatgccagcc atgacggtaa agccagcatc atcactaatg tcgctaatgg cagtattagt
     3961 gaggacagta ccgatgcagt gaacggttct cagttgaatg cgacgaatat gatgattgag
     4021 cagaacaccc aaattatcaa tcagctcgct ggtaacaccg acgcaaccta tatcgaagaa
     4081 aatggcgctg gtatcaacta cgttcgtact aacgacaacg atttagcctt taacgatgca
     4141 agcgcctctg gtgtcggcgc tacagctgta ggttataacg ctgtcgcgtc tggtgccagc
     4201 agcgtagcca ttggtcagaa cagcagcagc accgttgata ccggtattgc gctgggtagc
     4261 agctccgttt ccagccgtgt gattgccaaa ggttctcgtg acactagcgt aacggaaaat
     4321 ggcgtggtga ttggttatga caccactgac ggcgaactgc taggtgcatt gtcaattggt
     4381 gatgacggta aataccgcca aatcatcaac gtagctgatg gttcagaagc ccatgacgcc
     4441 gttacggttc gccagttgca gaacgctatt ggagcggtcg ccactacgcc aaccaagtac
     4501 tttcacgcca actcaacggc agaagactca ctggccgttg gtgaagactc gctggcaatg
     4561 ggtgcgaaaa ctgtcgttaa tggtaatgca ggtattggta tcggtttgaa cactctggtt
     4621 ctagctgatg cgatcaacgg cattgctatc ggcagtaacg cacgcgcaaa ccatgcaaac
     4681 agtatcgcaa tgggtaacgg ttctcagacc acccgtggtg cacagactgg ctacaccgcc
     4741 tacaacatgg acgcaccgca gaactctgta ggtgagtttt ctgtcggtag tgaagacggt
     4801 caacgtcaga tcacaaacgt cgcagctggt tcagcggata ccgatgcggt taacgtgggt
     4861 cagttgaaag tcactgatga gcgcgtagcg caaaataccc agagcattac taacctgaac
     4921 aatcaggtca ctaatctgga tactcgcgtt actaatatcg aaaacggtat tggcgacatt
     4981 gtcaccaccg gtagcaccaa gtacttcaag accaacaccg atggcgtaga tgccaacgcc
     5041 cagggtaaag atagcgttgc tattggttct ggttccattg ctgccgctga caacagcgtc
     5101 gcactgggta ccggttccgt tgcagaggaa gaaaatacaa tctctgtagg ttcttctact
     5161 aaccaacgcc gtatcaccaa cgtagctgcc ggtgttaatg ccaccgatgc ggttaacgtg
     5221 tcgcagctga aatcttctga agcaggcggc gtgcgctacg acaccaaagc tgatggttct
     5281 atcgactata gcaatatcac cctcggtggt ggcaacggca gtacgactcg tatcagcaac
     5341 gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg cgcagttgaa gcaaagcgtg
     5401 caggaaacga agcaatacac cgatcagcgg atggttgaga tggataacaa actgtctaaa
     5461 actgaaagca agttgagcgg tggtatcgct tctgcaatgg caatgaccgg tctgccgcag
     5521 gcttatacac cgggtgccag catggcttct attggtggcg gtacttacaa cggtgaatcg
     5581 gcagttgctt taggtgtatc gatggtgagc gccaatggtc gttgggtcta caaattacaa
     5641 ggtagtacca atagccaggg tgaatactcc gccgcactcg gtgccggtat tcagtggtaa
     5701 tgatccatta agttagtgtg actaagggca aacgccaaat tgcctgatgc gttacgcgca
     5761 tcaggcaaga taaggcgccc cgggtcaaca atctaagggc aattctctga tgaggattgc
     5821 ccttttcttt accagacatc tccccccaca agaattggcc ctaccaattc ttcgcttatc
     5881 tgacctctgg ttcacaattc cccaattaaa actcacatca atattgccaa tacataacat
     5941 ttagttaacc attcattgtc attatcccta cacaacacaa ttggcagtgc cacttttaca
     6001 caacgtgtga caaggagatg agcaacagac tcattacacg atgtgcgtgg actccaggag
     6061 acctgcaatg aatctctggc aacaaaacta cgatcccgcc gggaatatct ggctttccag
     6121 tctgatagca tcgcttccca tcctgttttt cttctttgcg ctgattaagc tcaaactgaa
     6181 aggatacgtc gccgcctcat ggacggtagc aatcgccctt gccgtggctt tgctgttcta
     6241 taaaatgccg gtcgctaacg cgctggcctc ggtggtttat ggcttcttct atggtttgtg
     6301 gcccatcgcg tggatcatta ttgcagcagt gttcgtctat aagatctcgg tgaaaaccgg
     6361 gcagtttgac atcattcgct cgtctattct ttcgataacc cctgaccagc gtctgcaaat
     6421 gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga gccgcaggct ttggcgcacc
     6481 ggtagcaatt accgccgcat tgctggtcgg cctgggtttt aaaccgctgt acgccgccgg
     6541 actgtgcctg attgttaaca ccgcgccagt ggcatttggc gcgatgggca ttccaatcct
     6601 ggttgccgga caagtaacag gcatcgacag ctttgagatt ggccagatgg tggggcggca
     6661 gctaccgttt atgaccatta tcgtgctgtt ctggatcatg gcgattatgg acggctggcg
     6721 cggtatcaag gagacgtggc ctgcggtcgt ggttgcgggc ggctcgtttg ccatcgctca
     6781 gtaccttagc tctaacttca ttgggccgga gctgccggac attatctctt cgctggtatc
     6841 actgctctgc ctgacgctgt tcctcaaacg ctggcagcca gtgcgcgtat tccgttttgg
     6901 tgatttgggg gcgtcaaaga ttgatatgac gctggcccac accggttaca ctgcgggtca
     6961 ggtgttacgt gcctggacac cgttcctgtt cctgacagct accgtaacac tgtggagcat
     7021 cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg tatgagtggg tgatcaacat
     7081 tccggtgccg tacctcgata aactggttgc ccgtatgcca ccagtggtca gcgaggctac
     7141 agcctatgcc gccgtgttta agtttgactg gttctctgcc accggcaccg ccattctgtt
     7201 tgctgcactg ctctcgattg tctggctgaa gatgaaaccg tctgacgcta tcagcacctt
     7261 cggcagcacg ctgaaagaac tggctctacc catctactcc atcggcatgg tgctggcgtt
     7321 cgcctttatc tcgaactatt ccggactgtc atcaacactg gcactggcac tggcgcacac
     7381 cggtcatgca ttcaccttct tctcgccgtt cctcggctgg ctgggggtat tcctgaccgg
     7441 gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa gccaccgcag cacaacaaat
     7501 tggcgtctct gatctgttgc tggttgccgc caataccacc ggtggcgtca ccggtaagat
     7561 gatctccccg caatctatcg ctatcgcctg tgcggcggta ggcctggtgg gcaaagagtc
     7621 tgatttgttc cgctttactg tcaaacacag cctgatcttc acctgtatgg tgggcgtgat
     7681 caccacgctt caggcttatg tcttaacgtg gatgattcct taatgattgt tttacccaga
     7741 cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga ttgatgaaaa aaacctggaa
     7801 gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc aactcggcgt atcacgtaat
     7861 tcactgcgcg aggcgctggc aaaactggtg agcgaaggcg tgctgctcag tcgacgcggc
     7921 ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg agcaaaacat cgtccagccg
     7981 ctaaaaacgc tgatggccga tgatccggat tacagtttcg atattctgga agcccgctac
     8041 gccattgaag ccagcaccgc atggcatgcg gcaatgcgcg ccacacctgg cgacaaagaa
     8101 aagattcagc tttgctttga agcaacgcta agtgaagacc cggatatcgc ctcacaagcg
     8161 gacgttcgtt ttcatctggc gattgccgaa gcctcacata acatcgtgct gctgcaaacc
     8221 atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc atagccgtca gcggatgtat
     8281 ctggtgccac cggttttttc acaactgacc gaacaacatc aggctgtcat tgacgccatt
     8341 tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg cgcaccttag ttttgttcac
     8401 accaccatga aacgattcga tgaagatcag gctcgccacg cacggattac ccgcctgccc
     8461 ggtgagcata atgagcattc gagggagaaa aacgcatgat tatttccgca gccagcgatt
     8521 atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt ccactatatg gatggtggtg
     8581 catattctga atacacgctg cgccgcaacg tggaagattt gtcagaagtg gcactgcgcc
     8641 agcgtattct gaaaaacatg tccgacttaa gcctggaaac gacgctgttt aatgagaaat
     8701 tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg catgtatgcg cgtcgtggcg
     8761 aagttcaggc agccaaagcg gcggacgcgc atggcattcc gtttactctc tcgacggttt
     8821 ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg cccaatgtgg ttccagcttt
     8881 atgtactgcg cgatcgcggc tttatgcgta acgcgctgga gcgagcaaaa gcagcgggtt
     8941 gttcgacgct ggttttcacc gtggatatgc cgacaccggg cgcacgctac cgtgatgcgc
     9001 attcaggtat gagcggccca aatgcggcaa tgcgccgcta cttgcaggcg gtgacgcatc
     9061 cgcaatgggc gtgggatgtg ggcctgaacg gtcgtccaca tgatttaggt aatatctcag
     9121 cttatctcgg caaaccgacc ggactggaag attacatcgg ctggctgggg aataacttcg
     9181 atccgtccat ctcatggaaa gaccttgagt ggatccgcga tttctgggat ggcccgatgg
     9241 tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc agtacgtttt ggtgctgatg
     9301 gaattgtggt ttctaaccac ggtggccgcc agctggacgg tgtactctct tccgctcgtg
     9361 cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc cattctggcg gatagcggaa
     9421 ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg tgccgacacc gtactgctgg
     9481 gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc gggtgtagct aacctgctaa
     9541 atctgatcga aaaagagatg aaagtggcga tgacgctgac tggcgcgaaa tcgatcagtg
     9601 aaattacgca agattcgctg gtgcaggggc tgggtaaaga gttgcctacg gcactggctc
     9661 cgatggcgaa agggaatgcg gcatagtcgt ttgcgccccc tcaccctcag ggagagggga
     9721 ccgttcggcg ctgtatgtac tccctcactc tgaaacgaca ccgcactctt tttttctccc
     9781 tcgcccctcc ggggagggcc ggggtgaggg gaaaaggccg cacggttcac caattttctg
     9841 ctatcctgcc cccgcactaa gggggcagta tgctaaacat cgtactttac gaaccagaaa
     9901 ttccgccaaa tactggcaac atcatccgtc tttgcgctaa taccggcttt cgtctgcata
     9961 tcatcgaacc gatgggattt gcctgggacg ataagcgcct gcgccgcgcg gggctggact
    10021 atcacgagtt taccgccgtt acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa
    10081 atccccagcg cctgtttgcc ctcaccacga aaggtacgcc tgctcacagc gccgtaagct
    10141 atcaggatgg cgattatctg atgttcggcc cggaaacacg cggcctgcca gcgagcattc
    10201 ttgatgccct gcccgctgaa caaaaaattc gcattccgat ggtgccggac agccgcagca
    10261 tgaatctgtc caatgcggtg tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc
    10321 cgggagcgtt gttgagagat tagttactgt atgccggata gatgattaca tcgcatccgg
    10381 cacgatcaca ggacattaga tcccatcccc atactcaaat gtatggttaa taccgttgaa
    10441 atgctggtcc atatccattg atggcttatc gctgtctggt ttaccgacaa tacgagccgg
    10501 aacgccagcg gcggttgtat gcggcggcac cggttgcagc accacggaac ctgcgccaat
    10561 cttcgcgccg cgaccaactt caatattgcc gaggattttc gcgcccgcgc caatcatcac
    10621 accttcacga attttcgggt gacggtcacc accagattta cccgtaccgc caagcgtcac
    10681 agattgcaga atcgatacgt cgttttcaat taccgccgtt tcacccacaa cgatgcccgt
    10741 ggcgtggtcg agcataatgc cacggccaat ttttgctgcc gggtgaatat cgacctggaa
    10801 cgtcacagaa acctggtttt gcagaaagat tgccaatgcg cgacgcccct gattccacaa
    10861 ccagtgaccg atgcgatagg cctgcaaggc atgaaaaccc ttcaggtata acaacggggt
    10921 tgagtattta tcgactgccg ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga
    10981 ggcgatcatt tccgggtcag cggcgtaggc ttcttccacc acttcacgga tagcaatagc
    11041 aggcataatt ggcgatgaca gcttgttcgc cagcatgtag ctcagtgcac taccaaggtt
    11101 ttcgtgcttg agtagcgtcg cgtggtaaaa actggccagc attggctcac agtccgccag
    11161 cgttctggct tcggctttaa tattgttcca gacaatttcc agttcttcac acgacattgc
    11221 ttactccaca cgatgagata atgaccggcc cgttctgcgc gggccgggtc atagcggtaa
    11281 caaaggttcc ctggggttag tggctgctgc gctcgtcctt gcgtgcacga cctaataacg
    11341 tcaatgctgc ctcgcgcgcg ttttttccgc aatataatac ttgataaatt tcctcggtta
    11401 ttggcatttc tacgccgaag cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc
    11461 cttccaccac ctgaccaatc ttctcctgcg cgctttgtac atccatgccc tgaccgagca
    11521 tcatgccaaa acggcggtta cgcgactggt tgtcggtaca ggtaagcacc agatcgccca
    11581 gccccgccat gcccataaag gtggcaggat cggcacccag cgccgcgcca agacgcgaca
    11641 tttcagccag cccacgggtg atcagtgccg tacgcgcatt cgcaccaaaa ccgataccgt
    11701 cggacatccc cgcgccaatg gcaatgacgt ttttcaccgc accgccaagc tgcacaccga
    11761 tgaaatccgg gttgctgtaa acgcggaaac ttttgccaca gtgcagcaat tgttggagat
    11821 catcggcaaa ggtctggtcg gtagaggcca acgaaatagc cgtcggtaaa cctgccgcca
    11881 gttctttcgc aaacgttggg ccagagataa ccgccagcgg aatttgatcg cctaaggcct
    11941 cacgcgccac gtcctgtaac agacgtccgg tttccgcttc cagccctttg gtcgcccaca
    12001 ccagacgcgc atcaggacgc atcagcggtt taatctggcg cagcacttca ccaaagacat
    12061 ggctgggtac gacgacgaga atattacggc tggctgccag cgcagtggcg agatcgcttt
    12121 caagatggag cgtatcggga aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc
    12181 gttcaagcgt tgcgatatgt tcagggtcat ggccccagag gacaacctcg tggccatttc
    12241 ttgccagggt aatggcaaga gcggtgccgt acgagccggc accgatcaca gtcattgaag
    12301 cattacgttg gttcatcagg catcctgatg ttcttcagta ccttcgccag cctgctgctg
    12361 caaatagttc atgaacagcg catcgaagtt aatcggcgca aggttcagtt gcgggaatgt
    12421 accgcgggat accacgctgg tgatgcactc acgagcatac gggaacagaa tgttcgggca
    12481 gtatgcgccc aggcaatgtg ccatctgggt gccttcgata cccgcgatgg agaaaatacc
    12541 gccctgctga acttcacaca ggaacgcggt ttcttcgccc agggaggccg ttacggtaac
    12601 acgcagtacc acttcgtata cgtcatctgc cagttgggaa gatgccgtat ccagatcaag
    12661 tttaacttct ggttgccaat ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga
    12721 gatatccttg gtataaatac gttggatctg gaaagtcatt tcagtgttgt tttgttctga
    12781 catgtgtaga aaacccttaa gtgttgtcct taaatactgc gcaatgtaat gccaacgttc
    12841 acacgttatt tcagcagggg atccagtcca ccacgtgcat ccaatgcata caagtcatca
    12901 cagccgccaa tgtgctgtgc gtcaataaaa atctggggaa ccgtggtgcg accgctgcgt
    12961 ttgatcatct cttcacgctt ggcggcattg ccatcgatcg gcaactcctg aaaactcacg
    13021 cccttgctgc tcagcagtgc ttttgcacga tggcaatacg ggcaggtttc tttggtatag
    13081 atttcaacat tggccataat ttagctcctg tttttttacc ctgaaatttc atgttgcagg
    13141 gcggctgcaa aatggagtgt ccccagaagc ttataacaat aagtcgctgg gggcctaatt
    13201 gcagctaacg gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccagaggc
    13261 aagttttcgc cagcccagcc agcaacgccc tctttcagca cgaatacttg cgcaaaacca
    13321 gctttcgtca gcgcgtttgc aggctcctgg cactgcatgc cagaaccgtc taccacgata
    13381 accggtttgt ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc
    13441 aacagattga tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc
    13501 acaaccacag cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc
    13561 actttcgagg tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag
    13621 gcgatactca gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac
    13681 aactcccgac gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa
    13741 tacagccagc gcgttaactg gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa
    13801 tctggcatcg gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga
    13861 ttcagctgta gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg
    13921 gtttctaaaa aacctatggt actggtgatt ctggatggct atggctatcg tgaagaacag
    13981 caggataacg ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt
    14041 ccgcataccc tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt
    14101 aactccgaag taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact
    14161 cgtctggacg ttgaaatcaa agatcgcgct ttctttgcta atccagtgct gactggcgcg
    14221 gtagataaag cgaaaaacgc aggtaaagca gtacacatta tgggtctgct ctccgcaggc
    14281 ggcgtacaca gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc
    14341 gcagaaaaaa tctacctgca cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct
    14401 gaatcctcgc tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg
    14461 tccatcattg gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa
    14521 gcctatgacc tgctgactct ggcgcagggt gagtttcagg ccgatactgc cgttgctggt
    14581 ttgcaggcag cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt
    14641 gcagaaggtc agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc
    14701 cgtgctgacc gcgcgcgtga aatcactcgt gcattcgtga acgctgattt cgatggcttc
    14761 gcgcgtaaga aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc
    14821 aaaactgcgg ttgcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg
    14881 aaaaacgaca aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca cgttactttc
    14941 ttcttcaacg gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca
    15001 ccgaaagtgg ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa
    15061 ctggttgcgg ccatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc
    15121 gacatggtag gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcactggat
    15181 cactgcgtgg aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc
    15241 gctgaccacg gtaacgctga acagatgcgc gatccggcaa cgggtcaggc acacacggca
    15301 cacaccaacc tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgca
    15361 ggcggcaaac tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg
    15421 caagagatga ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag
    15481 gcgattaata ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc
    15541 tacgccagcg ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag
    15601 cgtgaccaac tcaaatctat tcaggccgat atcgccacaa aagagcgcgc ggtacgccaa
    15661 aagcaacaac aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct
    15721 gaagccaccc gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa taaacagatt
    15781 gatgagatga acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc
    15841 ctcgccgcac aactggatgc cgcattccgt cagggcgagc ataccggtat tcagctaatt
    15901 ctcagcggtg aagaaagcca gcgtggacag cgtttacagg cttatttcgg ctatctcaac
    15961 caggcgcgac aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt cgccatgcag
    16021 cgtgctgaac tggaagagaa acaaagcgag caacaaacgc tgttatatga acagcgcgcc
    16081 caacaggcga aactgactca ggctctgaac gagcgtaaaa agacgctggc ggggctggaa
    16141 tcttccatcc agcaaggtca gcagcagcta agtgaactgc gtgccaacga atcccgtctg
    16201 cgtaacagta ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt
    16261 gaggctcagg cagttcgcga caggcagaaa gaagcgacgc gcaaaggcac cacctacaaa
    16321 ccgaccgaaa gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt
    16381 caggcattct ggccagttcg tggccctacg ctgcatcgct atggcgagca gctacagggt
    16441 gaattacgct ggaaaggaat ggttatcggt gcctctgaag gtactgaagt taaagcaatt
    16501 gccgacggtc gggtgattct ggctgactgg ctgcaaggct acggtctggt ggtggtggtt
    16561 gagcacggta aaggcgatat gagtctttac ggctataatc aaagcgcgct ggtgagcgtt
    16621 ggttcgcagg ttcgtgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggc
    16681 agaccttcac tctatttcga aattcgccgc cagggtcagg ccgtcaatcc acagccgtgg
    16741 ttgggaagat aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg
    16801 gcgctctcct cccctgtaat tgctggcaaa cttgccatcg tcattgatga ttttgggtat
    16861 cgcccgaaca acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta
    16921 cccgattcac cgcacgcctg agagatggcg accaaagcgc ataacagcgg tcacgaagtg
    16981 ttgattcatc tcccgatggc accgttgagt aaacagccgc tggagaaaaa tacgctgcgc
    17041 ccggagatga gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc
    17101 tatgccgtgg ggatcaataa ccacatgggc agcaagatga cctctaacct gtttggtatg
    17161 cagaaagtga tgcaggcgct ggagcgttat aatctttact tcctcgacag cgtaaccatt
    17221 ggtaataccc aggcgatgcg cgccgcgcaa ggcactggcg tgaaggtgat caaacggaag
    17281 gtgttcctcg acgattcaca gaacgaagcg gacatccgtg tgcaattcaa tcgcgcaatt
    17341 gacctagcgc gtcgcaacgg atcaaccatt gccattgggc atcctcaccc ttcaacggta
    17401 cgcgtgttgc aacagatggt ttataacctg ccgccagaca ttacgctggt gaaagccagc
    17461 agcttgctga atgaaccgca ggttgatacg tctacaccgc cgaaaaacac tgtgcccgac
    17521 acaccgcgta atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct
    17581 gtttatgcta atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc
    17641 gtttacttcc agcatcagtg gcaaggctgg ggcaaacagc ctgaagcggc gaagcttaac
    17701 gctagcgtaa attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc
    17761 attgccataa gcggaaagac cacagtaacg cctgataacc cacctttacg ctgcgcacat
    17821 tggtaatcag gcgcttatac atac
//
LOCUS       DAZRUF010000072        17765 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZRUF010000072 DAZRUF010000000
VERSION     DAZRUF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17765
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.072"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(57..1031)
                     /gene="cysB"
                     /locus_tag="ACHXOL_001922"
     CDS             complement(57..1031)
                     /gene="cysB"
                     /locus_tag="ACHXOL_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR6808909.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1241..3838)
                     /gene="topA"
                     /locus_tag="ACHXOL_001923"
     CDS             complement(1241..3838)
                     /gene="topA"
                     /locus_tag="ACHXOL_001923"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR6808910.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4218..4469
                     /locus_tag="ACHXOL_001924"
     CDS             4218..4469
                     /locus_tag="ACHXOL_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR6808911.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4505..5554)
                     /gene="sohB"
                     /locus_tag="ACHXOL_001925"
     CDS             complement(4505..5554)
                     /gene="sohB"
                     /locus_tag="ACHXOL_001925"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR6808912.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5774..6532
                     /locus_tag="ACHXOL_001926"
     CDS             5774..6532
                     /locus_tag="ACHXOL_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR6808913.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6529..7119
                     /gene="cobO"
                     /locus_tag="ACHXOL_001927"
     CDS             6529..7119
                     /gene="cobO"
                     /locus_tag="ACHXOL_001927"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR6808914.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7159..8031)
                     /gene="rluB"
                     /locus_tag="ACHXOL_001928"
     CDS             complement(7159..8031)
                     /gene="rluB"
                     /locus_tag="ACHXOL_001928"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR6808915.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8244..9358)
                     /locus_tag="ACHXOL_001929"
                     /pseudo
     CDS             complement(8244..9358)
                     /locus_tag="ACHXOL_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9386..10006)
                     /locus_tag="ACHXOL_001930"
     CDS             complement(9386..10006)
                     /locus_tag="ACHXOL_001930"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR6808916.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10003..10884)
                     /gene="rnm"
                     /locus_tag="ACHXOL_001931"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(10003..10884)
                     /gene="rnm"
                     /locus_tag="ACHXOL_001931"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR6808917.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11022..11066
                     /gene="trpL"
                     /locus_tag="ACHXOL_001932"
     CDS             11022..11066
                     /gene="trpL"
                     /locus_tag="ACHXOL_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR6808918.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11043..11136
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11158..12720
                     /gene="trpE"
                     /locus_tag="ACHXOL_001933"
     CDS             11158..12720
                     /gene="trpE"
                     /locus_tag="ACHXOL_001933"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR6808919.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12720..14315
                     /gene="trpD"
                     /locus_tag="ACHXOL_001934"
     CDS             12720..14315
                     /gene="trpD"
                     /locus_tag="ACHXOL_001934"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR6808920.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14316..15677
                     /gene="trpCF"
                     /locus_tag="ACHXOL_001935"
     CDS             14316..15677
                     /gene="trpCF"
                     /locus_tag="ACHXOL_001935"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR6808921.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15689..16882
                     /gene="trpB"
                     /locus_tag="ACHXOL_001936"
     CDS             15689..16882
                     /gene="trpB"
                     /locus_tag="ACHXOL_001936"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR6808922.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16882..17688
                     /gene="trpA"
                     /locus_tag="ACHXOL_001937"
     CDS             16882..17688
                     /gene="trpA"
                     /locus_tag="ACHXOL_001937"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR6808923.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4251 a         4720 c         4453 g         4341 t
ORIGIN      
        1 gagaatatat tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat
       61 ttttccggca gttttatatc tttaaacatg acctcaattt cttcattaga gcgcaatgcg
      121 acagccgcat caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac
      181 atataactgc gcaagaaagt actacggcga aaaccaattt tggttgtact gtggctgaag
      241 atatcgtgag catcaacacg cacaaggtcg ggatcggcga ccggatccac cgccatgctg
      301 gcaatgaccc ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt
      361 gccgtgaaga cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa
      421 cgtccggtaa agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta
      481 atggcttttt tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg
      541 tagcacggta acatcactaa atcttcatac agatgcagcg cttctgtggc gatagcgaaa
      601 tcagcattgc ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc
      661 aaagaaacgc ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt
      721 gcctgggtat gcgtggtggc gatatacagt gagcctttat ccggccaggt gtgctctccg
      781 gcaaccgatt ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct
      841 tgccctgctg gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct
      901 agctcgtctt ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct
      961 tccgctgttg atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt
     1021 tgtaatttca tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac
     1081 ctggcgggtg actaatacga tttaataata aaaaaggtgc taactataac cactatatca
     1141 tttatagcct tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt
     1201 tcgcgaccct ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc
     1261 atttgccatc aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga
     1321 catattgctg tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct
     1381 gctgtggcgc atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa
     1441 gctcttccac cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga
     1501 aaacacccgc agcgccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct
     1561 caggtaatgg caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac
     1621 gtgtgttttt acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca
     1681 ggtgcatttc agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc
     1741 ggaattcgcc ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca
     1801 acttacgttt cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac
     1861 gttttgcgcg cagcgcgttg gtttcagcgt cttcgccttc cagtacgttc agcacttcgt
     1921 tttccggcac caggttaatg gtggttttgc aacgctcttt cggcggcagc gcataaccag
     1981 aacagccaag gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaagtcg
     2041 ggcagtcaat gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt
     2101 tttcagcttt atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc
     2161 actctgcttc gtgatttgcc accaggtcga ggctgttttc catctgtgcg gtgaagtcgt
     2221 agttcattaa ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg ccaatttttt
     2281 ccgcatagaa acgacgattt tctactcgca cgtagccacg atcctgaatg gtcgaaatga
     2341 tcgacgcata ggtagacgga cgaccgatac cgcgtttttc cagctcttta accagcgatg
     2401 cttcactgaa acgggctggc ggcttggtaa agtgctgggc tggtgtaagt tcaacgagcg
     2461 tcagagcatc gcctttatta actgctggta agatgcgatc ttcatcgcct ttacgcaacg
     2521 caggcatcac ttttgtccag ccatcaaaac gcaaaatacg accgcgtgct ttcaggcgga
     2581 aatcgcccgc accaatggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg
     2641 ctacgaactg acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca
     2701 gcgattccgc catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt
     2761 tttctttgct ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc
     2821 tgatataacc acgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca
     2881 tgtaagtgat atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc
     2941 caaatccaag acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag
     3001 gtttactggt tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca
     3061 gacttaccgc agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat
     3121 gagtcacctg taacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc
     3181 agaactcttc cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg
     3241 ccaccgactg cacacgaccg gcagacagac cacgagcgat ctttttccat agcagcggcg
     3301 aaaccatata ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat
     3361 caatattcag ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt
     3421 taaacaccac tcggctatag cgcgcatcat cacccccaat cacttcccgc aggtgccatg
     3481 caatggcttc cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag
     3541 ccagttgttt cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct
     3601 cccaattgtg ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt
     3661 taggcttttt agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac
     3721 tggtcggcaa atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat
     3781 acttgttgat cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat
     3841 tcaccttacc taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg
     3901 caacgagtta ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt
     3961 tcacgaaaat tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg
     4021 gaattttggt cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac
     4081 ctgataaaat ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg
     4141 ctcttgccac cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct
     4201 tgtttaagga gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga
     4261 aagaggccaa caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg
     4321 gcgtcacgca gcgtaatggc gtgctggtat ttatcggcga ctacttttta gatgaacaag
     4381 gattgccaac agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc
     4441 tttcagaaaa atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg
     4501 tcttttacat caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc
     4561 tctctgccgc gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat
     4621 taaccacttc acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat
     4681 caaccaggcc tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt
     4741 caatatccag agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt
     4801 tcagctcttc gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg
     4861 tgcgcttata ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc
     4921 ggttaaagtt gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg
     4981 aaacaatttt gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta
     5041 ccgtaacagt taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc
     5101 cgtaaccatg caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt
     5161 tgaatgctgc gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca
     5221 tgctgccttt aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca
     5281 gtttggcttt cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt
     5341 gccactgttt ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct
     5401 tatactgttc gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag
     5461 caacattgac aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg
     5521 ccaaaaacaa accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc
     5581 tttggctcac agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga
     5641 aagtatgaca aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc
     5701 tgtttcaggc ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg
     5761 ttgaggagta gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct
     5821 ggtgacggga gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg
     5881 tgcgacagtg attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat
     5941 aaacgaagaa actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc
     6001 cgaaaattgc caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt
     6061 tttgcataat gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt
     6121 ctggcaggac gtcatgcagg tcaacgttaa tgccaccttt atgctcaccc aggcactgct
     6181 tcctttatta ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttggacg
     6241 tcagggacga gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat
     6301 gcaggtactg gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg
     6361 tacgcgcacc gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac
     6421 acccgctgat atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac
     6481 cggcatgacc tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa
     6541 cgctaccaac agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc
     6601 caggatgaac gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg
     6661 gcatttggta cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt
     6721 attaaaggca cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt
     6781 caggtgatgg caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc
     6841 tgccgcgaag tctggcaaca tgcaaagcgg atgcttgctg attcctcact ggatatggtt
     6901 ttgcttgatg aactgacgta tatggtggcg tatgactatt tgccactgga agaagtggtg
     6961 caggcgttaa atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat
     7021 cgggatattc ttgaactggc ggatacggta agtgagctgc gccccatcaa acatgcgttt
     7081 gatgccggtg ttaaggcgca aatagggatt gattattaat ctgtaaaaat gccggatatg
     7141 acgtccggca tactttgatt aaccgttatt acgcccgcca gaacgacgac cgccgctcac
     7201 ctgactgtga cgtttcaccg cacgacgaat ctgattagcc ttcatgcgac gacggtcttt
     7261 ttctacagcg actttagagc tggtttctgg cggaagctcc accagttcgc gcagatagtt
     7321 agtctgggcg aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat
     7381 atcaccgtaa cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg
     7441 acgaacttca cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc
     7501 gccgccgctg aacttgatgg ttttaaaagc tgccggacca tcttccagtt gcacgccacg
     7561 actcaaatca cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg cgtattcacg
     7621 ttcaacttca cggcttggat gcattaaacg gtttgccagt tcaccatcgg tggtgaacag
     7681 cagcagacca caggtgttaa cgtccagacg acctacggca atccagcgtg cgccacgcag
     7741 ctttggcaga cggtcaaaca ccgtcggacg cccttccgga tcgttacggg tacacaactc
     7801 accttccggt ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga
     7861 aatcagatga ccatcgatac gaattttcag cccaggggta acttcaacgc gatcgccgag
     7921 tttagcaatt ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg
     7981 gcgggagccg tggccagcgc gggccagcac tttctgtaat ttttcgctca tagagcttcc
     8041 ttttggtgtc gccttcccag gcgtctgata tgcttttgtg caacagtgag tcagaactta
     8101 catctgactc actgatagta aagtctgttt gaactcttca ggccttaagt aaacgactcg
     8161 acgttttaac tggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct
     8221 tgtttcgatt aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgcca
     8281 ccagcaccac caccggaata acttcgcccc aacaaactgg ttttggcccc cagactcagg
     8341 tgcatctgta atgaaaacaa aacgccacta cgggcaattt ccggggcgct aacagccgct
     8401 gtgagtgatg gggtgatacg tgctacccat tctcttccaa ggtttgctga gactgcccaa
     8461 cctagcaacg cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt
     8521 gctgcctctt tacgcgcaag atagttaacg atagcttcac cgtgggcata acgttgctgc
     8581 ccagtttgtg taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg
     8641 aaaacagcgc taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg
     8701 atgtagagag aatagaaggc cacgcctgaa caaatcagtg gtaaacaatt cttccgtcct
     8761 caaggatgcg gccatttcga tatgtgtcgc cctcttctcc agtaaagaga tcgatttcgc
     8821 taaatggatt acccgcgata tcagggaact tgagtgaaaa tcgggtctta tagatttcaa
     8881 acggccagtg gttaccagtc acgttccaga ttaacagatc tgaatctccc tcacgcagga
     8941 aagcattttt aacctgataa tgaatttgat aatgatagtc gccttgaggc aagaaacgtt
     9001 gtttatcgcc cacgacaata ctcattaacc cgatatgcca gtcatgatgc cagggttcag
     9061 gaataccatc acgggtgacg ccaacaatat gatagttcag cagtgcagcg tccccatcag
     9121 gctgcatcca gagtcgtggt aaatcacgat aaaatccgtg tttaatctgc tgatttcgcg
     9181 cctgaaaatg aatattttct ttgatgtcga gcaccccatc cggttgcaga gtggcttcaa
     9241 catcaaagag cgaaatccct tcttcataag aagggatctc cgtgttttct gcataactta
     9301 gagatgatac aaatagccct gctatcaaca gcaaaatgca gcgaaacttc cctgccatag
     9361 tcttccatcc gtggaacgca atttgttata agaaaggctt cacatcacct acgccttcac
     9421 gcaccacgac cggcgtatca tcggtaagat caataaccgt tgtcggtttc tgcccgagat
     9481 agccgccgtg aataatcaaa tctacctgtt tttccagacg gtctttaatt tcttcgggat
     9541 cggattcggt aaattcgctg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca
     9601 gtgcttcaag caacgcctgc gcaatcgggt tagaaggcac acgcataccg atggttttgc
     9661 gtttttcctg caacagacgg cgtggcactt ccttcgtccc cttcaggatg aaggtatagt
     9721 tgcccggcgt gttgtttttc attaaacgaa acgcaacgtt atcaacaaat gaataggtcg
     9781 agagttcaga aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa
     9841 tacgacaaat acgctccatc gcgtttttat cttcaatttt acagccgagc gcatagccgg
     9901 aatcagttgg ataaacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca
     9961 gacgttgctg tgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt
    10021 ggtgttctgc ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag
    10081 tttacgacct agttcgatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca
    10141 taaatgatgc tgacgcgcaa gggccgccag ctgggtacgt tcattgggcg attgctggca
    10201 ctgcgcgact tccatcgcgt caccgtggtg ttcggcaaaa tgcgcaacca atcttttcag
    10261 ccatttagca ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg
    10321 atgaatgaca tcaatagctt gttctattgt acaccactgt ggcggaacgt atccggtttt
    10381 cccgcgcgcc agatattttt taaagacatc cgccattgaa ctggctttgc cgcaccccac
    10441 aaggaaacgc gcaaaatgac cgcgcgtcac cgcaccgccc tgcgccagtc gtcgcgcgcc
    10501 ttccagtgcc cctggtattt gcgctttttc aagtctttcg gcaatcagtt gcgcccgctg
    10561 attgcgccgt tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat
    10621 attcagcccg acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacgccggg
    10681 aataagattc agcgccagtc ccgaacgtga aatttcctct cttgctggca cgattgcagc
    10741 tgtgttgtca tggtcggtga tcgccagggt accgacgcgc atctcgactg cacggtgcac
    10801 caatacttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat
    10861 cactgcgtaa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg
    10921 acatcataac agttctggca aatattctga aatcagctgt tgacaattaa tcatcgaact
    10981 agttaactag tacgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac
    11041 tgaaagcttg gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc
    11101 agatacccag cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg
    11161 caaacacaaa aaccgactct cgaactgcta acctgcgaag gcgcttatcg cgacaacccg
    11221 actgcgcttt ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca
    11281 gatatcgaca gcaaagatga tttaaaaagc ctgctactgg tagacagtgc gctgcgcatt
    11341 acagcattag gtgacactgt cacaattcag gcactttccg gcaacggcga agccctgctg
    11401 gcactactgg ataacgccct gcctgcgggt gtggaaaatg aacaatcacc aaactgccgt
    11461 gtgctgcgct tcccccctgt cagtccactg ctggatgaag acgcccgctt atgctccctt
    11521 tcggtttttg acgctttccg tttattgcag aatctgttga atgtaccgaa ggaagaacga
    11581 gaagcaatgt tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaagattta
    11641 ccgcaactgt cagcggaaaa taactgccct gatttctgtt tttatctcgc tgaaacgctg
    11701 atggtgattg accatcagaa aaaaaacacc cgcattcagg ccagcctgtt tgctccgaat
    11761 gaagaagaaa aacaacgtct cactgctcgc ctgaacgatc ttcgccagca gctgaccgaa
    11821 gccgcgccgc cgctgccggt ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc
    11881 gatgaagagt tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt
    11941 ttccaggtgg tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat
    12001 tacgtgctga aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc
    12061 accctgtttg gcgcgtcgcc ggaaagttcg ctcaagtatg acgccaccag ccgccagatt
    12121 gagatctacc cgattgccgg aacacgtcca cgcggtcgtc gtgccgatgg ctcgctggac
    12181 agagacctcg acagccgcat cgaactggaa atgcgtaccg atcataaaga gctttctgaa
    12241 catctgatgc tggtggatct cgcccgtaat gatctggcac gcatttgcac ccccggcagc
    12301 agctacgtcg ccgatcttac caaagttgac cgttactctt acgtgatgca cctggtctcc
    12361 cgcgtggtcg gtgagctgcg ccacgatctc gacgccctgc acgcttaccg cgcctgtatg
    12421 aatatgggga cgttaagcgg tgcgccgaaa gtacgcgcca tgcagttaat tgccgaggcg
    12481 gaaggtcgtc gccgcggcag ctacggcggc gcggtaggtt atttcaccgc gcatggcgat
    12541 ctcgacacct gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa
    12601 gccggtgctg gcgtagtcct tgattctatt ccgcagtcgg aagccgacga aacccgtaat
    12661 aaagcccgcg ctgtactgcg cgctattgcc accgcgcatc atgcacagga gactttctga
    12721 tggctgacat tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt
    12781 tgcgcagcaa tgggcataat gtggtgattt accgcaacca tattccggcg cagaccttaa
    12841 ttgaacgcct ggcgacgatg agcaatccga tactgatgct ttctcctggc cccggtgtgc
    12901 cgagcgaagc cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgcccatta
    12961 ttggcatttg cctcggacat caggcaattg tcgaagctta cgggggctat gtcggtcagg
    13021 cgggcgaaat tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg
    13081 ccggattaac aaacccgctg ccggtggcgc gttatcactc gctggttggc agtaacattc
    13141 cggccggttt aaccatcaac gcccatttta atggcatggt gatggcagta cgtcacgatg
    13201 cggatcgcgt ttgtggattc cagttccatc cggaatccat tctcaccacc cagggcgctc
    13261 gcctgctgga acaaacgctg gcctgggcgc agcagaaact agagccagcc aacacgctgc
    13321 aaccgattct ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa agccaccagc
    13381 tgttttcagc ggtggtgcgt ggcgagctga agccggaaca actggcggcg gcgctggtga
    13441 gcatgaaaat tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc gcgctactgg
    13501 aaaacgcagc gccgttcccg cgcccagatt atctgtttgc tgatatcgtc ggtactggcg
    13561 gtgacggcag caacagtatc aatatttcta ccgccagtgc gtttgtcgcc gcggcctgtg
    13621 ggctgaaagt ggcgaaacac ggcaaccgta gcgtctccag taaatctggt tcgtccgatc
    13681 tgctggcggc gttcggtatt aatcttgata tgaacgccga taaatcgcgc caggcgctgg
    13741 atgagttagg tgtttgtttc ctctttgcgc cgaagtatca caccggattc cgccacgcga
    13801 tgccggttcg ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg ccattgatta
    13861 acccggcgca tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga
    13921 ttgccgaaac cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga
    13981 tggatgaagt ttcattacac gcgccgacaa tcgttgccga gctgcatgac ggcgaaatta
    14041 agagctatca attgaccgct gaagattttg gcctgactcc ctaccaccag gagcaactgg
    14101 caggcggaac accggaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg
    14161 acgccgccca tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc
    14221 atgaagatct gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt
    14281 acgacagagt taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa
    14341 tcgtcgcaga caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt
    14401 ttcagaatga ggttcagccg agcacgcgac atttttatga tgcgctacag ggtgcacgca
    14461 cggcgtttat tctggagtgc aagaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt
    14521 tcgatccggc acgcattgcc tccatttata aacattacgc ttcggcaatt tcggtgctga
    14581 ctgatgagaa atattttcag gggagctttg atttcctccc catcgtcagc caaatcgccc
    14641 cgcagccgat tttatgtaaa gacttcatta tcgaccctta ccagatctat ctggcgcgct
    14701 attaccaggc cgatgcctgc ttattaatgc tttcagtact ggatgacgac caatatcgcc
    14761 agcttgcagc cgtcgctcac agtctggaga tgggggtgct gaccgaagtc agtaatgaag
    14821 aggaactgga gcgcgccatt gcattggggg caaaggtcgt tggcatcaac aaccgcgatc
    14881 tgcgtgattt gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca
    14941 acgtgacggt aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc
    15001 acttcgctaa cggttttctg attggttcgg cgttgatggc ccatgacgat ttgcacgccg
    15061 ccgtgcgccg ggtgttgctg ggtgagaata aagtatgtgg tctgacgcgt gggcaagatg
    15121 ctaaagcagc ttatgacgcg ggcgcgattt acggtgggtt gattttggtt gcgacatcac
    15181 cgcgttgcgt caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg
    15241 ttggcgtgtt ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc
    15301 tggcggcagt gcaactgcat ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag
    15361 ctctgccagc acacgttgcc atctggaaag cattaagcgt cggtgaaacc ctgcccgccc
    15421 gcgagcttca gcacgttgat aaatatgttt tagacaacgg ccagggtgga agcgggcaac
    15481 gtttcgactg gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct
    15541 taggcgcaga taactgcgtg gaagcggcac aaaccggctg cgccggactt gattttaatt
    15601 ctgctgtaga gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg gttttccaga
    15661 cgctgcgcgc atattaagga aaggaacaat gacaacatta cttaacccct attttggtga
    15721 gtttggcggc atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc
    15781 ttttgtcagc gcgcaaaaag atcctgaatt tcaggcccag ttcaacgacc tactgaaaaa
    15841 ctatgccggg cgtccaaccg cactgaccaa atgccagaac attacggctg gcacgaacac
    15901 cacgctgtat ctgaagcgcg aagatttgct gcacggcggc gcgcataaaa ctaaccaggt
    15961 gctcggtcag gctttactgg cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg
    16021 tgccggtcag catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg
    16081 tatttatatg ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt
    16141 aatgggtgcg gaagtgatcc cggtgcatag cggttccgcg acgctgaaag atgcctgtaa
    16201 cgaggcgctg cgcgactggt ccggtagtta cgaaaccgcg cactatatgc tgggcaccgc
    16261 agctggcccg catccttatc cgaccattgt gcgtgagttt cagcggatga ttggcgaaga
    16321 aaccaaagcg cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt
    16381 tggcggcggt tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg
    16441 cctgattggt gtggagccag gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct
    16501 aaaacatggt cgcgtgggta tctatttcgg tatgaaagcg ccgatgatgc aaaccgaaga
    16561 cgggcagatt gaagaatctt actccatctc cgccggattg gatttcccgt ctgtcggccc
    16621 acaacacgcg tatcttaaca gcactggacg cgctgattac gtgtctatta ccgatgatga
    16681 agcccttgaa gccttcaaga cgctgtgcct acacgaaggg atcatcccgg cgctggaatc
    16741 ctcccacgcc ctggcccatg cgctgaaaat gatgcgcgaa aacccggaaa aagagcagct
    16801 actggtggtt aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt
    16861 gaaagcacga ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag
    16921 cgcaaagaag gcgcattcgt tcctttcgtc acgctcgggg atccgggcat tgagcagtca
    16981 ttgaaaatta tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggcatcccc
    17041 ttctccgacc cactggcaga tggcccgacg attcaaaacg ccactctgcg cgcctttgcg
    17101 gcaggtgtga ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg
    17161 accattccca ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag
    17221 ttttatgccc agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgccaatt
    17281 gaagagtccg cgcccttccg ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc
    17341 tgcccgccaa atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac
    17401 acctatttgc tgtcacgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc
    17461 aatcatctgg ttgcgaagct gaaagagtac aacgctgcac ctccattgca ggggtttggt
    17521 atttccaccc cggatcaggt aaaagcagcg attgatgcag gagctgcggg cgcgatttct
    17581 ggttcagcca ttgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg
    17641 gcactgaaag tttttgtaca accgatgaaa gtggcgacgc gcagttaatc ccccagccgc
    17701 cagttccgct ggcggcattt taactttctt taacgaagcc ggaaaaatcc taaattcatt
    17761 taata
//
LOCUS       DAZRUF010000073        17679 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZRUF010000073 DAZRUF010000000
VERSION     DAZRUF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.073"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(72..1478)
                     /gene="gndA"
                     /locus_tag="ACHXOL_001938"
     CDS             complement(72..1478)
                     /gene="gndA"
                     /locus_tag="ACHXOL_001938"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR6808924.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(1642..3012)
                     /gene="cpsG"
                     /locus_tag="ACHXOL_001939"
                     /gene_synonym="manB"
     CDS             complement(1642..3012)
                     /gene="cpsG"
                     /locus_tag="ACHXOL_001939"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR6808925.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(3117..4553)
                     /gene="cpsB"
                     /locus_tag="ACHXOL_001940"
     CDS             complement(3117..4553)
                     /gene="cpsB"
                     /locus_tag="ACHXOL_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR6808926.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(4556..5779)
                     /gene="wcaI"
                     /locus_tag="ACHXOL_001941"
     CDS             complement(4556..5779)
                     /gene="wcaI"
                     /locus_tag="ACHXOL_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR6808927.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(5776..6255)
                     /gene="gmm"
                     /locus_tag="ACHXOL_001942"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(5776..6255)
                     /gene="gmm"
                     /locus_tag="ACHXOL_001942"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR6808928.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(6258..7223)
                     /gene="fcl"
                     /locus_tag="ACHXOL_001943"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(6258..7223)
                     /gene="fcl"
                     /locus_tag="ACHXOL_001943"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR6808929.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(7226..8347)
                     /gene="gmd"
                     /locus_tag="ACHXOL_001944"
     CDS             complement(7226..8347)
                     /gene="gmd"
                     /locus_tag="ACHXOL_001944"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR6808930.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(8373..8921)
                     /gene="wcaF"
                     /locus_tag="ACHXOL_001945"
     CDS             complement(8373..8921)
                     /gene="wcaF"
                     /locus_tag="ACHXOL_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR6808931.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(8937..9683)
                     /gene="wcaE"
                     /locus_tag="ACHXOL_001946"
     CDS             complement(8937..9683)
                     /gene="wcaE"
                     /locus_tag="ACHXOL_001946"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR6808932.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(9694..10911)
                     /gene="wcaD"
                     /locus_tag="ACHXOL_001947"
     CDS             complement(9694..10911)
                     /gene="wcaD"
                     /locus_tag="ACHXOL_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR6808933.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(10886..12103)
                     /gene="wcaC"
                     /locus_tag="ACHXOL_001948"
     CDS             complement(10886..12103)
                     /gene="wcaC"
                     /locus_tag="ACHXOL_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR6808934.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(12100..12588)
                     /gene="wcaB"
                     /locus_tag="ACHXOL_001949"
     CDS             complement(12100..12588)
                     /gene="wcaB"
                     /locus_tag="ACHXOL_001949"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR6808935.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(12591..13430)
                     /gene="wcaA"
                     /locus_tag="ACHXOL_001950"
     CDS             complement(12591..13430)
                     /gene="wcaA"
                     /locus_tag="ACHXOL_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR6808936.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(13523..15685)
                     /gene="wzc"
                     /locus_tag="ACHXOL_001951"
     CDS             complement(13523..15685)
                     /gene="wzc"
                     /locus_tag="ACHXOL_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR6808937.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(15688..16131)
                     /gene="wzb"
                     /locus_tag="ACHXOL_001952"
     CDS             complement(15688..16131)
                     /gene="wzb"
                     /locus_tag="ACHXOL_001952"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR6808938.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(16137..17276)
                     /gene="wza"
                     /locus_tag="ACHXOL_001953"
     CDS             complement(16137..17276)
                     /gene="wza"
                     /locus_tag="ACHXOL_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR6808939.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
BASE COUNT         4091 a         4770 c         4423 g         4395 t
ORIGIN      
        1 aagataaatg gcaaaaaaaa agcccggtgc aatatacgcc gggcctcaat tttactgttg
       61 gttaaatcag attaatccag ccattcagta tggaacacac cttctttatc aatgcgctta
      121 taagtatgtg caccgaaata gtcacgctgt gcctggatca ggttcgcagg cagaacagcg
      181 gcacggtagc tatcgtaata ggcaaccgca gcggcgaagg tcggaaccgg gataccgttc
      241 tgtactgcat aagcaacgac atcacgcagc gcctgctggt agtcatcggc aatttgcttg
      301 aagtacggag ccagcagcag gttagcgatc tgcggatttt cggcatatgc atcggtgatt
      361 ttctgcagga actgcgcacg gatgatgcag ccagcacgga aaatcttcgc gatttcgccg
      421 tagttcagat cccagttgta ttcttcagac gcagcacgca gctgagagaa gccctgagcg
      481 taagaaacga ttttgcccag atacagcgca cggcgaactt tttcgatgaa ctcagccttg
      541 tcgcctgctg actgagcttg cggaccagag agaactttag atgcggcaac gcgctgatct
      601 ttcagagaag agatataacg tgcaaacaca gactcggtaa tcagcgacag cggttctccg
      661 agatccagcg cgctctggct ggtccattta ccggtacctt tgttagccgc ttcatccagg
      721 atcacatcaa ccaggtagtt accgtcttca tcttttttag tgaagatatc tttggtgatg
      781 tcgatcaggt agctgctcag ttcaccgtta ttccactcgg taaaggtctg tgccagttct
      841 tcgttagaga gattcaggcc gcctttcagg agagaatagg cttcagcaat cagttgcata
      901 tcaccgtatt caataccgtt gtgaaccatc ttcacataat ggcctgcgcc atcagcaccg
      961 atataggtaa cgcacggttc gccatcttca gcaacggcgg cgattttggt caggatcggc
     1021 gcaaccagtt cataggcttc tttctggcca ccaggcatga tggacggccc tttcagcgcc
     1081 ccctcttctc cgccggaaac accggtaccg atgaagttaa agccttcggc agaaagctca
     1141 cggttacgac gaatggtgtc ctggaagaag gtgttaccac catcgatgat gatgtcgcct
     1201 ttatcgagat atggtttgag cgaatcaata gcagcatccg tgcctgcacc tgctttcacc
     1261 attaacagga tgcgacgagg cgtttccaga gattcaacaa actctttcac cgtatagtaa
     1321 ggaaccagtt tcttgcctgg attttcggca atcacttctt ccgtcttttc acgggaacgg
     1381 ttgaaaatag agacggtata accacggctt tcgatgttga gcgcaaggtt gcgccccatc
     1441 actgccatac cgactacgcc gatctgttgc tttgacattg tttactcctg tcagggggtg
     1501 gtcaccgccg agtgtgcggc ttaaatgtgg tatagatgtt aactcactca tagagatggt
     1561 gaatagcatt acctttgcta taccacactg aatatctcac cttttccgtc ttttaaaact
     1621 tatttatatg aaattactga attacttgtt cagtaactca agaataagtt ttgtcttttc
     1681 ttccatcagc ggcacatcac cgcgagattc cacattcaac cgcaccaccg gttcggtgtt
     1741 ggaggtgcgc aggttaaagc gccagtcggc aaaggtcatg ctgatgccat cggtgcgatc
     1801 caccgccagc gcctcgcggc taaaatgctg ttccacgcgg ttaatcgcct caacgggttg
     1861 cgccagtttg ctgttgatct caccgcttgc cggaaacgct gccatccggt cgcgcaccag
     1921 ttcgcccagc gtttttcctt tcaggcacac cagttcggcg accagcagcc acgggatcat
     1981 gccgctgtcg cagtaagcga aatcacggaa gtaatggtgg gcgctcattt caccaccgta
     2041 gatggcgtct tccttgcgca tccgttcttt aataaaggcg tgtccggttt tcgacatcac
     2101 cggcgtgccg cctgcggcag tcaccacatc aacggtgttc caggagagac gcggatcgtg
     2161 gatgatcttc gcgccgggat ttttttcgag gaatgcttct gccagcaggc cgacaatgta
     2221 gtagccctca ataaactgcc ctttttcgtc aaacaggaag cagcggtcaa aatcgccatc
     2281 aaaagcaatg cccatatccg caccgtgttt gatgaccgca ttgcgggtgt cgtcgcggca
     2341 ttccggcagt agcgggttag gaataccgtt ggggaaattg ccgtccggcg tgttgtgcac
     2401 tttgattaat tccacgggtg cgccaagggc tttaaagcgg gcttcgatgg cgtccaccac
     2461 cggacccgct gcgccattcc cggagttaat caccagcttg agcggcgtga ggtttttgac
     2521 attgatataa ccgaacaggt gatcaacgta agcgtcacgc agattgattt gctgatagcg
     2581 accgcgtttg gtttcatcaa cgggaggaaa gtcgttggct tctgccagac gctggacatc
     2641 gcgcagtccg gtatcaccgc tgatcgggcg agccccttcg cgcaccagtt tcatgccgtt
     2701 gtaatccatc gggttatggc tggcggtaac ttcaatgccg ccatccacgc cgagatggaa
     2761 cgtggcgaaa tagatctctt cggtgccgga catgccaata tccagcacat cgacgcccgc
     2821 atcctgtaaa cctctcgcca gcgccagttt taaggtttcg ctggtgaggc ggacatcacc
     2881 gcctaacaca atggttttcg gtttgagaaa ttcgccatag gcgcgaccaa tgcgccaggc
     2941 gatatcttca ttcagttctt cgcctaattt cccgcgaata tcataggctt taaagcaggt
     3001 taattttttc atgtcgttac cctttttcag gcaatagttg gccctgaccg aaacggccaa
     3061 ttttgttttt gtcgttattt acatctcggt ttcgcattca ttgcctgatg cgacgtttac
     3121 acccgtccgt agcgatccgc gaaacgcacc acatcatcct cttcgagata agagccggag
     3181 cgcacttcaa ttaaatcgag cggaattttc cccgggtttt ccaggcaatg catcgccccc
     3241 agcggaatat aaatggactc gttttcacca agcagtttga tatcaccatc aatggtgact
     3301 tttgccgttc ccgcgacaac cacccagtgt tccgcgcggt gatggtgcat ctgtaccgac
     3361 aagccctcgc ccggtttcac ggtgatgcgt ttcacctggt agcggtcgcc cgcgtcgata
     3421 gagtcatatt tgccccacgg gcgatagact tcgcgatgca cccgatgctc atggcgacca
     3481 tcggctttga tctgctcgac cacttttttc acatcctgca ccgcgttacg gtcggcaatc
     3541 agcaccgcat ctttggtctg caccactacc agatctttca ccccgacggt ggttacgagg
     3601 ccagattcgg cgtacacata gctgttttca gttttgtgat taatcacatc gccgtggcaa
     3661 acgttgccct cggcggtgtg ggcgctaatt tcccataatg aggaccagga gccgacatcg
     3721 ctccagcccg catccatcgg caccacaacg gcatctgccg tacgttccat gaccgcgtaa
     3781 tccaccgact cttccgggca ggcgagaaac gcttcttcat ccacacgaat aaaatcgaga
     3841 tccggatcga cggcgctcat cgctttttca caggcatcga gaatatccgg acgatatttt
     3901 ttcagttctt cgagatagcg tccggcgcgg aacaggaaca tgccgctgtt ccagtaatat
     3961 tcgccgcttg ccacataggc ctgggcggtt tccagattcg gtttttcgac aaactgcgcc
     4021 acttcaaagg ccaccgtatc ctgctcaccc gcagacactt caccgcgacg aatatagcca
     4081 taaccggttt ctggtagatc cggcacaatg ccgaaggtca ccagcttgcc cgcttcggca
     4141 tatggcatgg cattacgcac ggcggcacgg aacgcgtctt catcggcaat cacatgatcc
     4201 gccgccaata ccagcattaa cgggtcgctc tccgggctat gacgttttgc cgccagcgcc
     4261 gccagcgcaa tggcaggtgc cgtgtttcgc cctgccggtt cgagaataat gttctcggta
     4321 agtttgttca gttgacgcag ctgttccgcg acaataaagc ggtgctgctc attgcaaatc
     4381 accaccgggc tttcgcactc cacgccgttc aggcggcaga tggtggtttg cagcatggtg
     4441 agatcgcctt tcaggcataa aaactgcttg gggtaaagta cgcgggaaag cggccataag
     4501 cggctaccgg agccacctgc catcacaact ggatagagtt tcgactgcgc cataattatc
     4561 cccgaatatc atttataaat tgacgtaaca cgttctcttt atcgagtgtg cgttcggcat
     4621 attcacgtgc caccgtgttg tgtttgggca gcaggagcgc ctgacggatc cccgccacca
     4681 gcgcctcgac cgattccggt tctacgcaaa ccgcaatgcc cggaaaggtt tcgcaaagct
     4741 gccccagttc tgtgtgggcc tctgcggtaa tcaccgcgtt accgcctacc gccagaatat
     4801 tggtcagttt cgacggcaat acggcatctg ccgcgccgcg tttttgcacc accagatggc
     4861 aatcgcccat cttcagcagt gcgggtaaag cgtcatacga ttgcagcggg aaaaattgca
     4921 tgttgcgcag tccacgctgc tgcgccattt tttccagccg cgctttgccg ccgccctgcc
     4981 cgacaatggc aaaaatcagc ggttcatcgc gcagacgatc ggcagcttca ataacgtttt
     5041 ccagcccctg cttttcacca atattgccgg agtaaagaat gatttttttg ttatccggca
     5101 ggtcaagctg gttacgaagg gcgtcaacat cggcatctgc aacatgctga aaacgggcaa
     5161 tttccgacca gttggggaag aagatgacgt tttccgccgc cacgcctttt tcgatggctt
     5221 tattcatcat cgaacgcgaa atcgtggaga cgttatcgac gttatgcagt ccgctacgtt
     5281 cgaacgccgt tgccagctgt gccactttgc tgcctttgcc ttttccggca aggcccagcc
     5341 ccagcatggc gtccacttcg taatcctgaa tatgcagcac ggtacgcgca ccagaaagtt
     5401 tcgccagcag gcgcattccc ggcgtgcaaa acagcgttgg caccacgccg ataatgcgat
     5461 ccggcttcca gcgacgttgc gccatcagag gaaagaaact gctgacggca aaactgccga
     5521 gatgtaacag gcgtttcagg gtgctcggct gttttggcac gtacagcggg cagcgccaca
     5581 ccgtggcggc cccctcttct cgtttgtacc gccaggcgga atagttctcg cccacctgcc
     5641 actgcgggta gtaaggcggt gcggtaataa cccgcacctc atgaccttgt gccgccagcc
     5701 attccaccat ctcgccggtg tatttgccga taccggttaa ctccggcgag tagttaatgc
     5761 cgtagaccag tattttcata atccgggtac tccggcacgc ttctcagcga gaaaataggc
     5821 gcggctgtta gcgtgaacat cgttactggc gagcagcgcg tccggcgtca gccagcggta
     5881 atcgtcatgc tgctcatccg gcagtatcag ctcttcttca gcgactctga agcgaaaacc
     5941 gagcaccaca tagtgagtgg tgaaatccgt gccggagaag ttatcgtcat aaaagtgctg
     6001 ccagacaccg taaaactggc ctgctgttat cggcagacgc agccccagtt ccgccatcgt
     6061 cagccgctcg aatgcggctt ccagcgtttc gtctttctgc acgcgccctc ccggcacaaa
     6121 ccagtaaccc tgcgccgggc ggttggttct tttgccaagc agaaactcac cgcgactgtt
     6181 ctcgacaata aagtcgagag agacaagcgg ggtggagcgc actaccgtgg caaagtcttc
     6241 ctgacgtaaa aacatcatta cccccgaaag cggtcttgat tctcaaggaa ccactggtaa
     6301 gtgctggcaa gccccgcttc cagtgagatt tcgtgatacc agccaagctg atgcaggcgc
     6361 gtcacatcca gcagtttgcg cggcgtacca tccggtttgc tggcatcaaa aaccacccga
     6421 cctttgtaac ccaccacttt ggcgatggtt tgcgccagct cgcggatagt gcagtcaacg
     6481 cccgtgccga cgttaatgtg cgacagcatc ggctgggtgt tctccagcca gacttcgtgc
     6541 gccagctcca tgacatgaat gctcgccgcc gccatatcat cgacgtgcag gaattcacgc
     6601 atcggtgtac cgctgcccca taccaccacg tccggtgcat tctgtgccgt cgcctcgtgg
     6661 aagcgacgca gcaatgcagg gatcacatgc gaattactcg ggtggaagtt gtcatgcggg
     6721 ccatacaggt tggtcggcat gactgagcgg taatcgcgtc cgtactggcg gttgtatgat
     6781 tcgcacagtt tgatcccggc gattttggca atggcgtaag gctcgttggt cggctccagc
     6841 gtgccctgca ataactcgct ttctgccatc ggctgttttg ccagtttcgg gtagatacag
     6901 gacgatccaa gaaacagcag tttgttcacg tcgttctgat gcgcggcgtg aatgatgttg
     6961 ctctcaatca tcatgttctg gtagatgaaa tccgccggat aggtgttgtt ggcaacaatg
     7021 ccgcccactt tcgccgccgc cagatagacc tggtcaatgc gttcgctggc aaagaaatca
     7081 tgcaccgcgc ggctgtccag caggttcagc tcgtcgcggg tgcgtaatac cagttccaca
     7141 tcaccgcgct gttcgagctg ccgcctgatg gcagaaccga ccatcccacg atggccagcg
     7201 ataaaaatgc gttgtttact catgcttatg actccagcgc gatcgccacg tcgtagccgt
     7261 gagatttcag cagagagtgt tttttcgccg cttcgaggtc attagccacc atttcagaca
     7321 ccatctctct gagggtgatt tccggtttcc agcccagttt ttcgtgcgct ttggtcgggt
     7381 cgccgagcag cgtttcaact tcagccggac ggaagtaacg cggatcaacg gcgataatca
     7441 catcacccgg tttaacgccc ggcgcgtcat gcccggtgac ggaaaccaca atgcccttct
     7501 cttcaacacc cgtgccttca aagcgcagtt tgatgcccag ctgtgccgct gccatttcca
     7561 cgaactgacg cacggagtac tgaacgccgg tcgcgataac gaaatcttcc ggctgttcct
     7621 gttgcagcat catccactgc atttttacgt agtctttggc gtggccccag tcacgcaggg
     7681 aatccatatt gccgaggtac aggcacgact ccagcccctg ggcgatgttg gcgattgcgc
     7741 gggtgatttt gcgggtaacg aaggtttcgc cgcggcgcgg ggattcatgg ttgaagagaa
     7801 ttccgttaca agcgtacatg ccgtaggatt cgcggtagtt aacggtgatc cagtaggcgt
     7861 acagtttggc gaccgcatac ggagatcgcg ggtagaacgg cgtggtttct ttctgcggaa
     7921 tttcctgcac cagaccatac agttcagagg tggaagcctg atagaaacga gttttctttt
     7981 ccagaccgag gaagcggatc gcctccagca ggcgcagcgt acccatcgcg tcgacgtcag
     8041 cggtatattc tggtgactca aaagagaccg caacgtggct cattgcgccc aggttgtaca
     8101 cttcatccgg ctgtacttca cgcaaaatgc gcgtcaggtt agaggtatca ctcaggtcgc
     8161 cataatgcag atggaatttc gggttgcagg tgtgcggatc ctgataaatg tgatccacgc
     8221 gctcggtgtt gaatgacgat gcgcgacgct taataccatg cacctcgtaa cctttttcca
     8281 gcagaaactc tgccaggtaa gaaccgtctt gtccggttac accggtgatg agagcgactt
     8341 ttgacatgtc ttattcctct gtatttttga atttattcag tttcaacgcg ttcgcgtatc
     8401 accactgcgg gattcccccg gcaaaccaca tttgccggaa gcgatttaaa aacactgctt
     8461 cgtgcaccca cgacggtgcc gtcgccgatt gtgacgcctg gggcaacaaa gacatcggtt
     8521 gccagccagc atttctcgcc aatcacaata ggcgtggcgt taatggtgaa atgttgactt
     8581 gcatggtcgt ggctaccggt gcataaataa cttttttgcg atatcaccga atgtgcgcca
     8641 atggttattt caccgagggt atataaattg acgtcatcgc cgacccacgc gtaatcacct
     8701 aaggttaatt tccacggata ggtaattttt actgacggac gaataactac gttttttcct
     8761 atttttgctc cgaataaacg taataaaaaa gcccgccagc gatacaatac ttgtggcgac
     8821 caggcaaata ttgttgcctg tactgcccac cataattgca ctttaatagc gttgccgccc
     8881 cggaaccctt tcggcaccga gaaaccgctt aaatcttgca ttggttttcc ttatattcag
     8941 actttgttat ataaggcttt cgtctttgag gtagtacgtt ggcgtaaatg ccaggataat
     9001 tcagcccaaa agccaggcac atgtaatatt tgtcgttgga cttttttcgc gtcagcacac
     9061 aattccatat tattggtggt agatacccca cccatggaaa attcagacac caggccattg
     9121 agttttttaa atgcataacc ggctttatac attttggctg ccagcgcgta gtcggaagaa
     9181 actttatatt ccaggtcata acgccatttt ttcaagccgg atacagggaa aaatatcgcc
     9241 tgatgactgg cgggcaggct gtgataaata taccagcccg gtttggcgct acgtttaatt
     9301 ttatgcccgt cgccaaaatc cagcagcgca tcgccggtga tcatcatgtt atctttttgc
     9361 atttttaact tacggacaaa atttgcggca tcctgatgaa aaatatcgcc cgaattgaga
     9421 aacaacgcga acttgccttg tgccatcgta atgcctttgt tcatggcgtc gtagataccg
     9481 ttatctggct cgctgacaaa gcgtaggtta tagataccat tgagattttc cagatactca
     9541 cgggtgccgt cattggaacc gccatcgaca acaatccatt cgaagctgat atcttccgcc
     9601 tgcgccagat gcgccagcga ggcatgtgtt ttgactatcc cttcgaggtt acgaaacgcg
     9661 acagtgatta tgctaagcaa catgttctta ttcttacctc gtaatattta acgcttttcg
     9721 caaaataaat ggacagacga ttaaaaatgc atattccggg ctaaatatcg aaccggtaaa
     9781 aaacagcgat accggagtaa aaagatataa ttgcacgcga aaattgcggt tatcaccaaa
     9841 agcgttgatc atcattttta tcactttccc catgtaccac agcgataaaa acactgcgaa
     9901 ccaggaaaaa taaataatca gcagatacaa accattgtct atggtttttc cgacatccgc
     9961 accgttaaat attccgaatg atgcgacgta ttcataaagt gagccaaatc tgactacacc
    10021 gtcaatatgg gtcaaagaat aaccgaccat caccagcgga ccgacaatac gataatacga
    10081 tgacgatcct tccgtaccta aatcgcccag acgggtggaa atataaggaa acgcgattac
    10141 cacaccaacc aggaatacag ccagagaaat caatgccaac ggtaactttt tcttaatcgc
    10201 ctctttattc agatattgaa atgcccactc cagcaaataa aacaggataa aagtcataac
    10261 ccctgaaaac gatccggata atattatccc tgcgagaatc atagcatcgg ttttaggcgt
    10321 tttgatacca aactgtttga tgctgagcca aattgagatt aatgccagag cgaaaaatgc
    10381 cggttcgaaa taaagtgctg tcgtgcgctt gccgccgaat ttaatgaaat tcagtacata
    10441 gctgttgctg taaatcagat atttcgaaat cgactccata atactgctgc caccggtgag
    10501 gataatttgc gccatctcca ccgccgccag cgccaccacc agcccgatca ccagataaaa
    10561 gaaacgtaat atcttgcgat ggttgtgtgg cgaaatcgtt ttaaagcgaa tactccacac
    10621 catgccaata atgatcacaa tatagacaaa cagcatggtt gaagtgacgt atttactggc
    10681 atccagcgac tgaccaaaca gatagttaaa cgccgtgagt cccgcaccaa tccctaaggc
    10741 aatcatcaat tttttaacgc tgatgcgttc taaaaacagc agtaacaaga caggtaaaaa
    10801 agtgacgatg gtgatgggga aactttcgcc aagctgggcg attttgacgt taaccagcag
    10861 gtagatcagc ggcagcagca ggtagctaca gattctgata gaagttgaca tactcctcca
    10921 gcatctgttg tccactgtag gtggcgcggc tgcgttggct gaactcagcc agcgtggtac
    10981 caaatatcgc ctgcgcgatt tccggtttgc ttaactgcac cagttgcagc acgtcttctt
    11041 cgtcgacggt tttaccgccg gatttttgca acacttcccg tgccgcgtcg ctatgggtgg
    11101 caatcaccgg cacgccaatt gataacgcct cacacaaaat cagcgggtag ttatcgacgc
    11161 gagaactgaa taccagcgca tccatctgat tgagcgcgct catcaacttg cgcttgtcag
    11221 tttcaaagcc gtgattaacc acgttgccag cggtgaacgg cgagaactta ccaaaggtat
    11281 gcagttcgat tttgtcgccc agcgccatca tctcacgcac cagttgctgg ttagtcttgc
    11341 cgtcgtaacg caagtcatgc gccaccaccg cgattttcgg cttgccctgg gtttcgcgca
    11401 ccggaggcaa gtccgccaga atcgcttcgg ttgccatatc aataccatta ttgataatcc
    11461 ggcaacgccc tggaccgtac aggctattga aagcgtcagc cacatgctgg ctgggggaaa
    11521 taaactgaca gcccagcgcc agcatctcac ggaataactg gcgtttgccc gccaccagct
    11581 ggtgtgcgcg atcaatcttc accggcggat agttatttaa ggtcgggcat ttctggcagc
    11641 ccgttttcca gccttcgcaa ccgtcggtaa aggcgcagcg tccggtaaca ctccagtggt
    11701 cgtgcagcgt ccagaccaga gtgacgtccg gtttatggtt tttcactttt tcgcaaaagc
    11761 gcaccacgct cttaagattt agccagtagc tgtgcagcac atgaaaatgc aggaccaccg
    11821 gacccggtgt gcgagtaatg gtgcgatata actcattgaa attgccaaac agatcgcgat
    11881 taaacagacg aaacagggca atattcgcca tcgcggtcat ccgcggcgta tgtttgatga
    11941 cctgcggata gttctgatgg ctgacgctct ctttgccgcc tttgccgtaa ccgtagacaa
    12001 aatgtgacgc cagcccctgt tgcagcgcac gctggtggag atctaacgcc acacctgctg
    12061 ccccgccttc cgccagtcgc acattaaatt gcagaatatt catttaatta ccttcactcg
    12121 cgctttttct cccaccacca gcgcgttgtc cgggacagag tcgagcacca cgctgcccgc
    12181 acccacggtg acgttgttac caagcgtgat atcaccaaga ataatgacgt tggcaccgag
    12241 ttcgacgccg ttgccaatgt gtggacatgc catgttatcg gcaccacgat tgccgatagt
    12301 gacgccgtgg cgaatggtga aatcatcccc cgctaccacg tttttattga tcactacggc
    12361 gtaaccgtga tggatagtaa agcggcggcc aatggtcgcg gcggcctgga tttcataacc
    12421 gaaaaagcat tcggtgataa tgcgatacag caccagcagc ggggccgccc acagattgtt
    12481 gaggacgttc tttttgcgcc acaccgaaca aaaatgagca acacgatagg caagaaccat
    12541 gcagcacggg cgtaaactcc agctgttggc acgcagatct tccagcatct ttagcgcccc
    12601 cgaataccat cagccagacg tttgccgtta cgcaccgaca gcagcgtcag caaagtgcgc
    12661 caggtcatac gtttattgcg gatctggtag agggtaaaca gctgatattt tttgcttgca
    12721 cggtcaaatt tatctttgtg ctttcggtaa aagtggaagt acccggagaa ttttttcggt
    12781 gacgaggtga tctgcatttc accgtgattg atatgcagga tctgcgtcgc ttcttccact
    12841 ttccacggtt cgccgtactc caccaccatc cgcaggaaga tgtcgtaatc ctgtgctgct
    12901 ttcagttcgg tatcgaacaa acactctttg aaacgccatg cccaagtaaa gacctgattg
    12961 ccaatgatat tgcgtttata gaacaggcgg cgtgagtacg gcgatttggg atacagtggt
    13021 aagcttgccg gttgggaata aacttctccc tggcatacgt agtcgttagc gtacaaaaag
    13081 gcgtgagtga ccagttgctg tttatgggcg aggaagacgc tcagacggtt gggtgtccat
    13141 tcgtcatcgt catcgatccc ggtaatgtat tccccttgcg ccagcataat cgcctggtta
    13201 cgtaccgcgc acgccccgct gttaatgtcg ttgtgaatgt aggtgatacg cggatcgttg
    13261 agggcagtga cgtactgttg cagttgttcc caggaggtgg agcaatcatc cacgatgatc
    13321 atctcccagt tgctgtagtc ctggcgcagg accgatttta tcgcccgaat cgccagttgt
    13381 tggcggttcc aggtcggcat atagattgaa atcagcggat tctctttcat aggttgttct
    13441 cccggcttat gagctggttt gcttgccgga tgcggcgtga gcgccttatc cggcctacgg
    13501 ggcggtgcga atgcaggcgt cgttatttcg catccgactt atattcgtat tcgtaatagc
    13561 cataatcctg atacgcgctg gcgcggcgga agatggagtt cagaatcacc cctttcaccg
    13621 gaataccgtt ttgctcaaag cggctcagac tggtttccac ttctttcaat gtgttgaccg
    13681 catagcgcgc caccattaac gtggttccag cgtgacgccc gacaattgct gcatcggtca
    13741 ctgccagaat cggcggcgta tcaatcaaca ccaggtcgta gtttttactc gcccagctca
    13801 ccaattcggc aaagcgttcg ctcatcaaca gttcagaagg atttggcggc acctgaccgc
    13861 gcgggatcag gtcaaatttg gcaatagagg tcggtttagc tgcggtagta atatcgccct
    13921 ggccaatcag aatttccgac aggccattga cgttattggt acccaacagc tcgtgggtgt
    13981 agcctttgcg catatcgcag tcgatcaaca acacccgttt attggtctga ctgatcaccg
    14041 ccgccaggtt ggcgcagaca aaggttttac cgattgacgg gctcacccct gtcatcatca
    14101 acacattgtt ctgcgcctgc atcatcgcga agtgcagact ggtacgcaga ctgcggatgg
    14161 cttcaatcgc cagatcggtt ggattcccca ccgccagtag ctggctctgt ttatagcgtt
    14221 taatcccttt gatggttttg acgctatcgc gcgctttctg ccattccgac agcgggatgc
    14281 tggcatagac gctgatacca tgttcttcca gcacctgcgg gctttcgatg ccgcgattaa
    14341 acaacgagcg cagcagcaca cccacgatag agagcatcag gccgaggata attgcgccga
    14401 ggataatcag tcctttcttc ggtttcagca cgccaggctg ggtgattgcc gggtcaacaa
    14461 tgcgcacatc gccgacggtg ctggcttcgg tgattttcag ctcctgctct ttattcagca
    14521 gttgcatata gacctgctga ccagactcga catcgcgggt caggcgcaca atctcctgct
    14581 gggttttcgg catcgccgtt acgcgaccgt taagtttggc tttctcttct tccagcgcct
    14641 gacgtttctc cagcagcgtg cggtacgccg ggtgaacttt ggtgtacagc ttggagattt
    14701 ccgcctcttt aaaggtcagt tcgttcaact gcgcgtcgat gttcaccatc gaatcgagca
    14761 ccgctttcgc ttccagcggc agatcaacag aatctttatc ctgacggaag gcattcagtt
    14821 tgttttcggc aacatcgagg cggctacgta cttccggtaa ctgttgcgcg aggaaagcga
    14881 ggcttttcga cgcttccgcc gatttgcgct caatattttg ttcctgatag ttacgggcga
    14941 tgctgttaag aatgtcgcgg atctgttcgc gatcttcacc ggtataagtc aggctcagta
    15001 cgcctgcgtc tttgccgttc tccgttaccg tcaggctgtt ttgcagttgg ttgatcatcc
    15061 ccaacgtgga gtatttggtg acggtaaact cactgcccgg gcgggcgtga atggcttcaa
    15121 ccatcagcgt gacgccttct tttttcagca tctggcccgc ttgcccacgg gcgctaaagc
    15181 cgccatcact gctcagggtg tagtttttat tgtccagcac attaagcgta aacacctggc
    15241 ccgccatctc tttcgggcgg ttaaaggtag tcactttcac cgtctcgttc tggcgtccca
    15301 tcagtcgctc ccagcccgcc ccgaaaatcg ggaacgtgtt tttgctcact gcaatatcga
    15361 ggtcgagatc gtccaccgtt ttaccaagca ccagacgcga acgaatcaac tggatctcgg
    15421 cgtccgatgc aggcggtttg ttggctaatg ccgaaccaat gtcctgcact aacgaattgc
    15481 cgctgttttg ctcgatttgt accagtgcgt cggcactata aatcggcgtt gcgaagaagg
    15541 tgtaaaccac ggcacagagg gcaaatacag cggtgatgcc aatcacccac cagcgcgctt
    15601 caatgacggt gccgaccagg cgaccaatat cgatttcatc actgcccgtt accggagcgg
    15661 catgttgttt tactttttct gtcattctta tacctgctct gcgttcaatg cctgcgccca
    15721 ctggcgggca gaccgttcaa gtaatgtgta caccgctgca aacgtttccc ggcttttgcg
    15781 atacggatcg gggatttcac attcgttatc ccagtgacca aacagcatca ctttgccgcg
    15841 catctcaggt gccatctcgc ataagcgttc gatatggcgc ttttccatgg tcaaaatcag
    15901 gtcatagttg cgacacaggc ggcggctgat ttgtcgtgca cagtggcctt ccagagacag
    15961 ttgatgttct gcggcgacgc tgatagcggt aggatcagcg cccttaccga ccagcgcgcc
    16021 gagtccagcg gactccactt tcagttccgg gtgataacgt tgcagtaagc gttccgccgt
    16081 cggggaacgg caaatattgc cgacacagac aactaatatg ttgttaaaca tgacgattac
    16141 cagttatgaa tgtcgctggc tgtatccgtc atgtaacgga caccgctaat agttggcaac
    16201 aactgattga tcagacggtt ccagcgggaa accggggcgg tggtgacata caccacgtca
    16261 taaggttgca ggcggaattc tgtcgccatc accagcgacg tggcatcgga catatccagc
    16321 tggtagatat tggcaatctt gccattacgc ccgccctcgc ctttcagcgg acgaatgaca
    16381 aagatgccgc tggcgttgga ggtggtcatg tcgatacctt cagcattacc cagggcttca
    16441 gtcagggtca tgccgctaaa gtccattttc agggtgctct gtttcttcac ttcacccatc
    16501 acaaatactt tcagatcatc attacgcggc acgtagagaa tatcgccggg ataaagcagt
    16561 cggttctgat tgaggtcgcc gttttgcatc agcgcctgca aagaaatgcg ttcttcacga
    16621 ccattgtgtg ttagcactac gttgcgccag tcagcggtgt cggtcaggcc acctgcggcg
    16681 ttgatggcgt cgagaatagt cagtggcacg ttggtgatcg cctgttgacc ggatttattc
    16741 acctgaccgg agatataggc cttttgtgag cggaaggcgg cgatattaac gtccacctgc
    16801 gggtcagcga tgtacgtcgc taagcgcccg gtaatatcac tgcggatttc agcgagcgtt
    16861 ttcccgacta cgtgaacctt gccgatatac gggtaaaaca tagtgccgtc aggctgtacc
    16921 cagttgccgg tgtcgctgga gctgcggtac tgaccggctg gcgtggtgag ttccgggtga
    16981 tcccagacgg tgacattcag aacgtcgccc ggcccgacgc gatactggta attcgcgatc
    17041 tcgctttcca gcgtcatatt ggggcgcgct acattcgggc gtgggcgtaa ttggtcaatc
    17101 aggcgcgggg tcagcggata aacattcacc attttgtcga gatcgaaatc agcgtcctgc
    17161 tgtttgatga cgtctttgcc catcgtcgac atattgctgc ccggaagtac tgtgcaaccg
    17221 cttatcaagg ttactgacac caataatggc atcaatttca ttttggattt catcattgtt
    17281 tatttatcac tttggcagag taattatcct gtgcactatt aatagcaata tcgccatgca
    17341 catttacctt gcagttaatt gaataaaaat ttaactggca tcagtcctaa aaaaattgat
    17401 ttcatccgca ggctattgac agaataattc agactggtct ttcaggcatc cagacacgct
    17461 accgcccctg gctttttagc caccaataca ctgatttagc ttaatttttc aaaccctctc
    17521 tgcatacaat cgttgatgag aaagggttat tgcggaaatt aacttccgaa tataaggtga
    17581 cattatggta attgaatatt ggctctccaa taatgcagga ggaagtgtta cagctaacgg
    17641 aatagcaggc aaggtaacaa ttcggcaatt ggctatttt
//
LOCUS       DAZRUF010000074        17451 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZRUF010000074 DAZRUF010000000
VERSION     DAZRUF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17451
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.074"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            15..1091
                     /locus_tag="ACHXOL_001954"
     CDS             15..1091
                     /locus_tag="ACHXOL_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR6808940.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="ACHXOL_001955"
     CDS             complement(1484..1825)
                     /locus_tag="ACHXOL_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR6808941.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHXOL_001956"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHXOL_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR6808942.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHXOL_001957"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHXOL_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR6808943.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="ACHXOL_001958"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="ACHXOL_001958"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR6808944.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /gene="yobB"
                     /locus_tag="ACHXOL_001959"
     CDS             3559..4215
                     /gene="yobB"
                     /locus_tag="ACHXOL_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR6808945.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="ACHXOL_001960"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="ACHXOL_001960"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR6808946.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHXOL_001961"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHXOL_001961"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR6808947.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="ACHXOL_001962"
     CDS             complement(7167..7826)
                     /locus_tag="ACHXOL_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR6808948.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHXOL_001963"
     CDS             complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHXOL_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR6808949.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHXOL_001964"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHXOL_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR6808950.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="ACHXOL_001965"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="ACHXOL_001965"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR6808951.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHXOL_001966"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHXOL_001966"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR6808952.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHXOL_001967"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHXOL_001967"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR6808953.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHXOL_001968"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHXOL_001968"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR6808954.1"
                     /translation="MAVTQTTQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="ACHXOL_001969"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="ACHXOL_001969"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR6808955.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="ACHXOL_001970"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="ACHXOL_001970"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR6808956.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..>17451)
                     /locus_tag="ACHXOL_001971"
     CDS             complement(17270..>17451)
                     /locus_tag="ACHXOL_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808957.1"
                     /translation="SIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGY
                     PCIHFTGGCDYDWIE"
BASE COUNT         4415 a         4410 c         4279 g         4347 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggttgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa t
//
LOCUS       DAZRUF010000075        16701 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZRUF010000075 DAZRUF010000000
VERSION     DAZRUF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.075"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..505
                     /locus_tag="ACHXOL_001972"
     CDS             <1..505
                     /locus_tag="ACHXOL_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFR6808958.1"
                     /translation="LAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEM
                     QRKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCA
                     LVVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGI
                     MPSLPFL"
     gene            complement(561..3416)
                     /gene="valS"
                     /locus_tag="ACHXOL_001973"
     CDS             complement(561..3416)
                     /gene="valS"
                     /locus_tag="ACHXOL_001973"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR6808959.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(3416..3859)
                     /gene="holC"
                     /locus_tag="ACHXOL_001974"
     CDS             complement(3416..3859)
                     /gene="holC"
                     /locus_tag="ACHXOL_001974"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR6808960.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(3993..5504)
                     /gene="pepA"
                     /locus_tag="ACHXOL_001975"
     CDS             complement(3993..5504)
                     /gene="pepA"
                     /locus_tag="ACHXOL_001975"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR6808961.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5658..5708
                     /gene="ytgA"
                     /locus_tag="ACHXOL_001976"
     CDS             5658..5708
                     /gene="ytgA"
                     /locus_tag="ACHXOL_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR6808962.1"
                     /translation="MSIIILATISTTQENK"
     gene            5771..6871
                     /gene="lptF"
                     /locus_tag="ACHXOL_001977"
     CDS             5771..6871
                     /gene="lptF"
                     /locus_tag="ACHXOL_001977"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR6808963.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6871..7953
                     /gene="lptG"
                     /locus_tag="ACHXOL_001978"
     CDS             6871..7953
                     /gene="lptG"
                     /locus_tag="ACHXOL_001978"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR6808964.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(8072..9574)
                     /locus_tag="ACHXOL_001979"
     CDS             complement(8072..9574)
                     /locus_tag="ACHXOL_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR6808965.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(9652..10650)
                     /gene="idnR"
                     /locus_tag="ACHXOL_001980"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(9652..10650)
                     /gene="idnR"
                     /locus_tag="ACHXOL_001980"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR6808966.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(10717..12036)
                     /gene="idnT"
                     /locus_tag="ACHXOL_001981"
     CDS             complement(10717..12036)
                     /gene="idnT"
                     /locus_tag="ACHXOL_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR6808967.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(12113..12877)
                     /gene="idnO"
                     /locus_tag="ACHXOL_001982"
     CDS             complement(12113..12877)
                     /gene="idnO"
                     /locus_tag="ACHXOL_001982"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR6808968.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(12901..13932)
                     /gene="idnD"
                     /locus_tag="ACHXOL_001983"
     CDS             complement(12901..13932)
                     /gene="idnD"
                     /locus_tag="ACHXOL_001983"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR6808969.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            14149..14712
                     /gene="idnK"
                     /locus_tag="ACHXOL_001984"
     CDS             14149..14712
                     /gene="idnK"
                     /locus_tag="ACHXOL_001984"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR6808970.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            complement(14716..15735)
                     /gene="ahr"
                     /locus_tag="ACHXOL_001985"
     CDS             complement(14716..15735)
                     /gene="ahr"
                     /locus_tag="ACHXOL_001985"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR6808971.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            15931..16015
                     /locus_tag="ACHXOL_001986"
     tRNA            15931..16015
                     /locus_tag="ACHXOL_001986"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15965..15967,aa:Leu,seq:caa)
     gene            16201..>16701
                     /locus_tag="ACHXOL_001987"
     CDS             16201..>16701
                     /locus_tag="ACHXOL_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR6808972.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQR"
BASE COUNT         3990 a         4349 c         4181 g         4181 t
ORIGIN      
        1 tctcgcattt ttggctttat taccttttct cgctgttgct ggttatatta tcttcgatca
       61 aatattaaat gagtatgata gttctgggta tgcaaatgat gatattgaga atttacagca
      121 atttatggaa atgcaacgta aaatgataat cgcgcagtta atctattatt ttgggattgc
      181 tgttagcacc agttatttaa cggtgtcgtt gcgaaatcat tttatgagca acctgtcact
      241 gaatgatggg cgtattcgtt ttcgctcaac tttaacatac cacggtatgc tttatcgcat
      301 gtgtgcgttg gtggtgatat ccgggattac gggcggtctg gcttatccac tgctgaaaat
      361 atggatgatt gactggcagg caaaaaatac gtatttgctg ggcgatttgg atgaccttcc
      421 tttaatcaat aaagaagaac aaccagataa aggattctta gccagtattt cacggggaat
      481 tatgccttct ttaccatttc tgtaagagat aaaaaaggcc ggagcatgct ccggccttcg
      541 ttttcatcac tgtgttttga ttacagcgcg gcgataacag cctgctgttc aatcagtttc
      601 gctttcgctt ccgcatagcc ttccagcttc tcacgctctt tcgcgatgac cgcttccggt
      661 gcgcgggcga caaagccttc attcgccagc ttgttctcga tacggctgat ttcgccttcg
      721 atcttcgcca cttctttcgc cagacgcgcc agctcatctt ctttgttgat gaggccagcc
      781 atcgggatca gcagctctgc gccgtcgatg atcttcgtaa cggaaaccgg acctttgtca
      841 tcggcaggca gcacggtgat gctttccaga cgcgccaggg tttgcaggaa gccacggttt
      901 tcatttacgc gacgttctgc atccgcgctg caaccacgca gcagcagctc cagcggtttg
      961 cccggcgcga tattcatttc tgcacggatg ttacgtaccg caacgatcgc ctgtttcagc
     1021 cattcggtgt cggccagtgc ggcttcatca acctgagatg catcgtactg cgggaacggc
     1081 tgcagcatga tggtgtcggc agtgataccg caaagtactt tcacacgctg ccagatggtt
     1141 tcggtgatga acggaatgat cggatgcgcg aggcgcagca gaccttccag tacggtcacc
     1201 agcgtatggc gagtaccgcg cagttcggct tcggtgccac cgttcattac cggcttagtc
     1261 agctccagat accagtcaca gaactggttc caggtgaact catacagaat gcctgcagcg
     1321 atatcgaagc ggaagctgtc cagcgcttcg cggtacgctt tgatggtctg gttgaactct
     1381 gccagaatcc agcggtccgc cagcgacagc gtcatttcac caccgttgaa gccgcaatcc
     1441 tgaccttccg tgttcatcag cacaaagcgg ctggcgttcc acagcttgtt acagaagtta
     1501 cggtaacctt ccaggcgctt catatcccag ttgatgtcac gaccggtaga cgccagcgcc
     1561 gccagggtga agcgcagggc atcggtgccg tgcggctcaa taccgttcgg gaactgcttc
     1621 tcggtgcgct tacggatttt gtccgccagc tgcggctgca tcatattgcc ggtacgtttc
     1681 tccagcagtt ccggcagcga aataccgtca accatatcca gcgggtcaat aacgttaccc
     1741 ttggatttgg acatcttctg gccttcgtcg tcacgaatca ggccggtcat gtaaacggtg
     1801 tggaacggca cctgcggttt gccattttca tctttgatga agtgcatggt catcatgatc
     1861 atgcgggcaa tccagaagaa aatgatgtcg aagccggata ccatcacgct ggttgggtgg
     1921 aactggcgca gtgcgtcagt gttttccggc cagcctaggg tagagaaggt ccacagcgca
     1981 gaagagaacc aggtgtcgag aacgtcttcg tcctgacgca gggcaacgtc ggcaccgagg
     2041 ttattttcct tgcgcacttc ttcttcgttg cggccaacat aaacgttacc tgcttcgtcg
     2101 taccatgcag ggatacggtg accccaccac agctgacgag agatacacca gtcctgaata
     2161 tcgcgcatcc aggagaagta catgttttcg tactgcttcg gtacgaactg aatgtcgccg
     2221 ttctcaaccg cttcaaccgc cggtttcgcc agtacatcgg cacgcacgta ccactggtcg
     2281 gtcagcattg gttcgataac tacgccgcca cggtcgccgt aaggaacggt caggtcgtgc
     2341 ggtttaattt cttccagcag gccgagtgcg tcaaccgcag caacgactgc tttacgtgca
     2401 gcaaaacgct cgagtttctg gaactctgcc gggatttcgc tggaataaac gtcagattcg
     2461 ttacctttgg tatcgaacac ctgggcgctt tcacggatat cgccgtcaaa ggtcaggatg
     2521 ttgatcatcg gcagcgcgtg acgtttaccc acttcatagt cgttaaagtc gtgcgccgga
     2581 gtgattttca cgcagccggt gcctttttcc atgtcggcgt gttcgtcgcc aacgatcgga
     2641 atacgacggt taaccagcgg tagaatgaca tatttgccaa tcaggtcttt gtaacgcgga
     2701 tcttccgggt taacggccac gccggtatcg cccaacaggg tttccggacg ggtagtcgca
     2761 accaccagat aatctttacc gtctgcggtt ttcgcgccgt cagccagcgg atagcggatg
     2821 tgccacatcg aacctttcga ttcgcggttt tccacttcca ggtcagagat agcggtgcgc
     2881 agtttcggat cccagtttac caggcgtttg ccacggtaaa tcaggtcttc tttatacaga
     2941 cgaacgaaaa cttctttcac cgcattggac aggccttcgt ccatcgtgaa gcgttcacgc
     3001 tcccagtcga cagagttgcc gagacggcgc atctgacggg taatggtgcc gccggactcc
     3061 gctttccatt cccagatttt gtcgatgaaa gcttcgcggc cgtagtcgtg acgggtttta
     3121 ccttcttctg cggcaatctt gcgctcaacg accatctggg tagcgatccc ggcgtggtca
     3181 gtaccgacct gccatagggt gtttttgccc tgcatgcgct gatagcggat catggtatcc
     3241 atgatggttt gctggaaggc gtgacccata tgcaaactgc cggtgacgtt cggcggcggg
     3301 atcatgatgc agaaactttc ctggctttca tcgccattag gcttaaagta gccctgcttt
     3361 tcccagtgct cgtaaagcgg ctgttcgata tcttgtgggt tatatgtctt ttccattatt
     3421 tccaggttgc cgtattcagg ttgaaaccag ccacgcggta ggctttatag cgttcgcgcg
     3481 ccagttgttt cagagaatct tcataaggaa cgaagtctac cacttctgtg aaagcagtgg
     3541 caaaatctgc aaagcttgtt cgcagactaa tcaatatatc gcgcgggctg ctgctgcgtt
     3601 tttgcggcca ggcgatctcc accggcgcac cgccgcgcgg tccttctccc gctaaattat
     3661 gcgggacaaa gctttctgcc ggacgcgccc acagggcttc atccagccgg taagcctgct
     3721 tttcatcttc acaggcgatg agcacgcgct taccgctgcg ccaacgttct gcggcaattt
     3781 cacacaccag ttgctcaacg gcgcttaagc catcgacggt ggtgtcattg tccagaaggt
     3841 agaacgttgc gtttttcata tatggggctt cttgtggtgg atttaaatat tgctgcattc
     3901 ctcaccctga aagatgagca atgaatttct attgcgctcg atcggttccc tctcccttag
     3961 ggagagggtt agggtgaggg taaaacgcaa acttactctt cgccgttaaa cccagcgcgg
     4021 tttaacagga actgtgccag caacgctacc ggacgaccgg ttgcgccttt tgctttacca
     4081 gaacgccagg cggttcctgc aatatccagg tgcgcccagt tgtacttacg ggtaaagcgt
     4141 gacaggaagc aacctgcggt aatcgcccca ccaggacgac cgccaatgtt cgccatatcg
     4201 gcaaaattgg actccagttg ttcctgatac tcgtcaccca gcggtaagcg ccatgcgcgg
     4261 tcaccggatt gttcagacgc ggcaatcagt tcatgggcca gcggattatg gttcgccatc
     4321 agaccagtaa tatgatgacc cagcgcgatc acgcaggcac cggtcagcgt cgccacatca
     4381 atcaccgctt ccggctcaaa acgttcaacg taagttaaca cgtcgcacag taccaggcgg
     4441 ccttcggcat cggtgttcag cacttcaacg gtttgaccgg acatggtggt taacacatcg
     4501 cccggacgat aggcacgccc gccaggcatg ttttcacagc ctgccagcac gccaataacg
     4561 ttaataggca gttgtagctc tgcgaccatc cgcatcacgc cgtaaaccgc tgccgcaccg
     4621 cacatatcgt acttcatctc atccatgcct tctgaaggct tgatggagat accgccggag
     4681 tcgaaggtta aacctttacc caccagcacg attgggcgtg catcttccga cgcgttgcct
     4741 ttgtactcaa tcaccgacat cagcgattcg ttttgcgaac cctgaccgac cgccagataa
     4801 gagtgcatcc ccagctcttt catctgctgt tcgccgataa cgcgggtgat aacattcttg
     4861 ctgtagctgt cagccagctg gcgcgcttgt gaagcgaggt aagcggcgtt acagatattc
     4921 ggcggcatat tgccgagatc ttttgctgct ttaatcccgg cggcaatcgc cagaccgtgc
     4981 tggatcgcgc gctcaccgct ggtcagttca cggcgggtcg gcacgttgaa caccatttta
     5041 cgcagcggac gacgcggttc gctcttgttc gttttcagct gatcgaaact gtagagcgtc
     5101 tcttttgcag tctcgacagc ctgacgcact ttccagtagt tgttacggcc tttaacgtgc
     5161 agttcagtca gaaagcagac cgcttccatt gagccagtat cattcagcgt attaatggtt
     5221 ttctgaataa cctgcttgta ctgacgttca tccagctcac gttctttgcc gcaaccaata
     5281 aggagaattc gctcggaaag tacattcgga acatggtgca gcaacagtgt ctgccccggt
     5341 tttccttcca gttcgccccg acgtagcagg gcgctgatgt acccatcgct gattttatcg
     5401 agctgttctg caatcggaga aaggcgacgt ggttcgaaga cgcccacgac gatgcaggca
     5461 ctccgctgtt tctccgggct accgcttttt acactaaact ccatgcacta cgctcctgaa
     5521 tcttaaagac aacggcggtg gctacagata gaattgcaag ctttcgtaac tcatgtccgc
     5581 tgttgcgatg acttcgtgtt aatcttaacg ttattacggc attggcacgt cagaacaaaa
     5641 tctgagaggt gaatccgttg agtataatta tcttagcgac gatttcgacg actcaagaga
     5701 ataaatgacg tttaagccat gaaacaagct aaaatcctgc aaaagacgag tttttacggg
     5761 cgtatttaaa gtgataatca taagatatct ggtgcgggag acgctcaaaa gccagctggc
     5821 gatactcttc atcttgcttt tgatcttctt ctgtcaaaag ttagtgagga tcctcggcgc
     5881 agcggttgac ggcgatattc cggcgaatct ggtgctctcc cttctcgggt tgggcgtgcc
     5941 ggaaatggcg cagcttatcc tgccattaag cctgttcctc gggctgctga tgacgctggg
     6001 caaactgtat accgaaagtg aaattacggt aatgcatgcc tgcggtctga gcaaagcggt
     6061 gctggtgaaa gcggcaatga tccttgcggt gttcacggca atcgtcgcgg cggttaacgt
     6121 gatgtgggcg ggaccatggt catcgcgtca tcaggatgaa gtgttagcag aagcgaaagc
     6181 gaaccctggc atggcggcgc tggcgcaagg gcaattccag caagcgacta atggcagctc
     6241 ggtgctgttc atcgaaagcg ttgacggcag cgatttcaaa gatgtgttcc tcgcacaaat
     6301 tcgaccaaaa ggtaatgcac gtccttctgt ggtggtggcc gattccggac atttaaccca
     6361 gctgcgcgac ggctcccagg tcgtcactct caaccaggga acgcgcttcg aaggcactgc
     6421 actgttacgc gatttccgca ttacggactt tcaggattat caggcgatca ttggtcacca
     6481 ggcggtggtg ctcgacccga acgataccga ccagatggac atgcgcacat tgtggaacac
     6541 tgacaccgat cgtgcccgcg cagaactgaa ctggcgtatc acgttggtat tcaccgtgtt
     6601 tatgatggca cttatggttg taccgttgag cgtggttaac ccgcgtcagg gacgcgtact
     6661 gtcgatgctg ccagccatgc tgctgtatct acttttcttc ctgatccaga cctccctgaa
     6721 atcgaacggc ggtaaaggta agctggaccc gacgctgtgg atgtggaccg ttaacctgat
     6781 ttatctggct ttggcgattg ttctcaacct ttgggacacc gtgccggtcc gccgcctgcg
     6841 cgccagtttt tcgcgtaaag gagcggtgtg atgcaacctt ttggcgtact tgaccgctat
     6901 atcggtaaaa ctattttcac caccatcatg atgacgctgt tcatgctggt gtcgctgtcg
     6961 ggcattatca agtttgtcga tcagctgaaa aaagccgggc aggggagtta cgacgcgtta
     7021 ggcgcaggaa tgtatacctt gctgagcgtg ccgaaagatg tgcaaatctt cttcccgatg
     7081 gcggctctgc ttggggcgtt gcttggtctt gggatgctgg cgcagcgcag cgaactggtg
     7141 gtgatgcagg cttctggttt tacccgtatg caggtggcgc tgtcggtgat gaaaaccgcc
     7201 attccgctgg tcttgctgac gatggcgatt ggtgaatggg tcgcgtcgca gggcgagcag
     7261 atggcgcgta actaccgtgc gcaggcgatg tacggcggct cgttgctctc cactcagcaa
     7321 ggcttatggg cgaaagatgg caacaacttc gtctacattg agcgggttaa aggtgacgaa
     7381 gagttaggtg gcatcagtat ttatgccttt aacgagaatc gtcgtctgca atccgtacgt
     7441 tatgccgcta ccgcgaagtt tgacccggaa cataaagtct ggcgtctgtc gcaggttgat
     7501 gaatctgatc tgaccaatcc gaaacagatc accggttcgc agacggtgag cggcacctgg
     7561 aaaaccaacc tcacgccaga caaactgggc gtggtggcgc tggacccgga tgcactctcc
     7621 atcagtggtt tgcacaacta tgtgaagtat ctgaagtcga gcggtcagga tgccggacgt
     7681 tatcagctca acatgtggag caaaatcttc cagccgctat ccgtggcggt gatgatgttg
     7741 atggcgctgt cgttcatctt tggcccactg cgtagcgtac cgatgggcgt gcgtgtggtc
     7801 accggtatca gcttcggttt tgtcttctac gtactggacc agatcttcgg cccgctgacg
     7861 ttggtttatg gcatcccgcc gatcatcggc gcactgttgc caagcgccag cttcttctta
     7921 atcagcctgt ggctgttaat gagaaaatcg taatctaaca agggagcgaa agctcccttt
     7981 attattgttg acaaagtcaa atacgagttc gaacggtccc ctcgcccctt tggggagagg
     8041 gttagggtga ggggcacaga tcacgtctga cttacctttt tctccccccc agcaaactcc
     8101 ccaacatccc gcgtacaatc tgattcgtca cctggcgagc ggcgcttttc gccattgttt
     8161 gcaccacacc atctttcttc ccgccgcgtg gtccggtagt gccaaacaaa atatccttca
     8221 atccaccaag aataccgtca tccaccgcca cctctttccc tttcacgggg ggattatttt
     8281 gctgctcggt actggcctga aagccttttt gcagcatctc ataggcggac tcgcggtcca
     8341 cctcatcttc atatttgcca tacaccgggg agtgattaat caggccatta cgctcatctt
     8401 ccgtcaccgg ccccattcgc gaacaaggtg cgatcaccat cgcccgttcc accactgaag
     8461 gacttccttt cgcatcgaga aacgagatta acgcctcgcc ggtccccagt tcctggattg
     8521 ccttttcggt atcaaacgcc ggattggccc gcatggtttg cgccgctgcc ttcactgctt
     8581 tctgatcttt aggcgtaaaa gcacgcaaag cgtgttgaac gcgattaccg agctgcccta
     8641 gcacgttatc cgggatatct gacgggtttt gcgaaacgaa ccagacgccc acgccttttg
     8701 agcgaataag ccttatcacc tgctcaatct tatccagcag tacctgtggc gcatcgttaa
     8761 acagcagatg tgcttcgtcg aagaaaaaca ccagttttgg tttctccaga tcgcccgctt
     8821 caggcaattg ttcatataac tctgaaagca tccacagcag gctggcggcg tacagtttcg
     8881 gcatctgata aagtttctcg gcgctgagga tattgataac gcctttaccg ttggcatcgg
     8941 tgcgcatcca gtctttgata tccagcatcg gctcaccaaa gaagtgttct gcgccttgct
     9001 gttccagcga cagtaatccg cgctggatgg caccaaccga tgcgctactg atatttccgt
     9061 actgattctg gaaggatttg gcgttatcgc cgatgtactg ggtaatcgcc cgcaaatctt
     9121 taaagtcgag cagcaacaat ccctgatcgt ccgcaatgcg gaagataata ttcagcacgc
     9181 cagattgcac atcgttgaga ttcaacagtc gtgccagcaa cagcggcccc agatccgaaa
     9241 ccgtcgcccg caccggatgg cctttctcgc caaagatatc ccacaccacc accggattgg
     9301 catgcggttg ccagtcgttg acgccgatat ttttcagtct tgcgagcagt ttttccgacg
     9361 ccgttcctgc ctgcgcgatg ccggtcagat cgcctttcac atcggccata aacaccggaa
     9421 caccgatttc cgacaatgac tctgccagtt tttgcaacgt gacagtttta cccgtccccg
     9481 ttgcgccagt aatcagcccg tgacggttag ccattcccgg cagtaaaaac agttctgtgt
     9541 ccggcgtgcg ggcaattaac aggggttcgc tcatgagatt tcctccagtt tcctgcctgg
     9601 agtataggca acgtccggca aagagaatat gtgtaatgaa gagaaaataa cctaaagcgt
     9661 gttaccgtga taaatctgat acccgaggtc aacagtgttg tggttgtgat cgttattctt
     9721 aatcttgctc agcaacattt gggcggccat ccgaccaata tcaaaacggg gagtaattac
     9781 gctggcgagg cttgggatca tttgcctgcc aatctccagc ccatgaaaac ccgcaatgga
     9841 aatttgttct ggcacggcga ggtttcgctc gcggcacagc agaagcgcac ccatcgcaat
     9901 atcgtcattg gtgcaaaata caccgtctaa atccgggttt gcgctaaggg catcacgcat
     9961 taactgcatt cctaaatgaa tggatgagat ggcgcgtgga ttcatgcgta atggggaaag
    10021 attatgcagc atcatcgcat cgcaataccc ctgataacgc tgttcatcgc gggtgtcatc
    10081 tttggaaccg agatacagaa ttttgcgcct gactcgctta tccagcatgg tacacaccat
    10141 gtcaaaagca gcctgccgat tatcaaaacc gacctccata tccagccgtt ctccctgtac
    10201 atccatcaat tccacgaccg gaatggtggc tgaacgcaga aatttcaccg tcctgatggt
    10261 gtgatatttt tccgaaagaa taatcccgtc aatgttataa gagagcaggt taatcaccga
    10321 ctcttcttcg gaatcgcgat cgtaattgta attagcaata agggtctgat aattatgcac
    10381 agaggtgacg gattctattc ccgcgagaat gtcagcgaag agttgatttt gaaatgacgg
    10441 aatcaagata ccgagggtat aactttgtgc attcaacaac atgccgggag cgcgattagg
    10501 gatataattg atctcttcca taattttggc gatgcgctcg cctgtttcct ttgccacctt
    10561 tttcggcgag cggatataac gactcacggt catttttgtt acgccagcca gcgtagcgat
    10621 atcctgtaaa gaaattctgt gattgcgcat aacttctccc aacgtcgggg gaatcttcaa
    10681 tcttcccccg aaactacatc acaatatttt attctttcag tgcaatacgg cgttaatggc
    10741 taacacgccc agcaaaccca taatagaaat aagggtttcc atcactgtcc aggtacgcaa
    10801 cgtttcacca accgtcagat taaaataccc tttaaatagc cagaagccag ggtcgtttac
    10861 gtgtgacgcg atcacgctgc ccgcaccagt tgccagtacc attaaagcgg gatcggcatg
    10921 ggtaacgttg ataatcggca acaccacacc cgcggtggta atggccgcga cggtggcaga
    10981 gcccagagcg atacgcaaca gcgccgcgac agtccagcac atcaataacg gcgaaagcgt
    11041 agttccggtc attaagtgtg aaatatactg cccgacacca ctatctacta atacctgctt
    11101 aaacgcgccg ccgccagcga taataaacac aatcatcgcg atagcgccta tagaatcccc
    11161 aatgatatcc atgatttgct cgatggtgcg tccattacgt cggcccagtg tgaaaatcgc
    11221 aataacaatg gcaataaaca gcgcaacggc agggttaccg acaaattcaa agaagaggcg
    11281 cacggtgtta gttttcggta acgtaatttc acaaacggcg gcgatagcca tcaggatgac
    11341 cgggatcact gcagcaaaaa tactgttcca gaaggagggc atctcctctt cgctaaacag
    11401 atgaggatta aataagcctt ccggtggtgc tttctcaaag cgagttagca gtttagaaaa
    11461 cagcggtcct gcgacaataa ctgtcggaat ggtaatgata aatccataca gtaaagtcgt
    11521 tccgagatta gcctcaaaga tagtcgcgat ggcagtaggc cctggatgtg gcggcagaaa
    11581 acagtgggtt acagagagcg ctgctaccat cggtacgcca acatacaaca ggggtaatcc
    11641 agatgatgcc acgatggtaa ataccaacgg caacagcagg acaaaaccca cttcaaaaaa
    11701 catggcgagg cccacaacca gaccggtgat cactagcgcc cattgcacgc gttttttacc
    11761 aaaagtagca atcagcgtag tggcgatacg ttgtgccgcg cccgtatcag aaatcagctt
    11821 gcctaacatg gccccgaaac cgaggatcat tgccagcccg ccgagcgtgc tgccgatacc
    11881 attttgtata gagtgcagga cggcctgtgc atccatccct tcggcaaatc cgacgacggc
    11941 agctaccaga acgagggcaa taaagccgtt aactttaaag ccgatcatca ggataagaag
    12001 cagcgcgacg cctgccgcaa taatgattaa tggcatagtc tttatctctt attagtgcca
    12061 gaaaagcagc gctttcgcgc tgcacgtagg gtggtgggta gggtggtggc tgttaaacag
    12121 ccactaacat gccgccatca acaaacaaca ggtggccgtt tacgaaatca gaggcttttg
    12181 aagaaaggaa caccgcagca ccaatcagtt cctgcggatc tccccagcgt gcggcggggg
    12241 tccgtttgca caaccaggcg gtgaaggctt cgtcctcaac cagtgcttta gtcatttctg
    12301 ttttgaaata gcccggcgca ataccgttga cctgaatatt gtggcgcgcc agctcgacgc
    12361 acatgccgcg ggtgagcatt tttaccgccc ctttcgatgc ggcataaggg gtgatggtgt
    12421 cacgtcccag ttcgctttgc atcgagcaaa tattaataac tttacctgcc ttgcgttcaa
    12481 ccatgtgacg agttaccgct tgcgatacca ggaacacggc ggtctggttt actgcgatca
    12541 catcattcca ctcttgttca gggaactcag taaaaggatg acggcgctgg ataccggcgt
    12601 tattcaccag cacatcaatg gggccgatgt ccttttcgat atgttcaacg gcggcatcaa
    12661 tttcatgttt atgagtaaca ttaaaaggtg cggcaacggc ctgaataccc tcctggtgga
    12721 gtttttctac agcaagttct gcgcgttcgg cagtaatatc attaataatt atttgtgcgc
    12781 catatttacc caggccggtt gccagtaaat agccaatgcc ctgtgctgaa ccggtaatca
    12841 agatattttt tcctgccagt gaaaatagat cgttcattgt attattcctt tatttatctc
    12901 ttagaaaaca agctggactt ttgctgcctg ggttttatca ccggcgaaac gtagcgcctc
    12961 ttccaggtca gtgaagagat attcagcact cagtaaaggc agtggattga taacgccatt
    13021 cgccagccat gacactgcgg tattaaattc gctggtaaaa cggaaagagc ctttgagtga
    13081 aatctcctta ccaatcaacg tcatcattgg gaattctgcc atcgcgcctc ccatccctac
    13141 ctgcaccatt acgccgcgtg cacgagtgac ctccagacag gtattcactg atgaaggatg
    13201 accggacact tcaaagctga catcgaaatg gcctttttcc gctttccagt gatccatgtc
    13261 gtcgttttgt gggtttacga gcacatccgc acccatctct ttggccagcg aaagggaacg
    13321 gggactcaca tcggcacaga caatttccgc ggaccccagt gttttcacag cactgacaat
    13381 caggcagcca atgggcccaa caccggaaac aaatactcgc ttgccctgta actcgtcggc
    13441 ctgatgtgcg gcatgaatcg cgacggctaa aggttcggca aaagccataa ccttttcgtc
    13501 agctttggcc ggataaggga cacattgcga cgtttcgacc attttataac gggtaaaacc
    13561 accatcaaca tgagggaaat acatggcact gccaaaaaaa cgcatatctg tacactgatt
    13621 ctcgttatgt tcaatgcagt atttgcagtg accgcacggt ttagacggat taatggctac
    13681 cgtttgccct tcatgtaatt ctgatgagtc gctatgaata actttaccga taacttcatg
    13741 acctaacacc atcggtgcct ttatcatgaa attacctact tttccttcct gataataatg
    13801 taaatcggaa ccgcaaattc cacctcgggt tatttgtact aatgttccat tattattcca
    13861 atctatcgtc tgctcggtaa cggcaacagt tttcttgtcc gcaacaacgc aggactgtgt
    13921 tttcacttgc ataaattcct cactggtcag gtagacacct cggaagcatt taagcggttt
    13981 taattgtcat tatttgtgat gaagatcacg tcagaaaatt gttacattac tatgttacgc
    14041 ataacgtgat gtgccttgta attcttatca gcagaaataa aaaaacgtga aattattatg
    14101 ccaccaggcg tagtatcgca gcaggtaaga tgattcagga gattttaaat ggcgggtgaa
    14161 agctttattt tgatgggcgt ttcagggagt ggtaaaacat taattggtag caaggttgcc
    14221 gcgttattat ctgctaaatt tattgatggt gacgatcttc atccagccaa aaatatagat
    14281 aaaatgtcgc agggtattcc attatctgat gaagatcgac ttccctggct ggaacgctta
    14341 aatgatgctt catacagtct ttataaaaag aatgaaacag gatttattgt ctgttcatca
    14401 ttaaaaaaac agtatcgtga tattttacgc aagggtagcc cccatgttca tttcctctgg
    14461 ttagatggcg actatgaaac tattctcgcg cgaatgcagc ggcgggctgg gcattttatg
    14521 ccggtagcgt tactaaaaag tcagtttgag gcgctggagc gtccacaagc agatgaacag
    14581 gatattgtac gcattgatat caaccatgat attgccaatg tcaccgaaca atgtcggcag
    14641 gccgtgctgg cgatacgaca agacagaata tgtgcgaaag ggggtagcgc ctcagatcag
    14701 cgctgcgaat gattttcaaa aatcggcttt cagaaccacg cggtaacgcg ccttaccgtt
    14761 gcgcacatgc tggatggcgt cgtttatttt cgacatcggg aacagttcgg tggtcggcgc
    14821 aaccttgctg cgggccgcaa agcgcatcag cttacgaagc tcataaggtg tgccggttgc
    14881 tgaaccagag acgctgcggt cgcccgcaat taacgtaaag gccggaacag acagcggcgt
    14941 gaggaccgca ccgacggtgt ggaaattacc gccatacgtc agcgcctcaa aataaggctg
    15001 ccagtcgagg ctgacgttga cggtgttgat aatgagatcg aactgccccg caagtgtttt
    15061 cagtgcctgc ggatcgcggc tattcaccac tttatcggca cccatcgcca gtacttcctg
    15121 ctctttcgca ggattcgaac taaaggctgt cacctcgcat cccattgcgt gcagaagttt
    15181 tatagcgata tgccccagcc cgccaatacc aattacccca acgcggctgg tagcagtgat
    15241 atggtgcatc aacagtggtt taaagaccgt gataccacca cacagcagcg gcccggcgga
    15301 ctcgatatcg atattttccg gcagtgggat cacccattgc cagtctgcac gcaacttctc
    15361 ggcaaaaccg ccgcgattca taattgttgg caccgcacct tgctcgcagt tgatctgatt
    15421 accgctaata caggcgtcgc aatgcccgca gctacgcgcc gtccagccaa tcccgacacg
    15481 ctgaccgacc tgcaaacctt tatcctgcgc ggcgctcccg agcgccacca cgcgcccaat
    15541 cacctcatgc ccggcaacca gcggatactg tgaaaatccc cattcgttgt cgatcatcga
    15601 cagatcggaa tggcagatcc cgcagtaatc cacctgcact tcaacatctt gtggcttcaa
    15661 ttcaccgggc tcgtactcat aaacttccag ttcgccgccc gcttcttttg cggcatagct
    15721 ttttatcatc gacatttttt ggtccttctc tggtgttgtc tgggaagtgt agagcatggc
    15781 agggcggggt gcctggagtg tgacaaaggt tacacatcgc tgtatgcaat gctgaaaatt
    15841 tcagcactta gcgaggtgcg agcaagctgg cgcttgcatg gtggcgtgcg acaggtataa
    15901 tccacaacgt tttccgcata cctcttcagt gccgaagtgg cgaaatcggt agacgcagtt
    15961 gattcaaaat caaccgtaga aatacgtgcc ggttcgagtc cggccttcgg caccaaaagt
    16021 atgtcaatag acctcaactg aggtcttttt tatgcctgaa attcagtatt tatctacctt
    16081 tcccgctata ttaactctct caaggtcaac cgatatcaac gtacttctac caacatatgt
    16141 tggtacagat gatggtattt ccggttcgat aatgcttgta ccaacaggga gggaatacgc
    16201 atggcattaa cagacatcaa agtcagaaca gctaagccaa cagataagca atataagttg
    16261 actgatggtg gcggtatgca tctgcttgtc catccaaatg gttctaagta ctggcgtttg
    16321 cagtaccgtt atgagggaaa gcaaaagatg ctggcacttg gggtttatcc tgaaatcaca
    16381 ctcgcggatg ccagaatacg tcgtgacgag ccacgtaagc tgcttgcgaa tggcgtcgat
    16441 ccgggagaca aaaagaaaag tgataaggtt gaacagagta aagcacgaac ctttaaagaa
    16501 gtcgcgattg agtggcatgg caccaataaa aagtggtctg aagatcatgc ccatcgtgtg
    16561 ctaaaaagtc tggaagataa tctttttgca gcgcttggtg aacgtaatat cgctgagtta
    16621 aaaactcgag atttattagc acctattaag gccgtagaaa tgtctggacg tcttgaagtg
    16681 gctgctcgtc ttcaacagcg c
//
LOCUS       DAZRUF010000076        16674 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZRUF010000076 DAZRUF010000000
VERSION     DAZRUF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.076"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            74..1213
                     /gene="arnB"
                     /locus_tag="ACHXOL_001988"
     CDS             74..1213
                     /gene="arnB"
                     /locus_tag="ACHXOL_001988"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR6808973.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1217..2185
                     /gene="arnC"
                     /locus_tag="ACHXOL_001989"
     CDS             1217..2185
                     /gene="arnC"
                     /locus_tag="ACHXOL_001989"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR6808974.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2185..4167
                     /gene="arnA"
                     /locus_tag="ACHXOL_001990"
     CDS             2185..4167
                     /gene="arnA"
                     /locus_tag="ACHXOL_001990"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR6808975.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4164..5054
                     /gene="arnD"
                     /locus_tag="ACHXOL_001991"
     CDS             4164..5054
                     /gene="arnD"
                     /locus_tag="ACHXOL_001991"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR6808976.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5054..6706
                     /gene="arnT"
                     /locus_tag="ACHXOL_001992"
     CDS             5054..6706
                     /gene="arnT"
                     /locus_tag="ACHXOL_001992"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR6808977.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6703..7038
                     /gene="arnE"
                     /locus_tag="ACHXOL_001993"
     CDS             6703..7038
                     /gene="arnE"
                     /locus_tag="ACHXOL_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR6808978.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7038..7424
                     /gene="arnF"
                     /locus_tag="ACHXOL_001994"
     CDS             7038..7424
                     /gene="arnF"
                     /locus_tag="ACHXOL_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR6808979.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7418..7684)
                     /gene="pmrD"
                     /locus_tag="ACHXOL_001995"
     CDS             complement(7418..7684)
                     /gene="pmrD"
                     /locus_tag="ACHXOL_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR6808980.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7794..9149)
                     /gene="menE"
                     /locus_tag="ACHXOL_001996"
     CDS             complement(7794..9149)
                     /gene="menE"
                     /locus_tag="ACHXOL_001996"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR6808981.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(9146..10108)
                     /gene="menC"
                     /locus_tag="ACHXOL_001997"
     CDS             complement(9146..10108)
                     /gene="menC"
                     /locus_tag="ACHXOL_001997"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR6808982.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(10108..10965)
                     /gene="menB"
                     /locus_tag="ACHXOL_001998"
     CDS             complement(10108..10965)
                     /gene="menB"
                     /locus_tag="ACHXOL_001998"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR6808983.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10980..11738)
                     /gene="menH"
                     /locus_tag="ACHXOL_001999"
     CDS             complement(10980..11738)
                     /gene="menH"
                     /locus_tag="ACHXOL_001999"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR6808984.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQHFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11735..13405)
                     /gene="menD"
                     /locus_tag="ACHXOL_002000"
     CDS             complement(11735..13405)
                     /gene="menD"
                     /locus_tag="ACHXOL_002000"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR6808985.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13494..14789)
                     /gene="menF"
                     /locus_tag="ACHXOL_002001"
     CDS             complement(13494..14789)
                     /gene="menF"
                     /locus_tag="ACHXOL_002001"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR6808986.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14868..15173)
                     /gene="elaB"
                     /locus_tag="ACHXOL_002002"
     CDS             complement(14868..15173)
                     /gene="elaB"
                     /locus_tag="ACHXOL_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR6808987.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15228..15689)
                     /locus_tag="ACHXOL_002003"
     CDS             complement(15228..15689)
                     /locus_tag="ACHXOL_002003"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR6808988.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15754..16671
                     /gene="rbn"
                     /locus_tag="ACHXOL_002004"
     CDS             15754..16671
                     /gene="rbn"
                     /locus_tag="ACHXOL_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR6808989.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         3847 a         4540 c         4285 g         4002 t
ORIGIN      
        1 aatggcaaaa tatacacaac actctttata gcaaatataa gtggacaggt attcaatggc
       61 ggaaggaaaa gcaatgtcag aatttttgcc tttttcgcga ccagcaatgg gcgtggagga
      121 actcgctgca gttaaagagg ttctcgaatc cggttggatc acaaccggtc cgaagaatca
      181 ggcgcttgag caagcctttt gccagttgac gggaaatcag catgccatcg cggtcagttc
      241 agccaccgcc ggaatgcata tcacgctaat ggcgttggaa attggcaagg gcgatgaagt
      301 gattacgcct tccctgacct gggtttcaac cctcaatatg atttccttgt tgggtgcaac
      361 gccggtaatg gtggatgtcg accgcgatac gctgatggtc acgcctgaag cgatcgagtc
      421 agccattacg ccacgcacta aagccatcat tccggtgcat tatgccggtg cgccagcaga
      481 tattgacgcc attcgcgcca ttggcgaacg ttacggcatc gcagttatcg aagatgctgc
      541 ccatgccgtc ggtacgtatt acaaagggcg acatattggc gcaaaaggta ccgctatttt
      601 ttcatttcat gccattaaaa atattacctg tgcggaaggt ggcctgattg taactgataa
      661 tgaaaacctt gcccgccagc tacggatgct gaaatttcac ggtctgggtg tcgatgccta
      721 tgacagacaa acctggggcc gtgcaccgca ggctgaagtc ttaacaccgg gctataagta
      781 caatctgacc gatattaacg ccgcgattgc cctgacacag ttagcaaaat tagagcacct
      841 caacacccgt cggcgcgaaa ttgcccagca atatcagcaa gcactggcag ctctcccctt
      901 tcagccatta agccttcccg cctggccgca cgttcacgcc tggcatctgt ttattattcg
      961 tgtcgatgaa caacgttgtg gtatcagtcg cgatgcgttg atggaagcgt taaaagaaag
     1021 aggcattggt accgggttac atttccgcgc cgctcacaca caaaaatatt atcgcgagcg
     1081 ttttcccacg ctgtcgttac cgaataccga atggaatagc gaacgcatct gttctttgcc
     1141 gctgttcccg gatatgacta ccgccgatgc cgaccgtgtc atcacagccc ttcagcaact
     1201 cgcaggacaa taagccatgt ttgaaatcca ccctgttaag aaagtctcgg tggttattcc
     1261 cgtttataac gagcaggaaa gcttaccgga attaatcagg cgcaccacca cagcctgtga
     1321 atcgttgggg aaagagtatg agatcctgct gattgatgac ggcagtagcg ataattccgc
     1381 gcatatgctg gtcgaagcct cacaagcgga gaacagccat attgtgtcta ttttgcttaa
     1441 ccgcaattac gggcaacatt cagcgattat ggcgggattc agtcacgtta ctggcgactt
     1501 aattattacc cttgatgccg atctccagaa tccgccagaa gaaatccccc gcctggtggc
     1561 aaaagccgat gaaggttacg acgtggtagg gactgtacgc cagaaccgcc aggacagctg
     1621 gtttcgtaaa acagcttcga agatgattaa caggcttatt cagcgcacca ccggcaaagc
     1681 gatgggtgac tacggttgta tgctgcgcgc ctatcgccgt catattgtcg atgcgatgtt
     1741 gcactgccat gaacgtagca cctttatccc gattctggcg aatatcttcg cccgccgtgc
     1801 cattgaaatt ccagtacatc atgccgagcg tgagtttggt gaatccaaat acagttttat
     1861 gcgcctgatt aatttgatgt acgacctggt gacctgcctt accacaacgc cgctacgtat
     1921 gctgagtctg ctcggcagca ttattgcgat tggaggtttt agcattgcgg tgctgctggt
     1981 gattttacgc ctgaccttcg gaccacaatg ggcggcagaa ggcgtcttta tgctatttgc
     2041 cgtgctgttt acttttattg gcgctcagtt tatcggcatg ggattgctcg gtgaatatat
     2101 cggcaggatc tacaccgatg tccgcgcccg cccccgctat tttgttcagc aagttatccg
     2161 tccatccagc aaggaaaatg aataatgaaa accgtcgttt ttgcctacca cgatatggga
     2221 tgcctcggta ttgaagctct gctggctgcc ggttacgaaa ttagcgccat ttttacccat
     2281 accgataatc ccgctgaaaa agccttttat ggttcggtgg ctcgtctggc ggcggaaaga
     2341 ggcattccag tttatgcgcc ggataacgtt aatcatccgc tgtgggtgga acgcattgcc
     2401 caactctcac cagaggtgat tttctctttt tattatcgcc atcttatttg cgacgagatt
     2461 ttgcagctcg ctcccgcagg tgcatttaat ctgcacggtt cgctgttacc aaaatatcgt
     2521 ggtcgcgcgc cgctgaactg ggtgctggtg aacggtgaaa cggaaacggg cgttacattg
     2581 caccgaatgg taaaacgtgc cgatgctggg gccattgtgg cgcaactgcg cgttgccatt
     2641 gcgccagacg atatcgctat tacgctgcat cataaattgt gccatgccgc gcgccagcta
     2701 ctggaacaga cattacccgc cattaaacac ggtaatattc tggaaattgc ccagcgcgaa
     2761 aacgaagcca cctgttttgg tcacagaacg ccagatgaca gtttccttga atggcataaa
     2821 ccggcatccg tactgcacaa catggtacgt gccgttgccg atccgtggcc gggtgccttc
     2881 agctatgttg gcaatcagaa attcaccgtc tggtcgtcgc gtgttcatcc tcatgccagc
     2941 aaagcacagc cggggagcgt gatttctgtt gcgccactgc tgattgcctg tggcgatggc
     3001 gcgctggaaa tcgtcaccgg acaggcgggc gacggcatta ctatgcaggg ctcgcaatta
     3061 gcgcagatgc tgggcctggt gcaaggttca cgcttgaata gccagcctgc ctgcaccgcc
     3121 cgacgccgta cccgggtact catcctcggg gtgaatggct ttattggcaa ccatctgaca
     3181 gaacgcctgc tgcgcgaaga tcattatgaa gtttacggtc tggatattgg cagcgatgcg
     3241 ataagccgtt ttctgaatca tccgcatttt cactttgtcg aaggcgatat cagtattcat
     3301 tccgaatgga ttgagtatca cgtcaaaaaa tgtgatgtcg tcttgccgct ggtggcgata
     3361 gccacgccga ttgaatatac ccgcaacccg ctgcgcgtat ttgaactcga ttttgaagag
     3421 aatctgcgca ttatccgcta ctgcgttaag taccgtaagc gaatcatctt cccgtcaact
     3481 tcagaagttt atgggatgtg tagcgataaa tacttcgatg aggaccattc taatttaatc
     3541 gtcggcccgg tgaataaacc acgctggatt tattcggtgt caaaacaatt acttgatcgg
     3601 gtgatctggg cctatggcga aaaagagggt ttacagttca ccctcttccg cccgtttaac
     3661 tggatgggac cacgactgga taaccttaat gcagcgcgaa ttggcagctc ccgcgctatt
     3721 acgcaactca ttctcaatct ggtagaaggt tcaccaatta agctaattga tggcggaaaa
     3781 caaaaacgct gctttactga tatccgggac ggtatcgagg cgttgtaccg cattatcgaa
     3841 aatgcgggaa atcgctgcga cggcgaaatt atcaacattg gcaatcctga gaacgaagcg
     3901 agcattgagg aactgggcga gatgctgctg gcgagcttcg aaaaacatcc gctgcgccat
     3961 cacttcccac cgtttgcggg ctttcgtgtt gtcgaaagta gcagctacta cggcaaagga
     4021 tatcaggacg tagagcatcg taaaccgagc atccgcaatg cccgccgctg cctggactgg
     4081 gaaccgaaaa ttgatatgca ggaaaccatc gacgaaacgc tggatttctt cctgcgcacc
     4141 gttgatctta cggataaacc atcatgacca aagtcggctt acgcattgat gtcgatacct
     4201 ttcgtggcac ccgtgaaggc gtgccacgtc tgctggaaat cttgagtaag cataatattc
     4261 aggccagcat ttttttcagc gtcggcccgg acaatatggg ccgccatctc tggcgactgg
     4321 tgaagccaca gtttttgtgg aagatgctgc gctcaaacgc ggcatcgctt tatggctggg
     4381 atattttact ggcaggtacg gcctggccag gtaaagagat tggtcatgcc aatgccgata
     4441 tcattcgtga agcggctaaa catcacgaag tcggcctgca cgcctgggat caccatgcct
     4501 ggcaagcccg tagcggtaac tgggatcggc aaacaatgat cgacgatatt gcacgcggtc
     4561 ttcgcactct ggaagagatt atcggtcaac cggtaacctg ttctgccgct gcgggctggc
     4621 gtgccgacca gaaggtgatc gaagcaaaag aagcgttcca tttgcgctac aacagcgatt
     4681 gtcgtggggc catgccgttc cgtccactgc tcgaatcagg aaaccctggc actgcgcaaa
     4741 ttccggtgac cttacccacc tgggatgaag tgattggtcg ggatgtgaaa gcagaagatt
     4801 ttaacggttg gttactcaac cgcatcctgc gagataaagg cacgccggtt tataccattc
     4861 atgcagaagt tgaaggctgc gcttatcagc ataattttgt ggatctcctc aaacgcgcag
     4921 ctcaggaagg cgtgacattt tgccctttaa gcgaactgtt atcagagacg ttgccgctcg
     4981 gacaagttgt tcgcggaaat attgccggac gtgaaggctg gctgggttgc caacaaattg
     5041 cgggtagtcg ctgatgaaat cggtacgtta ccttatcggc ctcttcgcgt ttattgcctg
     5101 ctattacctg ttaccgatca gcacgcgtct gctctggcaa cccgatgaaa cgcgttatgc
     5161 ggaaatcagt cgagaaatgc tggcatccgg cgactggatt gtgccccatc tgttagggct
     5221 acgttatttc gaaaaaccca ttgccggata ctggattaac agcattgggc aatggctatt
     5281 tggcgcgaat aactttggtg tgcgggcagg cgttatcttt gcgaccctgt taactgccgc
     5341 gctggtgacc tggtttactc tgcgcttatg gcgcgataaa cgtctggctc tactcgccac
     5401 agtaatttat ctctcattgt ttattgtcta tgccatcggc acttatgccg tgctcgatcc
     5461 gtttattgca ttctggctgg tggcgggaat gtgcagcttc tggctggcaa tgcaggcaca
     5521 gacgtggaaa ggcaaaagcg caggattttt actgctggga atcacctgcg gcatgggggt
     5581 gatgaccaaa ggttttctcg cccttgccgt gccggtatta agcgtgctgc catgggtagc
     5641 gacgcaaaaa cgctggaaag atctctttat ttacggctgg ctggcggtta tcagttgcgt
     5701 actgacggtt ctcccctggg gactggcgat agcgcagcgg gagcctgact tctggcacta
     5761 ttttttctgg gttgagcata ttcaacgctt tgcactggat gatgcccaac atagagctcc
     5821 gttctggtac tacgtgccgg tcatcattgc cggtagcctg ccgtggctgg gattactccc
     5881 cggtgcactg tacacaggct ggaaaaaccg caagcattcc gcaaccgtct atttgttgag
     5941 ctggacgata atgccgctgc tgtttttctc cgtcgctaaa ggtaaattgc ccacctatat
     6001 tctttcctgc tttgcatctc tggcaatgct gatggcgcat tacgctttgc tggcagcaaa
     6061 aaataatcct ctggcgctgc ggattaatgg ctggattaac atcgcttttg gcttcactgg
     6121 cattattgcc acatttgtgg tctccccgtg gggaccaatg aacacgccgg tgtggcaaac
     6181 cttcgagagc tataaagtct tttgtgcctg gtcgattttt tcgctatggg catttttcgg
     6241 ctggtacacc ttaacaaacg tcgaaaagac ctggtctttt gccgcgcttt gcccgctggg
     6301 gctggcgttg ctggtaggat tttcaattcc tgacagagtc atggaaggaa aacatccgca
     6361 attttttgtc gagatgacac aagaatcact tcagccaagc cgctatattc ttactgatag
     6421 cgtcggtgtt gccgcaggtc tggcatggag cctgcaacgc gatgacatca tcatgtatcg
     6481 ccagacaggt gagttgaaat acggccttaa ttatccggat gcgaaaggga gatttgtcag
     6541 cggtgatgag ttcgcaaact ggcttaatca acatcgtcag gaggggatta ttacactcgt
     6601 gctttcggtt gaccgcgatg aagatatcaa cagtctcgcc attccgcccg cagatgccat
     6661 cgatcgtcag gagcgtctgg tgctgattca gtatcgtccc aaatgatctg gctaacatta
     6721 gtctttgcca gcttgcttag cgttgccggg cagttgtgtc agaaacaggc aacctgcttt
     6781 gtggcgataa acaaacggcg caaacatatc gtgctgtggc tgggactggc gctggcttgt
     6841 cttggtcttg ccatggtgct ctggctgctg gtcttgcaga acgtaccggt aggcattgcc
     6901 tacccgatgt taagtctgaa ttttgtctgg gtgacgctgg ctgcagtaaa actgtggcac
     6961 gaaccggtat cgccgcgtca ctggtgtggg gtggcgttca ttattggcgg cattgtgatc
     7021 ctcgggagta cggtgtaatg ggcctgatgt ggggattatt cagcgtcatt attgcctcag
     7081 ttgcgcaatt aagcctgggt tttgcggcga gtcatctgcc gccgatgacg cacttatggg
     7141 attttatcgc ggctctgtta gcttttggcc tggatgcccg gatactgtta ctcgggctgc
     7201 tgggttatct gctttccgtt ttttgttggt ataaaacgct acataaactt gcgctaagca
     7261 aagcctatgc cttgctcagt atgagttatg tgctggtatg gattgcttca atggtacttc
     7321 ccggctggga agggactttt tcgctgaaag cactactggg agtagcctgt attatgagcg
     7381 ggttgatgct gattttcctg cccacgacaa aacaacgtta ctgagttttc cctgccactt
     7441 tacactgccg ttcccactca tcggggctat aactgcttgc cgagaggact ttcaccgtat
     7501 gcagcttttc acgattaata caataaagcg cattctgcaa aggcgagagt aaatccccca
     7561 ctttcacggc gaagtcactc ttcacttcgg cgatcatttt tatcgcgccg ccagcatcgc
     7621 agagcataag tacatgtctg ttatcttgtt tattgcagca cgattttttg accagccatt
     7681 ccattgcatt atcctgtttg ctaagagttt tcacatcaat ttacagatta acagataaac
     7741 ctattatcaa cggcaatgga atggcaaaat tgcgaagtga aactaatgat ttcttaatcc
     7801 tggcgttgca cccactcttt taacgcctga cgtgaaattt taataccgcc gtttttcagc
     7861 tccggcggta aagttagcca gcgcaccggt tgttgaaaac gagccagctt atctttcacc
     7921 cattcgccaa gatcaacggt ttgctggtca tactccacca ccgctaccgg tcgatgacca
     7981 aactccttgt cggcaacggg gacgataaac acctgcaaaa ccgcaggatg tgcagcaatt
     8041 acgcgctcga cttcttccgg ctgaataccc tctccgccac tgaagaacag attgtctaaa
     8101 cgtccgacaa tggtcagctt gccattatgc atctcaccgc gatcgcgcgt agcgtaccag
     8161 ccttcgtcat taaccagtga aaccagttgc ccgttacgcc agtaaccttc tgccatactg
     8221 gcagcccgca gccacacttc attattaacg attttcactt cccgacccgg cagcggcgaa
     8281 ccaacgtctg ccaggccgtc ggcttctttc gcacacaccg tggaggcaaa ctcggtcaga
     8341 ccatagccgc aaaagcaacg aatcccctgc tcgcgcgcct gttccgtcaa ctcgaccggg
     8401 atagccgcgc cgccaagtaa caccgctttc agggaaacgg aactgcggtt aaccagcaaa
     8461 cgccagagtt gtgttggcac cagtgaagcg tgagtacagc ctgccagcat ttgctccaat
     8521 ggctgtttat cacgcaccgt catccgcgca ccagcgtata accagcgcca cataattccc
     8581 tgaccggaga cgtgaaacag cggtaaagag agcaaccagt catcgtgatc gccaaacgga
     8641 atcagcgata acacaccttg cgcactggca agatgggcct gataagtatg gacagcggct
     8701 ttcggcaaac cggtagaacc cgaggtcaac gtcattgagc acagacgcgt cggctgccac
     8761 gtagcggcat gtgcgccttc aacccgctga atgtgcagcg acgttaatgc cggaaacgtg
     8821 ttttccccat ccggcaccag cgcaaattgc agcgtcagat tgggcagcaa ttcttcaagc
     8881 aacggttgcg gcagctgagg gttcacgggc aacacccgcg ccccgcattg cagtaacgcc
     8941 agccaggcga gcagcgtttg cggcgtattc cacgcccgca acatcacgcc gctgccctca
     9001 accacaccct gcgccgcaaa gcccgtagct aactcatcga cgcgagcaca aagctcgcgc
     9061 cagttgagtt gctcgtcatt aagacgtaag gcgatggctt ctccccgcac ttgccgccag
     9121 tgacgccacg gccagtcaga gaagatcata acaactgctc cagtacatca gcgtccacta
     9181 acggcagtgg gctccccggc cagcgacgaa cctgctgcgc ctgcatcaga tccagcgtgt
     9241 ccagtcccgg aatggtgtct ggcgttaacc aggcagcaat ccgcgccagt tgcgttagac
     9301 ctaagctcga ctcaatggaa gaactgatca ccgccgtcag ccccagcgca tgcgccgcct
     9361 gtacctgctc gcgtactttt tccagactgc ccgtgagcgt gggtttgata actaccgcgc
     9421 gcacgccctc ttcagccaca aaggcaaaat ccggctcgcg caggctttca tcccaggcaa
     9481 tggcaatgcc ggtttcacgg gcaaacgctc gcgaatcatc gcgggttttg cacggctctt
     9541 cgagaaacgc gatgcggtgg cgataatccg ggttaacata tttggcaaac tgctgacctt
     9601 tcagcggtgt ccaggcgcga tttgcgtcaa gacgcaaatg cagatccgga attgcctcca
     9661 gcaacagatt aaccaccatg ccgtcgcgca cggcttcgta caatcccact ttgaccttcg
     9721 ccactttctc gcctggcata tcagcaagtt tgaggatcag atcgtcagga tcgccattac
     9781 acagtggtgc cgcacggtaa ttggccgcct gcgggagcgt atctgccagc tccgccaatg
     9841 cacagcttac gccaaaggca acggaaggca tctgcggtag ctcgccatcg cctgccagcc
     9901 agttatttac ccaagcaagc agcacaattt gcgcctcttc ccaggtttcc tgactgaagc
     9961 ccggcagtgg ggagatctcc ccccaccctt cgcgctcgcc ttcacgcagg caaacataca
    10021 gcccgtcgcg ggtttttaac cgcctgtcgc gcagaaccat ccccgcgtcc atggggatct
    10081 gccagcggta tacctgcgcg ctacgcatta cggattccgt ttaaatttgc tgaaatcagg
    10141 ctgacgtttc tggttgaaag cgttgcgacc ttcctgacct tcttccgtca tgtagaacag
    10201 catggtggcg ttgcccgcca gctcctgcag acccgcctga ccgtcacagt cggcgttcag
    10261 cgcagctttc aggcagcgca gcgccatcgg gctgttttgc agcatttcgc ggcaccaacg
    10321 gacggtttct ttttccagat ccgccagcgg taccacggtg ttgacaaggc ccatatccag
    10381 cgcctgtttt gcatcgtact gacggcacag gaaccagatt tcacgcgctt ttttctgtcc
    10441 gacgatgcga gccatgtagg aagcgcccca gccgccgtcg aaggaaccga ctttcgggcc
    10501 agtctgaccg aagatggcat tatctgccgc gatagtcagg tcgcacatca tgtgcagaac
    10561 gtgaccaccg ccgatggagt agccagccac catcgcgaca accggtttcg gacaggtacg
    10621 gatctgacgc tggaagtcca gcacgttcag gtgatgtacg ccggaatcat ctttatagcc
    10681 gccgtaatca ccacgcactt tctggtcacc accggagcag aacgctttat cgcctgcgcc
    10741 agtcagaatg atcacgccga cgttgtcgtc ataacgcgca tctgccagtg cctggatcat
    10801 ctcttttacc gtcagaggac ggaaggcatt gcgcacctgc ggacgattaa tggtgatttt
    10861 tgcgataccg tcggtggatt tttcataacg aatgtcctca aaaccttcgg agcagtcgtg
    10921 ccattcaacc ggtgcgtaaa gcattgcttc atcaggataa atcatattgt gtcctttagt
    10981 cagaaacgca agatctgcgc cagacttgcg ataacgccag cgggattttc ccgatgcgcg
    11041 ttatgtccgg cgcgaggaat gacatggcag tcggcagcca gttccgccgc caaggcgcgg
    11101 aatttgctgt cacgttcacc acataaataa taaaacgcaa atgtgcgggc gctaaggtta
    11161 gcacgtaaat caggctggac ggcgagagaa gtcgcctcca gcatggcggc aagggttgcg
    11221 ccattattgt tactgcgcag cgccaccagc tcccggcgtt gatcgtcatt gagcgaggca
    11281 aaaacaggct gttgatacca gtcggcaaat accgccgtta acggttctgt gcgaaaatgc
    11341 tgcgcccatt ggcgatcgga acgctgacgt tccgcgcgtt gttcagcatt ttgcaaccca
    11401 ggatgcccgc cttcgacaac aaccccacaa agccccggca tcccctggca agccgccatc
    11461 atcgccaccc gtcccccaag cgagtacccc accaaccaga agttaaggat gttgtaacta
    11521 accagggttt tacgcagtaa gtcggtgaca tcatcaaatc catcgacgct aatcgccgcc
    11581 gaaccaccgt gacctgggag ataaacatac aaccgtgagt agtcggcaaa cgcctcgccc
    11641 acttcttgcc attcgtggca atcgccggaa aaaccgtgca aaaacaccag ccagggtaaa
    11701 cctggtttgc cgtgttttgc ctgcgcgtgc aggatcataa atggcttacc tgcgccagaa
    11761 gttgctggag cgtttgcgca ccatcggtgt cgttaaccac catttcaatt accgtggtgg
    11821 ttggcgtgcg ccaggcatcg gcaagtgccg tttcaagttc ctgccagttt tgcggacgat
    11881 gatatttcag ctcgaacatc gcggcggcgt gctcaaaatg gacgttttgc ggcatcagat
    11941 agaaacgctc gcgctcgctt ttcggcgttg gcagcagcga gaaaatttgc ccgccgttgt
    12001 tgttcaccac aattaatatc agcggcgcgg aaacctgacg caataacgcc agcgcgttga
    12061 gatcgtaaag tgcggagaga tcgcccacaa tcgccagcgt cggtttgccg cttgcccgct
    12121 gaacgccggc ggcagtcgag agcagcccgt cgataccgct ggccccacgg ttgctgtaca
    12181 ccgggtaacc tgccggaagt tgcgaaagcg catcaatcag acgtaccacc aggctgttac
    12241 cgacaaacaa ttgcccctgt tcaggcagat agtcgctgat gcgatgcgcc agttgcgctt
    12301 cgccaaacgc atcgcgacgg gcaataaccg cctgcattgc ctgttccgcc aggcgcggga
    12361 tttcaacgca ccagggctgg cgtttttctg ccggatgcag ctccagccag tcggcaatat
    12421 tggcaattaa gcggcgtcca cgatggtgcg ccggatcaag ccgcccttcg atgtcatcca
    12481 caatccagta ctcttcgggt tcacagcttg cctgccattg caggagccgt ttgcccgtca
    12541 ggctgcttcc cagttgcacc acaatttgcg cctgttgcag ttcgctggtc gctttggcat
    12601 tgcctaacca gagatcggca cacggcagcg gctgtccggt ttgcgacagc acgtcgccaa
    12661 tcagcggcca gccaagagtt tgcgcccaca gtgcaacttt tttgccctct tccgcactca
    12721 tgcgcccggc aaccaccacg ccgcgttttt gtcgccagaa gaaccagtcg cgctgttttt
    12781 cactttccag acgaggcgct tcacgcagcc acggtttgtc gtcctgccac cagtcgccca
    12841 gccgctgttg ccagctaagc ccggtgtcgt ccatttcgcc atacagcggt tcggcaaacg
    12901 ggcagttgat atggactcca ccagaatgaa gcgtaccgag agcatggtcg atggtagaaa
    12961 ccagccaacg tgcggggata tcctgggtcg ggcgcggcaa tgaaatactg tgcgtggggt
    13021 gagaggcgaa cattcccggc tgacgaatcg cctgattcgc gccgcagtca atcagctccg
    13081 gcgggcgatc ggcggttaac aggatcagtt tttctccggt taacccagct tcaatcagtg
    13141 ccggatagag atttgctacc gccgtaccgg aggtcacaat caccgccacc ggctgcttgc
    13201 tgactttcgc cagccccagc gccagatgcc ccaacccacg ctcatcgaaa tgggtgtggt
    13261 gaatgaatgc ggaattctcc gccgccgcta acgttaacgg tgtagaacgc gagccagggg
    13321 cgatacagat gtgtctgacg ccgtgacgcg ttaatgcttc cagaatgacc gccgcccagc
    13381 gtcggttaaa tgcgcttact gacatgagtt tgtccggtat caatattgcg gctaagtata
    13441 aggagctaca aaaatagatt attgatatga atcggtaatg atgcgactca ttactattcc
    13501 atctgtaata aagtacgcag ccctgccgct ttgttgtcga tttcctgcca ctcttgctcg
    13561 gggtcggaac cacggacaat gcccgcgcca gcatataatc gcacgacatt gccgctaatt
    13621 tttgctgagc gcagggaaac gcagaattcg ctttgctgta atgagagata gcccgcagaa
    13681 ccggcgtacc attctcgggt gaacggttcg tgacgggcga taaactgtcg cgccagatcg
    13741 cgcggtaagc cagcaactgc tgccgttggc tgcaactgat gtaaacagat cacattatcc
    13801 gctttgttga gtgaagtcca gatacagcgg cgaagatgct gcactttacg cagacgcagt
    13861 acctgcggcg gtaaaacatc cagcgtctgg gtatcggcct gtaaccgttg gcagatatct
    13921 tccaccacca gcatattctc gcgctggttt ttatcatccg ccatcagcca ctcacctaac
    13981 tgctgcgcct gcttatcatc aggatgattt gctaccgttc ccgccagcgc ttcagtacgc
    14041 agcgctttgt cacgccgccg ccataaccgt tccggtgaag agccaagaaa agcattttcg
    14101 ccatcaaagg ccatgtaaaa atggtagcaa ttcagattca gtcgacgact ggcagccatc
    14161 atcgccgccg cgttgaccgg acttgcgaaa tgcaggtcag ttgcccgagc gagcaccact
    14221 ttgtcgagct caccttcggc gatggttttc gttgccagtt cgattaattg cgtccagccc
    14281 gttttgtccg gccagtgttg ttctcgcgtg gtggttaaat gtaacccagg caagggcttg
    14341 atactcacca gtgtggcgat aaattctctt gccttaatcg catcgtgctg aagggagctt
    14401 tcgctgaata gcgtcagccg cagcgtggct ttaccgccac agcgtcgcca ttccaggcgt
    14461 ggtaaaagta aattgccctg cgacgggtca aacgcattca gcccccaaat gcgtaagtcg
    14521 gcgtgttccg ggtgctggcg aagaaaacgt tgtgcctggt ccaacgacgt aaaacgggta
    14581 atcgcgccca ggacggcagc ttcttcatca ccattacgtt gttgccagta gaattgcggg
    14641 tatgtttgct gactggccag ccagctcaag gcatcaaaag cgtctttgag agggaaagga
    14701 atatcgataa cccgaatgcc gggtgtcgcc ggaatctctt gcgacaaatg gcgcagtaga
    14761 ttttccagcg ccgtagtaag tgattgcacg cggacctctc cctgttaaaa gcctcacatt
    14821 atacggggta ctacaaaaaa aatgcagtac cccggtgtag ggaggtttta acggcgtgcc
    14881 agcaacagtc ctagtaccag cccaacggcc gcgcccacac caattccttg ccagggtttt
    14941 tcgtggacgt agtcatcagc acgataaact gcctgcttcg cccgatagta ataactgtct
    15001 gaagcctggc taacccgttt tttcacatca tccagcgctt tttctgcacg cgctttcagc
    15061 tcaacatatt tctgatcggc gggatcgcca gaggagcgga gcacctcttc cagtgtttca
    15121 ctaagcagcg tcaggtcgtc atcgatacgt gtatcaccaa actgattaga cattctcgtt
    15181 ctccatttgc gtaaaacctg tcactaacta tagacaaggg ttgttgatta cgcctgaatt
    15241 acctcgcgtg ccatcccaat gtgtgggata ccatcttctt catagacctc cgtcacaggg
    15301 ataaaaccga aactctggta gaagttttgc agatgcgcct gcgccccgag gtaaacaggc
    15361 ttatcaggcc agtgatgcgt gcaggtgtcg agggttttac tcatcagttg ctgccccact
    15421 ttttcgccac gcaacgcttc actgacaatc acccgaccaa taacgaccgg ctcaagatca
    15481 tcatcacttt tcagaatcct cgcatatgcc accagttcat catttttcca accgagaata
    15541 tggcgattat cccccgtcaa atcatcacca tcgatatcct gataagggca attctgctcg
    15601 acaacaaaca ccgcgcaacg cagttgtaat aaggcataaa gctgggaaac cgataattca
    15661 gagtgatgca gatcttgcca ttcaatcata atgctctcct tttagatgta tgccttatac
    15721 tagccggaat attttttgaa acgtgatgaa ctcatggaat taattttttt aggtacttca
    15781 gccggtgtgc caacccgcac gcgcaatgtc acggcaatat tgcttaacct gcaacatccg
    15841 acccagagcg gactttggtt gtttgactgc ggtgaaggca cccagcatca gctactgcat
    15901 accgccttta accctgggaa actggacaag atttttatca gtcaccttca tggcgatcat
    15961 ctctttggtt tacccggctt gctgtgcagt cgttctatgt caggcattat ccaaccctta
    16021 acgatttatg gtccgcaagg tatccgtgaa tttgtggaaa ccgcgctgcg gattagcggc
    16081 tcatggaccg attatccgct ggaaattgtc gaaattggcg ctggcgaaat tctcgatgat
    16141 ggcctgcgca aagtgaccgc ttatccgatg gaacacccac tggaatgtta tggctatcgt
    16201 attgaagaac atgataaacc gggtgcatta aatgcccagg cattaaaagc tgctggcgtg
    16261 ccgcctggcc cactgtttca ggaattaaaa gcgggcaaaa caatcacgct ggaagatgga
    16321 aggcagatta acggcgcaga ttacttagct gctccagtgc caggtaaagc gctcgctatt
    16381 ttcggcgata ccggcccctg cgatgccgca cttgacctgg ctaaaggtgt cgatgtcatg
    16441 gtgcacgaag cgacgctgga tataaccatg gaagccaaag ccaatagtcg cggccatagc
    16501 tctacacgcc aggctgcgac actagcccgt gaggctggag tcggcaagct aatcattacc
    16561 cacgtcagct cgcgctatga tgacaaaggt tgtcaacacc tgttacgtga atgcaggtca
    16621 attttcccgg cgactgaact ggcgaatgat ttcaccgtgt ttaacgttta actc
//
LOCUS       DAZRUF010000077        16662 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZRUF010000077 DAZRUF010000000
VERSION     DAZRUF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.077"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..262)
                     /locus_tag="ACHXOL_002005"
     CDS             complement(<1..262)
                     /locus_tag="ACHXOL_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR6808990.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
     gene            369..665
                     /locus_tag="ACHXOL_002006"
     CDS             369..665
                     /locus_tag="ACHXOL_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR6808991.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(799..1224)
                     /gene="arsC"
                     /locus_tag="ACHXOL_002007"
     CDS             complement(799..1224)
                     /gene="arsC"
                     /locus_tag="ACHXOL_002007"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR6808992.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="ACHXOL_002008"
     CDS             complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="ACHXOL_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR6808993.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="ACHXOL_002009"
     CDS             complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="ACHXOL_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR6808994.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3472..3645)
                     /locus_tag="ACHXOL_002010"
     CDS             complement(3472..3645)
                     /locus_tag="ACHXOL_002010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6808995.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3673..3756
                     /gene="dinQ"
                     /locus_tag="ACHXOL_002011"
     CDS             3673..3756
                     /gene="dinQ"
                     /locus_tag="ACHXOL_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR6808996.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="ACHXOL_002012"
     CDS             complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="ACHXOL_002012"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR6808997.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="ACHXOL_002013"
     CDS             complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="ACHXOL_002013"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR6808998.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6279..8321
                     /gene="prlC"
                     /locus_tag="ACHXOL_002014"
     CDS             6279..8321
                     /gene="prlC"
                     /locus_tag="ACHXOL_002014"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR6808999.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8329..9081
                     /gene="rsmJ"
                     /locus_tag="ACHXOL_002015"
     CDS             8329..9081
                     /gene="rsmJ"
                     /locus_tag="ACHXOL_002015"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR6809000.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="ACHXOL_002016"
     CDS             complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="ACHXOL_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR6809001.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10765..10818
                     /locus_tag="ACHXOL_002017"
     CDS             10765..10818
                     /locus_tag="ACHXOL_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809002.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="ACHXOL_002018"
     CDS             complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="ACHXOL_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR6809003.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11742..12077
                     /gene="uspB"
                     /locus_tag="ACHXOL_002019"
     CDS             11742..12077
                     /gene="uspB"
                     /locus_tag="ACHXOL_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR6809004.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="ACHXOL_002020"
     CDS             complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="ACHXOL_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR6809005.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14052..15254
                     /locus_tag="ACHXOL_002021"
     CDS             14052..15254
                     /locus_tag="ACHXOL_002021"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR6809006.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15570..16622)
                     /locus_tag="ACHXOL_002022"
     CDS             complement(15570..16622)
                     /locus_tag="ACHXOL_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFR6809007.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4259 a         4248 c         4344 g         3811 t
ORIGIN      
        1 acccggcttt cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg
       61 gggtaacggc tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca
      121 tctgctggat tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca
      181 ctataaacag caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca
      241 gcatcgcaag ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa
      301 tcgaaatgaa attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg
      361 ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc
      421 gtttgttcat atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt
      481 tgcaaaagca gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat
      541 cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg
      601 caatgctgga agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg
      661 cctgatgcgc tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg
      721 tcggataaga cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg
      781 gcagtggata tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc
      841 atcttccttg gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga
      901 agggcggcac aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg
      961 gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag
     1021 ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc
     1081 aatgagtttg accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg
     1141 ttctgtgccg ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt
     1201 gtgataaatg gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag
     1261 cgccagcgca gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa
     1321 gtaatatccc cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt
     1381 agccaggcta ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata
     1441 aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac
     1501 cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc
     1561 gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc
     1621 cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac
     1681 aatctgccag ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc
     1741 gacgacaaat aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg
     1801 ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt
     1861 agcaggatct ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg
     1921 aatatctttg cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc
     1981 caccggcacc atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac
     2041 gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc
     2101 cgccatcacg aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat
     2161 gacaatcggc gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc
     2221 accgagcagg acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac
     2281 gtgcagcgcc gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac
     2341 ggcgataaac gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg
     2401 atgaaccacg cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc
     2461 taaacctttc ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag
     2521 taacataatg cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt
     2581 taactgcaaa tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc
     2641 gcctgaacct tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct
     2701 ggaatatgcg gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc
     2761 ccgctttcac gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca
     2821 gtacaaagat cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga
     2881 cgggtttcat cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc
     2941 tcctgattgt ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact
     3001 taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt
     3061 tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc
     3121 agcctgagtt ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat
     3181 tagccagtgg ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc
     3241 ggtatatatc gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg
     3301 tccgaatccg gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag
     3361 ttggtggctc tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac
     3421 tgaaactatt gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt
     3481 tagctctgag gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga
     3541 agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa
     3601 tgagacatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca
     3661 tggagaaaac gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg
     3721 gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta
     3781 agagcacact actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc
     3841 gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc
     3901 accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc
     3961 ttctcttctg gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg
     4021 gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc
     4081 tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg
     4141 gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag
     4201 agacggcgac ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc
     4261 accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt
     4321 tcgttagttt taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca
     4381 atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg
     4441 ctaccatcgg catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct
     4501 tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc
     4561 gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc
     4621 tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg
     4681 aagaagccat cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga
     4741 ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt
     4801 ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg
     4861 ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc
     4921 cagttgaact tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct
     4981 tcacggattt gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag
     5041 gtgccgccca gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg
     5101 ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc
     5161 atgacagagt gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca
     5221 gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg
     5281 gaaccagttt gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg
     5341 gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta
     5401 ccgccagttc aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc
     5461 gcttaatttg ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac
     5521 gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg
     5581 gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct
     5641 ggaaaccgtc ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca
     5701 acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct
     5761 ggcgagcaat caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt
     5821 ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg
     5881 cgataccttc gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc
     5941 cggcccctgc gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga
     6001 tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt
     6061 ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg
     6121 cgcacaaaag cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg
     6181 tttctcattg aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt
     6241 tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc
     6301 cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc
     6361 aggcgctgaa cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca
     6421 cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatctttt
     6481 ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc
     6541 aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata
     6601 aagcgtaccg cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga
     6661 aagcggttga taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga
     6721 aacagcagcg ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca
     6781 acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg
     6841 cggggatgcc agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg
     6901 aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca
     6961 accaggctct gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc
     7021 cgaacgccgg taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg
     7081 aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg
     7141 cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc
     7201 aaggcgaaaa agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg
     7261 agttgcagcc gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca
     7321 tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa agcggttaac ggcctgtttg
     7381 aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc
     7441 atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct
     7501 acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag
     7561 gccagatgcg taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact
     7621 tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt
     7681 tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt
     7741 ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact
     7801 ggtgctggga gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc
     7861 tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta
     7921 ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg
     7981 atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc
     8041 catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg
     8101 cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct
     8161 ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga
     8221 gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc
     8281 agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc
     8341 ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg
     8401 ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg
     8461 cgcaagcgtg atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg
     8521 gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt
     8581 aaaggcgatt atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt
     8641 gtgctggctt cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg
     8701 ctgctcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag
     8761 gagcgtttgc agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc
     8821 ccgcaggtgg tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa
     8881 aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg
     8941 gagcctgcac gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca
     9001 ccgctagcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc
     9061 tatgcaggta cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg
     9121 caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa
     9181 cagcatcact actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata
     9241 gacgggtaac gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac
     9301 atagccgccc agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc
     9361 catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa
     9421 ttcacccagg ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg
     9481 tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa
     9541 catgccgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt
     9601 gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa
     9661 gctgaccggg tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa
     9721 gttcagcgac gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag
     9781 gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa
     9841 gaagatgatg gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt
     9901 gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag
     9961 tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg
    10021 acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac
    10081 tgaataaccg tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat
    10141 attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata
    10201 gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt
    10261 ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc
    10321 aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc
    10381 gacatagccg ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac
    10441 aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg
    10501 cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg
    10561 aggttgctgc agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc
    10621 atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga
    10681 ttcttaatat tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg
    10741 atcacactaa tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat
    10801 tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc
    10861 caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat
    10921 tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga
    10981 cgtgcggaag acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc
    11041 atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg
    11101 gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct
    11161 tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca
    11221 gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag
    11281 actgctttct ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt
    11341 ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc
    11401 ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa
    11461 agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct
    11521 tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct
    11581 ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca
    11641 caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc
    11701 cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat
    11761 tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac
    11821 gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag
    11881 ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct
    11941 atgcccaacg ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc
    12001 gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat
    12061 tgatgatttg gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa
    12121 ctgacccgct caagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc
    12181 ctacataatc tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca
    12241 cgccgcatcc ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc
    12301 gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa
    12361 agcagtaccg cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta
    12421 cgctgtaagc cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga
    12481 gtagtggaaa ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc
    12541 tgggcagaca tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc
    12601 gttgccacac ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg
    12661 atgatccaca ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac
    12721 aggcgctgat cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa
    12781 acgcacagca taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt
    12841 tccacatcgg tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga
    12901 tggcagtgga actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca
    12961 gcaccggtag cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg
    13021 atggcatcac gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct
    13081 gctggcgcga cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc
    13141 gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac
    13201 ggcggctttt tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg
    13261 cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc
    13321 aggccgacaa taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg
    13381 agtgcatcca ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc
    13441 gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc
    13501 cagataatcg ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata
    13561 ttaagcagca gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc
    13621 agcaaaacac ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc
    13681 gcgcgggtat agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct
    13741 tcgtagaaca gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg
    13801 ccagcaaaca aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt
    13861 atcagtgact ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg
    13921 tatggatttc actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt
    13981 aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag
    14041 aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt
    14101 tctgttctgc gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa
    14161 aaccagggcg caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg
    14221 tcgaaccagg cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt
    14281 ttacccagtg ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa
    14341 cgttagggca actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg
    14401 agtgcgagaa gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg
    14461 atgaaacagg cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca
    14521 tggcgaccgg tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg
    14581 ccgaacaatt tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc
    14641 ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta
    14701 ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg
    14761 gaccggcggt gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc
    14821 tgctaccgga tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc
    14881 agagcctgaa aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc
    14941 aactcgggca aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac
    15001 tgattagcac attgaccgac tggcgcgtac aacccaacgg cactgaaggc tatcgcactg
    15061 ccgaagtgac gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc
    15121 gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg
    15181 gctataactt ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag
    15241 cgaagtcgtc ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag
    15301 ctaattttga tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct
    15361 ccaggcttaa tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt
    15421 tataaaatat gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac
    15481 tatgcattaa tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt
    15541 caacgctggt tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt
    15601 gaaacgattg agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga
    15661 ataaaatatc ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa
    15721 aacaggcagg tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc
    15781 cagaccagtg ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa
    15841 ataagaataa tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc
    15901 ccctgcagta ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct
    15961 ttagttgaaa acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag
    16021 gcacagattg cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca
    16081 gcactacgta acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg
    16141 gcaatgaggc aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg
    16201 ctccccgctg catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat
    16261 acacaggcgg caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc
    16321 atcacatcat tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca
    16381 tagccaataa tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa
    16441 atgataataa atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca
    16501 tgacccgcga cgaaatactc gctttgccct gaactttcca gcacaaagcc acctaacgcg
    16561 atgcaaatta atcccataat caaagggatt aatttgaaaa gccacccgat atatatgttc
    16621 actttaaact cctcaggtat ccctgctaat aatgaaatgt gc
//
LOCUS       DAZRUF010000078        16430 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZRUF010000078 DAZRUF010000000
VERSION     DAZRUF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.078"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHXOL_002023"
     CDS             <1..266
                     /locus_tag="ACHXOL_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR6809008.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="ACHXOL_002024"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="ACHXOL_002024"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR6809009.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHXOL_002025"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHXOL_002025"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR6809010.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="ACHXOL_002026"
     CDS             complement(1570..3270)
                     /locus_tag="ACHXOL_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR6809011.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="ACHXOL_002027"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="ACHXOL_002027"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR6809012.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="ACHXOL_002028"
                     /pseudo
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="ACHXOL_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            4828..6600
                     /locus_tag="ACHXOL_002029"
     CDS             4828..6600
                     /locus_tag="ACHXOL_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR6809013.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="ACHXOL_002030"
     CDS             6593..8374
                     /locus_tag="ACHXOL_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR6809014.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="ACHXOL_002031"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="ACHXOL_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR6809015.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="ACHXOL_002032"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="ACHXOL_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR6809016.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHXOL_002033"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHXOL_002033"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR6809017.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="ACHXOL_002034"
     CDS             11336..11908
                     /locus_tag="ACHXOL_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR6809018.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="ACHXOL_002035"
     CDS             complement(11939..12250)
                     /locus_tag="ACHXOL_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR6809019.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="ACHXOL_002036"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHXOL_002036"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="ACHXOL_002037"
     CDS             12629..12982
                     /locus_tag="ACHXOL_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR6809020.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHXOL_002038"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHXOL_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="ACHXOL_002039"
     CDS             complement(14736..15206)
                     /locus_tag="ACHXOL_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR6809021.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHXOL_002040"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHXOL_002040"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR6809022.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /locus_tag="ACHXOL_002041"
     CDS             complement(16044..16262)
                     /locus_tag="ACHXOL_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR6809023.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..>16430)
                     /locus_tag="ACHXOL_002042"
     CDS             complement(16288..>16430)
                     /locus_tag="ACHXOL_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hha toxicity attenuator"
                     /protein_id="HFR6809024.1"
                     /translation="YLDDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASL
                     SC"
BASE COUNT         3893 a         4003 c         4437 g         4097 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgtaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt
//
LOCUS       DAZRUF010000079        16401 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZRUF010000079 DAZRUF010000000
VERSION     DAZRUF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.079"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            3..1409
                     /locus_tag="ACHXOL_002043"
     CDS             3..1409
                     /locus_tag="ACHXOL_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809025.1"
                     /translation="MIDNSTLTAVQRIEGKVKTRSKDSVDTDIIAFENYIQSILFYDH
                     VIAVDDYIPEYRGSRISSFREILFLDKAQYGLDEIESEAKKISDALKPKIRGGTFVND
                     DFKKLIELLQTHIICTWDISSSVYHLTLKNLSDGGKEFEKYGNIAAAIFSELRDAAES
                     GNRTNGDVELLDRFGNPIRKGYKVPGAKWGDGTSAGEASDAIKAFVASLVWLANRAVF
                     YSLTAKYLQADTFLYPLRQAYQQTYLSQNCQYGFDYAKHIVENFSTSLSHDLIDIHSG
                     GKAVATATSLPLFSAWLAKETGDPSCIVEAAYNIRDNNEFVRAREKMREIKRLFDENE
                     LPQANKSVRALLTEVSKESDYIRTKYGLKTRQGVPVTKLVHVYNTVAAINSLPKLPTF
                     NFKVKVPDFLYDLKKPKGFRALYRNLTNDLSTVWSLGEARDILGSKVVIDNHARIYSP
                     KHEEPRFKNSHSPYKSPM"
     gene            complement(1524..2051)
                     /locus_tag="ACHXOL_002044"
     CDS             complement(1524..2051)
                     /locus_tag="ACHXOL_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR6809026.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKDFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIRYVEKDVTAIRPDGLSVVEVPDITANRRADISGNWMFKDCTVI
                     KRIYTAEELQQQAENRKARLLADAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(2055..4373)
                     /locus_tag="ACHXOL_002045"
     CDS             complement(2055..4373)
                     /locus_tag="ACHXOL_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR6809027.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSDSE
                     TLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSAINSDSETLAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKGSWSYANNKIITDTGCGDIHLAGCVVEVMGTKSAITIRVTTPTTSSGGGTT
                     SAQFTYINHGDGYSPGWRRDWNRQGDSMTGTINQDGGSQNAYMSTALCSGTRGGKKYL
                     RKFRGGEGDTIWHETVQGGVIRWATGNNDAQEELSLSSAYGLRSRGEITSLSANGLRI
                     AYGNYGFFIRNDGGSTYLMLTASGDKFGTWNGLRPLTINNANGGVSMGHGLSVTGDIV
                     SSTKVRAGSGKKFTVSSSNTSTKEAAFNLWGNSSRPVVAELGDDAGWHFYSQRNTDNS
                     ITFAVNGQVSPSNYGNFDSRYVRDIRLGGAATYKPANNGMTWTHQAPSGCVYSGIIVQ
                     DTGSNSADNIGGVYYRPVQKYINGTWYNVAQV"
     gene            complement(4384..4914)
                     /locus_tag="ACHXOL_002046"
     CDS             complement(4384..4914)
                     /locus_tag="ACHXOL_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR6809028.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVRIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(4907..5815)
                     /locus_tag="ACHXOL_002047"
     CDS             complement(4907..5815)
                     /locus_tag="ACHXOL_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFR6809029.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTLPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(5820..6167)
                     /locus_tag="ACHXOL_002048"
     CDS             complement(5820..6167)
                     /locus_tag="ACHXOL_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR6809030.1"
                     /translation="MTARYLGMNRSDGLTVSDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEARVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(6164..6799)
                     /locus_tag="ACHXOL_002049"
     CDS             complement(6164..6799)
                     /locus_tag="ACHXOL_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR6809031.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(6866..7318)
                     /locus_tag="ACHXOL_002050"
     CDS             complement(6866..7318)
                     /locus_tag="ACHXOL_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR6809032.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIHYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(7311..7778)
                     /locus_tag="ACHXOL_002051"
     CDS             complement(7311..7778)
                     /locus_tag="ACHXOL_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6809033.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(7741..7986)
                     /gene="lysC"
                     /locus_tag="ACHXOL_002052"
     CDS             complement(7741..7986)
                     /gene="lysC"
                     /locus_tag="ACHXOL_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFR6809034.1"
                     /translation="MLCAGCTSAQPAQTPVIVHNACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(7886..8311)
                     /gene="lysB"
                     /locus_tag="ACHXOL_002053"
     CDS             complement(7886..8311)
                     /gene="lysB"
                     /locus_tag="ACHXOL_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFR6809035.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRALLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRTACADASDCPQRLPESEPLPDAGQ"
     gene            complement(8299..8724)
                     /locus_tag="ACHXOL_002054"
     CDS             complement(8299..8724)
                     /locus_tag="ACHXOL_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFR6809036.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VIAGGVGVYAAEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALMVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRLEGREPCQN"
     gene            complement(8739..9236)
                     /locus_tag="ACHXOL_002055"
     CDS             complement(8739..9236)
                     /locus_tag="ACHXOL_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFR6809037.1"
                     /translation="MPVISTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(9236..9517)
                     /locus_tag="ACHXOL_002056"
     CDS             complement(9236..9517)
                     /locus_tag="ACHXOL_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR6809038.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(9521..9724)
                     /locus_tag="ACHXOL_002057"
     CDS             complement(9521..9724)
                     /locus_tag="ACHXOL_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR6809039.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(9724..10233)
                     /locus_tag="ACHXOL_002058"
     CDS             complement(9724..10233)
                     /locus_tag="ACHXOL_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR6809040.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFSRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(10333..11076)
                     /locus_tag="ACHXOL_002059"
     CDS             complement(10333..11076)
                     /locus_tag="ACHXOL_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFR6809041.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALVHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(11080..12152)
                     /locus_tag="ACHXOL_002060"
                     /pseudo
     CDS             complement(11080..12152)
                     /locus_tag="ACHXOL_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
     gene            complement(12211..13065)
                     /locus_tag="ACHXOL_002061"
     CDS             complement(12211..13065)
                     /locus_tag="ACHXOL_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR6809042.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            13239..15011
                     /locus_tag="ACHXOL_002062"
     CDS             13239..15011
                     /locus_tag="ACHXOL_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFR6809043.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKVSVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            15011..16045
                     /locus_tag="ACHXOL_002063"
     CDS             15011..16045
                     /locus_tag="ACHXOL_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR6809044.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
BASE COUNT         3669 a         4586 c         4264 g         3882 t
ORIGIN      
        1 cactgattga taattcaact ctaactgcag tccaaagaat cgaggggaaa gtaaaaacaa
       61 gatcgaagga ctcagtagat acagacataa tagcatttga gaactatata cagtctattt
      121 tgttctatga tcacgttatc gcggttgatg attatattcc agagtaccgt gggagtagga
      181 tttcaagttt cagagagata ttatttcttg ataaagcaca atatgggttg gatgaaattg
      241 aaagtgaagc aaaaaaaata tctgatgcat tgaaacctaa aattcgtggt ggaacatttg
      301 ttaatgacga ttttaaaaaa ctaattgaac ttcttcagac acatatcata tgtacatggg
      361 acattagttc aagtgtttat catctaacac tgaagaactt atctgatgga ggtaaggagt
      421 ttgaaaaata tggtaacatt gctgctgcca ttttttcaga actgcgtgat gctgctgagt
      481 ctggtaatag aacaaatggc gatgttgaac ttctcgatcg atttggcaac cctattcgca
      541 aggggtacaa agttcccgga gcaaaatggg gggatggcac atctgctggt gaagcatcag
      601 atgcaatcaa agcattcgtg gcttcgcttg tatggctggc taatcgggca gtattttatt
      661 ctctcacagc taagtacctt caagcagata cttttttata tccattacgt caggcgtatc
      721 aacagacata tttaagccaa aactgtcagt atggttttga ctatgctaaa catattgtag
      781 aaaacttttc cacttcgtta agtcatgatc tcattgatat tcattctggt ggtaaagctg
      841 tagcaacagc aacttcgctt cctttatttt cggcatggct cgcaaaagaa actggtgatc
      901 caagttgtat tgttgaagct gcttataaca taagggacaa caacgaattt gtaagggccc
      961 gtgagaaaat gcgagaaata aagcgcttgt ttgatgaaaa tgaattgcct caagctaata
     1021 agtcagtaag agcattgctt actgaagtat cgaaagaatc agattatatt cgaactaagt
     1081 atgggctcaa gactagacaa ggtgtacctg taactaagct tgtacatgta tacaatactg
     1141 ttgcggctat taattccctt ccgaaactac cgacctttaa ttttaaagtt aaagttcctg
     1201 atttcttata tgatcttaaa aaacccaagg ggtttcgtgc tctctatcga aatttgacta
     1261 atgatctttc tactgtgtgg tcgttaggag aagctcgaga tattcttgga agtaaagttg
     1321 tgatagataa tcatgcaaga atttacagtc cgaagcatga agaaccgcgt ttcaaaaact
     1381 ctcattcccc atataagagc ccgatgtagc ttaagaaaaa gacacgccca ccttgtgcgt
     1441 ctacttctgg cacagagcgg actctcagat taggctttac tctgtgccat agatatgtaa
     1501 gctcacacca gagctcatac aacttattgc ggcatttccg gccattcagg atttgcagga
     1561 tccacacgac tgaccagaac actgtagcgt tcccatgcct ccagtcgact gcgctcctca
     1621 tctgttgcca tattcagcct gacagcgcgc tccagcggca aaatcacgga ttcagcatct
     1681 gcaagaagtc tggctttccg attttctgcc tgctgctgca attcctctgc cgtataaatt
     1741 cgtttaatca ctgtacagtc cttaaacatc cagttccctg aaatgtccgc ccgtcggtta
     1801 gcagtaatat ccggcacttc aacaacactt aatccatccg gtctgatagc tgtcacatcc
     1861 ttttccacat agcggataat attatctttg tcgtacgcta tttttatcgt gtcatcagca
     1921 aaatactttt gttcttcgta ccagttctta ccatcttctg aaaaaaacca gacaacatca
     1981 aagtcctttg tcaattgata ttgttcaacc gtttttggat tacctgccgt tatatttatc
     2041 aaatgctgca taaattatac ctgtgccacg ttataccaag tcccgttaat gtatttctgc
     2101 accggtctgt aatatacgcc accaatgtta tcggcagagt ttgagccggt atcctgaaca
     2161 ataatgccgg aatatacaca cccggacggt gcctgatgtg tccatgtcat accattgttc
     2221 gcaggtttgt atgtggcagc accaccaagc cggatatccc ggacatagcg tgaatcaaag
     2281 ttgccatagt tagatggtga tacctgcccg ttaacagcaa aagtgatgct gttatctgta
     2341 tttctctgac tgtaaaaatg ccagcctgca tcatcaccta attcagccac cacaggacga
     2401 cttgagtttc cccacaaatt gaatgcggct tccttcgtgg atgtattgct gctgctgacc
     2461 gtgaactttt tcccgctacc ggcacgtact ttggtacttg agacaatatc acctgtaaca
     2521 ctcaggccat gccccattga cactccgcca ttggcgttat tgatagtcag cggtcttaag
     2581 ccgttccatg tcccaaattt atcgccagag gccgtcagca ttaaatatgt gctgccgcca
     2641 tcattcctga taaagaatcc ataattgcca taagcaatgc gcagaccatt agcactgagt
     2701 gatgtaattt cacctcttga acggagacca taagcggagc tgagtgataa ttcttcctga
     2761 gcgtcattgt ttcctgtcgc ccagcgaatt accccgccct gaactgtttc atgccagata
     2821 gtatctcctt ctccaccacg aaactttctg agatattttt tgccgcctct ggtgcctgaa
     2881 cataaggccg tagacatata ggcattctgg cttccgccat cctgattaat cgttccggtc
     2941 attgagtcgc cctgacgatt ccagtcacga cgccagccgg gggagtagcc gtccccatga
     3001 tttatgtaag tgaattgcgc gctggttgta ccgccaccgc ttgatgttgt tggcgtggtc
     3061 actcggatag tgattgcaga tttagttccc atgacctcga cgacacaacc agccaggtgg
     3121 atatcaccac atccggtatc cgtaatgatt ttgttatttg catatgacca ggagcctttg
     3181 cacatccagt acggatgatt aaatgcacca cgggaatcca gccattcaat aaactgagcg
     3241 gttgtccagt ttccggcttc agtgctcaaa gcgccgctat aagcacgaca ggcaccgata
     3301 tttttcgtga aggtatcctt tcccggaata tccgcaccgt tctgatcttt ctgaagacgt
     3361 ttttcagcat tgtcataggc agacttcact gcttttggcg ttgcggccag cgtttcagaa
     3421 tcgctgttga tggcactact tagctggaca agaccttttc gcgctgtggt ggcatcctgt
     3481 gcagtgtatt tcccgttagc aaggtcatac gctgtcttaa ccgcctttgg tgttgccgca
     3541 agcgtttcag aatcgctgtt ggtggcgcta ctgagctgga caagaccttt tcgcgctgta
     3601 gtggcgtcct gtgcagtgta tttcccgtta gcaaggtcat acgctgtctt aaccgccttt
     3661 ggcgttgccg caagcgtttc agacgtgctg ttggtggcac tactgagctg gacaagacct
     3721 tttcgagctg tggtagcgtc ctgtgcggta tatttcccgt tagcaaggtc ataggcggcc
     3781 tttaccgctt tcggcgttgc ggccagtgtt tcagacgtgc tgttggtcgc actgcttaac
     3841 tgagtaaaac cttttgcggt cagcgaggcg tccgggtgac gtcgtgactg ttcgtgctct
     3901 gcaattttgt catcaacgta atcctgtgtc gccatcaccg ttgtggtgtc aatggtcagc
     3961 tccaccgagg ccacactgct gacgatgatg accatgcgac aggtctgcga acgccctgag
     4021 ccttcggcaa gagctggctt ataactttcg gccatgttcg ccacggcaat taacgttccc
     4081 gcatcatcgt acaggccaag ctcacgcatc cagaaaccgc ccacctccgg cggaataacc
     4141 agctctgcga taatataatt actgtttcgt ttgtcctggc tgattttgtt cagcgtatgt
     4201 cgccagactt cgtggataag cccggtctgt ccggcatccg ggacaggcaa tttaccaccg
     4261 ccatccccga cggccatcgt ggtaatgttg accttccgcc ctcccggtgc ggttgccgct
     4321 gccagctttg ctgcaccggc agtggtgata acggttctga attttgtgct cattattcct
     4381 cacttatccg gggtaaaccg taattacatc gccgtcgtaa gccacaccac cggcgaacag
     4441 gtagccggga atgtcccggg taatgttcag gccaataaga tgacggcttg caggtttggc
     4501 atcagcaata agccgttcca tttcctgata cattgcctct gtgatgccgc tttccagtac
     4561 accaatatca agccggaagg tgccgggcgg gtcactgttt tcccaccact ccgtcacgtt
     4621 gatgagatag ccgagcggct ccaccacacg ccggattgcg ccgatagtgc ctttatgaca
     4681 gtggatgaaa taggcatcgc ggataacggc gcgttttgtc gcttccggcc acttttcatc
     4741 ccacctgtcg accgaaaacg cccacgccag ccacggcagc agatttgccg gacaggtatc
     4801 cgggttccac agttcacgaa tcctgaccgg cgttttttca atttccgcac aggcttttgc
     4861 ggcggcaact tcaagcggtg atgagccggt cggcagcagt cgcgaatcac tcatccgagc
     4921 ctccggtcac gacgcggtat tcggtacaga aagacgcctg cgtactgttg agcacgatgt
     4981 cggccagcgg tgcagtcagt tcgacacgct gcacgccttc cacatgcaaa gcggcataaa
     5041 tggcagacag acggatgtcg cgccccagcc ggtgctgtgc cgtgatgtac gcttccagtt
     5101 ttttcacggc agcagcgcgt atgggttcgc tttcgggacc agggtaaagg taaagcgtgg
     5161 cgtttatctg gtattcaacg atggcggcag actgcacggt cacgcggtcg gccaccggcc
     5221 tgacgtcctc accattaagg gcgttacgca ccaccgccag caggtcttcg gatgcgacac
     5281 cgttattttc acgtgacagc acagagatgg tgacgcaggc cggagacgga ctggttacag
     5341 aaatatccgc gacacgcccg tcggcactgc gaccatgata ctgataggcc cccaccgacc
     5401 cggcgacgct taaaccttca aacgcctgct gaatacgcag acgataatcc gtgtcagatt
     5461 ccatcactgc cggtgtcggc gggagggtcg aatcatctgc cggggtgata atcaggcgcg
     5521 tggtgttgta attggcacca atcacatcaa ggtcattacc ggcggcacag gccagcatta
     5581 ccgcccgtgc ggcctcattc acacgctgac gccagataag ctcacgataa gcattttcct
     5641 ccagcagttt gacgagaggc tcggattcca gcgtcagggt acgggcgacc gcctcctgct
     5701 ggtcttccgg gtaaagggaa atcagtgtcg ccttgcgttc ggcaagaatg gtttcaaagt
     5761 ccagctcctc gaccacatcc ggtgcgggta gctggttcag gtcgataatc ggcatggttt
     5821 caactcacag ggatggttaa cgaaagtggc tggccggtgt cgttgtgctg gccggttaac
     5881 gtgaccgtca ttcgcccgtc aaagctgcgc gccgtggtga cggatgacag ggtgacgcgg
     5941 gcttcccatt tcagcacggc catgtaacag gcgaccttaa tctgcaactc aagcgccggg
     6001 gtctgcggct ggtcaatcat tgacgccagc aacgagccgt aatcacgacg catcacccgt
     6061 gagccgaccg gcgtgcgcag gatatcgccg atactctggc tgatatgctc aaggtcagag
     6121 acagtcaggc catcactgcg attcattccg agataacgcg ctgtcataga ggactcccgg
     6181 ttgtgccgcc gctgtcgccg gggtgtttat gggtatgcag taccttcccg tttgatgaga
     6241 gttcaccgcc ggtgtgttca atgttgccgc gcatcgtccc gcccttctgc acttccagcg
     6301 tgccagtgat cagcttgttt gtgcagacca cctccggcgt gtccagagtg acgcgggttg
     6361 atgctttcac cgtgaccacc ggcaccgtgg cagtaacaga atcagaagcc gtcacgctgg
     6421 ccgttttaat tccgcttacc gtgagtgcac tggtttcagg ttcatactca atcaccgccc
     6481 cgtcagggaa acggatatgc agggcatccg ccgacgcaga cggcgcgggg ttatcaccgg
     6541 aataaatccc cggcagaacg aacgccgtgt cgagttcacc gcctacggcc agaatcagca
     6601 cctgttcccc cacggaaggt gcccaccatg tgcgcgaacg tccggcgcga tgggtcagcc
     6661 actgaagcca gtcggtgcac atgccgccgg tctgcacacg gcagcgaccg gcattaaggt
     6721 cggtttcgac gataatgccg gtgcggatca tgttgcgcag tgcgcgcgcg agttcctgaa
     6781 tatttgcgag agtgttcata acgggaagga tgccgccggg tcataccggc ggcaatgtga
     6841 cgatgaggtg tcaggaatgg cacaactaac ggtcgaggtg agccaggata atctcttcaa
     6901 tcatctgcac atcctcaccg gtaaagccga gcaggggacg cgccggataa tgaattttct
     6961 taccgtcttt ccgggtttct tccgacagac cgaactgatg cacactggct attttcggtg
     7021 acttcccgcc gtaaaactcc attgatgcct gttcagggct ggcgcggata tgcaaaaaac
     7081 gactggtgat aagtttcgca aacatttttc gcttaacgcg accggtcttt tttctggcgc
     7141 tctgctgctg gcgtggtgcg tagggtgtgc cgtccggggc tttctgtgcc atcacccgac
     7201 gctgctgact ctgacgcaga cgtttcgcca gttcggcgct cagtcgccga cgccctgacg
     7261 gtgacagcga ctcaataagt ccggtcagcc tgtcttcaaa acgcttaaac tcattcatcc
     7321 cacttactca ccagttcgcc attgatataa agctccatcg ggcgggtgac cggctccggc
     7381 ggcggaggtt ccgggatatt cttcacatgc agcgcgccgt ccacctcact gaccagcgtg
     7441 cgctcggtca gcatcaggct gatgctgata tcaaagctgc tgtcattgtt gatgtctgca
     7501 taaaacgtga agcccttttt ctggccttcg tcggtggtca tgatgtcggg ctgattttcc
     7561 cgcagccacg ccagcaccgg cacgatgagc aggtcaaaat caccggtaaa gtcggtcaca
     7621 atcacgttga gcgtgtaacg cttttcgaat gacagcgacg tcgccagtgt ggaggcaata
     7681 ctcccgttat ccacgaatat ccgcagcatc tcggggcttg ttttcagcac cgtgacggca
     7741 tcagtcagcg ccctgcgcag gctgtcgggt ttgagcatcg ttttcgtcct gacagtgttt
     7801 aatcattttt acctggctgg cacagcgtgc cagcgcgttc tcaagctgcc ggatatcagc
     7861 acttaaatcg ccgttcgtct gcgggtcact gcccggcatc gggcaaaggc tcactttcgg
     7921 gcaggcgttg tggacaatca ctggcgtctg cgcaggctgt gcgctggtgc aaccggcgca
     7981 cagcatcagg caggtcagca ccgtaccagc ggcgaaaatc ttcgttttca ttaagtaacc
     8041 tcgtgatggt tttctcgcgc tgagcttcac gcttcgcggc gttctccagt tcctgacgca
     8101 gtgccacctg cgccagctcg tttttgtctg ccctggtgag ggcaacatga agctggtttt
     8161 tcagcatggt gatggtcgtc tgctgttcac tggcgacgtt gttcgccctg tccagcaagg
     8221 cgcgcaggct ggcatttttg tgtttcacca gaaacagacc ggccaccgcc agcgataaca
     8281 acacaaccag cacaatcatc agttttgaca tggttcccgc ccctcaagac gctgacggca
     8341 ggccgtacgt atcagccgga aaaacagcga tgccacgaga taaatcagtg cggtaaaaat
     8401 ccacccggca gcgaccagcg agataaacgt cgccaccatc accatcagag ccgctgcccg
     8461 tctgcgccac ggcaccggct gcaaaaacag cgacgtgaca atcttcacgg ccagcgattc
     8521 cggcggcagc tcccgcccgt agcgttccag cacatactcc gcggcataca cgccgacacc
     8581 accggcaatc acacagataa ccgtcgccag aatcgcccat gcagcgacaa aactgacggc
     8641 cacgctctgc gggtaaatca gggacagtgc cagcatcagc gccagcgaca cgttcagcat
     8701 cagtgaaagg gataatttct tcatggtgtt tactccgttt aagccggtac gccgccagcg
     8761 gtgcgccaga cggtgaccag tttttccagt gaatgctcac gctgaccgta accggcaccc
     8821 ggcagggacg cccagatatt gcgacagcgt gaaatggcgc gctcaatgcg tcccgcccgg
     8881 atgtcatcca gtgcaccgcg ttcgcggatc aactgaatgg cgagcctgtc ctgtgacaac
     8941 ggactgaaat ccggcagggc aagctgtttg cggtagtgcg gccagaacag gtaaagctgc
     9001 tgatagcgac cggaggccgt ggatttttca ccgcgacggt taaacacctt cgccggtcgg
     9061 ccatgtgcga acgggtggtc actgtagtcg gtgaagattt ccggcttccc gtccagtccg
     9121 gtgactatca cgtcatagcc ccggtttttc gtcagcggat gattcgccgt cccttcggac
     9181 acggccagca tgtcgagaaa ggcggcgata ttctgatgtg tgctaattac cggcattacg
     9241 gtttccccct gcccttaaag cggcgctgaa tggcaatctc aatcacctga taaccggcga
     9301 tacccagcat ggagccgatg ccgcacaccg caggcagtga caggtcagga aactgcacca
     9361 gaacaacacc ggcaaccatc gagacaaaac caccgagcaa catgcgcccg ataaacagac
     9421 gcggagtgat gggttcacca ccggcaagca ccttgccgac aacaatcagc accccaatca
     9481 tgaaaagcga caggacgctt ttttcttctg ctgtcatgcg ttactcccac agattgacag
     9541 tttcagccac gggcgcggtc tgaacgtcgg gcagttcgac ggcggtgccg tgtggcagca
     9601 ccgcccccag ttcagccagt cccggatttg cggcgagcac ggcctcgacc acgccctcag
     9661 tgcgcccgta ataccgaaca caaatggcgt cgagcgtgtc gccctgtagc gcaaaggtct
     9721 tcatcagatt tgactcacga tgcagcgcgg cttgtcctgg atacgcgcca ccgcccagcg
     9781 catatcccgc cacagctcat caatggtgct gtcaatgctg tcggccttct tgtcgccctt
     9841 ggcactggca tccacgccgc ggtaacgctc ataaagcgac gcggtcgcca tcgcacacac
     9901 agcgcgctcg tagtaaaaaa ctttgatgct ttcaccgtcg atgtcgtccg ccgggacgtc
     9961 agccagacgc gaaaaaccgg cagcaatttt ctgttcgcgg tactcgtaca gctccgcatt
    10021 cgtctccgcc atgcctgact tgatggcctc acgcagacgg gcgggggcga cggtctgctc
    10081 aaggcgcata cgttcccgga cgcgcttcgg gtcaatatcg ggaaaaaaga acgtgttttt
    10141 aatcaccggc tcgtcgcctg ccggttgcgg gataaccacc gtaccctcac cggacacggg
    10201 agcctccttt cgcggaataa tcagcgtcat catgactacc tctgaaaagt cgggcggtgg
    10261 acgccggtgc agtgtcaggt gattcaccgt cactgaccgg cgtgccgccc tggcgcgggg
    10321 cgcattcggt tgttaactgg ctttcttttt cgggcgtcca cgttttgccg gtgtcacgct
    10381 ccgggtctta cgcggagtgc gggtggccgc tttgggctgc ggctccggct tcggtttcag
    10441 ctcccgctcc agtcgttcaa tctctttttt gacgcctgcc tgacagtcga gctgtgtcgc
    10501 acgttgcagg tgtaccagcg cacctgcggc atcaccagcg tcacgcagaa acagaccggt
    10561 gattttgtgc agctttgcgc gcacttcatc aggcatgtca gccgtggcgg tcagttcgag
    10621 ggtgtccgtc agcaggcggg gatccacaga ctcaccggca gcgtgagcgc gcatggccgc
    10681 aagcgccacc tcctcggtga acatgtacgg cggggtgcgg cggtgtttac ccggcatggt
    10741 cagaccgtac ttcagggcat aacgggcaat ctccagcgca ccggcaatat cgccggtatc
    10801 cagacgccac agcatgaccg tcatcagaat gtcatcctgt gcacctttgc cctgctccag
    10861 cacgccgttc acccacggca accagaacgg cagcagttcg cgttttttcg cggccttcag
    10921 ctcttttgaa taaatcgctt tcagtgtgcg ctggtctgcg gccagcttga ccagcatctg
    10981 ctcatagaca gttgcatgtc gcagcggggc ggcttcccgc tgcgcggtca tcgctgccga
    11041 gacccgcatc atgtggcgct gtgcgggact cgtcatcggt tacgctcccg gctctgcggt
    11101 cgctttagcc ggtgtggaga aatcaccgac cttaattttt tccaccagac aaccggcggc
    11161 gtagtcctcc accacgtaat caatgttcat tgactcgtag ttctccacgc ggtcgagttt
    11221 cgggttttcc acaatcacgc ggcgatggct gtcatccatg tagtagatgg acaggttttc
    11281 cagcttcgtg atgagcatcg catccgccgg gaagtacggg acgcgtaccg ccggcaggtt
    11341 accgatgcgt ttctggctga tgatgacgtc agcggccagc atctcgctgt tatcctgctc
    11401 cttgttgacg atagggaaat acttgtccgc cagtagctga cgccccacaa tcaccacaag
    11461 gtcagggtct tcctgatacc aaggttcaat caggttgttg gtcgcatcca tcaccagtgc
    11521 atcgaggctt acataatcac cgcccttacc cacgcggata acctcagagg tggtgcggcc
    11581 ttcctcgtca gtgaccttgc tcatcacgcg cgccggggct tcattcggta tttctgcagc
    11641 cagccgaccg ccacatcctg cagcatcgga ttactgttgc ggtcagaggt ttcggcacgc
    11701 ttcacgccgt taaaaccggc catgatgaaa tcaagggact ggcgtttgat aatggcgtta
    11761 cggatacgga gctggaaatc ctgataacgc gcccacaggt caagcgtttt gtagcggata
    11821 taaaaatcga agttaacctg gtcgcattcg tacttgtttg acgccagctt cgagaagtcc
    11881 ttcggctgac gctcggtgcc accggcggtg tcggtggtgc tggcgatgga gccggtgaca
    11941 ccgatgccaa ttttttcccc tttcatttca ctgaccggca caatgttgat gcgggtcaga
    12001 aagtcagagg actcctgcat ggtgttcatc agggtctggg tgaccgacgg ttcaacggtg
    12061 aattttttcg acacatcacc ggcgtcgatg ccgttcagct cggcaacacg ggacaggtaa
    12121 gcattaaatt taaagcgggt ttcctggcgc atagtttttc ctgaaattaa gggttaatcg
    12181 tgaaggtttt cccggactga ctgacgccgg tcagcagttc gtcatcaggg cgtcaccgcc
    12241 accaccggtg gccttgctgc ggcgctgctg ggtcagactt tcggtgtggt cgagactgtt
    12301 tttcaggcgg gtgaatgcct ggctggtttc atccgccctg tcagtcacct cctgcttaag
    12361 tgcagaaaag gcggtttcca tctcagcgag gcgctgctca gtggcgctca gtttttcctg
    12421 cacatgttca gcaacagcgg tcaccgcttc atgcacgtca ttcagacggg catcatcgct
    12481 ggcctgtttg cggccaaaaa tggatttcac cttttcggtc agggcggtga acacggtttc
    12541 aggcaggtct tcaaattcca gctcaacagg cgttgccact gaaatcaggt tttcagggct
    12601 taatttgaag cggttcaggg ggttgtgttt tgccgtgcgg cagaattcca ggtattccgt
    12661 gccgaggctt gccgggtcat cggtgacggc cagacccacc agataacatt tgccggtgtt
    12721 ggcaaagttc ggctgaattt ccattgaggt gtagaccttc tgcgcggcct tgttcatcgc
    12781 gataaggtca tcggtcgggg tgattttcgc aaacagcgcc catttgcctt tcagcgccga
    12841 atcatcgtca atcttttcgg ccttcagttc ggccacatcg ccataacgct taaaaatacc
    12901 gtcaggcagg atgccgcgca gatgttccag gttaatgcgg caaccataga cacgcgggtc
    12961 aaaggtttcg gccatttcct gaatatcctg cgcactgatg acacgcccgt cacaggtgtc
    13021 accctcaacg ccgatacgaa agaattttga gacttttttt gccattgtca ggagtcctga
    13081 atagtgatta gaggagtcac atgtcggcat cagtttcccg acgatgcgca tcctccgcca
    13141 tcagtcccgg atggtttatc actgacacaa cagcacatta gcgaatcgcg gggcgcgact
    13201 cagtagcctt gccgtgtatt catcacggcg aggtattcat gaccatcacc acagacacca
    13261 ctcttttaca cgacccgcgt cgtcaggcgg cgctgctgta ctggcagggg ttttccgtgc
    13321 cgcagattgc cgccatgttg cagatgaaac gcccgacggt gcagagctgg aaacagcgcg
    13381 acggctggga cagtgttgcc cccatcagcc gtgtcgaaat gagtctggaa gcgcggctga
    13441 cccagctcat catcaaaccg cagaaaaccg gcggtgactt caaggaaatt gacctgctcg
    13501 gacgccagat tgaacgactg gcacgggtaa accgctacag ccagaccggc aacgaggcag
    13561 accttaatcc gaacatcgct aaccgcaaca aaggcgggcg tcgcaaaccg aaaaagaatt
    13621 ttttcagtga cgaggccatc gaaaagctgg agcagatttt ctttgagcag tctttcgact
    13681 atcagttgca ctggtatcgt gccgggcttg agcaccgcat ccgcgatatc ctgaaatccc
    13741 gccagattgg cgcgacgttt tatttttccc gcgaggcgct gctgcgcgcc ctgaaaaccg
    13801 gccataacca gatttttctg tcggccagta aaacgcaggc gtatgtgttc cgtgaataca
    13861 tcatcgcctt tgctcgtctg gttgacgttg acctgaccgg tgacccgatt gtcctgggca
    13921 ataacggcgc aaaactgatt tttctcggca ccaactccaa caccgcacag agccataacg
    13981 gcgacctgta cgtcgacgag attttctgga tcccgaattt tcaggtactg cgtaaggtgg
    14041 catcaggtat ggcctcacag agtcacctgc gctcgaccta tttctccacc ccgtccacgc
    14101 tggcgcacga cgcctacccg ttctggtcgg gtgaactgtt caaccgggga cgcgccagcg
    14161 ccgccgaacg tgtggaaatc gacgtcagtc ataacgccct tgccggtggt cttctctgtg
    14221 cggacggcca gtggcggcag attgtcacca ttgaggacgc cctgaaaggc ggctgcacat
    14281 tgttcgacat tgagcagctc aaacgcgaaa acagcgccga cgattttaaa aacctgttca
    14341 tgtgtgaatt tgttgacgac aaggtatcgg tgttcccgtt cgaggagctg caacgctgca
    14401 tggtcgacac gctggaagaa tgggaagact acgcgccgtt tgccgcaaat ccgttcggct
    14461 cccgcccggt atggattggt tacgacccgt cacaccgtgg cgacagcgcc ggatgcgtgg
    14521 tgctggcacc gccggtggtg gccggtggca aattcagaat acttgagcgt caccagtgga
    14581 aaggcatgga ctttgccacc caggcggagt ccatccgcaa actcaccgaa aaatacaacg
    14641 tcgaatacat cggtattgat gccaccggcc tcggtgtcgg cgtgttccag ctcgtgcgct
    14701 cgttctatcc cgccgcgcgc gatatccgct acacgccgga aatgaaaacc gcaatggtgc
    14761 tcaaggccaa agacgtcatc cgccgtggct gtctggaata tgacgtcagc gccaccgaca
    14821 tcaccagctc gttcatggct atccgcaaga ccatgaccag cagcggacgc agtgccacct
    14881 atgaggccag ccgcagcgag gaagccagcc acgccgacct cgcctgggca accatgcacg
    14941 ccctgttaaa tgagccactc accgccggta tcagcactcc gctgacatcc accattctgg
    15001 agttttactg atgagcaaga aaaaagggaa aacaccgcga cctgcggcaa aaacaatcac
    15061 cgccagcgcc ccgaaaatgg aagcattcac ctttggtgag ccggtgccgg tactcgaccg
    15121 ccgtgacatt ctggattacg tcgagtgcat cagtaacggc agatggtatg agccgccggt
    15181 cagctttacc ggtctggcaa aaagcctgcg tgctgccgtg catcacagct caccgattta
    15241 cgtcaaacgt aatattctgg cttcaacgtt tatcccgcac ccgtggcttt cccagcagga
    15301 tttcagccgt tttgtgctgg attttctggt gttcggtaat gcgtttctgg aaaagcgtta
    15361 cagcaccacc ggtaaggtca tcagactgga aacctcaccg gccaaatata cccgtcgtgg
    15421 cgtggaggag gatgtttact ggtgggtgcc gtccttcaac gagccgacag ccttcgcgcc
    15481 cggctccgtg tttcacctgc tggagcctga tattaatcag gagctgtacg gcctgccgga
    15541 atatctcagc gcccttaact ctgcatggct gaatgagtcg gccacgctgt tccgccgcaa
    15601 gtattacgaa aacggcgcac atgccggata catcatgtac gtcaccgatg ccgtgcagga
    15661 tcgcaacgat atcgaaatgc ttcgcgaaaa catggttaag tcgaaaggcc gcaataactt
    15721 taaaaatctg tttctctatg ccccacaggg gaaagccgac ggcattaaaa ttatcccgct
    15781 cagtgaagtg gcaacgaagg acgatttttt taatatcaaa aaagccagcg ccgctgacct
    15841 gctggacgcg caccgcatcc cctttcagtt gatggggggc aagccggaga acgtcgggtc
    15901 gctgggtgat attgagaaag tggcaaaggt ctttgtccgc aatgagctta tcccgttaca
    15961 ggacaggatc cgcgagataa acggctggct cggtcaggag gtcatccgct ttaaaaacta
    16021 ctcactggac actgacaacg gctgaacatc gccgcctgcg ggcggctttt ttacaccccg
    16081 ccatcacgcc ctcacacgct catcaccgca caaaaaatcc cgcagacaca ccaacgccca
    16141 acgggcagac taagcgccgt cacgacgcgc tgagacgctg gaaaataaaa tcagcaccac
    16201 cgccagcgcg cagtgctttc cccgcctcgc ccgcccgctt catggggcgg ttttaatgca
    16261 gttgcatgaa cactccaaga gcacgccagc gctagtgata actaacaata aaagcgactt
    16321 ctgacgcatg caaatccatg cacatgatgg catttttcat gatctatgcg gtatgctatc
    16381 cgcttcaatt tttaacactt g
//
LOCUS       DAZRUF010000080        16400 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZRUF010000080 DAZRUF010000000
VERSION     DAZRUF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.080"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            78..2411
                     /gene="bisC"
                     /locus_tag="ACHXOL_002064"
     CDS             78..2411
                     /gene="bisC"
                     /locus_tag="ACHXOL_002064"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR6809045.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2380..2820)
                     /locus_tag="ACHXOL_002065"
     CDS             complement(2380..2820)
                     /locus_tag="ACHXOL_002065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR6809046.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHXOL_002066"
     CDS             complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHXOL_002066"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR6809047.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3538..4236
                     /locus_tag="ACHXOL_002067"
     CDS             3538..4236
                     /locus_tag="ACHXOL_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR6809048.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4465..5673
                     /locus_tag="ACHXOL_002068"
     CDS             4465..5673
                     /locus_tag="ACHXOL_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6809049.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5997..7688
                     /gene="eptB"
                     /locus_tag="ACHXOL_002069"
     CDS             5997..7688
                     /gene="eptB"
                     /locus_tag="ACHXOL_002069"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR6809050.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7780..7856
                     /locus_tag="ACHXOL_002070"
     tRNA            7780..7856
                     /locus_tag="ACHXOL_002070"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7814..7816,aa:Pro,seq:cgg)
     gene            7891..8024
                     /locus_tag="ACHXOL_002071"
     ncRNA           7891..8024
                     /ncRNA_class="other"
                     /locus_tag="ACHXOL_002071"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8769..10376
                     /gene="dppA"
                     /locus_tag="ACHXOL_002072"
     CDS             8769..10376
                     /gene="dppA"
                     /locus_tag="ACHXOL_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR6809051.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(10439..10666)
                     /locus_tag="ACHXOL_002073"
     CDS             complement(10439..10666)
                     /locus_tag="ACHXOL_002073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809052.1"
                     /translation="MTRSLELRVCIAHKSIPALIGQGMKYCCRMALHAEAQRHRVYRL
                     LTKCALFMPDAALTPYPAYKIVQIQYIAEFS"
     gene            10684..11703
                     /gene="dppB"
                     /locus_tag="ACHXOL_002074"
     CDS             10684..11703
                     /gene="dppB"
                     /locus_tag="ACHXOL_002074"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR6809053.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11713..12615
                     /gene="dppC"
                     /locus_tag="ACHXOL_002075"
     CDS             11713..12615
                     /gene="dppC"
                     /locus_tag="ACHXOL_002075"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR6809054.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12626..13609
                     /gene="dppD"
                     /locus_tag="ACHXOL_002076"
     CDS             12626..13609
                     /gene="dppD"
                     /locus_tag="ACHXOL_002076"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR6809055.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13606..14610
                     /gene="dppF"
                     /locus_tag="ACHXOL_002077"
     CDS             13606..14610
                     /gene="dppF"
                     /locus_tag="ACHXOL_002077"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR6809056.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14640..15911)
                     /locus_tag="ACHXOL_002078"
     CDS             complement(14640..15911)
                     /locus_tag="ACHXOL_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR6809057.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3900 a         4093 c         4409 g         3998 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg cgttctgcgg
     1201 tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg
     1261 ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga
     1321 accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc
     1381 atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg
     1441 aatgcttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg actacctctt
     1501 ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga
     1561 agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga
     1621 gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat
     1681 ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc
     1741 cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca
     1801 gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa
     1861 ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt
     1921 gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac
     1981 agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt
     2041 ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg
     2101 ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga
     2161 tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag
     2221 gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg
     2281 tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc
     2341 tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg
     2401 ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg
     2461 aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat
     2521 cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac
     2581 ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc
     2641 gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg
     2701 caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt
     2761 actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat
     2821 ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag cccacatgcc
     2881 tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgct
     2941 ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt
     3001 gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg ttcgccgttc
     3061 tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg
     3121 cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc
     3181 ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt gaggacggtg
     3241 atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag ttttttactg
     3301 tcagtttcag gcacgcccca ttcattatca tggtaggcaa tataaagcgg gtcctgactc
     3361 acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc
     3421 gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata
     3481 tccttcatac aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg
     3541 atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt
     3601 gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc
     3661 ggacattcca cagagcgtta cacctgggat agtgatcatc aacctaatta caacgatatt
     3721 ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa
     3781 gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa
     3841 caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat
     3901 aacgagtttg gcgacagtac taccacgcag accgatccgt tatggcatgc cagcgtgagt
     3961 accttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc
     4021 tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg
     4081 gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat
     4141 caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa taatagcgac
     4201 tatctgtata cgatgggggt tagcgccaga ttttaacgta atagtcacaa ttgaaaccat
     4261 taaataacaa taattgtggc gatagtgggt gctaacttac caaataataa atttggtgaa
     4321 taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca
     4381 tgccgttttt ccccaggcat aaagtgcact tcgttatggt tgtcggcaga gatttttcct
     4441 ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg
     4501 acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac ctggagcctg
     4561 tttaatggcg cgctttccgc caagctggat gcgccggtaa gccaggtcgc tttctctttc
     4621 ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt acaggaacgt
     4681 tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg cttcttcctg
     4741 accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtctg
     4801 gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt
     4861 aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa
     4921 tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacatttgt gatttgggga
     4981 gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag
     5041 gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga gtcgatgcgt
     5101 aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac
     5161 gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca
     5221 gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt
     5281 ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg
     5341 ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc
     5401 tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc
     5461 ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt
     5521 agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac cttctacgtg
     5581 attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca gccagagcag
     5641 aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta ctgggcttca
     5701 gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt aaaatttggg
     5761 ccagttaact ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca
     5821 tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt
     5881 ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg
     5941 ggcctgaaaa gccgttaagc agggtgttat cacctgtttg tccagggttt gtttgcatga
     6001 gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc
     6061 tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg
     6121 tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt
     6181 tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg
     6241 tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg
     6301 gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga
     6361 actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc
     6421 gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg
     6481 cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc
     6541 agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga
     6601 actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg
     6661 atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca taccaggctc
     6721 cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc
     6781 atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc
     6841 tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt
     6901 ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt
     6961 tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt
     7021 ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc
     7081 cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc
     7141 tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta
     7201 actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg
     7261 atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg
     7321 atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg
     7381 cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac
     7441 tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg
     7501 aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac atgaaagtgc
     7561 cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg
     7621 atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa gaggcagcgg
     7681 ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt
     7741 gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcttggt
     7801 agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt
     7861 cgaaaagcct gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct
     7921 ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg
     7981 cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc
     8041 tcaacgagca ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcaggg
     8101 aggttcgact cgacgtaggc ctgataagac gcgccagcgt cgcatcaggc aacggcaccg
     8161 catttattgt cggatgcggc gcgaccgcct tattcgacca acacaccaca cattttcctg
     8221 ccaactttgc gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct
     8281 ttttttatat ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg
     8341 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
     8401 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
     8461 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
     8521 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
     8581 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
     8641 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
     8701 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
     8761 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
     8821 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
     8881 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
     8941 taccgcttta taaccgcctg gttgaattta aaatcggcac cactgaagtg atcccgggcc
     9001 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
     9061 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg tgaactgaac gccgatgacg
     9121 tggtgttctc gttcgaccgt cagaaaaacg cgcaaaaccc gtaccataaa gtctctggcg
     9181 gcagctacga atacttcgaa ggcatgggct tgccggagct gatcagtgaa gtgaaaaagg
     9241 tggacgacaa cacagttcag ttcgtactga ctcgcccgga agcgccgttc ctcgctgatc
     9301 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgatgcgatg atgaaagccg
     9361 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
     9421 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
     9481 agatcgatac gctggttttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
     9541 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
     9601 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcctata
     9661 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca ggcactgacc tacgcggtga
     9721 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
     9781 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
     9841 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
     9901 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
     9961 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    10021 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    10081 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    10141 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    10201 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcaga
    10261 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    10321 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    10381 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    10441 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    10501 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    10561 cctctgcgtg cagcgccatc cggcagcaat acttcattcc ctgtccgata agggcgggaa
    10621 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg
    10681 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    10741 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    10801 ggcgaacgcg ggatctcccc tgagcgtcac gcgcagctgc tggctgaact cggcttagat
    10861 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    10921 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    10981 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    11041 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    11101 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    11161 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    11221 ccgttaaccg gttttatgct gatcgacacc gccatctggg gtgaagatgg caactttatc
    11281 gacgccgttg cccatatgat cttaccggct attgtgctgg gtactatccc gctggcggtt
    11341 atcgttcgta tgacgcgctc ttcgatgctg gaagtgctgg gtgaagatta catccgcacc
    11401 gcgcgcgcca aagggttgac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    11461 atgctgccgg tagtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt
    11521 ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct ggttgattga cgcactgcaa
    11581 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    11641 gtcaacctgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    11701 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    11761 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg
    11821 gtttacatcg tcatcgtgct gtttatcacg atctttgcca actggattgc gccttataac
    11881 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagc
    11941 atggcgcact tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg gctgatgtac
    12001 ggcgcgcgct tgtcgctgct ggttggctgt ctggtggtgg tgttatcgct gattatgggc
    12061 gttattctcg gcctgatcgc cggttacttc ggcggcctgg tcgataacat catcatgcgc
    12121 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    12181 ttcggcccgt cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat
    12241 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt taccgcgtct
    12301 cgcgtggcag gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctg
    12361 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggccgcc
    12421 cttggcttcc tcggcatggg agcgcagccg ccaacaccag agtggggcac catgctctcc
    12481 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc
    12541 ctgctgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    12601 aaactgaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    12661 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    12721 gggcgaagtg gtcgggattg tgggtgagtc tggctccggt aagtcggtca gttcactggc
    12781 gattatgggg ctgattgatt atccgggccg cgtaatggcg gaaaagctgg tgtttaacgg
    12841 tcaggatttg cagcgtatat cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    12901 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    12961 catggaagcg attaaggtgc atcagggcgg taataagagc actcgccgtc agcgggcgat
    13021 tgacctgctg aatcaggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca
    13081 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    13141 aaaactgctg attgccgatg agccgaccac tgcgctggac gtgaccattc aggcgcaaat
    13201 catcgaactg ttgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    13261 tgatctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggccaggt
    13321 ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    13381 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    13441 tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    13501 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg acgggcgcca
    13561 gtccaaatgc cattacccac ttgatgatgc cgggaggccg acactatgag tacgcaagag
    13621 gccaccctgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    13681 aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg atggcgtttc gtttaacctt
    13741 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    13801 cggttgctga cgatgattga aacgcccacc ggtggcgagc tgtattacca ggggcaggat
    13861 ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    13921 cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg ggcaaattct tgaagagccg
    13981 ctgctgatca acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    14041 gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcatatgtt ctccggcggt
    14101 cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    14161 gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc aggtgctgaa tctgatgatg
    14221 gatttgcagc aggagttggg gctgtcttat gtctttatct cccacgacct gtcggtggtg
    14281 gagcacattg ctgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaagggaacg
    14341 aaagaccaaa tcttcaataa cccgcgccat ccgtacactc aggcgctact ttccgcgacg
    14401 ccgcgcctga acccggacga tcgccgcgag cgcatcaagc tcagcggtga actaccaagc
    14461 ccactgaatc caccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    14521 tgcacccagt tgcagccgca gctaaaagac tacggcggtc aactggtagc ttgttttgct
    14581 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    14641 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtacag cagaatcagc
    14701 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    14761 atgaggcacg acacaatacc atataacggg cttcccaact gaaagaacac cagcaccgat
    14821 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    14881 agcacgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    14941 cctttaatgg cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    15001 atattaagca ccgtagaggt gatatgaatg atatgcccag ggatcacctg cgcggccagc
    15061 gccagtgctg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    15121 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    15181 gtacgcagcg cgagccgggt tgccagtact ttatccgctt cccgtttacg ataggcaata
    15241 ttcattgggt taagtacctg aataaatact gcagaaaaga agcaaaatgg aatggtaagc
    15301 aagacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    15361 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    15421 ccggaaatct taaacagtaa tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa
    15481 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    15541 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    15601 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    15661 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    15721 actacccagg tggcaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    15781 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    15841 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    15901 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    15961 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    16021 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    16081 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    16141 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta aaatcagcgg
    16201 caagccgaaa gggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
    16261 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    16321 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    16381 ggctatatga cgctcgcaga
//
LOCUS       DAZRUF010000081        16272 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZRUF010000081 DAZRUF010000000
VERSION     DAZRUF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.081"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(164..979)
                     /gene="yahL"
                     /locus_tag="ACHXOL_002079"
     CDS             complement(164..979)
                     /gene="yahL"
                     /locus_tag="ACHXOL_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR6809058.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(1222..2271)
                     /gene="yahK"
                     /locus_tag="ACHXOL_002080"
     CDS             complement(1222..2271)
                     /gene="yahK"
                     /locus_tag="ACHXOL_002080"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR6809059.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(2358..2432)
                     /locus_tag="ACHXOL_002081"
                     /pseudo
     CDS             complement(2358..2432)
                     /locus_tag="ACHXOL_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2648..4030)
                     /locus_tag="ACHXOL_002082"
     CDS             complement(2648..4030)
                     /locus_tag="ACHXOL_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR6809060.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(4040..4990)
                     /locus_tag="ACHXOL_002083"
     CDS             complement(4040..4990)
                     /locus_tag="ACHXOL_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR6809061.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(5133..6551)
                     /locus_tag="ACHXOL_002084"
                     /pseudo
     CDS             complement(5133..6551)
                     /locus_tag="ACHXOL_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
     gene            complement(6551..8098)
                     /gene="fdrA"
                     /locus_tag="ACHXOL_002085"
     CDS             complement(6551..8098)
                     /gene="fdrA"
                     /locus_tag="ACHXOL_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR6809062.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(8088..8951)
                     /locus_tag="ACHXOL_002086"
     CDS             complement(8088..8951)
                     /locus_tag="ACHXOL_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR6809063.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(8991..9596)
                     /locus_tag="ACHXOL_002087"
     CDS             complement(8991..9596)
                     /locus_tag="ACHXOL_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR6809064.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDYTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            9854..10351
                     /locus_tag="ACHXOL_002088"
     CDS             9854..10351
                     /locus_tag="ACHXOL_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR6809065.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            10443..11375
                     /locus_tag="ACHXOL_002089"
     CDS             10443..11375
                     /locus_tag="ACHXOL_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR6809066.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(11417..12505)
                     /locus_tag="ACHXOL_002090"
     CDS             complement(11417..12505)
                     /locus_tag="ACHXOL_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR6809067.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(12647..>16272)
                     /gene="ehaA"
                     /locus_tag="ACHXOL_002091"
     CDS             complement(12647..>16272)
                     /gene="ehaA"
                     /locus_tag="ACHXOL_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFR6809068.1"
                     /translation="GFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIHVKGT
                     SRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGYYDQN
                     SDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKTSEKK
                     ITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGALGITQ
                     NIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYIDVASG
                     AVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFDEFAY
                     TDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGGTVDF
                     GALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDDGNSA
                     IKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTSGVNN
                     DGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQTVSL
                     SNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNGSADS
                     LLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSANVLVS
                     HAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAGNVLV
                     VKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLNGIEL
                     IHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDPMPNP
                     EPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYYT
                     DMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSDSWHV
                     GVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSWF
                     DNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGVK
                     ADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSKD
                     FSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGIK
                     WQF"
BASE COUNT         3775 a         4391 c         3854 g         4252 t
ORIGIN      
        1 actgctggtg cttttttaag cgaagtgtcg ccgatcacaa caaaatatcg gcgaaaaggc
       61 ggtggaatgc catttggcat acggatatac agatgaaatg attacctgct ttgcgattca
      121 ctctctggtg gaataacaac ttgcaggtgg aaccctttct gaactaaaga gtgtagcgga
      181 ttttaacgtg cttatttaca ggataatatc tgtcacctaa ttcctgcact ctattagata
      241 tccattcatt taatcgtgca tcatctctgt atgtttttac gtccagaaca gagagcaata
      301 acatcaccag tgattcagga aaaacagggt agatattttt taagtgtaat aataatccat
      361 gcgcaagcgc ctgcttaatg ggcgcgtctg tctccggaat agacgacatt tcgcgaagcc
      421 atttttccac atgcatacac tctttccctt tagggaaaag tctcagcaaa ggcatatggt
      481 gttcaagaag aatacatttg caatctgttc gcactgaatt gataaactgc actaatttct
      541 taagcccggt aaagcgtcca gcctgcacat ttgctttaat atcaagaaac gcatcatcct
      601 ggtcatatac ccaaactgtc cctttttgat tttcaagtat gcgaaaaaca aacttaaccg
      661 ggtgatcagg gtaatccatc cagtcataaa atcctgggaa atcattaaac tgcacattta
      721 tggtgagtgc gttatcgtct gaccactcac agtctcgtac ggcagccatg agatgaatga
      781 gtttgtcgtc atcgactaaa tcatcacgac aatcataagg ataatcataa tattgtttaa
      841 tcaaaacctt gaatatatcg acacaggaaa ccagggagct tttgatatcc tctggtaact
      901 gattgttttt aaccgtatta agtatgcttt gttgcgtata tggcatcaaa ttattgtgcg
      961 gagcttttaa tgatatcatc gcgatctctc ttaaataaaa ataaacgtcg cagataataa
     1021 agcgctgatt aaaccaggca ctatcagaaa tcgctcataa ctaacgtgag aagtattgtg
     1081 tactggaggg agttaattta acaatttgaa tttacgttat aggttttact gctactttga
     1141 tattatttcg ctctccttaa ctaaaaagtt aagtggagag cgaagttaat atgttaaacc
     1201 acagcgtatt tattaatttt ttcaatctgt tagtgtgcga ttatcgataa caaaacgata
     1261 tttcacatca ccacgcagca ttcgctcata ggcttcatta atttgatcgg cccgaatcat
     1321 ctctatatca gccacgatgc catgttcggc gcaaaaatcg agcatctcct gagtttctgg
     1381 aatgccgcca atcatagaat cggctatcgc acggcgtttc atgatcaggt tgaaaacttc
     1441 cggcgattta tgcggtgtcg caggcgcacc aaccagcgtc atggtgccat cacgcttcag
     1501 caaggtggta aaatcgtcga gattatgtgg cgcagctact gtattcaaaa tgaaatcgaa
     1561 actcttcaga tgagccgcca tctcatcggc attgcgtgag ttaacaactt catcggcccc
     1621 cagggctttt tccgcttcgc gttttgcctc agaagtggta aatgccacca catgtgcccc
     1681 catcgcgtgg gccagcttaa tccccatatg tcccagaccg ccgatgccga ccacgcccac
     1741 ttttttaccc ggcccggcct gccagtgacg tagcggcgag tacgtggtga tccctgcaca
     1801 caacaaagga gccaccgccg ccagctgctc ttgcgggtga cgaatacgca gaacatatcg
     1861 ctcatgaacg acgatctgtt gtgagtagcc gcccagagta tggcccggtt cgtccggcgt
     1921 cggcgagtta taggtgccga tcatgtgatc acagtagttt tccagcccgt cctcacactc
     1981 ttcgcaatgt ttacaactgt cgacaatgca gccgacaccg accagatcgc ccggcgcatg
     2041 tttttctacc tgatcaccaa cggctaccac acgccccaca atttcatgac ccggtacgca
     2101 ggggtaaacc gtccccgccc actcggaacg gacctggtgg agatcggaat ggcagacgcc
     2161 acagtaagcg atttcgattt tgacatcatt cggtcccggt tcacgccgtg tgatatccat
     2221 cggttcaagt ggttgtttag cggaatatgc accaacagct ttgatcttca ttgtgtttac
     2281 tcctgattag ctatgtgtat ttggcgacgc ctgatatgtc atcgcggcaa aacgcgtcca
     2341 ttgaatatag ccaatattta aaccacccga tccagcccca acatgccgcg aacctgaatg
     2401 aaacagagca gcaacgttcc ccataaggcg agcgcctata agtgtgatat ttgctatccg
     2461 taaaagacta aaatcggcaa aactgatgat cttttcacgt agacaaatag ttatctacac
     2521 aacgtcaata agggcatatt cctcaaagat aatgtccatt tctgccgaca ctatcatatt
     2581 gaggtcgccc cactccctgg ggcttaaatc tcttacaatt caccgttgca ggcgtttcaa
     2641 atgcgcatca acctgccaca ctcccccaca ccagttgccc tttatggaac gttgcggttc
     2701 tcggcgagat acgcgccacc gcctcggcgg aacaggaggc gtccaccagc acaaagctgg
     2761 catcatcctg cgcttttggc catacacgct cgcctttttc attcagcggc aatacatcgc
     2821 cagtggctag aaacagcgaa cgggagaggt tctgttcgtt aggacgaata tagagctgcg
     2881 cgtagagatt ggctttttcc agcatgtcgc ccagaccata aggcgaccag tggtcgataa
     2941 cgctgtcagt gccagtcatc acttttacgc ctttgtcgtg caactgtttg agcggcatat
     3001 gcagcgtgcc aatcggcacc gtcgaggcga tagaaatttg ctgcgccgcc atccgattcg
     3061 ccagcgcatc gacctgttgt tcgttgagcg tagccagcgc aaaggcgtgg ctgatggtca
     3121 gcttaccttt cagttgcggc gttttctcta ccgtttcaac catataattg atggctgcca
     3181 cgcccgacgg agtggtttcg tgcaggtgaa tatcgacacc tttgtcgtag tccagcgcaa
     3241 tctgaaacat ggtgtcgagg gatttttcca tcgcgccatc gacactggtc gggtccagcc
     3301 cgccgacgta atgcgcaccc gcctgcatcg cttcgcgcat taagggttcc gatttcgaca
     3361 gcagcaaacc gtgctgcggg aaggcgacga tttcacactc gaagcccgcc tgacgccgcg
     3421 ccagcaccgc ctgcaaattt tgcagatttt ttaggccgga aaccggttcg atattgcagt
     3481 ggctgcgggc aatggtggtg cctttcgact gcaatagatc gatcagtttt tccgcccgct
     3541 cctgcgtata cggttgtaac tccggcagca ttttctgctt gagtttgatc atgtcctgga
     3601 tagtggtgcc tgccggacga ttgagcgagc gccacggccc gccgtagaag gttttgtcga
     3661 gatgaatatg catgtcgcga gtggtgggta gcatcaactt accgccagcg tcatagtgcg
     3721 gcagcgtggc gtccggatgc tgcttgttct cgcgcagggc gacaattttg ccgtcctgaa
     3781 tctccacggt ctggcgcgcc gtgcgggtct gcaccgccac accgttttcg tagtcaaaac
     3841 cggtttccag cagcacgtta tcgagatagt aatgcgggtc agtgattttc atggtgttgt
     3901 tcgcttcgca gtttagggtg ccagctaccg ccgcatgggc gagcggcaca taggtaccaa
     3961 acagcgcggc ggcggtgacc atcttaccgc tctggctcag aaattcacgg cggctattgc
     4021 tttctttcat cctacgtcct tacgttttaa taatatgggt gcccgtttca ccgcgcagcg
     4081 ccgcaggcag gcattccggc gtggtgataa tcacttcttt gccgccctgc tccaggaacg
     4141 tcaggctggc gatgattttt ggcaacatgc tgccgggaga aaaatgccct tcctgcatat
     4201 agcgagtcat ggtggcaata tccacccgat ccagcgcctg ctgctgcggt ttaccaaagt
     4261 ggatacagac tttttcgacg tcggtggtga tcaccagaat gtcggcgtga atttcacggg
     4321 ccagcagcgc ggtagagaga tctttgtcaa taaccgcgtc cacgctttgg taatcccccg
     4381 cttcagtacg cactaccgga attccaccgc cgcccgcgcc aattacgaca aaaccttgtt
     4441 ggatcagcgc cttaatggcg ggtgcttcga caatacgttt cggttccggc gaggcgacca
     4501 cgcgacgata gccccgtccg gcatcttcaa caaaacacca gtcagggttt gccttttgta
     4561 gtttgtcacg ctggctttca ctgaagaatg caccaatggg cttggtggga tgggcaaaac
     4621 ctggatcgtt tttatccact tccacctgag ttaccacggt gacggctttt ttctcgccgt
     4681 gacgcgccag tcggttattc agcgcctgtt ggatcagata gccgatgccg ccctgcgtat
     4741 ctgccacaca gttcgccagc ggcgttaagg gcagcccttc gcgctcgtgg gcaatctccg
     4801 cacggcgtaa atccagcccg acctgcggcc cgttaccgtg ggtcagcaca atgtcgtaat
     4861 cggaagccag catttccagc accgtatcgg ccacggcttt caccgcctcc gcctgatgct
     4921 caatcgactg gctggcgtta tctttgataa tgctgttgcc accaatggca acgaccacaa
     4981 gctctttcat gttgttatgt ccgctacaga gggtgatgag gttatgacca acgccagact
     5041 gcctgatgcg ctacgcttat caggcctaca attctcgggc catccgtagg ccggataagg
     5101 cgttcacgcc gcatccggca taaacatgta agtcagacgc cgtaacgttc gcaccagccg
     5161 agaatggctt tctcgaagca agcgaatggt ggatgcacaa tgcccgcgcc aatcatgccg
     5221 acgcccgcat ctttatgggc gatggcggtg ttgatcaccg gcagaatgcc gctgctaccc
     5281 acgcgggtga tgtcgattgc cgacggcacg cccataaaac cgagcagcgg aatggtgacg
     5341 ttggggtttt caccgagagt gatttcacgc atctgacggg agaaatcgat agcctcttcc
     5401 accgtgccgc ccaccagcgc gacgattgca ggcgcggtcg ccatagcaaa tccaccaata
     5461 ccgtaggttt cggtgatggc gctgtcgccg atatccagcc ccgaatcttc cggcttataa
     5521 ccggcaaaca tcgggccgat cacctgctgc gccgggccgg taaaccattg ccccggcagg
     5581 ccgctgaccc gcaggccgaa ctcgacgccg ttacgcgcca tggtggtgac cacggtgctg
     5641 tattcgatgc cgtgcgccgc atccatcgcc gctttacaca tcgccatcca cgtcgggccg
     5701 gagaagtagt cgctgctggc gacaaactcg aacacttcgc gctgttgctc gacggaatag
     5761 cctgcctgaa tgatccccgg cgtcagtgcc tgaatcagca gcgtcgtccc ggcgttattg
     5821 cggttatggc actcatcgcc catatgcagc gcctgcgcca gcattaagcg cagatcgatc
     5881 tcaccgataa ttttcatcgc gtcgcgcagc attggcccct gcacatcgcg catccagttg
     5941 aggcggtcga tcacgctctg atcgttggca cccatacgca aaatcttcgc catctgctcg
     6001 ctcatattgg tgtaagcgat gttgccgtag gttttgtttt tcacgatgtg cataaacatc
     6061 gaggccgagg taacgcccgc catcgatccc acacagtcgt gttcgtggca cggcgagaag
     6121 gtgatctccc cggaggccgc cagttcagcc gcctcgtcga gatctttcgc cagtccttcg
     6181 aacaccagcg ctccggtgac cgcgcctttc atcgcgccgc acattttttc ctaggtgatc
     6241 ggcggcccgg cgtgaagaat ggttttcggc gtcatgcccg gcaccacgtt aatcgcctga
     6301 tcaaaaccaa tcagcaccgg atgcgactgg ataatgcgct ccagcgcctg ctggttagcg
     6361 gcggcgattt tgtctgccag cggcgaatcg gcaatgttat ccagcgcctg caccacctgc
     6421 atattgccct gccccggcgg cgtccagtcg agttgagtca cttctacatg ctgctttttc
     6481 aggtcatcgc taaacatggc gatgccgacg ttaataacgt tcaatggttg gctaaacagt
     6541 gactggctca tcaggcttcc tcccctttgc agataaattc acgcgccagc aatccggtat
     6601 tggtgctgct gctcgccaga atcactccgg catcaagcag catctggctt tgttgttcta
     6661 acgatggcgt atcaagatcg gtacccagca cataggcgag aatgatcaac tcgcgtcctt
     6721 cggcagcggc gatcgctttc gcttctttga tcgcctcgat ggtggagccg accggatctt
     6781 catgcgatcc aaatccgagc acaaaatcca tcacgatcac cgccacttct ggatcgcgcg
     6841 cctcttcgat caagcgactg atgcggttgg tggggtcaat cattggatgc ggcttgccgt
     6901 tcgtgaagtc gtcatcgcca aagtcgagga aggtgtgttt gatgctgcgg ttgatatctt
     6961 gcaggcggaa ttccggatcg ggctgaatgt tgctgtagac atcgccatgt ttttccatca
     7021 ccgcgaacat ggtttcgtcg cacagcgtgc cgccgcagaa caggccacga atgtatttct
     7081 gctgcggttg cagacgcgca cgcacatcag caatcaacgg ctggttaagc gtatgcaggt
     7141 cgagattttc ctgtttcacg ccggagagca tcaccgcttt tagcgccgcc tctttgctgc
     7201 cgcgggcaaa ctgtagcccc tgctcatcca ccggcgtttc aacgcgaccg aggaagcaga
     7261 ccaccaccgg cttgcggcag gcacgtgcgc gttccagcac tttgcgggcc accgcaggcg
     7321 cgggaggttt tgaaatcagt gcgataattt cagtttgcgg atcgttttcc agcatcccga
     7381 tggcgtcgag catcatcagg ccgccgattt tctcgctcag gtcgcgcccg ccggtgccaa
     7441 tcagttgtga aacaccgcca ccaaattcat gaatgcggac gctcagctcc tggctgccgg
     7501 tgccggatgc gccaacaata ccgatattgc cgcgacgcac ggcgttacca aaacagagcg
     7561 ccgcgccgtt aataatcgcc gtgccgcagt ccggccccat catcagcagc cctttttcgt
     7621 gggccaactg cttgagcgcc agttcatctt caactgagac gttatcggaa aacagcatca
     7681 cgttgagatc gttttgcagc gcctgacgcg cttcgcgagc ggcaaacaga ccgttgaccg
     7741 aaatcaccgc caggttactt tccgggatat gctttttggc gctggcaata gtggcgtaac
     7801 gcgcctcgtg cgagccgctt tgcgctttgg tgttgaacag ttcttcaatc tccaccagca
     7861 actgctcgtt gtccgcaccc gatttaccat tgatgacaat catcaggtcg ccgtttttcg
     7921 cctgttccag cgtcggcgtc agcagtccta aatttttcag cacacctttg ttcatttcgg
     7981 tcgccatcgc cacaaatgcc tgctcgacgc cgtcgagttt atttgcacgc gtggagatag
     8041 acatcagcga gacagaatca aaataggtat tcggtttaat gactatttta actgacataa
     8101 ttccctccgt aggtttggct cagcgcacaa ccatcggcca tgccatacag catgtcgcag
     8161 ccggaactgg agccaatatg tttaattttt tctattgcct gagttgaatt cccagggacg
     8221 ccataaataa tgttgtgaat aaagcgatga atattttccc ggcagcgttg ttgtaatgcg
     8281 gcttccagcg ttatggcact taataacgtg gtgttatttc tgccgcgtag cagaccgcga
     8341 taaaattcct ctttgtattt ttctgccgga tgcccgggaa taaataaaat aagcgccaga
     8401 cctgttaaat aatcatcggc tgatggcgta aggccaatcc ctaacccaat catactggcg
     8461 accgctgcgg cgatattttg cttatcgttt actgtctgaa taactgcccg tcgtttaata
     8521 tgtaattctt tatttaacgc ctgataaaac ggattatcgc cgcgataatt aaacagggtt
     8581 tcattttccc ccaggtgctg atgaataatg tcgtgccact gtagccaggc gataaggtga
     8641 aaattcacag cggtcatttg caaggttggg gacagccaac gccgacaacg actcatctct
     8701 atatgaagat gttgaccaac cgtaatacct tgatcgtcaa actgaacctt atcaccatgc
     8761 cggaacagat catcaaagtg agtgagtgcc aaccgacaac tgtttggcgc attgtcgtac
     8821 tcttcacaaa gcagcgtcag caactgctgg cgagccggga gtgcgatatt taccgctctg
     8881 gcaaagacct gttcaacctg tccttgcccc cgctgcgcca gaaaatccgc atccgcagtt
     8941 agcgcccaca cacaccgttt ttctggtgtg ttgatagcca gcctcccggt ttatgcacct
     9001 gcggcaatca gtaactgcgc aatctcttca aagccccgtt cccgcgccag ttccagtggc
     9061 gttttgccat atttatcggt cagatgcggg ctggcaccgt gttccagcaa taactgcaca
     9121 atcgcctgct gtttaatacc accatcatta agcacaatcg cttccagtag cggcgtccag
     9181 cctacatggt tggtctggtt aacgttaatc tccgtatgcg ccaaaagctc ttttacaata
     9241 cttaaatggc ctttttcaca ggcaggcgtc aggccgacac cgccaaaacg ggttacgcaa
     9301 ttaagatcgg gtttagccgg taaaataatt cgtagtagcg ttaaatcatc gttcagacag
     9361 ctaattaaaa aaggatttaa acaggtataa tcttgcttat taatatccgc tccggcatca
     9421 attaatgcct gaacgcaagc atattgctga tataaacttg ccagcgtaat tgccgttttc
     9481 ccctgacgat cgcaggtatt aatatcgaca cccagcgcaa ggcaggtttt tactttatcg
     9541 atatcaccct gttgtgcagc caataaatag tcggcaggta gattttttat actcattcac
     9601 attcccctgg tttttgtggc tgttttcgat gaattgagaa tagcaacgat aatctgataa
     9661 aagaacccgc ttaaatttaa ttcgccgtat ctcgcctttt tgttgaataa tattcaacaa
     9721 tcttttcgca gtgtgcggta gtgcaatatt tgtttctttg acccgatacc ctttcactca
     9781 atcaggtttt atttctctat agtctgcaca tgtaagagta gctttcgtcc atgtctaacc
     9841 agaggggaac aagatgaacg gactcacggc aacgggtgtc accgtcggta tttgtgcagg
     9901 gctttggcag ttagtgtctt cacatgtcgg gctctctcaa ggatgggaat tattaggaac
     9961 cattggcttc gtcgcctttt gcagttttta tgccgcaggc ggcggtaaat cggggtttat
    10021 cagaagtctg gcggttaatt attccgggat ggtgtgggcg tttttcgccg cgctgactgc
    10081 tggttggctg gcctctgtaa gtggactgtc cgcgttctgg gcgagcgtaa ttactaccgt
    10141 acccttctcg gcggtagtgg tctggcaagg gcgtttctgg ttactgtcct ttattcccgg
    10201 tggctttctc ggcatgacgc tgttttttgc cagcgggatg aactggacgg tgacgttact
    10261 gggttttctg gcgggtaact gcgtggggat aatttcagaa tatggtggac aaaaattaag
    10321 cgaagcgacg actaaacgcg acgggtatta aaagttattc aaaaatgatt atctatcttt
    10381 aaccgatacc tctacagtca tagctggaga aattcatagt agataatggc gagaatggtt
    10441 taatgaactc aatttttacc gaagagaatc tgttggcatt tactaccgct gcgcgttttg
    10501 gcagcttcag taaagccgca gaagagttgg gtttaaccac ttccgccatt agctacacca
    10561 tcaagcgtat ggagacgggg ctggatgtgg tgctgttcac tcgcagtacc cgcagcattg
    10621 agttaacgga gtccgggcgt tattttttcc gcaaagctac cgacctgctg aatgattttt
    10681 atgccatcaa acgcagtatc gatactattt cgcagggcat agaagcgcgt gtgcgcattt
    10741 gtattaatca gctgctttat acgcccaaac acaccgcacg cttgttacag gtgctgaaaa
    10801 agcagtttcc tacctgccag attaccgtca ccaccgaagt gtataacggc gtctgggatg
    10861 cgattattaa taatcaggcc aatatcgcca tcggcgcacc cgatacacta ctggacggcg
    10921 gcggtattga ttacactgaa attggcgcga ttcgctgggc ttttgctatc gccccggatc
    10981 acccgttagc ctttgtgccg gaacccatcg ccgaaagcca actgcgtctc taccctaata
    11041 ttatggtgga ggacaccgcg catacgatta ataaaaaagt gggctggttg ctgcacgggc
    11101 aggagtcaat tctggtgcca gatttcaaca ccaaatgtca gtgccagatt ttaggtgaag
    11161 gaattggttt tttgccggat tacatggtcc gtgaggcgat gacgcaatcc ctgctggtta
    11221 cccgacaaat ccataatccg cgccaggatt cgcgcatgtt actggcgact cagcattctg
    11281 ctacgggtca ggtgacgcag tggattaaaa aacaatttgc gccaaatggc atattaacgg
    11341 ggatctatca ggatttactg catcgggaga attaatcgct gccaacctcc cgatgcgctg
    11401 tgaatagcgg tttacatcaa ccacctgctt tcattaccca ttcagagata aatttattac
    11461 ccggtactgg cggagagtac aaatagccct gcaaaaagtg aacgccttta ccgatcatta
    11521 aatccgcctg ctcctgggtt tctacccctt ccgccacgat actcagacca ggcttacgcg
    11581 ctagttcgac aatattgtcc acaatatgac cggagatttc gtcgacactc gccatttgca
    11641 caaatgactt atcgatctta ataaaatcga ccgggaacgc ctgcaagtaa cgataggtcg
    11701 cataacccgt accaaagtca tccagcgcaa aggtaatgtt gtgctgatga aggctgtcaa
    11761 atatcgctct ggcttctggc gttaccggaa tagggttacg ttccgttagc tcgagaacca
    11821 gcttgatttt atcgttacct aatttcttaa ccaggttcag acactctttt tcaaatcccg
    11881 ctgccaaaaa acaacccgcc gagacgttga tgccaatatg gaaattgtcc ggcagcaaat
    11941 gttttaccgg catcagaata tccgcagtct gtttcatcag ttggcgggtc attatgacaa
    12001 taagaccgga tgactccgcc agaggaataa actgatccgg tgggataatt cccgtttgtg
    12061 gatgttccca gcggacaagt acctcacagc ccgtcagtac cccagtctgc gcgcagaaaa
    12121 ccggttggat ccacggtttg aattcgtggt tttccaacgc cagactgatg gcttcaggcg
    12181 tgacgatatg gtgacaacgg ttatcaggga tataaaaatt atttttattt cccgtgccac
    12241 tgatgacttg cggatgatat tgaaaaaaga tatcccgcca gaaggtaata tcactctgga
    12301 tgcctagttt ttcatacagc tgcttctttt gatgtgaaat tgtctttgca ctgcgatttc
    12361 tgtactgtga gatttctgta accgacattc cctccattag cagcttgagt aaaagccgtt
    12421 ctttttcgga cacgatacca ggcaacgtca aattcaccgt tgccccgaac tcttccaaaa
    12481 aaacactcaa atcatgcgaa gccattgaac acaccttgat taaatcttgt gcgccagcta
    12541 aatctgcgag tcttcactct gactgataaa gctggctgca agaaaatgac acagagagcc
    12601 gaattatctc ccgttcggct ctctctcctt ttactcatta ttcagatcag aattgccact
    12661 taatgccaac cattgcagag gtgtcattat atcccctatc ggcaacctga acgcccacat
    12721 tcccccagac attcaggttg gcatttagct gtccttccac cccggttttt atctcagcaa
    12781 tatttcgggc tccatcctga gtgacggaca cgccatccat actggtgctg aaatccttac
    12841 tgttatgcag ccagttcact tctacaaacg gctggaactc gcgggattta ccgtcatcca
    12901 ttttatggtg gctcttcagc caggttttta cgccaagtcg ggtttgaaca ttgccatcac
    12961 cgttgctatg aacgagggtt ccgttgcttt cgcggtgctt atcggctttg actcccatcc
    13021 aggtaacctg tgcttgcggc tgaacatacc attcattacg cgttccctgg ctgccattaa
    13081 attcagctaa tttgtgtttg tatccagctt ccagtgaagc ggtaaatcct tttgatttat
    13141 aggattcact ttgtaagtca tcccctttca ctgtgttatc aaaccagctg tactgcgccc
    13201 aactgtcgag atacgcgcca ttacgcgact cgtcatcggc ataccaggtg gcatagaggc
    13261 cggtgctata tccgttcaca ctcgcttttg cacgataacc ggttcgcgag gaaatggttt
    13321 tgctgtcgct gttgccatat cccgccatga ccccaacatg ccagctgtcg ctgccgtttt
    13381 ggctccactg cgcgacatcg cctcccagtt gcagaacata gcgattgctt tgggttttca
    13441 gctggccgct gccatcacgc catttattat gaccaccttc atggcgcatc cacatagtgg
    13501 tttgtttctg ctcacccgtc accatgtcgg tatagtacgt attacccaga cgctcatgca
    13561 gacgcgtggt gaacattgta ttcgctgctg caaggttcgc aatgtagcta cccgcctccg
    13621 gtcgcaggtc attatccaca ttcagatccg gacccggcgt aggtgtcggg ttagggttcg
    13681 gctctggatt cgggtttggc tccggattcg gcatcgggtc tggctccggc tgcagttcag
    13741 gagaatcact gccgctggtc agataccagt taccactatt tgccccttgt ccacgcgcga
    13801 gagtgtagtc atacgccccc gcaacgatac gaccagcctg aacaaattcg ccctcagact
    13861 taccgtctac atggataagt tcgataccgt taagggtttt cgcacctgta ccgccagcgt
    13921 tattcaccgt aacggcagtc gtgccgctag tatcgccctc gacaaccaat ttatcggtta
    13981 ctgagtcatc gccattcagt accgtattca ttactagttg accgttatta ccgtggtagt
    14041 tccccttcac cactaacaca ttgccagctt gctgtcctga cattccggtc atcagcgtac
    14101 cgttgttggt cagattgccg cccagggtgt aattcacaga cgcagccgct cttttttcag
    14161 cgctattatt caacgccagg gtaccgttgt tctcaacgtt tgcgctaccc aatccctgcg
    14221 catgacttac caggacatta gccgaattag caatgttcac attcgctgtc aggttgctgt
    14281 tatcgcccgc gatgtccagc gtgccgccct gaatattcag ctctccgctc cccgttaacg
    14341 aaccggttga agtgcccccg ttggtaaccg tcagactgcc accatttaag ctcagcagtg
    14401 aatcggcgct gccattgagc gtgcccacgg tctggctgtg accattcata tccagttcag
    14461 tctctgccgc cagacgcagt tcatgggtat tacccaacac gttatcgtta ttcaacaaaa
    14521 gcgtcccact gcgcagatcg gtaacaccgg tatagtcgtt gtctttgtta gaaagcgata
    14581 cggtctgacc cttctggctg ctgaatgcca ggtcaccact gccggtaatc tttgcgctga
    14641 gatcggcggc attctcgctt ttaccgttag agctcagcac cagagcatcg ctgtcggtag
    14701 cgtgtaaatc aagctgggtc aggccgtaac cgatatacag accgtcattg tttaccccac
    14761 tcgtcagacg atagtcgtaa gtgccgacgg ccgctttttg cccattctgc tgaatatcac
    14821 gttgggcact gctggagagg atttgcccat ttttatcctg caattgcaga ttgcccgcat
    14881 cgcccagaac ttccgcacct tgcgcatcaa ccagcttaat ggcgctgtta ccatcatcga
    14941 cttcagtgag cgataacgca gagtcaatat cacgagatac tgagcggaca acgtcactgt
    15001 cagaaacctg aatcacaccc tcgccgcgca aatccagcgt tttactaacg ttgaccgtcc
    15061 cttctgtcat ctgtgcacct gctgtcaggg caccaaaatc aacggttccg ccggagaatc
    15121 ccaaagtgct aatggagtga gcgcccttac ccactgttac caggcttccc tgacccgcag
    15181 tgatcccgcc tctctgcaga aactcagcat tcttttccag gttaaatgtc gtgttctgga
    15241 acgccagtga accctggaac cagtctgtat aagcaaactc atcgaaggag aattcgtgat
    15301 tccccatgtt tatttcaaca atacctctac cttgaagatc gttatcaaat acccaatctt
    15361 tattggcatt gatttgcagt ttgccatcaa catccaggtc cgcacggccg atgttcttgt
    15421 cttcagaaat aaccgcgaca gcaccagaag cgacatcaat atatccgctg aatgcatagt
    15481 tattgccggt cagcgccact gtcccactgt cgatacttat tgtaccgttg ccggaaatct
    15541 tattggcaaa ggtcatgcta tcgtcagcct tcaggtgtaa tttcccgcgg ttgttgattt
    15601 ctgcggtacc tatattttgg gtgatcccta aagcgccatt ttgctcaatt ttgactttac
    15661 cgctaaaggc ggagttatcc ccggttaagt aagtcgtacc attgagcgca ttgatatagc
    15721 ccaggccctc gctgtcactg acaatatccg cggaaatagt cgcgttttta tccgtgtggt
    15781 ttaaggtgat tttcttctcg gatgtctttg cccacacaaa ctcaattttt tcggcatcaa
    15841 taatccctgc agccttcgct gcgcttcctt cctgtgcacc taacgctaac tcagagttgg
    15901 tgcttaaact aatagtcggc gcagagattt taccaccttc cgaaacagca agtgttgtat
    15961 cggagttttg atcataataa cccaggctaa ttttctcact ggcattaact tcaccgccgt
    16021 tattaactga caggtactga ttaccaccgt aactgccgta aacgcccata aagaaggttt
    16081 tagaatttac ctggctgcct tcaccactga catttccctg caaataggtc agacggctgg
    16141 tgcccttaac atggattttg ccgccgttgg agacattaaa atagccgcga tcgtgtctca
    16201 tgcctgagtt tgactcatac aaatcatcag catataactc gctgccagca ttggagacat
    16261 tcaggaaccc cg
//
LOCUS       DAZRUF010000082        15955 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZRUF010000082 DAZRUF010000000
VERSION     DAZRUF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.082"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            111..713
                     /gene="ais"
                     /locus_tag="ACHXOL_002092"
     CDS             111..713
                     /gene="ais"
                     /locus_tag="ACHXOL_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR6809069.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(752..1177)
                     /gene="nudI"
                     /locus_tag="ACHXOL_002093"
     CDS             complement(752..1177)
                     /gene="nudI"
                     /locus_tag="ACHXOL_002093"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR6809070.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1456..1998
                     /locus_tag="ACHXOL_002094"
     CDS             1456..1998
                     /locus_tag="ACHXOL_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR6809071.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2098..3300
                     /locus_tag="ACHXOL_002095"
     CDS             2098..3300
                     /locus_tag="ACHXOL_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR6809072.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3520..4302
                     /locus_tag="ACHXOL_002096"
     CDS             3520..4302
                     /locus_tag="ACHXOL_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR6809073.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4317..5522
                     /gene="rhmD"
                     /locus_tag="ACHXOL_002097"
     CDS             4317..5522
                     /gene="rhmD"
                     /locus_tag="ACHXOL_002097"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFR6809074.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAVMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5579..6868
                     /locus_tag="ACHXOL_002098"
     CDS             5579..6868
                     /locus_tag="ACHXOL_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6809075.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6886..7689
                     /gene="yfaU"
                     /locus_tag="ACHXOL_002099"
     CDS             6886..7689
                     /gene="yfaU"
                     /locus_tag="ACHXOL_002099"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR6809076.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7730..8632)
                     /locus_tag="ACHXOL_002100"
     CDS             complement(7730..8632)
                     /locus_tag="ACHXOL_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR6809077.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8825..10015)
                     /gene="glpC"
                     /locus_tag="ACHXOL_002101"
     CDS             complement(8825..10015)
                     /gene="glpC"
                     /locus_tag="ACHXOL_002101"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR6809078.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10012..11271)
                     /gene="glpB"
                     /locus_tag="ACHXOL_002102"
     CDS             complement(10012..11271)
                     /gene="glpB"
                     /locus_tag="ACHXOL_002102"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR6809079.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11261..12889)
                     /gene="glpA"
                     /locus_tag="ACHXOL_002103"
     CDS             complement(11261..12889)
                     /gene="glpA"
                     /locus_tag="ACHXOL_002103"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR6809080.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13162..14520
                     /gene="glpT"
                     /locus_tag="ACHXOL_002104"
     CDS             13162..14520
                     /gene="glpT"
                     /locus_tag="ACHXOL_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR6809081.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            14525..15601
                     /gene="glpQ"
                     /locus_tag="ACHXOL_002105"
     CDS             14525..15601
                     /gene="glpQ"
                     /locus_tag="ACHXOL_002105"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR6809082.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(15643..15885)
                     /locus_tag="ACHXOL_002106"
                     /pseudo
     CDS             complement(15643..15885)
                     /locus_tag="ACHXOL_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         3708 a         3888 c         4301 g         4058 t
ORIGIN      
        1 tgccgacatc gtaggatttt taatattaca ccaactgcta attatcgcca gaaatgtcgc
       61 tcaatttcac ggtaattgtc tggttgcgct tgtctatagg tggagtttac gtgttagctt
      121 tttgccgctc atcattgaag tcaaaaaaat atttcatcat tttactggcg ctcgctgcaa
      181 ttgccggact gggtactcat gccgcctgga gtagcaatgg tttgccacgt atcgacaata
      241 aaacattggc cagactggca cagcagcacc cggttgtcgt tttgtttcgc catgctgaac
      301 gttgcgatcg ttcaaccaat caatgcttgt cagataaaac aggtattacg gttaaaggta
      361 cccaggatgc ccgtgaactg ggcaacgctt ttagcgctga catccctgat ttcgatcttt
      421 attccagtaa taccgtccgg accattcagt cggcgacctg gttttccgcg ggtaaaaaac
      481 tgacggtaga taaacgactt cttcagtgcg gtaatgagat ttatagtgca attaaggact
      541 tacaaagcaa agcgcctgat aaaaatatcg ttattttcac ccataatcat tgcctgacat
      601 atattgctaa aaataagcgt gatgcgacat ttaaacctga ttatctggat ggtttagtca
      661 tgcatgtgga aaaaggcaaa gtttatctgg atggggaatt cgttaaccac taaattttca
      721 atctgacagc cagtgatggc tgtcatcatt gttaaagaag acctttcaaa cgtaacgttt
      781 ttcgggtggc aacatttaaa tcataatgca ccagatcttc aggttttacc cacgcgtagt
      841 cctgaaactc ttcgtttatt ttcacttctc ggttggcaga aacgcagtca aaaatcaggt
      901 aaatcatata aatctcttcc ttgcgaccat ctgcatacgt cttggtgcga atatcatcgc
      961 tgaaggtcca cggcgtgatt tctgtcaaaa gcagctgttc tcccagttct tcgcgaattt
     1021 cgcggcgtag tgcctcttca attcgttcgc cagactccac gccgccaccc gaaagcgccc
     1081 attgaccggg gaaaacgccg cgatcgtcgg ccattttgca cagcaaataa gcaccatcat
     1141 tttgaatcaa agggcataca atagtccgtt gtcgcacagg ggtattcctt ataaataaaa
     1201 agaaaccatg agaatacggt aactcagaaa tatgtgaaag tgacagtaat ctcttttgcg
     1261 atgataactg cacttattat tatttttgtg tgtatcggta aagtgtgttt attttttcgg
     1321 cgtttaatta tttaagaagt atttagttac aataagtgac tagatccctt ttttcgctca
     1381 ttgaatcctc tgtgccaggc agtacaatcc cgcagctttg ggaattttga ttaattgctt
     1441 gtgggaaagg ggataatgaa aaaaattgcg ctagcaggtc tggccggaat gctgttggtt
     1501 tctgcatcgg tcaatgcaat gagcatcagc ggtcaggcgg gtaaagaata caccaatatt
     1561 ggtgtcggtt ttgggactga atcgacgggc ctggcgttaa gcggtaactg gacacataac
     1621 gacgacgacg gtgacgtcgc tggcgtgggg ctggggttga atctgcctct cgggccgtta
     1681 atggcgaccg ttggcggaaa aggcgtttac accaacccga attacggcga tgaaggttat
     1741 gccgcagcgg taggaggtgg tttgcagtgg aaaattggca acagcttccg tttgtttggc
     1801 gagtattact actctccgga ttcactctcc agcggtatta aaagttatga ggaagcgaat
     1861 gctggcgcgc gttacaccat tatgcatcca gtcagtattg aggcgggtta tcgctacctg
     1921 aatctgtcgg gtaaagacgg taaccgcgac aacgccgtgg ctgacggccc gtatgttggg
     1981 gttaacgcca gtttctgatc ccttccggcg cggcattctg tcgcgccatt cagtttctta
     2041 ctacgcctta tctccctttg cgttatagtg attcgataac aaaagcagga gataaccatg
     2101 ttaaaagtgg aaatgttatc caccggggat gaagtgttac acgggcaaat cgttgacact
     2161 aacgctgcct ggctggccga ttttttcttt catcaggggt tgccattatc tcgccgcaat
     2221 acggtggggg ataaccttga tgacttagtc accattcttc gcgaacgtag tcagcacgcc
     2281 gatgtgctga tcgttaacgg cgggctggga ccgaccagtg atgatttaag tgcactcgcc
     2341 gctgcgacag caaaaggtga aggcctggtg ctgcatgaag cctggctcaa agagatggaa
     2401 cgctatttcc acgaacgtgg acgagtaatg gcaccgagca accgtaaaca agcggagctg
     2461 cctgccagtg ctgaatttat caataacccg gtaggcaccg cctgtggttt tgccgtgcgg
     2521 cttaatcgtt gcctgatgtt ctttactccc ggcgtaccgt cagaatttaa ggtgatggtc
     2581 gagcacgaaa tcctgccgcg cctgcgcgag cgtttttctt taccacagtc gccggtttgt
     2641 ctgcgtttaa ctacctttgg tcgttcggaa agcgatctgg cacaaagcct ggacactcta
     2701 caactgccgc cgggtgtaac aatgggctat cgctcctcaa tgccgatcat cgaactgaaa
     2761 ctcaccggac cggcaagcga gcaacaggcg atggaaaaac tgtggctgga cgttaaacgt
     2821 gttgccggac agagcgtgat tttcgaaggc accgaaggac tgcccgcgca gatcagtcgc
     2881 gaattgcaaa accgccagtt cagcctgacg ttgagcgagc aattcaccgg tggtttattg
     2941 gctttgcaac tttctcgcgc aggtgctcca ttgctggcgt gtgaagtggt tccttcacag
     3001 gaagaaaccc tggcgcaaac tgcgcactgg attacagaac ggcgggccaa ccattttgcc
     3061 gggctggcac tggctgtttc gggtttcgag aacgagcatc tcaactttgc gctagccacg
     3121 ccagacggca ctttcgctct gcgtgtgcgt ttcagcacta cacgctacag cctggctatc
     3181 cgtcaggaag tgtgcgcaat gatggcactg aatatgctgc gccgttggtt aaacggccag
     3241 gatatcgcca gtgagcatgg ctggattgag gttgttgagt ccatgacctt atctgtctga
     3301 ttccgccact ctgtgccggt ttactgcacc ggcacattcc ccctccggca aaacttgtcc
     3361 aacctgttgt actcaataag aaaaaatccg tgcactgatt cacaaatttg tgcgtaattt
     3421 ctccagtccg ctggacgagg agcgggattt gatgcgcttc acaattttac tccccctgag
     3481 caacgagact gattccagta tctaagactg gagtccagta tgttggaatc aagcaaagtt
     3541 cccgccctga cacgggctat tgatattctc aatttgattg cccgcattgg cccctgtagt
     3601 gctgcgacga tcattgacac attgggaatc cctaaaagca cggcctattt gctgcttaat
     3661 gaactcaggc gtcagcgttt tcttagcctt gatcaccagg aaaatttttg tttgtggacc
     3721 aggctggtgg agctttctgg tcatgcgtta agcaagatgg atctccggga actggctcga
     3781 ccgcgtttga cgcaactgat ggataccacc gggctgttat gtcacctggg gattattgat
     3841 aacggcagcg cttattacat tttaaaagtg gagtcatcag ccactatcag cgtgcgttcc
     3901 catgaaggaa aaagcctttc gctttatcgc tccggaatag gtaaatgcct gcttgcctgg
     3961 caacctgcag cggtacagca aagtattatc gaagggctgg tctgggagca ggcgacaccc
     4021 acaacgatta ctcatccaca gcaacttcac gaagaactgg cgcgtattcg ccgtcagggc
     4081 tggagctatg acaacggcga agattacccc gatgtccgtt gtgtcgctgc gccggtgttt
     4141 aacgccaata acgagcttac cgctgcgatc tcggtggtcg gtacccgctt acaaatcaat
     4201 gaagaatacc gtgattatct ggcgggcaag gccattgcct gcgccaggga tatttcacgt
     4261 ctgttgggat ggaaaagtcc ctttgactta caggcctcat aacaatggag aacatcatga
     4321 ccctaccaaa aatcaaacag gttcgcgcct ggtttactgg cggtgcgaca gcagaaaaag
     4381 gtgctggcgg cggtgattat cacgaccagg gggcgaatca ctggattgac gatcatattg
     4441 ccaccccgat gagtaaatac cgcgattacg agcagtcacg ccagtcattt ggcattaacg
     4501 ttcttggcac gttgattgtt gaagtcgaag cagaaaacgg ccagaccgga ttcgctgttt
     4561 cgacagccgg tgaaatgggc tgttttattg tcgaaaaaca tcttaaccgt ttcattgagg
     4621 gtaaatgtgt cagtgatatc aaactgatcc atgatcaaat gctcaatgcc accctgtatt
     4681 actccggctc tggtggcctg gtgatgaaca cgatttcctg tgtcgatctg gctctgtggg
     4741 atctgttcgg caaagtggtc gggcttccgg tttataaact tttaggcggc gcagttcgtg
     4801 atgagattca gttctacgcc acaggcgcgc gtccggatct ggcaaaagag atgggcttta
     4861 tcggtggcaa aatgccgacg cactgggggc cacatgatgg cgatgcgggg atccgcaaag
     4921 atgccgttat ggtcgcggat atgcgtgaaa aatgcggtga ggatttctgg ttaatgctcg
     4981 actgctggat gagtcaggac gtgaactatg ccaccaaact ggcccacgct tgcgcgccct
     5041 ataacctgaa atggatcgaa gagtgcctgc cgccacagca gtatgaaggt tatcgcgaac
     5101 tgaaacgcaa cgcgccagtc ggaatgatgg tcaccagcgg tgagcaccac ggcacactgc
     5161 aatcttttcg tacgctttca gaaaccggta tcgacattat gcagccggat gttggctggt
     5221 gcggtggttt aaccacgctg gtggaaattg ccgcaatcgc caaatccagg gggcaactgg
     5281 tggtgccgca cggttcgtct gtttactctc accatgcggt gatcaccttc accaatacgc
     5341 cattcagcga attcctgatg accagcccgg attgttcaac gatgcgtccg cagtttgacc
     5401 cgattctgct caatgagccg gttccggtga atggtcgtat tcataaatca gtgcttgata
     5461 aacccggttt tggcgtcgaa ctcaatcgtg actgcaattt gaaacgcccc tacagccact
     5521 aatcactccg ggcgttgctg tgacaacggt aacgcccaca tattctaagg agctttttat
     5581 gagcaccgct ttgcttgacg ccgtggtgaa gaaaaaccgt gtgcgtttaa ttccgtttat
     5641 gttggcgctg tatgtgctgg cgtttctcga ccgttcgaat atcggttttg ccaaacagac
     5701 ctaccagatt gataccgggt tgagtaatga agcttatgcg ctgggagcag gcattttctt
     5761 tgtggtatat gcgtttctgg gtgttccggc gaatcttttg atgcgcaaac tgggggccag
     5821 aacctggatt ggtacgacaa cactgctgtg gggatttctt tcggcagcca tggcatgggc
     5881 cgatactgaa gcgaaatttc tgatagttcg cactctgctt ggtgctgcgg aggccggatt
     5941 tttccctggt atgatttatc tcacctcgca atggtttccg cagcgtaatc gcgccagcat
     6001 tatggggctg ttctatatgg gcgcaccgct ggcgttaaca ctgggatcac cgctttccgg
     6061 cgcgctgttg gagatgcatg gatttatggg gcatcccggc tggttctgga tgtttgtgat
     6121 tgaaggattg ttggcagtcg gtgctggggt attcacattc ttttggcttg atgacacacc
     6181 ggagcaggca cgttttctga gtaaacaaga aaaaacgttg cttatcaatc aactggcaag
     6241 tgaagaacaa cagaaagtga cttctcggct gagcgatgcg ctgcgtaatg gccgagtctg
     6301 gcaactagcg attatctacc tgaccattca ggtggcggtt tacggattaa ttttcttcct
     6361 gccgacccag gttgcggcat tgctgggaac aaaagtgggc tttacagcgt cggtggtcac
     6421 cgccattccg tgggttgcgg ccttgtttgg gacctggctt attccgcgct attccgataa
     6481 aaccggcgaa cggcgtaatg tcgcagcgct gacattactg gcggcgggca ttggtattgg
     6541 tctgtccggg ctgctttctc cagtaatggc gatcgtagcg ctgtgtgttg cagctatcgg
     6601 gtttattgcc gtgcagccgg tgttctggac gatgccgaca caactgcttt ccggtacggc
     6661 gctggctgcg gggattggtt ttgtaaacct gtttggtgca gtgggcgggt ttattgcccc
     6721 gatcctgcgc gtgaaagcag aaacgttatt tgccagcgat gcggcaggat tactgacact
     6781 ggcagcggtg gcggtcatcg gttcgctgat tattttcact ctgcgtgtaa atcgtactgt
     6841 tgcgcagacc gacgtggcac atcattaaat aggttaagga acacgatgaa cgcattatta
     6901 accaatccct ttaaagaacg tttacgcaag ggcgaagtgc aaattggtct gtggttaagc
     6961 tcgactactt cttatatggc agaaattgcc gctacttccg gttatgactg gttgctgatt
     7021 gacggggagc acgcgccaaa caccattcag gatctttatc accagctaca ggcggtagcg
     7081 ccctatgcca gccaacccgt gatccgtccg gtggaaggca gtaaatcgct gattaaacaa
     7141 gttctggata ttggcgcgca aactctattg atcccgatgg tcgatactgc cgatcaggct
     7201 cgtcaggtgg cgtctgccac gcgctatcct ccctacggtg agcgtggtgt tggggccagt
     7261 gtggcacggg ctgcgcgctg gggacgcatt gagaattaca tggcgcaagt caacgattcg
     7321 ctttgtctgt tagtgcaggt ggaaagtaaa acggcactgg ataacctgga cgagatcctc
     7381 gacgtcgaag ggattgatgg cgtgtttatt ggaccagcgg atctttctgc gtcgttgggc
     7441 tacccggata acgccgggca cccggaagtg cagcgaatta ttgaaaccag cattcggcgg
     7501 atccgtgctg cgggtaaagc ggctggtttt ctggctgtgg ctcctgatat ggcgcagcaa
     7561 tgcctggcgt ggggagcgaa ctttgtcgct gttggcgttg acacgatgct ctacagcgat
     7621 gccctggatc aacgactggc gatgtttaaa tcaggcaaaa atgggccacg cataaaaggt
     7681 agttattgat atcaaaggcc catggggatc ggctgtgggc ctgtgttaat tagtggttat
     7741 tcgctgccag atcggcttcg cttagctggg tggccgcgag cacctggtca cgatcaatgc
     7801 cctgttccag catgcgcaaa gcaattttaa tggcttgttc atgcatacct tcctgccagc
     7861 caatttggtg tccttcctgc cgtagccgct cagcaatagt catcaatctc tccttttgta
     7921 gtggtgaacg ttcggaaatc tcctcaataa aacgggtgaa acgggaggta tcgccgcatt
     7981 gcagcagata attaaacagt gtttgtagct ggctgtcatt agtgaagcct ttaaccaaaa
     8041 gcgtggtgat cctgtcgacc ataccgatta aatcgcggtc gcgaatatgc ttttgaatca
     8101 gttcaagcag agctatacgc cgatgttgca tgatctcatc gtcaggtacg atggtgatat
     8161 ccaccaacgg aaaagcttcg gtgtacaact gccgggcaag ttgcggatcg tcaaactcat
     8221 ccagccagtt aagtgagtac gggtagggcg atgtttcgcc atgataaaac agcaatggca
     8281 ccaccagcgg aactctgtca tagcctttat ccaggtgacg ctgcatggcg gcagtggcat
     8341 agcgcattag ccgaaaagcc atattctttt ctgcagagct ttgatgttca atcacgcagt
     8401 agatatagcc gtcaccgtcg ctggtttcca cggaccacaa aacatccgag tagtaagcgc
     8461 gtaaactttt ttcaataaaa ctagtgggtt ccaggcgtaa ggtttgcagg ttgcaaagct
     8521 tgcgcagtgg ttctggcaaa tgtatttcga gaaaatcccg tgcggtttcg ggtgtgaaca
     8581 taaaggtttt aaataccgca tcatgcgggg aggaggttgt tgattcggtc atggttgtcc
     8641 gtcacctgat atttatgatg acgcgacatt acggttatcc caggcctttg acatctgcca
     8701 gtttcagcct ttgcgagacg ttttcagagg aattttcact gcgttgcaac gcatacattg
     8761 ggcacggaat cgtgtacgca aaaatgcccg gtaaccacat gcttaccggg catcagaaag
     8821 gagtttaagc cagcgcctgg gccagtagcg taatcggatg ttcgcagcga agacttgtgg
     8881 acatctcaat ctgccattta caggtttcgc agtcggtgac caccagatct gcgccgcttt
     8941 cttctatctg gcggaacagt ggtgcgccga tggcttgtga ggtggggtag ttctcttttt
     9001 tgaaaccgta agtacccgca ataccgcagc actgggaatc cagcaccgtt aactcaagcc
     9061 ccgggatttt acgcaacagc tccagggtgt agagcgtcca gcccattttt tccatatggc
     9121 acggagtgtg ataaaccact ttcagcggca gcggtttcag cggtaacgtt ttgccttcgt
     9181 ccagcttgcg ccacagccag cgggttgcca gttcgatatg atcgcgcaag cctttgttgt
     9241 cgacattcag cacttccggg tattcgtcgc gcagggcaaa tgtacaggtt gaggaggtgg
     9301 caatcaccgg aatgcctttt actcccacag cttcgcggat cgactctaca ttcgtaattg
     9361 cctgtttgcg tgctttatcg gtaaagccgt tggcgattag cggtacgccg cagcattttt
     9421 ctttgctgag cagttgtaca ccgataccca ttgcgttgag cactttaatt aaatctttac
     9481 ctaactgcgg atggttgtag ttaacgaagc agccgtgaaa gaaagcgacc tggtctttat
     9541 attgtgcttg ctgagccgct atgctgcgat accagcgacg gaacgtgccg aaggagtatt
     9601 tcggtagcgt gcgacgatga tcgattttta acgccgcatc aagcagctgc cgcaccggtt
     9661 tcagcgaggt agcggtgttg acgattggtg cgaacggcgt ggaaacgcta cccatcaggt
     9721 cggtatgact caacacaaaa ttacgcagcg acgggcgcgt ggtgtcatat tttgcccgcg
     9781 cgcgctggat aatatcgcca atcttcacat cggacgggca ggcgacttca caacgtttgc
     9841 agttgatgca atatttcagc gcctcgtcat acagtgcgcc atctttcaaa cgcagacgct
     9901 cgccatccgg cccggcttgt tttggccctg gataaccggg attcacccgg ctcaccgggc
     9961 aggcggtggt gcagacggtg cacttaatgc agttttcgaa gctggtgtcg ttcattgttg
    10021 gcctcctgcg cgttgggcaa tctgttgagc ggcatgtaaa gcactgacgg cacaaacacc
    10081 gccgccgcat ccctgggcga tgggatcaaa tccgcccagc accgaaccga tggcaaacag
    10141 gttctcaatg gtttgccctg cctgcgacgg gcgtagcgtc tcatcagtgg ttacaccgaa
    10201 ctgctgccac ggttgcggcg caaaaaaatc gcccttatac cattcacccc gcgtggcggt
    10261 ttgtagcaca tcaaggccga gaatcggctc gcgaatgccg ttacgttccg ctaccagtcc
    10321 gccactaaag aaactgccgc tggcgagaac cgcgaaacgt ggacgtagcg gaatatcggc
    10381 gtgattgcgg gtccagattt cattcactac gccattttta caggtcactt ttttcacttc
    10441 atcgcccggc atccacacgc caccctggcg cacaaactgg cgctgtaact ggttttgcag
    10501 acgaatgccc agcacggaag gcggcagcgt tggcaaaagc atcagtgaac aaggtagttt
    10561 ttcattcaac caacgccaca gtttgtcatc ggccaggccg aagcaggcgg gcatcaggat
    10621 catttcgcag gtattggcga caggaataag cgcatcaagt aacagcggcc agttttcttc
    10681 attatcaagg aaacgggcga tattcaccgc gcgaaattcg gtggcgttat tgcgcaggac
    10741 atccagttcc ggcagctcta tttctgcggt ttcaaccgaa agatccagtt cgcgcaacga
    10801 agccgccgca agatgcgcct ggaaatccat cagcccgctt atccccacta cacatatctt
    10861 cttcgcgggt agcggccaga cgggaacttc tggcgaactt aaccaggttg cacgcaaagt
    10921 gccgagcggg gtaacgcgct gatgggccag ttccacgctg ccctgcaatt gcgcaccgct
    10981 ttcagcgatc aatgcctgcg cctggcaagc gagatcgagc acgcgttgtg gttcgagaag
    11041 actgtaagga tgggctggtg cctgctgacg caaagattcc agtccactgt gaatgtctgt
    11101 caccggttga ccatcaggca gatggctcag caaatccagc gaaccggatg agaaatgcag
    11161 cgcgctttga ccacgcgtga caatggcaca gcgcaggccg tgtttttgca gttgcaggcc
    11221 acagagtaat ccggcgaggc cgccgcccat aatgacagta tcaaagcgca tctttctgct
    11281 ccttctccag accacacagt ccctgataaa cccagcgggt aaattcgctt tcgcgcagtg
    11341 catctcccca ggcgatgggt tgcacgcctt tccagcgttc gttaaggaag gtggaaagtt
    11401 gctcgataga ttgcgcggac gtcgtgacgt taaaacgttg cagcagtccg gcagcgcggc
    11461 aggcgcagag ttcgccctgg caggtaccca tccccacacg ggtacgacgg cgtaaatcca
    11521 gcaggctatt aacgtttaaa ttttctaccg cgtactgcac ttcacccgct gttaccgctt
    11581 cgcactcaca taccaggcta cggtgcagac gaccttcgct cagccaggca ggcgtgcgat
    11641 cgccatgacg ataaaccgca gaaccgcgca gcggggcagg cagggagatg actttacgca
    11701 aggtaacttc agcgggttcc tgtgaaccag gaagtgccag atcggcggtc gtacaggggc
    11761 gcgtgttgcc cagtttgcgg cacacggcgt cggtagccca ttcagccatc agccgatagg
    11821 tcatcagttt gccaccggtg atggtgataa atccgtccag accatcgcgt tcagcatggt
    11881 cgagcagcac gatgccacgg ctgacgttac gtccgctcgg gtcgtcatcg ctggcaacca
    11941 gcgggcgcac gccagaatag gcccgcaaaa tgcgcgtttt cgccatcacg ggggccagtt
    12001 tttccccttc acgcagcaga atatcaacct cttctgccgt cactcgatta tcgtcaatct
    12061 cgttgtaatc aatacgtaaa gaggtggtgc caatcagcga aatggtatcg ccaggcacca
    12121 gaatatcggc gtcggaaggt ttacggcagc ggttgatcac atgctggtta atgcggtgat
    12181 ccatgatcag cagcgatcct ttcgccggga acatgcgaat gcgcagatcg gcatattcgg
    12241 caatgtgttg cccccagatc ccggcggcat taaccacgac aggcgcatga agggcctgag
    12301 tttcgccggt gagatggtta cgtacacgaa caccgcacac cgtcgcgcct tcacgaatca
    12361 gtcccgtgac ttcatgagcg gtaaggataa cggcaccgtg ttctttggca tccagcatgt
    12421 ttgctgcggt cagacgaaat ggatcaacgg tgccatccgg aactttcacc gcgccaatca
    12481 atgccgggtt aacggcaggt tcgataatgc gcgcttgctg cgggtctata gcttctgcgc
    12541 tgatccctgc ttcttcgcag gcgcgaataa aagtggcctg gaaggagagg tcatcttccg
    12601 gcagggtaat aaacaggccg ttggttggtt caacgcagtg acgtgcaatg cgtttcagga
    12661 tctggttttc actaatgcat tcgcgggccg attccgcatc ggttaccgca tagcgcgcac
    12721 cgctgtgtag caggccgtgg ttacgcccgg tggcaccggt tgcgatgtcg tggcgctcaa
    12781 ccaaaatcac gcgcagcccg cgcagggcac agtcgcgggc aatcccggct cccgttgcgc
    12841 cgccgccaat gataatcacg tcacttgatt gcgagtcgcg agttttcatt gttatccctc
    12901 tgaagttcgt tttttaccat ttagccatag taaaaacatg aattgtttga tttcgcgcat
    12961 attcgctcat gattcgaaag tgaaacgtga tttcatgcgt cattttgaac attttgtaaa
    13021 tcttatttaa taatgtgtgc ggcaattcac atttaattta tgaatgtttt cttaacatcg
    13081 cggcaactca agaaacggca ggttctctca ctgaatcagg ctgttaatca taaataagac
    13141 cacgggccac ggaggctatc aatgttgagt atttttaaac cagcgccaca caaagcgcgc
    13201 ttacctgccg cggagatcga tccgacttat cgtcgattgc gctggcaaat tttcctgggg
    13261 atattctttg gctatgcggc ttactatttg gttcgtaaga actttgcgct tgctatgcct
    13321 tatctggttg agcagggatt ctcacgcggt gatttaggtt ttgccctttc ggggatctcg
    13381 attgcttatg gattttcgaa attcatcatg ggttcggtat cggatcgctc gaatccgcgc
    13441 gttttcctgc ccgcaggttt gattctggcg gcggcagtga tgttgtttat gggctttgtg
    13501 ccatgggcga cgtcgagcat tgcggtgatg tttgtactgt tgttcctctg cggttggttc
    13561 caggggatgg ggtggccgcc gtgtggtcgt actatggtgc actggtggtc gcagaaagaa
    13621 cgtggcggca ttgtgtcagt gtggaactgt gcgcacaacg tcggtggtgg tattccgccg
    13681 ctgctgttcc tgctggggat ggcctggttc aatgactggc atgcggcgct ctatatgcct
    13741 gctttctgcg ccattctggt ggcattattc gcctttgcga tgatgcgcga taccccgcaa
    13801 tcctgtggct tgccgccgat cgaagagtac aaaaatgatt atccggacga ctataacgaa
    13861 aaagcggaac aggagctgac ggcgaagcaa atcttcatgc agtacgtact gccgaacaaa
    13921 ctgctgtggt atatcgccat cgccaacgtg ttcgtttatc tgctgcgtta cggcatcctc
    13981 gactggtcac cgacttatct gaaagaggtt aagcatttcg cgctggataa atcctcctgg
    14041 gcctacttcc tttatgaata tgcaggtatt ccgggcactc tgctgtgcgg ctggatgtcg
    14101 gataaagtct tccgtggcaa ccgtggggca accggcgttt tctttatgac actggtgacc
    14161 atcgcgacta tcgtttactg gatgaacccg gcaggtaacc caaccgtcga tatgatttgt
    14221 atgattgtta tcggcttcct gatctacggt cctgtgatgc tgatcggtct gcatgcgctg
    14281 gaactggcac cgaaaaaagc ggcaggtacg gcagcgggct ttaccgggct gtttggttac
    14341 ctgggcggtt cggtggcggc gagcgcgatt gttggctaca ccgtggactt cttcggctgg
    14401 gatggcggct ttatggtaat gattggcggc agcattctgg cggttatctt gttgattgtt
    14461 gtgatgattg gcgaaaaacg tcgccatgaa caattactgc aaaaacgcaa cggaggctaa
    14521 tggcatgaaa ttgaagctga aaaaccttag catggcgatc atgatgagca ctatagtcat
    14581 gggaagcagt gcaatggcgg cggacagcaa cgaaaaaata gtcatcgccc atcgcggtgc
    14641 cagtggatat ttgccggagc atacgctgcc agcaaaagcg atggcgtatg cgcagggagc
    14701 ggattatctg gaacaggatt tggtgatgac caaagacgac catctggttg ttctgcatga
    14761 ccattatctc gatcgtgtta ctgatgttgc cgatcgtttc ccggatcggg cgcgcaaaga
    14821 cggtcgttac tacgcgatag atttcacgct ggatgaaatt aagtcgctga aatttaccga
    14881 aggtttcgat attgaaaacg gtaaaaaagt acagacttat ccggggcgtt tcccaatggg
    14941 taagtccgac ttccgggggc acacctttga agaagagatt gaatttgttc aggggttaaa
    15001 tcactctacc gggaaaaata tcggtatcta tccagaaatc aaagcgccgt ggttccatca
    15061 tcaggaaggg aaggatattg cggcaaaaac gctggaagtg ctgaagaaat atggttacac
    15121 cggtaaggac gataaagttt atttgcaatg ttttgatgct gatgagctga agcgtattaa
    15181 gaatgagctg gaacctaaaa tgggcatgga tctcaatctg gtacagctga ttgcctatac
    15241 cgactggaat gaaacgcagc agaaacagcc ggacggaagc tgggttaatt acagctatga
    15301 ctggatgttt aagccaggtg ccatgaagca agtcgcggaa tatgccgacg gtatcgggcc
    15361 ggattaccat atgttgattg aggaaacatc gcagccgggt aatatcaaac tcactggcat
    15421 ggtgcaagat gctcagcaga ataaactggt agtgcatcct tataccgtgc ggtcggataa
    15481 actgcctgaa tacactactg atgtgaatca gttatatgat gctctgtata acaaagcggg
    15541 tgtaaatggg ctgtttactg atttccctga taaggcagta aaattcctta ataaagagta
    15601 atgctactgc aggatggcgt tcatgcgcca tcctgtctca tatcaaattt ttccaatcgc
    15661 cactaattgt tccatgcaca tttgtagtaa aaacagtgga ctgccttcta ccggcttttc
    15721 tttagccaac aacgaaatct gtcgatagaa ggttggttcg tgaggaacgg aacacaattc
    15781 acctgcaatg gttttcagcg ttaactctgg taataaggcg atgcctaatc cctgtcgaat
    15841 aaaattcatc gctgtatcca cctgattaaa ttcacatttt atctgctggg cgcactatta
    15901 gtggtcgtgg ctggtggaat ctatgcgctg caaatgaagg gcaaaagcgg tgtca
//
LOCUS       DAZRUF010000083        15885 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZRUF010000083 DAZRUF010000000
VERSION     DAZRUF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15885
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.083"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            92..1132
                     /locus_tag="ACHXOL_002107"
     CDS             92..1132
                     /locus_tag="ACHXOL_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR6809083.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHXOL_002108"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHXOL_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR6809084.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHXOL_002109"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHXOL_002109"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR6809085.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="ACHXOL_002110"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="ACHXOL_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR6809086.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHXOL_002111"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHXOL_002111"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR6809087.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="ACHXOL_002112"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="ACHXOL_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR6809088.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHXOL_002113"
     CDS             complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHXOL_002113"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR6809089.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHXOL_002114"
     CDS             complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHXOL_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR6809090.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="ACHXOL_002115"
     CDS             complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="ACHXOL_002115"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR6809091.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11821..12657)
                     /gene="kduI"
                     /locus_tag="ACHXOL_002116"
     CDS             complement(11821..12657)
                     /gene="kduI"
                     /locus_tag="ACHXOL_002116"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR6809092.1"
                     /translation="MDVRQGIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12944..14125)
                     /locus_tag="ACHXOL_002117"
     CDS             complement(12944..14125)
                     /locus_tag="ACHXOL_002117"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR6809093.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14380..15609
                     /locus_tag="ACHXOL_002118"
     CDS             14380..15609
                     /locus_tag="ACHXOL_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR6809094.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         4108 a         4264 c         3725 g         3788 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
     8821 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
     8881 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
     8941 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
     9001 tcgacgctat gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct
     9061 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
     9121 attgttttca ttcctctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg
     9181 agacaaacgc ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg gccggatgta
     9241 cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag
     9301 tttgcgttcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtgat
     9361 gcccacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag caccaatgct
     9421 gtcgagcagc gtcaggaagg tcgcgccgat aatcatattc gacacccagt tcgtggtggt
     9481 cgaacaggta ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca
     9541 caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga
     9601 gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag
     9661 caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggtt tacgccctgc
     9721 tttatctacc gtaaacaccg caataaaggt ggcgaacata aaggtcagcc ctacgaccag
     9781 agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc
     9841 gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag
     9901 aaacacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa
     9961 cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag
    10021 catacgcaac acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct
    10081 atttggcagg aagaccacca gaataatcag caggactgct ggtaaagcaa gaacccccaa
    10141 cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat
    10201 gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc
    10261 catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac
    10321 cacccgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag
    10381 tacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca
    10441 accattaaac agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg
    10501 caaacgactg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc
    10561 gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat
    10621 acgccgcgta tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat
    10681 tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaac tcacacaggt
    10741 gtcaggtcgg aaacagcata aatatggatt aaattgctgc gacatgtcgt tatgtgatgg
    10801 atattccaat tttcaaatta agttgaatta ttgagattat tattaaccac ctaattttac
    10861 agcagataaa attcataaag ttcattaatt gataattgat atggattatt tcataaccat
    10921 gatatgaatt atgatgacct acaggtataa aaaaccctgc catgaggcag ggtcataaaa
    10981 gtaagaggaa tgaattaacg agccagccaa ccgccatcta cggcaatggt ataaccattc
    11041 acataatctg aagcgctgga ggcaaggaac actatcggcc ccatcaggtc actcggcagt
    11101 ccccaacgac cagctggaat gcggtcgaga atttccgcgc tacgttgttc atctgcccgt
    11161 agttgttgag tattgttggt cgccatgtaa cccggagcta tcgcattaac attaatgttg
    11221 tgtttagccc attcgttcgc catcaatcgc gtcacgccca tcacgccgct ttttgatgcg
    11281 gtataagaag gcacacggat cccgccctgg aaggagagca ttgacgtgat attgataatc
    11341 ttgccgccat tgcgttgcgc gataaagtgt ttcgccgctg cctgagacat gaagaatacg
    11401 ctcttgatat tcaggttcat gacatcgtcc cagtcttttt cgctgaactc gagagcatct
    11461 tcgcggcgaa tcaatccggc gttattcacc aggatatcaa tatgaccaaa ctcagctacc
    11521 gcgcgatcca gcagtgctgg aatgccatca atctttcgca gatcggcggt caggcttaaa
    11581 aaacgacgcc ccagcgccgt gacctgcttg atggtttcag tcggttcaac gatgttaatg
    11641 ccaacaatgt cacagcccgc ttgcgccagc cccaacgcca tcccctggcc cagtccagta
    11701 tcacaaccag tgacgaccgc aactttacct tcgagagaaa atgcacttaa aatcataaca
    11761 ataccttatt cgtttatgcc gacaactagc gcaaatcttt aacggccact atgccgacaa
    11821 ctagcgcaaa tctttaacgg ccacatggtc catatcatca aagacctggt tttcaccaac
    11881 catgccccag ataaaggtat aagctttggt tccgacgccg gaatggatcg accagctggg
    11941 ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg gctgccccat
    12001 catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt ccatccggcg
    12061 ctcgtgagtg tgacacggca tggtgttcca caagttacct ggagccagct ccgtcagccc
    12121 catactcaat tggcaggttt ccagcacatc cgggacaaaa tatttgttaa ttgtgcgacg
    12181 gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccggtg tgactttttt
    12241 ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aactttgccg gagtggcggt
    12301 atcaatactg gcaaagacaa cttcttttgc acctttacca acatacaggg cgtcgcggtg
    12361 accgatttca tagcattggc catcgacagt aatcgtaccg gcaccgccaa tattgataac
    12421 acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa cttccccgcc
    12481 aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt caatgtggct
    12541 gtaaaccatg gtgtactcat cggcgacaaa taccttttca accaaaaatt cattgcgcag
    12601 cccttgggta tccagcgttt ttgcgtgcgc actgtggatg ccctgtctta cgtccacatc
    12661 aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt
    12721 tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat aaaacaatgt
    12781 ttcattttta agttaaggat taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa
    12841 tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat tattcaacag
    12901 attaatgacg gcagatattt atcggtaagg aggaaatgca gagctactcg tcacgttcaa
    12961 tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct ttgcgggcat
    13021 tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa gcgcctatcg
    13081 ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc cactcaagca
    13141 tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata agatcgacat
    13201 cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc ggcgcaattc
    13261 ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta
    13321 aattcaacgc tcgtgctttg gcttcgctca tcatcattac cgcagctgcg ccatcgttta
    13381 tggatgatgc attccccgcg gtcacagaac cgagactatc aaatgaagga tttaaacggg
    13441 ctaaaccttc tgcactggcg tcagtgcgtg gctgttcatc ggtatcaaca accaacgtct
    13501 gaccgttact ttggatcatt accgggacga tctcatcttt aaatcgtccg gcgtcaatcg
    13561 ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc ctgcaactga cggctgatgc
    13621 catattcgcg agccagattt tcggcggtga caccaatatg ataatcattg aaggcatccc
    13681 acaacccatc atgcaccaga ctgtcaacca actggctatt gccaagctgt gcaccggtgc
    13741 ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg gcgatgacaa
    13801 tatcagcctc gccacactgt atcgcctgag tagccagatg cagtgcttta agtccggaac
    13861 cgcaaacgtc attaatcgtg attgcagaaa cgctattagg aagaccacct ttaatagccg
    13921 attgccttgc cggattctgc cctgcccctg cagtcaacac ctgaccaaga attacttcat
    13981 ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc agactaccaa
    14041 gttccacggc ggaatgaccc gctaacgcac cacgaaagca gccgataggt gtccgtaacg
    14101 ccccgacaat cacaacgtct ttcatcacaa cctcgcacca attaacagtg caatagtaaa
    14161 tgattgttaa ttacagttat cttaattgtt taaaaaaagt gatttttatc acaaaggaaa
    14221 tatgcctgag cagtagtcag agtcataact ggcacgtaag gtttgcaacc actaacccac
    14281 caatagatgg gtagataagg cgttaatctc ccatacttaa cctggtttat ggtaaattgc
    14341 cctccatttt gtttaatttg tagatgatac gttcagataa tgtctaatat ttggtctaaa
    14401 gaagaaactc tgtggagttt cgcgctctac ggcacagccg ttggtgcagg cacgctcttc
    14461 cttcctattc agttaggttc ggcaggggct gtggtcctgt ttattactgc tctggtcgcc
    14521 tggcctttaa catattggcc acataaagcc ttatgccagt tcatcctctc atcaaaaaca
    14581 tcagcatgtg aagggataac gggcgcggta acacactact atggcaagaa gattggtaat
    14641 ctgattacca cgctgtactt catcgccttt tttgtcgtcg tgttgatata tgcagtggca
    14701 attaccaact cacttacgga acagctggca aagcatatgg ttattgatct tcgcatccgt
    14761 atgttggtga gtctgggtgt tgtattaatt ctgaatctca tttttctgat gggacgtcat
    14821 gccactattc gggtaatggg atttttggta ttcccattga ttgcctattt cttatttctt
    14881 tccatttacc ttgtcggtag ttggcaacct gatctattaa cgacccaggt agagttcaat
    14941 cagaataccc ttcaccagat atggatatcg attcccgtga tggttttcgc ctttagccat
    15001 acgcccatta tttctacgtt tgccatagac agacgtgaaa aatatggcga acacgctatg
    15061 gataaatgca aaaaaattat gaaagtcgct tatctcatca tctgcataag tgtactgttc
    15121 tttgtcttta gctgcctgct ttctattcca ccttcgtata ttgaagcggc taaagaagaa
    15181 ggggtcacca ttttatcggc gctttctatg ctgccgaacg ccccagcatg gttgtcaatt
    15241 tccgggatta ttgtcgcagt agttgcgatg tcgaaatcat tcctgggtac gtactttggc
    15301 gttattgaag gtgccacgga ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa
    15361 agtcgtgcat ttaaccgcgc actatcaatt atgttggtat cgctgattac cttcattgtt
    15421 tgttgcatta acccgaacgc gatttcgatg atttatgcga tcagcggccc gctcattgcc
    15481 atgatacttt tcatcatgcc tacgttgtca acgtatctga tcccggcgct taaaccctgg
    15541 cgctccatcg gaaatctgat tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc
    15601 tttagctaat atccgttgtg gatggttcaa aattgctaca attttgaacc attccatttt
    15661 tcataatatt catttgttat tattccgccc tatcacttct ctcgtcatat cctagaatta
    15721 tcagttagtt aaacacacaa agagatctga acatcttaaa taagactatt taagatgcat
    15781 aacttagatt cgcaagatat cttctggtca ttcaaaaaca atttgcgata tagatattct
    15841 cataccatga gatagttatc tggaccacgt tcgaccatgc aagat
//
LOCUS       DAZRUF010000084        15636 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZRUF010000084 DAZRUF010000000
VERSION     DAZRUF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15636
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.084"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            29..910
                     /gene="yieE"
                     /locus_tag="ACHXOL_002119"
     CDS             29..910
                     /gene="yieE"
                     /locus_tag="ACHXOL_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR6809095.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1115..2584
                     /gene="lysP"
                     /locus_tag="ACHXOL_002120"
     CDS             1115..2584
                     /gene="lysP"
                     /locus_tag="ACHXOL_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR6809096.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2876..4867
                     /gene="cirA"
                     /locus_tag="ACHXOL_002121"
     CDS             2876..4867
                     /gene="cirA"
                     /locus_tag="ACHXOL_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR6809097.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4899..5735)
                     /gene="yeiG"
                     /locus_tag="ACHXOL_002122"
     CDS             complement(4899..5735)
                     /gene="yeiG"
                     /locus_tag="ACHXOL_002122"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR6809098.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5993..6661
                     /gene="folE"
                     /locus_tag="ACHXOL_002123"
     CDS             5993..6661
                     /gene="folE"
                     /locus_tag="ACHXOL_002123"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR6809099.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6678..7835
                     /gene="yeiB"
                     /locus_tag="ACHXOL_002124"
     CDS             6678..7835
                     /gene="yeiB"
                     /locus_tag="ACHXOL_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR6809100.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            8019..9017
                     /gene="galS"
                     /locus_tag="ACHXOL_002125"
     CDS             8019..9017
                     /gene="galS"
                     /locus_tag="ACHXOL_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR6809101.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            9297..10295
                     /gene="mglB"
                     /locus_tag="ACHXOL_002126"
     CDS             9297..10295
                     /gene="mglB"
                     /locus_tag="ACHXOL_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR6809102.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10356..11876
                     /gene="mglA"
                     /locus_tag="ACHXOL_002127"
     CDS             10356..11876
                     /gene="mglA"
                     /locus_tag="ACHXOL_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR6809103.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKFRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11892..12902
                     /gene="mglC"
                     /locus_tag="ACHXOL_002128"
     CDS             11892..12902
                     /gene="mglC"
                     /locus_tag="ACHXOL_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR6809104.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(13029..14264)
                     /gene="preA"
                     /locus_tag="ACHXOL_002129"
     CDS             complement(13029..14264)
                     /gene="preA"
                     /locus_tag="ACHXOL_002129"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR6809105.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14258..15496)
                     /gene="preT"
                     /locus_tag="ACHXOL_002130"
                     /pseudo
     CDS             complement(14258..15496)
                     /gene="preT"
                     /locus_tag="ACHXOL_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
BASE COUNT         3725 a         3835 c         4114 g         3962 t
ORIGIN      
        1 aattaatctt tataagtggt aagcgactat gcacatcacc ctccggcagt tggaagtttt
       61 tgcagaagta ttgaaaagtg gatcaaccac ccaggcgtcg gtgatgctgg cgttgtcgca
      121 atcagcagtg agcgcagcct tgaccgacct ggaagggcag cttggcgtgc aactgtttga
      181 tcgcgtgggg aaaagactgg ttgttaatga acacgggcgg ctgctctatc cgcgtgcgtt
      241 ggcattgctt gaacaggcgg ttgaaatcga acaactgttt cgcgaagaca acggcgcgat
      301 tcgtatctat gccagtagta ccatcggtaa ctacattctg cctgcagtta tcgcccgtta
      361 tcgccatgat tatccgcagt tgccgattga acttagcgtt gggaacagcc aggacgtgat
      421 gcaagcggtg ctggatttcc gcgttgatat tggctttatt gaaggaccgt gccacagcac
      481 tgaaatcatt tctgaaccgt ggctggaaga cgagctggtg gttttcgccg cgccgacttc
      541 gccgttggct cgtggtccgg tcaccttaga acagctggcc gctgcgccgt ggatcctgcg
      601 tgaacgcggt tccggcacgc gggagattgt cgattatctg ttgctgtcac atttaccgaa
      661 gtttgagatg gcgatggaat taggtaactc cgaggcaatc aaacatgcgg tgcgtcatgg
      721 gttgggaatt agttgcctgt cgcgacgtgt gattgaagat caattgcagg caggcacatt
      781 aagtgaagtt gcggtccctc tcccgcgcct gatgcgtacg ttgtggcgta tacatcatcg
      841 gcaaaaacac ctttccaacg cgctacggcg ctttctggac tattgcgatc ccgcgaatgt
      901 accgcgttaa ggtgctgtac aagaacatgc tggtgctgtg tcgatttcgt gacgcagcgc
      961 cctcagcatg cattcgccag aaaagagatt ggctgcttta cttataatcc ctgggcgatc
     1021 atgaaggtgt cttataaccg tgtatttctg ccggaaggat tgccaatcgt ctgctacaat
     1081 cgcgcctcat ttttaagatg gatagcattt ttgtatgggt tccgaaacta aaactacaga
     1141 agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg acgatgattg ccattggcgg
     1201 ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg atttctcagg caggtccggg
     1261 cggggcattg ctctcgtata tgctgattgg cctgatggtt tacttcctga tgaccagtct
     1321 cggtgaattg gctgcatata tgccggtttc cggttcgttt gccacttacg gtcagaacta
     1381 tgttgaagaa ggctttggct tcgcgctggg ctggaactac tggtacaact gggcggtgac
     1441 tattgccgtt gacttggttg cagcacagct ggtcatgagc tggtggttcc cggatacacc
     1501 gggctggatc tggagtgcgt tgttcctcgg cgttatcttc ctgctgaact acatctcagt
     1561 tcgtggcttt ggtgaagcgg aatactggtt ttcactgatc aaagtcacga cagttattgt
     1621 ctttatcatc gttggcgtgc tgatgattat cggtatcttc aaaggcgcgc agcctgcggg
     1681 ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt ggttttgcgg cgatgatcgg
     1741 cgtagctatg attgtcggct tctctttcca gggaaccgag ctgatcggta ttgctgcagg
     1801 cgagtccgaa gatccggcga aaaacattcc acgcgcggta cgtcaggtat tctggcgaat
     1861 cctgttgttc tatgtgttcg cgatcctgat tatcagcctg attattccgt ataccgatcc
     1921 gagcctgctg cgtaacgatg ttaaagacat cagcgtcagt ccgttcacct tggtgttcca
     1981 gcacgcgggt ctgctctctg cggcggcggt gatgaacgca gttattctga cggcggtgct
     2041 gtcagcaggt aactccggta tgtatgcgtc tactcgtatg ctgtacaccc tggcgtgtga
     2101 cggtaaagcg ccgcgcattt tcgctaaatt gtcgcgtggt ggcgtgccgc gtaatgcgct
     2161 gtatgcgacg acggtgattg ccggtttgtg cttcctgacc tccatgtttg gcaaccagac
     2221 ggtatacctg tggctgctga acacctccgg gatgacgggt tttatcgcct ggctggggat
     2281 tgccattagc cactatcgct tccgtcgcgg ttacgtattg cagggacacg acattaacga
     2341 tctgccgtac cgttcaggtt tcttcccact ggggccgatc ttcgcattca ttctgtgtct
     2401 gattatcact ttgggccaga actacgaagc gttcctgaaa gataccattg actggggcgg
     2461 cgtagcggca acgtatattg gtatcccgct gttcctgatt atttggttcg gctacaagct
     2521 gattaaagga actcacttcg tacgctacag cgaaatgaag ttcccgcaga acgataagaa
     2581 ataagtttcc tcccttcctt gctaagccct ctcaaccgag agggcttttt caattccatt
     2641 tccctgacaa atcatgcgga tataaaattt aacatttgga ttgataactg ttatcgtttg
     2701 cattatcgtt acgctacaat caaaaaaggc tgacaaatca gaggctggtc cggctttctg
     2761 ggatgatcac ctgcatacaa ataagtccac cgcgatgctg ccgtacgcaa ggggacgtaa
     2821 agaagatgtg agcgataccc attttatttt cgtagttacc tcatggagat atggaatgtt
     2881 taggttgaac cctttcgtac gggtcgggct gtgtttgtcc gctatttctt gtgtatggcc
     2941 tgtgttagcg gtcgatgacg atggcgaaac gatggttgtc actgcatctt ccgttgaaca
     3001 aaaccttaaa gatgcacccg ccagtatcag cgtcattacc caggaagatc tgcagcgaaa
     3061 accggtacag aatctgaagg atgtcctcaa agaagtgcct ggcgtacaac tgacgaacga
     3121 aggggataac cgtaagggcg ttagtattcg tggtctggac agcagctata ccctgattct
     3181 cgtcgacggt aaacgcgtga actcccgcaa tgccgtcttc cgccacaatg atttcgatct
     3241 gaactggatc ccggtcgatt ccatcgaacg tattgaagtg gtccgtggcc cgatgtcgtc
     3301 gctgtacggt tccgatgcgc tcggcggtgt agtgaatatc atcaccaaaa aaatcggtca
     3361 gaaatggtcg ggtaccgtta ccgtcgatac caccattcag gaacatcgcg atcgcggtga
     3421 tacctataac ggtcagttct ttaccagtgg accattaatt gatggtgtgc tgggaatgaa
     3481 agcttacggc agcctggcaa aacgtgaaaa ggatgacccg caaaactcaa cgaccaccga
     3541 taccggagaa acgccgcgta ttgaaggatt ctccagccgc gacggcaatg tcgaatttgc
     3601 ctggacaccg aatcaaaatc acgattttac tgccggatac ggtttcgacc gtcaggatcg
     3661 tgattccgac tcgctggaca aaaaccgcct ggaacgccag aactactccg tcagccataa
     3721 tgggcgttgg gattacggca ccagcgaact gaaatactac ggtgagaaag tcgagaacaa
     3781 aaaccctggc aacagcagcc cgataacttc cgaaagcaat acggtcgacg gcaaatacac
     3841 gttgccgctg acggcgatta atcagtttct cacggttggc ggtgaatggc gtcacgacaa
     3901 acttagcgat gcggtgaacc tgaccggggg aaccagctcc aaaacgtctg ccagccagta
     3961 cgcgctgttt gtggaagatg aatggcggat cttcgagccg ctggcgctga cgaccggcgt
     4021 gcgtatggac gatcacgaaa cctacggtga acactggagt ccgcgtgcct acctggttta
     4081 taacgccacc gacaccgtaa cggtgaaagg gggctgggcg acggcattta aagcaccttc
     4141 tctgttgcaa cttagccctg actggacgag caattcctgc cgtggcgcat gtaagattgt
     4201 gggtagcccg gatctgaaac cagaaaccag cgaaagttgg gagctggggc tttactacat
     4261 gggtgaagaa ggctggctgg aaggggttga atccagcgtt accgttttcc gtaacgatgt
     4321 gaaagatcgt atcagcatca gccgtacgtc tgacgtcaac gctgcaccgg gctaccaaaa
     4381 ctttgttggt tttgagacgg gcgctaacgg acggcgcata ccggtattta gctactacaa
     4441 cgttaacaaa gctcgtattc agggcgtgga aaccgaactg aaaattccgt tcaacgatga
     4501 atggaaactg tcgatcaact acacctacaa cgatggtcgt gatgtcagca acggcgaaaa
     4561 caaaccgcta tccgatctgc cgttccatac tgctaacggt acgctggact ggaaaccgct
     4621 ggcgctggaa gactggtcat tctatgtttc tggtcactat accgggcaga aacgcgccga
     4681 cagcgcgacg gctaaaacac cgggcggtta taccatctgg aataccggcg cggcctggca
     4741 ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac cttggcgaca aggatctcag
     4801 tcgtgacgac tacagctata acgaagacgg acgtcgttac tttatggcag tggattatcg
     4861 cttctgatga gaagatgccc ggcgaaccgg gcggactttc acttcagtaa atactgcgca
     4921 tggaagcgca ggtgatcctc tataaaagag gcgatgaagt agtaactgtg atcatatccc
     4981 ggctgaatac gcagcgtcat cggccacgct ttctggcgcg cggcttctgc caataccgca
     5041 ggttgcaact ggtcggcaag aaactgatca ttatcgccct gatcgataag cgtcgggatc
     5101 gcatcctgcg cgttactggc atacatcagc gcgcaactat cccattccag ccatgcattt
     5161 ttgtcctcac ctaaataggt gctaaacgct ttgattcccc acgggacgct gcacggattc
     5221 acaattggcg caaaggccga aacgctggtg tatttacccg gatttttcag cgccataatc
     5281 agcgcaccgt gaccacccat tgagtgaccg ctaatggcgc agcggtcgct gacattaaat
     5341 tgcgactgaa ccagcgccgg taattcatcg cgcagataat catacatccg gtaatgcgtc
     5401 gcccacggcg gttgcgtggc attaagataa aagcctgcgc cctggcctaa atcgtagcca
     5461 tcgtcgttgg caaccttttc gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc
     5521 agttctgccg ccacgcgctg ggcacccgcc ttggtggtga agttctcgtc attgcaggtt
     5581 aatccggaaa gccagtacag cactggtggc ggggtgtgat cacgaggggg tgggagaaag
     5641 atactgaacg tcatcgggca gtttaaggta ctggagtcgt gtcgccagcg ttgctgccag
     5701 ccttcaaaac agcggtgctc ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg
     5761 caatttcttc atcataccgt ttattcaatg tgctgtgagt aactttcact tccgtatttg
     5821 cataacgatg ttttaacatc tgctgatgaa agtcagcggc aattacaata attatcgctg
     5881 tgaatactgg attatgtgcg ccgcctcacg cacaataatc aggctgtaaa tcagcttaat
     5941 aactttgccc ccacgcaggg cggaggcgtc acacctgcag gagaaatcat aaatgccatc
     6001 actcagtaaa gaagcggccc tggttcatga agcgttagtt gcgcgaggac tggaaacacc
     6061 gctgcgcccg cccgtgcatg aaatggataa cgaaacgcgc aaaagcctta ttgctggtca
     6121 tatgaccgaa atcatgcagc tgctgaatct cgacctggct gatgacagtt tgatggaaac
     6181 gccgcatcgc atcgctaaaa tgtatgtcga tgaaattttc tccggtctgg attacgccaa
     6241 cttcccgaaa atcaccctca ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg
     6301 cgatatcact ctgaccagta cctgtgaaca ccattttgtt accatcgatg gcaaagcgac
     6361 ggtggcttat atcccgaaag attcggtgat cggtctgtca aaaattaacc gtatcgtgca
     6421 gttctttgcc cagcgtccgc aggtgcagga acgtctgacg cagcaaattc ttattgcgct
     6481 acaaacgctg ctgggcacca ataacgtggc tgtctccatt gatgcggtgc attactgcgt
     6541 gaaagcgcgt ggcatccgcg atgcaaccag tgccacaaca acgacctctc ttggtggatt
     6601 gttcaaatcc agtcagaata cgcgccacga gtttctgcgc gctgtgcgtc atcacaactg
     6661 attaaaaggc aggaaccatg gagcgcaacg taacgctcga ttttgttcgc ggcgtcgcca
     6721 ttctggggat cctgctatta aacatcagcg cctttgggct accaaaggcg gcttatctca
     6781 atcccgcctg gtacggcgct attacgccgc aggatgcatg gacctgggca tttctcgatc
     6841 tcatcggcca ggtgaaattc ctcacgcttt ttgcgctgct gtttggtgcg ggcctgcaaa
     6901 tgttgctgcc ccgtggcaga cgctggatcc agtcgcggtt aacgctgtta gtcttgctgg
     6961 gttttattca cggtttattg ttctgggacg gcgatattct gctggcttac gggctggtgg
     7021 gcttaatctg ctggcggctg gtgcgcgatg cgccatcggt aaaaagcctg tttaatacag
     7081 gcgtcatgct ttatctggtg gggcttggcg ttttgctgtt attggggctg atttccgaca
     7141 gccagaccag ccgcacctgg acgccggatg catcggctat tttgtatgaa aaatactgga
     7201 agcttcacgg cggcgttgaa gcgatcagta atcgtgccga tggtgttggc aacagtttac
     7261 tggcactggg cgcacagtat ggctggcaac tggcagggat gatgctcatt ggtgcggcgc
     7321 tgatgcgcag cggctggctg aaagggcagt tcagcttacg tcactatcgt cgtactggtt
     7381 ttgtgctggt ggcgattggg gtgaccatta accttcctgc catcgccctg caatggcagc
     7441 tggactgggc gtatcgctgg tgtgccttct tacttcaaat gccgcgggaa ctgagtgcgc
     7501 cgtttcaggc gattggctat gcgtcgctgt tttatggctt ctggccgcaa ttgagccgct
     7561 ttaagctggt gcttgcgatc gcctgcgtcg gacggatggc gctgaccaac tatctattgc
     7621 aaacgctgat ttgtaccatg cttttttacc acctcggttt gtttatgcat tttgaccgcc
     7681 tggagctgct ggcgtttgtt attccggtat ggctggcgaa tatcctcttc tctgttatct
     7741 ggctgcgtta cttccgccag gggccggtgg aatggctctg gcgtcagtta actttgcgtg
     7801 ctgccggaac ggcaatatct aaaacatcaa gataacgatc tggatcacat tcgttaacaa
     7861 aacggctgta accgtttcca ttgctgtgac tcgattcacg aagtcctgta ttcagtgctg
     7921 acaaaatagc cgccagcaag cagtcattta ctgcaatctc ataacaggta gtgaatatga
     7981 tcaccattcg tgatgtagcg cgttaggctg gcgtctctgt ggcaacggtt tcccgggtgc
     8041 tcaataacag cacgctggtc agtgccgaca cgcgtgaagc agtaatgaaa gccgtgagtg
     8101 agctggatta tcggccaaac gccaatgccc aggcgctggc aactcaggtt agcgacacca
     8161 ttggcgtggt ggtgatggac gtttctgatg cgtttttcgg cgcgctggta aaagcggtgg
     8221 atctagtcgc tcagcagcat cagaaatacg tgctaatcgg caatagctat catgaagcgg
     8281 aaaaagagcg tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgcg ttgattgttc
     8341 actcaaaagc attgagtgac gatgaactgg cgcaatttat ggataacatt cccggtatgg
     8401 tgttaatcaa ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg gataatctca
     8461 gcggtgcccg aatggcgacg cgcatgttgc tgaataacgg tcatcaacgt attggttatc
     8521 tttcttccag tcacggcatt gaagatgacg ccatgcgtaa agcaggctgg atgagtgcgt
     8581 tgaaagagca ggatattatt ccgccggaaa gctggattgg cactggtacg ccggacatgc
     8641 cgggcggtga ggcggcgatg gttgaactgc tggggcgcaa tctacaactt accgctatat
     8701 ttgcttataa cgacaatatg gccgctggcg cactgacagc attaaaagat aatggcattg
     8761 cgattccgtt acatctctca atcatcggtt tcgatgatat tcccatcgcc cgttacaccg
     8821 acccgcaatt aacgaccgtg cgttatccca ttgcttcaat ggctaaatta gccaccgaac
     8881 tggccttgca gggggcagca ggcaatattg atcctcgtgc cagccactgt tttatgccga
     8941 cgttagtgcg tcgccattct gtcgcaacgc gccagaatgc ggcggcgatc actaactcaa
     9001 caaatcaggc gatgtaaccg ctttcaatct gtgagtgatt tcacagtatc ttaacaatgt
     9061 gatagctatg attgcaccgt tttaacgttg taacccgtat gtaacagtga ataatcactt
     9121 tcaccgaggt aatagcgtca taacaacaat taaagccgtt ttctggagcg ttaccgggca
     9181 tggaagaacg aatttaaaaa agtgagcttc ggcgttcagt aacacttcat taactctact
     9241 gccccgccga gcatttatct caagcactac cctgcataag aaaaaccgga gataccatga
     9301 ataagaaggt gttaaccctg tctgctgtga tggccagcat gttattcggt gccgctgcac
     9361 acgctgctga tactcgcatt ggtgtaacaa tctataagta cgacgataac tttatgtctg
     9421 tagtgcgcaa ggctattgag caagatgcga aagccgcgcc agatgttcag ctgctgatga
     9481 atgattctca gaatgaccag tccaagcaga acgatcagat cgacgtattg ctggcgaaag
     9541 gggtgaaggc actggcaatc aacctggttg acccggcagc tgcgggtacg gtgattgaga
     9601 aagcgcgtgg gcaaaacgtg ccggtggttt tcttcaacaa agaaccgtct cgtaaggcgc
     9661 tggatagcta cgacaaagcc tactacgttg gcactgactc caaagagtcc ggcattattc
     9721 agggcgattt gattgctaaa cactgggcgg cgaatcaggg ttgggatctg aacaaagacg
     9781 gtcagattca gttcgtactg ctgaaaggtg aaccgggcca tccggatgca gaagcacgta
     9841 ccacttacgt gattaaagaa ttgaacgata aaggcatcaa aactgaacag ttacagttag
     9901 ataccgcaat gtgggacacc gctcaggcga aagataagat ggacgcctgg ctgtctggcc
     9961 cgaacgccaa caaaatcgaa gtggttatcg ccaacaacga tgcgatggca atgggcgcgg
    10021 tagaagcact gaaagcacac aacaagtcca gcattccggt gtttggcgtc gatgcgctgc
    10081 cagaagcgct ggcgctggtg aaatccggtg cactggcggg cacagtactg aacgatgcta
    10141 acaaccaggc gaaagcgacc tttgatctgg cgaaaaacct ggccgatggc aaaggtgcgg
    10201 ctgatggcac caactggaaa atcgacaaca aagtggtccg cgtaccttat gttggcgtag
    10261 ataaagacaa cctggctgaa ttcagcaaga aataagactg attgggcgca gtcgtttact
    10321 gcgcccttat tgtatggcca acaaggtaaa attatatggt cagctcaacg actccgtctt
    10381 ccggggaata cttgttggaa atgagcggta tcaacaagtc ctttcctggt gttaaggcac
    10441 ttgataacgt taatttaaaa ttccggccac attctatcca tgcattaatg ggggaaaacg
    10501 gtgcaggaaa atcgacatta ttaaaatgcc tgtttggtat ttatcaaaaa gactccggca
    10561 ccattttatt ccagggtaaa gagatcgatt tccattccgc caaagaggca ctggaaaatg
    10621 gtatttcgat ggtacaccag gagttaaacc tggtattaca acgttcggtg atggataaca
    10681 tgtggctggg gcgatatccc accaaaggca tgtttgtcga tcaggacaaa atgtaccgcg
    10741 aaaccaaagc gatttttgat gaactggata ttgatatcga tccgcgtgcg cgcgtcggca
    10801 cattatccgt ttcgcaaatg cagatgatcg aaatcgccaa agcgttttcc tataacgcga
    10861 aaattgtgat tatggatgaa ccgacttctt cgttaaccga aaaagaggtc aatcatctgt
    10921 tcactattat tcgtaaatta aaagagcgcg gctgcggtat tgtttatatc tcgcataaaa
    10981 tggaagaaat cttccagtta tgtgatgaag ttaccgtatt gcgcgacggt cagtggatcg
    11041 ccaccgaacc gctggcagga ctgacgatgg acaagatcat cgccatgatg gttgggcgtt
    11101 ctcttaatca gcgttttcct gacaaagaaa acaagccggg cgaagtcatc ctcgaggtgc
    11161 gtaacctaac gtcactgcgc cagccgtcga ttcgcgatgt ctcgtttgat ctgcataaag
    11221 gggagatcct cggtattgcc gggctggtgg gggcgaaacg taccgatatt gttgagacgt
    11281 tatttggtat tcgcgagaaa tcggctggca ccattacgtt gcacggcaaa cagatcaata
    11341 accataatgc caacgaagcc ataaaccacg gatttgcact ggtaactgag gagcgccgct
    11401 caacgggaat ttatgcctat ctggatattg gttttaactc gttaatttcc aatattcgca
    11461 actacaaaaa taaagttggt ttactggata actcgcggat gaaaagcgat acccagtggg
    11521 tgattgattc gatgcgggta aaaacgccgg gtcatcggac gcaaattggt tcgctctccg
    11581 gtggtaatca gcaaaaggtg attattggtc gctggctact aacgcaacca gaaatattaa
    11641 tgctcgatga accgacgcgc ggtattgatg tcggggcgaa gtttgaaatt tatcagttaa
    11701 ttgccgaact ggcgaagaaa ggcaagggga ttattattat ctcctctgaa atgcctgagt
    11761 tgttagggat aacagaccgt attctggtca tgagcaatgg tctcgtttcc ggaattgtcg
    11821 atacaaaaac aacaacgcaa aacgaaattc tgcgtcttgc gtctttgcac ctttaagatt
    11881 aggggcttcc catgagtgcg ttaaataaga aaagttttct tacttacctg aaagagggcg
    11941 gtatttacgt cgttctttta gttttgctgg cgattattat tttccaggac ccaacatttt
    12001 taagtctgtt gaacttaagt aatattctca cccagtcatc ggtgcgtatt attatcgcgc
    12061 tcggtgtggc agggttaatt gtcacccagg ggaccgatct ttctgctggt cgtcaggtag
    12121 ggctggcggc agtggtggct gcgacattat tgcagtccat ggataacgcc aacaaagtgt
    12181 tcccggaaat ggcgacgatg ccgattgcgc tggttattct gattgtctgt gccattggtg
    12241 cggtgatcgg tttgatcaac ggtctgatta tcgcttatct caacgtgacg ccgttcatta
    12301 ccacgctcgg cacgatgatc atcgtctatg gcatcaactc gctctattac gactttgtcg
    12361 gggcgtcgcc aatttctggt tttgacagtg gcttctctac ctttgctcag ggctttgtcg
    12421 cgctggggag tttccgtctc tcttacatca ccttctacgc gttgattgcg gtggcgttcg
    12481 tctgggtgtt gtggaacaaa acccgcttcg gtaagaacat ttttgccatt ggcggtaacc
    12541 cggaagcggc aaaagtatct ggtgtcaacg tcggcctgaa cctgctgatg atctacgcgt
    12601 tgtctggcgt gttctatgcc tttggcggga tgttagaagc cggacgtatc ggctctgcca
    12661 ccaacaacct cggctttatg tatgagctgg atgctatcgc ggcgtgcgtg gtaggcggtg
    12721 tatcgttcag cggcggtgtg gggacggtga ttggcgtggt gaccggggtg attattttta
    12781 ccgtcatcaa ctatggcctg acgtatatcg gcgtaaaccc atactggcag tacatcatca
    12841 aaggggcgat tattatcttc gccgtagcgc tggattcact gaaatacgcg cgtaagaaat
    12901 gatgtgaatg tggtccgcat tcggtcataa acgcacataa catcatgatc tgtgtggttt
    12961 tgtaggccgg ataaggcgtt cacgccgcat ccggcaacag gaaccaaatg ccggatgtga
    13021 cgcgggttct acaacgttac cgggtgttct ttctcgcctt tcttaaactt cacttccccg
    13081 agctcaatac aacccaccgg gcagacatga ccacaaagca gacaacccac acatttctcg
    13141 gtattacaat gcggtgtgcg ggttttctcg ctccattcca tcgcctggtg accgccgtcg
    13201 taacaggaaa tataacagcg tccacagcca acacatttgt caagattgat acggggatag
    13261 acaatataac tgcggtctaa atcttccgcc gggacaatat tgttattcgc cagacctacc
    13321 atctcctgca acgaatcaaa tccctgatcg gcgagataat gcgataaccc gctcgccata
    13381 tcttctacga tgcgatagcc gtactgcata atgccggtgg tgacctgtaa cgttgctgcg
    13441 ccgagcaata aaaactccgc cgcatcctcc caggtttcaa tgccgccgat accgctgatt
    13501 gggaaatcgc gcagttctgg atgggtgcgc atttgctgaa tgaagcgcag ggcgatcggt
    13561 tttaccgctt taccggaata tccggaaata cttgattttc cgttaacgat cggcataccg
    13621 attttctgat taagatcgat attggtgatg gatttaacgg tgttaatcgc cgcaatgcca
    13681 tctgcgccgc cgcgcttcgc cgccagcgcc acttcgcaca tatcaccgat attcggcgtc
    13741 atcttcgcca gcattggcag cgtggaaccc cgtttcactg cccgacaata tttttctacc
    13801 agctccgggc tttgcccgac atcgctaccc atcgcatgag aagtcatttg cggacaggag
    13861 aagttacact cgatcatatc cgcgccagct tcttgcacca ggcgcgccag ctcctcccat
    13921 tgctgctcat tttcccccat gatcgaagcg atcaatactt tgtccgggta atcttctttc
    13981 agccgacgca gggcggccag attctcctcc agcggatgct cggcaatctg ctccatattt
    14041 ttgaagccga taaaaccggt atcttctttc accagatgat caaaacgcgg cgagacttcg
    14101 ttggcgataa aaaagccgat cgttttaaac accacaccgc cccaacctgt gtcgtaggct
    14161 ttggcacaca tctcatagca gttgcctacc ggcgaagaag agaggcagaa cgggttggga
    14221 aacttcacgc cgcaaaaagt aatcgaaaga tctttcgtta acatgagcaa gctccctcta
    14281 aatagtgatg aatcgcctcg gcggcttctt tcccggtttt cacggcatag accacggttt
    14341 tgtctccctc aacaatatcg ccagcagcaa agacttgcgg gtcgcgggtc tggtaatttt
    14401 gtgttttgat ggtgttacgc tgcggctcta actccgcaaa ggcatctagt ctggcatgct
    14461 gaccgacggc gagaataatt ttatctgccg ccatcgtcag ttcgcccgat aaccgtacat
    14521 gcttaaacgt gactttattg ccttccacgg ctactggcgt gaatccatcg atgatcgaaa
    14581 cgcccagctc ccgggcgctg gtaaactctt tttcgcttgc cggaaactca tctaactctt
    14641 cacgcgctac gcaagttacc gcctgacagc cgagaacttt cagcgtgctg gctacgtcca
    14701 tcgcgacatc accgccgccg ataattaatg cgctttgtgg aatgctgata tcgccttgcg
    14761 cctgtcgtgc acgttgcaag aagtcgacgg caatctcaac gtcactatgc tcaaacagcg
    14821 gtagcccgga accgcttgat aaccccacgg tgaccagtac cgcgcggttt tctgctttca
    14881 gctgctcaag ggtgagtgtg ttaccgactt cgttgttgca cttaatggtc acgcccattt
    14941 tttcaatacg ggcgatctct gcatccagta ctgattgcgg taaccggaat tgcggaatgc
    15001 cgttacgcaa tcagccaccg ggttgtgctt ctttctcata gatcgtgacg tcataaccct
    15061 ggtttgtcag tgtcacactg gcctgcaatc cggcaggacc tgcgccaata atcgcgactt
    15121 tgccgagcgt tttagtaccg ggctgataaa tttccatccc ggtttgttgt tcaaaatcag
    15181 taacaaaacg ctgtaagcgg ccgatatcaa tgggagcatc aacaccggca cgggtacaac
    15241 cgctttggca taatttctcc gtcgggcaca ctctggcaca aacggcaccg agggcattat
    15301 tttcgcgaat tgtctcggca gcgcctttaa aattacgaaa gtagattgag cgaataaatt
    15361 tccccggatc ggtctgtgcc gggcaagcct gactacaggg agcgtcatga cataataaac
    15421 agcgagaggc ttctttaatc gccagtaaag gcgtaaaagc cggagtgagt tcatccagat
    15481 aattttgttg cggcatgtga tcttccttaa tgctatgagt aattaaggcg aacgctaaat
    15541 aaatattgcg tgctgatatt aaggctaagc gtgtggggaa atttaaactg tgaaaggatt
    15601 cacaaatagc tgcggttcag aatgttatat ccgcag
//
LOCUS       DAZRUF010000085        15511 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZRUF010000085 DAZRUF010000000
VERSION     DAZRUF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.085"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHXOL_002131"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHXOL_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR6809106.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHXOL_002132"
     CDS             567..650
                     /locus_tag="ACHXOL_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809107.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHXOL_002133"
     CDS             750..1571
                     /locus_tag="ACHXOL_002133"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR6809108.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHXOL_002134"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHXOL_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR6809109.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHXOL_002135"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHXOL_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR6809110.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHXOL_002136"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHXOL_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR6809111.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHXOL_002137"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHXOL_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR6809112.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHXOL_002138"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHXOL_002138"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR6809113.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHXOL_002139"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHXOL_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR6809114.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHXOL_002140"
     CDS             8347..9729
                     /locus_tag="ACHXOL_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR6809115.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHXOL_002141"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHXOL_002141"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR6809116.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHXOL_002142"
     CDS             complement(10485..10820)
                     /locus_tag="ACHXOL_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR6809117.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHXOL_002143"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHXOL_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR6809118.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHXOL_002144"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHXOL_002144"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR6809119.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPGKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHXOL_002145"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHXOL_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR6809120.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHXOL_002146"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHXOL_002146"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR6809121.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         3811 a         4021 c         3794 g         3885 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggtcgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatgg aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc agctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccggtaa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg g
//
LOCUS       DAZRUF010000086        15406 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZRUF010000086 DAZRUF010000000
VERSION     DAZRUF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.086"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..452
                     /locus_tag="ACHXOL_002147"
     CDS             <1..452
                     /locus_tag="ACHXOL_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR6809122.1"
                     /translation="LCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISL
                     PTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPP
                     FHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            668..757
                     /locus_tag="ACHXOL_002148"
                     /pseudo
     CDS             668..757
                     /locus_tag="ACHXOL_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(758..1816)
                     /gene="modC"
                     /locus_tag="ACHXOL_002149"
     CDS             complement(758..1816)
                     /gene="modC"
                     /locus_tag="ACHXOL_002149"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR6809123.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1819..2508)
                     /gene="modB"
                     /locus_tag="ACHXOL_002150"
     CDS             complement(1819..2508)
                     /gene="modB"
                     /locus_tag="ACHXOL_002150"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR6809124.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2508..3281)
                     /gene="modA"
                     /locus_tag="ACHXOL_002151"
     CDS             complement(2508..3281)
                     /gene="modA"
                     /locus_tag="ACHXOL_002151"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR6809125.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWSSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3447..3596)
                     /gene="acrZ"
                     /locus_tag="ACHXOL_002152"
     CDS             complement(3447..3596)
                     /gene="acrZ"
                     /locus_tag="ACHXOL_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR6809126.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3725..4513
                     /gene="modE"
                     /locus_tag="ACHXOL_002153"
     CDS             3725..4513
                     /gene="modE"
                     /locus_tag="ACHXOL_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR6809127.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4581..6053
                     /gene="modF"
                     /locus_tag="ACHXOL_002154"
     CDS             4581..6053
                     /gene="modF"
                     /locus_tag="ACHXOL_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR6809128.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6314..7330
                     /gene="galE"
                     /locus_tag="ACHXOL_002155"
     CDS             6314..7330
                     /gene="galE"
                     /locus_tag="ACHXOL_002155"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR6809129.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7340..8386
                     /gene="galT"
                     /locus_tag="ACHXOL_002156"
     CDS             7340..8386
                     /gene="galT"
                     /locus_tag="ACHXOL_002156"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR6809130.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8390..9538
                     /gene="galK"
                     /locus_tag="ACHXOL_002157"
     CDS             8390..9538
                     /gene="galK"
                     /locus_tag="ACHXOL_002157"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR6809131.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9532..10572
                     /gene="galM"
                     /locus_tag="ACHXOL_002158"
     CDS             9532..10572
                     /gene="galM"
                     /locus_tag="ACHXOL_002158"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR6809132.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10775..11527
                     /gene="gpmA"
                     /locus_tag="ACHXOL_002159"
     CDS             10775..11527
                     /gene="gpmA"
                     /locus_tag="ACHXOL_002159"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR6809133.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11686..12738)
                     /gene="aroG"
                     /locus_tag="ACHXOL_002160"
     CDS             complement(11686..12738)
                     /gene="aroG"
                     /locus_tag="ACHXOL_002160"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR6809134.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13054..13434
                     /locus_tag="ACHXOL_002161"
     CDS             13054..13434
                     /locus_tag="ACHXOL_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR6809135.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13548..14489
                     /gene="zitB"
                     /locus_tag="ACHXOL_002162"
     CDS             13548..14489
                     /gene="zitB"
                     /locus_tag="ACHXOL_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR6809136.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14486..15205)
                     /gene="pnuC"
                     /locus_tag="ACHXOL_002163"
     CDS             complement(14486..15205)
                     /gene="pnuC"
                     /locus_tag="ACHXOL_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR6809137.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15243..>15406)
                     /locus_tag="ACHXOL_002164"
     CDS             complement(15243..>15406)
                     /locus_tag="ACHXOL_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR6809138.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3915 a         4045 c         3850 g         3596 t
ORIGIN      
        1 gcctttgcat ggatcttgca ctggtaacag gacagcgcga aggagatctg gcggctatga
       61 gatgggagga tataagagat ggcaggctat acgtggaaca acaaaaaaca ggggcaaaaa
      121 ttcgcatttc tctccccacc acgatttcca gattgaattt aacacttgcc gatgtactgg
      181 ataacttgaa aaaaataaac ggtaaaaacg aaaagttact cggagggaag acagccagga
      241 caatcgcagc acagttcagg attgcccgag acacatccgg attgaaatgg gaaggtgacc
      301 caccgccatt tcatgaaatc cgaagtttat ccggtcgcct gcactcagca gaaaaaggcg
      361 gtgatttcac tcaggcgctc cttggacatc gttcatccag catgacggat aaataccgtg
      421 acggacgagg gagggaatgg aaagacattt agaattaatt tcgaatgatt tcgaaacacc
      481 acttgtaatt aattgattat aaatgattaa ttcctaatgg gcatttgctt catgaatgct
      541 cctttgcatt tagcaactga ctgtaacagc taaaattagt cgcttttggc ggtgaatgct
      601 gaattaaaac gcagaatttt gtccgatagt ggtatcggtg taccattcgc gcagacgaaa
      661 attcttaaat gacgcggtaa aagcgcgcgc caacattgtg attggtgata acaccaccga
      721 cagcattgcc cagttcattt atagccacct gatttaatca ggcggttatc gacacacttt
      781 taatttgcgc gtacagccac aggccaggtt tgatcgccag ttcatccctg gcccacgggc
      841 tgatacgcgc ccacagcgtt ttaccgccga cttccagctc cacttccacc tggccgttgt
      901 cgtcataact attaacaact tttgcccgca atacgttacg aatgctggtt tgctgcggcg
      961 gttgtaaaac cagagaaaca tcggaagcct gaatgcggat acgtagcgca gcttgcagcg
     1021 gttcgtccag cttattgacc cacaaatgct gatcgcccag cgccagcgcg gtcatcgcgt
     1081 aatgcggatg atgctcaagt accgtcactt tcagaatgct actttgttgc tctttcggca
     1141 gccacggatt catcacgcta ctgccccaca cttcctccag cgcgccaaag gctttcacct
     1201 gaccgttttc cagtaccatc actctgtctg ccagatggag gatctcatcc agcgaatggc
     1261 tgacatacaa catcggaatg ttgatttccc gtgtcagccg ttgcagataa ggcaacagtt
     1321 cgcgtttacg cggaatatcc agtgacgcca gcggttcatc cagcaacagc aattccggtg
     1381 ctgtcagcaa agcccgacca atcgccacgc gctgtttttc gcctccggac aggctgcctg
     1441 gtaaacggtc aagcaacggt tcaatgccta aaagcgccac cagcttatcg aactgatcga
     1501 ccatactttt cgacatgccg tagcgcagat tgccacgcac tttgtaatgc gggaacagcc
     1561 gcgcatcctg aaaaacatag ccaacgcgac gcttttccgg cgtcaggcag ataccttttt
     1621 cggcatcatt tagtacccgc ccattgagga caatccgccc tttttgcggg cgcgtcagtc
     1681 cactgatggc gttaatcagc gaagtttttc cggcaccgga gacgccaaag atagcagtga
     1741 tgccattggc gggcagtgtt tcattaatgg tcaggcaatg gttgcccaac gtctgggaaa
     1801 aattcagctc cagcatgatt agcgccccgc ccgttcacgg ctgattctgg ccagccattc
     1861 tgaaatcaac agggagatca tcgccagcgc aatagaaata atgcacagtc tcgccgcgcc
     1921 actttccccg ccgggggtct ggatcagggt atacatggca gagggaatgg ttcgcgtttc
     1981 gccaggaatg ttcgacacaa aggtgatggt tgcaccaaac tcaccgagag aacgagcaaa
     2041 agccagtacc gtaccaacaa taattcccgg taaggtcagc ggtaacgtga tagtaaagaa
     2101 aacgcgccag cgcccggcac ctagtgttct tgcggcctgt tcgagtttga catcaacccc
     2161 ttccagcgcc agacgaattg cccgcaccat cagcggaaac gacatgacgg cggcagcgag
     2221 aaccgcgccg cgccagctaa aggcgaaggt aataccaaac cagtcataca gacgttcacc
     2281 gataaatccg cgccgtccca tcgaaactaa taataagtaa ccgacgacca cgggcggtaa
     2341 caccagcggt agatgcagta cgctgtcgag cagagctttg cccggaaacg tgcaacgcac
     2401 cagtaaccag gcaaaaaaga tcccaaacgg caggctaaac agcacagcca gggaagaaac
     2461 tttcaggctt aataaaactg cctgccattc tggatcggtc agtatcatta cttggttgta
     2521 aatccgtaac gtttaaagat ttccgctgcc tgcggtccct tcagataatc ataaaatgct
     2581 ttcactgtcg cattgttatg cccttccaca acagcaaccg gatattccac ttttttatgt
     2641 gaatcttccg ggaaggtggc aaccactttt acccctttgc tgacaactgc gtcagaaccg
     2701 taaacaatgc ccaacggcgc ttcattgcgt tcgaccaacg ccagcgcgcc acgaacatct
     2761 tccgccggag ccagtttcgg ggagagcgta tcccatgcgc ccagtttttg cagtgcttct
     2821 tttgcataaa tgccagcagg gacatgttcc ggatcgccaa ccgcgaggcg accgccattc
     2881 agcagcgaac tccagttagt tgtgctgtcg atgatgaaat ctttctgctc gctggctttc
     2941 ggtgctacaa cgaccaggct attgccgagc agtgtctggc gcgaagccgt atcgatcgct
     3001 tttttatcaa ccgcataatc catccatttc tgatcggcag agataaacag atccgcaggc
     3061 gctcccgctt caatctgacg ggccagagta gatgacgaag cgaaagaaga aaccacatcc
     3121 acgccttttt cttttttata ctgcgtagca atgtcctgca ttgcgttagt cagtgatgcg
     3181 gcggcgaaca ccgtgatttt cccttcatct gccagtgtat tgccagcaac agcgaaagag
     3241 agtgccgccc cggcaaacaa gttcaaccat ttacgagcca ttggtaaccc cttaatgtaa
     3301 cgttatatag gtggcaatat aacggctagc tcagaatttt cccagcagtt attgctaacc
     3361 tttgtgaggt agataagaaa aaatatcggc tggaaaagca ggaagttgag aaaaagaaat
     3421 gcccgactaa gcgggcattc agggaatcaa tgattttgtc cgggctggtc ttttttacca
     3481 acaccagaaa agatgttgaa tacttcacca agaccgtaaa tcagacccag gatgatggcc
     3541 atcacgacag gtaccatgat tacggcgaat accagacttt ttaataactc taacatggtc
     3601 aactccagat atagtcatga gactattcta accgctaagc acagaaaagc actccccttt
     3661 tgtgcggtca gctttgcgtg gcgttcgttt tccgtcacaa taagactttt gccaggacat
     3721 tgttatgcag gccgaaatcc ttctcaccct taagctccaa caaaaattat tcgccgaccc
     3781 gcgccggatt tcgctactaa aacacattgc gctttccggt tccattagcc agggagcgaa
     3841 agatgccggt atcagctata aaagcgcctg ggatgccatt aacgagatga atcagttaag
     3901 cgagcatatt ctggttgagc gcgcaacagg cggtaaaggt ggcggcggcg cagtactgac
     3961 ccgctatggt cagcgactga ttcagctcta tgacttactg gcgcaaatcc agcaaaaagc
     4021 ctttgatgtg ttaagtgacg atgacgccct gccgctgaac agcctgctgg ccgcgatctc
     4081 acgtttttca ctgcaaacca gcgcccgtaa ccagtggttc ggtaccatca ccgcccgcga
     4141 tcatgatgac attcaacagc atgttgatgt cttactggct gacggaaaaa cacgcctgaa
     4201 agtcgcaatt accgcacaaa gcggcgcacg tctggggctg gatgaaggca aagaagtgtt
     4261 gatattgcta aaagcgccgt gggtaggcat tactcaggac gaagcggtcg cacaaaacgc
     4321 cgacaaccaa ttaccgggta ttattagtca tattgagcgc ggcgcagagc agtgcgaagt
     4381 attaatggcg ctacccgacg ggcaaacact gtgcgccaca gtgccggtaa atgaagcgac
     4441 ttctcttcag caagggcaga atgtcacggc ctactttaat gccgacagcg tgattatcgc
     4501 cacgctgtgc taagcgtgtt gacaatttgt tatgaaacac gtatccctgt cagtaatcgc
     4561 tgcacaaaat gggatataaa atgtcatcgt tgcaaatttt gcaaggcacg tttcgtctta
     4621 gcgacacaaa aacgctgcaa ttgcctcagc taacgttaaa cgcgggtgat agttgggcgt
     4681 ttgtcggttc gaatggaagc gggaaatcgg ccctggcccg cgcgctggcg ggggaacttc
     4741 cgcttttgaa aggtgaacgg caaagccagt tttcccacat cactcgtctc tccttcgagc
     4801 aattgcaaaa actcgtcagc gacgaatggc agcggaataa caccgatatg ctcggccctg
     4861 gcgaagatga caccggacgc actacggctg aaatcattca ggatgaagta aaggatgcac
     4921 cgcgttgcat gcaactggcg cagcagttcg gtattaccgc cctcctcgac cgacgcttta
     4981 aatacctttc cactggcgag acgcgaaaaa ccctgctgtg tcaggcgctg atgtcggagc
     5041 ctgacttgtt gattcttgat gagtcgttcg atggcctgga tgttgcctca cgtcagcagc
     5101 tggctgagcg actcgcctcg ttacatcagt ccggtattac tctggtactg gtgctcaatc
     5161 gcttcgatga gatcccggag tttgtccagt ttgctggcgt gctggcggat tgcacgttag
     5221 cggaaactgg cgctaaagag gaactgctcc aacaagcact cgtcgcgcaa ctggcgcata
     5281 gtgaacagct tgaaggtgtg caactgccgg agccggatga accttcagca cgtcacgcct
     5341 tacccgccaa cgaaccgcgc attgtgctga acaatggcgt ggtttcttat aacgatcgcc
     5401 ccattcttaa taaccttagc tggcaggtga atccaggtga acactggcaa attgtcgggc
     5461 caaatggtgc aggaaaatcg acgttattaa gcctggttac tggcgatcat ccgcaaggtt
     5521 acagcaacga tttgacgctt ttcggacgac gtcgcggcag cggcgaaacc atctgggata
     5581 tcaaaaagca tatcggttac gtcagcagta gtttgcatct ggattaccgg gtcagcacta
     5641 ccgtgcgtaa tgtgattctt tctggctatt ttgattcgat tggcatttat caggccgttt
     5701 cggatcgcca gcaaaaactg gtgcagcagt ggctggatat tctcggcatt gataaacgta
     5761 cggctgacgc tccgttccat agtctttcct ggggacagca gcgtctggcg ctgatcgtcc
     5821 gcgcactggt gaaacatccg acgttgctta ttctcgatga accactacag gggcttgatc
     5881 cgctcaatcg ccagcttatc cgccgttttg ttgatgtgct gattagcgaa ggtgaaacgc
     5941 aattgttatt tgtttcgcac cacgctgaag atgcgcctgc ctgtattacc catcgccttg
     6001 agttcgtgcc ggacggtgga ctctatcgct atgtgctgac aaaaatatat tgagtcggta
     6061 gtgctgacct tgccggaggc ggcctaagcg ccctctccgg ccaacggttc gacgcatgca
     6121 ggcatgaaac cgcttctttt ttcagataaa aagcgcaatc attcataaac cctctgtttt
     6181 ataatcactt aatcgcgcat aaaaaacggc taaattcttg tgtaaacgat tccactaatt
     6241 tattccatgt cacacttttc gcatctttgt tatgctatgg ttatttcata ccataagcct
     6301 aatggagcga attatgagag ttctggttac cggtggtagc ggttacattg gaagtcatac
     6361 ctgcgtgcaa ttactgcaaa acggtcatga tgtcatcatt cttgataacc tctgtaacag
     6421 taagcgcagc gtactgcctg ttatcgagcg cttaggcggc aaacatccaa cgtttgttga
     6481 aggcgatatt cgtaacgaag cgttgatgac cgagatactg cacgatcacg ctatcgacac
     6541 cgtgatccac ttcgccgggc tgaaagccgt cggcgaatcg gtacaaaaac cgctggaata
     6601 ttacgacaac aatgtcaacg gcactctgcg cctgattagc gccatgcgcg ccgctaacgt
     6661 caaaaacttt atttttagct cctccgccac cgtttatggc gatcagccca aaattccata
     6721 cgttgaaagc ttcccgaccg gcacaccgca aagcccttat ggcaaaagca aattgatggt
     6781 ggaacagatc ctcaccgacc tgcaaaaagc ccagccggac tggagcattg ccctgctgcg
     6841 ctacttcaac ccggttggcg cgcatccgtc gggcgatatg ggcgaagatc cgcaaggcat
     6901 tccgaataac ctgatgccat acatcgccca ggttgctgta ggccgtcgcg actcgctggc
     6961 gatttttggt aacgattatc cgaccgaaga tggtactggc gtacgcgatt acatccacgt
     7021 aatggatctg gcggacggtc acgtcgtggc gatggaaaaa ctggcgaaca agccaggcgt
     7081 acacatctac aacctcggtg ctggcgtagg cagcagcgtg ctggacgtgg ttaatgcctt
     7141 cagcaaagcc tgcggcaaac cggttaacta tcattttgca ccgcgtcgcg aaggcgacct
     7201 tccggcctac tgggcggacg ccagcaaagc cgaccgtgaa ctgaactggc gcgtaacgcg
     7261 cacactcgat gaaatggcgc aggacacctg gcactggcag tcacgccatc cacagggata
     7321 tcccgattaa ggaacgacca tgacgcaatt taatcccgtt gatcatccac atcgccgcta
     7381 caacccgctc accgggcaat ggattctggt ttcaccgcac cgcgctaagc gcccctggca
     7441 gggggcgcag gaaacgccag ccaaacaggt gttacctgcg cacgatccag attgcttcct
     7501 ctgcgcaggt aatgtgcggg tgacaggcga taaaaacccc gattacaccg ggacttacgt
     7561 tttcactaat gactttgcgg ccttgatgtc tgacacgcca gatgcgccag aaagcaacga
     7621 tcctttgatg cgttgccaga gcgcgcgcgg taccagccgg gtgatctgct tttcaccgga
     7681 tcacagtaaa acgttgccag aactgagcgt tgcggcattg acggaaatcg tcaaaacctg
     7741 gcaggagcaa accgcagagc tgggaaaaac gtacccgtgg gtgcaggtct ttgaaaacaa
     7801 aggtgcggcg atgggctgct ctaacccgca tccgcacgga cagatttggg caaatagctt
     7861 cctgcctaac gaagctgagc gcgaagaccg cctgcaaaaa gaatattttg ccgaacagaa
     7921 atcaccaatg ctggtggatt atgttcagcg cgagctggca gacggtagcc gtaccgttgt
     7981 cgaaaccgaa cactggttag ccgttgtgcc ttactgggct gcctggccgt tcgaaacgct
     8041 actgctgccc aaagcccacg ttttgcggat caccgatttg accgacgccc agcgcagcga
     8101 tttggcgctg gcgttgaaaa agctgaccag tcgttatgac aacctcttcc agtgctcctt
     8161 cccctactct atgggctggc acggcgcacc atttaatggc gaagagaatc aacactggca
     8221 gctgcacgcg cacttttatc cgcctctgct gcgctccgcc accgtacgta aatttatggt
     8281 tggttatgaa atgctggcag agacccagcg agacctgacc gcagaacagg cagcagagcg
     8341 tttgcgcgca gtcagcgata tccattttcg cgaatccgga gtgtaagaaa tgagtctgaa
     8401 agaaaaaaca caatctctgt ttgccaacgc atttggctac cctgccactc acaccattca
     8461 ggcgcctggc cgcgtgaatt tgattggtga acacaccgac tacaacgacg gtttcgttct
     8521 gccctgcgcg attgattatc aaaccgtgat cagctgtgca ccacgcgatg accgtaaagt
     8581 tcgcgtgatg gcagccgatt atgaaaatca gctcgacgag ttttccctca atgcgcccat
     8641 tgtcgcgcat gaaaactatc aatgggcgaa ctacgttcgt ggcgtggtga aacatctgca
     8701 actgcgtaac aacagcttcg gcggtgtgga catggtgatc agcggcaatg tgccgcaggg
     8761 tgccgggtta agttcttccg cttcactgga agtcgcggtc ggaaccgtat tgcagcagct
     8821 ttatcatctg ccgctggacg gcgcacaaat cgcgcttaac ggtcaggaag cagaaaacca
     8881 gtttgttggc tgtaactgcg ggatcatgga tcagctaatt tccgcactcg gcaagaaaga
     8941 tcatgccttg ctgattgact gccgctcact ggggactaaa gcagtttcca tgccgaaagg
     9001 tgtggctgtc gtcatcatca acagtaactt caaacgtacc ctggttggca gcgaatacaa
     9061 cacccgtcgt gaacagtgcg aaaccggtgc gcgtttcttc cagcagccag ctctgcgcga
     9121 tgtcaccatt gaagagttca acgctgttgc acatgagctg gacccaatcg tggcgaaacg
     9181 cgtgcgtcat atcctgactg aaaacgcccg caccgttgaa gctgccagcg cgctggagca
     9241 aggcgatctg aaacgtatgg gcgagttgat ggcggagtct catgcctcta tgcgcgatga
     9301 tttcgaaatc accgtgccgc aaattgacac tctggtagaa atcgtcaaag cagtgattgg
     9361 cgacaaaggt ggcgtacgca tgaccggcgg cggatttggc ggctgtatcg tcgcgctgat
     9421 cccggaagag ctggtgcctg ccgtacagca agctgtcgct gaacaatatg aagcaaaaac
     9481 aggtattaaa gagacttttt acgtttgtaa accatcacaa ggagcaggac agtgctgaac
     9541 gaaactcccg cactggcacc cgatggtcag ccgtaccgac tgttaacttt gcgtaacaac
     9601 gcagggatgg tagtcacgct gatggactgg ggtgcgactt tactttcagc tcgtattccg
     9661 ctttccgatg gcagcgtccg cgaggcgctg ctcggctgtg ccagcccgga atgctatcag
     9721 gatcaggccg cgtttctggg ggcctctatt ggtcgttatg ccaaccgcat cgccaatagc
     9781 cgttatacct ttgacggtga aacggtgacg ctttcgccaa gtcagggcgt taaccagctg
     9841 cacggcggcc cggaagggtt cgacaaacgt cgctggcaga ttgtgaacca gaacgatcgt
     9901 caggtgctgt ttgccctgag ttcagatgat ggcgatcagg gcttcccggg taatctcggc
     9961 gcgacggtgc aatatcgtct gaccgacgat aaccgtatct ccattactta tcgcgccaca
    10021 gttgataaat cttgcccggt gaatatgact aatcacgtct atttcaatct tgacggcgag
    10081 cagtctgacg tgcgcaatca caagttgcag attctggcgg gcgaatatct gccggttgat
    10141 gaaggcggca ttccgcacga cggcctgaaa tctgtcgccg gaacctcttt tgatttccgc
    10201 agcgccaaaa tcatcgccag tgagtttctt gccgacgacg atcagcgcaa agtgaaaggt
    10261 tacgatcacg cattcttgtt acaggccaaa ggcgatggca agaaagtggc ggcgcatgtc
    10321 tggtcagcag atgaaaaatt gcagctgaag gtctacacca ccgctcccgc tcttcaattc
    10381 tactccggca acttcctcgg cggcacaccg tcgcggggaa ccgaacctta cgccgactgg
    10441 caaggactgg cgttggaaag cgagtttctg ccggacagcc cgaaccaccc tgaatggccg
    10501 caaccggact gcttcctgcg tcctggcgaa gagtattcca gcctgacgga atatcagttt
    10561 attgctgagt aatgttaagc cctcgtcatg agggctttat cttcatattg ttcaaatcac
    10621 cagcaaacac cgacatattt gcaactcaat attcacaaca accttacact gcgccactat
    10681 tttcgctatg gttatgcgta agcattgctg ttgcttcgtc acggcaatat aatgagaatt
    10741 attatcataa aaagataatt tgaggagtaa gtatatggct gtaactaagc tggttctggt
    10801 tcgtcatggc gaaagtcagt ggaacaaaga aaaccgtttc accggttggt acgacgtgga
    10861 tctgtctgag aaaggcgtaa gcgaagcaaa agcagcaggt aagctgctga aagaggaagg
    10921 ttacagcttt gactttgctt acacttctgt gctgaaacgc gctatccata ccctgtggaa
    10981 tgtgctggac gaactggatc aggcatggct gcccgttgag aaatcctgga aactgaacga
    11041 acgtcactac ggtgcgttgc agggtctgaa caaagcggaa accgccgaaa agtatggcga
    11101 cgagcaggtg aaacaatggc gtcgtggttt tgccgtgact ccgccggaac tgactaaaga
    11161 tgatgagcgt tatccgggtc acgatccgcg ttacgcgaaa ctgagcgaga aagagctgcc
    11221 gctgactgaa agcctggcgc tgaccattga ccgcgtgatc ccttactgga atgaaactat
    11281 tctaccgcgt atgaagagcg gtgagcgcgt gatcatcgct gcacacggta actctttacg
    11341 tgcgctggtg aaatatcttg ataacatgag tgaagaagag attcttgagc tgaatatccc
    11401 gaccggcgtg ccgctggtgt atgagttcga cgagaatttc aaaccgctga aacgctatta
    11461 tctgggtaat gctgacgaga tcgcagcgaa agcagcggcg gttgctaacc agggtaaagc
    11521 gaagtaaacg tcattcgttt aaaaatgaga aagccgactg caagtgagtc ggcttttttg
    11581 ttgctaacaa tggagcacat tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg
    11641 attcatcgga tacgccactc tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc
    11701 ttttactgca ttcgccagtt gacgtaacag agcatcggta tcttcccagc caatgcaggc
    11761 atcggtgatg ctcttaccgt aggccagcgg ctccccgcta tcgaggctct gattgccttc
    11821 caccagatgg ctttccacca tcacgccaat aatggccttt tcgccaccgg caatctgctg
    11881 gcaaacgtca gcacaaacat ccatctgctt tttgaattgt ttggacgagt tagcatggct
    11941 gaaatcgatc atcacctgtg ctggcaggcc tgctttgttc agcccttctt tcacttcagc
    12001 aacgtgcttc gcgctgtagt taggctcttt accgccgcgc agaatgatat ggcaatcacc
    12061 gttaccgctg gtattcacaa tcgccgaatg cccccattta gttacggaca ggaagcagtg
    12121 cggcgcaccg gcggcattaa tggcatcgat agccacttta atcgtaccgt ctgtgccatt
    12181 tttaaaacct accggacaag aaagaccaga cgccagttcg cggtgcacct gcgattccgt
    12241 ggtacgtgca ccaattgcgc cccagctcat caggtcagcg agatactgag gagtgatcat
    12301 atcgagaaac tcacccgccg ctggcagacc gctgtcgtta atatcaagca gcaatttacg
    12361 ggctatacgc agaccgtcgt tgatctggaa gctgttatcc atatgcggat cgttaatcaa
    12421 ccctttccag cccaccgtgg tacgcggctt ttcaaaatag acgcgcatta cgatttccag
    12481 ctcatctttc agctcttcac gcagcgccag caagcgagtg gcatactctt tagccgcgac
    12541 aggatcatga attgagcacg ggccaatcac caccaacagg cgatcatcat tacctttcag
    12601 gatcttatgg atcgcttttc gggcatgggc aaccgtattc gcggcatttt cagttacggg
    12661 gaatttttcc agcaatgcga caggaggaag taactctttg atttctttga tgcgtaaatc
    12721 gtcgttctga taattcatgt ctgttccagt gttgccatac ttatcttagt gaatgcaata
    12781 cttccaatct atatcttccg tcagaatgtg taaacggggt tttacactat gaacggatta
    12841 atcctggaat tagcaagaaa aacgccagat tgtcgcgaaa aacgagatct ctcctacaat
    12901 ttctaactgt aactcctttg tttgttaatt atttcaagat tctctgctgc gtttcataaa
    12961 ctggctgaaa tcttaaacca atgccttata ttcacctgca aatgcactgt tggaagaggt
    13021 tatccgacat aacgaccata acaggagcat cctatgaaaa tgacaaaact ggccacatta
    13081 tttctgactg ccactctaag ccttgccagc ggtgccgcac tggctgccga tagcggagcg
    13141 caaactaata acggccaggc aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc
    13201 cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg taaataccgg ttctggcggc
    13261 acaatgctgc attcggatgg ttcttcaatg aacaatgacg gaatgaccaa agatgaagag
    13321 cacaagaaca ccatgtgcaa agatggtcga tgtccggata tcaataaaaa ggtacaaatc
    13381 ggtgatggca tcaataatga tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc
    13441 aaaatagcca ataatgggag agcggagtct ctccctttta atttttcact tctaaaagca
    13501 gtatgatgtc ataaagttaa gtgagaataa ttaagggatg actcgtcatg gcgcactcac
    13561 actcacacac gtcctcacac ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt
    13621 tcggcgtgac tgccgggttt atgctggtag aagtcgttgg tggttttctt tctggttctc
    13681 tggcattgct ggccgatgcg ggtcatatgt tgaccgatac tgccgccctg cttttcgccc
    13741 tactcgccgt gcaattttca cgtcgtcctc ccaccattcg ccacactttc ggttggctaa
    13801 gactgaccac gctcgcggcg tttgtgaatg ccatcgccct cgtggtaatt accattttga
    13861 ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc ggtcgagggc ggcatgatga
    13921 tggcaattgc cgtggctggg ttgctggcaa atatactttc tttctggtta cttcatcacg
    13981 gcagcgaaga gaaaaacctc aacgtacgag cagcggcatt gcatgtgctc ggggacctgt
    14041 tgggttcggt tggggctatt attgccgcgt tgattattat ctggaccggc tggacacctg
    14101 ctgaccccat tctctcgata gtggtgtcgc ttctggttct gcgtagcgcg tggcgactgt
    14161 tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt atcgctggat atcgctgaac
    14221 tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat
    14281 ggatggtagg cgagaagccg gtgataacgc tgcatgtgca ggtgatcccg ccacacgatc
    14341 acgatgcctt gttggatcag atccaacact acctgatgga tcactatcag attgagcacg
    14401 ccaccattca gatggaatat caaccttgtc atgggccaga ctgccacctg aatgaagggg
    14461 tgtctggcca ttcacatcat caccattaat gggacagcgc gcgtgagcct ctttcacgtg
    14521 cgctgttgat ccacatccga ctgccgttga gcgcaataaa ggtcaggatg atgtactcca
    14581 gagacatggc gtaaacgccc tgaagtgcaa aaataacgac gctaatcacg ttaataatca
    14641 cccacaacag ccagttttcc acatacttac gcgtcatcag aatcattgcc acgatagata
    14701 acaccatcat gcatgaatcc cagaacggga aagcgtccgg ttgcagttca ggcatcacaa
    14761 cctgtaatcc taatgcttgc atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg
    14821 gattgataaa taccgtcatc agaccaatcg aaacaacgca aaccgccaac cagctgagcg
    14881 ccttcggcaa tggtaaccag cgaattttca actccgcctc gttctgactg gtttgtcgcg
    14941 accacgcata ccaaccgtaa atattcgcgg caaagaaaaa cacctgtaat agcaggctgg
    15001 catacagctg aatctgaaag aaaataatgc caaacaaggt gacgttgatc aggccaaaga
    15061 agtagttgct gatcttctcc agactggcaa ggccaataca cagcaacccg gcgatcgtgc
    15121 ctaccgcttc gatccatgag agatcataac cgcctgcccc tattggtata tgtaccagga
    15181 tattctgcac actaaaaaaa tccatctttc ccccaaagcg taacgttacg ccttattatt
    15241 cgttatccac gtagtgtagc cgcaaaatcc agcatacgat tgagcggcac cagcgccctc
    15301 tctcgcagcc tttcatcaag aacctcgtga ttgcttcctt cctgttctaa tgcctctgcg
    15361 atggactgaa ggccattcat ggccatccac ggacaatgcg cgcagc
//
LOCUS       DAZRUF010000087        15395 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZRUF010000087 DAZRUF010000000
VERSION     DAZRUF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.087"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHXOL_002165"
     CDS             <1..1015
                     /locus_tag="ACHXOL_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR6809139.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHXOL_002166"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHXOL_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR6809140.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHXOL_002167"
     CDS             1797..1904
                     /locus_tag="ACHXOL_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR6809141.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHXOL_002168"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHXOL_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR6809142.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHXOL_002169"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHXOL_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR6809143.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHXOL_002170"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHXOL_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR6809144.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHXOL_002171"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHXOL_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR6809145.1"
                     /translation="MPLSKVWADSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHXOL_002172"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHXOL_002172"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR6809146.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="ACHXOL_002173"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="ACHXOL_002173"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR6809147.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHXOL_002174"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHXOL_002174"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR6809148.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="ACHXOL_002175"
     CDS             complement(11226..11813)
                     /locus_tag="ACHXOL_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR6809149.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="ACHXOL_002176"
     CDS             11983..12561
                     /locus_tag="ACHXOL_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR6809150.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="ACHXOL_002177"
     CDS             complement(12691..13452)
                     /locus_tag="ACHXOL_002177"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR6809151.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHXOL_002178"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHXOL_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR6809152.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15165..15248
                     /gene="yohP"
                     /locus_tag="ACHXOL_002179"
     CDS             15165..15248
                     /gene="yohP"
                     /locus_tag="ACHXOL_002179"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR6809153.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3751 a         4202 c         3856 g         3586 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag atgatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaatctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgcaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aggcatcact ttaccggttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgccaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accagcaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcagt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    15241 tattttaaaa ttaattaatg tcatcaggtc cgaaaataac gagaatattt cagtctctca
    15301 tcctgttgcg ctcctgtcat gtgcattgct tcatataatc actggcgcaa ggagcgcgca
    15361 gggggcggcc aatcgccgcc gccccctgcc ccccc
//
LOCUS       DAZRUF010000088        15231 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZRUF010000088 DAZRUF010000000
VERSION     DAZRUF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.088"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHXOL_002180"
     CDS             267..1925
                     /locus_tag="ACHXOL_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR6809154.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHXOL_002181"
     CDS             complement(1922..2878)
                     /locus_tag="ACHXOL_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR6809155.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHXOL_002182"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHXOL_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR6809156.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHXOL_002183"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHXOL_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR6809157.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHXOL_002184"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHXOL_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR6809158.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHXOL_002185"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHXOL_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR6809159.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHXOL_002186"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHXOL_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR6809160.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHXOL_002187"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHXOL_002187"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR6809161.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="ACHXOL_002188"
     tRNA            complement(9740..9827)
                     /locus_tag="ACHXOL_002188"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            10061..10999
                     /gene="ghrA"
                     /locus_tag="ACHXOL_002189"
     CDS             10061..10999
                     /gene="ghrA"
                     /locus_tag="ACHXOL_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR6809162.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            11054..11791
                     /gene="ycdX"
                     /locus_tag="ACHXOL_002190"
     CDS             11054..11791
                     /gene="ycdX"
                     /locus_tag="ACHXOL_002190"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR6809163.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            11815..12369
                     /gene="ycdY"
                     /locus_tag="ACHXOL_002191"
     CDS             11815..12369
                     /gene="ycdY"
                     /locus_tag="ACHXOL_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR6809164.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            12471..12962
                     /locus_tag="ACHXOL_002192"
     CDS             12471..12962
                     /locus_tag="ACHXOL_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR6809165.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(13027..13860)
                     /gene="csgG"
                     /locus_tag="ACHXOL_002193"
     CDS             complement(13027..13860)
                     /gene="csgG"
                     /locus_tag="ACHXOL_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR6809166.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPQAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(13887..14303)
                     /gene="csgF"
                     /locus_tag="ACHXOL_002194"
     CDS             complement(13887..14303)
                     /gene="csgF"
                     /locus_tag="ACHXOL_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR6809167.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(14328..14717)
                     /gene="csgE"
                     /locus_tag="ACHXOL_002195"
     CDS             complement(14328..14717)
                     /gene="csgE"
                     /locus_tag="ACHXOL_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR6809168.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKKDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(14722..>15231)
                     /gene="csgD"
                     /locus_tag="ACHXOL_002196"
     CDS             complement(14722..>15231)
                     /gene="csgD"
                     /locus_tag="ACHXOL_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR6809169.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
BASE COUNT         3747 a         3549 c         4073 g         3862 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgtcgttcc
     9841 tgtcttgctg gaacgggcgc taatttaggg aaatcatgac ctgaggtcaa caaacttttt
     9901 gaaaaaatcg cgcgtttatt caaacttcaa tcaatgtgtg gttttaataa gcgaaatctg
     9961 ctttttttgc caccgaccac ggatttgtta tgctggtggc ctttgtagat cataacgata
    10021 agtgcgaata aatttcgcac aacgcttttc gggagtcagt atggatatca tcttttatca
    10081 cccaacgttc gatacccaat ggtggattga ggcactgcgc aaagctattc ctcaggcaag
    10141 agtcagagca tggaaaagcg gagataatga ctctgctgat tatgctttag tctggcatcc
    10201 tcctgttgaa atgctggcag ggcgcgatct taaagcggtg ttcgcactcg gggccggtgt
    10261 tgattctatt ttgagcaagc tacaggcaca ccctgaaatg ctgaaccctt ctgttccact
    10321 ttttcgcctg gaagataccg gtatgggcga gcaaatgcag gaatatgctg tcagtcaggt
    10381 gctgcattgg tttcgacgtt ttgacgatta tcgcatccag caaaatagtt cgcattggca
    10441 accgctgcct gaatatcatc gggaagattt taccatcggc attttgggcg caggcgtact
    10501 gggcagtaaa gttgctcaga gtctgcaaac ctggcgcttt ccgctgcgtt gctggagtcg
    10561 aacccgtaaa tcgtggcctg gcgtgcaaag ctttgccgga cgggaagaac tgtctgcatt
    10621 tctgagccaa tgtcgggtat tgattaattt gttaccgaat acccctgaaa ccgtcggcat
    10681 tattaatcaa caattactcg aaaaattacc ggatggcgcg tatctcctca acctggcgcg
    10741 tggtgttcat gttgtggaag atgacctgct cgcggcgctg gatagcggca aagttaaagg
    10801 cgcaatgttg gatgttttta atcgtgaacc cttaccgcct gaaagtccgc tctggcaaca
    10861 tccacgcgtg acgataacac cacatgtcgc cgcgattacc cgtcccgctg aagctgtgga
    10921 gtacatttct cgcaccattg cccagctcga aaaaggggag agggtctgcg ggcaagtcga
    10981 ccgcgcacgc ggctactaat aaagcatcag gattcctgct atccttggcg ggaattgaat
    11041 acaggagaga gttatgtatc ccgtcgacct tcatatgcat accgttgcca gcacacatgc
    11101 atatagcaca ttgagtgatt acattgccca ggccaaacaa aagggcatta aactttttgc
    11161 gatcaccgat catggcccgg atatggaaga tgctccgcat cactggcact tcattaacat
    11221 gcgtatctgg ccgcgagtgg ttgatggggt agggatcctg cgcggcatcg aagctaacat
    11281 taaaaatgtt gatggtgaaa ttgattgcag cggtaaaatg tttgactcgc tggatctgat
    11341 tattgccggt tttcatgagc cggtttttgc accacatgac aaagcgacca atacacaagc
    11401 gatgatcgcc actatcgcca gcggcaatgt gcatataata agccatcccg gaaatcccaa
    11461 atatgaaata gatgtgaaag ccgttgctga agcagccgcg aaacatcagg tggcgctgga
    11521 aatcaataat tcttcatttt tacactcacg taagggcagt gaagacaact gtcgtgcggt
    11581 agccgcagcg gtacgtgatg ctggaggttg ggtggcatta ggctcggatt ctcacaccgc
    11641 gtttaccatg ggggaatttg aagagtgtct taaaatcctc gacgcggtag attttccgcc
    11701 agagcgcatt ttgaatgttt ctccgcgccg cttactgaac ttccttgaat ctcgcggtat
    11761 ggcaccgatt gcggaatttg cagaccttta attactagta atggaaatat attaatgaac
    11821 gagttttcta tcctctgtcg tgtgctgggt tcgctctatt accgccaacc gcaagatcct
    11881 ttactggtgc cgctgtttac cctgattcgt gaggggaaac tggctgcgaa ctggccactg
    11941 gagcaggatg agttactgac gcgtttacag aaaagctgtg atatggcgca agtctctgcc
    12001 gattacaatg cgttgtttat cggcgatgaa tgtgcagtgc cgccatatcg tagcgcatgg
    12061 gttgaggacg taacggaagc ggaagtgcgc gcttttcttt ccgaacgagg gatgccactg
    12121 gcggatacgc cagccgatca catcggcaca ttgctgcttg cggcttcctg gctggaagat
    12181 cagtcaacgg aagatgagag cgaagcactg gaaacactgt tcagtgagta tctgttaccc
    12241 tggtgtggtg cgttcctcgg caaagtggag gcccatgcaa ccacgccttt ctggcgcacc
    12301 atggcaccgc taacccgtga tgccattagt gcaatgtggg acgagctgga agaagattct
    12361 gaagagtaaa tgtgatcatc gtcactcata actgcaacag ataatttgtt gttgcataaa
    12421 atgtgtgctc gatctcattc atggccgcgt tttctgctat catgcgcggc atgaacatac
    12481 ttctctctat tgcaatcaca acaggcattc tctccggtat ctggggatgg gtggctgttt
    12541 ccctgggctt actaagctgg gcgggcttct tgggctgtac ggcctacttt gcctgcccgc
    12601 aaggtgggct gaaagggctg gcgatctccg ctgcaaccct gcttagtggc gtggtgtggg
    12661 cgatggtcat tatttacggt agtgcactgg caccacatct ggaaattctc ggttatgtca
    12721 taaccggtat tgtggcgttt ctgatgtgta ttcaggccaa acagctgctg ctttcatttg
    12781 ttccggggac gtttataggc gcatgcgcga cgtttgccgg gcagggtgac tggaaactgg
    12841 tgttaccttc gctggcgctt gggctggtat ttggttacgc aatgaaaaac agcggcctgt
    12901 ggctggcggc gcgtagtgca aagacggctc accgtgagca ggaaatcaaa aataaagcgt
    12961 gaggggcact cacgctttcg cttaaacagt aaaatgccgg attattaatt ccggcatttt
    13021 tatctgtcag gattctggtg gaaccgacat atggcggtat ttaaccagaa tgtcattctg
    13081 ccgttctgct ttgttctgca aatcccacag accacggtcg ataccatcat taatcaggaa
    13141 aatgacccct gtttcgatag ccgacatcag acacagcata acgggttcgt ttgaggtgta
    13201 acccacttcc ccttccagca agcgctggta gtcaataaag cggaaaaccc cggcctgaac
    13261 ttcataggaa agtatcgtct tactggtgtt caccgaggag aggatctcac cggtactcac
    13321 attgacgaca cgcaggttca cggcaatctg atcgagctgg tattgcgtgt ctgcaccgat
    13381 cccaaaatat cttgccccaa caccgcccga tttaacgttg ctttcataac cgataatcga
    13441 accttcaacc atgatatttg ccgccgttaa agattgcagc gggattcggt tattaatggc
    13501 aactgtgccg ttttcttgtg ccgcacgaat aatcttgcgc tcgttaagca ggttttgtaa
    13561 actctgacgc tccagcggta taaaccagcg agaatctttc aatgccgtga ccagcattgc
    13621 cgtggcgctt tgcggaacag cagtggagaa gttacttgcc gggtagggtt taaattgccc
    13681 ggtttcgtcc tgaatgttgt ataccgaaac aaagatctta cccgtcggcg ctggcagatg
    13741 ggtcaaatct ttgtagctct gagcttgagg cattaatgtc ggtctggcgg cttctttagg
    13801 cggggcggtt aagcatccgc tcagtaacat gacggcaacc aaaagaaata agcgctgcat
    13861 gattattttc cttatgaagc tggggcttaa aaatcggttg agttattttg taaacccgaa
    13921 acctggatgg tcgaggtttg tccggtttta cgatctgtca cgttcaactg caattgacca
    13981 tcgcggttag caatatcaac aatataatcg ttggtcacca tgcggcccgg tttaccggta
    14041 ttaatattcg acagtagccc acctaaaatt tgtgactgga tggcctgagt aaagttatct
    14101 aacgctgagg gtgtttcaat accaaagtca tcgttatagc tcggatcttt ataagagttt
    14161 tgggcctgag cgctatttaa taaaaaagcg ccattatttg ggttaccacc aaagtttgga
    14221 ttacggaact ggaaagtcat ggttccagcc caacttaatg gcgaaataag catgagtaga
    14281 actactgcat gtttgacacg cattgcagcc tccgaacaat tttttattta gaattcatca
    14341 tgcgccaaat cgcccgtact taataacgcc tgatttatct ggcgacgatt tagtgcttct
    14401 tcagtttgaa tcagtgcaaa gacgacagtt ttctcgaagt cttttttcaa cggaaataaa
    14461 aaagtctgga aaataacgtc ctgattgacc gttatagtga tccagcttcc ccatcgtgca
    14521 ctgggccttt cattaatcgt taagttaccc gtatagtcac tttcccattt atcactaaag
    14581 gctcggtaaa aatcatggcc aatagatgaa acagtatggt cagttagcaa tcccgggact
    14641 tctacctcaa cggcgtgaag attccctgcg gcgaacagaa attctgccgc cacaatccag
    14701 cgtaaataac gtttcatggc tttatcgcct gaggttatcg tttgcccagg aaaccgcttg
    14761 tgtccggttt tttacggcta tcttcttgaa aagattataa agatgcgttt taaccgtatt
    14821 ttcgctgatg aacaacgaac gagcgatctc gttattagac gcgccgatac gcagtttatt
    14881 caggatctct ttttcccgat gagtaaggag ggctgattcc gtgctgttat aacgatagtt
    14941 acctgaatgc gtaatcaagt agctggcaag cttttgcgta aagtagcatt cgccgcgcag
    15001 gacgccttgc aacccattga caacacgttc ttgatcctcc atggcataaa aaacgccgtt
    15061 gatatgaggc cagttttcaa tgtcgcggta cgggtaatct tcaggcgtat ttagcaacaa
    15121 tattttgata ttgttgtttt tcctgctcaa agtatcctgc caataatgga taagcttttt
    15181 atccgcttcc atcatatcca gaagaataat agagcctgaa gatatatcgt c
//
LOCUS       DAZRUF010000089        15169 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZRUF010000089 DAZRUF010000000
VERSION     DAZRUF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.089"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHXOL_002197"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHXOL_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR6809170.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHXOL_002198"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHXOL_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR6809171.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHXOL_002199"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHXOL_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR6809172.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHXOL_002200"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHXOL_002200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR6809173.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHXOL_002201"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHXOL_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR6809174.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHXOL_002202"
     CDS             complement(8214..9107)
                     /locus_tag="ACHXOL_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR6809175.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHXOL_002203"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHXOL_002203"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR6809176.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHXOL_002204"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHXOL_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR6809177.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHXOL_002205"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHXOL_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR6809178.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHXOL_002206"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHXOL_002206"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR6809179.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHXOL_002207"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHXOL_002207"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR6809180.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..>15169)
                     /locus_tag="ACHXOL_002208"
     CDS             complement(14275..>15169)
                     /locus_tag="ACHXOL_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR6809181.1"
                     /translation="FSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQV
                     YMLDVGQGLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEH
                     LDHRGGLDSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGN
                     NHSCVVKVDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLI
                     QRVNGKVALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISS
                     LREQILPRWYHQWFGVPVDNG"
BASE COUNT         3559 a         4283 c         3740 g         3587 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtt atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaat
//
LOCUS       DAZRUF010000090        15048 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZRUF010000090 DAZRUF010000000
VERSION     DAZRUF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.090"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(78..2357)
                     /gene="maeB"
                     /locus_tag="ACHXOL_002209"
     CDS             complement(78..2357)
                     /gene="maeB"
                     /locus_tag="ACHXOL_002209"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR6809182.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2646..3596
                     /gene="tal"
                     /locus_tag="ACHXOL_002210"
     CDS             2646..3596
                     /gene="tal"
                     /locus_tag="ACHXOL_002210"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR6809183.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3616..5619
                     /gene="tkt"
                     /locus_tag="ACHXOL_002211"
     CDS             3616..5619
                     /gene="tkt"
                     /locus_tag="ACHXOL_002211"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR6809184.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5715..6758)
                     /locus_tag="ACHXOL_002212"
     CDS             complement(5715..6758)
                     /locus_tag="ACHXOL_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR6809185.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6884..7459)
                     /gene="nudK"
                     /locus_tag="ACHXOL_002213"
                     /pseudo
     CDS             complement(6884..7459)
                     /gene="nudK"
                     /locus_tag="ACHXOL_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
     gene            complement(7527..9506)
                     /gene="aegA"
                     /locus_tag="ACHXOL_002214"
     CDS             complement(7527..9506)
                     /gene="aegA"
                     /locus_tag="ACHXOL_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR6809186.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALEQNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9712..11412
                     /gene="narQ"
                     /locus_tag="ACHXOL_002215"
     CDS             9712..11412
                     /gene="narQ"
                     /locus_tag="ACHXOL_002215"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR6809187.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11576..14689
                     /gene="acrD"
                     /locus_tag="ACHXOL_002216"
     CDS             11576..14689
                     /gene="acrD"
                     /locus_tag="ACHXOL_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR6809188.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14788..14847)
                     /gene="ypfM"
                     /locus_tag="ACHXOL_002217"
     CDS             complement(14788..14847)
                     /gene="ypfM"
                     /locus_tag="ACHXOL_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR6809189.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3524 a         3991 c         3932 g         3601 t
ORIGIN      
        1 taataagctt tactaatata ttgcagccaa accgcttacc ctgaatattc agggtaagcg
       61 tgagagttaa aaaagaatta cagcggttgg gtttgcgctt caaccacggc cagcgccacc
      121 atgttgacga tacgacgcac cgatgcgatc ggcgttaaca cgtgaaccgg tttcgccacg
      181 cccatcagca ccggaccgac agtcacacct tccgagctgg aaacgcgcag taagttgtaa
      241 ctaatgcggg cagcttccat gttcggcatc accagaatat tggcagaacc tttcaaaggg
      301 ctgtccggca tacggtcgtt gcgaatcgct tccaccagcg ccgcatcgcc gtgcatttca
      361 ccatcaatca tcagttctgg cgcacgttcc ctgaccagtt ccagcgcctg acgcattttg
      421 ctcgacgacg ggcagtcaga agaaccaaag ttggagtgcg acaacagagc aacgcgcggt
      481 tcaataccga aacgacggac agtttctgcc gccatcaagg tgatctccgc cagctcttct
      541 gcgtctggtt cgtcattgac gtaggtatcg gcaataaagg tgttaccact cggcagcagc
      601 agcgcgttca ttgcacctgc ggtgtgaacg ccatcgcgat aaccaaagac atttttcacc
      661 acgctaaaat gttcatgata atcacccacc gtaccgcaaa tcattgcatc ggcttcgcca
      721 cgctgaacca tgatcgcgcc gatcactgtc gggttactga tcagcgcccg ctgcgcctgt
      781 tcctgagtga cgccgcgacg cttcatgatc tggaagtatt cggtccagta ctctttaaag
      841 cgcggatcgg attcgttatt gacgatctca aaatcaacgc ccgctttgat ctgcaagccc
      901 agtttctgaa tgcgcatttc gatcacgttc ggacgaccga taaggatcgg tttcgccagt
      961 cccagcgtta ccagttcctg agtggcatgc agaacgcgcg cctcttcccc ttccggcaga
     1021 actacgcgct tcggcgcttt gcgagcctgg gagaaaatcg gcttcataaa caggttggtt
     1081 ttgtaaacga actcagtcag cttgtcgatg tagacgtcga aatcagcaat cggacgcgtc
     1141 gccacgcccg actccatcgc cgctttcgcg accgcaggag cgatcttaac gatcaagcgc
     1201 ggatcaaacg gttttggaat gatgtattcc ggaccaaagc tcagatcctg atcgccatac
     1261 gctgaagcca ccacttcgct ctgttccgca tgggccagtt ctgcaatcgc gcgtaccgcc
     1321 gccagtttca tctcttcgtt gatggcggtt gcgccaacgt ccagcgcgcc acggaagatg
     1381 aacgggaagc aaaggacgtt gttgacctgg tttggatagt cagaacgacc ggtgcagatg
     1441 atggcatccg gacgcacttc tttcgccagc ggcggcagaa tttccggttc cggattcgcc
     1501 agcgccagaa tcattggtgc ccgagccatt ttcttcacca tttcctgggt cagcactttc
     1561 gggccggaac agcccaggaa aatatccgca ccttcaatca catcatcgag ggtacgtttg
     1621 ccgtcatcca ccaccgcata tgcggctttg gtttccgcca tgtttggctc acggccttga
     1681 tagataacgc cttttgaatc gcaaaccacg atgttatgtt tttgcagacc cagcgctacc
     1741 agcagattca tacaggcgat tgctgcggca cccgcgccgg aaaccaccat ccgcacgtcg
     1801 gagatgtttt tctccaccac gcgcaagccg ttgaggatgg cggcagtgct gatgattgcc
     1861 gtgccgtgct gatcgtcgtg gaataccgga atattcatcc gctcgcgcag tttctgttca
     1921 atatagaaac attctggcgc tttaatgtct tcgaggttga ttccgccgaa ggtcggttcg
     1981 agcgcagcga caacttcaat aaatttgtcc gggtcgagtt catcaacttc aatgtcaaat
     2041 acatcaatcc cggcgaattt cttaaacaga acgcccttgc cttccatcac cggtttgcca
     2101 gccagcgcgc caatgttgcc taaccccagc accgccgtac cgttagagat caccgccacc
     2161 aggttaccgc gggcggtata tttgtaggct tttaacgggt ctttttcgat ttcaagacaa
     2221 ggtgcggcaa cgccgggtga gtaggccagc gccagatcgc gctgcgttgc cagaggcttg
     2281 gttggagaaa cctggatttt ccctggaact ggaaattcat ggaaatcaag tgcactttgt
     2341 tttaactggt catccatttg gttgttcctt tcacgtaacg ttcacaaata aagtgtgtgg
     2401 gcaacacccc ctgcccacaa cgtggcgcac attattacct tgccggagtc tacagacttt
     2461 gagcaagtcc aaactctcac cattaatata atgttttggt aataatccta taacactgat
     2521 gttacctgct taatccagca ataccatgcc tgtctgctat gcttttttga tgcgtttagc
     2581 gaaatttctc agaagtgtga attaacgcac tcatctaaca ctttactttt caaggagtat
     2641 ttcctatgaa cgagttagac ggcatcaaac agttcaccac tgtcgtggca gacagcggcg
     2701 atattgagtc cattcgccat tatcatcccc aggatgccac caccaatcct tcgctgttac
     2761 tcaaggctgc cggattatca caatatgagc atttaataga cgatgctatc gcctggggta
     2821 aaaaaaatgg caagacccag gaacaacagg tggtcgcagc gtgtgacaaa ctggcggtca
     2881 atttcggtgc tgaaatcctc aaaatcgtac ccggtcgcgt gtcaacagaa gttgatgcac
     2941 gcctctcttt tgataaagaa aagagtattg agaaggcgcg ccatctggtg gacttgtatc
     3001 agcaacaagg cgttgagaaa tcacgcattc tgatcaagct ggcttcgacc tgggaaggaa
     3061 ttcgcgcggc agaagagctg gaaaaagaag gtattaactg caacctgacg ctgctgtttt
     3121 cttttgcaca ggcacgggcc tgtgcggaag caggcgtttt tctgatttcg ccgtttgtcg
     3181 ggcgtattta tgactggtat caggcacgca agccgatgga cccgtatgtg gtggaagaag
     3241 atccgggcgt taaatcggtg cgcaatatct acgactacta taagcaacac cactatgaaa
     3301 ccattgtgat gggcgcgagc ttccgtcgca ccgaacaaat cctcgcctta accggctgcg
     3361 atcgactgac tatcgcaccg aatttgctga aggagctgca ggaaaaagtt tcgccagtgg
     3421 tacgtaaatt aatcccacct tctcagacgt tcccacgccc agctcccatg agcgaagcgg
     3481 agttccgttg ggagcacaat cagtatgcga tggcggtaga aaaactgtct gaaggcattc
     3541 gtctgttcgc cgttgatcaa cgcaaactgg aagatcttct tgccgccaaa ctataaacca
     3601 gccacggagt gttatatgtc ccgaaaagac cttgccaatg cgattcgcgc actcagtatg
     3661 gatgcggtac aaaaagccaa ttctggtcat cccggcgcgc cgatgggcat ggctgatatt
     3721 gccgaagtgc tgtggaacga ttttcttaaa cataacccta ccgacccaac ctggtatgat
     3781 cgcgaccgct ttattctttc caacggtcac gcgtcgatgc tgctctacag tttgctgcat
     3841 ctgaccggtt acgacctgcc gctggaagag ctgaagaact ttcgtcaact gcattcgaaa
     3901 accccaggcc acccggagat tggttatacg ccaggcgttg aaaccaccac cggtccactt
     3961 ggacaaggtt tggcgaacgc cgtcgggctg gcgatagcag agcgtacact ggcggcgcag
     4021 tttaaccagc cagaccatga gatcgtcgat cacttcacct atgtgtttat gggcgacggc
     4081 tgcctgatgg aaggtatttc ccacgaagtc tgttcgctgg caggcacgct gggactgggc
     4141 aagctgattg gtttttacga tcacaacggt atttccatcg acggtgaaac agaaggctgg
     4201 tttaccgacg atacggcaaa acgttttgaa gcctatcact ggcatgtgat ccatgaaatc
     4261 gacggtcacg atccgcaggc ggtgaaggaa gcgatccttg aagcgcaaag cgtgaaagat
     4321 aagccgtcgc tgattatctg tcgtacggtg attggctttg gatcgccgaa taaagcaggt
     4381 aaggaagagg cgcacggcgc accgctgggg gaagaagaag tggcgctggc acggcaaaaa
     4441 ctgggctggc accatccgcc atttgagatc cctaaagata tttatcacgc ctgggatgcc
     4501 cgcgaaaaag gcgaaaaagc gcagcagagg tggaatgaga agtttgccgc ctataaaaag
     4561 gctcatccgc aactggcaga agagtttacc cgtcggatga gcggtggttt accgaaggac
     4621 tgggagaaaa cgactcagaa gtatatcaat gagttgcagg cgaatccggc gaaaatcgcc
     4681 acccgtaagg cttcgcaaaa tacgcttaac gcttatggcc ccttgctacc ggagctgctc
     4741 ggcggttcgg cggatctggc tcccagcaac ctgaccatct ggaaaggttc tgtttcgctg
     4801 aaggaagatc cggcgggcaa ctacattcac tacggggtgc gtgaatttgg catgaccgct
     4861 atcgccaacg gcatcgcgca ccacggcggc tttgtgccgt ataccgcgac gttcctgatg
     4921 tttgttgaat acgcccgtaa cgccgcgcgg atggcggcac tgatgaaagc gcggcagatt
     4981 atggtttata cccacgactc aattggcctg ggcgaagatg gtccgacgca ccaggctgtt
     5041 gagcaactgg ccagcctgcg cttaacgcca aatttcagca cctggcgacc gtgcgatcag
     5101 gtggaagcgg cggtgggctg gaagctggcg gttgagcgcc acaacggacc gacggcactg
     5161 atcctctcaa ggcagaatct ggcccaggtg gaacgtacgc cggatcaggt taaagagatt
     5221 actcgtggcg gttatgtgct gaaagacagc ggcggtaagc cagatattat tctgattgcc
     5281 accggttcag agatggaaat caccctgcaa gctgcggaga aattagcagg agaaggtcgc
     5341 aatgttcgcg tggtttccct gccctcgacc gatattttcg acgcccagga tgaggaatat
     5401 cgggagtcgg tgttgccttc taacgttgcg gctcgcgtgg cggtggaagc aggtattgcc
     5461 gattactggt acaagtatgt tggtctgaaa ggggcaattg tcgggatgac gggttatggg
     5521 gaatctgctc cggcggataa gctgttcccg ttctttggct ttaccgccga gaatattgtg
     5581 gcaaaagcgc ataaggtgct gggagtgaaa ggtgcctgat agtgatcgcc ggatgctgat
     5641 tgccggatgc gacgctgacg cgtcttatcc ggcctacagc gtcttatccg gcctacatgt
     5701 ccccgccatt ttgtttaacg ggtgatccac aacgtgggcc aggcgtctgg cccatgccag
     5761 ttatcgcagg tgggttctgc ggcgtaacgc accaggcgaa aacgctgacc gtcaaagcgc
     5821 cagcgcgcct gaatgccaca atcgcttaat ccgcgccctt tcgctaaggt caccagttca
     5881 cgcgatttct catcaaatgt tgcgttcatc agttccagtt cattcgtctc ctgcccgttg
     5941 ttgaacggca aacgcaaccg aaccgggcgc gaggctagcg gctttttacg cgacacaatc
     6001 catgccaaat caatggtgtt ataggcccct gcctcacagc tgatcatcat cagcgcttta
     6061 tcatcagtca gcgcagtgac attcacctca cgacgtaacg gatcaagcga gcagcgcaga
     6121 ccattcatcc gccagtttcc ataatccagc aaatcgttgc gttcttcgag tgagagtggc
     6181 gtcggcgttg ggttaaccac cgcgacctct ttcagcgcag gcgcgggcgg tacgctgagc
     6241 ggcggttcgt cccctttctt gatccacgcg gtttcactgc caacgcgctt ttgctgagca
     6301 tcaataaaca acaacgccgc ttttaagcca ctcagagaaa tggtctgatt gccgtcgcgt
     6361 aaggtgatcg ccttcccttc ctgaatcatc tgcaaaaacg cggtgatggt tgccgtatca
     6421 tcggttacta ataaccatgg tgaaatccgc cacttgtcac cacttaacgc caacggctca
     6481 ccatctaaca gcagccgtgg cgctatctct ccttcagacg catccggcga cttcaacccg
     6541 ccgcgctcaa tacgtaaaac ggcatcggta tgcgccccgg cgctgcggct cagggtcatc
     6601 accagtccat tatgatcgcc cgtattgcgc gccacgcaga agttttggtt attacaggtg
     6661 acctgccagt cggaaaacgc ccgttgcgct agtgccgccc acaccaaaga cgtcggcaac
     6721 aaagcgaaaa aaaagagaag gaaaatgcga tagcgcatgg acggtacgac cccagaagtt
     6781 caaacaaaca gcacaagtcg tatcttcgtt gcgcaggtag cgcggctcaa tcagatttgt
     6841 cggattgagc caataataaa caacgaaata ttattgttat ttttcagtcc attaaatgtg
     6901 acgtttgcaa atagttaagc aataacaccg tcttaccgtc acgtatctcg ccggttttga
     6961 tcatctccag cgcctggctg aacggcagct cgagcacttc aatatcttca tcttcaacac
     7021 cgccaccggc gttagcgcgc tgattgtcac tgtattcggc gataaaaaag tggattagct
     7081 cagtcacacc gccaggcgac atatacagtt caaataattt gcgcacttcg cccacttcat
     7141 agcccgtctc ttcaatcgct tctttacgaa tgcacacttc cggttcgtcg ttatccagca
     7201 gcccggcgca ggtttcaatc agctgcccgc tttcattgcc attaacccag gtagcgacac
     7261 ggaactgacg aatcagaacc acggtctttt tcttcgcgtt gtacaggagg atcgtcgcac
     7321 cattaccgcg atcgtaaact tcacgtttat ggcggataac ttagccatct ttgcgcgtga
     7381 gatcgtaagt aatgttgtgc agggtgaaat agttatcgga gagaattttg tctttaatga
     7441 gggtgatttg ttgcgtcata ccgactccac agcgcgaaat gaacaattat attacgctgt
     7501 gaagtcgggt ttgtctgcgc aggctatcag tgagatttga ctgattttac ccccagccag
     7561 tcaataatcc cctgtgccgc atgacgtcct tctgccattg cggtaaccac cagatccgca
     7621 ccacgcacgg cgtcgccacc ggcgaagatt ttcggattgg tggtctggta acggtactgg
     7681 ctttccacat ccgcgatgat gcggccccat ttgtctaccg ttacaccgtg cgactccagc
     7741 cacggcatcc cgtgtggatt gaagccaaac gccataatca ccgcatcggc tggcatgaca
     7801 aattcactgc cctcaatcgg tacaagtcgc cggcgcccct gggcatccgg ctcgccaaga
     7861 cgcgtgcgca ggaaacgaat tccgcaaacg tgaccttgct cattctgctc aagcgccacc
     7921 ggctggacgt taaattcgaa gttggccccc tcttcgcggg cgttcttcac ctctttcttc
     7981 gagcctggca tgttagcttc atcacgacga taagcgcagg tgacgttact cgcgccgtgg
     8041 cgcagcgcgg tacgcacaca gtccatcgcg gtgtcgccgc cgcccagtac cacgacgttg
     8101 agtccggcgg tattgataaa cggctcttcc ggtagctctt cgagtcccat cacctgttta
     8161 gtgttggcga tgaggaacgg cagcgcgtca taaacgcccg gcgcatcttc attgggtaaa
     8221 cccgctttca tggaacggta agtgcctacg ccaacgaaga ccgcgtcgta ttgttccaaa
     8281 agcgaatcca aagagacatc tttacccact tcacaattga gttcgaagtg aatccccatc
     8341 gcgctgaaga tttcccgacg gcgtgccagc agggatttat ccagtttgaa agaaggaatg
     8401 ccgaaagtga gcaagccacc aatttctgga tggcgatcgt acaccgtcac cgccacgcca
     8461 ttgcgggtca gaacatccgc acaggccagc cctgccggac ccgcaccgat aatcgccacc
     8521 cgcttgtcca ctttggtgac atggcttaag tcaggacgcc aacctttcgc caacgcctga
     8581 tctgaaatgt agcgttcaat gttgccgata gttaccgcgc cgtgctcatc gcgaatagta
     8641 caggcacctt cacacaaacg gtcctgcgga caaacgcgtc cggtaatttc cggcagggtg
     8701 ttggtcaggt gagaaagctc gacggcggcg tcgatgtttc cggctttcac cagttcaatc
     8761 cactgcggaa tatggttatg cagcgggcag gtccattcac aaacgctgtg ctcgccgcat
     8821 ttaaggcagc gcgaggcttc ccgttgtgcc tgatcggcac ggaatggcag ataaatttca
     8881 tcaaaaccgg ttttgcgcgc ttcaatcgcc agtttatccg gctcgccacg cgcgggcgtt
     8941 gcctgcattt gttcgacttt actcattacc ggcatttctt gtgcagcggt actggcatgc
     9001 cacggttgat gctcctgacg tgcggtgcgc aagcggcggg atttcgccat gccggagagt
     9061 gcgacgtcag tgaccagttg cagcgcgtcc gccgggcaat tctcgacaca ggcaggaccg
     9121 ttttcgcgcc ctgcacaaag gtcgcattta tgcgccgtgg cttttacttt tcctgccgcg
     9181 acgggtgtca ggacgatttg catcgtacca aacggacagg ccaccacgca ggatttacag
     9241 ccaatacact tttgctgatt gacctgaatg ctgtcatcaa cgtggctgat tgcgccatta
     9301 gggcagctac gggcgcaggg cgcatcttca caatggtgac aggtcactgc actacgttgc
     9361 tgctgatgtt tgataaccgt aattcgggga tgaaaatggt gttggctcag gacatgttgc
     9421 tcatcattgt gagccatgac acaggcgatt tcacaagcat gacaacccag acattgctga
     9481 ctgttggcca taataaaacg attcataacg accttctttt ttggttgtaa aaaccttatt
     9541 ctttatatga gtgttgttat tacccgactt acaggggaat cggcaatgtt catgtgccca
     9601 gaataagtaa ctatttcgct ataaactgtg gcagatcaaa taatcccatc actgactaaa
     9661 ttgcgtttta gcgaactgga acattaatga ttttttgtgg agaagacgcg tgtgattgtt
     9721 aaacgacccg tctcggccag tctggcccgg gccttttttt acattgtgct gctgtcgatt
     9781 ctttccacgg gtatcgctct gctaactctg gcgagcagtt tgcgcgacgc tgaggctatc
     9841 aatattgccg gatcgctgcg tatgcagagt taccgcctgg gctacgactt gcaaagtggc
     9901 agtccacaac tcaatgcaca tcgccagttg tttcagcagg cactgcattc atcggtatta
     9961 accaatctca acgtctggta tgtgccagaa gcagtaaaaa ctcgctatgc gcatctgaat
    10021 gccaactggc tggagatgaa taatcggctc agcaagggcg atttgccgtg gtatcaggcc
    10081 aatattaata attatgttaa tcagatagac ctgttcgtgc tggctttaca gcactacgct
    10141 gaacgcaaaa tgctgctggt ggtggcgatt tccctggctg gcggtatcgg tattttcacg
    10201 ctggtctttt ttactttgcg ccgtatacgc catcaggtgg ttgccccgct gaatcagctg
    10261 gttaccgcca gtcaacgtat tgaacacggg cagttcgact cgccgccgct ggataccagt
    10321 ctgccgaatg agcttggtct gcttgcaaaa acctttaacc agatgtcgag cgagctgcat
    10381 aaattgtacc tttcgctgga ggcgtcagta gaagaaaaga cccgcgatct ccacgaggcc
    10441 aagcgtcgtc tggaggtgtt gtatcaatgt tcgcaggcgc tgaacacaag ccagattgat
    10501 gtgcattgtt tccgccatat tttgcagatt gttcgcgaca atgaagcggc tgaatatctg
    10561 gagttaaatg tcggtgaaaa ctggcggatt agcgaagggc aaccaaaccc ggaattgccg
    10621 atgcagattt taccggtgac aatgcaagag acggtttacg gcgaactgca ctggcaaaat
    10681 agtcacgttt catcatcaga accgctgctt aacagcgttt cgtcgatgct gggacgcggt
    10741 ttgtacttta atcaggcgca aaagcatttt cagcagttat tgttgatgga agaacgtgca
    10801 accatcgccc gcgaattgca cgactcgctg gctcaggtac tttcttactt acgtatccag
    10861 ttgacgttac tgaagcgttc gataccggaa gataacgcca ccgcacaaag tatcatggcc
    10921 gatttttccc aggcgttgaa tgatgcttat cggcagttac gcgagctgtt gactactttc
    10981 cgcctgacgc tgcagcaggc ggatctcccc tccgcgttga gggaaatgct ggatacgtta
    11041 caaaatcaaa ccagcgccaa actgaccctc gactgccgtc tgccaaccct ggcgctggat
    11101 gcgcaaatgc aggtgcattt gttgcaaatt attcgcgaag cggtgctgaa tgcgatgaag
    11161 cacgccaacg ccagcgaaat cgccgtcagt tgcgtcaccg cgccggacgg caatcacacg
    11221 gtttatatcc gtgataacgg gattggtatc ggtgaaccga aagaacccga aggtcattat
    11281 ggtctgaata tcatgcgcga acgcgcggaa cggctgggtg ggacgctgac tttttcacaa
    11341 ccttccggcg gcggcacgtt agtgagtatt agctttcgct ctgcggaggg tgaggaaagt
    11401 cagttgatgt aatgcctcct actgaccaaa gaatacttgc acttaaggtt cagtataaaa
    11461 gggcatgata atttacatta actccttttt ttctccacga ttggctcgta ccttgccgct
    11521 acagtgaagc aagtcaagcc tacaacgata cgcagaaaca cgaggtcctc ttttaatggc
    11581 gaatttcttt attgatcgcc ccatttttgc ctgggtgctg gcaatcctgt tgtgtctgac
    11641 agggaccctg gcgatttttt cattgcccgt tgaacaatac cccgatctcg cgccaccgaa
    11701 tgtgcgagtg accgctaact atcccggcgc atcggcccag acgctggaaa acaccgtgac
    11761 ccaggttatc gagcaaaata tgaccggcct cgataatctc atgtatatgt catctcagag
    11821 cagtggcatc ggtcaggcat ctgtcacttt aagttttaaa gcaggcaccg atccggacga
    11881 agccgtgcag caagtacaaa accagctgca atcagccatg cgaaagttac cgcaggcggt
    11941 gcaaaatcag ggcgtgacgg tgcgtaaaac cggcgatacc aatattctga ccattgcttt
    12001 cgtctctacc gatggttcga tggataaaca ggatattgcc gattatgttg ccagtaatat
    12061 tcaggacccg ttaagccgcg tgaatggcgt cggggatatc gatgcctatg gttcgcaata
    12121 ttccatgcgt atctggctgg acccggcgaa actcaacagt ttccagatga cggctaaaga
    12181 tgtcactgat gccattgagt cacagaacgc gcagattgcg gttgggcaac ttggtggtac
    12241 accttccgtc gataagcagg cgctcaacgc caccattaac gcccagtcac tgctgcaaac
    12301 accagaacag ttccgcgata tcaccttgcg ggtgaatcaa gacggctcag aggtaaggct
    12361 gggcgatgtc gccaccgtcg aaatgggggc ggagaaatac gattatctta gccgcttcaa
    12421 tggtaagcca gcctccgggc tgggggtaaa actggcctcc ggcgcaaacg aaatggcgac
    12481 agcggagctg gtgctcaatc gtctcgacga gctggcgcag tacttcccgc acggactgga
    12541 atacaaggtg gcgtatgaaa ccacctcgtt tgttaaagcc tccattgaag acgtggtgaa
    12601 aacgctgctg gaagctatcg ccctggtttt cctcgttatg tatctgttcc tgcaaaactt
    12661 ccgcgccacg ctgataccca ctatcgccgt gccggtggtg ttgatgggaa ccttctccgt
    12721 actttacgcc ttcggttaca gcgtcaacac cttaaccatg ttcgcgatgg tactggcgat
    12781 cggtctgctg gtggatgacg caatcgtggt ggtggaaaac gtcgaacgta ttatgagtga
    12841 ggaaggactc actcctcgcg aagccacacg taaatcgatg gggcagatcc agggggcact
    12901 ggtcgggatt gcgatggtgc tttcggcggt atttgtacca atggccttct tcggcggcac
    12961 caccggtgcc atctatcgcc agttctctat taccattgtt gcggcgatgg tgctgtcagt
    13021 actggtagcg atgatcctca ctccggctct gtgtgccaca ctacttaagc cactgaaaaa
    13081 aggtgagcat cacgggcaaa aaggcttttt tgcctggttt aaccagatgt ttaaccgcaa
    13141 cgccgaacgc tacgaaaaag gggtggcgaa aattctccac cgcagcctgc gctggattgt
    13201 gatttatgtc ctgctgcttg gcggcatggt gttcctgttc ctgcgtttgc cgacgtcgtt
    13261 cttaccgctg gaagaccgtg gcatgtttac tacctcggta caattgccca gcggttcaac
    13321 ccaacaacag accctgaaag tcgttgagca aatcgagaaa tattacttca cccatgaaaa
    13381 agacaacatc atgtcggtgt ttgccaccgt tggttctggc cctgggggta acgggcaaaa
    13441 cgtggcgcga atgtttatcc gcctgaaaga ctggagcgaa cgcgacagta agaccggcac
    13501 ctcgtttgcc attatcgagc gtgcaacaaa ggcgtttaac aagattaaag aagctcgcgt
    13561 tatcgccagc agcccgccag caattagcgg tcttggtagt tctgcgggtt ttgatatgga
    13621 gttgcaggac cacgctggag caggtcacga tgcgctgatg gcagcacgta accagttgct
    13681 ggcgctggcg gcggaaaacc cggagctaac ccgtgtgcgc cataacggcc tcgacgacag
    13741 tccgcagttg cagattgata tcgaccagcg taaagctcag gcgctgggcg ttgctatcga
    13801 cgatattaac gacacactgc aaaccgcctg gggttcgagc tatgtgaatg actttatgga
    13861 tcgcggtcgc gtgaagaaag tctatgtgca ggcagctgcg ccgtatcgca tgctgccaga
    13921 tgacatcaat ctctggtatg tccgaaataa agatggcggc atggtgccct tctctgcttt
    13981 cgcgacctca cgctgggaaa caggctcgcc gcgtctggaa cgctataacg gttattctgc
    14041 ggttgagatt gttggggaag ccgcaccggg ggtcagtacc ggtacggcga tggatattat
    14101 ggaatcgtta gtgaagcagc tgccaaacgg ctttggtctg gagtggacgg cgatgtcgta
    14161 tcaggagcga ctttccggcg cgcaggctcc ggcgctgtac gccatttcct tgctggtggt
    14221 attcctgtgt ctggctgcgt tgtatgaaag ctggtcggtg ccgttctcgg taatgctggt
    14281 cgtgccgctg ggggtaatcg gcgcgctgct ggcaacctgg atgcgcgggc tggaaaacga
    14341 cgtttacttc caggtgggcc tgttaacggt cattggttta tcggcgaaaa acgccatcct
    14401 gatcgtcgag tttgctaacg agatgaacca aaaaggccac gacctgtttg aagcgacgct
    14461 ccacgcctgc cgtcagcgtt tacgcccgat tctgatgacc tcgctggcat ttatcttcgg
    14521 cgtattgcca atggcaacca gcacgggtgc cggttccggt ggtcagcatg cggtgggtac
    14581 tggcgtaatg ggcgggatga tttcagccac tattctggct atttacttcg tgccgctgtt
    14641 ctttgtgctg gtgcgccgcc gcttcccgct aaagccgcgc ccggaataag caataaaaaa
    14701 ggcgacatgc caatgtgtcg cctttttcaa ctttccgatt aagaacctgc tcagcgggtt
    14761 cttgctgttt atactttgtc tcaggaatta cttacgaagc ataacttcga taaagtcttt
    14821 ccagttcccc agttcacgtt caatcataac aacctctctt ataattatgg gtattctacg
    14881 gaaacaatat accgtggtga agctaattta ctcgattgct gcgatgacta cctccggggg
    14941 gacaaatctt atgtaaatac tatggtcctg cggtgatgat ttgtatgtga tacacagcaa
    15001 catttcgaga tattcatacg gcatctaata ctgatttaat tcaggtta
//
LOCUS       DAZRUF010000091        14982 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZRUF010000091 DAZRUF010000000
VERSION     DAZRUF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14982
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.091"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..161
                     /locus_tag="ACHXOL_002218"
     CDS             <1..161
                     /locus_tag="ACHXOL_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR6809190.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="ACHXOL_002219"
     CDS             complement(199..1260)
                     /locus_tag="ACHXOL_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR6809191.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="ACHXOL_002220"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="ACHXOL_002220"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR6809192.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLLERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="ACHXOL_002221"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="ACHXOL_002221"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR6809193.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4155)
                     /locus_tag="ACHXOL_002222"
     CDS             complement(3454..4155)
                     /locus_tag="ACHXOL_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR6809194.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHXOL_002223"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHXOL_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR6809195.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHXOL_002224"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHXOL_002224"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR6809196.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6576..7613
                     /gene="purM"
                     /locus_tag="ACHXOL_002225"
     CDS             6576..7613
                     /gene="purM"
                     /locus_tag="ACHXOL_002225"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR6809197.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7613..8251
                     /gene="purN"
                     /locus_tag="ACHXOL_002226"
     CDS             7613..8251
                     /gene="purN"
                     /locus_tag="ACHXOL_002226"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR6809198.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHXOL_002227"
     CDS             8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHXOL_002227"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR6809199.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10493..12034
                     /gene="ppx"
                     /locus_tag="ACHXOL_002228"
     CDS             10493..12034
                     /gene="ppx"
                     /locus_tag="ACHXOL_002228"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR6809200.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHXOL_002229"
     CDS             complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHXOL_002229"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR6809201.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14498..14650)
                     /locus_tag="ACHXOL_002230"
     CDS             complement(14498..14650)
                     /locus_tag="ACHXOL_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809202.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14668..14859
                     /locus_tag="ACHXOL_002231"
     CDS             14668..14859
                     /locus_tag="ACHXOL_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR6809203.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT         3962 a         3819 c         3692 g         3509 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggta agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc tatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatagc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actaacagat taccgtccag cgcctgaata atcagataca ctgcgaagca
      421 gctccagaat tccgtgcctg caccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccaa gtggcgatcc ccaccacgat
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta atgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gatcaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacaa tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagccagca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgctggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaaa aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgccccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aatcgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgca gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctaatcagtt
     4321 tgaagatcaa acttaagccg ataccgacga tggtcgccag cgccatacct ttcagctccg
     4381 ccgcgccaat gtttaccttc gcgccactga caccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt ttgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccac
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtagagcca aagaagccgg agatcacagt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac ggaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag ctaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcatcccca gaccggcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaaatcga cggattatac
     6301 ctcctttctt caaggcggca atattctttt cgttgacttt agtcaaaatg ataacggttt
     6361 gagataaagt tattttatat tcaaatggtt atgaaagaag attattccat ccgaaaacta
     6421 acctttgccc tggcacaagt cttctttcgc cgcgcgtctg gggaaaagac gtgcaaaaag
     6481 gttgtgcaaa gcagtctcga aaacgtttgc tttccctgtt agaattgcgc cgaattttat
     6541 ttttctaccg caagtaacgc gtggggaccc aagcagtgac cgataaaacc tctcttagct
     6601 acaaagatgc cggtgttgat attgacgcgg gtaatgctct ggttggaaga atcaaaggcg
     6661 tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt
     6721 gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc cggcactgac ggcgtaggta
     6781 ccaagctgcg tctggcaatg gacttaaaac gtcacgacac cattggtatt gatctggtcg
     6841 ccatgtgcgt taatgacctg gtggtgcaag gtgcagaacc gctgtttttc ctcgactatt
     6901 acgcaaccgg aaaactggat gttgataccg cttcagcggt gatcagcggt atcgcggaag
     6961 gatgtctgca atcgggctgt tcactggtgg gtggcgaaac ggcagaaatg ccggggatgt
     7021 atcacggtga ggattacgat gtcgcgggtt tctgcgttgg cgtggtagaa aaatcagaaa
     7081 tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat tgcactcggt tccagcggtc
     7141 cgcactcgaa cggctattcg ctggtgcgca aaattcttga agtcagcggt tgtgatccgc
     7201 aaaccaccga acttgatggt aagccattag ccgatcatct gctggcaccg acccgcattt
     7261 acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga
     7321 ccggcggcgg cttctgggaa aacattccgc gcgtattgcc agataatact caggcagtga
     7381 ttgatgaatc ttcctggcag tggccggaag tgttcaactg gctgcaaacg gctggtaacg
     7441 ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt cgggatgatt atcgccctgc
     7501 ctgctccgga agtggacaaa gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga
     7561 aaatcggtat catcaaagcc tctgattccg aacaacgcgt ggttatcgaa taatgaatat
     7621 tgtggtgctt atttccggca acggaagtaa tttacaggca attattgacg cctgtaaaac
     7681 caacaaaatt aaaggcacca tacgggcagt tttcagcaat aaggccgacg cgttcggcct
     7741 tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc atcgccagcg cgtttgacag
     7801 tcgtgaagcc tatgaccggg agttgattca tgaaatcgac atgtacgcac ccgatgtggt
     7861 cgtgctggct ggttttatgc gcattctcag cccggcgttt gtctcccact atgccgggcg
     7921 tttgctgaac attcaccctt ctctgctgcc gaaatatccc ggattacaca cccatcgtca
     7981 ggcgctggaa aatggtgatg aagagcacgg tacatcggtg catttcgtca ccgatgaact
     8041 ggacggtggc ccggttattt tacaggcgaa agtcccggta tttgctggtg atacggaaga
     8101 tgacatcacc gcccgcgtgc aaacccagga acacgctatt tatccactgg tgattagctg
     8161 gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg tggctggatg gtcaacgtct
     8221 gccgccgcag ggctacgctg ccgacgagta atgccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttt ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc
     8341 tggcatagtt ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa
     8401 taaaacggag taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt
     8461 tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt
     8521 gaaaggatgc gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc
     8581 ttcgctgaac tgaagcgacg catcattatt agcgaagaac aaggctccaa ctctcattcc
     8641 cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc
     8701 ctctacaacg agctgctgct ggagatggcg cgtaaccaga tcttcctgat taatgaacgc
     8761 cagctctccg tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag
     8821 cacattacgc cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat
     8881 tacacctatc tggcggtgga aattatccgt ggcgatacca tccgttacgc gctgctggag
     8941 atcccatcag ataaagtgcc gcgctttgtg aatttaccgc cagaagcacc gcgtcgacgc
     9001 aagccgatga ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc
     9061 ttctttgatt atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac
     9121 gatttagtgc atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag
     9181 cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt
     9241 gaagtgttac gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt
     9301 tatcataatt ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac
     9361 aaaccactgc cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt
     9421 gatgccattc gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg
     9481 ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt
     9541 taccgtgtgg caaaagattc acgtatcatc gactcgatga tccacgctgc gcacaacggc
     9601 aagaaagtga ccgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac
     9661 tgggcgaagc gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa
     9721 attcacgcca aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca
     9781 cacatcggga ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg
     9841 ctgaccgcag atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac
     9901 ccataccgcc cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg
     9961 ttatatgaaa tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc
    10021 accctgaagc taaataacct tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc
    10081 agctccggcg taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg
    10141 gaaggcatta gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac
    10201 cgggtttata tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg
    10261 atgacgcgca atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg
    10321 aagcagcggg tgctggacat catcgacata ttgttcagcg atacggtcaa agctcgttat
    10381 atcgataaag aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg
    10441 cagttggcga tttatgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat
    10501 acacgataaa tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt
    10561 tcacatggtc atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca
    10621 gcgggtgcat ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac
    10681 gcgcggtttg aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag
    10741 cgtctgtata gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa
    10801 acgcgcggaa aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg
    10861 tctgattttt atgggcgtgg aacataccca accggagaaa ggtcgcaaac tggttattga
    10921 tattggcggc ggatctacgg aactggtgat tggtgaaaat ttcgaaccga ttctcgttga
    10981 aagccgccgg atgggttgtg tcagctttgc ccagctttac ttccctggcg gggtcatcaa
    11041 taaagagaat tttcagcgcg ctcgtatggc ggcagcacaa aaactggaaa ctttaacctg
    11101 gcaattccgt attcagggct ggaacgtggc aatgggcgct tccggtacca taaaagccgc
    11161 ccatgaagtg ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga
    11221 aaaactggta aaagaagttt tacgtcaccg taatttcgca tcgctgagtt taccgggtct
    11281 ttccgaagag cggaaaacag tcttcgttcc tggactggcg attttatgcg gtgtgtttga
    11341 tgctttagcc atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta
    11401 cgaaatggaa ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc
    11461 caaccagtat cacatcgaca gcgaacaggc ccgacgggtg ctggatacca ctatgcaaat
    11521 gtacgaacag tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact
    11581 gcgatgggcc gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg
    11641 ccactccgct tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct
    11701 gatgatggcg acactggtgc gctatcaccg taaagcgatt aaactcgacg atctgccgcg
    11761 ctttaccttg tttaagaaga aacagttcct gccactgatt cagctattgc gccttggcgt
    11821 attactcaac aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga
    11881 tgacagtcac tggacactgc gtttcccgca tgactggttt agtcagaatg cgttggtact
    11941 gcttgatctg gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat
    12001 tgaagaagaa agtacacctg aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc
    12061 ggcccgcatt attcaggcac tttcgcgaat gggttcgatt tcattcaacg tatcaattaa
    12121 cggctgcggc ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc
    12181 ctcgcggatc tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc
    12241 cagatggcaa atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat
    12301 aaagctgccg tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc
    12361 gtagccggtg ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa
    12421 ggtgatttgc gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag
    12481 ttgccacgct tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc
    12541 ctgacatacc gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct
    12601 gttttcagcc ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa
    12661 accaaattcg tgcgcgaccg gcaagaagct atcggggacg atcagttcat cattctcacc
    12721 tttcatgcgc agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc
    12781 catcaggaaa aaatggttgt gttccagcgc ctgctgtagt cgattcatca tcgcgacttt
    12841 atctttcaat tcgcgttgca aatacattgc cccgcgacgc tgcatatttt ccggggcgtt
    12901 ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt
    12961 cactggcgag cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc
    13021 ccaaaagaaa cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga
    13081 ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg
    13141 ttccagcaag ggtgacagcc agtgagaaag tttttgcttg tattgaatcc gcagcatgat
    13201 gccatagttc ttaaccagca tttccatgcc agggatgcgt aaataacaaa gcgcagacca
    13261 gggagcatca cgcaacgcgc gattcagggc gcgaacattt ggcagatgaa ccaccgggtc
    13321 catatacgca agccgctgaa tgcgtctgac taccgctctc tgacgggttg ccagtacagc
    13381 catataattg acaataaaag agaataccag ataactggag gaggttatgg tcagctgcgt
    13441 ggtatagcca ggataaatgg gaatgtaatt ttgatagctg tggatgctga tcatcaacac
    13501 gaccgcccag agcagcgaaa tcagcttata accatagcgc atcgctcccc acatcatcag
    13561 gggcagcaat aatgacaagg tataattggt gctaaaaatt gtgctttttt catttaacgg
    13621 catgcataac agcaacaata aagcacctaa tgccagtagc cagagcgcga actctttttt
    13681 ggtgactttg gcatcaacct gctgttttaa ttgcgaataa tagctacgta aataaaatgg
    13741 atttcgcact acccgaatga tgaagtagca cagcgggaca ccgatcagat tacccaccag
    13801 taaggcctga taattgatta aagtcccgag gttaaaaggc atgacaccga ccagattttc
    13861 tctgctcgcc agtaatccta caaatgcagc aaactggaaa agtatcagaa acagcgttgc
    13921 aggaaacaca atctgccaga atatacgctg ggaaattaaa cgggtatcac catgtgaaac
    13981 gttgttacgc cggggagtaa agaccctgta accgccccaa cagagaatga taatgataat
    14041 aaaattagcc gttaatgata gcgtttcgta aaaacccagc tctgcatatt tgcggacaaa
    14101 aatccctaag gcgatccctg gcaatgccgc ccagctgaaa aacatcatca tgctgatcat
    14161 cagtgccaga ggcagataaa agagaaaaac ctcaccggaa gaaatatgcg cgaaagtatt
    14221 aatgtgggcg aaaatgggca agattaaaga aggcaggaac agcggaagcc cccaccattt
    14281 atcacgcatt tttatataag ttgcattcag tttcatagat gctcagcaga atcccccaca
    14341 tcctgaagga ggtgtattca gacaggcatc ccacctgact tcgaatgatg attattcatc
    14401 actatagaga gcattgattc taagtgtcat atgaaagttc caattgatat atatcaaaca
    14461 aaataaccct gattaatgaa ttattacgtt tatcatgtta attcatcatt attacatcat
    14521 cattgtaaat aattaaatta acttccataa cattaaaata tgtatccact gacgtttttt
    14581 tacataacga agaattgacc attttgtcct gttgtgcctt aatgtaagta ccgtccacag
    14641 cgtgggacat acttcaagga accttttgtg agtcaggcaa ccagtatgcg aaaacgacac
    14701 cgatttaaca gtcgcatgac ccgtatcgta ctgctcatca gctttatctt cttctttggc
    14761 cgttttatct actcgtccgt cggtgcctgg cagcaccatc agagcaaaaa agaagctcag
    14821 caatccacac tctccgtcga atcaccggta caacgttagc ggttaccttc ttcactttca
    14881 cagaacataa cggcacttcg ctgtcggatg cttttgctct ttgggattat caaagcggca
    14941 gatattcttt catcttaaat tttacgtctt tgtcctgact ga
//
LOCUS       DAZRUF010000092        14886 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZRUF010000092 DAZRUF010000000
VERSION     DAZRUF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.092"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            261..1559
                     /gene="kgtP"
                     /locus_tag="ACHXOL_002232"
     CDS             261..1559
                     /gene="kgtP"
                     /locus_tag="ACHXOL_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR6809204.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1556..1879)
                     /locus_tag="ACHXOL_002233"
     CDS             complement(1556..1879)
                     /locus_tag="ACHXOL_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR6809205.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHXOL_002234"
     CDS             complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHXOL_002234"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR6809206.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHXOL_002235"
     CDS             complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHXOL_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR6809207.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHXOL_002236"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHXOL_002236"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR6809208.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHXOL_002237"
     CDS             complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHXOL_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR6809209.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7479..8516
                     /locus_tag="ACHXOL_002238"
     CDS             7479..8516
                     /locus_tag="ACHXOL_002238"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR6809210.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSLAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHXOL_002239"
     CDS             complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHXOL_002239"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR6809211.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9558..9941
                     /gene="grcA"
                     /locus_tag="ACHXOL_002240"
     CDS             9558..9941
                     /gene="grcA"
                     /locus_tag="ACHXOL_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR6809212.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHXOL_002241"
     CDS             complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHXOL_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR6809213.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10687..11568
                     /locus_tag="ACHXOL_002242"
     CDS             10687..11568
                     /locus_tag="ACHXOL_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR6809214.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHXOL_002243"
     CDS             complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHXOL_002243"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR6809215.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13243..13980
                     /gene="trmN"
                     /locus_tag="ACHXOL_002244"
     CDS             13243..13980
                     /gene="trmN"
                     /locus_tag="ACHXOL_002244"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR6809216.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13965..>14886)
                     /locus_tag="ACHXOL_002245"
     CDS             complement(13965..>14886)
                     /locus_tag="ACHXOL_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR6809217.1"
                     /translation="VANLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFM
                     PDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLG
                     IDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLE
                     CLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVG
                     IVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKES
                     RGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3588 a         3767 c         3899 g         3632 t
ORIGIN      
        1 gatgcgacac tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggttt
       61 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taagctcctc
      121 aataaccatt ttcctgctaa ctaaattcat ggttaaggtt gcacaatgat atgcaacaaa
      181 tgtataacat ttcctttacc aaaaaaaata aacaaaagcg accgacaaaa gcatcggatt
      241 acggcaggag acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa
      301 gtagtgatac tcgtcgccgc atttgggcaa ttgtgggggc ctcttcaggt aatctggtcg
      361 agtggttcga tttctatgtc tactcgttct gttcactcta cttcgcccac atcttcttcc
      421 cttccgggaa cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc
      481 tgatgcgccc aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa
      541 aatcgatgct gctatcggtg tgtatgatgt gtttcggctc actggtaatc gcctgtttgc
      601 ctggttatga aacaatcggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc
      661 agggattatc cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg
      721 ttgaagggcg caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac
      781 tgctagccct actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag
      841 agtggggatg gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt
      901 tacgtcgtca gttagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat
      961 ctctgaaagg attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg
     1021 cgggctccct ttgtttctat accttcacta cttatatgca gaagtatctg gtaaatactg
     1081 cgggaatgca tgtcaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc
     1141 ttattcaacc actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat
     1201 gtttcggttc gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt
     1261 cctcgcctta tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata
     1321 catcaatcag tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg
     1381 ttggcctgtc atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt
     1441 tgtcgctgaa atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg
     1501 tggtggcgtt tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt
     1561 gacgggtcag ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat
     1621 ccttccagct ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca
     1681 aactgacaga gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct
     1741 gtgaatattc atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat
     1801 cctgcccgct ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac
     1861 agacaaaaag aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt
     1921 gtgattacag gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga
     1981 gtttacgaac cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga
     2041 tggtggtatg ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agcttcagtt
     2101 gtggatcgtg gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt
     2161 tattaccggt gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt
     2221 tgtcgtcatc tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac
     2281 ggctcaggaa acgacgcaga ttgatctcat agagataagg caatgcgcca attatcttga
     2341 aaggttcatc ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga
     2401 cctttttccc ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga
     2461 agtatggcgt acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct
     2521 tgttcaacag actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt
     2581 cggcatcgcc ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat
     2641 cgttcttgat ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc
     2701 cattcataat attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga
     2761 taagatgata acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc
     2821 tggcaccgct ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa
     2881 gggcttcacg ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct
     2941 gcgccatgcg gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac
     3001 gttgtgcacg atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag
     3061 cttcatacaa cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga
     3121 caatgcaaat gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg
     3181 cgggagcgta aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa
     3241 ggtgttgttg atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg
     3301 ttcattgaag ggtcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc
     3361 ccgattaagt ggtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt
     3421 tagcgtaagc ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg
     3481 ggctagcgcc accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc
     3541 gtgatctcgc gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt
     3601 gagatccgag cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc
     3661 acgcgtgacg ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat
     3721 ttcccgatcg tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat
     3781 ctcgctaaag tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag
     3841 ttgtggctca tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc
     3901 tacccattct tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc
     3961 ttcaaacggc gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag
     4021 caaaggatga atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg
     4081 caccagaagc tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat
     4141 ctttttactt ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg
     4201 cagtgcgacg acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat
     4261 caacggcccg aaaaccggat cgtgctcaac cacaacccgc aactcctgag cgccagcacg
     4321 gttagccata ctttgcacca acaggccgtg gacccgcgcc tgtggccagg ccatttttac
     4381 gcgatcgaaa atagcgttcg ccgcttgctg gacttcattg gctgtacgca ggtaaagcat
     4441 gacgccctga acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg
     4501 ataaccaatc tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg
     4561 gagcgtgttc atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt
     4621 agccccttcg gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag
     4681 attgctgggc aacgtcggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat
     4741 atgcataaaa gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct
     4801 gaataaacgt cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac
     4861 atatttgctg cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc
     4921 gggagcagcg gcgctgggcg aatgaataac catcagcgca tcaaaatcct ggctgtggag
     4981 cagaatgtcc agcgttttga cataatgctc gctgctggcg tcatcgcgca gatcgagagg
     5041 gttagagact gccacatgtt ctggcaatgc atcgcgcaat ttctggcagg tttcttcgct
     5101 tagcgttgcc agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc
     5161 agcaccgttg ctgataatca tcaaccggtc gccacgcagc gggcgcatat ggctaagggt
     5221 ttccaccgcc gaaaacagtt cgtgggtatc ctgtacccgc aacaaaccgg cacgctgaat
     5281 agccgcatcc catgccgggt ccattcctgc cgtcgtgttg agcagtcgct gtgccgccgg
     5341 gctacgtccg cttttaatca ccagaatcgg tttattacgc gaggcactac gggctgccga
     5401 aacaaagcgt cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact
     5461 gtcgcgcgcc agatagtcga gcaattcatc aacgtcgata tccaggctgt cgccgagcgc
     5521 aataaagtag gaaaagccca tctcacgctg ttgcgcccag tcgaggatgg tgttggagac
     5581 ggctgccgat tgtgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct
     5641 ggcattcaga ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat
     5701 gttatggcgc agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat
     5761 aatgcaggtt ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc
     5821 attggtacat aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc
     5881 caacacaccc aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagct
     5941 tcccgccagc aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc
     6001 aattaccgct atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt
     6061 ctcccgcaga agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac
     6121 ccagatgttg cgtttttcct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta
     6181 accctgctgc cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag
     6241 tacaatattg gcctgcagcc tgggcttcac gcaggcgata ggcagaaagc cgggaaagat
     6301 cgacggaaat gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt
     6361 ccggccaggt accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga
     6421 tcacttcccg ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg
     6481 ggttttgtac cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa
     6541 cggtatcagg caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg
     6601 tgtcgaacat cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga
     6661 gacataattt ctctggcaaa agacagcgtt ggcagcgatg aacgcgatta ccgcgggcaa
     6721 gaaaaggacg tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gcgttttcgg
     6781 tcataagaga gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc
     6841 accccggtaa gattaaagag attcgttcag ccagctatcg aacggcgctt tcggtactgc
     6901 gccattaagc atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg
     6961 aattccaaag cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac
     7021 tttaccgcta cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc
     7081 gcaccacggt gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag
     7141 tgtttcaccg gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca
     7201 ttttgccgca tcttcgatcc gatcgtcggg aatgcgattg atggcctgac aatgggtaca
     7261 aacggtattc ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta
     7321 tgtttctaat atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc
     7381 aataggtatg atagtcgcag gctttggtac gaaatcatgg cgaaccgcgc ggacttcggg
     7441 taatctgcgc gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta
     7501 aaagcggcaa ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc
     7561 acaacccgcg taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc
     7621 gtcgccccgc ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc
     7681 cgtggcgcac ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg
     7741 gcatcagtgg taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa
     7801 cccgcgtcta cggcgaaaat gcctgtcagg cactgttcca gagccgtccg gaagcaattg
     7861 ttcgcgcctg gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg
     7921 cggcaaaccg caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca
     7981 cggaacatca cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc
     8041 agtgggtaag cctggcaggc gcgcaggatt gcgttctggc actggaaaat gaatctaacc
     8101 cgcataacct gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg
     8161 tgcaggatgc ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag
     8221 agcacgttca gccgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg
     8281 caggttacac cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc
     8341 tgccagcgaa aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac
     8401 gcgatccgaa cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata
     8461 tctctgtcgc aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa
     8521 tcagccgggt ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg
     8581 gtaatactgg catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca
     8641 gcacaaaatg gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg
     8701 ctttcagtac atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc
     8761 cccacaacaa aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag
     8821 taaacgtctc ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca
     8881 cagtattgag cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc
     8941 gggtgaagcc cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa
     9001 tggcaatgcc gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag
     9061 gatcctggcc gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa
     9121 agacatcttt ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct
     9181 gaagggtatt aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta
     9241 attcgttagc catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt
     9301 ctacagaaca aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg
     9361 gtaaaatttc acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat
     9421 cgatgtgtgg tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata
     9481 cactcaagca acaatggttt taccaattgg ccgcgacagg ctgaacaaat caaataattt
     9541 tgccggggag gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc
     9601 tgctgaactc tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa
     9661 aagcaggtta tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc
     9721 gtgaagttcc agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg
     9781 ttaacgttct gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc
     9841 agctgaccat ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc
     9901 agcgcgacgt tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac
     9961 taagcggaag agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca
    10021 atagaccagt aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg
    10081 ctgaaacaga tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag
    10141 caaaacgctg acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga
    10201 cagtgccgta acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc
    10261 ccaaaagctg attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt
    10321 ccacgccagc cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg
    10381 gtcaatcact gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc
    10441 cagactcatc cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct
    10501 aagggcgaga atattgttcg gtcctggcgt catagcggta atcagggtgt aagtccaaaa
    10561 agcacttaaa agggtcggtg tcacttgtta tctcctctgt tttggtagac agtacccgac
    10621 acggaacatt taaaaaaatg aatgttttta tattctagat tcgatatttt cgataggttt
    10681 ggggttatgg atctgcgccg ttttattacg cttaaaaccg tggtggaaga gggttccttt
    10741 ttgcgagctt cgcaaaaatt gtgctgtaca caatcgacgg tgacttttca tattcagcag
    10801 cttgagcagg agttttcagt ccagttattt gagaaaattg gccgacggat gtgccttacc
    10861 cgcgaaggaa aaaagttgct gccgcacatt tatgaactta cccgagtgat ggatacgctt
    10921 cgtgaggctg ccaaaaagga gtcagatccg gacggggaac tacgcgttgt ttcgggcgaa
    10981 acgttgctct cttaccgaat gccgcaggtg ttgcagcgtt ttcggcaacg cgcaccaaaa
    11041 gtgcgtttat cgttgcagtc gctgaactgt tacgtgatcc gtgatgcgct actgaatgat
    11101 gaggccgatg tcggcgtctt ttatcgcgta gggaatgatg atgccctgaa ccgacgagag
    11161 ttgggtgaac aatcgctggt gctggtggct tcaccacaaa ttgtagatgt cgattttact
    11221 gagccgggaa gacataacgc ctgtagcttt attatcaatg aaccacaatg tgtcttccgg
    11281 cagatatttg agagcacgct gcgtcagcgg cggatcacgg tggaaaacac cattgagctt
    11341 ataagtatcg aaagcatcaa gcgttgtgtc gcggcgaata tcggagtcag ctacctgccg
    11401 cgttttgcgg tggcaaaaga gttggagtgc ggagaattaa ttgaattgcc ttttggcgaa
    11461 cagtcgcaga ccattacggc catgtgcgca caccatgctg gaaaagcggt tagcccggcg
    11521 atgcacactt ttattcagtg tgttgaagag agttttgtgg ctggataaaa ataatgtcgg
    11581 gattaaaccc ggcatttaac ttgtcgacaa agtgcgcttt attcatgccg gatgcggcgt
    11641 gaacgcctta tccggcctac aagatcgtgc aaattcaata tattgcatga gccacgtagg
    11701 cctgataagc gtagcgcatc aggcagtttt gcgtttgcca gcacttttaa taccaggctt
    11761 aaacccggca ttgagattac tcttctgtcg tttgcggtgg cacgcccgtt ccgcttggtt
    11821 tacggcgctt accaatattt ttggtgtcgc gatggcgttt tttcacccgc ggtttctctt
    11881 tctctttagc ttttttcttc tcggcgcgtt ttgccagtac tttcttcgat ggcttgccgg
    11941 tctgcttctc gcttggcgca cgcgttttcg ggcgtaactc atcaataacg cgagctttaa
    12001 tcggctcttc aatgtaacgg ccaaccttgc ccagcagcaa atggtcatgg gcttccacca
    12061 gcgaaattgc ggtgccttta cgaccggcgc gcgcggtacg tccgatacgg tgcaaataag
    12121 tatcgccact gcgcggcata tcgaagttaa agacgtggct gacgtcagga atgtcgatac
    12181 cgcgcgcggc aacatcggtt gcaaccagta cgtttacgcg accttcggtc aaacgcttga
    12241 tcgcttcgtt acgcttgcct tgtaccatct caccttcgag atagcagttg ttgatgcccg
    12301 cttcgcgcag ccagtttgcc agctcatgca cacgttcacg cttacgcaca aacacaattg
    12361 agcgggtcgc ttccggctgt tttaacagat gcaccagcaa tgcggtttta tgttccaggt
    12421 catcggcgcg gtaataccac tgatgaattt ttttgcgctc acgggtggac ggattggcag
    12481 aaacctccac cggatcttcc agcagacgct cggcaaagtc ctgaatcgca tcgccttcca
    12541 gcgttgccga aaagagcagg gtctgtttac gccagcgcgt ttcgccagca atatgttcga
    12601 tatcctgagc gaagcccata tccagcatac ggtctgcttc gtcgaggatc agcgtttcaa
    12661 ccgcgcggca atcgaagttc tcttctttta tgtattgcag cagacgtccg gtcgtggcga
    12721 ccacgatgtc ctgattttcg ctgaacactt ccgcgtggtt catataggct acgccgccgg
    12781 tgatggtggc gatatccaga tgcgtatgtt tcgccagttc gcgggcatga tcggccacct
    12841 gcatcgccag ctcgcgagtt ggggtgagga tcaaaatacg cggcggaccg gatttcttac
    12901 gtgggaaatc gagcaggtgc tgcaacgctg gcagcagata cgccgccgtt ttaccggtgc
    12961 ctgtcggcgc agaaccgagt acatcacggc catcgagcgc aggcggaatg gcggcagcct
    13021 gaatggcggt cgggcgagtg aaacctttat cctggagggc ttccagcagg ctttcgtcga
    13081 gttcaagttc ggaaaaagtc gttacagtca tgttctacct ctgtgtgggg cgctgattat
    13141 agacgttacg gctgcaatct tcatctgttt gtatggatat cgcttctcgg gtattgttct
    13201 cacccggtgc tatcgccgtt tttcctgcaa ggttttattt tcatgtcaca gtctacatcc
    13261 gtgcttcgtc gtaatggatt tacttttaaa cagttttttg ttgctcacga tcgctgtgcg
    13321 atgaaagtgg gaacggatgg cattttgctg ggcgcatggg caccggtggc tggggtgaaa
    13381 cgttgccttg atatcggcgc gggtagcggg ttgctggcac tgatgctggc gcagcgaacc
    13441 agtgacagcg tgataattga tgccgttgag ctggaaagtg aagctgccgc gcaggcgcag
    13501 gaaaatatca accagtcccc gtgggcagag cggattaacg tccatacggc ggatattcag
    13561 cagtggctca cacagcagac agtacgcttc gatttaatca tcagtaaccc accttactat
    13621 cagcagggag tagagtgcgc gacacctcaa cgggaacagg ctcgctatac cactacgctt
    13681 gatcacccat cgctgctgac ctgtgcggcg gagtgcatta ccgaagaggg atttttctgc
    13741 gtggtgctgc cagagcaggt tggtaatggt tttactgaac tggcattaag catgggctgg
    13801 catttacgtt tgcggacgga tgtggcggaa aacgaagcgc gactgccgca tcgggtgctg
    13861 ctggcattct ccccgcaggc gggagaatgc tttagcgatc gcttagtcat tcgtgggcca
    13921 gaccagaact attccgaagc gtatacggcg ctgacccagg ctttttatct gtttatgtaa
    13981 tgattgccgg ggggaaggat cgatggaccg gaatgcgtga gcagttccgg gtaatccagc
    14041 gtaaaatgca ggccacgact ctccttgcgc atcatcgcac agcgaacaat caactcggca
    14101 acctgtacca gattacgcag ctccagcaaa ttattcgaca cgcggaagtg ggcgtaatat
    14161 tcgtctattt cctgttgcaa catattgata cgccgcaggg cgcgctcaag acgttttgtt
    14221 gtgcgcacaa tgccgacgta atcccacata aacagccgta gctcgtgcca gttatgctga
    14281 attactaccc gttcgtcagg gttctcaacg cggctttcat cccacggtgg taacgtactg
    14341 acgccgtggg catcaggcat acgtctggtg atatcttctg ccgccgacca gccatagacc
    14401 agacactcca gcaatgaatt cgaggccatg cggttagcgc cgtgtaagcc ggtataactc
    14461 acctcgccaa tggcatacaa gccctcgacg tccgtacgcc catgatcatc aaccattaca
    14521 ccaccgcagg tatagtgtgc agcaggcaca atcggtaccg gttcttgtgt gagatcaatc
    14581 cccagcccga gcaatttttc ataaatcatc gggaaatgct ggcgaataaa atcggcgggc
    14641 ttatggctga tgtcgaggaa catacaatct gcgccgaggc gtttcatttc atggtcaatg
    14701 gcgcgggcga caatatcgcg cggggccagt tcgccgcgct catcaaaatc gggcataaaa
    14761 cgcgtgccat ccgggcgctt gagataagcg ccttcgccgc gcagtgcttc cgttaacagg
    14821 aaattgcgtg cctgtgggtg atacagtgcg gtagggggga actgattaaa ttcgagattg
    14881 gcaacc
//
LOCUS       DAZRUF010000093        14853 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZRUF010000093 DAZRUF010000000
VERSION     DAZRUF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.093"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            100..342
                     /gene="yjbE"
                     /locus_tag="ACHXOL_002246"
     CDS             100..342
                     /gene="yjbE"
                     /locus_tag="ACHXOL_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR6809218.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            456..1094
                     /locus_tag="ACHXOL_002247"
     CDS             456..1094
                     /locus_tag="ACHXOL_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR6809219.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1091..1828
                     /locus_tag="ACHXOL_002248"
     CDS             1091..1828
                     /locus_tag="ACHXOL_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR6809220.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1828..3924
                     /locus_tag="ACHXOL_002249"
     CDS             1828..3924
                     /locus_tag="ACHXOL_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR6809221.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3971..4249)
                     /locus_tag="ACHXOL_002250"
     CDS             complement(3971..4249)
                     /locus_tag="ACHXOL_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809222.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4250..4393
                     /locus_tag="ACHXOL_002251"
     CDS             4250..4393
                     /locus_tag="ACHXOL_002251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809223.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            4519..4929
                     /gene="psiE"
                     /locus_tag="ACHXOL_002252"
     CDS             4519..4929
                     /gene="psiE"
                     /locus_tag="ACHXOL_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR6809224.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4973..6448)
                     /gene="xylE"
                     /locus_tag="ACHXOL_002253"
     CDS             complement(4973..6448)
                     /gene="xylE"
                     /locus_tag="ACHXOL_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR6809225.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6820..7710)
                     /gene="malG"
                     /locus_tag="ACHXOL_002254"
     CDS             complement(6820..7710)
                     /gene="malG"
                     /locus_tag="ACHXOL_002254"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR6809226.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7725..9269)
                     /gene="malF"
                     /locus_tag="ACHXOL_002255"
     CDS             complement(7725..9269)
                     /gene="malF"
                     /locus_tag="ACHXOL_002255"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR6809227.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9423..10613)
                     /gene="malE"
                     /locus_tag="ACHXOL_002256"
     CDS             complement(9423..10613)
                     /gene="malE"
                     /locus_tag="ACHXOL_002256"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR6809228.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10978..12093
                     /gene="malK"
                     /locus_tag="ACHXOL_002257"
     CDS             10978..12093
                     /gene="malK"
                     /locus_tag="ACHXOL_002257"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR6809229.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12165..13505
                     /gene="lamB"
                     /locus_tag="ACHXOL_002258"
     CDS             12165..13505
                     /gene="lamB"
                     /locus_tag="ACHXOL_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR6809230.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13657..14577
                     /gene="malM"
                     /locus_tag="ACHXOL_002259"
     CDS             13657..14577
                     /gene="malM"
                     /locus_tag="ACHXOL_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR6809231.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3683 a         3821 c         3906 g         3443 t
ORIGIN      
        1 ttccccgcgc cgtaattcgc atgctttagt tgtgtatact cgatcccgcc cgaaatgttt
       61 ttgggtaaat ctccattcat tcaatgaagg gaaattgtta tgaaaaaagt tctgtatggc
      121 atttttgcca tatctgcgct tgcggcgact tctgtgtggg ctgcacctgt acaggtgggc
      181 gaagcggcag gggcggcagc aacgtcggtt tcggcgggga gttcctccgc gaccagcgtc
      241 agcaccgtaa gctcggcggt gggtgtcgcg ctcgcggcaa ccggtggcgg tgatggttct
      301 aataccggga ccacaacaac cacaaccacc agtacccagt aataaagtat gtatccccaa
      361 aataattcga gtcattgcat ctgtggcttg aaatatgaag ggattaacca taaccacact
      421 ccggtgtggt tattctgccc ctctggagaa gagtcgtgaa gcgacctgca ctcattctta
      481 tctgcctgtt attacaggcc tgttcagcca cgactaaaga gctgggcaat tcactgtggg
      541 acagtctgtt cggcacgcca ggcgtacagc tgacggacga tgatattcaa aatatgccct
      601 acgccagcca gtacatgcag cttaatggcg ggccgcaatt gtttgtggtg ctggccttcg
      661 ctgaagacgg acaacaaaaa tgggtcactc aggatcaagc cactcttgtc acacaacatg
      721 gtcgtctggt gaagactttg cttggcggcg acaacctgat tgaagtgaat aaccttgccg
      781 ccgacccgct gattaaaccc gcacaaattg ttgatggtgc aacatggacg cgcacgatgg
      841 gctggaccga gtaccagcag gtacgctacg ccaccgcacg ctcagtcttc aaatgggatg
      901 gcaccgatac cgtcaaggtc ggcagcgatg aaaccctggt tcgcgtgctg gacgaagaag
      961 tctccaccga ccaggcgcgc tggcataacc gctactggat cgacagcgaa gggcaaattc
     1021 gccagtcgga acagtatctc ggcgcggatt atttcccggt gaaaaccact ctcatcaagg
     1081 cggcaaaaca atgattaaac aaactattgt cgcgttgctt ttgagtgtgg gagcgtcatc
     1141 ggtcttcgcg gcaggaaccg tcaaggtgtt cagcaatggc agcagtgagg ccaaaacgct
     1201 aacgggcgca gagcatttaa tcgatctggt agggcaacct cgactggcaa acagctggtg
     1261 gcctggtgcg gtgattagcg aagagctggc aacggcggca gcattgcgtc agcagcaggc
     1321 gttgctgaca cggctggcag aacagggcgc agattccagc accgacgatg ccgccgccat
     1381 taacgcctta cgccagcaaa tccaggcatt ggaagtgaca ggcaggcaaa aaatcaatct
     1441 tgatcccgat atcgtccgcg ttgccgaacg cggtaacccg ccgttgcagg gcaactacac
     1501 gctgtgggtc ggaccaccgc cgtccacggt cacgttgttc gggcttatca gccatcctgg
     1561 caatcagcca ttcactccag gtcgcgacgt ggcgagctat ctctctgacc agagcctgct
     1621 cagcggtgcg gatcgcagct acgcgtgggt cgtttacccg gacggacgca cgcaaaaagc
     1681 gccggtggct tactggaaca agcgtcacgt agagccgatg cccggcagca ttatttatgt
     1741 tggcctcgca gactccgtct ggagtgagac gcctgatgcc cttaacgccg acattcttca
     1801 gactctgacg cagcggatac cccaataatg aaaaaaagac atctgcttag cttactggcg
     1861 ctgggcatta gcacagcttg ctacggcgaa acatatcctg cgcccattgg tccgtcgcag
     1921 tcggatttcg gtggcgtagg attattacaa actcccaccg cgcgtatggc acgggaaggg
     1981 gagttgagtc tgaactatcg cgataacgat cagtaccgtt attactcagc ttcagtgcaa
     2041 ctcttcccgt ggctggaaac aacgctgcgt tacaccgacg tgcgcacccg gcagtacagc
     2101 agcgtcgaag cgttctctgg cgatcaaacg tataaagata aagccttcga tctcaaactg
     2161 cgtttgtggg aagagagtta ctggctgccg caagtggcgg ttggtgcgcg ggatatcggc
     2221 ggtacggggc tgtttgatgc ggaatatctt gttgccagca aagcctgggg gccgttcgat
     2281 tttacgctcg gcctgggctg gggatatctg ggcaccagcg gtaatgtgaa aaatccgctc
     2341 tgttcagcca gtgataaata ttgctatcgc gataacagct acaaacaagc gggatctatc
     2401 gacggcagtc agatgttcca cggcccggcc tcactgtttg gcggcgtgga ataccagacg
     2461 ccctggcaac cgctgcgcct gaaactggag tatgaaggta ataattatca gcaggatttt
     2521 gccgggaagc tggagcaaaa aagtaagttt aacgtcgggg cgatttatcg cgttaccgat
     2581 tgggccgacg ttaaccttag ctatgaacgt ggcaacacct ttatgtttgg cgtcacgctg
     2641 cgcaccaact ttaacgatct gcgcccgtcc tacatcgata acgcccgccc gcaatatcaa
     2701 ccgcagccgc aggatgccat tttgcagcac tcggtggtgg cgaatcagtt aacgttgctg
     2761 aaatacaatg ccggacttgc cgatccgcag atccaggcga aaggcgatac gctgtatgtt
     2821 accggcgagc aggtgaaata tcgtgattcg cgcgaaggga tcatccgtgc taatcggatc
     2881 gtgatgaacg atctgccgga tgggatcaaa acgatccgca ttacggaaaa tcgccttaac
     2941 atgccgcagg tgacgacgga aaccgatgtc gccagcctga aaaatcatct cgccggagag
     3001 ccgttgggcc acgaaacgac gctggcgcaa aaacgcgtcg agccagtggt tccgcagtcc
     3061 accgagcagg gctggtatat cgacaaatca cgctttgatt tccatatcga tccggtgctg
     3121 aaccagtcgg tcggtggccc ggaaaacttt tacatgtatc agctgggcgt gatgggaacg
     3181 gcagatttgt ggctgacgga ccatctgctg accatcggca gcctgtttgc aaatcttgcc
     3241 aacaactacg acaagtttaa ctacaccaat ccgccacagg actcgcactt accgcgcgtt
     3301 cgtacccatg tgcgcgagta tgtgcagaac gatgcctatg tgaataacct gcaagccaac
     3361 tacttccagc atctgggcaa cggcttctac ggtcaggtct atggaggtta tctcgaaacc
     3421 atgtttggcg gtgcgggggc tgaagtgttg tatcgtccgc tggacagcaa ctgggcgttt
     3481 ggtctggatg ccaactacgt taaacagcgc gactggcgta gtgcaaaaga tatgatgaaa
     3541 ttcaccgact acagcgtgaa aaccggacat ctgaccgcct actggacgcc atctttcgcc
     3601 caggacgtgt tagtgaaagc cagcgtcggg cagtatctgg caggggataa aggcggcacg
     3661 ctggagatcg ccaaacgctt tgatagcggc gtggtggtgg gtggctatgc cacgatcacg
     3721 aatgtttcaa aagaggagta cggcgaaggg gacttcacca aaggcgtgta tgtctcggta
     3781 ccgttggatc tcttctcgtc tggcccgaca cgcagccgtg cggcgattgg ctggacgccg
     3841 ctgacgcgtg acggtggtca gcaacttggg cgtaagttcc agttgtatga tatgaccagc
     3901 gaccgtagcg tcaatttccg ctaagtcatg ggaaaggtgc cagttttcgc aatcactggc
     3961 accctcattc ttaatggcct gttgagccat caggaacgca gttcaccccc ggtttgcgat
     4021 ctggtgtgta aaggcgaaaa cattctgtct tcctgtccgt cgatatcggt tgcaggtctg
     4081 gttgcctgag cgactgggcc atcattgggc tggagacatt gctttgcggt aggtcggctt
     4141 tatcagcagt accgagcgga ccagcataag caggaagaac agagactgat aacatcaaag
     4201 cagcaaaata aggcttcatt tttaccacct ttatcaggtt acgtttcatt tgttccagag
     4261 gaacattgtc gatttttcgc gcattgctgg tggctgggaa tcacctgaat gggtgatttt
     4321 tgaattaccg gcttgggtgc ggtttgtctt gccggatgcg ccgccaggcg cggcttatcc
     4381 ggcctacggg taggtatatc cggctttggt ggaggcgcgc tccaaatcca ggttgaacaa
     4441 aacatacaca aaaaatatag atctccgtca catttttgcg ttatacagga agctcgccac
     4501 tgtgaaggag gtactgctat gacgtcactc tctcgtccgc gcgtggagtt tatctccacc
     4561 attttgcaga ccgtactcaa tcttggcctg ctgtgcctcg gcctgatttt ggttgtcttc
     4621 ctcggcaaag aaacggtgca tctggcagat gtgctgttcg cgccagaaca aaccagcaaa
     4681 tatgagctgg tagaaggact ggtggtttac tttctctatt tcgaatttat cgcgctgatt
     4741 gtgaagtact ttcagtccgg ttttcacttc ccgctacgtt actttgtcta tattgggatc
     4801 accgccattg tgcgcttgat catcgtcgat cataaatcgc cactggatgt gttgatctac
     4861 tcggctgcga tcctgttact ggtgatcact ctgtggctgt gtaattcgaa acggctgaag
     4921 cgggagtaaa aagaaagcac gccgaactgg cgcggcgtgc tggacaggaa gattacagcg
     4981 tagcagtttg ttgtgttttc ttcgtttccg gttcccagag ggcttccagc tcctcaaggg
     5041 ttttaccttt ggtttccggg acaaatttcc acataaatag tgctgccaga acgcccatac
     5101 acccgtaaat ccagtaggag aaaccgttgt ggaaatgggc caccagccag gagtttttgt
     5161 ccatcatcgg gaaggtccag gagacgaagt agttcgccag ccactgggcc gccaccgcga
     5221 ttgccagcgc tttaccacga atagcattcg ggaagatttc cgacagcagt acccagcata
     5281 ccggacccca ggacatggca aaggcagcaa catagaacag catcgacagt agcgccacaa
     5341 tacccggtgc ctgagtgtaa aacgcggtac cgaggctaaa cataccgatt gccattccga
     5401 gtgcgccgat aatttgcagt ggcttacgac caaatttatc caccgtcata attgccagaa
     5461 cggtgaaggt gaggttgata actccgacaa taatggtctg caacagcgcg atatccgtgc
     5521 tggcccccag cgttttgaac acttccggcg cgtagtacag caccacattg atgccgacaa
     5581 attgctggaa gatggagagc ataacgccga tgacaatcac gcccacgcca aacatcagca
     5641 gacgaccacc ggttttgcgg ccatgatcca gggagtgttt aatttcctgt actgcctgag
     5701 ttgcaagcgt gttgcccata attttgcgca ggatactttc cgcctgttct tgcttgccgc
     5761 gtgacatcag ccagcgagga ctttctggca cggtatacag cagcattaag aacagcagtg
     5821 cagggataca ttccgaggca aacatataac gccagccgtc agtattcagc cagctggcat
     5881 caccggaacg ggcaataaaa tagtttacgc agtaaactaa aagttgcccg aaaataatcg
     5941 caaactggtt aaatgaaacc agttttccgc gaatatgagc tggagccagt tccgcaatat
     6001 acattggtga aagcattgag gctaaaccaa cgccaatacc gccaataata cgataaataa
     6061 caaattccgg gacataacct gccagataaa taggtactgt gttgtccggg tttatagagg
     6121 taaaaccaag ttctggccag gcagaaccta caccagaaat aaaaaacagg acagcagcaa
     6181 tcttaagtga atcacgacgg ccgaagcggt tactgcaata accaccgagg gcaccgccga
     6241 tgatgcaacc aatcagagcg ctggctacgc aaaatcctaa cagggagttg gcagcggatt
     6301 cacttaagtt ttgtggagca acaaagacgg tattgagtga ctcaacagta ccggaaataa
     6361 cggcggtgtc gtagccaaat aataaaccac ctaatgtagc gactaaggta atcgaaaata
     6421 tataactgga attatactgg gtattcattc agacctgcct tagaccattc tgatctttta
     6481 tcagtgcatt gatgttggaa ttgtcaggca gttaaggcaa ttatcatttt ttacaatgcg
     6541 tttacgtaat gtcttttctg tgatcttaat tgtgataatt atcaaaaatt gatgaaaaca
     6601 atactattgc cgtgactcag agcacgaaag agaattatcg taagtgggaa aacaaataac
     6661 gtaaaaataa taagctctat gatggaaata ttaaccggcg aacgattcag attgcagacg
     6721 aaagaaaaaa aggcgctccg tggagcgccg aataacagtc acaagttggg ataacgtaag
     6781 ttgagggtgc agcggcataa cattggcaga acaacatctt taacctttca caccacctgc
     6841 cgtcaggccg ttgaccagcc agcgttgagc cagcaagaag acgatggtga tcggtaatgc
     6901 agacatcacg gcagcggcgg caaagtcacc ccacaggtag ttttgcgggt tgaggtattg
     6961 ctgcatcccc acggccaggg tgtagctgtt tacgtcacgc agtaacagcg acgcgaccgg
     7021 aacttcagta atggcagcga taaacgacag gatgaatacc accgccagaa tcggtactga
     7081 cagcggcaac aggacaaggc ggaaggcctg ccacggtgtc gcaccatcca gcgcagccgc
     7141 ttcttccagc gaactatcga tggtttcgaa atagcctttg atggtccaga catgcagcgc
     7201 aatcccaccc agatacgcga aaattacgcc gccgtgagta ttcaggccaa tgaatggaat
     7261 gtactcaccc agacgatcaa acaacgcata caacgcgacc agtgaaagta ctgccgggaa
     7321 catctggaaa atcagcattc ctttcagcag cgtcgctttg cctggaaagc gcatacgggc
     7381 gaaagcgtag gcgcaggtgg tagagagcgc gacaatgcca atcgcggaaa tcccggcgac
     7441 ctttaccgag ttccatagcc acagcagtac cgggaatggc ggtggcgtaa tgcgaccatc
     7501 agcctgttcc acgctaaaac ctaaagccag tttccagtga tcccaggaga tttgctccgg
     7561 gatcaggctg ccgatcgcaa agttcccctg gcgcagtgag atagcgacga ccatcagcag
     7621 cgggaacata atcgccgcga taaaaagtag cagtagcagg tgagtaataa acaaacgtgc
     7681 tttttgcgat ttcggttgga ccattgccat ttttgttatc tcccttaatc aaacttcatt
     7741 cgcgtggctt tcaggttcac tatcgccagc gcacccacca gcaggaagag cagcgtggca
     7801 attgctgcag ccagaccgaa gtcctgaccc ccgccgcctt caaaagcgat acggtaggtg
     7861 tagttaacaa gcaggtcggt ataaccggct ggcgtggtcg tgccaagacg atccgggccg
     7921 ccgttggtta acagttgaat cagcacgaag ttgttaaagt taaaggcgaa gctggcgatc
     7981 atcagcggcg tcagcggttt aatcagcagc ggcagcgtaa tcttaaagaa gttctggaac
     8041 ggacctgcgc catccattgc tgaggcttca tacaaatcgt ccggaatcgc tttcagcaag
     8101 cccatgcaga ggatcatcat gtacggataa cccagccagg tattgacgat aattagcatc
     8161 gtgcgggcgg tggtcggatc gctgaaccag gcgggcttca cgccaaacag cgcgctcaac
     8221 atcatgttga tttcaccgaa gctctggtta aacaaccctt tgaaaatcaa gattgaaatg
     8281 aatgatggca ccgcgtaggg cagaatcagc agaacgcgat agaccgcttt gccgcgcaat
     8341 gcttcccact gcaccagaca tgccagaacc atgccgaccg ccaccgttaa aaagacagtg
     8401 atcagcgaga acaccacggt ccagacgaaa atggcgagga acggtttctg aatgccttcg
     8461 tcggtgaaga cgcgggtaaa gtttttccag ccagtagtca cggtgtaacc ggggcttagc
     8521 ttttcatcac cccagttgcc gtcggcggta atggactggt aaaagccaat ttggttattc
     8581 ggacgatatt tcacgccgct ctgattattc gtcaacgtgc cgtcaccgtc gagtgtgtag
     8641 agcggctgcg tgccagaaaa ctggcgcagg gagctcatca tcactttgtt gccatccggc
     8701 agaatggcgg taatgtcact cagcgcctga cgattctggg taatcacgcg tagattcgcg
     8761 cgttcgcctt cgggctgggc ggtcgtttct ttcagttgca gtttttgttc gccgccaaat
     8821 ttaaacgcgt cggagaggta atttttgccg atttcgccgt cactgagcgc cagttgccac
     8881 tcatcgcccg ccgggtaaag accaaagtta taggttttgc ctgcttgcca ggagcgatct
     8941 aacaacactt cctgcgcaca ttcaaaagtc agctggttag tgctgctgta gttggtgaag
     9001 gcaatggcga tggtgcagac cagagggaag aggacgaata atcccattcc agccattccc
     9061 gggtaaacat agcgccaggc gtaggcttta cgattggcga aaatatacag ccccgctgaa
     9121 ctcaatatca gcgtggtaat ggcgaacagg tattcccctt gtgcgtacat taaaacaaca
     9181 aggtaaccca ccagcaggcc gagcagacct agcactgacc atttcagcgc gtcgctttgc
     9241 caccaatgtt tctttttaat gacatccatg gggttcttcc tcattccagg acggataagg
     9301 ctttcacgcc ttatccgaca acaactgcct gatgcgacac tgatgcgtct tatcaggcct
     9361 acatgcgttt cagttttgta ggccggacaa ggcgttcacg ccgcatccgg catttcacag
     9421 cattacttgg tgatacgagt ctgcgcgtct ttcagggctt catcgacagt ctgacgaccg
     9481 ctggcggcgt tgatcaccgc agtacgcacg gcataccaga aagcggacat ctgcgggatg
     9541 ttcggcatga tttcaccttt ctgggcgttt tccatggtgg cggcaatacg tggatctttc
     9601 gccaactctt cctcgtaaga cttcagcgcc actgcaccca gcggtttgtc tttattgacc
     9661 gcttccagac cttcgtcagt cagcagatag ttttcgagga actctttcgc cagctctttg
     9721 ttcggactgg cggcgttaat accggcgctc agcacgccaa cgaacggttt agatggttga
     9781 cccttgaagg tcggcagtac cgttacacca taattcactt tgctggtgtc gatgttggac
     9841 catgcccacg ggccgttgat ggtcatcgct gtttcgcctt tattaaaggc agcttctgcg
     9901 atggagtaat cggtgtctgc attcatgtgt ttgtttttaa taaggtcaac caggaaggtc
     9961 agacccgctt tcgcgccagc gttatccacg gccacgtctt taatatcgta cttgccgttt
    10021 tcatacttga acgcataacc cccgtcagca gcaatcagcg gccaggtgaa gtacggttct
    10081 tgcaggttga acatcagcgc gctcttacct ttcgctttca gttctttatc cagcgccggg
    10141 atctcttccc aggtttttgg tgggttcggc agcagatctt tgttataaat cagcgataac
    10201 gcttcaacag cgatcgggta agcaatcagc ttgccgttgt aacgtacggc atcccaggta
    10261 aacggataca gcttgtcctg gaacgctttg tccggggtga tttcagccaa caggccagat
    10321 tgagcgtagc caccaaaacg gtcgtgcgcc cagaagataa tgtcagggcc atcgccagtt
    10381 gccgcaacct gagggaattt ctcttccagt ttatccggat gctcaacggt gactttaatt
    10441 ccggtatctt tctcgaattt cttaccgact tcagcgagac cgttatagcc tttatcgccg
    10501 ttaatccaga ttaccagttt accttcttcg attttggcga gagccgaggc ggaaaacatc
    10561 atcgtcgtta atgcggataa tgcgaggatg cgtgcacctg tttttatttt cataatctat
    10621 ggtccttgtt ggtgaagtgc tcgtgaaaac acctaaacgg actctagttt ctttatacgg
    10681 caacctcttt ccatcctcct tgcccctacg ccccaccgtt gctttgtgtg atctctgtta
    10741 cagaattggc ggtaatgtgg agatgcgcac ataaaatcgc cacgattttt gcaagcaaca
    10801 tcacgaaatt ccttacatga cctcggttta gttcacagaa gccgtgttct catcctcccg
    10861 cctcctcccc cataaaaaag ccagggggtg gaggatttaa gccatctcct gatgacgcat
    10921 agtcagccca tcatgaatgt tgctgtcgat gacaggttgt tacaaaggga gaagggcatg
    10981 gcgagcgtac agctgcaaaa tgtaacgaaa gcctggggcg aggtcgtggt atcgaaagat
    11041 atcaatctcg atatccatga aggtgaattc gtggtgtttg tcggaccgtc tggctgcggt
    11101 aaatcgactt tactgcgcat gattgccggg cttgagacga tcaccagcgg cgacctgttc
    11161 atcggtgaga aacggatgaa tgacactccg ccagcagaac gcggcgttgg tatggtgttt
    11221 cagtcttacg cgctctatcc ccacctgtca gtagcagaaa acatgtcatt tggcctgaaa
    11281 ctggctggcg caaaaaaaga ggtgattaac caacgcgtca accaggtggc ggaagtgctg
    11341 caactggcgc atttgctgga tcgcaaaccg aaagcgctct ccggtggtca gcgtcagcgt
    11401 gtggcgattg gccgtacgct ggtggccgag ccaagcgtat ttttgctcga tgaaccgctc
    11461 tccaacctcg atgctgcact gcgtgtgcaa atgcgtatcg aaatctcccg tctgcataaa
    11521 cgcctgggcc gcacaatgat ttacgtcacc cacgatcagg tcgaagcgat gacgctggcc
    11581 gacaaaatcg tggtgctgga cgccggtcgc gtggcgcagg ttgggaaacc gctggagctg
    11641 taccactatc cggcagaccg ttttgtcgcc ggatttatcg gttcgccaaa gatgaacttc
    11701 ctgccggtaa aagtgaccgc caccgcaatc gatcaagtgc aggtggagct gccgatgcca
    11761 aatcgtcagc aagtctggct gccagttgaa agccgtgatg tccaggttgg agccaatatg
    11821 tcgctgggta ttcgcccgga acatctactg ccgagtgata tcgctgacgt catccttgag
    11881 ggtgaagttc aggtcgtcga gcaactcggc aacgaaaccc aaatccatat ccagatccct
    11941 tccattcgtc aaaacctggt gtaccgccag aacgacgtgg tgttggtaga agaaggtgcc
    12001 acattcgcta tcggcctgcc gccagagcgt tgccatctgt tccgtgagga tggcactgca
    12061 tgtcgtcgac tgcataagga gccgggcgtt taagcacccc acaaaacaca caaagcctgt
    12121 cacaggtgat gtgaaaaaag aaaagcaatg actcaggaga tagaatgatg attactctgc
    12181 gcaaacttcc tctggcggtt gccgtcgcag cgggcgtaat gtctgctcag gcaatggctg
    12241 ttgatttcca cggctatgca cgttccggta ttggctggac aggtagcggc ggtgaacaac
    12301 agtgtttcca gactaccggt gctcaaagta aataccgtct tggcaacgaa tgtgaaactt
    12361 atgctgaatt aaaattgggt caggaagtgt ggaaagaggg cgataagagc ttctatttcg
    12421 acactaacgt ggcctattcc gtcgcgcaac agaatgactg ggaagctacc gatccggcct
    12481 tccgtgaagc aaacgtgcag ggtaaaaacc tgatcgaatg gctgccaggt tccaccatct
    12541 gggcaggtaa gcgcttctac caacgtcatg acgttcatat gatcgacttc tactactggg
    12601 atatttctgg tcctggtgcc ggtctggaaa acatcgatgt tggcttcggt aaactctctc
    12661 tggcagcaac ccgctcctct gaagctggtg gttcttcctc ttttgccagc aacaatattt
    12721 atgactatac caacgaaacc gcgaacgacg ttttcgatgt gcgtttagcg cagatggaaa
    12781 tcaacccggg cggcacatta gaactgggtg tcgactacgg tcgtgccaac ctgcgtgata
    12841 actatcgtct ggttgatggc gcatcgaaag acggctggtt attcactgct gaacatactc
    12901 agagtgtcct gaagggcttt aacaagtttg ttgttcagta cgctactgac tcgatgacct
    12961 cgcagggtaa aggtctgtcg cagggttctg gcgtcgcgtt tgataacgaa aaatttgcct
    13021 acaatatcaa caacaacggt cacatgctgc gtatcctcga ccacggtgcg atctccatgg
    13081 gcgacaactg ggacatgatg tacgtgggta tgtaccagga tatcaactgg gataacgaca
    13141 acggcaccaa gtggtggacc gtcggtattc gcccgatgta caagtggacg ccaatcatga
    13201 gcaccgtgat ggaaatcggc tacgacaacg tcgaatccca gcgcaccggc gacaagaaca
    13261 atcagtacaa aattaccctc gcacaacaat ggcaggctgg cgacagcatc tggtcacgcc
    13321 cggctattcg tgtcttcgca acctacgcca agtgggatga gaaatggggt tacgactaca
    13381 acggcgatag caaggttaac ccgaactacg gcaaagccgt tcctgctgat ttcaacggcg
    13441 gcagcttcgg tcgtggcgac agcgacgagt ggaccttcgg tgcccagatg gaaatctggt
    13501 ggtaatagca aaacctgggc cggataaggc gtttacgccg catccggcat aaaaacaggt
    13561 tgtcattatc tgaaaggggc gaaagcccct ctgattatcg ggtttagcgc gctattgcct
    13621 ggctaccgct gagctccaga ttttgaggtg aaaacaatga aaatgaataa aagtctcatc
    13681 gccctctgtt tatcagcagg gttactggca agcgcgcctg gaattagcct tgccgatgtt
    13741 aactacgtac cgcaaaacac cagcgacgcg ccagccattc catctgccgc gctgcaacaa
    13801 ctcacctgga caccggtcga tcaatctaaa acccagacca cccaactggc gaccggcggc
    13861 caacaactga acgttcccgg catcagtggt ccggttgctg cgtacagcgt cccggcaaac
    13921 attggcgaac tgaccctgac gctgaccagc gaagtgaaca aacaaaccag cgtttttgcg
    13981 ccgaacgtgc tgattcttga tcagaacatg accccatcag ccttcttccc cagcagttat
    14041 ttcacctacc aggaaccagg cgtgatgagt gcagatcggc tggaaggcgt tatgcgcctg
    14101 acaccggcgt tggggcagca aaaactttat gttctggtct ttaccacgga aaaagatctc
    14161 cagcagacga cccaactgct cgacccggct aaagcctatg ccaaaggcgt cggtaactcg
    14221 atcccggata ttcccgatcc ggttgctcgt cataccagcg atggcttact gaaactgaaa
    14281 gtcaaaacga actccagctc cagcgtgttg gtaggaccac tatttggttc ttccgctcca
    14341 gctccggtta cggtaggcaa cacggcggca ccagctgtgg ctgcacctgc tccggtgccg
    14401 gtgaagaaaa gcgagccgat gctcaacgac acggaaagtt attttaatac cgcgatcaaa
    14461 aacgctgtcg cgaaaggtga tgttgataag gcgttaaaac tgcttgatga agctgaacgc
    14521 ctgggatcga catctgcccg ttccaccttt atcagcagtg taaaaggcaa ggggtaatta
    14581 cgccccacag tgctgatttt gcaacaactg gtgcgtctcc tggcgcacct ttttttatgc
    14641 ttccttcctg ggatatgagc gattttttat agtacttcac ttcttcttca ctaagaatgt
    14701 ccattatctc aatgccttat cagagattct tttcctttcg ccggtagtgt ctggacgctc
    14761 aggctacttt tccaggttat tttatttctg tattacagag gttttatgat aggtcatatc
    14821 acaaattctg gcggcaataa ctctttgatg aaa
//
LOCUS       DAZRUF010000094        14775 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZRUF010000094 DAZRUF010000000
VERSION     DAZRUF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.094"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHXOL_002260"
     CDS             complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHXOL_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR6809232.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            739..2937
                     /gene="priA"
                     /locus_tag="ACHXOL_002261"
     CDS             739..2937
                     /gene="priA"
                     /locus_tag="ACHXOL_002261"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR6809233.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASEIPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3093..4118
                     /gene="cytR"
                     /locus_tag="ACHXOL_002262"
     CDS             3093..4118
                     /gene="cytR"
                     /locus_tag="ACHXOL_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR6809234.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHXOL_002263"
     CDS             4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHXOL_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR6809235.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5262..5792
                     /gene="hslV"
                     /locus_tag="ACHXOL_002264"
     CDS             5262..5792
                     /gene="hslV"
                     /locus_tag="ACHXOL_002264"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR6809236.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5802..7133
                     /gene="hslU"
                     /locus_tag="ACHXOL_002265"
     CDS             5802..7133
                     /gene="hslU"
                     /locus_tag="ACHXOL_002265"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR6809237.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7200..8126
                     /gene="menA"
                     /locus_tag="ACHXOL_002266"
     CDS             7200..8126
                     /gene="menA"
                     /locus_tag="ACHXOL_002266"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR6809238.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8219..8704
                     /gene="rraA"
                     /locus_tag="ACHXOL_002267"
     CDS             8219..8704
                     /gene="rraA"
                     /locus_tag="ACHXOL_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR6809239.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHXOL_002268"
     CDS             complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHXOL_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR6809240.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9460..10305
                     /gene="glpF"
                     /locus_tag="ACHXOL_002269"
     CDS             9460..10305
                     /gene="glpF"
                     /locus_tag="ACHXOL_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR6809241.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10328..11839
                     /gene="glpK"
                     /locus_tag="ACHXOL_002270"
     CDS             10328..11839
                     /gene="glpK"
                     /locus_tag="ACHXOL_002270"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR6809242.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11969..12979
                     /gene="glpX"
                     /locus_tag="ACHXOL_002271"
     CDS             11969..12979
                     /gene="glpX"
                     /locus_tag="ACHXOL_002271"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR6809243.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13076..13822
                     /gene="fpr"
                     /locus_tag="ACHXOL_002272"
     CDS             13076..13822
                     /gene="fpr"
                     /locus_tag="ACHXOL_002272"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR6809244.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHXOL_002273"
     CDS             complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHXOL_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR6809245.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14282..14581)
                     /locus_tag="ACHXOL_002274"
     CDS             complement(14282..14581)
                     /locus_tag="ACHXOL_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR6809246.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
BASE COUNT         3556 a         3798 c         3976 g         3445 t
ORIGIN      
        1 gcaccgagca gcggattgac cggatggccg gacgtcaccc cgccggggca caccgaacag
       61 gccacgccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga
      181 aataaataag aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca
      241 ccccggaatt tttcagaatt tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg
      301 gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag
      361 cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac
      421 ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta
      481 ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga
      541 aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt
      601 aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac
      661 cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt
      721 tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt
      781 acctttgact atctgctgcc agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg
      841 ccgtttggca aacagcagga gcgcatcggg gttgtggtat cagttagcga tgtcagtgag
      901 ctgccgctta acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact
      961 cactccgtct ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat
     1021 gtgctgtttc atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg
     1081 atgtggtact ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc
     1141 tcacccaagc aacaacaggc gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag
     1201 gttgccacgc tcgaatttaa tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt
     1261 gatttagcaa gtgaaatacc agagtttagc gactggcgaa cgaactatgc cgtttctggt
     1321 gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca
     1381 gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat
     1441 ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa
     1501 atcggcctga caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa
     1561 gttctgcatt ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat
     1621 ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc
     1681 ggcgtgattg tcattgacga agagcacgac agttcctaca agcagcagga aggctggcgc
     1741 tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt
     1801 ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg
     1861 ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta
     1921 aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat
     1981 ttacaggcca acaatcaggt tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta
     2041 ctgtgccacg actgcggctg gattgccgaa tgcccacgtt gcgatcacta ctacacgctg
     2101 catcaggcgc aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc
     2161 cagtgccctt cctgcggttc cacgcacctg gtacccgtgg ggctgggcac cgaacagctt
     2221 gaacagacgc ttgcgccgct gttccctgat gtgcccattt ctcgtatcga ccgcgatacc
     2281 accagccgca aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg
     2341 attttgatcg gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg
     2401 gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc
     2461 gctcagcttt acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg
     2521 gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac
     2581 gacgcctttg ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc
     2641 agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa
     2701 caactgcgta atctgatcct ctccagcccg ctggcagacg acaaattgtg ggttctcggt
     2761 ccggttccgg cactggcacc taaacgtggc ggtcgctggc gctggcagat attgttgcag
     2821 cacccttccc gcgtgcgctt gcaacacatc attaacggta cgctggcact catcaataca
     2881 ataccggatt cccgtaaggt gaaatgggtg ctggatgttg atccgattga gggttaaacc
     2941 gctcacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct
     3001 gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt
     3061 agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg
     3121 cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat
     3181 taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg
     3241 aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca
     3301 ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg
     3361 aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc
     3421 agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc
     3481 tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg
     3541 tgatggcgaa cgaattcgca ccggaactgg agctacctac agttcatatc gacaatctga
     3601 ccgccgcatt tgatgcagta aattatctat atgagcaagg gcataaacgg attggctgta
     3661 tagccggtcc cgaagagatg ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc
     3721 tgcgtcgctg cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg
     3781 aagccggaag caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct
     3841 tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga
     3901 aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg
     3961 atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt
     4021 tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg
     4081 aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt
     4141 gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc
     4201 gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga
     4261 aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc
     4321 gctattgctg ccgccgtgct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac
     4381 aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca
     4441 aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt
     4501 gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa
     4561 caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt
     4621 gaagttccgt ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt
     4681 caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa
     4741 agctggcagc agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag
     4801 tctaaaccgg cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg
     4861 accgcgcagt cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa
     4921 ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc
     4981 gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc
     5041 accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca
     5101 gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc
     5161 gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt
     5221 acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg
     5281 tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg
     5341 taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct
     5401 ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa
     5461 tgcatcaggg ccatctggtc aaagccgccg ttgagctggc aaaagactgg cgtaccgatc
     5521 gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca
     5581 tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg
     5641 gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg
     5701 aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc
     5761 acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac
     5821 gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg
     5881 tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg
     5941 aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg
     6001 cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca
     6061 ccgaagtggg ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg
     6121 ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg
     6181 cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg
     6241 aacagcagca ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc
     6301 agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta
     6361 tggctcctcc aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg
     6421 gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg
     6481 aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg
     6541 ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt
     6601 cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag
     6661 gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt
     6721 ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc
     6781 caatccgcgt tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc
     6841 cgaatgcctc tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg
     6901 agtttaccga ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta
     6961 ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt
     7021 cctacgacgc cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca
     7081 aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt
     7141 caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta
     7201 tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc
     7261 tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact
     7321 tcgatccgct ggtcgccctg ctggtactaa ttaccgccgg gctattacag atcctttcta
     7381 acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc
     7441 cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta
     7501 ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc
     7561 tggccgattt tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct
     7621 acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt
     7681 tctttggctg gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg
     7741 cactgatcct tccggcgacc gcctgtggcc tgctggcaac ggcagtactg aacattaata
     7801 acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag
     7861 gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg
     7921 cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac
     7981 cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac
     8041 caatgctgga acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga
     8101 tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc
     8161 caacagccta catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat
     8221 gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc
     8281 gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg
     8341 tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct
     8401 tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct
     8461 ggcagtacaa aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga
     8521 cctagaagag ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg
     8581 cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg
     8641 cgaccatctt tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga
     8701 atgataagaa aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta
     8761 aagaaattac gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct
     8821 gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct
     8881 cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca
     8941 gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt
     9001 ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga
     9061 tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     9121 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9181 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9241 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9301 ttgcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9361 ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt
     9421 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9481 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt
     9541 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9601 tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9661 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt
     9721 ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt
     9781 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa
     9841 agtgttgatc tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9901 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9961 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
    10021 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
    10081 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc
    10141 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10201 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10261 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10321 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10381 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10441 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10501 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10561 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10621 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10681 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc
    10741 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg
    10801 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10861 tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10921 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10981 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    11041 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    11101 agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga acaccggcga
    11161 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga
    11221 agtgaactat gcgctggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg
    11281 cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca
    11341 aaacaccaat ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc cgtactggga
    11401 cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat
    11461 acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc
    11521 cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt
    11581 cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagttcgcga
    11641 agtgactgca ttgggcgcgg cctatcttgc tggtctggcg gtcggtttct ggcagaacct
    11701 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    11761 tgagcgtaat taccgttacg caggctggaa aaaagcggtt aaacgcgcga tggcgtggga
    11821 agaacacgac gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcccgaa
    11881 acgtgcgggg gcaaccccgc acacatcaat aatccctccc ttcccctgtg ctacacttcg
    11941 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    12001 cgtcaccgaa tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    12061 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca atattgacgg
    12121 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    12181 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    12241 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    12301 cttcctcaat gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    12361 caccattgat ttgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    12421 caaaccgttg agcgaactga cggtaacaat tctggctaaa ccacgccacg atgccgttat
    12481 cgctgaaatg caacaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc
    12541 ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc
    12601 gccggaaggc gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg
    12661 tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    12721 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga
    12781 tatggcgcgc agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct
    12841 ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa
    12901 gtcacgcacc attcgccgca ttcagtctat ccactatctg gatcgcaagg acccggaaat
    12961 gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga
    13021 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    13081 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    13141 caccgttcac gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga
    13201 aatcgacggc gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga
    13261 tctggagttt tacctggtca ccgtccccga tggcaaatta agcccacgac tggcggcact
    13321 gaaaccaggc gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggagga
    13381 agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt
    13441 atcgattctg caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc
    13501 cgcacgttat gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta
    13561 cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac
    13621 cggacggata ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat
    13681 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    13741 acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    13801 gacagcggag cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc
    13861 gcactcatct tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg
    13921 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc
    13981 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    14041 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    14101 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    14161 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    14221 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    14281 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag
    14341 tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg
    14401 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact
    14461 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    14521 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    14581 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    14641 agggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    14701 tgaagagttc agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg
    14761 gaaaaaaata gcagt
//
LOCUS       DAZRUF010000095        14513 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZRUF010000095 DAZRUF010000000
VERSION     DAZRUF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.095"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            93..635
                     /gene="zapC"
                     /locus_tag="ACHXOL_002275"
     CDS             93..635
                     /gene="zapC"
                     /locus_tag="ACHXOL_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR6809247.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(632..1741)
                     /gene="ycbX"
                     /locus_tag="ACHXOL_002276"
     CDS             complement(632..1741)
                     /gene="ycbX"
                     /locus_tag="ACHXOL_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR6809248.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            1985..4093
                     /gene="rlmKL"
                     /locus_tag="ACHXOL_002277"
     CDS             1985..4093
                     /gene="rlmKL"
                     /locus_tag="ACHXOL_002277"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR6809249.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNLGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4105..6012
                     /locus_tag="ACHXOL_002278"
     CDS             4105..6012
                     /locus_tag="ACHXOL_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR6809250.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6142..7395
                     /gene="pqiA"
                     /locus_tag="ACHXOL_002279"
     CDS             6142..7395
                     /gene="pqiA"
                     /locus_tag="ACHXOL_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR6809251.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7400..9040
                     /gene="pqiB"
                     /locus_tag="ACHXOL_002280"
     CDS             7400..9040
                     /gene="pqiB"
                     /locus_tag="ACHXOL_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR6809252.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9037..9600
                     /gene="pqiC"
                     /locus_tag="ACHXOL_002281"
     CDS             9037..9600
                     /gene="pqiC"
                     /locus_tag="ACHXOL_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR6809253.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            9856..10023
                     /gene="rmf"
                     /locus_tag="ACHXOL_002282"
     CDS             9856..10023
                     /gene="rmf"
                     /locus_tag="ACHXOL_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR6809254.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10093..10611)
                     /gene="fabA"
                     /locus_tag="ACHXOL_002283"
     CDS             complement(10093..10611)
                     /gene="fabA"
                     /locus_tag="ACHXOL_002283"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR6809255.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10680..12440)
                     /locus_tag="ACHXOL_002284"
     CDS             complement(10680..12440)
                     /locus_tag="ACHXOL_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR6809256.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12626..13078
                     /gene="matP"
                     /locus_tag="ACHXOL_002285"
     CDS             12626..13078
                     /gene="matP"
                     /locus_tag="ACHXOL_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR6809257.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13154..14194)
                     /gene="ompA"
                     /locus_tag="ACHXOL_002286"
     CDS             complement(13154..14194)
                     /gene="ompA"
                     /locus_tag="ACHXOL_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR6809258.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
BASE COUNT         3613 a         3513 c         3888 g         3499 t
ORIGIN      
        1 atttagacat tttgtacttt tattgttgag gttattaagc gaagcgacaa tggattgtgt
       61 tgctgcggtt ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc
      121 gttggtatta cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat
      181 ttcgctcacg ttttgcgcgc aagatgttga ctccagatgc cttttctccc gcaggctttt
      241 gcgttgacga cgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat
      301 ctaaagaaca aaaagccgaa ctggtgctga atgcactggt agcaattcgc tatctcaaac
      361 cgcaaatgcc gaaaagctgg cattttgttt cccatggtga aatgtgggtg cccatgccgg
      421 gcgatgcggc ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg
      481 aatctggcga aaatgccgca ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc
      541 gcgcgatgca gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtta
      601 atgttgacag cttcagcctc gaacaggcag tctaacgcgc caacttaagt gcagtcttcg
      661 gtacacagct acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg
      721 tgacttcgcc ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat
      781 aagggatacg aattccctga ttttctaatt gctccagcaa cacctgttgg ttatttccac
      841 gaaatgcctg tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg
      901 cggtatcatc agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca
      961 cctcatcgcc aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga
     1021 catcgccgtt atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg
     1081 gatgtttttg ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa
     1141 ccacatcaaa caccacatca ccaatgcgaa tcactttcca gcgatcttct tcccaggctg
     1201 acgcgccgga aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac
     1261 gttgttggag atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa
     1321 atgacagagg tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc
     1381 gtaattgcac ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa
     1441 ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag
     1501 caaaacgaac atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag
     1561 gtgaaggagt aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag
     1621 gttccgtgat cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg
     1681 taagaccaat gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca
     1741 cggtgcggtc ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct
     1801 ataatgcgca acaattttct tagcgcctgt cccattaggc tattttattt gtcagtttgg
     1861 ccctgggttg tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctgtc
     1921 catgtccaga ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga
     1981 cgatatgaat tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga
     2041 actggaaaac ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg
     2101 cgacacacgg cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt
     2161 gccgctgggc gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat
     2221 caactggaca gagatgttta atcttggcgc gaccttcgct gtccacttca gtggtttgaa
     2281 tgacaccatc cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc
     2341 tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt
     2401 taacgtctgg ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt
     2461 acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc
     2521 cgctattgtg atgcgaaccg gctggcagcc aggaacaccg ctgctcgatc cgatgtgtgg
     2581 ttccggtacg ttgctgattg aagcggcgat gctagcgacc gatcgcgcac caggcttgca
     2641 ccgtgggcgt tggggcttta gcggctgggt gcagcatgat gaagctatct ggcaggaagt
     2701 gaaagcggaa gcgcaaactc gtgcccgtaa aggcctggct gagtatagct ctcattttta
     2761 cggttcggac agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg
     2821 gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc
     2881 gaaagggccg tacggcacag tgttgagcaa cccgccatac ggggaacgtc tggacagcga
     2941 accggcgctg attgcgctgc atagcctgct gggtcggatc atgaaaaacc agtttggtgg
     3001 ctggaatctc tctttgttta gtgcctcgcc ggatctgcta agctgcttgc agctgcgtgc
     3061 agacaaacag tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt
     3121 tgccgaaagc actccagaca gcaaaccggc gatggtagcg gaagactacg ccaaccgcct
     3181 gcgtaagaac ctcaaaaaat tcgagaagtg ggctcgccag gaaggaattg aatgttaccg
     3241 cctgtatgac gccgatctac cggaatataa cgttgccgtt gaccgttatg ccgactgggt
     3301 ggtggtgcag gagtatgcgc cgccaaaaac tattgatgct cacaaagcgc gtcagcgtct
     3361 gttcgatatt atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct
     3421 gaaaacccgt gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga
     3481 gtttcttgaa gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga
     3541 tactggtctg ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg
     3601 caaagatttc ctcaacctgt tctcttatac cggtagcgcc accgtgcatg cgggattagg
     3661 cggcgcgcgc agcaccacca ccgtggatat gtcgcgtact tatctggagt gggcagaacg
     3721 caacctgcgt ctgaatggcc tgaccgggcg tgcgcatcgc ctgattcagg ccgattgcct
     3781 ggcgtggctg cgtgaggcaa atgaacagtt tgatctgatc tttatcgatc cgccaacctt
     3841 ctctaactca aaacgaatgg aagatgcgtt tgatgttcag cgcgatcata tggcgctgat
     3901 gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg
     3961 tggcttccgt atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac
     4021 gcaaaaaacg ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat
     4081 taccgcagcc tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg
     4141 ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt
     4201 tgtctggtgg gtcgcaacgg cgcgggcaaa tcgacgttaa tgaaaatcct caaccgtgaa
     4261 caagggctgg atgacggtcg cattatttac gagcaagatt tgattgtagc tcgtctgcaa
     4321 caggatccgc cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa
     4381 gaacaagcgg aatatctgaa acgctatcac gatatttcgc gcctggtgat gaacgacccg
     4441 agcgagaaaa atctcaacga actggcgaag gttcaggaac agttggatca ccacaacctg
     4501 tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaatgtt
     4561 gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg
     4621 agtaatccgc gcgtgctgtt gcttgatgaa ccgacaaacc acctggatat tgaaaccatc
     4681 gactggctgg aagggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac
     4741 cgttcgttta tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg
     4801 acctatccag ggaattacga ccagtacctg cttgaaaaag aagaagccct gcgcgtggaa
     4861 gaactacaaa atgccgagtt cgatcgcaaa ctggcgcagg aagaggtgtg gatccgccag
     4921 gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcaatgcgt
     4981 cgcgagcgtg gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc
     5041 agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt tgacggtaag
     5101 caactggtga aagatttttc ggcccaggtt ctacgtggcg acaaaattgc cctgattggt
     5161 ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcggac
     5221 agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg
     5281 gaactggatc ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg
     5341 gttaacggca agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa
     5401 cgggcgatga cgccggtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg
     5461 cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat
     5521 gtcgaaacgc tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg
     5581 gttagccacg atcgtcagtt tgtcgataac accgttacag aatgttggat cttcgaaggc
     5641 ggcggtaaaa ttggtcgtta tgtcggcggt tatcatgatg cccgtggtca gcaagagcag
     5701 tatgtggcgc tcaaacagcc tgcggtgaaa aaaaccgaag aagccgccgc ggcaaaagca
     5761 gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag
     5821 ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaggtggcg
     5881 gatgcttcct tcttcagtca gccacatgag cagacacaaa aagtgctcgc cgatatggct
     5941 gctgcagagc aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa
     6001 aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg
     6061 cctgatgaac tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata
     6121 gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc
     6181 tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt
     6241 ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tccgaccgcc
     6301 tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg
     6361 aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct
     6421 gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt
     6481 tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag
     6541 caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgct
     6601 ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc
     6661 tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt
     6721 cgctggttat gggacgacat cgccccgatg ccagaactgc gccagccgct aaaaccaggc
     6781 gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct
     6841 gatgaacccg tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg
     6901 cagtggacac tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg
     6961 cccatcatgg tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc
     7021 attctgttat ggagcgaagg ctcttatccc gtcgctgcgg tgatctttct ggccagtatt
     7081 atggtgccaa cgttaaagat gatcgccatc gcgtggctgt gttgggatgc caaagggcat
     7141 ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc
     7201 tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt
     7261 ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca
     7321 atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag
     7381 catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa
     7441 gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct
     7501 tttttatcat tacagccatc agggactgga agtgaccctg atcaccgcga atgcggaagg
     7561 aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag
     7621 cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa gtgcggctga attctggtat
     7681 ggaaaaattg ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga
     7741 agggattagc ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc
     7801 gaaaggcagc aaaatggata aatacgattt gctggactcg ccaccgttgg ccccgcctga
     7861 tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga
     7921 tccggtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca
     7981 aaaacgcaat atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag
     8041 caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtctg cggggatgcg
     8101 tgtggagatg ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga
     8161 aggtctggat ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca
     8221 gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga
     8281 ttcggtacgc ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac
     8341 cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg
     8401 tattccggta ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc
     8461 ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac
     8521 cggaaacctg gtcactggtg cactgtatgt tgatcttgat ttctatccaa atacgcctgc
     8581 aataaccggt attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct
     8641 ggcgcaaatc cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa
     8701 tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct
     8761 gcaaacgacg ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc
     8821 gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg
     8881 ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga
     8941 actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa
     9001 ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt
     9061 gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta
     9121 cctgtggtgc agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta
     9181 gagcaggtca ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat
     9241 gtgaagtatg tgattgccaa caacaacttg tgggccagcc cgttggatca acaattgcgc
     9301 aacaccctgg ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct
     9361 ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat
     9421 ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt
     9481 ccgttccgtc tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtactg
     9541 gccagtgtct ggagtcagga agccgcttct attgcgcaag agataaagcg tctaccttaa
     9601 ttataaagat ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt
     9661 ctaacgtgcg gaaaaagttg ttcctcttta cattttttgt acaaccgaca tacccgtgta
     9721 gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta
     9781 ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta
     9841 tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt
     9901 ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat
     9961 caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc
    10021 tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct
    10081 gtctgattat aatcagaagg cagaagtatc ctggaacaga ccgactttca ggtcgctggc
    10141 ggtatagatc aaacgaccat caaccagcac ttctccatcc gccaggccca taatcagacg
    10201 acggttaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac
    10261 ctgaccagtg aatttcactt cgccaacgcc cagtgcgcgg cctttacctt cgccgcccag
    10321 ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat
    10381 aaccggatcg ccaataaagt ggcatccgaa gaaccacaga tccggattga tatccagttc
    10441 tgcttcaaca taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc
    10501 catcatcagc atattcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgcg
    10561 accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt
    10621 aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat
    10681 cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc
    10741 tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg
    10801 ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag
    10861 atggtgaatt tgccttcttc taccgctttc actagttcac tgtgaagact taaatggcga
    10921 acgttagctg tggggataat gacaccttgt ttcccggtta actcacgttg ctggcaaata
    10981 gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctgggcgcg accgaactga
    11041 tcgactgaac ctgtgatagc gatactctga ttcaccggca catcggcgag ggcgcttatc
    11101 agggcgcaga gttcagccat cgaggcacta tctccatcaa cttcactgta tgactgctca
    11161 aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac
    11221 gcttgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg
    11281 atgtcggtga attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca
    11341 aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt
    11401 tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg
    11461 aggaaacctt cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag
    11521 gtgtcgccat cacacaggga ggcgacctct ttacactggc ggaggatcca ctgcgggcta
    11581 agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccag
    11641 gcatccgctc ccggtgcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac
    11701 cactgggtta ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata
    11761 gcctgctctg aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct
    11821 accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac
    11881 tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac
    11941 agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga
    12001 tgcaccagac caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt
    12061 tgctccgctt ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct
    12121 gcactcactg cgtgacgtaa gcggatggag tggccggaaa cctcatagtg accgcccacc
    12181 agttggcctg catcgctttg tagcgtacgc gcggcattgg caataagatt cagatactca
    12241 gactcttccg gggccttcgc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc
    12301 tccagcgcaa attgcagccg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc
    12361 aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat
    12421 gcaagtttcg taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata
    12481 ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa tactccaggc atgggcgtta
    12541 aagctcacag gatggagatt cttttcttca ctggcctaaa aagctgatat tctgtaaaga
    12601 gttacacggt aacattgaga tcgctatgaa atatcaacaa cttgaaaatc ttgaaagcgg
    12661 ttggaaatgg aagtatctgg tgaagaagca tcgcgaaggg gagttaatca cccgttacat
    12721 agaagccagt gccgcccagg aagccgtcga tgtgttgctc tcgctggaaa atgaacccgt
    12781 gctggtaaat ggctggattg acaagcatat gaatccggaa ctggtcaatc gcatgaagca
    12841 aaccattcgg gcaagacgaa agcgccattt taatgcggaa caccagcata cgcgcaaaaa
    12901 atcgatcgat ctggaattta ttgtctggca acgtctggct ggtcttgcgc agcgtcgcgg
    12961 taaaacgctt tctgaaacga ttgttcagct gattgaagat gcggaaaaca aagagaaata
    13021 cgcgaataaa atgtcttctc tgaagcagga tctgcaggca ttgctgggta aggaataact
    13081 gacgaaagtc agttcaattt actaaaggca aaaaaaaccc cgcagcagcg gggtttttct
    13141 accagacgag aacttaagcc tgcggctgag ttacaacgtc tttgatacct ttaacttcga
    13201 tctctacgcg acgatccgga gccaggcagt cgatcagtgc agcacgctgt ttcacgttgt
    13261 cacaggtgtt gccagtaacc gggttggatt cgcccatacc acgtgcggag atcttgtctg
    13321 ccgggatacc tttggagatc aggtaatcaa caacagactg agcacggcgc tcggacagac
    13381 cctggttgta agcgtcagaa ccgatgcggt cggtgtaacc cagaacaact acggaaccgt
    13441 ctttcggatc caggttgctc agctggctgt acagctgatc cagagcagcc tgaccttccg
    13501 gtttcagggt tgctttgttg aagttgaaca gaacgtcaga cttcagagtg aagtgcttgg
    13561 tctgtacttc cggtgccgga gctggagccg gagcaactac tggagctgct tcgccctgac
    13621 cgaaacggta ggaaaccccc aggctcagca tgccgttgtc cggacgagtg ccgatggtgt
    13681 gtgcgtcacc gatgttgttg gtccactggt attccagacg ggtagcgatt tcaggagtga
    13741 tcgcgtactc aacaccgcca gcgaagaccg gagaaacgcc ggtgtcgtgg tttttaccat
    13801 aaacgttgga tttagtgtct gcacgccata ccataccacc cagacgagtg tagatgtcca
    13861 ggtcgtcagt gattgggtaa cccagtttag cggtcagttg aacgccctga gctttgtatg
    13921 caccgttttc aacgctgcct ttgtacggca tacgacctaa ccagtcgtaa cccatttcaa
    13981 agccaacata cgggttaacc tggtaaccac caaaagcacc agcgcccagt tggttttcat
    14041 gggtcgggcc attgttgttg atgaaaccag tgtcatggta ctgggaccag cccagtttag
    14101 caccagtgta ccaggtgtta tctttcggag cggcctgcgc tacggtagcg aaaccagcca
    14161 gtgccactgc aatcgcgata gctgtctttt tcattttttg cgcctcgtta tcatccaaaa
    14221 tacgccatga atatctccaa cgagataaca cggttaaatc cttcaccggg ggatctgctc
    14281 aattaactct accgatatct tcggcttatg ccgagcaccc ctggcgatgt aaagtctaca
    14341 acgtagttga aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa agtcttgtat
    14401 aaggtatgtt taatcttttt tgtcagcgac aatttacaga agaaaatcgc ggaaaccgct
    14461 tcagacaagc ctccgcaagg aaaattagtc acgactgaaa gcattggctg ggc
//
LOCUS       DAZRUF010000096        14343 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZRUF010000096 DAZRUF010000000
VERSION     DAZRUF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.096"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(188..264)
                     /locus_tag="ACHXOL_002287"
     tRNA            complement(188..264)
                     /locus_tag="ACHXOL_002287"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(228..230),aa:Arg,seq:acg)
     gene            complement(328..404)
                     /locus_tag="ACHXOL_002288"
     tRNA            complement(328..404)
                     /locus_tag="ACHXOL_002288"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(368..370),aa:Arg,seq:acg)
     gene            complement(408..500)
                     /locus_tag="ACHXOL_002289"
     tRNA            complement(408..500)
                     /locus_tag="ACHXOL_002289"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(464..466),aa:Ser,seq:gct)
     gene            complement(816..1001)
                     /gene="csrA"
                     /locus_tag="ACHXOL_002290"
     CDS             complement(816..1001)
                     /gene="csrA"
                     /locus_tag="ACHXOL_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR6809259.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1236..3866)
                     /gene="alaS"
                     /locus_tag="ACHXOL_002291"
     CDS             complement(1236..3866)
                     /gene="alaS"
                     /locus_tag="ACHXOL_002291"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR6809260.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3994..4494)
                     /gene="recX"
                     /locus_tag="ACHXOL_002292"
     CDS             complement(3994..4494)
                     /gene="recX"
                     /locus_tag="ACHXOL_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR6809261.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4722..5783)
                     /gene="recA"
                     /locus_tag="ACHXOL_002293"
     CDS             complement(4722..5783)
                     /gene="recA"
                     /locus_tag="ACHXOL_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR6809262.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5863..6360)
                     /gene="pncC"
                     /locus_tag="ACHXOL_002294"
     CDS             complement(5863..6360)
                     /gene="pncC"
                     /locus_tag="ACHXOL_002294"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR6809263.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6505..7590)
                     /gene="mltB"
                     /locus_tag="ACHXOL_002295"
     CDS             complement(6505..7590)
                     /gene="mltB"
                     /locus_tag="ACHXOL_002295"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR6809264.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7846..8409
                     /gene="srlA"
                     /locus_tag="ACHXOL_002296"
                     /gene_synonym="gutA"
     CDS             7846..8409
                     /gene="srlA"
                     /locus_tag="ACHXOL_002296"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR6809265.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8406..9365
                     /gene="srlE"
                     /locus_tag="ACHXOL_002297"
                     /gene_synonym="gutE"
     CDS             8406..9365
                     /gene="srlE"
                     /locus_tag="ACHXOL_002297"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR6809266.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            9376..9747
                     /gene="srlB"
                     /locus_tag="ACHXOL_002298"
                     /gene_synonym="gutB"
     CDS             9376..9747
                     /gene="srlB"
                     /locus_tag="ACHXOL_002298"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR6809267.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9751..10519
                     /gene="srlD"
                     /locus_tag="ACHXOL_002299"
                     /pseudo
     CDS             9751..10519
                     /gene="srlD"
                     /locus_tag="ACHXOL_002299"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            10625..10984
                     /gene="gutM"
                     /locus_tag="ACHXOL_002300"
     CDS             10625..10984
                     /gene="gutM"
                     /locus_tag="ACHXOL_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR6809268.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11051..11824
                     /gene="srlR"
                     /locus_tag="ACHXOL_002301"
                     /gene_synonym="gutR"
     CDS             11051..11824
                     /gene="srlR"
                     /locus_tag="ACHXOL_002301"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR6809269.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11817..12782
                     /gene="gutQ"
                     /locus_tag="ACHXOL_002302"
     CDS             11817..12782
                     /gene="gutQ"
                     /locus_tag="ACHXOL_002302"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR6809270.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12779..14293)
                     /gene="norR"
                     /locus_tag="ACHXOL_002303"
     CDS             complement(12779..14293)
                     /gene="norR"
                     /locus_tag="ACHXOL_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR6809271.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
BASE COUNT         3341 a         3971 c         3582 g         3449 t
ORIGIN      
        1 ttattctact gctgatattg ataccgctac taaagccata tcaattaaga gatggtgcat
       61 ccgggaggat tactcgcctt gcggctcgcc cttcgggccg ttgctaaagc aacgttatca
      121 tccctggtgc ctgcggttaa ttccgctacc tcttcgatac cttcattgct gaagatttcg
      181 gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt agccgagtac
      241 tctatccagc tgagctacgg atgcatcggg aaaatttatt ttactgctga tattgatacc
      301 gctactaaag ccatatcaag taagagatgg tgcatccggg aggattcgaa cctccgaccg
      361 ctcggttcgt agccgagtac tctatccagc tgagctacgg atgcaaatgg cggtgaggcg
      421 gggattcgaa ccccggatgc agcttttgac cgcatactcc cttagcaggg gagcgccttc
      481 agcctctcgg ccacctcacc acacgcctct tacgagtgct tcgaagaact tgtttatcgc
      541 tcatcgtcgc tgcgtggcgc acatattact ttctgagact tataagtcaa acaatttttc
      601 ccacactttt atcgtttgca cacttcacgc tcaattagtc tgtaaaaacg tcaaaaagag
      661 tgttttatct acagaaaaac ggaggtctgg cagatagcag taatgcaaaa aaatggagac
      721 ttaagttgaa tgaacgggag taaagcgaaa agactataga gtgaaggaga aattttgagg
      781 gtgcgtctca ccgataaaga tgagacgcgg aaagattagt aactggactg ctgggatttt
      841 tcagcctgga tacgctggta gatctcttca cggtgaacag aaacttcctt cggggcattt
      901 acgccaatac gtacctggtt gccctttacc cctaaaactg tcacggtgac ctcatcccca
      961 atcatgaggg tctcaccaac tcgacgagtc agaatcagca ttctttgctc cttgaaagat
     1021 taaaagagtc gggtctctct gtatcccggc attatccatc atataacgcc aaaaagtaag
     1081 caatgacaaa cacattacat gtaagcagtc atggcattac attctgttaa acctaagttt
     1141 agccgatata cacaacttca acctgacttt atcgttgtcg atagcgttaa tgcgaatgcc
     1201 gtgaagcgag tccacggcat tgcctgacgc ttatattatt gcaatttcgc gctgacccag
     1261 cctttcacac tggctaacgc tgcaggtaag gccgcagcat ccgtaccacc ggcttgcgcc
     1321 atgtcaggac gtccaccacc cttgccgccc acctgctgag cgaccatacc aatcagttcc
     1381 cctgctttca cacgatctgt gacgtcctta gatacgcctg caatcagaga aaccttacct
     1441 tcggctaccg ttgccagcac gataattgtc gaccccagct gattttttaa atcgtcaacc
     1501 atggtacgca acattttcgg ctcaacaccg ctaagctcgc taaccaacag cttaacacca
     1561 ttaacatcaa ttgccttact ggaaagattt gcgctctcct gtgcggcagc ttgttcttta
     1621 agctgttgta gttctttttc taactgacgc gtacgttcca gtactgagcg cactttatcc
     1681 gccagattat tgctatcgcc tttcagcaga tgcgcgactt cgcttaagcg atcgctgtct
     1741 gcatgaacgg tggtgatagc gccttctccg gttaccgctt cgatacgacg aacgcctgca
     1801 gcagtacccg attcagagat gatgcggaac agaccaatat caccagtgcg gctggcgtga
     1861 gtaccgccac acagctcggt ggagaaatcg cccatgctca gcacgcgtac acgctcatca
     1921 tacttctcgc cgaacagcgc catcgcacct ttcgctttcg ccgcttcgag atccatgatg
     1981 ttggtttcga tcggcaaatt gcgacgaatc tgtgcgttca ccaggtcttc gaccgcacga
     2041 atctcttccg gtttcatcgc ttcgttgtgt gagaagtcga agcgcagcac cttgtcgtta
     2101 accagtgaac ctttctgcga tacatgagtc cccagaacct ggcgcagcgc agcgtgcatc
     2161 aggtgcgttg cggagtgatt cagacgaata cgggcgcgac gagcctcatc aacatcagcc
     2221 tgcacagcgt cgcccacttt cagagaaccc gcagcaagtt taccgatgtg accaatcgcc
     2281 tggccgtatt tctgcgtatc ttccaccgcg aaggagaagt tagcgccttt cagttcgcct
     2341 ttatcaccaa cctgaccgcc ggattccgca tagaatggcg tttgatccag cacgaccaca
     2401 gcttcctggc ctgcattgat ggcatcaacc gctttaccat caacaaacag cgcggtcact
     2461 ttgccgttca gttccagatg gtcatagcct ttaaattcag atgcactatc aacacggatc
     2521 attgcgttgt aatcggcacc aaagccgctg gcttcgcgcg cgcgacgacg ctgctcttcc
     2581 attgctgctt caaaaccagc ttcgtcaact ttgatgttgc gctcacgaca aacatcagcc
     2641 gtcaggtcaa ccgggaagcc ataggtgtcg tacagacgga aagccgtttc accatccagc
     2701 gtatcaccag aaagttttgc cagctcttca tccagcaacg ccagaccgcg ctccagagta
     2761 cgggcaaact gctcttcttc agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc
     2821 aggtcttcac ccgcagagcc cataacgtcg atcagcggac caaccagttt gtagaagaag
     2881 gtttctttcg cgccgagcat attgccgtga cgcactgcgc gacgaatgat acgacgcagt
     2941 acataaccac ggttttcatt ggacggcatt acgccatccg cgatcaggaa cgcacaagaa
     3001 cgaatgtggt cagcgattac gcgcagcgat ttattgctca gatcggtcgc gccagtgact
     3061 ttcgctaccg cctggatcaa cgtgcggaac aggtcgatgt catagttaga gttaacgtgt
     3121 tgcagcaccg cagcaatacg ctccagaccc ataccggtat ctacagacgg cttcggcagc
     3181 ggttccatcg tgccatctgc ctggcggttg aactgcatga agacgatgtt ccagatctca
     3241 atgtagcggt cgccgtcttc ttccggactt cccggagggc caccccaaat gtggtcgccg
     3301 tgatcgtaga agatttcggt gcacgggccg cacggaccag tgtcacccat ctgccagaag
     3361 ttgtcagatg cgtatggcgc acctttgtta tcgccgatgc gaataatacg ttcgcgcggg
     3421 atccctactt ctttttccca gatttcgtag gcttcgtcgt cgctttcata gacggtaacc
     3481 cacagacgct ctttcggcag ggcaaaccat ttttcgctgg tcagcagttc ccatgcaaac
     3541 tgaatggcat cgtgtttgaa atagtcgccg aagctgaagt tgcccagcat ttcgaagaag
     3601 gtatggtgac gcgcggtgta accgacgttt tccaggtcgt tgtgtttacc acccgcacgc
     3661 acgcagcgtt gggaagtggt agcgcgggaa taattacgct tgtcgagccc aaggaacaca
     3721 tccttgaact ggttcatccc ggcgttggta aacaacaaag ttgggtcgtt atggggtacc
     3781 agggagctgc tggcaactac ctgatgtccc ttactatgga aaaagtcgag aaacgcctga
     3841 cggatctcag cggtgctctt gctcataatt atcctgaaat caagctaacg aaatatcgcc
     3901 accagctcca gcgtgtctta accgccgggc tggtaactga aaagtgggaa taagataagt
     3961 tttcttgagt gggaagtaaa ataccgtatg cgttcagtcg gcaaaatttc gccaaatatc
     4021 ctggatatct tccatcagat agccacgata gagcagaaaa cgctggatct taactttttc
     4081 tgaaaagaca gttggcaaag gttcaccata ttttcgcgtc gcctgatcgc gcaccagtgc
     4141 gcaccagtcg atgtcacatt cacgcatcgc tttttctgtc gcttcgcggg aaataccttt
     4201 ctgattcagt tcctgacgaa tacgtgcagg tccataacct ttgcggctac ggctggcgat
     4261 aaagcgcgca acaaatcggc tgtcatcgag atagccatgt tcatggcacc aggcaataac
     4321 gcgctcgtaa tcttctgccg tagcatcaat ctcttctggg ccatttttgc ccataatcgg
     4381 tgccgcgagt ttacgtcgca gttcttgctc actgtgatcg cgcaccgcca gaatgcgtac
     4441 cgcacgatcc aacaggcgag catatgccgg gcgacgggat gttgattctg tcatggcata
     4501 tccttacaac ttaaaaaagc aaaagggccg cagatgcgac ccaggttttg ctgataaaca
     4561 gatttaatag tcaatgagtg tgcagccaat tcgttagagg atcgaggtga gagtaatttt
     4621 tcacgccagt gttggcctgt tcccctcacc ctaaccctct ccccaaaggg gcgaggggac
     4681 cgtccgtgca cacattatct attatcatca ataacgaacg attaaaaatc ttcgttagtt
     4741 tctgctacgc cttcgctgtc atctacagag aaatccggcg ttgagttcgg gttgctcagc
     4801 agcaactcac gtactttctt ctcgatctct ttcgcggttt ccgggttatc tttcagccag
     4861 gcagttgcat tcgctttacc ctgaccgatc ttctcacctt tgtagctgta ccacgcgcct
     4921 gctttctcga tcagcttctc ttttacgccc aggtcaacca gttcgccgta gaagttgata
     4981 ccttcgccgt agaggatctg aaattcagcc tgtttaaacg gtgcagcgat tttgttcttc
     5041 accactttca cgcgggtttc gctacccacc acgttttcgc cctctttcac cgcgccgata
     5101 cgacggatgt cgagacgaac agaggcgtag aatttcagcg cgttaccacc ggtagtggtt
     5161 tccgggttac cgaacatcac accaattttc atacggatct ggttgatgaa gatcagcagc
     5221 gtgttggact gcttcaggtt acccgccagc ttacgcatcg cctggctcat catacgtgcc
     5281 gcaaggccca tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg cgtcagtgcc
     5341 gccacggagt caacgacgat aacgtctact gcgccagaac gcgccagggc gtcacagatt
     5401 tccagtgcct gctcgccggt gtccggctgg gagcacagca ggttatcgat atcgacgccc
     5461 agtttacgtg cgtagattgg gtccagcgcg tgttcagcat cgataaacgc acaggtttta
     5521 ccttcacgct gcgctgcggc gatcacctgc agcgtcagcg tggttttacc ggaagattcc
     5581 ggtccgtaga tttcgacgat acggcccatc ggcagaccac ctgccccaag cgcgatatcc
     5641 agtgaaagcg aaccggtaga gatggtttcc acatccatgg aacggtcttc acccaggcgc
     5701 atgatggagc ctttaccaaa ttgtttctca atctggccca gtgctgccgc caacgctttc
     5761 tgtttgtttt cgtcgatagc catttttact cctgtcatgc cgggtaatac cggatagtca
     5821 atatgttctg ttgaagcaat tatactgtat actcatacag tatcaagtgt tttgtagaaa
     5881 ttgttgccac aaggtctgca atgcatacgc agtagcctga cgacgcaccg catcacggtc
     5941 gccgctgaag cattcccgcc gggtaatgcc ttcaccgcgg gcagtggcaa aagcaaacca
     6001 gacggtgccg acaggcttct cttcactgcc gccatccggc ccggcgatac cactaataga
     6061 cacggcataa tcagcacgag ccgctttcag tgcacctatc gccatttcca ccacgacggg
     6121 ttcactcacc gcgccatgct gcgccagcgt ctcttcgcgt acgccgatca tctgcgcttt
     6181 ggcttcgtta ctgtaggtga caaatccgcg ttcaaaccag gcggagctac cggcaatatc
     6241 ggtaatcact ttcgctaccc aaccaccggt acaagactcg gcagttgtta cggttgcgcc
     6301 acgggctttc agcgcctgcc caacctgttc gcttaactgc atcagttcac tgtcagtcat
     6361 cacaacctca gtcagtcaaa aatttatgcc ggacaagata gcactttcgt caggaagatg
     6421 gtgttggcgt tgcaatttta cgaggaggat tcagaaaaaa gctgattagc cagagggaag
     6481 ctcacgcccc cctcttgcaa atagttactg cacgcgcgcc agcgccacgg cttgtcctaa
     6541 ctgccagacc gccattgcgt aatgggtgct gtggttataa cgggtaatgg tgtagaagtt
     6601 cggcagaccg taccagtact ggtaaccggt accgacatcc agacgcagca ggctggcttg
     6661 ttgatggttg cccagcggct gctgtggcgt taaacctgcc gtggcaagct gcgaaatgct
     6721 gtacctggtt ttgaagccat ttggcaagcc tggagcctga ccgtttgcca ttaccgcgac
     6781 ctgatcgcct ttcacccagc cgtgcgcttt gaaatagttc gccacactac cgatcgcatc
     6841 aaccggatcc cacaggttga tatgcccgtc gccgctgaaa tctaccgcat attgtttgta
     6901 agacgacggc ataaactgtc cgtagcccat cgccccggca aaggaacctt tcagattgag
     6961 cggatcgtcc tgctcgtcgc gcgccatcaa caggaaggtt tccagctcgc cagagaaata
     7021 ctccgcgcgg cgtgggtagt taaatgacag cgttgccagc gcatcgagga tgcgagtttt
     7081 ccccatcacg cgcccccagc gggtttcaac gccgataatc ccgacgataa tttccggcgg
     7141 tacgccatac acctgccacg cgcgattcaa cgcatcttca tactgattcc agaaaaccac
     7201 accgttctgc acgttgtccg gcgtaataaa ttttttgcga taacgcagcc atgcgccgtt
     7261 cggacctgat ggcggtttca ccgatgtggt tggtgcctgg ttatccatca gccgcagtac
     7321 cgaatccaga cgcttcgcct gggagagaat ttcctgcaac tgctgacgat cgaaaccgtg
     7381 tttgttcacc attctgtcga tgaactgctg ggcattcggg ttattagcga aatcgccgcc
     7441 catctgcatc acattgtgct gaggctcaag caggaagccg ccagacggcg ttccggtggt
     7501 cgtttcagtc tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca caaaaagggg
     7561 aagcaatgtt acataacgac gcttgaacat gaggggtcca tttaacagat tcaaccaggg
     7621 gcaagtatgg taaagcatca cgccccgcac aaggaagcgg tagtcactgc ccgatacgga
     7681 ctttacataa ctcaactcat tcccctcgct atccttttat tcaaactttc aaattaaaat
     7741 atttatcttt cattttgcga tcaaaataac acttttaaat ctttcaatct gattagatta
     7801 ggttgccgtt tggtaataaa acaataaatc ctgaaggaga gaacaatgat agaaaccatt
     7861 actcatggtg cagagtggtt tatcgggctg ttccaaaagg gcggagaggt gtttaccggg
     7921 atggtgaccg gcattcttcc gctgttgatt agcctgctgg ttatcatgaa cgcactgatt
     7981 aattttatcg gtcagcatcg tattgaacgt tttgctcaac gttgcgccgg taaccctgtt
     8041 tcccgttacc tgctgctacc gtgcattggc acgtttgtct tttgcaatcc gatgacctta
     8101 agcctgggtc gctttatgcc ggaaaagtac aaacccagct actacgcggc agcctcttat
     8161 agctgccact caatgaatgg cctctttccc catatcaacc ctggcgaact gtttgtttat
     8221 cttggcattg ccagcggtct gacaacgctg aacctgccac ttggcccact ggcggtgagc
     8281 tatctgctgg ttggtctggt caccaacttc tttcgcggct gggtgaccga tctgaccacc
     8341 gccatttttg agaaaaagat gggcattcaa cttgaacaaa aagttcacct ggcaggagca
     8401 acatcatgac gcgtattcgg atcgaaaaag gaacgggtgg ctggggcggc ccgcttgagc
     8461 tggaagccac gccgggcaaa aaaatcgtct atatcaccgc cggtacccgg cctgcgattg
     8521 ttgacaaact ggcacaactt actggctggc aggctattga cggatttaaa gaaggtgaac
     8581 ccgcggaggc ggaaattggt gtcgcggtaa tcgactgtgg cggcacatta cgctgcggca
     8641 tctatccgaa acgacgtatt cccaccatta atatccactc gacgggcaag tccagtccgc
     8701 tggcgcagta cattgtggaa gatatttatg tctctggcgt aaaagaagaa aacatcactg
     8761 tagtgggtga tgcgacacca caaccctctt ccgtgggccg tgactatgac accagcaaga
     8821 aaatcaccga acaaagcgat ggtttactgg cgaaggtggg aatgggtatg ggttctgccg
     8881 ttgccgtgtt gtttcaatct ggtcgtgaca ccatcgacac tgtattaaaa accattctgc
     8941 cgtttatggc gttcgtctcg gcgctcattg gcatcattat ggcttctggc cttggtgact
     9001 ggattgccca cggtcttgct ccgctggcga gccatccact gggtctggtc atgctggcgc
     9061 tcatctgctc cttcccgctg ctttcacctt tcctcggccc aggcgcagtt atcgcacagg
     9121 ttatcggcgt attgattggc gtgcagattg gtctcggcaa tattccgccg catctggctt
     9181 taccggcact gtttgccatc aacgcgcagg cggcctgcga cttcatcccg gtcggtttgt
     9241 cgctggcgga agcccgtcag gacacggttc gcgtcggtgt cccttctgta ctggtgagcc
     9301 actttttaac cggcgcaccg actgtactga tcgcctggtt tgtctccggt tttatctatc
     9361 aatagaggct gaaacatgac cgttatttat cagaccacca tcacccgtat cggcgcgagt
     9421 gccactgacg ccctcagcga ccagatgctc atcacctttc gtgaaggcgc gccagcggac
     9481 ctcgaagagt attgcttcat tcattgccac ggcgagttga aaggtgcact ccatcccggt
     9541 ttgcaattta cgctcgggca gcatcgctat ccggtgaccg ctgttggcag cgtggcggaa
     9601 gacaaccttc gcgaactggg tcatgtcacc ctgcgcttcg atggtttaaa cgaagcggaa
     9661 tttccgggca ctgtccatgt ggcaggccct gtacccgacg atatcgcgcc gggatcggtt
     9721 ttgaagtttg aatctgttaa ggagtaaaaa atgaatcagg ttgccgttgt catcggtggt
     9781 gggcaaacct taggcgcgtt cctgtgccac ggtctggctg ccgaggggta tcgcgtcgcg
     9841 gttgtcgata ttcagagcga caaagccgca aatgtggcac aagaaattaa cgccgaatat
     9901 ggtgaaggaa cagcgtatag ttttggtgct gacgccacca gcgagcaaag cgtcctggcg
     9961 ctctctcgtg gggtagatga aatctttggt cgcgtggatt tgctggtcta cagcgccgga
    10021 atagcaaaag cagcctttat cagcgacttc cagctcggcg attttgaccg tgaatctggt
    10081 gggttatttc ctgtgtgccc gtgaattttc tcgtttgatg atccgcgacg ggattcaggg
    10141 tcgcattatt cagatcaact cgaaatccgg caaagtgggc agcaaacaca actctggcta
    10201 cagcgcagcg aaatttggtg gcgtcgggct gactcaatca ctggcgctgg atctggcgga
    10261 gtacggcatt acggtgcatt cactgatgct cggtaacctg ctgaaatcgc ctatgttcca
    10321 gtcactgtta ccccaatacg cgaccaagct gggtatcaaa ccggatcaag tcgagcagta
    10381 ttacatcgac aaagtaccgc tcaaacgcgg ctgcgattat caggatgtgc tgaatatgct
    10441 gctgctctat gccagtccta aggcgtcgta ctgcaccgga cagtcgatca atgtcaccgg
    10501 cggtcaggtg atgttctaat caacagcgga gatccgttaa ggatctccgt gagactaata
    10561 gaatgcctga tgcgctacgc tcatcaggca tacaggactt ccgccactac attaaggaaa
    10621 agttatggta tccgcactca tcaccgtcgc cgttatcgcc tggtgtgcgc aactggcctt
    10681 aggcggctgg caaatttctc gttttaaccg tgccttcgac acactatgcc agcaagggcg
    10741 ggttggcgtg ggccgttcca gcgggcgctt taaaccacgg gtcgtggtcg ccatcgccct
    10801 ggacgatcag cagcgcatcg tcgacacctt gtttatgaaa ggactgaccg tcttcgcacg
    10861 accgcaaaaa attcccgcaa ttaccggtat gcatgcgggt gatttacagc ccgatgtgat
    10921 ctttccccat gatccactat cacagaatgc tctatcattg gcgcttaaac tgaaacgtgg
    10981 ataatttcgt tgtgaatgtt acttgcttgc gaagttatca ttttgaaacc taaatcaggt
    11041 aatcacgccc atgaaacctc gtcagcgtca ggccgccatt ctggagtatc tgcaaaagca
    11101 gggtaaatgc tcggttgaag aattggcgca atactttgac accacaggca caaccattcg
    11161 caaagatctg gtcattctgg aacatgcagg aaccgtcatt cgtacttatg gcggagtggt
    11221 gttgaataaa gaggaatccg atccgcctat cgatcataaa acactcatca acacccacaa
    11281 gaaagagctg atagcagaag ctgccgttag ttttattcat gatggcgatt cgatcattct
    11341 tgatgctggc agtaccgttt tgcagatggt tcccctgctc tcgcgcttta ataacatcac
    11401 ggtgatgacc aacagtctgc atatcgtcaa tgcgctatct gaactggata acgaacaaac
    11461 tatcctgatg ccaggcggaa cgtttcgtaa aaaatcggcc tcatttcacg gacagctggc
    11521 agagaatgcc ttcgaacatt tcagtttcga taaattgttt atgggcaccg acggcatcga
    11581 tctcaatgcg ggcgtaacca cctttaacga ggtttatacc gtcagtaagg caatgtgcaa
    11641 tgccgcgcgc gaagtgattt tgatggcgga ctcatcaaag tttggccgta aaagccccaa
    11701 cgtagtttgc agtcttgaaa gcgtcgataa gctgattacc gacgcaggta tcgatccggc
    11761 gtttcgtcag gcgctggaag agaaagggat cgatgtgatc ataaccggag agagcaatga
    11821 gtgaagcact actgaacgcg ggacgtcaga cgttaatgct ggagttgcag gaagcaagcc
    11881 gtttaccgga acgtctgggc gatgattttg ttcgcgccgc caatatcatc ctgcactgcg
    11941 aaggcaaagt ggtggtttcg ggaattggca aatcgggcca cattggtaag aaaatcgccg
    12001 caacacttgc cagtaccggc acatcggctt tttttgtcca tccggcagaa gcactgcacg
    12061 gcgatctggg gatgattgaa agccgcgatg tgatgctgtt tatctcttac tccggtggcg
    12121 cgaaggaact ggatctgatt attccgcgtc tggaagataa atctatcgcg ctgctggcga
    12181 tgaccggcaa accgacgtca ccgctgggcc tggcggcaaa agcggtgctg gatatctccg
    12241 tagaacgcga agcctgcccg atgcaccttg cgccgacctc cagcaccgtc aataccctga
    12301 tgatgggtga cgcgctggcg atggcggtca tgcaggcgcg cggatttaat gaagaagatt
    12361 ttgcccgctc ccacccagcc ggggcactgg gcgctcgctt gctgaataaa gtgcatcatc
    12421 tgatgcgccg tgacgatgcc atcccacagg tggcgttaac cgccagcgtg atggatgcga
    12481 tgctggaact cagccgcacc ggtctggggc tggtggcggt atgtgacgct caacaacagg
    12541 tacaaggcgt ctttaccgac ggcgatttac gtcgctggct ggtaggcggc ggcgcactca
    12601 ccacgccagt taatgaagcg atgacgacag gcggcaccac gttacaggcg caaagtcgcg
    12661 ccatcgacgc caaagagatc ctgatgaagc gcaaaatcac tgccgcaccg gtggtggatg
    12721 aaaacggcaa actcaccggc gcaattaacc tgcaggattt ctatcaggcc gggattattt
    12781 aatccttcat tcccagacgt ttcgccagcc gatgcaggtt ggcgacgtcg gtttccagca
    12841 tccgcgcgca ggcagcccag ttatgatgat tttgtgccag tgcctggcga atagtttcac
    12901 gctggaacgc ttctgtcgct tcacgcaggt tttgcttaac aacgggcacc gccgccgctt
    12961 ctggcggcgg caacgtcacc tcaggaaaag caaaatgttg cgcctcaaga atcacttcat
    13021 cgccgttgcg ggtggctctc gccagcacta ccgcccgatg aatagcatgt tccagttcgc
    13081 gcacgttccc cggaaaacga tagtgttgca gtaaatttcg cgctccggca cttaatacca
    13141 cgcgggagag ccccagccgc aaacgacact gctcgcagaa ataccccgcc agcagaatga
    13201 catcatcgcc ccgctcacgc agcggcggca ccgaaagtgg aaacacgctc aggcgatgaa
    13261 acaagtcagc gcgaaatcgc cctgccatca cctcttcgcg taagtcgcgg ttagtcgccg
    13321 ccagcacgcg cacatcgacc cgcaaactac ggtcatcgcc aacgcgctga atatcgccat
    13381 actgcaacac cctcagcagc ttggcctgca atgccaacga caactcgccg atctcatcca
    13441 gaaacagagt gccgttatcc gccatttcaa acttcccgct gcggttactg atagcgccag
    13501 taaacgctcc tttcacatgc ccgaacaact cactttccgc cacactttcc ggcagtgcag
    13561 cacagttgag atagaccagc ggattcaccg cccgtggcga ggcttcatga atcgctttcg
    13621 ccaccagctc cttaccggtt cccgtctcac cgctgatcag gacgttgaga tcggacgccg
    13681 ccacaatctc aatctctttt ttcagttgcg tcatgccagg ggacaagcca atcatctgcg
    13741 tctgtttcac cgcttcaaac ggcgtggcat cgcctggcat catattctgg ctttccagtt
    13801 gttcaatcag caacgcattg cttaacgctc ccgccgccag cgcagcaatc agccgtagct
    13861 cttcgtcgct gaaaacatcg aactgatcgg gctgcatccc gtcgagcgtc agtgcgccga
    13921 tcaggttttg cccggcaaac aatggcagac caacgcaggc gtgaaccttc agactctcct
    13981 gcccaggaat caaaccgtca tagggatcgg gcaattcgct gtctgcggga aagcgcacca
    14041 catccccggc gcgggcaatc gcttccagcc gtggatgccc ttccagcgca aagcgtctac
    14101 cgagtacatc ctttgccaga ccgtcgatgg caagcggaat aaactgccgc gaatcgtaac
    14161 gtagcaacgc agacgcatcg cactccagca cctgacgtag cgtggtgatc aggcgctgaa
    14221 aacgatcctg atgaccaatc ccacgctgca attcgatggc gatattcgcc agcacatcaa
    14281 cggaaaaact catctttgcc tcactgtcaa tttgactata gatattgtca tatcgaccat
    14341 ttg
//
LOCUS       DAZRUF010000097        14080 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZRUF010000097 DAZRUF010000000
VERSION     DAZRUF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.097"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHXOL_002304"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHXOL_002304"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR6809272.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="ACHXOL_002305"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="ACHXOL_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR6809273.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHXOL_002306"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHXOL_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR6809274.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHXOL_002307"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHXOL_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR6809275.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHXOL_002308"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHXOL_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR6809276.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="ACHXOL_002309"
     CDS             5280..5738
                     /locus_tag="ACHXOL_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR6809277.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="ACHXOL_002310"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="ACHXOL_002310"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR6809278.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="ACHXOL_002311"
     tRNA            6790..6874
                     /locus_tag="ACHXOL_002311"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="ACHXOL_002312"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="ACHXOL_002312"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHXOL_002313"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHXOL_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR6809279.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHXOL_002314"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHXOL_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR6809280.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHXOL_002315"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHXOL_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR6809281.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="ACHXOL_002316"
     CDS             complement(10156..13899)
                     /locus_tag="ACHXOL_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR6809282.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3483 a         3731 c         3370 g         3496 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcaatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac
//
LOCUS       DAZRUF010000098        13972 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZRUF010000098 DAZRUF010000000
VERSION     DAZRUF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13972
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.098"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="ACHXOL_002317"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="ACHXOL_002317"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR6809283.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="ACHXOL_002318"
     CDS             2273..2662
                     /locus_tag="ACHXOL_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR6809284.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="ACHXOL_002319"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="ACHXOL_002319"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR6809285.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHXOL_002320"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHXOL_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR6809286.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="ACHXOL_002321"
     CDS             complement(4321..4521)
                     /locus_tag="ACHXOL_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR6809287.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="ACHXOL_002322"
     CDS             4687..5232
                     /locus_tag="ACHXOL_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR6809288.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="ACHXOL_002323"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="ACHXOL_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHXOL_002324"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHXOL_002324"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR6809289.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="ACHXOL_002325"
     CDS             complement(6529..7353)
                     /locus_tag="ACHXOL_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR6809290.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHXOL_002326"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHXOL_002326"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR6809291.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHXOL_002327"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHXOL_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR6809292.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHXOL_002328"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHXOL_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR6809293.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHXOL_002329"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHXOL_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR6809294.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /locus_tag="ACHXOL_002330"
     CDS             complement(11316..11969)
                     /locus_tag="ACHXOL_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR6809295.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHXOL_002331"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHXOL_002331"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR6809296.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHXOL_002332"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHXOL_002332"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR6809297.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3390 a         3540 c         3715 g         3327 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagacggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagaccccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gc
//
LOCUS       DAZRUF010000099        13906 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZRUF010000099 DAZRUF010000000
VERSION     DAZRUF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13906
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.099"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(1..869)
                     /gene="yhaJ"
                     /locus_tag="ACHXOL_002333"
                     /pseudo
     CDS             complement(1..869)
                     /gene="yhaJ"
                     /locus_tag="ACHXOL_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            974..1675
                     /locus_tag="ACHXOL_002334"
     CDS             974..1675
                     /locus_tag="ACHXOL_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR6809298.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1698..1862
                     /gene="yhaL"
                     /locus_tag="ACHXOL_002335"
     CDS             1698..1862
                     /gene="yhaL"
                     /locus_tag="ACHXOL_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR6809299.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1996..3306)
                     /gene="cyuA"
                     /locus_tag="ACHXOL_002336"
     CDS             complement(1996..3306)
                     /gene="cyuA"
                     /locus_tag="ACHXOL_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR6809300.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3334..4665)
                     /locus_tag="ACHXOL_002337"
     CDS             complement(3334..4665)
                     /locus_tag="ACHXOL_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR6809301.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGAFYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4940..6304)
                     /gene="tdcG"
                     /locus_tag="ACHXOL_002338"
     CDS             complement(4940..6304)
                     /gene="tdcG"
                     /locus_tag="ACHXOL_002338"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR6809302.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6376..6765)
                     /locus_tag="ACHXOL_002339"
     CDS             complement(6376..6765)
                     /locus_tag="ACHXOL_002339"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR6809303.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6779..9073)
                     /gene="tdcE"
                     /locus_tag="ACHXOL_002340"
     CDS             complement(6779..9073)
                     /gene="tdcE"
                     /locus_tag="ACHXOL_002340"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR6809304.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9107..10315)
                     /gene="tdcD"
                     /locus_tag="ACHXOL_002341"
     CDS             complement(9107..10315)
                     /gene="tdcD"
                     /locus_tag="ACHXOL_002341"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR6809305.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10341..11672)
                     /gene="tdcC"
                     /locus_tag="ACHXOL_002342"
     CDS             complement(10341..11672)
                     /gene="tdcC"
                     /locus_tag="ACHXOL_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR6809306.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11694..12683)
                     /gene="tdcB"
                     /locus_tag="ACHXOL_002343"
     CDS             complement(11694..12683)
                     /gene="tdcB"
                     /locus_tag="ACHXOL_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR6809307.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12782..13720)
                     /gene="tdcA"
                     /locus_tag="ACHXOL_002344"
     CDS             complement(12782..13720)
                     /gene="tdcA"
                     /locus_tag="ACHXOL_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR6809308.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3621 a         3755 c         3252 g         3278 t
ORIGIN      
        1 aatttcccgc agacaccagg atttcgcttc ccccatactg tcacgacgcc aggccataat
       61 aatatcgatc tcactggtcg attccgggct gacgacacgc aaccgccctt ccgcaatatc
      121 tttttcgacc atcggatacg gcatcgtcgc cacgccaagc cccgccagta atgcctgacg
      181 tttatcttca atcgtgctca ccgttaagcg cggctgtttg tccagcagtt gtacggtcaa
      241 caccgggcgc tcacgagcgg tatccgctac cgcaattcca cgatatttca cgcgcgtcac
      301 ttcagataac ggttccggct cctgatgaat cgggtgatca ggcgcggcaa cgtagacgtt
      361 cattaacgta tagagcttgc gcgagttgat ctccgacgag gaacgaaaat gcatatccgg
      421 cgcgataaca atatccgccc gcccctgctc cagtcgttcc tacgcccccg ccagcacttc
      481 ggtgatgatt gccagttggg tattggcttt tgccgccagt ttgtcgatta acgggaaaaa
      541 ggcaggtgtc ggtaccagcg cttcggtcac aatggtgaga tgcgtttccc aaccgcgcgc
      601 gagggcttcc gcatcggtag tcagtttatc tgcggcttcc agcaaaacgc gccctcgctc
      661 cagcaacatc ctcccgacat tggtgaattt ggtgcgatgg cccgagcggt caaacagcac
      721 cacatccagc tcttcttcca gtttttgcat ggtgtagcta agtgcggaag gcacgcgtcc
      781 cagctcatcc gccgccgccg caaaactgcc ccggcgatcg catccataac ccgtagtgct
      841 tccagcgtta atgccctttc tttggccatt tcgttctcat tcaggaaatt tgaacatacc
      901 gggcagaata tctggctaac aatgtagcgt ccagcccctt accataaaag gaagtaaaga
      961 gaggtcaaga attatgatta ctacccgaac tgccaggcag tgtggacaag cagactacgg
     1021 atggttgcag gcccggtata ctttttcctt tggacactac ttcgacccga aattgttagg
     1081 ctatgcctcc ctgcgtgtgc ttaaccagga agtgctggcc ccaggtgccg cctttcagcc
     1141 gcgaacctat cccaaagtcg atattttaaa tgtgattctg gatggagaag cagagtatcg
     1201 cgacagcgaa ggcaatcatg ttcaggccag cgccggtgag gcgttgctgc tctctaccca
     1261 gccgggtgtc agctacagcg aacacaatct cagcaaagac aaaccgttaa cacgaatgca
     1321 gctttggctg gacgcctgcc cgcagcgaga gaatccgctg attcaaaaac tggcgcttaa
     1381 tatgggcaag cagcaattaa tcgcctcgcc agagggtacg atgggaagcc tgcaattacg
     1441 ccagcaagtg tggctgcacc atatcgtgct cgacaaaggc gaaagtgcga atttccagtt
     1501 gcatgggcca cgcgcgtatt tgcaatcgat tcacgggaaa tttcatgcgc ttacgcatca
     1561 tgaagagaaa gcggcgctga cctgcggtga tggggcgttt attcgtgacg aggctaacat
     1621 tacgctggtt gccgattccc cactgcgcgc tttgctgata gatttgcctg tctagttgtt
     1681 tttacaggga gatgatgatg agtaaaaaat cggccaaaaa gcgccagcca gtgaagcccg
     1741 tggtggcgaa agaacctgct cgcaccgcca aaaattttgg ctatgaagag atgttgagcg
     1801 agctggaagc catcgtcgcg gatgctgaaa cgcgtttagc cgaggatgaa gctaccgcgt
     1861 aaagacaggt tgccggatgc ggcgaaaacg ccttatccgg cctacaaaat ccaacaacat
     1921 taacgaatta aacaacccgt aggccggata aggtgcgcca gcatcgcatc cggcacaata
     1981 ctcaaaacct ggcgtttatc tggccttgct cgccataatc tcgataatct gccgatccgt
     2041 ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct caacatcatg
     2101 cgccacgatc ccttcattgc cggtcacggc ggtatcatcc agcgccatta acaccgcttt
     2161 ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg caccatcgca
     2221 aatcatgccg ctgacatcgc cgatcatact gctgatcgcc atcgagatag tttcataacg
     2281 cccatccacc agccatgcca tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca
     2341 aagtgcagac aaacgcggta actggttatg gatgtaaatt gcgctcaaat gagaaagcat
     2401 cagcgcacgc gccagtcgtt catcatccgc tccgaagtgt tctgctacaa ccaccacagg
     2461 cattgttgcg gtgatcccct ggttacccga gccggagtta ctcatagccg gaagcgtagc
     2521 gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg aagagagatc
     2581 tttcgccagc aaaccgcgct cgcactgttt ttccagcgtc gcgccaatat gcagccccca
     2641 cttaccgctc aaaccttcct gcgataacgc acaatttagc ttcgcggaat cgagaataaa
     2701 gcggatcgcc gcaaacggga cttcattgac gaacttcagg atctcagcca gcgtcgttct
     2761 ggaaagcacc gtcagcggag actcttgctc gccctctgcc acacacgcct gctgggtaaa
     2821 caccacacca ttgtgcgtct caatatgcac aatgttggta tgcccgccga cgatggtgac
     2881 acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt cattgcaagg
     2941 ttcctggatc ttaacggaga ctttccccgc cgccagcagt gctttggcat cggcaattgc
     3001 ctgcgcagtt gcgtctttca gcacttccag cccggcgttg gcatttccac ctaacgcccc
     3061 cagcgccgcc gcaatcggca gccccaccat tcccgtgccg ggaacggtga cgcccagacc
     3121 gttcttcatc agatttggcg aaacccaggc ttctacacgt tcaaccggac cttccagttc
     3181 tgccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacatc ccagcgccgg
     3241 ttttacttcc tcctgaacgg cgaggatgta acgctgccat aacggattta aagtcgaatc
     3301 aaacatatta aaaaccttaa aatttcaggt aaatcaagaa aatgccagga acggagaaac
     3361 acaaagcaac aaaccagtga cgataatcag gtacagagac atccctttgt atttgtgcaa
     3421 tgccggtact ttgtaaacca gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg
     3481 gctacagata gaggtgaagc tcaacaccgg tgcgttcagt acgatagcgc tccaggccag
     3541 caaaatggcg aaaatcatga tgccgcgctg aacaagattt tcgttaatct tctcggcagg
     3601 catcttgcga cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa agcctaaata
     3661 gacgccaaag aatgcagtca tgacagcaaa gatattgaga atgacgctga ccactttcac
     3721 ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg aaatattctg
     3781 ctcgtaggct tttaccgctt cgtcatgccc catcgccagc gtgaacgaca cggcgtagaa
     3841 aaagacggtg acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg ctttatgccg
     3901 cgccacttca atagattttt cccgcgagcg ataagagatc accatcgggc ttaacgtctg
     3961 gataaacaaa atcgacgtca gggtaaacgg cagcgtaata atggcgtttt tcaccagcag
     4021 ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca tcgacacgcc
     4081 cagcgccgcg accaccagca gcttggtcag caccatgccg gtcgaaattt tgaataacaa
     4141 tttctcgccg cgtgaagaga tcgccaccag aatgcaaatc agtaccagac cataaaaggg
     4201 actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc agataggaag cactatcgtt
     4261 ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat agaacgcacc
     4321 taacaggatg ccccagtttt tacctaaata accgctaatg acactcgggt aatctttaca
     4381 ttctggtgat tctgccagcg tattaataaa caaccgctga aacagataca ttgccgggta
     4441 accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct gcaccgggag
     4501 aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc cggtgtcagt
     4561 actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac gacccgccgg
     4621 ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt ctcgctcaat
     4681 attttgtagg gcttattatt tttttccgag ccgcatcaag gcgatatgcg gtctacgttt
     4741 ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa aatcttcaca
     4801 atcaagactt tgaaataacg gagatggata agaattttct actaaattaa tcgcaagaga
     4861 atattcattc tctatttgcg gcagatcaca aaaaaaaggt gcacatttgt gcacccaagg
     4921 atgaaagctg acagcaatgt cagccgcaga ccactttaat ggccagtcct ccgcgtgatg
     4981 tttcgcggta tttatcgttc atatctttgc cggtttcata catcgtctcg atcactttat
     5041 cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat ccacgcggcg tttactgctt
     5101 tcacggcatt aatggcatta cgttcaatgc acgggatttg tacctgtccg gcaaccggat
     5161 cgcaggtcag cccaaggtta tgctccatcg cgatttccgc cgcattgcat acctgcgccg
     5221 gactgccgcc cagtagttca gttaaccctg ccgccgccat tgaacaggcc acgccaatct
     5281 ccccctgaca gccgacctcc gcgccagaga tggaggcgtt cattttatac agcgcgccaa
     5341 tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc gtttaccgga cgacggaact
     5401 tatcgtaata agccagcact gccggaataa tgccgcacgc accgttagtc ggtgccgtta
     5461 ccacgcgccc gccagctgcg ttttcttcac taaccgccag cgcgtacatg ttgatccagt
     5521 cgatgacatt catcggatcg ttagagatgt tatcgctaga aaccagctga cgacgcagcg
     5581 ctacggcacg acgcggcaca ttgagcggac caggcagcac gccttcagtg ttcatcccgc
     5641 gttcaatacc gtcatgcatc acttgccaga tacgggcaaa tcctgcgtca atttccgctt
     5701 tgctgcgcag cgccagctcg ttgtgcatca tcaggccaga tatagacagg ccgttgtaat
     5761 cacacatttt cagcagttca cctgctgagt ggaaatcgta aggtacggac gtttcgacat
     5821 cgtgcgacag gccgaagtgt tcttcttcga caataaaccc gccgccgaca gagtaatagg
     5881 ttttacttaa tagcgcttcc tgtcccttcc aggcagtgat ccgcattccg ttctcatggc
     5941 gaggcaacat ttcgggatgg aagataatgt tctttgctac aggaaaatca acaatatgcg
     6001 caccagatgc cactggcagc cgcccgctgc gcgttactaa ctctataaat gcagggatct
     6061 catcaatgac aacatcctgc ggactgtttc ctgccagtcc catgataatg gcgacatccg
     6121 tggcatggcc tttgcccgtc agtgacaacg acccatacag atcgaccaca atatggctcg
     6181 tcgcggttaa taagccgcta ctttccagcc gatcaataaa actttttccg gcattcattg
     6241 gccccacggt atgcgaactg gagggaccaa tcccaatttt gaaaatatcg aatgcactaa
     6301 tcatatccac accctcggat tgccgttcag tgaagtggag cggaacgacc ttgcgaccgt
     6361 cccgctcacg aggctttacg cactacgtac tgcgatagct tcaatttcca gcttcacatc
     6421 tttcggcaaa cgcgcaacct gcacacagct ccgggtcggg taggtcgcct gatgctcatc
     6481 gaagaactgc ttatagactt cattgatggt ggcaaaatca ttcagatcgg tgatgaacac
     6541 ggtcatcttg atgatatcgc ccacgctcag cccggcagca accacgatcg ctttgacgtt
     6601 ttcgaggctt aaacgcgcct gatcttgcac atcagccggg atctcaccgg tctgcgggca
     6661 aaccggaatt tgcccggagg tgaagaccat gctgcctaaa tcaacgccct gaacataagg
     6721 gccgattgcg cctggggcac gttgcgtttc gataatcttt ttcatacatc ctccggcgtc
     6781 agagcgcctg ggtaaaagta cgtgaaataa catcctgttg ctgttcacgg gtcagtgcgt
     6841 tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt aggatatttt tccgggtgct
     6901 cgatggcatc cagcagcatt tcccgattca ttacgttgac gttgaggtgt tgaccgcctt
     6961 cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc gacaaggttg gttttacgta
     7021 ctggatcttc tttgcccagc gccgcaggaa cgattgagaa ggtgtacgag atcccatctt
     7081 tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga ggccacggca cctttgcggt
     7141 cacgaccatg catcgggtta gcgcccggcg cgaacggtgt tccggcgcga cgaccgtccg
     7201 gcgtattacc ggttttctgg ccgtacacca cgttagaagt gatagtcaga atcgactggg
     7261 tagggacggc gttgcgatag gttggcagcg ctttaatttt cttcataaag cgttcaacca
     7321 ggtcgcaggc aatgctgtct acgcgctcgt cgttgttgcc gtactgcgga tattcaccgt
     7381 cgatttcaaa gtccaccgcc aggccgtttt cgtcacggat tggtttcacg cgggcatatt
     7441 tgatggcaga cagggagtcc gtcgccaccg acaggcccgc gatgccgcat gccatagtgc
     7501 gatagacatc acgatcgtgc agcgccatca gcgaagcttc gtagctgtac ttgtcgtgca
     7561 tgtagtgaat gatattcagc gcgctgatgt actgcaccgc cagccagtcc atgaagtgat
     7621 cgaggctgtc catcactttg tcgtaatcca gcacgtcgtc catcagcggt gctgttttcg
     7681 gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt aattgcgtac agcagtgttt
     7741 tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt accaatcacc attgggctga
     7801 cgcaacaagc aattgcgtaa tcgtcgctgt tgaagtcagt acgcatcaga tcgtcgtttt
     7861 catactgcaa ggaagaggtg acgatcgaca cctgcgcggc atattttttg aaggcaatcg
     7921 gtaattcttc cgaccaaaga atggtcaggt taggttccgg tgccggcccc atagtgtgca
     7981 gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt acgaccgtcc agccccatcc
     8041 cgccgatcac ttccgtcgcc cagattgggt cgccggagaa cagcgaatca aattccggcg
     8101 tacgcaagaa gcgcaccata cggatcttca taatgaagtg atcgatcagt tcctgtgcct
     8161 gctgctcatt gagtacgcct gctttaaagt cgcgctcaat gtagatatcg aggaacgacg
     8221 ccgtgcgccc cagcgacatc gcgccgccgt tctgtgattt cactgccgcc agataagcga
     8281 agtagagcca ctgcaccgct tcctgcgcat tctgcgccgg gcgagagata tcaaagccat
     8341 atttcgccgc catttcctga atctgcaaca gcgcatgacg atgctctgcc agctcctcac
     8401 gcagacggat ggtggcttcc agatcttcgc ctttttccag acgggactgg agatcggcaa
     8461 attgcagttc gcgttcacgc accagataac tgatgccata cagcgctacg cggcgatagt
     8521 caccgataat gcgcccacgg ccatagccat ctggtaaacc ggtcagcacg ccagatttac
     8581 ggcagcgcag catatccggt gagtaaacat caaatacgcc ctggttatgg gttttacgca
     8641 gatcggtaaa cagatattca aattcactgt ccatttcacg gccataggcg tggaatgaac
     8701 ttttaatcat attaatgcca ccgaacggat gcagcgcgcg ctttaatggc gcatccgttt
     8761 gcagaccaac aattttttcc agcggctggt taatatatcc cgcatcatga gcggtaattg
     8821 tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt tgcgttttcg atacggatgc
     8881 cttccattac tttttcccac aattccgtgg tggcaggcgt cgcttcggcg aggaaagatt
     8941 catcgccttc atacggtgta tagttatgtt gaataaaatc gcggacatta atttcgtttt
     9001 tccagtccgt acctttaaag ccaagccatg cgtcggcgta cagcttatcg ctggtatcaa
     9061 tatctatctt catgaaaaat aatctctcta caatacttca actaaattat gcaaattctg
     9121 cgggcgcgtt aactttgcct aaatgaatgg catccaaagc aatcattttt tcttcgttag
     9181 tcggaataac ggcacaaatg acacgcgcat tttcactgga aacaattcgc tctccaaaag
     9241 agttagagcg attattcatt tctgtatcaa tcactacgcc taatacagcc aaatgttcca
     9301 tgaccagacg acgaattaaa cttgaattct ctcctattcc gccggtgaat ataattccat
     9361 ccaggcgacg taatgaagct gcgtgtccgg caatatgacg ggcaattcgg tgaacaaagg
     9421 ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca ggctttttcc agaacacgta
     9481 aatccgaaga aagaccggaa atacctaata atcccgactc tttattcact acgcgttcca
     9541 ggtcacccag gctctggttg gtttggctgg cgacccagga catcgcacca aagtcgacat
     9601 cgccactgcg ggttcccatc atcaagcctt ccagcggcgt cattcccatt gaagtatcaa
     9661 cactctgacc gttgcgaacc gcgcagattg acgcgccatt gccaagatgc gccacaacca
     9721 ggccggagtc atcttccgcc agattcagca gagaatgtgc gcgctgggaa acatagcggt
     9781 gcgacgtgcc gtggaaacca taacggcgta cacctaactc ttcataatat ttccacggca
     9841 ggccgtataa ataagcttcc ggagccatcg tctggtggaa actggtatca aataccgcca
     9901 cctgagttac gcccggaaat aattgctgcg ccgattcaat accacttaaa ttggcgtaat
     9961 tatgcagggg tgccagtgga gaaacgcgac ggatattatc aatgacttca tcggtaataa
    10021 tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg gtggccaatt aaggccacac
    10081 tgtcatttaa attccgtttt tccagttcaa atgcaattgc cttcaatgca ccttcgtagc
    10141 tgtggtgagc cagcggtgct ggctctcccc catttacgga taagaatgca ttttccgagt
    10201 taataccgtc ggcaatacct gacattaata cttcacagtc gctggcatcg agcacggaaa
    10261 acttaatcga agacgaacca cagttaataa ccaaaacaac cggaaattca ttcatctctt
    10321 ttctcatcct gagttacgga ttaaaacagt ttgtatacga tgttcaggat ggtcagcaga
    10381 ccaatcacgg taacaaacac gttatccaga cgaccacggt atttcgccag agacggcgct
    10441 ttacggatgg catacatcgg caacaggcac agcagggatg cgataatcgg tgcgcccatg
    10501 gcttcaatca ggtcgaggat gttcgggttg gcgtaggcaa caacccaggt ggagcccatg
    10561 atgaagatca tgctgatagt gttcagttta cccagcgaca ctttagtttt gtcgccttta
    10621 taaccaaact tcaggaccag gccattcaga ccttccagcg ttcccagata gtgaccgaag
    10681 aaagatttga agatagccac gagtgcgatg atggaagccg catattccag tgtaatcgcg
    10741 aacgttgttt tggtaccggt catggacgca aagtggttag ccagataaga aagcactgga
    10801 atattctgcg ctttggcttc cgccatgttg gccggagaca gcgtaaatag gcagctaaag
    10861 gcaaagaaca tcaccactgc aaccatcagc atgctggcac gagaaatgat ttgggaacat
    10921 ttacgttcgg tgaagtcgcg accgaagtct ttctcatact cttcacgttt agaaaccacg
    10981 aaggaagaga cgattggcga gaaattaaag gagaaaacca tgatggaaat ccccagccag
    11041 acggtgatca ggataccgtc atgaccggtt aacgacagcg aaccgaggtc aacctggtcg
    11101 ataactgcag agttccagta agggatcagc gacagagaaa tcagcaccag gctggcgata
    11161 aacggccata ccaggtagct catcacttta accatcagat ccttaccaaa ccagatgacg
    11221 aaagccatca gcagcagcag gaacagcgcc acaaagccgc gattcagcgg tgcaaagccg
    11281 agctggtttt cccagaacgt cataaaggta ttggtaatag taacgccata aatccacagc
    11341 agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca cgccgccagt tttaccaaaa
    11401 tgctcttcca ccgtttccgt aatgttgccg gaagggttag agccagaaag acatagacgc
    11461 gccagcgccc ggtggcaata aaatgcgatg gggtatgcca ataccaacat cagaagaatc
    11521 gggatcagtc cgccaaaacc tgcgcggata gggaagaaca gcaccccggc gccgattgcc
    11581 gtaccaaaca agcctaacgt ccatgtggta tctgatttac gccaggacga ttgttttgtc
    11641 tggctggata caatgctatc tgaagtactc atatcctatc ctcaacgaat taattaagcg
    11701 tcaacgaaac cggtgatttg agagacgcga gaaagatcga tattgccgcc ggaaataata
    11761 ctgacggttt ttctgttttg aatatattgg tctaatttac cgcttaataa tgcagcacat
    11821 gccagagcgc ctgcgccttc ggtgacgact ttattgcgct gaattaaggc aatcatactg
    11881 tttctgattt cgtcttcgct gaccagcacg atgtcatcga ctaattcacg aacgatttcg
    11941 taagttaaat tacccgggcg ggagacatca caaccatccg ccagggtgcc ggtagttcgg
    12001 tgcgtggtta tttctccgga gtggaaagaa gccgccatgc cgtgaacgtt ttcagactgt
    12061 acgccaataa cacgaatggt cgggttaata gatttaattg ccaccgcaat accagcaatt
    12121 aaaccgccac caccaattgg cacaatcacg ttatcgacat catagagatc ttccataatt
    12181 tccagaccaa tcgttccctg gccagcaatc actttcggat catcgtaagg tgggataaaa
    12241 atacggcctt ccatttcgac aatttcgctc actttagcga tagtgtcgtt gaagttatca
    12301 ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg ctactttgga ttttggcgca
    12361 ccttttggca tcaccacttt accgtcgata cccagcatcg cgcaggagag cgaaacccct
    12421 tgcgcatggt tgcccgcaga acaggccacc acgcctttgc gtttttccgc atcggtcagt
    12481 gaacttaatt tattaaatgc gccacgaatt ttaaatgaac ccgtacgctg catattttca
    12541 aacttcagga atatttcacc tttgcaacgt tcactaaaat agttggagcg aggcatgcct
    12601 gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa taatatcatc aatagcaatc
    12661 ggcagatcgt atgtaatatg cattataaaa cctcttcgcc tgaattaaat atgtaggtaa
    12721 ccgtaaccga cacctgcaaa acagacaggt ggattattta tgtttaaata aaaacaaata
    12781 actaaccaac ttctattaat tgccttcgtc tacacccatt ataagatgaa tactctttgg
    12841 ctaattccac caaaaccgat gctgcttttt taatacgata atttttcgac cataccgcgg
    12901 catattgtgc cacaggtaat gtttcttcaa ccggaatagt aataaattga ttagaaccaa
    12961 aaggtgacgt catatcacaa ggaattacag ttaagaaatc agcattgaga acaagattat
    13021 aaattgtcac gactgagtcg gttttaacga tgttttcaat actgatgcca tttctttgta
    13081 acgtagtaag cagttcgctg tagtacccca tattagtttg tggcaacacc cactgttcgt
    13141 tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg ggacttactg gctaccagca
    13201 caaactcgga ctcgaacagc ggctcaacat gtaaatcctg aagcttcatt tctgcactta
    13261 acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc cggtaagaac gaagacagtt
    13321 gcgcttcata catagaaacc tgcgctttcg ggaacacctc tttgaacttg ttgatcatcc
    13381 ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa tgagacttcc accaccgcct
    13441 cagaagacat accgcttatc tcattaacca tatttttcat ttcacgggta atggattcgg
    13501 aacgggagag taacaattga ccggcaggtg ttaatgttac accggtattt ttccgcacca
    13561 ctaattccac accaaaataa tcttcaatat cgttaatgat tttactgacg gccggttgag
    13621 ttaaccctaa ttcttttgca gccgagccga tagagccact tctaatgact tcctgaaaga
    13681 ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat aacgacctac gttaattacc
    13741 tcattgacgg catgaagtgt atcaaaatga aatgaacagg atatgtgcga ccactcacaa
    13801 attaactttc aatactttcc agggtatcgt cattacaatt aaataccttt aaatatcaac
    13861 aagttaaagc ataaaaatca gcataaaccc tgatttttgt caaaaa
//
LOCUS       DAZRUF010000100        13853 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZRUF010000100 DAZRUF010000000
VERSION     DAZRUF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.100"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /gene="dsbG"
                     /locus_tag="ACHXOL_002345"
                     /pseudo
     CDS             complement(1..736)
                     /gene="dsbG"
                     /locus_tag="ACHXOL_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
     gene            1108..1671
                     /gene="ahpC"
                     /locus_tag="ACHXOL_002346"
     CDS             1108..1671
                     /gene="ahpC"
                     /locus_tag="ACHXOL_002346"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR6809309.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1842..3407
                     /gene="ahpF"
                     /locus_tag="ACHXOL_002347"
     CDS             1842..3407
                     /gene="ahpF"
                     /locus_tag="ACHXOL_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR6809310.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="ACHXOL_002348"
     CDS             complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="ACHXOL_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR6809311.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4177..5415
                     /locus_tag="ACHXOL_002349"
     CDS             4177..5415
                     /locus_tag="ACHXOL_002349"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFR6809312.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5419..5508)
                     /gene="yldA"
                     /locus_tag="ACHXOL_002350"
     CDS             complement(5419..5508)
                     /gene="yldA"
                     /locus_tag="ACHXOL_002350"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR6809313.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="ACHXOL_002351"
     CDS             complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="ACHXOL_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR6809314.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6286..7092)
                     /gene="rna"
                     /locus_tag="ACHXOL_002352"
     CDS             complement(6286..7092)
                     /gene="rna"
                     /locus_tag="ACHXOL_002352"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR6809315.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            complement(7206..8669)
                     /gene="citT"
                     /locus_tag="ACHXOL_002353"
     CDS             complement(7206..8669)
                     /gene="citT"
                     /locus_tag="ACHXOL_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR6809316.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHQEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8720..9598)
                     /gene="citG"
                     /locus_tag="ACHXOL_002354"
     CDS             complement(8720..9598)
                     /gene="citG"
                     /locus_tag="ACHXOL_002354"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR6809317.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNFASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9573..10124)
                     /gene="citX"
                     /locus_tag="ACHXOL_002355"
     CDS             complement(9573..10124)
                     /gene="citX"
                     /locus_tag="ACHXOL_002355"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR6809318.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10128..11660)
                     /gene="citF"
                     /locus_tag="ACHXOL_002356"
     CDS             complement(10128..11660)
                     /gene="citF"
                     /locus_tag="ACHXOL_002356"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR6809319.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11671..12579)
                     /gene="citE"
                     /locus_tag="ACHXOL_002357"
     CDS             complement(11671..12579)
                     /gene="citE"
                     /locus_tag="ACHXOL_002357"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR6809320.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12576..12872)
                     /gene="citD"
                     /locus_tag="ACHXOL_002358"
     CDS             complement(12576..12872)
                     /gene="citD"
                     /locus_tag="ACHXOL_002358"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR6809321.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="ACHXOL_002359"
     CDS             complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="ACHXOL_002359"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR6809322.1"
                     /translation="VDTTVEVFITVTRDEKLITCGGIAGNIIKCVAISESVRGEGLAL
                     TLATELINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRY
                     AESLKKFRHPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYED
                     RLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLA
                     PALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISAS
                     RVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3400 a         3506 c         3688 g         3259 t
ORIGIN      
        1 aatgatatta agcgttttct gatcgggcaa ccccacggcc tgttgcagcg tattttcctt
       61 actcatgtaa tagatagccg gcgtgacatt tgcccccaga tcgtccatca gtttctcatt
      121 gtcacttaac actttcattt gctctgtact tacgtttgca ggcacgttta gcttaagctt
      181 gccaccagag gcttcatatt gttgccaggt ttttgcggga tctttggagg caagaattgc
      241 cgctgctgtc gccgggcttt ctggcttgat aaccccaacc aacaatgttc ttaattgcac
      301 tttgccagaa tctacccacg ggcgcgcctg ctgccagaac tgtttacaat atgggcagaa
      361 cggatcggcg aagacgtaga caatgaccgg cgcatctttt ttaccgtcga ggatccagtg
      421 ggattgttcc atccgttgcc acatttcgcg tccggctggt gcgtaaattt ctttttcgat
      481 aagtgtgtta ctcaggtttt cacctttctc gttgtacatg taaccagaga tagcgtgctt
      541 accatcggga gtcaggtagg tagatggtga cgcccatatc ctgatacttt ccgagataac
      601 ctttcattcc tccgggggca tcgaacgttt tgatgattgt aatgccctgt ttttcaatcg
      661 cttttactgg ggaaggaagt tcctctgcga aggcgattgc aggaagcaga gccagtaaaa
      721 gtatcttttt taacattaat ttgtcctttt cagtcagtgc aaaagtcgag taaaaggcat
      781 aacctatcac tgtcataggt aagagcttag atcaggtgat tgccctttgt ttatgagggt
      841 gttgtaatcc atgtcgttgt tgcatttgta agggcaacac ctcagcctgc aggcaggcgc
      901 tgaagatacc aaagggtagt tcagattaca cggtcacctg gaaagggggc cattttactt
      961 tttatcgccg ctggcggtgc aaagttcaca aagttgtctt acgaaggttg taaggtaaaa
     1021 cttatcgatt tgataatgga aacgcattag ccgaatcggc aaaaattggt taccttacat
     1081 ctcatcgaaa acacggagga agtatagatg tccttgatta acaccaaaat taaacctttt
     1141 aaaaaccagg cattcaaaaa cggcgaattc atcgaaatca ccgaaaaaga taccgaaggc
     1201 cgctggagcg tcttcttctt ctacccggct gactttactt tcgtatgccc gaccgaactg
     1261 ggtgacgttg ctgaccacta cgaagaactg cagaaactgg gcgtagacgt atacgcagta
     1321 tctaccgata ctcacttcac ccacaaagca tggcacagca gctctgaaac catcgctaaa
     1381 atcaaatatg cgatgatcgg cgacccgact ggcgccctga cccgtaactt cgacaacatg
     1441 cgtgaagatg aaggtctggc tgaccgtgcg accttcgttg ttgacccgca gggtatcatc
     1501 caggcaatcg aagttaccgc tgaaggcatt ggccgtgacg cgtctgatct gctgcgtaaa
     1561 atcaaagcag ctcagtacgt agcttctcac ccaggtgaag tttgcccggc taaatggaaa
     1621 gaaggtgaag caactctggc tccgtctctg gacctggttg gtaaaatcta aatttccttc
     1681 gtctttcgcg ctaccaactc cggcgcgaaa tacttcggaa attcacctaa ttcttcgggt
     1741 gctgcggcac ccgattttct tccccgcacc atgatgcaag ctgcatccag gtagccgtag
     1801 aggccgcttg catgatgatg tttaaagccc aggagataaa catgctcgac acaaatatga
     1861 aaactcaact caaggcttac cttgagaaat tgaccaagcc tgttgagtta attgccacgc
     1921 tggatgacag cgctaaatcg gcagaaatca aggaactgtt ggctgaaatc gcagaactgt
     1981 cagacaaagt cacctttaaa gaagataaca gcttgccggt gcgtaagccg tctttcctga
     2041 tcaccaaccc aggttccaac caggggccac gttttgcagg ctccccgctg ggccacgagt
     2101 tcacctcgct ggtactggcg ttgctgtgga ccggtggtca tccgtcgaaa gaagcgcagt
     2161 ctctgctgga gcagattcgc catattgacg gtgattttga attcgaaacc tattactcgc
     2221 tctcttgcca taactgcccg gacgtggtgc aggcgctgaa cctgatgagc gtactgaacc
     2281 cgcgcatcaa gcacactgca attgacggcg gcaccttcca gaacgaaatc accgatcgca
     2341 acgtgatggg cgttccggca gtgttcgtaa acgggaaaga gtttggtcag ggccgcatga
     2401 cgttgactga aatcgttgcc aaaattgata cgggcgcgga aaaacgtgcg gcagaagagc
     2461 tgaacaagcg tgatgcttat gacgtattaa tcgtcggttc cggcccggcg ggtgcagcgg
     2521 cagcaattta ctccgcacgt aaaggcatcc gtaccggtct gatgggcgaa cgttttggtg
     2581 gtcagatcct cgataccgtt gatatcgaaa actacatttc tgtaccgaag actgaagggc
     2641 agaagctggc aggcgcactg aaagttcacg ttgatgaata cgacgttgat gtgatcgaca
     2701 gccagagcgc cagcaaactg atcccagcag cagttgaagg tggtctgcat cagattgaaa
     2761 cagcttctgg cgcggtactg aaagcacgca gcattatcgt ggcgaccggt gcaaaatggc
     2821 gcaacatgaa cgttccgggc gaagatcagt atcgcaccaa aggcgtgacc tactgcccgc
     2881 actgcgacgg cccgctgttt aaaggtaaac gcgtagcggt tatcggcggc ggtaactccg
     2941 gcgtggaagc ggcaattgac ctggcgggta tcgttgagca cgtaacgctg ctggaatttg
     3001 cgccagaaat gaaagccgac caggttctgc aggacaaact gcgcagcctg aaaaacgtcg
     3061 acattattct gaatgcgcaa accacggaag tgaaaggcga cggcagcaaa gtcgttggtc
     3121 tggaatatcg agatcgtgtc agcggcgata ttcacaacat cgaactggcc ggtattttcg
     3181 tgcagattgg tctgctgccg aacaccaact ggctcgaagg cgcagtcgaa cgtaaccgca
     3241 tgggtgagat tatcattgat gcgaaatgcg aaaccaacgt caaaggcgta ttcgcagcgg
     3301 gtgactgtac gacggttccg tacaagcaga tcatcatcgc cactggcgaa ggtgccaaag
     3361 cctctttgag tgcttttgac tacctgattc gcaccaaaac tgcataagaa gaagtaagat
     3421 tcacctgcaa ttgcttagcc gccggggtca aacctggcgg cttttttatg gcattaaaaa
     3481 gccctgccgg gatgttccag gcagggcggc ataaatactt ttatctgtta gcgaatgacc
     3541 agtacaggca gattggcgtg gcggatcacg ctcgaggcgt tagaacctaa cagatgggtc
     3601 gaaatcgatg ggttgcgaga accgataact acaacatcag cccccagttc ttctgccaac
     3661 tcattgactt catcccgcac gctaccaaaa cggacatgtt gtttaatgcg ggaaggatcg
     3721 atggtgaagt ggctgaccat cgtttgcaga cgttcttctg cttcatgttg cagatgctct
     3781 tcaaaacgac gcacatcagc ggcaaaacgg tgcaggctca ggctggctga cccgggtagt
     3841 acgtgaagta gatgaataac tccgtcatcc tgggcgagga attcagcgtg gcgaacagct
     3901 ttgtcgctca attccatttc aaatacatca actggcataa tgattgtctt atacataacc
     3961 ctttctccct gttaatcatg aacaaatcat tcgccatgat tataatattt atccctgata
     4021 tttgtctggt tctttttcct tacgaacggt ttctgtgatt aatatattct cactgaacac
     4081 caggaattct cccaaaacct gcggtaccgc ccgttttccc gctgtgatag ctacccttaa
     4141 agactgactc ttttttgaac tgtctctgga ggttgcatga aagcattgac ttatcacggc
     4201 ccacatcacg ttcaggtaga aaatgttccc gatccgggca ttgaacaggc tgatgatatt
     4261 attctgcgta ttacggcaac ggcgatctgt ggctctgacc tccatcttta tcgaggcaaa
     4321 ataccccagg ttaaacatgg cgatattttg ggtcatgaat ttatggggga agtcgttgaa
     4381 accggaaagg acgtaaaaaa tttgcaaaaa ggcgaccggg tggtaattcc gtttgtcatt
     4441 gcttgtggcg actgtttttt ctgtcgaatg cagcaatatg ccgcctgcga aaataccaat
     4501 gcgggtaaag gcgctgcgct caataaaaaa cagataccag ctcccgcggc attgtttggt
     4561 tatagtcacc tgtatggtgg cgttcctggt ggacaggcgg aatatgtccg cgtccctaaa
     4621 gggaatgtgg ggccgtttaa agtaccgcct ttgctttcag atgataaagc ccttttcctt
     4681 tctgatattc tgccaacggc atggcaggca gcaaaaaatg cgcagatcca acaaggttca
     4741 agcgttgcag tctatggtgc cggtccagtg ggattgttga caatcgcctg tgcacggttg
     4801 ctcggtgcgg aacagatttt tgttgttgat catcatccct accgcttgcg tttcgccgct
     4861 gaccgctacg gcgcgatccc gattaatttc gatgaagata gcgatccggc acagtcaatt
     4921 attgaacaaa cggcaggtca ccggggcgtg gatgcagtaa tagacgccgt cggttttgaa
     4981 gcgaaaggca gcaccacgga aacggtgctg actaacctga aactggaggg cagcagcggt
     5041 aaagcgttgc gccagtgtat tgcggcggtc aggcgtggcg gcattgttag cgtaccgggc
     5101 gtctacgctg gatttattca cggtttcctg tttggcgacg cctttgataa agggttgacg
     5161 tttaaaatgg gacagaccca cgttcacgca tggctgggtg aattattacc gttaattgag
     5221 aaaggattac tgaaaccaga agaaattgtt acccactata tgccgtttga agaggccgcc
     5281 cggggatatg agattttcga aaaacgtgaa gaggagtgcc gtaaggtgat tctggtaccc
     5341 ggtgcacaaa gcgcagaggc ggcgcagaag gcggtttcag gtctggtgaa tgcgatgccg
     5401 gggggaacaa tatgatcgtc aggagtggtt ttcgaggtaa aggacagcca tgacgataat
     5461 cgccgccata atcagaaatc ctatcaggat gtaaaatgct tctgccatgg ttattcccac
     5521 aaacgaaacg gaataatttt gcagcaaaag tgaacagtga gaaccaggaa aaattgctga
     5581 ttttgcgtaa agaggatgcg agtgcatcct ctgggcaaag cgagttatcg cttgtgcaga
     5641 tgggattaaa gcaggtagtc gccagcagct tctggctggt attcgagttc cagcacttca
     5701 aggtgggttg caacgccgcc cggaagttcc cagtgaatgg aatcgccaac gcgcagcccc
     5761 agcagtgcgg cacctaccgg agccataacg gaaagctgag tattgctatc ggtcattttt
     5821 gccggataca ccagcgtgcg cacacgcact tcgccatcgc taagattgcg gaatttaacc
     5881 cggctgttca ttgtcaccac gtcgtgtggc atctcttctg gcgaacacat ttgggcgcga
     5941 tccaactctg cgtttaacgc gtcggcgatt ggcaaaccag cataggcggg ttgctccagc
     6001 agaatatcga tgcgttcggc atccaggtcg ttaatgatga tagttggtct ggacattttt
     6061 actccatgtc gtcggtactg cgagtgtcgc agataaacat acccaaaaga aaaccctcac
     6121 cgtcaggcgg cgagggttta actcacatga tgatactgac tgttgctcac tctttgaagt
     6181 gatttgcgtc acattcaggg aattcctcaa tgcagcgcat tatgtataat tcttaatcgt
     6241 cacggtttat ggaagacgaa ttagcgtgtt ttgtaaatca gatgattaat aacccacttt
     6301 atcaatcaca aaggttttgc cacagttgcc tgggtgtggt tgtggcaaga atgagtttgc
     6361 agaaagcgga gcgttgatgg cgtcagcttt gatcgagatc tgaatttcag tcaaatacgc
     6421 agggttaccc tggcacgtta gcttaactgc tttcacgttc tcttttcccc agcttttggc
     6481 aaaggcggcg tcaaagtcac ggcggctcac tgttttaccg taattatccg caaggaattt
     6541 tccggcttcg ctctctttaa tttcttgatt caggcgtacc atcgtaccga agtatgcgtc
     6601 cggatcaaaa ccaaagcagg caccgtgttt ggcatattcg tagcgttcca ggcaggaacg
     6661 tccgccagct cctggcatga cttcacttag tttagcggcc gtttccagtg ataacccggt
     6721 ttccggcgat gaacacatcc ggctggcgtg cgcttctggt agattcggga ttgggcgagt
     6781 agcgcaaccg aagcgcatcc agcggcgttc atcaacacca cgggcagcaa ccgatttagg
     6841 caatcctggc cacagaccat gtacggtcag aaaatcagct ttgttggtcg tttcggtttg
     6901 taggcgacat tcatctcgtt cgttacgatt tcgatcgtgt tgactctggc aaaatccggt
     6961 ttgccaggag agggccagga catagcgatc aaaatcgcca tactgttttg cctgcaacgc
     7021 tacggcgttg gcagaagaga agggaagcag agaaaccgcg agcaacgcgg cgttacgcca
     7081 gaatgctttc ataatgggtg tggaactcat acatacactg aatactatct attaaatcat
     7141 aaaaaagccc gccaggtggg cttacctggc gggcgtgatg atttattcag cgtttggcga
     7201 acgtattagt tccacatggc gagaatcggc cagccaacca acagcaacat agagatgtaa
     7261 atcaccccga agattgcgcc aagacgccag taatcttttg atttcacata gccacagccg
     7321 taaataatca ccccaggacc ggttgcatac ggcgtcagac agcccatgat accgatagac
     7381 agcaccagca ggatacacag ttgttccatt ggtacgcccg gaataccttt accgacggcc
     7441 agaataaccg acagcatggt tgcggtgtgc gcagacaggc tggcaaacag gtagtgtgca
     7501 aagtagaaca ccagaaccag tacaatcacc gttgcgtttg gtgagaatcc ttcctggtgc
     7561 gtactcatgg taccggcgaa ccagtcaata aagccagaac gagtcaggcc gttagccatc
     7621 acaaccagag ttgccaggtt gaccagcgtg ttccatgcgc tgttatagcg ggtaatatct
     7681 ttccaaggca caacgtgcag ggccagcatt agcgaaactg ccagcagacc aactgcagta
     7741 gcattaatga cttcactgcc aaaaacccac aaacctaagc tgagcaatac aaggccaatc
     7801 agtgtccact ctctgcgtgt cagcgcaccc atggttttca gttcatcacc cgcccaggtt
     7861 gccacttctt cactgtgtgt gatttccggt ttgtacagca cgtaggaaag ccacggcgca
     7921 atgataagca agataacccc aaccggcagg aagcagagga accactgcaa ccagctaatc
     7981 tggataccgg caattttgct gacgaactcc agacccagca cgtttggtgc cgcaccggtg
     8041 acaaacatgg acgaactcag actggtacta atgaccatca tccacatcaa atagccgcca
     8101 atacgacgcg cggacggatc gttggggaat gatttaaaca gcggcggcag gtttttaatg
     8161 accggaaaaa ccgtaccccc ggtacgcgcg gtgttggacg gtgtaaacgg tgccagcaga
     8221 atgtcgataa tgacaatcgc ataacccaac gtcagcgtgc gtttgcccat gaatttcacc
     8281 aggaaaaggg caatgcgacg acctaacccg gaaacttcat accctaatgc aaaaataaat
     8341 gcgccaaata ccagccatac cgtggtgctg gaaaaaccag ccagacccca tttcagcgcc
     8401 tgtttttgcg cattaaacgc tgggtcagct aattctttgg catcaaagag caggtaatta
     8461 ctgccaataa cgcaaatagt aaccgcaata aaactgatcg ctgttgccgg aattggctcg
     8521 aggatcatgc cgacaatcat tgccacaaac acagcgaagt aatgccatgc ctgtggtggc
     8581 ataccgtcgg ggacagggat aagaaacatg acacccatca ccaccagtgg ggccaatagt
     8641 ttccatatat tatcttttgc taaagacata cgggttctcc gaaaattaat atttccaaat
     8701 ttatcaagtg cttaaataat taaatctgtg ctaaaaacca ggtaaggatc agcaggtcgg
     8761 cactgccgcc tggactgaga tttcgttcga tacactccct gtcgaactgc cggagataat
     8821 cgagatcggc gggggttcga atgccccctt tttgcaataa tgtttgcgcc tcgcgctgta
     8881 gccagcgcag gcccccctcg ccaccgcgcg atgcaaagtt ggtatcgccg ttgatcgcca
     8941 tcagtaggag caaggtatcg agcaatgcca gttcaggatc taacccctga tccagcagag
     9001 tgaggtaatg cggcaaggcg tgattgatca ccagtggata acccgcttcg gcttcaccgc
     9061 gtgcgccggt aaggccaagc tgctggtaca accgttgacc tgccgtcagt tgtgaattat
     9121 tggtacgcag ttctcgatcg gtcaggccac ggcagaaact tgccgccgta gaacaaacgg
     9181 ttgttggcgt taccggttgg ttgagttgaa gcaaacggcc aattgccgca catagtagcc
     9241 ctaaagaaaa aatgctgcct ttatgcgtgt ttacgcccgc agtggcgcgg aacatatcac
     9301 cttcgcaagc cataccaatt gggcgtaatc cgtggagtac cgcttctggt gctatttccg
     9361 cactacaggc accaaattca atgaaacggg gtagccagcc ctgaatcgcc agcgcgctgc
     9421 ggtggaaatc ttccagcgcc atatctttat gtgcaccgca gttaatccga tccacgaggc
     9481 ctggtttcgg tgacagattg acttcagtca gcatggcgcg ccagcccagc agggcgtact
     9541 catcgatcaa tgacgtcgca agctttgtgg ttttagttga cgttgcaggc atcgacatcg
     9601 ttcagcagtg cctccatgcg gttgagtaaa tcggtcagtt gatgggtttt tccacgcgcg
     9661 cagacggctg cgctttgctc gcacaacagg cagcggcgag gcggcagtga atagtcgcgg
     9721 cgggagagaa tttcgccttc gggcgtcagg acatcgatat cccataaccg cccgagagga
     9781 tgactatgtt caagctcaat ggtggcgagc ttgaggtcgc gagccggggc ggcaatgctc
     9841 aacatgccct ccggcccgct ggcggaaacc agtgcagcct gctcctgaat ttgccagccc
     9901 tgttttgcgg ctaaggcacg caaggctgtc acgccatgat taaaaattcg gcgtgtgacc
     9961 tcgctgtctt taatcggccc aggcgcaacc acggtaaagg agaccagtgg aacaggatgg
    10021 cgcttgagcc agacgtgttg ccgtgcttgc ctttcatccc ggctgacgag cagctcggga
    10081 attgacaccg catggtggct ggcgagttca ggaagcaggt gcatggctta ttccttcacc
    10141 tgatgcacaa catcgatcac cgagccatcg cggtaacgca caacggcaac gacgcggtcg
    10201 gtgaattcaa tcggctgtgg ttcaccggtc agcagacgcg cgcgttcgcg cagccactca
    10261 atggaaacca ctttaatgcc cgcttcctgc agacgttctg ccagttccgg acgtgccggg
    10321 ttaactgcga taccgtggtc tgtgaccaga atatcgacac tggagcctgg ggtgatgcag
    10381 gtcagcacgt tatccaccag agtcggaata cgaccgcgta ccagcggcgc gacgatgatg
    10441 gaaagcgcag aggcaatcgc ggtatcgcag tgaccaccgg aagcaccacg cagtacgccg
    10501 tcagagccgg tcagcacgtt aacgttgaac tgggtgtcaa tttccagcgc gctcagtacc
    10561 accacgtcga gacgatcaac cgatgcgcct ttcgaacccc agttagcgta ctggttggcg
    10621 ctgatttcga tgtgattggg gttacgggcc agcgattgcg cagcatggct gtcaaagctc
    10681 tgcacatcca gcagtttgcg gatcagacct ttttcgtgca gatcaaccat cgtcgcggta
    10741 ataccgccga gggcgaagtc ggcgcgaata tcgcggctac gcattttgtc ttccaggaaa
    10801 cgggttaccg ccagcgatgc gccgccggtg ccggtttgca tggagaaacc ttctttgaag
    10861 tagccagagt tgacaatcac atccgcagcg ctacgggcaa taagcagttc gcgcgggtta
    10921 gtggtcatac gggtcgcgcc agcgccgatt tttgcagcat cgccaacgcg gtcaactttg
    10981 acgatcaaat caacctgatc ttgctcaatg cttgccggat tatgcggata aggcagcagt
    11041 tcttcggtaa gcatcacaac ctgttttgcg ttgtcggcat caaccattgc atagccgagg
    11101 gagccgcagc aggctttacc ggtgtaaccg ttggcattac cgaattcatc acaggacggg
    11161 acgccgagga aagccacgtc gatattcagt tcgccgctct gtaccagatg cacacgtccg
    11221 ccgtgagagt ggatttgcac cggttccgca agcagaccac gggaaatttc ttctgccagt
    11281 ggaccgcgca ggccggaggt ataaatgcgg gtaaccacgc cctggcgaat gtgttctacc
    11341 agtggcgcat ggcaatcact cagggagctg gacgccaggg tcaggttttt aaagcccatc
    11401 ttagcgatga cgtccatcac catattgacg gtcaggtcac cgccacggaa agcgtgatgg
    11461 aaggaaaccg tcatgccgtc ctgtaaacca gagcgacgaa tcgcttcttc ctggttggcg
    11521 cacagtttgc gatcgcgcgc tttttcagcc tggtaggttt gctttggtga gttctggaaa
    11581 gcggcaagat cgcattcagc gcgacgattc caggccgcta cccgttcttg tcgttgagat
    11641 tgttcaattt tctgcgtcat tttgattgcc ttattcttcg cggatgccgg aaagttctgc
    11701 acgggagagc accagacggg cacgatcgat aaccggaccg tccaccatct tgccgttcag
    11761 ggaaaccacg ccgaggcctt cgcgagcggc ggcttcagcg gcttctacga cgcggcgggc
    11821 gtgatccact tctttctggg tcggtgcgta gaggttgtgc aacagatcaa tctgacgcgg
    11881 gttgatcagc gatttgccgt caaagcccag ctgtttgatg tgggcggctt cttgcaggaa
    11941 tccggcttcg ttgttagcgt cggaatagac ggtatcgaac gcctgaatac ccgcagagcg
    12001 cgcggcctgc aaaatggaac agcgtgcgaa cagcagttca gttccttccg gggagcgttc
    12061 tgtacgcagg ttgcgcacat agtcttctgc accgagggcg ataccgatca aacgctcgga
    12121 agcgtgagcg atttccactg cgcgggtaat ccccagcgga gattcaatcg ccgccagcag
    12181 gccggtgctg ccgggttcac gaccacaggc tttttcgata cgcaggatct ctttttcgat
    12241 atccagaaca tcctgagcgg tatcggtttt cggcagacgc acaacgtccg caccaccgcg
    12301 aacgacggct tccaggtcgt tgacacccca ttcggaatcc agcgcgttga cacgcacaat
    12361 ggtttcaata tcgcgataca gcggatgttg cagcgcgtgg taaaccatgc ggcgggcggt
    12421 gtctttttca cgcaatgcta cggagtcttc gaggtcaaac atcagggcat cagccgggta
    12481 gatgaaggag ttgctgacca tcgcggcatt ggcaccaggc acaaacaaca tgctgcggcg
    12541 ggtgcgagtt ttacgttgtt gcagcgaagc ggaaatcatt ggcaatcctc ccatggcaga
    12601 gccgggatac cgctggcgcg tgccagcagg gcttccagtc gtgcacgtaa aatgcagtcc
    12661 agtgcgcctt tgtcatcgac attcagctgt acgccgcgca cgttgtagcg ggcgagaacg
    12721 tccagaatgg tggtgcgaat tgcatcgcca aactgtttct caacgctgct attgatttgc
    12781 aggtcgatat cctgcgtatc gagtggggcg atgcgtatca tcacatcccc agactcaagg
    12841 gtgcctgcaa cggcgggctg gtttattttc atttttcacc tgtttctcat gcgggggtct
    12901 tttgacgagc tgccgcgtcc tggcgggagt gctcaagcag gttctgcaaa taatgcagcg
    12961 tgactgcagg gaccagcggc gcgatagccg tgagatcgtt tttcgccagc agttgacgta
    13021 cccgggaagc ggatatcggc atctcctggt aacgcagccg ctcaatttca accagttcga
    13081 tgggcggtgc ggagatagtc ggcgtttcca gccagtagcg catatcctgg ttgtactggg
    13141 cggtaacgcg acaaaagggt tcagtaccga caaagcggtg agttacaccc agcgcgggag
    13201 cgaggtactg acggaaaatc ttcagatcaa tttcggtgta acaatggtta atgacgctct
    13261 gttctttaat gaagtagcaa gggaacgtag cgcgggagat gatgtattcg gagccgcgat
    13321 gcacagtcag gcgtggaata tcggcggtgc cttttaacac cagatccaac cggtcttcat
    13381 aggggaagcg tgaagaatct tctttgacta aaaacagatg caaccagtcg cactgtgccg
    13441 cagcttgttg aatcagataa cggtgaccat tcgtgaaggg attggcgttc atcacaatgc
    13501 agccaatctt gttccctgga tgacgaaatt ttttcagcga ttcggcatag cgtttcagcc
    13561 gcgtggcgct gttttccatc agcaccatca cgccgggtac gctggtcagc gtggaaaaac
    13621 cgcactggcg gaacagcgcc tcgtattcgg ttttggtata aataaacaga tgcgtgctgt
    13681 gccgctcata ggcgaggttt atcaattcag tggctaatgt cagcgccagt ccttcaccgc
    13741 gaacggattc actgatagca acgcatttaa taatatttcc ggcaattcca ccgcacgtga
    13801 taagcttttc atcgcgggtt acggtaataa atacttcgac tgtggtgtca acg
//
LOCUS       DAZRUF010000101        13807 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZRUF010000101 DAZRUF010000000
VERSION     DAZRUF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.101"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..1727
                     /gene="adeD"
                     /locus_tag="ACHXOL_002360"
     CDS             <1..1727
                     /gene="adeD"
                     /locus_tag="ACHXOL_002360"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR6809323.1"
                     /translation="AEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRY
                     IAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVI
                     CDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAW
                     RDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIE
                     NCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWE
                     IAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSD
                     ARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGK
                     RYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGLNE
                     GALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLM
                     STDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDVEKFA
                     FTTLEVTE"
     gene            complement(1772..3163)
                     /gene="uhpT"
                     /locus_tag="ACHXOL_002361"
     CDS             complement(1772..3163)
                     /gene="uhpT"
                     /locus_tag="ACHXOL_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR6809324.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3301..4620)
                     /gene="uhpC"
                     /locus_tag="ACHXOL_002362"
     CDS             complement(3301..4620)
                     /gene="uhpC"
                     /locus_tag="ACHXOL_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR6809325.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4630..6132)
                     /gene="uhpB"
                     /locus_tag="ACHXOL_002363"
     CDS             complement(4630..6132)
                     /gene="uhpB"
                     /locus_tag="ACHXOL_002363"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR6809326.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6132..6722)
                     /gene="uhpA"
                     /locus_tag="ACHXOL_002364"
     CDS             complement(6132..6722)
                     /gene="uhpA"
                     /locus_tag="ACHXOL_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR6809327.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6797..7087)
                     /gene="ilvN"
                     /locus_tag="ACHXOL_002365"
     CDS             complement(6797..7087)
                     /gene="ilvN"
                     /locus_tag="ACHXOL_002365"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR6809328.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7091..8779)
                     /gene="ilvB"
                     /locus_tag="ACHXOL_002366"
     CDS             complement(7091..8779)
                     /gene="ilvB"
                     /locus_tag="ACHXOL_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR6809329.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVEE"
     gene            complement(8885..8983)
                     /gene="ivbL"
                     /locus_tag="ACHXOL_002367"
     CDS             complement(8885..8983)
                     /gene="ivbL"
                     /locus_tag="ACHXOL_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR6809330.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9548..9637
                     /gene="tisB"
                     /locus_tag="ACHXOL_002368"
     CDS             9548..9637
                     /gene="tisB"
                     /locus_tag="ACHXOL_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR6809331.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9761..9835)
                     /gene="ysdE"
                     /locus_tag="ACHXOL_002369"
     CDS             complement(9761..9835)
                     /gene="ysdE"
                     /locus_tag="ACHXOL_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR6809332.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9917..11101
                     /gene="emrD"
                     /locus_tag="ACHXOL_002370"
     CDS             9917..11101
                     /gene="emrD"
                     /locus_tag="ACHXOL_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR6809333.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11109..11606)
                     /locus_tag="ACHXOL_002371"
     CDS             complement(11109..11606)
                     /locus_tag="ACHXOL_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR6809334.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQEMPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11603..11965)
                     /locus_tag="ACHXOL_002372"
     CDS             complement(11603..11965)
                     /locus_tag="ACHXOL_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR6809335.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11955..12302)
                     /locus_tag="ACHXOL_002373"
     CDS             complement(11955..12302)
                     /locus_tag="ACHXOL_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR6809336.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12411..12860
                     /gene="yidI"
                     /locus_tag="ACHXOL_002374"
     CDS             12411..12860
                     /gene="yidI"
                     /locus_tag="ACHXOL_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR6809337.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12907..13065)
                     /locus_tag="ACHXOL_002375"
                     /pseudo
     CDS             complement(12907..13065)
                     /locus_tag="ACHXOL_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13109..>13807)
                     /locus_tag="ACHXOL_002376"
     CDS             complement(13109..>13807)
                     /locus_tag="ACHXOL_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR6809338.1"
                     /translation="TPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARL
                     QRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVS
                     NMSKSAAGTVSLPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYT
                     SQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
BASE COUNT         3229 a         3847 c         3620 g         3111 t
ORIGIN      
        1 gggctgaata ccaggaattg ttagccgttt cccgtggcga cgctgttgcc gattatatta
       61 ttgataatgt ctctattctc gacctgatca atggcggaga aatttccggc ccaattgtga
      121 ttaaaggacg ttacattgcc ggtgttggcg cagaatacgc tgatgctccg gctttgcagc
      181 ggattgatgc tcgcggcgca acggcggtgc cagggtttat tgatgctcac ctgcatattg
      241 aatccagcat gatgacgccg gtcacttttg aaaccgctac cctgccgcgc ggcctgacga
      301 ccgttatttg cgaccctcat gaaatcgtca acgtgatggg agaagccgga ttcgcctggt
      361 ttgcccgctg tgccgaacag gcaaggcaaa accagtactt acaggtcagc tcttgcgtac
      421 ccgccctgga aggctgcgat gttaacggtg ccagttttac ccttgaacag atgctcgcct
      481 ggcgggacca tccgcaggtt accggccttg cagaaatgat ggactaccct ggcgtaatta
      541 gcgggcagaa tgcgctgctc gataaactgg atgcatttcg ccacctgacg ctggacggtc
      601 actgcccggg tttgggtggt aaagaactta acgcctatat tactgcgggt attgaaaact
      661 gccacgaaag ttatcagctg gaagaaggac gccggaaatt acaactcggc atgtcgttga
      721 tgatccgcga agggtccgct gcccgcaatc tcaacgcgct ggcaccgttg atcaacgaat
      781 ttaacagccc gcaatgcatg ctctgtaccg atgaccgtaa cccgtgggag atcgcccatg
      841 aaggacacat cgatgcctta attcgccgcc tgatcgaaca acacaatgtg ccgctgcatg
      901 tggcatatcg cgtcgccagc tggtcgacgg cgcgccactt tggtctgaat cacctcggct
      961 tactggcacc cggcaagcag gccgatatcg tcctgttgag cgatgcgcgt aaggtcacgg
     1021 tgcagcaggt actggtgaaa ggcgagccga ttgatgcgca aaccttacag gcggaagagt
     1081 cggcgagact ggcacaatcc gctccgccat atggcaacac cattgcccgc cagccagttt
     1141 ccgccagcga ctttgccctg caatttacgc ccggaaaacg ctatcgggtc attgacgtca
     1201 tccataacga attgattacg cactcccact ccagcgtcta cagcgaaaat ggttttgatc
     1261 gcgatgatgt gtgctttatt gccgtacttg agcgttacgg gcaacggctg gctccggctt
     1321 gtggtttgct tggcggcttt ggactgaatg aaggtgcgct ggctgcgacg gtcagccatg
     1381 acagccataa tattgtggtg atcggtcgca gtgccgaaga gatggcgctg gcggtcaatc
     1441 aggtgattca ggatggcggc gggctgtgcg tggtacgtaa cggccaggtc caaagtcatc
     1501 tgccgttgcc cattgccggc ctgatgagca ccgacacggc gcagtcgctg gcggaacaaa
     1561 ttgacgcctt gaaagccgcc gcccgtgaat gcggtccgtt acccgatgag ccgtttattc
     1621 agatggcgtt tctttctctg ccagtgatcc ccgcgctaaa actaaccagt caggggctat
     1681 ttgatgtcga gaagtttgcc ttcactacgc tggaagtcac ggaataataa aaaagcccgg
     1741 cgtcatgccg ggcaaaagtc accagttacg tttatgccac tgtcaactgc tgaatttttt
     1801 tctcgcggcg gattttgcgt tcttccatca ccgccactat cgccatcagg cagatacaac
     1861 caatcgcggc gatatcaagt gcggcgaagg tgcctgccca gccggtaagg ccgaataccg
     1921 gcgtcccatc ggcaatcatt cccagaccta acttggcaaa gctgtcacca atcaggtaag
     1981 caaaggtgcc tttaatacca tcggcagcgc caatcgcttt tttaggtaca aagccaacag
     2041 cagccacacc aatcaacaat tgcgggccaa agaccaggaa acccaacgca aagagagaag
     2101 ccagatagat atattcgtta ctggcatgtt gatacacacc gagcgtggcg ataatcagcg
     2161 ccagcgcgat gcaggccacc aggccacggc gaccgttcgc taggtcagag agccagcccc
     2221 acagcagcgt accgaccagc gcaccagctt caaacagcgt aaagccctga atcgccaccg
     2281 ctttagagag tttcagttcc tggaacgcgt atacggttga ccactggtcg ataccaatac
     2341 gtaccacata gaggaaaatg ttggcgaagc acagcagcca gatcactttg tttttcagca
     2401 catactcaac aaagatctgc cacttggtca tatcggtaga ttctgtctct ttgtcctctt
     2461 cgctgatctc ctcgccgaac agttcttcag ctttgccgag gccataagat tccggggagt
     2521 cgctgccgta acgcaggccg ataaaaccga caatcagcgc gataatcgac gggaagataa
     2581 acatgccgat gacatggcca tcgaacaggt aatttgcccc gaacagcgcc acgcctgctg
     2641 cgcctgcacc gccaaggttg tgagaaatat tccagaaacc gaggaatgtc ccgcgtttac
     2701 gacgcggcgt ccatttggtg atggtggagt aactgcacga accgccggta ctctggaaaa
     2761 agccgcttaa ggcgtagaag gcaatcatca ggaacaggct aaccgagccg ctgcccatac
     2821 tggcactgaa gcccagcata caaatggcag acaggatcag catgaacggc aggaattgtt
     2881 tggtgttttt gccgtcggcg tagtaggaaa ccagcgtttt acccacgcca taagtgatgg
     2941 agaaacccag gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc gagatcatgt
     3001 cgttctgcgc gatgttaaag ttcttgcgaa tcaggtacat cgtcaggtag ccgataaaga
     3061 ccaccaggta ggattgcatg aacggtttga accacatttt gcgccgcact tcgagcggaa
     3121 ggtccagggt cggcttgcga acctggttta agaaagccag catgggttac tcctgaaatg
     3181 aatacctgcg tgaggcatgc attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc
     3241 caggcaaaac ctggaaaatt tcttagtttt gccggatatg aagtgaaaag gtgagatgca
     3301 tcacgcttcg cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg cggaaatccc
     3361 ggcggcgata gagataacca caaaaaatcc gctccagtgc caggtatcga gtactttcgc
     3421 cagcggccaa ccagcaagcg acgcccccag ataagcaaac aagccgacaa accccgtcgc
     3481 cgcccctgcc gcctctttgt gggaacactc tgccgccgcc ataccgatta acatctgtgg
     3541 gccaaagaca aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa
     3601 tggcatcagc cacagggagc cgactgaaag caaaattccg gcggcgaaaa tcaaattcat
     3661 cggccctcgg ttgccgttaa acaatttgtc cgagccccaa cccgctacca gcgcaccgat
     3721 aaatccaccc agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat cgacgcccag
     3781 cgtctcggac atatacaaat tgccccagtc gttgatcgcc gcccggacca catagaccag
     3841 cacatagcaa aacgaaagca gccagatata cggattcagc aacacatatt tggtgaggat
     3901 ctctttacgc gtcagccctg ccccttcttg ttgttgagct atctccagcg cgtcgtgtcg
     3961 ccattcaccg accgccggta aacctaacgc ctgcgggcga tcgcgcagcc gccagcagag
     4021 aaaaattccc acgacgatcg ccatacaacc agcaatcatc atcccggcac gccagccgta
     4081 atgtagtgca gccgctgcca tcacaatggg aatgagtgcg ccgccgacgt tatgcgccgt
     4141 gttccataat gcccaccaac caccgcgctc ggtacgtgaa taccaggccg ttaacagacg
     4201 cgcacacacc ggtgaacccc agccctggaa aaaggcgttc agcacccaga gtacggcaaa
     4261 cgcccatagc gacgtcgaga agccaaacag aatgttgata atgcccgtgg cgataagccc
     4321 tatccccata aaataacggg catttgagcg atcgctgaca atgccggaga caaacttcga
     4381 cacgccatag gtaatgtaaa acagggtcgc taacaggccg atatcgctac ggctgagcac
     4441 gccgttagca aggatttctg gtacggcggc gttaaaactt ttccgcgtga agtaaaacag
     4501 cgcgtaaccc agccagatgg tcagcagaat atgccgacgc cagtagcgat agcgggcatc
     4561 aatttcatat ttatcagcca ttaatggcgc atcggcaggc gctttcagaa acggcaacat
     4621 catcaaacct tagacatagc gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca
     4681 ggaaatgtgt aatgtgccac ccagcgccgt tacgcgctcg cgcattccgg tgaggccaaa
     4741 accttgttgc ccggaacccg gcggcaaacc gctgccatcg tcttcaataa ccagcatcaa
     4801 ccgttcatcc tgctgccagc cttgcagggt gaccgcgctg gcatcagcat gtttcacaat
     4861 gttgttcagc ccttcctggc agacacgaaa cagcgtcacg cgctggtttt cgcttaacgc
     4921 tgattcatcg attcgccatt cgagatggct gacaataccg cgcccttcca gctccatttc
     4981 ccgcatcagt gagcggatgg cctgctccag ggtgagatca tccaactggc gcggacgtaa
     5041 ccgacccaac aaacggcgca ccgcgtcgta aacgcccagc gatagttgtt cgatgagttg
     5101 cccgctctgc ttcacgctgg cgttatctgc cgccagccgc tgaacaatgc ccgcctgagt
     5161 acgaatagca gtgatggtct ggccgatatc atcatgcagc tcacgcgcca catcacggcg
     5221 cacgctctct tcggtttcca gcaaccgttc agccagatgc tgattgcgcg ccagttcctt
     5281 ttgcagcgac tggttaagtt cacgcaaccg ctggatgcca gcgccaagca acaaccctgt
     5341 cagactttgc accagcagcg agagcaataa atccaccgga tgatcgcgcc aggtttgact
     5401 ggcgatcagc gcgatggcgt tcatcaacgt cgcaatcagc gccccttgcc aaccatagtg
     5461 ccaggccagc gcgataatcg gcagcaccag acagaatggc gtaaagcgcg acagttcgtc
     5521 cggcaatccc aactggagcc agagactgat aacaaacagc agcaagtacc agaccagatg
     5581 tcgcccgcgc cagttgattg gctgagaaac cagtgacggg ccgagcggca gccaggtgtt
     5641 attggcgaga tagtgccaga acaccagaca tatcggggcc agcgtcaggc cgccagttaa
     5701 agtcagcaac agcgcattcc acgactcttt gccgtgccaa agccagggca gcgactgcaa
     5761 caacgccgcc gccgttaacg ccgccccctg taacagcaag gtgcgccagt cacgctgatg
     5821 gcgatagcgc gagatcagcg ctacgggcag taacgtcagt aaactaccga tcatcaataa
     5881 cggaaaatgg gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc actccgcacc
     5941 cagcaatacg ggccagtatc cgcgcgggca ttgcagcatt agccccagac gcagaccaaa
     6001 cggaaataac agcaccgcca tatcagggcg ctcaaccaga tgcaggctga tactccacag
     6061 gcaaaaccat gcggcagaga agataaaaaa gcaggcaata acggtaatta agcgggagaa
     6121 caacgtcttc atcaccagcc atcaaacatg cggcgcgcca gctctacgtc gttactgacg
     6181 cccagttttt ccatcagatt ggcgcgatgg acgtgtaccg ttttcggtga caagcccagt
     6241 tcggcggcaa tctctttcac cgccattcct tgcgccagtt tttccgccac ctggcgttca
     6301 cgtttggtta gcgggtcctg acgaccggat gccagtttaa tggcaatatc cggcgtcaga
     6361 taacagccac ccgtggcaac cgtatgcacc gcagcaatga gttcatccgg gctacagcgt
     6421 ttggaaagaa agccgcgtgc ccccgcgtta agcgcctgct caaccagcgc aggactgtcg
     6481 tgaacggaga gcataatcgt cgccatacct ttcggcagct ggcttagcag ctccagaccg
     6541 gagatatcgg gcatggagat atcgcaaata cacacctgca caccgcgccc cggcagcccc
     6601 gccagcgcct cgcgccccga accaaactcg gcaactacct gcaaatcagg ttccagcccc
     6661 agcagctgcg caaagccgga gcggacgatg aggtgatcgt ctataagggc aacggtgatc
     6721 atggtcttgt cctggcgggt aaaaaaaaac gcgcttacct taacgataag cgcgatgttg
     6781 ttcaagcctt gagcggttac tgaaaaaaca ccgcgatctt gttaaacatc gtcggatcgg
     6841 actgattacg ctgcactttc acgacatctt ccagcttatc gatttggctt atcatctgct
     6901 ccagacgctg gtcgtcattg accagtagcc agatatggct tttgtcgctg tcctgaatcg
     6961 gcagacaaag aatgccttca acgttaaaag cgcggcgggc aaaaaggcca caaacgtggg
     7021 tcattacgcc cggatggttg cgaacggtga gctccagaat tacgttgtca tgagttgtgt
     7081 tttgcatggc ttattcctcc accatttcag tattcgccgc acctggcggc accatcgggt
     7141 aaactttttc ttcggcatca atgcgcacat ggatcagcgc cgggctaggg cgattgatga
     7201 tttcctgcaa tgcagcctgc ggatcggctt cgttattcaa atcacaggtt tcgaggccga
     7261 atccggcggc aatctgcata aagttgattt tgcccggata ggtggcggca aaaacgcctt
     7321 gctcgtagaa cagactctgt tgctgatgca ccagccccag cgcttcgttg ttcatcagaa
     7381 tgattttgac atccagctga ttttcactgg cggtcgccat ctcctgaata ttcatcatca
     7441 ggctgccgtc gccggagaaa cacaacactt tgcgatccgg gttcgccagc gccgcgccaa
     7501 tcgccgcagg caggccaaaa cccatcgtgc ccagcccacc ggaggtcagc cactggcgtg
     7561 ggcgattgag cggataagct tgcgcggtcc acatctgatg ctgacccaca tcggtggtga
     7621 taatcgcatt gtcatcgaca caggcggcaa cggcgttgat caggccgtaa tggcttaatg
     7681 gatcgcacgc tttcgggatt ggacacggga attcgcgctg caaatccgct accaactggt
     7741 gccactctgc acgcggttgc gcttccacct gcgggatcaa ttgcgccagc acgtcatcaa
     7801 catccgcctg aatcgccaca tgcggctgct tgattttacc cagctctgcg cgatcgatat
     7861 cgacatgaat aattttggca ttcggacaga actgctcggt tttgccaatc gcccggtcat
     7921 caaaacgcgc accgagcaca atcaacaaat ccgcctcctg caaaatatag ttggtactgc
     7981 gcacgccgtg catccccagc atacccagcg acaacggatg cgcttttggc agcatgccca
     8041 gcgccattaa agtcatggtg gtaggcagtt gcgctttctc cgccagttca cgcacccgcg
     8101 caggcgcatt gatcacacca ccacccagat aaagcaccgg gcgtttggca gcgttaatca
     8161 ttgtagctac gtcacgaatg ctttcttcgc taaaggcggg tgcagcggct ttttctgcca
     8221 ccgcgggctg agcttcaatc tcaaaaaccg ccgtttgtac atccttagga atgtctatcc
     8281 acaccgggcc agggcggcct gattgcgcaa tgcggaaggc atcgctcatg acctgcggga
     8341 gttcttcgat atgtctgacc agatagttgt gtttggtgat ggggatagag atgccgtagg
     8401 tgtcgacttc ctggaaggcg tcggtgccga tcatcgaggc gggaacctga ccagtgatgc
     8461 aaatcagcgg gatggagtcc agccgcgcat cggcaatggc ggtcaccagg ttagtcgcac
     8521 ccggtccgct acaggccata cagaccgccg gtttaccgtc ggtgcgcgcc attccctgag
     8581 cgataaagcc cgcgccctgt tcatgacggg ccagaatatg gcggatttgc gtgctttggc
     8641 ttaaggcatc gtaaacaggc aggatagaac cgcccggaat gcctgtcaca atcttaatgc
     8701 cctgctgttc caggaaatga acgataaatt ctgcgccggt aaagcgctta cgcgtcaatg
     8761 ttgtgcccga acttgccatg ctccagtcct tttcttctgg gccgactttc cgggaggtgc
     8821 ttaaacgaaa aaccccgccc ggtttgcgcc gacggggttt tggaatcgtg tgttgttcca
     8881 gtccctacgg cgcattgccg acgaccacca ccacacgcac gacgaccact gcggcggatg
     8941 gcgcagttgg tagtagtttt gcgttgaaca tggaagtagt cattggggac cttgttggtt
     9001 ttgtgtttaa caatatttat acaagcacag ctttacaggg gagacaatgg aaaatttttc
     9061 agcaagggaa aattgagggg ttgatcacgt tttgtactga attgcagata acaaaaaacc
     9121 ccgccggagc gaggtttcgt cagtcgcccg cggctggtaa ccgcaaagca cactgtatta
     9181 tgtcaacaca gaaagtatac gtgttccgcg cagaacgcgc aatttcggca cgaattttga
     9241 cgtatttagt gcatagttga gtatcgatca cagtttgcgt tttgtccaaa tattactgtt
     9301 tatttataca gtaaacttct ataatatcac tgtacgcaat gtgttatgcg ggggccgcat
     9361 cgttacccgg cgcactaagt cctggctgaa acgggtggtg ccgtcagcgc cttaaccccg
     9421 cgtgagcaca ctgtgttatg tcaacaagca caaagtttct ccttgagata ccgcgtgcac
     9481 aacagctggc aacaggcagc ggaaaggtac gtcagctggc agtgctcctg aaccacagga
     9541 gacgcgtatg agcctggtgg atatcgccat tcttatcctc aaactcattg ttgcagcact
     9601 gcaactgctt gatgctgttc tgaaatacct gaagtaattc agattcaagt cgcaccaaag
     9661 gggagcgggt aaccgctccc cttttatatt tagcgtgcgg gttggtgtcg gatgcgatgc
     9721 tgacgtatct tatccgccct accatctctc ccggcaacat ttattgccgc ttttgtttac
     9781 atattctgcc gctaaacaac tccccattcc tggcgtatat ctggctaaca ttcatcaatg
     9841 tgatagattc ctctcccgca tttatgggaa tgcgtagtga cttactctaa ttatttttat
     9901 aaaagcatcc gtgataatga aaaggcatag aaacgtcaat ttgttattga tgttggtatt
     9961 actcgtggcc gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc
    10021 gcgcgatctc aacgtccgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac
    10081 ttacggtgtc tcacagctgt tttatggccc gatttccgac cgtgtgggtc gccgaccggt
    10141 gatcctcgtc ggaatgtcca tttttatgct ggcaacgctg gtcgcggtca cgacctccag
    10201 tttgacggta ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt
    10261 aatggcgcgt actttgccgc gagatttata tgaacggaca cagttgcgcc atgctaacag
    10321 cctgttaaac atggggattc tcgtcagtcc gttgctcgca ccgctaatcg gcggtctgct
    10381 ggatacgatg tggaactggc gcgcctgtta tctctttttg ttggttcttt gtgctggtgt
    10441 gaccttcagt atggcccgct ggatgccgga aacgcgtccg gtcgatgcac cgcgcacgcg
    10501 cctgcttacc agttataaaa cgcttttcgg taacagcggt tttaactgtt atttgctgat
    10561 gctgattggc ggtctggccg ggattgccgc ctttgaagcc tgctccggcg tgctgatggg
    10621 cgcggtgtta gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc
    10681 agcgtttttt ggcgcatggt ttgccggacg tcccaataaa cgcttctcca cgttaatgtg
    10741 gcagtcggtt atctgctgcc tgctggctgg cttgctgatg tggatccccg actggtttgg
    10801 cgtgatgaat gtctggacgc tgctcgttcc cgccgcgctg ttctttttcg gtgccgggat
    10861 gctgtttcct ctggcgacca gcggcgcgat ggagccgttc cccttcctgg cgggcacggc
    10921 tggcgcgctg gtcggcggtc tgcaaaacat tggttccggc gtgctggcgt cgctctctgc
    10981 gatgttgccg caaaccggtc agggcagcct ggggttgttg atgaccttaa tgggattgtt
    11041 gatcgtgctg tgctggctac cgctggcgac gcggatgtcg catcaggggc agcccgttta
    11101 agcgcacgtc accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga
    11161 tcacgcatca cccgcatatg cggtgcggag taagaataaa acgcctgata gcccgcacaa
    11221 agcacgctgt ctggcatctc ctgccgagcg tgcgggcaca ggcgatgcca gtcgcacgcc
    11281 tggcagtcac agcgcggttg cgggttttcg ctgccacacc atttttgtaa ggtggcgtca
    11341 aaaagctcaa tcgagatacg actgatatct gcccgcaccc aacactcgaa cagggcaatt
    11401 aaaaatttcc cccacagctc tgctgtcacc gaagcggccg tcagatcgcc ccggtcatca
    11461 aattcaagat agggaatgag ctgtacggag cggatgcccc gctgtttgaa attgtgataa
    11521 aaatcggcag gttgcagagc atccttgtac tctacaacca gtttatgcct gtcctcttca
    11581 gcatcaatga cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta
    11641 tatgcgttac agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt
    11701 cacggactac gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac
    11761 gggtgtagtg ccagaggatc agcgccacga tggcgagaat gccaatcgaa atccagaata
    11821 acatgcccgc ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca
    11881 gcatggtacg aaaccacgcc agtgatgtgc gctccggctg cagtcctggg tcggcaatgc
    11941 gtctggcttt gcggctatcc ggcatacaac accagtccca taacgatcac cgccacgacc
    12001 atcaatatta agctaatgat taataagctg ttggtgtagg gcaggtcctc tttcagacgc
    12061 atcgcttttt cattgcgtaa ccagcgcagg tagccataca ttgctaaccc gccggaaaac
    12121 agacacaaca acaacgccag cagttcacga atgacgggcg tggcgaaatc tggcgcaagt
    12181 tgatcaagcc caacccccgc cgccagaaat cctagtgcgg tacggatcca cgccagaaaa
    12241 gtacgctcat tagccagcga gaagcggtaa tcgggcgctt ctccaaggcg ggaaattttc
    12301 attgttgctc ctttgcaatt tcactaactc tctaccttta gcggtttttt ttaagaattt
    12361 cgtccgcccg tttcctgata tgcttttgcc atccgctaca aggaggtcag atgggtatca
    12421 tagcgcaaaa taaaatcagt tcattgggca tgctgttcgg tgccatcgct ttaatgatgg
    12481 ggattatcca cttttcattc ggcccttttt ccgcaccacc gccaacgctt gaaagcatcg
    12541 tggcggacaa aacggctgaa attaaaagag gtcttctcgc cggaataaaa ggcgaaaaaa
    12601 ttaccaccgt ggagaaaaaa gaggacgtag atgtcgataa aatcctcgat caaagcggta
    12661 tcgcactggc cattgctgcg ctactttgcg catttgttgg cggtatgcgt aaggaaaatc
    12721 gctggggcat tcgcggcgcg ctggtttttg gcggcggaac gctggctttc cataccttgc
    12781 tttttggtat cggcatcgtg tgcagcattt tgctgatatt ccttatcttt tccttcctga
    12841 ccggcggttc gctggtttaa gcagcctgaa taatgcccgc cgggtgatca tcagcgggca
    12901 ttatcgtcag aacttctgtt ttttctcttc caccttcacc ccttgtgtcg gtaggccggt
    12961 gtcatagtca cgtaccaccg gcgaatagcc atcttgtggg cgtggacgaa atgcccccat
    13021 ccagcgcggc cgtgcatctt tacgccacgg acgcaggctc cattgttgac atcctccacg
    13081 ccctgaagga cgtggacccc tgctacgttc aggctgtcgc ctgaatcatt tcggtgggtt
    13141 cctgcttcaa cgagcggcct gactgcacca tctctccaca ggcaagaacg gcgtgccccg
    13201 ccgctaaaat gttacgagcg ccatttacat cggcgttcgc tgtatatcca catacctggc
    13261 atctgaattt actttgtgac aggcgatttt ctttcgctgt atgaccacag cacacgcaac
    13321 gctggcttgt gtacgctggc ggaacagcaa gcacctgacc accacgccag agctgcttgt
    13381 acgcaagctg gcggcgcatt tcataccagc cctgatccag tatcgaacgg tttaaacctg
    13441 attttgcccg gacattgcgc cccggcaggc tgaccgtacc cgctgctgac tttgacatgt
    13501 tgctgacctt caaatcctca atgactatca ttgcgtggtt tttgctgacg gtcgttgtga
    13561 ctttgtgaag gtagtccctg cggatatttg cgatacagga atgcagtcgc tgtattttgc
    13621 gtttctgctt ctgccagttg ttgctgaatt tgaccttgcg gcttaactgt cgctgaagtc
    13681 tcgccagcgt cttctggttt ttctgaaaac tgtttacagg ctcaaagact gtgccatctg
    13741 acagcgtggc gagtttagcc acgccagcat ccagcccgac cattgatgct gaagggtgag
    13801 ccggagt
//
LOCUS       DAZRUF010000102        13711 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZRUF010000102 DAZRUF010000000
VERSION     DAZRUF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.102"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..94)
                     /gene="yojO"
                     /locus_tag="ACHXOL_002377"
     CDS             complement(<1..94)
                     /gene="yojO"
                     /locus_tag="ACHXOL_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR6809339.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNG"
     gene            203..697
                     /gene="napF"
                     /locus_tag="ACHXOL_002378"
     CDS             203..697
                     /gene="napF"
                     /locus_tag="ACHXOL_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR6809340.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            687..950
                     /gene="napD"
                     /locus_tag="ACHXOL_002379"
     CDS             687..950
                     /gene="napD"
                     /locus_tag="ACHXOL_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR6809341.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            947..3433
                     /gene="napA"
                     /locus_tag="ACHXOL_002380"
     CDS             947..3433
                     /gene="napA"
                     /locus_tag="ACHXOL_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR6809342.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3440..4135
                     /gene="napG"
                     /locus_tag="ACHXOL_002381"
     CDS             3440..4135
                     /gene="napG"
                     /locus_tag="ACHXOL_002381"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR6809343.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4122..4985
                     /gene="napH"
                     /locus_tag="ACHXOL_002382"
     CDS             4122..4985
                     /gene="napH"
                     /locus_tag="ACHXOL_002382"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR6809344.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4982..5431
                     /gene="napB"
                     /locus_tag="ACHXOL_002383"
     CDS             4982..5431
                     /gene="napB"
                     /locus_tag="ACHXOL_002383"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR6809345.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5441..6043
                     /gene="napC"
                     /locus_tag="ACHXOL_002384"
     CDS             5441..6043
                     /gene="napC"
                     /locus_tag="ACHXOL_002384"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR6809346.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6056..6679
                     /gene="ccmA"
                     /locus_tag="ACHXOL_002385"
     CDS             6056..6679
                     /gene="ccmA"
                     /locus_tag="ACHXOL_002385"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR6809347.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6676..7338
                     /gene="ccmB"
                     /locus_tag="ACHXOL_002386"
     CDS             6676..7338
                     /gene="ccmB"
                     /locus_tag="ACHXOL_002386"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR6809348.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7380..8117
                     /gene="ccmC"
                     /locus_tag="ACHXOL_002387"
     CDS             7380..8117
                     /gene="ccmC"
                     /locus_tag="ACHXOL_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR6809349.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8114..8323
                     /gene="ccmD"
                     /locus_tag="ACHXOL_002388"
     CDS             8114..8323
                     /gene="ccmD"
                     /locus_tag="ACHXOL_002388"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR6809350.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8320..8799
                     /gene="ccmE"
                     /locus_tag="ACHXOL_002389"
     CDS             8320..8799
                     /gene="ccmE"
                     /locus_tag="ACHXOL_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR6809351.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8796..10739
                     /gene="ccmF"
                     /locus_tag="ACHXOL_002390"
     CDS             8796..10739
                     /gene="ccmF"
                     /locus_tag="ACHXOL_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR6809352.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10736..11293
                     /gene="dsbE"
                     /locus_tag="ACHXOL_002391"
     CDS             10736..11293
                     /gene="dsbE"
                     /locus_tag="ACHXOL_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR6809353.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11290..12342
                     /gene="ccmH"
                     /locus_tag="ACHXOL_002392"
     CDS             11290..12342
                     /gene="ccmH"
                     /locus_tag="ACHXOL_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR6809354.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12377..13024)
                     /gene="narP"
                     /locus_tag="ACHXOL_002393"
     CDS             complement(12377..13024)
                     /gene="narP"
                     /locus_tag="ACHXOL_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR6809355.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13344..>13711
                     /locus_tag="ACHXOL_002394"
     CDS             13344..>13711
                     /locus_tag="ACHXOL_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR6809356.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         2926 a         3458 c         4032 g         3295 t
ORIGIN      
        1 aaccatttga aatgtgagca aaagcccgtt tttgccacgc ttcccgccac tcttttgatc
       61 ctgctacagg ttttaccccg atcggggtat gcatctttga cacatccttt aatatcttag
      121 cggctataaa aatggcttat taattatgcg gtttatttgg tcgctctcaa ttttcagagc
      181 gcgttaatga tggaaggtca atgtgaagat tgatgcatcc cgtcggggca tactcactgg
      241 tcgctggcgc aaagccagta acggtatccg tccgccctgg tccggtgatg aatctcattt
      301 tctgacccat tgcacccgtt gcgacgcctg tattaatgct tgtgaaaaca acattctgca
      361 acgcggcgcg ggcggctatc cgagcgttaa tttcaaaaat aacgagtgca gcttctgtta
      421 cgcctgcgcg caggcttgcc ccgaatcgct attttctccg cgccacacca gggcctggga
      481 tttgcagttc accattgggg acgcgtgcct ggcgtatcag tcagttgaat gccgccgctg
      541 tcaggatagc tgtgagccaa tggcaattat ctttcgcccg acgctctccg ggatctacca
      601 gccgcaactt aatagccaac tttgcaacgg atgtggagca tgtgcagcca gctgtccggt
      661 atcagccata actgcggagt atctccatgc acactaactg gcaagtttgc agcctggtcg
      721 tgcaggccaa aagcgaacga atttcagaca tcagcaccca actgaacgcc tttcccggct
      781 gtgaagttgc tgtcagcgac gcgccgagcg gtcagttgat tgtggtggtg gaagcagaag
      841 acagcgaaac gctgatccaa accattgagt cagtacgcaa cgtagagggc gtgctggcgg
      901 tgtcgctggt ttatcaccag caggaagagc aaggtgagga aacaccatga aactcagtcg
      961 tcgtagcttt atgaaagcta acgccgttgc ggccgctgcg gcggctgccg gtctcagcgt
     1021 gccgggcgtt gcccgcgccg ttgttggtca gcaggaagcc attaaatggg ataaagcgcc
     1081 gtgccgtttc tgcggtactg gttgcggcgt tctggtcgga acgcagcagg ggcgtgtggt
     1141 ggcctgtcag ggcgacccgg acgcaccggt taaccgtggc ctgaactgca ttaagggcta
     1201 tttcctgccc aagatcatgt acggtaaaga ccgtttgacg cagccgctgc tgcgtatgaa
     1261 aaacggtaaa tatgacaaag aaggcgaatt taccccaatc acctgggatc aggccttcga
     1321 tgtgatggaa gagaaattca aaaccgccct gaaagaaaaa gggccggaat cgatcggtat
     1381 gttcggttct ggtcagtgga ctatctggga aggttatgcc gcgtccaagc tgtttaaagc
     1441 gggcttccgt tcgaacaaca tcgacccgaa cgcgcgtcac tgtatggcgt cggcagtagt
     1501 tggctttatg cgtacctttg gtatggatga gccgatgggc tgctatgacg acatcgagca
     1561 ggctgacgcg tttgtgctgt ggggcgcaaa catggcggag atgcacccga tcctctggtc
     1621 gcgcatcact aaccgtcgtc tctctaatca gaacgtcacc gtggcggtgc tttctaccta
     1681 ccagcatcgt agcttcgagc tggcggataa cggcatcatc tttacgccac agtctgacct
     1741 ggtgatcctg aactacatcg ccaactatat cattcaaaac aatgcgataa atcaggactt
     1801 cttcagcaaa cacgttaacc tgcgcaaagg ggcgacggac atcggctacg gtttacgtcc
     1861 gacccatccg ctggaaaaag cagcgaagaa tccaggttct gacgcctccg aaccgatgag
     1921 ctttgaagat tacaaagcct tcgttgccga gtatacgctg gaaaaaaccg ctgaaatgac
     1981 cggcgtgccg aaagaccagt tagaacaact ggcgcagctg tatgccgatc cgaacaagaa
     2041 agtcatctcc tactggacga tgggcttcaa ccagcatact cgtggcgtgt gggctaacaa
     2101 cctggtctac aacctgcacc tgctgaccgg caaaatttcc cagccgggtt gcggtccgtt
     2161 ctccctgacc gggcaacctt ctgcgtgtgg tactgcgcgt gaagtgggca cctttgctca
     2221 ccgtctgcct gcggacatgg tggtgaccaa cgagaaacac cgtgatatct gcgagaagaa
     2281 gtggaatatc ccgagcggca ccattccggc gaaaatcggt ctgcacgcgg tggcacaaga
     2341 ccgtgcgctg aaagacggca aactgaatgt ttactggacc atgtgtacca acaacatgca
     2401 ggccgggccg aacatcaatg aagagcgtat gccgggctgg cgcgatccgc gcaacttcat
     2461 catcgtctcc gatccgtatc cgacagtcag tgcgctggcc gccgacttga tcctgccgac
     2521 cgcaatgtgg gtagagaaag agggtgctta cggtaacgcc gaacgccgta ctcagttctg
     2581 gcgtcagcag gtacaggcac cgggcgaagc gaaatcggat ctctggcagt tagtccagtt
     2641 ctcccgccgc ttcaaaactg aagaagtgtg gccggaagag ctgctagcga agaaaccgga
     2701 actgcgtggc aaaacgctgt acgaagtgct ttatgccaca ccggaagtga gcaaattccc
     2761 ggtatccgaa ctggcggaag atcagctgaa cgatgaatcc cgcgagctgg gcttctatct
     2821 gcaaaaaggg ctgttcgaag agtacgcatg gtttggtcgc ggtcacggtc acgatctcgc
     2881 gccgttcgat gactaccaca aagcgcgcgg tctgcgctgg ccggtggtga acggtaaaga
     2941 aacgcagtgg cgttacagcg aaggtaacga cccgtacgtg aaagcgggcg aaggctataa
     3001 gttctacggt aaaccggatg gtaaagcggt gatcttcgcg ctgccgttcg aaccggcggc
     3061 ggaagcaccg gatgaagagt acgacctgtg gctctctacc ggacgcgttc tggagcactg
     3121 gcacactggc agtatgactc gccgtgtgcc ggaactgcac cgcgccttcc cggaagcggt
     3181 cttgtttatt cacccgctgg atgcgaaagc gcgcgatctg cgccgtggcg acaaagtgaa
     3241 agtggtttct cgccgtggcg aagtgatctc gattgttgaa acgcgcggtc gtaaccgtcc
     3301 gccacagggc ctggtgtaca tgccgttctt cgacgccgca cagctggtta acaaactaac
     3361 gctggacgcg accgatccgc tctcgaaaga gacggacttc aagaagtgcg cagtcaaact
     3421 ggagaaggtg taagccatta tgtcccggtc agcgaaacct caaaatggtc gccgccgctt
     3481 tctgcgcgat gttgttcgca cagcaggcgg gctggctgcc gtgggtgtgg cgctggggtt
     3541 acaacagcaa accgcacgcg catctggcgt gcggttgcgc ccgcccggag ccataaacga
     3601 gaacgccttt gccagtgcct gtgtgcgttg cggtcagtgt gttcaggctt gcccttacga
     3661 caccttaaaa ctggcgacgc tggcttctgg cctgtcggcg ggcacgccat attttgtcgc
     3721 acgggatatt ccttgcgaaa tgtgtgagga cattccgtgc gccaaagtgt gcccgagtgg
     3781 tgcgctggat cgtgagatcg aatcgatcga cgacgcgcgg atggggctgg cggtgctggt
     3841 ggaccaggaa aactgtctca actttcaggg gctgcgctgc gatgtttgtt atcgcgaatg
     3901 cccgaaaatt gatgaggcca tcaccctgga gctggagcgc aacacgcgta ccggtaagca
     3961 cgcccgcttt ctgccgacgg ttcacagcga cgcctgtacc ggctgcggta agtgcgaaaa
     4021 agtgtgcgtg ctggaacaac cggcaatcaa ggtgttaccg ctgtcactgg cgaaagggga
     4081 gttaggtcac cattaccgct tcggctggct ggaggggaac aatggcaaat cgtaaacgtg
     4141 acgccgggcg cgaggcgctg gtgaaaaaag gctggtggcg cagtcaccgt tggctggtgt
     4201 tacgtcgcct ttgtcagttc ttcgtgctgg ggatgttttt gagcggtccg tggtttggtg
     4261 tgtggatctt acacggcaac tacagctgta gcctgttatt cgacaccgtt ccgctgaccg
     4321 atccgctgat gacgctgcaa agtcttgcca gtggtcatct gcctgccacg gtggcgttga
     4381 ccggtgcggt gataatcact gtgctctacg ccctggcagg aaagcgatta ttttgcagct
     4441 gggtctgccc gctgaacccg attaccgact tagcgaactg gttacgcagg cgctttgacc
     4501 tcaatcagtc tgcgacgatc cctcgccaca ttcgctacgt gctgttggtg gtcattctgc
     4561 tgggatcagc cttaaccggc acgctcatct gggaatggat caaccccgtt tccctgatgg
     4621 ggcgtagcct ggtaatgggc ttcggcagcg gcgcgctgct tattctcgcg ctgtttttat
     4681 ttgatctact ggtcgttgaa cacggctggt gcgggcacat ttgtccggtt ggcgcgctgt
     4741 atggcgtgct gggtagcaaa ggtgtgatta ccgttgcggc cagcgatcgg cagaaatgta
     4801 accgctgtat ggattgtttt catgtttgcc cggaaccgca tgtgctacgt gccccggtgc
     4861 tggatgaaca aagcccggtg caggtcacca gccgcgattg catgacgtgc ggtcgctgcg
     4921 tggatgtctg ttctgaggat gtatttacaa taactacacg atggagttcg ggagcgaaat
     4981 catgaaaagc catgacctga agaaagcgct gtgtcaatgg acggcgatgc tggccctggt
     5041 ggtaagcggc gcggtttggg cggctaatgg cgtcgatttt agccagtcgc cggaagtatc
     5101 cgggacgcag gaaggggcca ttcggatgcc aaaagagcag gatcggatgc cgctgaacta
     5161 tgtgaatcag ccgccaatga tcccgcatag cgttgaaggt tatcaggtaa cgaccaatac
     5221 caaccgctgc ttgcagtgcc acggtgtcga aagctatcgc accactggcg cgccgcgtat
     5281 cagtcctact cactttatgg acagcgacgg caaagtgggc gcggaagtgg ctccgcgtcg
     5341 ttatttctgt ctgcaatgtc acgtaccgca ggccgatacc gcgccaatcg tggggaatac
     5401 ctttacccca tcaaaaggtt acgggaaata agaggttatt atgggaaatt ctgaccgtaa
     5461 gcctggtctg attaagcgcc tgtggaaatg gtggcgtacc cccagccgtc tggcgctggg
     5521 aacgctgctg ttgatcggtt ttgtcggcgg catcgtcttc tggggcggct ttaacaccgg
     5581 aatggaaaaa gccaataccg aagagttctg cattagctgc cacgaaatgc gtaacacggt
     5641 gtatcaggaa tacatggatt ccgtgcacta caacaaccgt agcggcgtcc gtgcgacctg
     5701 tccggattgt cacgttccgc acgagtttgt gccgaagatg atacgcaagc tcaaagcaag
     5761 taaagagctg tatggtaaaa tttttggcgt tattgacacg ccgcagaaat ttgaagctca
     5821 tcgtctgaca atggcacaga atgagtggcg gcgcatgaag gacaataact cgcaggagtg
     5881 ccgtaactgt cacaacttcg agtatatgga tacaaccgcc cagaaatcgg ttgccgcgaa
     5941 gatgcatgac caggcggtga aagatgggca aacctgtatt gattgccata aagggatagc
     6001 gcacaagctg cccgatatgc gtgaagtcga gccaggtttt taacagggtt attgcgtggg
     6061 tatgcttgaa gccagagagt tactttgtga gcgggatgaa cgaaccttat ttagtggctt
     6121 gtcatttacg ctgaacgcag gagagtgggt acaaatcacc ggtagcaacg gcgcggggaa
     6181 gacaacgctt ctccgtttgc tgacggggtt gtctcgccct gacgcaggcg aggttctctg
     6241 gcaagggcag cccttgcatc aggtacgcga cagctaccat caaaacctgt tatggatagg
     6301 ccatcagccg gggatcaaaa cccggctgac ggcgttagaa aatctgcact tttatcatcg
     6361 cgatggcgat accgcacaat gtctggaagc cctggcgcag gccgggcttg ccggattcga
     6421 cgatattccg gtaaatcaac tttctgccgg gcaacaacgc cgcgtcgctt tagcgcgtct
     6481 gtggctgacc cgtgccacgt tatggatcct cgacgagcct tttaccgcga ttgacgttaa
     6541 cggcgtcgat cgtctgaccc agcgtatggc gcagcatacg gagcaggggg ggattgtgat
     6601 tctgactacc caccagccgc tcaacgttgc tgaaagtaaa attcgccgca tttcactgac
     6661 gcaaacgagg gccgcatgat gttctggcgc attttccgtc ttgagctgcg tgtagcgttt
     6721 cgccatagcg ccgaaatcgc caacccgctg tggttcttcc tgattgtaat tacccttttt
     6781 ccgctcagta tcggtccgga gccgcaactg ctggcgcgta ttgcaccggg cattatctgg
     6841 gttgctgcgc tgctttcatc cttgctggcg ctggaacgac tgttccgtga cgatttgcag
     6901 gacggcagtc ttgaacaatt gatgttgttg ccgttaccct tgcccgccgt tgtgctggcg
     6961 aaggtgatgg cgcactggat ggtaaccggt ctgccgttac tcatcctttc gccactggta
     7021 gcaatgctac tgggaatgga tgtttatggc tggcaagtga tggcgctgac gctgctgctg
     7081 ggaacgccta cgcttggctt tctcggtgca ccgggtgtgg cgttgacagt gggacttaag
     7141 cgcggtggtg tgctgctcag catactggtg ttaccgctga ctatcccatt actcatcttt
     7201 gccaccgccg cgatggacgc ggcttccatg catttgcccg ttgacgggta tctggcaatt
     7261 ttaggcgcgt tgctggcagg caccgcgaca ttaagtcctt ttgcgacggc ggcagcgtta
     7321 cgaatcagca ttcaataacg ctggattact gatttttgtt cgagtctggt atcgaaacta
     7381 tgtggaaaac actgcatcaa ctggcgatcc caccacggct gtatcaaatc tgtggctggt
     7441 ttataccgtg gctggcaatt gccagtgtgg tcgtgcttac cgtcggctgg atctggggat
     7501 tcggctttgc tccggctgat tatcagcagg gaaatagcta ccgcattatc tacctgcatg
     7561 tgcctgcggc gatctggtcg atgggcattt atgcatcaat ggcagtggca gcgtttattg
     7621 gccttgtctg gcagatgaaa atggccaacc tggcggtggc ggcgatggcc cccattggtg
     7681 ccgtgtttac ctttattgcc ctggttaccg gctctgcatg gggaaaaccg atgtggggca
     7741 cctggtgggt atgggatgca cgtctgactt ctgaactggt gctgctgttt ttgtatgtgg
     7801 gtgtgattgc cctgtggcac gccttcgacg accgccgtct ggcgggccgt gcggcaggta
     7861 tcctggtgct gattggcgtg gtgaatctgc cgattattca ttactccgtg gagtggtgga
     7921 acaccctgca tcagggatca acgcggatgc agcaaagtat cgatccggcg atgcgttcgc
     7981 cgctgcgctg gtcgattttt ggcttcctgc tcctgtctgc cacgctgacg ctgatgcgga
     8041 tgcgtaattt gattttgctg atggaaaaac gccgtccgtg ggtgagtgaa ctgatactga
     8101 aaagaggccg taaatgaccc ctgcatttgc ttcctggaat gaatttttcg caatgggcgg
     8161 ttacgccttt tttgtctggc tggcggtggt gatgaccgtt attccgctgg tggttttggt
     8221 cgtgcactcg gtgatgcaac atcgcgcaat tctgcgtggt gtggcgcaac agcgggcgcg
     8281 tgaggcgcgt ttacgtgctg cgcaacagca ggaggctgca tgaatattcg ccgtaaaaac
     8341 cgcttgtgga ttgcctgtgc cgtgttggca gggctggcgc tgactatcgg tctggtgcta
     8401 tatgcgctgc gctcgaatat cgatctcttt tatacgccgg gggaaattct ctacggcaag
     8461 cgtgaaactc agcaaatgcc ggaagtcggt cagcgtctgc gcgttggcgg gatggtgatg
     8521 ctgggtagtg tgcagcgcga tcccaattcg ctgaaagtga ccttcactat ttacgatgct
     8581 gaaggctcag tggatgtctc ttacgaaggc attttgccgg atctgttccg tgaagggcag
     8641 ggcgttgtgg tgcagggcga gctggaaaaa ggcaatcata tcctcgcgaa agaagtgctg
     8701 gcgaaacacg acgaaaacta cacgccgcca gaagttgaga aagcgatgga agctaaccac
     8761 cgtcgcccgg cgagtgttta taaggaccca gcatcatgat gccggaaatt ggaaacgggc
     8821 tgctgtgtct ggcgttaggg attgcgctgc tgctgtccgt gtatccgcta tggggcgtgg
     8881 cgcgcggaga tgcgcgcatg atcgcgtctt cccgcttgtt tgcctggctg ctgtttatgt
     8941 ctgtggctgg cgcatttctg gtgctggtca atgctttcgt ggtcaacgac tttaccgtca
     9001 cctatgttgc cagctactcc aatacccagc ttccggtgtg gtatcgcgtg gcggctacct
     9061 ggggcgcgca tgaaggctcg ctactgctgt gggtgctgct gatgagcggc tggacctttg
     9121 cggtggcgat ttttagtcag cgtattccgc tggatattgt ggcccgcgta ctggcgataa
     9181 tggggatggt cagcgtcggc tttttgctgt tcattctctt tacctctaac ccattctcac
     9241 gcacgctgcc gaacttcccg attgaagggc gcgatcttaa cccgctgtta caggacccgg
     9301 ggctgatctt ccatccgcct ctgctttata tggggtacgt gggtttctcg gtggcgtttg
     9361 cttttgccat tgcttctttg ctgagcgggc gtctggacag cacttatgcg cgttttactc
     9421 gcccgtggac gctggcggcg tggattttcc tgacgctcgg cattgtgctc ggttccgcat
     9481 gggcctatta cgaactcggc tgggggggct ggtggttctg ggatccggta gaaaacgcct
     9541 cgtttatgcc gtggctggtg gggactgcgc tgatgcactc actggcggtc actgaacaac
     9601 gcgccagctt caaagcgtgg acattactgc tggcaatcag tgccttctcg ttgtgtctgt
     9661 tggggacttt cctcgtgcgt tccggcgtgc tggtatcggt acacgcgttt gcgtctgatc
     9721 cggcgcgcgg tatgtttatc ctcgccttta tggtgctggt gattggaggt tcgctgctgc
     9781 tgtttgccgc gcgtggacac aaagttcgct cacgcgtaaa caatgcgctg tggtcgcggg
     9841 aatctctgct gttagcgaac aacgttttgc tggtcgccgc gatgctggtg gtgttgctgg
     9901 ggacgctgct gccgctggtg cacaagcaac tgggactggg cagtatttcg attggcgaac
     9961 cgttcttcaa caccatgttt acctggctga tggtgccgtt tgcgctactg ctgggtgtcg
    10021 gtcctctggt gcgctggggg cgcgatcgcc cacgtaaaat ccgcaattta ttgattatcg
    10081 ccttcatctc aacgctggtg ctgtctctgc tgttgccgtg gctgtttgag agcaaagttg
    10141 tggcgatgac ggtgctcggc ctggcaatgg cctgctggat tgcggtgctg gcaattgcgg
    10201 aagctgcgct gcgtatttca cgcggcacga aaaccacctt cagttattgg gggatggtgg
    10261 ctgctcacct ggggctggca gtgacaattg ttggcattgc ttttagccag aactatagcg
    10321 ttgagcgtga tgtgcgcatg aagtccggcg atagcgtcga tattcatgaa tatcgcttca
    10381 ccttccgtga tgtcaaagag gtgactggcc cgaactggcg tggcggtgtg gcgactatcg
    10441 gcgtaacgcg agagggtaaa ccggaaacag tgctgtatgc ggaaaaacgt tattacaaca
    10501 ctgccgggtc gatgatgacc gaagcggcga ttgacggtgg catcacgcgt gacctgtacg
    10561 ccgcgctcgg tgaagagctg gaaaacggcg cgtgggctgt gcgtctttac tacaaaccat
    10621 ttgttcgctg gatttgggcg ggcgggctga tgatggcgtt gggcggactg ctgtgtctgt
    10681 ttgatcctcg ctatcgtaag cgcgtgagtc cgcaaaaaac tgcgccggag gctgtatgaa
    10741 gcgcaaagta ttgttaattc cgttgattat cttcctggcg attgccgcgg cgctgctgtg
    10801 gcagctggcg cgtaatgccg aaggggatga tccgaccaat ctggaatcgg cgctcattgg
    10861 caagccagtg ccgaagtttc gcctcgaatc actggacaat ccggggcagt tttatcaggc
    10921 ggatgtgctg actcagggca aaccagtgct gcttaacgtc tgggcgacct ggtgtccgac
    10981 ctgccgtgcg gaacatcaat atctgaatca gctttctgcg cagggcatcc gcgtagtcgg
    11041 catgaactat aaagacgatc gccagaaggc aatcagctgg ctgaaagagc tgggcaatcc
    11101 ttacgcgcta agcctgtttg atggcgatgg catgttaggg ctggatctcg gtgtctatgg
    11161 cgcgccagaa acgttcctta ttgacggcaa cggcatcatt cgctatcgcc atgcgggcga
    11221 tctcaatcct cgcgtctggg aagaagagat caagccgctg tgggagaaat acagtaagga
    11281 ggccgcacaa tgaggttttt attgggcatg ctgatgctga tgatctccgg ctcagcgctg
    11341 gcgaccatcg acgtgttgca gtttaaagat gaagcacagg aacaacagtt ccgtcagctc
    11401 actgaagaac tgcgctgccc gaaatgccag aacaacagca ttgccgattc caactcgatg
    11461 attgccaccg acctgcgcca gaaagtgtat gaactgatgc aggaaggtaa aagtaaaaaa
    11521 gagattgtcg attatatggt ggcgcgttac ggcaacttcg tcacttacga tccgccgtta
    11581 acgccgctga ccgtgctgct gtgggtgctg ccagtagtag ctattggcat tggcggttgg
    11641 gtcatatacg cccgctcgcg gcgtcgggta cgcgtggtgc cggaagcgtt tcctgaacaa
    11701 agcgtgccgg aaggtaagcg tgccggatat gtcgcttatt tgccgggcat tgtggtggcg
    11761 ttaattgtgg ctggcgtcag ctactaccag accggaaatt atcaacaggt gaaaatctgg
    11821 cagcaggcca cggcacaggc tccggcgttg ctggacaggg cgctggatcc gaaagctgat
    11881 ccgctcaacg aagaagagat gtcgcgcctt gcgctgggga tgcgtactca actgcaaaaa
    11941 aatccgggag atatagacgg ctggattatg ttgggccgcg ttggcatggc gctgggtaac
    12001 gccagtatcg ccaccgatgc atacgctact gcgtatcgcc tcgatccgaa aaacagtgat
    12061 gccgcactgg gttatgctga agcgttgaca cgttcatctg atcccaacga caaccgcctc
    12121 ggcggcgaac tgctacgtca gctggtgaga acggaccata gcaatatccg tgtgctaagc
    12181 atgtatgcgt ttaatgcctt tgagcagcag cgatttggcg aagccgttgc cgcgtgggag
    12241 atgatgttga aactcttacc tgccaacgat actcgccgtg cggtgattga acgtagtatc
    12301 gcgcaggcga tgcaacattt gtcgccgcag gagagtaaat aaaaagaggc ccgatggtga
    12361 tgccatcggg ctatttttat tgtgccccgc gttgttgcag gaacagaatg gtggccgcca
    12421 cgcgtgagcg gacattgagt ttacgcagca gatttcgaat atgaactttt actgtctgct
    12481 cggaaatatt caacacagag gcaatctgtt tatttgacag cccctgtgcc agctcgtgca
    12541 gaacatccag ctcgcgctcc gtcagcacgc tgaagggatc ttcttccgcg ccaaacattt
    12601 cacgttcacg taagtactga ttgacgcgtt cgctaaagac tttgctgcct ttcgctccgg
    12661 cacgaatcgc ttccagcaac acttccgggt cgctgtcttt cagcagataa ccgtctgcgc
    12721 ctgcgtctat cagcgcaaag acatcgctgg aggcatcgga tacggtcagg ataataattt
    12781 gcgcggtaac gccatccctg cgcaaggcat tgagggtatc caggccactc atacctttca
    12841 tattgagatc cagcaagatc acgtcgatat ccagtctatt cgccagatcg atcgcgctcg
    12901 cgccgtcgcc cgcttcggcg accacttcaa agccaggatc aagctccagt aactgacgaa
    12961 caccgcgtcg cataagtgga tgatcatcca caatcatcac ctgaaaaggc gttgcttcag
    13021 gcatagtagt ctcctgaggt tttattagaa tcattatgtt tgagcgtagc gttggtaacg
    13081 ctaccaggcg cgtgaatgtt accccatttt atgtatggac atgtagtgaa aactacaacc
    13141 ttgccgttcg ctttggcatc attttcatta taaagacgga attttgcatc agcttctcat
    13201 caagaaaaac aactaaaaaa tcaatcacaa tacttgtgca tttctttaaa cagtttttaa
    13261 aaactgactc cgggtatgga gctatgggta ttttctgtac ccaatgcttt taacagcaat
    13321 taatttcata ggatgaaagc tcaatgcatc aatctggttc tgtttctctt tgtcgttccg
    13381 caatatctgt tctggtggct acagcgttat attcacccat agcattggca tcaacagttg
    13441 agtatggtga gacagttgat ggtgttggcc tggaaaaaga tatccagctg gtttatggga
    13501 ccgccaataa tacgaaaatc aatcctggcg gagaacagca tataaaagaa tttggtgtaa
    13561 gtagtaatac tgaaattaac ggcgggtatc agtacattga aatgaatggc actgcagaat
    13621 actcagtatt aaatgatggt tatcaaattg ttcaaatggg tggcgcggta aaccagacta
    13681 cgctcaataa tggagtgcta caggtttatg g
//
LOCUS       DAZRUF010000103        13643 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZRUF010000103 DAZRUF010000000
VERSION     DAZRUF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.103"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..660
                     /locus_tag="ACHXOL_002395"
     CDS             <1..660
                     /locus_tag="ACHXOL_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR6809357.1"
                     /translation="KLLTQEDREFIFTNYRGDGIGATGAFFTPEILAWDFVLDADCTG
                     DCIELCAGIGRLSYYQYLRNKPTHITCVELNPEYVMIGKRVLPEAEWITGNALQYSPD
                     RFYDVAYGNPPFGKISTSKAYTGRYKGSEFEYKVIEHASTFASYGAWIVPQGSAGFVY
                     SGVHCYERRESCKYQKFVKDTGYELHPGVGIDTSIYRDEWNGTKVICESVLVDDDGTN
                     L"
     gene            1153..1899
                     /gene="mipA"
                     /locus_tag="ACHXOL_002396"
     CDS             1153..1899
                     /gene="mipA"
                     /locus_tag="ACHXOL_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR6809358.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTILAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            1989..2843
                     /gene="yeaE"
                     /locus_tag="ACHXOL_002397"
     CDS             1989..2843
                     /gene="yeaE"
                     /locus_tag="ACHXOL_002397"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR6809359.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(2894..3778)
                     /locus_tag="ACHXOL_002398"
     CDS             complement(2894..3778)
                     /locus_tag="ACHXOL_002398"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR6809360.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(3862..4857)
                     /gene="gapA"
                     /locus_tag="ACHXOL_002399"
     CDS             complement(3862..4857)
                     /gene="gapA"
                     /locus_tag="ACHXOL_002399"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR6809361.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5199..5612
                     /gene="msrB"
                     /locus_tag="ACHXOL_002400"
     CDS             5199..5612
                     /gene="msrB"
                     /locus_tag="ACHXOL_002400"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR6809362.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            5654..5926
                     /locus_tag="ACHXOL_002401"
     CDS             5654..5926
                     /locus_tag="ACHXOL_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR6809363.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(6087..6728)
                     /gene="pncA"
                     /locus_tag="ACHXOL_002402"
     CDS             complement(6087..6728)
                     /gene="pncA"
                     /locus_tag="ACHXOL_002402"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR6809364.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(6739..7755)
                     /gene="ansA"
                     /locus_tag="ACHXOL_002403"
     CDS             complement(6739..7755)
                     /gene="ansA"
                     /locus_tag="ACHXOL_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR6809365.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(7922..9778)
                     /gene="sppA"
                     /locus_tag="ACHXOL_002404"
     CDS             complement(7922..9778)
                     /gene="sppA"
                     /locus_tag="ACHXOL_002404"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR6809366.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            9939..10490
                     /locus_tag="ACHXOL_002405"
     CDS             9939..10490
                     /locus_tag="ACHXOL_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR6809367.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            10607..11650
                     /gene="selD"
                     /locus_tag="ACHXOL_002406"
     CDS             10607..11650
                     /gene="selD"
                     /locus_tag="ACHXOL_002406"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR6809368.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            11655..13616
                     /gene="topB"
                     /locus_tag="ACHXOL_002407"
     CDS             11655..13616
                     /gene="topB"
                     /locus_tag="ACHXOL_002407"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR6809369.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         3457 a         3320 c         3579 g         3287 t
ORIGIN      
        1 aagctcctaa cacaagaaga tagggaattc atattcacca attatagggg ggatgggata
       61 ggggctacag gtgccttctt cacacctgaa attttagcat gggattttgt acttgatgct
      121 gactgtactg gtgactgtat tgaactatgt gctggtatcg gtcgcctcag ctactaccag
      181 tacttaagga ataaaccaac gcatattaca tgcgtggaac tcaatcctga gtacgtaatg
      241 atcgggaaga gggtactacc agaggcagaa tggatcactg gtaacgcact acagtactca
      301 ccagaccgtt tctatgatgt tgcctatggt aatccaccct ttgggaagat cagtacctca
      361 aaagcgtata caggccgtta taagggtagt gaatttgaat acaaggtgat tgaacatgca
      421 agtacctttg cgtcctatgg tgcatggata gtaccgcaag gttccgcagg ctttgtctac
      481 agtggggtac attgttatga gcgtagagaa tcatgtaagt accagaagtt tgtgaaggat
      541 actggctatg agctacatcc aggtgtaggc atcgatacaa gtatctacag ggatgaatgg
      601 aatggtacta aggtgatatg cgaatcggtg ttagtagatg atgacggtac aaatctgtaa
      661 ctaaattagt taagaaatca taaagatagt agtacttgca aatgttccca ttgatgaagt
      721 tatgcgtcct ttcgtttttt agcacaggtt aagagccgtt catatgaata gtagaggctc
      781 ccggaagagg taaactgaac gacgtctctt gctaaaacac agcaactctc atgccatttt
      841 atcgacgatg agaacaacgt gcccggtgat acaccttcaa aattaagcgt agatggcaaa
      901 tggaaaactt gcctgcacgg ctttcttaat ttcaatggca tatcaacaac tcatccataa
      961 attttgcata attaatgtaa agacaaggct cgccagtaac gctaaattca tttggctgta
     1021 agcgcggtgt catccgcgtc aggaaaatta aacagttact ttaaaaaatg aaaacgtaaa
     1081 aaggttgggt ttcgatgtat tgacgggtaa actttgtcgc ccgctaaaca tttgtttaag
     1141 gaatgattaa ttgtgaccaa actcaaactt ctggcacttg gagtgcttat cgcaacgtct
     1201 gcaggcgtag cgcacgctga aggtaaattt tccctgggcg caggcgtcgg tgtcgttgaa
     1261 cacccatata aagattacga taccgatgtt tacccagtac cggtaatcaa ctatgaaggc
     1321 gataacttct ggttccgtgg cttaggtggt ggttactacc tgtggaatga cgcaacggac
     1381 aaactttcaa ttaccgctta ttggtcgccg ctttacttca aagcgaaaga cagtggcgat
     1441 caccaaatgc gtcacctgga tgaccgtaag agcaccatga tggctggtct gtcttatgct
     1501 cactttaccc agtacggtta cctgcgtacc atcctggctg gcgataccct ggataacagc
     1561 aacggcattg tctgggatat ggcctggttg tatcgttaca ccaacggcgg cctgacagtg
     1621 accccgggta tcggtgtgca gtggaacagc gaaaaccaga acgaatacta ttatggcgta
     1681 tcgcgcaaag agtccgctcg cagcggtctg cgtggctata acccgaacga cagctggagc
     1741 ccttacctgg agctgagcgc cagctacaac ttcctcggcg actggagtgt ttacggtacc
     1801 gcgcgctata cccgtctgtc tgatgaagtt actgacagcc cgatggtgga taaatcctgg
     1861 actggcctga tttctaccgg gatcacctac aaattctgat aatgcatctt tccagggcat
     1921 tttgtgcata aaaacagggc gtcacgccct gttttgcatt acggtggtgc aatacctggg
     1981 aggaaacaat gcaacaaaaa atgattcaat ttagtggcga tgtctcactg ccagccatag
     2041 ggcagggaac atggtatatg ggcgaagatg ccagtcagcg caaaacagaa gttgctgcac
     2101 tacgcgcggg cattgagctc ggtttaaccc tcattgatac cgccgaaatg tatgccgatg
     2161 gcggtgccga aaaggtggtt ggggaagcat taaccggtct gcgtgataac gtctttctcg
     2221 tctctaaagt ctatccgtgg aatgctggcg ggcaaaaagc gataaatgca tgcgaagcca
     2281 gtttacgccg tctcaatact gattatctcg atctctactt attacacagg tctggcagtt
     2341 tcgcttttga agagactgtt gcagcgatgg aaaaattgat cgcccaggga aaaatccgcc
     2401 gctggggcgt ttctaacctt gattatgctg atatgcagga actctggcag ctgccggggg
     2461 gaaatcagtg tgccactaat caggtgcttt accatctcgg ttcacgaggg attgagtacg
     2521 atctactccc ctggtgccag caacagcaga tgccggtgat ggcttacagt ccattagccc
     2581 aggccgggcg gttgcgcaat ggactgttaa aaaacgcggt agtcaacgaa attgcacatg
     2641 ctcacaatat cagctcggca caagtattgt tggcgtgggt aatcagccat cagggtgtga
     2701 tggcgattcc aaaagcggcc acgattgccc atgttcaaca gaatgcggct gtgcttgagg
     2761 tcgaactttc gtcagcggaa ttaaccatgc tggataaagc gtatccggca ccaaaaggaa
     2821 aaactgcgct ggatatggtg tgatgttgat acgtgccgga tgcatcgcac ccggcacaca
     2881 acattaacgc aaattaacgt ttcgcaacgc gaatggattg cgccagatgt gcaggtttct
     2941 ctttggtgac tttttgcgtt tctgaagcgt aagccgtttc tacacaaaca aatgttttgt
     3001 agccatcatc cggcatatcg cccatgctaa ttgaaagcgc cggtcccggg ttccagccta
     3061 caacgttcag atgatgctgg tggcctacgg cgataatacg attcagcgct tcatcattaa
     3121 tcacgctgca atcttgcgga ttcagataca cgcgatcggt acggtcaggg aaggtctgaa
     3181 taccgtcggt cagtacatct tctttcgcgt cattcacttt atcaatgaag cgatcgccca
     3241 gcccactgac gcttacctta gcgatatcac ccacgttaaa gtaggtatgc agggcagagg
     3301 tggtttcaaa ttcgccatgt gattcaagat cgatttcaca ggttttaccc acgcggaaat
     3361 gcgctaacag cgtaaagtcg tgcggacaga attttttcgt ctcttcgctt tgcgtcaatt
     3421 caaaagtcag cgctacgcca tcagcatctt cattatgtga tttcagcgtc cacggcaggt
     3481 tgcgggcaaa accgtgcgca ggcagacctt gttgtgccgc cggaccaaac cacggccagc
     3541 aaaccggtac gccaccgcga atagcgacgc catttttgaa cggtgtgttg ttgctcaacc
     3601 acagaacttc ttcttcaccc gcaggtttcc acgagagaag gtgtgcgccc tgtaatgcaa
     3661 aagaggcttt tacttgggga tgatcgacca caatgaggtc cagttcatcc agtttacgac
     3721 gggagaggac aggggagatt tgttcgatga ccggaagggc aaaaattttc ttaatcatga
     3781 cgcagtcctt taacttcatt ttatcaggta aaaaaaagag cgaccgaagt cgctcttttt
     3841 agatcacagt ttcatctcaa cttatttgga gatgtgagcg atcaggtcca gaactttgtt
     3901 ggagtaaccg gtttcgttgt cgtaccagga taccagtttc acgaagttgt cgttcagagc
     3961 gataccagct ttagcatcga acacggaagt gcaaacttcg ccgttgaaat cggtagatac
     4021 tacgtcatct tcggtgtagc ccagaacgcc tttcatttcg ccttcagcag cggctttaac
     4081 ggcagctttg atctgctcgt aagttgcagc tttttccaga cgaacggtca ggtcaactac
     4141 ggatacgttc ggggtcggaa cgcggaacgc cataccagtc agtttgccat tcagttctgg
     4201 cagtacttta cctacagctt tagcagcacc ggtagaggac gggatgatgt tctgggaagc
     4261 gccgcggccg ccgcgccagt ctttgtgaga cgggccatca acggttttct gagtagcggt
     4321 agtagcgtga acggtggtca tcagaccttc gatgatgccg aagttatcgt tgataacttt
     4381 agccagcgga gccaggcagt tggtggtgca ggaagcgttg gaaacgatgt cctggccagc
     4441 atatttgtcg aagttagcgc ctttaacgaa catcggagtg ttgtctttag acggaccagt
     4501 cataaccact ttcttcgcac cagcggtgat gtgtttacga gcagtttcgt cagtcaggaa
     4561 cagaccagtt gcttcagcga caacgtcaac accaacttcg tcccatttca ggttagccgg
     4621 atcacgttca gcggtaacac ggattttttt accgttaacg atcagatgac cgtctttcac
     4681 ttcaacggta ccgtcgaaac ggccgtgagt ggagtcatat ttcagcatgt atgccatgta
     4741 atcagcgtct aacaggtcgt tgattgcaac gatctcgatg tcagaacgtt tctgagcagc
     4801 acggaaaaca atgcgaccga tacggccaaa accgttgata cctactttga tagtcatata
     4861 ttccaccagc tatttgttag tgaataaaag gttgcctgta aaattacaaa aaccttacgc
     4921 agcgtcaagc ggaatcgtgt caatcattgc gacaaatcaa tcctgtgcct aagcattacg
     4981 cgactgactc gcctcactct tcctttgggc ttgaaaccac atggggtcgg cgccccgaat
     5041 tttaaagggc aattacgata aaaatgtgat tttcatcacg atttaggtgc agccatttcg
     5101 tcgagatcaa gtttaggaca aaattttgcc agttggtgtt aatgttttgt tagaatcagt
     5161 cacgtaatgt gagcacgatt aaagtgagat gtgagcaaat ggctaataaa ccttcggcag
     5221 aagaactgaa aaaaaatttg tccgagatgc agttttacgt gacgcagaat catgggacag
     5281 agccgccatt tacgggtcgt ttactgcata acaagcgtga cggcgtatat cactgtttga
     5341 tctgcgatgc cccgctgttt cattcccaaa ccaagtatga ttccggctgt ggttggccca
     5401 gtttctacga accggtgagt gaagaatcca ttcgttatat caaagacttg tcacatggaa
     5461 tgcagcgcat agaaattcgt tgcggtaact gtgatgccca tctggggcat gtcttccctg
     5521 acgggccgca gccaacgggc gaacgttatt gtgttaactc tgcttcttta cgcttcactg
     5581 atggcgaaaa cggcgaagaa atcaacggtt gaaaaaacga ttcagcaact tattccacag
     5641 gagcggaaaa attatgaatc ttgatgacat tatcaacagc atgacgcctg aggtatacca
     5701 gcgtttgtcg accgccgttg aactggggaa atggcccgat ggcgttgcgt tgaccgagga
     5761 acaaaaagcg aactgcctgc aactggtgat gctgtggcaa gctcgccata ataccgaagc
     5821 acagcatatg acaattgaca caaatggtca gatggtcatg aagagcaaac agcagttaaa
     5881 agaagatttt ggtatctcag caaagcctat tgctatgttt aagtgaatga gttatctagt
     5941 atttaagcct gttattaagg gagggcaaat atacttgtta ggacgtcgtg ttttagctgc
     6001 aagtaggtta aatgcaacac tgacaagtat tatctggcct acaatgtcat tgaggaaatg
     6061 taggccagag atgaattcaa ctaatattac ccctgtgtct cttcccagtc tgccagcgta
     6121 tatagcgttg ccccagctgc tgacatctcc ataaacgcgt gcgcactgtc ctggggctgg
     6181 atattcacgc cacgacaacc atcggtaatc acatttacct tataacctaa ctgtaacgcg
     6241 tccagcacgg taaacttcac gcaatagtca gtagccaggc ccataacgat caattcatcg
     6301 atttcatgat cgcgtaacca gtcatcgagc gaggttttct gccgacggcc gttatcaaaa
     6361 aaggcactgt aactgtcaac taaaggattt tcgcctttat ggaacaccgc tgcgatcgct
     6421 ttttggttca gtaacggatg taattgtgcg ccttcactgt tctgcacaca gtgatctggc
     6481 cagaaggttt gtggcaaacc gtcgagttgg cctggcgtat aaggctctac accgtgctga
     6541 ctggcaaaac tgccgtgatt cgccgggtgc cagtcctgac tggcgataac cgcttcaccg
     6601 cgcgactggc accagtcaat caggcggtta gcgacatcca ccgtactgtc accttccggc
     6661 acggcgagcg cgccaccagc acagaaatca ttttgtaaat cgaccagtaa cagggcgcga
     6721 gggggcatta cagtctcctt aatcatccgg cgtcagttcg ccgcgcaggt tttggctcat
     6781 ggccttgcga atggtttcag tatccagttc ctggctcagc aggtaatgca gtttggttag
     6841 cgtggcttct acagtcatat ctgcaccgcc aataacgccg gcatgggcga gggcgttacc
     6901 ggtggcataa ccacccatat tcactttacc ggacatacat tgtgtcaggt tgaccaccac
     6961 aataccgcga tcgctggctt cttgtaattc ctgcaggaag gctttgtttt gtggcgcatt
     7021 acccacgcca taggagcgca gaatcaatgc tttcaccggt tggcgcagaa aattgcgcac
     7081 gacgtcagca gaaatccctg gataaatcgt cactacgcca attggttgtg gcgtgattgg
     7141 atgaacgatt aattcccctt caccgtgcgg ggcgggtggc gtattcaaac gacgaatatg
     7201 gataccagct tccagtaacg gaggaaggtt tggagaggca aacgcatcaa aaccatcggc
     7261 atgggctttg gtagtgcggt tgccgcgata caatcggtta ttgaaaaaga gcgttacttc
     7321 gttgatcgga taattcgccg caacgtacaa cgcattcagc agattaattt gtccgtcaga
     7381 gcgtaactca gccagcggga tttgtgaccc tgtcacaatg accggtttac cgagattctc
     7441 gagcatgaac gacagcgcag aggcagtata cgccatcgtg tcggtgccgt gcaggatgac
     7501 aaaaccatca tagtcgtcat agtgcgcttt aatatcttca gcaatatgct gccagtcttc
     7561 tggcgtcata tcagaagaat ccatcagcgg cgtatattca tgaatggtga aatctggcat
     7621 ctccgggcga tggaattccg gcatcagcgc cagttggcgt tgtagatgac ctgacaccgg
     7681 aatataaccc tgctcggaac gctgcatccc gatggtcccg cccgtgtagg cgacgtaaat
     7741 tgatttcttt tgcatgatat tgatatgaat gatatcgaaa gagcaaaagt atatacgtga
     7801 ggcaggagga gcgcacctga tactatgcgg ataaggaaat atggggcgag acagcgggaa
     7861 gaggattcgt cgacgtaaaa aataatgtgg ccgacgatct gtcggccact cagtacaaga
     7921 cttaacgcac gttggcgcag gtcaggcaaa acgcataacg gttttgtggg tcgttaaacg
     7981 cggccagctt gtcgctttca cttttaacgg tagaggctac cgaggcaagc ggtgcaggta
     8041 acatggcctg gaacgcatct ggcaacattg cccggacaga accagacatg ttgtccatca
     8101 ctttgtcgaa gaaggtcggt tcatcaacgt agtattccag atgccactgt ttcacttttg
     8161 ccagctctgc tgctttggcg accgcatcat cgaaatcccc gagactatcg accagcccgt
     8221 tagcttttgc atcctgaccg gtccagacgt ggccctgggc gattttatca atctgctccg
     8281 gcgtcgaatg acgcgcatca gcaaccagcg tgataaagcg tttatagcca ttctcaatgc
     8341 ttaattgcat catctgctgc gcttccggcg gcagtgccct ggtgatagaa acatccgcca
     8401 gcggtgaagt tgagacacca tcagtatgaa caccaatcga atccagacta ttttctacgg
     8461 tggtgatcac gccgaagata ccgatagaac cggtcagggt gctggggtta gccacaatgt
     8521 aattagctgg cgtggaaatc cagtaaccac cagatgccgc catgccgccc atcgatacaa
     8581 ccacaggctt acccgctgcc cgggctgctg ccagttcagc gcgaatcact tcagacgcgg
     8641 taacgctgcc gcctgggcta ttaacacgca ggacaatcgc tttcactttc gggtcaaggc
     8701 gagcgtcgcg gatttgtgcc gcagtggtat caccgccaac attcccctga gtttcctcgc
     8761 catccataat tgcgccatta gcgaagacga caccgatgct gtcaccggta tctgccggcg
     8821 ttttcaatgc gtaatcgtaa taactgatgg cgcgataatt tttatcagtc ttactccagc
     8881 cgaactcttt ggttagagct ttttcgattt cggcactcga tgccagtgca tcgaccagct
     8941 tgttttccag tgcatatttc gcggtatcgc caccggtttt ggttaaaccc tcaagcaacc
     9001 cttgcgcgcc agggaatacc tgctgagcag ggatctgccg gttagcggca acagtattca
     9061 gatagttttg ccacagctca ccaatccagc ggctgtcagc ttcgcgggct gccggtgaca
     9121 tgtcatcacg aataaacggt tcaacggcag atttatacgt acccacgcgg aacacatggg
     9181 tggaaacttt cagcttatcc agcaacgatt tgtagtacag accgttggta gcaaaaccgt
     9241 gcaaatccac cacgccttgc ggtgacagcc aaattttatt ggcgaaactg gcgagataat
     9301 attgcccctg gctgtagttc tcgccaacgg cataaaccgg tttcccgctg tcacgaaact
     9361 ctttcagggc tttgccgatg tactgcatag acggctggtc gccgcctgcg aagtttttca
     9421 gatccatcac gataccggtg atattgcggt cgtccttcgc ctggcgaatg gtgttgacga
     9481 tatcaaacag tgagttttcc tgcagacgat cggaactggc accaagcagc tggcggctta
     9541 atttactaaa ccgctgagaa ctgtcgggtt tatcgacgat cacaccagaa atgtccagca
     9601 gcagtgcgcc acgactggcc gtttctttcg aatcaccacc actgacctgc atccagatcc
     9661 ccacaccaac cagtacgagg aaaataaaga acaggttaag taccatttca cggacgaaat
     9721 tcagcagacg ccacgtccat ttaaaaaatc cggcaataaa tcgccaaagg gttcgcatgt
     9781 attctcccaa cttaaggtca cacctgtcac aggcgcaata gatgtggtta tcgtaatgac
     9841 ccaaccggca cttgtcagca ggaattgcgg actgtgctgt aacaaaatcc actctcgtgt
     9901 taattttgtg aataaatatc acgacaggag ttaatcaaat ggatgcactc gaactattga
     9961 tcaatcgccg tagcgcctcc cgcctggctg aacccgcgcc aacgggtgaa caactgcaaa
    10021 acatcctgcg tgcgggtatg cgtgcgccgg accataagtc catgcaaccg tggcattttt
    10081 ttgtgattga aggggaaggg cgcgagcgtt tcagcgccgt actggaacag ggggcgattg
    10141 ctgccggtag tgatgacaaa gctatcgaca aagcccgtaa tgcgccgttc cgcgcaccgc
    10201 tcatcatcac ggtggtggcg aaatgcgaag aaaatcataa agtcccgcga tgggaacagg
    10261 aaatgtctgc cggatgcgcg gtcatggcga tgcaaatggc agccgttgcc cagggatttg
    10321 gcggcatctg gcgcagtggc gcattaactg aaagtccggt agtgcgtgaa gcattcggtt
    10381 gccgtgagca ggataaaatt gtcggttttc tctacctcgg cacgccgcag ttgaaagcat
    10441 ctacgtcgat taacgtcccg gacccgacgc cgtttgtaac ttatttctga taattgtcgc
    10501 gaaactgtct ggattgtgag caaaggcgca ggaattcaga cactctcact tatcacttca
    10561 cggaatgagg gttaccatac ccggattgca ttgacaggag atgtccatga gcgagaactc
    10621 gattcgtttg acccaataca gccacggagc tggttgcggc tgtaaaattt ccccaaaagt
    10681 gttggaaacc atcctgcaca gtgagcaggc gaagtttgtt gatccgaatt tgcttgtggg
    10741 taatgagacc cgcgacgatg cggcggtgta cgatctgggc aatggcacca gcgttatcag
    10801 taccaccgac ttctttatgc cgatcgttga taatcctttc gattttggcc gcattgcggc
    10861 gactaacgcc atcagcgata tcttcgcgat ggggggcaaa ccgattatgg cgattgcgat
    10921 cctcggctgg ccgattaaca aactttcccc ggaaattgcc cgcgaagtaa ccgaaggtgg
    10981 acgctatgca tgccgtcagg cgggtattgc gctggctggc ggtcactcca tcgatgcgcc
    11041 ggagccgatt tttggtctgg cggtaacggg gatcgtaccg accgagcggg tgaagaaaaa
    11101 cagtaccgca caagccggat gcaaactgtt cctgacgaaa ccgctgggga tcggcgttct
    11161 taccacggct gagaaaaaat cactgctgaa accagaacat cagggactag cgacggaagt
    11221 gatgtgccgg atgaacatcg caggcgcgtc ctttgccaac atcgaaggcg taaaagcgat
    11281 gaccgacgtt acgggttttg gtctgctggg ccatttgagc gaaatgtgtc agggcgcagg
    11341 tgtgcaggcg cgcgttgact atgacgcgat cccaaaattg ccgggggttg aagagtacat
    11401 taagttgggc gcagtgcctg gcggcaccga acgtaacttt gccagctacg gtcatctgat
    11461 gggtgaaatg ccgcgtgaag tgcgcgattt gctgtgcgat ccgcaaactt ctggcggttt
    11521 gctgctggcg gtcatgccgg aagcagaaaa tgaggtcaaa gctacagccg ccgagtttgg
    11581 cattgaactg acggcaattg gcgaactggt gccagcgcgc ggcggtcgtg ccatggttga
    11641 gattcgttaa ttcaatgcgg ttgtttattg ccgaaaaacc gagtctggcg cgcgccattg
    11701 ctgatgtcct gcccaaaccg caccggaaag gcgatggctt tatcgagtgc ggtaatggtc
    11761 aggtggtgac ctggtgtatc ggtcacctgc ttgagcaggc gcagccagac gcctacgaca
    11821 gccgctatgc gcgctggaat cttgcggatt tgccgattgt cccggaaaag tggcaattac
    11881 agccccgacc ctccgtgacc aaacaactta acgtcatcaa acgcttcctg catgaagcca
    11941 gcgaaatcgt tcacgccggg gacccggatc gtgaagggca actgctggtg gatgaagtgc
    12001 tggactatct gcaactggca ccggaaaagc gccagcaggt gcagcgttgc ttgataaacg
    12061 acctgaaccc gcaggcggtt gagcgggcgc tcgaccgtct tcggtccaac agtgagtttg
    12121 tgccattgtg cgtttctgcg ctggcgcgag cgcgtgccga ctggctgtac ggcatcaata
    12181 tgacccgtgc gtataccatt ctcggtcgca atgccggtta tcagggcgta ctttccgtgg
    12241 gacgcgtgca gacacccgtg ctcgggctgg tggtgcgccg cgatgaagag attgaaaact
    12301 tcgtggcgaa agacttcttt gaagtcaaag cacatatcgt gacacctgcc gatgagcggt
    12361 ttaccgctat ctggcaaccg agcgaagcgt gtgaaccgta ccaggatgaa gaagggcgct
    12421 tgttacatcg tccactggcg gagcatgtgg ttaaccgcat tagtggtcaa ccagcaattg
    12481 tcaccagcta taacgataaa cgggaatcag aatccgcgcc gctgcctttt tcgctttcgg
    12541 cgttgcagat tgaagcggca aaacgctttg gtctgagcgc gcagaacgtg cttgatatct
    12601 gccagaagct gtacgaaacg cacaagctaa tcacttatcc gcgttccgat tgtcgctatt
    12661 tgccagaaga acattttgcc ggacgccacg cggtgatgaa tgccatcagc gttcatgcac
    12721 cagatctgtt gctgcagcca gtggtagatc cagatatacg caaccgctgt tgggatgaca
    12781 aaaaggtcga tgcacaccac gccatcatcc cgaccgcacg gagttcagcc atcaacctga
    12841 cggagaacga agcgaaggtc tataacctga ttgcccgtca gtacttgatg cagttctgcc
    12901 cggatgtggt gttccgcaag tgtgttatcg aactggacat tgccaaaggc aaatttgtcg
    12961 ctaaagcgcg ttttcttgct gaagcaggct ggcgcacgct gttaggcagc aaagagcgcg
    13021 atgaagaaaa cgacggtacg ccattgcctg tggtggcgaa aggcgatgag ttgctgtgtg
    13081 aaaaaggtga agtggtagag cggcaaaccc agccgccgcg ccattttacc gatgcaacac
    13141 tgctttcggc gatgaccggg atcgcgcgct ttgtgcagga caaagatctg aaaaagatcc
    13201 ttcgtgcgac cgatggtctg gggacagaag caacccgtgc cgggattatt gaactgctgt
    13261 tcaagcgtgg ttttctgacc aaaaaagggc gctatatcca ctctaccgac gccggaaaag
    13321 cgttattcca ttcgctgcca gaaatggcga cgcgaccgga catgaccgcg cactgggaat
    13381 cggtgctgac gcaaatcagc gaaaagcagt gtcgctatca ggactttatg cagccgctgg
    13441 tggggacgct atatcagctt attgatcaag ccaaacgtac gccggtgcgg cagtttcgcg
    13501 gcattgtggc tccgggcagt ggtggcagtg ctgataagaa aaaggctgca ccgcgtaaac
    13561 gtagtgcgaa aaaaagtccg ccagcagatg aagtcggaag cggggcgata gcgtaagcga
    13621 gtgaatcttt cgtgctattc gag
//
LOCUS       DAZRUF010000104        13511 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZRUF010000104 DAZRUF010000000
VERSION     DAZRUF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.104"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(214..525)
                     /locus_tag="ACHXOL_002408"
                     /pseudo
     CDS             complement(214..525)
                     /locus_tag="ACHXOL_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            complement(544..2421)
                     /gene="bglF"
                     /locus_tag="ACHXOL_002409"
     CDS             complement(544..2421)
                     /gene="bglF"
                     /locus_tag="ACHXOL_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR6809370.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPTVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2555..3391)
                     /gene="bglG"
                     /locus_tag="ACHXOL_002410"
     CDS             complement(2555..3391)
                     /gene="bglG"
                     /locus_tag="ACHXOL_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR6809371.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3677..4402)
                     /gene="phoU"
                     /locus_tag="ACHXOL_002411"
     CDS             complement(3677..4402)
                     /gene="phoU"
                     /locus_tag="ACHXOL_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR6809372.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4417..5190)
                     /gene="pstB"
                     /locus_tag="ACHXOL_002412"
     CDS             complement(4417..5190)
                     /gene="pstB"
                     /locus_tag="ACHXOL_002412"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR6809373.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5281..6171)
                     /gene="pstA"
                     /locus_tag="ACHXOL_002413"
     CDS             complement(5281..6171)
                     /gene="pstA"
                     /locus_tag="ACHXOL_002413"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR6809374.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6171..7130)
                     /gene="pstC"
                     /locus_tag="ACHXOL_002414"
     CDS             complement(6171..7130)
                     /gene="pstC"
                     /locus_tag="ACHXOL_002414"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR6809375.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7217..8257)
                     /gene="pstS"
                     /locus_tag="ACHXOL_002415"
     CDS             complement(7217..8257)
                     /gene="pstS"
                     /locus_tag="ACHXOL_002415"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR6809376.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8505..9578)
                     /gene="lpfD-O113"
                     /locus_tag="ACHXOL_002416"
     CDS             complement(8505..9578)
                     /gene="lpfD-O113"
                     /locus_tag="ACHXOL_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR6809377.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9589..12111)
                     /gene="lpfC-O113"
                     /locus_tag="ACHXOL_002417"
     CDS             complement(9589..12111)
                     /gene="lpfC-O113"
                     /locus_tag="ACHXOL_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR6809378.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSCVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(12136..12867)
                     /gene="lpfB-O113"
                     /locus_tag="ACHXOL_002418"
     CDS             complement(12136..12867)
                     /gene="lpfB-O113"
                     /locus_tag="ACHXOL_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR6809379.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(12915..13487)
                     /gene="lpfA-O113"
                     /locus_tag="ACHXOL_002419"
     CDS             complement(12915..13487)
                     /gene="lpfA-O113"
                     /locus_tag="ACHXOL_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR6809380.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSLVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         3556 a         3441 c         3055 g         3459 t
ORIGIN      
        1 cacgttagcg cttggcgacg gcggaacaaa gctgattcgc gacgtaagaa agtggcaggc
       61 tcatatgacg gacctctgta atgcggtatc caacccgcga atatcagcgt gattcaccgt
      121 cggatttact gctacgtcgc cctcagggaa ttaaacctaa aaagaaaatc gtcaaaatta
      181 gagattccgg tttgacacgg aaagtcatat caacgctaac cccgcttcat tcggttcggc
      241 ttcgtcgccc tgagggaaaa ttcgcgccca ggcaatggaa atacgcagac aggtgaagcc
      301 catctcggca aataacgcga tatcttccgg gtaacggtga taaaaatcga tggcgacatc
      361 tttgatattc tctttcccca ggatgcgcgg ttccattttt cccataacgc catgaggctg
      421 taaatctgag gtcgtgctcc ctttgccatc ttccagccag gcaccttcca cctgattggc
      481 agctgttgcg ccaccccaaa gaaatgtttc tggaaatgct ttcataatta actcctttta
      541 tcgttagcga atgatggata acagcggttc acctgcgctt atctgcgccg tgccgtgggg
      601 taatacgtct gtaaaatcat cgctattact gattaatacc ggcgtcgtca catcaaatcc
      661 ggcctcgcga atagcaggga tatcaaaaga aatcagccga tcgcctgtat tgaccttgtc
      721 acccacgttg acgtgagcgg aaaagaattt gccgtccagt tttacggtgt cgataccgac
      781 atgaatcagg atctccacac catcatctga ctcaatgcca atggcgtgta atgtggcgaa
      841 caacgaagca attcgacccg caaccggaga acgcacttca ccaaccgtgg gcagaatggc
      901 gataccttta cccaacaggc cactggcaaa cgtggtatca gcgacgtgaa tgagcggcac
      961 aatctctccc gtcatcggtg aacagatacc gccctgctca ggtggtgtaa taacctctgg
     1021 tgttttctct tgcggggcac cctgcgctgg ctgacgttta gcggtgatga aatgaagcat
     1081 caccgtaccg acaaatgcgc aaccgatggc aatgacaccg ccaataacgc tggcccagac
     1141 ggtgaaatca attcccgttg acgggatggt ttgcatgaag gtgaaaatac ttggcaaacc
     1201 aaaggagtac actttcgttt gcgcgtagcc aatgatggtg gcccccaaag ccccactgat
     1261 acaggcgata acaaaggggt acttacgcgg cagattaacg ccatataccg ctggttcggt
     1321 gataccaaac aggcccgtca acgccgctga tcccgccacc acttttttct gcgcatcgcg
     1381 ttcgcagagg aagacgccga gcgccgcccc gacctgcgcc ataatggcgg gcattaacag
     1441 cgggatcatg gtgtcgtagc ccagcacggt gaagttattg atacacagcg gcaccaggcc
     1501 ccagtgcagt ccgaacatga cgaagatttg ccagaagccg cccattaccg cgcccgcaaa
     1561 tgcaggaacc gcctgataaa gccagagata accggcggca atcagttcgc ttatccaggt
     1621 tgatagcggc cccaccagca gaaaggtgac gggtgtgata accatcagac atagcaatgg
     1681 tgtgaagaaa tttttgattg ccgacggtaa ccacgcatta agtcggcgtt ccagaatgct
     1741 gcacaaccag gcagaaaaaa taatgggaat aaccgatgac gagtaattca acaatgtgac
     1801 cggaataccc aggaaatcca gccccagcgc atccgctttt tgcccgttct cgaaagcagt
     1861 cagaattaat ggatgcacta acgctccacc aatcaccatg gcagtaaatg gattaccgcc
     1921 gaagcgtttc cccgcggtgt atcccaggat tatcgggaag aaccaaaaca aggcatcact
     1981 ggcgctgaat aaaattaaat aagtaccact ttgttcggtc gtccactgaa aagtgagcgc
     2041 cagagccagc atacctttca ggatcccggt tgccgccatc aaaccgatca gaggcgtaaa
     2101 aatacctgaa ataacataaa caaagcggtt tagcagatta cctttctcat cattttccgg
     2161 tgcctgttgc gctttttcgc caaggcctgc cacgctgttg accgccagga agacatcggc
     2221 cacatggtta cctatgacca cctgaaactg gccaccgctt tccaccacca taataatacc
     2281 gggggtgttt ttcagtacct cagcttgcgc tttgctttca tcctttaact taaaacgtaa
     2341 tcgcgttgcg caatgcatca gactaacaat gttatctgcg cccccgactc ctgcgactat
     2401 ttttctggct aactccgtca taacttgccc tctaccgctt tgcggcaaaa ctcccaaaaa
     2461 aaaactgaaa aaaacggcct gacgtgaatc aagcaatttt tttcaggttt tgcccgctta
     2521 gtgcggtaac aatcctttac tcagtaataa tatttcagtg ttctttgcgc acgcgctcta
     2581 tatttatggc taaaaacata atctctgcgg gtgaaatttt acgttgatac tgcaaaccaa
     2641 taaaaatggc gatccgttcc gcacattgcc atgcttgcgg gtaattttgt tttactgctt
     2701 gttgtaatga ttcatcacta tcgttaattg aagcatgttc aagaatacgc caggataaaa
     2761 acttcagatg tgtaaccagt cgctgataac tcaagctttc ttcctggtaa ttaaggctga
     2821 actgaaattt tattaattgc agcatttcgc gcattaactg cgtgacacct gcaacatcct
     2881 ccatatttcc gctcatttgg gcactgacca gatgcatggc aataaagccc acttcatctt
     2941 tcggtaactg cacgcccaac cgtttatcaa taatggttaa cgcttcttcc cctagctgga
     3001 actctttcgg gtaaagccgc tggatatccc acagcaacgg gttgggcagc aacacgtttt
     3061 gctgaaagcg tttaatcgca aactggcaat ggtcagttag cgagatataa atactgtcct
     3121 gtaattttcc caagcgctcc tgcgctaaag agataatacg atcacaggtt gccatcacct
     3181 caagaggaat atgacttaag agttcgctta atcgcccgtt cagttcatga ctgctcaagg
     3241 catactcttt ttctattcca cttgagttaa ttctttcgcc agcgcatttt tgaaagccaa
     3301 ttcctcgccc catgacgact ttttcccgct gttgatcatc aataaccacc acaacattat
     3361 tgttgagaat tttggtgatt tgcatgttca tagcaaggac cttttttata aacaaaaaaa
     3421 accgacttca ccagtattct ctggttatgt caggttttgc ctgcgaatgc agtaacaatc
     3481 cagtcattta ttaatggttt ttataacgaa catccaggtt cggaaattaa tttaattgcg
     3541 tgcagagaaa atagcaatgc gctattgata aaaatatgac catgctcgca gttattaact
     3601 ttgtgtaatt ttaggaattt ataaagttat atataacaaa tcccaataat taagttattg
     3661 ggatttgtct ggtgaattat ttgtcgctat cttttcccgc cagcagttta tccagctcat
     3721 caccgcctac atgacggaaa tcctgcccct tcacgtagta gaagataaac tcacaaatat
     3781 tctggcagcg gtcgccaata cgttcgatag aacgcgcgca gaacagcgca gtaagtacgc
     3841 tcggaatggt acgcgaatct tccatcatgt aggtcatcag ttgacgaaca ataccttcgt
     3901 attcctgatc gactttttta tcttcacgat aaatacgtac cgcttcgtca atgtccatcc
     3961 gcgcgaacgc gtccagcacg tcgtgcagca tctggatggt atgacggccc agcgactcca
     4021 gacttaccag caacggctga tgctgctggg agaatttctc cagcgcagta cggcagattt
     4081 tgtccgccac gtcgccaata cgctccagct cggcaatggt tttactgatc accataacca
     4141 gtcgcaggtc gctcgccgtc ggctgacgtt tggcgataat gcgcacgcag gcttcatcga
     4201 tcgccacttc catcatgttg acgttcttgt cgccttcgat cacgcgcttc gccagatcgc
     4261 tgtcctggtt atgcatcgcg gtgattgcat cagaaagctg ctgctccacc atgccgccca
     4321 tggtcatcac ctgcgtgcgg atactttcca gttcggcgtt gaactggccg gaaatatgtt
     4381 tattaagatt gagactgtcc ataacgcact cctgaatcaa ccgtaacgac cggtgatgta
     4441 gtcttctgtt tgtttcttcg ctggcttggt gaacagatcg tccgtgttgc tgaactcaat
     4501 caattcgccc aggtacataa acgccgtgtg gtcggaacaa cgcgcagcct gctgcatgtt
     4561 gtgggtaacg atcaccacgg tgtaatcctg cttcagttcg gtgatcagct cttcaatacg
     4621 cccggtagag atggggtcga gcgccgaaca cggttcgtcg agcagcagca cttccgggcg
     4681 aatggcgata ccacgcgcaa tacacagacg ctgttgctga ccaccagaga gagagtaacc
     4741 gctctggtgc aatttatctt tggtttcgtt ccacaatgcg gctttggtca atgcccactg
     4801 cacgcgctcg tccatgtcgg cacgggagag cttctcaaac agacgaacac caaaagcgat
     4861 gttgtcgtag atggacatcg ggaacggcgt cggtttctgg aacaccatgc ccactttcgc
     4921 acgcagcagt gcgatatcct gagagttggt caggatgttg tcgccatcaa gcagaatttc
     4981 accttctgcg cgctgctccg gatacagttc aaacattttg ttgaaggtac gcagcagcgt
     5041 cgatttaccg cagccggacg gcccgataaa cgccgttacc tggtttttag cgatatccag
     5101 gttgatgttt ttcagggcat ggaatttgcc gtagtagaag ttcaaattac gaacctgaat
     5161 tttactcggg gcagtttcaa ccatactcat tgcaatcttt tcctcatcgt acctgatgct
     5221 tttcgctaat caggcttacc gtaggccggg taaggcgttc acgccgcatc cggcaaagtg
     5281 ttaaccgtgt ttattcttcg caaaaacaac gcgcgccaga atgttcagca gcaggacgca
     5341 cagggtaatg atcaataccc cggcccaggc cagttgttgc cattccgcaa acgggctcat
     5401 cgcaaactta aagatcgtca ccggcaggtt ggcgatcggc tgcatcatgt ccgtgctcca
     5461 gaactggttg gagagcgcgg taaacagcag cggcgcggtt tcaccggcaa tacgggcaat
     5521 cgccagcagg ataccggtca taatcccgga caccgacgct ttcagcgtaa tcgcggagat
     5581 catcttccac ttcggtgtac ccagcgcata agccgcttca cgcaggctgt ccggcaccag
     5641 tttcagcatg ttctcggtgg tgcggataac gatcggcacc tgcaacagcg ccagcgcaat
     5701 tacgcccgcc cagccggaga agtgctccat ctgtgccacc acgatggtgt agacaaacag
     5761 accaaccaca atcgacggcg cggagagcag aatgtcgtta atgaagcgaa tcacttctgc
     5821 cagccaggat ttacgaccat attccgccag ataaatcccc gccataatgc ccagcggcgt
     5881 accgaatacc gtggcccaca aaattaacag cccgctaccc gccagggcgt tcgccagacc
     5941 accaccttcc gtattgggcg gcggcgtcat ttcagtgaac agcgccagcg acataccgtc
     6001 gataccgcga gtgatggtgg acattaaaat ccagatcagc cagaacaggc cgaaggccat
     6061 cgtcgccatc gagagcgtca gcgcaatacg gtttttgagg cggcgacgcg cctgcatttt
     6121 gcggcgagat tcagccagcg ccgcagtggt ttgcatttca accatagcca ttagcgtgcc
     6181 ccctcattct tagccaggcg cataatcata aacttcgatg cggcgaggac gatgaaggta
     6241 atcacaaaca ggatcaggcc cagttccatc agtgcggcaa cgtgcagacc ggattccgct
     6301 tccgcaaatt cgttcgccaa cgcagaggtg atactattgc ccggcatgta cagcgaggcg
     6361 ctgtcgagct ggtaggtgtt accgatgata aaggtcaccg ccatggtttc accgagcgcg
     6421 cggcccagcc ccagcatgat gccgccgata acaccatttt tggtgaacgg aagaacgata
     6481 cgccagataa cttcccaggt ggtgcagcca ataccgtagg ccgactcttt catcatcacc
     6541 ggggtttgtt cgaacacatc acgcattacc gccgcaatgt acggaataat catgatggcg
     6601 aggatcacgc ctgccgcgag gataccgata ccaaatgcgg ggccagagaa cagcgcgcca
     6661 acaatcggga tattcgacat gatattgccg accggctcct gaaagtaaac ggcgaacagc
     6721 ggcgcaaaga taaacaggcc ccacatgccg taaacgatac ttggaatggc tgccagcagc
     6781 tcaatggcga tacccagcgg gcgtttcagc cagccaggcg caagctcagt caggaacagg
     6841 gcgataccga aactcaccgg gacggcgatc agcagcgcga taaacgaagt caccaacgta
     6901 ccgtagatcg gcaccagcgc cccgtagata tcgttcggtg catcccactc tttggtccat
     6961 aggaaagcca gaccaaattt ctgaatgctc ggccaggagg agatgatcag agagacaata
     7021 atgccaccca acatcaatag cacaatcagc gccgccagtt ttaccagcac gctgaaaatt
     7081 atgtcgccct ttttacccgg tgggttaaaa gcaggcttgg ttgcagccat aagttactct
     7141 tcagttaaac gcgtttacaa agtcattttg taatgccgga tgcggcgtaa aacaccgtac
     7201 ccggcctaga gttttattag tacagcggct taccgctact gtctttaata ttggtcttcc
     7261 acgcagcgcg aacctgttca actacactat ccggcaggct ggcgtaatcc aggtcgttcg
     7321 cctgtttagc cccggttttg tacgcccagt cgaagaattt cagcacttct gtgccttgtt
     7381 ctggtttctt ctgatctttg tggatcagaa tgaacgtggt agaggtaata ggccatgcat
     7441 cttcgccttt ctggttggtc agatcctgag cgaaggtttt gctccagtct gcaccttttg
     7501 ctgcattagc gaagttttct tcggtcggac taaccggttt accatcagcg gagatcagtt
     7561 tggtgtacgc caggttgttc tgcttcgcgt aagcatattc aacataacca attgcacccg
     7621 gcagacgctg aacgaacgcg gcgataccgt cgttaccttt accgcccaga ccgatcggcc
     7681 attttacggt agagccagta ccaacgttgt ttttccactc ttcgttcact ttcgccaggt
     7741 agctggtgaa gacgaaggaa gtcccggagc catctgcgcg gcgtactaca gcaatgtttt
     7801 gtgaaggcag tttcagaccc ggattcagtt tggcgatggt ttcatcatcc cacttcttga
     7861 ttttgcccag gtagatgtcg ccgagggttt taccatccag caccagttcg ccagacttca
     7921 gccctggaat gttaaccgcc agcaccacgc cgccaatcac ggtcgggaac tggaacagac
     7981 cttcctgagc cagtttttcg tcagacagcg gcgcgtcaga ggcaccaaaa tcaacggtat
     8041 tagcgataat ctgttttacg ccaccggaag aaccgatacc ctggtagtta actttattac
     8101 cgatttcttt ctggtaagtg tcagcccatt tggcatacac cggcgcaggg aaggttgcac
     8161 ctgcacctgt caggcttgct tctgcaaaca cagagaaagc gctcatcgat aaggtcgcgg
     8221 cgacaacagt tgcgacggtg gtacgcataa ctttcataat gtctcctggg aggattcata
     8281 aagcattgtt tgttggctac gagaagcaaa ataggacaaa caggtgacag ttatatgtaa
     8341 ggaatatgac atttttatga tagaaaggaa ttaacaaata acttactgat tttaaatgtg
     8401 aagatgaaaa tcccacatgt catctttttg tcatgatgca tttaaaagtg ataaagaaat
     8461 gggtgagatg acaacgatgt gatgaacaat cgttgtcaga cctattagcg cacaatctca
     8521 acagttatcg tcgctgttgc agtaaattgc cccggggctg ggcgagctcc agtaaggctc
     8581 gcaggagcag ctgaaaaagt tacactacct gatttttcac catacactgt tgatttaccc
     8641 atgtctgttg gcaactcacc accgttcact ttgatttcct tattattttt gtcgtaaatt
     8701 tttatcccca cgttagagtt tgaagttacg accattgaat tgtccgtatt gggttcggta
     8761 gcataaaaag acgtactgat atcatcggta ttcagtatcc ccaggttttc acactcaata
     8821 gtgactgttt tagtaatttt atgtgttgtt accgcacttc caacaacaga tgaaaaatct
     8881 gcagctggta tttctttaaa atcaaaataa actggctgta aattatttat ttcgcaactt
     8941 tgcggagctg taatatcgcc ccgaatataa acatcagcca tcggctgtga gtaatccacc
     9001 ggggtgtcac ggctaattgt tccatacaat gtcactatct ttgtttcagg aataatcaat
     9061 tttcctgcaa atgtcttctt gatataaaaa tatactatcg ctttaccccc actggatata
     9121 cttaaaggtt caatgcggtt gcaattgtac tttgtattac cagacgggtt gttagcatga
     9181 tattcaaaag gtgcattcac atacccaacc ccaagaatat ctatagcaat cccaacatca
     9241 aggttcggat taagatcata ataattaata ttagagtgaa ccccagcata agttaatgcc
     9301 tggtttattt ttgacgtata atagatatag gggaattctc ctacattacc agtatcacaa
     9361 tgacagcgtc caggatatga ggatccatta gttacagtgg tattaaatgt cgttccaacc
     9421 ttattttcgt tcgcggtaat aagatatccc gagaaatcaa gcgtacggtg aaatacgcca
     9481 ttatcagggg tacagatacc tttaggagct gcaagtgcta cactacttaa cgagagcata
     9541 aacacagcaa agcaccactg tttgatatac ttgttcataa aaatcctttt aatgacatgt
     9601 cacaccctgt tcaattaatc cgctctcact ttctggtgtc gaaagtgaat aattgaagat
     9661 gcattttgtt gaggcgtttt ctccccagga taacgtaacc tgtccttttt ctggtagacc
     9721 agaaagataa agttcgccac ggtcaccgac aattcctgaa tctgccaaac ttgcatcttg
     9781 tatcgcagca atggctccaa aaggtaccgg tttaccattt gcatttaaca ctctgaagag
     9841 aacgcgataa cccacatgag tcacaaactc cgctttaacc actgcaccac gcgtgggaac
     9901 catcgtaacg acggtattat caagttctat attcgacttc gagaaatagt tatcgctgat
     9961 tgctacccga ttttcatcat aagggtttag ctgtgtcttg accgtatagc cgcgccagtc
    10021 cgtcgcagca ccgcgcatat tatctatttc taacccggcg gccccaggtg ctttaacaag
    10081 agcaatagtt tcacctaact cctgacttaa cgttacccct tcgctgtgtg ccagaattcc
    10141 ccctctgata ccataattca gcctctggga attttggctg tagttatagc tgccgttcac
    10201 cgtgccataa gagccatcat aggtcaggga catattaccg ccaacctcat catcatgact
    10261 gttataggac tgcattaaat tccatgaaag gttatttctt tccagcgccg taccgctgag
    10321 tccgagttga tgtgagctac cggaattatc gttttgcgaa acagaatagg tggcgtaact
    10381 attaccccac cagcccaata aaggaacaga taaagagagc atgtagatct gctcactggt
    10441 gttatcactg gcatggttgt tccccgtccc ctgtctggca gtattggaat aactccagga
    10501 gacgttccac gagagacgct tccagctact gctgtagcca aattgcatta atcgttcgac
    10561 atcatcggta tgccagtatt gttgccggct taatgtcagg tataaattgc cataatctct
    10621 ccccaacgac tgcgttagcg tcccctccac gcaactacgt cggttacctg tttcccaaaa
    10681 gtcttcaggg ctgttgcgcc gcgatgccca ctcattgagg gtatagtagc cctcggtgga
    10741 gtaacgatag ccaaccaagc gcaagctagt gtctgtgtcg tcaaatcgtt ttgaatagag
    10801 aaagcgatat gactgacccg tttctgtatc atcatggcta aaattcgcac gagcatgtgt
    10861 cacatcgaaa gaaattgcgc ccaaagcgcc aagatcttta ccgacaccca gagctgcaga
    10921 accataattt tcagcagcct gcatacctcc atataaagtg atcccgtggg aaaaaccatg
    10981 aagtatttgc gcctgaagga cgtccggtgt aaatccgtct tcatcgcgca attcacccac
    11041 gctgacatca acatctgtct gaccttcacg tttaagaatc gctaatgaag cataaggttg
    11101 ggtgaatcgt tgttcgctac cgtcttcttc tttgatggtt acgtccaaat cgccacctgt
    11161 actggcggtg ttgagatcgg tgatctcaaa agcaccttgt ggaacggcag actgataaat
    11221 cacgtagcca ttctgccgaa tgatgacggt ggcgttggac tttgcaatcc cacgaaccac
    11281 aggagcaaag ccattctggc tggcgggtag catatcgtta tcagtataca aacgcgcgcc
    11341 gcgaatttgc gtactgtcga aaatatcgct cgccgtccac gtatcaccaa tcataatctg
    11401 gcttcttaac gcggcaatat cgcgctgtat ccaggtacta atggagttgt attccgcacg
    11461 gccatcgctg gtgtttaaag tactgtaatt acgtagccgc cacggtccaa tattcagtcc
    11521 attgcgcaga ttgagataac tggtatctgt ctcttgtgaa ccataatctg aacgtagtgt
    11581 atgtgaacca gaaaaatcgt agttgatggt gagcgcgtta atcccctcat cccaacgcgt
    11641 ggggctgatg taatcataag ccatcgcgtc catgtagatt tggggaatgg atatattcag
    11701 ctgcaattta tcactgtcaa actgccagga tgagccttca atacgtggag caggatcgag
    11761 gcaaaccgtc tcatccacat cattgaacga tttgagcgca tcaactttta tgccaaagga
    11821 ggacagtgtt tttttaatgg caataaagca agcgtgcaga tcagcatctg gattatcctg
    11881 aacaaatgtc acatcctgac gttcaaacat tttaccgttg ataaaaatat caacgctata
    11941 aacacctggg ggcatcaggt ttgcccgatt aaactggcga atatcgactc cggattgatg
    12001 gtcgatttcc agaagcgccg gattgaatac actgacctcg gccatcaggt tttgcgagaa
    12061 aatcagacac gtccctgccg tgaggccaac cactattctg gtcgtcatca taatacgcct
    12121 atccttatcc cttgcttaca gcgtcatttt tatcgggtca ctgactgcgc cgtaatcatt
    12181 aataaatcgc caggtcattt ggcctcccgc agaattagca ggtaaaggta acgtgagcgt
    12241 ttcaaaagga cggatcatat tgaggggcac atcatggcca tcaacgatga catccatcac
    12301 actgatatga attgggttgg gattctctgc tatcagattt cgcccttccc ggcgccattt
    12361 cactttcggc gcagcatctt cgggaataag gcggatattt gctggtcgcc agaacagctt
    12421 gatacgcgtt tttactgcta tttccagcga gttcgtcgct ttgttgctcg ctgatggcgg
    12481 aatattttta atattcagcc agaagactga ctcgcgatca tcaggcaatg acacaccttt
    12541 ggtgtgaata atgcgtaata ttttttcttg cgaaggttct aatcgtgata ctggcggaat
    12601 aacggtaaac ggcgctttgc ttttatcctc tttattaaat ggatctaccc aagcctggat
    12661 gagataaggt attttttctt ctttgctaaa tacagaaata gatttatcag cattattact
    12721 taagtaaacc acccgcgtac caccaatgac aacgcctgca taagttaatg ctggttggcc
    12781 taaaaaaacg ctgagtataa tcgcggtaaa taacttacat gaaactgttg ttttaaagag
    12841 cctgattaaa caactttttc tggacataac gactccctgg ccaaagccaa ctgaataaaa
    12901 aggcccttga taaattattt cttatattcg acagtaaatt gtgaatcagc gttcgccgta
    12961 ccagccgtta cggtcgcatt ggttgccaca tagcgtgcaa taaacttcat ttcagccttg
    13021 ccttcagcat cgatagcggt ataagcagag tcgttataaa gtttaaccag cgaattatcg
    13081 gactggttaa aaatacgaat accaacgccg gttgccgcaa tcgcttgtcc tgtaccggta
    13141 aaatcaccag ggttgcctgc tgtaagcggc gtacccactt tcaggtcgcc gtcgccgcca
    13201 ggcgcttctg taccatcaaa acgaacggct gcttgagtgt acgttgccgg gcaattttca
    13261 agtttaatac tgaacttctg tggcgacgcc agactaccta cacctgaaaa tgcattttta
    13321 ctgactttac ccagtgggac aatttgatcg gctgtatcgg aggtaacttc acaagtagtg
    13381 tcgacaattt caccgacgaa gtgaacaaca ccatcttctg ccagtgcatg cccggccagc
    13441 attagagatg ccagagtgaa tgcaccgcct ataatattac gcttcataaa aactccattg
    13501 atataatata t
//
LOCUS       DAZRUF010000105        13423 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZRUF010000105 DAZRUF010000000
VERSION     DAZRUF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13423
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.105"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..412)
                     /locus_tag="ACHXOL_002420"
     CDS             complement(<1..412)
                     /locus_tag="ACHXOL_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR6809381.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVK"
     gene            513..1112
                     /locus_tag="ACHXOL_002421"
     CDS             513..1112
                     /locus_tag="ACHXOL_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR6809382.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            1220..1987
                     /gene="tpiA"
                     /locus_tag="ACHXOL_002422"
     CDS             1220..1987
                     /gene="tpiA"
                     /locus_tag="ACHXOL_002422"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR6809383.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(2042..2797)
                     /gene="cdh"
                     /locus_tag="ACHXOL_002423"
     CDS             complement(2042..2797)
                     /gene="cdh"
                     /locus_tag="ACHXOL_002423"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR6809384.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(2904..3893)
                     /gene="sbp"
                     /locus_tag="ACHXOL_002424"
     CDS             complement(2904..3893)
                     /gene="sbp"
                     /locus_tag="ACHXOL_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR6809385.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(4213..5175)
                     /gene="pfkA"
                     /locus_tag="ACHXOL_002425"
     CDS             complement(4213..5175)
                     /gene="pfkA"
                     /locus_tag="ACHXOL_002425"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR6809386.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(5356..6258)
                     /gene="fieF"
                     /locus_tag="ACHXOL_002426"
     CDS             complement(5356..6258)
                     /gene="fieF"
                     /locus_tag="ACHXOL_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR6809387.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(6493..6711)
                     /gene="ogrK"
                     /locus_tag="ACHXOL_002427"
     CDS             complement(6493..6711)
                     /gene="ogrK"
                     /locus_tag="ACHXOL_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFR6809388.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(6793..7956)
                     /locus_tag="ACHXOL_002428"
     CDS             complement(6793..7956)
                     /locus_tag="ACHXOL_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR6809389.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDITLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHCLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(7956..8435)
                     /locus_tag="ACHXOL_002429"
     CDS             complement(7956..8435)
                     /locus_tag="ACHXOL_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6809390.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(8450..10897)
                     /locus_tag="ACHXOL_002430"
     CDS             complement(8450..10897)
                     /locus_tag="ACHXOL_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR6809391.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRRASKLLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTIAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWVQKNKSLASTIGLIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(10890..11045)
                     /locus_tag="ACHXOL_002431"
     CDS             complement(10890..11045)
                     /locus_tag="ACHXOL_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR6809392.1"
                     /translation="MTLPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(11042..11317)
                     /locus_tag="ACHXOL_002432"
     CDS             complement(11042..11317)
                     /locus_tag="ACHXOL_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR6809393.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(11374..11892)
                     /locus_tag="ACHXOL_002433"
     CDS             complement(11374..11892)
                     /locus_tag="ACHXOL_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR6809394.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(11905..13095)
                     /locus_tag="ACHXOL_002434"
     CDS             complement(11905..13095)
                     /locus_tag="ACHXOL_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFR6809395.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTVSEVKAYRQNFSQRELMVIWPDFLAWDTVASTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
BASE COUNT         3119 a         3773 c         3297 g         3234 t
ORIGIN      
        1 acttcacatc ctgagtatct ttaccatatt tattttcgcc ttgtgtgcca acaaacgcac
       61 caagatcgat aagcatcatc accagaatca acgtcgggac aaaacgcccc accgcccatt
      121 gccagacacc cggtaaaatc gcccagttac ccgccagcag catccacgcc acaatcatca
      181 gaaatgccca tgcgccggaa cgcccgcgat catgcaagcg cttaacagtt actgccgctg
      241 ttggccaaag caagcacaca aggcaaaacg ccgcggtctg aatatcgagt aaatttttac
      301 ccgccagtga gaaaagcacc agcatgcctg cgaaccacag gcctatccaa atccagaaat
      361 cacggcgtcc aatacgccct ttaaatgaga ataaccattg ctgtatggtc atgtaagttc
      421 cttgatggtt gtcttttcca ggattctacc cttttgacaa gggcgacaga tatcgtttta
      481 atcggagcca gtcataacaa aaggtactgt caatgaagcc agggtgtacg ctgttttttc
      541 tcttatgttc tgcattaacc gttacaacaa cggcgcatgc acaaacacca gatacggcaa
      601 cgaccgcgcc ttatctgctg gctggagccc ctactttcga tctctccatc agccagtttc
      661 gagaagactt taacagccag aatcctagcc tgccactgaa cgaatttcgt gccatcgaca
      721 gcagtcccga caaagccaat ctcactcgtg ctgcaagtaa aattaacgag aacttgtatg
      781 cttctacagc gctggagcgc ggtaccttaa aaatcaaaag cattcaaatg acctggctac
      841 ccatccaggg gccagagcaa aaagcagcga aagcgaaagc tcaggaatac atggcagcag
      901 tgatccgcac actcacccca ttaatgacca aaacacaaag ccagaaaaaa ctgcagtcgc
      961 tactaacggc ggggaaaaac aaacgttatt acaccgagac agaaggtgca ctgcgttatg
     1021 ttgtcgcgga caacggcgaa aaggggctga ccttcgctgt tgaaccgatt aagctggcgc
     1081 tatctgaatc gcttgaaggt ttgaataaat gacaaaaagc aaagcctttg tgccgatgaa
     1141 tctctatact gtttcacaga cctgctgccc tgcggggcgg ccatcttcct ttattcgctt
     1201 ataagcgtgg agaattaaaa tgcgacatcc tttagtgatg ggtaactgga aactgaacgg
     1261 cagccgccac atggttcacg agctggtttc taacctgcgt aaagagctgg caggtgttgc
     1321 tggctgtgcg gttgcaatcg caccaccgga aatgtacatc gatatggcga agcgcgaagc
     1381 tgaaggcagc cacatcatgc tgggtgcgca aaacgtggac ctgaacctgt ccggcgcatt
     1441 caccggtgaa acttctgccg ctatgctgaa agacatcggc gctcagtaca tcatcatcgg
     1501 ccactctgaa cgtcgtactt accacaaaga gtctgacgaa ctgatcgcga aaaaattcgc
     1561 ggtgctgaaa gagcagggcc tgactccggt tctgtgcatc ggtgaaaccg aagctgaaaa
     1621 cgaagcgggc aaaactgaag aagtttgcgc acgtcagatc gacgcggtac tgaaaactca
     1681 gggtgctgcg gcattcgaag gtgcggttat cgcttacgaa cctgtatggg caatcggtac
     1741 tggcaaatct gcaactccgg ctcaggcaca ggctgttcac aaattcatcc gtgaccacat
     1801 cgctaaagtt gacgctaaca tcgctgaaca agtgatcatt cagtacggcg gctctgtaaa
     1861 cgcgtctaac gctgcagaac tgtttgctca gccagatatc gacggcgcgc tggttggcgg
     1921 tgcttctctg aaagctgacg ctttcgcagt gatcgttaaa gctgcagaag cggctaaaca
     1981 ggcttaagtc tgacaggtgc cggatttcat atccggcact tactttcctt aactcttcgc
     2041 cttaacgcaa aatctcacac tgatgatcct gaatttcctc ggctgaagca cggttaagcg
     2101 tcagtagatt tcgttgtgtc gccagcaata caaatgagtt atcactctgc cgtaccatcg
     2161 ccagcccgta gcttcccata tgttcccgcg cctcaggtac ttcttctgcc agcatcataa
     2221 atgggctgcg ttgtaccagt tcgctttccg ttacccgacg cgccaggtat tcatgcccgc
     2281 gcaaaccacc tggcagtggc aaccagcggc tgctgatgtt cgccagattg ttatccagtt
     2341 gttcgcgcac atcaggacga atacaagaga tatgaatatg aaaatggttt tgcgtacgcc
     2401 cggtgcggga gttgatcgcc aaagaaaccg cgcgatcggg aaccggctgg ccgtattttt
     2461 tgctcataaa atcacgcgcc tgccaggcca accaaaagaa gttcggcgtt gaaggatcgg
     2521 tcaacaaagg actttcagta ccgttaatac gatacgttgg catcaacaga tattgcagtg
     2581 gaccattaag atcttttaaa accacgtatc cggcattggg tttgacttcc gcacatggcg
     2641 aaggattttg attttgctgc tgattgggca aacattcctc aaggacaatc ttacgtaatg
     2701 tatccgactc ttcaccggtt aatttccagt aaccaatacc ggcagccaca acggcgataa
     2761 ctatcatcac caaaaaaaga agacccgctt ttttcatctt tttttccctg tacctcaaag
     2821 agatgcaagg gtaacgcaaa atcgtgacaa ataaaaaacc cggtcgcgag aaaccaccgg
     2881 gtttgataat tatcctgggg aaatcagcgt ttgctgatct gatcgaacgt accgccgtta
     2941 gcaaaatgct ctttttgcgc tttcgtccag ccgccgaact cttcatcaat ggtgaataac
     3001 ttcagctttg gaaacgcatt ttcgtacttt ttcgctactt cagcgtcgcg cggacggtag
     3061 tagtttttcg cggcaatttc ctgaccttct ggcgagtaga gatatttcag gtaggcttcc
     3121 gccacctctt tggtgccttt tttctcgacc actttatcga ccaccgacac ggttggctct
     3181 gcgaggatag actcactcgg cgtgacgatt tcgaatttat ctttccccag ttcattcgct
     3241 gccagcagag cttcgttttc ccaggcgatc agtacatcgc caatcccgcg ctcgacaaag
     3301 gtgttagtgg agccgcgcgc gccagaatcc agaacttcga cgtttttata cagtgcccga
     3361 acaaaatcct gtgcttttgc ctgatcgttg ttgttgtgat gcagcgcgta gccccaggca
     3421 gccaggtagt tccagcgtgc gccaccagag cttttcggat taggcgtgat caccgaaaca
     3481 cccggtttaa tcagatcgtt ccagtcatgg atctgcttcg gattgccctt acgcaccagg
     3541 aaaacaatgg tggaagtgta cggtgcggag ttatccggca gacgtttgat ccactcttta
     3601 tcaatccgcc cgcgttccgc aatcgcgtcc acgtcatagg ccagagccag cgtgacaaca
     3661 tcagcttcaa taccgttgat taccgacgtc gcttgtttac ctgaaccacc gtgtgactga
     3721 cgaatcacca cgttatcacc agtttgctgt ttccagtggg cgctgaatgc cttgttgtac
     3781 tgttcgtaca attcgcgcgt tggatcatat gaaacgttaa gaagctgaat atcctttgcc
     3841 ataacgctgg ttgccgccag caaaaatgtt aaccctacgc cccacttgtt catcgcccga
     3901 ctctcttatg ttgtgttgtg atgagcaaag cgtgccagaa ggttaaccaa acattaaaga
     3961 ataaaaaaag attggctata acttgcgggt atatgttgag ggattaaaaa ggcggaagaa
     4021 atccgcctca tattgctgac aaagtgcgct ttgtccatgc cggatgcggc gcgaacgcct
     4081 tatccggcct acaaaagttt gcaaattcaa taaattgcag aattcatgta ggcctgataa
     4141 gcgaagcgca tcaggcattt ttgcttctgt catcggtttc aggctaaagg aatctgcctt
     4201 tttccgaaat cattaataca gttttttcgc gcagtccagc cagtcacctt tgaacggacg
     4261 cttcatgttt tcgatagcgt cgatgatgtc gtggtgaacc agctgttcgt tctggatacc
     4321 tacacaacga ccgccgtaac ctgccagcag cagatcgata gcgtaagcgc ccatacggga
     4381 agccagaata cggtcgtaag gcaccggaga accaccgcgc tggatgtggc ccagcacagt
     4441 tgcgcgggtt tcacgaccgg tttctttctc gatgaaatgc gccagttcgt caacatcaca
     4501 catatgttcg gtaatcgcca cgatcgcgtg ttttttacct ttcgcgatac ccgctttgat
     4561 ttcgtttacc aggtcttcac ggctgaattc aacttccgga accacaacga attcacagcc
     4621 accggcaatg gccgcagcca acgtcagatc gccacaataa cggcccatca cttccaccac
     4681 ggaaatacgc tggtgagaag aagaggtgtc acgcagacgg tcgatcgctt ctacaacggt
     4741 gctcagcgca gtgaagaaac cgatagtgta gtcagtgcct ttgatgtcgt tgtcgatagt
     4801 gcccggcaga ccgatgcacg ggaagcccat ttcggtcaga cgcattgcac ccatgtagga
     4861 accgtcaccg ccgataacca ccagcgcgtc gataccacgt tttttcaggt tttcgatagc
     4921 cacggcgcgg atgttctcgt cgcggaattc cgggaagcgc gcagaaccga ggaacgtacc
     4981 gccacggttg atcatgtcag aaacgctgta acggtctagc tgtaccatac ggtcttcata
     5041 cagacccaga tagccgtcat aaatgcccat tacttccaga ccttctgtca gcgcagaacg
     5101 aacaaccccg cgaattgcgg cgttcatgcc tggcgcatca ccgccgcttg tcaacacacc
     5161 gattttctta atcatgacta cctctgaact ttggaatgca aaataaaatc tgttgccgga
     5221 agtcttcttg cacatcgaag tgatccaacg aatgtgcaaa tagtataaca atcacttcct
     5281 gctgaattga ttcaggtcag gccaaatggc ggtattttat acacaaaatg cgggtctggc
     5341 tctcttttat actgattatg aaagcataga ccgtttaccc tccctgggta cgacggaaca
     5401 ggggtcctga tggataatta catccgatcc cggaaaacgc cgtaaaatag cctgctctac
     5461 ctgatccgcc accatatgtg cctgaaccaa aggcagagag tcttccattt ccaaatgaat
     5521 ctgaataaag cgggtcggcc ctgactgccg cgtgcgaaga tcgtgagcgc cgctaacacc
     5581 cggccaggaa gtcacgatat caataatttc ttgccgttcc tcatcaggca atgcgcgatc
     5641 cagtaatgac tgtaccgcct catatcccat gcgtaacgcg ctatataaaa tatagatgcc
     5701 gattcccaat gcaaacagag catcggcgcg atgccagccg taccaggaca accccagcgc
     5761 cagcagaatt gcgccgttca tcataacatc agactggtaa tgtagcatat cagcccgcac
     5821 cgcctggctt tgcgtccggc gcaccaccca ccgctgaaac gagacaagga taatcgtaca
     5881 aattagcgcc acaattgtca cgataacccc gacgcctgga tctgtcatcg gtgttggaga
     5941 taccagatgt tgaatacccg tcaaaaacag gaatagtgcc gaaccggaga taaacatact
     6001 ttgcgccagc gccgcgaggg actcagcttt accgtgacca aacgagtgat tatcgtcggc
     6061 aggttgcagg gaatatcgca ccaccagtaa attcgtcaac gacgcgccga tatccaccag
     6121 cgaatccacc agcgcggcga gaatactcac cgacccggta taccaccatg caaaaatttt
     6181 aatcagcaat agcagcgaag ccatcgccgt cgcagcaatc gccgcccgac tgaccagccg
     6241 tccataagat tgattcataa atactcccgc tatcaactga cgctagtata acggaagcaa
     6301 atcatctgca atgcattaag cagcaggcaa attgaggata aaaaaacccc cacatcatgt
     6361 gggggaagac agggatggtg tcacaaaaaa gtacctaacc tgatgatata aatggattta
     6421 tgtcaaccgc tgtccacata gcgtccacat cgaccataaa taagcccctc aactgagggg
     6481 ctattttttt gatcacatcc acataatttg ctgccctgac ggcaacgggt gcggccttac
     6541 ggcgtggact tctcccggct tcacgatgta tcgctgtacc gactcataag tgatgaacgt
     6601 ggcgctgcaa ttcacgttct ggcactggtg ataacgctct tttgtcgtgt cagtgatata
     6661 gcgacttgta cgcgcatgtg cggcatgctg gcataaagga caatgaaaca tcgcgagcac
     6721 ctcttccggt tttgttgatg gtgccatttt agttaattta tccttataaa acaaacagat
     6781 aaataaacca cgtcactcat catcttctgt ttcgtactcc acatcagaaa gccttacctc
     6841 aagctccaag gacgtcgtga agccgctatt attgagacaa tgtgtcacct tagtgatggt
     6901 ccagtcctgc tcgtctatga cgcgcttaaa gcctgacact ttaaccggtg tttccgtgta
     6961 aatatctgca cgaccggtag ccaggctgat ggagaactcc gctacacccc gttgcagttt
     7021 atcccacttc gcctgagcgg cgcgcatggc ctgcgctttt gtggcatata ccgtggtcag
     7081 ggcaaaaacg ttgtcagctt caccggccat gtattcacct tcgcgcgctt ccggtacttt
     7141 tggcgctttc ttctgcgtga ccggtttcgc tttcgggtgc tccagtgcgc gcaggtgttt
     7201 ttctttcttt ttgcgtttca gttttacctt ctgcttttgc ggcttcgggt ctttggtgtg
     7261 taaccacttt gccgttacgc cggtgtaagc tccacggtca gcaatcgcaa aatgatgacg
     7321 gtcgccgtcg ctgcgggtga tggtaatctg cgggattttt ttaccgctgg ccgtcacccc
     7381 ctgccccgct ttgagaaaca gcagttttcc catttttacc gacacctcgc cgccgttgcg
     7441 ttcagcaaga cgggtcagga atttcgcatc agactcctgc gactggtcga tgtgtggaat
     7501 ttttattccg gccagtgacg gagcgacact ggcttccagc ctgttacggg aggctatcgc
     7561 ctcaacaatc gcaccgagcg taatgtcatg ccatgagcct tcacggcggg aattgagcgt
     7621 cccgcgaaaa tctgcactcc gggcgcggat ggtgaccaca tccggcgcgc cccggtgttc
     7681 aacctcatca acggtaaatt tccctttgca taccagggca aaacctttcc agccgatata
     7741 caccgtcagg acagcgccac gaaccggcag cccgacctgc ccgtcggcat cgttcagttc
     7801 aatatcaagc tggtcagcct caaagccccg gttatccgtc agggtcatgc tcatcagacg
     7861 gtcgctgata ttgccggtaa tatccctgct gtcgagcatc agcatgtaat ccggcgtcag
     7921 cgtactgcct gcatcaaatg tcagcgcatc cagcattatc ccgcccccgt catacccgtg
     7981 aatttagtcg ccatactgcc agccttaccg atgagcgatt ccgcctgttt accgatatcg
     8041 ccataaagcg cggccagtga ctcatcaacg cgggtgagcg acagcgtaaa atcaattttc
     8101 cgtggtgtgc cgtctgcaaa gaaaatactc cctgtttcac tcaccttgct gatgacatac
     8161 atgccgtaaa tcatgccggt gccatccagc aacggccacg cccggccttc ctctgccatc
     8221 agtctgagcg tggtcatcgt cagctttccg ccagtcagtt cgggataaag cacaccggca
     8281 agtgtgatgt tctcctcacc tacaccgaga aactgatagg catcccgttt accgatacgg
     8341 gaatttgacg gccagcgata atctgattca cgctgcatgg tctggtgtgg cagcgtctgg
     8401 cgcataaaaa caaacatacc taacgcgagc atcatttttc gtcacctcct taaccgtcat
     8461 gcatcatgct ggcacgggcg cgcgcacgtt tatcccgctc gtatttttcg agcgcatcct
     8521 gtaactggcg gtcaagctgt gtccccggtg cagtaccacc cgtcaggctg atgtgatatt
     8581 cgtttttact ctggtccaca taagagcggc cagccggtgc cgtgaccggc tgataagcct
     8641 gatagcctgc ataagagctg gtcgccggaa tataaccacc ggtgccatac gtggcggcat
     8701 gagttctggc ggcggtctgg tcaagtgtgt ctgactcttt gttgatgacc ccgagctttt
     8761 ccagtaccca gtcaatgccg ctgcgcaatt tgttgaacgc attaagcggc agcatcagcg
     8821 cgtcagccag tgcctgcccg aacatgacgc ccgtgtcacg gcaacggttc agggtgtcct
     8881 gggtggcttt gaccggggca atcaggtttt taaaccactg ccacgcagcc tgtaactttt
     8941 cgcccagcca gtcaaacacc ggtttcagtg gcgtgaacag ctcccccacc ggcgcaaatg
     9001 ccgctttcag cccttcaacc acaccgccaa agaatgcgct gacaggctcc cagtatttac
     9061 ggataagcaa cgccccggcg acaatggcgg ccaccacagc cacaaccggc cagctaatcg
     9121 ccccgatggc cgtcataacg gcactaccaa ccgtcgtgaa gattgccccc attgcgcctg
     9181 ctgccgcgat gatggcattg atgccggtga taaccggcca ggctacaaga ccaattgcac
     9241 cgatgatgcc agtcagcgcc agcgcgccac cggcaatgag gccaatggtt gacgccagtg
     9301 atttgttttt ctgtacccag ccgtcgagtt ttaacacata ctttgtggcc gtctgcgtga
     9361 gcttacgtag cgcgccttcc tgctggtcaa acaggtctgt ccccaccgcc tcataagcgg
     9421 actgaaactc cttaaagtca ccgccgaggt tgtcctgcat gatatttacc agctctgcgg
     9481 tcttcccgtc tgaggcttta aacgcagcgg tcagtttgtc cagctttccg gttgacgcgg
     9541 cagtcatcag cacagcggcg gctgagctgg cctcctcccc gaaaatggtt ttcatgtatt
     9601 cggcctgctg ggcagtaccg agccggtttt tctcaaagct ggcctgcatt tctttcagaa
     9661 tggtaaatac tggtcgggtg tttcccttac tgtctgaggt tttcacgcca agctctttga
     9721 gtgcatccca tgcttttcca gtcggtgcct gcaggcgact taacacggca cggcttcccg
     9781 tccccgccat tgagcctgtg atttttgcat catgcagcgc cccgaccatt gcggcggttt
     9841 cttcaatgct gacaccggca ttttttgcca ctggtgcggc ataggtcagc gcatcgctca
     9901 tgccgtcaaa atcagcggcg gttttgttca tcgtcatgga gagaacatcc ccgatatgag
     9961 cgaccttatc gtttgaaagc tgaaaggcgg atttcatccc catcagcagg gcggcgtttt
    10021 cttccattgt gcggcggttc gccagcgcca tattcagcgt gaccggcgtt gccgcctgaa
    10081 tggcatcaac atccccaccc gctttcgcaa tgataatctg tgcaccggcc gcatcatccg
    10141 ctgaggcggc ggtgttgtca ccgagctggc gcgcctgctt gcggagtgca gccatttcgg
    10201 cggagtcttt tgccacaccg agcacagcct gcaattctga gtttttctgc gcaaactcat
    10261 aacctggcat cagcagctta actccggcta tcgttcccgc agcagcaatc cccacaccgg
    10321 cagcgcccac tgaggccata tttccggcca gttcctttcc ggcctgataa cgctgtttga
    10381 ctgcgttaag ttttgcctgt tgcgcactga cacgcgccag cgcgtcacgc tgccggttaa
    10441 gctgggcggt ggtttcactg atacggtttt tcagtccctg ctcatcatgt gcaagattgc
    10501 gggtattaat tcccacagcg gccagttccc gctgctggcg tttaacggaa tctgtcaggc
    10561 ggttatattt cgcctgtaag tcctccgccg cacgctttgc ggattccagc actttcgcct
    10621 gagcacgggt cgggcgttcg gtgtttttaa actgtgtggc aagggcttcg gcctcctgcc
    10681 gtgccttttc aagtgcatga ccagtcacgg cgagctgtgc actggtcttg cggaatccct
    10741 caatacggga tgcgtgaccg ttcagctcgc gcagtgattt ttgtgtttcc cggatatccc
    10801 ccgacagcaa cttactcgct ctgcggatgg atttaaacgg gcgggacgcc tggtcaacag
    10861 ccctgagcaa tacctgtaat tttacattgt tactcattcg tgtttccgct tcgcctgagc
    10921 gccttttcgc gccatgtgat gagttcggtc aggctcatgg gatacagttc tgatggcggc
    10981 cagtgaaata tcactgccac atccgccatc aggtcatcga ccgacagatt tttcggaagc
    11041 gtcactgcac cgagttcggc gacaaaaaac cgaccacctt accggccagc gccacaaggt
    11101 caggcagttc cagcgcggcg acttcctgct cggtcagcat cggtgccgtc atgcgcggca
    11161 gcaccttaat cagtgcatcg acttctgagt ttgcgaccgc agccagactg acaccgcgca
    11221 gcgtccccgc actgggtttc atcagcgtga cctgttcgat aacctgctca ccacgcttga
    11281 ccggattgtc cagggtaatc acattttctt tgttcatggt tttctcactt ctgaatcggg
    11341 gttaaccggt cagccaggct gaccggatga aaattacagg ccgatattgc ggcggtgttg
    11401 ctccagccgg tcgacgccgt tcaccttctc aatcatgttg atggtgtcga tttcgaccag
    11461 ctccttaccg tccatcgtca gccggaaata ggtgcagacc acggagattt tcgactcggt
    11521 gtcttctccc tgtttaccct cgccggtgtc gatttctttc tgacggccac gcatgaccac
    11581 ctctacggcc accgtttcgc cggtatcgtc acgctggtaa gagcctgcaa aacgaatcgg
    11641 cacggcatcc acaccggttg cggcgtaaag ctcccagata accgaatccg ggaagccccc
    11701 gagcgaccac tccattgaca gcgcatcgtc atcaaggccg aggtctaccg gtgcgcttcc
    11761 gttcatcccc gcaccgcgat agttttcgag tttacgggtc agttttggca gcgtgacgga
    11821 ctttgcaacg ccctgatagc tgtagccgtt cagaaagacg ttcattaact tgagtttgcg
    11881 cggcattgcc atcggtcagg ctccttaatt gctgttaacc gaggtgacca gatttgccag
    11941 gtatttatcg gtaatacgct ggcgcagggt caggttttca agaggaggca ccggggtata
    12001 gtcgtagtcg atatacagtt ttccggcctt gagggtttcc gcatcgttgg attcttcgct
    12061 gaaccagcag gtcgcatcca cgatatagcc gtttgttttc agctcacgga atttggcatt
    12121 gatgccgtca acgatgtcgc gaatcagcgt tgcggtgatg ggcttgtcca ccgcccacat
    12181 gtgcgcctca gccatcgtgt cggccagcac ctgcgcggtg cgggtgtagt tttcaaagag
    12241 gaacagcggg tcatcagagc aggtacggtt accccagaag cggaaaccgt cacggcgaat
    12301 cagcgttgtg acgcctgact cgttaagcag gtcagcatcg gtgccggact cctgcaaatc
    12361 ccagaatacg gacgcgctga tgccggtaac accgttcacc ccgacgttgg acagcgtttt
    12421 atgccagccc tgctcctggt cgattttagc gcgcagaccc agcgcacggg cggtggcata
    12481 cgcggtggcg gtggtactgg cgaccgtatc ccatgcgagg aaatccggcc agatgaccat
    12541 cagctcacgc tggctgaaat tctgacggta ggctttcacc tcggaaacgg tcttacagcc
    12601 ccatgcgctg atatacccga aagcgcgcag cttctgacag actgatgcca gtgcaacagc
    12661 cacctctttg gtgtccagtc ccggcacgcc gagaatacgc ggtttaacac cggttaccga
    12721 ctccgccgcc agcagggctt tcagtccggt gtactgaccg ttttcgtcgg tggtgccgat
    12781 gatattggaa acggtctgcg cgagtttcgt ttcctcgtcg tcgccggtgc cgtcttccac
    12841 acgcacgaca acggtgaccg gttttgactg gtcggcgatg gcctgcaacg atgccgccag
    12901 cgtgcctttt ttaccggcct ttgcaattgc gctctgcaca ttggtaatca gcaccggttt
    12961 attgaggggg aaggtttccg catccgcatc gctggccgtg cagaccatgc cgacaatggc
    13021 agtggatacg gtggaaatga cgcgggtgcc gtcgttaatc tccagcacct gcacgccgtg
    13081 atgatagtca ctcatccgtt taactccgtg gttaatgggt gcaactattt tctgttgggc
    13141 agtgcatgag acgctatttg acctggctgg tcagtggatg aaacaacaga taaagaaaat
    13201 gcgggcaatt cgcccgcatg tcctgatttg tacacactca ttttccgact gacagtttac
    13261 atagcccaaa cgctatcaaa tctgacagcc tgctatgagc gaaaagcgtt aaatctcacg
    13321 atatctgcta ccgggaaaaa tttcgattat cttcgatggg aactttgata atgtagtaaa
    13381 tgccattttc agcgatcact tttcaagcgt gtgtaaatga tta
//
LOCUS       DAZRUF010000106        13398 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZRUF010000106 DAZRUF010000000
VERSION     DAZRUF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.106"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            249..3035
                     /gene="polA"
                     /locus_tag="ACHXOL_002435"
     CDS             249..3035
                     /gene="polA"
                     /locus_tag="ACHXOL_002435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR6809396.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(3417..4049)
                     /gene="yihA"
                     /locus_tag="ACHXOL_002436"
     CDS             complement(3417..4049)
                     /gene="yihA"
                     /locus_tag="ACHXOL_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR6809397.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            4346..4512
                     /locus_tag="ACHXOL_002437"
                     /pseudo
     CDS             4346..4512
                     /locus_tag="ACHXOL_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4631..5140
                     /gene="yihI"
                     /locus_tag="ACHXOL_002438"
     CDS             4631..5140
                     /gene="yihI"
                     /locus_tag="ACHXOL_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR6809398.1"
                     /translation="MKPSSSNSRSKGHAKVRRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            5329..6702
                     /gene="hemN"
                     /locus_tag="ACHXOL_002439"
     CDS             5329..6702
                     /gene="hemN"
                     /locus_tag="ACHXOL_002439"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR6809399.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(6892..7002)
                     /locus_tag="ACHXOL_002440"
     CDS             complement(6892..7002)
                     /locus_tag="ACHXOL_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR6809400.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(7114..8523)
                     /gene="glnG"
                     /locus_tag="ACHXOL_002441"
     CDS             complement(7114..8523)
                     /gene="glnG"
                     /locus_tag="ACHXOL_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR6809401.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(8535..9584)
                     /gene="glnL"
                     /locus_tag="ACHXOL_002442"
     CDS             complement(8535..9584)
                     /gene="glnL"
                     /locus_tag="ACHXOL_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR6809402.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(9758..11167)
                     /gene="glnA"
                     /locus_tag="ACHXOL_002443"
     CDS             complement(9758..11167)
                     /gene="glnA"
                     /locus_tag="ACHXOL_002443"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR6809403.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            11540..13363
                     /gene="typA"
                     /locus_tag="ACHXOL_002444"
     CDS             11540..13363
                     /gene="typA"
                     /locus_tag="ACHXOL_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR6809404.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3378 a         3400 c         3520 g         3100 t
ORIGIN      
        1 tatctcaatt attagggatg gctttatttt taactgcatg aaaaacaaag acaaacatca
       61 tgctgtaaaa agcatgataa taaattaaaa gcgatgtaaa taatttatgc acaaagttat
      121 ccacatgacg atttgcgagc gatccagaag atctacaaaa gattttcacg aaaagcggtg
      181 aaaaactcat gttttcatcc tgtctgtggc atcctttacc cataatctga taaacaggca
      241 cggacattat ggttcagatc ccccaaaatc cacttatcct tgtagatggt tcatcttatc
      301 tttatcgcgc atatcacgcg tttcccccgc tgactaacag cgcaggcgag ccgaccggtg
      361 cgatgtatgg tgtcctcaac atgctgcgca gtctgatcat gcaatataaa ccgacgcatg
      421 cagcggtggt ctttgacgcc aagggaaaaa cctttcgtga tgaactgttt gaacattaca
      481 aatcacatcg cccgccaatg ccggacgatc tgcgagcaca aatcgaaccc ctgcacgcga
      541 tggttaaagc gatgggactg ccgctgctgg cggtttctgg cgtagaagcg gacgacgtta
      601 tcggtactct ggcgcgcgaa gccgaaaaag ccgggcgtcc ggtgctgatc agcactggcg
      661 ataaagatat ggcgcagctg gtgacgccaa atattacgct tatcaacacc atgacgaata
      721 ccatcctcgg accggaagag gtggtgaata agtacggcgt gccgccagaa ctgatcatcg
      781 atttcctggc gctgatgggt gactcctccg ataacattcc tggcgtaccg ggcgtcggtg
      841 aaaaaaccgc gcaggcattg ctgcaaggtc ttggcgggct ggatacgctg tatgccgagc
      901 cagaaaaaat tgctgggttg agcttccgtg gcgcgaaaac aatggcagcg aagctcgagc
      961 aaaacaaaga agttgcttat ctctcatacc agctggcgac gattaaaacc gacgttgaac
     1021 tggagctgac ctgtgaacaa ctggaagtgc agcaaccggc agcggaagag ttgttggggc
     1081 tgttcaaaaa gtatgagttc aaacgctgga ctgctgatgt cgaagcgggc aaatggttac
     1141 aggccaaagg ggcaaaacca gccgcgaagc cgcaggaaac cagtgttgca gacgaagcgc
     1201 cagaagtgac ggcaacggtg atttcttatg acaactacgt caccatcctt gatgaagaaa
     1261 cactgaaagc gtggattgcg aagctggaaa aagcgccggt atttgcattt gataccgaaa
     1321 ccgacagcct tgataacatc tctgctaacc tggtcgggct ttcttttgct atcgagccag
     1381 gcgtagcggc atatattccg gttgctcatg attatcttga tgcgcccgat caaatctctc
     1441 gcgagcgtgc actcgagttg ctaaaaccgc tgctggaaga tgaaaaggcg ctgaaggtcg
     1501 ggcaaaacct gaaatacgat cgcggtattc tggcgaatta cggcattgag ctgcgtggga
     1561 ttgcgtttga taccatgctg gagtcctaca ttctcaatag cgttgccggg cgtcacgata
     1621 tggacagcct cgcggaacgt tggttgaagc acaaaactat cacttttgaa gagattgcgg
     1681 gtaaaggcaa aaatcaactg acctttaacc agattgccct cgaagaggcc ggacgttacg
     1741 ccgccgaaga tgcggatgtc accttgcagt tgcatctgaa aatgtggcca gatctgcaaa
     1801 aacacaaagg gccgttgaac gtcttcgaga atatcgaaat gccgctggtg ccggtgcttt
     1861 cacgcattga acgtaacggt gtgaagatcg atccgaaagt gctgcacaat cattctgaag
     1921 agctcaccct tcgtctggct gagctggaaa agaaagcgca tgaaattgca ggtgaggaat
     1981 ttaacctttc ttccaccaag cagttacaaa ccattctgtt tgaaaaacag ggcattaaac
     2041 cgctgaagaa aacgccgggt ggcgcgccgt caacgtcgga agaggtactg gaagaactgg
     2101 cgctggacta tccgttgcca aaagtgattc tggagtatcg tggtctggcg aagctgaaat
     2161 cgacctacac cgacaagctg ccgctgatga tcaacccgaa aaccgggcgt gtgcatacct
     2221 cttatcacca ggcagtaact gcaacgggac gtttatcgtc aaccgatcct aacctgcaaa
     2281 acattccggt gcgtaacgaa gaaggtcgtc gtatccgcca ggcgtttatt gcgccagagg
     2341 attatgtgat tgtctcagcg gactactcgc agattgaact gcgcattatg gcgcatcttt
     2401 cgcgtgacaa aggcttgctg accgcattcg cggaaggaaa agatatccac cgggcaacgg
     2461 cggcagaagt gtttggtttg ccactggaaa ccgtcaccag cgagcaacgc cgtagcgcga
     2521 aagcgatcaa ctttggtctg atttatggca tgagtgcttt cggtctggcg cggcaattga
     2581 acattccacg taaagaagcg cagaagtaca tggaccttta cttcgaacgc taccctggcg
     2641 tgctggagta tatggaacgc acccgtgctc aggcgaaaga gcagggctac gttgaaacgc
     2701 tggacggacg ccgtctgtat ctgccggata tcaaatccag caatggtgct cgtcgtgcag
     2761 cggctgaacg tgcagccatt aacgcgccaa tgcagggaac cgccgccgac attatcaaac
     2821 gggcgatgat tgccgttgat gcgtggttac aggctgagca accgcgtgta cgtatgatca
     2881 tgcaggtaca cgatgaactg gtatttgaag ttcataaaga tgatgttgat gccgtcgcga
     2941 agcagattca tcaactgatg gaaaactgta cccgtctgga tgtgccgttg ctggtggaag
     3001 tggggagtgg cgaaaactgg gatcaggcgc actaagattc gcctgaacat gccctttttt
     3061 cgtaagtaag caacataagc tgtcacgttt tgtgatggct attagaaatt cctatgcaac
     3121 aactgaaaaa aaattacaaa aagtgctttc tgaactgaac aaaaaagagt aaagttagtc
     3181 gcgtagggta cagaggtaag atgttctatc tttcagacct tttacttcac gtaatcggat
     3241 ttggctgaat attttagccg ccccagtcag taatgactgg ggcgtttttt attgggcgaa
     3301 agaaaagatc cgtaatgcct gatgcgctat gtttatcagg ccaacggtag aattgtaatc
     3361 tattgaattt acgggccgga tacgccacat ccggcacaag cattaaggca agaaaattat
     3421 tcgccgtcct gcgtttcttc tacaggctgc atctcgctaa accaggtatc cagtttctgc
     3481 cgcagcttgt ccacgccttg tttcttcaac gaagaaaacg tttcaacctg cacatcaccg
     3541 ttaaacgcca gtacagcttc acgcaccata ttcaattgcg ctttacgtgc gccgcttgcc
     3601 agtttgtccg ctttggtcag cagcaccaga acggcgatat tgctgtctac cgcccactca
     3661 atcatctgct gatccaaatc tttcagcgga tggcgaatat ccattagcac caccagacct
     3721 tgcaggctct gacgtttttc gaggtattcg ccgagcgcac gctgccattt gcgcttcatc
     3781 tcttccggga cttccgcata accgtaccca ggcaagtcaa ccagacgctt gccgtcagcc
     3841 acttcaaaca ggttgataag ctgggtgcgc cctggggttt ttgaggtacg agccaggctt
     3901 ttctggttag tcagcgtgtt cagcgcgctg gatttacctg cgttggaacg gcctgcaaaa
     3961 gccacttcaa ttccggtatc ggaaggtagg tggcgaatat caggcgcact catcacaaaa
     4021 tgcgtctgtt gataattcaa attagtcaaa gcggtcgtct ccgtcagtca aagctttgtg
     4081 gcgattatac ctgaaccata ataaaaggcg gatttttcgg cgtgagcgtt gtaagtaaaa
     4141 gccatacgct ttgtgagaca ttgccgatag tctttatgcg aaatagcaga gaaaattctg
     4201 cgatgcatgt caaataagct atataaatca gtgaattgct tttatataaa actctgaaaa
     4261 tgtgcaaata ctgatggcgg ttgattgttt gtttaaagca aaggcgtaaa gtagcaccca
     4321 tagagcgagg acgctaacag gaacaatgac tcaggatgag ggtcaggagc gccaggaggc
     4381 gaagacagag gattgtcagg aagacaaacg tccggagacg taattaaacg gaaatggaat
     4441 caacacggat tgttccggct aaaggaaaaa cagggtgtgt tggcggcctg caaggattgt
     4501 aagacccgtt aagggttatg agtcaggaaa aaaggcgaca gagtaatctg tcgccttttt
     4561 tctttgcttg ctttctgtta gattccgccg caaatctata ctgaataaaa cggctaaaag
     4621 acgaaccatt atgaaaccat catcttcaaa ctcacgcagc aaaggtcatg caaaagtgcg
     4681 tcgaaaaaca cgcgaggagc tggatcagga agctcgtgat cgcaagcgtc agaaaaaacg
     4741 tcgtggtcat gcgccgggca gccgcgcagc gggcggaaac accacgtcag gcagcaaagg
     4801 ccagaacgca ccaaaagatc cacgtattgg cagtaaaacc cctattccat tgggcgtggc
     4861 tgaaaaagtc accaaacagc acaaaccgaa gagtgagaaa cctatgcttt caccgcaggc
     4921 ggagttggaa ttactggaaa cggatgagcg tctggatgcg ctgctggaac gtctggaagc
     4981 aggcgaaacc ctgagcgccg aagagcaatc ctgggtggat gctaaactgg atcgtattga
     5041 tgagttgatg cagaaactcg gcctctctta tgacgatgac gaagaagagg aagaagacga
     5101 gaagcaagaa gacatgatgc gtctgctgcg gggtaactaa cggattgcca ccgtgggcct
     5161 tcccgttctg ctgataaccc ttccggttat atgttatctg gtgtggttat tcgttaaact
     5221 acagcggttg tcgcggcgac aaaagtggct gcgcaaccgg ctgatgaccc gaaacgggca
     5281 tccgccggta cgccgtagcc gccagagacg ccatcggaag gagtgagcat gtctgtacag
     5341 caaatcgact gggatctggc cctgatccag aaatataact attccgggcc acgatacacc
     5401 tcgtacccga ccgcgctgga gttttcagaa gacttcggcg aacaggcgtt tttacaagcc
     5461 gtggcgcgct atcctgagcg tccattatct ctctacgtac atatcccgtt ctgccataag
     5521 ctttgttact tctgcggttg caataagatt gttactcgcc agcagcacaa ggccgatcag
     5581 tatctggacg cgctggagca agaaatcgtc catcgtgcac cgctgttcgc cgggcgtcac
     5641 gtcagccaat tgcactgggg cggcggaacg ccgacgtatc tgaataaagc gcaaatcagc
     5701 cgcctgatga agctgctgcg cgaaaacttc cagttcaatg ccgatgcgga gatttcgatc
     5761 gaagtcgatc cgcgggaaat cgaactggat gtactcgatc atttacgcgc cgaaggcttt
     5821 aatcgcctga gcatgggcgt gcaggacttc aacaaagaag tgcaacgtct ggttaaccgc
     5881 gagcaggatg aagagttcat ctttgcactg cttaaccatg cgcgtgagat tggttttacc
     5941 tccaccaaca tcgacctgat ttacggcctg ccgaaacaga cgccggagag tttcgccttt
     6001 accctgaaac gtgtggcgga gctgaacccc gatcgtctga gtgtctttaa ctacgcgcat
     6061 ctgccgacca tttttgctgc tcagcgcaaa atcaaagatg ctgacctgcc gagtccgcag
     6121 caaaaactcg atatcctgca ggaaaccatc gccttcctga cgcaatcggg ctatcagttt
     6181 atcggtatgg atcactttgc ccgtccggat gacgagctgg cggtggccca gcgtgaaggc
     6241 gtgctgcatc gtaacttcca gggctacacc actcagggcg ataccgatct gctggggatg
     6301 ggcgtttccg ccatcagcat gattggcgac tgctacgcgc agaaccagaa agagttgaag
     6361 cagtactatc agcaagtgga tgaacaaggc aatgcgctgt ggcgtggtat tgcgctaacg
     6421 cgtgatgact gtattcgccg cgatgtgatt aagtcgctca tctgcaactt ccgtctggat
     6481 tacgccccta ttgagaaaca gtgggatttg cacttcgctg attactttgc ggaagatctc
     6541 aagctgctcg ccccgttagc aaaagatggg ctggtggatg tggatgagaa gggaatacag
     6601 gtgacggcga aaggtcgctt gctgatccgc aacatttgca tgtgctttga tacctatctg
     6661 cgccagaaag cgcggatgca gcagttctca cgggtgattt aaatagtgaa tggcgcttcg
     6721 tttacaaagt agaaaacgaa agcgccatca atgtgctgag aacaagattg cctgatgcgc
     6781 tacgcttatc aggcctacaa aacctgttga aaatattgct tttgtaggcc ggataaggcg
     6841 ttcacgccgc atccggcatg aacaaagagc acttcatcag caatctgaaa ctcaactaaa
     6901 cagcccaatc attgcggcac acagcacggc agcaacggca gtttgcggtg tgtggctgtc
     6961 aactgcgcca ctcgatacca gattaattat tgattccagc ataatgactc tccccgtttt
     7021 ccgggcaaga tcatactgaa cttatcggaa cagtaaagcg taaaatacca gcaatttgcg
     7081 ctcaataatc aatctttaca cacaagctgt gaatcactcc atccccagct cttttaactt
     7141 acgcgtcagg gtgttgcggc cccagccgag tagccgcgcc gcttcctgtt tatgcccctg
     7201 cgtatgtcgc aacgcggtcg tcagtaacgt ccgctccagc tctggctgcg cttcggaaag
     7261 cagattttga tgaccggaac gcagcgctct gtctgcccac tgcgcaagaa gcgttgccca
     7321 gctgtccggt tgcatttgcg aagtactctc cgcaaccgtt gattcaaaca gttcgccggg
     7381 caaatcctga atcaacactt cctgcccggc ggccatcacc gttagccagc ggcaggtgtt
     7441 ttccagctgg cgcacgttgc ctggccacgc cagacgcgtc agagcagctt cggtttccgg
     7501 atgcagtaac ttcgcttcta cgcccagttc gcgcgcggca acctgtaaaa aatggcgcgc
     7561 cagacgggga atatcttccc gacgttcgcg cagcggcggc agatgaacgc ggataacgtt
     7621 caggcggtgg aacagatcct cacggaactt accttcctgc actcgttgtt cgagattctg
     7681 gtgagtggca gcgataatac gcacatccac tttcaccggc gcatagccgc caacgcggta
     7741 aaactgaccg tctgccagca cgcgcagcaa acgcgtctgc acatccagcg gcatatcacc
     7801 aatttcgtcg aggaataatg taccgccatc ggcctgttca aaacgcccct gacgaatggt
     7861 attcgcgcca gtaaacgcgc ctttctcgtg gccaaacagt tctgattcga tcaaatcttt
     7921 tgggatagct gccatattca gcgcgataaa cggcgctttg gcgcgcggac tgtggcgatg
     7981 cagggcatga gcgaccagtt ctttaccggt gccggattcg ccgttaatca gcacgctaat
     8041 agaagaacgc gaaagccgac cgataatacg gaacacgtcc tgcatggctg gcgcttcgcc
     8101 gatgatatcg gtcgttgggc cgttaagctg aatattacgc ggctgctgct gttcctggta
     8161 atgactgata gcgcgctcaa ccagcgccac tgcttcgtcg atatcaaacg gtttgggcag
     8221 ataatcaaac gccccttgtt gataggcgct gacggcagca tccagatcgg aatgtgcggt
     8281 cataatgatg accggaagca ttggatggcg ctgtttaatc tgcttgagca gcgccagccc
     8341 gtccattccc ggcatacgga tatctgaaag cagcacatcc ggcgttttgc tcgccagcgc
     8401 ctccagtact tccgcgccgt tctcaaatgt cgtacaggtt aaacccgctc cagcgagcgc
     8461 acgttcaagc acccaacgga tggaactatc gtcatcgact acccagacta tccctcgttg
     8521 cataaacgtc acctttattt cctgataggc aggtaaaccg agaactcggt atgacctggc
     8581 caactggtaa attcaatttt gcctgaatgc tgatcaatca aattacgggc gatggataag
     8641 ccaagcccgg tgccaccttc gcggccgctg accatcgggt aaaacagcgt atcctgcaaa
     8701 tgaggcggaa tgcccggccc gttatcttcc acatcaatcc gcgccgccag ccggtagcgc
     8761 tcgccgtgta aggtcagttg aaacgcggtg cgggtacgca gaatgatttc accgccttcc
     8821 ggccccagcg cctgtagcgc attgcgcaca atattcagca agacctgttc aatttgatcc
     8881 gggtcgtgcg ccagttccgg caggctgggg tcgtaatcac gaatcaaccg cacgttgtcc
     8941 ggcagttcca tcgacaccag cgtcaccacg cgttcagcta ctttgtgaat actttcggta
     9001 acgcgcgtac cgggcagctg cggccccaac agacggtcga ccagatttcg cagccggtcc
     9061 gcctgttcga taatcacttt ggtatattcg agtagtgatg ggtcaggtaa cgctttgctg
     9121 agcagctgcg ccgcgccacg taaacctcca agcggatttt taatctcatg tgccaggccg
     9181 cgcactaaat cacgggcagc aacctgctgg gcgtgctgta gctgttcctg acttaagcgg
     9241 cgctggttat ccatcggagc catctccagc aggatcatgc cgtccggcat acgctgggcc
     9301 gtcacagaaa ggatatgcga gcgcccgtcg atgaccagcg tcacttcgtt atcggtaaaa
     9361 ccttgccccg cctccagact ttcttgcatc agctcgatat ttaatgagaa gtagctcaac
     9421 agttccggta acggtgtacc aaacaatttg cgggagcttt gggcgagcag ttgttgcgcg
     9481 gcggggttgg cgtaatggat cgccagattg tcatcaatta acaaaatact gttaatcagc
     9541 gagttgagga tctgcccagc atcgggctgc gtgcctgttg ccataaagca gtctcctgaa
     9601 caggttgcac cattttagtg cattatagct ttttacggat aaaaagcgcg aagcatcaga
     9661 gaattgacgg agaaaaaagc ccatgcagag atgggctgaa agtttccacg gcaactaaat
     9721 cccggcgttg ttgcgccggg tagtacaact tcaactatta gacgctgtag tacagctcaa
     9781 actctaccgg atgcggagtc atacgcacgc ggtcatcttc ttcgcgacgt agagcgatgt
     9841 acgcatcaat tgcttcgtca gtgaacacgc caccggcttt caggaactcg cggtccagat
     9901 ccagttcgtt cagtgcttct tccagagagc ctgcaacctg tgggatctct ttcgcttctt
     9961 ctggcggcag gtcatacagg tttttgtcca tggcttcgcc cggatggatc ttgttcttga
    10021 taccatcaag accggccatc agcagggcag caaagcacag gtacgggtta gctgccggat
    10081 ccgggaaacg tacttcgata cgacgtgctt tcggagaaga aaccaccgga atacggatag
    10141 acgcagaacg gttacgcgca gagtaagcca gcattaccgg tgcttcatag cccgggacca
    10201 gacgcttata agagttggtg gtcgggtttg ccagggcgtt aatcgcttta gcgtgtttga
    10261 ttacgccgcc aatgtagtac agcgcctgct cagacagacc tgcgtatttg tcgcctgcga
    10321 acaggttaac gccgttttta gacagagaca tgtggcagtg cataccggag ccgttatcac
    10381 cgaacatcgg ttttggcata aaggtcgcgg ttttaccgaa gcggtgcgct acgttgtgca
    10441 caacatattt gtagatctga atttcgtcag cttttttggt catggtattg aagcgggtag
    10501 ccacttcgtt ctgaccagca gtcgctactt cgtggtgatg ggcttcaacc accagaccca
    10561 tctgttccat caccagacac atttcagaac gaatatcctg agccgagtct accggtggaa
    10621 ccgggaagta accgcctttc actgccggac ggtgaccttt gttaccacct tcgtattggg
    10681 tggaggagtt ccatgcgcct tcgatatcgt cgatagcaac gtgggaaccg gagatagatg
    10741 atccgaaacg gatgtcatcg aacaggaaga attcaggttc tggcccgaac agtacggtgt
    10801 cggcaatgcc agtggaacgc aggtaatctt cggcgcgctt cgcaatggag cgcgggtcac
    10861 ggtcatagcc ttgcagggtg ccaggttcaa ggatgtcgca acggataatc agggtggagt
    10921 cggcgaagaa cgggtcaatc actgcggtgg atgcgtctgg catcagcacc atgtcggact
    10981 cgttaatgcc tttccagccg ccaatcgagg agccgtcaaa cattttgcct tcttcgaaga
    11041 attcagcatt cacctgatga gcagggatag tgacgtgctg ttctttacct ttagtatcgg
    11101 tgaagcgcaa atcaacaaac ttcacttcgt gctcgttcag catcgtcagt acgtgttcag
    11161 cggacatact ttaactctcc tggattggtc atggtcgtcg tggtaacgaa atctgcaatt
    11221 attttggccg tgtcgccgta aaaaagataa agcgaaatct gtgccaactt ttaaattgcc
    11281 cctaaaaggc gttatcatgc gcaccatcgt gcaaaagggc tgcaccacga tgtgaatgtt
    11341 gcaccaatat agtgcttcaa tggaaacatt aagcaccatg ttggtgcaat gacctttgga
    11401 taaccctttt tatgctccgt gaaagcgatc acaaagggac tctgcaatac ttgtttgcgg
    11461 aggatgtttg tgatcctgtt ttgtagtgcg attaatccgt gtacaataac gcgctatttc
    11521 taatgcctga ggcaaagttg tgatcgaaaa attgcgtaat atcgccatca tcgcgcacgt
    11581 agaccatggt aaaaccaccc tggtagacaa gctgctccaa caatccggta cgttcgactc
    11641 tcgtgccgaa acccaagagc gcgtgatgga ctccaacgat ttggagaaag agcgtgggat
    11701 taccatcctc gcgaaaaaca ccgctatcaa atggaatgat taccgtatca acatcgttga
    11761 taccccgggg cacgccgact tcggtggtga agttgaacgt gtaatgtcca tggtagactc
    11821 agtgctgctg gtggttgacg catttgacgg cccgatgccg caaacgcgct tcgtaaccaa
    11881 aaaagcgttt gcttacggcc tgaagccgat tgttgttatc aacaaagttg accgccctgg
    11941 cgcgcgtcct gattgggttg tggatcaggt attcgatctg ttcgttaacc tcgacgcgac
    12001 cgacgagcag ctggacttcc cgatcgttta cgcttctgcg ctgaacggta tcgcgggtct
    12061 ggaccacgaa gatatggcgg aagacatgac cccgctgtac caggcgattg ttgaccacgt
    12121 tcctgcgccg gacgttgacc ttgacggtcc gttccagatg cagatttctc agctcgatta
    12181 caacagctat gttggcgtta tcggcattgg ccgcatcaag cgcggtaaag tgaagccgaa
    12241 ccagcaggtc actatcatcg atagcgaagg caaaacccgc aacgcgaaag tcggtaaagt
    12301 gctgggccac ctcggtctgg aacgtatcga aaccgatctg gcggaagctg gcgatatcgt
    12361 ggcgatcacg ggccttggcg aactgaacat ttctgacacc gtttgcgaca cgcaaaacgt
    12421 tgaagcgctg ccggcactct ccgttgatga gccgaccgtt tctatgttct tctgcgttaa
    12481 cacctcgccg ttctgcggta aagaaggtaa gttcgtaacg tctcgtcaga tcctggatcg
    12541 tctgaacaaa gaactggtac acaacgttgc gctgcgcgta gaagaaaccg aagacgccga
    12601 tgcgttccgc gtttctggtc gtggcgaact gcacctgtct gttctgatcg aaaacatgcg
    12661 tcgtgaaggt ttcgaactgg cggtatcccg tccaaaagta atcttccgtg aaatcgacgg
    12721 tcgtaaacaa gagccgtatg aaaacgtgac tctggacgtt gaagaacagc atcagggttc
    12781 tgtaatgcag gcgctgggcg aacgtaaagg cgacctgaaa aacatgaatc cagacggtaa
    12841 aggccgcgta cgtctcgact acgtgatccc aagccgtggt ctgattggct tccgttctga
    12901 gttcatgacc atgacttccg gtactggtct gctgtactcc accttcagcc actacgacga
    12961 cgtacgtccg ggtgaagtgg gtcagcgtca gaacggcgta ctgatctcta acggtcaggg
    13021 taaagcggtc gcgttcgcac tgttcggtct gcaggaccgc ggtaagctgt tcctcggtca
    13081 cggtgcagaa gtttacgaag gtcagattat cggtattcac agccgctcta acgacctgac
    13141 tgtaaactgc ctgaccggta agaaactgac caacatgcgt gcttccggta ctgacgaagc
    13201 tgttgttctg gttccgccta tccgcatgac tctggaacaa gctctggagt tcatcgatga
    13261 tgacgaactg gtagaagtga ctccgacctc tatccgtatt cgtaaacgtc acctgacgga
    13321 aaacgatcgt cgtcgcgcca accgcgcacc gaaagacgat taatttcgtt ttttagttat
    13381 aaaaaacctg ccatcgct
//
LOCUS       DAZRUF010000107        13376 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZRUF010000107 DAZRUF010000000
VERSION     DAZRUF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.107"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..81)
                     /locus_tag="ACHXOL_002445"
     CDS             complement(<1..81)
                     /locus_tag="ACHXOL_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR6809405.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTL"
     gene            complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHXOL_002446"
     CDS             complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHXOL_002446"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR6809406.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHXOL_002447"
     CDS             complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHXOL_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR6809407.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHXOL_002448"
     CDS             complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHXOL_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR6809408.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5734..6231
                     /gene="msrC"
                     /locus_tag="ACHXOL_002449"
     CDS             5734..6231
                     /gene="msrC"
                     /locus_tag="ACHXOL_002449"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR6809409.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6328..7026
                     /gene="proQ"
                     /locus_tag="ACHXOL_002450"
     CDS             6328..7026
                     /gene="proQ"
                     /locus_tag="ACHXOL_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR6809410.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7046..9094
                     /gene="prc"
                     /locus_tag="ACHXOL_002451"
     CDS             7046..9094
                     /gene="prc"
                     /locus_tag="ACHXOL_002451"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR6809411.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9286..10167
                     /gene="htpX"
                     /locus_tag="ACHXOL_002452"
     CDS             9286..10167
                     /gene="htpX"
                     /locus_tag="ACHXOL_002452"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR6809412.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10213..11276)
                     /locus_tag="ACHXOL_002453"
                     /pseudo
     CDS             complement(10213..11276)
                     /locus_tag="ACHXOL_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHXOL_002454"
     CDS             11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHXOL_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR6809413.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12387..12626)
                     /locus_tag="ACHXOL_002455"
     CDS             complement(12387..12626)
                     /locus_tag="ACHXOL_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR6809414.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHXOL_002456"
     CDS             12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHXOL_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR6809415.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13003..13290
                     /locus_tag="ACHXOL_002457"
     CDS             13003..13290
                     /locus_tag="ACHXOL_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR6809416.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3475 a         3305 c         3455 g         3141 t
ORIGIN      
        1 taacgttcga tcacccggcg attcaccgtg taattcgccg tcgtcgattt caaaagcgcc
       61 tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccggttg tggtacgacc
      121 agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag tcaaaaagtg
      181 cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc
      241 acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg
      301 ctggaaagta accaacacgt catccgccac tggcgcggct tgcgggtaaa catcgcgccc
      361 gcgataccac tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat tgtcgggggt
      421 ggcaagggca ataactgctt catgctgcca gcgataacct ttgttgtgcg tttcggcaag
      481 tttaatcccc aagcgagaaa atcggacttt accgatcagg gcttcaatgc ccaccgggaa
      541 caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt ttaagccaac
      601 acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct ttcactgggc tgaacggaaa
      661 attaccgact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg
      721 agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc
      781 ggtcagcgcc ttgtttgcac cagggaagag atcaccaagc ggtaaaaact ctactgcgtc
      841 ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca
      901 ggtcgagtaa accagcgtac cgccagggcg taatgcatga aaggcgctgt cgatcagttc
      961 ccgttgggta gctgcgattt cctgattgct ttctggtgac cagtttttta gcgcatcggg
     1021 atctttacgc accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcaaacat
     1081 ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa cattactgat
     1141 accacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc
     1201 aagaattgcc ccttcgttat tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc
     1261 ggcagcgaca tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcgacggg
     1321 caacattgaa ctggcttcct gaatataaaa caggccactt aaatgctcgg cggtactacc
     1381 caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat
     1441 tggcgtaagc gtccagccat aaggagcggt taattgcagg aaatcagcaa cggagatttt
     1501 cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc
     1561 aaaagagagc gtcgaaggca tcgcttcgcg catttgcgtc agaaaggcgt ccgggaaata
     1621 aacggtgtgt tgggccacga gcatatacca ctggcaaaaa aaacaggcgc gcagtttagc
     1681 ataaacgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagtaccc
     1741 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgcttgcc ttcctgggct
     1801 ttcggtgcca gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgg
     1861 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     1921 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     1981 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2041 agaccacgga acagcacagg cgtaccaata cctaacgaac cggcttccgg tgcttcgaca
     2101 ataatgctta agccatccag gtaacgcgaa tcagtaatag tagcttcttg caactcaaag
     2161 tcacggcgag gattaccccg acctggttcg acattgatat acggctggag gatagtatct
     2221 agatgctcaa cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg
     2281 cgggcgaagg tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc
     2341 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     2401 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca
     2461 cgggccgctg tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg
     2521 gcaccgctga ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc
     2581 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     2641 ctattgctgg tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg
     2701 ttagctcgcg gacgcacggt aatcacttca ccaacttcaa atttacggta cagcactacc
     2761 gagcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     2821 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     2881 atctcacctt tatcgcctgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     2941 gcaccaagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3001 aatttgctat cgccttttac cagttcgcga tgctgaggct cgatggcgac ggtaaaggta
     3061 acgcctttgc tggtgagttt acgatcaata acctggccta cctgcacgcc gtgaagaatg
     3121 agcggctgac cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca
     3181 tcaggttcat gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg
     3241 ccatcgccgg gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg
     3301 ctaagggata atttcggatt aagtaattcg atgcgggtat tttcacgaag cagggtaacg
     3361 acgctgggat taactgtcat ttccccagtg actttaccac caggatttaa atccagttta
     3421 gttagctgtc cgacttccag tccctgatac attaatggcg tcgagtcggc ggttaatccg
     3481 gccccactcg gcagttccag tttgattatt acgccacgct ggctatgggc gaggtcttca
     3541 tacagaccaa aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca
     3601 atcgcaccgt taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgatactg
     3661 acgttggcat caacgccgga aacgttccag aaacggctac cttttttcac caggtcggta
     3721 aaacgccgct cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag
     3781 tcgtagactt ttcccacagg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa
     3841 ccgagatcgg gggcttgcag gtggatcatc agatcgccat tgtccagtcg atatttcggt
     3901 tgggtatcga gtgcgacaaa gtgatcctgc tctttacctt tacctggcat catgccgata
     3961 tagttaccac cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc
     4021 accagccaga actgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc
     4081 ttgacttcaa tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca
     4141 acgccctgat aacgaacagg cgtacggccc gggacaatac catccgccga cataaagtcg
     4201 atggtgacgg tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt
     4261 agcgcgatga aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct
     4321 tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag
     4381 ccattccaca gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc
     4441 catagtaaaa gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa
     4501 caggtcgagc atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt
     4561 gcgtaaacct tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac
     4621 tttagtaaac ggtaccagaa tactggcgat aaacacgatt ccggcgaccg caatgttgct
     4681 acttgccagc gacataatcc cggaaagaat cgtatcttcc tgccggccac cattcagata
     4741 aatgatagaa ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc
     4801 cgcccagcat ttttgcaaac tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg
     4861 gcagcgacca cgctgatcag gatagccggt aaaatggcac ccaagacaga cacgaagttt
     4921 ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt
     4981 aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac
     5041 acccgcctgg atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag
     5101 gtagatatcc agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa
     5161 attcattccc agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg
     5221 ggcaccaata acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat
     5281 ttgccagatg ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa
     5341 cagcggttcg ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag
     5401 gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc
     5461 actctgatga gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg
     5521 gtaatcacca cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt
     5581 aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatctt
     5641 aacccatgat gggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg
     5701 cagttccagg taagacaaac aagtgattat ataatgaaca aaacagaatt ttacgcggat
     5761 ttaaatcgcg actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg
     5821 aacaccagtg cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg
     5881 cttgaggacg atacactggt actcggacca tttcagggca aaattgcctg tgtccggata
     5941 cccgtcgggc gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag
     6001 gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt
     6061 ctgccgctgg tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc
     6121 ggtcgcttta cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa
     6181 gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat
     6241 aacgtagcat ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg
     6301 ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa
     6361 gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg
     6421 cgtccgctga aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac
     6481 ctgagcaaaa cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt
     6541 tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg
     6601 gacgagcaac atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag
     6661 gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa
     6721 gaagacgcac cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc
     6781 gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca
     6841 cctcgcgaag aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc
     6901 ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagat
     6961 ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg
     7021 ttctgaaacg gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg
     7081 cctgcttgca atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat
     7141 tccggtatta aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt
     7201 cacccgttct cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga
     7261 ccgctacctg aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca
     7321 gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt
     7381 ctacgatctc tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc
     7441 ggtactggaa aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa
     7501 agcgccctgg ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt
     7561 cgacgagtta agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac
     7621 acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc
     7681 gctggcaatg acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg
     7741 taataccgaa cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct
     7801 gcaaatggat gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa
     7861 gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat
     7921 ggttgacgtg attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg
     7981 cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac
     8041 attgacccgt gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt
     8101 cggtaaagag aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga
     8161 tgtcaaagtt caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct
     8221 gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc
     8281 tgcgggtccc attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac
     8341 cgacgggcag gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc
     8401 ggcttcagaa atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga
     8461 accgacgttt ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca
     8521 gatgttacgt cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta
     8581 tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac
     8641 gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag
     8701 cattgatgcc gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct
     8761 gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat
     8821 cgcgcgcttc aacgctatga gggacaagcg caatatcgtt tctctgaact acgctgtgcg
     8881 tgagaaagag aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa
     8941 acgcgaaggt aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc
     9001 ggatccttat ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc
     9061 cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc
     9121 ctgatttttt aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt
     9181 ctttctggtg acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg
     9241 ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg
     9301 ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca
     9361 gggatacagt cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt
     9421 ggttccttcg tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa
     9481 gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag
     9541 gctcgtcagg cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac
     9601 gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg
     9661 cagaacatga gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc
     9721 aatggtgata tggtcaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt
     9781 atttcccgta ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt
     9841 gaagagagca acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg
     9901 tttggtattc tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct
     9961 gatgccggtt cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg
    10021 aaaaccagct atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag
    10081 tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc tggataaacg aattgaagct
    10141 ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc
    10201 gacgcgcttt ttttatgccc tggatcgtgg ctgagtgata cgtaaaccac tgacacaggc
    10261 agcaatcact gccagaatag ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa
    10321 ctgatttagc atcagcgcca ccagtgccgc gccgctactc tgacccagta gacgagccgt
    10381 tcctaacatg ccactggctc caccgctacg ttcgcgagga gcggaggtaa taatggtgtg
    10441 gttattgggt gactggaaca agccaaatcc agcgccacat aagatcatcg gccagataat
    10501 attgatatcc gcaggtgacg cgggcagcag aaccagggga aaaagccccg cagccatgat
    10561 gaacaaccct agagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccaa
    10621 cggagccatc accatcgttg ctaacggcca cggtgtcaga agtaaacctg tttcgacttc
    10681 actacgcccg agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc
    10741 gcagaaagag caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc
    10801 caccggtagc agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac
    10861 aaccattacc accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact
    10921 caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa
    10981 acggggttta ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc
    11041 gataatacct aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat
    11101 tgctgcagca attgtcggcc cggcagcaga agagacggca acaatatggc gattgttggc
    11161 agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaatgccaat cacaatggtt
    11221 aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt tggcatagga
    11281 attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat attcacggtg
    11341 gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct ggtgcgaaat
    11401 tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat aaatggctaa
    11461 cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt ttggcatttt
    11521 gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat
    11581 gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt atgtggcgca
    11641 ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg gcgctcgcgc
    11701 gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga tctcccgcct
    11761 gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt acattcacaa
    11821 aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta atccgctgta
    11881 cagcaccgcg attggtaagg tactgctggc atggcgcgat ctcgatgaag tgaagcaaat
    11941 tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta cagaagcgtt
    12001 attacccgtt ctggaccagg tgcgcgagca ggggtatggc gaagataacg aagagcagga
    12061 agaagggctg cgatgcattg cggtaccggt atttgatcgc tttggcgtgg tcattgccgg
    12121 tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc
    12181 aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg actatccgtt
    12241 ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt tagcatgaga
    12301 aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat tttactcatg
    12361 agtgagaatc tggttgttta ttacccttaa ccattatcga ccacgatatt gcttttacgt
    12421 aacagtgggc aatctgttat ccccaagaaa ccacttttag tgtgcaagta ttgtaccgtg
    12481 ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc attctctttg
    12541 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
    12601 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
    12661 tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat atcgacatag
    12721 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aagcggagat
    12781 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
    12841 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
    12901 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
    12961 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
    13021 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc
    13081 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
    13141 gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca
    13201 aacgaaccag agaaagcaga taaaaagacc attgagagtc aaaaatcggt tgatgacgaa
    13261 gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt
    13321 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcttca
//
LOCUS       DAZRUF010000108        13320 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZRUF010000108 DAZRUF010000000
VERSION     DAZRUF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.108"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(67..771)
                     /locus_tag="ACHXOL_002458"
     CDS             complement(67..771)
                     /locus_tag="ACHXOL_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR6809417.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(908..1360)
                     /gene="moaE"
                     /locus_tag="ACHXOL_002459"
     CDS             complement(908..1360)
                     /gene="moaE"
                     /locus_tag="ACHXOL_002459"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR6809418.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1362..1607)
                     /gene="moaD"
                     /locus_tag="ACHXOL_002460"
     CDS             complement(1362..1607)
                     /gene="moaD"
                     /locus_tag="ACHXOL_002460"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR6809419.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1600..2085)
                     /gene="moaC"
                     /locus_tag="ACHXOL_002461"
     CDS             complement(1600..2085)
                     /gene="moaC"
                     /locus_tag="ACHXOL_002461"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR6809420.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2088..2600)
                     /gene="moaB"
                     /locus_tag="ACHXOL_002462"
     CDS             complement(2088..2600)
                     /gene="moaB"
                     /locus_tag="ACHXOL_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR6809421.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2622..3611)
                     /gene="moaA"
                     /locus_tag="ACHXOL_002463"
     CDS             complement(2622..3611)
                     /gene="moaA"
                     /locus_tag="ACHXOL_002463"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR6809422.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3599..3743)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4008..4916
                     /gene="yvcK"
                     /locus_tag="ACHXOL_002464"
     CDS             4008..4916
                     /gene="yvcK"
                     /locus_tag="ACHXOL_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR6809423.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5108..7129)
                     /gene="uvrB"
                     /locus_tag="ACHXOL_002465"
     CDS             complement(5108..7129)
                     /gene="uvrB"
                     /locus_tag="ACHXOL_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR6809424.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7708..8376)
                     /gene="bioD"
                     /locus_tag="ACHXOL_002466"
     CDS             complement(7708..8376)
                     /gene="bioD"
                     /locus_tag="ACHXOL_002466"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR6809425.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENAATGKYIN
                     LALL"
     gene            complement(8369..9124)
                     /gene="bioC"
                     /locus_tag="ACHXOL_002467"
     CDS             complement(8369..9124)
                     /gene="bioC"
                     /locus_tag="ACHXOL_002467"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR6809426.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9111..10265)
                     /gene="bioF"
                     /locus_tag="ACHXOL_002468"
     CDS             complement(9111..10265)
                     /gene="bioF"
                     /locus_tag="ACHXOL_002468"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR6809427.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10262..11302)
                     /gene="bioB"
                     /locus_tag="ACHXOL_002469"
     CDS             complement(10262..11302)
                     /gene="bioB"
                     /locus_tag="ACHXOL_002469"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR6809428.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11389..12678
                     /gene="bioA"
                     /locus_tag="ACHXOL_002470"
     CDS             11389..12678
                     /gene="bioA"
                     /locus_tag="ACHXOL_002470"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR6809429.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12737..13213
                     /locus_tag="ACHXOL_002471"
     CDS             12737..13213
                     /locus_tag="ACHXOL_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR6809430.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
BASE COUNT         3057 a         3641 c         3433 g         3189 t
ORIGIN      
        1 ttaagtgtcg tttttagtaa gaaaaaaatc ccgacacatg gccgggatag tgatggtgaa
       61 aaagaattaa cggcggttgc cgaagatccg taacaacatc aggaacaggt tgatgaagtc
      121 cagatacaag gttaacgcgc caagaatgga atatttgcgc aggttcgacg tgtcgcgggt
      181 atcaatctgc tcacccatat ttttcaattt ctgagtgtca tacgccgtca atccgacaaa
      241 gacaatcacg ccgatgtagg taactgccca catcaatgct tcgcttttca gccagaagtt
      301 gaccagcgat gccagcacaa tgccgattaa cgccataaac agcatattgc caaagccact
      361 taaatcgcgc ttcgtggtgt aaccgtacag gctcattgcg ccgaacatcc cggcagtaac
      421 gacgaaagta ctggcgatag aagcagcggt atagacaatg aatatactgg aaagcgtaag
      481 acccgtcagc gccgaataaa gcataaagag catcgtcgtt acacctgcgc tcagcttttg
      541 aatcatcgct gataacacaa taaccaatgc taattgcgcg atgatcagac cgattaaaaa
      601 gacacggtta gtgaacaaca gctccatcac ggccgcggaa ttagccgcat accaggcaac
      661 aaatgcggtc agcaacaagc caacggtcat ccagccatag acttgcgcca tataagtttg
      721 caagccagcc cggggttgta cgattgaatc agaacgtggg aatctgtcca tgacgatctc
      781 ctgaagatat aaggaatatc ttaaggatac tgcaaaatga tgaggctgtg catcgacgca
      841 gcgtaaacgc atgtactgag cggtgaaatt gccggacgca gcggtgcctt atccggctaa
      901 caaaaaacta ccagcgtttt gccgcctgct gatcgctctc ccgggcttca acccagcggt
      961 cgccttccgg cgtggcttcg cgcttccaga acggtgcgcg ggttttgaga taatccataa
     1021 taaactgccc ggcttcaaac gcactgctgc gatgcgcact ggtgacaccg acaaaaacga
     1081 tttcatcgcc cggccataat tccccgatgc ggtgaatcac cgtgacgcgc cccagcggcc
     1141 agcggttacg cgcttcatca acgatttctg ccagtgcttt ttcagtcatc cccggatagt
     1201 gttcgagggt taatgctttg acgctgtcac ccaggttatg gttgcgcacc ttaccggtaa
     1261 acgtgaccac tgcaccgtct tcgtcacgct cagccagcca cgggtactct tctcctacgc
     1321 tgaacggctg cgggccgaca acaattttgg tttctgccat cttaacctcc ggttaccggc
     1381 gggaagaaag ctacttcgtc gccgtcagtc agcggatggt caaaactcac cagcgtctgg
     1441 ttgacggcag ccagcaattt gccatcttcc agcgccagcg cccagcgatc gctctgcgca
     1501 gccaggtgct ggcgtaacgc ttcaacggtt gggtaatccg cagccacttc ggttgcatct
     1561 gttcccacca actcgcgcac ctgggcgaaa aaaagaactt taatcatccg cttccacctt
     1621 aaagtcaccc gacttgccgc cgctcttcgc cagcaaacgt accggaccaa tcaccatatc
     1681 tttttgcacc gctttgcaca tgtcataaat ggtcagcgcc gccacggagg ccgcggttaa
     1741 cgcttccatt tcgacaccgg ttttcccggt caggcggcat aaggtttcta tacgcacccg
     1801 attgtgctcc ggctcggcct gtaaattgac ttcaactttg ctgagcatca gcgggtgaca
     1861 cagcgggatc agatcccagg tgcgttttgc cgcctgaata ccgacaatac gcgcagtggc
     1921 aaatacgtcg cctttgtggt ggcgaccatc aataatcatc gccagcgtct cgctgcgcat
     1981 ggtgacaaag gcttccgccc gcgcttcacg cacggtttcc gctttggcgg agacatccac
     2041 catgtgcgct tcgccagcgg cgttgatatg ggtcagttgc gacatactta tttcttcaaa
     2101 tgtggatgga aattacacgg acgcgtacgg gcatccagct gcggcgcgat gatattttcc
     2161 catgcggtac ggcacgcttt ggtcgaaccc ggcatggcga aaatcagcgt tttgttggcg
     2221 acgcccgcta ccgcacgaga ttgcaacgtg gaagtgccaa tctcttcaaa cgacaacatc
     2281 cggaacactt caccaaaacc ttcaacttca cggtcgaaca acggcagcaa tgcttcggga
     2341 gcctgatcac cttccgtcag gccagtcccc cccgtaatca acaccacttg tacatcgtcg
     2401 ctggcgatcc acgcagatac ctgagcgcga atagcgtagc ggttttcttt cacaatggct
     2461 ttatcgacaa cgtgatggcc cgcttcttgc gccgaatcgc gcaaatagtg accggaggta
     2521 tcgtcttctt caccgcgacg attagaaacc gtaagaatag caatacgggt cgggataaat
     2581 tcagtgctta cctgactcat ctgatctctc cttttgacgt tttagccgcc aatgtacgat
     2641 aagttttgcg taataccggt gttgttttga tgcaggaaat gggtctgttt cttctcccgc
     2701 agcgccgctg aaatacgcgc ttccagcgcc tgttgctggg tatcgtcttc cagcagatcg
     2761 cgcaggttaa cgccgccttc accaaacagg cagagatgga gtttaccaat ggaggaaacg
     2821 cgcaggcggt tgcaagtggc gcagaagtct ttttcatacg gcatgataag gccaatctct
     2881 ccggcgtaat ctggatggca aaagacttgc gcgggaccgt cgctgcgttg acgtaattgg
     2941 tggatccagc cgcgacgcag tagctcgtca cgcagaacct gaccagagat gtgatgctta
     3001 cggaagagct cactgccctc gcccgtttcc atcagttcga tgaaacgcag ctggataggg
     3061 cgatgctgga tccagttcag aaaggtgtcg agctggtgat gattaacatc acgcatcagc
     3121 acggtattga ctttgacctt ctcaaaaccg acctcaaatg cagcatcgat ccctgccatg
     3181 acctggttga atttatcctg cccggtaata gcgtgaaact ggcgggcgtc cagactgtcg
     3241 acactgacgt taatgccagt aagtcccgca tcgcgccagt tcgccacatc gcgttccaga
     3301 cggtaaccat tggtggtgac cgcaatctgg cggatagcgt cgttttcccg cacagcggcg
     3361 atgatatcgg taaagtcgcg gcgtaaagac ggctctcctc ctgtcaggcg cactttttcg
     3421 gtgcccagac tggcgaaggc gcgcgtaacc cggcgaattt catcgacggt aagaaagcct
     3481 ttattggtga cgccgctcgg tttgtagcca tccggcaggc agtaggtgca acgaaagtta
     3541 cacacatcgg taatcgacag gcgcaagtag taaaacttac gcgcaaatgc atcagtcagt
     3601 tgtgaagcca tgtacacctt tccagatacg ggaggcgaag tcatttcttc cttcgccctg
     3661 gtggcaatct ttacgagcat tgtcacggcc aaagcaccgt atcagttgac ccaggtgcag
     3721 aggctagagt gtttagtggt taagccgata ctagcgtgta aatgagattt ttaccatcca
     3781 cactttcgct atgtaatcat gtatatagcg tcatgatcgt gctatttcgc tacagaataa
     3841 gggaaaatgg cagttttact ttgatataca tcatcaggga ggcatagcgt gcatcgtcct
     3901 ttagaggtag aagaaaatag tttgtatcct caatattggc aggttaattg ctgtttcccc
     3961 gcaatttgcg ctactgtagc gcggataatc tgactccagg aaactatatg cgcaatcgta
     4021 cgctggctga tcttgatcgt gtcgttgctc tcggcggagg gcatggactg ggacgcgttc
     4081 tctcatcact ttcgtctttg ggttctcgtt taacgggtat cgtcaccacc acggataatg
     4141 gtggctcgac ggggcgtatt cgccgttcag aaggcggcat tgcctggggc gatatgcgca
     4201 actgcctcaa ccaactgata acggaaccga gcgtcgcctc cgcgatgttt gaataccgtt
     4261 ttggcggcaa tggcgaactt tccggtcata atctcggaaa cttgatgtta aaggcgctgg
     4321 atcaccttag cgtgcggcct ctggaagcca tcaatttaat tcgtaatctg ctgaaagtgg
     4381 atacgcattt gattccaatg tcagagcatc ccgtcgatct gatggcgatt gacgatcagg
     4441 ggcatgaagt ttacggcgag gtcaatatcg accagttaac tacgccgatt caagagttat
     4501 tattaacgcc gaatgtaccc gcaacgcgtg aggcggttca cgctatcaat gaagcggatc
     4561 tcatcattat tgggcctggc agtttttata ccagcctgat gccaattctg ctgctgaagg
     4621 aaatcgccca ggcattacgc cgcacgccag cgccgatggt ctatatcggc aatctggggc
     4681 gtgagttgag tttacctgcg gctaatttga agctggaaag caagctggca attatggagc
     4741 agtatgttgg taaaaaggtc attgatgcgg tcatcgtcgg gccaaaagtg gatgtctcgg
     4801 cggtgaaaga gcggattgtg attcaggagg tactggaggc cagcgatatt ccgtatcgtc
     4861 atgaccgcca gttgttacat aacgcgctgg aaaaggcgtt acaggcttta ggttaacgga
     4921 aatccgaaga aaaattccgg cttcctattg aagacaaagt gcgcgttgtt tatgccggat
     4981 gcggcgtgaa cgccttatcc ggcctacaaa ccgcgctaat tcaatgtatt gcagagatca
     5041 tgtaggcctg ataagcgtag cacatcaggc aattttgctt ttgtcatctg tcttcttcgt
     5101 tatcctgtca cgaagccgcg ataaacagct cacgcagctg atgcaactgg tcacgaattt
     5161 gtgccgcttc ttcgaactcc agattctgcg cgtgttgcat cattaaccct tccagctcat
     5221 ggattttctg ttgcaacgct ttaggcgaca tatccatcgg cacattatcc ggctcaacaa
     5281 tcgggcgcga ttttcctctg cccttcgctt tggttttggc aatgttctgc cccagcgcca
     5341 ggatatcgac cactttcttg ttcaagcctt gcggcgtaat tccgtgttcc tcgttgtact
     5401 tctgctgttt ctcacggcga cgttcggttt cgccaatcgc tttcgccatt gatggggtga
     5461 tcttatcgcc gtagagaatc gctttaccgt taacgttacg tgccgcacga ccaatagtct
     5521 ggatcaacga acgttcggaa cgcaggaagc cttctttgtc agcgtcgagg atcgccacca
     5581 gcgacacttc cggcatatcc agaccttcgc gcagtaagtt gatccctacc agcacgtcga
     5641 actcacccag acgcaagtcg cggataatct ccatacgttc gacggtgtcg atatctgaat
     5701 gaagataacg cacgcgctcg ccatgttctt cgagatattc ggtaagatct tccgccatcc
     5761 gcttggtcag tgtggtgacc agtacgcgtt cgttaattgc cgctcgctga cgaatctccg
     5821 aaagaagatc atcaacctgt gtcgccaccg gccgcacttc gataatcggg tcaagcaatc
     5881 cggttggacg caccacctga tccaccacat cgccgccgga tttttccagc tcgtaattac
     5941 ccggcgtcgc cgaaacatag atggtttgcg gcgctaatgc ttcgaactct tcaaacttaa
     6001 gcggacggtt atccagcgct gatggcaggc ggaagccgta ctccaccagt gtctctttac
     6061 gcgcccggtc accgcgatac atgccgccaa tttgtggaat ggtgacgtga gattcatcga
     6121 ccaccagcag cccatcggca ggcaggtaat caaacagcgt cggcggtggc tcacccggtc
     6181 cacgaccgga gaggaagcgc gagtagtttt caatccccga acagtagccc agctcgttca
     6241 tcatctccag atcaaactgg gtacgctggg tcagccgctg ctcttccagc agtttgttgt
     6301 tttccaacag cactttgcgt ctggcggcca gttcttcttt gatctcctcc atcgcctgta
     6361 cgatgcgctc gcgcggtgtg acgtagtgcg ttttcgggta gatggtaaaa cgtggaatag
     6421 tggaaacaat ctgcccggtc agcgggtcaa ataacgacaa tcgttccact tcctcgtcaa
     6481 acagttccac gcgaagtgca atgtcatccg attctgccgg gaagatatct atcacctcgc
     6541 cacgaacgcg gaaagtacca cgctggaatg cttgatcatt acgagcgtat tgcagctccg
     6601 ccagtcggcg cagaatcgcg cgctgatcga taatcatacc gaccgtgaga tggagcatca
     6661 tcttgagata taaatcagga tcgcccagac cataaatcgc ggaaacagag gccactacaa
     6721 ccacatcacg ccgctccagc atcgctttgg tggcggataa acgcatctgc tcaatatgtt
     6781 cgttaaccga ggcatctttc tcaatgaaag tgtcggaact cggtacatag gcttccggct
     6841 gatagtagtc gtagtaggag acgaaatact ccaccgcgtt ttccgggaag aactctttca
     6901 tttcgccata cagctgggcc gccagcgttt tgttgggcgc aagtaccatg gttgggcgct
     6961 gaaggtcagc aatgacattg gcaatggtga aggttttccc tgagccagtc acgccaagta
     7021 acgtctggtg cgccaggcca tcttccagcc cctcttcgag acgtcgaatc gcctctggct
     7081 gatcgccaga aggtttaaaa gcggaattca gtttgaacgg tttactcatg agtcgctacc
     7141 tgaaggagtt gggcgggcag gtatgtaatt ttactcgtcg tacttaatta tgccaacaaa
     7201 ttatactgga taaaaaaaca gttcatcacc ataatatttc tgatacagcg taaactccgc
     7261 tgtcaatcat gagcaaaatt tactctgtgg cgagataaaa ctccggcctt accgggttat
     7321 ccccaaagca acggcttttt taacatttgt caagatgagt gatgacagtt ttatggcaag
     7381 agatgcctgt tcagtgactg ccattgcatt tatctaacca gttaaaaaat aaaagatatt
     7441 tctttgagcc gtctttaacg ccaattcgcg tgcaagccgc gtattctctc gcttgcctcg
     7501 tgttttctaa ctctaataca catggttatc cacaggaata gtggataact gtctccagcc
     7561 cctatccccc gccgcctggg caattcccac attcgccgca tgaggcggaa gtaaaatttt
     7621 tttcgtgatt attttagaat ttaattggtt aaattgcagt caatcgaaga cgcgatctcg
     7681 ctcgcaattt aaccaaatac agaatggcta caacaaggca aggtttatgt actttccggt
     7741 tgccgcattt tctgcaagcc aggggatctc tcccagcagc ggcgcgggaa tcatgcgggt
     7801 gagcgtggtc atatattcag cgtgacgttt tcccggaggc gtaacatcgt tcgccaccca
     7861 acccgccaga gtcagtccgg cgtgttgtat tgcctgcgca gtcaacatcg cgtgattaat
     7921 acagccgagt ttcacaccaa ctaccagtat caccggcagt tgttcctgtg ttacccaatc
     7981 tgcaaaagtg aaagtgtcag aaagcggcgt aaaccagccg ccagcacctt ccactaacac
     8041 ccagtcagcc tgttgttcaa gcgcgcgtaa tccggcgctc attaccaatg attctatcgg
     8101 cctgccctct tgcgcgctga tgatgtgcgg cgaagtgggt tctgcgaagg tgtaaggatt
     8161 tactgttgcg taatccagct gcaggctgct gttgtgctgt aacgccagcg cgtcactatt
     8221 acgtaagcct tccggggtct tttcgctgcc agaggcgacc ggtttataac ctgccgtccg
     8281 ataacctact gccttcgcgg cttgtaaaag tgcacaactg gcgacggttt tccccacttc
     8341 ggtatccgtt ccggtgacaa aataacgttt actcacgagc aatcactccc aaaaaaagat
     8401 gatacgtcag aggatatcgc ccctgttgtt gcggccaggc cagttgcaat cgctgcaatt
     8461 gcgaacgcgt taatattcgc gggtcgcgcc cttcatgaag atgcgtagca ccgatgcctt
     8521 tcagcgaacg catggcactg agcgcatcat caaaccatag tgtgatgggc tgaatatgat
     8581 gttgataatg cacgccgttc agcgactgtt cgatttcatc tggcggtaaa aagcgattag
     8641 catgcggacg ctcgtccacc gcctgccacg cctgatgcag ttcgggtaac gatccctgca
     8701 ccagcgtggt aaacgccacc acgcctccgg ggcgcaccac ccgatacagc tcgcggagtg
     8761 ccgtggataa attaccgcac cactgcaccg cgagattgct ccatgcaaga tcgaacgtcg
     8821 cagtcgctaa cggcagggat tcgatatctc ccgccagata atggtctgca gcatctttct
     8881 ggcgggcttg cacaagcatt ggcggtgaga gatctaacgc cgtcacctgt gcgtgacgct
     8941 ctcgccagtg gcggctcatc cagcctggcc cacaccccgc gtccagtacg tgggtgtatt
     9001 tacgctgtgg gagcattgcc agtaagacgt cagcactccg gcgctgtaac tctgcgtgtt
     9061 gctcatagtg cgcggctgcc cgaccaaatg ccgctgcaat ggcttgttta ttaaccgttg
     9121 ccatgcagca cctccagcag acggtcgata tcctgcattt catgcgctgc ggttagcgtt
     9181 aagcgcagtc gcgcagtacc agcgggtacg gttggcgggc gaatcgccgt gacccagcag
     9241 ccttgctggc gcagtttttc tgccagttgt aacgcacggc tgttatcacc gacgatcaat
     9301 ggctggatgg cgctgcatga atcagcaagc gtaaacggca aatcctgtac tccggcacga
     9361 aaacgcgcaa tgagtgacac cagtttttcg cgccgtgcat ccccctcatc acggcgaatg
     9421 accgccagcg acgcacgtaa tgcctgcgcc tgagcgggcg gcatactggt gctgtagata
     9481 aggtggcggg cgaattgcag cagataatcc gccaccgtac tggagcaaag caccgcagcc
     9541 ccactgacgc caaatccttt gccaaaagtc accaccagca attctggttt taccttttgc
     9601 agccagcagc tgccgcgccc ctgctccccg ataacgcccg tgccgtgggc atcatcgacc
     9661 atcaaccagc cattgtgctg ttgcgttacc tgctggattt ccgccagtgg cgcgctatcg
     9721 ccgtccatgc tgaacacgcc ttctgtcacc accagttgct gccccggaca gggggaagca
     9781 agcagtcgcg ccagatgagc gacatcgtta tgagtaaaac ggcgaagctg cgacgggctt
     9841 aaactggcag cctccagcaa ggaggcatgg ctaagccggt cggcaacaat acggtcctct
     9901 ttcgccatca tcgcggcaat gactgcctga ttcgcggcaa aaccagagat aaacagcagc
     9961 gcccgcgaat agccaagcca ctcggccagt tcttcttcca gcgcctgatg caccacgcta
    10021 taaccgctga catgcccgga accgccgcta cctacaccaa acttttctgc gccctgctgc
    10081 caggcgcgga taatttgcgg atgatggctt aaacctaaat aatcgttact ggaaaagttc
    10141 agatactggc gatcatccgc caccagccag cgtccggctc cttgcgccac cggataacgg
    10201 cgacgcaggg catcggcagt acgccgtgca tcgagcgccg cgttgatttt ctcctgccag
    10261 ctcataatgc tgccgcgttg taatattcgt cggtgtccgg ggtcatcagc gcctgttcaa
    10321 gacgttgctg ttgttcgtta tcccctgcca gcacggcagt ttgctgcgga tttagcccca
    10381 gtttgcggaa cagttgcagg tctttatctt cttccggatt cggcgtggtc agcagtttgc
    10441 aaccgtagaa aatcgagttt gcgccagcca taaagcacat cgcctgagtc tgttcattca
    10501 tctgctcgcg tccggcagaa aggcgcacgt aagaggtcgg catcatgatc cgcgctaccg
    10561 caatagtgcg aataaaatca aaggcatcga catcatcgtt atccgccagt ggcgtgcctt
    10621 tcaccttcac cagcatgttg attggtacgc tttccggcgg cgtcggcagg tttgccagtt
    10681 gcagcaataa tccggcgcga tcttttaccg tttcgcctaa gccaacaatg ccgccagaac
    10741 agactttgat cccggcatcg cgcacttttt ccagcgtatc gaggcgttcc tgataagtgc
    10801 gggtggtgat gatattgccg taaaactccg gcgaggtgtc caggttgtgg ttgtaataat
    10861 ccagcccggc gttcgcgagg cgctgcgcct gagattcact caacgtgccc agcgtcatac
    10921 acgcctccag ccccatcgct tttaccccct gcaccatttg ttccaggtac ggcatatcgc
    10981 gttcgtgggg attcttccac gccgcgccca tacagaagcg cgtcgatcct gccgctttcg
    11041 ctttgcgcgc cgactccagc acctgttcaa cttccatcaa ccgctcggct tccagcccgg
    11101 ttttgtagcg tgagctttgc gggcagtatt tgcaatcttc cggacaagct ccggtcttaa
    11161 tcgacagcaa cgtgctgacc tggacctgac gaggatcgaa atgctggcga tggacctgct
    11221 gcgcttcaaa cagcagatcc agcaacggtt tttcaaataa ttctgtgact tgcgacaagg
    11281 tccagcgtgg gcggtgagcc atggggcttc tccaaaacgt gttttttgtt gttaattcgg
    11341 tgtagacttg taaacctaaa tcttttcaat ttggtttaca agtcgattat gacaacggac
    11401 gatcttgcct ttgaccaacg ccatatctgg cacccataca catccatgac ctccccgctg
    11461 ccggtttatc cggtggtgag cgccgaaggt tgcgagctga ttttgtctga cggcagacgc
    11521 ctggttgacg gtatgtcgtc ctggtgggcg gcgatccacg gctacaatca cccgcagctt
    11581 aatgcggcga tgaagtcgca aattgatgcc atgtcgcatg tgatgtttgg cggtatcacc
    11641 catgcgccag ccattgagct gtgccgcaaa ctggtggcga tgacgccgca accgctggag
    11701 tgcgtttttc tcgcggactc cggttccgta gcggtggaag tggcgatgaa aatggcgttg
    11761 cagtactggc aagccaaagg cgaagcgcgc cagcgttttc tgaccttccg caatggttat
    11821 catggcgata cctttggcgc gatgtcggtg tgcgatccgg acaactcaat gcacagcctg
    11881 tggaaaggct atctgccaga aaacttgttt gctcccgccc cacaaagccg tatggatggc
    11941 gaatgggatg agcgcgatat ggtgggcttt gcccgcctga tggcggcgca tcgtcatgaa
    12001 atcgcggcgg tgatcattga gccgattgtc cagggcgcag gcgggatgcg catgtaccat
    12061 ccggaatggt taaaacgaat ccgcaaaata tgcgatcgcg aaggtatctt gctgattgcc
    12121 gacgagatcg ccactggatt tggtcgtacc gggaaactgt ttgcctgtga acatgcagaa
    12181 atcgcgccgg acattttgtg cctcggtaaa gccttaaccg gcggcacaat gaccctttcc
    12241 gccacactca ccacgcgcga ggttgcagaa accatcagta acggtgaagc cggttgcttt
    12301 atgcatgggc caacttttat gggcaatccg ctggcctgcg cggcagcaaa cgccagcctg
    12361 gcgattctcg aatctggcga ctggcagcaa caggtggcgg atattgaagt acagctgcgc
    12421 gagcaacttg cccccgcccg tgatgccgaa atggttgccg atgtgcgcgt actgggggcc
    12481 attggcgtgg tcgaaaccac tcatccggtg aatatggcgg cgctgcaaaa attctttgtc
    12541 gaacagggtg tctggatccg gccttttggc aaactgattt acctgatgcc gccctatatt
    12601 attctcccgc aacagttgca gcgtctgacc gcagcggtta accgcgcggt acaggatgaa
    12661 acattttttt gccaataacg agaagtccgc gtgagggttt ctggctacac tttctgcaaa
    12721 caagaaagga gggttcatga aactcatcag taacgatctg cgcgatggcg ataaattgcc
    12781 gcatcgtcat gtctttaacg gcatgggtta cgatggcgat aatatttcac cgcatctggc
    12841 gtgggatgat gttcctgcgg gaacgaaaag ttttgttgtc acctgctacg acccggatgc
    12901 gccaaccggc tccggctggt ggcactgggt agttgttaac ttacccgctg atacccgcgt
    12961 attaccgcaa gggtttggct ctggtctggt agcaatgcca gacggcgttt tgcagacgcg
    13021 taccgacttt ggtaaaaccg ggtacgatgg cgcagcaccg ccgaaaggcg aaactcatcg
    13081 ctacattttt accgttcacg cgctggatat agaacgtatt gatgtcgatg aaggtgccag
    13141 cggcgcgatg gtcgggttta acgttcattt ccactctctg gcaagcgcct cgattactgc
    13201 gatgtttagt taatcactct gccagatggc gcaatgccat ctggtatcac ttaaaggtat
    13261 taaaaacaac tttttgtctt tttaccttcc cgtttcgctc aagttagtat aaaaagctga
//
LOCUS       DAZRUF010000109        13261 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZRUF010000109 DAZRUF010000000
VERSION     DAZRUF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.109"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHXOL_002472"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHXOL_002472"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR6809431.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHXOL_002473"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHXOL_002473"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR6809432.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHXOL_002474"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHXOL_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR6809433.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHXOL_002475"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHXOL_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR6809434.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHXOL_002476"
     CDS             complement(4036..5568)
                     /locus_tag="ACHXOL_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR6809435.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHXOL_002477"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHXOL_002477"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR6809436.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHXOL_002478"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHXOL_002478"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR6809437.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="ACHXOL_002479"
     CDS             complement(8663..10399)
                     /locus_tag="ACHXOL_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR6809438.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHXOL_002480"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHXOL_002480"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR6809439.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="ACHXOL_002481"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="ACHXOL_002481"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR6809440.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="ACHXOL_002482"
     CDS             complement(12121..12855)
                     /locus_tag="ACHXOL_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR6809441.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13261
                     /locus_tag="ACHXOL_002483"
     CDS             13056..>13261
                     /locus_tag="ACHXOL_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR6809442.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVV"
BASE COUNT         3367 a         3372 c         3487 g         3035 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgagg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatccggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
    13261 t
//
LOCUS       DAZRUF010000110        13204 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZRUF010000110 DAZRUF010000000
VERSION     DAZRUF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13204
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.110"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(473..1564)
                     /gene="ychF"
                     /locus_tag="ACHXOL_002484"
     CDS             complement(473..1564)
                     /gene="ychF"
                     /locus_tag="ACHXOL_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR6809443.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1681..2265)
                     /gene="pth"
                     /locus_tag="ACHXOL_002485"
     CDS             complement(1681..2265)
                     /gene="pth"
                     /locus_tag="ACHXOL_002485"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR6809444.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2542..2820
                     /gene="ychH"
                     /locus_tag="ACHXOL_002486"
     CDS             2542..2820
                     /gene="ychH"
                     /locus_tag="ACHXOL_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR6809445.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2875..4554)
                     /gene="dauA"
                     /locus_tag="ACHXOL_002487"
     CDS             complement(2875..4554)
                     /gene="dauA"
                     /locus_tag="ACHXOL_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR6809446.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKTNSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4678..5625)
                     /gene="prs"
                     /locus_tag="ACHXOL_002488"
     CDS             complement(4678..5625)
                     /gene="prs"
                     /locus_tag="ACHXOL_002488"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR6809447.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5776..6627)
                     /gene="ispE"
                     /locus_tag="ACHXOL_002489"
     CDS             complement(5776..6627)
                     /gene="ispE"
                     /locus_tag="ACHXOL_002489"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR6809448.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6627..7250)
                     /gene="lolB"
                     /locus_tag="ACHXOL_002490"
     CDS             complement(6627..7250)
                     /gene="lolB"
                     /locus_tag="ACHXOL_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR6809449.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7464..8720
                     /gene="hemA"
                     /locus_tag="ACHXOL_002491"
     CDS             7464..8720
                     /gene="hemA"
                     /locus_tag="ACHXOL_002491"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR6809450.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8762..9844
                     /gene="prfA"
                     /locus_tag="ACHXOL_002492"
     CDS             8762..9844
                     /gene="prfA"
                     /locus_tag="ACHXOL_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR6809451.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9844..10677
                     /gene="prmC"
                     /locus_tag="ACHXOL_002493"
     CDS             9844..10677
                     /gene="prmC"
                     /locus_tag="ACHXOL_002493"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR6809452.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10674..11066
                     /gene="sirB2"
                     /locus_tag="ACHXOL_002494"
     CDS             10674..11066
                     /gene="sirB2"
                     /locus_tag="ACHXOL_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR6809453.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11070..11879
                     /gene="sirB1"
                     /locus_tag="ACHXOL_002495"
     CDS             11070..11879
                     /gene="sirB1"
                     /locus_tag="ACHXOL_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR6809454.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11915..12769
                     /gene="kdsA"
                     /locus_tag="ACHXOL_002496"
     CDS             11915..12769
                     /gene="kdsA"
                     /locus_tag="ACHXOL_002496"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR6809455.1"
                     /translation="MKQKVVSIGDINVANDLSFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12918..13025)
                     /locus_tag="ACHXOL_002497"
     CDS             complement(12918..13025)
                     /locus_tag="ACHXOL_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR6809456.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3285 a         3323 c         3417 g         3179 t
ORIGIN      
        1 tgcgccattt ttgcattact aataagaaaa agcaaaaaac atcgatattc atttttggtc
       61 aatgggtttt ctggcctatc gttgttattg tgctactggt tatcattttc atcttgccag
      121 gatcgcacgt aatcacgtta ttcggggact ttatctgcgg gatattaccc ttcaggtaag
      181 tccttttctc gccagcatat tggagcgtga tcaatattga tcagctgtga agggggtgat
      241 gtgtagttga acaaaaactt acgttatatc actactaatg agatatccgt catctgacac
      301 tgatgtcttt aattattctc gtggttcact gtaggcaata agcacaaaag tgtaggatgt
      361 tacaagaatg attaggactc ggtgaaatga aaaatccacg caattgcgtg gattttatat
      421 acttttgcgc tcttcatgag atttagcgaa acctcatgag acaataaatt aattagacgt
      481 tgaaaaggaa gttcatcaca tcgccatctt taacgatgta atctttacct tctgcacgca
      541 ttttgcctgc ttctttcgcg ccttgttcac ctttgtaagt gatgaaatct tcaaacgaga
      601 tggtttgtgc acggataaag cctttttcaa aatcagtatg gattttgccc gctgcctgcg
      661 gcgcggttgc tccaaccgga atggtccatg cacgcacttc tttcacccca gcggtgaagt
      721 aagtttgcag gttcagcagc ttataaccga cacggatcac acggttcagg cccggttctt
      781 ccagcccaag ctcctgcata aactcgtcac gttcttcgtc gtccagttcg gcaatgtctg
      841 cttcaacagc agcacaaacc ggaaccacaa cagaaccttc tttcgccgcg atttcacgca
      901 cctggtcaag atatgggttg ttttcaaaac cgtcttcgtt gacgttggcg atgtacattg
      961 ttggtttcag cgtcaggaag ctcaggtaac gaatagccgc tttctcttca gcgcttaaat
     1021 ccagcgcgcg cagcatacct gcgttttcca gctggggcag gcatttttct aggaccgcca
     1081 gctcagcttt cgcgtcttta tcgccacctt tggctttctt ctgtacgcga tgaatcgcac
     1141 gttcgcaggt gtcgaggtct gccagcgcca gttcggtgtt gataacttca atatcgtcag
     1201 ccgggttaac tttgccggaa acgtgaatga tgttgtcatt ttcaaagcag cgaacaacgt
     1261 gaccgatcgc ttcggtttca cggatgttgg tcaggaactg gttacccaga ccttcgcctt
     1321 tcgatgcgcc ttttaccaga ccggcgatat cgacaaattc catggtcgtg ggaagcgtac
     1381 gttgcggttt tacgatttca gccagttgat ccaggcgagg gtcaggcatt ggtacgacgc
     1441 ctgtgttcgg ctcaatggtg cagaatggaa agttggccgc ttcaataccg gctttggtca
     1501 gcgcgttgaa cagggtagat ttcccgacgt tgggcaaacc gacgataccg catttgaatc
     1561 ccatgattta aatcacctta atatcttaat aatcaatctg ttattgctaa cagattgcag
     1621 aaatggaaat aactttgcct attatacacg gcactcggca aaaatgccgc agacaacgac
     1681 ttattgcgct ttaaaggcgt gcaatcggtt cgttgctttg gtcaagccat ctgtaaacca
     1741 catttcagta caacgcgccg cttcgtcgat ggcttcatca attaacttct gttcactaac
     1801 aggcggtttg cctaacacaa aaccgacaac tttattttta tcgccaggat gaccgattcc
     1861 gatgcgtaaa cggtgaaagt tagggttatt acccaattta ctgatgatgt ctttcagtcc
     1921 attgtgacca ccatggccac cgcccaattt aaatttggcg acgccaggag gcagatccag
     1981 ttcgtcgtgg gccaccagaa tttcgtccgg attaatgcgg aaaaaactgg ccatcgccgc
     2041 aacggctttg ccgctgagat tcataaatgt agtcgggact aacaggcgga catcttcgcc
     2101 tccaagagtg actcgcgaag tataaccaaa gaatttagcc tcttcgcgca gcggagcgcg
     2161 caaacgctct gccagtaagt caacgaacca ggcaccagca ttatgtcgcg ttgcggcgta
     2221 ttcagcaccg gggttcgcca ggccgacaat caatttaatc gtcacgtttt ttgtcctgag
     2281 tgtgtacata actggcgcgt agtttactgg ttgcggcccc gcttgacaaa aaactgcgta
     2341 tcaaatgcag ataacgtaat aattgcctga gtggactatt agaaagtcaa ggtgttcagg
     2401 cgtttatttg taaagttttg ttgaaataag ggttgtaatt gtgatcacgc ccgcacataa
     2461 cccacagggt gttgtctata ctttacacat aaggaagagg ggtattccct gttacaaccc
     2521 agaaagttcc ggaggtgaca tatgaaacgc aaaaacgctt cgttactcgg taacgtgctc
     2581 atggggttgg ggctggtggt aatggtggtc ggcgtggggt attcaatcct caaccagtta
     2641 ccacagttta atatgcccca gtatttcgca catggtgcag tgctaagtat tttcgtcggt
     2701 gccattctct ggctggcggg tgcccgtgtt ggcgggcatg aacaggtgtg cgaccgttac
     2761 tggtgggttc gtcactatga caaacgttgc cgccgtagcg ataatcgccg tcatagctaa
     2821 caaataatgc agtttgctga ccagtcagtt tgcgctgact ggtcaatctc gtacttataa
     2881 atccgccatc gccgcgcgac gattcgggaa gaacgcgagg cgtcccggga tcggttgaat
     2941 gcctgcgcga gccatagtgc gcagtggctg gaattccacg ttgcacacgc gcagttcaca
     3001 tccttcgggc agacgcttca caaaacgctg gaacgcatca agaccaccag catcaagtac
     3061 cggaacggca tcccacttca gaatcacaat ccgtttgcct tcaagacgtg actccaggtc
     3121 cgtgaacaag ccttcagcag cagcaaaaaa cagcgggcca ataacgcgca gtaccagaac
     3181 atcgtctgga acatctacga ctaccggtgc caggcgagtc atacgtgcga tacgacgcat
     3241 aaacagcagc gatgccagca cgatccccac gctgatggca ataaccatat caaacagcac
     3301 ggtcagcgac atgcacagca gcatgacaat gatgtcatct ttcggtgcat gacgcagcaa
     3361 gtcgaccact ttatgcgctt cactcatgtt ccacgccacc atcaacagca gggctgccat
     3421 agcggaaagc ggcagccagg agagcagcgg tgccagtacc agcagggcaa gaataaccag
     3481 aatagagtgg atcaccgccg agatagggga agttgccccg gcacggacgt tagcggcaga
     3541 acgcgcgatg gcagctgtag cggtaatacc accaaagaac ggagcgataa tattccccag
     3601 tccctgtcca accagttcgc tgttcgtctt gtgtttcgtc ccggtcatac catccagtac
     3661 cacggcgcag agcagagatt cgattgcgcc gagcattgcc attgagaatg ccgcaggcag
     3721 cagtgtgcga atagaatccc aggttagcgt gaattctgaa ttaggcagat cccacggcag
     3781 caccagttgc ggcagcagtt gcggaatacc gttaccctga gaaccatcgg ccaggacgta
     3841 gtggaattgc gaaccgatgg tagcaacatg tccgccgagc aggttaacaa tccccatcac
     3901 cgcgcaacca gccagcaagg ccggaaggtg accgggtaaa cgaatgccca gacgcggcca
     3961 aaaaacaaga atacctagcg tcacaatgcc aatggcagca tcacccacat taatggtcgg
     4021 cagcgccata aataatgcgc cgactttttg tagataatgt tccgggacat gggccatttg
     4081 cagaccgaga aaatctttaa tctgcatggt accgatggtg atcccgatac ccgaggtgaa
     4141 acctaaggtg acggaaaccg gaatatactc aatcaggcga ccaaagcgtg ccagacccat
     4201 cagaatcaaa aagatccccg acagcaaggt cgcaaccagc agtcctgcca gtccaaactg
     4261 ttgcgacacg ggatagagaa ttaccacaaa tgccgcagtc ggaccggaaa cgctaaagcg
     4321 tgacccaccc gtcagagcaa tgacaatccc cgcaacagct gcggtatata aaccgtactg
     4381 gggtgccaca ccactaccaa tagccaacgc catcgccagc gggatagcaa taatcccgac
     4441 ggttatcccg gcaatcaggt cacgggtaaa ccgtgcggca gtatattttt ctttccagca
     4501 agcgtcgatc agagcgcgga aaggcatcac atgtgaggaa aatattttgt tcacaataat
     4561 gtttcatccg tgagcgcatc atctgtcaac taaatggcag gtgaaggagg cataggtcat
     4621 acaaatggat attacagaca aaaaaacccg ccgcagcggg tctttgagcg ggttcgatta
     4681 gtgttcgaac atggcagaga tcgattcttc gttgctgata cgacgaatcg cttcggccag
     4741 catacctgac agggtcagag tacgcacgtt cggcagtgat ttgatttcat cgctcagcgg
     4801 aatggtatcg cagacaacga cttcatcaat tacagagtta cgcaggttgt tcgccgcgtt
     4861 gccagagaag atcgggtgag tcgcgtacgc aaatacacgt ttagcaccac gttctttcag
     4921 agcttcagca gctttacaca gcgtaccgcc agtgtcgatc atatcatcga ccagtacgca
     4981 gtcacgacct gcaacgtcac cgatgatatg catcacctgg gaaacgttcg cacgcggacg
     5041 acgtttgtcg atgattgcca tatcggtatc gttcagcagc ttagcgatag cgcgggcacg
     5101 cacaacgccg ccgatgtccg gagaaaccac aattgggtta tccagattca gctgcagcat
     5161 gtcttccagc aggatcgggc taccaaatac gttatcaacc ggaacgtcga agaaaccctg
     5221 aatctgttca gcgtgcagat ccactgtcag cacacggtca acaccgacgc tggagaggaa
     5281 gtctgcaacc actttcgcag tgattggtac acgagcggaa cggacgcgac ggtcctggcg
     5341 cgcatagcca aagtagggga taacagcggt gatacgacct gcggaagcac gacgcagggc
     5401 atcaaccata acgactaatt ccatcaggtt gtcgttagta ggggcacaag tggactggat
     5461 gatgaaaata tcaccaccgc gtacattttc gttgatttgt acactgactt cgccgtcgct
     5521 aaagcgacct acagcggcgt cgccgagtga agtgtacagg cggttggcaa tacgttgtgc
     5581 tagttccggg gtggcgttac cagcaaaaag cttcatatca ggcacgagaa gaacctcagg
     5641 catgcgtcca ttggtggaaa gaatctgccg aaaactgtgc gggccaggca tgatcctttc
     5701 caggcggtgt attaaagagc gcgatgcaac gtctggaaca aggtgacgtt gtcaccgaaa
     5761 ctcagcttgc ccggcttaaa gcatggctct gtgcaatggg gaaagattaa cacctttcgc
     5821 cacaaagcca ttgagccatt ccggggcttg ctctagcacc tggcgggctt cagactctgt
     5881 atcaaattca gcaaagacac aggcccctgt cccagtcagg cgcgacgggg cgtattctaa
     5941 cagccaggaa agcaccgcat caacctcgcg aaaacgtttt cttgcgataa cctcgcaatc
     6001 attgctgaat tcacatttta gcaacgtttc tattgacctt tttggcgtat tgcgcgggag
     6061 ttcaggatct ttaaaaatca ctggtgtcgg aatacttaca ccagggtgcg ccaccagata
     6121 ccacttctct ggcggatcca ccggcgttag tatttcacca acgccttcgg caaacgcggc
     6181 atgcccccga acaaagacag gaacatctgc gcccagcgtc agccccattt ccgccagctc
     6241 atccatgctt agcccgcatt gccagagatg atttaatgcc accaggaccg tcgcggcatt
     6301 ggatgaacca ccgccgagac cgccgcccat cggcaaacgc ttgtcaatgc tgatattcgc
     6361 accgcttccc gtcggaagac gcccgctgtc tgccgcagtt ttcatcaaca accgcgctgc
     6421 gcgaacgatc aggttatctt catgttccac gccttcaacg ggcgttaaca gacgaatatc
     6481 cccatcgtca cgaagctcaa tgctgatggt gtcgccgtaa tcaagaaact gaaacagcgt
     6541 ttgcagcgtg tggtaaccat ccgcacgctg accggtaatg tataaaaaca gattaagttt
     6601 tgccggagag ggccactgtg tccgcattat ttcactatcc agttatccat ttttaacttg
     6661 atgcgttgac caccgtcggt gagttccata ttggctggca tcgcaggttg cgttttggtg
     6721 tcataaccac cataaacaac cttccagttt ttgccattct ggctgtaggt aatttcgctc
     6781 aggcggtact ggtcgtccag tttgtagtcg gttgcatcac ccggtaaacc taaaatccat
     6841 tggcgcaagc tgttgagcgg aattggcatt ccggtcaatt tgccaatcat ctcttcggcg
     6901 tcatcggcgg tataacgctg acctttattg tcgactaact gcacgttacc cggttgagca
     6961 ttcagctcca gttccgtgct gcccaatggg ttagtgagca gcagacggta gcgatcctgg
     7021 ccggtttgct gccagaaaaa gcgggcgtac actttttgtt ggtcagaaat ataagcgaac
     7081 gcgccgcgag tctgatactg attaagattg cgcacgtctt gctgatgctg acgccattgt
     7141 ggcgaatccg ggcttttgcc aggacctttg ggcgtggtaa cggaacaggc agtgagcaca
     7201 agagcagcca gcggtagcag gcggataaga cgaaaatcgg gcaggggcat agtgatgaca
     7261 agtccttgag atacgttgca gttataaccc ttaatgctag cgttaccgtc cgctatcgtc
     7321 tatgttcaag ttgtcttaat tgccagaatc taacggcttt cggcaattac tccaaaaggg
     7381 ggcgctctct tttattgatc ttacgcatcc tgtatgatgc aagcagacta accatatcaa
     7441 cgttggtatt atttcccgca gacatgaccc ttttagcact cggtatcaac cataaaacgg
     7501 cacctgtatc gctgcgagaa cgtgtatcgt tttcgccgga taagctcgat caggcgcttg
     7561 acagcctgct tgcgcagccg atggtgcagg gcggcgtggt gctgtcgacg tgcaaccgca
     7621 cggaacttta tcttagcgtt gaagagcagg ataacctgca agaggcgtta atccgctggc
     7681 tttgcgatta tcacaatctt aatgaagaag atctgcgtaa aagcctctac tggcatcagg
     7741 ataacgacgc ggttagccat ttaatgcgtg ttgccagcgg cctggattca ctggttcttg
     7801 gggagccgca gatcctcggt caggttaaaa aagcgtttgc cgattcgcaa aaaggtcata
     7861 tgaaggccag cgaactggaa cgcatgttcc agaaatcttt ctctgtagcg aaacgcgttc
     7921 gcactgaaac agatatcggt gccagtgctg tgtctgtcgc ttttgcggct tgtacgctgg
     7981 cgcggcagat ctttgaatcg ctctctacgg tcacagtgtt gctggtaggc gcgggcgaaa
     8041 ccatcgagct ggtagcgcgt catctgcgcg aacataaagt acagaagatg attatcgcca
     8101 accgcactcg cgaacgtgcc caaattctgg cagatgaagt tggcgcggaa gtgattgccc
     8161 tgagtgagat cgacgaacgt ctgcgcgaag ccgatatcat catcagttcc accgccagcc
     8221 cgttaccgat tatcgggaaa ggcatggtgg agcgcgcatt aaaaagtcgt cgtaaccaac
     8281 caatgctgtt ggtggatatt gccgttccgc gcgatgttga gccggaagtt ggcaaactgg
     8341 cgaatgctta tctttatagt gtggacgatc tgcaaagcat catttcgcac aacctggcgc
     8401 agcgtaaagc cgcagcggtt gaggcggaaa ctattgtcgc tcaggaaacc agcgaattta
     8461 tggcgtggct gcgagcacaa agcgccagcg aaaccattcg cgagtatcgc agccaggcag
     8521 agcacgttcg cgatgagtta accgccaaag cgttagcggc ccttgagcag ggcggcgacg
     8581 cgcaagccat tatgcaggat ctggcatgga aactgactaa ccgcttgatc catgcgccaa
     8641 cgaaatcact tcaacaggcc gcccgtgacg gggataacga acgcctgaat attctgcgcg
     8701 acagcctcgg gctggagtag cagtacatca ttttcttttt ttacagggtg catttacgcc
     8761 tatgaagcct tctatcgttg ccaaactgga agccctgcat gaacgccatg aagaagttca
     8821 ggcgttgctg ggtgacgcgc aaactatcgc cgaccaggaa cgttttcgcg cattatcacg
     8881 cgaatatgcg cagttaagtg atgtttcgcg ctgttttacc gactggcaac aggttcagga
     8941 agatatcgaa accgcacaga tgatgctcga tgatcctgaa atgcgtgaga tggcgcagga
     9001 tgaactgcgc gaagctaaag aaaaaagcga gcaactggaa cagcaattac aggttctgtt
     9061 actgccaaaa gatcctgatg acgaacgtaa cgccttcctc gaagtccgag ccggaaccgg
     9121 cggcgacgaa gcggcgctgt tcgcgggcga tctgttccgt atgtacagcc gttatgccga
     9181 agcccgccgc tggcgggtag aaatcatgag cgccagcgag ggtgaacatg gtggttataa
     9241 agagatcatc gccaaaatta gcggtgatgg tgtgtatggt cgtctgaaat ttgaatccgg
     9301 cggtcatcgc gtgcagcgtg ttcctgctac ggaatcgcag ggtcgtattc atacttctgc
     9361 ttgtaccgtt gcggtaatgc cagaactgcc tgacgcagaa ctgccggaca tcaacccagc
     9421 agatttacgc attgatactt tccgctcgtc aggggcgggt ggtcagcacg ttaacaccac
     9481 cgattcggca attcgtatta ctcacttgcc gaccgggatt gttgttgaat gtcaggacga
     9541 acgttcacaa cataaaaaca aagctaaagc actttctgta cttggtgctc gtatccacgc
     9601 tgcagaaatg gcaaaacggc aacaggccga agcgtctacc cgtcgtaacc tgctggggag
     9661 tggcgatcgc agcgaccgta accgtactta caacttcccg caggggcgcg ttaccgatca
     9721 ccgcatcaac ctgacgctct accgtctgga tgaagtgatg gaaggtaagc tggatatgct
     9781 gattgaaccg attatccagg aacatcaggc cgaccaactg gcggcgttgt ccgagcagga
     9841 ataatggaat atcaatactg gttacgtgaa gcaataagcc aacttcaggc gagcgaaagc
     9901 ccacgccgtg acgctgaaat cttattggag tatgtcactg gcagggggcg gacttttatt
     9961 ctggcctttg gtgaaacgca gctgactgac gaacaatgtc agcaacttga tgcgctactg
    10021 acacgtcgtc gcgatggtga acccattgct catttaaccg gggtgcgaga attctggtcg
    10081 ttgccgttat ttgtttcgcc agcgacctta attccgcgcc cggatacgga gtgtctggtg
    10141 gagcaggcac tggcgcggtt gcctgaacaa ccttgccgta ttctcgatct cgggacgggt
    10201 accggggcga ttgcgcttgc gctggctagc gagcgcccgg actgcgaaat tatcgctgta
    10261 gatcgtatgc ctgatgctgt ctccctggca caacgtaatg cccagcatct ggcgatcaaa
    10321 aatatccaca ttctgcaaag cgactggttt agcgcgctag ccgggcagca gtttgcgatg
    10381 attgtcagca atccgccgta tattgacgag caggacccac atcttcaaca aggcgatgtc
    10441 cgctttgagc cgctcactgc gctggttgcg gcagacagtg gaatggcaga catcgtgcat
    10501 atcatcgaac agtcgcgtaa cgcgctggta tccggcggct ttctgcttct ggaacatggc
    10561 tggcagcagg gcgaagcggt gcgacaagca tttatcctcg cggggtatca tgacgtcgaa
    10621 acctgccgtg actatggtga taacgagcgc gtaacgctcg gccgctatta tcaatgacaa
    10681 gtttttctac actgcttagt gttcatctta ttagtatcgc gctttctgtt gggctattaa
    10741 ccttacgttt ctggctacgt tatcagaagc atccacaggc atttgctcgc tggacgcgca
    10801 ttgtgccgcc ggttgtcgat acggtgttat tgttaagcgg cattgcgttg atggctaaag
    10861 cgcacatcct gccattttcc gggcaggcac agtggctgac tgaaaagctg tttggagtta
    10921 tcatttatat cgttttgggt tttattgcac tcgattatcg tcgtatgcac agtcagcagg
    10981 cgcgcattat tgccttcccg ctggcgttgg tggtgctgta catcatcatt aaactcgcca
    11041 ccacaaaagt accgttactg gggtaagtca tgagatcgtt agctgatttc gaatttaata
    11101 aagcgccatt gtgcgaaggc atgatcctgg cttgcgaagc aatccgccgc gattttccct
    11161 cgcaagatgt ttacgacgaa ctggagcgtc tcgttagtct ggcgaaggaa gaaatcagcc
    11221 agcttctgcc tttagaagag cagctggaaa aactgatcgc gctgttttac ggcgactggg
    11281 gatttaaagc ctcacgcggc gtttatcgtc tttccgatgc attatggctg gaccaggtgt
    11341 taaagaatcg acagggcagt gcggtatcat taggtgcggt tttattatgg gtcgcgaatc
    11401 gtctcgattt gccgctgctg ccggtgattt tccctacgca gctgatattg cgcattgaat
    11461 gtccggatgg cgaaatttgg ctgattaatc cttttaacgg tgaatcgtta agcgaacata
    11521 tgctggacgt atggttaaag ggaaatatca gcccgtcggc ggaactgttt tatgaagacc
    11581 ttgatgaagc tgataacatt gaggtaatcc gcaaattgtt ggatacactc aaagcctcgt
    11641 tgatggaaga aaatcagatg gagctggcgt tacgcaccag cgaagcttta ttacaattca
    11701 acccggaaga tccctatgaa attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg
    11761 aacacgttgc gttgaacgat ttaagttatt tcgttgaaca gtgtccggaa gacccgatca
    11821 gcgaaatgat ccgtgcgcaa ataaataaca tcgcgcataa acatattgtg ctgcattaat
    11881 taatcgacat tttactcaag attaaggcga tcctatgaaa caaaaagtgg ttagcattgg
    11941 cgacatcaac gtagcaaatg acctgtcgtt cgtactgttt ggtggtatga acgtgctgga
    12001 atctcgcgat ctggcgatgc gcatttgcga gcactacgta accgtgaccc agaaactggg
    12061 tatcccttac gtgttcaaag cctcttttga caaagccaac cgctcctcca tccactctta
    12121 tcgtggaccg ggcctggaag aagggatgaa aatcttccag gagttgaagc agacttttgg
    12181 cgtgaaaatt atcaccgacg ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt
    12241 ggatgtgatt cagttgccgg cgtttcttgc tcgccagact gacctggttg aagccatggc
    12301 gaaaaccggt gcggtaatta acgtcaagaa accacagttt gtcagcccgg gacagatggg
    12361 taatatcgtt gataaattca aagaaggcgg caacgaaaaa gtgattcttt gcgatcgcgg
    12421 tgctaacttc ggctatgaca acctggttgt cgatatgctg ggcttcagca ttatgaagaa
    12481 agtgtctggt aactcgccgg tgattttcga cgtgacccac gcactgcaat gccgcgatcc
    12541 gtttggcgca gcttccggtg gtcgtcgtgc tcaggtggct gagctggcac gagccggtat
    12601 ggcggtaggt ctggcggggc tgtttattga agcgcatccg gatccggaac atgcgaaatg
    12661 tgatggtcca tccgcgctgc cgctggctaa actggaaccg ttcctcaagc agatgaaagc
    12721 gattgatgat ctggtgaaag gtttcgaaga actggatacc agcaagtaat cttttttgct
    12781 tgaaaaataa agtcttagcg ttctgcgtta agactttttt catgggtgcc agatacaaaa
    12841 aaggccgcag gctgttaccc ctgcggccgg tttcgggcgc atattgccat cacggcagcc
    12901 tgacgcccgt tttcacctta cttccggtta cgccaccagc cgacaatcgc tgcggtaata
    12961 attcccgcca ggatcggtgc tgccaggtcg tgccagaaaa tcatggcaaa ctgcgcgagc
    13021 gtcatatagc cgccttgttg taatgacaac attttgcggc tattcttgaa ttgttctggt
    13081 tcaagattag cccccgttct gttgtcaggt taaacctctc aacgtgcggg ggttttctct
    13141 ttccagcaac caatgccacc agggataaag cccccgcaac attgcgcctc accggataac
    13201 gccg
//
LOCUS       DAZRUF010000111        13143 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZRUF010000111 DAZRUF010000000
VERSION     DAZRUF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.111"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..223)
                     /locus_tag="ACHXOL_002498"
     CDS             complement(<1..223)
                     /locus_tag="ACHXOL_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR6809457.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYESYIANYPEAG"
     gene            complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHXOL_002499"
     CDS             complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHXOL_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR6809458.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHXOL_002500"
     CDS             complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHXOL_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR6809459.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            1466..1594
                     /locus_tag="ACHXOL_002501"
                     /pseudo
     CDS             1466..1594
                     /locus_tag="ACHXOL_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHXOL_002502"
     CDS             complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHXOL_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR6809460.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2149..3807
                     /gene="fliF"
                     /locus_tag="ACHXOL_002503"
     CDS             2149..3807
                     /gene="fliF"
                     /locus_tag="ACHXOL_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR6809461.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3800..4795
                     /gene="fliG"
                     /locus_tag="ACHXOL_002504"
     CDS             3800..4795
                     /gene="fliG"
                     /locus_tag="ACHXOL_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR6809462.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4788..5474
                     /gene="fliH"
                     /locus_tag="ACHXOL_002505"
     CDS             4788..5474
                     /gene="fliH"
                     /locus_tag="ACHXOL_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR6809463.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5474..6847
                     /gene="fliI"
                     /locus_tag="ACHXOL_002506"
     CDS             5474..6847
                     /gene="fliI"
                     /locus_tag="ACHXOL_002506"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR6809464.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHXOL_002507"
     CDS             6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHXOL_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR6809465.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7306..8433
                     /locus_tag="ACHXOL_002508"
     CDS             7306..8433
                     /locus_tag="ACHXOL_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR6809466.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8538..9002
                     /gene="fliL"
                     /locus_tag="ACHXOL_002509"
     CDS             8538..9002
                     /gene="fliL"
                     /locus_tag="ACHXOL_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR6809467.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            9007..10011
                     /gene="fliM"
                     /locus_tag="ACHXOL_002510"
     CDS             9007..10011
                     /gene="fliM"
                     /locus_tag="ACHXOL_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR6809468.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10008..10421
                     /gene="fliN"
                     /locus_tag="ACHXOL_002511"
     CDS             10008..10421
                     /gene="fliN"
                     /locus_tag="ACHXOL_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR6809469.1"
                     /translation="MSDINNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10424..10789
                     /gene="fliO"
                     /locus_tag="ACHXOL_002512"
     CDS             10424..10789
                     /gene="fliO"
                     /locus_tag="ACHXOL_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR6809470.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10789..11526
                     /gene="fliP"
                     /locus_tag="ACHXOL_002513"
     CDS             10789..11526
                     /gene="fliP"
                     /locus_tag="ACHXOL_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR6809471.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHXOL_002514"
     CDS             11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHXOL_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR6809472.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11814..12599
                     /gene="fliR"
                     /locus_tag="ACHXOL_002515"
     CDS             11814..12599
                     /gene="fliR"
                     /locus_tag="ACHXOL_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR6809473.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12884..>13143
                     /gene="rcsA"
                     /locus_tag="ACHXOL_002516"
     CDS             12884..>13143
                     /gene="rcsA"
                     /locus_tag="ACHXOL_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="with RcsB is a positive regulator of capsular
                     polysaccharide synthesis genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR6809474.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIA"
BASE COUNT         3226 a         3398 c         3451 g         3068 t
ORIGIN      
        1 gacctgcttc tgggtaattc gcaatataag attcatatcc acagggaaat aatattatct
       61 gattattatc aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga
      121 tacgacatga agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc
      181 gtatttcttc aatctttagg ttagtcctat cttttgccag catactctta tcctacttta
      241 cctcataaac gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa
      301 ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt
      361 taaactggta atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat
      421 agccaaaatc atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc
      481 ctgcatcagc gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc
      541 atccgctgcc tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt
      601 ctggagcacc agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag
      661 aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa
      721 atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt
      781 tcataaaaac ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt
      841 atgcaaaaag gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat
      901 tagccataat atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg
      961 gtgcgcttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta
     1021 tgatggctgg caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa
     1081 cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct
     1141 gagctaataa ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc
     1201 gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc
     1261 caccaagata aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg
     1321 tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc
     1381 tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa
     1441 aagagttcac tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc
     1501 gtggatggca gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt
     1561 tgccccatcc atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct
     1621 acacctgcat ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc
     1681 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc
     1741 cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta
     1801 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt
     1861 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     1921 gtatcgctga cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca
     1981 ataagacaaa ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa
     2041 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     2101 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     2161 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     2221 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     2281 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     2341 agccaactga cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa
     2401 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     2461 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     2521 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     2581 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     2641 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     2701 gaagggcaaa ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg
     2761 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     2821 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     2881 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     2941 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     3001 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     3061 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     3121 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     3181 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     3241 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     3301 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     3361 gaagatctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     3421 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     3481 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     3541 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     3601 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     3661 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     3721 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     3781 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     3841 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     3901 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta
     3961 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     4021 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     4081 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     4141 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     4201 ggtgcatctg aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     4261 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     4321 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     4381 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     4441 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     4501 cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     4561 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     4621 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     4681 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     4741 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     4801 tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca
     4861 tggtcgagcc ggaagaaacc atcattgaag aggccgaacc tagccttgag cagcaactgg
     4921 cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     4981 agcaaggtca tgagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg
     5041 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     5101 ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc
     5161 tggaggcggc acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca
     5221 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     5281 tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg
     5341 gctggcgctt gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg
     5401 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     5461 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     5521 agccaaaatg gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg
     5581 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     5641 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     5701 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc
     5761 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     5821 attaggtcgc gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac
     5881 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     5941 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca
     6001 gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc
     6061 acgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     6121 aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc
     6181 cgctccggcg gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat
     6241 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     6301 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     6361 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     6421 tagcggcggc ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca
     6481 ggaccccatt gcagactccg cacgggcgat cctcgacggt cacattgtgc tgtctcgccg
     6541 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     6601 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     6661 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     6721 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat
     6781 ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     6841 gtcataaccc aggagataac ggtagatggc agaacatggt gcgctggcga ccctgaaaga
     6901 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     6961 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     7021 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     7081 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     7141 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     7201 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     7261 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     7321 cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     7381 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcaa gcgagacaac taccgacaaa
     7441 gcggcccccc aggtgctggt ggcaacagat aagcccacga caaaaggcga gctgctgatc
     7501 agcgatattg tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacactg
     7561 cctgtcatca acgtcgaaca atccacatcg acaccgttaa ccaccgctca tacgatgacg
     7621 ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa
     7681 gacctcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     7741 aaagtgactg atgcgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     7801 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag
     7861 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     7921 ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc actgctcacc
     7981 gtcgccgcgc ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     8041 cagcatattt cgctgttcac ccgccaggga caacaaagtg cagagttgcg tctgcacccg
     8101 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     8161 atggtttcgc cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     8221 acgcagctgg ccgagagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     8281 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     8341 cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     8401 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     8461 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     8521 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     8581 ggctcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     8641 ggcattcgca tcaggttgcc gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg
     8701 tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata
     8761 tcggtataac cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     8821 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     8881 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
     8941 ttgccgggca accgaaacag gatgtcaccg acgtgatgta taccgctttt attctgcgat
     9001 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
     9061 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
     9121 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
     9181 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
     9241 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
     9301 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt
     9361 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcaatgg acgcttcccg
     9421 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta
     9481 aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag
     9541 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
     9601 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt
     9661 ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
     9721 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
     9781 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
     9841 aaacccggcg acgtcctgcc gatagaaaaa cccgatggca tcatcgccca tgttgacggc
     9901 gtcccggtgc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa
     9961 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacataa
    10021 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
    10081 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
    10141 gtgatgtcag cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga
    10201 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
    10261 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
    10321 taatcgccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
    10381 tcattactcc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac
    10441 tgtgcaatct tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat
    10501 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc
    10561 gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
    10621 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
    10681 aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga
    10741 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg
    10801 ttgtctgtcg cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg
    10861 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
    10921 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
    10981 acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca
    11041 cctaaccagg tattgctggg gctggcacta tttttgacct tttttattat gtcatcggtg
    11101 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
    11161 gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca gacccgtgag
    11221 gcagatttag ggttgtttgc cagactagcg aataccggcc cgttgcaggg acctgaagcc
    11281 gtgccgatgc gcattttgct cccggcctac gtgaccagcg aattgaaaac cgcatttcag
    11341 ataggcttca cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg
    11401 atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg
    11461 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    11521 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    11581 gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc
    11641 agtattttgc aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc
    11701 atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    11761 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc
    11821 aggtgacaag cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg
    11881 tgctggcgct catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa
    11941 aactggggct ggcaatgatg atcacgttcg tcattgcccc atcattacct gccaacgatg
    12001 ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg
    12061 cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg
    12121 gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc
    12181 ccgttttagc gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc
    12241 atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac
    12301 cgttgaacag caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg
    12361 ggctgatgct ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt
    12421 tactgaatcg tatggccccg caattatcca tttttgttat tggatttcca ttaactctga
    12481 ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt
    12541 tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
    12601 tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct
    12661 tccaggatac tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta
    12721 ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa
    12781 aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt
    12841 caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat
    12901 ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa
    12961 aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca
    13021 gcgcccttca gtggtgttta ttaatgagga ctgtttcatc catgatgctt ctaacagtca
    13081 gcacatcaag cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat
    13141 tgc
//
LOCUS       DAZRUF010000112        13023 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZRUF010000112 DAZRUF010000000
VERSION     DAZRUF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.112"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            243..578
                     /locus_tag="ACHXOL_002517"
     CDS             243..578
                     /locus_tag="ACHXOL_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR6809475.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            736..1179
                     /gene="yjdN"
                     /locus_tag="ACHXOL_002518"
                     /pseudo
     CDS             736..1179
                     /gene="yjdN"
                     /locus_tag="ACHXOL_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            1312..2100
                     /gene="phnC"
                     /locus_tag="ACHXOL_002519"
     CDS             1312..2100
                     /gene="phnC"
                     /locus_tag="ACHXOL_002519"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR6809476.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESAGIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2125..3141
                     /gene="phnD"
                     /locus_tag="ACHXOL_002520"
     CDS             2125..3141
                     /gene="phnD"
                     /locus_tag="ACHXOL_002520"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR6809477.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3267..4046
                     /gene="phnE"
                     /locus_tag="ACHXOL_002521"
     CDS             3267..4046
                     /gene="phnE"
                     /locus_tag="ACHXOL_002521"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR6809478.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4067..4792
                     /gene="phnF"
                     /locus_tag="ACHXOL_002522"
     CDS             4067..4792
                     /gene="phnF"
                     /locus_tag="ACHXOL_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR6809479.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4793..5245
                     /gene="phnG"
                     /locus_tag="ACHXOL_002523"
     CDS             4793..5245
                     /gene="phnG"
                     /locus_tag="ACHXOL_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR6809480.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5242..5826
                     /gene="phnH"
                     /locus_tag="ACHXOL_002524"
     CDS             5242..5826
                     /gene="phnH"
                     /locus_tag="ACHXOL_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR6809481.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5826..6890
                     /gene="phnI"
                     /locus_tag="ACHXOL_002525"
     CDS             5826..6890
                     /gene="phnI"
                     /locus_tag="ACHXOL_002525"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR6809482.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6883..7728
                     /gene="phnJ"
                     /locus_tag="ACHXOL_002526"
     CDS             6883..7728
                     /gene="phnJ"
                     /locus_tag="ACHXOL_002526"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR6809483.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7725..8483
                     /gene="phnK"
                     /locus_tag="ACHXOL_002527"
     CDS             7725..8483
                     /gene="phnK"
                     /locus_tag="ACHXOL_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR6809484.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8594..9274
                     /gene="phnL"
                     /locus_tag="ACHXOL_002528"
     CDS             8594..9274
                     /gene="phnL"
                     /locus_tag="ACHXOL_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR6809485.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            9271..10407
                     /gene="phnM"
                     /locus_tag="ACHXOL_002529"
     CDS             9271..10407
                     /gene="phnM"
                     /locus_tag="ACHXOL_002529"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFR6809486.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10407..10964
                     /gene="phnN"
                     /locus_tag="ACHXOL_002530"
     CDS             10407..10964
                     /gene="phnN"
                     /locus_tag="ACHXOL_002530"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR6809487.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10951..11385
                     /gene="phnO"
                     /locus_tag="ACHXOL_002531"
     CDS             10951..11385
                     /gene="phnO"
                     /locus_tag="ACHXOL_002531"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR6809488.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11387..12145
                     /gene="phnP"
                     /locus_tag="ACHXOL_002532"
     CDS             11387..12145
                     /gene="phnP"
                     /locus_tag="ACHXOL_002532"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR6809489.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12292..12621)
                     /gene="yjdP"
                     /locus_tag="ACHXOL_002533"
     CDS             complement(12292..12621)
                     /gene="yjdP"
                     /locus_tag="ACHXOL_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR6809490.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12690..13007)
                     /locus_tag="ACHXOL_002534"
     CDS             complement(12690..13007)
                     /locus_tag="ACHXOL_002534"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR6809491.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         2860 a         3907 c         3666 g         2590 t
ORIGIN      
        1 agaaagataa aaatatcatg tgaataaaaa aaagaacaag tagagcatta acattatctt
       61 aaataataaa tagaggcaaa aagattattt tctttttgcg tttcctttca aatgaaaacg
      121 atcgtcgtct aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg
      181 cctttttcgg caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc
      241 acatgtcatt accacactgc ccaaaatgca actccgaata cacttacgaa gataacggca
      301 tgtacatctg cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg
      361 acgagctgat cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca
      421 tcattaaaga tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga
      481 aaaacatccg cctggttgaa ggcgaccata acatcgattg caaaatcgac ggttttggtc
      541 cgatgaaact gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg
      601 gataaggcgc tcgcgccgca tccggcaacg atgccgactg cctgatgcga cgcttgcgcg
      661 tcttatcagg cagcctctca tctgtataaa tttcgaacta cacttaattg gcttctctta
      721 actgaggtca ccatcatgcc gttaagtccc tacctctctt ttgccggtaa ctgtgccgac
      781 gcaattgctt attatcaacg tacgttgggc gctgaactgc tctataaaat cagcttcggc
      841 gaaatgccaa aatcagcgca ggacagcgcc gagaactgcc cttccggaat gcaatttccc
      901 gataccgcca tcgcccacgc caatgtgcgc attgccggaa gcgacatcat gatgagcgat
      961 gccatgccgt caggaaaagc cagctactcc ggctttacgc tggtgctcga ttcgcaacag
     1021 gtcgaagaag gaaaacgctg gtttgacaat cttgccgcta acggaaaaat cgaaatggcc
     1081 tggcaggaaa ctttctaggc gcatggcttt ggcaaagtca ccgataaatt tggcgtaccg
     1141 tggatgatta atgtcgtcaa acaacaacca acgcaataac ccgccgggag gcccgccctc
     1201 ccgcactgtc atcgaattcc cgttaactct tcatctgtta gtcactttta attaaccaaa
     1261 tcgtcacaat aatccgccac gatggagcca cttttttagg gaggctgcat catgcaaacg
     1321 attatccgtg tcgagaagct cgccaaaacc ttcaatcagc atcaggcgct gcatgcggtt
     1381 gatctgaaca ttcatcacgg tgaaatggtg gctctgcttg ggccgtcggg ttccggcaaa
     1441 tccacccttt tacgtcactt aagcggtttg attaccggcg ataaatccgc cggcagccat
     1501 atcgagctgc tgggccgcac agtccagcgc gaaggccgtc tggcgcgcga tatccgcaaa
     1561 agccgcgcca acaccggcta catcttccaa caattcaacc tggtgaaccg cctgagcgta
     1621 ctggagaacg tgctgattgg cgcactcggc agcacgccgt tctggcgcac ctgttttagc
     1681 tggtttaccc gcgagcagaa acaacgcgcg ttacaggcgc tgacccgcgt tggcatggtg
     1741 cattttgccc atcaacgcgt ttccaccctc tccggcggac agcagcagcg tgtggcgatt
     1801 gcccgcgcgc tgatgcagca ggcgaaggtg attctggccg atgaacccat cgcctcgctg
     1861 gacccggaat ccgccggcat cgtgatggac accctgcgcg acatcaatca gaacgacggc
     1921 atcaccgtgg tcgtcacgct gcatcaggtg gattacgccc tgcgctactg cgaacgcatc
     1981 gtcgccctgc gccaggggca cgttttctac gacggcagca gccaacagtt tgataacgaa
     2041 cgttttgacc atctctaccg cagcattaat cgcatcgaag agaacgcgaa agctgcctga
     2101 catccccatc attgaggaaa acgaatgaac gctaagataa ttgcctcgct ggccttcacc
     2161 agcatgttca gcctcagcac cctgttaagc ccggcacacg ccgaagagca ggaaaaggcg
     2221 ctgaatttcg gcattatttc aacggaatca cagcaaaacc tgaaaccgca atggacgcca
     2281 ttcttacagg atatggagaa gaagctgggc gtgaaggtga acgccttctt tgccccagac
     2341 tacgcaggca ttatccaggg aatgcgcttc aataaagtgg atatcgcctg gtacggcaac
     2401 ctgtcggcaa tggaagcggt ggatcgcgcc aacggccagg tcttcgccca gacggtcgcg
     2461 gcggatggat cgccaggtta ctggagcgtg ttgatcgtca acaaagatag tccgatcaac
     2521 aacctgaacg atctgctggc gaagcggaaa gatctcacct tcggcaatgg cgatcctaac
     2581 tccacctctg gcttcctcgt ccccggttac tacgtctttg ccaaaaacaa tatctccgcc
     2641 agcgacttta agcgcaccgt caacgccggg catgaaacca acgcgctggc cgtcgccaac
     2701 aagcaggtgg atgttgccac caacaacacc gaaaacctcg acaagctgaa aacctccgcg
     2761 ccagagaagc tgaaagaact gaaggtgatc tggaagtcgc cgctgatccc aggcgatccg
     2821 atcgtctggc gcaagaatct ctccgaaacc accaaagaca agatctacga cttctttatg
     2881 aactacggaa aaacgccgga agagaaagcg gtgctggaac gcctgggctg ggcaccgttt
     2941 cgcgcctcca gcgacctgca actggtgccg attcgccagc tcgcactgtt taaagagatt
     3001 cagggcgtaa aaagcaataa cggactgaat gagcaggaca agctggcgaa aaccaccgag
     3061 attcaggcgc agctggatga cctggaccgc ctgaacaacg cgttaagcgc gatgagttcg
     3121 gtgagtaaag cggtgcagta atgtgctcga tcggtcccct ctcccttgag ggagagggtt
     3181 agggtgaggg ggaaatgcgc acgctgatgc cctcaccccg gccctctccc acagggagag
     3241 ggagaaaaca ttaaacggag ccaaacatgc aaaccatcac catcgcccca cccaagcgca
     3301 gctggttctc gcttctgagc tgggccattg ttctcgccgt gttggtcgtc tcgtggcagg
     3361 gcgcggaaat ggccccgctt acgctgatca aagacggcgg caacatggcg accttcgccg
     3421 ccgacttctt cccgcccgat ttcagccagt ggcaggatta cctcaccgaa atggccgtca
     3481 cgctgcaaat cgccgtctgg ggcaccgcgc tggcagtggt tctctccatc cccttcggcc
     3541 tgatgagcgc cgaaaacctg gtgccgtggt gggtttacca gcccgttcgc cgcctgatgg
     3601 acgcctgccg cgccattaac gaaatggtct tcgccatgct gttcgtggtc gccgtcggtc
     3661 tcggaccgtt cgctggcgtg ctggcgctat ttattcacac caccggcgtg ctctcgaaac
     3721 tgctttccga agcggtggaa gcgattgaac ccggcccggt ggaaggcatt cgcgccaccg
     3781 gcgccaacaa gctggaagag atcctttacg gcgtgctgcc acaggtaatg ccgctgctga
     3841 tctcctactc cctctatcgc ttcgaatcca acgtccgctc ggcgaccgtc gtcggcatgg
     3901 tcggtgcagg cgggatcggc gtcaccctgt gggaagcgat tcgcggtttc cagttccaac
     3961 aaacctgcgc cctgatggtg cttatcatcg tcacggtcag cctgctggat ttcctctctc
     4021 aacggttgcg taagcacttt atctgataag tgaggcattg atatctatgc acttgtctac
     4081 acatccgacc agctacccaa cacgctatca ggagatagcc gcaaaacttg agcaggagct
     4141 tcgtcaacac tatcgctgcg gcgactatct tcccgccgag cagcaactgg cggcgcgctt
     4201 tgaggtaaac cgccacaccc tgcgccgcgc catcgaccaa ctggtggaaa aaggctgggt
     4261 acagcgccgt cagggcatcg gcgtgctggt gctgatgcgc ccgttcgatt acccgctcaa
     4321 cgcccaggcg cgttttagcc agaacctgct ggaccagggc agccatccca ccagcgaaaa
     4381 actgctgtcg gtattgcgcc ccgcgtccgg tcacgttgct gacgcgctgg ggattaccga
     4441 gggtgagaac gttatccatt tgcgcaccct gcgtcgcgtc aacggcgtcg cgctctgttt
     4501 aatcgaccac tactttgcgg acctcaccct ctggccgaca ctgcaacgtt tcgacagcgg
     4561 ttcgctgcac gattttctgc gcgagcaaac cggaattgcg ctgcgccgca gccagacgcg
     4621 gatcagcgcc cgccgcgccc aggccaaaga gtgccagcgc cttgaaatcc cgaatatgtc
     4681 gccgctgctg tgcgtgcgca cccttaacca ccgtgacggt gaaagcagcc cggcggagta
     4741 ctccgtcagc ctgacgcgcg ccgacatgat tgaattcact atggagcact gaatgcacgc
     4801 agataccgcg acccgccagc actggatgtc cgtactggcg cacagccaac cggctgaact
     4861 ggcagcccgc ctgaacgcgc tgaacatcac cgccgactat gaagtgatcc gcactgccga
     4921 aactggcctg gtgcagattc aggcgcgaat gggcggtacc ggcgaacgct tttttgccgg
     4981 cgacgccacg ctgacccgtg ccgccgtacg cctgactgac ggcaccctcg gctacagctg
     5041 ggtgctgggg cgtgataaac agcacgccga acgctgcgcg ctgattgatg cgctgatgca
     5101 gcaatctcgc cactttcaaa acttatcaga aacccttatt gccccgctgg acgctgaccg
     5161 tatggcacgc attgccgcac gccaggccga agtgaacgcc agccgggtcg acttctttac
     5221 gatggttcgc ggagacaacg catgaccctg gaaaccgctt ttatgcttcc cgtgcaggat
     5281 gcccagcaca gttttcgtcg cctgttaaag gccatgagcg agccgggcgt gattgtcgcc
     5341 ctgtatcagc tcaaacgcgg ctggcagccg ctgaatatcg ccaccaccag cgtgctgctg
     5401 acgctggccg ataacgacac gccggtgtgg cttgccacgc cattaagtaa cgatatcgtc
     5461 aatcagagcc tgcgttttca taccaacgcg ccgctggtca gccagccgga acaggcgacc
     5521 ttcgcggtga cggatgaggc gatttccagc gaacagctca acgccctttc caccgacacc
     5581 gccgttgcgc cggaagcggg cgcgacgctg attttacagg tcgccagcct gagcggcgga
     5641 cgcatgttgc gtctcaccgg cgcgggcatt gccgaagaac gaatgatcgc tccgcagctg
     5701 ccagagtgca ttctgcacga actcaccgag cgcccgcacc cgttcccgct cggcatcgac
     5761 ctaatcctga cctgcggcga gcgcctgctg gctatcccgc gaaccactca tgtggaggtg
     5821 tgctgatgta cgttgccgtg aaagggggcg aaaaggcgat cgacgccgcc cacgccctgc
     5881 aagagagccg acgccggggc gataccgatt tgcccgaact gagcgtcgcc cagattgaac
     5941 agcagcttaa cctcgcggtg gatcgcgtga tgacagaagg cggcattgcc gaccgcgaac
     6001 tggcggcgct ggcgctgaaa caggccagcg gcgataacgt cgaagcgatt ttcctgctac
     6061 gcgcctaccg caccacgctg acgaaactgg cggtaagcga gccgctcgac accacggaga
     6121 tgcgtctcga acggcgtatt tcagcggttt ataaagatat tcccggcggc cagctgcttg
     6181 gcccaaccta cgactacacc catcgcctgc tcgatttcac tctgctggca aacggcgaag
     6241 cgccgacgct gaccaccgcc gacagcgaac aacagccgtc gccgcacgtt ttcagcctgc
     6301 tggcgcgtca ggggctggcg aagtttgaag aggatagcgg agcacagccg gatgacatca
     6361 cccgcacgcc gccggtttac ccctgttcac gttcttcccg tttgcagcag ttaatgcgcg
     6421 gcgacgaagg ctatttgctg gcgctggctt actccaccca gcgcggttac gggcgcaacc
     6481 acccgttcgc gggcgagatc cgcagtggtt acatcgacgt gtcgattgtg ccggaagagc
     6541 tgggatttgc ggtaaacgtc ggcgaactgc tgatgaccga gtgtgaaatg gtcaacggtt
     6601 ttatcgaccc gccggatgag ccgccgcact tcacgcgcgg ctacgggctg gtattcggca
     6661 tgagcgagcg caaagcgatg gcaatggcgc tggttgaccg tgcgttgcaa gccccggaat
     6721 acggcgagca cgcgacaggc ccggcgcagg atgaagagtt cgtgctggcg catgccgaca
     6781 acgtcgaagc cgcaggcttt gtctcgcacc tcaaactccc gcactacgtc gatttccagg
     6841 ccgaactgga gctactcaaa cgtctgcaac aggagcagaa ccatggctaa tctgagcggc
     6901 tacaactttg cctacctcga cgagcagacc aaacgcatga tccgccgcgc catcttaaaa
     6961 gcggtggcga tccccggtta tcaggtaccg tttggcgggc gcgagatgcc gatgccgtac
     7021 ggctggggaa ctggtggtat ccagctcacc gccagcgtga ttggcgaaag cgacgtgttg
     7081 aaggtgattg accagggtgc ggatgacacc accaacgccg tgtcgattcg caacttcttt
     7141 aagcgcgtga ccggggtaaa caccactgaa tgtacggacg atgcgacgct tatccagacg
     7201 cgtcaccgca tccccgaaac gccgctgacc gaagatcaga tcattatctt ccaggtgcca
     7261 atcccggaac cgctgcgctt tatcgagccg cgcgaaacgg agacccgcac tatgcacgcg
     7321 ctggaggagt acggcgtgat gcaggtgaaa ctgtatgaag atatcgcccg cttcggtcat
     7381 atcgccacca cctatgctta tccggtgaag gtgaacgggc gctacgtgat ggacccgtcg
     7441 ccgatcccga aattcgataa cccgaaaatg gacatgatgc ccgccctgca gctgttcggc
     7501 gcagggcgcg agaagcgcat ctatgcggtg ccgccgttta cccgcgtgga aagtctcgat
     7561 ttcgacgatc acccgttcac cgttcagcag tgggatgagc catgcgccat ctgcggatcg
     7621 acccacagct atcttgatga agtggtgctg gatgacgccg gaaaccgcat gtttgtctgc
     7681 tccgataccg attattgccg ccaacagagc gaggcgaaaa gccaatgaat caaccgttac
     7741 tttcggtcaa taacctgacc cacctttacg cgccgggcaa aggctttagc gatgtctctt
     7801 ttgatttatg gccgggggaa gtgctgggca ttgtcgggga atccggctcc gggaagacca
     7861 cgctgctgaa gtcgatctcc gcgcgtctga cgccgcagca gggggagatt cactatgaaa
     7921 accgttccct ctatgcaatg agcgaggccg accgccgtcg cctgctgcgc accgaatggg
     7981 gcgtggtgca tcagcatccg ctcgacggcc tgcgccgcca ggtgtcggca ggcggcaata
     8041 tcggcgagcg gctgatggcg accggggcgc gacattacgg cgatattcgc gctaccgcgc
     8101 agaagtggct ggaagaggtg gagattcccg ccaaccgtat cgacgacctg ccgaccacct
     8161 tttccggcgg tatgcagcag cgtttgcaga ttgcccgcaa cctggtgacg catccgaagc
     8221 tggtgtttat ggatgaaccg accggcgggc tggatgtgtc ggtgcaggcc cgcctgctcg
     8281 acctgctgcg cggactggtg gtggagctga acctcgcggt agtgattgtc acccacgatt
     8341 taggcgtcgc ccgcctgctg gcggaccgtt tgctggtgat gaagcagggg caagtggtgg
     8401 agagtgggtt aaccgaccgc gtgctcgacg acccgcatca tccgtataca cagctgctgg
     8461 tgtcatcggt tttgcagaat tgagccggtg ccggatgcgg cgtaaacgcc ttatccggcc
     8521 tacaaatgcg ctccccgtag gtcggataag acgcgtcagc gtcgcatccg acacccgaac
     8581 cacgaggcga aaaatgatta acgtacaaaa cgtcagtaaa accttcatcc tgcaccagca
     8641 aaacggcgtg cgcctgcccg tcctcaaccg cgcctcgctc atcgtcaacg cgggcgaatg
     8701 cgtggtgctc cacggccatt ccggcagcgg aaaatcaacc ctgctacgct cgctgtacgc
     8761 caactatctg cccgacgaag ggcaaatcca gatcaaacac ggtgacgaat gggtcgatct
     8821 ggtcaccgcg ccagcgcgca aagtggtgga gatccgcaaa accaccgttg gctgggtgag
     8881 ccagtttctg cgcgtcatcc cgcgtatttc ggcgctggaa gtggtgatgc agccgctact
     8941 cgataccggc gtaccgcgtg aagcctgcgc cgctaaagcc gcgcgtctgc tcacccgcct
     9001 gaacgtgccg gaacgcctgt ggcatctggc gccatcgacc ttctccggcg gcgaacagca
     9061 gcgcgtcaac atcgcccgcg gctttatcgt cgactacccc attctgctgc tcgacgaacc
     9121 taccgcctcg ctggacgcca aaaacagcgc cgccgtagtc gagctgattc acgaagccaa
     9181 agcccgtggc gcggccatcg tcggcatctt ccacgacgaa gccgtacgta atgacgttgc
     9241 cgaccgcctg cacccaatgg aagcctcttc atgattatca ataacgtcaa attggtgctg
     9301 gaaaacgaag tggtacacgg ttcactggag gtgcaggacg gcgaaatccg cgcctttgcc
     9361 gaaagccaga gccgcctgcc ggaggcgatg gacggcgaag gcggctggct gctgccgggg
     9421 ctgattgagc tgcataccga taatctggat aaattcttca ccccgcgccc gaaagttgac
     9481 tggcctgccc actcggcgat gagcagccac gacgcgctga tggtggcgag cggcatcacc
     9541 accgtactgg atgccgtggc aattggcgac gtgcgcgacg gcggcgatcg gctggtgaat
     9601 ctggagaaga tgatcaacgc catcgaagag acgcagaaac gcggcgtcaa ccgcgccgag
     9661 caccgtctgc atctgcgctg cgaactgccg catcacacca cgctgccgct gtttgaaaaa
     9721 ctggtgcagc gcgagccggt gacgctggtg tcgctgatgg accactcgcc gggccagcgc
     9781 cagttcgcca atcgcgagaa gtatcgcgaa tattatcagg gcaaatactc cctcactgat
     9841 gcgcagatgc agcagtacga agaagagcaa ctggcgctcg ccgcacgctg gtcgcagccg
     9901 aatcgcgaat ccatcgccgc cctgtgccgc gcgcgaaaaa ttgcgcttgc cagccacgat
     9961 gacgccaccc acgcccacgt tgctgaatct caccagcttg gcagcgtgat cgccgaattt
    10021 cccaccacgt tcgaagcggc ggaagcctcg cgcaagcatg gcatgaacgt gctgatgggc
    10081 gcgccgaata ttgtgcgcgg cggctcgcac tccggcaacg tggcagccag tgaactggcg
    10141 cagcttggcc tgctggatat cctctcttcc gactactacc ccgccagcct gctcgatgcg
    10201 gcatttcgcg tcgccgatga cgagagcaac cgctttacgc tgccgcaggc ggtgaagctg
    10261 gtgactaaaa atccagcgca ggcgcttaat ctccaggatc gcggggtgat tggcgagggc
    10321 aaacgcgcgg acttggtgct ggcgcatcgc aagggcaatc acattcatat cgaccacgtc
    10381 tggcgtcagg gtaaaagggt gttctgatga tgggaaaact gatttggtta atggggccgt
    10441 ccggctccgg gaaagatagc ctgctggcgg aactccgcct gcgggaacaa actcagctac
    10501 tggtggcgca tcgctacatc acgcgcgacg ccagcgccgg aagcgaaaac catatcgctc
    10561 tgagcgagca ggagtttttt acccgcgcgg ggcagaacct actggcctta agctggcacg
    10621 ctaacggtct gtattatggc gtcggcgtcg agatcgacct ctggctgcac gccggattcg
    10681 acgtgctggt caacggctca cgcgcccatc tgccgcaggc gcgggcgcgc tatcaatcgg
    10741 cgctgctgcc cgtctgttta caggtttcgc cggagatcct ccgccagcgc ctggaaaacc
    10801 gtggccgtga aaacgccagt gaaattaacg cccgcctggc gcgcgccgcc cgctatactc
    10861 cacaggattg ccatacgctc aacaatgacg gcagcctgcg ccagtcggtc gacacgctgc
    10921 tgacgctgat tcatcagaag gagaaacacc atgcctgctt gtgagcttcg cccggccacg
    10981 cagtatgata ccgacgcggt ttacgcgctg atttgtgagc taaaacaggc ggagtttgac
    11041 caccacgcat ttcgcgtggg ctttagcgcc aatctgcgcg acccgaacat gcgctatcat
    11101 ctggcgctgc tggatggcga ggttgtcggc atgatcggcc tgcatttgca gtttcatctg
    11161 catcatgtca actggatcgg cgaaattcag gaactggtag tgatgccgca ggcgcgcggc
    11221 ctgaacgtcg gtagcaagtt gctggcgtgg gcagaagaag aagcccgcca ggccggggcc
    11281 gaaatgaccg agctttcgac caacgtgaag cgccacgacg cgcaccgttt ctatctgcga
    11341 gaaggctacg agcagagtca cttccgcttc accaaggcgc tgtaacatga gtctgaccct
    11401 cacgctcacc ggcaccggcg gcgcacaggg cgttccggca tggggctgcg agtgcgcggc
    11461 ctgcgaccga gcgcggcgct cgccgcagta tcgccgccaa ccgtgcagcg gcgtagtgaa
    11521 gtttaacgag gcgatcaccc tgatcgacgc cgggctgcac gatctcaccg atcgctggtc
    11581 tcccggatcg ttccagcagt ttttgctgac gcattatcat atggatcacg tccaggggct
    11641 gtttccgctg cgctggggcg tgggcgatcc gatcccgatc tacggcccgc cggatgaaca
    11701 gggctgcgac gatctgttta aacatcccgg gctgcttgat ttcagccaca cggtggaacc
    11761 gtttgtggtg tttgatttgc aggggttgca ggtcacgccg ctgccgctta accactcaaa
    11821 actcactttc ggctatttac tggaaacggc gcacagccgg gtggcgtggc tgtctgacac
    11881 cgctggcttg ccggaaaaaa cgctgaaatt tttacgcaat aatcagccgc aggtaatggt
    11941 gatggattgc agtcacccgc cgcgcgcgga tgcaccgcgt aatcactgtg atttaaatac
    12001 cgtgcttgcg ctgaatcagg ttatccgctc gccacgggtg attctgaccc atatcagcca
    12061 ccagtttgat gcgtggctga tggaaaacgc actaccgtca gggtttgagg tggggtttga
    12121 tgggatggag attggggtgg cgtgatgaga gggaatgtgc gcgctggccc cctcacccta
    12181 accctctccc cagaggggcg aggggaccga ttgtgctcga tattgaatat tgcgctcgtt
    12241 ttctccctct ccccattggg gtgaggggcg atgcctgctc catacccaac ctcatcgccc
    12301 atactcatct tccattctcc gctcttcatc ctccagttgc cgacgctcct gatcaagctg
    12361 gcgctggcga tcgtccagct gcctgcggcg atcttcaaac tggcggcggc ggtcgtcata
    12421 ttgtctgcgc cgatcgtcgc tcacttcacg ctgccagccg tcgtcgcgcg aatcttcata
    12481 gtctcgccca cggtcagggt tataagcgtc attaatcgcc tgctgaatat taccaatagt
    12541 atcgtcgata atatcagcct gggccggaac ggtggacagc gtgagcaggg tgaataaaag
    12601 aaatagcgga aagcgtttca ttagccaacc tcaaaaagaa actctatcca cattagtcat
    12661 tactcatcca tgcaagtagt ggatgaatct caatttctcc gctgctctat tgccgtaatc
    12721 gcctccacgc gttgttgatg acgaccgcct tcgtactgtg cgcccagcca cgcatccaca
    12781 atcatttttg ccagttcgag gccaaccact cgtgaaccaa aagccagtgc aacttcactg
    12841 gcataatgcg gataatcagt gcgctctgac gacctggtgt ctttatcaat cacctcactc
    12901 attgaccatg aataaaaggc tggcacagca acgcagaatc ctgcggtgcc agcgacatga
    12961 ccgcccattg ctccggtacg ttttttaaaa agaaacaccg gttgcattga taaattgtgc
    13021 tca
//
LOCUS       DAZRUF010000113        13009 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZRUF010000113 DAZRUF010000000
VERSION     DAZRUF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13009
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.113"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            149..385
                     /gene="lpp"
                     /locus_tag="ACHXOL_002535"
     CDS             149..385
                     /gene="lpp"
                     /locus_tag="ACHXOL_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR6809492.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(448..1452)
                     /gene="ldtE"
                     /locus_tag="ACHXOL_002536"
     CDS             complement(448..1452)
                     /gene="ldtE"
                     /locus_tag="ACHXOL_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR6809493.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(1601..2017)
                     /gene="sufE"
                     /locus_tag="ACHXOL_002537"
     CDS             complement(1601..2017)
                     /gene="sufE"
                     /locus_tag="ACHXOL_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR6809494.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(2030..3250)
                     /gene="sufS"
                     /locus_tag="ACHXOL_002538"
     CDS             complement(2030..3250)
                     /gene="sufS"
                     /locus_tag="ACHXOL_002538"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR6809495.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(3247..4518)
                     /gene="sufD"
                     /locus_tag="ACHXOL_002539"
     CDS             complement(3247..4518)
                     /gene="sufD"
                     /locus_tag="ACHXOL_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR6809496.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(4493..5239)
                     /gene="sufC"
                     /locus_tag="ACHXOL_002540"
     CDS             complement(4493..5239)
                     /gene="sufC"
                     /locus_tag="ACHXOL_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR6809497.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(5249..6736)
                     /gene="sufB"
                     /locus_tag="ACHXOL_002541"
     CDS             complement(5249..6736)
                     /gene="sufB"
                     /locus_tag="ACHXOL_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR6809498.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(6745..7113)
                     /gene="sufA"
                     /locus_tag="ACHXOL_002542"
     CDS             complement(6745..7113)
                     /gene="sufA"
                     /locus_tag="ACHXOL_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR6809499.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(7661..7849)
                     /locus_tag="ACHXOL_002543"
     CDS             complement(7661..7849)
                     /locus_tag="ACHXOL_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR6809500.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            complement(7949..8359)
                     /gene="menI"
                     /locus_tag="ACHXOL_002544"
     CDS             complement(7949..8359)
                     /gene="menI"
                     /locus_tag="ACHXOL_002544"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR6809501.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(8356..11412)
                     /locus_tag="ACHXOL_002545"
     CDS             complement(8356..11412)
                     /locus_tag="ACHXOL_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR6809502.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            11801..12913
                     /gene="ydiK"
                     /locus_tag="ACHXOL_002546"
     CDS             11801..12913
                     /gene="ydiK"
                     /locus_tag="ACHXOL_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR6809503.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
BASE COUNT         3041 a         3498 c         3214 g         3256 t
ORIGIN      
        1 tttatctaaa acccagcgtt cgatgcttct ttgagcgaac gatcaaaaat aagtgccttc
       61 ccatcaaaaa aatattctca acataaaaaa ctttgtgtaa tacttgtaac gctacatgga
      121 gattaactca atctagaggg tattaataat gaaagctact aaactggtac tgggcgcggt
      181 aatcctgggt tctactctgc tggcaggttg ctccagcaac gctaaaatcg atcagctgtc
      241 ttctgacgtt cagactctga acgctaaagt tgaccagctg agcaacgacg tgaacgcaat
      301 gcgttccgac gttcaggctg ctaaagatga cgcagctcgt gctaaccagc gtctggacaa
      361 catggctact aaataccgca agtaatagta cctgtgaagt gaaaaatggc gcacattgtg
      421 cgccattttt ttgtctgcta tttaccacta ctgcgtcgcg cgtaacatat tcccttgctc
      481 tggttcacca ttctgcgctg actctactga aggcgcattg ctggctgcgg gagttgctcc
      541 actgctcacc gaaaccggat accctgcccg acgatacaac gctttatcga ctaacttctg
      601 atctacagcc ttattgtctt taaattgcgt aaagcctgct ggcagtgtgt atggcattgt
      661 ctgaacgttt tgctgttctt ctgctgatag tggtcgatgt acttcaacat aacgcatccc
      721 gttaggttcc acggaatatt tcaccggttc gttgatcact ttcaccggcg ttcccgtccg
      781 cacgctggag aacaaggctt taatatccgg tgcattcatg cgaatacacc ctgaactgac
      841 gcgcaaacca acgctgtccg gcgcactggt gccatgaatg aggtattcgc cattaccatg
      901 cgcgaggcgc agtgcgtaac gtcctagcgg gttatttggt ccggcaggaa cgactggcgg
      961 taatttaatg ccacgctcca gcgaacgctg acgaatgcct gccgtaggcg tccaggttgg
     1021 gttagggatt ttctgcccaa cacgcgtttc catcaccggc gtttccagcc cctgcaatcc
     1081 aatacctatt ggataaacct gcacaatatt ttctcccggc ggataataat aaaggcgcag
     1141 ctctgcaagg ttaacgataa tcccctgacg cggtgcatca ggtaataaca gttgtgaagg
     1201 aatagttatc gtcgtaccag gttttggcac cgggacgata gtgttattgg cttcaaggat
     1261 caacattgcc gcagtatcaa aacgtcgggc aatagcctga aggtttttat ccccttcttg
     1321 caccgtatac gtttgatttt gcccaaccag tcggcttccg gttggtggta gcggataatc
     1381 aaccgcccag gcagcctgga tggcgctaaa agcgccgata agcgtgagtg taagcaaaga
     1441 cgcgcgtttc attgtaaacc tcctgtattt gccggagact cacgctgaaa cgtcggatgg
     1501 cgcttatgtt cacctgaaac caaaacactc ctgtgcaggt cagtgtaaac attgaccatc
     1561 cggcaatgtg agccaaccgg atgaaagctg tccctttagt ttagctaagt gcagcggctt
     1621 tggcgcgaat tgcgcgaatc atcgcttcca gaccttgtga acgagatggg gtgagatgtt
     1681 gggtgagtgc cattttttca aaccacggac gcacatcgaa actgacaata tcctgcggcg
     1741 tcatctgatc gtagagaata aagactaccg caataagccc tttcacaatc gccgcatcgc
     1801 tgtcgccctg caattcaata attccgtgcg cattctggcg catgacaatc cacacctgac
     1861 tctggcagcc ctgaatgcta ttttgtgggc ttcggtcttc gtcgcgtaat tcagtcagac
     1921 gctggcccag ctcaataatg tagagatatt tctcttccca gttggcgcag cgtaaaaaat
     1981 tacgcagcaa tttttcttta tccggcaata aagccatagt gcctccctgt tatcccagca
     2041 aacggtgaat acgttgcagg ccggtcacca gacgatccac ttcttcatgg gtgttataca
     2101 tggccagcga cgcccgacac atcgcaggga cgttgtaata ggccattaat ggcatagcgc
     2161 agtgatgtcc ggtacgcaca gcaatgccgt aattatcgag aaaactgcca acatcatagg
     2221 cgtggtgttt accgagatta aaagcaataa cgccaagcct gttttgtggg ccatagagag
     2281 tgagatccgg tacagattcc agctgtgata gcgcataatg catcagattc tgttcatact
     2341 cggctatgtt attaagcccc agcgccgaaa catactccag cgccgcgcca agaccaataa
     2401 tgcccccggt attgggtgta ccggcttcaa accgccatgg tgctttggtc caggtagtgc
     2461 cttcactcag gctgacggtg gcgatcatag aaccgccccc ttcccacggc ggcatctcct
     2521 gcaacaaggc ttctttcaca taaagaatgc caattccggt ggggccatac agtttatgcc
     2581 cggagaacac gtaaaagtcg caatccagcg cctgaacatc caccggatga tgcatcaccg
     2641 cctgagcgcc atccaccagc acttttgcgc catgctggtg cgcaagcgtg attatttccg
     2701 ccagcggatt ttctgttccc agcacgttgg agacatgagt aattgccagc aggcgagttt
     2761 tctcatcaaa cagcgtaggc agcgtctcca gttgcagcgt accgtcgggg ttgagaggga
     2821 tcacacgcag ctctgcgcca acgcgtgcgc aaagcatctg ccagggaaca atgttagcgt
     2881 ggtgctccat ctgactgatg atgatgttat cgcccgcccg cacgttgctg ttgccccaac
     2941 tattggcgac cagattgatc ccttccgtcg tgccgcggac gaacaccagc tcttccgccg
     3001 aacgggcatt aataaacagc gatgcccgct tgcgcacgtt ctccattttc tcggtcgcct
     3061 gggcgcttaa ggtatgaata ccgcgatgca ccgccgcgta gccgtgacga taaaactcag
     3121 cctcggcgtc aatcacctgg cttggtttct gcgcactggc ggcgctgtcg agataagcca
     3181 gcggcaaacc gttcacctca cgagaaagca ccggaaagtc ggcccgcact ttgtcgacgg
     3241 aaaaagtcat cttgcacctc ctggcagccg ttgaccgatt cgggccagca cctgctgttt
     3301 aagcccctca tcacgcagtg cttccgtcag ttcggcagca aaggcgtaaa tgatcatctg
     3361 ctgggcatcc tgctgattga tcccgcgcga gcgcagatag aacatctgtt catcatcaat
     3421 acgccccacc gtcgcgccgt ggctgcattt cacatcatct gcatagattt ccagctgcgg
     3481 ttttgtatcc acttccgcca ttttgcccat cagcagatta ttgttggtca tctgaccatc
     3541 cgttttgatg gcgtgctgcg cgacgttgat caaaccgtta aataccgcgc ggcctttgtc
     3601 gctgacgata gttttgtgca actgtcggct gttacaaaaa cctttattgt gttccagcca
     3661 ggtacgggta tcacacacct cgtttttcac cggcatcgcc aggctattga tccgcagcgt
     3721 gctgttttcg ccattgagtt gcgtactggt gttgtgtcgt aacactgcgc cacccagcag
     3781 gaaactatgg ctaaatgcgg tggcatcctc agccagcaac aaatcgttat gagcaaagtg
     3841 gtgactgagc gggttttcaa acgccagctt gatatgctgc aagtgggcgt tcgctgcgac
     3901 attgatagtg aaccgtgccc cggtaaaatg acgagcatca ttaaggctga caaaatgttc
     3961 gatcaccgtt gcttcggcac cttccgccag atccagatga tgccggtaat gggcagtgtt
     4021 cacctcttca cctgccacgc cctgggtgat atgcattaac agcaatggct ttgccggccg
     4081 ttgaccgcgc ttcacggcga tatgcgtcac gctttgtgcc aggctttccg tcaaatgcag
     4141 aaacacttcc gcctgaatag cgtcgggtaa cccctgacgg tcgtcgttaa tgctcacttc
     4201 atatccgctg ccttcaattg catcgctcag cgcgggcacg taacgtccat cgacaaacac
     4261 cagccgcacg gcgtctaacg ttaacgctaa ggcatcacgc tgctgtgggg atatatctcc
     4321 cgcaatgctg acaaactgac tattggtcag cccttccagc ggcgtatatt tccagttttc
     4381 atgtttacgt gtcggcagtc cggtacgcag caattgttgt aaatgctgct gtgcttgcgg
     4441 tgagcgtttt gtcccttcag cttcaaacaa gtgatgccac tgttgcagcg cattactgct
     4501 gttcggtaag ccagccataa ccctgctcct ccagttgttt gaccaacgtg aaatcgccgg
     4561 atttcacaat tcgtccctga tatagcacat ggacgtaatc aggcttgatg tagtcgagaa
     4621 tgcgttggta gtgcgtaaca atgatgaatg agcgcttgcc gtcacgcagc gagttcacgc
     4681 catcggcaac cacttttaat gcgtcaatat ccagcccgga gtccgattca tcaagaatgc
     4741 ataactccgg ttccagcacc gccatttgca aaatatcgtt gcgctttttc tcgccgccgg
     4801 aaaaaccaac gtttaccgaa cgggttaata aatcttccgg catcttcagg agagcgattt
     4861 tctcttccat caaatcctga aaatcaaagc ggtcgagcgt ttcctggccg cgatagctgc
     4921 gcaccgcatt aagtgccgtt tgcaggaaaa actggttact gacgccggga atctccaccg
     4981 gatactggaa ggccataaag atgccttcgc ctgcacgatc ttccggcgac agcgcaagca
     5041 aatctttgcc tttgaactca accgtgccgc ccgtcacttc ataatcttct cgcccggcaa
     5101 gcgttgccga taaggtactt ttgcccgaac cgtttggccc cataatggcg tgaacttcgc
     5161 cgggacgaac gtcgaggctt aatccacgca ggatagcttt atcttccacg ctgacgtgta
     5221 aatctttaat acttaacatg tttattcctt atccgacgct gtgttcaaga ctgatggcga
     5281 ggagtttttg tgcttcaacg gcaaattcca gcggcagttc tgagaacacg tctttgcaga
     5341 aaccgttaac aatcatcgag atggcgtctt cttcgctaat cccgcgttgc aggcagtaaa
     5401 acagttgatc ttcaccaata cgtgatgtcg ttgcctcgtg ctccagttgt gcgctgttat
     5461 tgcgacattc gacatacggg aaggtatgcg ccccacaatt agcgccaatc agcattgagt
     5521 cgcactgagt gaaattgcgc gcattggttg ccgtcggcat gattttcact aagccgcgat
     5581 aactgttctg actatgtccg gcagagatcc ctttcgagat aatggtcgat ttggtgtttt
     5641 taccgatgtg gatcatcttg gtgccggtat ccgcttgctg atgaccgctg gtcagcgcca
     5701 ctgagtaaaa ctcaccaatg gagttatcgc cgcgcaaaat gcagctggga tatttccacg
     5761 taatcgctga cccggtttct gattgcgtcc atgacatttt gctgttttcg ccttcgcaca
     5821 aagcacgctt ggtgacgaaa ttgagaatac cgccggtgtt gttatcgcca gggaaccagt
     5881 tttgtaccgt ggaatatttc acctcggcgt ttttatggat gatgacttcc accaccgccg
     5941 cgtgtaactg atagctgtca cgcaccggag cggaacagcc ttcaatgtag ctgacgtagc
     6001 tgtcttcgtc ggccaccaga atggtgcgct caaactgccc ggttttttcc gcgttaatgc
     6061 gaaaataggt ggaaagttcc atcgggcagc gcacgccttt aggcacataa ataaacgtac
     6121 cgtcagaggc taccgccgca ttaagcgcgg caaagaagtt gtcatttccc ggcaccacag
     6181 tgccgagata tttacgcacc agttccggat gatcgtggat cgcctcacca aaggaacaga
     6241 aaataattcc ctgctccgcc agtttttcgc gataagtagt ggcaaccgaa acagagtcga
     6301 aaatggcatc caccgccacc tctttgcctt cccgcacggg aacgcccaac tgctcaaacg
     6361 ccgcctccac ctctttactt aaaaaggcgt tcgcgccagt ttgctgcacc gcgccaggtt
     6421 cagacgcgca agtgtcgtca caattaccgc acgatggtgc tgagtagtaa ctgtaatcct
     6481 gataattcag cttgtcgtag tgcgctttca accagtgcgg ttcttccatc tccagccatg
     6541 cgcgataggc gtttagacga aactccagca tccactccgg ctcattacgc ttcgccgaaa
     6601 ttgcgcgcac cacctcttcg tttatcccct ttgccagctc atcggtggct aactgggtga
     6661 agaatccttc tttataattc agcgggccgc cggtccaggt tttgacatcg tcagttgctt
     6721 cagtattacg agacatagta ccgcctatac cccaaagctt tcgccacagc cacattcatt
     6781 ctgggcttta gggttgtgaa atttgaatat ctgattaagt ccttcacgaa cgaaatcgac
     6841 ttccgtgcca tcaataaacg gcatcgcttg cagcgggaca aacagcttcg cgccgtcgtg
     6901 ttcaaacagc agatcgtctt tgtccggctc gctaacactg tcgagcacat agccaaagcc
     6961 cgcgcagccc gtttgcttca cgcctaagcg cacgccgacc atacccggct gctttgccac
     7021 cagctcacgg atgtgtatcg ccgctgcggg tgtcagcgtt aagccttgcc aggcgaaatc
     7081 ttgtgggtta aaggttcctg aatgcatgtc catcgattta cctcacttca tcgctttcag
     7141 cgtataacag catgctagtg ataatgatta tcagttcaac ccagcaaacg caggggcttt
     7201 accgtaaaac atgctttttg cctgctttta ataagcatag accctgatgt gtgggttaac
     7261 aggcacgcta agaattaggt atctcattgt tagataatgg ttattatcta aggagcatta
     7321 aggctgtgat gaaaaagaaa aagttgtatt gaaaatgact atttaagaga taggtaaaaa
     7381 agtgcagcgt tcagaaataa gaaaaccctt aagtctgtgc gacacaggct taagggtttc
     7441 taccccatcc ggcgcttatc tccggcactc tcagtggctt agctcttgaa ggggcgataa
     7501 gaataatctc ataaagctaa cccgccgttt taacacaaac tgcgattagt attatttttg
     7561 aacaatatca ggcggtagat aagcagtatt aagaaggtca tcgaacctgg acggaggtta
     7621 atccaggtcg atttggcgaa cttgcggcat taagtcagga tcaatgcacg cccagacgcc
     7681 aggcaaagta gatttcttct tttaattcag tagaagagag agtaagcagg tcagcaaatt
     7741 caagttctag ttgtttcaga cgtttgagat attgggcagg tgaaagattg ctctggtcac
     7801 ggcgtaaaaa ttcaatggcc agctgggtgg gatcaagttg agtagacata gcatcctcgc
     7861 ttttagacaa gacgtgcaca gtataccacc gtttactgtg cagataatga ccaaaagcaa
     7921 tatgcgtcac acttttctgg tgacaacgtc acaaaatggc ggtcgtcaat cgtgacgaac
     7981 agcacaaacg ccctttctca tcgaagattt caatctgcca gacctggtga cgcgaaccga
     8041 gatgcaacgg tttacatatg ccgcgcaccc gcccttctcg tgccgagcgg acgtgattag
     8101 cattgatttc cagaccaacc actttttgct caccttcggt acataaataa ccggcaacgg
     8161 aaccgatact ttcggccagt accacggatg ctcctccatg cagcaacccg aaaggctgct
     8221 ttgtccgcga gtctactggc attgtcgctt caagggtgtc atcaccaata tgttcaaagc
     8281 gaatatccag gaaccccacc atgtttcctt cacccatagc attcagtgct tccagggtga
     8341 ttttccgttt ccatatcatt taataatctc cagtaaagcc tgcacaggat ggcgtacccc
     8401 cgtgccttca acccgtttta cctggctacg gcaggaatat ccggtcgcca gacagcggtt
     8461 acgcggcagt cgctgcatag cctgatgcca ggataactca tagatcccga gcgaattttc
     8521 atggtttttc gcttcatgtc cgtaagtccc tgccatgccg cagcaaccca cgctgacatt
     8581 ttccagtttc gcgccaaaac gggcaaatat cgcggcccat tgtgctggcg cgcccggcaa
     8641 agcggtaacc tcggtacagt gaccaaagaa ataccatgat tcaccgctga ctgtagccac
     8701 tggatgtgac tcaagtgcgc ttgccagcca ttcattcgca agtaagacgt taaactcgcc
     8761 acgttcctcg cccagcgcca gtttatattc atcgcgataa caaagtacca gcgccggatc
     8821 gacgcccacc attggcatcc caagcttagc catacggttg aggaaatccg ccgtcttttt
     8881 cgccgtcttc gcaaaacgat taagaaagcc tttgatatgc tgggctttgc catttggcga
     8941 aaatggcagt aacacaggct ggaaacctaa tttttcaacc agacggacaa aatccgccac
     9001 cacttgcgca tcgtaatagc tggtaaaggg gtcctgcacc accaacaccg tgcgcgcttt
     9061 ctgctctgca ttgagagctt caagctgttc cagcgtcatg tttgccgagc gatgccccac
     9121 catttgttgt tgtagcgagg ggaccgacag cagtggcaaa tcaaccatgc cgatatgttt
     9181 ttccgagagt ttgcgcacca gcggctggtt aatgaagaag ttaaaggttt tcggcgcgcg
     9241 tgccatcagc ggtgcgtagc tctcgaccgt agcgacgagg tggtcgcgca gcgggcgtaa
     9301 ataacgggtg tgatagagct gcaggaaacg agagcgaaac tccggcacat caattttgat
     9361 ggggcactgg gtcgaacacg ctttacaggc cagacagccc gacatcgcct ctttgacttc
     9421 gtgtgagaag tcatattcgc ctttattcgc atgccagcta ttgcgcgtgc gggcaattaa
     9481 cgtccgtaaa ctgacgccgg attcaggcag ttcttgttcc agtttgagtg gatcaacgcc
     9541 gcgatccgcc aacaaacgca gccattcacg caccagcgtt gcgcgccctt tcggtgaatg
     9601 aatccggttc tgggtgatct tcatcgacgg acacatcgga ctacgggcat caaagttgaa
     9661 gcataaaccg ttgccgttac actccatcgc accgcgccac tgctggcgta ccgcaatggg
     9721 gatctgccga tcgaatgtac cgcgcttcac cgcgtccact ttcatcatcg gcgcatcgag
     9781 accttctggc gggcaaatct tccctgggtt gagtcggtta tgcgggtcaa atgccgcttt
     9841 cactttgcgc agttctgcaa aaagttcctc accgaaaaac gccgggctgt attcagcgcg
     9901 aaaacctttg ccgtgctcgc cccacaacaa accaccgtat ttcgcagtca gcgccaccac
     9961 gtcatcagag atttgcttca tcaaaatctc ttgttgcgga tcgcacatat ccagtgccgg
    10021 acggacgtgc aaaacacctg cgtcgacgtg accgaacata ccgtagctta agccgtggct
    10081 gtcgagcagc gcgcgaaatt cagcaatata atcggccaga tgttccggcg gtacgcaggt
    10141 atcctcagca aacggaattg gcttagcggc acctttggca ttgccaagca gaccaacggc
    10201 ttttttgcgc attgcataga tacgttcaac ccccgccagc tcgcggcaca cctgccagcc
    10261 aatcacacct gcctgttggc tggcaatcag ctcatcaagc cgcacacaga gtgcattaac
    10321 tcgctcgtca atcagcgcct catcatcacc agcaaattcc acaatgttca gcccgagcat
    10381 ctctttgtca ggcacatcgg taatcaactc gctgacggaa tgccagacaa tatcttcccg
    10441 cgccagattc agcacttttg agtccaccgt ctctaccgaa agcgcccgcg cctcaaccat
    10501 aaacggcgcg ttacgcagcg cggagtcaaa agagtcatat ttgacgttca ccagacggcg
    10561 cactttaggc aagggcgtaa tatccagccg cgcttcggta ataaaggcca gcgtcccttc
    10621 tgaacccgtc agaatgcgcg tcaggtcgaa ctcggtcatc tcatcgttaa agacatgacg
    10681 cagatcgtaa ccggtaagaa agcggttaag tttggggaag ttgtcgatga ttaactggcg
    10741 ttgcagacgg caacgttgat aaaccgtgtt ataaattcgc ccgattgtgg tattggtttt
    10801 acccagcgtt tccgccagtt cgacgggtaa aggttgcgta tcgagaatat cgccccccaa
    10861 caacaccgcg cgtacgccaa gtacgtgatc tgacgttttg ccatagacca gcgatccctg
    10921 accggatgca tcggtattga tcatcccgcc gagcgttgcc cggttgctgg tcgaaagttc
    10981 tggtgcaaaa aagtagccga acggtttcag gtactgattg agttgatctt ttatcacccc
    11041 ggcctcaacg cgcacccagc cctcttcagg gttaatttcg atgatgcggt tcatatggcg
    11101 ggacatatca acaataatcc cctggttgag cgcctgaccg ttagtgccgg tgccgccgcc
    11161 gcggggggta aagatcagcg atgaatagcg ttcctgcgcg gcaagacggg cgatcagcgc
    11221 cacatctgcg gttgaacgcg gaaataccac cgcatcgggg agaagttggt aaatactgtt
    11281 gtcggtcgac attgtcagac gatcggcata acttgtcgcc gtatcgccgg taaaaccttg
    11341 ttgctccagc tcttgcaaaa aattaagcac cagttgaacg acgccgggtg cctgggaaat
    11401 ctgtggaatc attatattga ccctttcctg cggtctgtga tgtaggtcga tacactattc
    11461 tttcaggcgg ctgcaatagc gcactgaaag gtgatgttta tttactctat ggatttcgag
    11521 ttgcaggaag gcggcaagcg agtgaactcc aggagcttac aatagtaagt gactggggtg
    11581 aacgaacgta gccgcagcgc atgcaacttg aaatacgacg agtaaatcgt ttgcgtgttg
    11641 cctgagttgt tgtaccacat ttttttctaa cacgcccatc agaattaagg gcagaatcgg
    11701 cctgttaaaa accgctgaaa ttgctcatca ttatgcaggt gagtttcgcg tgttcacgtc
    11761 gcgtcgacga tttgacgcac aaaaaaggtg aaaagtagtt atggtaaatg ttcgtcagcc
    11821 cagggatgtc gcacaaattc tgctttcggt gctgttttta gccatcatga ttgtggcatg
    11881 tctgtggatt gttcaaccct ttattctcgg ctttgcatgg gccggtacgg tggttatcgc
    11941 cacctggccg gtattgttac gtttgcaaaa gatcatgttt ggccgccgct ctctcgccgt
    12001 tctggtgatg acgctgttat tagtgatggt gtttatcatc cctattgctt tgctggttaa
    12061 cagtatcgtc gacggcagcg gcccgctaat taaagccatt tccagcggcg acatgacgtt
    12121 acccgatctg gcgtggctta ataccattcc ggtgattggc gcgaagctgt atgcaggctg
    12181 gcacaacttg ctggatatgg ggggtacggc gatcatggcg aaagtccgcc cttatattgg
    12241 caccaccacc acctggttcg ttgggcaggc ggcgcatatc gggcgcttta tggtgcattg
    12301 tgcgctgatg cttctcttca gtgccctgct gtactggcgc ggtgaacagg tggcacaagg
    12361 cattcgccat tttgcaaccc gtctggcagg cgttcgcgga gatgccgccg tgctgctggc
    12421 ggcacaggct atccgcgcgg tggcgctggg tgtggtggtg acggcgttag tacaggcagt
    12481 gcttggcagt atcggcctcg ccgtatccgg cgtaccttat gcaacgttgc taacggtgtt
    12541 aatgatcctc tcctgccttg tccagcttgg cccgttgccg gtactgattc cggcgattat
    12601 ctggctctac tggaccggcg ataccacctg gggaacggta ttgttagtgt ggagcggtgt
    12661 ggttggcacg ctggataacg tcatccgccc aatgttaatt cgcatgggtg ccgatttacc
    12721 gctgatcctg atcctctctg gcgttattgg tggtttgatt gctttcggga tgatcggtct
    12781 gtttattggt ccggttctgt tagccgtttc ctggcgtctg tttgccgcgt gggtggaaga
    12841 agtcccgccg ccgacggacc aaccggaaga aattctcgaa gaacttggcg aaatcgagaa
    12901 accgaataag taatttctca tcaggcggct ctgccgcctg attgttaatc accgctaatt
    12961 gatatttgct tgctcttccc catattttcc tgcttaccgc cattcagct
//
LOCUS       DAZRUF010000114        12998 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZRUF010000114 DAZRUF010000000
VERSION     DAZRUF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.114"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHXOL_002547"
     CDS             <1..191
                     /locus_tag="ACHXOL_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809504.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHXOL_002548"
     CDS             191..1393
                     /locus_tag="ACHXOL_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR6809505.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLVLAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHXOL_002549"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHXOL_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR6809506.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHXOL_002550"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHXOL_002550"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR6809507.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHXOL_002551"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHXOL_002551"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR6809508.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHXOL_002552"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHXOL_002552"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR6809509.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHXOL_002553"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHXOL_002553"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR6809510.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHXOL_002554"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHXOL_002554"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR6809511.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGAPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHXOL_002555"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHXOL_002555"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR6809512.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3038 a         3227 c         3576 g         3157 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggtgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc attaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gatttcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctggtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcgc tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgctggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZRUF010000115        12936 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZRUF010000115 DAZRUF010000000
VERSION     DAZRUF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.115"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(37..189)
                     /locus_tag="ACHXOL_002556"
     CDS             complement(37..189)
                     /locus_tag="ACHXOL_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR6809513.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHXOL_002557"
     CDS             complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHXOL_002557"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR6809514.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1263..2975)
                     /gene="cysI"
                     /locus_tag="ACHXOL_002558"
     CDS             complement(1263..2975)
                     /gene="cysI"
                     /locus_tag="ACHXOL_002558"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR6809515.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2975..4774)
                     /gene="cysJ"
                     /locus_tag="ACHXOL_002559"
     CDS             complement(2975..4774)
                     /gene="cysJ"
                     /locus_tag="ACHXOL_002559"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR6809516.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5093..5455
                     /gene="queD"
                     /locus_tag="ACHXOL_002560"
     CDS             5093..5455
                     /gene="queD"
                     /locus_tag="ACHXOL_002560"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR6809517.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5533..6804
                     /locus_tag="ACHXOL_002561"
     CDS             5533..6804
                     /locus_tag="ACHXOL_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR6809518.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6795..7055
                     /locus_tag="ACHXOL_002562"
     CDS             6795..7055
                     /locus_tag="ACHXOL_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR6809519.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7072..7647
                     /locus_tag="ACHXOL_002563"
     CDS             7072..7647
                     /locus_tag="ACHXOL_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR6809520.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7795..8655)
                     /locus_tag="ACHXOL_002564"
     CDS             complement(7795..8655)
                     /locus_tag="ACHXOL_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR6809521.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8652..9431)
                     /locus_tag="ACHXOL_002565"
     CDS             complement(8652..9431)
                     /locus_tag="ACHXOL_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR6809522.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9409..10359)
                     /locus_tag="ACHXOL_002566"
                     /pseudo
     CDS             complement(9409..10359)
                     /locus_tag="ACHXOL_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10346..11779)
                     /locus_tag="ACHXOL_002567"
     CDS             complement(10346..11779)
                     /locus_tag="ACHXOL_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR6809523.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGINLAG"
     gene            complement(11849..12665)
                     /locus_tag="ACHXOL_002568"
                     /pseudo
     CDS             complement(11849..12665)
                     /locus_tag="ACHXOL_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT         2995 a         3531 c         3241 g         3169 t
ORIGIN      
        1 caattcagta cgcggggttt tacccccgcg tggtggctac ttcttcgatt cgtaagcgag
       61 aactgcctta aactccatac cacgttctct gatactcaac tcacacaacg agtcgcctac
      121 catcagcgtg aatcccagta ctgtaaaaca cagcactatg attgccacaa gggcatattt
      181 tgtcagcatg tcttgcctcc ctgcaaagaa gagactaaca ttcgaattgt ttgggttcga
      241 acgctggcct caggttgata gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg
      301 aatgcttaac gccagacagc ctcaagcacc cgacgccatt ctatacctga taattcttcc
      361 agcggcgttc acgtttaaaa tcaggaatac tgctcgcaaa ataaaaacgc ccccatcatt
      421 tctgacagag gcgtttaatt tatccggcaa tatttaccct tcgtgcaatc cacattccct
      481 ttttaagcca aagaaacgtg tttcttcttc cgacatgcca ggttcccatt tacgggttgt
      541 atgggtatca ccgaccgata aatatccttc atcccataat gggtgatatt tcaggccatg
      601 tttttgcagg tactgataaa tagttcggtt atcccagtcg ataatcggca gcactttaaa
      661 tacgccacgc tgaattgcca gcaccggtaa attggcgcga ctgccggatt gttcacggcg
      721 caggccagca aaccaggttt gcgcgttaag ttctttcaga gcccggttca tcggttcgac
      781 tttgttgatg tcattgtact tttcaatgcc ttcaacgccc tgctcccaca gttttccgta
      841 gcgtgcttcc tgccaggctg cgctttcggt agcacggtac actttcaggt tgagcttgag
      901 tttgtccgtt aactcgtcaa taaagcggta ggtttccggg aacaagtaac ccgtatcggt
      961 gaggatcacc ggaatatccg ggcgaatttg attcaccaga tgcaggctca ccgccgcctg
     1021 aatgccaaag ctggaagaaa gtacatattc accgggcaga ttatccagcg cccaggctac
     1081 gcggccttca gcgtccagtt tttccagttc ggcgttagtt tccgccagcg ccagaatacg
     1141 atctaccttc ggcagttcgt tcagggcgtt tagatcgagt ttggacatag attcctcact
     1201 gtttgccttg cctgatgcga cgctcacgcg ttcttatcag gcctacaaga ccgggctgat
     1261 ggttaatccc acaaatcgcg cgccggatcg agcaccgggc gaatgatgcc cgcacgcacc
     1321 gtaaagtcgc cgaagccttc acccgcttcg cgctctttcg cccagcgccc tatcagttca
     1381 tcaagcgacg ccaggatttc cggctcggtg atgttttctt tatacatccg tgggatacgt
     1441 gtcccaatgc ggttgccgcc aagatgcagg ttgtagcgac ccggcgcttt acccaccagg
     1501 cccacttccg ccagcatcgc gcgaccacaa ccgttcgggc agcctgttac acgcatcacg
     1561 atatgctcat cgctgacacc atgtttcgcc attaaattat cgatgttgtc gataaaagac
     1621 ggcaggaaac gctctgcttc cgccatcgcc agcgggcaag tcgggaatga cacgcaggcc
     1681 atcgagtttt cacgctgcgg cgtgacggca ttcattaacc cgctctcttt ggcgatcttc
     1741 tcgatcttcg ctttctcgct ttccggtaca ccggtgatga tcagattctg gttcgccgta
     1801 atgcggaaat cgcctttgtg gatcttcgcg atctccagca ggccggtttt cagcggacgc
     1861 cccggataat caaggatgcg accattttcg ataaacagcg tcaggtgcca gttatcatca
     1921 atgcccttaa cccagccaat acgatcgcct cgtccggtga actcatatgg acggatcggt
     1981 tcaaatttga tccccgcgcg acgctccact tccgctttaa acgtctcaac ccccacgcgc
     2041 tccagcgtgt atttggtttt ggcattttta cgatcggttc ggttacccca gtcacgctga
     2101 gttgtcacga cggcttcggc caccgccagc gtatgctcca gcggcagata gccaaactcg
     2161 ctcgccgtgc gggcgtaggt tttcttgttg ccgtgttcga tagaaagccc accgcccacc
     2221 agcaggttga agcctaccag cttgccgttt tcggcgattg ccacgaagtt catgtcgttg
     2281 gcatgcagat cgatatcgtt ctgcggcggg atcactaccg tggttttgaa cttacgcggc
     2341 aggtaggtct gaccaaggat cggttcttca tcagtagtgg cgactttttc ctggtcgagc
     2401 cagatctccg catacgcgcg ggtacgaggc aacagatgct cagaaatctt cttcgcccac
     2461 tcgtacgctt ccgcgtgcag ctgcgactcg taagggttcg aggtgcagag tacgttacgg
     2521 ttcatgtcgt tagctgtcgc cagcgcatca agaccgaccg agtgcagcat ctggtgcacc
     2581 ggtttgacgt tctttttcag aatgccgtgg aactgaaacg tctggcggtt ggtcaggcga
     2641 atgctgccat agatggtgtt ttcaccggca aatttgtcga tcgcctgcca ctgtttagtg
     2701 gtaatcaccc cacccggcag acgacagcga agcagcatcg cgtggcgcgg ctccagcttc
     2761 tgttcagcac gttcggcacg gatgtcgcgg tcatcctgct gatacatgcc gtggaagcgg
     2821 atcagcagga agttgtcgcc cttaaagccg ccggtcagac cgtcgtttaa atcttccgca
     2881 atggtgccgc gcaggtagtt gctttcaagc ttcatgcgct cggcgtctgt cagttttcct
     2941 tcgaccacta aaggccctgg atgtttttcg ctcattagta gacatctcgc tgataacggc
     3001 gctctacgcg cagctcactt aaaaattcat ccgccgcttc ggtgtccatg ccaccaaatt
     3061 cggcaatcac ttccagaagt gcctgctcaa cgtctttcgc catgcgatta gcgtcgccgc
     3121 agacataaat gtgggcaccg tcattgatcc agcgccacag ctccgcgccc tgttcgcgca
     3181 gtttgtcttg tacataaact ttttcttttt ggtcgcgcga ccaggcaaga tcgatacgcg
     3241 tcagcacgcc ctctttgacg tagcgctgcc actccacctg gtagaggaaa tcttccgtaa
     3301 agtgcgggtt gccaaagaac agccagtttt tacccggcgc ttcgtcggcg gcgcgttgct
     3361 gcataaaggc acggaacggc gcgatgccgg taccggggcc aatcataatc actggcgttt
     3421 gcgggttagc gggcaggcgg aaattgtcgt tatgttcgat aaatacgcgg acttcgccct
     3481 cttcttccac gcgatccgcg aggaagctgg aggcaccacc ggcacgggcg cggccttcca
     3541 cgtcgtaacg caccacacca acggtgacgt gtacttcgtt ctcgacttcc gcctgcgagg
     3601 aggcgatgga atacaggcgc ggggtcagcg ggcgcagcag gttaatcagc gcttcggcat
     3661 ccagttgcgc cggagagaaa cgcaccatgt cgacaatcgg cgtggtcgcg gcgtaatgct
     3721 gtaacttcgc tttatcgccc accagcggca gcagcgtttc gctgcgggta agcgtggcat
     3781 agttctcaac aatgttggcg gtgttgacgg tcagttcgaa gtgccactgt agcgcttcgt
     3841 tcagaggcaa cgttttgccc tcgacggtga caggttcatc gcctttcagc cacagcagtt
     3901 cgacaagttc tttcaccagt gccgggtcgt tctgatacca gacacccagc gcgtcacccg
     3961 gctggtaacg caggcctgaa tcgcctaagt caatttcgat atgacgaacg tctttttcag
     4021 agttacgccc ggtaattttc tggttaaccg aaaggctcgc caccagcggc gcgtctttgc
     4081 tgtacgggct ggtgtggatt tcatttaccg cgccagtagc gacggattgc gaaggtgccg
     4141 cgacaggcgc acgcgattta agcgcatcaa ccacgcgggc gcgccactcg ctggcagcag
     4201 cctggtattc aacatcggca tcgacacggt cgagcaggcg ttcaccaccc agttccgcca
     4261 gcttgctgtc gaaatctttc ccggactggc agaaaaattc ataagagcta tcgccgaggc
     4321 taaacacggc aaacgcggtg ttttccagct ttggcgcttt tttggagaac aggaacttat
     4381 gcagcgcgac ggcttcttcc ggcggttccc cttccccttg cgttgacgtc actacgatga
     4441 gcagtttttc gctggcgatt tgtttgaatt tatagtcgcc cgcgttcacc agcttaacgt
     4501 tcagttttgc tgctaataaa tcatcgcgta atgcttcagc aacccggcgc gcattgccgg
     4561 tttgcgaggc ggagataata gttatacccg gcatttctgc ggctggcgct ggcgtcgctg
     4621 caagcgcagc aggctgctga ttgagtacgc cccagaaata gccagaaacc caggcaagct
     4681 gggtaggcgt taaatcggtc gtggccgcct gaaggcgtgc cagttgctcc gggttcaacg
     4741 gaagcaacgc ggaaggtggg acctgtgtcg tcatgcgtcg ttatgttcca gtaagcaaag
     4801 ctgtttctgc gccctgtcag cgcccataaa acagaagaga agataaggtt aacggggtga
     4861 acggtgtgga ttaaagacgg gatagcgata actaataacc aaatcgacta acctgtttta
     4921 gcaatagtct ttaacaacaa aatagattaa ccaacccaat gaaaaacaaa tgaatttagc
     4981 caatcattaa gataaatcag cgattttgcg caacaaagtc gttttagata atgcgaaaaa
     5041 acagcctttc cggtactcta cggcggtttt atcgtcctgt agagaaatta tgatgtccac
     5101 cacgttattt aaagatttca ccttcgaagc cgctcaccgc ttaccacacg tcccggaagg
     5161 gcataaatgt ggtcgcctgc acgggcattc ctttatggtg cgactggaaa ttaccgggga
     5221 agtcgatccg catacgggct ggattatcga tttcgctgaa ctgaaagcgg cgtttaagcc
     5281 aacttatgag cgcctcgatc accattatct caatgatatt ccaggtctgg aaaacccaac
     5341 cagcgaagtt ttagcaaaat ggatttggga tcaggttaaa cccgttgtgc cgctgttaag
     5401 tgcggtgatg gtaaaagaaa cctgcaccgc agggtgtatc tatcgcggcg aatgataaga
     5461 gtgtgtcggc ggtcaatttc ccttaagtaa cgctatgtta gggtgttgtg ttctggatat
     5521 ctggggcatg acatggaaga cgactgcgac attattatta ttggtgccgg tattgcaggc
     5581 accgcttgcg cgttacgctg cgcgcgagcg ggtttatccg ttttgttact ggaacgcgct
     5641 gaaatccccg gcagcaaaaa tctttccggc gggcggttat atacccatgc actcgcggaa
     5701 ctcctcccgc aatttcatct gaccgcgcct cttgaacgac gcatcactca cgaaagcctt
     5761 tccctgttaa cgcccgatgg cgtaacgacg ttttccagct tacagcccgg cggtgaatcc
     5821 tggagtgtat tacgtgcacg gttcgatccg tggctggttg ccgaagccga aaaagaaggt
     5881 gtcgaatgca tccccggagc gacggtggat gcactgtatg aagaaaacgg cagagtctgt
     5941 ggcgttattt gtggtgacga tattctccgc gcccgttatg tggtgctggc agaaggtgcc
     6001 aacagcgtcc tggctgaacg tcacgggtta gtgactcgtc ctgctggcga agcgatggcg
     6061 ttggggatca aagaagtgct gtcgctggaa acatccgcta ttgaagaacg ttttcatctg
     6121 gagaataacg aaggcgcagc gttgctgttc agcggcggaa tctgtgatga cttacccggc
     6181 ggcgcatttc tttatactaa tcaacaaacg ctctcgttag ggattgtttg cccgctctct
     6241 tcccttacgc aaagtcgtgt tccggcaagc gagctgctga ctcgctttaa agcgcatccg
     6301 gcagtgcgcc cgcttatcaa aaacacggaa tcactggagt atggtgcgca tctggagcca
     6361 gaaggtggct tgcacagtat gccggtgcaa tacgccggta acggctggct gctggtgggc
     6421 gatgcgttgc gcagttgcgt caataccgga atttccgtgc gcggcatgga tatggcgctg
     6481 actggcgcac aggcggcggc acaaacgcta ataagcgcct gccagcaccg cgagccgcaa
     6541 aatctgtttg cgctttatca tcacaacgta gagcgcagcc tgctatggga tgttctacaa
     6601 cgttatcagc atgttccggc gcttttgcaa cgccctggct ggtatcgggc gtggcctgcg
     6661 ttaatgcagg atatttcccg cgatttatgg gatcagggtg ataaacctgt tccaccgctg
     6721 cgccagttat tctggcgtca tttacgtcgt catggcctgt ggcatctggc gggcgatgtt
     6781 atcaggagtc tgcgatgtct gtagcccata atctctggcg cgttgctgat gcgccgcaca
     6841 ttgttccggc tgactccgtt gagcgtcaga tggtacaacg gttgattaac gcctgtccgg
     6901 caggtctttt ttcgctcaca ccggaaggtg acttacgcgt tgattatcac ggctgcctgg
     6961 agtgtggcac ctgccgtttg ctgtgcgacg aatccacact acaacagtgg cgctatccgc
     7021 cttccggatt cggcatcacc taccgctttg gataaataat aaggataatt tatgcccctc
     7081 ttacacctac tccgccagaa tccggtgatt gctgccgtta aagataatgc cagcctgcaa
     7141 ctggctatcg attctgaatg ccagtttatt tccgtgttgt acggcaatat ctgcaccatc
     7201 agtaatatcg ttaaaaagat taagaacgcc gggaaatatg cttttattca tgttgatttg
     7261 ctggaaggcg cgtcaaataa agaagtcgtt attcagtttt tgaaactggt gaccgaggcg
     7321 gacggcatta tcagtaccaa agcctcaatg ttgaaagcgg caagagcgga aggttttttc
     7381 tgtattcatc gcctgtttat tgttgattca atttcgtttc acaacattga taagcaagtt
     7441 gcgcaatcga atccggattg tattgagatc ctgccaggct gtatgcccaa agtgctgggc
     7501 tgggtgacag agaaaatccg ccaaccgctg attgccggtg ggctggtgtg cgatgaagaa
     7561 gatgcgcgta atgcgattaa cgcgggtgtc gtggcgcttt ccaccacgaa taccggggtc
     7621 tggacgttag cgaaaaaatt actttgacgg gataatcgta accaattgaa tttggtttga
     7681 tttgtaggcc gcacgccaca tccgacattc agcgcctgat gcgacgcttg acgcgtctta
     7741 tcaggcctac aagtccccag gccagaaccg taggcaggat catccggcaa cacgttattg
     7801 gcaatctgcg tgaatgtttg tcaccagtgc ctccagcacc actttccagt catcaaccac
     7861 gccaacatct gcctgtgaaa ataccgcagc gctggcgtca tgattaatcg ccaccacaaa
     7921 cttgctattg cgtacgcccg ccattaacgc tgctgcgcca gaagcgccaa caacaataca
     7981 cacctcaggt gccagcagat gtccggaaat accaatcact ttttcagcat caacgccacc
     8041 gttcatcact cgcgcccgac tgtagcccac ttcggctccc agcttttctg ccagcatagc
     8101 gatttcctgg ttatctgcct ctccgccctg ccccaccacc agcacccgtc tggcctcagc
     8161 taatgggtcg cgagtaacat ttttcagatc ctctgtgcta accagccagt ccggaagcgc
     8221 acccgggaca atgttcagtt gttgcattcc agagggcaac gtggcgtttt tcgctgctcc
     8281 cgcctgacgc gccagggaaa ggcatagcgg gcgtttttct gtttgcagcg tcgccgttag
     8341 cgcatttccc cagtgggatt tgcgcacgct tacggttgga atatctaacg aagtcacctg
     8401 gcagatgctt gcaccatgta aacgccaggc cagccgggtc gacagttcat cgccaaacgt
     8461 tcccggcgga aagagaacaa cgtccgcagg cgttcgctgc cactgttcca ccaacgcatc
     8521 cagcacctgt tccgccacta caggctgagg ttcaatctgc cagtgcgcca gggtacaacc
     8581 gctgaaatcc tgcgccgcca gccagcttgc tattgcggca ttctcctgat ttattgtcac
     8641 aattgcgata ttcatggctg catcctttgg cgtaaatagt cctgccatag cttttgagct
     8701 ttttctgcta ccgtttgccc atcaatcagc gtcgctccgc gtcgttgctc tgctcttgcc
     8761 agttgcagac actgcatcgc tggcatttcg gcagcaacag ttttacggat aatttctgct
     8821 ttccccgcag ccattcgctg gcgcattccc ggtactggca aggccacttc accgcactga
     8881 cgcacggcaa tcaccgcagg caagcgaacc cggcaacaac gcaacccatg ttcagtacgc
     8941 tgttcgaggg tgataaacag cgcgtcgaga gtgaaacgct ccacctgggt gaagcagggc
     9001 cagcccagca tttccgccag caaaaatggc gtttgcccat tctgcccttc gctgctttgg
     9061 cagccggtaa ttatcagatc cagcggattc tgatgctgcc attcggcaat atggcgagcg
     9121 acaaattccg gcgcaaagcg cagatctgcc gccgtctcca gcaatacagc ttcctcaaac
     9181 ccaagagcca tgagatagcg cagccagtgc aatgcccgtt catcccccat gctcaacgcg
     9241 gttaaagaca tcggcgtacc gttttttctc tgcgccaaca gcagcgcggc agcggcctgt
     9301 tcatcagcac cgagtaaact tcgcagtagc gaaatatccg gtccgctttt accctgagcc
     9361 gccgcctgcc actctttttc cgccagcatt ccggcatccg gttcggcttt aaacgctaac
     9421 agaatgttca ttcgcacctc ctacatttcc cgccgccacc agcgggaggg ctttagtttc
     9481 tggtgcccat agccaggtca caacaaaatc aaccaacaac actgtcgcca ggagcaataa
     9541 ggtgacttgc attccccact gcgccagcac ccacggcagc aggccagtac ttaccgccgc
     9601 gccaagtcga ctcatggcgg tggcaaaacc gacgcccagc gagcgaatgt cagtaggaaa
     9661 actttccgca ggcaaaatgc ccaccagatt actgactgcc gaaatggtgg tgctgaagag
     9721 aacaaaaagc agcagcgtta atgaactgcc ggaaggcaaa caggccatca ctaccagcgt
     9781 tgccgccagc agcaaaaaac ttcccagcaa aaatttgcga tgtgccagca ggtgcgtcag
     9841 aactaatccc agcagcgcgc ccacaattaa caacgcatta agcatcaggc tggcagtcag
     9901 cgcatcttcc agaccaatag tctgggcgat agttggcagc caggtataaa tcacaaacca
     9961 tgggattacg aggcagacaa agaagacgct gttaaacgcc gtgcgccgcc agtaacgcga
    10021 agagaacaag gttttgatgt gtttatgggt cgccgttacc acttcatcgc ccagtaaaac
    10081 atggggacca aaatagcgat gcacgatagc gtgagcttct gcaaaacgcc cctggcgcag
    10141 tagccagcgt ggtgattctg gcgttcccca gcgtaataac gtaatcaaca acgcgggcag
    10201 agctgccgat gccagtagcc agcgccaggc ctccgggttt tcggaaataa agtgatgtcc
    10261 ggcaatactt gccagcacat agcctacggt ccacaccacg ctgaatgcgc ccagcaaaat
    10321 accgcgatgg cggcgcgggg aaaattcagc cagcaaggtt aatgccatta ggatcgaact
    10381 gctttttcag cccttccagc aacgcccacg cgctgccgtg ttccagtttg ctccagtgaa
    10441 cgcgatgttt accgataccg tggtggtgca ccatcgaacc gccgaggcga atggtttctt
    10501 cacagatgat cttgttgagc ggattgtggt acttgtcgat ttcctcttcc ggcttacagt
    10561 caacgacgtt gtaatcgtag acgaagtaca tgttggtgcc gttctgatag ctatgagagg
    10621 aatgaccgcc cagcatggtg atgtcgtcgg cgtgcgggaa ctcagtacga atacggttaa
    10681 taacgctttc gtagatttcg tggatgcagc tccagcagcc ggacacttca gtggtaaagc
    10741 ccatgttgcc ggttttgagg atctgcacac gttcggcagc cactttatcc ggtccccagt
    10801 tcaggttgtt aaaccaggtt tcgatcagct tgctgtccac gcgctggcat tgcgggtagc
    10861 gggcaacgat ttccgcaatc ccttcgcccg tcgccttcgc aatgcgaggg ttaccttcag
    10921 ccataaagat cagcacgcat tttccgtctg caaaatgggt gaagtgttgg gtgccatctt
    10981 cagcgtcata caaacgagcg atcgacggac gatacccttc caccatgact tcacgcagga
    11041 tgttgaagtc ggttttcatg tcttccagga tatagccgta gaagaggttg ttttccgggg
    11101 tgaatttaaa gattttcact gttacttcag tgatatagca aagtgcacct tcgttaccga
    11161 tgatgatgtg gcgaatgtcc gggccagccg cgcggcgtgg cacgttttta atgcgtgtga
    11221 cggtgccatc agccaatact gcttccagac caacgaccat atcttcgatt gcgccgtaga
    11281 gtgtggagaa ctgcccgata ctgcgggttg ctaccaagcc gcccatctgc gccagcggct
    11341 ttgactgcgg agaatgcccc gtggtgtaac ctttttcacg caacgcattt tccagcacct
    11401 ccagcggaac gccacattgc gccgtcgcct gcatattctc aatatcaata ttaatgattt
    11461 gattcatggc ggagccgtca agcaccaccg agttttccac aacggtttcc agcccacctt
    11521 cagtggcgga agcgccggta cgcggcacac cgttaatttt gtgcgcattc ataaaattca
    11581 gcacacggga tacttgctct gtggaaccga gttttacgac cgctgccgga atcggcaaag
    11641 tataaatgcc atgaatatcc ggaaatttac gaaaacggtc aatactgttc ttctttaata
    11701 ctgtttcatc ggtaattacg cgatctgcac caacaatttc ctttagttgg tcgacaatcg
    11761 ctgcgcgaga taaagacata gtaattcctt cctgataata aaaatggaat aatcattaat
    11821 aagagaaaac agaaaccacc gttatggatt agcgcactaa ataaccacca ttgaccacta
    11881 ataaatgccc attgacataa tttgaagccg gacttgcgag gaatacagct gcgcccatta
    11941 aatcctgagt atcgccccaa cggtttgccg gaatatgatc aagaacgcgc tgattggttt
    12001 ctggattact gcgtgtcgcc agcgtaatat ctgttgcata atagccagga gcaataccat
    12061 ttacctgaat attatattga cctagttcat cacaataagc tttggtgaat ccggcaagag
    12121 catgtttagt ggcagaatat gcaggtgacc attgtccacc taagtaagag aacaatgaac
    12181 agatattaat aattttgccg cttttctgcg ggatcataat ttttgcagct tcatagctta
    12241 actcgaatgc ggcggtcagg ttcacatcaa tcatcggatc ccagtcggca cgaccgaagt
    12301 ccagcacctt attcagctta caaataccgg cattgtttac cagaatatca actgtaccga
    12361 aacgctcaca gcaagcagcg ataatcttct gcggcgcgcc ttctgcggtg atatccacct
    12421 gcatgaagtc cacctcaaca ccctgttttt caaacatttc ctttgtttcg ccgttatctt
    12481 tcaatcattt cctttgtttc gccgttatct ttgacgaaac taggaataaa gatatttgcg
    12541 ccagctttgg ccaacgccat ggcaaatgcc tggcctaaac cgctattccc accggtaaca
    12601 attgcggttt tacctttcag tgagaaaaaa tccattgaga acgcattgag agattcgatt
    12661 gacatagtta gctccaattt ccgtaaggca aaaaaaagaa aggtaacttc ccccgcgaga
    12721 gggaagttac ctttctcatt atctctggta actggagata atttttagca taacggcata
    12781 actgctaatg tgatcgtaat cacagtgtga tattcgtatt gtcaaatagt tttaaaaagc
    12841 agaagcgtga ttactctcac acaacacctc tgctttccat gttatttctt attgcaatta
    12901 ctagagacct gagaaaagta atccacctga aagggc
//
LOCUS       DAZRUF010000116        12853 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZRUF010000116 DAZRUF010000000
VERSION     DAZRUF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.116"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..180
                     /locus_tag="ACHXOL_002569"
     CDS             <1..180
                     /locus_tag="ACHXOL_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809524.1"
                     /translation="SLTTTLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPV
                     TNIVLFLILIFFKKR"
     gene            complement(227..1114)
                     /gene="rarD"
                     /locus_tag="ACHXOL_002570"
     CDS             complement(227..1114)
                     /gene="rarD"
                     /locus_tag="ACHXOL_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR6809525.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1166..1633)
                     /gene="yigI"
                     /locus_tag="ACHXOL_002571"
     CDS             complement(1166..1633)
                     /gene="yigI"
                     /locus_tag="ACHXOL_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR6809526.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1798..2667
                     /gene="pldA"
                     /locus_tag="ACHXOL_002572"
     CDS             1798..2667
                     /gene="pldA"
                     /locus_tag="ACHXOL_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR6809527.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2800..4629
                     /gene="recQ"
                     /locus_tag="ACHXOL_002573"
     CDS             2800..4629
                     /gene="recQ"
                     /locus_tag="ACHXOL_002573"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR6809528.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4693..5310
                     /gene="rhtC"
                     /locus_tag="ACHXOL_002574"
     CDS             4693..5310
                     /gene="rhtC"
                     /locus_tag="ACHXOL_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR6809529.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRTRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5372..5992)
                     /gene="rhtB"
                     /locus_tag="ACHXOL_002575"
     CDS             complement(5372..5992)
                     /gene="rhtB"
                     /locus_tag="ACHXOL_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR6809530.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6103..7125
                     /gene="pldB"
                     /locus_tag="ACHXOL_002576"
     CDS             6103..7125
                     /gene="pldB"
                     /locus_tag="ACHXOL_002576"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR6809531.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7133..7933
                     /gene="yigL"
                     /locus_tag="ACHXOL_002577"
     CDS             7133..7933
                     /gene="yigL"
                     /locus_tag="ACHXOL_002577"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR6809532.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8009..8908
                     /gene="bioP"
                     /locus_tag="ACHXOL_002578"
     CDS             8009..8908
                     /gene="bioP"
                     /locus_tag="ACHXOL_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR6809533.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8796..9749)
                     /gene="metR"
                     /locus_tag="ACHXOL_002579"
     CDS             complement(8796..9749)
                     /gene="metR"
                     /locus_tag="ACHXOL_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR6809534.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9985..12246
                     /gene="metE"
                     /locus_tag="ACHXOL_002580"
     CDS             9985..12246
                     /gene="metE"
                     /locus_tag="ACHXOL_002580"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR6809535.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12368..12490)
                     /locus_tag="ACHXOL_002581"
     CDS             complement(12368..12490)
                     /locus_tag="ACHXOL_002581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809536.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12531..12776)
                     /locus_tag="ACHXOL_002582"
     CDS             complement(12531..12776)
                     /locus_tag="ACHXOL_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR6809537.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3029 a         3232 c         3441 g         3151 t
ORIGIN      
        1 agcctgacta cgacattaaa tttaagaagt ataggtatag ggttaagttt tgggttttgt
       61 caaatccttg gtagatatac gatgtctcta ggatggagta agtactgggg agttttgggg
      121 gtatttcctg taacaaatat tgttcttttt cttatcttaa tttttttcaa aaaaagataa
      181 ataagtcagt gggtaggctc tttaaacggc ctacccatgt taattcttaa ctttttctac
      241 gctgcgtata aatcgcatcc atcacaaaaa ttgccagcgc cacccaaata aaggcgaaag
      301 tcaccatctt atcggcaccc ggtttttcac cataaaacgt cacagccagc aggaacatca
      361 gcgtcgggcc aatgtactgg aaaaagccta acgttgagag acgcaagcgc gtggcagcgg
      421 cggtaaaaca caacagcggt acggtagtga caataccggc ggcgatcagc agtaaattca
      481 gcgacatcgg gttttgcccc atatggctgg ttgagctgtc ggcaatagca aacaggtaaa
      541 ttgccgccac gggcagcagc cacatggttt cgattaacat gccggtttgc gcttcaacgg
      601 caatcttctt gcgtaccaga ccgtagaagg caaaactaaa tgccagtccc agcgcgataa
      661 taggtagcga accaaaagtc cacagctgga ctaacacgcc acatatcgcc agaatcaccg
      721 ccagccattg catccggcgg aatcgctcgc cgaggaaaat catccccagc acaatgttca
      781 ccagcgggtt aataaagtaa ccaaggctcg cttccagcat atggtgattg ttcaccgccc
      841 agataaacag tagccagttg ccaccaatca gcacggcaga gactgccagc ataaaaattt
      901 tctgtggcgt ctgaatcagc gtttttaaat aggaccactg gcggcaaatg ctcatcagca
      961 ccaccataaa gaaaaacgac cagatcacgc gatgcgtcag gatttcatcg gcgggcacgt
     1021 agtaaatcaa cttgaagtac gctggcgcta taccccaaat aaaataagcg gcaagagcga
     1081 gtaatacgcc ctgccgcgtt tgttttgcat ccatcgggaa tactcatttt taattggtaa
     1141 cagcagttta cctgctttta tgtcttcaac ctaccatata ggtggcggtg gcactggcaa
     1201 tataaagctg ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta ttgcctgcac
     1261 gcaacaggct actggtagca gtaaaacgct cgcccctgcc tgggcgcaga taatcaacgc
     1321 gaagatcaat ggtccccatc cgcgatagcc gctggcgtag ttcatcttca ctgatggttt
     1381 cgtggcgggt taaggtactt cccacgcaca ccagaccggc ggcgacatcc agcgccgacg
     1441 caatgacccc gccgtgcaaa atgctttgcg cccagttgcc caccatcatt ggctgatttt
     1501 taaaggccag ctgtgcgaac tctttttcgt aacgctccag ttccatcccc aatgcgcggt
     1561 taaatggcat gtgataaaca aacatctcac ccactaattt cagggcttgt tcagcggtca
     1621 gtacggcaga catatcatcc ttacacttca ttggttaatg aaatgttgat tttatgcttc
     1681 tttgttgttg gtttctactt taggaaggga taactaacgg ctatggagtt aagtatgtaa
     1741 aatagcccgc agaaaaatat tcaccttatc aataattcgt tacggagaac acgaccgatg
     1801 cggactctgc agggctggtt gttgccggtg tttatgttgc ctatggcagt atatgcacaa
     1861 gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat tatcgccaat
     1921 atgctgcagg agcatgacaa tccgttcacg ctctatcctt atgacaccaa ctacctcatt
     1981 tacacccaaa ccagcgatct gaataaagaa gcgattgcca gttacgactg ggcggaaaat
     2041 gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat ttccgctgtg gcgtgggatt
     2101 ttaggcccga actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg gcaactgtcc
     2161 aatagcgaag agtcttcacc gtttcgtgaa accaactacg aaccgcaatt gttcctcggt
     2221 tttgccaccg attactgttt tgcaggttgg acgctgcgcg atgtggagat ggggtataac
     2281 cacgactcta acgggcgttc cgacccgacc tcccgcagct ggaaccgcct ttatactcgc
     2341 ctgatggcag aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt ggtgggtaat
     2401 actgacgata acccggatat caccaaatat atgggttact accagcttaa aatcggctat
     2461 cacctcggtg atgcggtgtt gagtgcgaaa ggacagtaca actggaatac cggttacggc
     2521 ggcgcggagt taggcttaag ttacccgatc accaaacatg tgcgccttta tactcaggtt
     2581 tacagcggct atggcgaatc gctcatcgac tataacttca accagacccg tgtcggtgta
     2641 ggagttatgc taaacgactt gttttgatga acggttgagt ggttggcaaa tctggaaccc
     2701 agcatccagg attaccctct cagagactaa aagcattgca gtttctcgcg caggcgctga
     2761 aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc ggaagtgttg
     2821 aatctggagt ccggagctaa acaggtttta caagaaacct ttggctacca acagtttcgc
     2881 cccggccagg aagaaattat cgacactgtg ctttccggtc gcgattgcct ggtcgtcatg
     2941 cccaccggtg gcggaaaatc cctttgctat caaattcctg ccttattgct aaacggcctt
     3001 accgtggttg tttcaccgct gatttcgttg atgaaagatc aggtggatca actgcaagcc
     3061 aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc gcgagcagca acttgaagtg
     3121 atgacaggct gccgcaccgg gcaaattcgt ctgctttata tcgccccgga acgcctgatg
     3181 ctggataact ttcttgagca tctggcgcac tggaatccgg tgttattagc cgttgatgaa
     3241 gcgcactgta tctcccaatg gggccacgat ttccgcccgg aatatgccgc gcttggtcag
     3301 ttacgccagc gtttcccgac cctgccgttt atggcgctga ctgccacagc cgacgacacc
     3361 acgcgccagg atatcgtgcg cttgctgggg ctgaacgatc cgctgattca aatcagcagt
     3421 tttgaccgtc cgaatattcg ctacatgctg atggagaaat tcaaaccgct cgatcagttg
     3481 atgcgctacg tgcaggaaca gcgcggtaag tccggcatta tctactgcaa cagccgggcg
     3541 aaagtagaag acaccgctgc gcgcctgcaa agcaagggta ttagcgcggc ggcctatcat
     3601 gccgggctgg aaaataatgt ccgcgccgac gtgcaggaga aattccagcg cgatgacctg
     3661 caaattgtgg tggcgacggt ggcgttcggc atgggcatca ataaaccaaa cgttcgcttc
     3721 gtggtccact ttgatattcc gcgcaatatc gaatcctatt atcaggaaac cggtcgcgcc
     3781 gggcgtgatg gcctgcctgc ggaagcgatg ctgttttacg atccggctga tatggcgtgg
     3841 ctgcgccgtt gtctggaaga gaagccgcag gggcagttgc aggatattga gcgccacaaa
     3901 ctcaatgcga tgggcgcgtt tgccgaagcg caaacttgcc gtcgtctggt attgctgaac
     3961 tattttggcg aagggcgtca ggagccgtgc gggaactgcg atatctgcct cgatccgccg
     4021 aaacagtacg acggttcaac cgatgctcag attgcccttt ccaccattgg tcgtgtgaat
     4081 cagcggtttg ggatgggtta tgtggtggaa gtgattcgtg gtgctaataa ccagcgtatc
     4141 cgcgactatg gtcatgacaa actgaaagtc tatggcatgg gccgtgataa aagccatgaa
     4201 cattgggtga gcgtgatccg ccagctgatt cacctcggcc tggtgacgca aaatattgcc
     4261 cagcattctg ccctacaact gacagaggcc gcgcgcccgg tgctgcgcgg cgaatcctct
     4321 ttgcaacttg ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca gaaatcgttc
     4381 ggcggcaact atgatcgcaa actgttcgcc aaattacgca aactgcgtaa atcgattgcc
     4441 gatgaaagta atgtcccgcc gtacgtggtg tttaacgacg caaccttgat tgagatggct
     4501 gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg gatgcgcaag
     4561 ctggaacgct ttggtaaacc gtttatggcg ctgattcgtg cgcatgtcga tggcgacgac
     4621 gaagagtagt cagcagcata aaaaagtgcc aggatgaaga ctccgtaaac gtttcccccg
     4681 cgagtcaaat gtatgttgat gttatttctc accgtcgcca tggtgcacat tgtggcgctt
     4741 atgagccccg gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg tacccgtaaa
     4801 gaagcgatga tgggcgtgct gggcattacc tgcggtgtaa tggtttgggc tgggattgcg
     4861 ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc tgcatacgct tatggttggc
     4921 ggtggcctgt atctctgctg gatgggttac cagatgctgc gcggcgcgct gaaaaaagag
     4981 gcggtttctg cacctgcgcc acaggtcgag ctggcgaaaa gtgggcgcag tttcctgaaa
     5041 ggtttactga ccaatctcgc taatccgaaa gcgattatct actttggctc ggtgttctca
     5101 ttgtttgtcg gtgataacgt tggcactacc gcgcgctggg gcatttttgc gctgatcatt
     5161 gtcgaaacgc tggcgtggtt tatcgtcgtt gccagcctgt ttgccctgcc gcaaatgcgc
     5221 cgtggttatc aacgtctggc gaagtggatt gatggttttg ccggggcgtt atttgccgga
     5281 tttggcattc atttgattat ttcgcggtga tgccagacgc gtcttcagag taagtcggat
     5341 aaggcgttta cgccgcatcc gacattattt ttcacgcatg cctcgccgat gctaacagcg
     5401 ctcccaccag cataaacaac gagccgaaaa tcttattcag cgccttcatc tgctttggtc
     5461 ctttaatcca tagagcaatc cgttgagcaa gggtggcgta accgatcatc acaataatat
     5521 cgaccacaat agtggtgacg ccgagcacga tatactgcat cagttgcggc tgttgcggca
     5581 tgatgaattg cggaaatagc gccgccagaa acacaatact tttgggattg gtgagattca
     5641 caaaaactgc gcgctggaac aaatgtcgac gcgattgagt agaggccagc gatttaaggt
     5701 caattgcacc agcggcgcgc cactgctgga ttcccagcca aatcaagtaa gccgcgcctg
     5761 cccacttcaa cacttcaaac gcaatcactg agcgggaaaa tagcgtcccc aaccccacgc
     5821 caaccagcac aatatgaatc gccagtccgg tctgaagccc agcaatagac gccaccgcgc
     5881 cgcgataacc gtggttgagc gaggtggtca tagtgttgat tgcaccagag cctggcgaca
     5941 ggcttaaaat gatcgatgtc agcaggtagg caaaccacca ttctaaggtc atgatgaact
     6001 cccggtgtgt ctatttttgt gccacaatac gctactgtcg cagcgttgtg tcaggcacgc
     6061 taaaaaaaac gattttacgt ggtttaagag gcagattacc cgatgtttca gcagcaaaaa
     6121 gactgggaaa caagagaaaa cgcgtttgct gcttttacca tgggaccgct gactgatttc
     6181 tggcgtcagc gtgatgaagc agagtttact ggtgtggatg acattccggt gcgctttgtc
     6241 cgttttcgcg cacagcacca tgaccgggtg gtagtcatct gcccggggcg tattgagagc
     6301 tacgtaaaat atgcggaact ggcctatgac ctgttccatt tggggtttga tgtcttaatc
     6361 atcgaccatc gcgggcaggg acgttccggt cgcctgttag ccgatccgca tctcgggcat
     6421 gttaatcgct ttaatgatta tgttgatgat ctggcggcat tctggcagca ggaggttcag
     6481 cccggtccgt ggcgtaaacg ctatatactg gcacattcga tgggcggtgc gatctccaca
     6541 ttatttctgc aacgccatcc aggtgtatgt gacgccattg cgctaactgc gccaatgttt
     6601 gggatcgtga ttcgtatgcc gtcatttatg gcacggcaga tcctcaactg ggccgaagcg
     6661 catccacgtt tccgtgatgg ctatgcaata ggcaccgggc gctggcgcgc gttgccgttt
     6721 gctatcaacg tactgaccca cagcagacag cgatatcgac gtaacttacg cttctatgct
     6781 gatgacccaa cgattcgcgt cggtgggccg acctaccatt gggtacgcga aagtattctg
     6841 gctggcgaac aggtgttagc cggtgcgggt gatgacgcca cgccaacgct tctcttgcag
     6901 gctgaagagg aacgcgtggt ggataaccgc atgcatgacc gtttttgtga actccgcacc
     6961 gccgcgggcc atcctgtcga aggaggacga ccgttggtaa ttaaaggtgc ttaccatgag
     7021 atcctttttg aaaaggacgc aatgcgctca gtcgcgctcc acgccattgt tgattttttc
     7081 aacaggcata actcacccag cggaaaccgc tctacagagg tttaaatttc ttatgtacca
     7141 ggttgttgcg tctgatttag atggcacgtt actttctccc gaccatacgt tatcccctta
     7201 cgccaaagaa accctgaagc tgctcaccgc gcgcggcatc aactttgtgt ttgcgaccgg
     7261 tcgtcaccac gttgatgtgg ggcaaattcg cgataatctg gagatcaagt cgtacatgat
     7321 cacctccaac ggcgcgcgcg ttcacgatct ggatggcaat ctgatttttg ctcataacct
     7381 ggatcgcgac attgccagcg atctgtttgg cgtagtcaac gacaatctgg acatcattac
     7441 taacgtttat cgcgacgacg aatggtttat gaatcgccat cgcccggaag agatgcgctt
     7501 ttttaaagaa gcggtgttca aatatgcgct gtatgagcct ggattactgg agccggaagg
     7561 cgtcagcaaa gtgttcttca cctgcgattc ccatgaacaa ctgctgccgc tggagcaggc
     7621 gattaacgct cgttggggcg atcgcgtcaa cgttagtttc tctaccttaa cctgtctgga
     7681 agtgatggcg ggcggcgttt caaaaggcca tgcgctggaa gcggtggcga agaaactggg
     7741 ctacagcctg aaggattgta ttgcgtttgg tgacgggatg aacgatgccg aaatgctgtc
     7801 gatggcgggg aaaggctgca ttatgggcag tgcgcaccag cgtctgaaag accttcatcc
     7861 cgagctggaa gtgattggta ctaatgccga cgacgcggtg ccgcattatc tgcgtaaact
     7921 ctatttatcg taatcgttct ttatttggtc agttgtcaac ctgatacttc gctacaatgg
     7981 atactcgtta atcaaagagt tttccattgt ggcgctactt atcatcacca cgattctgtg
     8041 ggccttctcc tttagctttt atggcgagta ccttgcgggg cacgtcgata gctattttgc
     8101 ggtgctggtg cgcgttggcc tggcggcact cgtttttctg ccgtttctgc gtacccgtgg
     8161 caatagcctg aaaacggtcg gcctgtatat gctggtgggc gcgatgcagc tcggcgtgat
     8221 gtatatgctg agtttccgcg cttatctcta cctgacggtc tccgaattgt tgctgttcac
     8281 cgtactgacg ccgctctaca tcacgctgat ttatgacatc atgagtaagc gccgtctgcg
     8341 ctggggctat gcttttagcg ccttgctggc ggtgattggt gccgggatta ttcgctatga
     8401 tcaggttacc gaccatttct ggactggctt gttgctggtg caactctcca atatcacttt
     8461 tgccatcggc atggtgggtt acaaacgcct gatggaaact cgcccgatgc cgcagcataa
     8521 cgcctttgcg tggttctatc ttggcgcgtt cctggtggcg gtaattgcat ggttcttgct
     8581 gggaaatgcg cagaaaatgc cgcaaaccac gttgcaatgg ggcattctgg tgtttcttgg
     8641 cgtggtggct tccgggattg gctactttat gtggaactac ggcgcgacgc aggtggacgc
     8701 cggaacgctg ggcattatga ataatatgca cgttccggca gggctgctgg taaacctggc
     8761 tatctggcac caacagccgc actggccaac gttcattaca ggcgcgctgg tgatcctggc
     8821 ctcactgtgg gtgcatcgta agtgggtcgc tccgcgctct tcacaaacgg cagatgatcg
     8881 caggcgtgat tgcgcgctga gcgaataaat gcttctgtaa tcggctgacg ctgctcgcca
     8941 tcgcgcacgg cggcgtacag tcggctccac aagccttcgc ccagggtttt ggtcaccacc
     9001 agaccctggc gctcaaaact ctctactacc caatgcggta gcgcggcaat acccatccgc
     9061 gcggcaacca tctgaatcaa caataaggtg ttatcgacgc ttttcagtga cgggctgacg
     9121 cctgccggct gaagaaaatg ccgccagaca tccagtcgac tacgctgcac cggataaatc
     9181 aacagcgtct cgctggcgag atcttccggt gtaattcgcg ttttcgccgc cagtggatgg
     9241 tcaggtgcta acaccagacg cacttcatag tcgaacatcg gcgaataatg caggccactg
     9301 cgcggcagga tatcggaggt catcaccaga tccagctcgc cctgttgcaa cgcaggctgc
     9361 gggtcaaacg tcacgcccga tttaaaatcc atctctacct gcggccagtt cttatggaaa
     9421 ttttctaacg cgggcgtcag ccactgaata cagctatggc actcaatggc aatgcgcaga
     9481 cgcgtctgct gcggttcatt gcaggcttgc agtgcctggc tgatttgcgg cagcacctgg
     9541 tttgccagtt gcaacaggat ttctccctgc ggtgtaaagc gtagcggctg gctcttacgc
     9601 acaaatagcc ggaagccaag gcgttgttcc agatcgctaa actggtgaga cagggcggat
     9661 tgcgtctgat gcaacgtcgc cgcagcggct gcgagagagc cgcagttccg caacgcttgt
     9721 agcgttttca ggtgttttac ttcgatcatg aaagtccttc acttcggcat gaataatttg
     9781 cgcttgagga atatacagta accgccaatt atggatgtgt aaacatctgg acggctaaaa
     9841 tccttcgtct tttaaattta tggtgcgttg gctgcgtttc tccaccccgg tcacttactt
     9901 cagtaagctc ccggggatga ataaacttgc cgccttccct aaattcaaaa tccataggat
     9961 ttacatataa ttagaggaag aaaaatgaca atattgaatc acaccctcgg tttccctcgc
    10021 gttggcctgc gtcgcgagct gaaaaaagcg caagaaagtt attgggcggg gaactccacg
    10081 cgtgaagaac tgctgacagt agggcgtgaa ctgcgtgctc gtcactggga tcaacaaaag
    10141 caagcgggta tcgacctgct gccggtgggc gattttgcct ggtacgatca tgtactgacc
    10201 accagtttgc tgctgggtaa tgttccgcca cgtcatcaga acaaagacgg ttcggtagat
    10261 atcgacaccc tgttccgtat tggtcgtgga cgtgcgccga ctggcgaacc tgcggcggca
    10321 gcggaaatga ccaaatggtt taacaccaac tatcactaca tggtgccgga gttcgttaaa
    10381 ggccaacagt tcaaactgac ctggacgcag ctgctggagg aagtggacga ggcgctggcg
    10441 ctgggccaca acgttaagcc tgtgctgctg gggccggtta cttacctgtg gctggggaaa
    10501 gtgaaaggtg aacagtttga ccgccttagc ctgctgaacg acattctgcc ggtttatcag
    10561 caagtgctgg cagaactggc gaaacgcggc atcgagtggg tacagattga tgaacccgcg
    10621 ctggtactgg aactaccaca ggcgtggctg gacgcataca aacccgctta cgacgcgctc
    10681 cagggacagg tgaaactgct gctgaccacc tattttgaag gcgtaacgcc aaatctcgac
    10741 acgattactg cgctgcctgt tcagggtctg catgttgatc tcgtacatgg taaagatgac
    10801 gttgctgaac tgcacaagcg cctgccttct gactggttgc tgtctgcggg tctgatcaat
    10861 ggtcgtaacg tctggcgcgc cgatcttacc gagaaatatg cgcaaattaa ggacattgtc
    10921 ggcaaacgtg atttgtgggt ggcatcttcc tgctcgttgc tgcacagccc catcgacctg
    10981 agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt ttgccttcgc cctacaaaaa
    11041 tgccatgaac tagcactgct gcgcgatgcg ctgaacagtg gtgacacggc agctctggca
    11101 gagtggagcg ccccaattca ggcgcgtcgt cactctactc gtgtacataa tccggcggta
    11161 gaaaagcgtc tggcggcgat caccgcccag gacagccagc gtgcgaatgt ctatgaagtg
    11221 cgtgctgaag cccagcgtgc acgttttaaa ctgccagcgt ggccgaccac cacgattggt
    11281 tccttcccgc aaaccacgga aattcgtacc ctgcgtctgg atttcaaaaa gggcaatctc
    11341 gacgccaata actaccgcac gggcattgcg gaacatatca ggcaggccat tgttgaacag
    11401 gaacgtttgg gactggatgt gctggtacat ggcgaggccg agcgtaatga catggtggaa
    11461 tactttggcg agcacctcga cgggtttgtc tttacgcaaa acggttgggt acagagctac
    11521 ggttcccgct gcgtgaagcc accgattgtt attggtgacg ttagtcgccc ggcaccgatt
    11581 accgtggagt gggcgaagta tgcgcaatct ctgactgata aaccggtgaa agggatgctg
    11641 actggcccgg tgactattct ctgctggtcg ttcccgcgtg aagatgtcag ccgtgaaacc
    11701 atcgccaaac agattgcgct ggcgctgcgt gatgaagtgg ccgatctgga agccgctgga
    11761 attggcatca tccagattga cgaaccggct ctgcgcgaag gtttaccgct gcgtcgtagc
    11821 gactgggatg cgtatctcca gtggggcgta gaggccttcc gtatcaacgc cgccgtggcg
    11881 aaagatgaca cacaaatcca cactcacatg tgttattgcg agttcaacga catcatggat
    11941 tcgattgcgg cactggacgc agacgtcatc accatcgaaa cctcgcgttc tgacatggag
    12001 ttgctggagt cgttcgaaga gtttgattat ccgaatgaaa tcggtccagg cgtctatgac
    12061 attcactcgc caaacgtacc gagcgtggaa tggattgaag ccttgctgaa gaaagcggca
    12121 aaacgcattc cggcagagcg cctgtgggtt aacccggact gtggcctgaa aacgcgcggc
    12181 tggccagaaa cccgcgcggc actggcgaac atggtgcagg cggcgcagaa tttgcgtcgg
    12241 ggatgatgaa tattaacgag tcactgtaaa ttgcagtacg atgaaagagg caaaactgcc
    12301 tgatactagg cctacataat ttatgcaatt tattgaattt ttgggttttg ttggccggac
    12361 aaggcgttca cgccgcatcc ggcatgaaca acgcgcactt tgccaataat ctgatggctt
    12421 ccatcaccgg aagccatttt gctatctgcg cccgttcaga tacagaaata accgaatcat
    12481 ttttacgcaa aatataatcc tgagcgattt gttcagtaat caacgctacg ttatttattc
    12541 tcacttttca cgaaattgta tcccttttca gtaacgtggt agtgaggcat agacttatgt
    12601 ccgctatcct caataataac caaaccctcg ttagctaatt tgttaacaat gttaacaaca
    12661 ttaccgaatc ctggaatttg ccttatggat agtgtgcgat cgagattcat ccagttccag
    12721 tttatatctt tctcagccat aattttcagt acgttaattt cagtttcagt tatcatagtc
    12781 aagtgcatcc tggtggtata agtcattaag ttctccctta agtttataat cttctagagt
    12841 agcagtatgc gta
//
LOCUS       DAZRUF010000117        12811 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZRUF010000117 DAZRUF010000000
VERSION     DAZRUF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.117"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            93..497
                     /locus_tag="ACHXOL_002583"
     CDS             93..497
                     /locus_tag="ACHXOL_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR6809538.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            523..1236
                     /gene="msyB"
                     /locus_tag="ACHXOL_002584"
     CDS             523..1236
                     /gene="msyB"
                     /locus_tag="ACHXOL_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR6809539.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            1385..1951
                     /gene="satP"
                     /locus_tag="ACHXOL_002585"
     CDS             1385..1951
                     /gene="satP"
                     /locus_tag="ACHXOL_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR6809540.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(1986..2573)
                     /gene="mog"
                     /locus_tag="ACHXOL_002586"
     CDS             complement(1986..2573)
                     /gene="mog"
                     /locus_tag="ACHXOL_002586"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR6809541.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2688..3641)
                     /gene="tal"
                     /locus_tag="ACHXOL_002587"
     CDS             complement(2688..3641)
                     /gene="tal"
                     /locus_tag="ACHXOL_002587"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR6809542.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3920..5349
                     /locus_tag="ACHXOL_002588"
                     /pseudo
     CDS             3920..5349
                     /locus_tag="ACHXOL_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            5419..6195
                     /gene="yaaA"
                     /locus_tag="ACHXOL_002589"
     CDS             5419..6195
                     /gene="yaaA"
                     /locus_tag="ACHXOL_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR6809543.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(6348..6644)
                     /locus_tag="ACHXOL_002590"
     CDS             complement(6348..6644)
                     /locus_tag="ACHXOL_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR6809544.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(6858..8144)
                     /gene="thrC"
                     /locus_tag="ACHXOL_002591"
     CDS             complement(6858..8144)
                     /gene="thrC"
                     /locus_tag="ACHXOL_002591"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR6809545.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(8145..9077)
                     /gene="thrB"
                     /locus_tag="ACHXOL_002592"
     CDS             complement(8145..9077)
                     /gene="thrB"
                     /locus_tag="ACHXOL_002592"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR6809546.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(9079..11541)
                     /gene="thrA"
                     /locus_tag="ACHXOL_002593"
     CDS             complement(9079..11541)
                     /gene="thrA"
                     /locus_tag="ACHXOL_002593"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR6809547.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKTRDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(11568..11687)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(11623..11688)
                     /gene="thrL"
                     /locus_tag="ACHXOL_002594"
     CDS             complement(11623..11688)
                     /gene="thrL"
                     /locus_tag="ACHXOL_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR6809548.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(11902..12588)
                     /locus_tag="ACHXOL_002595"
     CDS             complement(11902..12588)
                     /locus_tag="ACHXOL_002595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR6809549.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3026 a         3383 c         3169 g         3233 t
ORIGIN      
        1 aaaagaaaag attacgtacc tgaatcttct ctttatcagc agtaaactag tgggtattca
       61 tccctctacc tcttcccact aagagaatcc ttatgaaatc cgtttttacg atttccgcca
      121 gcctggcgat tagcctgatg ctgtgctgca cggcgcaggc aaacgaccat aaactcctcg
      181 gcgtcattgc aatgccgcgt aacgaaacca acgatctggc gctgaaactt cctgtttgtc
      241 gcattgtgaa acgcatacaa ctctctgccg accatggcga tttacagtta agcggtgcat
      301 cggtttattt caaagccgcc cgtagcgcca gtcagagtct gaatattcct tcagaaataa
      361 aagaagggca aaccactgac tggatcaaca ttaacagtga taacgacaat aaacgctgcg
      421 tctcaaaaat caccttttcc ggtcatacgg tgaactcatc ggatatggcc acgctgaaaa
      481 ttatcggcga cgactaacgc cagaatatag aaaccacaaa aaatgaatgt taattacctg
      541 aatgattcag atctggattt tcttcagcat tgtagtgagg aacagttggc aaatttcgcc
      601 cgattgctca cccataatga aaaaggcaaa actcgcctct ccagcgtact gatgcgcaac
      661 gaactgttta aatcgatgga agggcatccc gagcaacatc gccgcaactg gcagctgatt
      721 gctggagaat tacagcattt tggtggcgat agtatcgcca acaaactgcg cggacacggt
      781 aaattgtatc gggccatttt gctcgatgtt tcaaaacgat tgaagctgaa agccgacaaa
      841 gagatgtcta cgtttgagat tgagcaacag ttactggaac aatttctgcg taacacctgg
      901 aagaatatgg acgaggaaca taagcaggag tttctgcacg cggtcgatgc cagggtgaat
      961 gagctggaag agctgctgcc gctgctgatg aaagacaaat tattggcaaa aggcgtgtcg
     1021 catttgcttt ccagccaatt gacccgcatt ttacgcaccc acgcagcaat gagcgtgctt
     1081 gggcatggtt tgctgcgcgg cgcgggtctg ggaggccctg tgggcgcagc attaaatggg
     1141 gttaaagcgg tcagcggcag tgcctatcgc gtgacaattc cagccgtaat acaaatcgcc
     1201 tgcctgcgcc ggatggttag cgccactcag gtctgacacc gtaaaccggg gaaaattttc
     1261 attttcccct caaaagatcg tagacactgc cccactggct gattattatg ccgcgccctg
     1321 aaaacactac tgttattcag ggaaattatt tcaccattca ttcgatgatg atttttgagg
     1381 aattatgggc aacactaagt tggctaatcc ggcaccgctg ggcctgatgg gcttcggcat
     1441 gaccaccatt ctgcttaacc tgcacaacgt gggttatttc gctctggacg gtattattct
     1501 tgccatgggc attttctacg gcggcatcgc gcaaattttt gccggtctgc tggagtacaa
     1561 aaaaggcaac actttcggtt taaccgcatt cacctcttac ggttctttct ggttgacgct
     1621 ggttgcgatt ctgctgatgc cgaaactggg tctgaccgat gcgccaaatg cacagttcct
     1681 tggtgtctac ctgggtctgt ggggcgtatt tacgctgttt atgttcttcg gcacgctgaa
     1741 aggcgcacgc gttctgcaat tcgttttctt tagcctgact gtgctgtttg ccctgctggc
     1801 gatcggtaac attgccggta acgccgcaat catccacttt gccggctgga ttggtctgat
     1861 ctgtggtgcc agtgcaatct atctggcgat gggtgaagta ctgaacgagc agtttggtcg
     1921 caccgttctg ccgattggtg aatcccacta attgttttga gatccccccg ctcgggggga
     1981 tttttttatt cgctaacgtc gcgtcttgca ctcttcggtc tgaatgcagc aaccacttct
     2041 ggtgccgttt caacgtatgg cccttccagc aactgaatgc agtacggtac gctggcaaaa
     2101 ataccgtgta ccacaacgtt acccgcagcg tccttcacac cttccagcgt ctctttaata
     2161 gacttcggct gaccgggtaa gttaaggatc agcgcctgtt tgcgaatcac accaacctga
     2221 cgcgaaagga tcgcagttgg tacaaaatgc aggcttatct ggcgcatctg ttcaccaaag
     2281 ccaggcatct cgcggtccgc tactgccagc gtcgcatcgg gcgttacgtc acgacgcgcc
     2341 gggccagttc cgcccgtggt gagcaccaga tggcaactca tttcatccac tagctcacac
     2401 aacgtttgct cgatgatcgc ctgctcatcg gggattaagc gggtttccag ttcaaacggc
     2461 gtggttagcg ccgatgtcag ccattcttcc agcgcaggga tgcctttatc ctgataaacg
     2521 ccgctggatg cgcgatcaga gatggaaact aagccaatac gtaaagtatt catcaagatt
     2581 tttccgttta aacagtctcg ttaaacagaa tgatacactg cgaagggagt gacagacagg
     2641 cttcagaaga ggtagcgtga ccgacttccc ggtcacgcta agaatgatta cagcagatcg
     2701 ccgatcattt tttccagttt ttcctggtca atagcaaact tacggatacc ttccgccagt
     2761 ttatctactg ccattggatc ctggttgtgc tgccacagga actcggactc agtgatacgc
     2821 gccggacgcg ctttcacttc gccggtgtaa gacagtttac gttcgatagc cccttcgctc
     2881 tcagccagct ctttcagcag tgccggtgcg atggtaagac ggtcgcagcc tgccagttcc
     2941 agaatttcgc cgatgttacg gaagcttgcg cccataacca cggtttcata accgtgctct
     3001 ttgtagtact ggtagatttc agatacagaa accacgcccg gatcttctgc cggagcgtac
     3061 tctttcttat cagtattcgc tttgtaccag tcaagaatac ggccaacaaa tggcgagatc
     3121 aggaacacgc ctgcttccgc acaagcacga gcctgagcga aggagaacag cagggtcagg
     3181 ttacagttga tgccttcttt ttccagctgt tctgcagcac ggataccctg ccaggtagaa
     3241 gccagtttga tcagaatacg atcgttgcta ataccagcat cgttgtagag tttgatcagg
     3301 cgttttgctt tcgcaattga cgcttcggtg tcataggaaa gacgcgcatc aacttcagtt
     3361 gagatacggc ccggaaccag tttcaggatt tccagaccaa tatttactgc cagtttgtcg
     3421 gtcgcgtcca cgatctgctg cgcgcgatcg ttgctctgct gtttcgccca ggcgacagca
     3481 tcatcaatca acttacggta ttccggaatc tgcgctgcgt taagaatgag agaagggttg
     3541 gttgtggcat cctgcggttg atacagcttc attgccgcga tgtccccagt gtcggccact
     3601 acggtggtgt actgacgaag ggaggtcaat ttgtccgtca tgatagtatt tctctttaaa
     3661 cagcttgtta gggggatgta accggtctgc cctgatgata tcacgacgca acgtcagcgc
     3721 aaccgccgac aagacggtta tcgccaggcg agactgtttc ggatttctga cactgcgttg
     3781 atatcgcccg ccatttttat acaaaacctc atgtatgcta cgcagaagtt atcaagtacc
     3841 tcgtagcgta tatacttctt aaacaattgg taacgtttac acaggaaagt catcgcgacc
     3901 ggcaataaga gggatatgca tgccagattt tttctccttt attaacagcg tcctttgggg
     3961 atcggtaatg atttacctgc tcttcggcgc aggttgttgg ttcacttttc gcaccggatt
     4021 tgtgcagttt cgctacatcc gccagtttgg caaaagtctt aaaaatagca ttcatccaca
     4081 gccaggcggt ttaacctcat ttcagtcatt gtgtaccagt cttgcggcgc gcgtgggtag
     4141 cggcaacctg gccggcgttg cgctggctat taccgccggt ggacctggag ccgtcttttg
     4201 gatgtgggtt gccgcgttta tcggcatggc gacctcgttt gccgaatgtt cccttgcaca
     4261 actttataaa gaacgtgacg tcaatggaca gtttcgtgga ggaccggcat ggtatatggc
     4321 gcgcgggctg gggatgcgtt ggatgggcgt tctgttcgcc gtctttttgc tcatcgccta
     4381 cggcataatt ttcagcggag ttcaggcgaa cgccgttgcc cgagccctga gttttctttt
     4441 gattttcccc cgctggtgac aggcattatt ctcgctgtct ttgctctgct ggcaatcact
     4501 cgcggtcttc atggcgtcgc ccggctcatg caggggtttg tcccgttgat ggcgataatc
     4561 tgggtgctga ccagcatggt gatttgcgta ataaatatcg ggcaacttcc ccacgtcatt
     4621 tggtctattt ttgagagtgc ttttggctgg caggaagcgg caggcggcgc ggcgggatat
     4681 accttaagcc aggcgattac taacggtttt cagcgcagta tgttttccaa tgaggcggga
     4741 atgggttcga cgccaaacgc ggcagcggca gcggcgtcct ggcctccgca tccggcagcg
     4801 caagggattg tccagatgat tggcattttt atcgacaccc tggtcatctg tacggcaagc
     4861 gccatgctga tattactggc gggtaacgac acaacctaca tgccgctgga aggtattcag
     4921 cttatccaga aggcgatgcg ggtgctaatg ggttcctggg gtgctgagtt tgttaccctc
     4981 gtggttattc tgtttgcctt cagctccatc gttgccaact acatttatgc cgaaaacaat
     5041 ctcttctttt tacgcctgaa caaccctaaa gcgatctggt gtttgcggat ctgcaccttc
     5101 gcaacggtca tcggcggcac cttgctaagt cttccgctga tgtggcaact ggcagatatc
     5161 ataatggcct gcatggctat taccaatttg accgccattt tactgctctc gcctgtggtt
     5221 cataccattg ccagtgatta tctacgccag cgtaaactcg gcgtgcgccc ggtgtttgat
     5281 ccgttgcgtt atccggatat cggtcgccag ctttctccgg acgcgtggga tgatgtttcg
     5341 caggagtaat cacaactatc gatcaactca ttctcttttt ttgctaaagt cggcataaat
     5401 ttcctgcaag gactggatat gctgattctt atttcacctg cgaaaacgct tgattaccaa
     5461 agcccgttga ccaccacgcg ctatacgctg ccggagctgt tagacaattc ccagcacttg
     5521 atccatgaag cgcggaaact gacgcctccg cagattagca cgctgatgcg catcagcgac
     5581 aaactggcgg gtatcaacgc cgctcgcttt catgactggc agccagattt cacgccggag
     5641 aatgcccgcc aggctatttt ggcgtttaaa ggtgatgtct acaccggctt gcaggccgaa
     5701 actttcagcg aagacgattt cgattttgcc caacagcatt tgcgaatgct ttccggcttg
     5761 tatggcgtac tgcgcccgct cgatttaatg cagccttatc gtctggaaat ggggatccgt
     5821 cttgagaatg cccgagggaa agatctgtat caattctggg gagatatcat caccaacaag
     5881 ctgaacgagg cgctcgcagc acaaggcgat aatgtggtga ttaatctggc gtcagatgag
     5941 tattttaaat cggtgaagcc gaagaaattg aatgccgaga ttatcaagcc ggtgttcctc
     6001 gatgagaaga acggcaaatt taagataatc agcttctacg ctaagaaagc acgcggtctg
     6061 atgagtcgtt tcattattga aaatcggctg accaaaccag agcaactgac tggttttaat
     6121 agcgaaggtt acttctttga tgaagcttcc tccagcaatg gcgaactggt gtttaaacgc
     6181 tacgagcagc gttaagtttg cagtcaatgc cggatgcggc gtgaacgcct tatccggcct
     6241 acaaaagcat gcagattcaa tatattgcag aattcacgta ggcctgataa gacgcgccag
     6301 cgtcgcatca ggcgctgaat gccggattgt tacccggcat ttgtcattta gcggtgatgt
     6361 ttgcctggac catgaccgcc gtgatgatca tgaggagctt tcttatggtg gcgcggcggt
     6421 ggcggcggtc cgtgtgggtg ccagcgattg cctcgccatt cataatgttg tttccaccag
     6481 ccgtggtcgc gccagtgacc tccatcccag taatagccac gattatcacg atcgcctatc
     6541 tgtaatttta ctgacgggac taacgtaatt tccgcagcct gtgctgccat gggagcgacc
     6601 agaaccaggg aaagtgcgag tacgatagat tgcatctttt tcacaggtta ctccttttat
     6661 cattgcccgt tatgggtcga tatccggaga ttacgcgatg agaacgacgg ttgttattct
     6721 ccgcaatcct aatctctaag ttaccgcatt tattgagaat ttctcctttt tccctgtcgt
     6781 ttttctccat aatttcttca taaaaaagcc gggctgcata aaagcaaacc cggcctgatt
     6841 gagataatga atagatttta ctgatgattc atcatcaatt tacgcaacgc agcaaaatcg
     6901 gcgggcagat tatgtgaaag caagggtaaa tcagcacgtt ctgccagctc ttttggcaga
     6961 tccaacgttt caccgagaat cgcttccacg ctctctttaa atttcgccgg atgcgcggtg
     7021 ccgaggaaca agccatattc gcctggattc aactggtcac acagcgcacg atacgctacg
     7081 gcagcgtgcg gctccgaggt gtagcccagt tcttttaact cacgcattgt ctgttgcgtg
     7141 gtttcatcat ccacggctgc ataacccagc tctttcagtt gccagatttt gcggcggaac
     7201 aactcttcca cacgcggcca gttgttcggc tgactcacgt ccatcgcgtt ggataacgtc
     7261 gcctgagtcg ctttgggtga ccactgaccg tcgtgcagga aacgtggcac ggtatcgttc
     7321 acgttggtcg cagcaataaa acgtttcacc ggcagaccga gtgacttcgc cagcagaccc
     7381 gccgtcaaat cgccgaagtt tccgcttggc accgagacaa ccagctggtt gcgcgcttcc
     7441 tgcggcagct gcgcaactgc ttcaaagtag tagcaaatct gcgccagcaa acggctgatg
     7501 ttaatcgagt tagccgagtt taaccccagc gccactttca gttcttcatc atcaaacgcc
     7561 tgcttcacca gcgcctgaca ggcatcgaaa tcgccgtcga tggcaacagt ttcgatattg
     7621 ccgcccaacg tacagaacag tttttcttgc agtggactga ttttgcctcg tggatagagg
     7681 ataaccactt tcacattcgg caaaccgtag aaagcatgag ccactgccgc tccggtatca
     7741 ccggaggtcg cggtcagaat ggtcactggc ttatcgcccg caatatgggt cagcatttgt
     7801 gccataaagc gaccgccgaa atctttaaat gccagcgttg gcccgtggaa caattccaga
     7861 caaccgacat cgctttcaac attggcgacc ggagccggga aggcaaacgc cgcgcgcacg
     7921 cgctcttcca ggatttcctg cgggatttca tcaccaataa acgccgagag gatcttcgca
     7981 ctgcgggtga caaaatccag cttcagcatc tcatcaattt cagtcaggct gaattccggc
     8041 aggtcatgcg gaaaaaacag cccctgattt ttgcccaacc cctgggttac ggcttgcgca
     8101 aagctgacct gctcgttgtg atctttcaga ttgtagagtt tcatttagtt ttccagtact
     8161 cgtgcgcccg ccgtatccag ccggcaaata tgaacaaaac cttcctgatt ttgcaggtag
     8221 ttcttaccca accagtcggc aacgcgctgg gcggtatccg gcttgtcaca tagagcgaac
     8281 aaggtcgggc cggagccgga gataccgctc gctaccgcgc cgatttccgc gaccgcctgc
     8341 cgcgcctgcc ggaagccagg cagtaaccgt tcacggtagg gttcagcgat aacatctttc
     8401 atcagcttcg cggcaagctc aggctgacgg gaatagcagg cgtgaatgaa gccagccaga
     8461 tgtcgcccgt gcgcaatgca atcctggcgg cgatactgcg ccggtaaaat agcccgggct
     8521 tctgccgtcg agactttaat tcccggatac gccagcaccc acagccactc atcaaaccct
     8581 ggcacttgct ggctgatgat gtcgttttct tcgatcatca actgcatacc accaagaaaa
     8641 cacggtgcca cgttgtcgta atgaatgctg ccggagatac gtccttccag ctcgcccatc
     8701 aaagccagca aacgggtgtc attaagtggc ttgccgcagt gttcattcat cgccatcagc
     8761 gccgcgacca ccgaacaggc gctggagcct aagcccgagc cgatcggcat attcttttcc
     8821 agagtcatcg ccactggaat ttgcttgccc agctccaggc aaaaacgctc ccagcactga
     8881 taaacgatat tttcccgtgg ttctgacggc agcttatcgg caaagcgtcc gaggttgttg
     8941 agactgaatg tctctgccgc ctcaaccgtg actacatctc cgagcaatgc accatcaacg
     9001 ggtgtcaccg ccgccccgag cacatcaaac ccgacgctca tattggcact ggaagccggg
     9061 gcataaactt taaccatgtc agactcctaa cttccatgag agggtacgta gcagatcggc
     9121 aaagacacca gcagcggtaa cgtcattgcc cgcaccatat ccgcgcagca ccaacggcag
     9181 cggctgataa tagtggctat aaaaggccag ggcgttttcg ccatttttca ctttgaacag
     9241 cggatcattc ccatccactt cggcaatctt cacgcggcag acaccatctt catcaatatt
     9301 gccaacatag cgcaaaactt ttccttcatc acgggtcttc gccacgcgcg cggcaaagag
     9361 atcgtcgagc tgtgacagat tcgccataaa agcggcaaca tcaccctcgg cgttaaactc
     9421 tgcgggcagc acaggttcaa tttcaatatc cgccagctcc agttcacgtc ccgtttcacg
     9481 agcgagaatc aatagtttac gcgccacatc cataccagaa agatcatctc gcgggtccgg
     9541 ttcggtataa cccatttccc gcgccagcgt ggtcgcctcg gagaaactca tgccttcgtc
     9601 taacttgccg aagatataag aaagcgaacc tgaaagaatg ccggagaact tcaccaattc
     9661 atcacctgca ttgagcagat tttgcaggtt ctcaataacc ggtaatccag ccccaacgtt
     9721 ggtgtcatag aggaatttac gccgcgattt ttccgccgca taacgcaact gatgatagta
     9781 atccatcgac gaggtgttgg cctttttgtt cggcgtgaca acgtggaaac cttcgcgcag
     9841 gaagtcggca tattgatccg ccactgcctg gctggaggtg cagtcaacaa tgaccgggtt
     9901 cagcagatga tattctttca cgaggcgaat taagcgcccg agattaaacg gctctttggc
     9961 ttgtgcaagt tcttcctgcc agttttccag attaaggcca tgcacactag tgagcagagc
    10021 cttcgagttg gcaacgccgc agacacgtaa gtcgatatgt ttattcttca gccagctttg
    10081 ctgacgcttc agttgctcca gcagcgcacc gccaacgcca ccgacgccaa tcacaaacac
    10141 ttcgataacc tgatcggtat tgaacagcat ctgatgagta acgcgcacgc cagtggtcgc
    10201 atcatcgtta tttaccacga cagagattga gcgttcagaa gatccctgag caatggcgac
    10261 aatgttgata ttggcgcggg ccagcgcggc aaagaatttc gccgagatcc cacgcaaggt
    10321 gcgcatacca tcacctacca ccgagataat ggccagccgt tccatcaccg ccagcggctc
    10381 cagtaagcct tctttcagtt ccaggtagaa ctcttcctgc attgcccgtt cagcccgcac
    10441 acagtcgctt tgcggaacgc agaaactgat actgtattcg gaagatgatt gcgtaatcag
    10501 caccacggaa atacgggcgc gtgacatcgc ggcaaagacg cgcgccgcca tgccgaccat
    10561 tcctttcatc cccgggccgg aaacgctgaa cattgccatg ttattcagat tggaaatgcc
    10621 cttgaccggt aattcgcctt catcacgact ggcaccaatg agcgtacctg gtgcttgagg
    10681 atttccggta tttttaatca ggcaagggat ctggaactgg gcgatggggg taatggtgcg
    10741 ggggtgaaga actttagcgc cgaagtagga aagctccatc gcttcctggt aggacatcga
    10801 cttcaacaac ctcgcatcgg gcacctgacg cgggtcgcag gtatagaccc cgtcaacgtc
    10861 cgtccaaatc tcgcaacaat cggcgcgtaa acaggcagcc agcaccgccg cggagtagtc
    10921 ggaaccgttg cgtccaagta ccaccagttc gcctttttca ttaccggcgg tgaagcctgc
    10981 catcagcacc atgtgatcag ccggaatacg acttgccgca atacggcggg tggactcagc
    11041 aatatcgacg gtagattcga ggtaatgccc cactgccagt agtttttcga ccggatcgat
    11101 aacggtaacg ttgtgaccac gcgcttctaa cacgccggcc ataatggcga tcgacatttt
    11161 ctcgccacgg caaatcagcg cagcgttaat gctatccggg cactgcccta acaaactaat
    11221 gccatgcagg acatgtttta tttgggcaaa ttcctggtcg acgaaagttt tcaattgcgc
    11281 cagcgggaat cccggctggg cggcggcgag tcccgtcaaa agttcggcaa aaatacgttc
    11341 ggcatcgctg atattgggta aagcatcctg gccgctaatg gttttttcaa tcatcgccac
    11401 caggtggttg gtgattttgg cgggggcaga gaggacggtg gccacctgcc cctgcctggc
    11461 attgctttcc agaatatcgg caacccgcag aaaacgttct gcatttgcca ctgatgtacc
    11521 gccgaacttc aacactcgca tggttgttac ctcgttacct ttggtcgaaa aaaaaagccc
    11581 gcactgtcag gtgcgggctt ttttctgtgt ttcctgtacg cgtcagcccg caccgttacc
    11641 tgtggtaatg gtgatggtgg tggtaatggt ggtgctaatg cgtttcatgg atgttgtgta
    11701 ctctgtaatt tttatctgtc tgtgcgctat gcctatattg gttaaagtat ttagtgacct
    11761 aagtcaataa aattttaatt tactcatggc aggtaaccag ttcagaagct gctatcagac
    11821 actctttttt taatccacac agagacatat tgcccgttgc agtcagaatg aaaagctgaa
    11881 aaatacttac taaggcgttt tttatttggt gatatttttt tcaatatcat gcagcaaacg
    11941 gtgcaacatt gccgtgtctc gttgctctaa aagccccagg cgttgctgta accagtcgac
    12001 cagttttatg tcatctgcca ctgccagagt cgtcagcaat gccatgactc gttcgcgtaa
    12061 agcttgcagt tgatgttggt ctgccgttgt gtcacttttc gccggttgtt gtattaatgt
    12121 tgctaattga tagcaataga ccatcaccgc ctgccccaga ttgagcgaag gataatccgc
    12181 caccatcggc acaccagtaa gaacgtcagc caacgctaac tcttcgttag tcagcccgga
    12241 atcttcgcga ccaaacacca gcgcggcatg gctcatccat gaagattttt cctctaacag
    12301 cggcaccagt tctactggcg tggcgtagta atgatatttc gcccgactgc gcgcagtggt
    12361 ggcgacagtg aaatcgacat cgtgtaacga ttcagccagt gttgggaaaa ctttaatatt
    12421 atcaataaca tcaccagatc catgtgcgac ccagcgggtg gctggctcca ggtgtgcctg
    12481 actatcgaca atccgcagtt cgctaaaacc catcgttttc attgcccgcg ccgctgcccc
    12541 aatattttct gctctggcgg gtgcgaccag gataatcgtt atacgcatat tgccactctt
    12601 cttgaccaaa taaccgcgaa cagggtgatc actgtcaact tattacgcgg tgcgaattta
    12661 caaattctta acgtaagtcg cagaaaaagc cctttactta gcttaaaaaa ggctaaacta
    12721 tttcctgact gtactaacgg tttaattgtt aaaaaatgct acatatcctt ctgtttactt
    12781 aggataattt tataaaaaat aaatctcgac a
//
LOCUS       DAZRUF010000118        12617 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZRUF010000118 DAZRUF010000000
VERSION     DAZRUF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.118"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..330)
                     /locus_tag="ACHXOL_002596"
     CDS             complement(<1..330)
                     /locus_tag="ACHXOL_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFR6809550.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIYMNASRLQGIHCFDMTGRLQLYIRLYCSRNAA
                     SPYVIRRL"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHXOL_002597"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHXOL_002597"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR6809551.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHXOL_002598"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHXOL_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR6809552.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHXOL_002599"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHXOL_002599"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR6809553.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHXOL_002600"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHXOL_002600"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR6809554.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHXOL_002601"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHXOL_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR6809555.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHXOL_002602"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHXOL_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR6809556.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHXOL_002603"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHXOL_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR6809557.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHXOL_002604"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHXOL_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR6809558.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHXOL_002605"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHXOL_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR6809559.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHXOL_002606"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHXOL_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR6809560.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHXOL_002607"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHXOL_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR6809561.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHXOL_002608"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHXOL_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR6809562.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2906 a         3508 c         3076 g         3127 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag cacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat ggcacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaactt
    12361 tatttttatg cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg taaaaaataa
    12421 ttagcattag aatagttgcg ataagctgca ataagcagaa ccaccttttt ggtttaatat
    12481 gtccttacaa atagaaatgg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
    12541 ctgtactcgt cactacacgc acatacaacg gagggggggc tgcgattttc aataatgtgt
    12601 gatgcagatc acacaaa
//
LOCUS       DAZRUF010000119        12250 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZRUF010000119 DAZRUF010000000
VERSION     DAZRUF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.119"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..160
                     /locus_tag="ACHXOL_002609"
     CDS             <1..160
                     /locus_tag="ACHXOL_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HFR6809563.1"
                     /translation="GFITPDDGSKDVFVHFTAIQSNEFRTLNENQKVEFSIEQGQRGP
                     AAANVVTL"
     gene            171..359
                     /locus_tag="ACHXOL_002610"
     CDS             171..359
                     /locus_tag="ACHXOL_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR6809564.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            334..564
                     /gene="ymcE"
                     /locus_tag="ACHXOL_002611"
     CDS             334..564
                     /gene="ymcE"
                     /locus_tag="ACHXOL_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR6809565.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            554..727
                     /locus_tag="ACHXOL_002612"
     CDS             554..727
                     /locus_tag="ACHXOL_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR6809566.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(776..1849)
                     /locus_tag="ACHXOL_002613"
                     /pseudo
     CDS             complement(776..1849)
                     /locus_tag="ACHXOL_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1921..4665)
                     /gene="torS"
                     /locus_tag="ACHXOL_002614"
     CDS             complement(1921..4665)
                     /gene="torS"
                     /locus_tag="ACHXOL_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR6809567.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFATQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            4748..5776
                     /gene="torT"
                     /locus_tag="ACHXOL_002615"
     CDS             4748..5776
                     /gene="torT"
                     /locus_tag="ACHXOL_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR6809568.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5749..6441)
                     /gene="torR"
                     /locus_tag="ACHXOL_002616"
     CDS             complement(5749..6441)
                     /gene="torR"
                     /locus_tag="ACHXOL_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR6809569.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6561..7733
                     /gene="torC"
                     /locus_tag="ACHXOL_002617"
     CDS             6561..7733
                     /gene="torC"
                     /locus_tag="ACHXOL_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR6809570.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNESDTAGKAHGDKKDEK"
     gene            7733..10144
                     /gene="torA"
                     /locus_tag="ACHXOL_002618"
                     /pseudo
     CDS             7733..10144
                     /gene="torA"
                     /locus_tag="ACHXOL_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10145..10690
                     /gene="torD"
                     /locus_tag="ACHXOL_002619"
     CDS             10145..10690
                     /gene="torD"
                     /locus_tag="ACHXOL_002619"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR6809571.1"
                     /translation="MFSRELDDEQLTQISSAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(10783..11088)
                     /gene="cbpM"
                     /locus_tag="ACHXOL_002620"
     CDS             complement(10783..11088)
                     /gene="cbpM"
                     /locus_tag="ACHXOL_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR6809572.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11088..12008)
                     /gene="cbpA"
                     /locus_tag="ACHXOL_002621"
     CDS             complement(11088..12008)
                     /gene="cbpA"
                     /locus_tag="ACHXOL_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR6809573.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2932 a         3161 c         3224 g         2933 t
ORIGIN      
        1 tggctttatc actcctgatg atggcagcaa agacgttttc gtccatttca ccgccatcca
       61 gagcaatgaa ttccgcacgc tgaacgaaaa tcagaaagtt gaattttcta ttgagcaggg
      121 gcaacgtggc cccgcggcag cgaacgttgt tacgctctaa ggttgccgtt attactcaac
      181 atctccattt ccgctgtcca tgttgtcatg gttcacagta ccgcacatcg gcattcgatg
      241 tgacggagcg aaaccctttg ggcgctaagt gtattttttg taaatcgacg atgatcacct
      301 ttgataacgt cgcgctgcaa atacgcactg accatgcgcc gctggatttc acaaaataat
      361 atcaggctcc ctcgtggagc cttttttata tctgccttat ttttcttcaa cgctgtatgt
      421 atagtaagcg ataacctgtt gattattgaa tctttcgggg agatggctta taacatttct
      481 tacctgacca gggtaccggg aaccaacacc ttactggcgt gttgctgtct tttaagacca
      541 gaagaggtta acagtgaata ttgaagagtt aaaaaaacaa gccgaaacgg aaatcgccga
      601 ctttatcgcg caaaaaatcg ccgagctgaa caagaataca gggaaagaag tctctgaaat
      661 tcgcttcacc gcacgagaaa aaatgaccgg gcttgaaagt tatgatgtca aaatcaaaat
      721 aatgtgattt tgtgaacatc accccgtgcg aggtgatgtt ccgcttgttg ctaatttagt
      781 gaccaatcat tggcgcttgt ggaattaagc gtcggtacaa ttcctccggc accgggcttt
      841 gccatactcc cgcatacatt gcgtaaccaa tcaccgcgaa cataatcctc agaaccagta
      901 gcgtcattaa ccagccagac aacgcaaaag cttttttatt tgccgcaggt ttttgcagtg
      961 aaaaggtcaa tgttgaggct accggacatg actctacgca agtcatacag ccggtacatt
     1021 ccactgttcg tacctgaatt aatttatcta ccgggatccg tgatgggcaa ttttttgcgc
     1081 atttgccaca gccgatacaa ctttcggcat tgcgacgaat cttaaacggc gataatagcg
     1141 aaaccacgcc catcagcgcg ccatatgggc aaagataacg acactaggca tggcgaataa
     1201 acaggctggc aatcagcaaa acggtcacgc tgattaatgt cgcggtcccc atatgacgaa
     1261 agaaatcgag catttttacg tccatcacca cgctgtaggg cgacaacata aaatagtgaa
     1321 tcgcctgagc gggcatcaat aacgcgatat agagaaaaaa actcaacagc aaatacttca
     1381 cgccgcgcag aggaatatcc agccagcggg gaaggacaca ttgccgacca aacagtttgt
     1441 taccgagatc gccgattaat tcagaaagcg taccaaccgg gcataaccat gagcaaaagg
     1501 cctttttgag taatagactg atgacgataa aagcgaccaa taacagcatc gcggcggcgt
     1561 ggacggacgg taactgacct gttgtaaggc tatatttcag attcatcaga ccggcaatcg
     1621 gtagccagcc ttcgatacct cccggtctgg cgacaaatgt cgtgctactt gccgtttcgt
     1681 aatagcgcac ccaataccag aacgtgatgg caatataaat attcattgcc aacagtaata
     1741 attgcgtcgc tttacgccag gtcgtggcat tgcgccagtc attccacggt aatttgccgc
     1801 ccgtcgtgcc tggccgccgc tgccagcggg ttcttttttt ctctgccatg attttgccag
     1861 tccgttaagt tgcataccaa atgccactat tctagttgtt cttaactggc tgatattgat
     1921 tcaaatcgcg ttcaggtctt tcttatgcaa ccatgcttcc agagcggcaa cactgcgtgt
     1981 aatttcttcg tgtggaaggg gggcagataa tggctgctgc tccagttgtg cgcatagctg
     2041 gctggcgata cgcattccca aactcgagca actgcttttt agctgatgcg cggcacgctt
     2101 tattttctcg ctatcctgac tggcgcgggc aatgtcgatt tcatcgagaa gcggcagggc
     2161 atgttgtgta aataatacca gccattcgtg gatcttctcc gtccccatta actgagcatc
     2221 ttcattgagt tgcgatacat ccagcgattg atcgttattg acttgcagtt ggagatagtg
     2281 cgccagtaac tgaccgagca cttcacgcgg caccggttta gggataatcc cgcggaataa
     2341 tgaactggta cgctggcgca gcgtttcgtc aatgacatgg gcgctaaagc caatcaaaac
     2401 cagcgacgga tattgctgtg ccagttgtcg ggcaagcgta atgccgtcga catccggcag
     2461 atcaaaatcc accagtgcgg cagcaaacgg ttcgctattt tgcagtgtct ctaaagcctg
     2521 cgcggcattg ccaacagcaa caacctgcgc accactggtg ttcagcatct caacggtaat
     2581 tcgctgggtt agcgggttat cttcaattaa cagcaaacgt aaaccgtcaa gacgcaccgc
     2641 ctgattgact gtttttggca cgggtgccgt ggcaacacgt aacggcaagc gtaaacaaaa
     2701 acagcttcca acctccggcg tgctggtggc gctcagttcg ccgcccatcg cctgggccag
     2761 acggctactg atagtcagcc ccagcccggt gccgccgcgt ttgccgctta cctgcacaaa
     2821 tggctggaag atttctgcca gtttcgcggg atcaataccg cagccgctgt cttccacttc
     2881 gaccagccat tgctcgccat cagtgcgact acgcaggata atgtaccctt cgtcagtaaa
     2941 acgcagggcg ttgctcaaca ggttggttat aacctgacga atacgtcgtg gatcgcccat
     3001 taacgcgcac ggcatatcat cggcaattgc cgttgccagg cgaatcgggc gacctttcac
     3061 ccgtccgctc attaattgca gggtactttc cagcagcggg cgcggttcaa agggctcatc
     3121 gctgaccgaa acattcttgc cacctgcttc gatagcggaa taatcgagaa tatcgttgag
     3181 gatggtcagc aacgattcac cagagtcagt aattgcccgc aaatcatcac gctgggcgtt
     3241 aagtgcgggg ttatctgcca gtagctgtgc ggtgccgaga ataccgtaca gcggtgtgcg
     3301 gatctcatgg ctcatcgccg ccagaaacgc cgattttgcc tggctggctt tttctgcttc
     3361 cgcccgtgcc tgtcggtgtt ctatcactag ttcctgcaat tcagctgtac gcgctttgac
     3421 ctgcgccgcc agctgttcac ggtggcgatt cagtgcatga acactgctgc gaaacgcacc
     3481 catcagctgc ccgatggtgt ccagctcccg tacgccagcg gtttccggga aaggggagtc
     3541 aatatcaccg tccagcagcc gttgcagcgc ctgcgtttgt tcggcaagtg gacgcgtgac
     3601 tgagcgataa accacgcgcc agaggatcag aatcagtgcg caaagtgaaa ccatccccag
     3661 caataacagg ctgtattgcc cgcgtgcact ggctttttcc tgatgcgcca gtccgtgctg
     3721 attacgcagc tcaatggtgt cgaccagctg actgacttcg ctactaaact gcgcgaactg
     3781 ggcgatatta ttttgggcga gagtttgcag gtgattgctg atttcactgt cctgctggaa
     3841 cagcgccaac agatcgctat attggctaac ggtagttaac gttgttgcga cctgcgcacg
     3901 aactcccgga tcttcaatgc gtatttgccg acgttgcaga attttcaccg cattattgag
     3961 ctgcttttcc agcgttggtg catttttctg gatctgctcc agccccagat tcatcaccat
     4021 ttgctgcacc cgcagagcgc taaggcgcag ttcattcatc tggttaacat actcaagatc
     4081 gatatcaatc agccgatcga gtgcactttc agcagcctga cgctgatctt gttcgatcaa
     4141 atcgtaaatc ccggcctggg tcgctccagc ggaagttgtc gcattattcg cctgaccttg
     4201 cgccaggcgt gcgatctcat cggcggcagc gactatctgc tgactgagtt gctgttgttg
     4261 ctggcgtagt tgcagacgcc gccccaccag ttccccttgc tgacgtaacg aacgggagat
     4321 ctcctgctcc tgttgttcaa tagcggtggt atcaaaacct tgttcccgta acgcttgcag
     4381 caacgcatta atcttcaggc tttgtgcggt gagcattcgc ccctgcgcct gccacatctt
     4441 ttcgttatcg gcactggtca ggttctgcgt ggcgaaaagt tcccaggcgc tggcttcgct
     4501 caactggcgc gccatattca tggtaggaat caatgcctga gtattgtctt tttccacctg
     4561 gctgataaag cgcaggttgt accatcccac cagggtactg gtcagggtta acagcgccat
     4621 cagggcaaag cccatccaga gtcttcgggt cagggttaaa ttcacggtcg gtgcacttta
     4681 ggtgaaaaat gttgagtcgc aaagcggaat gcatctagca taaagcctta ttattgatga
     4741 ggctatcatg cgcgtactgc tatttttact tctttccctt ttcatgttgc cggcattttc
     4801 ggctgataac ctgttgcgct ggcatgatgc gcagcatttc acggtgcaag cctctatgcc
     4861 gcttaaagcc aaacgcgcat ggaaactgtg cgcgctttat cccagcctga aagattcata
     4921 ctggttatcg ttgaactatg gtatgcagga ggcagctcgc cgttatggtg tggatttaaa
     4981 agtgctggag gcaggcggct acagccagtt ggctacccag caagcacaaa tcgaccagtg
     5041 taaacagtgg ggcgcagagg ccattttact cggtagtagc acgacctcat ttcccgatct
     5101 gcaaaagcag gtagcaagtc tgccggtgat cgaactggta aatgctattg atgctcccca
     5161 ggtgaaaagc cgcgttggtg taccctggtt tcagatgggc tatcaacctg ggcgatattt
     5221 ggtgcaatgg gcgcacggta aaccactgaa cgtgctgttg atgcccggac ccgataatgc
     5281 cgggggcagt aaagagatgg tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg
     5341 tattgttgat attgcgcttg gtgataacga tattgaaatc cagcgtaacc tgttgcagga
     5401 gatgctggaa cgccacccag aaatcgacgt cgttgccgga acggccattg cggcagaggc
     5461 ggcaatgggt gaagggcgta acctgaaaac gccgcttacc gtggtgtcgt tttatctttc
     5521 acatcaggtg tatcgcgggc tgaagcgggg aagagtgatt atggctgcca gcgatcaaat
     5581 ggtctggcag ggggaactgg cggttgagca ggccatcagg caattacagg ggcaatcggt
     5641 gtctgataat gtcagcccac cgattttagt tctgacgccg aaaaatgccg accgtgaaca
     5701 tattcgccgc tcgctgtcac cagggggatt tcgtccggtc tatttttatc agcacacatc
     5761 agcggctaag aaataacctt caccatgttg cgtcaccagt aaatccgcgc tgagtttatg
     5821 acgtaaacga cgaattaaca catcgacggt gcgcaggtca gggttttcca cccgacgcgc
     5881 agaaagcata cgtagcagac gttcacggct gagaatttcg cccggattcg tcacaaatgc
     5941 caccaacatt tcatactctg cgcgggtcag tttaatcggc tcgccatccc gctccagcgt
     6001 atggcgcgac acattcaggc aataaccggc aaagcgatag cagttgtcct gagtgtgcgg
     6061 ttgagcttgt cgcgcgaggt cgattcgcca gagcagattt ttcacccgta ctaccagttc
     6121 gcgcagttcc agcggtttgg tgacgtaatc gtctgcgccc atttccagcc caacaatacg
     6181 gtcaatccga tcgctgcgtc cggtaaccag aataatcccc accgttgagc gttctcgcag
     6241 ggcgcgggtt aacatcaggc cattttcatc gggtaagttg atatccagca gaattaaatc
     6301 taccggctga ttctgcataa tttcccgcag cccggcaccg ctcgctgtaa cggaaacggt
     6361 atacccctcc tgagtgaagt aagattgtaa tcgcgcctgg gtaaccggct catcttcaac
     6421 aataacaatg tgatgtggca tcagagggtt ttactcattc tgttcatatt ctgccgtaag
     6481 ccgttcatcc tgaccagtgc cgctgttcat atttgctcat taagatcgct tcactaaacc
     6541 ataattttac aggggctatt atgcggaaac tctggaacgc gctacgccga cccagtgctc
     6601 gttggtcggt actggcgctg gtcgctattg ggattgtgat tggcattgcg ctgattgtat
     6661 tgccacacgt tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt tgccacagta
     6721 tgcaaccggt gtatgaagaa tataaacagt cggtgcattt tcagaacgcc tctggcgtgc
     6781 gagctgaatg ccatgactgt catatcccgc cggatattcc aggcatggtg aagcgcaaac
     6841 tggaagcgag caacgacatt taccagacct ttattgccca ctccattgat acacctgaaa
     6901 aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca
     6961 actcggcaac ctgtcgctcc tgccataact acgatgcgat ggatcatgcc aggcagcatc
     7021 ctgaagcggc acgtcagatg aaggtggcag cgaaagataa tcaatcctgc atcgactgtc
     7081 ataaaggtat tgcccaccag ttaccggata tgagtagcgg cttccgtaag cagttcgatg
     7141 agctgcgcgc cagtgctaat gacagtggtg acacgctgta ctctattgat attaagccaa
     7201 tttatgcggc gaaaggcgat aaagaagcct ctggttctct gctgcctgct tcggaagtga
     7261 aagtccttaa acgtgacggc gactggctgc aaattgaaat taccggctgg acggaaagcg
     7321 ccggacgtca gcgtgtactc acccaattcc caggtaaacg catctttgtt gcctcgattc
     7381 gtggtgatgt gcagcagcag gtgaaaacgc tggagaaaac caccgttgcc gacaccaata
     7441 ccgagtggag caagttgcag gccaccgcgt ggatgaagaa aggcgacatg gtgaacgata
     7501 tcaaaccgat ctgggcctat gcggattcgt tgtacaacgg cacctgtaac cagtgccacg
     7561 gcgcaccgga aatcgcccac tttgacgcta acggttggat cggcacgctc aacggcatga
     7621 ttggctttac cagcctcgat aaacgtgaag aacgcacctt gttgaaatat ctgcaaatga
     7681 atgagtctga caccgcaggt aaggctcacg gcgataagaa ggacgaaaaa taatgaacaa
     7741 taacgatctc tttcaggcat cacgtcggcg ttttctggca caactcggcg gcttaaccgt
     7801 cgccgggatg ctggggccgt cattgttaac gccgcgccgt gcgactgcgg cgcaagcggc
     7861 gactgaggct gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg
     7921 cgcgacggtg aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc
     7981 gtcgaaaatg attgccggat tgccggatca cgtacacaac gcggcgcgta ttcgttatcc
     8041 gatggtacgc gtggactggc tgcgtaagcg ccatctcagc gatacctccc agcgcggtga
     8101 taaccgtttt gtgcgcgtga gctgggatga agccctcgac atgttctatg aagaactgga
     8161 acgcgtacag aaaactcacg ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac
     8221 ggggatgttc cataacgctt cggggatgct ggcgaaagct attgccttgc atggtaatag
     8281 cgttggtacg ggcggagatt actctaccgg tgctgcgcag gtgatcctgc cgcgcgtggt
     8341 tggctcaatg gaagtgtatg aacagcaaac ctcctggccg ctggtattgc agaacagcaa
     8401 aaccattgtg ctgtggggct ctgatttgct gaaaaaccag caagcgaact ggtggtgccc
     8461 ggatcacgat gtttatgaat attacgcgca gctaaaagcg aaagtcgccg ccggtgaaat
     8521 tgaggtcatc agcatcgatc cggttgtcac atccacccat gagtatctgg ggcgcgagca
     8581 tgtgaagcac attgcggtta acccgcaaac tgatgtgccg ctgcaactgg cgctggcgta
     8641 tacgctgtac agtgaaaacc tgtacgacaa aaacttcctc gctaactact gtgtgggttt
     8701 tgagcagttc ctgccgtacc tgctgggtga gaaagacggt cagccgaaag atgccgcata
     8761 ggctgaaaaa ctgaccggca ttgatgccga aaccattcgt gggctggcgc ggcagatggc
     8821 ggcgaacaga acgcaaatta ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca
     8881 gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg caaattggcc tgccaggtgg
     8941 tggctttggt tttggctggc actataacgg cgcaggcacg ccggggcgta aaggcgttat
     9001 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgattacaa
     9061 aggctacagc agcaccattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
     9121 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
     9181 tggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
     9241 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
     9301 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
     9361 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg cagttcgagg cgcgcaacga
     9421 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgc gaagaagcct ttaccgaagg
     9481 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
     9541 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
     9601 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
     9661 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
     9721 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
     9781 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
     9841 gcagttatgt gagtcggaaa ctctgcgtca gcaatatacg gtagcgggta aagagccagt
     9901 attcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
     9961 ctttaacgct cgcggtcagg tgttggcagg ggcagtggtt tctgaccgct atgcacccgg
    10021 cgtggcacga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    10081 gctgtgcaaa tacggtaacc ccaacgtgtt gaccatcgac atcggtacat cgcagctggc
    10141 gcagttgttc tcccgtgaac tggacgatga acaactgacg caaatctcca gtgcgcagat
    10201 ggctgaatgg ttttcgttgc tgaaaagcga accgccgctc actgcggcgg tgaatgcgct
    10261 ggaaaaccgt attgccgctc tgacagtacg tgacgatgcc cgtctggaac tggccgctga
    10321 cttttgcggc ctgtttctga tgaccgacaa acaagcggcg ctgccgtatg catcggccta
    10381 caaacaggac gagcaagaga ttaaacgctt gttagttgag gcagggatgg aaaccagtgg
    10441 caatttcaac gaatcggcag atcatctggc gatctatctc gagttgttga gtcatctgca
    10501 tttttcgctg ggagagggga ccgttcctgc gcgaagaatc gacagtttgc ggcaaaaaac
    10561 actgacggcg ctgcggcaat ggttaccaga gtttgctgcg cgttgtcgac agtatgacag
    10621 ctttggtttt tacgcggcac taagccagtt attgctggtg ttagtggagt gcgaccacca
    10681 aaacagataa cgtcgtttat gcgcctgaaa agacgcgttt agcgtcgcat caggcattat
    10741 ggcgcagttg ccggatgcgt catcacatcc ggcaaacaca catcacggat gagctacaaa
    10801 ccgggaaagc cgctggcgta gcaggcggtt ttcctgcttc aggtgcgcaa tatcatccat
    10861 taacgtcagc gccaccgcga tccccggcca gtccagagcc agttcatgac gcaggcgtac
    10921 cgcgcgttgc accacaatgg cggcatggtc gtcaaatacc caggttgttt cctggatctc
    10981 acgcggttca accaccccca aaccgacaat ttcattcaac tcctcttcag agatgccggt
    11041 atgcaggcaa aattcggtaa tagtaaaagt caccgtaaca ttagccatca tgctttcccc
    11101 caatctttac gtggatcaaa agacgactgg gcgtctgcca gttgctgcca cagcgcggca
    11161 gtgttttcat ccggtttcgg cggcatcacg attttcagta ccgcatacag atcgccggtc
    11221 tgttttttgc tcaccagacc tttgccttta acgcgcaatc gttgcccggc ctggctgcct
    11281 ggcgggatag tcagcaaaat gctttctttc agtgttggaa cggtgacttt agcacccagc
    11341 gccgcttccc acgggctaac cggcaccaca atttccagat cctggccgac aatatcgaac
    11401 agcggatgtg gcgcaatatg aatcaccagc cacaaatcgc catttggacc accgttttcg
    11461 cccggcgtcc cctggccttt cagacggatg cgttgaccat tgccgacgcc cgccgggatc
    11521 ttcacattca gcgttttcgg gatttcctgt tcgatcatgc caaaggcgtt ataaaccggc
    11581 aggttatagc tgatggtacg cttatgctca gtcagcgttt cttcgaggaa taccgccact
    11641 tcgatttcaa tatcgtggcc gcgtgcggcg gggcgttgac ggctctggcg ggcatgctga
    11701 ccgaaaattg acgagaagat atcgtcaaaa tcttcggcat taaaactctg accgtcgcca
    11761 tgatggaact gatggttaaa ttgcggatcg ttgcgatgtt gccacatctg atcatactca
    11821 gcgcgacgtt gttcatcact taacacttcc caggcttcag cgacctcttt gaagcgggct
    11881 tcggcatccg gttctttgct gacatcagga tggtatttac gggccagtcg acgataggcg
    11941 gtcttgattg tcttgagatc gtccgtcggt ttcacgccca tgatggcgta ataatccttt
    12001 aattccatag cgttatctcg cgtaaatcaa cacaaattga aggaacccct gtaaggtaac
    12061 tcctataagt gtagggtaat cctcaaaatt tcatatgcca acacagaata tgttattgaa
    12121 atcatcgcgg agaggaggtc gccatcaaga tgggttgctg aacatatttt aaacaggtga
    12181 aaaagggtga gcgatttttg atagttgaac caggcacttt aagtttaact agggcgtcat
    12241 tatttattaa
//
LOCUS       DAZRUF010000120        12192 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZRUF010000120 DAZRUF010000000
VERSION     DAZRUF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.120"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..72
                     /locus_tag="ACHXOL_002622"
     CDS             <1..72
                     /locus_tag="ACHXOL_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFR6809574.1"
                     /translation="LYEALRTVDRLLRGQSFIKQADL"
     gene            83..370
                     /locus_tag="ACHXOL_002623"
     CDS             83..370
                     /locus_tag="ACHXOL_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809575.1"
                     /translation="MTKDNAQGVFIRFIDFRGELLLRASAIDGVTPAGKNGADEATYV
                     YLNGTRLLVELPYQTVREIISEDEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            382..624
                     /locus_tag="ACHXOL_002624"
     CDS             382..624
                     /locus_tag="ACHXOL_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFR6809576.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            621..734
                     /locus_tag="ACHXOL_002625"
     CDS             621..734
                     /locus_tag="ACHXOL_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809577.1"
                     /translation="MSIFISWLVLIISVVCAIGIMRIINSVKKIERFFTEE"
     gene            821..1024
                     /locus_tag="ACHXOL_002626"
     CDS             821..1024
                     /locus_tag="ACHXOL_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809578.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVVQVNLIRQVQESRR"
     gene            1021..1266
                     /locus_tag="ACHXOL_002627"
     CDS             1021..1266
                     /locus_tag="ACHXOL_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR6809579.1"
                     /translation="MTILDYIAANPGCSSGEIAAALNTPTTTINAELRRLWRSGLVTR
                     KERKTGGRFSYQVNLMPFGCSNPLTQMFNQLLREIRA"
     gene            1292..1411
                     /locus_tag="ACHXOL_002628"
                     /pseudo
     CDS             1292..1411
                     /locus_tag="ACHXOL_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1408..1773
                     /locus_tag="ACHXOL_002629"
     CDS             1408..1773
                     /locus_tag="ACHXOL_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809580.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            1780..4602
                     /locus_tag="ACHXOL_002630"
                     /pseudo
     CDS             1780..4602
                     /locus_tag="ACHXOL_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
     gene            4679..5638
                     /locus_tag="ACHXOL_002631"
     CDS             4679..5638
                     /locus_tag="ACHXOL_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR6809581.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGMQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            5643..5954
                     /locus_tag="ACHXOL_002632"
     CDS             5643..5954
                     /locus_tag="ACHXOL_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR6809582.1"
                     /translation="MTTPTRRISFYLKPAAVKNESEACAWLGSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTERPVPQPVPTAQITSQIAGNIRALFP
                     E"
     gene            6015..6539
                     /locus_tag="ACHXOL_002633"
     CDS             6015..6539
                     /locus_tag="ACHXOL_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809583.1"
                     /translation="MKKYEYMLMKEALRAGLAPCPVSVEAVVGNTIFRTLRNRVSVKM
                     VHQPPKSTSMIYLPLPEAIKLMRSLKFAIWMQTQINKAVQNTFVKRLIPELAARCAKC
                     TRPALQFDLSLIQQATEAELYRLSPIRDAHTEASEMFRLMFQFNFAVRRTRVQVNRMM
                     QEQEELTQYKGGSQ"
     gene            6536..6775
                     /locus_tag="ACHXOL_002634"
     CDS             6536..6775
                     /locus_tag="ACHXOL_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809584.1"
                     /translation="MSNSARLQLGFSPLSKTIVLAKMRDLGDGTKRRVGNDRGRDVTN
                     EAAQLVWHLVMAEGGEINWELDDGSRMILRAEKSK"
     gene            complement(7467..8513)
                     /locus_tag="ACHXOL_002635"
     CDS             complement(7467..8513)
                     /locus_tag="ACHXOL_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR6809585.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(8513..10264)
                     /locus_tag="ACHXOL_002636"
     CDS             complement(8513..10264)
                     /locus_tag="ACHXOL_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR6809586.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATATGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAEMHFLGTSAATAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTCFS
                     TPSTETHEAYAYWNGDRWNEKKAAHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFRNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDATGGHGDAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            10419..11255
                     /locus_tag="ACHXOL_002637"
     CDS             10419..11255
                     /locus_tag="ACHXOL_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR6809587.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVESVATS
                     QADIIDRFNALETRHQQDSQKITLLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            11279..>12192
                     /locus_tag="ACHXOL_002638"
     CDS             11279..>12192
                     /locus_tag="ACHXOL_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFR6809588.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKDSTELTKKINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWSAHSDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTNASERVMNDVTLTSRNMDNTVAHAGKYANPDALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQ"
BASE COUNT         3100 a         3300 c         3198 g         2594 t
ORIGIN      
        1 ttatacgaag cacttcgcac ggttgatcgt ctgcttcgcg ggcaaagttt tattaaacag
       61 gctgatttat aacaggtgct ttatgaccaa agacaatgca caaggtgtat ttatccgttt
      121 tattgatttt cgcggtgaac tgttattacg tgcatccgct attgacggag tgactccggc
      181 gggtaaaaac ggagccgacg aagccactta cgtttatctg aacggcacgc gactgcttgt
      241 ggaacttccg taccagaccg tacgagaaat catcagcgaa gatgaaaagg cacgccaggt
      301 taatggcgat gaaccctata tcgaaattat ttgtatggat tcagaagctg aaatacagaa
      361 agcagattaa agggcgttgt gatgggcaaa gaatataaaa ctctcattaa caaagcactt
      421 gagcgttttt attttcgctt aagtgcatca ggcgctcatg ctgaacgtgc ggcccgtgac
      481 tcattgacca gagcaatccg aagtctgtat gacgtggctt tttacgctga tgatctggat
      541 gcacttaacg aactttccga gctgatctgt gccgcagaat gcggggaaca tattgaaccg
      601 tataagctgg gaaatatcgc atgagtatat ttatttcatg gcttgttctg attatttcgg
      661 tggtctgcgc cattgggatt atgcgaatta ttaattcagt aaaaaagatt gaacgctttt
      721 tcactgaaga ataaccgcgc aaataagacc ccaggttaaa taagaaaatg tgaaaacaat
      781 ccgcattcgc ggaggtattc gcacacgcca aggaggcgta atggcaatta agcattttcc
      841 tgtcgttcgt ttcacctcca gaggacgtga atacgaagtt gacgaacgcc tgattaccac
      901 aatcgacaaa caccgttcag aaaaggatgc acatcatatc tatctcactg acggcactta
      961 cttctgcgcc accaacgtgg tgcaggtgaa tcttatcaga caggtacagg agtcacgcag
     1021 atgaccattc tggactacat cgctgccaat ccgggttgta gcagtggaga aatcgccgca
     1081 gcactgaata caccaaccac aaccattaat gcggagctac gccgtctctg gcgcagcggt
     1141 ttagtcacaa gaaaagagcg caaaacaggc ggtcgctttt cttatcaggt aaacctgatg
     1201 ccgtttgggt gtagcaaccc actaacccag atgttcaacc agctactgag ggaaatcaga
     1261 gcatgagcac ctccaactgc cggaaaccac gtcgggcttc agcagctcat ccggcagcaa
     1321 aacaaactcc attaattcct gttccgggcc tttcctgcac cttgcggcgg gaggccttcg
     1381 cacatctgta acaagaggat tgccgcaatg attctcgcca acgactttct tgaatacctg
     1441 ctcaacacag agcgtgatct tgccgctcgc gtgcgtgatc gttatgacat gtacctgaaa
     1501 tccctgcctg taccgcagct cgctgacgga aagattgtta ttgatggtcg ctacatgatt
     1561 gacagccacg agggaaatta caggctttac cgcattgaag gtggcacccc gtccgttatt
     1621 ggcatttacc agcgcccatc ctctgcaatc gtcgatgtga ttgccgacag catccgcatc
     1681 acacatcgcc atgccgacac agaagacacc gtgctggaaa ttcagcggct ggctacagtc
     1741 tgccgcgaca ccctgaatgg catgacgaag taaatcacta tgacggcaga gtacatcagg
     1801 gactggcaac aaccgcgcca cgcagtgggg cgtgaaggaa cggggatccc cgctcctgaa
     1861 tccgcgcttt cctcctggct ggatgcccac cgggcagaga acgagcgccg ccaggaaatg
     1921 gctgatgcgg cgttctccgc cacgccactg ggcaacctga ttaataaaag cctggacgca
     1981 caggaaaaac aggacaaaac catcacactg gcaggagacg ccagaaaaca ggcacgcggc
     2041 gcggtggatg aagccatggc ctcgctgcgc ctgctgccgt cctatctgcg cgatccgctt
     2101 attcgccacc tctccttcct gcgcaaaaaa caggaagccg atcgccggaa aggcaaaaag
     2161 agctggcagg cggaacgcta tgcacgcgga accctgcgca aaatattcga acgtctggat
     2221 cgcactgacg gacactggct gacaccgggt tatcgctccc ttgccggacg tgaacgcctg
     2281 gacgatttgc tttacctgcc gcaactcaac aaacaccaga tacagacgct ggccaccatg
     2341 acggcggcga tgttcagcag caccttcgaa aaactctgcg atggttttgg cgcgactgat
     2401 ggcgagctga ccatggatgt aacgctgaag gcgtatcaga tgctggcccg catggcgtta
     2461 cacttacacg ccatgcctcc gcattatgac gcactgacaa cagacaaaga ccggaggcac
     2521 gaaccggaca cagaactgct gccgggcgca atccttcgcc tgacctgtgc ggaatggtgg
     2581 aaacgcaaac tgtggctgtt acgttgcgag tggagagaag aacaacttcg cgccgcctgt
     2641 ctggtttcca gaaaaacatc gccctatctg agccaggacg cgttaagcga gtttcgcgca
     2701 cagcgcgaga aaacacgcga tttcctgaaa agtttcatgc tggaaaatga agacgggttc
     2761 acgattgatc tcgagacggt gtattacgcg ggagtaagta acccggttca ccgtaaggca
     2821 gaaatgatgg ccaccatgaa ggggctggaa cttctggccg aagcccgtgg cgacaaagcg
     2881 gtgtttctga ctgtcacctg cccgtcaaaa taccacgcca caacggagaa cggtcatccg
     2941 aatcccaaat ggaacggggc cacaatgcgc gactccagcg attacctggt taacacgttt
     3001 tttgcgacgg tccgcaaaaa actgaaccgc gacggcctgc gctggtatgg catccgcacg
     3061 gcggagcctc accatgacgg caccgtgcac tggcatatga tggtctttgc tcatccggaa
     3121 gaaatcgaca ccattgtgtc ccacacccgc gatattgcca ttcaggaaga tcgtcacgag
     3181 ctgggcgatg acataactcc gcgctttaag gcggagtatg tagacggctc aaaaggcacg
     3241 ccaaccagct acatcgccac ctacatcggg aaaaacctgg acagccgcgc cgtggatggc
     3301 atcgacccga aaacgggcaa gccacgcgtt gaccacgaaa ccggaaaatc aatggccgag
     3361 agcgtggaac gcgccatcgg ctgggcgcgc cttcaccggg tccgccagtt ccagttcttt
     3421 ggcatcccct cccgccaggt atggcgtgaa ctgcgccgcc ttgccagcca gatggcacgc
     3481 aacccggaag gcccgcaacg gctgaaggat gacgcaatgg atgcggttct tgccgccgct
     3541 gatgccggat gttttgccac ctacatagag aaacagggcg gcgtacttgt tccacgcaaa
     3601 gactacctga ttcgcaccgc ctacgacctc gccgatgagc tgaacgatta cggcgaacag
     3661 agcgtacaga tttacgggat ctggtcacca ctcatcgggg aatcctcccg tgtgtgcacg
     3721 catccggata actggaagct ggtaagacgc aaaccggaag cggaagacag cgcccgcgaa
     3781 aatggttttg accttcaggg cggccctgcc gccccttgga ctcgtggcaa taactgtccc
     3841 cgtgtacagg aaacggacaa caacgggaca gaacagccgg aagaacggcc agcaccgtgg
     3901 ccgcagctcc ctgacggcgt tgatgtggat gaatggatgc gctcactgaa acggcacgaa
     3961 cgccgggcgc tgatgcgttc gcttcgtgac aaacaggcaa aaaacagcag tgatgaaatg
     4021 cagagctgga cacagagccg caaacagcag cggcctttgc ctgataacca cgaattactc
     4081 gctaaagaat ggcgggagtc tgctgaatct ctcggcctgc atatcggtga acaacagatg
     4141 cagcacctgt tacggggcgg cagtctgtac gttgacgaca gcatcattgc accgcaggga
     4201 tttgaaattg tacgcaaacc ggatacccgc ccggacagcc gaatcacgta gctctggcag
     4261 cgcctgagcc gtaatcatgg cgtaagcagc acggagatcc gccataaccc ggtcgccagc
     4321 tatctgacac agctgggggc atcagaccct gaagccgccg cacgcctggc atccacactt
     4381 cagcaggacc agaacaccat gaaaacaccc gttaccgtgc tttctgacat gctgcgcgcc
     4441 atccgcgacg cagagcacgc acagagaatc agtgaaacca ctgaacgcgc ccgccgcaaa
     4501 gcagacctgc tgcggggtgg cctgaccagt ggaaacaaaa aacagacaga aacgggactc
     4561 acgaatcccg taaatgagca aaaaacgcgc agcgatatat gaagcgcgca caaaacaggc
     4621 aaaaacggga tttcagaatc ccgtaaacga ttaattaatc aacataagga aaagcgacat
     4681 gaaaatttgt atcgacgacg gctccaccaa catcaagctg gcatggactg agaacggcga
     4741 acgccgcaac gccatcagcc cgaacagctt caagtcggaa tggtctgcgc cgttcggtgg
     4801 catgcagccc gcgaactaca tgcttgatgg cgtgcgctat ggttttgatc cggtcagcga
     4861 tcgctttgtc cagacaaccg acacgcaata ccagtacagc gatgtaaatg tcattgccat
     4921 tcatcacgcg ctggtcaaat caggcatcac gccacaggag gtggatgtgg ttgtcaccct
     4981 gccactgagc gagtatttcg acacaaacgc acagccggac atggccaaca tcaaccgcaa
     5041 aaaagcgaac gttatgcgcc cggtggagta ccagaacggc gaagcattca ctatccgtaa
     5101 cgtgcgggtt atgcctgaat ccattccggc tggctttaag gcactggctg acatgagtcc
     5161 gtttgaatcc ctgctgattg tggatttggg cggaaccacg ctggatgtgg caaaggttca
     5221 ggggcaactg gcaggtatca gccaggtgtt ttgcgatcca cacgtaggcg tttctctgat
     5281 ggccgatgcc gtactgtcgg tgatggccac taacggtatg cgcaccagtc accacatcgc
     5341 caataccatt atcgaacatc gccatgatga agcctggctg cgccagcaca tccacaatga
     5401 cgcgcattac gccagcctga tggcggttat tcgtgaaaag gaagaaacac tgaaacaacg
     5461 cgtgatccgc gcgctggcgg gtttttcggg ttacgggcgg gtgatggttg tcggtggagg
     5521 ggcggagatt gtggcacccg ctatccgcga agcctgcgga gttaatgcga ctttcatcgc
     5581 ggacggggtg ccacagtttg ctctggttaa tgggctgtac gcaatggaca aggagtaaac
     5641 caatgacgac accaaccaga cggataagtt tctatctgaa gcctgcggcc gtcaagaacg
     5701 aaagcgaagc atgcgcctgg ctgggcagcc ttacaccaga agcccgcaaa agcggccaac
     5761 gcgtggcttt tctggccggg ctggcacttc tgaaaatgaa tccagcagag gcttaccgac
     5821 tggctgcatg ggctgacgat gaggcgttat cggtgacaca aaccaggaca gaacgccccg
     5881 tgccacagcc agtaccaacc gcacagataa ccagtcagat agcagggaat atccgggcat
     5941 tatttcctga atgacgaatt tgacggcttt taattttatc ggggtgcact gtcgccccgc
     6001 acgaggaaaa cacgatgaaa aaatacgaat acatgctaat gaaagaagca ctgcgggctg
     6061 gtctggctcc ctgtcctgta tccgtagaag ctgttgttgg gaacacgatt ttccgaacgc
     6121 tgcgaaatcg cgtatccgtg aagatggttc accaaccacc caaatctacc agcatgatct
     6181 atctgccgct gcccgaggct atcaaactta tgcgttcgct gaaatttgcg atttggatgc
     6241 agacacaaat caacaaagcg gtgcaaaaca ccttcgttaa gagactaata ccggagcttg
     6301 ctgccagatg tgcaaaatgt accagacccg cacttcaatt tgatctgtca ttgattcaac
     6361 aagctacgga ggcagaactg taccgcctgt cacctatccg tgatgctcac acggaagcat
     6421 cagaaatgtt ccggctcatg tttcaattta attttgccgt tcgacgcaca cgcgttcagg
     6481 tcaatcgcat gatgcaggag caggaagagt taacacagta caaagggggc agccagtgag
     6541 taattcagca cgactacagc ttggtttttc accgctatca aaaaccatcg tattggcaaa
     6601 aatgcgtgac ttaggagacg gaacaaaacg ccgcgtagga aatgatcgcg gtcgcgatgt
     6661 aaccaacgag gccgctcaac tcgtttggca tctagtcatg gcagaaggcg gtgaaattaa
     6721 ttgggagctg gatgatggtt ctcgtatgat tttgagggcc gagaagagta aatagctcaa
     6781 gggggattaa tgctaaatga tgatgaagaa gagctgctta tgcaggaatg gtcgctcggt
     6841 gattatgata atggtgaaaa tgggtgcccg cattgtggcc gttatcgttt atgtatctgc
     6901 cagaatggaa aacacagatg tgaaaagtgc aattggtctc cggaacttaa tgactacgtg
     6961 ccgatcgagt ggtagaggta agtcacacta ggtgtttttt agttgatttt ataaatgcac
     7021 aatagtgcac aaatttgcac aatttttttg aacgactttt tgcccttccg gcccgcgtgg
     7081 cggctggatc cgtcaaggat ccgtgcatgc acaaaaaaac gcgttttttc tgcgcgcagg
     7141 tgacggggga acagcccgcg tttcaggggg taaatagcat tccctgaacg atgtcgcaga
     7201 gatacaacag aatggctgta tttctcacgc tgagcgtgaa aaagacgtga gggcttttga
     7261 tttgatgggg tgaaaggtaa ggccgtcaaa atcgcactga gacggcgaga atatgcagtc
     7321 aacgaggtgg gattgcgtaa gagtctgact gtcgatgatg gcaatcagca ggaaagcgtc
     7381 gtgaaattat ctgactgata caggagctgg agagtcgggg cataaatttt ttatgccccg
     7441 gcgaagcagc agacaagcga agcgcgtcag gatgtgggct gggtgtctaa cagtgcgtaa
     7501 gggttaaagc ggatcacctc ttcgccaagc cagtcattga tgtgcttcat ggcctccatg
     7561 acgggcatca gctcgttaat tgcgtaaacc cgcgcggcct tctccacatc accaaacgca
     7621 cttttttcgc ccggcatcgc ccccatcagt tgcggcggaa cgcggtgcgc agccagcaca
     7681 tcatcacggg atgccgcctt aacattcatg aactcatcct ttgcggtgat ctgctggaac
     7741 ggcaaaattt gcaccccctc tttgcccccg ttgggcgcat gaatgagcac gtttttaaac
     7801 gcaccaccac cacgtgcccc ctgtagcgtt tctttcaggg agtccatgct ttcgcggttt
     7861 acctgcgctg caccgatgta gatgatgcac ccggcgtggg atccgttgtc gtagtacagt
     7921 tttctgaaca tgtccgccga atgagaaagg ctggccgaga gtaatgcgcc aagatattcc
     7981 ggcatgccgt agatttcctg gttaatatcc ggattcatca ggtggcacac tttgccagga
     8041 cgaaactgaa acgcatcctt gccatcctgc acataccacc atgattcaag atcgcttccg
     8101 cgtcgcatgt atttcgccag ggcgtgccgt aatttaagcg gttcgccgag catattgctc
     8161 cgaagctcaa ggaatgcgtt accgaacaca aaccagtcca gcgccagcgc cgagaaatcc
     8221 tgccgggaaa gcagcgggtg cggaatatag cagccgagca atacattgcg cttaaagtaa
     8281 agcgcagact gatgccagga cgtttgccgg gcagctcttg ccagaccgta ccagtccacc
     8341 ggggtttcat accaccgccc gttatcagca cagtacatat tgtccagcag gtcatgcccg
     8401 gtcaggcggt aaggaccatc aaatgtgaat gcactgagcg acgattcttt cctgagcgca
     8461 tcagcgagat caatgcgtga actcatgcgc acttttttat tttttctgct catcagaact
     8521 ccataaccgt gaaacgctcg ttttctcctt cgccgccaat cggttcgtta atgacagcaa
     8581 gcatggttgc ccacgcaagg tcgccgtggc tgatccccct cgcgcggtcc gtttcgtaag
     8641 tgataaaacc gcccggtgtt ttcaccttac gcacggcgtt aaaggccgca accagctcgc
     8701 gttcggcgcg atcgtattcc caccgcccgg cacgcattat ttgcagcatt ttcagtacca
     8761 gcgaccgttt tgatgacagc gtgaaggtgt aagggatggc ggcgggaaaa aaccgtttca
     8821 ctatctgata gacggcatcc ccgtgcccgc ccgtcgcatc aatgccgatg tgttccacgt
     8881 tgtagcgata cgtgaactct tcaataactc tggcctgctc ttcaaattcc agcccctgaa
     8941 cgcgtcgcgt ctccaccgtt ctgaaacgac caccaggaac agccggagga accaccacgg
     9001 acacagcgcc gctgtcgccg ttaccactgc tgccgtttgc gtcataccca atccataccg
     9061 gacgattccc catcgggcgg ggggcaaagg gtttccagtc tttccagtcg tcgtatccgt
     9121 caacaccgca gccaatcagg atattcaggt taaatgccga ttccccttcg cggacaaact
     9181 cacacatata gagattgcgg aactcgtctt cggtgttttc atcacgaatt tcatcaatat
     9241 cggtgtgttt ccagccgtga ttaaccacat cttccagcgt gacaatttgc cgccacgtcc
     9301 ggtcagggca gataagcccg ttatgcagcg ttttccagtc cacagaaaaa cgctggcgtt
     9361 tatgcgcggc ctttttctcg ttccagcggt cgccgttcca gtaggcgtat gcctcgtgcg
     9421 tttcggtgga tggcgtggag aagcaggtgc gccgcagtcc gctgagggtt gccatagcgc
     9481 cagccacctt gcgcagttca gcaaagcgac tgacccagaa aaattcatca aaataaaaat
     9541 tgcccgtgta agattgcgcc gtcgcagcag aagtgccgag aaaatgcatc tctgcgccgt
     9601 tggagaggat gattttatcg ccccctttca gctccacatc gacttcagcc gcggccttct
     9661 gaataatgct tttaaactgg aacgcctgac gacgcgacgc agacaaaaaa atctggttac
     9721 gctggtaagg ttgtgccaca tcgtcacgca gcgccatcag cagtgcttcc tgtgcaaaat
     9781 accaggtcgc cccaatctgt cgggatttca ggatcatcct gttacgtatc ccggcttccc
     9841 tgcaaagcgt cagggagtca aaccagcccc gctgatgcca ctccagcctg ctgatgattt
     9901 tttcccgcag tgcggcaatc tgttccggcg tgaaatgatt tttgagtttt ttcgcccggc
     9961 ctttctttcc tgtggccgtc gcatccggct ggccatcatg cagtttttta agctgccggg
    10021 tcagcaggtc tatttcctta aagtcaccac ctgttttatt ctgtttttca gtaagctgga
    10081 tgaggcgcgc atcgatggac tgcgtgacac gctgcacggg tggcgtttca tcccactggt
    10141 cgcgtttttt ccacgcataa atcgtgttcg ggtttattcc catcagacgt gatatttctg
    10201 cgggcggata accctgccag taaagttgcc gcgcacgctg gcgcacaaaa gcgtcctgaa
    10261 tcattgctcc ccctgagtaa ttacaggaag attacccgcg cgcgaaaccg ttctccttaa
    10321 ccccctgttc tggccgtttt cttacaacaa aagccctttg tatcagcctg ttacgctttg
    10381 ccatcatgac tgaagaacca gtcagagggg caaaaactat ggctaatgaa aaaaagacat
    10441 cccgcaaaaa gtttcgcgtg gctgtctccg gatcaactgt tgatggccgt gaaatcagtc
    10501 cggtgcatct gcgtgaagcc gccgagaact tcaacccgga tgtttacgct gcccgcgtga
    10561 acgttgagca ctatctctcg ccatgcccgt caagcgaatt ttccgcaatg ggcgatgtca
    10621 ccgcactgag tacggaagac attacggaag gtccgctggc cggacgtact gcgctgtatg
    10681 cagaaatcga accgaccgag cgcatgaagc agcttgtcgc ggacggcaag aaaatctatt
    10741 ccagtatcga actgcacccg cagttctccg ttaacgggcg cgcctatctg gtcgggctgg
    10801 cgatgaccga caccccggca agcctgggca ctgagcgcct gaaattcacg gcacagcaac
    10861 gtcaggcggt gatgacgttc aacagtatcc agggtgaagc accgcttatc tccgaagcca
    10921 tcgagtctga aatcatcgaa atggcagaac aacgccagga agaaggcacc cagtggttta
    10981 accgcgtaat ggggattatt ggtcgtggcc gcaaagcgga tgacgccagt ttctcccgta
    11041 ttcaggaagc ggtggaaagc gtcgcaacgt cacaggccga cattatcgac cgttttaatg
    11101 cgctggaaac acgccatcag caggacagcc agaaaatcac tttactgacc acggagctga
    11161 cagcactgaa ggaaaaactg cgcacgcagg acggcgatcc gcagaaccgg ttcaccgcaa
    11221 cgggcgcagc ctccgaccag ctggctgact tctgataaga caaaggagca aattttttat
    11281 gaatctggtg atgtcagata ttacccgcaa caagctgggt tgctatatgg cgcagcaggc
    11341 gtcgcttaac aatatcccgg tttctgcact ggtatcgcga tttaccgtgg aacctgcggt
    11401 gcagcaacgt tttgaaaacg cctcaaagga tagcaccgaa cttacaaaaa aaattaacgt
    11461 gatcggcgtg accgaccaga aaggcgaaaa aatcctcctg gacaccacag gaccgattgc
    11521 acgcacaaat accagttatg acggaacaaa acgccgtaac ccgaataacg tggttgatct
    11581 gaaaaaccgc aaataccagt gcgaacaggt gaactacgac acgtttattt cgtatccgca
    11641 gcttgatgcc tggtcggcac acagcgattt tcagtcccgc atcagcgcac agattgcccg
    11701 acaggtggca cttgaccgca tcatgatcgg tttcaacggc acatctcacg cggatgagtc
    11761 caacttcagc accaacaagc tgcttcagga cgttaacgtg gggtggctgg agcacatcag
    11821 aaccaatgcc agcgagcgcg taatgaatga cgtgacgctg acttcccgca acatggacaa
    11881 caccgtggcg cacgcgggta aatacgcgaa ccctgatgca ctggtacagg acgcgcgctc
    11941 atctctgctg gatgaatggc acaaggaagc tgacgacctc gtggtgatta tggggcgcaa
    12001 cctgtttaac tcgctgcgtc tgccagtgct gaacagcatc agcggccaga atcccaatgc
    12061 ggaattactt gccgggcagc tcatcctgtc atcgcgcacc attggcgggc tgggtgtgtt
    12121 ccttgcaccg ttcttcccgg atgcaacgat gctgatcacc tcattcaaca acctgtcgat
    12181 ttactggcag aa
//
LOCUS       DAZRUF010000121        12113 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZRUF010000121 DAZRUF010000000
VERSION     DAZRUF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.121"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            269..1378
                     /gene="livK"
                     /locus_tag="ACHXOL_002639"
     CDS             269..1378
                     /gene="livK"
                     /locus_tag="ACHXOL_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR6809589.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1426..2352
                     /gene="livH"
                     /locus_tag="ACHXOL_002640"
     CDS             1426..2352
                     /gene="livH"
                     /locus_tag="ACHXOL_002640"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR6809590.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2349..3626
                     /gene="livM"
                     /locus_tag="ACHXOL_002641"
     CDS             2349..3626
                     /gene="livM"
                     /locus_tag="ACHXOL_002641"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR6809591.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3623..4390
                     /gene="livG"
                     /locus_tag="ACHXOL_002642"
     CDS             3623..4390
                     /gene="livG"
                     /locus_tag="ACHXOL_002642"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR6809592.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4392..5105
                     /gene="livF"
                     /locus_tag="ACHXOL_002643"
     CDS             4392..5105
                     /gene="livF"
                     /locus_tag="ACHXOL_002643"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR6809593.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5389..6453
                     /gene="ugpB"
                     /locus_tag="ACHXOL_002644"
                     /pseudo
     CDS             5389..6453
                     /gene="ugpB"
                     /locus_tag="ACHXOL_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            6551..7438
                     /gene="ugpA"
                     /locus_tag="ACHXOL_002645"
     CDS             6551..7438
                     /gene="ugpA"
                     /locus_tag="ACHXOL_002645"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR6809594.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7435..8280
                     /gene="ugpE"
                     /locus_tag="ACHXOL_002646"
     CDS             7435..8280
                     /gene="ugpE"
                     /locus_tag="ACHXOL_002646"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR6809595.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8282..9352
                     /gene="ugpC"
                     /locus_tag="ACHXOL_002647"
     CDS             8282..9352
                     /gene="ugpC"
                     /locus_tag="ACHXOL_002647"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR6809596.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9349..10092
                     /gene="ugpQ"
                     /locus_tag="ACHXOL_002648"
     CDS             9349..10092
                     /gene="ugpQ"
                     /locus_tag="ACHXOL_002648"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR6809597.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10079..10519)
                     /locus_tag="ACHXOL_002649"
     CDS             complement(10079..10519)
                     /locus_tag="ACHXOL_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR6809598.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10639..>12113
                     /gene="ggt"
                     /locus_tag="ACHXOL_002650"
     CDS             10639..>12113
                     /gene="ggt"
                     /locus_tag="ACHXOL_002650"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR6809599.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGMGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVL"
BASE COUNT         2636 a         3106 c         3482 g         2889 t
ORIGIN      
        1 cccctgaatg gtagtgaatt attccgccct ttgtgccgtt attttatgct gacaaaggca
       61 cttttttctg tttatctatc aataaattca gaatattatc tgttcttaat cgactgaaaa
      121 atagggattt taatcactgt tatcacaaaa tactgcgcta aacccttaat cagacaggca
      181 aaaacagtgc agtataaaaa aagaacagtc tgatttgtta acacataaaa acaaagcaac
      241 acaacatcac gaatggggat ttttgactat gaaacggaat gcgaaaacta tcatcgcagg
      301 gatgattgca ctggcaattt cacacaccgc tatggctgac gatattaaag tcgccgttgt
      361 cggcgcgatg tccggcccga ttgcccagtg gggcgatatg gaatttaacg gcgcgcgtca
      421 ggcgattaaa gacattaatg ccaaaggggg aattaagggc gacaagctgg ttggcgtgga
      481 atatgacgac gcctgcgacc cgaaacaagc cgttgcggtc gccaacaaaa tcgttaatga
      541 cggcattaaa tacgttattg gtcatctgtg ttcttcttct acccaacctg catcagatat
      601 ctacgaagac gaaggtattc tgatgatctc gccgggagcg accaacccgg agctgaccca
      661 acgcggttat caacacatta tgcgtactgc cgggctggac tcttcccagg ggccaacggc
      721 ggcaaaatac attcttgaga cggtgaagcc ccagcgcatc gccatcattc acgacaaaca
      781 acagtatggc gaagggctgg cgcgttcggt gcaggacggg ctgaaagcgg ctaacgccaa
      841 cgtcgtcttc ttcgacggta ttaccgccgg ggagaaagat ttctccgcgc tgatcgcccg
      901 cctgaaaaaa gaaaacatcg acttcgttta ctacggcggt tactacccgg aaatggggca
      961 gatgctgcgc caggcccgtt ccgttggcct gaaaactcag tttatggggc cggaaggtgt
     1021 gggtaacgca tcattgtcga atattgctgg cgatgctgcc gaaggcatgt tggtcactat
     1081 gccaaaacgc tatgaccagg atccggcaaa ccagggcatc gttgatgcgc tgaaagcaga
     1141 caagaaagat ccgtccgggc cttatgtctg gatcacctac gcggcggtgc aatctctggc
     1201 gactgccctt gagcgtactg gcagcgatga gccgctggcg ctggtgaaag atttaaaagc
     1261 taacggtgca aacaccgtga ttgggccgct gaactgggat gaaaaaggcg atcttaaggg
     1321 atttgatttt ggtgtcttcc agtggcacgc cgatggttca tccacggcag ccaagtgatc
     1381 atcccaccgc ccgtgaaatg cgggcgggtt tagaaaggtt accttatgtc tgagcagttt
     1441 ttatatttct tgcagcagat gtttaacggc gtcacgctgg gcagtaccta cgcgctgata
     1501 gccatcggct acaccatggt ttacggcatt atcggcatga tcaacttcgc ccacggcgag
     1561 gtttacatga ttggtagcta tgtctccttt atgatcatcg ccgcactgat gatgatgggc
     1621 attgataccg gctggctgct ggtggcggcg ggattcgtcg gcgcaatcgt cattgccagc
     1681 gcctacggct ggagtatcga acgggtagcc tatcgcccgg tgcgtaactc taagcgcctg
     1741 attgcgctca tctcggcaat cggcatgtcc atcttcctgc aaaactacgt tagcctgacc
     1801 gaaggttcgc gtgacgtggc gctgcccagt ctgtttaacg gtcagtgggt ggtggggcat
     1861 agcgaaaact tctctgcctc tattaccacc atgcaggcag tgatctggat tgttactttc
     1921 ctcgccatgc tggcgctgac gattttcatt cgttattccc gtatggggcg cgcctgccgt
     1981 gcctgcgcgg aagatctgaa aatggcgagc ctgcttggca tcaacaccga ccgggtgatt
     2041 gctttgacct ttgttattgg cgcggcgatg gcggcagtgg ctggcgtact gctcggtcag
     2101 ttctacggcg tcattaaccc ctatatcggc tttatggccg ggatgaaagc ctttaccgct
     2161 gcggtgctcg gtgggattgg cagcattcca ggggcgatga ttggcgggct gatcctgggg
     2221 attgcggagg cgctctcttc tgcctatctg agtacggaat ataaagatgt ggtctcattc
     2281 gccctgctga ttctggtgct gctggtgatg ccgaccggta ttctgggtcg cccggaggta
     2341 gagaaagtat gaaaccgatg catattgcaa tggcactgct ctctgccgcg atgttctttg
     2401 tcctcgctgg cgtctttatg ggcgtgcaac tggagctgga tggcaccaaa ctggtggtcg
     2461 acagcgcttc ggatgtccgt tggcagtggg tgtttatcgg cacggcggtg gtctttttct
     2521 tccagctttt gcgacctgct ttccagaaag ggctaaaaag cgtttccggg ccgaagttta
     2581 ttctgcccgc gattgatggc tccacggtga agcagaaact gttcctcgtg gcgctgctgg
     2641 tgcttgcggt gtcgtggccg tttatggtgt cacgtgggac ggtggatatc gccactctga
     2701 ccatgatcta cattatcctc ggtctcgggc tgaatgtggt tgttggtctt tctggtctgc
     2761 tggtgctggg gtacggcggt ttttacgcca tcggcgctta cacttttgcg ctgctcaatc
     2821 actattacgg tctggggttc tggacctgtt tgccgattgc cggactgatg gcagcggcgg
     2881 cgggcttcct gcttggtttt ccggtgttgc gtctgcgcgg tgattatctg gcgatcgtta
     2941 ccctcggttt cggcgaaatt gtgcgcatat tgctgctcaa taacaccgaa attaccggcg
     3001 gcccgaacgg tatcagtcag atcccgaaac cgactttctt cggccttgag ttcagccgta
     3061 ccgcccgtga aggcggttgg gacacgttca gtaatttctt tggcctgaaa tacgatccct
     3121 ccgatcgcgt catcttcctg tatctggtgg cgttgctgct ggtggtgcta agcctgtttg
     3181 tcattaaccg cctgctgcgg atgccgctgg ggcgtgcgtg ggaagcgttg cgtgaagatg
     3241 agatcgcctg ccgttcgctg ggcttaagcc cgcgtcgtat caagctgact gcctttacca
     3301 tcagtgccgc gtttgccggt tttgccggaa cgctgtttgc tgcgcgccag ggctttgtca
     3361 gcccggaatc cttcaccttt gctgaatcgg cgtttgtact ggcgatagtg gtgctcggcg
     3421 gtatgggatc gcagtttgcg gtgattctgg cggcaatttt gctggtggta tcgcgcgaat
     3481 tgatgcgtga tttcaacgaa tacagcatgt taatgctcgg tggtttgatg gtgctgatga
     3541 tgatctggcg tccgcagggc ttgctgccca tgacgcgccc gcaactgaag ctgaaaaacg
     3601 gcgcagcgaa aggagagcag gcatgagtca gccattatta tctgtgaacg gcctgatgat
     3661 gcgcttcggc ggcctgctgg cggtgaacaa cgtcaatctt gaactgtacc cgcaggagat
     3721 cgtctcgtta atcggcccta acggtgccgg aaaaactacg gtttttaact gtctgaccgg
     3781 attctacaaa cccaccggcg gcaccatttt actgcgcaat cagcacctgg aaggtttacc
     3841 ggggcagcaa attgcccgca tgggcgtggt gcgcaccttc cagcatgtgc gtctgttccg
     3901 tgaaatgacg gtaattgaaa acctgctggt ggcgcagcat caacaactga aaaccgggct
     3961 gttctctggc ctgttgaaaa cgccttcttt ccgtcgcgcc cagagcgaag cgctcgaccg
     4021 cgccgccacc tggcttgagc gcattggttt gctggaacac gccaaccgtc aggcgagtaa
     4081 cctggcctat ggtgaccaac gccgtctgga gattgcccgc tgcatggtga cgcagccgga
     4141 gattttaatg ctcgacgaac cggcggcagg tcttaaccca aaagaaacca aagagctgga
     4201 tgagctgatt gccgaactgc gcaatcatca caacaccact atcttgttga ttgaacacga
     4261 tatgaagctg gtgatgggga tttcagaccg aatttatgtg gtcaatcagg ggacgccact
     4321 ggcaaacggt acgccggagc aaatccgtaa taacccggac gtgatccgtg cctatttagg
     4381 tgaggcataa gatggaaaaa gtcatgttgt cctttgacaa agtcagcgcc cactacggca
     4441 aaatccaggc gctgcatgag gtgagcctgc atatcaatca gggcgagatt gtcaccctca
     4501 ttggcgcgaa cggggcgggg aaaaccacct tgctcggcac gttatgcggc gatccgcgcg
     4561 ctaccagcgg gcgaattgtg tttgatgata aagacattac cgactggcag acggcgaaaa
     4621 tcatgcgcga agcggtagcg attgtcccgg aagggcgtcg cgtcttctcg cggatgacgg
     4681 tggaagagaa cctggcgatg ggcggctttt ttgccgaacg cgaccagttc caggagcgca
     4741 taaagtgggt gtatgaactg ttcccacgtc tgcatgagcg ccgtgttcag cgggcaggca
     4801 ccatgtccgg cggtgagcaa caaatgctgg cgattggtcg cgcgctgatg agcaacccgc
     4861 gtttgctgct acttgatgag ccatcgcttg gtcttgcgcc gattatcatc cagcaaattt
     4921 tcgacaccat cgagcagctg cgcgagcagg ggatgactat cttcctcgtc gagcagaacg
     4981 ccaaccaggc gctaaagctg gcggatcgcg gctacgtgct ggaaaacggc catgtggtgc
     5041 tctccgatac tggtgatgca ctactggcga atgaagcggt gagaagtgcg tatttaggcg
     5101 ggtaataaca cgttgattga taaggagtca aaagactcct ttgagacagg tgacaaatgt
     5161 aaaattgcct gatgcgctgc gcttatcagg cctactgggt gagttgcaat atattgaatt
     5221 tgcacgatct tgtaggccgg ataaggcgtt tacgccgcat ccggcatgaa acgatgagta
     5281 atctgtagag tttgattcag accttctatt tttccgctta tccgtgcccc atctcccatt
     5341 ttccctcacc cacgccgtca ccgccttgtc atctttctga caccttactt gcaggtttat
     5401 gaagttggca ccgccaccat gatggcatcg aaagccatta aaccggtata tgacgtgttt
     5461 aaagaggcag ggattcaatt cgatgagtcg cagtttgtgc cgacggtttc cggttactac
     5521 tccgacagca aaacgggcca cttactctcc cagccattca acagctcgac tcccgttctc
     5581 tattacaaca aagacgcctt caagaaagcg gggttagacc cggaacagcc gccgaaaacc
     5641 tggcaggatc tggcggactc ttccgcaaaa ctgaaagcct ccggtatgaa gtgcggctac
     5701 gccagcggct ggcagggctg gatccaactg gaaaacttta gcgcctggaa cggtctgccg
     5761 tttgccagca aaaacaacgg ctttgacggc acggacgcgg tgctggagtt caacaagccg
     5821 gagcaggtga aacacattgc catgctcgaa gagatgaaca agaagggcga cttcagctac
     5881 gtcggtcgta aggatgaatc caccgagaag ttctataacg gtgattgcgc gatgaccacc
     5941 gcctcttccg gttctctcgc taatattcgt gagtacgcca aatttaacta tggcgtaggc
     6001 atgatgcctt acgacgccga tgcgaaagat gcaccgcaaa acgccattat cggcggggcc
     6061 agcctgtggg tgatgcaggg caaaaataaa gaaacgtata ccggcgtggc gaagttcctc
     6121 gacttcctcg cgaagccaga aaacgctgcc gagtggcatc agaaaaccgg ttatctgcca
     6181 atcaccaaag cagcgtatga cctgacccgt gagcagggct tttatgagaa aaacccaggg
     6241 gcggataccg cgacgcgtca gatgctgaat aagccgccgt tgccgttcac caaagggctg
     6301 cgtctgggca acatgccgca gatccgcgtg attgtggatg aagagctgga gagcgtgtgg
     6361 accggtaaga agacaccaca gcaggcactg gataccgccg ttgagcgtgg aaatcagttg
     6421 ctgcgccgct ttgagaaatc gacgaagtct taatcagtgt aatgtcggat gcgtttcgct
     6481 tatctgacct ggcatcgcgt gtaggccgga taagcgaagc gcatccggca cagttcagga
     6541 attaaccgta atgtcatcat cccgtccggt gttccgctcg cgctggctgc cttatctgct
     6601 ggtcgcgccg cagctcatca tcaccgttat cttttttatc tggcctgcgg gcgaagcgtt
     6661 gtggtactcg cggcaaagcg tcgatccgtt tggtttctcc agccagtttg tcggtctgga
     6721 taacttcgtc acgctgttcc atgacagcta ctatctagac tccttctgga cgacgataaa
     6781 attcagcacc tttgtcaccg tcagcggttt gctggtatcg ctgttctttg cggcgctggt
     6841 ggagtacatc gtgcgcggca gccgtttcta tcaaaccttg atgctgctgc cgtatgccgt
     6901 ggctcccgcc gttgccgccg tattgtggat cttcctgttt aaccccggtc gcgggctgat
     6961 cacccatttt ctcgctgagt tcggctacga ctggaaccac gcgcaaaaca gcggtcaggc
     7021 aatgtttctg gtggtgtttg cctcggtgtg gaagcaaatc agctacaact ttctgttctt
     7081 ctatgccgcg ctgcactcca ttccccgttc gttgattgaa gccgcagcta ttgacggcgc
     7141 agggccgatt cgccgcttct ttaagattgc tctgccgctt atcgctccgg tgagtttctt
     7201 cctgctggtg gtgaacctgg tgtacgcctt cttcgacacc ttcccggtga tcgacgccgc
     7261 cacgtccggc gggccggttc aggccaccac tacgctgatt tataagatct accgcgaagg
     7321 ctttaccgga ctggatctgg cttcgtctgc cgcgcagtcg gtggtgttga tgttcctcgt
     7381 catcgtgctg acggtggtgc agttccgcta tgttgaaagc aaggtgcgtt accaatgatt
     7441 gagaaccgtc cgtggctgac gatattcagc cataccatgc tgatcctcgg gatcgcggtg
     7501 atcctcttcc cgctgtacgt ggcgtttgtc gcggcgacgc tggataaaca ggccgtctat
     7561 gccgcgccga tgacgctcat ccccggcaca catctgctgg aaaatatcca caacatctgg
     7621 gttaacgggg taggcacgaa tagcgcgccg ttctggcgga tgttgcttaa cagctttgtg
     7681 atggcgttca gcattacgct cggcaaaatt accgtctcga tgctctcggc atttgccatt
     7741 gtctggtttc gttttccgct acgtaacctc ttcttctgga tgatttttat caccctgatg
     7801 ctgccggttg aagtacgtat cttcccgacg gtggaagtca tcgccaacct gaaaatgctc
     7861 gacagctacg ccggtttaac gctgccgctg atggcctcgg cgaccgccac gtttctgttc
     7921 cgccagttct ttatgaccct accggatgaa ttggtggaag ccgcgcggat cgacggcgcg
     7981 tcaccaatgc gtttcttttg cgacatcgtt tttccgttgt ccaaaactaa tctggcggcg
     8041 ctgtttgtga tcacctttat ctacggctgg aatcagtatt tgtggccgtt gttgattatt
     8101 accgacgttg atctcggcac caccgtggca gggatcaaag ggatgatcgc tacaggcgaa
     8161 ggcaccacgg aatggaactc ggtaatggcg gcgatgttgt taacgcttat ccctccggtg
     8221 gtgattgttt tagtgatgca gcgtgccttc gtgcgcggcc tggtcgatag tgagaaataa
     8281 gatggcagga ctgaaattac aggcagtaac caaaagctgg gatggcaaaa cccaggtcat
     8341 taaaccgctg acccttgatg tggcggatgg cgaatttatc gtgatggtcg ggccgtctgg
     8401 ctgcgggaaa tcgacgctgc tgcgcatggt tgccgggctg gagcgggtga cagaaggcga
     8461 tatctggatc aacgaccagc gcgtgaccga aatggagccg aaagatcgcg ggattgcgat
     8521 ggtgttccag aattacgcgc tttatccgca tatgagcgta gaggagaaca tggcgtgggg
     8581 gctgaaaatt cgcggcatgg gcaagcagca aattgccgag cgcgttaaag aagcggcgcg
     8641 cattctggaa ctggacggtc tgcttaagcg tcgtccgcgt gagctatccg gcggtcagcg
     8701 ccagcgtgtg gcgatgggcc gcgcgattgt gcgcgatccg gcggtgttct tgtttgatga
     8761 gccgctctct aacctcgatg ccaagctgcg cgtgcagatg cgtcttgaac tgcaacagtt
     8821 gcaccgtcgc ctgaaaacga cttcactcta cgttactcac gatcaggttg aagcgatgac
     8881 gctcgcccag cgagtaatgg tgatgaacgg tggcgttgcc gaacagattg gcacaccagt
     8941 tgaagtctac gaaaagcccg ccagcctgtt tgtggcgagt tttatcggca gcccggcgat
     9001 gaacctgctg acaggccgcg tgaataatga aggcacgcat ttcgaactgg acggcggtat
     9061 cgtgctaccg ctaaacggtg gctaccgtca gtatgccggg cgtaaaatga ctctcggcat
     9121 tcgcccggaa catatagcgc taagctcgca ggcagaaggt ggtgtgccgc tggtgatgga
     9181 cacgctggag atcctcggcg cagataacct ggcgcacgga cgctggggcg agcagaagct
     9241 ggtggtgcga ctggcgcatc aggagcgccc gacggcaggc agcacgctgt ggctgcattt
     9301 ggcggaaaat cagctgcatc tttttgatgg tgaaacagga caacgagtat gagtaactgg
     9361 ccttatcccc gcatcgtcgc tcatcgtggc ggcggtaagc tggccccgga aaacaccctg
     9421 gcggcaatcg acgtcggggc aaaatacggt cataagatga tcgaatttga cgcgaagtta
     9481 tcgaaagatg gcgagatctt cctgctccat gacgacaatc tcgaacgcac cagcaacggc
     9541 tggggcgtcg cgggtgaact gaactggcag gatttactgc gcgtggatgc gggcagttgg
     9601 tacagcaaag cgtttaaagg tgagccgctg ccgttgcttt cgcaggtggc ggaacgctgt
     9661 cgcgaacacg ggatgatggc gaatatcgaa atcaaaccca ccactggcac tggaccatta
     9721 acgggcaaaa tggtggcgct ggcggcacgc caactgtggg ccggtatgac gccgccgctg
     9781 ctgtcatcgt ttgagattga tgctttagaa gctgcacaac aggcggcacc ggaactgccg
     9841 cgtggtttat tgcttgatga gtggcgcgac gactggcgcg aactgaccgc gcggctgggc
     9901 tgcgtctcta ttcatctcaa tcataagtta ctcgataaag cgcgagtgat gcagttgaaa
     9961 gacgccggat tacggattct ggtttatacc gtcaataaac cccagcgcgc ggcagagttg
    10021 ctgcgctggg gcgtggattg catctgtacc gatgcgattg acgtgattgg tccgaacttt
    10081 acggcccaat agttttcaac ggaatgtcag gctgcggcgt gctgggctga ttcagcatgt
    10141 cgccgtttct cctttccggc agcatatgct gctgattcaa cgaactatcc ggattacggt
    10201 tgctgtttaa catcccgccg ttggtgttgg gcagcatttg ctgccgggcg ggatttcgct
    10261 cccccggctg tgactgcaac acccgctgag aattattgtt tatctggttt tctaaatgct
    10321 gctgttgcaa ctgagtttgc gttttcagtt gctgattcag catccctttt tgctggattt
    10381 gctgagtctg catctgggtt tgcatccgct gctggctggg tatctgatac cccggctggt
    10441 tagggttgtt cagagtatta atgggctgtg caaagccgac aaacggcagg agtgccgtaa
    10501 gaatcagaag tcgtttcatc gcgtatcctc ctctgaagat atcctttaag tttactcgct
    10561 tcccgacaaa acgatgatta attcagagtt atataccagg cttagctggg gttgcccctt
    10621 aatctctgga gaataacgat gataaaaccg acgtttttac gccgggtggc cattgctgct
    10681 ctgctctcag gaagttgttt tagcgccgcc gccgcgcctc ctgcgccgcc cgtctcgtat
    10741 ggtgtggagg aagatgtctt ccacccggta cgcgcgaaac agggaatggt agcgtctgtg
    10801 gacgccactg ccactcaggt gggggtggat atcctcaagg agggcgggaa tgccgttgat
    10861 gccgccgtgg cggtgggcta cgcgctggcg gtaacgcatc cgcaggcagg gaatctgggc
    10921 ggtggtggtt ttatgttaat ccgctcgaaa aatggcaata ccacggctat cgatttccgc
    10981 gaaatggcac ccgccaaagc gacccgcgat atgttcctcg atgatcaggg caacccggac
    11041 agcaaaaaat cactcacttc gcatctggct tccggcacac cgggtacggt agcaggtttc
    11101 tcgctggcgc tggataaata cggcaccatg ccgctgaaca aagtcgtgca gcccgcgttt
    11161 aaactggcac gcgatggttt tatcgttaac gacgcgctgg ctgacgatct caaaacctac
    11221 ggtagcgaag tgttgccgaa tcacgaaaac agtaaagcta tcttctggaa agagggcgag
    11281 ccgctgaaaa agggcgacac gctggtgcag gcgaacctgg caaagagcct ggagatgatt
    11341 gctgaaaacg gcccggacga attctataaa ggcacgattg cggaacagat cgcccaggag
    11401 atgcagaaaa acggtggctt gatcactaaa gaagatttag cggcctataa agcggtcgaa
    11461 cgcactccga taagcggcga ttatcgcggg tatcaggttt actccatgcc accgccatcc
    11521 tccggcggga tccatatcgt acaaatcctc aatattctgg aaaacttcga tatgaagaaa
    11581 tacggctttg gcagcgccga tgcgatgcaa atcatggcag aagcggagaa atacgcctac
    11641 gccgaccgct cggaatatct tggcgacccg gattttgtca aagtcccgtg gcaggcactg
    11701 accaataaag cctatgccaa atccattgcc gatcaaattg atatcaacaa agcgaagccg
    11761 tccagtgaga ttcgccctgg caagcttgcg ccttatgaga gtaatcaaac tacccattac
    11821 tctgtagtgg ataaagacgg taacgcggtg gcggtgacct atacgctgaa caccaccttc
    11881 ggtatgggca ttgtcgcggg cgagagcggt attctgctta ataatcagat ggatgatttc
    11941 tccgccaaac cgggcgtacc gaacatttac gggctggtgg gcggtgatgc caacgccgtc
    12001 gggccgaata aacgtccgct gtcatcgatg tcaccaacca ttgtggtgaa agacggtaaa
    12061 acttggctgg ttaccggtag tcctggcggg agtcggatta ttaccaccgt gct
//
LOCUS       DAZRUF010000122        12073 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZRUF010000122 DAZRUF010000000
VERSION     DAZRUF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.122"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            271..1041
                     /locus_tag="ACHXOL_002651"
     CDS             271..1041
                     /locus_tag="ACHXOL_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR6809600.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1032..1505
                     /locus_tag="ACHXOL_002652"
     CDS             1032..1505
                     /locus_tag="ACHXOL_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR6809601.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1613..2152
                     /gene="dnaT"
                     /locus_tag="ACHXOL_002653"
     CDS             1613..2152
                     /gene="dnaT"
                     /locus_tag="ACHXOL_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR6809602.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2155..2892
                     /gene="dnaC"
                     /locus_tag="ACHXOL_002654"
     CDS             2155..2892
                     /gene="dnaC"
                     /locus_tag="ACHXOL_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR6809603.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2941..3435
                     /gene="yjjA"
                     /locus_tag="ACHXOL_002655"
     CDS             2941..3435
                     /gene="yjjA"
                     /locus_tag="ACHXOL_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR6809604.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3689..5980
                     /gene="opgB"
                     /locus_tag="ACHXOL_002656"
     CDS             3689..5980
                     /gene="opgB"
                     /locus_tag="ACHXOL_002656"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR6809605.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6119..7141)
                     /gene="lgoD"
                     /locus_tag="ACHXOL_002657"
     CDS             complement(6119..7141)
                     /gene="lgoD"
                     /locus_tag="ACHXOL_002657"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR6809606.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7280..8194
                     /locus_tag="ACHXOL_002658"
     CDS             7280..8194
                     /locus_tag="ACHXOL_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR6809607.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8409..9770
                     /locus_tag="ACHXOL_002659"
     CDS             8409..9770
                     /locus_tag="ACHXOL_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6809608.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9819..11474)
                     /gene="tsr"
                     /locus_tag="ACHXOL_002660"
                     /pseudo
     CDS             complement(9819..11474)
                     /gene="tsr"
                     /locus_tag="ACHXOL_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(11593..12039)
                     /gene="hpaR"
                     /locus_tag="ACHXOL_002661"
     CDS             complement(11593..12039)
                     /gene="hpaR"
                     /locus_tag="ACHXOL_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR6809609.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         2932 a         3193 c         2947 g         3001 t
ORIGIN      
        1 ttaacttgtt aacattaaaa aacgtgcccg gttatctatt atcctgattc gtaaaggcca
       61 aacaataaat tcattatagt gataatattc atgtgatagt ttgtttgcgg cgagagataa
      121 ttcgcttttt atcaccgagc ttgtcccgat aaccaggatt aatcagcgca attttgatct
      181 taacctaccg caaaacaacc gtgcttcatg atacaaatgg attctcatta tctgaacaat
      241 acgcagcacg tctatgacaa agggcgagtt atgcaaactg agcaacagcg agccgtaaca
      301 cggctttgta tccagtgtgg attatttctt ttgcaacatg gtgcggaaag cgcgttggtt
      361 gatgagcttt cctcacgact gggtcgggcg ttggggatgg acagcgtcga aagttctatc
      421 tcttcgaacg ccatagtgct gacaaccatt aaagatgggc aatgcctgac atcgacacgt
      481 aaaaatcacg atcgcggcat taatatgcat gtggtgactg aagtccagca cattgtgatt
      541 ctggcggagc accatctgct ggattacaaa ggcgtagaga aacgatttag ccaaattcag
      601 ccattacgtt acccaagatg gctggtagcc ttaatggttg gcctttcttg cgcctgtttc
      661 tgcaaactca ataaaggtgg ctgggatggt gccgtcatca ccttctttgc cagtacggcc
      721 gcgatgtata tccgccagct gctggcacaa cgtcatcttc atccgcagat caacttttgc
      781 ctcaccgctt tcgccgccac caccatttcc ggattgcttt tgcaacttcc cactttcagc
      841 aatacaccca ctatcgcgat ggccgccagc gttctgctgc tggtgccggg atttccgttg
      901 attaatgccg tcgccgatat gtttaaaggc cacatcaata ccggactggc acgctgggcg
      961 atcgccagtc tgctgacact ggctacctgc gtcggcgtgg tgatggcact gacgatttgg
     1021 gggctacgcg gatgggtgtg atcgagtttc tgtttgcatt ggcgcaggat atgatcctcg
     1081 ccgccattcc agcggtcggc tttgcgatgg tgttcaacgt tcccgttcgg gcattacgct
     1141 ggtgtgcgct gcttggcgcg attggtcatg gttcacgaat gatcttaatg accagcgggc
     1201 tgaatattga gtggtcaacc tttatggcct ccatgctggt cggcaccatt ggtattcaat
     1261 ggtcgcgctg gtatctggcg catccgaaag tgtttaccgt ggcggccgtt atccctatgt
     1321 tcccgggcat atcggcttat accgcaatga tttcggcggt aaaaatcagc cagttaggtt
     1381 acagcgaacc gttgatgatt accctgttaa ccaactttct tacagcttca tcgattgttg
     1441 gtgcgttatc catcggtctt tccattcctg gattatggtt gtaccgcaag cgccctcgcg
     1501 tataaaattg cctcgcttaa agcacacgga tgagagacag cctcctctcc tccgtgtgtt
     1561 actataaaag ttatctccct tctcgttcat cgttccatat ttgagaaaca gtatgtcttc
     1621 cagagttttg accccggacg tcgttggtat tgacgccctg gtacacgatc accaaaccgt
     1681 tctggcaaaa gctgaaggcg gtgtggttgc cgtatttgct aacaatgccc cggcgtttta
     1741 tgccgtcacg cctgcacgcc tggctgaact gctggcgctg gaagaaaagc tggcgcgtcc
     1801 gggaagcgat gtcgctctgg acgatcaact ctatcaggaa ccgcaagccg ctcccgttgc
     1861 tgtacccatg gggaaattcg ccatgtatcc ggactggcaa cccgatgccg attttatccg
     1921 cctggcggcg ctatggggtg tggcactaag agagccggtg accaccgaag aactggcctc
     1981 attcattgcc tactggcagg cggaaggtaa agtctttcac catgtgcagt ggcaacaaaa
     2041 actggcgcgc agcctgcaaa tcggtcgtgc cagcaacggc ggactgccga aacgagatgt
     2101 gaatacggtc agcgaacctg acagtcaaat tccaccagga ttcagagggt aacgatgaaa
     2161 aacgttggcg acctgatgca acgcctgcaa aaaatgatgc ctgcccatat caaacctgca
     2221 ttcaaaacgg gtgaagaact actggcctgg cagaaagaac aaggagcgat ccgctccgcc
     2281 gctctcgaac gtgaaaatcg ggcgatgaaa atgcagcgca cctttaaccg ctccggtatt
     2341 cggccactgc atcagaactg ctcctttgag aactatcgcg ttgagtgtga agggcagatg
     2401 aatgcgttaa gcaaagcgcg ccagtacgtc gaagagtttg acggcaacat cgccagcttc
     2461 atcttttctg gtaagccagg aaccggcaaa aaccatctgg cggcggcaat ctgcaacgag
     2521 ctgctgctac gcggtaaatc cgtattgatc atcaccgtgg ccgatattat gtcggcgatg
     2581 aaagatacct tcaggaatag cggtaccagt gaagaacaac tgcttaacga tctgagcaac
     2641 gttgatctgc tggtgatcga tgagatcggc gtgcagaccg aatcgaaata cgaaaaagtg
     2701 atcatcaacc agatcgtcga tcgccgctct tcttccaaac gcccaaccgg gatgctgacc
     2761 aacagtaata tggaagagat gaccaagttg ctgggcgaac gcgtgatgga ccgtatgcgc
     2821 ctgggtaaca gtttgtgggt gatcttcaac tgggatagct accgtagccg ggtaacaggt
     2881 aaagagtatt aatccccgtt tctggcggta tactgcgaca caattccacc ttgagtaatg
     2941 atgatgaaaa ctgttaaaca tcttctgtgc tgtgccattg ccgccagcgc attaatttcc
     3001 accggggtgc atgctgcgtc ctggaaagat gcgctctcca gcgccgccag cgaacttggc
     3061 aaccaaaaca gcacgacaca ggaaggcggt tggtcgctcg cgtcattaac taacttgctt
     3121 agcagcggga accaggcctt aagcgcagat aacatgaaca acgccgcagg cattctgcaa
     3181 tactgcgcga agcaaaagct ggcgtcggta accgatgccg aaaacatcaa gaaccaggtg
     3241 ctggaaaagc tgggcctgaa cagtgaagag caaaaagaag acaccaacta tctggatggc
     3301 attcagggtt tgctgaaaac caaagatggt cagcaactca atctggataa catcggaacg
     3361 actccgctgg cagaaaaggt gaaaaccaaa gcctgcgatc tggtgttaaa gcaggggctg
     3421 aacttcattt cctgaaccgc gaaacgccgc gttttagctg ttacccggct gacgcggctc
     3481 tctcctgccc ttttccctgc tcctggacgg ttttccccct gttacacggg aagcgaccgc
     3541 gatcaaaaaa gtctgtttat aaactaattc ctaacaacat cgcatttttt tgacactaga
     3601 attgcagcaa tatggcgacg ccattacatt gtgcttctca aaaagcttaa acgttggcta
     3661 aacggcccgg tttagtgagg atcgcccgtt gtcagaacta ctctctttcg ccctttttct
     3721 cgcctctgtg ctgatttacg catggaaagc gggacgtaac acctggtggt ttgcagccac
     3781 attaacggtg ctggggctat ttgtcgtttt aaatatcacc ctgtttgcca gcgactattt
     3841 tactggcgat ggtattaacg acgcggttct ctatacctta accaacagcc tgaccggtgc
     3901 tggcgtcagc aaatacatcc tgccgggtat cggcattgtg ctggggctga cagcggtgtt
     3961 cggtgcgctg ggctggatcc tgcgccgtcg tcgccatcat ccgcaccatt ttggttacag
     4021 cctgctggcg ctcttactgg cgctgggttc ggtggacgcc agcccggcat ttcgtcagat
     4081 aacggaactg gtgaaatccc agtcacgcga tggcgacccg gactttgcgg cttattataa
     4141 agagccgtcg aaaactatcc ctgacccgaa actcaacctg gtttatatct acggcgaaag
     4201 tctcgagcgg acctattttg ataacgaagc tttcccggat ctcacgcctg aactgggcgc
     4261 gttgaaaaat gaaggcctgg atttcagcca cacgcagcag ctgccaggaa cggattacac
     4321 gattgcgggc atggtggctt ctcagtgcgg cataccgctg tttgccccct ttgaaggcaa
     4381 cgcctccgcc tctgtctcca gcttcttccc gcagaacatc tgtctgggcg atatcctgaa
     4441 aaactcgggt tatcagaact atttcgtgca gggcgcgaat ctgcgttttg ccggtaaaga
     4501 tgtgttcctg aagtcgcatg gcttcgacca cttatacggc tcagaagagc tgaaaagcgt
     4561 ggtggccgac ccgcactatc gcaacgactg gggattctac gacgataccg ttctcgatga
     4621 agcgtggaaa aagtttgaag agctttcccg ctcaggtcag cgattctcac tgtttaccct
     4681 gacagtcgat acccatcacc cggatggttt tatctcgcgt acctgtaacc gcaaaaaata
     4741 tgattttgac ggtaagccga accagtcatt cagcgcggta agttgcagcc aggagaatat
     4801 cgcggcgttt atcaacaaaa tcaaagcgtc accgtggttt aaagataccg tcatcgtcgt
     4861 ctcttctgac catttagcga tgaacaacac ggcgtggaaa tacctcaata agcaggatcg
     4921 caataacctg ttttttgtca ttcgtggcga caagccgcag caagagacgc tggcagtgaa
     4981 gcgtaacacg atggataacg gcgcgacggt gctggacatt ctcggtggcg ataactatct
     5041 cggacttggt cgtagcagtt tatccgggca gtcgatgtcg gaaatcttcc tcaatatcaa
     5101 agagaaaaca ttggcgtgga agccggatat catccgcctg tggaaattcc ctaaagagat
     5161 gaaagagttc accatcgacc agcagaaaaa catgattgcc ttctcgggta gccatttccg
     5221 tttgccgctg ctgttgcggg tttcagacaa acgcgtggaa ccgctgccgg aaagcgaata
     5281 ctcagcaccg ctgcgtttcc agctggccga tttcgctcca cgcgacaatt tcgtctgggt
     5341 tgaccgttgc tataagatgg cacaactctg ggctccggaa ctggcactct ccaccgactg
     5401 gtgtgtctcg caagggcagc ttggcggtca gcaaattgtt cagcatgttg acaaaacaac
     5461 atggcagggc aaaacggcat ttaaagatac ggtcatcgac atggcgcgtt ataaaggcaa
     5521 tgtcgatacg ctgaaaattg ttgataacga tattcgctac aaagccgaca gtttcatctt
     5581 caacgtcgcc ggtgcgccag aagaggtgaa acagtttagt gggatttccc gtccagagtc
     5641 gtggggccgc tggtccaacg cgcagctggg cgatgaagta aaaatcgagt acaagcatcc
     5701 gctgccaaag aaatttgacc tggtgattac cgccaaagca tacggcaata acgccagccg
     5761 tcctattccg gtacgcgtag gcaatgaaga acaaaccctt gtgctgggca atgaagtgac
     5821 caccaccaca ctgcatttcg ataacccaac cgatgccgac acactggtaa ttgtgccgcc
     5881 ggaacctgtc tcaaccaacg aagggaatat cctcggacac tcgccgcgta agctcgggat
     5941 cggcatggtg gaaattaaag tggtggaacg tgaaggataa ttcctgactg atgtgtaggt
     6001 cgcataaggt gcgacctaca caccttctgc ggcctacaaa acatcattga gaaacagaaa
     6061 aaaacagcgc ccaacacttc cccggacgct gcatggcaac tttaataatc tcgccttatc
     6121 agaaagtaat tacaccctta atcaactcac gattgttaat cacatcgcgc tcgtaggttt
     6181 ctgccagcgt ggcgaacgga tagcgatggg ttaacatcat gtcagcagtg atttttcctt
     6241 ccgccatcag tcgaccgact ttagcaaaat cttccggcgt ggcgttgcgg ctacccatca
     6301 tcgtcgtttc ttttttatgg aattccggat cggagaactg caactcacct ttaaacaggc
     6361 cgacaaatac caccgtgccg ccgtgacgaa tcaaattcac ggtgttattc atcgcatgtt
     6421 gattacctgt cgcgtcgatc actttctgcg ccagcgaacc accaaactgc gcccgtagct
     6481 gcgcgtcaaa atcttcggct gacgggtcca gtaaaggtaa ttccagacgc gttgccacat
     6541 gttcacggcg cgccggactg gtatccgcca ccaccacctg tgcgccatcg gctttagcga
     6601 ttgccgccgc gcccagaccg attggccccg ccccgaccac cagcacctgc tcgccgggag
     6661 caatggctgc gcgacgcacc gcatgagcgc taatagcgaa aggttcaatc aatgctgccg
     6721 cctgcgggtc aataccgtct gcgggcaaaa tgttcgccac cggcacgctc aaatactcac
     6781 taaaaccgcc atcctgatgc acgccaatga ctgaaatttt ttcacagcaa ttggtacgcc
     6841 cgcttttaca cgccgggcat tgctgacagg caacataagg gatcacagca acttgctgac
     6901 catttttaag atcagcaata tttttaccca gcccaacaat ctccccacat atttcatggc
     6961 ctaaaacacg tggataacta aaaaatggtt gatttccacc ccaggcatga atatcggtac
     7021 cgcaaatccc gacagactta atttttatta atgcttcatt gtcacccgga ataggtatct
     7081 cgcgttgttt ccagactaat tctttcggct gctggcaaat taaaacattc atcgtagaca
     7141 tgattcggct ctccattttt tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg
     7201 aacggaaaat ctgttttgtg aaaacacacg cataaaaatg ttttaaatcg ggttttaatg
     7261 gactcacaag gaggaaaata tgagtcgttc acaaaattta cgccacaatg tgattaacca
     7321 ggtcattgat gatatggcgc ggggtcatat cccttcccca cttccatcgc aaagtgcgct
     7381 ggcggaaatg tacaacatca gccgcaccac agtgcgtcat attctcagcc acttacgcga
     7441 atgcggcgtc ttgacgcagg ttggaaacga ctatgttatt gcgcgtaaac ctgaccatga
     7501 tgacggtttc gcctgtacta ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc
     7561 ttttttcacg atgataaacc agcgccagtt acgcccaggg gaaacgtttt ctgaactgca
     7621 actggctcgg gccgcgggtg tcagcccggt tgtagtaaga gaatatcttt taaaattcgg
     7681 ccgttataat cttattcaca gcgaaaagcg cggccagtgg agcatgaagc aattcgatca
     7741 atcctacgcc gagcagctgt ttgagctacg cgaaatgctc gaaacacatt cacttcagca
     7801 ctttctcaac ctgcctgatc atgatccccg ctggctgcag gcgaaaacca tgctcgaacg
     7861 tcatcggcta ctgcgtgaca acatcggcaa tagctttcgc atgttctcgc aacttgacag
     7921 agattttcac tccttactgc tttcagctgc cgacaatatc ttttttgatc aatcgcttga
     7981 gatcatctca gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg
     8041 caatatcatt gccgtcgatg agcatatgac cattctcagc gctctgattt gccgcagtga
     8101 ccttgatgcc acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg
     8161 atccattaat gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt
     8221 agcgggtgca cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca
     8281 caatcagtcg actaaaaatg tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc
     8341 cgttaatgtc aaccttgcgt aattcacaat tacgtaaaca attaaaatta taaatacagg
     8401 agtccggcgt ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt
     8461 cgtcggcaga tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac
     8521 gcattcaaac caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc
     8581 gcagttcgct gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg
     8641 aaatcggcgc tttgctctcc gtgttttcac tcgcttacgg gattgcgcaa cttccttgcg
     8701 gcccactatt ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt
     8761 cactgttcca ggcaatgtct ggcatggtgc acaactttac gcagttcgtg ttggtgcgta
     8821 tcggtatggg gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact
     8881 ggttcaacat caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg
     8941 gcgttgccgt aagcccaccg attctggcgg cgatgatgct ggtgatgggc tggcgcggga
     9001 tgtttattac cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc
     9061 gcaaccgcga gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg
     9121 tcaatgcccg ccgagatccg ctcagttttg ccgaatggcg cagcctgttc cgtaaccgta
     9181 caatgtgggg aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg
     9241 cctggcttcc tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga
     9301 tggcggctat ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg
     9361 actggctggt caaaggggga atggctccga ttaaaagccg taagatctgc attattgccg
     9421 ggatgttctg ttctgccgcc tttacgctgg tcgtaccgca agcgacaaca tccatgacag
     9481 cggttctgct gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg
     9541 gcttgatcca cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact
     9601 ttgccagctt catctgcgcc tcttttgcgc cgatcattac tggttttatt gttgatacca
     9661 cccactcatt ccgtctggca ctaatcatct gcggttgcgt caccgcagcg ggggcactgg
     9721 cgtacatctt cctggttcgt cagccgatca acgacccacg taaagattaa tctccttatg
     9781 cccgataaca ttttgcttat cgggcatttt catggcgatt aaaatgtttc ccagttctcc
     9841 tcgctatctg ccacggccat tttacgcggc gcagctggcg tcacggtttt taccacagcc
     9901 gatgtttcac gctgctgttg ctgaatccgg aacactgcca ctgcttcggt caggcgactg
     9961 gcctgctctt ccagcgcggc ggcggcagcg gcagactctt ccaccagcgc ggcgttctgt
    10021 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10081 tcatcagaag cagaagcaat ttcgcccata atgtccgtca cgcgggtcac ggcgctgaca
    10141 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10201 accgagtctt caatcaggct tttaatttca cgagccgcct gggcgctgcg ctgggccaga
    10261 ttacgcactt ctcccgcgac caccgcaaaa ccgcgccctt gctcacccgc acgcgcagcc
    10321 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg caatgccgtc aattacgctg
    10381 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10441 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaacgccag atggctggcc
    10501 tggcgcgcat tttcggcgtt ctgcttcacc gttgcggtca gttactccat gctggctgcc
    10561 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcgtt attgccggtg
    10621 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10681 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    10741 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    10801 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    10861 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    10921 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    10981 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11041 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11101 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11161 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11221 gaaccgctac caatattgtt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11281 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tggattgctg ctggcgaatg
    11341 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaagaa cagaccgcct
    11401 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11461 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11521 caaaaacttt atgaaaaggt gtaacccacc atccatggaa aaaccaggcg ctggttatca
    11581 gatactaaaa agttattcat tatcgcccgg gatggcttct tgtcgggaat tgcccagagc
    11641 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    11701 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    11761 tttggttagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    11821 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    11881 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    11941 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12001 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12061 aaaatcatta ata
//
LOCUS       DAZRUF010000123        11928 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZRUF010000123 DAZRUF010000000
VERSION     DAZRUF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.123"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            147..1224
                     /gene="ygjJ"
                     /locus_tag="ACHXOL_002662"
                     /pseudo
     CDS             147..1224
                     /gene="ygjJ"
                     /locus_tag="ACHXOL_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1241..3592
                     /gene="ygjK"
                     /locus_tag="ACHXOL_002663"
     CDS             1241..3592
                     /gene="ygjK"
                     /locus_tag="ACHXOL_002663"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR6809610.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            3818..5836
                     /gene="fadH"
                     /locus_tag="ACHXOL_002664"
     CDS             3818..5836
                     /gene="fadH"
                     /locus_tag="ACHXOL_002664"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR6809611.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLNVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(5881..6297)
                     /gene="higA"
                     /locus_tag="ACHXOL_002665"
     CDS             complement(5881..6297)
                     /gene="higA"
                     /locus_tag="ACHXOL_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR6809612.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6294..6608)
                     /gene="higB"
                     /locus_tag="ACHXOL_002666"
     CDS             complement(6294..6608)
                     /gene="higB"
                     /locus_tag="ACHXOL_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR6809613.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(6893..8029)
                     /gene="rlmG"
                     /locus_tag="ACHXOL_002667"
     CDS             complement(6893..8029)
                     /gene="rlmG"
                     /locus_tag="ACHXOL_002667"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR6809614.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8114..8617
                     /locus_tag="ACHXOL_002668"
     CDS             8114..8617
                     /locus_tag="ACHXOL_002668"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR6809615.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            8694..9377
                     /locus_tag="ACHXOL_002669"
     CDS             8694..9377
                     /locus_tag="ACHXOL_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR6809616.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9456..10442
                     /locus_tag="ACHXOL_002670"
     CDS             9456..10442
                     /locus_tag="ACHXOL_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR6809617.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            10725..11690
                     /gene="alx"
                     /locus_tag="ACHXOL_002671"
     CDS             10725..11690
                     /gene="alx"
                     /locus_tag="ACHXOL_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR6809618.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2842 a         3070 c         3217 g         2799 t
ORIGIN      
        1 caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt cctggatgag
       61 gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa aggctgtact
      121 ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc agagcattac
      181 ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt ccggcagaac
      241 atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt cgtttttacg
      301 gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa taaacataaa
      361 tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc ctggttcggc
      421 ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc ctgggtgcca
      481 gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg ttatcgtacc
      541 aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga tgacctgcaa
      601 tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg cgacattatg
      661 actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac cgcgactcct
      721 gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga tcgctatgac
      781 gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct tgccgaaggc
      841 atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta tctcacgggc
      901 ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc cgacgggctg
      961 gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc gttaaagggc
     1021 gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc tgacgatgaa
     1081 accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg tttcgccacg
     1141 gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga ccaaatcttc
     1201 ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa aaactatttt
     1261 aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta acgccgacaa
     1321 ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt acgattacga
     1381 cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc atctgttgcc
     1441 agacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg aagagtacat
     1501 caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca agaaagtcga
     1561 ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga cagcaaaaga
     1621 tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac tggaaaccaa
     1681 aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg aaaaactgga
     1741 agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc ctgactatca
     1801 gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag tgcgcgccac
     1861 ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc tgccggtgca
     1921 gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt cgaccacgct
     1981 ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc aaatgcagat
     2041 ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac gctgggaaga
     2101 atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc gcgtcgcggt
     2161 gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg tgaaatttaa
     2221 caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct ggccgtggga
     2281 tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca aagagaatat
     2341 ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc aggatgtggg
     2401 ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg atggcggcaa
     2461 ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg aagtgtacaa
     2521 cgtcacccag gataaagcct ggttggcaga gatgtacccg aaactggtgg cctatcacga
     2581 ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg gcgcgacccg
     2641 cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa aaggcgataa
     2701 agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag gcctgtacga
     2761 cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg atgatgccgc
     2821 cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg gcggcaaacg
     2881 tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt tgctgggcta
     2941 ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata accattatct
     3001 ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc gccagttggc
     3061 acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc agttctacta
     3121 tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac cgattgttga
     3181 gcgcggtaaa gggccggaag gctggtcgcc gctgtttaac ggtgcggcaa cgcaggccaa
     3241 cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct ttgtcccgct
     3301 gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc gtgggcgcgt
     3361 atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt atcgtgatga
     3421 tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg ccgatggccc
     3481 aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa atttctcctg
     3541 gagtgccgct catttgtata tgttgtataa cgattttttc cgtaagcagt aatttgcaac
     3601 ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc ccaggccgga
     3661 taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt tgaatcccat
     3721 cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta gaactcatcc
     3781 gaccacataa caattatttt acatactgga caattttatg agctacccgt cgctgttcgc
     3841 cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct caatgcacac
     3901 cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg ccgaacgcgc
     3961 ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa caggcgttgg
     4021 catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc gcaccattac
     4081 cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata ccgggcgcta
     4141 cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca accgtttcgt
     4201 tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg cccactgcgc
     4261 gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg aagggtattt
     4321 gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg gcggcgatta
     4381 ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac gcgtcggcaa
     4441 cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg gcgggacttt
     4501 tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca ttatcaacac
     4561 cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc cgcgcggcgc
     4621 atttagctgg gtcacgcgca aactgaaagg ccacgtctcg ctgccgctgg taaccaccaa
     4681 ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg ccgatatggt
     4741 atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc aatcggggcg
     4801 agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc agatcttcgt
     4861 tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca aaatgccaat
     4921 ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg ctgggctggc
     4981 gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg cccacagcga
     5041 gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt tttacgaaac
     5101 gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac tcaatcacac
     5161 cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg ggatcgtgcc
     5221 gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc tcaatgtact
     5281 gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg ggattggttt
     5341 tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata tcgccaggtt
     5401 ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa gcccgcaggg
     5461 aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag ccagcaaacc
     5521 gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc tctcgcgggg
     5581 tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc tgcatgtggt
     5641 gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg cagggcaaga
     5701 gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc atttaattgg
     5761 cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg gaacacggct
     5821 ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg gcacgtcgcg
     5881 ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt tagcgtgttc
     5941 cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac tgccaatttc
     6001 cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca cggctatacc
     6061 gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg attcttccca
     6121 cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt tgagcagcag
     6181 atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac ccgccagaaa
     6241 aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg caatcatttt
     6301 ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt cataacttca
     6361 cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa ttcattgccc
     6421 ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga tgggaatact
     6481 gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc cagagccacc
     6541 aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt ttgagttatc
     6601 aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc cgttgacaga
     6661 ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa cgctaaacaa
     6721 gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat tccagaactg
     6781 ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag taacgtgctg
     6841 aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa tcttaccgac
     6901 gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg atggtggtgc
     6961 agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg gcaacaatat
     7021 acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc caggcgacgt
     7081 tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca ttaaagcgga
     7141 aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct aacgcctctg
     7201 gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat tcatcgacaa
     7261 acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg ccattaccgc
     7321 aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata aagaagcgtg
     7381 cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata gtccagtcag
     7441 taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt tcattgaaag
     7501 tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg ccgagcactt
     7561 tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg gcaataatac
     7621 gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc aatgccagtg
     7681 ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg gtgctgtcga
     7741 gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc gtcgccagtt
     7801 cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg cagcttaacg
     7861 caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg tccaactgtt
     7921 gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca tccgtcgccg
     7981 gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc ggccctcctg
     8041 aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct ttttctcttt
     8101 ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc agctactttc
     8161 ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa aacgctatcc
     8221 gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg atctaaaaaa
     8281 tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata agatccacgt
     8341 actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg gcaagctgaa
     8401 agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac cgtttttacg
     8461 catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca aagcgtttta
     8521 ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca cccggctgtg
     8581 gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg cgttatcttt
     8641 tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg accatgcagc
     8701 cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc tgcttactgt
     8761 tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac gccagcaagc
     8821 agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta ttgctggggg
     8881 cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg ttgtaccacg
     8941 tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat tacgatgaag
     9001 cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg atcttctgcg
     9061 actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg tttggggaaa
     9121 accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg ctggcaaaac
     9181 agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag cacggttttt
     9241 atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc aaaatcctcc
     9301 atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt gagcatggct
     9361 gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc gtgctacatt
     9421 gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct gtgattggta
     9481 cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa tacaagttaa
     9541 ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat ttttctgtcg
     9601 agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac gcggtgtata
     9661 ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc cataaaattc
     9721 atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc gccattgcct
     9781 gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc ctgccgaact
     9841 ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc tttttcaact
     9901 attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc aacaccttta
     9961 atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg gcgtcggcgg
    10021 tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg gcaagcggcg
    10081 tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg ttgcagcact
    10141 caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga tcgctggtga
    10201 ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc cagatgcagg
    10261 atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg tttgctaccc
    10321 tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc accgccaaac
    10381 tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc gtaaaactat
    10441 aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg ctgaatggtc
    10501 tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc gtcattacga
    10561 tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag acgtattcct
    10621 tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa gcagcggctg
    10681 acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat actgtcggca
    10741 cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct atcgacctgt
    10801 tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg gcctggtcgc
    10861 tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat ctggtgcaaa
    10921 ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt tatctgattg
    10981 agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc tatttctctg
    11041 ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg attgttctgc
    11101 gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg atcctgtata
    11161 tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat gaagatgaat
    11221 caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc atgaccgaca
    11281 ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc acaccgctga
    11341 tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat agcattccgg
    11401 ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg tttgcgatcc
    11461 tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc tcgatgctca
    11521 aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt gtcgacttct
    11581 accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg atgacgttta
    11641 ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga ttcgctgtat
    11701 attaagccat tcctagagat aactcattcc agacaaaggt gagctattaa ttatgtaatc
    11761 ggcttgtagc tctttacaaa aagaccgcac gggtgttggt ggcgtcaaaa tcgccgaagc
    11821 gttccctgga ggccggggcg acgcgcatgg atgcgcggcg agggcgcatc ttacagggac
    11881 gttcccttgc gcccgacccg gtagccgcaa gggataagct gagggaat
//
LOCUS       DAZRUF010000124        11919 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZRUF010000124 DAZRUF010000000
VERSION     DAZRUF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.124"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHXOL_002672"
     CDS             complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHXOL_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR6809619.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="ACHXOL_002673"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="ACHXOL_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR6809620.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHXOL_002674"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHXOL_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR6809621.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="ACHXOL_002675"
     CDS             complement(3668..3898)
                     /locus_tag="ACHXOL_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR6809622.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4120..4274
                     /locus_tag="ACHXOL_002676"
                     /pseudo
     CDS             4120..4274
                     /locus_tag="ACHXOL_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4320..4736
                     /gene="hicB"
                     /locus_tag="ACHXOL_002677"
     CDS             4320..4736
                     /gene="hicB"
                     /locus_tag="ACHXOL_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR6809623.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            4815..6221
                     /locus_tag="ACHXOL_002678"
                     /pseudo
     CDS             4815..6221
                     /locus_tag="ACHXOL_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHXOL_002679"
     CDS             6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHXOL_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR6809624.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7629..8642
                     /locus_tag="ACHXOL_002680"
     CDS             7629..8642
                     /locus_tag="ACHXOL_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR6809625.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8643..9543
                     /locus_tag="ACHXOL_002681"
                     /pseudo
     CDS             8643..9543
                     /locus_tag="ACHXOL_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9533..10327
                     /locus_tag="ACHXOL_002682"
     CDS             9533..10327
                     /locus_tag="ACHXOL_002682"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR6809626.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10349..11773
                     /gene="patD"
                     /locus_tag="ACHXOL_002683"
     CDS             10349..11773
                     /gene="patD"
                     /locus_tag="ACHXOL_002683"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR6809627.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         2877 a         2897 c         3198 g         2947 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ctaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ctagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag gttattattc
     3961 agttttgcaa attagcgcaa agaaattctg gaatcttcct tcctgatttt gcattgcatt
     4021 ctgccgttgc ggcgatttag tgctattttc gagcaaatta cacatagggg taaacactaa
     4081 tgtttacttt attgatatac tcagcggcag ggaggcgatg tgaaacaaag cgagttcaga
     4141 cgttggctcg aatctcaggg cgtcgatgta gcgaatggcc gcaaccattt gaaactcagg
     4201 tttcatggga ggcgcagtgt catgccgcgt cactcctgcg taaagcaatc ctgaaacaac
     4261 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagagaca gtcgaaatta
     4321 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
     4381 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
     4441 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc ccgttacctt
     4501 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaagtat
     4561 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
     4621 gcaaacctaa acaggagatt actcgcctat ttaacttgca ccatgcgaca aaaatcgacg
     4681 ccgtccagct tgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
     4741 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
     4801 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
     4861 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
     4921 caggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt cagggatata
     4981 ttatcgcacg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
     5041 cgccagtcat tccagtcact tgagatgaag cagtcgatat caacacttat atttttgata
     5101 tgttgcaggc cagtcgcgaa ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
     5161 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
     5221 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     5281 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     5341 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     5401 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     5461 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     5521 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     5581 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     5641 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     5701 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tgtctggtgc
     5761 ctggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     5821 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     5881 ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac
     5941 aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga agttaaaatt catcatagtg
     6001 acagcggtta ttttctctgg ctggagctcc ccgagccgtt agatgccggc gaattaagcc
     6061 tggcggcact gacgcatcat atcagtattg cgccgggtaa aatgttttct accggtgaaa
     6121 actggtcacg tttttttcgt tttaataccg cgtggcagtg gggagaacgt gaagaacagg
     6181 cggtaaaaca attaggcaaa cttattcaag aacggctgta atagcgttta atttaattcc
     6241 tcttagattg ggtaatatga atttcgaata gcagtcatat ttcctaaccc cttgtctata
     6301 ctccagaaga taaccttaca gacggcataa tgcgcggtag ctcacaacct gattaaattt
     6361 tctcagggac gaaggtgtgc ctgcaagccg ccgtctatgg ttaaacaagg agatattttt
     6421 acggcacggc ggctgaacaa ttaattacga caggagtaag accttatgag caagacattt
     6481 gcccgcagca gcctgtgtgc gctcaccatg acaataatga ccgctcacgc cgccgaacca
     6541 cctaccaatt tagataaacc ggaagggcga ctggatatta tcgcctggcc gggatacatc
     6601 gaacgcggac aaactgataa acaatacgac tgggtaacgc aatttgaaaa agagacaggc
     6661 tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa
     6721 gggggttacg atctggttac ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa
     6781 cgcgtgcagc cgattaatac cgccttgatt cccaactgga aaacgctcga tccgcgcgtg
     6841 gttaaaggcg actggtttaa tgttggcggc aaagtttacg gcacacctta ccaatggggg
     6901 ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc cgccggatag ctggcaagtg
     6961 gtttttgttg agcaaaatct gccggacggc aagagcaata aaggccgcgt tcaggcttat
     7021 gatggcccta tctacattgc ggacgctgcg ttgttcgtta aagccactca gccgcagttg
     7081 ggcatcagcg atccgtatca actaaccgaa gaacagtacc aggcggtgct gaaagtgctg
     7141 cgcgctcaac acagtttgat ccatcgctac tggcatgaca ctaccgtgca aatgagcgat
     7201 ttcaaaaacg agggtgtggt tgcttccagt gcctggccct atcaggccaa cgccctgaaa
     7261 gccgaaggcc agcctgttgc taccgttttc ccgaaggagg gtgttaccgg ttgggctgat
     7321 accaccatgc tgcatagcga agcgaaacat ccggtttgcg cctacaaatg gatgaactgg
     7381 tcattaacgc caaaagtgca gggcgatgtg gcggcctggt ttggctcgtt accggtagtg
     7441 ccggaagggt gtaaagccag tccgttatta ggcgaaaaag gttgtgaaac caacggtttt
     7501 aactatttcg acaaaatcgc cttctggaaa acgcctatag cagaaggggg caagtttgtt
     7561 ccctacagtc gctggacgca ggattacatt gccattatgg gcggtcgcta acttcgctgg
     7621 ggtgctttat gacgtacgca gtggagtttg acaacgtctc gcggttgtac ggtgacgtgc
     7681 gcgcagtaga tggcgtcagt attgcgataa aagatggtga gttcttctct atgctggggc
     7741 cgtccggctc cggcaaaacc acctgcctgc gcctgattgc tggcttcgaa cagctttccg
     7801 gcggggctat ctctatcttt ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg
     7861 tgaatactgt ctttcaggac tacgcgctat ttccgcatat gtcgattctt gacaatgtcg
     7921 cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg
     7981 cgctggagaa agtggcgttg gggtttgtac agcaacgtaa accgtcacaa ctttctggtg
     8041 gtcagcgcca gcgggttgct atcgccagag cattggtgaa tgaaccgcgc gtattgctgt
     8101 tggatgaacc gctcggcgca ctggatctca aattgcgtga gcagatgcag ctggaactga
     8161 aaaaactgca acagtctctc ggtatcactt ttatcttcgt tacccacgat cagggcgaag
     8221 cgttatcgat gtccgatcgt gtggcggttt tcaataatgg acgcattgag caggtcgatt
     8281 ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt tgccgggttc gttggtacat
     8341 cgaatgtttt tgatggactg atggcagaga aactttgtgg catgacggga agcttcgccc
     8401 tgcgaccgga acatatccgc ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc
     8461 aggcggtgca atatcagggc gcggcaactc gttttgaact gaaattgaac ggcggtgaaa
     8521 aactgcttgt gagtcaggcc aatatgacag gcgaagaact gcctgccacg ctcacgcccg
     8581 gacaacaggt gatggtttcc tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt
     8641 gaatggcgat gaatgtattg caatcacctt cacgtccagg tctggggctg tttttactgc
     8701 tgcttggccc gctaatgtgg tttggcattg tctatttcgg ctcgctgctg acactgttat
     8761 ggcagggatt ttatactttt gacgatttca ccatgtcggt aacgccggaa ctgacgctgg
     8821 cgaatatccg tgcgctgttt aatccggcga attacgacat cattctccgc acgctgacta
     8881 tggctgtggc agtcactatc gccagcgcca ttctggcttt tccaatggcg tggtatatgg
     8941 cgcgctatac cagcgggaaa atgaaagcgt ttttatattg cggtaatgtt gccgatgtgg
     9001 gcgagctaca ttgttaaagc ctatgcctgg acgttattgc tggcaaaaga tggcgtggct
     9061 cagtggtttt tacaacatct tgggctggaa ccactgctga ctgcgttcct tacattacct
     9121 gcggtgggcg gaaatacgct gtcaacttcc gggctggggc gctttctggt gtttctctat
     9181 atctggttgc cgttcatgat cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca
     9241 ttgttgcagg cgtcggctga tctcggcgca cgtccacgac aaacctttcg ctatgtggtg
     9301 ctgccgctgg caatcccggg tattgccgct ggctctatct ttaccttctc actcacactg
     9361 ggcgatttta tcgtcccgca gctggttggt cctccaggat attttatcgg caatatggtt
     9421 tattcccagc agggggcgat tggcaatatg ccgatggcgg cggcattcac cctggtgccg
     9481 attattctca tcgcactgta cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc
     9541 tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc ggcgttgttt tcctacattt
     9601 tcccatcctg ataatcgccg cctatgcgtt taacactgaa gatgcggcgt ttagttttcc
     9661 accgcagggc ctgacgctgc gctggtttag cgtggcagca cagcgtagtg atattcttga
     9721 tgccgtgaca ctgtcactta aagtggcggc gctggcgaca ttaattgcgc tggtgttagg
     9781 gacgctggca gctgccgcgc tgtggcgacg agactttttc ggcaaaaacg ccatttcgct
     9841 gttactgctg ctgcccattg cgctgccggg cagcgtcact ggtctggcgt tattaaccgc
     9901 ctttaaaacc atcaatctgg agccgggatt tttcaccatc gtggtcggtc atgcgacttt
     9961 ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt cgccgcacct cctggagtct
    10021 ggttgaggcg tcaatggatc ttggggccaa tggctggcaa accttccgct acgtagtgtt
    10081 gccgaatctc agttcggcgt tactggcagg aggaatgctg gcgtttgcct tgtcgttcga
    10141 tgaaatcatc gttacgacct ttacggcagg tcatgaacga acgttactgt tgtggttgct
    10201 caatcagctt gggcgaccgc gtgatgtacc ggtaactaac gtggtggcac tgctggttat
    10261 gttggtaaca accttgccga tcctgggggc ctggtggcta acccgcgaag gcgacaatgg
    10321 ccaataacca ctgatacagg aatatgctat gcaacataag ttactgatta acggagaact
    10381 ggttagcggc gaaggggaaa aacagcctgt ctataatccg gcaacggggg acgttttact
    10441 ggaaattgcc gaggcatccg cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc
    10501 atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc
    10561 tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg gagtcccgta attgtggcaa
    10621 accgctgcat agtgcgttca atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt
    10681 cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt gaatatcttg aaggtcatac
    10741 ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct atcgcaccgt ggaattatcc
    10801 gctgatgatg gccgcgtgga aacttgctcc ggcgctggcg gcagggaact gcgtagtgct
    10861 taaaccatca gaaattaccc cgctgaccgc gttgaagttg gcagagctgg cgaaagatat
    10921 cttcccggca ggcgtgatta acatactgtt tggcagaggc aaaacggtgg gtgatccgct
    10981 gaccggtcat cccaaagtgc ggatggtgtc gctgacgggc tctatcgcca ccggcgagca
    11041 catcatcagc cataccgcgt cgtccattaa gcgtactcat atggaacttg gtggcaaagc
    11101 gccagtgatt gtttttgatg atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt
    11161 tggctattac aatgctggac aggattgtac tgcggcttgt cggatctacg cgcaaaaagg
    11221 catttacgat acgctggtgg aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc
    11281 gccggatgac gagtctacgg agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt
    11341 cagcaaggca gtagaagagg cgaaagcgac agggcacatc aaagtgatca ctggcggtga
    11401 aaagcgcaag ggtaatggct attactatgc gccgacgctg ctggctggcg cattacagga
    11461 cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg agtgttacgc ccttcgacaa
    11521 cgaagaacag gtggtgaact gggcgaatga cagccagtac ggacttgcat cttcggtatg
    11581 gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg
    11641 ggtcaatacc catttcatgc tggtaagtga aatgccgcac ggtgggcaga aactttctgg
    11701 ttacggcaag gatatgtcac tttatgggct ggaggattac accgtcgtcc gccacgtcat
    11761 ggttaaacat taagttagat gttagtggct gttataggac ggccactatc gttttaggtt
    11821 ctgagtttaa tggcggttaa gtattagaat gattacatta ataactagcg taaaaattag
    11881 gctaaatgtt tgatggcgat ttttcatcct gtcacagtt
//
LOCUS       DAZRUF010000125        11914 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZRUF010000125 DAZRUF010000000
VERSION     DAZRUF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11914
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.125"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            144..710
                     /locus_tag="ACHXOL_002684"
     CDS             144..710
                     /locus_tag="ACHXOL_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6809628.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            866..2898
                     /locus_tag="ACHXOL_002685"
                     /pseudo
     CDS             866..2898
                     /locus_tag="ACHXOL_002685"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2875..3219
                     /locus_tag="ACHXOL_002686"
     CDS             2875..3219
                     /locus_tag="ACHXOL_002686"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR6809629.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            3234..3965
                     /locus_tag="ACHXOL_002687"
     CDS             3234..3965
                     /locus_tag="ACHXOL_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR6809630.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3962..5023
                     /locus_tag="ACHXOL_002688"
     CDS             3962..5023
                     /locus_tag="ACHXOL_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6809631.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVTPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5175..6221
                     /locus_tag="ACHXOL_002689"
     CDS             5175..6221
                     /locus_tag="ACHXOL_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR6809632.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6218..7009)
                     /gene="nei"
                     /locus_tag="ACHXOL_002690"
     CDS             complement(6218..7009)
                     /gene="nei"
                     /locus_tag="ACHXOL_002690"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR6809633.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7045..7779)
                     /gene="pxpA"
                     /locus_tag="ACHXOL_002691"
     CDS             complement(7045..7779)
                     /gene="pxpA"
                     /locus_tag="ACHXOL_002691"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR6809634.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7769..8701)
                     /gene="pxpC"
                     /locus_tag="ACHXOL_002692"
     CDS             complement(7769..8701)
                     /gene="pxpC"
                     /locus_tag="ACHXOL_002692"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR6809635.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8695..9351)
                     /gene="pxpB"
                     /locus_tag="ACHXOL_002693"
     CDS             complement(8695..9351)
                     /gene="pxpB"
                     /locus_tag="ACHXOL_002693"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR6809636.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9374..10117)
                     /gene="ybgI"
                     /locus_tag="ACHXOL_002694"
     CDS             complement(9374..10117)
                     /gene="ybgI"
                     /locus_tag="ACHXOL_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR6809637.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10388..11869
                     /gene="dtpD"
                     /locus_tag="ACHXOL_002695"
     CDS             10388..11869
                     /gene="dtpD"
                     /locus_tag="ACHXOL_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR6809638.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2870 a         3172 c         2900 g         2972 t
ORIGIN      
        1 acatttaatt cataccatca attaattact tttacattta ataaaattaa taatattttt
       61 aagaaaaact aaaaacattc gacaatcaaa taagatattc ttgcaccgat atactttttt
      121 cttttctgtt aaaggactca gttatgttta aaggacaaaa aacattggcc gcactggccg
      181 tatctctgct gttcacagca cctgtttatg cggctgatga aggatccggt gaaattcact
      241 ttaaaggtga agttattgaa gcaccgtgtg aaatacatca ggatgatatt gataaagagg
      301 ttgaactcgg tcaggtgacc accagccaca ttaatcagtc acatcacagc gatgccgttg
      361 ctgtcgacct gcgcttagtc aactgtgatc tggaaaactc cagcaacggt tccggtggca
      421 agatttctaa agttgcagtc acctttgata gctcagcgaa aaccaccggc gcagatccga
      481 ttctcaacaa caccagcacg ggtgaagcca ccggcgtcgg cgtacgttta atgaataaag
      541 atcaaagcaa tatcgtttta ggcaccgcta ctccagatat cgacctggct ccgacctcca
      601 gcgaacaaac gctgaatttc tttgcctgga tggaacaaat tgatcaggct acacctgtaa
      661 cgccaggcgc agttacggcg aatgcaacct acgtgctgga ttataaataa gtattacttt
      721 ggcggcgcta tcgccgcctt tcataaaata tttatcaggt atggacaccg tgaatattta
      781 tcgactctct tttgtatcct gcctggtcgt ggcgatgcct tgcgcattgg cggtcgaatt
      841 caaccttaat gttctcgata aatcgatgcg cgaccgcatt gatatttcat tattaaagga
      901 aaaaggagtc attgctcccg gtgagtattt tgttagcgtt gcggtaaata ataaccaaat
      961 cagtaacggg caaaagatta actggcacaa aaatgacgat aaaaccattc cgtgcatcaa
     1021 tgatttactg gtcgataaat ttggcttaaa acctgaagtc cgtcagtcgt taccattgat
     1081 aaatcagtgc gtcgatttta gctcccgacc tgaaatgctc ttcaatttcg atcaagccaa
     1141 tcagcaactg aatattagta ttccgcaagc ctggctggcg tggcactcag aaaactggac
     1201 cccaccctcc acatggaaag aaggtgtcgc cggtatcctg atggattaca acttgtttgc
     1261 cagcagctac cgcccacagg acggcagcag cagcactaac ctgaacgcct acggtaccac
     1321 cggaattaac gccggggcat ggcgcttacg tagtgattat cagttgaatc agactgatag
     1381 cgatgataac catgaacagt caggcggaat atcgcgcacc tatctttttc gtccattacc
     1441 gcaattaggc tctaaattaa ccctcggcga aacggatttt agttccaata ttttcgacgg
     1501 tttttcttat accggcgcgg cactggcaag tgacgagcga atgttgccat gggaactacg
     1561 cggctacgcc ccacaaatta gcggtattgc acagaccaat gccacggtga cgatcagtca
     1621 atcaggccgc gtcatttacc agaaaaaagt cccaccaggc ccatttatca ttgacgacct
     1681 taatcagtct gttcagggca cactggatgt caaagtgacg gaagaagatg gtcgggtgaa
     1741 caatttccag gtttcggcag catcgacgcc cttcctgact cgtcagggac aggttcgcta
     1801 taaactggcc gcgggtcagc cacggccctc catgtcacat caaactgaaa atgaaacctt
     1861 ttttagcaat gaagtttcct gggggatgct gtcaaacacc tcgctgtacg gcggcctgct
     1921 gctttctggt gatgactacc attctgccgc aatgggtatt gggcaaaata tgctgtggct
     1981 tggtgcgctg tcgtttgatg tcacgtgggc cagtagccat tttgatactc agcaggacga
     2041 gcggggctta agctaccgtt ttaattacag caaacaagtg gatgccacta acagcacgat
     2101 ttccctcgcc gcttatcgtt tctccgatcg tcattttcac agctacgcca actatctgga
     2161 tcacaaatac aacgacagcg atgcgcagga cgaaaaacag acgatcagct tattgtgggc
     2221 caaccgatta ccccactaaa cctcaatctt tacgccaacc tgctacatca aacctggtgg
     2281 aatgcagacg cctccacgac cgccaacatc acggcaggtt ttaatgttga tattggtgac
     2341 tggagagata tctctatttc gacgtcattc aatacaaccc attacgaaga taaagatcac
     2401 gacaaccaga tttacctgtc gatttcgctc cccttcggta acggtggtcg ggttggctat
     2461 gacatgcaaa acagtagcca cagcaccaca caccgcatgt cgtggaacga tacgctggat
     2521 gaacgtaata gctggggcat gtctgccgga ctgcaatccg accgtcctga caatggagcc
     2581 caggtgagcg gtaactatca gcacctgagt tcagcgggtg agtgggatat ttctggtacc
     2641 tatgccgcca atgattacag ttccgtcagc agcagctgga gcggttcttt caccgcaacc
     2701 caatatggtg cagcgtttca tcgccgcagc tccaccaatg aaccacgcct gatggtcagc
     2761 accgatggcg tggcagatat tccggttcag ggcaatctcg actacaccaa ccattttggc
     2821 attgcggtgg tgccgttgat ttccagttac cagccttcca ccgtggcggt gaacatgaat
     2881 gacttacccg acggcgtaac agttgcagaa aacgttatca aagaaacgtg gattgaaggc
     2941 gcgataggtt acaaatcact ggcttcccgt tccggtaaag acgttaacgt catcattcgc
     3001 aacgccagcg gtcagtttcc tcccctcgga gcggatatcc gccaggatga cagcggcatt
     3061 agcgtgggga tggttggcga ggaaggacat gcctggttaa gcggagtcgc tgaaaatcaa
     3121 aagtttaccg tggtctgggg tgatagccag cattgctcgc tccatcttcc tgaacatatg
     3181 gaagacaccg caaatcgcct gattttacct tgtcattaat taaggaaatg aaaatgacat
     3241 ttatgaaagg actacccctt ttgttgttgg ttgccagtct gtgcagccac gctgcactac
     3301 aacccgatcg cactcgtatt gtgtttaacg ctaatgataa agctaccagt ctgcgggtgg
     3361 ataatcgtag cgataaactc ccctacctcg cttattcctg gctcgaaaat gaaaaagggg
     3421 agaaaagcga tgatcttctg gtcgccctgc cgccgataca acgtctggaa ccgaaagcaa
     3481 caacgcaggt acggatagta aagcaagcct cgaccacaaa attaccgggc gatcgcgaaa
     3541 cgctattttt ctacaatatg cgtgaaattc ctcccgcccc ggaaaaaaac agcgaccatg
     3601 ccgttctgca agtcgccatt cagagtcgta tcaaggtgtt ctggcgaccg gctgcattac
     3661 gcaagaaagc aggagaaaag gtcgaactgc agttacaggt cagccagcag ggcaaccaac
     3721 tgacactgaa aaatcctacc gcgtattatc tgaccattgc ctacctggga cgaaatgaaa
     3781 aaggcgttct ccccggcttc aaaaccgtga tggttgcgcc ttttagtacg gtcaacacaa
     3841 acaccggaaa ttatagcggc agccagtttt atctcggtta tatggatgat tacggcgcgt
     3901 tacgcatgac gacgctcaac tgcagcggac aatgccattt acaagccgtg gaggcgaaga
     3961 aatgagtgct ggcaagggag tgttgctcgt catctgtctg ttatttctgc cgttgaagtc
     4021 tgcgcttgcc ctgaactgct actttggttc atccggtggc tcggtagaaa aatcagaagc
     4081 gattcaacct tttgctgtac caggcaatgc caaactcggc gataagattt gggaatctga
     4141 cgatattaaa attcccgtct attgtgacaa caataccaac ggcaattttg aaagtgagca
     4201 tgtttatgcc tgggtaaccc cctacccggg ggtgcaggat cgctattatc aactcggcgt
     4261 aacttacaat ggcgttgatt atgacgccaa ccagggaaaa agccgcatcg acactaacca
     4321 gtgtattgac agtaagaaca ttgacattta tacccctgag cagattatcg cgatgggatg
     4381 gcagaacaaa atttgctcgg gtgatcccgc taatattcac atgtcgcgta cctttctcgc
     4441 tcgcatgcgg ttatacgtca aaatacgaga aatgccgccg catgattatc aaagtacgct
     4501 tagcgactat atcgtcgtgc aatttgacgg tgccggtagc gttaacgaag accctactgc
     4561 ccaaaacctg aaatatcata ttactggtct ggaaaacatt cgcgtgctgg attgcagcgt
     4621 caatttttcc atttcaccgg aaacacaggt gattgatttt ggtaaattta atttgctgga
     4681 tattcgtcgc cacacaatgt cgaaaacatt cagtattaag acgacaaaat cacaaaatga
     4741 ccaatgcacc gacggattta aagtcagttc ctccttttat accgaagaaa cgttggttga
     4801 agaagataaa gcattgttga ttggtaatgg tctgaagcta cgtttgctgg atgaaaacgc
     4861 ctcgccttat accttcaata aatatgctga atacgccgat ttcaccagcg acatgttggt
     4921 ctacgaaaaa acctatacag ctgaactttc gtccattgca ggtaccccca tcgaggctgg
     4981 gcccttcgat acagtggtgc tttttaaaat taactataac tgatttgcca tgatgccctt
     5041 tgcagccgga aagggcatct ctttcagaaa attgctgatt ttcccctttt ccttgattgc
     5101 tgttattttt tcctggcccg gctttcttta tgctgtatcg atacaccaaa gagaaaataa
     5161 tgagggagcg aaggatgcca gttttgcagt ggggaatgtt atgtgtgcta tcacttctcc
     5221 tttctattgg tttcctcgcg gtacatctcc cggcggcgct attacttggg ccgatgatcg
     5281 ccgggatcat cttcagtatg cgcggaatta ctctgcaact tccccgctcc gcttttctcg
     5341 ccgcgcaggc cattcttggc tgcatgattg cgcaaaacct cactggttcg attctcacca
     5401 cgctggccgc caactggccg atcgtgcttg ctattttact ggtgacgctg ctttccagcg
     5461 ccatcgtggg ttggttattg gtgcgctata gctcactgcc tggaaatacc ggtgcctggg
     5521 gttcttctcc aggcggcgca gcggcaatgg tcgctatggc gcaagattac ggcgcagata
     5581 ttcgcctggt tgcatttatg caatatctgc gagtgctgtt cgtcgcgggg gccgctgttc
     5641 tggtcacccg catgatgctg ggtgataacg ctgaagcggt taaccagcaa atcgtctggt
     5701 tcccgccagt gagcattaat ctcctgctta cgattttgct cgcagtcgtt gccggtacgg
     5761 cgggatgcat gctgcgtctt ccttccggca cgatgctcat cccgatgctg gcgggcgcag
     5821 tgctccagtc tggtcagctc atcaccatcg aactaccgga atggctgctg gcgatggcgt
     5881 atatggcaat tggctggcgg attggtcttg gtttcgataa acaaatctta ctgcgggcac
     5941 tacgcccgct accgcaaatc ctgctgtcga tttttgctct gctggctatt tgtgcgggta
     6001 tggcgtgggg gctgacccgg tttatgcaca ttgattttat gactgcctac ctcgccacca
     6061 gtcccggcgg gcttgatacc gtggcggtca tcgccgcagg gagcaatgcc gatatggcgc
     6121 ttattatggc gatgcaaacc ctgcgcctgt tcagtatttt gctgacgggg ccagccattg
     6181 cgcggtttat ttcaacctat gcgccgaagc ggtcggccta gtgctggcag ccagggcacc
     6241 agtaaaacgg gcgagatgac agcgtggttt tctcaatgat gctgccacag cgttcgcacg
     6301 gttcgccatc acgatgaaaa accttaaagc gaaacagcgc cccatgatgc ttattctcat
     6361 ccacctgccc cctcgtagcg taggaaaatc gaggaatatc cagtaacgcg tgtgcgagtg
     6421 catccagttg cgccgcattg agatctttcg ctttatgatt tccagtcaac ccaacctgcc
     6481 agaggatctc cacccgcaaa taattgccaa gcccagccag aaacgcctga tcgagcagta
     6541 atcctgcaaa ctgacggtta cgaaagcgcg gtgacaataa ccgttccttt accacctccg
     6601 gcgtcagatt cggatccagc acatcgggac caacgcgttg taaaaacgga tgcgtggtca
     6661 gttgttccgg cctcaacatc tcaatatccg aggcgctata aagcagaatg gttttgtcag
     6721 ccgtttgcag ttttacccgc aatactcgcg tggtctgcgg ctcttcgccg gtatcaacca
     6781 cgcgccagac gccgtaaagc tgattatggc tgtagagcgt taagtcgttg gaaaaatgag
     6841 ttaacaacgc cttaccacgc gtttccacat gggtaacgtg ttgaccgata agttgtgatt
     6901 gataagtttt taactgcggg aaggcaaacc agacatcagt tagtggtttg cctttgatcg
     6961 ccgcctccag gttatccgct gcacggcgga tctccgggcc ttcaggcatg gtgatatcct
     7021 tttttcattc tttattaatc ggggttatgc tgcgacaaca atctcctttt cggcaaatgt
     7081 agagcgtagt cggcgggcga acgccagtgc gtgctcgccg tcgccatgca ggcagactgt
     7141 ttgcgccgtg accgttgccc attcgccggt gatacttttg actctgccgt gttgcaccat
     7201 ctctagcgtt tgcgccaacg cctgttcttc gttttcaatc aacgcgcctc gctgacttcg
     7261 cggcaccagc gagccgtcag cctgataacc gcgatcggca aacacttcct cgcgcgttgt
     7321 caggccatat tgcttgcctg cacgaatcag ctcgcttcct gccagcccaa cgagaatcaa
     7381 tgccggatcg caagcgtata ccgctctggc gatggcgtct gccagttgtg cttctttcgc
     7441 cgcctggttg tacaacatgc cgtgcggttt gacatgacgc attacgccgc cttgcgcacg
     7501 ggcaatcgtt gccagcgcgc caatttgata cagcgtctgg gcgtaaacgg tttctggcgg
     7561 cagctgcatg gcgctgcgac caaaattttc cctgtcggga aaactcgggt gagcgccaat
     7621 cgcgacacca ttttttattg cttcacgcac gcaagcctgc atgatttgcg catcgcctgc
     7681 atgaaatcca caggcaatat tggcagagga aaccagcgtt aatagctctg cgtcgctggc
     7741 gcagccttcg cccagatcgg cgttcaggtc aattttcatt gtgcagccgc cacactaatt
     7801 gttcgaaata acgttgctga tcttgccgcg ctttcagtgc ctcttccagt gaacactgga
     7861 caaaatgaat cggctgaccg agcggaattt gcgccagatg gtacatatca gcctcaatga
     7921 tacaggcaat acgcgggtaa ccatcggtgg tctgtgcgtc gttcatcaac acaatcggct
     7981 gcccgttatg tggcacctgc accacgcccg gtaacaaacc gtgagataac agttcgcgat
     8041 cggtggtgcg ttttaaaatt tgcccctgta agcgatagcc catgcggtta ctttgcgagc
     8101 taagctgcca gggcgaacgc cagaatgcat cctgcgaggc gcgatcgaac tcatgatatt
     8161 ccggccccgg caaggcgcga atgcggttgc cccacagcag ctgtttaacg ccctgcgctt
     8221 ccataaaatc acgcttcgat ttgccaatcg ggagtcggtc accatccttc agtaaacggc
     8281 cttccagccc gccaatcccc actttgagat cggtgctgca tgaccccatt accggcggaa
     8341 catcaatacc acccgcgacc gccagataac tgcgcatccc gtgctgcggg cgtttaagcg
     8401 ttaaacgctg gcctgctttc atcggcaatc gccagccggt ccagacggca ttatcatcca
     8461 gccgcgcttc gcaaccggca cccgtcagag caaaccaccc atcagtttcg aactcaaccg
     8521 ttaactgacc gagcgtgatc tccagcgcgg gggcattggc gtcattaccc accagcaggt
     8581 tagcaatgcg taacgcaggc atatccagtg cgccgcagtg gctgattccc gactggcgaa
     8641 aaccgtgacg accgccatcc tgcacagtgg tatacatgcc cgcacgaata atcttcagca
     8701 tactccctcc ttctgcggta caaagcgcac gctgtctccc ggacgtaata agatgggttc
     8761 gtcacgcgcc ggatcaaaca ggctgagtga ggtatgacca atcaactgcc agccacccgg
     8821 cgttgccagc ggataaacac cagtctgcgg cccgccgatc ccgacagaac ctgccggaac
     8881 gagcaagcgc ggttcagcgc gccgtggcgt gtgtagttgt tccggcaaac tcccgagata
     8941 cgggaagccc ggttgaaaac ctaaaaacca gaccacgtat tccacggagg agtgcaattc
     9001 aacaacctgt ttttcgctaa acccgcaatg cgccgcgacc accgccaaat ccggtccgcc
     9061 tgcaccaccg taaaccaccg gaatttcaat aaagcgagac tcaggttcca gggcctcgct
     9121 ctcctcccac cagcgttgca aacgctctat ggcatccagc gccagcgact caggattacg
     9181 caaaatcacc gtgatattgt tcatgccggg aatggcttca accacattcg gcatatccac
     9241 cagacgctgc gccagtcgcc agatccgttt ctggctagcc agcgtcaccg gcggttccag
     9301 ttccagcact accgccgttt cacctatcag ataacaacgc gctcgttgca ctttcgatcc
     9361 ctctgattat tcgttatgca ggattaggaa tatcaataaa ggtcacatca agatcggtat
     9421 tttcattcag ccactcgctc aatgcgcgaa taccaccacg ttcagtggca tggtgacctg
     9481 cagcataaaa atgcaacccc tgctcgcggg ctgaatgaat ggtctgttca gaaacttcgc
     9541 cagtaataaa agcatccacg ccaaaacgcg cggcgctatc gataaaactt tgcccgccgc
     9601 ccgtgcacca ggcgacgcgc tggaccacct caggtccggt atcgccacac cataacggct
     9661 tacgtcccag acgcgcttca atccaggagg ctagttccag tcccggcaca ggcatggtca
     9721 gctctcccca cggcaccaac ggctcaattt cgcccatgac cgtgatcccc agtaacgccg
     9781 ccagctgtgc gttattgccc agctcaggat gtacgtcaag cggcaaatgc cagccataca
     9841 ggttgatatc attcgccagc aacgttttta aacggttacg cttcattccg cgaatgaccg
     9901 gagactctcc tttccagaag tagccatgat gcacaatgac cgcatccgcg cccagacgca
     9961 ctgcctcatc gagcaaagcc tggctggcgg tgacaccggt aacaattttt tgcaccgtct
    10021 ctttgccttc tacctgcaaa ccattcggcg cgtaatcact aatcgccgcg ctgttcagtt
    10081 tttcgttaat cagttgttcc agttcggtgt ttttcatctt cgcgccttcc gtattctgat
    10141 agctacaccg ttaacatagc gttacctcgc ctgttcgtcg atagccaatc ttttgacata
    10201 ttaagaaaca aacccgacgc caaaagacgc aaattgttta tatcatcacg cttcagctaa
    10261 tgtatattta tgcaatcaga cagcatattg cataaataat cagatctgta tcaaagtttt
    10321 aactttaagg aaacacaagc acgccgcgca actattacgt gacgttgcag tcaattgtgg
    10381 aattattatg aataaacacg catcacaacc gcgcactatt tactatgtcg ttgcgctgca
    10441 aatctgggaa tattttagct tttacggcat gcgtgccctg ctgattctct atctcaccaa
    10501 tcaactaaaa tacaacgata ctcacgccta cgagttattt agcgcctact gttcgctggt
    10561 gtatgtcacg ccaatcctcg gtggcttttt ggcggataaa gttctcggca atcgcatggc
    10621 ggtgatgctg ggggcgttgt tgatggcgat cggtcatgtg gtgctgggtg ccagtgagat
    10681 ccatccgtca ttcctctatc tgtccctggc gattatcgtt tgtggctatg gtctgtttaa
    10741 atccaatgtc agttgcctgc tcggcgagct gtatgagcca accgatccgc gtcgtgatgg
    10801 cggtttctcg ctgatgtatg cagcgggtaa cgtggggtct attatcgcac ctatcgcctg
    10861 tggttacgcc caggaagagt acagctgggc gatgggcttt ggcctggcgg cggtgggtat
    10921 gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat ttcacacata cccgcggcgt
    10981 taataaaaaa gtactgcgtg caacaaactt tctcctgcct aactggggat ggctgctggt
    11041 tctgctggtg gcaacgcctg cgctgattac cgtactgttc tggaaagagt ggtcggtata
    11101 cgccttaatt gtcgcgacta ttattggcct gggtgtactg gcaaaaattt atcgcaaagc
    11161 agaaaaccag aaacagcgta aggagctggg gctgattgtg acgctcacct tcttcagtat
    11221 gttgttctgg gccttcgcac aacagggcgg cagctcaatt agcctttata tcgaccgctt
    11281 cgttaaccgc gatatgtttg gttataccgt tccgactgct atgttccagt cgattaatgc
    11341 cttcgcagtt atgctatgcg gtgtgttcct ggcgtgggtg gttaaagaaa gcgtcgcagg
    11401 taatcgtacc gtgcgcatct ggggaaaatt tgctcttggc cttggcctga tgagcgccgg
    11461 attctgcatt ctgaccttaa gcgcccgctg gtccgcaatg tatggtcact cttctctgcc
    11521 actgatggtg ttaggcctcg cggtgatggg ctttgcggaa ctgtttatcg acccggttgc
    11581 catgtcgcaa attacgcgca ttgaaattcc gggcgtgacc ggcgtattaa ccggcatcta
    11641 catgctgctt tctggcgcga tcgcgaacta tctggcgggc gtgattgccg atcagacatc
    11701 gcaggcttcg tttgatgctt ccggggcgat caactactcc atcaatgcat atattgaagt
    11761 atttgatcaa attacctggg gcgcactggc gtgtgtagga ctggtactga tgatttggct
    11821 gtatcaggcg ctgaaattca gaaaccgcgc gctggcgctg gagtcataat aggccagcac
    11881 cgatgcctga tgcgacgctt gacgcgtctt atca
//
LOCUS       DAZRUF010000126        11811 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZRUF010000126 DAZRUF010000000
VERSION     DAZRUF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.126"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..489
                     /locus_tag="ACHXOL_002696"
     CDS             <1..489
                     /locus_tag="ACHXOL_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR6809639.1"
                     /translation="VNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGH
                     HRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRR
                     RNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNAR
                     LS"
     gene            541..1476
                     /gene="ttcA"
                     /locus_tag="ACHXOL_002697"
     CDS             541..1476
                     /gene="ttcA"
                     /locus_tag="ACHXOL_002697"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR6809640.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1605..2978)
                     /gene="dbpA"
                     /locus_tag="ACHXOL_002698"
     CDS             complement(1605..2978)
                     /gene="dbpA"
                     /locus_tag="ACHXOL_002698"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR6809641.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3008..3181)
                     /gene="ynaL"
                     /locus_tag="ACHXOL_002699"
     CDS             complement(3008..3181)
                     /gene="ynaL"
                     /locus_tag="ACHXOL_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR6809642.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3456..4439)
                     /gene="zntB"
                     /locus_tag="ACHXOL_002700"
     CDS             complement(3456..4439)
                     /gene="zntB"
                     /locus_tag="ACHXOL_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR6809643.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4453..4500)
                     /gene="ydaN"
                     /locus_tag="ACHXOL_002701"
     CDS             complement(4453..4500)
                     /gene="ydaN"
                     /locus_tag="ACHXOL_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR6809644.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4694..5926
                     /gene="dgcM"
                     /locus_tag="ACHXOL_002702"
     CDS             4694..5926
                     /gene="dgcM"
                     /locus_tag="ACHXOL_002702"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR6809645.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5947..6510)
                     /gene="smrA"
                     /locus_tag="ACHXOL_002703"
     CDS             complement(5947..6510)
                     /gene="smrA"
                     /locus_tag="ACHXOL_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR6809646.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6840..7748)
                     /locus_tag="ACHXOL_002704"
     CDS             complement(6840..7748)
                     /locus_tag="ACHXOL_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR6809647.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7924..9234
                     /gene="abgA"
                     /locus_tag="ACHXOL_002705"
     CDS             7924..9234
                     /gene="abgA"
                     /locus_tag="ACHXOL_002705"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR6809648.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9234..10679
                     /gene="abgB"
                     /locus_tag="ACHXOL_002706"
     CDS             9234..10679
                     /gene="abgB"
                     /locus_tag="ACHXOL_002706"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR6809649.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10716..>11811
                     /locus_tag="ACHXOL_002707"
     CDS             10716..>11811
                     /locus_tag="ACHXOL_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR6809650.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNM"
BASE COUNT         3005 a         3001 c         2930 g         2875 t
ORIGIN      
        1 gtgaatgtca gaagaaacct gacgatactt gatatgttcg gtcccccaaa aacaaatgcc
       61 gggatccgga cggtaacatt actgcagccg gctcttgaag cactgaagga gcaatacaaa
      121 ctgaccgggc atcatcgcaa aagcgaaatc actttttatc atcgggagta cggcagaact
      181 gaaaagcaaa aactgcattt tgttttcatg cccagggtgt gtaacgaaaa acagaaacct
      241 tattactcgg taagcagttt gggtgcaaga tggaatgcag cagtaaaacg tgctggtatt
      301 cgccgccgta atccgtacca tacgcgacat acttttgcct gctggctgtt gacggcagga
      361 gcgaacccgg catttatagc cagccagatg gggcatgaaa ctgcgcaaat ggtgtatgaa
      421 atttacggta tgtggattga tgacatgaac gacgaacaga tagccatgtt gaatgcgcgg
      481 ttatcgtagt tgcaaagttt gcccccaatt tgccccattt agtaccagag aactgaaata
      541 atgcaagaaa atcaacaaat tacaaagaaa gaacaataca acctgaacaa attacaaaaa
      601 cgtctgcgtc gtaacgtggg cgaagccatt gctgacttca atatgattga agaaggcgat
      661 cgcatcatgg tttgcctctc cgggggtaaa gacagctata ccatgctgga gattctgcgc
      721 aatttgcagc aaagcgcgcc aatcaatttt tcgctggtgg ctgttaacct cgatcaaaag
      781 caaccgggct tcccggaaca cgttctgccc gagtatcttg aaaagctggg cgttgagtac
      841 aagattgtcg aagagaacac ttacggtatc gtgaaagaga agattccaga gggcaaaacc
      901 acttgctcac tgtgttctcg ccttcgtcgc ggtatccttt atcgtaccgc aacggaactg
      961 ggggcgacga agatcgcgct gggtcaccat cgtgacgata tcctgcagac gctgttctta
     1021 aatatgttct acggcggcaa gatgaaaggt atgcctccga aactgatgag cgatgatggc
     1081 aaacatatcg ttattcgtcc gctggcctac tgccgcgaga aagatattca gcgatttgcc
     1141 gatgcaaaag cgttcccgat tattccgtgc aacctgtgcg gttcacagcc taacctgcaa
     1201 cgtcaggtga ttgctgacat gttgcgtgac tgggataaac gttatccagg gcgtatcgag
     1261 acgatgttca gcgcgatgca gaatgtggtg ccgtcgcatc tgtgcgatac caacctgttc
     1321 gatttcaaag gcatcaccca cggttctgaa gtggttaacg ggggtgatct ggcgtttgat
     1381 cgcgaagaga tcccactaca accggcgggc tggcagccag aagaagatga aaatcagttg
     1441 gatgagttac ggctgaatgt ggttgaagtg aaataaccag gatggcgccc gatgcgcaag
     1501 cgtatcgggc tactcttatg gaggccggat aagacgcggc cagcgtcgca tccggcaatc
     1561 ccgaataaga tgtttactct tgcacccggc aattcaacat ttcattattt taataaccgc
     1621 acccggctcg tttttccttt aatcttcccg ccctgtaact gtttccatgc tttatgagca
     1681 acagcctgac ggaccgcgac atagacatgc gccggatgca cggcgatttt gccaatatct
     1741 gcgccatcaa gcccgatatc tcctgtcagt gcacctaata catcacccgg gcgcattttg
     1801 gcttttttcc caccatcgat acacaacgtt gccatttctg cttccagcgg cacaatggaa
     1861 ctattagctg gcggcgtttg ccagttaagt tttatctgca acatgtcaga aatgatattg
     1921 gcccgctgtg cttcttccgg agcacagaaa ctgatcgcca gaccgctatt tcctgcacga
     1981 gctgtacggc cgatgcgatg tacatgaact tcagggtccc acgccagctc aaagttcacc
     2041 accagctcaa gcgatttaat atccagacca cgcgcagcaa catcagtcgc aaccagtaca
     2101 cgggcgctac cgttagcaaa acgtaccagg gtctgatcgc gatcgcgttg ctccaaatcg
     2161 ccgtgtaatg acaatgcact ttgccctact tcattcagcg cgtcgcagac agcctggcaa
     2221 tcttttttgg tattgcaaaa caccacgcaa gaggatggct gatgcaagct taataaccgt
     2281 tgcaacagag gaattttgcc tttgctggat gtctcataaa attgttgttc aatgggtggc
     2341 aaagcatctg ttgagtcaat ttcaatcgcc aaaggatcgc gttgcactcg tccgctgatt
     2401 acggcaatgg cttccggcca ggttgccgaa aacagaagcg tctgtcgaga tgcaggcgca
     2461 aaacggatga catcatcaat ggcatcgcta aatcccatat ccagcatgcg gtcggcctca
     2521 tccatcacca aagtattcag cgcatccagt gataccgtgc ctttttgcaa gtgatccagc
     2581 aaacgacccg gcgttgccac gataatatgc ggcgcatgtt gcaacgaatc acgctgcata
     2641 ccgaacggtt gaccaccgca caacgtcaaa attttggtat ttggcagaaa acgcgccagc
     2701 cgacgcaatt cacctgccac ctgatccgcc agttcacgcg taggacacag cactaaagcc
     2761 tgggtttgaa atagcgacgc atcaatttgc tgtaacaagc cgaggccaaa agccgccgtt
     2821 ttgccgctgc cggttttcgc ctgcacgcga acatcttttc cggcaaggat cgccggaagc
     2881 gcggcggcct gcaccggcgt catagttaaa taacccaact cattaaggtt cgtgagttgg
     2941 gcgggaggca aaacattcag ggtagaaaaa gcggtcacaa tctattctcg tggtcatcga
     3001 cgcaaagtta gcaggcgcgt atcctcgcag atctacgctc acgatgcgac aatttaatcg
     3061 gttcttcatc gggtggtggg tcaggcatgg gttgcgggcg agggatcgga tcgggcactg
     3121 gaacaggatc gccaggaatc ggttcaggga caggaatttg caaataaata agtgtcgtca
     3181 tatttccctc cggtcattgg gtggactctt aaagggtaga cgctgataaa taacaggcaa
     3241 aaaaaagccg actcatcaaa gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca
     3301 aaaaaggaag taagacaata tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc
     3361 aagggagata ttgccctgaa tgggtagaga gtttattgac ttcgctcaaa ctttgcggcg
     3421 tttttgtata cagacagccg gaaaaattgc ttttgttaca accatttact acggtgcaac
     3481 cataaagcaa caccaccaat aagaacaact aacagaatac aaaaaattga aaatccgaat
     3541 tgccacccgc cgccagggat cccaccaagg ttgacgccaa ataacccggt cagaaaggta
     3601 ctgggtaaaa agaccattgc catcaacgac attgtatagg tacgacgagc taaattttcc
     3661 tgcatcacct gagcgatttc atccgccatc acgccagtcc gtgctataca ggcgtcgatt
     3721 tcgtcaaggc cgcgcccaag gcgatcggca atatcctgca tccgacggcg ttggtcatcg
     3781 ctcatccacg gcaaacgttc actggcaaga cgagcataaa catcacgttg cggtgccata
     3841 tagcgacgca tcacaattaa ttgtttgcgc agcagagcca ggaatccacg cggtggaatt
     3901 tgctgatcaa ggagattatc ttcaaggtcg ataattttat cgtgcagctg ctcgataaat
     3961 tcgctggaat gatcggtcaa cgcatcgcac acatccacca gccaccctcc gcaatcggtc
     4021 ggacccgtgc cctcttccag atcgctcacc acatcgtcca gcgccagcac tttgcgttgt
     4081 cgggtcgaaa caattaaccg cccgtccata tatacacgca tggcgaccag ttgatcgggg
     4141 cgttcatcgg tgctgccgtt tatacagcgc aatgtaatca gcgtgccttc accgagacgg
     4201 ctgactcggg gccgcgtgct ctcgcccgcc agcgcatcac gtacgttatt gggaagcagc
     4261 ggtgttgtcg ccagccattg ggcgctatca tggtgtacat aattaaggtg gagccagcag
     4321 ggatgcgctt catcaatcac atctgtattt tccagcggtt taacgccgcc tctaccatcc
     4381 agcatccagg caaatactgc atccgggaca ttaacgtccg atcccttaat cgcttccaca
     4441 gtgcctccat catcaacgca ttattttgta gtctagcctt ctggccctgt tacgcaacat
     4501 ctcatcaccc cattaccctg aaatgattaa taaaattctg tctaaattga atacaaaaag
     4561 caaaatgctt ttccgtatac aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga
     4621 attatctgat catatgacgt ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa
     4681 taacgggatc tcaatgatta cgcacaactt caataccctg gacttactca ccagtcctgt
     4741 ctggatcgtt tcgccctttg aggaacagtt aatttatgcc aatagcgcgg cgcgactgtt
     4801 gatgcaagac ctcacgttta gtcagctacg aaccggtccc tattccgtct cctcacaaaa
     4861 agaactgccg aaatacctct ccgatctgca aaaccaacac gatattatcg aaatcctcac
     4921 tgttcagcgt aaagaagagg aaacagcatt aaactgtcgg cttgttttgc gaaagctgac
     4981 agaagcagaa ccggtgatta ttttcgaagg tatcgaagcg tcggcaacgc tgggtttaaa
     5041 agccagtcgc tcggcaaatt atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct
     5101 gactaactct gcaccaatgt tgttgattga cccctcacga gatggccaaa tcgtcgatgc
     5161 taacctcgcc gcgctcaatt tctatggtta taaccatgaa acgatgtgcc agaaacatac
     5221 ctgggaaata aatatgctcg ggcgtcgcgt catgcctatc atgcatgaaa tttcgcattt
     5281 acccggtggt cataaacctc tgaattttat tcataagctg gcggatggtt cgactcgtca
     5341 tgtgcagacc tacgccgggc cgattgaaat ttatggcgac aagctcatgt tatgtattgt
     5401 acatgatatt actgagcaaa aacggctgga ggagcagctg gaacatgctg cccaccatga
     5461 cgcgatgacc ggattactga atcggcgaca gttttatcac attaccgagc caggccaaat
     5521 gcagcatctc gccatcgccc aggattacag cttgttgctc atcgacaccg atcgttttaa
     5581 acacattaac gatctctatg ggcattctaa aggtgatgag gtgttatgcg ccctcgcccg
     5641 caccctcgaa agttgcgctc gcaaaggcga tttggtgttt cgttggggag gcgaagagtt
     5701 tgtcttattg ctaccaagaa ccccactgga taccgcgctt tcgctggctg aaactatccg
     5761 cgtaagcgtg gcaaaagtga gtatttcggg cttaccacgc tttaccgtca gcattggtgt
     5821 ggcgcatcac gaaggaaatg aaagcatcga tgaactgttt aaacgcgttg atgatgcttt
     5881 gtatcgggcg aaaaatgatg gacgcaaccg cgtgctggcg gcataagccg cggatgcgtc
     5941 tcgagatcaa cgactgcgct tagcgtggcg ctcccagttt tcttgcttcg cctgcgccgt
     6001 tttacgtagt gcgacgtaac acgccccgct gccgccatga tgcggtagcg cagtgcaata
     6061 tgcctgaaca tcatcaaatt cggtcagcca gcgcgccaca tagctgcgga caatattggc
     6121 atgcgattta tcatcccgtc ctttgccatg aataatcagc acgttacgca aaccatcagc
     6181 catggcttgt tgaatgaaac tgaacatcat tttgcggcac tcttccaccg gctggcgcaa
     6241 aagattcagg ctcgcctgtt gcggatattt accactgcgc agcttatcca gcaccccatg
     6301 ttgcaaccct tcccgccgaa actccagcgg ctgacttagt gggatgatgt cgagaaatcc
     6361 ggtggtgagg aaattatcaa gctgcagcgt gtcgatacgc tgcggcgcac gttggttacg
     6421 cgttggatgc cagtggacat cggtagcacg tttcagcggc tggacatctt ccatggcgtc
     6481 aagaaacagc gatttgtcgt caaggttcat gttacatcct cccgcaatta agagcgcgat
     6541 atgataacca gaccgggtcg gtccaacaac gtattaccca aaattccagt aataagttcc
     6601 aaatattgcc gatattttaa gcaaaatgct tatgcatgat tattcattca cgatattaat
     6661 aatgtaactt atattttcgt gaaatctgtc actgaagaaa attggcaact aaaggttaaa
     6721 accgttataa cacagtcacc ggcgcagagg agacaatgcc ggatttaaga cgcggatgca
     6781 ctgctgtgtg tactgtagag tctggcggat gtcgacagac tctatttttt tatgcagttt
     6841 taactttgca gatagccgca ttctcgccgg aattgcgtga ttaatgacgc ggtcagtggt
     6901 gtctggcgac tatcacgccg ctgaatcaaa taataggccg ctttcggtaa aatttcgcta
     6961 accggcaaca tcaccagccc ctgtccgtgc aagggatcgc agcccatttc ttcaggcagt
     7021 atgctgagaa aatcgctttt tgccaccaga ctgatacagg ctgagaacgt ctcgcagact
     7081 acaccgacct gtggcgtttg cgcctgatcg tcaagcaatt cactcaactg tttgtagtaa
     7141 ctgccgtgtg gcgtcggcat tgtccagctg taatccagta actgtttgat cgaacgggca
     7201 ccaatggcgg ggtgtcccgg gcggcaaaag atcgcgaatt gcttttccag taatttctca
     7261 aaagtaaatt cgtggtcgta cggtccctga taataggtat tgatggtgaa atccaattct
     7321 ccctgacgca attcattaat catcgacacc agttgccctt ccataatgcg tacttttacc
     7381 tgcggatgct gctgatgaaa acgagatatg acagctggca tcagactgcg ggaaatactg
     7441 gcccccatgc cgatattaat ctgccctgcc agttgccctt gtcgttggcg aatatcctct
     7501 tgggctgcgc gcagctcttc aagaataaga ctggcgtgct gataaaaact ttcaccggca
     7561 tcagttaacg tcacgccttt actacggcga aaaaagagtt gcgccgctaa tccttcttcc
     7621 agctcctgaa tagatttact cagtgccggt tgcgacatat tcaacattcg gctcgctccg
     7681 cgaatgctgc cctgacgagc cacttcaaca aaagcccgaa tttgatgaat ttttacctga
     7741 aaagccatga cgccaccgat aaccgttatt tatcagacca aagaaactgg catctacttt
     7801 aatgcagatg attgtgtcag ggtaatttat gaacggttaa aactgtgaaa aatcagttag
     7861 tgataagtaa aaactatcgc tacgtgaacc gggtcacact ttttactgat gacgggaaag
     7921 gttatggagt ctttgaatca atttgttaat tcgcttgccc caaaattatc gcactggcga
     7981 cgtgattttc atcactatgc agagtctggc tgggtggaat tccgcactgc cacccttgtt
     8041 gcggaagaat tgcagcagct cggctattca ctggcgctgg gccgcgaagt agttaatgaa
     8101 agtagccgga tgggactacc tgatgaactc actctacaac gcgaattcga gcgcgctcgt
     8161 caacagggtg cgctagcaca atggattgcg gtttttgaag gtggtttcac tggtatcgtc
     8221 gccaccctgg ataccggtcg ccccggtccg gtgatggctt tccgtgtcga tatggacgcg
     8281 ctggatctca gtgaagagca ggatgtcagc catcgcccct accgcgacgg ttttgcgtca
     8341 tgtaacgccg gaatgatgca tgcctgtggt catgatggac ataccgccat tgggcttggg
     8401 ctggcgcata cccttaaaca attcgagtcc ggactacatg gcgtcatcaa actgattttt
     8461 cagcctgcag aggaaggtac gcgtggcgcg cgggcgatgg tcgatgcagg tgtcgtagat
     8521 gatgttgatt attttactgc cgtgcacatt ggcactggcg tacctgcggg caccgtggtg
     8581 tgcggcagtg ataattttat ggcaaccacc aaatttgacg cgcacttcac cggtaccgcc
     8641 gctcacgcag gcgcaaaacc agaagacggt cacaatgcct tgttggcggc agcacaagcc
     8701 actcttgcac tgcatgcaat cgccccgcac agcgaaggag cttccagagt aaacgtgggc
     8761 gttatgcagg caggaagcgg tcgtaacgtt gttcctgcct cggcgttgct gaaagtggaa
     8821 acacgcgggg ccagcgacgt cattaatcaa tatgtttttg accgtgcaca acaagcgatt
     8881 cagggcgcag caaccatgta tggtgtcggc gttgaaactc gtctgatggg tgcagctacc
     8941 gccagttctc cttcgccgca atgggtcgca tggttgcaaa gtcaggcggc tcaggtcgcg
     9001 ggggtcaatc aggccattga acgtgttgaa gcgcctgcgg gttccgaaga tgccacatta
     9061 atgatggccc gcgtgcagca acatcaaggg caagcctcct acgtggtgtt tggcacacag
     9121 ctggcggcag gtcatcacaa cgaaaaattc gattttgacg agcaggttct cgctattgcc
     9181 gtcgaaacgc tggcgcgcac cgcgctcaat tttccctgga cgcgaggtat ctgatgcagg
     9241 aaatctatcg ttttatcgac gatgcgattg aagccgatcg ccaacgttat accgatattg
     9301 ccgatcaaat ctgggatcat ccagaaacac gttttgaaga gttctggtca gcggagcatc
     9361 tggcttcggc tctggaatct gcaggcttca ccgttacccg caacgtaggc aatatcccaa
     9421 atgcctttat tgcttcgttt ggtcaaggca aaccggttat cgccctgctg ggagaatatg
     9481 acgccctggc aggtttaagt cagcaagctg gttgcgcgca acctacatcc gcgacgcccg
     9541 gtgaaaatgg tcacggttgc ggacacaatt tgcggggaac cgccgccttt gccgctgcaa
     9601 tagccgtcaa gaaatggctg gaacaatatg ggcaaggcgg cacagtgcgc ttttatggtt
     9661 gtcctggcga agaaggcggc tcgggtaaaa cgttcatggt ccgcgagggg gtatttgatg
     9721 atgtggatgc ggcactcacc tggcacccgg aagcctttgc cggtatgttc aatacccgca
     9781 cgctggcaaa cattcaggca tcatggcgct ttaaagggat cgcagcacat gccgcgaatt
     9841 cccctcattt gggacgcagc gcccttgatg ccgtaacgtt gatgaccact ggcaccaact
     9901 tcctcaacga acatattatt gaaaaagcgc gcgtacacta tgccatcaca aatagcggcg
     9961 ggatctcgcc caacgtggtc caggcgcagg cagaagtgct ttatcttatc cgcgcccccg
    10021 aaatgaccga cgtgcagcat atttatgatc gggtcgccaa aatcgccgaa ggtgcggcat
    10081 tgatgaccga aaccacggtt gaatgccgct tcgacaaagc ctgttccagt tatctcccga
    10141 atcgcacctt agaaaatgcc atgtaccatg ccctatccca ttttggtacc ccggaatgga
    10201 actccgaaga actggctttt gcgaaacaaa ttcaggctac gctcaccccc aacgatcggc
    10261 aaaacagtct gaataatatc gccgcaaccg gtggcgaaaa cggcaaggct tttgcactac
    10321 gtcatcgtga aacggtactg gcgaatgagg tcgctccata tgccgccacc gataacgtgc
    10381 ttgcggcatc gactgatgtc ggcgacgtta gttggaaact gcctgttgcc cagtgtttca
    10441 gcccctgttt tgccgtcggt acaccgctac atacgtggca actggttagc caggggcgaa
    10501 catctattgc tcataaagga atgctgctgg cggcgaaaac tatggcagca accacagtca
    10561 atctcttcat tgattcaggg ctattgcaag aatgccaaca agagcatcag caagtaacgg
    10621 acacgcaacc gtatcactgc cctatcccga aaaacgtgac accgtcacct ttaaaataac
    10681 aacaacaacg caaacacaac aaccgaggaa tgcccatgag tatgtcatcc ataccgtcgt
    10741 cctcccaatc cgggaagctc tatggctggg tcgaaagaat tggtaacaag gttctccatc
    10801 cttttttgct ctttatctat ttgattatcg tactcatggt gacgacggca attttgtcgg
    10861 cctttggcgt cagtgcgaaa aacccgaccg atggaacgcc ggtggtggtg aaaaacctgc
    10921 tcagtgtgga aggattacac tggtctttac ccaatgttat taaaaacttt agcggttttg
    10981 ctccacttgg tgcgatcctg gcgctggttt taggtgccgg tctggcggag cgcgtcggct
    11041 tactgccagc gctaatggtt aaaatggcat cgcatgttaa tgcccgctac gccagttata
    11101 tggtgctgtt tattgctttt ttcagccaca tttcttccga tgcgacgtta gtgatcatgc
    11161 caccgatggg tgcgctgatt tttctggcgg tgggcaggca tccagttgca ggtttactgg
    11221 ctgccattgc aggcgtaggt tgcggcttta cggctaattt actgattgtc acaaccgacg
    11281 tgttgctgtc ggggatcagc acggaagcgg cggctgcgtt caatccgcaa atgcacgtca
    11341 gtgtaattga taactggtat tttatggcca gctccgtagt cgtactgacg attgttggcg
    11401 gcctgataac cgacaaaatc atcgagccac ggttaggtca atggcaggga aacagcgatg
    11461 agaaactgca gacattgacc gaaagtcagc gttttggttt acgcatagca ggtgtcgtat
    11521 cgctactttt tattgctgcg attgcgctga tggtgatccc ggaaaacggg atattgcgcg
    11581 atccgattaa tcacaccgtg atgccatcac cctttattaa aggtatcgtg ccactgatca
    11641 ttcttttttt ctttgttgtc tcgctggctt atggcatcgc tacccgcaca attcgacgtc
    11701 aggcggattt accgcagtta atgattgaac cgatgaaaga gatggcagga tttatcgtga
    11761 tggtttttcc cctcgcccag tttgtcgcca tgtttaactg gagcaacatg g
//
LOCUS       DAZRUF010000127        11661 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZRUF010000127 DAZRUF010000000
VERSION     DAZRUF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11661
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.127"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            346..1734
                     /gene="narU"
                     /locus_tag="ACHXOL_002708"
     CDS             346..1734
                     /gene="narU"
                     /locus_tag="ACHXOL_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR6809651.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1816..5556
                     /gene="narZ"
                     /locus_tag="ACHXOL_002709"
     CDS             1816..5556
                     /gene="narZ"
                     /locus_tag="ACHXOL_002709"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR6809652.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5553..7097
                     /gene="narH"
                     /locus_tag="ACHXOL_002710"
     CDS             5553..7097
                     /gene="narH"
                     /locus_tag="ACHXOL_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR6809653.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7097..7792
                     /gene="narW"
                     /locus_tag="ACHXOL_002711"
     CDS             7097..7792
                     /gene="narW"
                     /locus_tag="ACHXOL_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR6809654.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7789..8469
                     /gene="narI"
                     /locus_tag="ACHXOL_002712"
     CDS             7789..8469
                     /gene="narI"
                     /locus_tag="ACHXOL_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR6809655.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8548..9441
                     /locus_tag="ACHXOL_002713"
     CDS             8548..9441
                     /locus_tag="ACHXOL_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR6809656.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9537..10382)
                     /gene="nhoA"
                     /locus_tag="ACHXOL_002714"
     CDS             complement(9537..10382)
                     /gene="nhoA"
                     /locus_tag="ACHXOL_002714"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR6809657.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10555..11124
                     /locus_tag="ACHXOL_002715"
     CDS             10555..11124
                     /locus_tag="ACHXOL_002715"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR6809658.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11128..11355)
                     /gene="pptA"
                     /locus_tag="ACHXOL_002716"
     CDS             complement(11128..11355)
                     /gene="pptA"
                     /locus_tag="ACHXOL_002716"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR6809659.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11455..>11661)
                     /locus_tag="ACHXOL_002717"
     CDS             complement(11455..>11661)
                     /locus_tag="ACHXOL_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR6809660.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMGRNYLASVLTGSGLS"
BASE COUNT         2732 a         3023 c         3064 g         2842 t
ORIGIN      
        1 cctccagttc atggcctttt tagtgaataa atcatcacta attctataag tgtctggtct
       61 tgttgataat atagttaata tgtaaccctt tagattcacg atctgaaggg ttgaaacatg
      121 acatacgttc aaagattaac cccgtttgaa ggttagcttg ttagttacat ttagtaacac
      181 atatctggct acttaatccc cattctgccc ccgcggttac gctttcttat ctattctttc
      241 aatcagcact tcacccaatt catccttaat tctaataact ggctattgcg ccttacctcc
      301 tgttttgtct tttttcatta ccaatgtgtg catgtgagga acaatatggc actgcaaaat
      361 gagaaaaata gtcgttatct tttacgcgac tggaaaccag aaaatccggc cttctgggaa
      421 aataaaggaa aacatattgc tcgaagaaac ctctggatat cagtcagttg tctacttctt
      481 gccttctgtg tctggatgct atttagcgca gttaccgtta atctcaataa aatcggtttt
      541 aattttacta ccgatcaact ctttttatta accgcattac cctccgtttc tggcgcatta
      601 ttgcgtgttc cctactcctt tatggtgcct atattcggtg gacgccgatg gacggttttt
      661 agtactgcaa tcctgattat tccttgcgtc tggctcggaa ttgccgtgca aaatccgaat
      721 actccttttg ggatatttat cgttatcgct ttgctatgcg gttttgcagg tgcaaacttt
      781 gcttcgagca tgggcaatat cagtttcttc tttccaaaag ccaaacaagg gagcgctctt
      841 gggattaatg gcggattagg aaacttaggt gtaagtgtaa tgcagctggt tgcaccgctg
      901 gtcatttttg tacctgtatt tgcctttctc ggcgtcaatg gcgtaccgca ggccgacggt
      961 tcggtgatgt cgctggcgaa tgccgcatgg atttgggtac cgctactggc gattgccacg
     1021 atcgccgcct ggtcagggat gaatgatatc gccagttcac gcgcctcaat tgacgaccag
     1081 ctccctgtct tacaacgcct gcatctctgg ctgctgagcc tgctttacct tgccaccttc
     1141 ggttcgttta tcggtttttc tgcgggtttt gccatgctgg caaaaaccca gttcccggat
     1201 gtgaatattc tgcgcctggc gttctttggc ccatttatcg gtgccatcgc gcggtcggtt
     1261 ggtggtgcta tttccgataa attcggcggc gtgcgggtga cgttgatcaa ctttattttt
     1321 atggcgattt tcagtgccct gctgttcctt accttaccgg gcacaggctc cggtaatttc
     1381 atcgcctttt acgccgtatt tatggggctg tttctgactg cgggtctggg aagtggttct
     1441 actttccaga tgatcgccgt catctttcgc cagataacca tttatcgggt gaagatgaaa
     1501 ggcggtagtg atgagcaagc tcagaaagaa gccatcaccg aaacggcggc ggctctgggc
     1561 tttatctcag ccattggcgc agtgggcggc ttttttattc cgcaggcgtt tggcatgtcg
     1621 ctcaatatga ccggctctcc ggtcggcgcg atgaaagtgt ttttaatctt ctacatcgtt
     1681 tgtgtgctgc tgacctggct ggtttatggt cggcggaagt tcagccaaaa ataagagatc
     1741 gatgcctgat gtacaaccgt gtcgcatccg acaaggcatc agatcccaat tcattcctgg
     1801 agcaggagtc atgtcatgag taaacttttg gatcgctttc gctacttcaa acaaaagggc
     1861 gaaacctttg ccgatggtca cggacaggtg atgcatagca accgcgactg ggaggacagc
     1921 tatcgccagc gttggcagtt cgacaaaatc gtgcgttcca cccacggtgt taactgtaca
     1981 ggctcctgta gctggaaaat ctacgttaaa aatggtctgg tgacctggga aatccaacag
     2041 accgactacc cgcgcactcg ccctgaccag cccaatcatg aacctcgcgg ctgcccgcgt
     2101 ggcgcaagtt actcctggta tctctacagc gctaaccgcc tgaaataccc gctcattcgt
     2161 aaacgactga ttgaactgtg gcgtgaagct ctcaagcaac acagcgatcc ggtactggcg
     2221 tgggcatcga ttatgaacga tccgcaaaag tgcttaagct acaaacaagt gcgtgggcgc
     2281 ggcgggttta tccgctccaa ctggcaggaa cttaaccaac tgattgccgc cgctaacgtc
     2341 tggaccatca aaatctatgg tccggatcgc gttgccggtt tctcgccgat cccggcgatg
     2401 tcgatggtgt catacgctgc cggaacgcgt tatctgtcgc tgttaggcgg cacctgttta
     2461 agcttctacg actggtattg cgacctgccg cccgcctcgc cgatgacctg gggcgaacaa
     2521 accgacgtgc cggaatctgc cgactggtat aactccagtt acatcatcgc ctgggggtct
     2581 aacgtaccgc agacacgtac gccggacgcc cacttcttta ccgaagtacg ttacaaaggc
     2641 accaaaacta tcgccattac ccctgactac tctgaagtgg ccaaattgtg cgaccagtgg
     2701 ctggcaccga aacaaggcac tgatagcgcc ctggcgatgg caatgggcca tgtgatttta
     2761 aaagagtttc atctcgataa tcccagcgac tactttatca actactgccg ccgctatagc
     2821 gacatgccga tgctggtaat gctggaacct cgcgacgatg gtagctacgt acccgggcgc
     2881 atgatccgcg cgtctgacct ggtagatgga ctaggcgaaa gcaataatcc gcagtggaaa
     2941 accgtggcgg ttaataccgc aggtgaattg gtagtgccga atggttcgat tggtttccgc
     3001 tggggagaaa aaggcaaatg gaatctggaa tccattgccg ccggtaagga aaccgaattg
     3061 tcgttaaccc tgctcggtca acatgacgct gttgcaggcg tggccttccc ctactttggc
     3121 ggcattgaaa atccgcattt tcgcagcgta aaacacaatc cggtgctggt gcgccaattg
     3181 cccgttaaaa acctgacgtt agtcgatggc aacacctgtc cggtggtcag cgtttatgat
     3241 ttggtgctgg cgaattacgg cctcgatcgc gggctggaag atgaaaacag cgcgaaagat
     3301 tacgctgaaa tcaaaccgta caccccagcc tggggtgagc aaattaccgg cgtaccgcgc
     3361 cagtatattg aaaccatcgc ccgcgaattt gccgataccg cccataaaac gcatggacgc
     3421 tcaatgatta tcctcggcgc tggcgtcaac cactggtatc acatggatat gaactaccgt
     3481 gggatgatca atatgctgat cttctgcggt tgtgtcgggc aaagcggcgg cggctgggcg
     3541 cactatgtcg gtcaggaaaa actgcgccca caaaccggct ggttgccgct ggcctttgcg
     3601 ctcgactgga accgcccacc gcgccagatg aacagtacct cgtttttcta caatcattcc
     3661 agccaatggc gctatgaaaa agtctctgct caggagttac tttcaccgct tgccgatgcc
     3721 agtaagtaca gcggtcatct gattgatttc aacgttcgcg ccgaacgcat gggctggcta
     3781 ccttctgcgc cgcagctggg gcgtaacccg ctcgggatta aagctgaagc cgacaaggcc
     3841 ggattatccc ccacagaatt taccgcccag gcgctgaaat cgggcgattt acgtatggcc
     3901 tgcgaacaac cagatagcgg cagcaatcat ccgcgtaatt tgtttgtctg gcgctctaac
     3961 ctgcttggct cctccggcaa aggccacgag tatatgcaga agtatctgct ggggaccgaa
     4021 agcgggattc agggcgagga actcggtgcc agcgacggga tcaaaccgga agaagtcgag
     4081 tggcaaactg cggcgattga aggcaagctc gacctgctgg tgacgctcga cttccgcatg
     4141 tccagtacct gcctgttctc cgatatcgtt ctgcccaccg ccacctggta cgaaaaagac
     4201 gatatgaaca cctcggatat gcatccgttt attcatccgc tttctgcggc ggtcgatccg
     4261 gcgtgggaat cacgcagcga ctgggaaatc tacaaaggta tcgccaaagc attttcgcaa
     4321 gtgtgcgtgg gtcatcttgg caaagaaacc gacgtggtat tacaacccct actgcatgac
     4381 tctccggcag agctctcaca gccgtgtgaa gtgctcgact ggcgcaaagg cgaatgcgat
     4441 ctgattccag gcaaaaccgc gccgaatatt gtggcggtgg agcgcgacta ccctgctacg
     4501 tatgaacgct ttacctcgct cgggccattg atggacaaac ttggcaatgg tggtaaaggg
     4561 atttcgtgga atacccagga tgaaatcgat ttcctcggca aactcaatta caccaagcgt
     4621 gatggcccgg cacaggggcg tccgctgatt gacaccgcca ttgacgcttc agaagtgatt
     4681 ctggcactgg caccggaaac caacggtcat gttgcagtca aagcgtggca ggcgctgggc
     4741 gagatcaccg ggcgcgaaca tacccatctg gcgctgcaca aagaggacga gaagattcgc
     4801 tttcgcgata ttcaggcgca gccgcgtaaa attatctcca gccccacatg gtccggtctg
     4861 gaaagcgatc acgtctccta taatgcggga tacaccaacg ttcatgagtt aattccgtgg
     4921 cgcacgctgt cgggacgcca gcagctctat caggatcatc cgtggatgcg tgcttttggt
     4981 gaaagcctgg tggcttatcg cccgcctatc gacacccgta gcgtcagtga gatgcgccag
     5041 atcccgccaa acggcttccc ggaaaaagca cttaacttcc tgacgccgca ccagaaatgg
     5101 ggcattcact caacctacag tgaaaacctg ctaatgctga cgctctctcg cggtggaccg
     5161 attgtctgga tcagcgaaac cgatgcccga gaactgacca ttgtcgataa cgactgggtg
     5221 gaagtcttta acgccaacgg cgcgctgacg gctcgtgcgg tggtcagcca acgtgtaccg
     5281 ccgggtatga ccatgatgta tcacgcccag gaacgcatta tgaatattcc tggttcggaa
     5341 gtaactggca tgcgcggcgg cattcataac tcggtcaccc gtgtttgccc gaaaccgacg
     5401 catatgattg gcggttacgc gcagctggcc tggggcttta actactacgg caccgtcggc
     5461 tcgaaccgcg acgaattcat catgatccgc aagatgaaga acgttaactg gctggatgat
     5521 gaaggtcgcg atcaggtaca ggaggcgaaa aaatgaaaat acgttcgcaa gtcggcatgg
     5581 tgcttaacct cgacaaatgt atcggttgcc atacctgttc ggtgacctgt aaaaacgtct
     5641 ggaccggacg cgaaggcatg gagtacgcat ggtttaacaa cgtcgaaacc aaaccgggca
     5701 ttggttatcc gaaaaactgg gaagatcagg aagagtggca aggcggctgg gtccgcgatg
     5761 tgaatggcaa gatacgcccg cgtctgggca gcaagatggg cgtaataacc aaaatcttcg
     5821 ccaacccggt ggtgccgcag attgatgatt actacgaacc tttcaccttc gactacgaac
     5881 atttgcatag cgcaccggaa ggcaaacata tccctactgc tcgcccgcgt tcgctgattg
     5941 acggtaagcg gatggacaaa gtgatctggg ggccaaactg ggaagaactg ctgggcggcg
     6001 agtttgaaaa acgtgcccgc gaccgcaact tcgaggccat gcaaaaggag atgtacgggc
     6061 agtttaaaaa caccttcatg atgtacctgc cgcgcctgtg cgaacactgc ctcaatccca
     6121 gttgcgtggc gacctgccca agcggcgcta tctacaaacg cgaagaagac ggcattgtgc
     6181 tgattgatca ggataaatgc cgtggctggc gtttgtgcat tagcggttgt ccgtacaaaa
     6241 aaatctactt caactggaaa agcggcaagt cagaaaaatg tatcttctgt tatccgcgaa
     6301 ttgagtcagg ccaacccacc gtgtgctcag aaacctgcgt gggtcgcatc cggtatctgg
     6361 gcgtgctgct ttacgacgcc gaccgcattg aggaagcggc gagcaccgag cgcgaagttg
     6421 acctctatga acgtcagtgc gaagtgttcc tcgatccaca cgatccctca gtgatcgagg
     6481 aagccctgaa acaaggtatt ccacaaaacg tgattgaagc tgcccagcgt tcgcctgtct
     6541 acaaaatggc gatggactgg aaactggcgc taccgctgca ccctgaatat cgcaccctgc
     6601 caatggtctg gtacgttcct ccgctgtcgc cgattcagtc ctacgcagat gcgggcggtt
     6661 tgccgaaaag cgaaggcgtg ctgcccgcca tcgaaagcct gcgtattccg gtgcaatatc
     6721 tcgccaatat gttgagtgcc ggcgataccg gtccggtact gcgggcgctg aaacggatga
     6781 tggcgatgcg ccactatatg cgttcacaaa ccgtggaagg cgttactgat actcgtgcca
     6841 tcgacgaagt aggcctgagc gtcgcccagg tcgaagaaat gtatcgctat ctcgccattg
     6901 ccaactatga agatcgtttt gttatcccga ccagccatcg ggaaatggcg ggcgatgcct
     6961 tcgcagaacg caacggctgc ggttttacct ttggcgacgg ttgccacggt tcggacagta
     7021 aattcaacct gttcaacagt agccgtatcg atgccatcaa catcaccgaa gtgcgcgaca
     7081 aagcggaggg cgaataatgc agatcctcaa agtgatcggc ctgttgatgg agtatccgga
     7141 tgagctgttg tgggaatgca aggaggacgc gctggcgttg atccgccgcg acgcgccgat
     7201 gcttacggat ttcacccaca acctgcttaa cgcgccgctg ctggataaac aggccgaatg
     7261 gtgcgaagtg tttgaccgcg ggcgcaccac gtcgctgctg ctgttcgaac atgttcatgc
     7321 cgagtcccgc gatcgcggcc aggcaatggt ggacctgctg gcggagtatg aaaaggtcgg
     7381 cctgcagctg gattgtcggg aactgcccga ttatctaccg ctgtatctgg agtatttaag
     7441 cgtactgcct gacgatcagg cgaaagaagg attgctcaat gttgcgccga tcctcgccct
     7501 gcttggcggt cgcttaaaac aacgcgaggc accgtggtac gcgttgtttg atgctctgct
     7561 gcaactggcg ggaagtatac tttcaagtga cagtgtcaca aaacaagtaa acagcgaaga
     7621 gcgcgatgac acccgccagg cgcttgatgc agtgtgggaa gaggaacagg tgaagtttat
     7681 tgaagataac gccacggcgt gtgacagctc gccgcttaat caatatcagc gacgctttag
     7741 ccaggatgtc gcgccgcagt atgtcgacat cagtgcggga ggtgggaaat gattcagtat
     7801 ctgaacgtct ttttttacga tatctacccg tacatttgtg cgacggtgtt tttcctcggc
     7861 agctggctgc gctacgacta cgggcagtac acctggcgcg cctcctcaag tcaaatgctc
     7921 gataaacgcg ggatggtgat atggtcgaat ctgttccata tcggcatttt ggggattttc
     7981 ttcgggcact tgttcggcat gttaacgccg cactggatgt acgcgtggtt tttgccagtg
     8041 gcagcgaaac aactcatggc gatggtgctc ggcggtattt gcggtgtgtt gacgttgatt
     8101 ggcggcgcag gactgctgtg gcgcagtctg acaaatcagc gcgtgcgtgc cacttccact
     8161 acgccggata tcatcatcat gagcattttg ctgatccagt gtctgttggg gctaagtacc
     8221 ataccgtttt cagcacagta tcctgatggt agcgaaatga tgaagctggt gggttgggcg
     8281 caaagcattg tcactttccg tggtggatcg tcagagatgc tcaacggcgt agcgttcgtc
     8341 ttccgcctgc atctggtgtt gggaatgacc atcttcctgc tcttcccgtt cacccgactg
     8401 gtgcatgtgt ggagcgcgcc gtttgagtac tttactcgtc gatatcaaat tgtgcgttcg
     8461 cggcgataat ttcaccctgg ccggatagcc cttcgctctc cggccacttg caggctatga
     8521 tagttctcca gtcacaggag aacgttaatg aaaccgcagg tgtaccacgt cgatgctttt
     8581 acctctcaac catttcgcgg caattctgcc ggtgttgttt tccccgccga taatcttagc
     8641 gaagcacaaa tgcagcttat cgcccgcgag ttaggccatt cggaaactgc ttttctgctg
     8701 cacagcgacg acagcgatgt gcgcatccgc tactttacgc caacggttga agtgccgatt
     8761 tgcggtcacg cgacagtagc tgcgcactat gtacgtgcga aggtgttagg tttaggaaat
     8821 tgcacgatct ggcaaacatc gctggcagga aaacatcgcg tgactatcga aaagcacaac
     8881 gatgattatc gtatttcgct ggaacaaggt acgccgggct ttgaactgcc gctggagggt
     8941 gaagtacgcg cggcgattat caacgcattg catcttactg aagacgatat tctgccgggt
     9001 ttgccgatcc aggtcgcgac gaccgggcat tctaaagtga tgatcccatt gaagccggaa
     9061 gtggatatcg acgctctttc gcctgacctt aatgcgctga ccgctatcag taaacagatt
     9121 ggctgcaatg gtttcttccc gttccagatc cgtccaggca aaaacgaaac cgatggtcgt
     9181 atgttctcgc ctgcgattgg tattgtggaa gatccggtga ctggcaatgc caatggcccg
     9241 atgggtgcat ggttggtaca tcacaacgta ttgccccacg atggcaacgt gttgtgtgtt
     9301 aaaggccatc aggggcgcgc attggggcgt gacggcatga ttgaagtgac ggtgacaatt
     9361 cgtgataacc aaccggaaaa agtcaccatt tcaggcacag cggtgatttt gtttcatgcc
     9421 gaatgggcaa ttgaaatctg aaatcgttgc ctgatgcgct tcgcctgtca ggtctacaac
     9481 cgggctaatg tatgccggat aaggcgttta cgccgcatcc ggcaacctga cataaattat
     9541 tttcccgcct ccggatgcgt atcaaacgcc gccatcacca gcgctaactc atccacagta
     9601 aagccatgtt tcgcatcatc cacgcccaga ccaaactgtt cctgcatcac ggcatataac
     9661 gacgccacat ccggtagatt tcgctgctcc accgcgtgcc cattttcata atgggtaaaa
     9721 tgaaaattgg tcagtgtcag cttgccgccg tccggcaaat ggcggcacat cagcaaatga
     9781 tggcgaaaat gcgactgcgg ccagtgcgcc gaccagaaat tgcccatcac ataatcgctt
     9841 tgttgctgct cgcagagatc gaagcggtac atcggctgcc aatgctgatg atgattaaac
     9901 tgcaacaccc aatcatcacc ctcctgcaac aaccgatact ctccgtgtgg cgtggtctgc
     9961 acgagatcgg aaactaaacg aatcggcgcg gttagcgtct gcccaccgaa accgacatca
    10021 gcaatccatt tttccccttc cagttccacc aacagcaaac gatgggtgcg cggcggtaat
    10081 gctggcggat ttgataacac tacgcgccct aacaagctgc gaacgttaaa ccccagctcg
    10141 cgtaacaccc gctcaaacac gccattctgc tcaaaacagt aaccgccacg acgggctatc
    10201 accagtttct cttccagcga ttgatcatca agctgtattt ccctcggcag caaaacgtca
    10261 aggttttcaa acggaatggt gcaattgtgt ttcaggtgca atgcacgaag cgtatcaata
    10321 ttgaccgcag cagctcccga ccagttaata cgggcaaaat agtgattcag aatgggcgtc
    10381 atcaggcgtt tcccttagtg gttttctcaa cttataacct ggaatagacg ccgcaccggc
    10441 tattttgtgc gatcgcggac atgcatcacg tttgcaaatc gccaatcccg ttaatttcgt
    10501 tacttattgc gttacggact acgcttaaaa aaaccttctt cgcgaggcga taccatgagc
    10561 cgattcatcc ccatagaatt acaccatgcc agccgtctgt taaatcatgg cccaactgtc
    10621 ttgatcacca gtttcgatga gcaatcccag cggcgtaaca ttatggctgc agcctggtca
    10681 atgccggtgg agtttgaacc tccacgcgtg gcaattgtgg tagataaatc gacatggacc
    10741 agggagttga ttgaacgtaa cggtaagttt ggcatcgtta tcccgggcgt tgcagcaact
    10801 aactggacgt gggcggtggg aagtgtgtcg gtgcgtgaag aagataaatt taattgctat
    10861 ggcattccgg tggtgaaagg tccggtgctt ggtttgccac ttgttgaaga gaaatgcctg
    10921 gcgtggatgg agtgccgcct gctacctgcg acttctgcgc aacaaaaata cgacacgctg
    10981 tttggcgagg tggtatcagc agcggcagac gcacgggtat ttgtcgaagg ccgctggcag
    11041 tttgatgatg ataagctcaa tacgttgcat catttaggtg ctggcacgtt tgttactagc
    11101 ggcaagcgtg ttacggcagg ttaagcatta catgctgtaa ccgggtttct ttatcaaagc
    11161 ctccatttgg ggctcgattt cggcatccca gatagcttgc caagattctg gttgaatctg
    11221 ctgtagagca atgcttatcg agctatcttt actgttcaga tggcgaataa taacgtcggt
    11281 aatatctgca gcaagtgctg ctttttgttg ttcgtccagt tcacgcggaa agcatttaat
    11341 gtcgatgtgc ggcatgatta cgctcctttt gaaagatgtc agtaaaatta acacacattg
    11401 tgaatgtcat gtgtccggta aagcgtttat acgacaataa tgcccagggc aagattacga
    11461 aagcccgctc cccgtaagga ctgacgccag gtagtttctg cccatggctg cttttcgcat
    11521 cttacgtctt aaccctgcct tgaatacctt atcattcgtc aaaatattaa tagcaatgtg
    11581 ccgtatccct gaaaataatt ctgctgcatt tcctcttctt attttgcagt cgtcttcatt
    11641 cattaccacg tccagacgcc a
//
LOCUS       DAZRUF010000128        11512 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZRUF010000128 DAZRUF010000000
VERSION     DAZRUF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.128"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..468)
                     /gene="sulA"
                     /locus_tag="ACHXOL_002718"
     CDS             complement(<1..468)
                     /gene="sulA"
                     /locus_tag="ACHXOL_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR6809661.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATR"
     gene            687..1316
                     /gene="sxy"
                     /locus_tag="ACHXOL_002719"
     CDS             687..1316
                     /gene="sxy"
                     /locus_tag="ACHXOL_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR6809662.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(1279..3441)
                     /gene="yccS"
                     /locus_tag="ACHXOL_002720"
     CDS             complement(1279..3441)
                     /gene="yccS"
                     /locus_tag="ACHXOL_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR6809663.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(3451..3897)
                     /locus_tag="ACHXOL_002721"
     CDS             complement(3451..3897)
                     /locus_tag="ACHXOL_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR6809664.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            4020..6074
                     /gene="helD"
                     /locus_tag="ACHXOL_002722"
     CDS             4020..6074
                     /gene="helD"
                     /locus_tag="ACHXOL_002722"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR6809665.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(6106..6564)
                     /gene="mgsA"
                     /locus_tag="ACHXOL_002723"
     CDS             complement(6106..6564)
                     /gene="mgsA"
                     /locus_tag="ACHXOL_002723"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR6809666.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(6660..7322)
                     /locus_tag="ACHXOL_002724"
     CDS             complement(6660..7322)
                     /locus_tag="ACHXOL_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR6809667.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7495..7908
                     /locus_tag="ACHXOL_002725"
     CDS             7495..7908
                     /locus_tag="ACHXOL_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR6809668.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(7953..8270)
                     /gene="hspQ"
                     /locus_tag="ACHXOL_002726"
     CDS             complement(7953..8270)
                     /gene="hspQ"
                     /locus_tag="ACHXOL_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR6809669.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(8328..9518)
                     /gene="rlmI"
                     /locus_tag="ACHXOL_002727"
                     /pseudo
     CDS             complement(8328..9518)
                     /gene="rlmI"
                     /locus_tag="ACHXOL_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            9613..9891
                     /gene="yccX"
                     /locus_tag="ACHXOL_002728"
     CDS             9613..9891
                     /gene="yccX"
                     /locus_tag="ACHXOL_002728"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR6809670.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(9888..10217)
                     /gene="tusE"
                     /locus_tag="ACHXOL_002729"
     CDS             complement(9888..10217)
                     /gene="tusE"
                     /locus_tag="ACHXOL_002729"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR6809671.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(10308..10967)
                     /gene="yccA"
                     /locus_tag="ACHXOL_002730"
     CDS             complement(10308..10967)
                     /gene="yccA"
                     /locus_tag="ACHXOL_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR6809672.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVNLLSILGFASR
                     D"
     gene            complement(11174..11261)
                     /locus_tag="ACHXOL_002731"
     tRNA            complement(11174..11261)
                     /locus_tag="ACHXOL_002731"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11225..11227),aa:Ser,seq:tga)
BASE COUNT         3034 a         2832 c         2964 g         2682 t
ORIGIN      
        1 tctcgtggcg tgagaggatg cgcttaccgg acgcataata aaccccatag cgttaccttc
       61 atttgccgca tcaacaagtt cagcatgctc ttcttcagtc aaatcatctg ccaaccaacc
      121 gatcaccaca ctgtaattgc ccgtgcgtaa agcgcgaacc attgactcca cagtgtggca
      181 aggggagagc tggctaatct gcattacttt cgttaagggt agcccagatg cctgaaccca
      241 ttcccgactc agtttttgtt gcggtgttaa ccagagttgc cagcgcgatt gctgaccgag
      301 ttgctgtaac aatggcaaca gtagaagttg cgtcatcatg ggctgatctt cgcgatagac
      361 aacttcactg ataagcccgg ctgtagtgtt ttccgtagag acacgcgcaa ttttacttgc
      421 tgcggatgag aacgacgaag aacgatgtgc atagcctgaa gtgtacataa tcaatccagc
      481 ccctgtgagt tactgtatgg atgtacagta catccagtga caacaaagat caaccctatt
      541 ttcggaaaga gcctcgcaaa ttttgtcgtt ggtgacggga aaacataaat taatcttgcc
      601 ccttaagaat aagttgccta ttttcgtagt taacggatcc gttaatgtga atcattcttt
      661 tatgttatga ttttaaaagg aattttatga aaagcctctc ctataagcgg atctataaat
      721 cacaagaata cctggcaacg ttgggcacaa ttgaataccg atcattgttt ggcagttaca
      781 gcctgaccgt tgacgacacg gtgtttgcga tggtttctga tggtgagttg tatcttcggg
      841 cttgtgagca aagtgcacag tactgtgtaa aacatccgcc tgtctggctg acatataaaa
      901 agtgtggccg atccgttacc ctcaattact atcgggttga tgaaagtcta tggcgaaatc
      961 aactgaagct ggtgcgtctg tcgaaatatt ctctcgatgc agcgctgaaa gagaaaagca
     1021 cgcgcaatac ccgggaaaga ctgaaagatt tgcccaatat gtcttttcat ctggaagcga
     1081 ttctcgggga ggtggggatt aaggatgtac gggcgttacg tatacttggg gcaaaaatgt
     1141 gttggttgcg actgcggcag caaaacagtc tggtgacaga aaagattctg tttatgcttg
     1201 aaggtgccat tatcggcatt catgaagctg cgctcccggt ggcacgccgc caggagcttg
     1261 cagaatgggc tgactctctt acgccgaaac aggagtttcc tgcggaactt gagtaatctg
     1321 gcgttgcaga cgaccaatct caggcagtaa tgcaatcaat aatccaactt gttgtacgac
     1381 cagaggttct ttgctgtctg cccgtggttc aagttgctgc atccgctgtt tcaggctagc
     1441 taatgcctca ttgacgcgtt cttcatcagc aggttgatga tgtaacgcgt catcaacata
     1501 gcaaactgcg tcatcaagaa acgccagaat ttcaggatta gttaactgct cccggtgagc
     1561 accgagggct gagatatagc tggtaaacgt atggttaagg cacagcaacc gaaacgcggc
     1621 ttcgcgaatt tgcggggtaa cgttcggctc gctggacata tttgatacca ccgacgccag
     1681 ctcagcatca cggttgtgtg catcgcggcg ggcaatacga tacgccagac ggttatcacg
     1741 cccctgatgg tattgctcca gtatggcatc gagataccga cagttggcct ctgtggcgcg
     1801 ttcgagcatg cgcggcagat tgcgaaactg ccagtcaggc cagatgtagc tcactgccgc
     1861 ccacgcaatg gcacaaccaa tcagcgtatc gattacgcga ggtaacgcta cttcaaaacc
     1921 ttcacccagt aagttaaaac acagtagcac caaaagtgtg atgaacatcg ttgcatgagc
     1981 gtattgcacg ttacggaagg caaaaaagag cacgccggta ataaccagca gcaccagctg
     2041 cccttccagt gatggcacaa accacagcac aggaatgcca atggcgatac ctaccagcgt
     2101 accaataatc cttaacttca ggcggtggcg cgtggcgtta tagtttggct ggcagacaaa
     2161 caaacttgtc agcaagatcc aatacccgtg atgcattccg gttatctgaa tgatggcgta
     2221 gccgaagcac aacaccagcg acattcttac cgcatgacgg aagagggcgg attccggcgt
     2281 gaagtgacgg ctaagacgca gccagatatc actcaacccg tgcgggctgt catcagcgag
     2341 ctcattttcg tcattattat ggggtagtgc ctgggcctgt tctgattcaa ttgttgccag
     2401 ttgggcatca atggcgcgta aattgttcag caaaaatccc agtgttttga gtaaatcggc
     2461 gggtgcgccg ttatcgcgca tccgctccag cgcagcatca atatgcgtaa aagcgcgctc
     2521 aaaatgcgga tcatgttgat aaggctgacg caacaaaata cagcgtgaca gttgctggca
     2581 cgcctggccc tgcatcgaca tcagccgctg aaaacggaac agcacgtcgc tgtggcgaaa
     2641 atgttcacgc aatgtttgat actgaatatg agaagagctg gcacgctcgt gaatatcctg
     2701 tgcgacaaag taataatgca gcgtgcgacg cgttccccgt tgaccacgat cgccacgtaa
     2761 gcgggtcagc agcgagagtt tcgtctgatt caatgtcgcc atcagcagac cgttggcgag
     2821 agccaaatcg tacagcggtg cctggctttc atcttcaata tcaggatcaa acatgcgcga
     2881 cttgagctca agataacgcg ccagttgttc atagcaacgc gccaggttgt cctgcagcgg
     2941 gcggaccggg aacagcagat gaccaataag tgtcaggacg ttgtaccaga cggcaccggc
     3001 cagcagatac atcggctgct gataccagtg ctcatacagt gatgttccca acatagtgta
     3061 aatggcgatc agcaatgcac cgaaggcaat tgttgcatag cgttgaccca gaccgccgag
     3121 caaaatgaag ccgctggtag agagcgttaa gccaatcgca aatagccagg gccagggaaa
     3181 cagcaattct actgaggccg aggcgataaa aaagcagaac agcgtaatga tgaggttacg
     3241 caaacgtccc gccagtcggt catcgagatc ggtcagcgcc gctgccacca tccccagcgt
     3301 tagcggaatc gtcagtttta catcacccag ccaccacgga aacgctgtgg ttccacaaag
     3361 cgcaataaaa atacgcgcgt aatacagcca ggcgctgttc caggtatagc gtttgagcaa
     3421 aggacttagc ataaaggcca tattagtgga ttattcaaaa cgacgacgtg cattggcttc
     3481 acgcgcagct tgcgctgttt ctaccgatac cacgcgacga ccaaccggcc atagtgcaat
     3541 agcggcaatt ttaaagttcg caatgccgac aggaatgcca atgattgaaa tacattgtgc
     3601 gatgcccgtt gcaatgtgca tcaggcataa ccaccagcca aagaaaatca gccagaaaat
     3661 attcaatacc gtaccgccag tattcagcag cacatttttg ccagccgggt tcagttcatc
     3721 gacatgaata gcttcattgc cataaggcac cagagatagt ttagtgatct cccagcagga
     3781 tcgtgtcagc ggtaaggtaa aaatcagcac aatactgacc agagtcgcca acagccagcc
     3841 cagagtggtg gcaaatccgc caagcacaaa gttcagaatg ttcaaaacgg tacgcataaa
     3901 acctcgcttt actgtggttt tcagtaatgg gcggcaattg taacgttttt ttgggctgga
     3961 gcacgttttc tctgacggtt acactgataa gaaataattt cgtgtggatc tacagagtca
     4021 tggaactgaa agcgacaacg cttggaaaac gtctggcaca gcacccttac gatcgggcgg
     4081 tgatcctcaa tgccgggatt aaagtctccg gcgatcgcca cgaatacctt attcctttca
     4141 atcaattact ggcgattcac tgtaagcgcg gtctggtatg gggcgagctg gaatttgtac
     4201 tgccggacga aaaagtggtg cgtctgcacg gcaccgaatg gggcgagacg cagcgttttt
     4261 accatcatct tgatgctcac tggcggcggt ggagtggcga gatgagcgaa attgcgtctg
     4321 gtgttttacg ccagcaactg gatttgattg ccacgcgcac tggagaaaat aaatggctga
     4381 cgcgtgagca aacctctggc gttcagcaac aaatccgcca ggctttgtcg gcgttgccgt
     4441 tgccggttaa ccgactggaa gaattcgata actgccgtga ggcgtggcgt aaatgtcagg
     4501 cctggttgaa agatattgaa agcgctcggt tgcagcataa ccaggcgtat accgaagcca
     4561 tgcttaccga gtatgcggat tttttccgcc aggtcgagtc ttcaccgctg aatccggcgc
     4621 aggcccgggc agtcgttaat ggcgagcatt ctctgttagt gctggcaggt gcaggaagcg
     4681 gaaaaacgtc ggtgctggtg gcccgtgcag gctggttgct ggcgcgtggt gaagcgtccc
     4741 ctgagcaaat tttattgctg gcgtttggtc gcaaagccgc tgaagagatg gacgagcgga
     4801 ttcgcgaacg gctacatacc gaagacatta ccgcacgcac gtttcatgcg ctggcgctgc
     4861 atattattca gcagggcagc aaaaaagttc cgatagtcag caaactggaa aatgataccg
     4921 ctgcccgtca tgaactcttt attgctgagt ggcgcaagca atgcagcgaa aagaaagcgc
     4981 aggcgaaggg ctggcggcaa tggctgacgg aagaaatgca gtggtcagtg ccagaaggta
     5041 acttctggga tgatgaaaaa ttacagcgtc gccttgcctc tcgcctcgat cgctgggtaa
     5101 gtctgatgcg gatgcacggt ggtgcacagg cagaaatgat tgccagtgca cccgaagaga
     5161 ttcgcgatct gttcagtaaa cgtatcaagt tgatggcccc gttattaaaa gcctggaaag
     5221 gtgcgctgaa ggcagaaaac gctgtcgatt tttcgggcct tattcatcag gcgattgtga
     5281 ttctggagaa aggtcgcttt atcagcccgt ggaagcatat tctggttgat gaatttcagg
     5341 atatctcgcc gcagcgggca gcgttgttag cggcattacg caagcaaaac agtcagacga
     5401 cgttgttcgc tgttggtgat gactggcagg cgatttaccg attcagcggt gcgcaaatgt
     5461 cgctcaccac cgctttccat gaaaactttg gtgaaggcga acgttgtgat ttagacacga
     5521 cttaccgttt taacagtcgt atcggtaagg tggcaaaccg gtttattcag cagaacccag
     5581 gccagctgaa aaagccgcta aacagcttaa ccaatggaga caaaaaagcc gtcacgttat
     5641 tggatgagag tcaacttgac gctttgctgg ataagctctc tggttatgcc aaaccggaag
     5701 agcgcattct gatcctggcg cgttaccatc acatgaggcc tgccagcctg gaaaaagcgg
     5761 caacacgctg gccgaagttg caaatcgact ttatgaccat tcatgccagc aaagggcaac
     5821 aggcggatta cgtcatcatc gttggcttgc aggagggaag tgatggtttt ccggctgcgg
     5881 cgcgggagtc gattatggaa gaggcgctac tgccaccggt tgaggatttc ccggacgctg
     5941 aagaacggcg gttaatgtac gtggcgctga cccgggcacg ccatcgggta tgggcactgt
     6001 ttaacaaaga gaatccctct ccctttgtgg aaatactgaa aaatctggat gtgccggtgg
     6061 cgagaaaacc gtaagaaaca ggtggcgttt gccacctgtg caatattact tcagacggtc
     6121 cgcgagataa cgctgataat cggggattag aatatcgacc gcgtcgttga aatgcggcga
     6181 ctggattatg aagtctgccg ttgccacgtt ggtggcgacc ggaatgttcc ataccgtcgc
     6241 cagacgcagc aaggctttca cgtcaggatc gtgcggcacg gcatttagtg gatcccagaa
     6301 gaaaatcaat acatcaattt tcccttctga gatcaatgcg ccaacctgct ggtcaccccc
     6361 cattgggcca ctcaacatcg cgttgacgtt catgccggtc gcgcgggaaa ttaagttacc
     6421 ggtagtgcct gttgcataca gtacgtgttg ttccagtaac ggttgatgcc gttccaccca
     6481 gctcatcagc atttgtttgc agtgatcgtg tgccaccagc gcaatatgtt tccgcgcagg
     6541 taaagtgcga gtcgtcagtt ccataatgta catccgtagt taactttcct acagattact
     6601 gtaagcactt atcgctgcaa gataaagacc gaaaaagcct gcgcacaggc acaaaaatct
     6661 caggaagatg gttgtttttc cgcccactgc aggaaagtat ttcgcgtttg tgggtcagcc
     6721 agtttaaacc aatacttcag ccgttgttct gtgagcacct gagactgcgg tggaatagca
     6781 tccagctcgg aaacacccga aagcagcgta ctgtcatctg ccatcatcgt ggcaaattgc
     6841 ggtagtgaag cgcgtttcgc ggatttgtta tagcgttcaa cctcgacctc ataatcaatc
     6901 gtttttgccg ttgaggtaat ggcgagaata tccagcttta ccggaatcgg cgtcgcatcg
     6961 ccatccaaca attcaaggcg cggcgcggca tcaaaatggt tagcttcccg ctcattctcc
     7021 aggcgaggca ggcgaaaatt gacctggttg atgagctggg tattaaaact gaccaccagc
     7081 ggtggggaga tatacagccg ttcttcacta ttggaaagat gaatcgtctt ctcaacgcga
     7141 aacactaact gatgcggtcc attatccagt tcaatgctgt ccgcaccgcg cagcagagag
     7201 ctggaaacct tcttgccgtc gagtaccaga agatcgacat cggttgaaag ccgcagcgtg
     7261 gtggcaaaaa cggataccgg caaacataat gcaattaagg tggtcacgat gccggttttc
     7321 ataggtagct cctgtcaaaa gaccgcccga ataataatgt gtcaaaattt ttctttaatc
     7381 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
     7441 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
     7501 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
     7561 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
     7621 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
     7681 acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg
     7741 gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg
     7801 ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttgaa tgtggtgatg
     7861 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
     7921 aggcaaaaac cttccgggga ttcgttcagg gatcagttac gcagacgcgg ggcctggagt
     7981 tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
     8041 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
     8101 tccatcacta cgtgatacca cggagcggcg cgaagctcgt catttaccgc cagctcatca
     8161 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
     8221 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
     8281 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
     8341 caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct
     8401 gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg
     8461 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
     8521 ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc acgacacgcg
     8581 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
     8641 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
     8701 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc
     8761 taggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
     8821 ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc
     8881 aggcggctgt ctcgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc
     8941 agcagcttca tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg
     9001 aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa
     9061 atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct
     9121 gccccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
     9181 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
     9241 agtcagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata
     9301 gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg
     9361 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
     9421 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
     9481 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
     9541 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
     9601 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc
     9661 gtaggatttc gctacaccac acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc
     9721 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa
     9781 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc
     9841 agcgagccgc atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt
     9901 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
     9961 cggtataaat agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc
    10021 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
    10081 acttcccagt gttccggtga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc
    10141 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
    10201 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
    10261 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg
    10321 gcgaagccca gaatgctcag caggttgacg aagatgttgt acagcgaaac atacaggcta
    10381 accgtggcac gaatatagtt cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa
    10441 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagc
    10501 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
    10561 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac
    10621 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
    10681 gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
    10741 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
    10801 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
    10861 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
    10921 ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
    10981 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
    11041 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc
    11101 aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca tttagctaac
    11161 cccttatctt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
    11221 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
    11281 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
    11341 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
    11401 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
    11461 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa aa
//
LOCUS       DAZRUF010000129        11434 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZRUF010000129 DAZRUF010000000
VERSION     DAZRUF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.129"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            380..1948
                     /gene="cydA"
                     /locus_tag="ACHXOL_002732"
     CDS             380..1948
                     /gene="cydA"
                     /locus_tag="ACHXOL_002732"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR6809673.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1964..3103
                     /gene="cydB"
                     /locus_tag="ACHXOL_002733"
     CDS             1964..3103
                     /gene="cydB"
                     /locus_tag="ACHXOL_002733"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR6809674.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3118..3231
                     /gene="cydX"
                     /locus_tag="ACHXOL_002734"
     CDS             3118..3231
                     /gene="cydX"
                     /locus_tag="ACHXOL_002734"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR6809675.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3231..3524
                     /gene="ybgE"
                     /locus_tag="ACHXOL_002735"
     CDS             3231..3524
                     /gene="ybgE"
                     /locus_tag="ACHXOL_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR6809676.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3674..4078
                     /gene="ybgC"
                     /locus_tag="ACHXOL_002736"
     CDS             3674..4078
                     /gene="ybgC"
                     /locus_tag="ACHXOL_002736"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR6809677.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4075..4767
                     /gene="tolQ"
                     /locus_tag="ACHXOL_002737"
     CDS             4075..4767
                     /gene="tolQ"
                     /locus_tag="ACHXOL_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR6809678.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4771..5199
                     /gene="tolR"
                     /locus_tag="ACHXOL_002738"
     CDS             4771..5199
                     /gene="tolR"
                     /locus_tag="ACHXOL_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR6809679.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5264..6496
                     /gene="tolA"
                     /locus_tag="ACHXOL_002739"
     CDS             5264..6496
                     /gene="tolA"
                     /locus_tag="ACHXOL_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR6809680.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            6629..7921
                     /gene="tolB"
                     /locus_tag="ACHXOL_002740"
     CDS             6629..7921
                     /gene="tolB"
                     /locus_tag="ACHXOL_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR6809681.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7956..8477
                     /gene="pal"
                     /locus_tag="ACHXOL_002741"
     CDS             7956..8477
                     /gene="pal"
                     /locus_tag="ACHXOL_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR6809682.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8487..9278
                     /gene="cpoB"
                     /locus_tag="ACHXOL_002742"
     CDS             8487..9278
                     /gene="cpoB"
                     /locus_tag="ACHXOL_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR6809683.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9443..9518
                     /locus_tag="ACHXOL_002743"
     tRNA            9443..9518
                     /locus_tag="ACHXOL_002743"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9476..9478,aa:Lys,seq:ttt)
     gene            9668..9743
                     /locus_tag="ACHXOL_002744"
     tRNA            9668..9743
                     /locus_tag="ACHXOL_002744"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9701..9703,aa:Val,seq:tac)
     gene            9747..9822
                     /locus_tag="ACHXOL_002745"
     tRNA            9747..9822
                     /locus_tag="ACHXOL_002745"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9780..9782,aa:Lys,seq:ttt)
     gene            9969..10044
                     /locus_tag="ACHXOL_002746"
     tRNA            9969..10044
                     /locus_tag="ACHXOL_002746"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10002..10004,aa:Lys,seq:ttt)
     gene            10078..10153
                     /locus_tag="ACHXOL_002747"
     tRNA            10078..10153
                     /locus_tag="ACHXOL_002747"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10111..10113,aa:Lys,seq:ttt)
     gene            10178..10309
                     /locus_tag="ACHXOL_002748"
     ncRNA           10178..10309
                     /ncRNA_class="other"
                     /locus_tag="ACHXOL_002748"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10558..>11434
                     /gene="nadA"
                     /locus_tag="ACHXOL_002749"
     CDS             10558..>11434
                     /gene="nadA"
                     /locus_tag="ACHXOL_002749"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR6809684.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT         2834 a         2739 c         3059 g         2802 t
ORIGIN      
        1 attgaaacat gattaacata atttgtagga attgatattt atcaatgtat aagtcttgga
       61 aattggcatc aaaaagagat aaattgttct cgatcaaatt ggctgaaagg cggtaattta
      121 gctataaatt gatcaccgtc gaaaaatgca aatttgcttc aacaaaaacc tgtttattgt
      181 aaggattttg cggcgtaata tatacgtggg atcaatttga gtttttatta acatgtttgc
      241 aacctttctt tacgccgttt ttgtgtgcat tcacatggta tgatgaaagt gttcaaacaa
      301 atttctattg gggcatgcgt gtgacccttt ctaacggggt tcactctcgg agtcttcatg
      361 cgatgagcaa ggagtcatga tgttagatat agtcgaactg tcgcgcttac agtttgcctt
      421 gaccgcgatg taccacttcc tttttgtgcc actgacgctc ggtatggcgt tcctgctggc
      481 cattatggaa acggtctacg tcctctccgg caaacagatt tataaagata tgaccaagtt
      541 ctggggcaag ttgtttggta tcaacttcgc tctgggtgtg gctaccggtc tgaccatgga
      601 gttccagttc gggactaact ggtcttacta ttcccactat gtaggggata tcttcggtgc
      661 gccgctggca atcgaaggtc tgatggcctt cttcctcgaa tccacctttg taggtctgtt
      721 cttcttcggt tgggatcgtc tgggtaaagt tcagcatatg tgtgtcacct ggctggtggc
      781 gctcggttca aacctgtccg cactgtggat tctggttgcg aacggctgga tgcaaaaccc
      841 aatcgcgtcc gatttcaact ttgaaactat gcgtatggag atggtgagct tctccgagct
      901 ggtgcttaac ccggttgctc aggtgaaatt cgttcacact gtagcgtctg gttatgtgac
      961 tggcgcgatg ttcatcctcg gtatcagcgc atggtatatg ctgaaaggtc gtgacttcgc
     1021 cttcgctaaa cgctcctttg ctatcgctgc cagcttcggt atggctgctg ttctgtctgt
     1081 tattgttctg ggtgatgaat ccggctacga aatgggcgac gtgcagaaaa ccaaactggc
     1141 tgctattgaa gccgagtggg aaacgcaacc tgcgcctgct gcctttactc tgttcggcat
     1201 tcctgatcag gaagaggaga cgaacaaatt tgcgattcag atcccttacg cactgggcat
     1261 cattgcaacg cgttccgtgg ataccccggt tatcggcctg aaagagctga tggtgcagca
     1321 tgaagaacgc attcgtaacg ggatgaaggc gtactctctg ctcgaacaac tgcgttctgg
     1381 ttctaccgac caggcggttc gtgaccagtt caatagcatg aagaaagacc tcggttacgg
     1441 tctgctgctg aaacgctata cgccaaacgt ggctgatgcg actgaagcgc agattcaaca
     1501 ggcaaccaaa gactccatcc cgcgtgtagc gccgctgtac tttgcgttcc gtatcatggt
     1561 ggcgtgtggc ttcctgcttc tggcaatcat cgcgctctct ttctggagtg tcatccgcaa
     1621 ccgcattggc gagaaaaaat ggcttctgcg cgccgcgctg tacggtattc cgctgccgtg
     1681 gattgctgta gaagcgggct ggttcgtggc tgaatatggc cgccaaccgt gggctatcgg
     1741 tgaagtgctg ccgacagctg tggcgaactc gtcactgacc gcaggcgatc tcatcttctc
     1801 aatggtgctg atttgcggcc tgtataccct gttcctggtg gcagaattgt tcttaatgtt
     1861 caagtttgca cgcctcggcc caagcagcct gaaaaccggt cgctatcact ttgagcagtc
     1921 ttccacgact actcagccgg cacgctaaga caggagtcgt caaatgatcg attatgaagt
     1981 attgcgtttt atctggtggc tgctggttgg cgttctgctg attggttttg cagtcactga
     2041 cggtttcgac atgggggtgg gcatgctcac ccgtttcctc ggtcgtaacg acaccgagcg
     2101 tcgaattatg attaactcca ttgcaccaca ctgggacggt aaccaggttt ggctgatcac
     2161 cgcgggcggc gcactctttg ctgcctggcc gatggtctat gccgctgcgt tctccggctt
     2221 ctatgtggcg atgatcctcg tgctggcgtc tttgttcttc cgtccggtcg gttttgacta
     2281 ccgctccaag attgaagaaa cccgctggcg taacatgtgg gactggggca tcttcattgg
     2341 tagtttcgtt ccgccgctgg taattggtgt agcgttcggt aacctgttgc agggcgtacc
     2401 gttcaacgtt gatgaatatc tgcgtctgta ctacaccggt aacttcttcc agttgcttaa
     2461 cccgttcggc ctgctggcag gcgtggtgag cgtagggatg atcattactc agggcgcaac
     2521 ctatctgcaa atgcgtaccg tgggcgaact gcacctgcgt acccgtgcaa cggctcaggt
     2581 ggctgcgctg gtgacactgg tctgtttcgc actggctggc gtatgggtga tgtacggtat
     2641 cgatggttat gtcgtgaaat cgacaatgga ccattacgca gcctctaacc cactgaataa
     2701 agaagtggtt cgtgaagctg gcgcatggct ggttaacttc aacaacacgc caattctgtg
     2761 ggctattccg gcactgggtg tggttcttcc gctgctgacc atcctgactg cacgtatgga
     2821 taaagccgcg tgggcgtttg tgttctcctc cctgacgctg gcctgcatca tcctgacagc
     2881 cggtatcgca atgttcccgt ttgtgatgcc gtccagcacc atgatgaacg caagtctgac
     2941 aatgtgggat gcaacttcca gccagctgac gcttaacgtc atgacttggg ttgcggtggt
     3001 tctggtaccg atcattctgc tctacaccgc ttggtgttac tggaaaatgt tcggtcgtat
     3061 caccaaagaa gatattgaac gtaacaccca ctctctgtac taagtaagga gctaaaaatg
     3121 tggtatttcg catggattct gggaacgctt cttgcctgtt cgtttggggt aatcaccgcg
     3181 ctggcgcttg aacacgtcga atcaggcaaa gccggtcaag aagacatctg atgagtaaga
     3241 ttatcgcgac cttgtatgcg gtaatggaca agcgccccct gcgggcgctt tccttcgtga
     3301 tggcgcttct gttagcagga tgtatgtttt gggacccatc tcgttttgcc gcgaagacca
     3361 gtgaactgga aatctggcat ggtttattgc tgatgtgggc cgtctgtgct ggtgtgattc
     3421 acggcgtggg ctttcgtccg cagaaggttc tttggcaagg gattttttgc ccattgcttg
     3481 ccgatattgt tctcattgtc gggctgattt tcttcttctt ttaaatcaga attctcttta
     3541 aaaaattatg ggccgctcca ggcccataaa tttttacgct cccttaactt gccctcattc
     3601 ccaaacctca atcgcgcgcg tatagtagca gcgtttaaaa gctctaactt ttgttgcatt
     3661 accgggatgt aaagtgaata caacgctgtt tcgatggccg gttcgcgtct actatgaaga
     3721 taccgatgcc ggtggtgtgg tgtaccacgc cagttacgtc gctttttatg aaagagcacg
     3781 cacagagatg ctgcgtcatc atcacttcag tcagcaggcg ctgatggctg aacgcgttgc
     3841 ctttgtggta cgtaaaatga cggtggaata ttacgcacct gcgcggctcg acgatatgct
     3901 cgaaatacag actgaaataa catcaatgcg tggcacctct ttggttttca cgcaacgtat
     3961 tgtcaacgcc gagaatactt tgctgaatga agcagaggtt ctggttgttt gcgttgaccc
     4021 actcaaaatg aagcctcgtg cgcttcccaa gtctattgtc gcggagttta agcagtgact
     4081 gacatgaata tccttgattt gttcctgaag gctagccttc tggttaaact tatcatgttg
     4141 attttgattg gtttttcaat cgcatcctgg gccattatta tccagcggac ccgtattctt
     4201 aacgcagcgg cgcgcgaagc cgaagcgttt gaagataaat tctggtctgg aatcgaactc
     4261 tctcgcctct atcaagagag tcaggggaaa cgggataatc tgactggttc ggaacaaata
     4321 ttttacagcg ggttcaaaga gtttgtgcgc ctgcatcgtg ccaatagcca tgcgccggaa
     4381 gccgtagtgg aaggggcgtc gcgtgctatg cgtatctcca tgaaccgtga acttgaaaat
     4441 ctggaaacgc acattccgtt cctcggtacg gttggctcca tcagcccgta tattggtctg
     4501 tttggtacgg tctgggggat catgcacgcc tttatcgccc tcggggcggt aaaacaagca
     4561 acactgcaaa tggttgcgcc cggtatcgca gaagcgttga ttgcgactgc aattggtctg
     4621 ttcgccgcaa ttccggcggt aatggcttat aaccgcctca accagcgcgt aaacaaactg
     4681 gaactgaatt acgacaactt tatggaagag tttaccgcga ttctgcaccg ccaggcgttt
     4741 accgttagcg agagcaacaa ggggtaagcc atggccagag cgcgtggacg aggtcgtcgc
     4801 gatctcaagt ccgaaatcaa cattgtaccg ttgctggacg tactgctggt gctgttgctg
     4861 atctttatgg cgacagcgcc catcatcacc cagagcgtgg aggtcgatct gccagacgct
     4921 actgaatcac aggcggtgag cagtaacgat aatccgccag tgattgttga agtgtctggt
     4981 attggtcagt acaccgtggt ggttgagaaa gatcgcctgg agcgtttacc accagagcag
     5041 gtggtggcgg aagtgtccag ccgtttcaag gccaacccga aaacggtctt tctgatcggt
     5101 ggcgcaaaag atgtgcctta cgatgaaata attaaagcac tgaacttgtt acatagtgcg
     5161 ggtgtgaaat cggttggttt aatgacgcag cctatctaaa catctgcgtt tcccttgctt
     5221 gaaagagagc gggtaacagg cgaacagttt ttggaaaccg agagtgtcaa aggcaaccga
     5281 acaaaacgac aagctcaagc gggcgataat tatttcagca gtgctgcatg tcatcttatt
     5341 tgcggcgctg ctctggagtt cgttcgatga gaatatagaa gcttcagccg gaggcggcgg
     5401 tggttcgtcc atcgacgctg tcatggttga ttcaggtgcg gtagttgagc agtacaaacg
     5461 catgcaaagc caggaatcaa gcgcgaagcg ttctgatgag cagcgcaaga taaaggaaca
     5521 gcaggctgct gaagaactgc gtgagaaaca agcggctgaa caggaacgcc tgaagcaact
     5581 tgagaaagag cggttagctg ctcaggaaca gaaaaagcag gctgaagaag ccgcaaaaca
     5641 ggccgagtta aagcagaagc aagcggaaga ggcggcagcg aaagcggcgg cagatgctaa
     5701 agcgaaggcc gaagcggatg ctaaagctgc ggaagaagca gcgaagaaag cggcggcaga
     5761 cgcgaagaaa aaagcagaag cagaagccgc caaagccgca gccgaagcgc agaaaaaagc
     5821 cgaggcagcc gctgcggcac tgaagaagaa agcggaagcg gcagaagctg ctgcagctga
     5881 agcaagaaag aaagcggcag cagagaaagc tgcagccgac aaaaaagcag cagaaaaagc
     5941 ggctgctgat aaggcagcag ctgataagaa agcagcggca gaaaaagccg ccgcagacaa
     6001 aaaagcggca gcggcaaaag cagcggcaga aaaagccgcc gcagacaaaa aagcggcagc
     6061 ggcaaaagca gcagctgaaa aagccgctgc agcaaaagct gccgcagagg cagatgatat
     6121 tttcggtgag ctaagctctg gtaagaatgc accgaaaacg gggggagggg cgaaagggaa
     6181 caatgcttcg cctgccggga gtggtaatac taaaaacaat ggcgcatcag gggccgatat
     6241 caataactat gccgggcaga ttaaatctgc tatcgaaagt aagttctatg acgcatcgtc
     6301 ctatgcaggc aaaacctgta cgctgcgcat aaaactggca cccgatggta tgttactgga
     6361 tatcaaacct gaaggtggcg atcccgcact ttgtcaggct gcgttggcag cagctaaact
     6421 tgcgaagatc ccgaaaccac caagccaggc agtatatgaa gtgttcaaaa acgcgccatt
     6481 ggacttcaaa ccgtaatcgc gatgttgact gttccgacgg tcaacatcag gcaccggttg
     6541 ccacggggtt ctggtagttt tgtgtatttt agtttgttaa cattctgcta aattatcgtg
     6601 ggccatcggt ccagataagg gagatatgat gaagcaggca ttacgagtag catttggttt
     6661 tctcatactg tgggcatcag ttctgcatgc tgaagtccgc attgtgatcg acagcggtgt
     6721 agattccggt cgtcctattg gtgttgttcc tttccagtgg gcgggccctg gtgcggcacc
     6781 tgaagatatt ggcggcatcg ttgctgccga cttgcgtaac agcggtaaat ttaatccgtt
     6841 agatcgcgct cgtctgccac agcagccggg tagtgcgcag gaagtacaac cagctgcatg
     6901 gtccgcgctg ggtattgacg ctgtagttgt cggtcaggtc actccgaatc cggatggttc
     6961 ttacaatgtt gcttatcaac ttgttgacac tggcggcgca ccgggtactg tacttgctca
     7021 gaactcgtac aaagtgaaca agcagtggct gcgctatgct ggtcataccg ccagtgatga
     7081 agtgtttgaa aaactgaccg gcattaaagg tgcgttccgt acccgtattg cctacgttgt
     7141 tcagaccaac ggcggtcagt tcccgtatga actgcgcgta tctgactatg acggttacaa
     7201 ccagtttgtc gttcaccgtt caccacagcc gctgatgtcc ccggcgtggt caccagacgg
     7261 ttctaaactg gcttatgtga ccttcgaaag cggtcgttcc gcgctagtta tccaaacgct
     7321 ggcaaacggc gctgtacgtc aggtggcttc attcccgcgt cacaacggtg cgcctgcatt
     7381 ctcgccagac ggcagcaaac tggcattcgc cttgtcgaaa accggtagcc tgaacctgta
     7441 cgtaatggat ttggcttctg gtcagatccg ccaggtgact gatggtcgca gtaacaatac
     7501 cgaaccgacc tggttcccgg acagccagaa cctggcattt acttctgacc aggccggtcg
     7561 tccacaggtt tataaagtga atatcaacgg cggtgcgcca caacgtatta cctgggaagg
     7621 ttcgcagaac caggatgcgg atgtcagcag cgacggtaaa tttatggtaa tggtcagctc
     7681 caacggtggg cagcagcaca ttgccaaaca agatctggca acgggaggcg tacaagttct
     7741 gtcgtccacg ttcctggatg aaacgccaag tctggcacct aacggcacta tggtaatcta
     7801 cagctcttct caggggatgg gatccgtgct gaatttggtt tctacagatg ggcgtttcaa
     7861 agcgcgtctt ccggcaactg atggacaggt caaattccct gcctggtcgc cgtatctgtg
     7921 ataataatta attgaatagt aaaggaatca ttgaaatgca actgaacaaa gtgctgaaag
     7981 ggctgatgat tgctctgcct gttatggcaa ttgcggcatg ttcttccaac aagaacgcca
     8041 gcaatgacgg cagcgaaggc atgctgggtg ccggcactgg tatggatgcg aacggcggca
     8101 acggcaacat gtcttccgaa gagcaggctc gtctgcaaat gcaacagctg cagcagaaca
     8161 acatcgttta cttcgatctg gacaagtacg atatccgttc tgacttcgct caaatgctgg
     8221 atgcacatgc aaacttcctg cgtagcaacc cgtcttacaa agtcaccgta gaaggtcacg
     8281 cggacgaacg tggtactccg gaatacaaca tctccctggg tgaacgtcgt gcgaacgccg
     8341 ttaagatgta cctgcagggt aaaggcgttt ctgcagacca gatctccatc gtttcttacg
     8401 gtaaagaaaa acctgcagta ctgggtcatg acgaagcggc atactccaaa aaccgtcgtg
     8461 cggtactggt ttactaagag aattgcatga gcagtaactt cagacatcaa ctattgagtc
     8521 tgtcgttact ggttggtata gcggccccct gggccgcttt tgctcaggca ccaatcagta
     8581 gtgtcggctc aggctcggtc gaagaccgcg tcactcaact tgagcgtatt tctaatgctc
     8641 acagccagct tttaacccaa ctccagcaac aactttctga taatcaatcc gatattgatt
     8701 ccctgcgtgg tcagattcag gaaaatcagt atcaactgaa tcaggtcgtg gagcggcaaa
     8761 agcagatcct gttgcagatc gacagcctca gcagcggtgg tgcagcggcg caatcaacca
     8821 gcagcgatca aagcggtgcg gcggcatcaa cgacgccgac agctgatgct ggtactgcga
     8881 atgctggcgc gccggtgaaa agcggcgatg caaacacgga ttacaatgca gctattgcgc
     8941 tggtgcagga taaatcccgc caggatgacg caatggtggc atttcagaat ttcatcaaaa
     9001 attaccctga ttcaacttac ctgccaaacg ccaattattg gctgggtcag ttaaactaca
     9061 acaagggtaa aaaagatgat gcggcgtact attttgcttc ggtagtgaaa aactatccga
     9121 agtcaccaaa ggctgctgat gcgatgttta aagtcggcgt catcatgcag gacaaaggtg
     9181 acaccgcaaa agcgaaagcc gtataccagc aggttatcag taaataccct ggtactgatg
     9241 gcgctaaaca ggcacaaaaa cgtctgaacg cgatgtaatg cataacacac gaccagaagt
     9301 cgcattattt ctggtcgtgt cgtgcgaatc ataagcagtt gagtgatctg catcgaaatt
     9361 tttgttgcgc tcaagtctga aatcagtaat atatgccgcc gttgccacgg gatatcaaac
     9421 aaaccgaaag caacgaaaaa gtgggtcgtt agctcagttg gtagagcagt tgacttttaa
     9481 tcaattggtc gcaggttcga atcctgcacg acccaccagt tttagcatcg aagacagatg
     9541 ttaagcgtgt aggataacgt tgcgtcagca acggcccgta gggcgagcga agcgagtcat
     9601 cctgcacgac ccaccactaa ttacggtggg ttcggtggaa gtagtttgta gtatccagcg
     9661 cagtatcggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
     9721 ttcgatcccg tcatcaccca ccactcgggt cgttagctca gttggtagag cagttgactt
     9781 ttaatcaatt ggtcgcaggt tcgaatcctg cacgacccac caattttaac atcaaactca
     9841 gatgttaagc gtgaaggata acgttgcgcc agcaacggcc cgtagggcaa gcgaagcgag
     9901 tcatcctgca cgacccacca atcttaaaga ttggccccga gtagaaatct ttcaggtaac
     9961 acccgtatgg gtcgttagct cagttggtag agcagttgac ttttaatcaa ttggtcgcag
    10021 gttcgaatcc tgcacgaccc accatcctga atgattaaag cagcataatc ccgcaagggg
    10081 tcgttagctc agttggtaga gcagttgact tttaatcaat tggtcgcagg ttcgaatcct
    10141 gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt ttttgctatc tgagatattc
    10201 aaagattcga acctgcagca ggttcgagtt gagcgcagcg aaacaacgga gccgctcgcg
    10261 gcgacggccc gaagggcgag cgaagcgagt catcctgcac gacccaccaa tgtaaaaaag
    10321 cgccctaaag gcgctttttt gctattcagg catcctcaat ttcactttgt aaacctgatg
    10381 acatcgtcag agcttactgt gcaagcaact ctacgtcggt ggaattaggc gtaattcgag
    10441 tgacttttcc ccaccattcg actatcttgt ttagcatata aaacaaatta cgccgatagt
    10501 agcgaatatt acgctaatgt cggttttaac gttaagcctg taaaacgaga tggtaagatg
    10561 agcgtgatgt ttgatccaga cacggcgatt tatcctttcc ccccgaagcc gacgccgtta
    10621 agcattgatg aaaaagcgta ttaccgcgag aagataaaac gtctgctaaa agaacgtaat
    10681 gcggtgatgg ttgcccacta ctataccgac cccgaaattc aacaactggc agaagaaacc
    10741 ggtggctgta tttctgattc tctggaaatg gcgcgcttcg gtgcaaagca tcccgcttct
    10801 acgttgttag tcgctggggt gagatttatg ggagaaaccg ccaaaattct cagtccggaa
    10861 aaaacaattc tgatgctgac acttcaggct gaatgttcac tggatctcgg ctgccctgtt
    10921 gaagaattta acgcattttg cgatgcccat cccgatcata ctgtcgtcgt ctacgccaac
    10981 acttctgctg cggtaaaagc gcgcgcagat tgggtggtaa cttcaagcat tgccgtcgaa
    11041 cttattgatc atctggatag ttttggtgaa aaaatcatct gggcacccga caaacatctg
    11101 gggcgttacg tgcaaaaaca gacgggcgca gacattttat gctggcaggg tgcctgtatt
    11161 gtgcatgatg aatttaagac tcaggcgtta acccgcttgc aagaagaata cccggatgct
    11221 gccatactgg tgcatccaga atcaccacaa gctattgtcg atatggcgga tgcggtcggt
    11281 tccaccagtc aactgatcgc tgctgcgaaa acattgccac atcagcggct tatcgtggca
    11341 accgatcggg gcattttcta caaaatgcag caggcggtgc cagataaaga gttactggaa
    11401 gcaccaaccg caggtgaggg tgcaacctgc cgca
//
LOCUS       DAZRUF010000130        11428 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZRUF010000130 DAZRUF010000000
VERSION     DAZRUF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.130"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHXOL_002750"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHXOL_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR6809685.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHXOL_002751"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHXOL_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR6809686.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHXOL_002752"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHXOL_002752"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR6809687.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHXOL_002753"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHXOL_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR6809688.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHXOL_002754"
     CDS             complement(3928..5214)
                     /locus_tag="ACHXOL_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR6809689.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHXOL_002755"
     CDS             complement(5235..6026)
                     /locus_tag="ACHXOL_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR6809690.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHXOL_002756"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHXOL_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR6809691.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHXOL_002757"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHXOL_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR6809692.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHXOL_002758"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHXOL_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR6809693.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHXOL_002759"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHXOL_002759"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR6809694.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHXOL_002760"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHXOL_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR6809695.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHXOL_002761"
     CDS             complement(9882..10364)
                     /locus_tag="ACHXOL_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR6809696.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHXOL_002762"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHXOL_002762"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR6809697.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2804 a         2748 c         3060 g         2816 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccaa ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat tatcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggcggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctacc aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAZRUF010000131        11311 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZRUF010000131 DAZRUF010000000
VERSION     DAZRUF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.131"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..494
                     /locus_tag="ACHXOL_002763"
     CDS             <1..494
                     /locus_tag="ACHXOL_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR6809698.1"
                     /translation="VFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTDNTA
                     LPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPLTVT
                     WGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSIYLS
                     QDI"
     gene            586..3270
                     /locus_tag="ACHXOL_002764"
     CDS             586..3270
                     /locus_tag="ACHXOL_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR6809699.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            3267..4355
                     /locus_tag="ACHXOL_002765"
     CDS             3267..4355
                     /locus_tag="ACHXOL_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR6809700.1"
                     /translation="MRNRLIAAILGLFGTLIGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4438..5735)
                     /gene="exuT"
                     /locus_tag="ACHXOL_002766"
                     /pseudo
     CDS             complement(4438..5735)
                     /gene="exuT"
                     /locus_tag="ACHXOL_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            6218..7630
                     /gene="uxaC"
                     /locus_tag="ACHXOL_002767"
     CDS             6218..7630
                     /gene="uxaC"
                     /locus_tag="ACHXOL_002767"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR6809701.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7645..9132
                     /gene="uxaA"
                     /locus_tag="ACHXOL_002768"
     CDS             7645..9132
                     /gene="uxaA"
                     /locus_tag="ACHXOL_002768"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR6809702.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9215..9766
                     /locus_tag="ACHXOL_002769"
     CDS             9215..9766
                     /locus_tag="ACHXOL_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR6809703.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9771..11015)
                     /gene="sstT"
                     /locus_tag="ACHXOL_002770"
     CDS             complement(9771..11015)
                     /gene="sstT"
                     /locus_tag="ACHXOL_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR6809704.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2995 a         2738 c         2927 g         2651 t
ORIGIN      
        1 aggtatttgc aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg
       61 cagtacaaaa ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga
      121 ccgataacac cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc
      181 aatcttacca gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc
      241 agttagtttc cccggcgcaa ctggttcaaa gcctggatgc tagcaagatt gttccgctga
      301 cagtaacctg gggcggtgaa gaaattaaag ctgatgcggc aaccactttt accgcaacca
      361 aaatctttgc ttccgatgcg ttaactaacg gttctctggc taaaaacctg atgtttgctc
      421 aaaccaccaa aggtgttctg gagacaggca tctaccgtgg tgtagtaagc atttatctgt
      481 ctcaggatat ctaatccaga taacagaatt aacacgtaac aaagggaggg gtagccctcc
      541 cttattttaa ctgttttagt atttgttatg gatttcagga ttgccatgga taaaaaatta
      601 ctggctcttt tgatcctggc gagtctcagc ccggcagagg cgacattaac cgaaattccc
      661 gcagggtttg aggttattgc tcagggacag caggagtata tcgaggttta tttttcaggg
      721 aaaagtctcg gtaaatatta tgcaatggtt aatcttgata ccgtaacatt tcttgatcca
      781 gcaagtctat ataacaagct ggaactggat gtagacgatc agaaaatcgc gcatatagtg
      841 aaagaaaaat tatcgcagcc gctagctcgc cacggtgaat tggcttgcgg ttatgtacgt
      901 actgactcag ggtgtggttt tctgaatacc gatacgctgg aaataatcta taatgatgaa
      961 gaaagttcgg caacgttgtt tattaatccg caatggaatt cagctttcga tgcgaagtca
     1021 ttatatttaa atccagacaa aaatactgtt aatgctttta tacatcagca agacatcaat
     1081 gttctggcac aggatgatta ccaatcgttg tctattcagg gaaacggtgc gctgggaata
     1141 acagaaaata gttatattgg tgcacactgg aatttcaacg gttatgatgc agatgatgtc
     1201 agtgacagta atgctgacgt cagcgatctc tattatcgtt atgatttttt acgtcgttat
     1261 tatgtgcagg cgggtcgcat ggacaaccgc acactattta atgcacaagg cgggaacttt
     1321 acctttaact ttctgccact cggtgcaatt gatgggatgc gtatcgggtc gacactgagc
     1381 tatttaaacc aggcgcaaag ccagcaagga accccagtaa tggttctgct ttcgcgcaat
     1441 tctcgtgttg acgcttatcg taatgagcaa cttttgggat cgttttatct caatagtggt
     1501 tcgcaattta ttgataccag ttcctttccg ccaggcagct acagcgtggc attaaaagtc
     1561 tacgaaaata accaactcac ccgcaccgag cttgtgccgt ttaccaaaac cggcggtctg
     1621 actgacggaa atgcgcaatg gttcttacag gcaggtaaaa ctacatcaca ggtttctgat
     1681 gatgaaagtt cagcttatca gctaggagta cgcctgccat tacatccgca atatgagctc
     1741 tacgcagggc tggcgaatgc cgatgatgtg agtgctttcg agttaggtaa taactggacg
     1801 gcagatttag gcggggtggg gaatcttgca atcagcgcca gcgtgttccg taacgatgac
     1861 ggcggcaaag gtgatatgca acaggccaac tggagtaatc cgggatggcc gacgttgggc
     1921 ttttatcgga ccaactctga cggcgatgct tgtacaaccg acagcagaga gagctataac
     1981 gccttaagct gttatgaaag tatttccgcg acggtttcac agaattttgt cggctggaat
     2041 atgatgctgg gttatacccg cacacaaaat aacactgatg atagtttgcg ttgggataaa
     2101 cagcagagct ttgaaaataa ctatcttcgc cagacaacag cgcaaagtat ctctgaaact
     2161 gtacaactta gcgcttcccg cgcttttgtg atgcgtgact ggattttgag tacgtcagtc
     2221 ggtgttttcc atcgtaatga caacggtggc gataacgacg acaacggctt gtacttatcg
     2281 ttttcgttat ctgacacgcc aacgatggat agcaataaca acagccattc aacaaatgtg
     2341 tctacggatt atcgttatag cgaacaggat ggcgatcaaa cgtcatggca gttatcgcat
     2401 accttttata acgattcatt cagccataaa gaacttggcg taaccgtcgg aggcctgaac
     2461 accgatacca taaacagcgc ggttaacggg cgttgggatg ggcaatacgg aaatgtctac
     2521 gctaccgtat ctgatagtta tgaccgtaag aatcatgatc atctttcggc ctttacgggc
     2581 acttatagct ctacgctggc tgtcagttgc tatggcgtta atttgggagc cagtggtaca
     2641 gacgatttgc tgggtgcggt actggtggat gtgaaaggct tctctgaaca ggatgaagag
     2701 agtcaggatc tgcaactcga agcgcgggtg gcaggcagcc gaactttgca gcttggtcaa
     2761 agtgacagtg tgttgttccc ttatcctgga tttcagtctg gttttgttga ggttaacgac
     2821 agtagccagg gcaatcagca ggggacaaca aacatcatta acggtgcagg gaatcgtgaa
     2881 ttaatgttgt tgcctggcaa gctgcgctat cgcgaagtgt ctgccagctt taactacaac
     2941 tatatcggtc gtttattact gccggcagcg gtgaaaaaat tcccgattgt ggggctgaac
     3001 agcgccatgt tactggtagc tgaagatgga ggatttacac ttgaaattaa cggcagcgaa
     3061 aaagagctct atctgctttc cgggcagcaa ttccttaagt gtccactgag tgttgtaaag
     3121 aaacgcgcca gcattcgtta cagcggagat gttacttgta gtgtggtgac ttattcacaa
     3181 ttaccggagt cgattcaggt tcaggcacag ttgaaacagc ctaaattacg tggaaacgtt
     3241 cagacggcgc aaagggaggt tgcaccatga gaaatcgatt gattgcggcg atattgggct
     3301 tgtttggcac gctcattggc gttcaggcag ctcctgacgt gaccagtgaa attacgtatg
     3361 atttggcatc tggtagagcg gattattact tctggaagga tgaagcgtcg gcaggaaata
     3421 atggatatat gtggtatgaa tgttcgtatc ctgacctcca acaaacctgt acagctaatg
     3481 gaaatatatc gacagtacaa atctatttaa ctgaacaacg cagtgggatg cgttggccgg
     3541 taaaactcaa aggatttaaa acagccattg taagtagcga tgaagcgccg ccaggatgca
     3601 aggggggcaa agggcttcag acgaatctta aggattctaa tagatcttca tgtacagaag
     3661 atggtcaaca ttattatata tacgatacaa agtttcttac gctctacctt gagcagacag
     3721 agatgaagaa tttgccgatt ggtggcgtct ggaaggggaa agttaaatta cattcgaaca
     3781 gcccggccca ggactatttc gcaaatatta ccctgaatac gctcgacccc aaccatattg
     3841 acgtgttctt cccggagttc gcccacgcca cgccaagggt gcagttagac ttgcatccaa
     3901 caggaagcgt taacggcagc aactacgcgc aagatctgac catgttggac atgtgcctgt
     3961 acgatggttt taacggtaat gccatcagtt atgaaatcat gctcaaagat gaagggcgac
     4021 cagctgcagg gcgcagagac ggtgacttct ctatctatcg tcaaggaggg accaccaccg
     4081 acgagggaga acgcattgat taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa
     4141 ttgatgtgcg caataatgaa aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc
     4201 cggtggtact gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg
     4261 cagtcgataa attcagcgtg atggataaac aggccgggta ctacatgggc aaattgtcag
     4321 taatctttac gccttccttg ccaaccatca attgaccccg cagaccagaa aaaactaaac
     4381 aacgaagccg cctgataagg cggcttcgtc attacaactt acgttaaacg ttaaacgtta
     4441 atgttgcggt gcgggatcgt tatgtgtttc ctgcgccacc tcaatcgccg gtttgttctg
     4501 caatacggtc cagataacca gcgcacccag caggtcaaat actgccagaa ctgcgaacag
     4561 cgggctgaag ccgatggtgt cagccagtgc accgacaacc agcgcaaaca gcgtacttgc
     4621 cagccatgcg gacatcccgg ttaaaccgtt cgccgtcgcc acttcgttac gaccgaacac
     4681 gtcagaagag agcgtaatca gcgcgccaga cagtgcctgg tgggcaaaac caccgataca
     4741 cagcagcata attgcgacat acgggttggt gaacagaccg atcatacccg ggccaatcat
     4801 cagcacagca cccagcgtta cgaccatctt acgggaaacg atcaggttca caccaaacca
     4861 acgctggaac agcggcggca ggtaaccacc gaggatacaa ccgaggtcag caaacagcat
     4921 cggcatccag gcgaacatcg cgatctcttt caggttaaag ccgtaaactt taaacatgaa
     4981 cagcgggatc cacgcgttaa aagtacccca ggccggttct gccaggaaac gcggcagtgc
     5041 gataccccag aactgacggt tacgcaggat ctgaccaacg gacattttct tcgccgtgct
     5101 cacctggtgc tgggcttcct gaccattaat aatatagtcg cgttcttcat cggtcagatg
     5161 cttctggtcg cgcggatgtt tatagaaaat cagccatgcc atcgcccaga taaagctcaa
     5221 tgcaccggag atgataaatg ccatctgcca gctgtgcatc acgattgcca taccaccagc
     5281 ggcggcgcaa tcatcgcacc aatcgaagaa tctacgttaa agtagcctac tgcgatggaa
     5341 cgctctttcg ccgggaacca ttcggagctg gctttcagac ccgccgggat catcgctgct
     5401 tccgcggcac cgaccgcacc acgagcaaca gccaggccac cccagctacc tgccagcgcg
     5461 gttacaccac agaacacggc ccacagcaca gcaaacattg cataaccgat tttcgtaccc
     5521 agcacatcca gtacataacc tgctaccggt tgcatgaccg tataagcagc agaataggct
     5581 gcgatgatat aggaatactg ttgggtggag atgtttaact cttccatcag agttggcgca
     5641 gctgccgcca cagtgttacg cgtcaggtaa ccaagcacgg tgccgagcgt caccagtgcg
     5701 atcatatacc aacgtaaccc tttaatttta cgcatgtaaa acctcatcgt tttgttatat
     5761 ccgcaggaag cgcggccatc atcccgccag aggctgaaga tgttagttac gaccgggtaa
     5821 ccgaaaaaaa accggcacgc cccgaacgtt gccatgaaag tttcgtgcta attcggcttc
     5881 cgtaccggtt aacccgattc atctaacaaa tgaatgaatc ggcaatttgc attccgtcga
     5941 cttatgattt gcgacggcag aaagataact tgtcatacaa ctttaaaagg tgagagccat
     6001 cacaaatgtg cgaatatttg tagggacatt acctgacgac agcgaggcca gtactggcgc
     6061 ggcctgcagc gagatttatc actttgagag taattttttt aactacgttt attgatctaa
     6121 ctcacgaaaa tatcttcgga ctctggaaat tggtgtgata actttgtcag catcgcacca
     6181 taagcaagct agctcactcg ttgagaggaa gacgaaaatg actccgttta tgactgaaga
     6241 tttcctgtta gataccgaat ttgcccgccg tctgtatcac gactacgcaa aagaccagcc
     6301 gattttcgat taccattgcc atttgccgcc gcagcagatt gcggaagact atcgttttaa
     6361 aaacctgtat gacatctggc tgaaaggcga tcactacaaa tggcgcgcta tgcgtaccaa
     6421 cggtgtggcc gagcgtctgt gtaccggtga tgcatctgac cgtgaaaaat ttgacgcctg
     6481 ggcggcgact gttccgcaca ctatcggcaa cccgttatac cactggacgc accttgaact
     6541 gcgtcgtccg tttggtatca ctggcaaatt gctttctccg tcaactgccg atgaaatctg
     6601 gaacgaatgt aacgaactgc tggcgcagga taacttctcc gcgcgcggca tcatgcagca
     6661 gatgaacgtg aaaatggtcg gcaccaccga tgacccgatc gattctctgg agcatcacgc
     6721 agagatcgcc aaagacggct ctttcaccat caaagtgctg ccgagctggc gtccggacaa
     6781 agccttcaac atcgaacagg cgacctttaa cgactacatg gcgaagctgg gcgaagtttc
     6841 cgataccgac attcgccgct ttgctgacct gcaaactgcc ttgaccaaac gtctggatca
     6901 cttcgccgct cacggctgta aagtgtctga ccatgcgctg gacgtggtga tgtttgctga
     6961 agcgaacgaa gcggaactgg acagcattct ggcgcgccgt ctggctggcg aaaccctgag
     7021 cgagcacgaa gtggcacagt tcaaaactgc ggtactggtg ttccttggtg ccgaatatgc
     7081 acgtcgcggc tgggtacagc agtaccacat tggcgcgctg cgtaataaca acctgcgtca
     7141 gttcaaactg ctggggccgg atgtaggctt tgactccatc aacgaccgtc cgatggcgga
     7201 agagttgtct aagctgctga gcaagcagaa cgaagaaaac ctgctgccga aaaccattct
     7261 ctactgcctg aacccgcgcg ataacgaagt gctgggcacc atgatcggta acttccaggg
     7321 cgaaggtatg ccgggcaaaa tgcagttcgg ttccggctgg tggtttaacg atcagaaaga
     7381 cggtatggaa cgtcagatga cccaactggc gcagctcggt ctgctgagcc gctttgtcgg
     7441 tatgctgact gacagccgta gcttcctgtc atacacccgt cacgaatact tccgccgcat
     7501 tctgtgccag atgatcggtc gctgggtgga agcgggcgaa gcaccggcgg acatcaacct
     7561 gctgggcgag atggtgaaaa atatttgctt taacaatgcg cgtgactact tcgccattga
     7621 actgaactaa ggtctggggt tgatatgcaa tacatcaaga tccatgcgct ggataacgtc
     7681 gcggtcgctt tagcagattt ggctgaaggc acagaagtca gtgtcgataa ccagactgtt
     7741 acgctgcgcc aggatgttgc tcgtggacat aaatttgcgt taacggatat cgcaaaaggg
     7801 gccaacgtca tcaaatatgg cctgccgatt ggttatgcat tggcggatat tgcggcgggg
     7861 gaacacgtcc acgcccacaa tacgcgcacg aatctgagcg atctggatca gtatcgctat
     7921 caacctgatt ttcaggatct gcctgcgcaa gcggcagatc gtgaagtgca gatctatcgt
     7981 cgcgctaacg gcgatgtcgg ggtgcgtaac gagctgtgga tcctgccaac cgtgggctgt
     8041 gtcaacggca tcgcgcggca aatccagaac cgtttcctga aagagaccaa caacgccgaa
     8101 ggtaccgacg gcgtgttcct cttcagccac acctacggct gctcacaact gggcgacgat
     8161 cacatcaaca cccgcaccat gctgcaaaac atggtgcgcc acccgaacgc gggcgcagtg
     8221 ctggtgattg gtctgggctg tgaaaacaac caggttgccg cattccgtga aacgttgggc
     8281 gatatcgatc ctgaacgcgt tcatttcatg atctgccaac agcaggatga tgagatcgaa
     8341 gccgggatcg agcatttgca tcagctgtat aacgtgatgc gcaacgataa acgcgagcca
     8401 ggcaaactca gcgaactgaa gtttggtctg gagtgcggtg gttctgacgg tctttctggt
     8461 attactgcta acccgatgct ggggcgtttc tctgactacg tgattgctaa cggcggtact
     8521 accgtactga ccgaagtgcc ggagatgttt ggcgcagagc agttgctgat ggaccattgc
     8581 cgcgacgaag caacgtttga aaaactggtc accatggtca acgactttaa acagtacttt
     8641 attgcccatg atcagccgat ctacgaaaac ccatcaccgg ggaacaaagc gggcggtatc
     8701 accacgctgg aagacaaatc acttggctgt acccagaaag cgggttccag tgtcgtggtt
     8761 gacgtgctgc gttacggcga gcgtctgaaa acgcctgggc tgaacttgtt aagtgcgccg
     8821 ggtaacgatg ccgtagcgac cagcgccctg gcgggtgcgg gctgccatat ggtgctgttc
     8881 agtactggtc gcggcacgcc atacggtgga tttgtgccga cggtgaaaat cgccaccaac
     8941 agtgaactgg cggcgaagaa aaaacactgg atcgactttg acgcgggtca gctgatccac
     9001 ggtaaagcga tgccgcagtt gctggaagaa tttatcgaca ccatcgttga gtttgccaac
     9061 ggtaagcaaa cttgcaacga gcgtaacgac ttccgcgaac tggcgatctt caaaagcggc
     9121 gtaacgctat aaagtcgaaa tgagttgtta ataaaaaacg gcgtttcata atgtgaagcg
     9181 ccgtttgctt ttccatacct gataaggatt tatcatgacc gcgtattggc tggcccaggg
     9241 cgtgggtgtc atcgcctttc tgattggtat cacaacattt ttcaatcgtg acgaacgtcg
     9301 cttcaaaaag cagctttcgg tctatagcgc cgttattggc gtacattttt ttcttctggg
     9361 cacctatccc gctggtgcca gtgccatcct taatgccatt cgtacattga ttaccttacg
     9421 cacgcgcagc ttatgggtaa tggcgatttt tattgtgctg actggcggaa ttggcctcgc
     9481 gaagttccat catcctgtcg aactattgcc ggttatcggt acgattgtca gtacctgggc
     9541 gctgttccgc tgtaaagggc tcaccatgcg ctgcgtgatg tggttttcaa cgtgttgctg
     9601 ggtgattcac aacttctggg cggggtcgat aggcggcaca atgattgagg gtagttttct
     9661 gcttatgaat ggcttgaata tcattcgttt ctggcggatg cagaaaaggg gaattgatcc
     9721 gtttaaagta gagaaaaccc ctcccgccgt agacgaaagg ggttaaacaa ttaattacgc
     9781 agggcgctat ttgccagacg atcgtcttcc gcctggcaag ctgccgcagt gaacagcacg
     9841 tcagttgaag agttcagcgc ggtttcgcaa gagtcctgca atacgccgat gataaagccg
     9901 acggcaacca cctgcatggc gatatcgttc gaaataccga acatattaca ggccagcggg
     9961 atcagcagca gagaaccccc cgccacgccg gatgcgccac aggcacacag agaagccacc
    10021 acgctcaaca gcagcgccgt gggcagatcg accgggatac ccagcgtatt aaccgcagcc
    10081 agcgtcaaca cggtaatggt gattgctgcg cccgccatat tgatggtggc acccagcgga
    10141 atagaaacgg aataggtatc gcgatccaga ttcagctttt cacacagtgc catattcacc
    10201 ggaatgttcg ccgcagagct gcgggtgaag aaggcataca cgccgctttc gcgcaggcac
    10261 agcagcacca gcgggaacgg gttacgacga attttccacc acaccagcaa tgggttaacc
    10321 accagcgcca ccagtaacat acagccaacc agcacgacca gcagttgcgc gtagccccac
    10381 agtgtggaga aaccggtggt tgccagggta gaagaaacca gaccaaaaat accaatcggt
    10441 gcgaagcgaa tgaccagttt caccataaag gtgacggcat tcgacatatc gttgaccagg
    10501 tttttggtgg tctcgttacc gtgacgcagt gcgaaaccga ggccaattgc ccacaccagg
    10561 atcccgatgt agttaccttt cagcagcgcg tcgatggggt tggaaaccat gctcattacc
    10621 agcccgcgca tcacttctac aatgcctgac ggtggcgaaa tatcacccgc gctactggat
    10681 aagtgcaggg tagaagggaa ggcgaagctg aaaacgactg cggccagagc agcagagaag
    10741 gtacccagca gatagaggaa caaaataggg cggatattgg ttttctgccc gtgctggtgg
    10801 ttagcaatag atgccatcac cagcatcaac accaggatgg gggcaacggc tttcagtgcg
    10861 ccgacgaaca aagtacctaa cagaccaaca gcttccgccg cgggttttga tatccatgcc
    10921 agaagaatcc ccagaacaag gccgaccagg atttgtttta ccaggctgcc atgagccaga
    10981 cgccggaata gccccggtga acgttgcgta gtcatttttc gatcctttca ttgtgttatc
    11041 gcacatccct ggtgcgttct gtcgacgcat ctttcaggat gtaacaaagt gtttgcaagg
    11101 tcgagtataa ggaaagttac agcttcggga agcgaaaaat gctggatttt aaagagaagt
    11161 catattttga aacttaatgg ttacatttgt ttgacataaa cagagggaag gactggctac
    11221 taaggctgat tagataacat atgggcaaaa ctatttggaa tatgagaacg gttgcgctcc
    11281 tttaatgtgc tatgccatat aaagtcactg g
//
LOCUS       DAZRUF010000132        11310 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZRUF010000132 DAZRUF010000000
VERSION     DAZRUF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11310
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.132"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            79..213
                     /locus_tag="ACHXOL_002771"
     CDS             79..213
                     /locus_tag="ACHXOL_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR6809705.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            192..296
                     /gene="speFL"
                     /locus_tag="ACHXOL_002772"
     CDS             192..296
                     /gene="speFL"
                     /locus_tag="ACHXOL_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR6809706.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            607..2805
                     /gene="speF"
                     /locus_tag="ACHXOL_002773"
     CDS             607..2805
                     /gene="speF"
                     /locus_tag="ACHXOL_002773"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR6809707.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2802..4121
                     /gene="potE"
                     /locus_tag="ACHXOL_002774"
     CDS             2802..4121
                     /gene="potE"
                     /locus_tag="ACHXOL_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR6809708.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHXOL_002775"
     CDS             complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHXOL_002775"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR6809709.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHXOL_002776"
     CDS             complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHXOL_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR6809710.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHXOL_002777"
     CDS             6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHXOL_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR6809711.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHXOL_002778"
     CDS             7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHXOL_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR6809712.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7910..8440
                     /gene="fldA"
                     /locus_tag="ACHXOL_002779"
     CDS             7910..8440
                     /gene="fldA"
                     /locus_tag="ACHXOL_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR6809713.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8650..8736
                     /gene="uof"
                     /locus_tag="ACHXOL_002780"
     CDS             8650..8736
                     /gene="uof"
                     /locus_tag="ACHXOL_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR6809714.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8729..9175
                     /gene="fur"
                     /locus_tag="ACHXOL_002781"
     CDS             8729..9175
                     /gene="fur"
                     /locus_tag="ACHXOL_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR6809715.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHXOL_002782"
     CDS             complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHXOL_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR6809716.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHXOL_002783"
                     /pseudo
     CDS             complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHXOL_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2769 a         2780 c         2815 g         2946 t
ORIGIN      
        1 tcttaacgca aatataaaac acgaaattac aacatttaag taacaaaaca gcatctaata
       61 tggcgatatt cataattaat gatttattat ctggggtatg atcgtttttt gttgatcttc
      121 ttcacagatt atagccattt catggataga ataactctac cttcaactga cacagcaaga
      181 ggtaaaggta aatggaaaat aataaccgct taatgcctca tataaggcgg acaacccata
      241 tcatgaagtt tgcccatcgt aatagttttg actttcattt ctttaatgcc cgctagtctt
      301 ctgactaaag ggcaccccaa cgtacaggtc tccctgactt taagcattac aggttaatac
      361 ctgtattcct cggtgctcat atactgctaa cccttttaaa ctctaaataa ttcgagtcgc
      421 agcacttgca acttgaggta tgacgagtat agccagttac cgggctggtc tgggttattg
      481 catctgcaaa aagcaaacta ctgatttatt tatcagcggt ggagctttgc tttttttccg
      541 gcgtgatcga tttctccttt gagaaattga ggacctgcta ttacctgaaa taaagagatg
      601 aataaaatgt cagaattaaa aattgccgtt agccgttctt gcccggattg tttttccact
      661 catcgtgcat gcgtgaatat tgacgaaagt aattatattg acgttgccgc cattatttta
      721 tcagtcaatg atgttgaacg tggaaaactc gatgaaatag acgctactgg ctatggcatt
      781 cctgttttta ttgcaacgga aaatgaagaa cgtgtaccag cagaatatct gccacgtatt
      841 tctggtgtct tcgagcattg cgaatcacgg aaagagtttt atggacgtca gttagaaacc
      901 gctgctagcc attatgaaac gcaactgcgc ccgcccttct tccgcgcact ggtcgattat
      961 gtcaatcagg gcaatagcgc ttttgactgc ccgggtcatc agggaggtga gtttttccgt
     1021 cgtcatccgg cagggaatca gttcgtggag tacttcggag aaacgctgtt ccgttccgac
     1081 ttatgcaacg cagacgtagc aatgggcgat ctgctgattc acgaaggcgc gccatgcatt
     1141 gcacagcaac atgcggcaaa agtgtttaat gccgataaaa catacttcgt tctaaatggc
     1201 acttcatctt ctaacaaagt ggttttaaac gccctgctaa caccgggtga tctggtgctg
     1261 tttgatcgca ataaccacaa atctaaccac cacggagcgt tgctacaggc tggtgcaaca
     1321 ccggtttatc tggaaacggc acgtaacccg tatggcttta tcggtggcat tgatgcgcac
     1381 tgttttgaag aaagttacct gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa
     1441 gaggctcgtc ctttccgcct cgctgtgatt cagttaggca cctacgacgg tacgatttat
     1501 aacgcccgcc aggtggtgga taaaattggt catctgtgtg actacattct gtttgactcc
     1561 gcatgggttg gctatgagca atttatcccg atgatggctg actgttcgcc gctgttgctg
     1621 gatcttaatg agaacgatcc gggtattctg gttacgcaat ccgtacataa acagcaggct
     1681 ggtttttctc aaacttcaca aatccataaa aaagacagcc acatcaaagg gcaacagcgt
     1741 tatgtaccgc acaaacgcat gaacaacgcc tttatgatgc acgcctccac cagcccgttc
     1801 tatccgctgt ttgccgcact ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt
     1861 aatatgtgga tggattgtgt ggtaaatggc attaatgccc gcaaactgat cctcgataac
     1921 tgtcagcata ttcgtccgtt cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat
     1981 gaaacagcgc aaattgcggt tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg
     2041 cattcttttg aaggctatgc agagaatcaa tactttgtcg atccttgcaa actgttgctg
     2101 acaaccccag gtattgatgc acgtaacggc gaatatgaag cgttcggtgt acccgcgacg
     2161 attcttgcta acttcctgcg cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc
     2221 atcctcttcc tgctgactcc ggcagaagat atggccaaac ttcagcaact tgttgccctg
     2281 ctggtacgct tcgaaaaact gcttgagtcc gacgcgccat tagcagaagt gctgccttcc
     2341 atctacaaac agcatgaaga gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa
     2401 atgcatgatt tgtatgcccg acacaacgtg aaacaactgc aaaaagagat gttccgcaaa
     2461 gcgcacttcc cacacgtcag catgaatccg caagaagcca actacgccta tttacgcggt
     2521 gaagtggagc tggttcgtct gccggatgcc gaaggtcgta tcgctgccga aggggcgctt
     2581 ccttatcctc cgggtgtgct gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg
     2641 cgttacttca gcgctctgga agaagggatc aacctgctgc caggttttgc accggagctg
     2701 cagggtgtct atatcgaaga acatgatggt cgtaagcaag tttggtgcta tgtcatcaag
     2761 cctcgtgatg cgcaaagcac cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga
     2821 acaaaatggg cgtcgttcag ttaaccatac tgacgatggt caacatgatg ggctccggta
     2881 tcatcatgct gccgacaaag cttgccgaag ttgggacaat ttcaattatc tcctggctgg
     2941 tgacagccgt aggctcaatg gcactggcat gggcattcgc aaaatgcggt atgttcagcc
     3001 gtaaatcagg tggtatgggc ggttatgccg agtatgcttt cggtaaatcc ggtaacttta
     3061 tggcgaacta tacctacggc gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg
     3121 cggttggtta cggcaccgaa ctgctcggcg cgagtttgtc gccagtgcag attggtcttg
     3181 cgaccatcgg ggtgctgtgg atttgtaccg tggctaactt tggtggtgcg cgcattaccg
     3241 ggcaaatcag tagcattacc gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta
     3301 tcggctggtt ctggtttagc ccgacgcttt acgttgattc ctggaatccg catcatgcac
     3361 cgttcttcag tgcagtaggt tcttccatcg ctatgacgct gtgggctttt ctcggtctgg
     3421 agtctgcgtg tgcgaatact gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg
     3481 tactcggcgg tacgttaggt gcggcggtga tttatatcgt ctccaccaac gtgattgcag
     3541 ggattgtgcc taatatggag ctggcaaatt caacggcacc atttggcctg gccttcgcac
     3601 agatgttcac accagaagtg ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg
     3661 gttcgctgct tggctggcag ttcaccattg cccaggtgtt taagtcttca tctgatgaag
     3721 gctacttccc taaaattttc tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt
     3781 tgaccattgt gattatccag agcgggttgg cgctgatgac gattagcccg tcgctaaaca
     3841 gtcagttcaa cgtgctggtt aacctggccg tggtgaccaa tatcattccg tatattctgt
     3901 caatggcggc attagtcatt attcagaagg tcgctaatgt gcccccctca aaagcgaaag
     3961 ttgcaaactt tgttgctttt gttggcgcga tgtatagctt ttatgcgctg tactcatccg
     4021 gggaagaagc catgctgtac ggttccatcg tgaccttcct tggttggaca ctgtatggtc
     4081 tggtatcacc acgctttgaa ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg
     4141 gcgatcttgc gaccgccctt tttttattaa atgtgtttac gcgtttttca gaacttcgct
     4201 aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga ggaagctttc
     4261 gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc
     4321 agtcatcact ttcagaccgc cgatagaagc accattgccc ggtgccgccg tcaggcgcgc
     4381 ggtgatcggg tcgcctgcca gggtgctggc gctcaccatt tccggagaca gcttagacag
     4441 cgccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa
     4501 gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag cggtgatttc
     4561 cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg tgccgtcgaa
     4621 acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag
     4681 accatcaaca aaccatttga aacctaccgg gacttctacc agcttgcggc ccaagtcatt
     4741 gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa catctttgcc
     4801 ccactgcgga cgatgctgga acaggtagtt gatcgccacc gccaggtagt ggttcggatt
     4861 catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt tagcaaacgc
     4921 cagatcgaac ttatcacgca atgccagcag gcccgccatc gcacactcgg aggagcagtc
     4981 catacggatc gcgccgtctt tatcgaggtg cataaagcgg aaggtttgat cgacctgatc
     5041 gttaacgatg gtcaggttga ggttgtaata ctcgccaatt cgcttccagt attcgatacc
     5101 ggaaccaccc agcggatcaa cgcccagcgt caggcccgtt ttctgaatcg ccgccatatc
     5161 aacgatatcg gccagacctt ccacaaactg ctgcaccaga tcctgctctt tcacatgacc
     5221 ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat cggccatcag
     5281 tgcgttggca cgatcttcca ccactttagt gacgttggta tcagccgggc caccatttgg
     5341 cggattgtac ttgataccac catcttccgg cgggttatgg gagggtgtaa tcacgatacc
     5401 gtctgccagc gggccacctt ttttattatg aaccaggatg gcattggaaa cggcaggcgt
     5461 cggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg ccagcacttc
     5521 cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca catagcaagg
     5581 gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg
     5641 cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac cgaatttcac
     5701 cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg tcagttgggc
     5761 gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga ttgccattgc
     5821 tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc aattccgccg
     5881 ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg gtgttggtgt
     5941 tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga ttaccatttt
     6001 tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc aacgattccg
     6061 ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc agcataaagc
     6121 gattgaccgc tcgcttttgc tctgcgtatt cgtcagaaag cagaagttca cgcattgcgc
     6181 gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc
     6241 gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc
     6301 gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata tagctgtaga
     6361 gttcatcatc aacttcaatc gttttcatct taatccagta cagtgtctta gctgaataca
     6421 agtcgccaag agtatatgaa taaatcccgg cagcggatag cgtcaagcct ggcttgcaga
     6481 aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa cataataccc
     6541 taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc gcgcaaactg
     6601 cacaaaacca gcacaataat tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg
     6661 acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc caggttgata
     6721 tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg atggcgcagg
     6781 atcttgttga tactctggat gcacagcaga tcgacaaagc aacatttatc ggtcactcca
     6841 tgggcggtaa agcggtaatg gcacttacgg cactggcccg cgatcgcatc gataaactgg
     6901 tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag attttcgcgg
     6961 ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca gcaataatgc
     7021 accagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt gttgacgggg
     7081 agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg ggttgggaga
     7141 aaatcccggc atgggatcac cctgccctgt ttatccctgg cggcaattct ccgtatgtta
     7201 gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg catgtgattg
     7261 caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc gctatccgtc
     7321 gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg
     7381 tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat ccgggcagag
     7441 gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt
     7501 acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac taatccgctt
     7561 tcgcgcgatg aacagctgcg tattaataaa cgcaaccagc taaaacgcga caaagtacgt
     7621 ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct gaacgagctg
     7681 gcggagtcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg
     7741 gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca cagtgtgcag
     7801 tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc tttatccgtg
     7861 ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca tggctatcac
     7921 tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa
     7981 acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca aagaagatct
     8041 ggaagcttat gacattctgc tgctgggcat cccaacctgg tattatggcg aagcgcagtg
     8101 tgactgggat gacttcttcc cgactctcga agagattgat ttcaacggca aactggttgc
     8161 gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg cattgggcac
     8221 catccgcgac atcattgaac cgcgcggtgc aacgatcgtt ggtcactggc caactgcggg
     8281 ctatcatttc gaagcatcaa aaggtctggc tgatgacgac cactttgtcg gtctggctat
     8341 cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat
     8401 ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg cgtagactca
     8461 tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat ttgtaacttt
     8521 tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt aaatgtaagc
     8581 tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt taggcgtggc
     8641 aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc taatgaagtg
     8701 aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc taaagaaagc
     8761 tggcctgaaa gtaacgcttc ctcgtttaaa aatcctagaa gttcttcagg agccggacaa
     8821 ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg aagaaattgg
     8881 tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg tcacccgcca
     8941 caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc acgatcacct
     9001 gatctgcctc gactgcggca aggttatcga atttagtgat gattccatcg aagcgcgtca
     9061 gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct atctttacgg
     9121 tcactgtgcc gaaggcgatt gccgcgaaga tgaacatgcg cacgaaggca aataagccag
     9181 cctgaatgag aaaaagccaa cctgcgggtt ggctttttta tgcaagggaa ataagaaaag
     9241 cgagagttac agctctcact tatttgttat tactgcgaat ttcctgccaa actttatcac
     9301 aatcggcttt caccgcctga tcatttccgg tttgcgttgc acgcagacac tgctgataat
     9361 ccagtacgcg gacactttcc tggtcggcaa aatgctgatg ttgtttctct ttcttcagca
     9421 cgtttaacac gctctggcag gcttcaattt tttctggcga accttgtgcg gtgttgatac
     9481 aggcgctgta tgcctctttc agacgactgt cttcctgtgg cgcggttgat tgcgcacaag
     9541 ctaccagccc cgatgccatt atggcgatga gaatcagttt tttcatagca aattcctcaa
     9601 acgtgcggca ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt
     9661 accataaatt tcacgtcacg ctcatcctgg aagatgttgc cgtaaccacc gccccagctt
     9721 gggatgtcgg agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg
     9781 cgaccgtcct gaatggtgta gaccgcatcc aggctgtatg cagactcttc aatagtccgg
     9841 tttttgtcgt agtacgcatc cggattgctc tgctaggtcg caggtttagc atcccatgcg
     9901 taaacgtagg aagcgccgat ggcgaagcct ggaagattcc agtttttcag gtcatacatc
     9961 gcaccgaaga agaccgcttt ttcgccgtta gcgttgaagt cggaacggtt atcccaccag
    10021 atatccaggc ggccgttgga ggaagcgtag gttggagtca tacgttgcag gaagtatccc
    10081 tgctgaccgt cagccttaac ccaggtgcct tcgaggcgca aatccactac gtcagccgcc
    10141 cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt cataaaggtc gttgacgctg
    10201 cgatcgtcaa ctttatcgcg cgtaccatag aactggtagc tggtggttaa cgggctaccg
    10261 gcgatatcaa atttgtagct ggctttggca aaatattgat cgatataccc ttccgcctga
    10321 ccaaatgccg cttccagtac gaagttattt ttgaagtcgt atttcgcccc aagggagtgc
    10381 agataatcaa ctttggtggt tttatcgttc tgataaaact catccatttc cagatgccac
    10441 ggcgctttgt attcgttggt ccacatgtag gagaaactca acgcaccagc atcgccgtaa
    10501 tcaaaattcg ccccggcttc cgccccctga taagtacctg gcataaagct ccagtgcggc
    10561 gctaacagcg tttgaccagt tggctgaatg taacctgccc tcgcccaaac cggaccgtat
    10621 ttaaatttgg ccgcagcttt atacaggctt ataccgcttt tgtcgccgga ccagtcttcg
    10681 tcataggctt tattactttt tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt
    10741 tccgccattt caatcgccgt aaacgcagca atatcaaggc cgaacatatc agcagcataa
    10801 ccggactgaa aatcgaggtt ggcattccag gtggagtgag aaaggttggt tttgtatttg
    10861 tcgccgtcgg taacatcttt acggtcgcgt tcacgctgcc agtaatatat accgccggtt
    10921 aaggttgaat cgtcgatgaa tccttcggcg cgagcctccg gcattgccat aaagcccgac
    10981 attgctgtaa caccggcgat agccagcgcc agcgtactat gtttgccact aaacgtacgc
    11041 atgggttaat cctctttgac gtataaattg ctgcaccaaa ggtgaacagc gaaaaaataa
    11101 aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac tatttttcgc
    11161 gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata
    11221 ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaataaa
    11281 agcgtgaaat tccagcaaga tgaaagcggt
//
LOCUS       DAZRUF010000133        11244 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZRUF010000133 DAZRUF010000000
VERSION     DAZRUF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.133"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..311
                     /locus_tag="ACHXOL_002784"
     CDS             <1..311
                     /locus_tag="ACHXOL_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR6809717.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            510..791
                     /gene="ppiC"
                     /locus_tag="ACHXOL_002785"
     CDS             510..791
                     /gene="ppiC"
                     /locus_tag="ACHXOL_002785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR6809718.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(878..2353)
                     /gene="ilvC"
                     /locus_tag="ACHXOL_002786"
     CDS             complement(878..2353)
                     /gene="ilvC"
                     /locus_tag="ACHXOL_002786"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR6809719.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2503..3396
                     /gene="ilvY"
                     /locus_tag="ACHXOL_002787"
     CDS             2503..3396
                     /gene="ilvY"
                     /locus_tag="ACHXOL_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR6809720.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3448..4992)
                     /gene="ilvA"
                     /locus_tag="ACHXOL_002788"
     CDS             complement(3448..4992)
                     /gene="ilvA"
                     /locus_tag="ACHXOL_002788"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR6809721.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTLLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4995..6845)
                     /gene="ilvD"
                     /locus_tag="ACHXOL_002789"
     CDS             complement(4995..6845)
                     /gene="ilvD"
                     /locus_tag="ACHXOL_002789"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR6809722.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6910..7839)
                     /gene="ilvE"
                     /locus_tag="ACHXOL_002790"
     CDS             complement(6910..7839)
                     /gene="ilvE"
                     /locus_tag="ACHXOL_002790"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR6809723.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7859..8122)
                     /gene="ilvM"
                     /locus_tag="ACHXOL_002791"
     CDS             complement(7859..8122)
                     /gene="ilvM"
                     /locus_tag="ACHXOL_002791"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR6809724.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8119..9765)
                     /gene="ilvG"
                     /locus_tag="ACHXOL_002792"
     CDS             complement(8119..9765)
                     /gene="ilvG"
                     /locus_tag="ACHXOL_002792"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR6809725.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9768..9818)
                     /gene="ilvX"
                     /locus_tag="ACHXOL_002793"
     CDS             complement(9768..9818)
                     /gene="ilvX"
                     /locus_tag="ACHXOL_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR6809726.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9905..10003)
                     /gene="ilvL"
                     /locus_tag="ACHXOL_002794"
     CDS             complement(9905..10003)
                     /gene="ilvL"
                     /locus_tag="ACHXOL_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR6809727.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10356..>11244
                     /locus_tag="ACHXOL_002795"
     CDS             10356..>11244
                     /locus_tag="ACHXOL_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFR6809728.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGRHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNG"
BASE COUNT         2554 a         3090 c         2951 g         2649 t
ORIGIN      
        1 ttgtcgtgct caacggaaaa attaatggca acgcatgtat cgtcgttcca ttatcaacca
       61 ctcgcgatca tgacaagtta aatcgtggaa tgcatgttga aatagccagt aatgttatca
      121 atgacctgca attctttgat cagcagattc gttgggccaa agccgaccta gtacaacaag
      181 tcagtcgaaa ccgattaaac agagcaagga cttacagagg gtatcttaat caatgtttgc
      241 cacatgagtt ggttgcagac atacagaggg cggtcatcaa gtcaatcaat gccatatcgc
      301 tcataaattg acaataaaat aaccatcccc gataatgact ttgtagccca tagaggccct
      361 aagttttctc cccttgaggg acctgcgaag cccggatttt ccgggctttt gccatatatg
      421 taccagcccc tccctgacta acaattacgt tagcatctgc ggatcatgct aaaatcgccg
      481 cctgacaatt attcactcaa ggcacgatca tggcaaaaac agcagcagca ctgcatatcc
      541 ttgtaaaaga agagaaactg gctctggatc ttctcgagca gattaagaac ggtgccgatt
      601 tcggcaagct ggcgaagaaa cactccattt gcccatcagg caaacgcggc ggtgatttag
      661 gtgaattccg ccagggtcag atggttccgg cgtttgataa agttgtgttc tcttgtccgg
      721 tactggagcc aaccggcccg ctgcacaccc agttcggtta ccacatcatt aaggtgctgt
      781 accgtaacta atagcaaggc cttctccagg agaaggcctt gagtgttttc tccctctccc
      841 tgtgggagag ggtcggggtg agggcatcag cgcgcactta acccgcaact gcaatacgtt
      901 tcatatccgt catatagccg cgcagtttct tacctacctg ctcaatcgca tggcagcgaa
      961 tcgcttcgtt cacatcacgc agttgcgcgt tatctaccgc accttccgga atagctttac
     1021 ccagatcgcc cggttgcagc tctgccataa acggtttcag cagcggcaca caagcgtaag
     1081 agaacagata gttaccgtac tcggcggtat cagagataac cacgttcatt tcgtacagac
     1141 gcttacgggc gatggtgttg gcaatcagcg gcagttcgtg cagtgattca tagtaagcag
     1201 attcttcgat gatgccggaa tcaaccatgg tttcaaacgc cagctcaacg cccgctttca
     1261 ccatcgcgat catcagtacg cctttatcga agtactcctg ctcgccgatt ttgccttcat
     1321 actgcggcgc ggtttcgaag gcggttttgc cggtctcttc acgccaggtc agcagtttct
     1381 tatcatcgtt ggcccagtcc gccatcatac cggaggagaa ttcgccggag atgatgtcgt
     1441 ccatatgttt ctggaacagg ggtgccatga tctctttcag ctgttcagac agcgcgtaag
     1501 cacgcagttt cgccgggtta gagagacggt ccatcatcag ggtgatgccg ccctgcttca
     1561 gcgcttcggt gatggtttcc caaccgaact gaattagttt ttctgcgtat gccggatcgg
     1621 tgccctcttc caccagcttg tcgaagcaca gcagagagcc agcctgcaac ataccgcaca
     1681 ggatggtttg ctcacccatc aggtcagatt tcacttccgc aacgaaggac gattccagca
     1741 cgcccgcacg gtgaccaccg gttgctgccg cccatgcttt agcgatcgcc atgccttcgc
     1801 ctttcggatc gttttccggg tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt
     1861 actcttcacg tacttcggtg ccagggcatt tcggcgcaac catgactacg gtaatgtctt
     1921 tacggatctg ctcgcccact tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc
     1981 cgtctttcat cagtggctgt acggtgcgca ctacatcaga gtgctgcttg tctggcgtca
     2041 ggttaaccac cagatccgcc tgcgggatca gttcttcgta agtaccaact ttaaaaccgt
     2101 tttcggtcgc tttacgccag gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt
     2161 aggagatatc gagaccagaa tcacgcatgt tcaggccctg gttcagcccc tgtgcgccac
     2221 agccgacgat aaccactttt ttaccctgaa ggtagctcgc gccgtcggcg aattcgtcgc
     2281 gccccataaa gcgacatttg cccagctgtg ccagctgctg gcgcaggttc agtgtattga
     2341 agtagttagc catggtgatt cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg
     2401 cgagattgaa ttcactatat gacaggaaat ttattgcgga aattgatata ttcacaacgt
     2461 cacattgcaa tttctgcaac gtcaacatcg agggctgcgc cattggattt acgcgatctg
     2521 aaaaccttcc tgcatctggc ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac
     2581 gttagcccat ccacgctctc acggcagatt cagcgcctgg aagaagatct tggtcagccg
     2641 ctatttgtgc gcgataaccg cacggtaacg ctgacggaag cgggcgaaga gctgcgcgtt
     2701 ttcgcccagc aaacgctgtt gcagtatcag caattgcgcc acaccatcga tcagcaaggg
     2761 ccgtcgctct ctggcgaatt acatatcttc tgctcggtga ccgctgccta cagccatctg
     2821 ccgccgattc tggatcgctt ccgcgcggaa cacccgtcgg tggagatcaa actcaccact
     2881 ggtgatgcgg cagatgcaat ggaaaaagtg gtcacgggag aagcggatct ggcgattgcc
     2941 gggaaaccgg agacgctacc cggcgctgtg gcgttttcga tgctggagaa tctggcagta
     3001 gtgctgattg cccccgcgct gccctgcccg gtgcgtaatc aggtatctgt cgaaaagccc
     3061 gactggtcaa cggtgccgtt tattatggcc gatcaggggc cggtacgccg ccgcattgaa
     3121 ctgtggtttc gacgcaataa aatcagcaac ccgatgattt acgccacggt tggcgggcat
     3181 gaagcgatgg tatcgatggt ggcgctcggc tgtggtgtgg cgctgttgcc ggaagtggtg
     3241 ctggaaaaca gcccggagcc tgtacgcaac cgggtaatga ttttagatcg cagcgatgag
     3301 aaaacgccgt ttgaactggg cgtgtgtgca caaaaaaagc ggctgcatga gccgctgatt
     3361 gaggcgttct ggaggatttt gccgaaccac aaatgacgtt gtcgcgcggg taggcctgat
     3421 aagcgaagcg ctatcaggca tttttcccta acccgccaaa aagaacctga acgccggatt
     3481 attggtttcg tcgtggcaat cgtagcccag ctcatttagc cgggtttcga aatccggttc
     3541 atggtcgccg agttcgaacg ccgccagtac gcgcccgtag tcggtgccat ggctgcgata
     3601 gtggaacaaa gaaatgttcc agtgcgtacc cagcgtgttg aggaagcgca gcagcgcgcc
     3661 cggtgattcc gggaattcga agctgtagag gcgttcctgc aacggatgtg acggacgccc
     3721 gccgaccata tagcgcacat gcaacttcgc catttcgtcg tcggagagat ccaccacgct
     3781 gtagccgccg tcgttgagca tctgcaaaat ttctttgcgc tcttcgaggc cgcggctcag
     3841 gcgcacgccg acaaagatgc aggcgttttt ggcatcggca aaacggtagt tgaactcggt
     3901 gaccgatcgc ccgccaagca gttggcagaa tttgaggaag ctgccttttt cttccggaat
     3961 ggtcaccgcc aacaacgctt cacgctgttc gcccagttcg cagcgttccg agacgtagcg
     4021 caggccgtgg aagttcacgt tggcaccgga aagaatatgc gccaggcgtt cgccgcgaat
     4081 gttgtgctga gcgatatatt ttttcattcc cgccagcgcc aacgcgccag agggttccgc
     4141 cactgcgcgc acatcttcga ataaatcctt catcgccgca cagatcgcat cactatcgac
     4201 ggtgatgatg tcgtcgagat actcctggca taaacggaag gtttcgtcgc cgatgcgttt
     4261 taccgcgaca ccttcggcga acagtcccac gcgcggcaga tcaaccggat gacccgcatc
     4321 cagcgctgct ttcaggcagg cggagtcttc cgcttctacg gcgatcactt tgatttgtgg
     4381 catcagttgt ttgatcagca ccgcaacgcc cgccgccaga ccgccgccgc cgactggcac
     4441 aaatacgcgg tcgagatggg cgtcctgctg gagcagttcc agcgccagcg tgccttgccc
     4501 ggcaatcacc atcggatggt cgaacggcgg cacccaggtg aacccctgct gctgtgacag
     4561 ttcgatcgct ttagctttcg cttcgtcaaa gttcgcgcca tgaagcagca cttcgccgcc
     4621 gaatccacgt accgcatcga ctttgatgtc ggcggtggcg gttggcataa cgatcagggc
     4681 cttcacgcct aaccgcgcag aagaaaacgc gacgccctgc gcgtggttac ccgcagaagc
     4741 agtgatcacg ccgtgcgctt tttgttcttc cgtcaggccc gccatcatgg cgtatgcgcc
     4801 gcgcagctta aagctgtgca ctggctggcg atcttcgcgc ttcaccagaa tgacgttatc
     4861 aagacgcgac gacagttttt ccattttttg tagcagcgta acctgcgccg cctcgtaaac
     4921 cggcgcgcgc agcactgctc ttaaatattc ggcaccttcc ggagcaccgg acaggggttg
     4981 cgagtcagcc attattaacc ccccagtttc gatttatcgc gcaccgcacc tttgtcggcg
     5041 ctggttgcca ggctggcgta ggcgcgcagg gcaaaggaaa cctgacgttc acggtttttc
     5101 ggcgtccagg ctttgtcacc ccgagcttcc tgcgcttcac gacgcgccgc cagttcggca
     5161 tcgcttacct gtaactgaat gccacggttc gggatgtcga tagcgatcag gtcaccatct
     5221 tcaatcagac caatgctgcc gccgcttgcc gcttccggtg agacgtggcc gatggaaaga
     5281 ccagaggtgc cgccagagaa acgaccgtcg gtgatcagcg cacaggcttt accgagaccc
     5341 attgatttca ggaagctggt tgggtagagc atttcctgca tgccagggcc gcctttcggg
     5401 ccttcatagc ggatcaccac cacatcgccc gcgacaactt tgccaccgag gatcgcttct
     5461 accgcgtcgt cctggctttc gtacactttc gccgggccgg tgaatttgag gatgctgtca
     5521 tcgacgcccg ctgttttcac gatgcagccg ttttcagcaa aattaccgta gagcaccgcc
     5581 aggccgccgt ctttgctgta ggcgtgttcc agcgagcgga tacagccgtt tgcgcgatcg
     5641 tcatcgagag aatcccaacg gcaatcctgc gagaatgcct gtgtagtacg aatgcccgca
     5701 gggcctgcgc ggaacatatt ttttaccgcg tcatcctggg tcagcataac gtcgtattgt
     5761 tccagagttt gcggcaacgt caggccaagt acgtttttca catcacggtt cagtaacccc
     5821 gcgcgatcca gttcaccgag aataccgata acaccaccag cacggtgaac atcttccata
     5881 tggtattttt gggtgctcgg cgcaactttg cataactgcg ggactttacg cgaaagtttg
     5941 tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg ccgccgccag caggtgaagt
     6001 accgtgttgg tagagccgcc catcgcgata tccagcgtca tggcattttc aaacgccgct
     6061 ttgctagcaa tattgcgcgg cagtgcactt tcgtcgtttt gctcgtagta acgtttggtc
     6121 aattcaacaa tgcgtttacc ggcattgagg aacaactgct tacggtccgc gtgggttgcc
     6181 agcagcgagc cgttgcctgg ctgcgacaaa cccagcgctt cggtcaggca gttcattgag
     6241 ttagcggtaa acatcccgga gcaggaaccg caggttgggc aggcggaacg ttcaacctga
     6301 tcgctctggg agtcagaaac tttcgggtct gcgccctgga tcatcgcatc aaccagatcg
     6361 agcttgatga tctgctcgga cagtttggtt ttcccggctt ccatcgggcc gccggaaaca
     6421 aagatcaccg gaatattcag gcgcagggaa gccatcagca tccccggggt gattttgtcg
     6481 cagttagaga tgcagaccat ggcgtcggcg cagtgggcgt tgaccatata ctcaacggaa
     6541 tcagcgatca gttcgcgaga tggcagtgaa taaagcatcc ccccgtggcc catagcaatc
     6601 ccatcatcca ccgcaatggt gttgaactct ttggcaacgc cgccagccgc ttcaatttgt
     6661 tcggcgacca gtttaccgag atcgcgcaga tggacgtgac ccggtacaaa ttgggtgaac
     6721 gagttcacaa ccgcgataat cggcttgcca aaatcggcat cggtcattcc ggtggcgcgc
     6781 cacagcgcac gagcacccgc catattacga ccatgagtgg tggtggcgga acggtactta
     6841 ggcatacttt atttactccc agtgtctgtc tcgtaaatgg gacggtaagt gccgtcccat
     6901 tttttgtatt tattgattaa cttgatctaa ccagccccat ttatcttcgg tttcgccagt
     6961 gaagaggccg aagaaggctt gctgaatgca tttggtaacc gggccgcaac ggccttcacc
     7021 cacctgaata ccgtctacac tgcgcactgg cgtgatttct gccgccgtac cggacataaa
     7081 cacttcatcc gccaggtaca gggattcgcg cgacagcacc tgctcacgca cttcaattcc
     7141 cagctctttc gccagtttga tgatggcatc acgggtaata cccggcagcg cggatgaggt
     7201 aaacggtggg gtgaacagca cgccgtcttt cacttcaaac aggttttcac ctgcgccttc
     7261 agagatgtaa ccgttcacat ccagcgcgat accttcctga taaccgtggc ggcgcgcttc
     7321 gctacccacc agcagggaag agaggtagtt accaccggct tttgccgccg tcgggatggt
     7381 gtttggtgct gcgcggttcc aggaggaaac catcgcatcg atcccctgct ccagcgcttc
     7441 tgcgcccaga tacgctcccc acgggaaagc agcgataatc acgtcggttg agtatcccgc
     7501 tggcgggttt actcccatgc caacatcacc gacgaagatc agcggacgga tataggcgct
     7561 ggtgagatta tttttgcgga tcacgtcacg acaagcttcc atcagctcat caatgctctg
     7621 cgaaaccggg aagcgataga ttttggcgga gtcatgcaga cgctgcatat gctcacgatg
     7681 gcggaataca accggtcctt tgtgcgagtc gtagcaacgg atgccttcaa aaaccgaagt
     7741 gccatagtgc agcgcgtgcg acatcacatg caccttcgcg tcttcccagc gaaccatctc
     7801 cccattgaac caaatgtaat cagctttctt cgtggtcatt tttatattcc ttttgcgctc
     7861 aggcgcggat ttgttgtgat gtggttgtgc tctggcagat ggcaacgtgt gcgacgtcca
     7921 ccagtttatt taactgacta aacagtaagt cgaccgaccg tgggctggca acggtcaatt
     7981 cgatatttat attttgtgca tcgctggcgg cggccatatt cattgagcag acgtggaaac
     8041 cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc tggattgaag cgagccgata
     8101 cattgacctg atgttgcatc atgataattt ctccaacatt tctgaattac tggcgccagg
     8161 cggcaccagc ggccagacgt tctcaagttc gtcgattgag acatgaagca ggtatggccc
     8221 atcactgttc agcatggtgt cgagtgccgc ttcaacctgg tctttacggg tgatgtgttg
     8281 gccagggatg ccgaaggcgc tggctaacat gaggaaatcg gggttatcag taagggtggt
     8341 ttcgctgtat cgttcctgaa aaaacagttg ctgccattgt cgaaccatcc ctaaccgttg
     8401 gttatcgagt aagacgattt tcaacggtaa ctgcttgcgt tttacggtgc ccagctcttg
     8461 cacattcatc atgaaagagc cgtcaccgga gatacagaca acggtatcgt tcggtcgcgc
     8521 gacttgtgcg ccaaccgccg ccggtaaacc aaaacccatg gtacctaagc cgctggaggt
     8581 gatgaaattt tccgggcgag tgtgggcgat gtgctgcgca gcccacatct ggtgctgccc
     8641 cacatctgtg gtcacgacgc aatccgcagg tttacgatcc gacagttgtt ttaacaacaa
     8701 cggcgcgtag atagcgtcac cgggatggtc gtaacgccag gaatgttcat cacgcagctg
     8761 cgcgcagtat tgctgccagt cattgatatt taacggctgc tgtaatgctg gtaacagagc
     8821 atttaaatca ccctgtaatg ccacatgtgc ctgacgcagc ttgttcattt ctgccgggtc
     8881 gatatccata tggataacac tggcgtgtgg tgcgaaagta ttcagtttgc cggtcacccg
     8941 gtcatcaaaa cgtgcgccca cggcgatcag caggtcacac tcctgcaccg cgaagtttgc
     9001 cgctttgatg ccgtgcatcc ccagcatgcc cagatagtac ggataatctg cttctactgc
     9061 gcccagccct ttcagcgtac aggtggcagg catttttgtg gcagcgagaa attcacgcaa
     9121 agccggaact gcctgcgcca tacccacgcc accgccaacg tacagcatcg gtttttgcgc
     9181 ttttgccagc atctggcgcg cttgctcaac ttcggcatgt gggaaagtca cttcgttttc
     9241 aacggtggtg aaccacggtt ccaggtcacc gctggctaac tggatatctt ttgggatatc
     9301 gaccagaacc ggaccaggac gacctgagca ggcaacgtcg aatgcttcag ccatgatgcg
     9361 cggcaactct tccagcgact gcaccagaaa gctgtgcttg gtacaggcta acgacaatcc
     9421 taggacatcc acttcctgaa atgcgtcagt gccgataaac ggtgcggaca cttgaccggt
     9481 gatggcaaca acagggatgg aatctaacag tgcgtccgca agcccggtta tcaggttggt
     9541 tgcgcccgga ccagacgtgg cgatacatac gccagttttg ccggtagcac gggcataacc
     9601 gatagccgcc attgccgcac cctgctcatg tcggcatagc aagtgctcca cgccgccgtc
     9661 atacaatgca tcgtaaaccg gcataattgc gccacccgga taaccgaaaa cggtgttcac
     9721 accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca ttcatagtta gttccccgtc
     9781 ctgaatcttg agaaacagaa ttttgtgctg ttattcattg tctgctcctc ggttatgttt
     9841 ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg tcttagttcg ttaaggcttg
     9901 atctctaagc ctttcctcgt ccaagtgcag ccccgcacgg tgggataata atcaccacca
     9961 cgctaatcac gactaggcta atcactcgta gaagggctgt cattttgtct tttcttgcat
    10021 cttgttcgaa ggaatgccta aagagttacc atagattttg cgaatagcac aagatatttt
    10081 tgggcctatt tttatgatag ctttaataat ttcaatagaa gttgttgttt tttaaaaaga
    10141 aaatagtgag tggaaaaaat tcaatttcag tataaatgtt gttcccttcc tcgtagttca
    10201 tcctttgtcc gctgcatttt tgcgagcatc attccaccgc cagaacaact caacttcact
    10261 tgcacaaata aacaggaatc agccactaaa attcacgaat tagcgggcta cagcagcgat
    10321 atcttatgca cataatggtg actcaaggag ggcttatgtc actgtcaatt gttcataccc
    10381 gcgcagccct gggagtaaat gcgcccccga tcactgttga ggtacatatc agtaaaggtc
    10441 tacccggctt aacgatggtg ggcttaccag aaacaactgt aaaagaagct cgcgatcgcg
    10501 tgcgcagcgc cattatcaat agcggatatg aatatccggc gaaaaaaatc accatcaacc
    10561 ttgctccagc cgatctgcca aaagaagggg gacgatatga tttacctatc gccattgcgt
    10621 tgctggcggc ctcagaacag cttacagcca ataagttaga tgaatatgaa ttagtcggag
    10681 aactggctct tacaggcgct ctgcgtggcg ttcccggtgc aatctccagt gcaactgaag
    10741 ctattaagtc gggcagaaaa attatcgtcg cgaaagataa cgaagatgaa gtggggctaa
    10801 ttaacggtga aggatgcctg atagccgatc atctgcaagc tgtctgtgcg tttctggaag
    10861 gtaagcacgc tctcgaacgc ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg
    10921 atctcagtga tgttgtcggt caggaacaag gaaagcgagg actggaaatt accgccgctg
    10981 gcaggcacaa ccttttactg attgggccgc cgggaacagg taaaacaatg ctcgccagcc
    11041 gtattaatgg cctgttgcca gatttaagca atgaagaggc actggagagc gctgcgatat
    11101 taagtctggt aaatgctgaa tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac
    11161 ctcatcacag tgcgtcgtta actgcgatgg tgggcggtgg tgcaattcca gggccaggtg
    11221 aaatttcgtt ggcgcataac ggcg
//
LOCUS       DAZRUF010000134        11189 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZRUF010000134 DAZRUF010000000
VERSION     DAZRUF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11189
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.134"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            53..352
                     /locus_tag="ACHXOL_002796"
     CDS             53..352
                     /locus_tag="ACHXOL_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR6809729.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(472..651)
                     /locus_tag="ACHXOL_002797"
     CDS             complement(472..651)
                     /locus_tag="ACHXOL_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR6809730.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            725..2086
                     /gene="pabB"
                     /locus_tag="ACHXOL_002798"
     CDS             725..2086
                     /gene="pabB"
                     /locus_tag="ACHXOL_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR6809731.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2090..2668
                     /locus_tag="ACHXOL_002799"
     CDS             2090..2668
                     /locus_tag="ACHXOL_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR6809732.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHXOL_002800"
     CDS             2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHXOL_002800"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR6809733.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4347..5945
                     /locus_tag="ACHXOL_002801"
     CDS             4347..5945
                     /locus_tag="ACHXOL_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR6809734.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5949..7505)
                     /gene="yoaE"
                     /locus_tag="ACHXOL_002802"
     CDS             complement(5949..7505)
                     /gene="yoaE"
                     /locus_tag="ACHXOL_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR6809735.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7493..7651)
                     /gene="yoaL"
                     /locus_tag="ACHXOL_002803"
     CDS             complement(7493..7651)
                     /gene="yoaL"
                     /locus_tag="ACHXOL_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR6809736.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7968..8939
                     /gene="manX"
                     /locus_tag="ACHXOL_002804"
     CDS             7968..8939
                     /gene="manX"
                     /locus_tag="ACHXOL_002804"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR6809737.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9002..9802
                     /gene="manY"
                     /locus_tag="ACHXOL_002805"
     CDS             9002..9802
                     /gene="manY"
                     /locus_tag="ACHXOL_002805"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR6809738.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9815..10666
                     /gene="manZ"
                     /locus_tag="ACHXOL_002806"
     CDS             9815..10666
                     /gene="manZ"
                     /locus_tag="ACHXOL_002806"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR6809739.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10721..11179
                     /locus_tag="ACHXOL_002807"
     CDS             10721..11179
                     /locus_tag="ACHXOL_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR6809740.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2747 a         2820 c         2826 g         2796 t
ORIGIN      
        1 taaggtcata tatagcctta tcgataacat ggttaatttt aaggataaga atatgcctgc
       61 tgtaatagat aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag
      121 ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat ttaaaagaac tgggagtacc
      181 tgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggtttac
      241 ggaaaaaatg gttggatggg caaaaaaaat ggagtcaggt gaacgctctg tgattaaaaa
      301 tcctgaatac ttttcaacat atatgcagga agaactaaaa gcactggtct gagtaaaatt
      361 tacatcagca taaatggatc aggacgcttt taatcacata taaaaagcgt cctttttctc
      421 acaatcaaat aatcatcggc ttgtataacg aaatcaatac ggattagtgt gttactcgtc
      481 ttcatcctca aaacgcgcca cgattcgctc gccggaatgg ttggcacgca gctcttctgc
      541 caccaacgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc
      601 gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc
      661 ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg
      721 actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga
      781 attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc
      841 cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac
      901 tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc
      961 gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgcccaacgc ataacgaaga
     1021 tttgccattt cagggcggcg cactggggtt gtttggctac gatctgggcc gccgttttga
     1081 gtcactgcca gaaattgcgg aacaagatat cgttctgccg gatatggcag tgggtatcta
     1141 cgattgggcg ctcattgtcg accaccagcg tcatacagtt tctttgctga gtcataatga
     1201 tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc tcgccgcagg aagatttcac
     1261 gcttacttcc gactggcaat ccaatatgac ccgcgagcag tacggcgaaa aatttcgcca
     1321 ggtacaggaa tatctgcaca gcggtgattg ctatcaggta aatctcgccc agcgttttca
     1381 tgcgacctat tctggcgatg aatggcaggc attccttcag cttaatcagg ccaaccgcgc
     1441 gccatttagc gcttttttac gtcttgaaca gggtgcaatt ttaagccttt cgccagagcg
     1501 gtttattctt tgtgataata gtgaaatcca gacccgccct attaaaggca cgctaccacg
     1561 cctgcccgat cctcaggaag atagcaaaca agcagaaaaa ctggcgaact cagcgaaaga
     1621 tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat gatatcggtc gtgttgccgt
     1681 agcaggttcg gtaaaagtac cagagctgtt cgtggtggaa cccttccctg ccgtgcatca
     1741 tctggtcagc accataacgg cacaactacc agaacagtta cacgccagcg atctgctgcg
     1801 cgcagctttt cctggtggct caataaccgg ggctccgaaa gtacgggcta tggaaattat
     1861 cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc agcattggct atttgagctt
     1921 ttgcggcaac atggatacca gcattactat ccgcactctg actgccatta acggacaaat
     1981 atactgctct gcgggcggtg gaattgtcgc cgatagccag gaagaagcgg aatatcagga
     2041 aacttttgat aaagttaata agatattacg ccaactggag aagtaagacg tggaataccg
     2101 tagcctgatg cttgatgatt ttttatcgcg ctttcaactt ttgcgcccgc aaattaaccg
     2161 ggaaacccta aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc gaccgcaacc
     2221 ggggttgttg ctgactcagc gttcaattca tctgcgtaaa cacgctggac aagtggcatt
     2281 ccctggaggt gcagtcgatg acacggacgc atcagttatc gccgccgcgc tgcgcgaagc
     2341 tgaagaagag gtcgctatac cgccttccgc cgttgaagtt atcggcgtgc tgccgcccgt
     2401 cgatagcgta actggctatc aggtaacccc agtggtcggc attatcccgc ccgatctgcc
     2461 gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa atgccgctcg cccaggcatt
     2521 acatctgggt cgttatcacc ctttagatat ctaccgccgt ggcgattcac atcgggtatg
     2581 gctgtcctgg tacgaacagt attttgtatg gggaatgacc gcaggcataa ttcgtgagct
     2641 ggcgctgcaa attggtgtga aaccctgact atacttatct ttacatctac aaaacactac
     2701 ttgagacaat catcgcaata ttagttaaat cgcggttttt gattagttta attcatgtga
     2761 atagttaagc cagtcgccgc gttccctctt acactatgcg ctgttattag ttcgttactg
     2821 gaagtccagt caccttgtca ggagtattat cgtgattagt ctattcgaca tgtttaaggt
     2881 ggggattggt ccctcatctt cccataccgt agggcctatg aaggcaggta aacagttcgt
     2941 cgatgatctg gtcgaaaaag gcttactgga tagcgttact cgcgttgccg tggacgttta
     3001 tggttcactg tcgctgacgg gtaaaggcca ccacaccgat atcgccatta ttatgggtct
     3061 tgcaggtaac gaacctgcca ccgtggatat cgacagtatt cccggtttta ttcgcgacgt
     3121 agaagagcgc gaacgtctgc tgctggcaca gggacggcat gaagtggatt tcccgcgcga
     3181 caacgggatg cgttttcata acggcaacct gccgctgcat gaaaacggta tgcaaatcca
     3241 cgcctataac ggcgatgaag tcgtctacag caaaacttat tattccatcg gcggcggttt
     3301 tatcgtcgat gaagaacact ttggtcagga tgctgccaac gaagtgagcg tgccgtatcc
     3361 gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa accggctatt cgctgtctgg
     3421 tctcgctatg cagaacgaac tggcgctgca cagcaagaaa gagatcgacg agtatttcgc
     3481 gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg atgaacaccg aaggtgtact
     3541 gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgt tagtttccag
     3601 cgataaactg tctaacgatc cgatgaatgt cattgactgg gtaaacatgt ttgcgctggc
     3661 agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca acggtgcctg
     3721 cggtatcgtt ccggcagtgc tggcttacta tgaccacttt attgaatcgg tcagcccgga
     3781 catctatacc cgttacttta tggcagcggg cgcgattggt gcattgtata aaatgaacgc
     3841 ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct gttcaatggc
     3901 tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa caggtttgcg tggcggcgga
     3961 aattggcatg gaacacaacc ttggtttaac ctgcgacccg gttgcagggc aggttcaggt
     4021 gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg cacggatggc
     4081 tctgcgccgc accagtgcac cgcgcgtctc gctggataag gtcatcgaaa cgatgtacga
     4141 aactggtaag gacatgaacg ccaaataccg cgaaacctca cgcggtggtc tggcaatcaa
     4201 agtccagtgt gactaatact tcttactcgc ccatctgcaa cggatgggcg aatttatacc
     4261 cgctttctcg tctgctgtaa tattccccac tacacttcca ctgttgcgtc aggcgtttgt
     4321 cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca acggatcatt aaaacctatc
     4381 gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt tacgctcgct tcgaccctga
     4441 gcgtgcgatt tatttcacag cgtaacttaa atcaacaacg ggtagtacaa ttcgccaatc
     4501 acgctgtaga ggaattagat aaagtactgc ttcccctaca ggcaggtagc gaagtcttgc
     4561 ttccgctgat tggtctgccc tgctctgtcg cccatttgcc attacgtaaa caggcggcaa
     4621 aactccaaac tgtgcgatcc attggcctgg tgcaagacgg aacactctat tgctccagca
     4681 tttttggtta tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct gcaccgcaac
     4741 cacttttacg cctgacgatc gaccgtgccc tgattaaagg cagtccggtt ttgattcaat
     4801 ggacgcctgc agcgggcagt agcaatgctg gggtcatgga gatgattaac atcgacttac
     4861 tgacggcaat gctccttgag ccacaactgc cgcaaatcag tagcgccagc ctgacggtgg
     4921 acaaacggca tttgctctat ggtaatgggc tggtagattc ccttccgcaa cctgaagaca
     4981 atgaaaacta tcaggtttct tcgcaacgct ttccttttac cattaacgtt aatggtccgg
     5041 gggctacggc gctggcatgg cactatcttc caacacaatt accgctggcg gtgctgctaa
     5101 gtttactggt gggctacatc gcctggctgg cgaccgctta ccggatgagc ttttcccgcg
     5161 aaatcaatct gggcctggcg caacatgagt tcgaattgtt ctgtcagcct ttgcttaatg
     5221 cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat ccgcgtcagg
     5281 gctggatttc accggatgtg tttattccta tcgcggaaga acatcattta attgtgccac
     5341 tgacccgcta tgtgatggca gaaaccattc gtcagcgcca tgttttcccg atgagtagtc
     5401 agtttcatgt tggcattaac gtcgcaccca gccattttcg ccgtggtgtg ctgataaaag
     5461 atctcaatca gtactggttt agcgctcacc cgattcagca actgatcctc gaaatcaccg
     5521 aacgcgatgc cttactggat gttgattatc ggattgcccg cgagctacat cgtaaaaacg
     5581 tcaaactggc gattgatgac ttcggcaccg gcaacagttc attttcctgg cttgaaacat
     5641 tacgtcctga catgctgaaa attgataagt catttaccgc agctataggt tctgacgcgg
     5701 ttaactcgac ggtgaccgat atcatcatcg cgctggggca aagactgaat attgaactgg
     5761 tggcggaggg cgtggaaacg caagaacagg cgaagtattt gcgccgtcat ggcgtgcata
     5821 ttttgcaagg gtatttgtac gcacagccga tgccgctacg tgattttccc aaatggctgg
     5881 cgggcagcca accgccgccc gcccggcata atggacatat cacgcccatt atgccgttac
     5941 gttaatgctt actcatcttc atcgtgcgcc ggttgctctt taacaatgcg aaccagatca
     6001 acacgataat cattggcttc aatgatggtg atatgcagtg gccctacatc aatcacatcg
     6061 cccacacggg gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt cgcgatatca
     6121 tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca aggcatgcaa atctgtaccg
     6181 ccttttacca gccagccgtc accatcagta atgatttccg gcgtttcgtc agcgtccggg
     6241 aattcacccg caatggcttc cagcacatcc agcggcgtga ccagaccttg taccacacca
     6301 aactcgttgg tcacgataac aaagctcccg cgagcacgac gcagcacgcc caacaggttg
     6361 atcggatcga gggtttccgg gacgataatc gccggagacg ccgaagcaat cgccgccaca
     6421 tcaacgccct cttccagcgc caccagcagt tctttagcac gtacaatacc gatgatttca
     6481 tccagttcac cgcgacatac cgggaacaga ctgtgcggtg aagagagcag ttgctcgcgg
     6541 atttcatcga ccccgagatt agcgtcaacc cagcttattt caccgcgcgg cgtcatgatc
     6601 ccgcgcagag aacgcgacgc aagcgtcagt acgccgttaa tcatgtaacg ttcttcttcg
     6661 gcaaatgcac cttccgggat cggcatcggc atcgggttat cggcatcgtg ctgaacattg
     6721 gcctgacgtt tcccgcccat caaacgcagg atggcatcgg cagtacgcgc tcgcagcggc
     6781 aaagtcgact ggtggcgaat aaagttgcga cgcgcaatct ggttaaacac ttcgatgatg
     6841 atcgagaagc caatcgcggc atacaggtaa cctttcggaa tgtggaaacc gaaaccttct
     6901 gccaccagac tcagaccaat cattaacagg aagctcagac agagcaccac caccgtgggg
     6961 tgctggttaa cgaatcgcgt cagcggtttg gatgccagca acataaccgc catcgcaatc
     7021 actaccgccg ccatcatcac cggcagatgg ttaaccatcc ctactgcagt aattaccgca
     7081 tccaacgaga agacggcgtc aaggatgaca atctgtgtga cgaccaccca gaaactggcg
     7141 tagcctttac cgtggccgga atcatgatcg cggttttcca gccgttcatg cagttcggtt
     7201 gttgctttga acagcaagaa tatccccccg aacaacataa tcaggtcgcg tccggagaag
     7261 gagaaatcca tgacggtaaa tagcggtttg gtcagcgtga ccatccatga aatcagcgac
     7321 agcagcccca gacgcataat cagcgccagt gataacccca gcaaacgcgc tttatcgcgt
     7381 tgttttggcg gcagtttgtc agcaagaatg gcgatgaaga ccaggttatc gatacccagc
     7441 acaatttcga gaacaacaag cgtgagtagc cccgcccaaa ttgaggggtc cattaagaat
     7501 tccatgacaa gctcctgctt aaggaatagc tattcgacgc cagaaataat gcaggcgtaa
     7561 cgacaaaatg caaacgaaag gtgcggcata gagtgccaga aaggcaggcg ttaaaaggcc
     7621 tgatgctgaa atgacgtcgg tgacgatcca tactgcgggc tactgcccta tactccatgg
     7681 ttgttaaacg ggagttaaac atatcagaga cgcctctgat ttggcaaaga tttaccttcc
     7741 tttgcaaacg aatgtgacaa ggatatttta cctttcgaaa tttctgctaa tcgaaagtta
     7801 aattacggat cttcatcaca taaaataatt ttttcgatat ctaaaataaa tcgcgaaacg
     7861 caggggtttt tggttgtagc ccttatctga atcgattcga ttgtggacga cgattcaaaa
     7921 atacatctgg cacgttgagg tgttaacgat aataaaggag gtagcaagtg accattgcta
     7981 ttgttatagg cacacatggt tgggctgcag agcagttgct taaaacggca gaaatgctgt
     8041 taggcgagca ggaaaacgtc ggctggatcg atttcgttcc aggtgaaaat gccgaaacgc
     8101 tgattgaaaa gtacaacgct cagttggcaa aactcgacac cactaaaggc gtgctgtttc
     8161 tcgttgatac atggggaggc agcccgttca atgctgccag ccgcattgtc gtcgacaaag
     8221 agcattatga agtcattgca ggcgttaaca ttccaatgct cgtggaaacg ttaatggctc
     8281 gtgatgatga cccaagcttt gatgaactgg ttgcgctggc agtagaaaca ggccgtgaag
     8341 gcgtgaaagc actgaaagcc aaaccggttg aaaaagccgc gccagcaccc gctgccgcag
     8401 caccaaaagc ggctccaact ccggcaaaac caatggggcc aaacgactac atggttattg
     8461 gccttgcgcg tatcgacgac cgtctgattc acggtcaggt cgccacccgc tggaccaaag
     8521 aaaccaatgt ctcccgtatt attgttgtta gtgatgaagt ggctgcggat accgttcgta
     8581 agacactgct cacccaggtt gcacctccgg gcgtaacagc acacgtagtt gatgttgcca
     8641 aaatgattcg cgtctacaac aacccgaaat atgctggcga acgcgtaatg ctgttattta
     8701 ccaacccaac agatgtagag cgtctcgttg aaggcggcgt gaaaatcacc tctgttaacg
     8761 tcggtggtat ggcattccgt cagggtaaaa cccaggtgaa taacgcggtt tcggttgatg
     8821 aaaaagatat tgaggcgttc aagaaactga atgcgcgcgg tattgagctg gaagtccgta
     8881 aggtttccac cgatccgaaa ctgaaaatga tggatctgat cagcaaaatc gataagtaac
     8941 gtattgtgtt gattatcact cagttttcac acttaagtct tacgtaaaca ggagaagtac
     9001 aatggagatt accactcttc aaattgtgct ggtatttatc gtagcctgta tcgcaggtat
     9061 gggatcaatc ctcgatgaat ttcagtttca ccgtccgcta atcgcgtgta ccctggtggg
     9121 tatcgttctt ggggatatga aaaccggtat tattatcggt ggtacgctgg aaatgatcgc
     9181 gctgggctgg atgaacatcg gtgctgcagt tgcgcctgac gccgctctgg cttctatcat
     9241 ttctaccatt ctggttatcg caggtcatca gagcattggt gcaggtatcg cactggcaat
     9301 ccctctggcc gctgcgggcc aggtactgac catcatcgtt cgtactatta ccgttgcttt
     9361 ccagcacgct gcggataagg ctgctgataa cggcaacctg acagcgattt cctggatcca
     9421 cgtttcttct ctgttcctgc aagcaatgcg tgtggctatt ccggccgtca tcgttgcgct
     9481 gtctgttggt accagcgaag tacagaacat gctgaatgcg attccggaag tggtgaccaa
     9541 tggtctgaat atcgccggtg gcatgatcgt ggtggttggt tatgcgatgg ttatcaacat
     9601 gatgcgtgct ggctacctga tgccgttctt ctacctcggc ttcgtaaccg cagcattcac
     9661 caactttaac ctggttgctc tgggtgtgat tggtactgtt atggcagtgc tctacatcca
     9721 acttagcccg aaatacaacc gcgtagccgg tgcgcctgct caggcagctg gtaacaacga
     9781 tctcgataac gaactggact aacaggtgag cgaaatggtt gatacaactc aaactaccac
     9841 cgagaaaaaa ctcactcaaa gtgatattcg tggcgtcttc ctgcgttcta acctcttcca
     9901 gggttcatgg aacttcgaac gtatgcaggc actgggtttc tgcttctcta tggtaccggc
     9961 aattcgtcgc ctctaccctg agaacaacga agctcgtaaa caagctattc gccgtcacct
    10021 ggagttcttt aacacccagc cgttcgtggc tgcgccgatt ctcggcgtaa ccctggcgct
    10081 ggaagaacag cgtgctaatg gcgcagagat cgacgacggt gctatcaacg gtatcaaagt
    10141 cggtttgatg gggccgctgg ctggtgtagg cgacccgatc ttctggggaa ccgtacgtcc
    10201 ggtatttgca gcactgggtg ccggtatcgc gatgagcggc agcctgttag gtccgctgct
    10261 gttcttcatc ctgtttaacc tggtgcgtct ggcaacccgt tactacggcg tagcgtatgg
    10321 ttactccaaa ggtatcgata tcgttaaaga tatgggtggt ggcttcctgc aaaaactgac
    10381 ggaaggggcg tctatcctcg gcctgtttgt catgggggca ttggttaaca agtggacaca
    10441 tgtcaacatc ccgctggttg tctctcgcat tactgaccag acgggcaaag aacacgttac
    10501 tactgtccag actattctgg accagttaat gccaggcctg gtaccactgc tgctgacctt
    10561 tgcttgtatg tggctactgc gcaaaaaagt taacccgctg tggatcatcg ttggcttctt
    10621 cgtcatcggt atcgctggtt acgcttgcgg cctgctggga ctgtaagact gttgtacact
    10681 accggggcct tttggccccg tttttttatc tggaggatta atgacaatca cggacctggt
    10741 actgattctt ttcatcgccg cactcctggc cttcgcgatc tacgatcagt tcatcatgcc
    10801 ccgccgtaac ggccccaccc tgctggcaat tcctttgctc cggcgtggtc gcatcgatag
    10861 cgttatcttc gtcggattga ttgtcattct tatctataac aacgtcacga atcatggtgc
    10921 gttaataacg acatggttat taagcgcact ggctctgatg ggtttttata tattctggat
    10981 ccgcgttccg aagatcatct ttaaacaaaa aggttttttc ttcgccaatg tctggattga
    11041 atatagccga atcaaagcga tgaacttgtc ggaagatggc gtgttggtga tgcaattaga
    11101 acagcgtcgg ctgttaatcc gcgttcgaaa tatcgacgat ctggaaaaaa tttataagct
    11161 tctcgtttca actcaataag ttatgaatt
//
LOCUS       DAZRUF010000135        11175 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZRUF010000135 DAZRUF010000000
VERSION     DAZRUF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11175
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.135"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHXOL_002808"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHXOL_002808"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR6809741.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHXOL_002809"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHXOL_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR6809742.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /locus_tag="ACHXOL_002810"
     CDS             complement(3743..4162)
                     /locus_tag="ACHXOL_002810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR6809743.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHXOL_002811"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHXOL_002811"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR6809744.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHXOL_002812"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHXOL_002812"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR6809745.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHXOL_002813"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHXOL_002813"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR6809746.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHXOL_002814"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHXOL_002814"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR6809747.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHXOL_002815"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHXOL_002815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR6809748.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHXOL_002816"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHXOL_002816"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR6809749.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHXOL_002817"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHXOL_002817"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR6809750.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHXOL_002818"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHXOL_002818"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR6809751.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2682 a         3034 c         2754 g         2705 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attggccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt tgttcggtgc
      301 aacgacgata accggcatat cggcatcaat aagcgccagc ggaccgtgtt tcagttcacc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaagccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatcca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgcccg actttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaaa
     2401 cgagcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaagtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaagatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtaag cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagta gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctca aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    10441 acgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactagtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agtatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 acagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat acaaaagagt gaacgtggcg
    11161 ttaaatgtaa ccagt
//
LOCUS       DAZRUF010000136        11076 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZRUF010000136 DAZRUF010000000
VERSION     DAZRUF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.136"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            305..835
                     /gene="ppa"
                     /locus_tag="ACHXOL_002819"
     CDS             305..835
                     /gene="ppa"
                     /locus_tag="ACHXOL_002819"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR6809752.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(1214..1555)
                     /locus_tag="ACHXOL_002820"
     CDS             complement(1214..1555)
                     /locus_tag="ACHXOL_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR6809753.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(1558..5337)
                     /gene="tamB"
                     /locus_tag="ACHXOL_002821"
     CDS             complement(1558..5337)
                     /gene="tamB"
                     /locus_tag="ACHXOL_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR6809754.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(5334..7067)
                     /gene="tamA"
                     /locus_tag="ACHXOL_002822"
     CDS             complement(5334..7067)
                     /gene="tamA"
                     /locus_tag="ACHXOL_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR6809755.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            7273..7911
                     /gene="msrA"
                     /locus_tag="ACHXOL_002823"
     CDS             7273..7911
                     /gene="msrA"
                     /locus_tag="ACHXOL_002823"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR6809756.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            8234..9577
                     /locus_tag="ACHXOL_002824"
     CDS             8234..9577
                     /locus_tag="ACHXOL_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR6809757.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(9657..9863)
                     /locus_tag="ACHXOL_002825"
     CDS             complement(9657..9863)
                     /locus_tag="ACHXOL_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR6809758.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            10188..10742
                     /locus_tag="ACHXOL_002826"
     CDS             10188..10742
                     /locus_tag="ACHXOL_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR6809759.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(10832..>11076)
                     /gene="ytfI"
                     /locus_tag="ACHXOL_002827"
     CDS             complement(10832..>11076)
                     /gene="ytfI"
                     /locus_tag="ACHXOL_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR6809760.1"
                     /translation="YGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAI
                     VSANLAADSNITKEKICEKYKSTIEAFKKEQLALPV"
BASE COUNT         2630 a         2991 c         2682 g         2773 t
ORIGIN      
        1 tgtcctcgtc ggggtaatta ttattggtga aaagatgttc gcgaaaaaac tatagacaat
       61 tcgttatgta acggattgcg ttacatcaca cttcaaaaaa gtaaataaaa cgttaatcac
      121 aagtttgtaa tcgctttcat ctcactatga aaaatgcggc tacggttatg gattttcctg
      181 ctctgtatac cgtcttaaaa ctggcgaaaa aggaaaatga agacgaaaac aagcaaagac
      241 attcggcgcg agttggctat aatactcggc acttgtttgc cacatatttt taaaggaaac
      301 agacatgagc ttactcaacg tccctgcggg taaagatctg ccggaagaca tctacgttgt
      361 tattgagatc ccggctaacg cagatccgat caaatacgaa atcgacaaag agagcggcgc
      421 actgttcgtt gaccgcttca tgtccaccgc gatgttctat ccgtgcaact acggttacat
      481 caaccacacc ctgtctctgg acggtgaccc ggttgacgta ctggtcccga ctccgtaccc
      541 gctgcagcct ggttctgtga tccgttgccg tccggttggc gttctgaaaa tgaccgacga
      601 agccggtgaa gatgcgaaac tggttgcggt tccgcacagc aagctgagca aagaatacga
      661 tcacattaaa gacgtaaacg atctgcctga actgctgaaa gcgcaaatcg ctcacttctt
      721 cgagcactac aaagacctcg aaaaaggcaa gtgggtgaaa gttgaaggtt gggaaaacgc
      781 agaagccgct aaagctgaaa tcgttgcttc cttcgagcgc gcaaagaata aataagttct
      841 tctagcgcaa taaccctgaa cgccgggctt cggttagtaa gggtttttta tgcccgcgat
      901 aaataaactc tctattccac catcattatt ctcagcggtt gcaaggcttg aacggtaaga
      961 acaagcaaac ccgaccacca ttttgctgtt catagccact tgctggaagt tagccgacct
     1021 cactcatact caccgttcaa agcgtgcccc cgttttaccc ctgcacattt gtgaacgcca
     1081 gcagagtttt ttatgcgatt gcagatatgg aaagcggctc ggagagaaaa gcaaaagatg
     1141 gaaaagttac aaaaaaaaat cgcgagatac atcgcataaa acaacgccac ccgaaggtgg
     1201 cgtgctttta ttattactta tccctgtcta accagtcgcc gctttcaatt agctttaatc
     1261 catcgacggg tcgttgataa acgtacatcc atgcactccc gtacggcgtc tgaatcaact
     1321 ggcgcgcgta ttcaccgccc ctggtgcgca aggcatcaag ttcggccagc gtggcgttgt
     1381 caatacgata aacttcaccg tgtaccgttc cgttccccgg aactgcgcct ggatagtggc
     1441 ccaggctata caactggtag ttatcgatac tgaaatcgcc cagtaactgg gcattggtca
     1501 tccagtgact gttgccttgt ttgtggcgta aactgccgta gacaaatatt cgcattgcta
     1561 aaactcgaac tgatagagca aatccagtgc ctggtctaca ccagacacgg cttccagata
     1621 tagcttaggc atcaggcgat aacgtaacgt gagtgttgct atagagtcaa atatacccac
     1681 gccgtatttc acttgcagac ctggcaatac atagccgctg accactacct gggaggagtc
     1741 gcctactccc tgggtgtcga gcgctaaatt gcttacgcca aacgtctcgc cgattttacc
     1801 cacaatctgg ccactttgcg caacccccag accaatcagc atcgaggtca ttgccgcact
     1861 gtcgctctga tcgctctcca gcccttgtcc acgtagcaaa taagacaatg cagcttgttg
     1921 cgacatcgcc gggtcagaga agatctccgc tttcggttcg tccgccagac cagtgacgcg
     1981 aactccggca attacgtcgt cttctgtagc atccgggtta cgaatagctt caatattaag
     2041 atacggttga tccggcggac cagagaacag taactcaccc ttacgcacaa tcagatcctg
     2101 accataggca tggaagcgcc cttcagggat gttgatctgc ccgttcaggc ccagcccttg
     2161 tttgtcctgt acgacgttaa gatcgcccgt cagccgcgct ttcaggccaa aggcgtcaat
     2221 gcgcacattg ttgccaacgt ggacaatcag gttactgtta atcggaatcg acgccgtttt
     2281 cggctcttcc ggttgcaggt tatcgttaag catcaccaca tcgctggaga cgcctactgc
     2341 gctttccggc agatcgtgca ccacgatgcg cgcccacggg acatccacgc gaccatcgag
     2401 ggtaaacagg tttggtgtag cctcgaatac aacatctggc gatacatcca ttcgtaccat
     2461 cggcggcacg gtgatccgca ctttactgcc tttcgccgtt actcgtgccc gccagttttc
     2521 aatttggctc cagtcggcat caccgttcag gtagatttca ccctgctggg tccgtactgt
     2581 accggcaagc gtcgagcgca taccgttaaa gttgaccgca agctggctcg gctgcatatc
     2641 aaacggcata aagttgccgt cgatatccac acccgtaacc tgaagctgac caaacaactg
     2701 cgggctttgc acatcaccac ccagacgcaa gttggcactc accatccccg ctgctttttc
     2761 cccacgggta aagatggggt ttatcatcgc aaggttgaag ttacggatat tgacgttgcc
     2821 accaagatta cggcggcctt gcggatcggt cacctgcacc tgtccatcaa actggccgtt
     2881 attggtcagg cggatggtcc agcccaattc ggcacggttg ttacgcaatt ccgccgtcag
     2941 attcagtgtc tgaaacgcca ccggcagcgc cgcatcgttg acggtttgcg ttacctgcac
     3001 gttacgcccc gaaagggtga tactgccctg cggcagcccc tctttcgtgg tgtcccaggc
     3061 aacatccgct ttacccgtga agataccgct ggcctgagtg gtttctggca taaacggttt
     3121 cagcatggcg aggtcgaagc ggttgagatt caccaccgca cgcccttcgg cccccgcatc
     3181 gatagtttgc ggcacgcaca gttccgcatt cgggttaagc cagcagtgtg gcccgatgct
     3241 gattttttgc tccttattgc ggtaatccag cgcaatatcg cgggtcagcg accacgggcc
     3301 gaccggcgtc tggaaacggg tattgctaag agttcccttc cagcgttctt ctttgcgatc
     3361 aaaacttcct gccagattaa gctgcccgga gacaggctcg ccctgaatcc gcaactgtag
     3421 ctcgtgctgc ttttcgctgc ctttggcatt cagggtgacg aggttgatat ttacatccgg
     3481 ctgcgaaatt tgctcaacgc gtacgtcgag tttcccggcg atctgatcgg tggatttaat
     3541 gtcgccttcc acgcgaacct gcgccacgga aagttcctgc cagcgcaggc cgcgcgcggt
     3601 gatatctgcc agtagttgtg gcgcttccac cgtgccgcgt actttcacca gccctttcgc
     3661 tgtaccgcca agccccggca gcgcgttatc cagccccggc gcgttgatgg tggcatcaag
     3721 attgagatct tttaccccca gctcgccttt cacttcggca ctgtttggcc cgagttccag
     3781 atgaagccca gggatcatcc actgcatata actgttgcct ttcagcgtgc cgtcaacgtt
     3841 cactttgttc tgtttgacgt taccggtcag cttcagttct ggcacctcca tctgccaggt
     3901 gccgccgtac aggctaccgc gggttttaat caagccattg agtttcgacg gccactccgg
     3961 gaactctttg gcggtgttaa tgccattaag cgttagctca ccgcgccaac taatggcctg
     4021 ctgccagtcg agcaacgcct tgagttcagt tttcccttcc agcgccgcga cggtgagttt
     4081 gtcgagattg acctgctgtt cattaccttt ggcatcaagg gtaatggtgg ctggcgggat
     4141 ctcctgtccc ttcactgccg tacgcataga gagcgtgtaa tcggtcattt tgccggtaag
     4201 tttcagtttc agatcatccg cctgatactg cttctcacca gtgaacggcc agtaaagctg
     4261 tttgctgttc acttccacgt tgagtggcaa tccggcttcc gccagtcgcg tctgggcgcg
     4321 taaatccata tccaccggac cggaaaggtt aacgcctatc tccagctgtt cgcgcagcgc
     4381 gccgcccact ttcagcttca ccttttcacc tttcaacggc tccacgttca gtgtgctgtt
     4441 gaggatgata tccaccggcc agttgtctga cagctgcgcc gtgccgctgg cgttgacgat
     4501 cccctggttg gaatcgatat ccagggcgtc cagtttagta ttgccgtcaa tgctgctcac
     4561 tttcagcagc atggtgcgca cggtgatgtc cgtgtcgccc gtcacgcgca gctgctcgcc
     4621 cttaaactct tcaatgttca ggttaagcgg caaatgcacg tcggtcattt ccggcaatac
     4681 cgggcgagaa aagagatctt tcagcgtttc gccgagcggc ttttcatccg gctgcggatt
     4741 ttcaatcttc ggttcgacga cctcttcctg cgccacttcc gccactttcg gcagagcaat
     4801 cagcaggcct ttcagcgacg tcggtttcag ggtcagggtt ttctcctgcc agttcaggcc
     4861 ggaggtgaag tccatcaccg acaccgtggt gtcatcaatc ttgatgttga cgttatccag
     4921 cgccacccgt gtcagggtga tgggatacgg cgtggagaga tccagcggac cgctatcttc
     4981 ctcttcttca acctgttcag aaggaggcat ttttttactg tcgatgttga cctgaatgtc
     5041 tttcagcgcc aggtcattaa tacaaacgct actgttccac aggcactcaa gaccgaccgc
     5101 cagatgcaga ttacccgctt ttaccgccac gcctggctgc tcataacgaa cgtcagacaa
     5161 ggtgagatcg cgccagccgc cggtgacgtt gccaatatcc agtcctggca cccagcgatc
     5221 cgccgcttta aatacaagat gcaggccgct ggtggtgccc accagaaacg ccaccgatcc
     5281 cagcaacagt aagataacga taaccacgcc gaggctgatt tttttccata aactcataat
     5341 tctggcccca gaccgatgta aaactgtaac ccgtgttcgt ctttatccgc gaccggtacg
     5401 gcaaaatcga gtttgattgg cccgaccggc gattcccagc gtacgccgcc cccggtaccg
     5461 gttttaaagt cgctgcggcg aatatcgctt accgcttcgc cactatcgac aaacaccgcg
     5521 ccccaccatt ttccggtcac gttgtactgg tattccagcg atccggttat cagcttcgag
     5581 gcccctttca ggtcaccgtt ggcgtatttc ggggcgatag atttatattt gtagccgcga
     5641 atactacgat cgcctccggc gaagaaacgc agatccggcg gtactttgtc gaaatcaccg
     5701 gtttcaatcc agcccagcgt gccgcgtgtc acaaaacgat ggcgatcgta cagtgtgcgg
     5761 atccagacgt tctgcgcctg gaaaacggag aaatcgacat ctgaacccca ggccgtgttg
     5821 gagtagtcga tagagtagcg ttgcgagtcg ccccaggttg gcatcaggcc accacgagaa
     5881 cgcgtgcggc taatcatcac cccaggataa aacagcatcg tggtgttggt aatttcaccc
     5941 tgggtaaagt ggtcgaggct ccagcgcagg ttaatggcac gctgccagtc gctggagaga
     6001 tcccagtagc gagaagccac cagcgtagtg gagtcagatt cggtatcgtt caggtcagtg
     6061 cgcttaaaac cgccctgcac caaataatat tgttccagtg gattcttcag cagcggcatt
     6121 ttatagctga agtcgaggat ctgttccggc gcggaaatac tggtactggt ggtcagactg
     6181 tgaccataag agttcatcca cggctttttc cacgtcgctt tcacgcgcgg tcccacgtcc
     6241 gtagagtaac cgaccccggt ttcgatggtg ttttctgttc gcggcgaaac cacgcccgtc
     6301 aatggtaata ctttcgtttc gcgcgcttta tcaaattgcg gtgccactac caccgaatta
     6361 aaccagccgg tagcagaaag tcggcggttc agttccgcca gatctttcga ttcatactca
     6421 tcgccctctt taaacggcac cagattttgc aggtattcat cgcggatttg tgatccttca
     6481 aaggtcacat gcccaaagcg gtaacgttcg ccactgttat aatcaatatc ccagaaggct
     6541 ttatgcaggc cgagcgcaat gcccagctgc gctttggtaa attcgctatc gaaataacct
     6601 ttacgcaacg caatactggt taaggacttt ttgaaatttt cataatcgcc ctggttcagt
     6661 accgtaccaa tagccgggcg agtatcgagc aatttcaaat agtctttatc ggtccgcgcg
     6721 ccgccgcgca ataccacatc ggtgccgcca attaacaccg gtacacctgg cgtgactttg
     6781 gcgatcaata cctgccgccc tttctttggc ggtggacgga gatcaaattc aatggtcggc
     6841 tggtaataac ccagcgcttt cagaccttca cggatggcat catcgacgcg tgcgcgaaag
     6901 cgacggtctg gcgtcacttc atcactttca atcgtagaaa gctgcgcacg aacgttcttt
     6961 tccagctgtc ccgataaccc ctcgacctgt agacggacgt tcgcggcgac ggcagatccg
     7021 cttaagcaga gtaagcttac acagcataac tgtcggatat agcgcacatt ttctcctgaa
     7081 tatccttttt tcctacccct ggaacgccgt taaacggctt aacaaaaatc cagtaatatg
     7141 gattaaaaag cagactaaac cccaaatatt tcttatgttt tactttagac ctattcacgg
     7201 tggttattgt gtgcaaatac gcctcttgtt acaaccttaa ccccaatgac cgattttcgg
     7261 gagagcgaca ccatgagttt atttgataaa aagcatctgg tttcccccgc cgatgccctg
     7321 cctggacgta acaccccgat gcccgtagcc acgctgcatg cggtcaacgg tcactcaatg
     7381 accaatgtac ctgacggaat ggagattgcc atttttgcga tgggttgttt ctggggtgtg
     7441 gagcgtctgt tctggcagtt acccggcgtt tacagcaccg ccgcaggcta taccggcggc
     7501 tatacgccaa atccgactta tcgggaagtg tgctccggtg atacgggtca tgccgaagcg
     7561 gtacgcattg tttacgatcc ttccgtcatc agctatgagc agttgctaca ggtattttgg
     7621 gagaatcacg atcccgccca gggaatgcgt cagggcaatg accacggcac gcagtatcgt
     7681 tcagcgattt atccgctgac cccagaacag gatgccgcag ctcgcgccag tctggaacgt
     7741 tttcaggcgg cgatgcttgc cgccgatgac gatcgtcaca tcaccacgga aatcgctaac
     7801 gccacaccgt tttattatgc cgaagatgac caccagcaat atctgcataa aaacccatat
     7861 ggttactgtg gaattggcgg aattggcgtc tgtctgccgc cggaagcata gcgttacggg
     7921 tacaaatgta gtttgttgat aaagtgctct ttgtctatgc ctgatgcggc ttaaacgact
     7981 taccaggcct acaaaatcgt ccaaattcaa tatattgcaa ggactgcgta ggcctgatcg
     8041 gcatagcgca tcaggcagta ttgcatttat cagcagtctg aattttaacc ctctggcgac
     8101 tttacagtac cttacgctat actagccact gaaaatgtcg gttcactttc ttcgaatcga
     8161 ctttcaatgt gtatttcaca caaattaatc aactttccct tccgaggatc tggcctgaaa
     8221 ggtcggataa gatatgttaa acagtatttt agtcatactc tgcttgatcg ctgtaagtgc
     8281 gttcttctcg atgtccgaga tctcgcttgc cgcctcacgc aaaatcaaac ttaaactgct
     8341 ggctgatgaa ggcaatataa atgcccaacg cgttctgaat atgcaggaaa atcccggcat
     8401 gttctttacc gtggtccaaa tcggtctgaa cgcagtggcg attctcggcg gtatcgtcgg
     8461 tgatgcggca ttttctccag cttttcacag cctgttctcc cgctatatgt cggcagagct
     8521 ctctgagcaa ctgagcttta ttctctcttt ctcgttagtg actggcatgt ttatcctgtt
     8581 tgcggattta accccgaaac gcatcggtat gattgcgcca gaagcggtgg ctttgcgtat
     8641 catcaacccg atgcgcttct gcctgtacgt ttgcacccca ctggtgtggt tcttcaacgg
     8701 cctggcgaac atgatcttcc gtattttcaa actgccaatg gtacgtaaag atgacatcac
     8761 ttctgatgac atctacgcgg tagtggaagc cggtgcgctg gcgggcgtgt tacgtaaaca
     8821 ggaacacgag ctgattgaaa acgtctttga gctggaatcc cgtaccgttc cgtcttcaat
     8881 gacaccgcgt gaaaacgtga tttggtttga tctccacgaa gatgagcaaa gcctgaagaa
     8941 taaggtggcg gaacatccgc actctaagtt cctcgtctgt aatgaagata ttgaccacat
     9001 catcggttat gtcgattcta aagacctgct gaaccgcgtg ctggctaacc aaagcctggc
     9061 actgaacagc ggcgtacaaa ttcgcaacac gctgattgtg ccggatacgt taaccctttc
     9121 agaggcgttg gaaagtttta aaaccgcagg tgaagacttc gcggtgatca tgaacgagta
     9181 cgcgctggtg gtggggatca tcaccctcaa cgacgtgatg accacgctga tgggcgatct
     9241 ggtcggtcag gggctggaag agcagattgt cgcccgtgat gagaactcat ggctgattga
     9301 cggcggcact ccgattgacg acgtcatgcg cgtgctggat attgacgaat tcccgcagtc
     9361 gggcaactac gaaaccatcg gcggctttat gatgtttatg ctgcgtaaga tcccgaaacg
     9421 caccgattcg gtgaaattcg ccggctacaa atttgaagtg gtggatatcg ataactaccg
     9481 catcgaccag ctgctggtga cccggatcga cagcaaggcc accgcccttt cgccaaaact
     9541 gcctgacgct aaagataaag aagaaagcgt cgcgtaaccc cccagaaaca tcaacggctc
     9601 ctgaatcagg agccgtctta ttactgcata gcacttttta ctgcatagca ctttggttaa
     9661 gccatctctg tttgcagacg cataacctga cggttaactt cggacatcac tgacaaatgc
     9721 agcttatcct tcacttttgg gataagaatc ttacccttat caaattcaaa agcgccaacg
     9781 tccttgatgt ataaccgtcc acggaacagg atctttacgt acttcgccac ctgaagtggg
     9841 ttgtaccgtt ggaaaatttt cattgttatc tcctgctgag tattacgcct gtgcggtgcc
     9901 acaatcggcc caactattat gaggcgcaaa ttttaatgct tagtgactat agactatccg
     9961 ggcaatgttt ccaccgcgta taactttttt tacctaaagg ttacaattat tcagaattat
    10021 ctttttaccg aagcgcggtc ttcagtataa gcattcattt ttcatatgat ttgtgcgctt
    10081 gaccgcaaac tggcatcaca cttgcgggaa attcgataaa tagcacatat gattaaaact
    10141 cagacccaag tggtcggatc acctgcatat cataagaagg aaacaccatg accctacgca
    10201 agattctggc actcacctgc ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa
    10261 ccggtcagcg agtgccgccg attggcatta ccgatcgggg cgagttggtg cttgataaag
    10321 atcagtttag ctacaaaacc tggaacagcg cgcagttagt gggaaaagtg cgagtactgc
    10381 aacatattgc tggtcgcacc tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta
    10441 aatcagcgaa gttaccgcac gatcgttacc agaccaccac tattgttaac accgacgacg
    10501 cgattccggg ttcagggatg tttgtgcgca gcagtctgga gagtaataaa aagctttatc
    10561 cctggtcgca gtttattgtc gatagcaatg gcgtcgcgcg cggtgcctgg cagctggatg
    10621 aagaaagttc cgctgtagtg gtgctggata aggatggtcg cgtgcaatgg gccaaagacg
    10681 gggcgctcac tcaggaagag gtgcagcaag tgatggacct gctgcaaaag ttgctcaaat
    10741 aatggcaaca acatagattc tgcaaaaata attttcccct atcgttatct atgggatagg
    10801 ggaattaagt ctctgagttc ttacgccgaa ctcatactgg taacgctagt tgctcttttt
    10861 taaacgcctc aatggtcgac ttatatttct cgcaaatctt ttctttggta atattactat
    10921 ctgcggccag attcgcactt actatcgcat ccacttgttc ggctaattcc acttgttggc
    10981 aacctgcatt aaccagaaaa ttataaataa gggagtaatc ttcatcctcc aaaacaacag
    11041 aggaaccatt acgaaaacgt tcatcccctc cataga
//
LOCUS       DAZRUF010000137        10891 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZRUF010000137 DAZRUF010000000
VERSION     DAZRUF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.137"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            179..826
                     /gene="ychE"
                     /locus_tag="ACHXOL_002828"
     CDS             179..826
                     /gene="ychE"
                     /locus_tag="ACHXOL_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR6809761.1"
                     /translation="MIQTFLDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1564..3195
                     /gene="oppA"
                     /locus_tag="ACHXOL_002829"
     CDS             1564..3195
                     /gene="oppA"
                     /locus_tag="ACHXOL_002829"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR6809762.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            3281..4201
                     /gene="oppB"
                     /locus_tag="ACHXOL_002830"
     CDS             3281..4201
                     /gene="oppB"
                     /locus_tag="ACHXOL_002830"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR6809763.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            4216..5124
                     /gene="oppC"
                     /locus_tag="ACHXOL_002831"
     CDS             4216..5124
                     /gene="oppC"
                     /locus_tag="ACHXOL_002831"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR6809764.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            5136..6149
                     /gene="oppD"
                     /locus_tag="ACHXOL_002832"
     CDS             5136..6149
                     /gene="oppD"
                     /locus_tag="ACHXOL_002832"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR6809765.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            6146..7150
                     /gene="oppF"
                     /locus_tag="ACHXOL_002833"
     CDS             6146..7150
                     /gene="oppF"
                     /locus_tag="ACHXOL_002833"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR6809766.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(7203..7532)
                     /locus_tag="ACHXOL_002834"
     CDS             complement(7203..7532)
                     /locus_tag="ACHXOL_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR6809767.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(7567..9027)
                     /gene="cls"
                     /locus_tag="ACHXOL_002835"
     CDS             complement(7567..9027)
                     /gene="cls"
                     /locus_tag="ACHXOL_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR6809768.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            9170..9343
                     /locus_tag="ACHXOL_002836"
     CDS             9170..9343
                     /locus_tag="ACHXOL_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR6809769.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(9398..10651)
                     /gene="kch"
                     /locus_tag="ACHXOL_002837"
     CDS             complement(9398..10651)
                     /gene="kch"
                     /locus_tag="ACHXOL_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR6809770.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
BASE COUNT         2841 a         2503 c         2682 g         2865 t
ORIGIN      
        1 ttgtagtatg atttggtggc tacattagca tgttttgcac aactagataa caataacgaa
       61 tgatagcaat tttaagtagt taggaggtga aaaatgctgt caaaaggcgt attgtcagcg
      121 cgtcttttca accttaattt atggctaaca ttatccggct tttgcttcgg agctaaccgt
      181 gattcagacc tttttggatt ttcccgttta cttcaaattt ttcatcgggt tatttgcgct
      241 ggtcaacccg gtagggatta ttcccgtctt tatcagcatg accagttatc agacagcggc
      301 agcgcgaaac aaaactaacc ttacagccaa cctgtctgtg gccattatct tgtggatctc
      361 gctttttctc ggcgacacga ttctgcaact ttttggtata tcaattgatt cgttccgtat
      421 cgcagggggc atcctggtgg tgacgatagc gatgtcgatg atcagcggca agcttggcga
      481 ggataaacag aacaagcaag aaaaatcaga aaccgcggta cgtgaaagca ttggtgtggt
      541 gccattggcg ttgccgttga tggcggggcc aggggcgatc agttctacca tcgtctgggg
      601 tacgcgttat cacagcatta gctatctgtt tggtttcttt gtggctattg cattgttcgc
      661 tttatgttgc tggggattgt tccgcatggc accgtggctg gtacgggttt tacgccagac
      721 cggcatcaac gtgattacgc gtattatggg gctattgctg atggcattgg ggattgaatt
      781 tatcgttact ggtattaagg ggattttccc cggcctgctt aattaattcc tttcaaatga
      841 aacggagctg ccatgctccg tttacttcgt cattattttt actttgttcc cgcgcagtta
      901 tcaaaagcaa aaggaatagg taaaaatatt cttctcaaat tacagttagt tataaggatt
      961 tccttaactg cttctcctca ccatcatgtt attttcgcca catcataatc ctgggcttgc
     1021 tgaagaataa ttgaaatgat attagtaatt ccactgcctt tggtagagga aagtgctaaa
     1081 taataatcaa ttgttaaatt attgtgcatt tcactactgg aactgtaatc agaaaagata
     1141 gacatgctta gccaatctct atttgattga attgaaagat gtttgttaag gcatggatgc
     1201 aagctataga ttctgatacg gtcaataaaa gagaattgct taacaatttt gcaaaatgta
     1261 ttggcgagta agaaccgcat ttggtacttt ccgggcaacc gccagacgat tctttattgg
     1321 taatgagaat aattaacaat taaagagcat cgcgaaagaa taatgtgtct cgacagggga
     1381 gacacagtac gaatcgacat aaggtgatcg tctgaatcac cagaataaat aaagtcggtg
     1441 atagtaatac gtaacgataa agtaacctga cagcagaaag tctccgagcc tgtgcagggt
     1501 cccaatccgg gattacacat gctggttaat accagtaatt ataatgaggg agtccaaaaa
     1561 acaatgacca acatcaccaa gagaagttta gtagcagctg gcgttctggc tgcgctaatg
     1621 gcagggaatg tcgcgctggc agctgatgta cccgcaggcg tcacactggc ggaaaaacaa
     1681 acactggtac gtaacaatgg ttcagaagtt cagtcattag atccgcacaa aattgaaggt
     1741 gttccggagt ctaatatcag ccgagacctg tttgaaggct tactggtcag cgatcttgac
     1801 ggtcatccag cacctggcgt cgctgaatcc tgggataata aagacgcgaa agtctggacc
     1861 ttccatttgc gtaaagatgc gaaatggtct gatagcacgc cagtcacagc acaagacttt
     1921 gtgtatagct ggcaacgttc tgttgatccg aacactgctt ctccgtatgc cagttatctg
     1981 caatatgggc atatcgccgg tattgatgaa attcttgaag ggaaaaaacc gattaccgat
     2041 ctcggcgtga aagctattga tgatcacaca ttagaagtca ccttaagtga acccgttccg
     2101 tacttctata aattacttgt tcacccatca acttcaccgg tgccaaaagc cgctatcgag
     2161 aaattcggcg aaaaatggac ccagcctggt aatatcgtca ccaacggtgc ctatacctta
     2221 aaagattggg tcgtaaacga acgaatcgtt cttgaacgca gcccgaccta ctggaacaac
     2281 gcgaaaaccg ttattaacca ggtaacctat ttgcctattg cttctgaagt taccgatgtc
     2341 aaccgctacc gtagtggtga aatcgacatg acgtataaca acatgccgat cgaattgttc
     2401 cagaagctga aaaaagagat cccggacgaa gttcacgttg atccatacct gtgcacttac
     2461 tattacgaaa ttaacaacca gaaaccgcca ttcaacgatg tgcgtgtgcg taccgcactg
     2521 aaactaggta tggaccgcga tatcattgtt aataaagtga aagcgcaggg cgacatgccc
     2581 gcctatggtt acactccacc gtatactgat ggcgcaaaat tgactcagcc agaatggttt
     2641 ggctggagcc aggaaaaacg taacgaagaa gcgaaaaaac tgctggctga agcgggttat
     2701 accgcagaca aaccgttgac catcaacctg ctgtataaca cctctgatct gcataaaaag
     2761 ctggcgattg ctgcctcttc attgtggaag aaaaacattg gtgtaaacgt caaactggtt
     2821 aaccaggagt ggaaaacgtt cctcgacacc cgtcaccagg gcacttttga tgtggcccgt
     2881 gcaggctggt gtgcagacta caacgaacca acttccttcc tgaacaccat gctgtcgaac
     2941 agctcgatga ataccgcgca ttataagagc ccggcctttg acagcattat ggcggaaacg
     3001 ctgaaagtga ctgacgaggc gcagcgcaca gctctgtaca ctaaagcaga acaacagctg
     3061 gataaggatt cggccattgt tcctgtttat tactacgtga atgcgcgtct ggtgaaaccg
     3121 tgggttggtg gctataccgg caaagatccg ctggataata cctacacccg gaatatgtac
     3181 attgtgaagc actaatggca atacgtgggg caggagtgtc ctgctccacg gtgtctgatt
     3241 tttatcgcat tacagaaggc acaggccaga aggtagggca atgttaaaat ttattctacg
     3301 tcgctgtctg gaagcgattc cgacgctatt tattcttatt actatttcgt tctttatgat
     3361 gcgcctcgcg ccgggaagcc cttttaccgg cgaacgtact ttaccgccgg aagtgatggc
     3421 caatatcgaa gcgaaatatc atcttaatga tccgatcatg acacagtatt tcagctacct
     3481 gaaacaactg gcgcacggtg atttcggtcc atcgtttaaa tataaagatt attcggtcaa
     3541 tgacctggtt gcatccagtt ttcccgtttc tgccaaactg ggagccgccg catttttcct
     3601 tgcggtaata ctgggtgtta gtgctggcgt tattgtcgca ttaaaacaaa acaccaaatg
     3661 ggactatacc gtgatggggc tggcaatgac cggggttgtt atccccagtt ttgtggttgc
     3721 gccattatta gtcatgatat ttgcgatcat tttgcattgg ctgccgggcg gtggctggaa
     3781 tggtggggcg cttaaattca tgatattgcc gatggtggcg ttgtcactcg cttatatcgc
     3841 cagtattgcg cgtattaccc gaggctctat gattgaagta ttacactcca actttattcg
     3901 tactgcccgg gcgaaagggt tacctatgcg gcggatcatt ttacgccacg cattaaaacc
     3961 tgctctgtta cccgtgctct cttatatggg ccccgcattt gtcggcatta ttaccggttc
     4021 tatggttatc gaaaccattt atggtttgcc ggggattgga caactgttcg ttaatggcgc
     4081 attgaaccgt gactattcct tagtgttaag cctgaccatc ctggttggtg ctttaaccat
     4141 tttgtttaat gccattgtcg atgtgctata tgcggttatc gacccgaaaa tccgttactg
     4201 atactggagc tcgcgatgat gttaagtaag aaaaacagcg agacgctgga aaatttcagt
     4261 gaaaagctgg aggtcgaagg gcgcagcttg tggcaggacg cacgtcgacg ttttatgcat
     4321 aaccgtgcgg cggttgccag tctgatagtg ctggtgctga tcgcgttatt tgtaatcctg
     4381 gcaccgatgc tttcgcagtt tgcctatgac gatactgact gggcgatgat gtccagcgcc
     4441 ccggatatgg agtccggtca ctactttggt actgactcat ccggtcgcga cctgcttgtg
     4501 cgcgttgcga ttggcgggcg tatctcactc atggtcggtg ttgctgcggc actggtggca
     4561 gtggtcgtgg ggacacttta cggttcgctt tccggttatc tgggcggtaa agtggattcg
     4621 gtaatgatgc gtctgctgga aatcctcaac tccttcccat tcatgttctt cgtcattttg
     4681 cttgtgacct ttttcggtca aaacatcctg ctgattttcg tggcgattgg catggtttcc
     4741 tggctggata tggctcgtat tgtgcgtgga caaaccctga gtctgaagcg caaagagttt
     4801 attgaggcgg cacaagttgg cggtgtatcg acatcgggca ttgttattcg ccacattgtg
     4861 ccgaatgtac tcggtgtggt ggtggtctac gcatcacttc tggtgcccag catgatcctc
     4921 tttgaatctt tccttagctt cctggggttg ggtacgcaag agccgttaag cagctggggt
     4981 gcattgctga gtgatggcgc gaactcgatg gaagtctctc catggttatt gttgttccca
     5041 gcgggattcc tcgtggtgac gctgttttgt ttcaacttta tcggcgatgg cttgcgtgat
     5101 gctctcgacc cgaaagatcg ttaaggagtg cagccatgag cgtaattgaa actgcaactg
     5161 tgccgctcgc acaacaacag gctgacgcac tgctgaacgt gaaagatttg cgtgtcacct
     5221 ttagtacccc ggacggcgat gtcacggcgg tcaatgattt gaatttttct ctacgtgccg
     5281 gagaaacgct gggcattgta ggtgagtctg gttcgggtaa atcgcaaact gcatttgcgt
     5341 tgatgggcct gttggctgcc aacggacgta ttggcggatc ggcaaccttc aatgggcgtg
     5401 aaatcctcaa tttgccagag cgtgaactca ataaactgcg cgctgaacaa atctcaatga
     5461 tttttcagga cccaatgact tcgttgaatc cctatatgcg cgtcggtgag cagttgatgg
     5521 aagtgctgat gctgcataag aacatgagca aagctgaggc gtttgaagag tcggtgcgga
     5581 tgctcgatgc ggtaaaaatg ccggaagcac gtaaacgcat gaaaatgtac ccgcacgaat
     5641 tttctggtgg catgcgtcag cgagttatga ttgcgatggc attgctatgt cgacctaagc
     5701 tgctgattgc ggatgaaccc actactgcgc tggacgtcac cgtacaggcg cagatcatga
     5761 cgctattaaa tgaactgaag cgggaattta ataccgccat cattatgatt acccacgatc
     5821 ttggcgtggt ggcggggatc tgcgacaaag tgctggtaat gtacgccggg cgcacgatgg
     5881 aatatggcaa cgcgcgcgat gtcttttatc aacccgttca tccttattct atcggtttgc
     5941 tcaacgcggt gccgcgtctc gatgcggaag gtgaaacaat gttgaccatc cctggtaatc
     6001 cgccaaacct gctgcgatta ccgaaaggtt gcccgttcca gccacgttgt ccgcatgcga
     6061 tggaaatttg tagtagcgca ccgccgctgg aagagtttac gcctggccgt ctgcgtgctt
     6121 gctttaaacc ggtggaggaa ctgttatgaa tgctgtaacc gaaggaagaa aagtcctcct
     6181 cgaaatcgcc gatctgaaag tgcactttga aatcaaagat ggcaaacagt ggttctggca
     6241 accgccgaaa actctcaaag ccgtcgatgg tgtcacgctt cgcctgtatg aaggggaaac
     6301 attaggtgtg gtaggggaat cgggatgcgg taagtccacc tttgctcgcg ccatcattgg
     6361 cctggttaaa gcgaccgacg gtcatgttgc ctggttaggt aaagagttgc tgggcatgaa
     6421 gcccgatgaa tggcgtgccg ttcgcagtga tattcagatg attttccagg atccgttggc
     6481 atcgctaaac ccgcgtatga ccatcggcga gatcatcgct gaaccactgc gtacttatca
     6541 tccgaaaatg tcacgccagg aagttcgcga gcgcgtgaag gcgatgatgc tgaaagtcgg
     6601 gttattgcct aacctgatta accgctatcc gcatgagttc tccggtgggc agtgccagcg
     6661 tatcgggatt gctcgtgctc ttattcttga accgaagctg attatctgcg atgagccgat
     6721 ttcggcgctg gacgtgtcaa ttcaggcgca ggtggtcaac ctgctccagc agctgcaacg
     6781 tgagatggga ttgtcattaa tttttatcgc tcatgacctg gccgtggtaa aacacatttc
     6841 cgatcgtgtg ttggtgatgt atctcggcca tgcggtagaa ctggggacct atgatgaggt
     6901 ctaccacaat ccactacatc cttacaccag ggcattgatg tcggcagtcc ccatacctga
     6961 tccggatctg gagaagaaca aaaccatcca gttactggaa ggggaattac cgtcgccgat
     7021 caacccgcct tccggttgtg ttttccgtac ctgttgcccg attgccggtc cggagtgcgc
     7081 caaaacacgt cctgtgctgg aagggagttt cagacacgcc gtttcttgcc tgaaagtcga
     7141 tccgctttaa acacaataag ggctgacagt tgtcagccct ttttcacgcc gaaagcgatt
     7201 atttattccc gccagatgat atggcaaagt ttgtgatctt tttcgcggca taacagaatg
     7261 cgggcgaaaa catcgttgat ttcaccatcc tcactgtccg ccagaccaat caccacttcg
     7321 gcaaaaaagt ccgggttcaa atcgaaatca acatgttcct gccagtcttc cgccggatcg
     7381 aataactccg cgccgccgcg ctcttcaaac tgaagattga acagcagaac atcagctggg
     7441 tcgagattgt cggcagccag ttcgagaaaa atatcgtagg cctgctcaag cgtttcatct
     7501 tcagtcaggc gattgtttag atccatatcc atagtcacta cctgtttaac ctctgttggc
     7561 gacgttttac agcaacgggc tgaagaagta aaacagtcgc tcggcgacac gttgccataa
     7621 tggacgtttt agccataaac gggcatcgag cagacgtgaa cgcgaaatat aatcgtcctg
     7681 aacggcggca aggtctgcac caaaaccttt atcgtcgatt gccagggtaa tctcgaaatt
     7741 cagccacaga ctacgcatat caaggttaac ggtgccaacc agacttagtt cgccatcgac
     7801 cagcacgctc ttggtatgca gtaacccgcc ttcaaactga taaattttaa ccccagcagc
     7861 aagcagttcc gtaaagaatg cgcgactggc ccagccgacc agcatcgagt cattttttcg
     7921 cggaaggata atactgacat ccaccccgcg ctgcgccgcc gtgcaaatcg catgaagtaa
     7981 atcatcgctt ggcacaaagt agggcgttgt catgatcaaa tattcacgcg ccgaataagc
     8041 cgcagtcaat aatgcctggt gaatgagatc ttccggaaag ccggggccag aagcaattgt
     8101 gtgaatggtg tgaccgctgg cctgttcaaa cggcataata ttgacatctg gtggtggcgg
     8161 cagaatacgt tttccggttt caatctccca gtcgcaggaa taaataatcc ccatcgcggt
     8221 ggcgatgggg ccttccatac gcgccatcag atcaatccat tgccctacgc ccgcatcttg
     8281 tttgaagtag cgaggatcga ccatattcat gctgccggtg tacgcgatgt aattatcgat
     8341 catgatcatc ttgcgatgtt ggcgcaggtc catacggcgt aaaaacacac gcatcagatt
     8401 gacctttaag gcttcgacca cttcaatacc ggcattacgc attagctcgg gccacgggct
     8461 gcggaaaaaa gccacactcc cggcggagtc gagcatcaat cggcaatgaa taccacgccg
     8521 tgcagccgcc attaatgatt cagccacctg gtccgccatg ccgccgggct gccagatata
     8581 aaacaccatc tcaatattat ggcgcgcgag ctggatgtcg cggattaacg cctgcatcac
     8641 atcatctgac tcggtcatca gttgtagctg attccctttg accccagcga tcccctgacg
     8701 acgctcgcaa agcttgaata atggcgcagc gacactgcta ttttcttcgg cgaagatatg
     8761 cttacaggct ttaaggtcgt taagccattt tgcggtggaa ggccacatcg ctctggcgcg
     8821 ctcagcgcgg cgtttgccta aatggagctc gccaacggca agataggcaa taattccgac
     8881 taacggcaga atgtaaataa tcaacagcca ggccatcgcg gagggaactg cgcgtcgttt
     8941 cattagaatg cgtaaagtta cgcctgcaat gagcaaccag tatcccagaa tggccaacca
     9001 actcaccaac gtataaacgg ttgtcataga ttagaaatcc ttttgaaagc gcattgttat
     9061 gagtttacgc atatggattc atctggcaaa taaaaacgcg ggaaaagcac tggtcagcgt
     9121 cgggggtggg ggtataatga ccattctgtt attgcataga gtagttaaca tgaagcggag
     9181 tagaacggaa gtggggcgct ggcgcatgca gcgtcaggct agccgacgta aatcgcgttg
     9241 gcttgagggg caatcgcgcc gaaatatgcg tatccacagc atcaggaagt gcattctaaa
     9301 caaacagcgt aactcgttat tgtttgcgat ctacaatatc taaatatact gggcaccatt
     9361 gcggtgccca ataagtttac tctttcgtct ttaaactcta tttttgcact gattctttac
     9421 tgtcagcttt cgtttcttgt tcatcgttat cgctgaaaat gccatgaccg gagttcaaca
     9481 acattgaaac gagcatatca ttattaatct cttcaccatt taaaactcgc gccagaattt
     9541 cgctgccaaa cagttgcggt gagagaatga tatccggatg taccatctta atcttattca
     9601 ggtttttgct atcactgacg gcgagaactg ttttgacatc actgctcata tcttttgccg
     9661 agagtacaac aaacgcgtta tctgcatcgt tatcactcag cgccagaatg gcccggcaac
     9721 gatcgattcc cgctttcttt aatactgaac tgtcattact gtcaccgggg ataacatcag
     9781 cgttatcgcc taagcgttgc tcaagttgct tgatatcatc ttcaggcaag ttgctgataa
     9841 ccgttacgtt ttgtccgcgt tgattcagtt gcaggatcgt attgatggcg agaatcgaat
     9901 gtccgcaaac aataaaatga tctttacgat gcattgtatg attgtttcct tttacaagtt
     9961 tgttgaatcc cccgcggata agcggaccaa aaattgaggt catagatgtg gcaaaaacgg
    10021 taatgccgga aataatgacc gaaatagtga acaatcgtgc tgattcagaa acagggacaa
    10081 tatcgccata gccgacggtt gacatggttt ctatcgaaaa atagaacgcg gtcatcaaac
    10141 tttctattcg cggattaaaa ccttcgctta aataaagcgc accgtaggtt gagtaaaaca
    10201 gtaatgtcgt gaaactaata aatgcaaaaa ttgtcccggc tgcggcgcta ctgtgggaga
    10261 agtctttgcg cagtatcagc aaaaacacca gcgtaaaaat gcaaaatcca atactaaatt
    10321 tcagccaggg ataaaaatgc agggtgtaaa ttagcgctat caacaacaaa atgatactga
    10381 ttgcccatgc gagctttgcc cggaacagta agccaatgga gttcaggaca agaaagacgc
    10441 cgagcataaa tagtggggcg tttgccagca aggagagatc caactctgaa aatgctttga
    10501 tatggaaaat atcaagcaaa ttgaccgaca taccatagat tgttttaaaa ataagcaatc
    10561 catttaaaaa gacggccagc gcgagaatgt cgtgccgaag ggtcacccat aaatttgttg
    10621 ctgtttgttt gaatgtagcc cagtgactca ctgatacctc tgtaaccaca attttgttta
    10681 attatcttag cgtgcacatt ttctgaacat acatgcagcg cgtgaatgtg ttaaacaata
    10741 gtagatgact ttatccgcag taatcaaagg atttgagcga gcaactgtac ctcaggaaag
    10801 gtcatttttc ctgaatatgc tcacatcata taaagaaata cagataaagt tattatctgc
    10861 ttgtggtagt gaatgcactg accggctata a
//
LOCUS       DAZRUF010000138        10871 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZRUF010000138 DAZRUF010000000
VERSION     DAZRUF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.138"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            54..815
                     /gene="adiY"
                     /locus_tag="ACHXOL_002838"
     CDS             54..815
                     /gene="adiY"
                     /locus_tag="ACHXOL_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR6809771.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            952..2289
                     /gene="adiC"
                     /locus_tag="ACHXOL_002839"
     CDS             952..2289
                     /gene="adiC"
                     /locus_tag="ACHXOL_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR6809772.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2393..4036
                     /gene="eptA"
                     /locus_tag="ACHXOL_002840"
     CDS             2393..4036
                     /gene="eptA"
                     /locus_tag="ACHXOL_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR6809773.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            4033..4701
                     /gene="basR"
                     /locus_tag="ACHXOL_002841"
     CDS             4033..4701
                     /gene="basR"
                     /locus_tag="ACHXOL_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR6809774.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4702..5802
                     /gene="pmrB"
                     /locus_tag="ACHXOL_002842"
     CDS             4702..5802
                     /gene="pmrB"
                     /locus_tag="ACHXOL_002842"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR6809775.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5778..5867)
                     /gene="pmrR"
                     /locus_tag="ACHXOL_002843"
     CDS             complement(5778..5867)
                     /gene="pmrR"
                     /locus_tag="ACHXOL_002843"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR6809776.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5979..7481)
                     /gene="proP"
                     /locus_tag="ACHXOL_002844"
     CDS             complement(5979..7481)
                     /gene="proP"
                     /locus_tag="ACHXOL_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR6809777.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7745..8611)
                     /locus_tag="ACHXOL_002845"
     CDS             complement(7745..8611)
                     /locus_tag="ACHXOL_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR6809778.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(8608..10836)
                     /gene="crfC"
                     /locus_tag="ACHXOL_002846"
     CDS             complement(8608..10836)
                     /gene="crfC"
                     /locus_tag="ACHXOL_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR6809779.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2718 a         2856 c         2674 g         2623 t
ORIGIN      
        1 atcgctttgc tttagcgaga actggtcttt tattcgcact caggagtaca tgtatgagga
       61 tttgcagcga ccaaccttgt attgttttat tgactgaaaa agatgtctgg ataagggtga
      121 atgggaaaga acctattagc cttaaagcta accatatggc gttattaaat tgtgaaaata
      181 atattatcga cgtctcctct cttaacaaca ctttggttgc tcatattagt cacgacatca
      241 tcaaagatta cctccggttt ctgaataaag atctctcgca aataccagta tggcaacgta
      301 gcgctacgcc catactcacc ctgccatgcc tgacgccaga cgtctttcgc gttgccgcgc
      361 aacacagcat gatgcccgca gaaactgagt cagaaaagga acgaacacgc gcattattat
      421 tcacggtgct atcccgtttt ctcgacagta aaaaattcct ttcattaatg atgtatatgt
      481 tacgtaattg tgtaagtgac agcgtttatc aaattattga aagcgatatt cacaaagact
      541 ggaatcttag tatggtagcc agttgtttat gtcttagccc aagtctgtta aagaaaaagc
      601 tgaaaagcga aaacaccagt tatagccaaa taatcaccac ctgccgcatg cgttatgccg
      661 taaatgaatt aatgatggac ggtaaaaata tctctcaggt atcacagtcc tgcggctaca
      721 acagtacgtc gtactttatt tctgtcttta aagacttcta cggtatgacg ccgctgcatt
      781 atgttagtca gcacagagaa cgaactgtcg cctgattttt aaccttaacg aagagctata
      841 ttaataacgg catcagcgat aacccggtcg ataataattc aactatcgaa tgcaggcgta
      901 tgatatgacg taatttattg tcacgaagct cgccttcgca ggagtttaat tatgtcttcg
      961 gatgctgatg ctcacaaagt gggcttaatc cccgtcaccc tgatggtgtc ggggaatatt
     1021 atggggtcag gtgtttttct gttacctgca aacctggcct ccactggcgg gattgccatt
     1081 tatggatggt tggtgacgat tatcggtgcg ctggggctct cgatggtata cgccaaaatg
     1141 tcgttcctcg atccaagtcc tggtggttct tacgcttacg cccgccgctg ctttggcccg
     1201 tttctcggtt atcaaaccaa cgtcctctac tggctggcct gctggatcgg caatatcgct
     1261 atggtggtca ttggcgtagg atatttaagt tacttcttcc cgattctgaa agatccattg
     1321 gtattaacca tcacctgcgt cgtggtgctg tggatcttcg tcctgctgaa cattgtcggt
     1381 ccgaaaatga tcacccgtgt gcaggccgtt gccaccgtgc tggcgctgat ccccatcgtc
     1441 gggattgccg tatttggctg gttctggttc cgtggtgaaa cctatatggc ggcatggaac
     1501 gtcagcggcc tgggcacctt cggtgcgatt caaagtaccc ttaacgttac gctgtggtcg
     1561 ttcatcggtg tggaaagtgc ctccgttgcc gcaggtgtgg tgaaaaaccc gaaacgcaat
     1621 gtccctatcg ccaccattgg tggggtattg attgccgccg tttgctatgt gctctccacc
     1681 accgcgatta tggggatgat ccctaatgcc gcactgcgcg tttctgcctc gccattcggt
     1741 gatgccgcac ggatggcgtt gggtgacacc gctggggcca ttgtttcctt ctgcgcagct
     1801 gcgggttgct taggttcact gggcggctgg acgttgctgg cgggtcaaac ggcgaaagcc
     1861 gctgccgatg acggactgtt cccaccgatt tttgcccgtg taaataaagc gggtacgcca
     1921 gtggcggggc tgattatcgt cggtattttg atgaccatct tccagctcag tagcatttcg
     1981 ccaaacgcaa ccaaagagtt cggtctggta tcttccgtgt cggtcatctt tacactggtg
     2041 ccatatcttt acacctgtgc ggcgttactg ctgctcggcc acggtcactt tggtaaagca
     2101 cgcccggcat atctggctgt tactaccatt gccttcctct actgcatctg ggccgtggtg
     2161 gggtccgggg cgaaagaggt tatgtggtca tttgtcaccc tgatggtcat caccgccatg
     2221 tatgcgctga attacaaccg gctacataaa aacccgtatc ccttagatgc accaataagc
     2281 aaagattaat tccccttaat ccagcaaaca taaaagccaa ccttaagaac ttaaggttgg
     2341 cttaattttg ctttgcgagc atatgcgcac tttgttcgat ggaaacaccg tgatgttgaa
     2401 gcgcctacta aaaagcccct ctttgaattt actcgcctgg ctattgttgg ccgcttttta
     2461 tatctctatc tgcctgaata ttgccttttt taaacaggtg ttgcaggcgc tgccgctgga
     2521 ttcgctgcat aacgtactgg ttttcttgtc gatgccggtc gtcgctttca gcgtgattaa
     2581 tattgtcctg acactaagct ctttcttatg gcttaatcga ccactggcct gcctgtttat
     2641 tctggttggc gcggctgcac aatatttcat aatgacttac ggcatcgtca tcgaccgctc
     2701 gatgattgcc aatattattg ataccactcc agcagaaagt tatgcgctga tgacaccgca
     2761 aatgttatta acgctgggat tcagcggcgt gcttgctgcg ctgattgcct gctggctaaa
     2821 aatcaaacct gccacctcgc gtctgcgcag tgttcttttc cgtggagcca atattctggt
     2881 ttctgtactg ctgattttgc tggtcgccgc actgttttat aaagactacg cctcgttgtt
     2941 ccgcaataac aaagagctgg tgaaatcctt aagcccctct aacagcattg ttgccagctg
     3001 gtcatggtac tcccatcagc gactggcaaa tctaccgctg gtgcgaattg gtgaagacgc
     3061 ccaccgcaac ccgttaatgc agaacgaaaa acgtaaaaat ttgaccatcc tgattgtcgg
     3121 cgaaacctcg cgggcggaga acttctccct caacggctac ccgcgtgaaa ctaacccgcg
     3181 gctggcgaaa gataacgtgg tctatttccc taataccgca tcttgcggca cggcaacggc
     3241 agtttcagta ccgtgcatgt tctcggatat gccgcgtgag cattacaaag aagagctggc
     3301 acagcaccag gaaggcgtgc tggatatcat tcagcaagcg ggcatcaacg tgctgtggaa
     3361 tgacaacgat ggcggctgta aaggtgcctg cgaccgcgtg cctcaccaga acgtcaccgc
     3421 gctgaatctg cctggtcagt gcatcaacgg cgaatgctat gacgaagtgc tgttccacgg
     3481 gctggaagag tacatcaata acctgcaggg tgatggcgtg attgtcttac acaccatcgg
     3541 cagccacggt ccgacctatt acaaccgcta tccgccgcag ttcaggaaat ttaccccaac
     3601 ctgcgacacc aatgagatcc agacctgtac caaagagcaa ctggtgaaca cttacgacaa
     3661 cacgctggtt tacgtcgact atattgttga taaagcgatt aatctgctga aagaacatca
     3721 ggataaattt accaccagcc tggtttatct ttctgaccac ggtgaatcgt taggtgaaaa
     3781 tggcatctat ctgcacggtc tgccttatgc catcgccccg gatagccaaa aacaggtgcc
     3841 gatgctgctg tggctgtcgg aggattatca aaaacggtat caggttgacc agaactgcct
     3901 gcaaaaacag gcgcaaacgc aacactattc acaagacaat ttattctcca cgctattggg
     3961 attaactggc gttgagacga agtattacca ggctgcggat gatgttctgc aaacttgcag
     4021 gagagtgagt gaatgaaaat tctgattgtt gaagacgata cgctgttatt gcagggactg
     4081 attctggcgg cgcaaaccga aggctacacg tgcgatggcg taacaaccgc gcggatggcg
     4141 gaacaaagcc ttgaggccgg tcattacagc ctggtggtac tggatttagg gttacccgac
     4201 gaagatggac tgcattttct cgcccgtatc cggcagaaaa aatacaccct gccggtactg
     4261 atcctcaccg ctcgcgatac gctgaccgac aaaatcgccg ggctggatgt cggtgccgat
     4321 gactatctgg tgaagccttt tgcgctggaa gagttacacg cccgtatccg cgccctgcta
     4381 cgacgccata ataatcaggg cgaaagtgag ctgattgttg gcaatctgac gctgaatatg
     4441 ggtcgccgtc aggtatggat gggcggtgaa gagttgattc tgacgcccaa agaatatgct
     4501 ctgctgtcac ggttaatgct caaagcaggc agtccggtac atcgggaaat tctctacaac
     4561 gacatctata actgggacaa tgaaccctcg accaacaccc tggaagtgca tatccacaat
     4621 ctgcgcgaca aagtgggcaa agcccgtatc cgcaccgtgc gcggctttgg ctatatgctg
     4681 gtcgcgaatg aggaaaacta attgaatctg atgcgttttc tgcgccgacc aatatcgctg
     4741 cgccaacggc tgatattgac catcggggcc attttgttgg tgtttgagct gatcagcgtc
     4801 ttctggctat ggcatgaaag taccgagcag attcagctgt ttgagcaggc gctacgcgac
     4861 aatcgcaaca acgatcgcca catcatgcgt gagatccgcg aggcggtcgc cagcctgatt
     4921 gtccccggcg tctttatggt cagcctgacg ctatttatct gctatcaggc ggtacgccgc
     4981 atcacccgcc cgctggcgga gctgcaaaaa gagctggaag cgcgcaccgc cgacaactta
     5041 acgcccattg ccattcacag cgccaccctc gaaatcgaag cggtggtttc ggcgttgaac
     5101 gatctggtca gtcgcctgac gagcacgctg gataacgaaa ggttatttac cgctgacgtc
     5161 gcgcacgaac tgcgaacgcc actggcgggg gtgcgtttgc atctggaact gctggcgaaa
     5221 acgcatcaca ttgatgtagc accgttagtg gcacggcttg atcagatgat ggagagcgtc
     5281 tcccagctgc tgcaactggc gcgtgccgga cagtcatttt cttccggtaa ttatcaacat
     5341 gtaaaactgc tggaagatgt cattctcccc tcgtatgacg aactcagtac catgctcgac
     5401 cagcgacagc aaaccctgct attgccagag agcgccgccg acatcaccgt tcagggtgat
     5461 gcaaccctgc tgcggatgtt attgcgaaac ctggtagaaa acgcccatcg ttacagccca
     5521 caaggcagca acattatgat taagctgcaa gaagatggcg gagcggtcat ggccgttgaa
     5581 gatgaaggac cgggtattga tgagagtaaa tgcggggagt tgagtaaagc gtttgtacgt
     5641 atggacagcc gttatggcgg gattggtctg gggttaagta ttgtcagccg cattacacag
     5701 ttgcatcacg ggcagttttt cctgcaaaac cggcaagaga cttccggcac gcgggcctgg
     5761 gtacggctga agaaagatca gtacgtagca aaccagatat aaagaaagct gctgaccacc
     5821 agcacactga acacggtagt taaactttca taaacacggt ttttcattgc actctcctcc
     5881 gttaacctgg aggagagtat gcgcgtcaga gattaagtga accttaagag ttcaattggc
     5941 ctgataagac agcgtcacat caggccatcc gtttcagctt attcatcaat tcgcggatgt
     6001 tgctgcacca ggcgggtacg tttcgcctgc aaatcggcaa tctcgtggtc aatatcatcg
     6061 attttctgct cgatattatc gtaatgctcg acgagaattt ccttcgcttc ctgtatatct
     6121 gacgccgccg gtgtcgcacc tttcaacgga cgatttgccg tctctttcat ggttacgccg
     6181 gtgattaaac caatcaccgc cactaccatc aggtaatagg caggcatcat cagattctgc
     6241 gagctttcga ccagccaggc cgccagtgtt ggcgtcagac cggcaaccag caccgaaata
     6301 ttaaatgccg ccgccagcgc gctgtaacgg atatgcgtcg ggaacatcgc tggcaaggta
     6361 gaagccataa cgcccgtaaa gcagttaagg atcaccgcca gcatcagtaa cccggcaaaa
     6421 atcaggccga tgacgttact gttaatcaga ataaacgccg ggatcgccaa cacaaacagg
     6481 gcaacactac caagtagcac aaacggacga cggccaaaac ggtcactcag caagcccatc
     6541 accggctgga caaacagcat accgatcata atggcgataa taatcagcac cccgtggtct
     6601 tcggagtaat gcaggttatg cgacaaataa ctcggcatat aggtcagcaa catgtagtaa
     6661 gtcacgttgg tggcaattac cagaccaata catgtcaaca ggctgcgcca gtatttagtg
     6721 gcaatctctt taaacgagac tttcgggcca tcctgcaaac cttcgcggtc gccctgttcc
     6781 agtttatcaa catgctgctg gaacgccgga gtttcttcca gcgcatggcg caggtaaagc
     6841 ccgataatcc ctaacggcag agcaataaag aacggaatac gccagcccca gtcgaggaag
     6901 ttcgcttcgc cgacaatggt cgaaattaac accaccacgc ccgcacccag cacaaacccg
     6961 gcaatagaac cgaagtccag ccagctgccc ataaagccac gtttacggtc aggggagtat
     7021 tccgcaacaa atatcgacgc cccggtatat tcaccgccga ccgagaaacc ttgtgccatc
     7081 ttacagatca acagcagaat cggtgcccaa ataccaatcg tgtcgtagga cggtattaag
     7141 ccaatacaga acgtactgat cgacataatc acaatagtga tagcgaggat cttctggcga
     7201 ccatatttat cgcccaacat accaaagaag agcccgccaa gcggtcgaat cagaaaggga
     7261 acggagaaag tggcaagtgc agcaaccatc tgcacgctgg ggtcagcccc cgggaaaaaa
     7321 actttaccta atgcgtaagc aacaaaacca taaacaccaa aatcgaacca ttccattgca
     7381 ttacccagtg atgctgcggt aatggcttta cgcagtttac cgtcatcaat aatggtgaca
     7441 tcacgaaggg taatcggttt tacttttttc cttttcagca tagctttcct cgcagagttg
     7501 gcccatttcc gcaaacgcca gaaaaatcag cgcgacggcc gcaaaagggg agcgggaatt
     7561 gcaggatgca atctctgtaa caagcgtagc gggtttgctt acaccctccg gttaaggaat
     7621 gtacaatcaa accagttgac gcctgaattg ggcagttaat gaccttttta ccctaccagt
     7681 gtttaaattt gtgatcaact tcacacatgg atttcgttta ggactcattg atgtaactga
     7741 ttatttacgc caaatttaca gagaagattt cctcgaccat tgcccccacc aaacgctgcg
     7801 ctgaacagag tcgtggcatc cccagcgcga ctgtttgcca gcgttgggtc actgaccagc
     7861 aaacgggctg tttatctgcc gtggaccact ttcccagcca acttaaaaaa tcagcatgct
     7921 cccctcccga agaggcaaac cactgatggt aatagtgacg cgtggtcggt gccgcattga
     7981 tactctctgc cagatgttgt gtcgctttct cgcgcaaatc acccagtaac aacaccaccg
     8041 cttgcgggct tcccagacgg caggcatcgc caaatgcacc ccagcgcaac tcctccagaa
     8101 cgataaaaca gcgcccaggc aacgaccagg cgttatacgc cccagcacgc cagcgggtaa
     8161 aaatttgctc gctctgatca cctgcctgca ccagtaggcc tcgttgcgtt aacgcctttt
     8221 ccttccagcg acaacgggct gaaaaccact catacaaggc ggcaatttgc tgggggagct
     8281 ccgggaaagc agagtgctgg cggtgatcca ttttttgcag cagcgccaga tgctgcgcga
     8341 tctgcgtcag cgccagatga ccgggatcca gcatcgacgc cagtttttcc accattgtca
     8401 gtcgcaggct aagcggcgca tttaacatgc cgccgttcgt ccatgtctga agctgcacac
     8461 gcgaaagaag ttcctgcatc agtcgttcac gaaactgctg ctgatgggct tgtggaaggc
     8521 gggcatcatc gccctgcgcc agatccacta aaaagcgggg ataaaccgac tccagcacgc
     8581 gaccggggcc gtccagtaac gtcttggtca atatcgttct gccgtgaaaa gtgtttgaat
     8641 atcatcgcgt aacagctggg cgtcggtgta aatccagccg tgagtcatca cagtctgctg
     8701 caattgctgc tgcatcagcc tgaccaccga ttcattttgt tgacgcagag ccaggctttc
     8761 gcgtaaacgc gtctgtaatt ccgtcaaaca tgaagcgaac tcagcgcaaa aagtattcat
     8821 gcctgccgta acagattcat cgacctgctc tgccagaact ttagccattt gttggcaata
     8881 aagatcgact tctgcgctta aagctcgttg caacacactg taatcaaccg tctctgtcgg
     8941 ggatttctca tttccccgtc cccagtcggg ctgattcaac cagcgcgaaa aagtctcacg
     9001 cacaacgcct aaacgcgtgc tctgctcgtc cgttgcatcc tggcgcgaaa tgacttcact
     9061 gaacagctgg cgagtgttga cgtggggaac tactccgtga aaaacaggaa aatgaaaccc
     9121 aggacgaaac cctgactcgc tcaattccat tttgacttgt tgctcaatgg gccgaataac
     9181 atcggttaac acccggcaaa gggtggattc aagctcggca aaacgcagcg taaagtcgcg
     9241 actgatggtg ttctgcgccg tctgtaacag tgtctcacag caggtacgaa tctcgcttaa
     9301 cggctccgaa tcatcctgaa acaaggcggc taactgcgca ttcagcgcat cttgctgttg
     9361 acgcagaaag tggttggcgg aggtcagggc cagctcgatt tcatgtttaa tctcgccgct
     9421 cacctgcgcc tgattgagtt gcaatagctg caaactttct tcgacctgat ggatattttg
     9481 ccgcaattgt tcacaagcga cgtttaaccc gtgcgcacga aaatccaggt attcccgcgc
     9541 ctgctgcgcg taattcaaca gtttatgcgc agcagatcgc agagcataca acgaggcgtt
     9601 agcgtaagcg gcatgaagca acgcctgaat tggctgggcg aacagcgaat cttcccacaa
     9661 ctgatcggca gcatgacgaa tatgttcgag gtccgccaga tcggcatgac gccagcgcct
     9721 gccgagcgcg gcatgggcaa aatcttccac ccagcgttgt tgctctggcg ctggtaactt
     9781 accgttgttg gctaactcat ggcgcgctcg atttgccaag tagccccaca tcgacgacac
     9841 cggaaatatc tgctgtggcg taatacagcc tttcatcagc gtcccggaaa tcagtgcccg
     9901 cacctggtcg gcgtcgtcac tgttacgatc ctgttgatcg aacttattga ccagcacata
     9961 cagtggcacc gattgcccca ccgccaaaat cgcctcacgg acctcttcat cggagatcga
    10021 tttcagttgc gtataatcca gcaccgccag taccgccgag gcgcgtgcca gctgctggtt
    10081 aagcattttt tgcagatgcg gttgcccggc ttcatttggc ccgggggtat ccagtaacgt
    10141 caactgaccg ggataactct ccagccccgc cagatggaca aactccactt caatcacggg
    10201 aatatgctca atggcggcgt aagcagaaaa aggaaaatcg acgtccagcg ccttcgccag
    10261 tcgcactaaa tcattcaaac ttttcagaca atgaaaaata ggctgggcac ccagataata
    10321 tttttcgaaa gcgacgccat tttcgatccg ctgcataagc gcacgcatat ctttatctat
    10381 ttccagcaca tcggtcagat gcttaatatc gcaatcacgc aggcgctgtt gtaattgttg
    10441 aattaaacaa tcgattggcg cgacatgtga aaaatgcagt accggttcct tttgcccggg
    10501 cgtatggcga ataagcgtcg gcagcgcagt cattgggcga ttacgattag gcagaacctc
    10561 cgtaccaaca atggcattaa tggtggttga tttccctgct ttcatagtac cgacaatcgc
    10621 cagcaccatt tccagtcggg aaattttact caactcatta ttcagcatcg cgtgacgttc
    10681 ggcgatatta ggctgactcc agggtaaagc cagttgtggc gcgtcgtctc cgggtacaga
    10741 gagaggcatt ttttccagta actgcaactg ttggcgagaa agctgtaaca ggcgttcagc
    10801 ctcctgactt aactcataca gggtctgtgt gtacatagaa aattcttcct taaagcaaat
    10861 tttgttactt t
//
LOCUS       DAZRUF010000139        10859 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZRUF010000139 DAZRUF010000000
VERSION     DAZRUF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.139"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..696)
                     /locus_tag="ACHXOL_002847"
     CDS             complement(<1..696)
                     /locus_tag="ACHXOL_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR6809780.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="ACHXOL_002848"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="ACHXOL_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR6809781.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHXOL_002849"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHXOL_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR6809782.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="ACHXOL_002850"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="ACHXOL_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR6809783.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="ACHXOL_002851"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="ACHXOL_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR6809784.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="ACHXOL_002852"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="ACHXOL_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR6809785.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="ACHXOL_002853"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="ACHXOL_002853"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR6809786.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="ACHXOL_002854"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="ACHXOL_002854"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR6809787.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="ACHXOL_002855"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="ACHXOL_002855"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR6809788.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="ACHXOL_002856"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="ACHXOL_002856"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR6809789.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="ACHXOL_002857"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="ACHXOL_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR6809790.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHXOL_002858"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHXOL_002858"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR6809791.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /gene="acpS"
                     /locus_tag="ACHXOL_002859"
     CDS             10119..10499
                     /gene="acpS"
                     /locus_tag="ACHXOL_002859"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR6809792.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="ACHXOL_002860"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="ACHXOL_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR6809793.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT         2726 a         2673 c         2891 g         2569 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggccc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccacg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagcct ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 tctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctggcag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcaagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataac
//
LOCUS       DAZRUF010000140        10849 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZRUF010000140 DAZRUF010000000
VERSION     DAZRUF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10849
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.140"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            76..417
                     /locus_tag="ACHXOL_002861"
     CDS             76..417
                     /locus_tag="ACHXOL_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR6809794.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(408..1334)
                     /locus_tag="ACHXOL_002862"
     CDS             complement(408..1334)
                     /locus_tag="ACHXOL_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR6809795.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITQVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGWEGAPLYQI"
     gene            1424..2422
                     /locus_tag="ACHXOL_002863"
     CDS             1424..2422
                     /locus_tag="ACHXOL_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR6809796.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2419..2637)
                     /locus_tag="ACHXOL_002864"
     CDS             complement(2419..2637)
                     /locus_tag="ACHXOL_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR6809797.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2639..4654)
                     /gene="ligA"
                     /locus_tag="ACHXOL_002865"
     CDS             complement(2639..4654)
                     /gene="ligA"
                     /locus_tag="ACHXOL_002865"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR6809798.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4725..5711)
                     /gene="zipA"
                     /locus_tag="ACHXOL_002866"
     CDS             complement(4725..5711)
                     /gene="zipA"
                     /locus_tag="ACHXOL_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR6809799.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5941..6702
                     /gene="cysZ"
                     /locus_tag="ACHXOL_002867"
     CDS             5941..6702
                     /gene="cysZ"
                     /locus_tag="ACHXOL_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR6809800.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6887..7858
                     /gene="cysK"
                     /locus_tag="ACHXOL_002868"
     CDS             6887..7858
                     /gene="cysK"
                     /locus_tag="ACHXOL_002868"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR6809801.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8242..8499
                     /gene="ptsH"
                     /locus_tag="ACHXOL_002869"
     CDS             8242..8499
                     /gene="ptsH"
                     /locus_tag="ACHXOL_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR6809802.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8544..10271
                     /gene="ptsI"
                     /locus_tag="ACHXOL_002870"
     CDS             8544..10271
                     /gene="ptsI"
                     /locus_tag="ACHXOL_002870"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR6809803.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10312..10821
                     /gene="crr"
                     /locus_tag="ACHXOL_002871"
     CDS             10312..10821
                     /gene="crr"
                     /locus_tag="ACHXOL_002871"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR6809804.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2640 a         2797 c         2690 g         2722 t
ORIGIN      
        1 tgtaagattg atttttcttc tcaagttcct tgttcttctg ccgatattac ctcgtacagt
       61 gacgaggact gaacaatggc cactatctct tcaacttcaa ttccttccat acaaacacag
      121 agtagtaaca gggcatcaca aggtagcgat gttgcttcaa aaatcgcgcg gataagtcag
      181 caaatcatca aactcactca acaaattaaa gagattgtag acactagcgg cagtgctgaa
      241 gacaaacaaa aacaggccga attgattcaa cagcaaatca ccctactgga aacacaactg
      301 gcgcagttgc agagacagca ggcagaaaag gcgcaggaga aagagcagcg tttgtctctg
      361 aatgtatccc ttctaaatcc tgttgaaaat acgactcata tcgatatcta tatctgatac
      421 aacggagccc cttcccaacc agccattagc gcattgccct ggtctcgtac cacgtcccat
      481 aacgcttcag cggcggtgga aagtgaacgg ttcttcctgc gcaccagcat caactggcgt
      541 tcaacaacct gcgtaatgcg ctttaccacc agtgggctac cttcaggcaa tggtagcgcc
      601 agtgcgggaa gaatactgat gccgatgcct gcggctacca tcgggaacag cgtcgccgga
      661 tgtccaatct cctgcacaat attcgcctga ataccattgc gcgccagcgc tgcgtcaatc
      721 agcggtcggc tgcctgacgc gtaatcctgc aacaccaatt tcgctccctg taatgcctgc
      781 cagggtacat aatcttccac ggccaaagcg ctatcgcgat ggcaaagcag aaagaaaggc
      841 tcggaaagaa tcgcttcaca ttgcagatcg cccactgggc ctggatcgat gacgatgcaa
      901 aaatcgacat ctccctggcg aatactttcc atcacccact gctgcggacg atcgtgcagg
      961 acaaactgaa tatctggata gcggcgatgg ctttcagcaa tgcattgcgg aataagatgc
     1021 gcggaaatgg tctggctggc agcgacccga acttttccgc tcagttgttg ccccatacgc
     1081 ccggtatcgc gcaacgtgct gttaagttca tccagcagtc gctcaagacg caaagccaac
     1141 tgctgccctg catctgtaag caccacttca cgcgtggttc tgtccagcag gcgaacacca
     1201 gtatgatttt ccagctcctt cacactgtga cttactgccg actggctcaa gccgatacgc
     1261 tctcctgcac gactaaaact tttctcctgc gctactgtga cgaaaacctt taattgtttt
     1321 aaagaataat tcatctgttt tcttcatgaa tggatgcaat aaatcaattt tatttctcaa
     1381 actggaagaa gcacaataga accatcgatc atctggagtc tttatgaaac tttttcgtat
     1441 cctcgatcct ttcaccttaa ccctgatcac ggtggtgttg ctggcctctt tctttccggc
     1501 cagaggcgat ttcgtcccct tctttgaaaa tctgaccacc gcagctattg ccctgctgtt
     1561 ctttatgcac ggcgcgaagt tgtcgcgtga ggcgattatt gctggcggtg gtcactggcg
     1621 actgcatttg tgggtaatgt gcagcacctt cgtgctgttt ccgattctgg gtgtactgtt
     1681 tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac tccggttttc tctacttgtg
     1741 cattctcccg gctaccgtgc agtctgcaat cgccttcacg tcaatggcgg gcggtaacgt
     1801 cgcggcggcg gtttgttctg cgtcagcatc cagcctgctg gggattttcc tttcaccatt
     1861 gctagttggt ctggtgatga atgttcacgg tgcagggggc agccttgagc aggtcggtaa
     1921 aattatgctg caactgctgc tgccgtttgt gttggggcat ctttcccggc cgtggattgg
     1981 tgactgggtg tcgcgcaata aaaaatggat tgcgaaaact gaccagacgt ccattctgtt
     2041 ggtggtttat acagcgttca gcgaagccgt cgttaatggt atctggcata aagttggctg
     2101 gggatcattg ctgtttatcg tggtggtcag ctgcgttctt ctggctatcg tgattgtagt
     2161 taacgtcttt atggcacgcc gactgggctt caataaggca gatgaaatta ctatcgtctt
     2221 ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg gcaaacattc tgttccccac
     2281 atcggtgatc ggtatgatgg tgctgcccct gatgattttc catcagatcc aattgatggt
     2341 ctgtgcggtg ctggcgcgtc gatacaaacg ccagaccgaa cagttacagg cgcagcagga
     2401 aagcagcgcc gataaagctt aaagcggacg cttcaggggc tggaccagtt gcgtcagccc
     2461 ctcggtttta atcagcagcg tgatttgcat tagctcaccg agcttccctg ccgggaactc
     2521 atctttgcgg gcaaaccaca gcagatactc ctccggcagg tcgattaagc gacgcccttt
     2581 gtatttgcca aacggcatta tcgtattggc tatttcaatc agctgctctt tttccatctc
     2641 agctacccag caaacgcagc atttccgctt cgtcgatgac ttcaatgccc agttcctgcg
     2701 ccttcgccag tttagatcct gcagcttcac ccgctatcac cagatcggtt ttcttcgaca
     2761 cgctgcccgc gactttcgcg cccagttcga ccagtcgagc tttagcgtca tcacgcgaca
     2821 tctggcttaa gctgcccgta agcaccacgg ttttaccagc aaacgggctg tcaatctctt
     2881 ccgcgttgat aacgatcggc gcaggccagt gaacaccttc cgccaacagc tcgctgatga
     2941 cattgcggtt gctttcttcg gcaaagaagt tgtgaacgtg ggatgcaacg acaatgccaa
     3001 catcaggcac cttttgcagc tcttcaatcg aagcggcttc cagcacttcc agcgtgccga
     3061 aatatgccgc cagacctgct gcggtggcct cgccgacttc acggatgcca agtgcataga
     3121 ggaagcgagc aaaggtggtt tctttcgctt tttccagcgc gttaaccacg ttttgtgccg
     3181 attttggccc catacgctcc agtccggtca gtttgcctgc ggtgagtttg aacagatctg
     3241 ccggagtgtg gacatattct ttttcaacca gctgatcgat gattttgtcg cccattccgt
     3301 caacatccat cgcacggcgg gaaacaaagt gtttcagcga ctctttacgc tgcgcaccgc
     3361 aaatcaggcc accggtacag cgggcaaccg cttcaccttc cacacgctca acgtcagaac
     3421 cacataccgg acaatgcgtc gggaatacaa cctcacgggt atcttccggg cgttcagaaa
     3481 gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg gcgaatcacc actttatcgc
     3541 caatgcgtaa accaagacgt tcgatttcat ccgcattgtg taaggttgcg ttactcacca
     3601 gcacgccagc aacatggaca ggttccagac gcgcaacagg cgtaatcgcg ccagtacgcc
     3661 caacctgaaa ctcgacgtca cgcacaaagg tcatctgctc ctgcgccggg aatttaaacg
     3721 ctaccgccca gcgcggggca cgcgcgacaa agccaagctg ctcctgctgt tccagtgagt
     3781 tgaccttaat caccacgccg tcgatatcaa agcccagcgt cgggcggtct tcttccactt
     3841 tgtgatagaa cgccagcact tcttccgccg attcacaaag cgttacccga tcgctgaccg
     3901 gcaaccccca ctttttaaat tgcagtaaac ggccaagatg agtatccggc agctcgccac
     3961 cttccagaac accaacgcca tagcagaaaa aagtgagcgg tcgcttcgct gtaatacgcg
     4021 gatcaagctg acgcagtgaa ccagctgccg cattacgtgg gttagcaaaa actttcccgc
     4081 ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg aacacttcac
     4141 cacgcacttc cagacgcgcc gggatattct ctccgtgcag cttcagcgga atggcgcgaa
     4201 tagtacgcac attagacgtg atatcttccc cggtggtgcc atcgccacgg gtcgcggcac
     4261 tgactaaaac gccattttca tacagaatac tgacggcaag gccatccagc ttcagctcac
     4321 agcaccaggt gactttctcg ttgtttttca gacggtcctg cacacgtttg ttgaaagcaa
     4381 gaaagctttc ttcatcaaaa acgttatcca gtgacagcat tggtacttca tggcgtatct
     4441 ggctgaaagc cgccagcggc gcagcaccta cacgttgggt aggcgaatca ggcgtaatca
     4501 gttctggatg tttggtttcc agctcgcgca gttcgcgcat cagcctgtcg tattcagcgt
     4561 cgggaatttc cggcgcatcc atcacatgat aaagatattc atgatggcga agcgtcgttc
     4621 gcagttctgt cagttgttgt tcgattgatt ccatatcgca ccatcaatgc taaaaacccc
     4681 cgacaagcgg gggttcgaag aggagttaat ttgccttaag tgtatcaggc gttggcgtct
     4741 ttgacttcgc ggatgatgtc ctggtactcg cgcaatttct gcggagtcat catacggcgc
     4801 tgatcgtcaa gcacgacacc gcccacttca tcggcaatat gctgcgcaga ttgcagcatc
     4861 agcttgaagt tctgcagctc gtcaccgtaa gacggtacct gcataaagat agtgacaccc
     4921 ggagtagtga aatccttcat ttcaggatca aaggttcccg gtttcaccat attcgccagg
     4981 ctgaataacg ccgggccgct gccatccggg ctaagatgac gatggtaaat attcatatcg
     5041 ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt tagctcgcta
     5101 ccgtgatgcg ccgcgacgtt catgataatc accgcttctt tgcgcttcgg tttatccata
     5161 actggagccg gttccgctac aggctcaggc tgtggtgccg ctacgggttc tgcaggctgg
     5221 aaagcctgtt gtgccggttg cggtgctgaa tgcacaggct gcggcgctgg cgcgacctgt
     5281 ggcgaaactg gctgctgcaa cggctgttca ggctgcggct gataggcagg ctgctgcatc
     5341 ggctgcgctg gacgcggagc atgttgcggc ggtacctgcg cttcaggcgg ctgctggatc
     5401 ggttgacgcg gctgcgcaga cgcataaggc ggttggtact ggtgttgcgg cgacggacga
     5461 gcagcctcat gctcctgagc gttagccggg gcatgattca cgcggtgaac acgaacctca
     5521 ccaacgccct catcatcttc gacatcctcg tcataagaat cgtcgtcacg ttttgacttc
     5581 attcgtttta atggccgatc gcggaacata gaagatcgtt ctttacggct ggtccagaaa
     5641 ccatgtacca gtaaagcgat tatggcgatc gcgccaacaa tgattaatat cagacgcaaa
     5701 tcctgcatca ttatattctc tgttgttcta acaccttgcc accacggcaa acatttactc
     5761 actaagagta tttgccgatt acctcaagtg caagtgcact attaactttc acagcacaaa
     5821 gatagatgaa atcgtgcttt ttgctgtttt ttcgaacata tcctaactgt ccattgcgca
     5881 attacccggt aaaatacgca gaattttcct gggattggtc aaaaggagct catcctgatt
     5941 atggtttcat cattcacatc tgccccacgc agcggttttt actattttgc gcaaggctgg
     6001 aagctcgtct cgcaacctgg gattcggcgt ttcgttattt taccgctgct ggtcaatatt
     6061 ttgttgatgg ggggcgcatt ctggtggctc tttacacagc tcgatgtctg gatcccgact
     6121 ctcatgagtt acgttccgga ctggctacaa tggctgagtt atctgttgtg gcctctggct
     6181 gtcatctctg tgctgttagt gtttggctat ttcttctcca cgattgctaa ctggattgcc
     6241 gctccgttta acggtttatt ggctgaacaa ctggaagcac gattgactgg cgctacaccg
     6301 ccagataccg ggattttcgg tatcatgaaa gatgtgccgc gaatcatgaa acgcgaatgg
     6361 caaaaatttg cctggtatct gccgcgcgca attgtattac taattcttta cttcatccct
     6421 ggtattgggc aaaccgtcgc gccggtactg tggttcctgt ttagcgcctg gatgttagcc
     6481 atccagtatt gcgattaccc cttcgataac cacaaagtgc cgtttaaaga gatgcgcacc
     6541 gccctgcgca cacgcaaaat caccaatatg cagtttggtg ctttaaccag cctgtttacg
     6601 atgatcccgc tgcttaatct gttcatcatg cccgttgccg tttgtggcgc gacggcgatg
     6661 tgggtcgatt gctatcgcga taaacacgcg atgtggcggt aacaatctac cggttatttt
     6721 gtaaaccgtt tgtgtgaaac aggggtggct tatgccgccc cttattccat cttgcatgtc
     6781 attatttccc ttctgtatat agatatgcta aatccttact tccgcatatt ctctgagcgg
     6841 gtatgctacc tgttgtatcc caatttcata cagttaagga caggccatga gtaagatttt
     6901 tgaagataac tcgctgacta tcggtcacac gccgctggtt cgcctgaatc gcatcggtaa
     6961 cggacgcatt ctggcgaagg tggaatctcg taaccccagc ttcagcgtta agtgccgtat
     7021 cggtgccaac atgatttggg atgccgaaaa gcgcggcgtg ctgaaaccag gcgttgaact
     7081 ggttgaaccg accagcggta ataccgggat tgcactggcc tatgtagctg ccgctcgcgg
     7141 ttacaaactc accctgacca tgccagaaac catgagtatt gaacgccgca agctgctgaa
     7201 agcgttaggt gcaaacctgg tgctgacgga aggtgctaaa ggcatgaaag gcgcaatcca
     7261 aaaagcagaa gaaattgtcg ccagcaatcc agagaaatac ctgctgctgc aacaattcag
     7321 caatccggca aaccctgaaa ttcacgaaaa aaccaccggt ccggagatct gggaagatac
     7381 cgacggtcag gttgatgtat ttattgctgg cgttgggact ggcggtacgc tgactggcgt
     7441 cagccgctac attaaaggca ccaaaggcaa gaccgatctt atctctgtcg ccgttgagcc
     7501 aaccgattct ccagttatcg cccaggcgct ggcaggtgaa gagattaaac ctggcccgca
     7561 taaaattcag ggtattggcg ctggttttat cccggctaac ctcgatctca agctggtcga
     7621 taaagtcatt ggcatcacca atgaagaagc catttctacc gcccgtcgtc taatggaaga
     7681 agaaggtatt cttgcaggta tctcttctgg agcagctgtt gccgcggcgt tgaaactaca
     7741 agaagatgaa agctttacca ataagaatat tgtggttatt ctaccatcat cgggtgagcg
     7801 ttatttaagc accgcattgt ttgccgatct cttcactgag aaagaattgc aacagtaatg
     7861 ccagcttgtt aaaaatgcgt aaaaaagcac ctttttaggt gcttttttgt ggcctgcttc
     7921 aaactttcgc ccctcctggc attgattcag cctgtcggaa ctggtattta accagactaa
     7981 ttattttgat gcgcgaaatt aatcgttaca ggaaaagcca aagctgaatc gattttatga
     8041 tttggttcaa ttcttccttt aacggcataa tgtttaatga cgtacgaaac gtcagcggtc
     8101 aacacccgcc agcaatggac tgtattgcgc tcttcgtgcg tcgcgtctgt taaaaactgg
     8161 cgctaacaat acaggctaaa gtcgaaccgc caggctagac tttagttcca caacactaaa
     8221 cctataagtt ggggaaatac aatgttccag caagaagtta ccattaccgc tccgaacggt
     8281 ctgcacaccc gccctgctgc ccagtttgta aaagaagcta agggcttcac ttctgaaatt
     8341 actgtgactt ccaacggcaa aagcgccagc gcgaaaagcc tgtttaaact gcagactctg
     8401 ggcctgactc aaggtaccgt tgtgactatc tccgcagaag gcgaagacga gcagaaagcg
     8461 gttgaacatc tggttaaact gatggcggaa ctcgagtaat ttcccgggtt cttttaaaaa
     8521 tcagtcacaa gtaaggtagg gttatgattt caggcatttt agcatccccg ggtatcgctt
     8581 tcggtaaagc tctgcttctg aaagaagacg aaattgtcat tgaccggaaa aaaatttctg
     8641 ccgaccaggt tgatcaggaa gttgaacgtt ttctgagcgg tcgtgccaag gcatcagccc
     8701 agctggaaac gatcaaaacg aaagctggtg aaacgttcgg tgaagaaaaa gaagccatct
     8761 ttgaagggca tattatgctg ctcgaagatg aggagctgga gcaggaaatc atagccctga
     8821 ttaaagataa acacatgaca gctgacgcag ctgctcatga agttatcgaa ggtcaggctt
     8881 ctgccctgga agagctggat gatgaatacc tgaaagaacg tgcggctgac gtacgtgata
     8941 tcggtaagcg cctgctgcgc aacatcctgg gcctgaagat tatcgacctg agcgccattc
     9001 aggatgaagt cattctggtt gccgctgacc tgacgccgtc cgaaaccgca cagctgaacc
     9061 tgaagaaggt gctgggtttc atcaccgacg cgggtggccg tacttcccac acctctatca
     9121 tggcgcgttc tctggaactg cctgctatcg tgggtaccgg tagcgtcacc tctcaggtga
     9181 aaaatgacga ctatctgatt ctggatgccg taaataatca ggtttacgtc aatccaacca
     9241 acgaagttat tgataaaatg cgcgctgttc aggagcaagt ggcttctgaa aaagcagagc
     9301 ttgctaaact gaaagatctg ccagctatta cgctggacgg tcaccaggta gaagtatgcg
     9361 ctaacattgg tacggttcgt gacgttgaag gtgcagagcg taacggcgct gaaggcgttg
     9421 gtctgtatcg tactgagttc ctgttcatgg accgcgacgc gctgcccact gaagaagaac
     9481 agtttgctgc ttacaaagca gtggctgaag cgtgtggctc gcaggcggtt atcgttcgta
     9541 ccatggacat cggcggcgac aaagagctgc catacatgaa cttcccgaaa gaagagaacc
     9601 cgttcctcgg ctggcgcgct atccgtatcg cgatggatcg taaagagatc ctgcgcgatc
     9661 agctccgcgc tatcctgcgt gcctcggctt tcggtaaatt gcgcattatg ttcccgatga
     9721 tcatctctgt tgaagaagtg cgtgcactgc gcaaagagat cgaaatctac aaacaggaac
     9781 tgcgcgacga aggtaaagcg tttgacgagt caattgaaat cggcgtaatg gtggaaacac
     9841 cggctgccgc aacaattgca cgtcatttag ccaaagaagt tgatttcttt agtatcggca
     9901 ccaatgattt aacgcagtac actctggcag ttgaccgtgg taatgatatg atttcacacc
     9961 tttaccagcc aatgtcaccg tccgtgctga acttgatcaa gcaagttatt gatgcttctc
    10021 atgctgaagg caaatggact ggcatgtgtg gtgagcttgc tggcgatgaa cgtgctacac
    10081 ttctgttgct ggggatgggt ctggacgaat tctctatgag cgccatttct atcccgcgca
    10141 ttaagaagat tatccgtaac acgaacttcg aagatgcgaa ggtgttagca gagcaggctc
    10201 ttgctcaacc gacaacggac gagttaatga cgctggttaa caagttcatt gaagaaaaaa
    10261 caatctgcta atccacgaga tgcggcccaa tttactgctt aggagaagat catgggtttg
    10321 ttcgataaac tgaaatctct ggtttccgac gacaagaagg ataccggaac tattgagatc
    10381 attgctccgc tctctggcga gatcgtcaat atcgaagacg tgccggatgt cgtttttgcg
    10441 gaaaaaatcg ttggtgatgg tattgctatc aaaccaacgg gtaacaaaat ggtcgcgcct
    10501 gtagacggta ccattggtaa aatctttgaa accaatcacg cgttctctat cgaatctgat
    10561 agcggcgttg aactgttcgt ccacttcggt atcgacaccg ttgaactgaa aggcgaaggc
    10621 ttcaagcgta ttgctgaaga aggtcagcgc gtgaaagttg gcgatactgt cattgaattt
    10681 gatctgccgc tgctggaaga gaaagccaag tctaccctga ctccggttgt tatctccaac
    10741 atggacgaaa tcaaagaact gatcaaactg tccggtagcg taaccgtggg tgaaaccccg
    10801 gttatccgca tcaagaagta attcttgccg cagtgaaaaa tggcgccca
//
LOCUS       DAZRUF010000141        10834 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZRUF010000141 DAZRUF010000000
VERSION     DAZRUF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.141"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..174
                     /locus_tag="ACHXOL_002872"
     CDS             <1..174
                     /locus_tag="ACHXOL_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809805.1"
                     /translation="EFARMIFAYINDKSIEILLLSCFAASDQKAIQRYIPQSQDGEDF
                     RSHLQYKLPLSAH"
     gene            385..708
                     /locus_tag="ACHXOL_002873"
     CDS             385..708
                     /locus_tag="ACHXOL_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR6809806.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            705..1535
                     /gene="amiD"
                     /locus_tag="ACHXOL_002874"
     CDS             705..1535
                     /gene="amiD"
                     /locus_tag="ACHXOL_002874"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR6809807.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1532..2545)
                     /locus_tag="ACHXOL_002875"
     CDS             complement(1532..2545)
                     /locus_tag="ACHXOL_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR6809808.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2644..4074)
                     /locus_tag="ACHXOL_002876"
     CDS             complement(2644..4074)
                     /locus_tag="ACHXOL_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR6809809.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4085..5086)
                     /gene="ltaE"
                     /locus_tag="ACHXOL_002877"
     CDS             complement(4085..5086)
                     /gene="ltaE"
                     /locus_tag="ACHXOL_002877"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR6809810.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5123..6841)
                     /gene="poxB"
                     /locus_tag="ACHXOL_002878"
     CDS             complement(5123..6841)
                     /gene="poxB"
                     /locus_tag="ACHXOL_002878"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR6809811.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(6974..7942)
                     /gene="hcr"
                     /locus_tag="ACHXOL_002879"
     CDS             complement(6974..7942)
                     /gene="hcr"
                     /locus_tag="ACHXOL_002879"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR6809812.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(7954..9606)
                     /gene="hcp"
                     /locus_tag="ACHXOL_002880"
                     /gene_synonym="priS"
     CDS             complement(7954..9606)
                     /gene="hcp"
                     /locus_tag="ACHXOL_002880"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR6809813.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9750..10649)
                     /gene="lysO"
                     /locus_tag="ACHXOL_002881"
     CDS             complement(9750..10649)
                     /gene="lysO"
                     /locus_tag="ACHXOL_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR6809814.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         2626 a         2875 c         2869 g         2464 t
ORIGIN      
        1 gaatttgcaa gaatgatctt cgcttatatt aatgacaaaa gcattgagat cttattactt
       61 tcttgttttg cggcgtctga ccaaaaagca atccagaggt atatacctca gtcacaagat
      121 ggggaagact ttaggagtca tttacaatat aagttgcctc tttccgctca ttaatgttgt
      181 tggtgcaaca taataagaca ggtaagcggt accctataat gaaaccaata tcgggttatg
      241 caacggataa tcgtaaagac ttaagtgggt gtttttgaca caatgagatg atttcctaaa
      301 ctgatcaaaa ccttcttccc aacatggcat atcagttcga taatgcttta gagatcgaag
      361 gcttaacaaa caaggagatc gatcatgcaa ttttctacaa ccccaactct ggaaggccag
      421 accatcgttg aatattgcgg tgtggtgacc ggcgaagcga ttttaggtgc caatattttc
      481 cgtgatttct ttgccggtat ccgcgatatc gttggcggac gttccggtgc gtatgaaaaa
      541 gaactgcgta aagcacggga gatcgccttt gaggaattag gctcccaggc gcgggcgctg
      601 ggggccgatg ccgtcgtcgg tattgatatc gactacgaaa cggtcgggca aaacggcagt
      661 atgctgatgg ttagcgtcag cggtacggcg gtgaaaacgc gtcgatgaga agagtcttct
      721 ggctggtcgc tgccgctctg ttattggcag ggtgtgcagg cgaaaaaggc attgtcgaga
      781 aagagggata tcagcttgat acccgacgcc aggcgcaggc ggcgtatccg cgcattaaag
      841 tgctggtgat ccactacacc gcagatgatt ttgatagctc gctggcgaca ctgaccgata
      901 agcaggtcag ctcgcattat ctggtccctg cggtaccacc gcgatacaac ggtaaaccgc
      961 gcatctggca actggtgccg gaacaagaac tggcctggca tgcggggatt agcgcctggc
     1021 gcggggcaac gcgccttaac gacacctcta ttggcattga gctggaaaac cgtggctggc
     1081 aaaaatcggc cggagtgaaa tattttgccc cgtttgaacc gacacagatt caggcgctta
     1141 ttccactggc gaaagatatt attgcccgtt atcacatcaa gccggaaaac gtagtggcac
     1201 atgcggatat cgcaccgcag cgcaaagacg atccggggcc attatttccc tggcagcaac
     1261 tggcgcagca ggggattggt gcctggccgg atgcgcagcg ggttaacttt taccttgccg
     1321 ggcgcgcgcc gcacacaccg gtagataccg cgtcattgct ggagcttttg gcgcgctacg
     1381 gttatgacgt taaacctgat atgacaccgc gcgagcagcg gcgcgtgatt atggcattcc
     1441 agatgcattt ccgcccgacg ttatataacg gcgaagcgga tgcagaaact caggcgattg
     1501 ccgaagcatt gctggagaaa tacgggcagg attagcgcgg cagttttccg tggtcgcgta
     1561 gccaggcggc agttttctcg ataccttcat ctagggtgat gatcggctga taacctaact
     1621 cttcctgcgc acgcgtaata tccagcgtaa agtcaaaatt caacttggag acgccgtagt
     1681 gggtcagcgg cggctctttt gctgacttgc ggcctaaacg ctccatgctg cgggcgatca
     1741 tatccagcat cgggtagggg acggaacgaa tacgacagtc aatattcaac tcgtcgatca
     1801 gcttctgcac gatgctgcgc agtgtgcgat gctcgccgtt ggtgatattg tacacacgcc
     1861 cggaaggtag cttatcgcag gcttcctggc ttgccagcca cattgcgtgc acggtatttt
     1921 catagtaggt catatcgacc agcgcactgc cgccatgcgg taacagaata ctgccgtagt
     1981 ggtgcatcat atgcgccaga cggggaataa agactttatc gtgcggtccg aacagacttt
     2041 gtgggcgcag aatagtaaag cgcgtttgtg gattcgcctg cgaaagcata ttgatcactt
     2101 cttcgctggc tgctttgctg cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt
     2161 ctttaatatc gcgatggtgg tgataatcga agtacaggga gggggaagag atatgaataa
     2221 agttacgcac accccaggcg acagcccatt cacccaggcg gcgagtggcg cgaacgttag
     2281 ccagatcgaa agcctgttgt gtcccccagg gtgaggtaaa gctggagcag tgccacagcg
     2341 tatcaatgcc cgcgagcatc actttagctt gtgatgaaac cagctcggtc agatccgccg
     2401 gaacaaactc tgcgcccatt ttttccagca atttgcccat tgcctcgttg cgaccggtcg
     2461 ctcgcacgct gatgcctttc tggcataaaa actctaccgc gtttcgacct aagccgctgg
     2521 tggcgccggt aaccagtacc ttcatatcaa tccactgttg ttgagaaaat gacgtgcgca
     2581 ttcttccgtg atttccccca tgatgcaatg ggaaacatga aagaataacg caggttttgt
     2641 cgattaatct gtgctttgtt ctgccagtct ggcgatttgt tttgccattc cgcgaaaaat
     2701 aaacagatgc gcggggatca tcaataacca gtaaaacagc cccggcatac cgtgcggatg
     2761 ccagaaagcg cggacatcga tagtacgata gtcgccttta tcttccaggc taaaacacag
     2821 tcgtcccagc cccgacgctt tcatgccaaa taacaacgta agttgttttt ccggttcaac
     2881 gacaatcact ttccagctat ccaccgcatc gccagtctgt aaatattcgc gctccgggcg
     2941 gcctttcgcc agcttatgac cgatcgcgcg gtccatcaac gcccgtgtct gccacaaaat
     3001 attgccaaag aaataacgct ctttaccgcc gatttggttc actacctgcc ataaagcagc
     3061 aaggctggcg gacgttttaa cggtaaaccc cgcctgtttg gcaaaataac cgtactccgg
     3121 tcgccagcgg gcaaaggcct gagcgtcgta gccccagtcg ctggagttga ccagtttttc
     3181 ctcctctttc aacgtgctac gtaccgcgtc atcgaaagcg atcagccgtt gtgggatgag
     3241 tgcacgtagc gcggtatcat ccgccagcag atcgtgtttc agcccctgaa tcaacgccct
     3301 ggcggtggtg ggcggtacgg aagtaatcac attgagaaac cacaccgaaa tccagcgggt
     3361 ggggaggggg atggggatca accagcggcg cttaccgctc accgccataa aatgttcaaa
     3421 ctgttgctga taactgagca cctctggtcc ggcggcttcg aagatgcggt gttcgctggc
     3481 cggatgatct aacaacgcca ccagatagtg cagcaagttt tccagcgcga tgggcgtggt
     3541 gcgtgaacgt acccagcgtg gcggcgttaa cactggcagg ttgtagacca tatcgcgcat
     3601 gacttcgaac gccgctgaac ctgcgccaac gataattccg gcccgaagtt cggtcacagg
     3661 tacattcgct tcacgaagaa tgtccgccgt agcctgacga gcacgcagat gatccgactg
     3721 ctcatgtggc ggggcctgca acgaactgag aaagattaat tgcttaactg gtacttcacg
     3781 tagcgcatcg cggacgttga gagccacctg gcgctcctga gcgataaaat cgccgccttc
     3841 gcccatgctg tgcaccagaa aatagacggt atcgatatcc tgcaacaggg ccggaaggtt
     3901 atccggccag ctgagatcga ctttatggca actgacgttt gccagttgca gctttgcaag
     3961 cctgtcgaca tgacgtgccg ccgccaggat ctgatgccct tgctggctga gtgtgcgcac
     4021 cagatgctga ccaatgtagc cactggcacc gagaactaaa atgcgttgcg gcacgtctct
     4081 ctccttaacg cgccaggaat gcgcgccagt gggcggcgac ttccgccagt tgttcgcgcg
     4141 agacgtcaag atgcgtcacc aggcggacaa tcggcgaggc gttaatcagc acgtttctcg
     4201 ctttcatgta ttcgcctaac gcggcagcat tttcttcccc gacgcgaaca aacagcatat
     4261 tagtgtcctg acgcatcaca tccgcgcctg cttcacgcag ttgctccgcc atccaggcgg
     4321 cgttgtcgtg atcttcctgc aaccgtgcga cgttattttt cagggcatac atcccggcgg
     4381 cagccagaat cccggactga cgcatcccgc caccggccat tttccgccag cggatggcac
     4441 gtttaatgta atcacgatta ccgacgagta atgaaccgac tggcgtccca agacctttcg
     4501 acaggcaaat ggtgaacgaa tcacaatatt gcgtgatctc tttcagttcg cagccgtaag
     4561 ccaccacggc attaaagatg cgcgcaccgt caacatgcag cgccagattg cgcttgcggg
     4621 taaattccca tgcttctttc aggtattcac gcggcagcac tttgccgttg tgggtgtttt
     4681 ctagactgag taatttggtg cgggcgaaat ggatatcgtc gggtttgatt ttcatcgcca
     4741 ctttatccag cggtagcgtg ccgtcggcag ccgcgtcgat gggttgcggt tgaatactgc
     4801 ccagcaccgc tgcgccaccg gcttcaaaca gataattatg cgcggcctga ccgacaatat
     4861 actcttcgcc gcgttcgcag tgactgagca gagcgaccag gttagcctgg gtgccggtcg
     4921 gcagaaaaat ggcggcttct ttaccggaaa gctctgcggc gtagtcctgc agagcattaa
     4981 cggtagggtc gtctccgtaa acgtcgtccc caaccggggc ggccatcatt gcttcgagca
     5041 tggcgcggct cggtcgggta acggtatcac tgcgtaaatc aatcatggca tgtccttatt
     5101 atgacgggaa atgccaccct ttttacctta gccagttcgt tttcgccagt tcgatcactt
     5161 catcgccgcg tccgctgatg attgcgcgca acatatacaa gctaaatcct ttggcctgtt
     5221 cgagtttgat ctgcggtgga atggctaact cttctttggc gaccaccaca tccaccagca
     5281 ccggaccgtc gatggagaag gcgcgttgca gggcttcatc aacttcagac gctttttcta
     5341 cacggatacc cgtaatgccg cacgcttcgg caatgcgggc aaagtttgtg tcgtgtagtt
     5401 cggtgccgtc agtcaaatag ccaccagctt tcatctccat cgccacaaag cccagcacgc
     5461 tgttgttaaa gacgacaatt ttcactggca gtttcatctg cactactgag aggaaatcgc
     5521 ccatcaacat gctaaaaccg ccatcgccgc acatggcgac cacctgacgt tctggctctg
     5581 tcgcctgcgc acccagcgcc tgcggcatgg cgttagccat cgaaccgtgg ttaaacgaac
     5641 ctaacaggcg acgcttgccg ttcattttta gataacgtgc cgcccacacc gttggcgtac
     5701 caacgtcaca ggtgaaaata gcgtcatcgg cggcaaaatg actaatttgc tgcgccagat
     5761 attgcgggtg aatggctttc tcgctcggtt tagctaaatc gtccagccct ttgcgggcgt
     5821 cgcggtaatc ttccagcgct ttatccagaa acttgcgatc ggctttttct tccaccaatg
     5881 gaagcaatgc acgcagagtc gacttgatat cgccgaccag tgccatatcc accttgctgt
     5941 gagcgccgat gctggctggg ttgatatcaa tctgaatgat tttggcatcg gtcgggtaga
     6001 aggcgcggta gggaaattgc gtgccgagta gcactaacgt gtcggcgttc atcatggtat
     6061 ggaaacctga cgagaagccg attaacccgg tcattccaac atcatacgga ttatcgtatt
     6121 cgacatgttc tttaccgcgc agggcatgaa caataggcgc tttaattttc ccggcaaact
     6181 caactaactc tttatgcgcc cccgcgcagc cgctgccaca catcagggcg atattgctgg
     6241 aataacgcag cagttgcgcc agtttgcgta actcttcttc ttccggcgtc acgactggtt
     6301 gtggcgcatg ataccagtgc gtggttgccc cttctggcgc aggttttaac gccacgtcgc
     6361 ctggtaacac gacaaccgaa acgccacggt taagcaccgc tttgcgcatg gcaatcgcca
     6421 gtacttgtgg gatctgctcc gggctggaaa ccagctcgca atagtgacta cattcgcgga
     6481 atagctcttg tgggtgggtt tcctggaaat agccgctgcc aatttcgctg gagggaatat
     6541 gagcggcaat cgccagtacc ggaacgtgat tgcggtggca atcgaacagg ccgttgatta
     6601 agtgcaggtt gccggggccg cacgatccgg cgcagaccgc cagttctccg ctaagttgtg
     6661 cttcagcgcc agcggcaaag gccgccactt cttcgtggcg ggtggacatc cactcgatgg
     6721 tgcccatgcg attaagactg tcactaagac cgttcagaga gtcgcctgtg actccccaga
     6781 tgcgtttcac ccctgccgat tcgagtgttt tggcgatata agctgcaacc gtttgtttca
     6841 tggttctcca tctcctgaat gtgataacgg taacaagttt agttcatctg acggaggggg
     6901 aagggatggg agagaaagga ggcactaacg gttaaatagc ccgatgaaag gaatatcatc
     6961 gggcataagg cgattatgcg agaaccaaat ccccctgcgg atggcaggag caggccagta
     7021 cgtaaccttc agcgatttcg gcgtcggtca gcgtcattgt actgctcacc gtatattcac
     7081 cggaaaccac ttttgtctta cagcagccgc aaacacccgc acggcaggca gcgacaaccg
     7141 gaacgttatt gctttccagc gcctccagta gcgtggtgcc aaccggggcg taaaattctc
     7201 gtgccggttg cagtttggtg aatttcagac cgctggtcgc cgcttctgct actggggtga
     7261 agaatttctc tttaaagaaa cgcgtcacgc cgagcgcttt cacttcctgc tctacccaat
     7321 ccatatacgg agccgggccg caggtcatca cggtacgtga agctaagtca ggtacacctg
     7381 ccagcagttc gcgagtgaga cgaccagcga taaagccttc ggtaacgtta ttttctgcca
     7441 ccagcgttac cggatagtta cgccactcat cggcgaaaat aacatcctgc ggcgtacgca
     7501 cgttgtagat cacccgcaca tcggcctgtg gacggttctt cgcaagccag cgacgcatcg
     7561 acataatcgg cgtgacgccg cagcctgccg ccagcaacag gaatttatct tctgctttat
     7621 cgtcgcaggt aaattccccc atcgcgtccg aaagccagag ataatcaccg cgttttacat
     7681 cgcgcgtcag ccactgggag ccgacaccgt catcaatccg ccgcacggtc agggtgatat
     7741 attcactcac gcctggcgtg gaggaaatgg tgtaagcacg cagcgtttcc gctgagtttc
     7801 gcacgctgac cagtgcatat tgcccggcgc gatatgggta gtaatcgtgg caaatcaggg
     7861 aaatcgtcca cacatccggc gtttcttgcg taatgtgatg aacctgcatc cgccacgggc
     7921 attgattcgt tggcatcgtc atcgacaaac tccttacgcg ctcaacagtt gcttcatgtc
     7981 ttcttcaaca gtggtgatag aacgcaggcc gaatttctcg ttcagcaccg ccagcaggtc
     8041 tggtgtcagg aaaccaggtg cagtcgggcc ggtgacgata tttttcacgc ccagagaaag
     8101 cagcgtcagc agaatgacga tcgctttctg ttcaaaccag gagagcacca gcgacagcgg
     8161 cagatcgttc acaccacagc ccagtttctc tgccagagtg acagccagaa taatcgctga
     8221 gtaagcatcg ttacattgac ctgcatctac cagacgcggc agaccttcga tatcgccaaa
     8281 ctcaagtttg ttaaagcgat atttaccaca ggcgagggtc aggatcaggc agtcatccgg
     8341 cacgctggtg gcgaaatcgg tgaagtagtg gcgctcgccg cgtgcgccgt cacagccacc
     8401 aagcaggaag atatgacgca gtttttcacg gctcaccaga tcaatcagcg tatcagcagc
     8461 gccaagcagc gtctggcgac caaaacccac ggtgataagg tgcggaattt cgctgtacgg
     8521 gaagcctgcc atctgttgcg cctgggtgat aaccgcagag aaatcatcac cgtccagatg
     8581 acgcacgcca ggccagccaa caatgctgcg ggtccagata cgatcgtcat aagcgcctac
     8641 ggttgggtcg atgatgcagt tcgaggtcat cacgatgggg ccagggaaac gagcgaactc
     8701 cacttgctga ttctgccagc cgctgccgta gttaccgacc agatgcttga atttacgcag
     8761 ctccgggtag ccatgcgcag gcagcatttc gccgtgggtg tagacattaa cgcccgtgcc
     8821 ttcggtctgt tccagcaggt tgtagagatc tttgagatcg tgaccggaaa tcagaatgca
     8881 tttacccgcc gtcgctttga cgttgacctg ggttggcgtc gggtgaccgt atttaccggt
     8941 ttcgcctgca tccagaatgc tcatcacttt gaagttcatc tggccgattt ccattgaaca
     9001 ctcaagaagc gcgttcatat cggcaggcca ggtccccagc cacgccatga ttttatggta
     9061 ctgggcataa atatcgttgt cgtattgacc gagaacatgc gcgtgttcca tataggccgc
     9121 cgcacctttc aggccataca ggcacagcag acgcaggccg agaatgtttt cgccaatcgc
     9181 cgctttatct ttgttagggg taaattctgc tgcctgacgt tgcagctcgc cgagatcatc
     9241 gctcaccagt tgcaggtcag ccatcgggtt atcgacgcgc gcgttggcat ctacagccag
     9301 gcattgtgct ttcagcgcct cgcgcaaggc aatcgcttca cgagcgtagc cgacaatacg
     9361 cggagaatcg aagttaacgt tggtcagggt tgagaaaaag gcacgtggcg cgaagctgtc
     9421 aacatcgtgg ttgatgatgc cgtattcaca cgcttttacc gcccaggcag aaagcccttg
     9481 cagcgccgcg atgagtaaat cctgaaggtc agaagtttcc gccgttttac cacacatccc
     9541 ctgcgcgtat gagcagccgt ttcctgccgg agtacggata gtttgttcac attgcacaca
     9601 aaacatgatc acacctttta aagttatatt taatatacat gtttaaggtt aagacgctta
     9661 acgcggggat aaaagggatt tttcatgcaa ctttaaggga gattgattta gcgcaatttt
     9721 ggcggcaggg atctaccgcc agagaggtat tacgcagaga aaaaggcgat gaggatcggc
     9781 actaacaggc taagaataaa accgtgaaca attgccgccg ggaccatatc cagcccgcca
     9841 gtacgttgaa gaacgggcag ggtgaaatcc attgatgtgg caccgcataa gcccagtgca
     9901 gtagagcggc tgcggcgaat cagcccaggg atcaacataa tagcaatcag ttcacgggcc
     9961 agatcattaa aaaacgccgc gctcccgatt accggaccaa aagattcggt caataaaata
    10021 ccggaaagag aataccagcc gaaaccggag gccattgcca gcgcggtatt gatggggaga
    10081 tcaagaataa aggcgttaat taaaccacca attaatgaac tgacaaccac caccacggcg
    10141 acaatcattc cccggcgatt aaggacaatc tgctttaagg tcatgccatt attgcgcaac
    10201 tgaataccaa cgaggaaaag tagcaaaatt aacgtgtatt cactggcttc ggtcgcgtgt
    10261 tgtaagaaag ccagtccact cagaccaatg gcaaaaccaa tcactactac gccgcacagt
    10321 tttagcgact ccagcgccat cgcaatacgc gacgggagtt tttcttgctg atggtggttg
    10381 cgccacggca ggcctcgctc cagccacatc agggcggcaa tattacacag taaaataacg
    10441 gtaatactga cggcagaata atgcagaatc gccaacaggt tactggcgag gttatcgaga
    10501 aacgccagac tgatacccat aaaaaagaga ataaggtaaa ccatccagct taatagctga
    10561 ttaataactt ttaacgcagc ttgttggcga agcggaatga ggtaacctac aatcagggga
    10621 accagaatga ttaacagccc agaaaacatg aaaacccagt ccttgcaaag atgaagtcga
    10681 aatgcgcgat gacacactac tgaaagcgga aggacgagta aagttgcaat taaaaggaaa
    10741 tgttatgcat aaggagcagt agagtattcg ttttcattta aagatattct tgcgctttaa
    10801 ttacaaactg caccgatgtt ggtggcgtca aaat
//
LOCUS       DAZRUF010000142        10834 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZRUF010000142 DAZRUF010000000
VERSION     DAZRUF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.142"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..279)
                     /locus_tag="ACHXOL_002882"
     CDS             complement(<1..279)
                     /locus_tag="ACHXOL_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR6809815.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(446..1186)
                     /locus_tag="ACHXOL_002883"
     CDS             complement(446..1186)
                     /locus_tag="ACHXOL_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR6809816.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1279..1914)
                     /gene="argO"
                     /locus_tag="ACHXOL_002884"
     CDS             complement(1279..1914)
                     /gene="argO"
                     /locus_tag="ACHXOL_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR6809817.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2053..2913)
                     /gene="mscS"
                     /locus_tag="ACHXOL_002885"
     CDS             complement(2053..2913)
                     /gene="mscS"
                     /locus_tag="ACHXOL_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR6809818.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3104..4183)
                     /gene="fbaA"
                     /locus_tag="ACHXOL_002886"
     CDS             complement(3104..4183)
                     /gene="fbaA"
                     /locus_tag="ACHXOL_002886"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR6809819.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4398..5561)
                     /gene="pgk"
                     /locus_tag="ACHXOL_002887"
     CDS             complement(4398..5561)
                     /gene="pgk"
                     /locus_tag="ACHXOL_002887"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR6809820.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5611..6630)
                     /gene="epd"
                     /locus_tag="ACHXOL_002888"
     CDS             complement(5611..6630)
                     /gene="epd"
                     /locus_tag="ACHXOL_002888"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR6809821.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7002..7433
                     /locus_tag="ACHXOL_002889"
     CDS             7002..7433
                     /locus_tag="ACHXOL_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR6809822.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7456..8034
                     /locus_tag="ACHXOL_002890"
     CDS             7456..8034
                     /locus_tag="ACHXOL_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR6809823.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8035..8742
                     /locus_tag="ACHXOL_002891"
     CDS             8035..8742
                     /locus_tag="ACHXOL_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR6809824.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8730..9407
                     /locus_tag="ACHXOL_002892"
     CDS             8730..9407
                     /locus_tag="ACHXOL_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR6809825.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9401..10078
                     /locus_tag="ACHXOL_002893"
     CDS             9401..10078
                     /locus_tag="ACHXOL_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR6809826.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10050..10763)
                     /locus_tag="ACHXOL_002894"
     CDS             complement(10050..10763)
                     /locus_tag="ACHXOL_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR6809827.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10760..>10834)
                     /locus_tag="ACHXOL_002895"
     CDS             complement(10760..>10834)
                     /locus_tag="ACHXOL_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR6809828.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2581 a         2879 c         2698 g         2676 t
ORIGIN      
        1 tcgtgaacga cgccctgagt tatggatttc attatcaatc gcagaaagtc gttgataaac
       61 cgggaacagt ttttgataca gttcctgacc ggctttattg aggctgatat tattatcttt
      121 gcgggtaaat agcgtatagc caatttgttc ttccagcgcg gcaatgcttt taccaaatgg
      181 cgaagccgtc atatggattt tttctgccgc ccgggcaata ttattggttt gcgctaataa
      241 aatgaaattg cgcatctttt tcgaaataaa aatgtccatc ccccctcccc gcatccttgt
      301 ccgcttaacc gtttcagtca gcctatcctt gatgaaaccg cgagcaaaga taggtgatta
      361 cgtcatggtt ttacagaaaa ttacagaaaa aggaggcaat atcgggtaaa ggcattagcc
      421 cgacgaatac gtcgggctac aagtattatt gtgctgcagg tgttttagcg ggttgttgat
      481 ccacaggttc taactggaag accacatcga cctgatcatc aaactgaata gcggcctgct
      541 cgtaagtttc ctgggcagac accggcgcgg catcggcttt catcatccgc accattgggc
      601 tgggctgata gttggaaaca tggtagcgca cgctatatac cggccccagt ttacgatgaa
      661 agccgttcgc cagttcctgc gcctgatgaa tcgcgttatc aatcgctgcc ttacgcgctt
      721 tgtctttata ggcatccggc tgcgccacgc ccagcgacac agaacgaatt tcgttaagac
      781 ccgccttcag cgcgccatcc agcaaggaat tcagtttgtc taactgacgg agcgtgactt
      841 ccaccgttct cacagcgcgg tagcctttca ggatactttt accatcctga taatcataat
      901 ctggctgggt gcgtaagttc gctgagctga tatctttttt cgcgatctga ttgagttcaa
      961 ggaaggaaat gtattgtgcg acgcgctcat ctgcctgttt cttggctgta gcggcatcct
     1021 tcgcggccac gttaacttca atcgcaagag tggcaatgtc tggcaccgca tccacacttg
     1081 ccgtaccgga ggtgacaata tgcggtccat ccggcaattc gttagcctgc gctgccatcc
     1141 cgctaatacc cattaatgcc gccagggcga taactttgaa cttcacgtta gtctctccgt
     1201 gttaattgac ttgcccaaaa ttcaggacct ctggaggcaa gcttagcgcc tctgttttat
     1261 ttttccatca gatagcgcct aactgaacaa ggcttgtgca tgagcaatac cgtctctcgc
     1321 cagctgcaag gctataaacc acatgacaca tcccactacc agattgataa tgcgctgtga
     1381 ttttgccgtg cgcaggcgcg gtgccagcca ggctgcgaga atagccagac caaagaacca
     1441 caggaaagag gcgctaattg tcccgagcgc aaaccaacgt tttggttcga cgtcaagctg
     1501 cccgccaagg ctgcccagta caacaaaagt atccaggtaa acatgcggat tcagccaggt
     1561 cactgccagc atggtggcga taattttcca tctgccttgc ttcaggactt cggcactagc
     1621 taactcaata ttactactca tcgctgtttt aaaagcgcca aaaccatacc acagcaagaa
     1681 ggctacgccg ccccaggtga ccagcgccag caaccacggc gactgcatca ataacgcgct
     1741 gccaccaaaa atcccggcgc aaatcaggac caaatcgctg atagcacaaa gtaaggcaat
     1801 cataatgtgg tactgacgac gaatgccctg attcatcaca aaagcatttt gtggaccgag
     1861 cggcagaatc atagctgccc caagagcaat accttgaaag taataagaaa acacgttgga
     1921 tattccgaat taagtatctg atgcagagac tatatcgcgg gattgttatt agcggaaatt
     1981 gataatatta attggcaatc agaataacta ataagagaga ggccagagag tattatctgg
     2041 cctcagcgtt gattacgcag ctttgtcttc tttcacccgc ttaaagttca catccatttg
     2101 cgggtacggg aagctgatac cggcggcatc aaattcacgt ttaatacgct ccagcacatc
     2161 ccagtacacg ttttgcagat cgccgctgtt gctccagacg cggaccacga aattaatcga
     2221 cgatgcacca agttcgttca gacgcacagt catttcgcga tctttcaaaa tgcgatcttc
     2281 agactggata atattggtca ggatctgctt aacctgatcg atatcggaat catacgccac
     2341 gccaataata aattcgttac ggcgaactgg ctcgcgagag aagttaataa tatttccggc
     2401 aataatttta ccgttcggaa taacgataat tttaccgtct gcagtacgca tggtggtgga
     2461 gaaaatctgc acactcagca cagtaccggc tacgccgccg agatcaacat attctccggc
     2521 acggaacggg cggaacatga caagtaacac gccagcggcc aggttagaaa gtgacccctg
     2581 caaagccaga ccaacagcta agcctgcggc accgagtaca gcaatgactg acgctgtttg
     2641 tacacccacg cgtcccaggg cagcgattag cgtaaaggcg ataataccgt aacggactaa
     2701 tgcagaaaga aaatcagcaa cagtggcatc gattttacgg gagatcatca ggcgattcac
     2761 cgcgttggaa atcatccgcg cgataatcaa accaacgatg atgatcgcga gtgccgccac
     2821 gatgtttact gcataactta gcagcagcgc ctggttagct accagccagc ttcccgcgcc
     2881 gtttatgcta tcgacaacat tcaaatcttc cattcaatat tccttttcaa ctgactcaaa
     2941 atggagaaat acactccgcc gccttatgac gggcagtctg acaagggtaa acaataaagc
     3001 ataattttgc caaatagatc acagatttct catctgaatc accttgcttt tggcgaaaaa
     3061 aagacccgca gagcgggcct tgagataagc agaaaggaat atcttacaga acgtcgatcg
     3121 cgttcagttc ctggaatgct ttttccagac gagcgatcat cgaagtctga ccggcacgca
     3181 gccatacgcg cggatcgtag tatttcttgt tcggctgatc ttcgcctttc gggttaccca
     3241 actgcccctg cagataagct tcgttcgctt tgtagtagtt cagaacgcct tcccaggttg
     3301 cccattgggt atcggtatcg atgttcattt ttactacgcc gtagcttacg gagtctttga
     3361 tttcctgagc agtagaaccg gaaccaccgt ggaatacgaa gttcaggctg ttgtgcggca
     3421 ggttgtgttt cttggaaaca tattcctgag aatcacgcag gatggtcgga gtcagaacca
     3481 cgttacccgg cttgtaaaca ccgtgtacgt taccgaagga cgcagcgatg gtgaaacgcg
     3541 ggctgatttt gctcagttcg gtgtatgcgt aatcaacgtc ttccggctgg gtgtacagtg
     3601 cagaagcgtc catgtggctg ttgtccacgc cgtcttcttc accaccggtg caacccagtt
     3661 cgatttccag agtcatgccg attttggaca tgcgctccag gtatttagag cagatttcga
     3721 tgttctcttg cagagattct tcagacaggt cgatcatgtg agaagagaac agcggcttac
     3781 cggtagctgc gaagtgtttt tcacccgcgt ccagcagacc gtcgatccac ggcagcagtt
     3841 tcttcgcgca gtggtcagtg tgcaggataa ccggaacacc ataatgttca gccatctggt
     3901 gaacgtgatg cgcaccagag atcgcgccca ggatagcagc accctgcgga acgtcagatt
     3961 tcacgccttt accagcgata aaggaagcac caccgttgga gaactgaacg ataaccggcg
     4021 ctttaacttt agcagcggtt tccagtacgg cgttgatgga gtcagtaccg acgcagttta
     4081 ctgctggcag tgcgaagttg ttttcttttg ctacctggaa aactttctgt acgtcatcac
     4141 cagtgattac gccaggtttt acgaaatcaa aaatcttaga catgtctctt gtcctgtatc
     4201 gtcgggcctt atactcatca tacttcaagt tgcatgtgct gcgtctgcgt tcgctcaccc
     4261 cagtcactta cttatgtaag ctcctgggga ttcactctct tgtcgccttc ctgcaactcg
     4321 aattatttag agtatgaaaa aatgcgtgct ctaaaagcgc gctgaaacaa gggcaggttt
     4381 ccctgccctg tgatttttta cttcttagcg cgctcttcga gcatcgctac tgcaggcagt
     4441 actttacctt ccacgaattc gaggaatgcg ccgccgccag tggagatgta ggagattttg
     4501 tcagcaatgc cgaacaggtc gattgctgcc agagtgtcgc cgccgccagc gatggagaac
     4561 gcttcgctgt ctgcgatagc gttagccacg atttcagtac ctttgcggaa gttcgggaat
     4621 tcgaacacgc caaccggacc gttccacaga atggttttcg cattcttcag gatttcagcc
     4681 agttcctgag cggaagcatc accgatatcc aggatctgct cgtcagcttt cacatcgtta
     4741 acagatttca gggtagccga tgcagtttca gagaactcgg ttgctacgcg aacatcagac
     4801 ggaaccggga tgttgcaggt ggtcagcaga cgtttagctt cgtcaaccag gtcagcttcg
     4861 tacagggatt tacccacatc gtggccttgt gccgcgataa aggtgttagc gataccacca
     4921 ccaacaatca gctggtcagc gattttagac agggagtcca gaacggtcag tttggtagat
     4981 actttagaac caccaacgat agccaccatc gggcgagcag gttctttcag tgctttaccc
     5041 agcgcgtcca gttcagctgc caacagcggg cctgcgcacg caacatcagc gaatttaccg
     5101 ataccgtgag tagaagcctg cgcgcggtga gcagtaccga atgcgtccat tacgaacacg
     5161 tcacacagtg cagcgtattt tttggacagg gtttcgtcgt ctttcttctc gcctttgttg
     5221 aagcgaacgt tttccagaac aaccagttca ccttcagcaa cgtcaacgcc gtcgaggtaa
     5281 tctttaacca gacgaaccgg gttagacagt ttgtctttca ggtagttaac aaccggcagc
     5341 agagagaatt cttcgttgta ctcgccttcg gtaggacgac ccaggtggga agttaccatc
     5401 actttcgcgc cttgtttcag ggccagttcg atggtcggca gagaagcacg gatacgcgcg
     5461 tcgctggtta ctttcccgtc ttttactggt acgttcagat ccgcacggat aaatacacgt
     5521 ttcccagcaa gatccagatc ggtcatctta attacagaca tggtgaatcc tctcgttgat
     5581 tctaaaagtt ttgcagacgc tgcttgcgtc ttacctgaaa gcaacagtag ccatagctaa
     5641 cgtcgtgtcg agcattcggt tagcaaagcc ccattcgtta tcgcaccaga ccaacgtttt
     5701 gatcaggtgt gcgccactga cccgggtttg ggtgccatcg acaatggcac tgtgcggatc
     5761 gtggttaaaa tctacagaga ccaacggcaa ttccgtatag tcaactatac catgaaatgc
     5821 accttgtgct gctttttgca gcaacaggtt gacttcattg gcttttacag gtttcttcac
     5881 cgtcacgctt aaatcgattg ccgtcacatt tatggttggc acacgtaccg caatcgcttc
     5941 aaagcgatcg ttaaattgcg gaaaaaatcg tgtgataccg gcggccagtt tagtatcgac
     6001 cggaatgatc gactggctgg ctgcccgggt gcgacgcagg tcaggatggt atgcatcaat
     6061 aacctgttga tcgtgcatgg cggagtgaat tgtggtcaca gtgccggact caataccgta
     6121 cgcatcatct aacaatttga tgacgggaat tatgcaattc gtggtacagg aagcgttaga
     6181 aacgatgcgg tgttccgcac gaagttgatc ctgattgacg ccgtaaacaa cggtcgcgtc
     6241 gagatcgtta ctgccaggat gtgaaaagag cacttttttg gccccggcgg caatatgcgc
     6301 ttcgccatgc tcgcgggagc catatacgcc ggtgcagtcg aggactacat caacgccaag
     6361 ttcacgccag gggagcgatt gcagtgaacg ttcatgcaat acgcggatgg cgtcatcacc
     6421 aacaaaaagt tgatcgcgtt cctgtcgtac ttcccatgca aaacggccat ggctggtgtc
     6481 atatttcaac aaatgcgcca tgcccgcagc atccgccagt tcgttgattg ccaccacggt
     6541 aatttccgcc cggcgtccgg attcatacaa agcacgaacc acattacgcc cgatgcgacc
     6601 gaagccattt atcgctacgc gtacggtcat agatctcctg caaggttttc cctgagcaaa
     6661 tttgccagac agagtaatcc agcaaatcgt ccggggaaac cttacctgtc gcaaactgcg
     6721 actgattggt taattgtcga acatttaatc gactgaaacg cttcagctag gataagcgaa
     6781 acgtggaata aaaggaatga ttgtccagtc aaagaaaaca attatctgac ttacgtcacg
     6841 ttttcagccc cgcccctgtg gaatttattc cactttgcag aatcacgaat aagcgtagca
     6901 aaatcttttc gttgtgccgt cacaaaccct gacttatatc agaatatgac tgatgcagcg
     6961 tcgatattat ccgcaatacg tccgagtttc ggagtactcc aatgatgaca ttgccctacc
     7021 catattcttc ccccgcacgc ttttatgcac tgcgcctcct ccccgggcag gaagtttttt
     7081 cccagctaca cgcttttgca cagcagcatc aacttcatgc cgcctggatt gcaggctgta
     7141 ccggtagcct gactgacgtc gccttgcgct atgccgggca agaaggcacc acgctcctta
     7201 acggtacatt cgaagtgatc tcactaaacg gcacgctgga acagagtggt gagcatcttc
     7261 atctctgcgt ttccgatcca cacggcacga tgcttggcgg tcatatgatg cccggctgta
     7321 ccgtgcggac cacgcttgaa ctggtgatcg gtagccttga ggaactggcg tttagccgcc
     7381 aaccttgcgc gctttctggt tacgacgagt tgcatatttc ccccgttaaa taactacttt
     7441 caaagaggtt ttcttatggc gcgtagccac ttttccagtc aggcactggt gttgatcgtt
     7501 atatctatcg ccatcaatat gatcggcggg caactggcca gcatggtcaa attacctata
     7561 tttctcgact ccatcggcac acttattagc gcggtactgc ttggtccggt gatcggaatg
     7621 ctgaccggct tactgaccaa tttactctgg ggattgctga ccgacccgat cgccgccgca
     7681 tttgcacccg tcgcgatggt catcggcctg gtcgccggtt ggctggcgcg agcgggttgg
     7741 tttcgcaccc tgccaaaagt cgttgtcagc ggcgtgatta ttacgcttgc ggtaacggtc
     7801 gtcgctgtac cgctgcgtac tgcgctgttc ggcggtgtca ccggtagcgg agccgatttg
     7861 ttcgttgcct ggatgcactc gatggggcaa aacctggtgg agtcggtggc gattacggtg
     7921 ataggcgcga acctggtcga taaaattctt acagcggtga ttgtctggct tttactgcgc
     7981 cagctgccga ttcgcaccac gcgccatttt ccggcaatgg ctgccgtgcg ctaaatgcat
     8041 ccatttacct cattaacact gtgggcattg gcggcctgca ccacgcttat cctgcccgcg
     8101 cagaccattt tgccgatcta tagcgcggcg accttttttt gtcttattgc gctaaaggcc
     8161 acgcggcggc gggcaaaata tgtcgcctgg ctgatgttct cgctgggagc cgggctgtgg
     8221 ctggtacacg gtggctggct aacagaatgg ctaagcggca cgccgcgttc gccggagcgt
     8281 tggacgcatg ctattacgct ctggttgcgg attctggcga ttgtttcaac ctcacagttg
     8341 tggatgcagt acgtccccgt gcaatgtttt atccgcgctc tgtttgcttc tcgcctgccg
     8401 ccaggcgtcg cttatctgtt tgccggaccg ttattggtcg ttgagcagtt aaaacggcaa
     8461 ttagcgatta ttcatgaagc gcagcgtgcc cgcggcgtgc cgctggatga gggctggtat
     8521 cagcgtttac gtgcgatgcc cgcgttaatt attctgctaa cacacaatgc gcttaacgat
     8581 ctggctgtcc gtggtgcggc gctggatatg cgggcatttc ggatccacaa ccgacgaaca
     8641 acgttgtggg caccagccga tagcacccta caacgcgtag cgcgttacac catgatcttg
     8701 ctgatgctaa cagaattcgg agcgtggata tggttacgtt agaacagttc cgttattgcc
     8761 cgacacactc aacgcatcct ccgttctgtt atgactttca ctatgtcaaa ccggggatgg
     8821 tcgccatctt tggtgacaac ggcagtggca aaagtacgct ggcacagctg atggcgggct
     8881 ggtatccgga ctttctgcca ggagagataa caggcacggg gacgctcctc ggtacgccca
     8941 ttgggcatct cccgcttaat gaacaatccg ccaccatcca gctcgtccag caatcgccct
     9001 atttgcagct ctccggctgt acctttagcg tggaagaaga agttgccttt gggccagaaa
     9061 atctgtgtct tgctgaaaca gagattatgg cgcggattga tgccgccctg actctgaccg
     9121 agtgccagcc gttgcgccat cgtcacccgg caacactctc cggtggagag acacaacgcg
     9181 tggtcatcgc ctgcgccatc gcgatgcagc caaagctctt aattcttgat gaagccttta
     9241 gtcgcctgac gccgcaggcc agcgaaatgc tgttgcagcg cttacaacac tgggcgcttg
     9301 aacgtggttc gctgattatt ctttttgaac gtcatcgcac acctttcctg aactactgcc
     9361 agcaagcgtg gcaattgcaa aacggagcgc tccagccact atgttaacgt taaatcagat
     9421 atcttatcgc tggcctagcg cagcaacgga ttgtctttgc gacatttctc tgcaactcaa
     9481 acaaggtgaa tggttggcgc tgaccggcga caatggcgcg ggaaaatcga cgctgttacg
     9541 agtaatggca ggtcttctta cgcccacagc cggtacggtt atgctgcaac aacaggcgat
     9601 gaaaaacctg aaaaaccgcc agcgcgcggc aaaggttggc gtactgtttc aggaagcgga
     9661 aaaccaactt tttcacagca ccgtcgctga cgagatcgca tttggtttga aactgcaaaa
     9721 atgcccggcg gatgaaatca ctcaacgtac ccatgctgcc ctgcaatgtt gtcagttagc
     9781 agacactgcc aactcgcacc cgttggatct gcatagtgcg cagcgacgga tggtcgccgt
     9841 cgcctgcctg gaagcactct ctccgccact attactgctg gacgagccga gtcgggattt
     9901 tgacgaaaat tggttgagcg tatttgaaag ctggctggag aagtgccgcc agcgaggcac
     9961 gagcgtggta gcaatcagtc atgacgccgc atttacccgg cgtcatttct ctcgtgtggt
    10021 gcgactggaa gatggtgtaa tcaggaacgt taatccacca gacgatattc acccgtagcg
    10081 gtcatcatta aggtcaaatt tgcaggcaga ctctcttcca gcacccggcg aacattcggc
    10141 ccgtcggtac ggtcataaaa ggcttctgca tctgccagcg atacccctcc cgccagcttg
    10201 cgccccacca gacgctcccg caacgcagta gctggcgcgt tgataaagat cgagaaatca
    10261 caaaattgcg caagctggca ccacttctcg tcgtccagca acaaccagtt tccttcgacg
    10321 atgacgagtg gtgcggtaac gtgcaacgca tcttcaacag gatcatgctt ttgtcgatcg
    10381 tactgcggcc acgtacaatc cccttccacc acctggcgca gattttccgc cagtttcgca
    10441 acgtcaaatg tctctggtgc gcctttgaag gggcgcaatt gatgcgcgtc cagccagcta
    10501 ttgtaatggt gaaaaccatc catcgggagc gtctgaatag cgggcagctc cgggtcttgt
    10561 tgcacgagat attcccagaa ggtggttagg gtagatttac ccgtccccgg cggagcgcaa
    10621 agaaaaacca ccgtacgccg ctgcggattt accgtctgta aggccgccag catacgcagc
    10681 aacggtttat gcacgcgttc tatctcttca tcatggtact gcgcttgcac gttcagccca
    10741 ttcaccgtta attcaatttt cacggcgtta attcctttaa ggtggtcgtt actgccagcg
    10801 tcagcgccgt tttgaccatt ccgctaaaac gcgc
//
LOCUS       DAZRUF010000143        10801 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZRUF010000143 DAZRUF010000000
VERSION     DAZRUF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10801
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.143"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            262..666
                     /gene="sdhC"
                     /locus_tag="ACHXOL_002896"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             262..666
                     /gene="sdhC"
                     /locus_tag="ACHXOL_002896"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR6809829.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            660..1007
                     /gene="sdhD"
                     /locus_tag="ACHXOL_002897"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             660..1007
                     /gene="sdhD"
                     /locus_tag="ACHXOL_002897"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR6809830.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1007..2773
                     /gene="sdhA"
                     /locus_tag="ACHXOL_002898"
     CDS             1007..2773
                     /gene="sdhA"
                     /locus_tag="ACHXOL_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR6809831.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2789..3505
                     /gene="sdhB"
                     /locus_tag="ACHXOL_002899"
     CDS             2789..3505
                     /gene="sdhB"
                     /locus_tag="ACHXOL_002899"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR6809832.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3806..6607
                     /gene="sucA"
                     /locus_tag="ACHXOL_002900"
     CDS             3806..6607
                     /gene="sucA"
                     /locus_tag="ACHXOL_002900"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR6809833.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6622..7839
                     /gene="odhB"
                     /locus_tag="ACHXOL_002901"
     CDS             6622..7839
                     /gene="odhB"
                     /locus_tag="ACHXOL_002901"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR6809834.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7933..9099
                     /gene="sucC"
                     /locus_tag="ACHXOL_002902"
     CDS             7933..9099
                     /gene="sucC"
                     /locus_tag="ACHXOL_002902"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR6809835.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9099..9968
                     /gene="sucD"
                     /locus_tag="ACHXOL_002903"
     CDS             9099..9968
                     /gene="sucD"
                     /locus_tag="ACHXOL_002903"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR6809836.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10072..10794)
                     /gene="mngR"
                     /locus_tag="ACHXOL_002904"
     CDS             complement(10072..10794)
                     /gene="mngR"
                     /locus_tag="ACHXOL_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR6809837.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         2540 a         2799 c         3001 g         2461 t
ORIGIN      
        1 caaactatat gtaggttaat tgtaatgatt ttgtgaacag cctatactgc cgccaggtct
       61 ccggaacacc ctgcaatccc gagccaccca gcgttgtaac gtgtcgtttt cgcatctgga
      121 agcagtgttt tgcatgacgc gcagttatag aaaggacgct gtctgacccg caagcagacc
      181 ggaggaagga aatcccgacg tctccaggta acagaaagtt aacctctgtg cccgtagtcc
      241 ccagggaata ataagaacag catgtgggcg ttattcatga taagaaatgt gaaaaaacaa
      301 agacctgtta atctggacct acagaccatc cggttcccca tcacggcgat agcgtccatt
      361 ctccatcgcg tttccggtgt gatcaccttt gttgcagtgg gcatcctgct gtggcttctg
      421 ggtaccagcc tctcttcccc tgaaggtttc gagcaagctt ccgcgattat gggcagcttc
      481 ttcgtcaaat ttatcatgtg gggcatcctt accgctctgg cgtatcacgt cgtcgtaggt
      541 attcgccaca tgatgatgga ttttggctat ctggaagaaa cattcgaagc gggtaaacgc
      601 tccgccaaaa tctcctttgt tattactgtc gtgctttcac ttctcgcagg agtcctcgta
      661 tggtaagcaa cgcctccgca ttaggacgca atggcgtaca tgatttcatc ctcgttcgcg
      721 ctaccgctat cgtcctgacg ctctacatca tttatatggt cggttttttc gccaccagtg
      781 gcgaactgac atatgaagtc tggatcggtt tcttcgcctc tgcgttcacc aaagtgttca
      841 ccctgctggc gctgttttct atcttgattc atgcctggat cggcatgtgg caggtgttga
      901 ccgactacgt taaaccgctg gcattgcgcc tgatgctgca actggtgatt gtcgttgcac
      961 tggtggttta cgtgatttat ggattcgttg tggtgtgggg tgtgtgatga aattgccagt
     1021 cagagaattt gatgcagttg tgattggtgc cggtggcgca ggtatgcgcg cggcgctgca
     1081 aatttcccag agcggccaga cctgtgcgct gctctctaaa gtcttcccga cccgttccca
     1141 taccgtttct gcgcaaggcg gcattaccgt tgcgctgggt aatacccatg aagataactg
     1201 ggaatggcat atgtacgaca ccgtaaaagg gtcggactat atcggtgacc aggacgcgat
     1261 tgaatatatg tgtaaaaccg ggccggaagc gattctggaa ctcgaacaca tgggcctgcc
     1321 gttctcgcgt cttgatgatg gtcgtatcta tcaacgtccg tttggcggtc agtcgaaaaa
     1381 cttcggcggc gagcaggcgg cacgcactgc ggcggcggct gaccgtaccg gtcacgcact
     1441 gttgcacacg ctttatcagc agaacctgaa aaaccacacc accattttct ccgagtggta
     1501 tgcgctggat ctggtgaaaa accaggatgg cgcggtggtg ggttgtaccg cactgtgcat
     1561 cgaaaccggt gaagtggttt atttcaaagc ccgcgctacc gtgctggcga ctggcggggc
     1621 agggcgtatt tatcagtcca ccactaacgc ccacattaac actggcgacg gtgtcggcat
     1681 ggctatccgc gcaggcgtac cggtgcagga tatggaaatg tggcaattcc acccgaccgg
     1741 cattgccggt gcgggcgtac tggtcaccga aggttgccgt ggtgaaggcg gttatctgct
     1801 gaacaaacat ggcgaacgct ttatggagcg ttatgcgccg aacgccaaag acctggcggg
     1861 ccgtgatgtg gtggcgcgtt ccatcatgat tgaaatccgt gaaggtcgcg gctgtgatgg
     1921 tccgtggggg ccacacgcga aactgaaact cgatcacctg ggtaaagaag ttctcgaatc
     1981 ccgtctgccg ggtatcctgg agctttcccg tacctttgct cacgtcgatc cggtgaaaga
     2041 gccgattccg gttatcccaa cctgtcacta catgatgggc ggtattccga ccaaagtgac
     2101 cggtcaggcg ctgacggtga acgagaaagg cgaagatgtg gttgttccgg ggctgtttgc
     2161 cgttggtgaa atcgcttgtg tatcggtaca cggcgctaac cgtctgggcg gcaactcgct
     2221 gctggacctg gtggtctttg gtcgtgcggc aggtctgcat ctgcaagagt ctatcgccga
     2281 acagggcgca ttgcgcgatg ccagcgagtc tgatgttgaa gcatctctgg atcgtctgaa
     2341 ccgctggaac aataatcgta acggtgaaga tccggtggcg attcgtaaag cgctgcaaga
     2401 atgtatgcag cataacttct cggtcttccg tgaaggtgat gcgatggcga aagggcttga
     2461 gcagttgaaa gtgatccgcg agcgtctgaa aaatgcccgt ctggatgaca cttccagcga
     2521 gttcaacacc cagcgcgttg agtgcctgga actggataac ctgatggaaa cggcgtatgc
     2581 aacggctgtt tctgccaact tccgtaccga aagccgtggc gcacatagcc gcttcgactt
     2641 cccggatcgt gatgatgaaa actggctgtg ccactccctg tatctgccag agtcggaatc
     2701 catgacgcgc cgaagcgtca acatggaacc gaaactgcgc ccggcattcc cgccgaagat
     2761 tcgtacttac taatgcggag acaggaaaat gagactcgag ttttcaattt atcgctataa
     2821 cccggatgtt gatgatgctc cgcgtatgca ggattacacc ctggaagcgg aagaaggtcg
     2881 cgacatgatg ctgctggatg cgcttatcca gctgaaagag aaagatccca gcctgtcgtt
     2941 ccgccgctcc tgccgtgaag gtgtgtgcgg ttccgacggt ctgaacatga acggcaagaa
     3001 tggtctggcc tgtattaccc cgatttcggc actcaaccag ccgggcaaga agattgtgat
     3061 tcgcccgctg ccaggtttac cggtgatccg cgatttggtg gtagacatgg gacaattcta
     3121 tgcgcaatat gagaaaatta agccttacct gttgaataat ggacaaaatc cgccagctcg
     3181 cgagcattta cagatgccag agcagcgcga aaaactcgac gggctgtatg aatgtattct
     3241 ctgcgcatgt tgttcaacct cttgtccgtc tttctggtgg aatcccgata agtttatcgg
     3301 cccggcaggc ttgttagcgg catatcgttt cctgatcgat agccgtgata ccgagactga
     3361 cagccgcctc gacggtttga gcgatgcatt cagcgtattc cgctgtcaca gcatcatgaa
     3421 ctgcgtcagt gtatgtccga aggggctgaa cccgacgcgc gccatcggcc atattaagtc
     3481 gatgttgttg caacgtaatg cgtaaaccgt aggcctgata agacgcgcaa gcgtcgcatc
     3541 aggcaaccag tgccggatgc ggcgtgaacg ccttatccgg cctacaagtc attacccgta
     3601 ggcctgataa gcgcagcgca tcaggcgtaa caaagaaatg caggaaatct ttaaaaactg
     3661 cccctgacac taagacagtt tttaaaggtt ccttcgcgag ccactacata gacaagagct
     3721 cgcaagtgaa ccccggcacg cacatcactg tgcgtggtag tatccacggc gaagtaagca
     3781 taaaaaagat gcttaaggga tcacgatgca gaacagcgct ttgaaagcct ggttggactc
     3841 ttcttacctc tctggcgcaa accagagctg gatagaacag ctctatgaag acttcttaac
     3901 cgatcctgac tcggttgacg ctaactggcg ttcgacgttc cagcagttac ctggtacggg
     3961 agtcaaaccg gatcaattcc actctcaaac gcgtgaatat ttccgccgcc tggcgaaaga
     4021 cgcttcacgt tactcttcaa cgatctccga ccctgacacc aatgtgaagc aggttaaagt
     4081 cctgcagctc attaacgcat accgcttccg tggtcaccag catgcgaatc tcgatccgct
     4141 gggactgtgg cagcaagata aagtggccga tctggatccg tctttccacg atctgaccga
     4201 agcagacttc caggagacct tcaacgtcgg ttcatttgcc agcggcaaag aaaacatgaa
     4261 actcggcgag ctgctggaag ccctcaagca aacctactgt ggcccgattg gtgccgagta
     4321 tatgcacatt accagcaccg aagaaaaacg ctggatccaa cagcgtatcg agtctggtcg
     4381 cgcgactttc aatagcgaag agaaaaaacg cttcttaagc gaactgaccg ccgctgaagg
     4441 ccttgaacgt tacctcggcg caaaattccc tggcgcaaaa cgcttctcgc tggaaggcgg
     4501 tgacgcgtta atcccgatgc ttaaagagat gatccgccac gctggcaaca gcggcacccg
     4561 cgaagtggtt cttgggatgg cgcaccgtgg tcgtctgaac gtgctggtga acgtgctggg
     4621 taaaaaaccg caagacttgt tcgacgagtt tgccggtaaa cataaagaac acctcggcac
     4681 gggtgacgtg aaataccaca tgggcttctc gtctgacttc cagaccgatg gcggcctggt
     4741 gcacctggcg ctggcgttta acccgtctca ccttgagatt gtaagcccgg tagttatcgg
     4801 ttctgttcgt gcccgtctgg acagacttga tgagccgagc agcaacaaag tgctgccaat
     4861 caccatccac ggtgacgccg cagtgaccgg gcagggcgtg gttcaggaaa ctctgaacat
     4921 gtcgaaagcg cgtggttatg aagttggcgg tacggtacgt atcgttatca acaaccaggt
     4981 tggtttcacc acctctaatc cactggatgc tcgttctaca ccgtactgta ctgatatcgg
     5041 taagatggtt caggcaccga ttttccacgt taacgcggat gatccggaag ccgttgcctt
     5101 tgtgacccgt ctggcgctcg atttccgtaa cacctttaaa cgtgatgtct tcatcgacct
     5161 ggtgtgctac cgccgtcacg gccacaacga agccgacgag ccgagcgcaa cccagccgct
     5221 gatgtatcag aaaatcaaaa aacatccgac accgcgcaaa atctacgctg acaagctgga
     5281 gcaggaaaaa gtggcgacgc tggaagatgc caccgagatg gttaacctgt accgcgatgc
     5341 gctggatgct ggcgattgcg tagtggcaga gtggcgtccg atgaacatgc actctttcac
     5401 ctggtcgccg tacctcaacc acgaatggga cgaagagtac ccgaacaaag ttgagatgaa
     5461 gcgcctgcag gagctggcga aacgcatcag cacggtgccg gaagcagttg aaatgcagtc
     5521 tcgcgttgcc aagatttatg gcgatcgcca ggcgatggct gccggtgaga aactgttcga
     5581 ctggggcggt gcggaaaacc tcgcttacgc cacgctggtt gacgaaggca ttccggttcg
     5641 cctgtcgggt gaagactccg gtcgcggtac cttcttccac cgccacgcgg tgatccacaa
     5701 ccagtctaac ggttccactt acacgccgtt gcaacacatc cataacgggc agggcgcgtt
     5761 ccgcgtctgg gactccgtac tgtctgaaga agcagtgctg gcgtttgaat acggttatgc
     5821 caccgcagaa ccacgcactc tgaccatctg ggaagcgcag ttcggtgact tcgccaacgg
     5881 tgcgcaggtg gttatcgacc agttcatctc ttctggcgaa cagaaatggg gccggatgtg
     5941 tggtctggtg atgttgctgc cgcacggtta cgaagggcag gggccggagc actcctccgc
     6001 gcgtctggaa cgttatctgc aactttgtgc tgagcaaaac atgcaggttt gcgttccgtc
     6061 taccccggca caggtttacc acatgctgcg tcgtcaggcg ctgcgcggga tgcgtcgtcc
     6121 gctggtagtg atgtcgccga aatccctgct gcgtcatccg ctggcggttt ccagcctcga
     6181 agaactggcg aacggcacct tcctgccagc catcggtgaa atcgacgagc ttgatccgaa
     6241 gggcgtgaag cgcgtagtga tgtgttctgg taaggtttat tacgacctgc tggaacagcg
     6301 tcgtaagaac aatcaacacg atgtcgccat tgtgcgtatc gagcaactct acccgttccc
     6361 gcataaagcg atgcaggaag tgttgcagca gtttgctcac gtcaaggatt ttgtctggtg
     6421 ccaggaagag ccgctcaacc agggcgcatg gtactgcagc cagcatcatt tccgtgaagt
     6481 gattccgttt ggggcttctc tgcgttatgc aggccgcacg gcctccgcct ctccggcggt
     6541 agggtatatg tccgttcacc agaaacagca acaagatctg gttaatgacg cgctgaacgt
     6601 cgaataaata aaggatacac aatgagtagc gtagatattc tggtccctga cctgcctgaa
     6661 tccgtagccg atgccaccgt cgcaacctgg cataaaaaac ccggcgacgc agtcgtacgt
     6721 gatgaagtgc tggtagaaat cgaaactgac aaagtggtac tggaagtacc ggcatcagca
     6781 gacggcattc tggatgcggt tctggaagat gaaggtacaa cggtaacgtc tcgtcagatc
     6841 cttggtcgcc tgcgtgaagg caacagcgcc ggtaaagaaa ccagcgccaa atctgaagag
     6901 aaagcgtcca ctccggcgca acgccagcag gcgtctctgg aagagcaaaa caacgatgcg
     6961 ttaagcccgg cgatccgtcg cctgctggct gaacacaatc tcgacgccag cgccattaaa
     7021 ggcaccggtg tgggtggtcg tctgactcgt gaagatgtgg aaaaacatct ggcgaaagcc
     7081 ccggcgaaag agtctgctcc ggcagcggct gctccggcgg cgcaaccggc tctggctgca
     7141 cgtagtgaaa aacgtgtccc gatgactcgc ctgcgtaagc gtgtggcaga gcgtctgctg
     7201 gaagcgaaaa actccaccgc catgctgacc acgttcaacg aagtcaacat gaagccgatt
     7261 atggatctgc gtaagcagta cggtgaagcg tttgaaaaac gccacggcat ccgtctgggc
     7321 tttatgtcct tctacgtgaa agcggtggtt gaagccctga aacgttaccc ggaagtgaat
     7381 gcttctatcg acggtgatga cgtggtttac cacaactatt tcgacgtcag catggcggtt
     7441 tccacgccgc gcggcctggt gacgccggta ctgcgtgatg tcgataccct cggcatggca
     7501 gacatcgaga agaaaatcaa agagctggca gttaaaggcc gtgacggcaa gctgacggtt
     7561 gaagatctga ccggtggtaa cttcaccatc accaacggtg gtgtgttcgg ttccctgatg
     7621 tctacgccga tcatcaaccc gccgcagagc gcaattctgg gtatgcacgc tatcaaagat
     7681 cgtccgatgg cggtgaatgg tcaggttgag atcctgccga tgatgtacct ggcgctgtcc
     7741 tacgatcacc gtctgatcga tggtcgcgaa tccgtgggct tcctggtaac gatcaaagag
     7801 ttgctggaag atccgacgcg tctgctgctg gacgtgtagt agtttaagct tcacctgcac
     7861 tgtagaccga cgtggatagg gtgtcggtct acggtttaaa agataacgat tactgaagga
     7921 tggacagaac acatgaactt acatgaatat caggcaaaac aactttttgc ccgctatggc
     7981 ttaccagcac cggtgggtta tgcctgtact actccgcgcg aagcagaaga agccgcttca
     8041 aaaatcggtg ccggcccgtg ggtagtgaaa tgtcaggttc acgctggtgg ccgcggtaaa
     8101 gcgggcggtg tgaaagttgt aaacagcaaa gaagacatcc gtgcttttgc agaaaactgg
     8161 ctcggcaagc gtctggtaac gtatcaaaca gatgccaatg gccaaccggt taaccagatt
     8221 ctggttgaag cagcgaccga tatcgctaaa gagctgtatc tcggtgccgt tgtagaccgt
     8281 agttcccgtc gtgtggtctt tatggcctcc accgaaggcg gcgtggaaat cgaaaaagtg
     8341 gcggaagaaa ctccgcacct gatccacaaa gttgcgcttg atccgctgac tggcccgatg
     8401 ccgtatcagg gacgcgagct ggcgttcaaa ctgggtctgg aaggtaaact ggttcagcag
     8461 ttcaccaaaa tcttcatggg cctggcgacc attttcctgg agcgcgacct ggcgttgatc
     8521 gaaatcaacc cgctggtcat caccaaacag ggcgatctga tttgcctcga cggcaaactg
     8581 ggcgctgacg gcaacgcact gttccgccag cctgatctgc gcgaaatgcg tgaccagtcg
     8641 caggaagatc cgcgtgaagc acaggctgca cagtgggaac tgaactacgt tgcgctggac
     8701 ggtaacatcg gttgtatggt taacggcgca ggtctggcga tgggtacgat ggacatcgtt
     8761 aaactgcacg gcggcgaacc ggctaacttc cttgacgttg gcggcggcgc aaccaaagaa
     8821 cgtgtaaccg aagcgttcaa aatcatcctc tctgacgaca aagtgaaagc cgttctggtt
     8881 aacatcttcg gcggtatcgt tcgttgcgac ctgatcgctg acggtatcat cggcgcggta
     8941 gcagaagtgg gtgttaacgt accggtcgtg gtacgtctgg aaggtaacaa cgccgaactc
     9001 ggcgcgaaga aactggctga cagcggcctg aatattattg cagcaaaagg tctgacggat
     9061 gcagctcagc aggttgttgc cgcagtggag gggaaataat gtccatttta attgataaaa
     9121 acaccaaggt tatctgccag ggctttactg gtagccaggg gactttccac tcagaacagg
     9181 ccattgcata cggcactaaa atggttggcg gcgtaacccc aggtaaaggc ggtactaccc
     9241 acctcggcct gccggtgttc aacaccgtgc gtgaagctgt tgctgctact ggcgctaccg
     9301 cttctgttat ctacgtacca gcaccgttct gcaaagactc cattctggaa gctattgatg
     9361 caggcatcaa actgattatc accatcactg aaggcatccc gacgctggat atgctgactg
     9421 tgaaagtgaa actggacgaa gccggcgttc gtatgatcgg cccgaactgc ccgggcgtta
     9481 tcactccggg tgaatgcaaa atcggtatcc agccgggtca cattcacaaa ccgggtaaag
     9541 tgggtatcgt ttcccgttcc ggtacactga cctatgaagc ggttaaacag accacggatt
     9601 acggtttcgg tcagtcgacc tgtgttggta tcggcggtga cccgatccca ggctctaact
     9661 ttatcgacat cctcgaaatg ttcgaaaaag atccgcagac cgaagcgatc gtgatgatcg
     9721 gtgagatcgg cggtagcgct gaagaagaag cggcagcgta catcaaagag cacgttacca
     9781 agccagttgt aggttacatc gctggtgtga ctgcgccgaa aggcaaacgt atgggccacg
     9841 cgggtgcgat cattgctggt gggaaaggga ctgcggatga gaaattcgct gctctggaag
     9901 ccgcaggcgt gaaaaccgtt cgcagcctgg cggatatcgg tgaagcactg aaaactgttc
     9961 tgaaataaat atctgtaata agaaatagcc ctcgccgctt ccctctacag gaatggcgaa
    10021 gggctgtcgg tttcgacatg gttggccatc gtatgatggc cttttttgtg cttatcgcga
    10081 tgattttcgc tgcgctatca gggtaaattt atagtcatcg gtattaaaag cgttgcggct
    10141 atattcaaac acccgaccat caactaaata tccacgcgat actttttcaa gaatcggctt
    10201 tgtctggctg atattaagca gacggctcat ctcttcggtt ggcatcagag gaatgatttc
    10261 ctgttcgcta cgatcgataa ccatcttctt cacttcttca ataaagtgat atttcgaatt
    10321 ttccatgacc tgccaggtga gatccgggaa caacgcaagc ggcatccagg tttcttccag
    10381 cgccattggc ttttgcttgc gatagcgcac gcgcttcaca tgccacacac gatcctgcgc
    10441 ggtgatttgt agctgttgct gaagaaaatc gtcagccgga atcacttcaa atatcaggat
    10501 ttcactgtgt gtatcgacgt gacggtccga cagtttttca tcaaaactgg ttaactgaaa
    10561 aatatcgtaa ttgacccgct cttctttgac gtaagtcccg ctgccctgaa tgctttcgag
    10621 gatctgctgc tcgactagct ggcgcaaagc ctgacgcacc gtaacccggc tgacgccaaa
    10681 ctctgtttgt agcgctgatt cagtgggtaa cgcatcgcca ggtttaagct cgccacgcgc
    10741 aatttgttca cgaatgcgat cggcaatctg ccggtataag ggcttgtgtc ccatttttag
    10801 t
//
LOCUS       DAZRUF010000144        10789 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZRUF010000144 DAZRUF010000000
VERSION     DAZRUF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.144"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..631
                     /locus_tag="ACHXOL_002905"
     CDS             <1..631
                     /locus_tag="ACHXOL_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFR6809838.1"
                     /translation="KQGVGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELK
                     TSKNTRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYD
                     LFERAEQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDG
                     RGQQARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(581..1774)
                     /locus_tag="ACHXOL_002906"
     CDS             complement(581..1774)
                     /locus_tag="ACHXOL_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6809839.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1910..3634
                     /gene="iucA"
                     /locus_tag="ACHXOL_002907"
     CDS             1910..3634
                     /gene="iucA"
                     /locus_tag="ACHXOL_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR6809840.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3635..4582
                     /gene="iucB"
                     /locus_tag="ACHXOL_002908"
     CDS             3635..4582
                     /gene="iucB"
                     /locus_tag="ACHXOL_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR6809841.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4582..6324
                     /gene="iucC"
                     /locus_tag="ACHXOL_002909"
     CDS             4582..6324
                     /gene="iucC"
                     /locus_tag="ACHXOL_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR6809842.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6321..7658
                     /gene="iucD"
                     /locus_tag="ACHXOL_002910"
     CDS             6321..7658
                     /gene="iucD"
                     /locus_tag="ACHXOL_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR6809843.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7664..9859
                     /gene="iutA"
                     /locus_tag="ACHXOL_002911"
     CDS             7664..9859
                     /gene="iutA"
                     /locus_tag="ACHXOL_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR6809844.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10410..10553)
                     /locus_tag="ACHXOL_002912"
     CDS             complement(10410..10553)
                     /locus_tag="ACHXOL_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809845.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2671 a         2732 c         2815 g         2571 t
ORIGIN      
        1 aaaacaagga gttggtcaca aaaaacataa agatgaaaga aacttatttc ttcatcctca
       61 actcgtcatc agtgagtcag gtgtttgcct cggtgtttat gatgactatc agtggttcag
      121 ggatgaatta aaaaccagta aaaatacccg acaagaaatc tgcaatgatt tacttcataa
      181 aaaacatgtc agtgaaaaag aaacctggcg ttgggttgaa ggatataata aagcaacaga
      241 actggctcgc caatgccctg atacccatgt attgtcaatt tctgatcgtg aaggcgattt
      301 ttatgacctg tttgaacgcg cagagcagac tcccggaata aaagctgact ggcttgtcag
      361 aatgaaattt aataacagag caacgctgaa tattaatggt aaacgggatc ataggttgtt
      421 gcacgaaaga atcatggaga ttacaccgca gcaactggtt gaattcacta tacctgatgg
      481 gcgggggcag caagccagaa cactgcctga catgctttca tcagacgcag gtcctctctc
      541 atatcctctg atttactcca gtaaggtgat gatcatatct ttatttgata gtacggtaat
      601 gccagataaa tacagacaca atggtaaata atatagttgc aaataatgct gccataatca
      661 gcgcgattgc gacacctgtt ttcgatacca gcggcaccag caaagcgcac agtccatagc
      721 caagtgtgtg actggtagcg agccagcccg cgcctgcgcc atcagccagc ttatcattca
      781 gtagaagttg ataagcgggt gttgccagag ctgctccaaa tgacagcact gcacagcccg
      841 ggtaaaacaa ccatagctgt tcggagagca tgatagccag accaccactc atcaacactc
      901 cggcactcag gagcagggcc accggagtca ggcgctgcgg acggagtacc ccgaactgcg
      961 cgataagcgc agctactgcg gacagaccca acagccaggc cacctgttgg ctaatggcgg
     1021 tggtatccgt gacgaactgg cgagtaaggg caggcgaaag tccaagctgc atcatgctca
     1081 ccgccgcagc cagtaatatt gcgcaaagca gataaggcag acaatcccgc ttcagactga
     1141 cgctcttgca ctccggtgtg ggctgtggtg gtgtgccggg caaccgcagc agcatcagca
     1201 gcgccagcac tggtgctgcc atcagtagcc ccagtggcgc cagaggatga atggccagca
     1261 ttgctgccgc gcacagcggc ccgaatagcc tgccgcaact caggccggag ctgatggttg
     1321 ccagagcggc catgcgattc ccttctccag ctctctgcaa tgcccagacc tgacaggctg
     1381 gcaccattgc cgacacggtc agcccgtagg cgatccgggc gatgattaat attcccagtc
     1441 ccaccgctgt tgttatggcg ctggtagcca tcagcacgct tcccagccct agcagggtaa
     1501 agcttatgcc atatcctccc agagcccaca acaccacagg tttactgcca gtacgggaaa
     1561 tttgctttcc ccaccacggg gacgatggca gaaacagcat tgagccaagc atcagcaggg
     1621 cagcccatac agacagactc agatttgtct gtatgaccag aacagggagc acaaccagca
     1681 gaccgttctg tccgataccg agaagcccgg cactgaacgc aagtggccag caggacagtg
     1741 ttttttgggg cgtatcttcg atccctggtg acattataat gtttcaactc catgtattaa
     1801 ttgtgtttat ttgtaaaatt aatttatctg acaataacat ttattgtcga taatgagaat
     1861 cattatcgac ataattgtta ttattttact gtgtgggagc tgtttgacta tgaccctgcc
     1921 ctctgaaaaa ccagccacag atgtggctgc gcagtgcttc ctgaatgcac tgattcgtga
     1981 aaccacagac tggaaactga cagaataccc gccagacgaa ttgctcatcc cgctggatga
     2041 gcagaaatcg ctccatttca gagtggctta tttctcccca acccagcatc accgctttgc
     2101 atttcctgca cgtctggtca cggcatcagg cagttatcct gtcgacttta ccactctctc
     2161 ccggcttatt attgataagc tacgccatca actgtttctg cccgttcccc tctgcgaaac
     2221 tttccaccag cgcgtgctgg aaagccatgt ccatacgcaa caggcaattg atgcccgtca
     2281 tgactgggcc gctctgcgtg aaaaagcgtt gaattttggc gaggctgagc aggcgctgct
     2341 gacaggacac gctttccacc ctgcgcctaa gtctcatgaa ccgtttaacc gacgggaggc
     2401 tgaacgctac ctgcctgata tggcacccca cttcccactg cggtggtttt cggtggataa
     2461 aacgcaaatc gccggtgaaa gtttgcatct caaccttcaa cagcggctga cgcgatttgc
     2521 cgcagagaat gcgcctcagt tactcaacga attaagtgac aaccaatggc tgttcccgtt
     2581 gcacccgtgg cagggggaat atcttttgca gcaggggtgg tgccaggcac ttgttgctaa
     2641 agggctgatt aaagacttag gtgaggccgg cacgtcgtgg ctgccgacca cctcttcccg
     2701 ttccctctac tgtgccacca gccgcgatat gatcaagttc tccctgagcg tacggctgac
     2761 caactccatc cgtactctgt ccgtgaaaga agtgaagcgt gggatgcgcc tggcacgcct
     2821 ggctcaaacc gacggctggc agatgctaca ggtccgcttc ccgactttcc gggtaatgca
     2881 ggaggatggc tgggccgggc tgctcgatct taacggcaac atcatgcagg aaagtctgtt
     2941 tgccctgcgt gaaaatctgc tggtggatca gccgaaaagc cagactaacg tactggtctc
     3001 cctgactcag gccgcacctg atggcggtga ttcgctgctg gtttcggcgg taaaacgcct
     3061 gagcgatcgc ctcggtatca ctgtgcaaca ggccgcccat gcatgggtcg atgcatactg
     3121 tcagcaagtg ctaaagccgc tgtttacggc tgaagcggat tacggcctgg tgctgctggc
     3181 gcatcagcaa aatattcttg tccagatgct tggggatctg ccggtcggat ttatttaccg
     3241 tgactgtcag ggcagcgctt ttatgcctca cgcgacagac tggctcgatt ctattggcga
     3301 ggcgcaggcg gaaaatattt tcacccatga gcagttgctg cgctatttcc cttattacct
     3361 gctggttaac tccacttttg ctgtgaccgc tgcgctgggg gctgccgggc tggacagcga
     3421 atcgaatctg atggctcgtg tacgagcatc gctggctgaa gtgcgtgatc aggtgactca
     3481 taaaacctgt ctcaactacg tgctggaaag tccgtactgg aacgtaaaag gtaacttttt
     3541 ctgttatctg aacgatcata acgagaacac cattgttgac ccttcggtga tctatttcga
     3601 tttcgctaac ccgctgcagg ctcaggaggt ctgaatgtct gaggcaaaca ttattcacag
     3661 cagatatgga ctgcgctgtg aaaaactcga caagcccctg aatcttggct ggggactgga
     3721 caatagcgcg gtgttgcact gtcccgggga gctgccgaca gggtggctgt gcgatgcgct
     3781 ggatcagata tttatcgccg caccacaact gtcagcagtg gctctcccct gggccgaatg
     3841 gcgtgaggag ccacaggcgc tgacgctttt tgggcaggta aaaagcgaca ttatccatcg
     3901 tactgccttc tggcagttac cgttatggtt gagttcaccg gcaaaccggg cctccggtga
     3961 aatggttttt gatgcagagc gtgagattta tttcccgcag cgcccacccc gtccacaggg
     4021 tgaggtttac cgccgttacg atccacgtat tcgcaggatg ctgagtttcc gcattgccga
     4081 tcccgtttct gatgcagaac gtttcacccg ctggatgaat gatccgcgcg ttgagtattt
     4141 ctgggagcaa agtggctcac tggaggtaca gaccgcctat ctggagcgcc agctcaccgg
     4201 taaacatgca ttcccgctga ttgggtgctt cgacgatcgg ccattcagct atttcgaaat
     4261 ctactgggcg gcggaagacc gcattggtcg tcactattcg tggcagccct ttgaccgtgg
     4321 cctgcatctg ctggttggtg aacagcaatg gcgcggggcc cactatgtgc aaagctggct
     4381 gcgcgggctg acacattacc tgctgctgga tgagccccgt acgcagcgca cagtactgga
     4441 gccacgcacc gataaccagc gcctgttccg ccatcttgag cctgcgggat accggacaat
     4501 taaagagttc gacttcccac ataagcgctc gcgcatggtg atggcggatc gtcatcactt
     4561 cttcacggag gtcggtctgt aatgaatcac aaggactggg attttgttaa ccgtcggctg
     4621 gtggcaaaaa tgttgtctga gatggagtat gagcaggttt tccacgcgga atctcagggc
     4681 gatgaccact actgcattaa cctgccggga gcacaatggc gcttcatcgc tgaacgtggt
     4741 atctggggct ggctgtggat tgatgcccaa acgttgcgct gcacggacga accagtactg
     4801 gcgcagacgc tgctgatgca gctaaagccg gtgctgtcaa tgagtgatgc cactgttgca
     4861 gagcatatgc aggatttgta tgccacgcta ctgggcgacc tgcaactgct gaaggcccgt
     4921 cgcggactga gtgccagtga cctgattaat cttgatgccg atcgtctgca atgcctgctg
     4981 agcggtcatc ctaaattcgt ttttaataaa ggtcgccgtg gctggggcaa agaggcgctg
     5041 gaacgatatg cgccagagta taccaacacc ttcagactgc actggctggc ggtaaaacgt
     5101 gaacatatga tctggcgctg cgataacgat ctggatattc agcagttgtt gactgccgca
     5161 atggatccgc aggagtttac acgcttcagt caggtctggc aggaaaacgg gctggatcac
     5221 aactggctac cgctgccggt acatccgtgg cagtggcagc aaaaaatcgc cacagacttc
     5281 atcgctgatt ttgtcgaagg caggatggtg tctctcggcg agtttggcga ccagtggctc
     5341 gcccagcagt cgctgcgtac cctgaccaac gccagccgcc ggggagggct ggatatcaag
     5401 ctgccgctga ccatctacaa cacctcatgc taccggggaa tccctggcag atatatcgct
     5461 gccggaccac tggcttcacg ctggctgcaa caggtttttg cgaccgacgc caccctggtg
     5521 caaagcggcg cagtgatcct tggtgaaccg gctgcaggct atgtgtccca tgaaggctat
     5581 gccgcgcttg cccgggctcc ctatcgctac caggaaatgc ttggtgttat ctggcgggag
     5641 aatccgtgcc gctggctgaa accggatgaa agtcctgttc tgatggcaac actgatggag
     5701 tgcgacgaaa acaatcagcc gctggcaggc gcatatatcg accgctccgg actggacgct
     5761 gaaacctggc ttacgcaatt gttccgggtg gttgtggttc cgctgtatca cctgctttgc
     5821 cgctacggtg tcgcgcttat tgcacacgga caaaatataa cgctcgccat gaaagagggg
     5881 gtaccacagc gtgttctgct gaaagacttc cagggcgata tgcggctggt gaaagaagcg
     5941 tttcccgaaa tggactcttt gcctcaggag gtgcgtgatg ccacatcccg tctgagtgcg
     6001 gactacttaa tccatgattt gcagacgggt cacttcgtga cagtactgcg ttttatttcg
     6061 ccactgatgg ttcgtcttgg cgtacctgaa aggcgatttt atcaactgct ggcagcagtg
     6121 ttgagtgatt acatgaacaa acatccacaa atggcagagc gttttgcgct tttctcactc
     6181 ttcaggccac aaatcattcg cgtggtgctg aacccggtaa aactgacctg gccggacctg
     6241 gatggcggca gtcgcatgct gccgaattac cttgagaatc tgcaaaatcc gctgtggctg
     6301 gtaactcagg aatatgaatc atgaaaaaaa gtgtcgattt tattggtgtc gggacagggc
     6361 catttaatct cagcattgct gcattgtcac atcagatcga agaactgaac tgtctcttct
     6421 ttgacgaaca tcctcatttt tcctggcatc cgggtatgct ggtaccggat tgtcatatgc
     6481 agaccgtctt tctgaaagat ctggtcagtg cagttgcacc tacaaatccc tacagctttg
     6541 ttaactatct ggtgaagcac aaaaagttct atcgcttcct tacaagcagg ctacgtacag
     6601 tatcccgtga agagttttct gactatctcc gctgggctgc tgaagatatg aataacctgt
     6661 atttcagtca taccgttgaa aatattgatt tcgacaaaaa aagtcgattg tttctggttc
     6721 agaccagtcg gggagaatat tttgcccgca atatctgcct tggtacagga aaacaacctt
     6781 atttaccacc ctgtgtgaag catgtgacac aatcctgttt ccatgccagt gaaatgaatc
     6841 ttcgtcggcc ggacctgagt ggaaaacgga taaccgtggt tggtggagga cagagtggtg
     6901 cagacctgtt ccttaatgca ttacgcgggg aatggggaga agcggcggaa ataaactggg
     6961 tctcacggcg taataatttt aacgcactgg atgaggctgc ttttgctgat gagtatttta
     7021 cacctgaata tatttcaggc ttctccggac tgaaggaaga tattcgccat cagttactgg
     7081 atgagcagaa aatgacatcg gatggcatca ctgccgattc tttactgacc atttatcgtg
     7141 agttgtacca ccgttttgaa gttctgagaa aaccaagaaa tatccgtctg ctacccagtc
     7201 gctcggtaac aaccctggaa agtagtggtc cgggctggag gttattgatg gaacatcatc
     7261 tggatcaggg cagagagagc ctggaaagtg atgtggtgat tttcgccaca ggttaccgtt
     7321 ctgcattgcc acaaatactt ccctcactga tgcccttgat caccatgcac gataagaaca
     7381 cctttaaagt gcgtgatgac ttcactctgg aatggagtgg cccgaaagaa aataacatct
     7441 ttgcggtcaa cgccagcatg caaactcatg gcatcgccga accccagctc agcctgatgg
     7501 cctggcgatc tgcacgtatt cttaatcgcg tactggggcg tgatttattc gatctcagta
     7561 tgccgcccgc actgattcag tggcgcagcg gcagccggaa aaaaccgcag ccggaggctg
     7621 cttctttaac tcactataca gcaaatattc aggaataacg atgatgcgca aaaagtatat
     7681 gccccgggct cttggtccgc tgcttcttgt cgtgctgtca ccagctgtcg cccagcaaaa
     7741 cgatgataat gagatcatag tgtctgccag ccgcagcaat cgaactgtag cggagatggc
     7801 gcaaaccacc tgggttatcg aaaatgccga actggagcag cagattcagg gcggtaaaga
     7861 gctgaaagac gcactggctc agttaatccc cggccttgat gtcagcagcc agagtcgaac
     7921 caactacggt atgaacatgc gtggccgccc gctggttgtc ctgattgacg gtgtgcgcct
     7981 caactcttca cgttccgaca gccgacaact ggactctgtc gatcctttta atatcgacca
     8041 tattgaagtg atctccggcg cgacggccct gtacggtggc gggagtaccg gagggttgat
     8101 caacatcgtg accaaaaaag gtcagccgga aaccatgatg gagtttgagg ctggcacaaa
     8161 aagtggcttt aacagcagta aagatcacga tgagcgcatt gccggtgctg tctccggcgg
     8221 aaatgaccat atctccggac gtctttccgt ggcatatcag aaatttggcg gctggtttga
     8281 cggtaacggc gatgccaccc tgcttgataa cacccagacc ggcctgcagc actccaatcg
     8341 gctggacatc atgggaaccg gtacgctgaa catcgatgaa tcccggcagc ttcaactgat
     8401 aacgcagtac tataaaagtc agggagacga caattacggg cttaatctcg ggaaaggctt
     8461 ttccgccatc agcgggagca gcacaccata cgtcagtaag gggctgaatt ctgaccgcat
     8521 tcccggcact gagcggcatt tgatcagcct gcagtactct gacagtgatt tcctgggaca
     8581 ggaactggtc ggtcaggttt actaccgcga tgagtcgttg cggtactacc cgttcccgac
     8641 ggtaaatgcg aataaacagg cgacagcttt ctcctcgtca cagcaggata ccgaccagta
     8701 cggcatgaaa ctgactctga acagccaact gatggacggc tggcaaatca cctgggggct
     8761 ggatgctgag catgagcgct ttacctccaa ccagatgttc ttcgatctgg ctcaggcaag
     8821 tgcttccgga gggctgaaca accataagat ttacaccacc gggcgctatc cgtcatatga
     8881 catcaccaat ctggcggcct tcctgcaatc cagctatgac attaatgata tttttaccgt
     8941 tagcggtggc gtacgctatc agtatactga gaacagggta gatgatttca tcgactacac
     9001 gcagcaacag aagattgctg ccgggaaggc gatatctgcc gacgccattc ctggtggttc
     9061 ggtagattac gataactttc tgttcaatgc tggtctgctg atgcacatca ccgaacgtca
     9121 gcaggcatgg ttcaattttt cccagggggt ggcattgccg gatccgggga aatattatgg
     9181 tcgcggcatc tatggtgcag cagtgaacgg ccatctttcc ctgacaaaga gcgtgaacgt
     9241 cagcgacagt aagctggaag gcgtgaaagt cgattcttat gaactgggct ggcgctttac
     9301 cggtgacaac ctgcggactc aaatcgcggc atattactcg ctttccaata agagcgtgga
     9361 aaggaataaa gatctgacca tcagtgtgaa ggacgacagg cgccgtattt acggcgtgga
     9421 aggtgcggtg gactacctga tcccggatac tgactggagt accggtgtga acttcaatgt
     9481 gctgaaaacc gagtcgaaag tgaacggtca atggcaaaaa tatgacgtga aggaatcaag
     9541 tccatcgaaa gcgacagctt acattaactg ggcgccggaa ccgtggagtc tgcgtgtaca
     9601 gagcaccact tctttcgacg taagcgatgc agagggtaac gatattaatg gttacactac
     9661 cgtcgatttt atcagtagtt ggcagcttcc ggtgggaaca ctcagcttca gcgttgagaa
     9721 cctcttcgac cgtgactata ccactgtctg gggacagcgt gcacctctgt actactgccc
     9781 gggttacggc cctgcttcac tgtacgacta caaaggccgg ggccgaacct ttggtctgaa
     9841 ctactcagtg ctgttctgac ggggatgctt ttgcaacaag ggtatgctgt aaacaacatg
     9901 gtccttggtg gccatgttgt ttacagccag tataatatac agccttaaca agatattccg
     9961 tctccggaat ggactgttca ccactgaata acattatttc tgttttatgg atctttgact
    10021 gaaatttatc gcatatcctc atttcattgt ccgtctgaaa actaacctta caagtaagcg
    10081 tcgtctcagc accgtctggc agatcctgaa attcctgaga ggatgatctg cgcccaatat
    10141 cctgagccgg aactttgctg caatgaaggc caacgaaaaa tgggggagta tgttatagag
    10201 tacttgctac agggtaaaaa gaggtacctg tcgctggttc tccgttcagt ttaaccggga
    10261 gattttctcc tgcgcctgtc ataaagtccg gtaatggaga ttgttaatac gatggtgcac
    10321 gatgcattgt cggtgcccgg cccggaggat gccccgtaac tacatacgga tcagggttgg
    10381 caatatcgaa tggcaggcta taggcaaagt taaagcccca tggagtagca caaaatatgc
    10441 cacacaaagg aaacggtctg aataacgcag tgatggagaa cttcttcggc acactgcaaa
    10501 atcgcatcgt ggtcgatcgc taattcgccg tgaatcactg aacgatgttc catgcgaaca
    10561 gatattcgat catatcgaaa tattaaacat tacccctccc cgcattgggc aaaaaaaaaa
    10621 aacaaaaaac gatcaattag tattgattca cataatctct catcattaat gagaacattg
    10681 tcacatattt acaatataga actgttactt ttagtaagct gttagtaacc aactgttact
    10741 aataatttta attcttaata ctttgttcat tcaataaatg agttgagag
//
LOCUS       DAZRUF010000145        10687 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZRUF010000145 DAZRUF010000000
VERSION     DAZRUF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.145"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHXOL_002913"
     CDS             complement(<1..784)
                     /locus_tag="ACHXOL_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR6809846.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHXOL_002914"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHXOL_002914"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR6809847.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGLGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHXOL_002915"
     CDS             1860..2747
                     /locus_tag="ACHXOL_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR6809848.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASVVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHXOL_002916"
     CDS             2780..3790
                     /locus_tag="ACHXOL_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR6809849.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDVVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHXOL_002917"
     CDS             3860..5293
                     /locus_tag="ACHXOL_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6809850.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHXOL_002918"
     CDS             5392..6741
                     /locus_tag="ACHXOL_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6809851.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHXOL_002919"
     CDS             6731..7618
                     /locus_tag="ACHXOL_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR6809852.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHXOL_002920"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHXOL_002920"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR6809853.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHXOL_002921"
     CDS             8352..9224
                     /locus_tag="ACHXOL_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR6809854.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHXOL_002922"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHXOL_002922"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR6809855.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHXOL_002923"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHXOL_002923"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR6809856.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2530 c         2855 g         2840 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcctagg aatggggatc cgcgtgaagc taattaacaa ctatatgagc attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgtcgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgttgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatt gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacagggg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tctcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggtc
//
LOCUS       DAZRUF010000146        10547 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZRUF010000146 DAZRUF010000000
VERSION     DAZRUF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.146"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(72..1100)
                     /gene="malI"
                     /locus_tag="ACHXOL_002924"
     CDS             complement(72..1100)
                     /gene="malI"
                     /locus_tag="ACHXOL_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR6809857.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1275..2867
                     /gene="malX"
                     /locus_tag="ACHXOL_002925"
     CDS             1275..2867
                     /gene="malX"
                     /locus_tag="ACHXOL_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR6809858.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2877..4049
                     /gene="malY"
                     /locus_tag="ACHXOL_002926"
     CDS             2877..4049
                     /gene="malY"
                     /locus_tag="ACHXOL_002926"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR6809859.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4153..5154
                     /gene="add"
                     /locus_tag="ACHXOL_002927"
     CDS             4153..5154
                     /gene="add"
                     /locus_tag="ACHXOL_002927"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR6809860.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5190..6230)
                     /locus_tag="ACHXOL_002928"
                     /pseudo
     CDS             complement(5190..6230)
                     /locus_tag="ACHXOL_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            6474..6599
                     /gene="blr"
                     /locus_tag="ACHXOL_002929"
     CDS             6474..6599
                     /gene="blr"
                     /locus_tag="ACHXOL_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR6809861.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6864..7079
                     /gene="ydgT"
                     /locus_tag="ACHXOL_002930"
     CDS             6864..7079
                     /gene="ydgT"
                     /locus_tag="ACHXOL_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR6809862.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7165..7605
                     /locus_tag="ACHXOL_002931"
     CDS             7165..7605
                     /locus_tag="ACHXOL_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR6809863.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7682..8263
                     /gene="rsxA"
                     /locus_tag="ACHXOL_002932"
     CDS             7682..8263
                     /gene="rsxA"
                     /locus_tag="ACHXOL_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR6809864.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8263..8841
                     /gene="rsxB"
                     /locus_tag="ACHXOL_002933"
     CDS             8263..8841
                     /gene="rsxB"
                     /locus_tag="ACHXOL_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR6809865.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8834..>10547
                     /gene="rsxC"
                     /locus_tag="ACHXOL_002934"
     CDS             8834..>10547
                     /gene="rsxC"
                     /locus_tag="ACHXOL_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR6809866.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKL"
BASE COUNT         2409 a         2729 c         2700 g         2709 t
ORIGIN      
        1 cctgtgtgcc acatttttta tcattaacct tgaggaaagg acactaccag gtatagcaga
       61 taaagagagg attatttcgc tgcaatgagc cgggcgggaa taataagatt gcgtgaatgc
      121 gtctcttcat gggtgatttt ttgcatcatg cgatccgcaa gtgtggtacc aagttcccgc
      181 gcaggcgtgc tggcccaggt aacgggtata tcatcaagtg tggttggtgt cgcatcggta
      241 aatgccgcca gcgaaacttg ttgctcaaag taacgatcga ctccgctttc cccgctttgc
      301 cgccctgctt tcagcaaacc aaaccatgcc cccatcgcaa tagtttcgtt atagcaaacc
      361 acagcactga tggtcgggtt atgacgtaaa agcgccgtga tagcttccgc ggcttgcttc
      421 tggctggatg tgcactccag cacccaatcg ctgtgaaacg gcaggccaaa ttttagtaga
      481 gttgcacaat agcctcccac ccgttctgcc cgggttaatg aggaactttg ccctcccagc
      541 caggcgatcc gctgatgccc attgcgaatg agatgctccg tcaacaactg tgcagcctgc
      601 atgttgtccg ggcgaaccgt atcaacatca tcaagataac tggcacggga agcgaaaatc
      661 accgggatag ctttttcttc tgccatccgt cgcaggtcat cgctgcttcc tgcagccccg
      721 gcaattacca caccatcgac accctgattc agtaacagtg aaaaccgctg tgccagctgc
      781 tcgccgtctt taccgccgtg aagcaaaaaa accatccgtc cctgcgcttc cagagcttcc
      841 gtcaatccgg ccgtcaattc ggcgtaaaac ggcgcagata aatcacggac gatcaaacca
      901 atgacgccgc tttgcccgcc gcgcagcgcc gacgcctggc gattgcgcac aaatcccagc
      961 tcttcaatgg cggcgttaac gcgttctcct gtggcggtag agattcgccc tttgccacta
     1021 agcaccagcg aaacggtact taccgacacg cccgcagcca gcgcaacatc atgaatggtt
     1081 atttttttgg cggtagccat gcagatgacc tactccctga ttatgtgaca gataaaacgt
     1141 tttacctttt atttatctta tacccgctat tatcgttgcg taatgtgatt tatgcttcac
     1201 taaaatttga taaaacgttt tatcttctcg cgcaatttac tgaatccaga ttgttctcta
     1261 cgaggagtcg ttttatgacg gcgaaaacag caccgaaagt cacgctgtgg gagttcttcc
     1321 agcagttagg caaaaccttc atgttacccg tggcattatt gtcgttctgc ggcattatgc
     1381 tcggcattgg tagttctctt agtagccacg atgtcatcac cctgatcccg gtcctgggca
     1441 accccgtgtt gcaggctatc tttacctgga tgagtaagat tggctcgttt gcttttagtt
     1501 tcctgccagt catgttctgt atcgccatcc cgctgggtct ggcacgcgaa aacaaaggcg
     1561 tagcggcatt cgctggcttc gtcggttatg cggtaatgaa cctcgcggta aacttctggt
     1621 tgaccaataa aggcattctg ccaaccacgg acgccgcggt tctgaaagcc aataacatcc
     1681 agagcattct tgggatccag tcgatcgaca ccgggatcct cggtgcggtg atcgccggta
     1741 ttatcgtctg gatgctgcat gagcgattcc ataatatccg cctgccggat gcgcttgcat
     1801 tcttcggcgg tacgcgcttc gtaccaatca tctcctcgct ggtgatgggc cttgtcggcc
     1861 tggtgattcc attagtctgg ccgattttcg cgatgggtat tagcggcttg ggccatatga
     1921 taaacagcgc gggtgatttc ggaccgatgc tgtttggtac cggtgaacgt ctgctgttgc
     1981 cgtttggtct gcatcacatt ctggtggcat taattcgctt taccgacgca ggcggcacgc
     2041 aggaagtctg cggtcaaacc gtcagcggcg cattgaccat cttccaggcg caattgagtt
     2101 gcccgaccac tcacggtttt tctgaaagcg ccacgcgttt cctttcgcaa ggcaaaatgc
     2161 ctgcgtttct cggcggtctg ccaggtgcag cgttagcgat gtatcactgc gcgcgcccgg
     2221 aaaatcgcca taaaattaaa gggctgctga tttctggcct gatcgcctgt gtcgttggcg
     2281 gcacgaccga accgctggaa ttcctgttcc tgttcgtggc gccagttctg tatgtcatcc
     2341 acgcgctgtt aaccggcctc ggcttcactg tcatgtctgt gctcggcgtc accatcggta
     2401 ataccgacgg caatatcatc gacttcgtgg tgttcggtat tttgcatggt ctgtcaacca
     2461 agtggtacat ggtgccagtg gtggcggcaa tctggtttgt cgtttactac gtcatcttcc
     2521 gtttcgctat cacccgcttc aatctgaaaa ccccggggcg cgatagcgaa gtggccagtt
     2581 caatcgaaaa agccgttgcc ggtgcgccgg gtaaatcagg ttacaacgtt ccggcaatcc
     2641 tcgaagcctt aggcggtgcc gacaatattg ttagcctcga taactgcatt acccgtctgc
     2701 gtttgtctgt gaaagatatg tcgcttgtta atgtgcaggc actgaaggac aatcgggcaa
     2761 ttggcgtagt acaacttaat caacataacc tgcaggttgt tatcgggcca caagttcagt
     2821 cagtaaaaga tgaaatggcc ggtctgatgc atactgtcca ggcataagga taagatatgt
     2881 tcgatttttc aaaggtcgtg gatcgtcatg gcacatggtg tacacagtgg gattatgtcg
     2941 ctgaccgttt cggcactgct gacctgttac cgttcacgat ttcagacatg gattttgcca
     3001 ctgccccctg cattatcgag gcgctgaatc agcgcctgat gcacggcgta tttggctaca
     3061 gccgctggaa aaacgatgag tttctcgcgg ctattgccca ctggttttcc acccagcatt
     3121 acaccgcaat tgatacgcag acggtggtgt atggcccttc tgtcatctat atggtttcag
     3181 aactgattcg tcagtggtct gaaacaggtg aaggcgtggt gatccacacg cccgcctatg
     3241 acgcatttta caaggccatt gaaggtaacc agcgcacagt aatgcccgtt gctttagaga
     3301 agcaggctga cggctggttt tgcgatatgg gcaagctgga agccgttttg gcgaaaccag
     3361 aatgtaaaat tatgctcctg tgtagcccac agaatcctac cgggaaagtg tggacgtgcg
     3421 atgagctgga gatcatggct gacctgtgcg agcgtcatga tgtgcgggtt atttccgatg
     3481 aaatccatat ggatatggtc tggggcgagc agccgcatat tccctggagt aatgtggcgc
     3541 ggggagactg ggcgttgctg acgtcgggat cgaaaagttt caatattccc gcgctgaccg
     3601 gtgcttacgg gattatagaa aatagcagca gccgtgatgc ctatttatcg gcactgaaag
     3661 gacgtgatgg gctttcttcc ccttcggtac tggcgttaac tgcccatatc gccgcctatc
     3721 agcaaggtgc accgtggctg gatgccttac gcgtctatct gaaagataac ctgacgtata
     3781 tctcagataa aatgaacgcc gcgtttcctg aactcaactg gcagatcccg caatccactt
     3841 atctggcctg gcttgatcta cgtccgttga atattgacga caacgcgttg caaaaagcgc
     3901 ttatcgagca agaaaaagtc gcgatcatgc cggggtatac ctatggtgaa gaaggtcgtg
     3961 gttttgtccg tctcaatgcc ggctgcccac gttcgaaact ggaaaaaggt gtggctggat
     4021 taataaacgc catccgcgct gttcgctaac cccaattgcg caacgtaaaa aatcgttgcg
     4081 caatcgtgga tttttaccct gctttgtttt tataatggtg cgcactttta tatccagaaa
     4141 aagagtgcga ccatgattga taccaccctg ccattaactg atatccatcg ccaccttgat
     4201 ggcaacattc gtccccagac cattcttgaa cttggccgcc agtataatat ctcgcttcct
     4261 gcacaatccc tggaaacact gattccccac gttcaggtca ttgccaacga acccgatctg
     4321 gtgagctttc tgaccaaact tgactggggc gttaaagttc tcgcctctct tgatgcctgc
     4381 cgccgcgtgg catttgaaaa cattgaagat gcagcccgta acggcctgca ctatgtcgag
     4441 ctgcgttttt caccaggcta catggcaatg gcacatcagc tgcctgtagc gggtgttgtc
     4501 gaagcggtga tcgatggcgt acgtgaaggt tgccgcacct ttggtgtgca ggcgaagctt
     4561 atcggcatta tgagccgaac cttcggcgaa gccgcctgtc agcaagagct ggaggccttt
     4621 ttagcccacc gtgaccagat taccgcactt gatttagctg gtgatgaact tggattcccg
     4681 ggaagtctgt tcctttctca cttcaaccgc gcgcgtgatg cgggctggca tattaccgtc
     4741 catgcaggcg aagctgccgg gccggaaagc atctggcagg cgattcgtga actgggtgcg
     4801 gagcgtattg gacatggcgt aaaagccatt gaagatcggg cgctgatgga ttttctcgcc
     4861 gagcaacaaa ttggtattga atcctgtctg acctccaata ttcagaccag caccgtagca
     4921 gagctggctg cacatccgct gaaaacgttc cttgagcatg gcattcgtgc cagcattaac
     4981 actgacgatc ccggcgtaca gggagtggat atcattcacg aatataccgt tgccgcgcca
     5041 gctgctgggt tatcccgtga gcaaatccgc caggcacaga ttaatggtct ggaaatggct
     5101 ttcctcagcg cagaggaaaa acgcgcactg cgagaaaaag tcgccgcgaa gtaacaaaaa
     5161 tggatggtgc aatttgcacc atccattttt cacgcaaggc acaacgtcgc gcgatgtttg
     5221 gcggattcga tgcccagctc aatcaactcc attacctgga ttgcctagct tgccggaacc
     5281 ggattttcac catcgccatt taacgcatca cgaatcgccg catagtaagc cggataattc
     5341 ccaggcaccg tcaacagcgt ttcttcgaca cgttcctcac cttccacgcg ggtaagtacg
     5401 ccatcacgca tatcgtagcc ccagtcttcc tgcggcagac gctcgccatt tttcagacgt
     5461 tcttcctgtg gatcgaggcc atatttcaca taactgcctc gggatccatg cacgatatac
     5521 cgtgctgact cagcggctgc cagcatggta ccgtgtaaaa tgactcgccg ctgtggatag
     5581 gacaagatgg cgtggaaata atcggtcgac tgcgctccgg gccgtaactg tgccaaatct
     5641 accgtcatgc tgaccggtaa accaaatagc gtaatggcct gatcaagaag atgcggtgct
     5701 aaatcgtacc agataccgct gcctgggccg ccctgttcac gccaacgatc gcgcacctgc
     5761 ggacggaagc ggtcaaaatg agactcaaag taagcaactt cacccagcac gccttccgcg
     5821 agcaaacctt ttagcgtcaa gaaatcgcta tcccaacgac ggttatggaa tacagacagc
     5881 acacgcccca ggctttttgc cagcgcatcc agctctcgcg cttgtgacag tgtcacggca
     5941 aagggtttat caacgaccac atgtttaccc gcctcaagcg ccgctttggc taacgggaaa
     6001 tgggtatcgt tgggtgtagg aatgacaatc aggtctatgt tgggatcgtt aaacagatgc
     6061 ttcggctcag agacaaccgt aaccgttggc cagtcggctt ttacttttgt ttcatcactg
     6121 ctggagatta ccgccagttc cagcccgggc gtgcccgcaa tcaggggcgc atggaaggtt
     6181 ttgctcgcat aaccataccc aatcaaccca acacggatgt tgtcgctcat gtcattgcct
     6241 ctcattgaag tatgatggct atttgacact atcctttacc cacgctcaac agtttaataa
     6301 cctgccagca ataagggatg ttgtttaact taagtcaaaa aaaatagcga attttccaac
     6361 gacaaaagct aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa
     6421 gtaacattta ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc
     6481 gtcttattga attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg
     6541 cgagtcacat tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag
     6601 ctctgcactt gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat
     6661 taaaccagga atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg
     6721 cgttactctt ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt
     6781 attgtaataa cacaagcgtt cacttttggt gaaattttat catcactcta cccctttggt
     6841 attaattatt ttatggacct tttatgactg ttcaggacta cttattaaaa tttcgcaaaa
     6901 tcagttcact cgaaagtctg gaaaaactct acgaccatct taattacacc ctgacggacg
     6961 atcaggaact gatcaatatg tatcgtgctg ccgatcaccg tcgcgcagag ctggtttctg
     7021 gcgggcgttt gtttgacctc ggccaggtac cgaagtccgt ctggcactat gtccaataaa
     7081 gaaagtagcg atcggttata aaagccttat aataacgccc ctaaaattag atttgcctgt
     7141 ttgcgtcgct attctggaga acctatgacc accacaacgc cacaacgtat tggaggctgg
     7201 ttgcttggcc cgttggcttg gcttttagtc gctctgttga gtacgacgct ggcgttgttg
     7261 ctgtataccg ctgcgttatc ttctccgcaa acatttcaaa cgctcggcgg acaagcactg
     7321 acgacacaaa tcttatgggg cgtttctttt attaccgcta tcgccatgtg gtattacaca
     7381 ctgtggttga ccatcgcttt ctttaaacgt cggcgttgtg ttcctaaaca ctatattatc
     7441 tggctgctta tttccgtatt gctggcagta aaagcattcg ctttttcacc cgtggaagat
     7501 ggcatagccg ttcgccagtt gctgtttact ttgttggcaa ccgcattgat cgtcccctat
     7561 ttcaaacgct cgtcgcgggt aaaagcgacg tttgtgaatc cgtaataacc ttacagttaa
     7621 cctgttgtcg cctgctctgg attaacggat aataggcggc ttttttattt caggccgaaa
     7681 aatgactgac tacctgttac tgtttgtcgg aactgtactg gtcaataact ttgtactggt
     7741 caagtttctc ggtctctgtc cgtttatggg ggtttccaaa aaactggaaa ccgcgatggg
     7801 catggggctg gcaacaacgt ttgtgatgac gctggcgtct atttgcgcct ggcttatcga
     7861 tacgtggatt ttgatcccac ttaatctgat ttacctgcgc accctggcat ttattctggt
     7921 gattgctgtg gtcgtgcagt tcaccgagat ggtggtgcgc aaaaccagcc cggtgcttta
     7981 ccgtctgctg gggatttttt tgccgcttat caccaccaac tgtgcagtgc tcggcgtggc
     8041 gttgctgaat atcaatctcg ggcacaattt cttgcagtcg gcgctgtacg gtttttccgc
     8101 cgctgtcggt ttctcgctgg tgatggtgct cttcgccgcc atccgcgaac gccttgctgt
     8161 ggctgatgtc ccggcacctt ttcgcggtaa tgccattgcg ttaattaccg caggtcttat
     8221 gtctctggcc tttatgggct ttaatggttt ggtgaagttg taatgaatgc tatctggatt
     8281 gccgttgccg ccgtgagcct gctgggcctg gcgtttggcg ccattctggg ttatgcctcc
     8341 cgccgttttg cggtggaaga cgatccggtc gttgagaaaa ttgacgaaat cttaccgcag
     8401 agccagtgtg gtcagtgcgg ttatcccggc tgtcgcccct acgcggaagc catcagctgt
     8461 aacggtgaaa aaatcaaccg ttgcgcccca ggtggcgaag ctgtgatgct aaaaattgcc
     8521 gagttgctta atgtcgagcc gcagccgctg gatggcgaag cgcaagagct aacgcctgcg
     8581 cggatggtgg cggttattga tgaaaataac tgtattggct gcactaaatg tattcaggct
     8641 tgtccggtag acgccatcgt tggcgctacc cgtgccatgc atacggtaat gagtgatctc
     8701 tgtacgggct gcaatttatg tgttgatccg tgcccgacgc actgcatctc gttgcaaccg
     8761 gtcgcagaaa cacctgactc ctggaaatgg gatctgaaca ccattcccgt gcgtatcatt
     8821 cccgtggaac accatgctta agttattctc tgcattcaga aaaaataaaa tctgggattt
     8881 caacggcggc atccatccac cggagatgaa aacccagtcc aacggtacac ccctgcgcca
     8941 ggtaccactg gcgcagcgtt ttgttattcc actgaaacag catattggcg ctgaaggtga
     9001 gttgtgcgtt agcgtcggcg ataaagtatt gcgcggccag ccgcttaccc gtggtcgcgg
     9061 caaaatgctg cctgttcacg cgcccacctc gggtaccgtt acggctattg cgccccactc
     9121 tacggctcat ccttcagctt tagctgaatt aagcgtgatt attgatgccg atggtgaaga
     9181 ctgctggatc ccgcgcgacg gctgggccga ttatcgcact cgcagtcgcg aagagttaat
     9241 cgagcgcata catcagtttg gtgttgccgg gctgggcggt gcaggattcc cgacaggcgt
     9301 taaattgcag ggtggcggag ataagattga aacgttgatt atcaacgcgg ctgagtgcga
     9361 gccgtacatt accgccgatg accgtttgat gcaggattgc gcggctcagg tcgtagaggg
     9421 tattcgcatt cttgcgcata ttctgcagcc acgcgaaatt cttatcggca ttgaagataa
     9481 caaaccgcag gcgatttcca tgctgcgcgc ggtgctggcg gactctaacg atatttctct
     9541 gcgggtgatt ccaaccaaat atccttctgg cggtgctaaa caattaacct acattctgac
     9601 cgggaagcag gttccacatg gcgggcgttc atccgatatc ggcgtattaa tgcaaaacgt
     9661 cggcactgct tatgcagtga aacgtgccgt tattgatggc gagccgatta ccgagcgtgt
     9721 tgtaaccctg actggcgaag caatcgctcg cccgggcaac gtctgggcac ggctggggac
     9781 gccagtgcgt catttattga atgatgccgg attctgcccc tctgccgatc aaatggtgat
     9841 tatgggtggc ccgctaatgg gctttacctt gccatggctg gatgtcccgg tcgtaaagat
     9901 taccaactgt ctgttggctc cctctgccaa tgaacttggc gaaccacagg aagaacaaag
     9961 ctgcatccgg tgtagcgcct gtgctgacgc ctgccctgcg gatcttttgc cgcaacagtt
    10021 gtactggttc agcaaaggtc agcaacacga taaagctacc acgcataaca ttgctgattg
    10081 cattgaatgt ggggcttgcg cgtgggtttg cccgagcaat attcccctgg tgcaatattt
    10141 ccgtcaggaa aaagctgaaa ttgcggctat tcgtcaggaa gaaaagcgcg ccgcagaagc
    10201 caaagcgcgt ttcgaagcgc gccaggctcg tctggagcgc gaaaaagcgg ctcgccttga
    10261 acgacataag agcgcagccg ttcaacctgc agccaaagat aaagatgcga ttgctgccgc
    10321 tctggcgcgg gtgaaagaga aacaggccca ggctacacag cctattgtga ttaaagcggg
    10381 cgaacgcccg gataacagtg caattattgc agcacgggaa gcccgtaaag cgcaagccag
    10441 agcgaaacag gcagaactgc agcaaactaa cgacgcagca accgttgctg atccacgtaa
    10501 aactgccgtt gaagcagcta tcgcccgcgc caaagcgcgc aagctgg
//
LOCUS       DAZRUF010000147        10423 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZRUF010000147 DAZRUF010000000
VERSION     DAZRUF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10423
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.147"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            46..621
                     /gene="ssuE"
                     /locus_tag="ACHXOL_002935"
     CDS             46..621
                     /gene="ssuE"
                     /locus_tag="ACHXOL_002935"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR6809867.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            614..1573
                     /gene="ssuA"
                     /locus_tag="ACHXOL_002936"
     CDS             614..1573
                     /gene="ssuA"
                     /locus_tag="ACHXOL_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR6809868.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1570..2717
                     /gene="ssuD"
                     /locus_tag="ACHXOL_002937"
                     /pseudo
     CDS             1570..2717
                     /gene="ssuD"
                     /locus_tag="ACHXOL_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            2729..3520
                     /gene="ssuC"
                     /locus_tag="ACHXOL_002938"
     CDS             2729..3520
                     /gene="ssuC"
                     /locus_tag="ACHXOL_002938"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR6809869.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3517..4284
                     /gene="ssuB"
                     /locus_tag="ACHXOL_002939"
     CDS             3517..4284
                     /gene="ssuB"
                     /locus_tag="ACHXOL_002939"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR6809870.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4491..7103)
                     /gene="pepN"
                     /locus_tag="ACHXOL_002940"
     CDS             complement(4491..7103)
                     /gene="pepN"
                     /locus_tag="ACHXOL_002940"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR6809871.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7369..8571
                     /gene="pncB"
                     /locus_tag="ACHXOL_002941"
     CDS             7369..8571
                     /gene="pncB"
                     /locus_tag="ACHXOL_002941"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR6809872.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8741..10141
                     /gene="asnS"
                     /locus_tag="ACHXOL_002942"
     CDS             8741..10141
                     /gene="asnS"
                     /locus_tag="ACHXOL_002942"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR6809873.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2347 a         2685 c         2821 g         2570 t
ORIGIN      
        1 tatcaatgtt aacaaaaaaa gaacaattgg ttataaggag agagtatgcg tgtcatcacc
       61 ctggcgggta gtcctcgctt tccttctcgc tccagctcct tgctggaata tgcgcgggaa
      121 aaactaaatg gcctggatgt agaggtttat cactggaatc tgcaaaactt cgccccggaa
      181 gatctgcttt acgctcgttt cgatagtccg gcactcaaga ccttcaccga acagctgcaa
      241 caggctgatg gactgatagt cgctacacct gtgtataaag ccgcctattc cggcgcgctg
      301 aaaaccctgc tcgacctgct gccagagcgc gctttgcaag gcaaagtggt gctaccgctg
      361 gcgacgggcg gtacggtggc tcatctgctg gcggtcgatt atgcccttaa gccagtttta
      421 agtgcgatga aagcccagga gctcctgcac ggcgtgtttg ccgatgactc acaagttatt
      481 gattaccatc acaaacccca gttcacgcca aatctgcaaa cccgtcttga taccgcgcta
      541 gaaactttct ggcaggcatt gcaccgccgc gatgttcagg ttcctgacct tctgtctctg
      601 cgaggtaatg tccatgcgta acatcattaa actggcgctg gcgggattgc ttagcgtctc
      661 tacttttgcg gttgctgcag aatcctcgcc tgaagcgtta cgtataggct atcagaaagg
      721 cagtattggt atggtattgg caaaaagcca ccagttactg gaaaaacgct atccgcaatc
      781 aaaaatctcc tgggtggagt tccccgcggg tccgcaaatg ttggaagcgt taaacgttgg
      841 cagtattgat ctcggcagta ccggggatat tccgccaatc tttgcccagg ctgccggggc
      901 tgatttgata tatgtgggcg tcgagccacc gaagcccaaa gccgaagtga ttctggtggc
      961 agaaaacagc ccgatcaaaa ccgtagccga tcttaaaggt cacaaagttg cctttcagaa
     1021 aggttccagt tcacacaacc ttttactgcg tgcactgcgt caggccggac ttaagtttac
     1081 tgacatccag cccacttatc tgacgccagc tgatgcccgc gccgcgttcc agcaaggtaa
     1141 cgttgacgcc tgggctatct gggatcccta ctactccgct gcgttattac agggcggcgt
     1201 gcgggtgctg aaagacggca ccgatctcaa tcaaactgga tcgttttatc tggcagctcg
     1261 cccctatgca gaaaaaaacg gcgcttttat tcagggcgta ctggcaacct ttagtgaggc
     1321 cgatgcgtta acccgcagcc agcgcgagca aagcatcgct ttactggcaa aaacgatggg
     1381 cttaccggca ccggtgattg cctcgtatct ggatcatcgt cctcccacca ccatcaaacc
     1441 ggtgagcgct gaagttgccg ccttacagca gcaaacggca gatctgtttt atgaaaaccg
     1501 tctggtgtcg aaaaaagttg atattcgcca gcgcatctgg cagcccactc acctggaagg
     1561 aaaacaatta tgagtctgaa tatgttctgg tttttaccga cccatggtga cgggcattat
     1621 cttggaacgg aagaaggttc acgcccggtt gatcacggct atctgcaaca aattgcgcaa
     1681 gcggcggatc gtctgggcta taccggtgtg ctgatcccaa cggggcgatc gtgtgaagat
     1741 gcgtggctgg tggcggcgtc gatgatcccg gtgacgcagc ggctgaagtt tcttgtcgcc
     1801 ctgcgtccca gcgtaacctc acctaccgtt gccgctcgcc aggccgccac gcttgaccgt
     1861 ctctcaaatg gtcgtgcgtt gtttaatctg gtcacaggca gcgatccaca agagttggca
     1921 ggcgacggcg tgttccttga tcatagcgag cgctacgaag cctcggcgga atttacccag
     1981 gtctggcggg gcgcttattg ctgggggaaa gcgtcgattt caacggtaaa catattcatg
     2041 tgcggggagc aaaactgctc ttcccgccga ttcaacagcc gtatcctccg ctttactttg
     2101 gcggatcgtc agatgtcgcc caggagctgg cggcagaaca agttgatctc tacctcacct
     2161 ggggcgaacc gccagagctg gtaaaagaga agatcgaaca agtgcgggcg aaagctgccg
     2221 cgcatggacg caaaattcgt ttcggtattc gtctgcatgt gattgttcgt gaaactaacg
     2281 acgaagcgtg gcaggccgcc gagcggttaa tctcgcatct tgatgatgaa actatcgcca
     2341 aagcacaggc cgcattcgcc cggacggatt ccgtaggaca acagcgaatg gcggcgctac
     2401 ataacggcaa gcgcgacaat ctggagatca gccccaattt atgggcgggc gttggcttag
     2461 tgcgcggcgg tgccggaacg gcgctggtgg gcgatggtcc tacggtcgcc gcgcgaatca
     2521 acgaatacgc cgcgcttggc atcgacagct ttgtgctttc gggctacccg catctggaag
     2581 aagcgtatcg ggttggcgag ttgctgttcc cacatctgga tgtcgccatt ccggaaattc
     2641 cccagccgca gccgctgaat ccgcaaggcg aagcggtggc gaatgatttt atcccccgta
     2701 aagtcgcgca aagctaagga acacgatgat ggcaacgcca gtgaagaagt ggttattgcg
     2761 cgttgccccc tggtttttac cggtgggcat cgtggcggtg tggcaactgg cctcctcggt
     2821 tggctggctg tcgacgcgta ttttgccttc accggaagga gtggtgacgg cgttctggac
     2881 gctctccgcc agcggcgaat tgtggcagca tctggcgatc agttcctggc gggcgctgat
     2941 tggcttttca attggcggat cgctgggctt gattctggga ctgattagcg ggctgtcacg
     3001 ctggggagaa cggctgctgg atacctcaat tcagatgttg cgcaacgtac cgcatctggc
     3061 gctgatcccg ctggtgattt tgtggtttgg cattgatgaa tccgcaaaaa tctttctggt
     3121 ggcgctcggc acgctattcc ccatttatat caacacctgg catggcatcc gtaatatcga
     3181 tcgcggtctg gtggaaatgg cgcgtagcta tggattatcc ggcataccgc tgtttatcca
     3241 tgtgatcctg cctggtgccc tgccctcaat tatggtcggc gtgcgttttg cgttgggcct
     3301 gatgtggctg acgctgattg ttgccgaaac catttctgcc aattcaggca ttggttatct
     3361 ggcgatgaat gcgcgggagt ttttgcaaac ggacgtggtg gtagtcgcta ttattcttta
     3421 cgccctgctc ggcaaacttg ctgacgtcag cgcgcagctg ttagagcgcc tctggttgcg
     3481 ctggaacccg gcttatcatt tgaaggaggc cacggtatga atactgctcg tctgaaccag
     3541 ggcacaccat tgttgctcaa tgcagtaagc aaacattacg cggaaaatat cgtcctgaac
     3601 caactggatt tacatattcc ggcaggtcag tttgtggcgg tggtgggccg cagcggtggt
     3661 ggcaaaagta ccctgctgcg cctgctggca ggtctggaaa cgccaaccgc aggcgatgtg
     3721 ttagcgggca ccacaccgct ggctgagatt caggaagaca cgcgaatgat gtttcaggat
     3781 gcgcgtctgc tgccgtggaa atcggtgatt gataacgttg ggttaggcct taaaggccag
     3841 tggcgcgatg ccgcgcgtcg ggcgttggct gcagtaggac tggagaatcg cgccggggaa
     3901 tggcctgccg cactttctgg cgggcagaaa cagcgtgtgg cgctggcaag agcgttgatt
     3961 catcgaccgg gtttattgtt gcttgatgaa ccgctcgggg cactggacgc cttaacgcga
     4021 ctcgagatgc aggatttgat tgtgtcactt tggcagcagc acggctttac cgtactgttg
     4081 gtgacgcatg atgtgagcga agcggtagca atggctgacc gggtgttgtt aattgaagag
     4141 ggaaaaattg gtctggattt gacagtggat atcccccgtc cgcgtcgatt agggtcggtg
     4201 cgattagcgg agctggaagc ggaagtattg caacgggtaa tgcggcgtgg gcattctgaa
     4261 caactgattc ggcgtcatgg ttaatatgcc ggatgtggcg cacaaaacca gcattttcaa
     4321 cggacttgta ggcctgataa gacacgtcaa gcatcgcatc aggcagttgg gcacggctgc
     4381 cggatgcggc gtgaacgcct tatccagcct acaaaaccag caatttcaac ggagttgtag
     4441 gcctgataag acgcgtcaag cgtcgcatca ggcattttgg ctcaaaagta tcaagccagt
     4501 gctttggtga tcttctcgta cagatcgcca gagagatttt ccagcccttt caactgttcc
     4561 agcgccgcgc gcattttctc ctgacgtttg gcatcataac gtttcaggcg aatcagcggt
     4621 tcaatcagac gtgaagctac ctgcgggtta cggctgttga ggtcggtaag catttccacc
     4681 aggaactggt aaccgctgcc atcttcggca tggaacgctg ccgggttgct gcccgcaaac
     4741 gcaccaatca acgaacgaat acggttcggg ttgctcatgg taaatgagcg atgctgcaac
     4801 agaccacgca ccgtctccag cacattcgcc gccgggctgg tggcttgcag gataaaccat
     4861 ttatccatca ccagaccgtc ctgatgccac ttatcgtcgt actcctgcat cagcgcgtca
     4921 cggcaaggca gctgtgcagc aaccgccgca gaaagcgctg ccagcgcatc ggtcatattg
     4981 tttgcttcat ggtattgctt gctcaccagc acgtcagcca gatgcgtttc accaaaagca
     5041 aggaagcgca ggcaggcatt tcgcagagtg cgttttgcaa tatcttcatg ctcaacacgg
     5101 tactcgctct ggtaattcgc gttgtaaata gccagcagct catccgccag ttcagtcgcc
     5161 agagtacgag tgagtgcttc gcgtacttcg gcaatagcaa tcgggtcgat gatatcgaac
     5221 agttcagcca tttcattgac agaaggcagc gtcaggattt ctgccgccag cgccggatca
     5281 atcttctcat cgagcagcac cgcgcggaaa gcgtcagcca catgtaccgg cagagacagc
     5341 ggctgccctt gctgatgacg ggcgacgttc agcttgatgt aggttgccag caaactttgc
     5401 gccgcatccc aacgggagaa atcattacgc gcatgacgca tcaggaaggt cagttgctga
     5461 tcactccact tatattccag tttcactggc gcagagaatt cgcacagcag cgcaggcacc
     5521 ggctggaagt agacattatc aaaaacaaag gtctgttccg cctgggtgac gttcagcacg
     5581 gaattcaccg gatgaccgcc tttctgcaac gggatcactt tgccttcgtt gtcatacaat
     5641 tcgatggcaa acggaatatg cagcggctgt ttttctgcct gatccggcgt ggctggcgtg
     5701 cgctggctga tggtcagggt gtactgctca gtttccggat tgtagtcgtc tttgacggtc
     5761 acaatcggcg taccggactg gctgtaccaa cggcggaaat gggagagatc aacattcgac
     5821 gcatcttcca tcgcctgcac aaagtcgtca caggtcgctg cgctaccatc atgacgctcg
     5881 aaatagagtt gcatcccttt ctggaagttt tcttcgccca gcagggtgtg gatcatacgg
     5941 atcacttccg cgcccttctc gtaaacggtc agggtgtaga agttgttcat ctcaatgacc
     6001 atgtccgggc ggattgggtg cgccatcggg ctggcgtctt ccgcaaactg caatccgcgc
     6061 atggtgcgca cattattgat gcggttaact gcgcgggaac caaggtcaga gctgaattcc
     6121 tgatcgcgga agacggttaa accttctttc aggctgagct ggaaccagtc gcggcaggtc
     6181 actcggttac cggtccagtt atggaaatat tcatggccga taacgcgttc aatatcgagg
     6241 taatctttgt cggtggcggt gtcggtgcgg gccagcacat atttggagtt aaagatattc
     6301 agacccttat tctccattgc gcccatattg aagaaatcca ccgcgacgat catatagatg
     6361 tcgaggtcat actccaggcc gaagcgttct tcatcccatt tcattgagtt tttcagcgag
     6421 gtcatcgccc acggcgcgcg atcaaggttg ccgcgatcga cgtacagctc cagcgccact
     6481 tcgcgaccag aacgcgtggt aaaggtgtcg cgcagtacat caaagtcgcc tgccaccagc
     6541 gcaaacaggt agcacggttt cgggaacggg tcctgccact gtacccaatg gcgtccgttt
     6601 tccagttcac cttgcgcaac gcggttaccg ttggaaagca ggaagggata tttggtttta
     6661 tcggcaataa ttttggtggt aaaacgcgcc agcacgtccg ggcggtcgag ataatacgta
     6721 atatggcgga aaccttcggc ttcacactgg gtgcaaagcg catcgcctga ctgataaagc
     6781 ccttccagcg cggtattcgc cgccgggctt atttcattaa cgatcttaag cgtaaaatgc
     6841 tccggcaaat tactgatgac cagtgcgccc tcttcttctt tccaggcggt ccacggctca
     6901 tcattaatat gaacagaaac cagtttgagg tcttcgccgt tgagacgaag tggagcatct
     6961 gatgcaccat gacggacagc ctggctgacc gcggtaacga ccgtcttttg cgcgtcgagg
     7021 tcaaaggtca agtcaatatc agtaatctgg taatccggcg cacgataatc gtgacggtat
     7081 ttggcttgtg gctgttgagt cataaataac ctttagcatc ttttatagag tctggtgttc
     7141 agtctattcc tgttgcgtaa atcgcgctat gcagaatctt catcttttca ggtacaaacg
     7201 cctttattgc tacattttta taacatacac cgcgtaatgc catcgaccag aaaggtggca
     7261 tatggtgtga tcggggttca ataaattgcg aaacaaggta tactccagca gttcctgaag
     7321 atgtttattg tactaaacgc tcctgtacga ggacgctact gcgcacctat gacacaattc
     7381 gcttctcctg ttctgcactc gttgctggat acagatgctt ataagttgca tatgcagcaa
     7441 gccgtgtttc atcactatta cgatgtgcat gtcgcggcgg agtttcgttg ccgaggtgac
     7501 gatctgctgg gtatttatgc cgatgctatt cgtgaacaga ttcaggcgat gcagcacctg
     7561 cgcctgcagg atgatgaata tcagtggctc tctgccctgc ctttctttaa ggccgactat
     7621 cttaactggt tacgcgagtt ccgctttaac ccggaacaag tcaccgtgtc caacgataat
     7681 ggcaagctgg atattcgctt aagcggtccg tggcgtgaag tcatcctctg ggaagttcct
     7741 ttgctggcgg ttatcagtga aatggtacat cgctatcgct caccgcaggc cgacgttgcg
     7801 caagccctcg acacgctgga aagcaaatta gtcgacttct cggcgttaac cgccggtctt
     7861 gatatgtcgc gcttccatct gatggatttt ggcacccgtc gccgtttttc tcgcgaagta
     7921 caagaaacca tcgttaagcg tctgcaacag gaatcctggt tcgtgggcac cagcaactac
     7981 gatctggcgc gtcggctttc cctcacgccg atgggaacac aggcacacga atggttccag
     8041 gcacatcagc aaatcagccc ggatctagcc aacagccagc gagctgcact tgctgcctgg
     8101 ctggaagagt atcccgacca acttggcatt gcattaaccg actgcatcac tatggatgct
     8161 ttcctgcgtg atttcggtgt cgagttcgct agtcggtatc agggcctgcg tcatgactct
     8221 ggcgacccgg ttgaatgggg tgaaaaagcc attgcacatt atgaaaagct gggaattgat
     8281 ccacagagta aaacgctggt tttctctgac aatctggatt tacgcaaagc ggttgagcta
     8341 taccgccact tctcttcccg cgtgcaatta agttttggta ttgggactcg cctgacctgc
     8401 gatatccccc aggtaaaacc cctgaatatt gtgattaagt tggtagagtg taacggtaaa
     8461 ccggtggcga aactttctga cagccctggc aaaactatct gccatgataa agcgtttgtt
     8521 cgggcgctgc gcaaagcgtt cgaccttccg catattaaaa aagccagtta atatcatcag
     8581 ggagctaatc ggctcccttt ttttaccttt aattccgaaa tctttcgctg catttgcgaa
     8641 ttctgcttgt ctgattgcag atgccaggta acataggtat cccccccatt tacgggtgga
     8701 caagtgttta ttttttccga ctattaacag agagaatatt atgagcgttg tgcctgtagc
     8761 cgacgtactc cagggccgcg tagccgttga cagcgaagtc accgtacgcg gatgggtacg
     8821 tacccgccga gattcaaaag ctggcatctc cttcctcgcc gtttatgacg gttcctgctt
     8881 tgatcctgta caggctgtca tcaataattc tctgcccaat tacaatgaag acgtcctgcg
     8941 tctgaccacc ggctgctcgg tcattgtgac gggtaaagtc gtggcatcgc cgggccaggg
     9001 gcaacaattt gaaattcagg ccagcaaggt tgaagttgct ggttgggttg aagatccaga
     9061 cacttacccg atggcggcaa aacgccacag cattgagtat ctgcgtgaag tcgctcacct
     9121 gcgtccgcgc acaaacctga ttggtgccgt cgcgcgcgtt cgccatacgc tggcgcaggc
     9181 gctgcatcgc ttctttaacg agcagggatt cttctgggtt tcaacgccac tgattaccgc
     9241 atctgatacc gaaggtgcag gcgaaatgtt ccgcgtttct acgctggatc tggaaaacct
     9301 gccgcgtaac gatcagggca aagtggattt cgacaaagac ttctttggta aagagtcttt
     9361 cctgaccgta tctggccagt tgaacggcga aacctacgct tgcgcattat ccaaaattta
     9421 taccttcggc ccgactttcc gtgctgaaaa ctccaacacc agccgtcacc tggcggaatt
     9481 ctggatgctg gagccggaag tggcgtttgc taacctgaac gatatcgcgg gtctggctga
     9541 agccatgctg aaatatgtct tcaaagcggt tctcgaagaa cgcgcagacg acatgaaatt
     9601 cttcgctgaa cgcgtagata aagatgccgt ttcacgtctg gaacgcttca ttgaggccga
     9661 tttcgcgcag gtggattaca ccgacgcagt gaccattctc gaaaactgcg gcaggaagtt
     9721 tgaaaacccg gtttactggg gcgtcgatct ctcttctgag catgagcgtt atctggcgga
     9781 agaacacttt aaagcaccgg tagtggttaa aaactatccg aaagatatta aagcgttcta
     9841 tatgcgcctc aacgaagacg gtaaaaccgt tgcggctatg gacgttctgg ctccgggcat
     9901 cggtgagatc attggtggct cccagcgtga agaacgtctg gacgttctgg acgagcgtat
     9961 gctggaaatg ggcctgaata aagaagatta ctggtggtat cgcgatctgc gtcgctacgg
    10021 tactgttccg cattcaggtt tcggtcttgg ttttgaacgc ctgattgctt acgtaactgg
    10081 cgtgcaaaac gtgcgtgatg tgattccgtt cccacgtact ccgcgcaacg ccagcttctg
    10141 attttcttcc tcaaagagcc agcgcctgct ggctcttttt tatcctttct gtcaagttat
    10201 ctgtttgtta agtcaagcaa tctatttgca accccgccat aacgatcatc ctgttacgga
    10261 atattacatt gcaacattta cgcacaaaaa ataacccgca ttcttattgc ggattagttt
    10321 tttcttagct gatagcacaa ttttcatact attttttggc attctggatg tctgaaagag
    10381 gattttgtgc caggtcgata aagtttccat cagaaacaaa att
//
LOCUS       DAZRUF010000148        10268 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZRUF010000148 DAZRUF010000000
VERSION     DAZRUF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10268
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.148"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHXOL_002943"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHXOL_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR6809874.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHXOL_002944"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHXOL_002944"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR6809875.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHXOL_002945"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHXOL_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR6809876.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHXOL_002946"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHXOL_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR6809877.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHXOL_002947"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHXOL_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHXOL_002948"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHXOL_002948"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR6809878.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHXOL_002949"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHXOL_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR6809879.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHXOL_002950"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHXOL_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR6809880.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHXOL_002951"
     CDS             complement(9457..10149)
                     /locus_tag="ACHXOL_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR6809881.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2499 a         2474 c         2720 g         2575 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaa
//
LOCUS       DAZRUF010000149         9999 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZRUF010000149 DAZRUF010000000
VERSION     DAZRUF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.149"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            11..904
                     /locus_tag="ACHXOL_002952"
     CDS             11..904
                     /locus_tag="ACHXOL_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR6809882.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            1039..2259
                     /gene="mlc"
                     /locus_tag="ACHXOL_002953"
                     /gene_synonym="dgsA"
     CDS             1039..2259
                     /gene="mlc"
                     /locus_tag="ACHXOL_002953"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR6809883.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2384..3079
                     /gene="bioD"
                     /locus_tag="ACHXOL_002954"
     CDS             2384..3079
                     /gene="bioD"
                     /locus_tag="ACHXOL_002954"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR6809884.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3032..4324)
                     /gene="clcB"
                     /locus_tag="ACHXOL_002955"
     CDS             complement(3032..4324)
                     /gene="clcB"
                     /locus_tag="ACHXOL_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR6809885.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4483..5097)
                     /gene="dmsD"
                     /locus_tag="ACHXOL_002956"
     CDS             complement(4483..5097)
                     /gene="dmsD"
                     /locus_tag="ACHXOL_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR6809886.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5140..5994)
                     /gene="ynfH"
                     /locus_tag="ACHXOL_002957"
     CDS             complement(5140..5994)
                     /gene="ynfH"
                     /locus_tag="ACHXOL_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR6809887.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(5996..6613)
                     /gene="ynfG"
                     /locus_tag="ACHXOL_002958"
     CDS             complement(5996..6613)
                     /gene="ynfG"
                     /locus_tag="ACHXOL_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR6809888.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(6624..9047)
                     /gene="ynfF"
                     /locus_tag="ACHXOL_002959"
     CDS             complement(6624..9047)
                     /gene="ynfF"
                     /locus_tag="ACHXOL_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFR6809889.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVEYDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPAYTPDFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(9108..>9999)
                     /locus_tag="ACHXOL_002960"
     CDS             complement(9108..>9999)
                     /locus_tag="ACHXOL_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR6809890.1"
                     /translation="ADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPI
                     YWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRK
                     DPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYAS
                     TFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIAT
                     GDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLR
                     PSPLAKGNPQHTNLVEIEKI"
BASE COUNT         2454 a         2554 c         2593 g         2398 t
ORIGIN      
        1 tgttttaaat atgaatattg aacttcgtca tctgcgttac tttgttgctg ttgcggaaga
       61 gctgcatttc gggcgcgccg ctgcccgcct gaatatttcg caaccaccgc taagtcagca
      121 gattcaggca ctggagcaac aaattggtgc ccgactgctg gcacgaacta atcgcagtgt
      181 attgctgacg gcagcaggaa aacagtttct tgcagatagt cggcaaatcc tgtctatggt
      241 agatgacgct gccgctcgcg ctgaaagact gcatcagggt gaagcggggg agttgcgcat
      301 tggttttact tcgtccgctc cttttattcg ggcggtgtcc gatacgttat cgctgtttcg
      361 ccgtgattat cccgatgtcc atttacaaac ccgcgaaatg aacactcgtg agcaaatcgc
      421 tccgctcatt gaaggaacgc tagatatggg attgctgcgt aacacagcgt taccggagac
      481 gcttgaacac gcagtcatcg tccatgaacc gcttatggcg atgatcccgc acgatcatcc
      541 cctggcaaat aacccgaatg taacgctggc tgaactggcg aaagaaccct tcgtcttttt
      601 tgatccgcac gtcgggacag ggctgtatga cgatattctc gggctgatgc gacgttacca
      661 tttgacgccc gtcatcactc aggaggtggg cgaggcaatg accatcatcg gtctggtttc
      721 cgccggtctg ggtgtttcaa ttttgcctgc ttcatttaaa cgtgttcagc tcaacgaaat
      781 gcgctgggtg ccgattgctg aagaggatgc ggtttctgaa atgtggttgg tctggccgaa
      841 acatcatgaa caaagtccag cttcgcgtaa ctttcgtatt catctgctga atgctctcag
      901 gtgagggaaa tttcagcgaa aaagcccgaa aaatgtgctg ttaatcacat gcctaagtaa
      961 aaatttgacg acacgtattg aagtgcttca ccatagccta cagattattt cggagcgcga
     1021 aaatataggg agtatgcggt ggttgctgaa aaccagcttg ggcacattga tcaaataaag
     1081 cagaccaacg cgggcgcggt ttatcgcctg attgatcagc ttggtccagt ctcgcgtatc
     1141 gatctttccc gtctggcgca gctggctcct gccagtatca ctaaaattgt ccgtgagatg
     1201 ctcgaagcac acctggtgca agagctggaa atcaaagaag cggggaaccg tggccgtccg
     1261 gcggtggggc tggtggttga aactgaagcc tggcactatc tttctctgcg cattagtcgc
     1321 ggggagattt tccttgctct gcgcgatctg agcagcaaac tggtggtgga agagtcgcag
     1381 gaactggcgt taaaagatga cttgccattg ctggatcgta tcatttccca tatcgatcag
     1441 ttttttatcc gccaccagaa aaaacttgag cgtctaactt cgattgccat aaccctgccg
     1501 ggaattattg atacggaaaa tggtattgta catcgcatgc cgttctacga ggatgtaaaa
     1561 gagatgccgc tcggcgaggc gctggagcag cataccggcg taccggttta tattcagcat
     1621 gatatcagcg catggacgat ggcagaggcc ttgtttggtg cctcacgcgg ggcgcgcgat
     1681 gtgattcagg tggttatcga tcacaacgtg ggggcgggcg tcattaccga tggtcatctg
     1741 ctacacgcag gcagcagtag tctcgtggaa ataggccaca cacaggtcga cccatatggt
     1801 aaacgctgtt attgcgggaa ccacggctgc ctcgaaacca tcgccagtgt ggacagtatt
     1861 cttgagctgg cacagctgcg tctcaatcaa tccatgagct cgatgttaca tgggcaaccg
     1921 ttaaccgtgg actcattgtg tcaggcggca ttgcgcggcg atctactggc aaaagacatc
     1981 attaccgggg tgggcgcgca tgtcgggcgc attcttgcca tcatggtgaa tttatttaac
     2041 ccacaaaaaa tactgattgg ctcaccgtta agtaaagcgg cagatatcct cttccctgtc
     2101 atctcggaca gcatacgtca gcaggccctt cctgcgtata gtcagcacat tagcgttgag
     2161 agtactcagt tttctaacca gggcacgatg gcaggcgctg cgctggtaaa agacgcgatg
     2221 tataacggtt ctttgttgat tcgtctgttg cagggttaac attttttaac tgttctacca
     2281 aaatttgcgc tatctcaatt tgggccagga aagcataact tagactttca aggttaatta
     2341 ttttcctagt ttatatttgt gaagcataac ggtggagtta gtgatgctgg agcgtttctt
     2401 tattaccggt acagacactt ctgtagggaa aacggtggtt tcccgcgcat tgctacaagc
     2461 gttagcctcc cagggaaaaa cagttgcggg atataaaccc gtagcgaagg ggagcaaaga
     2521 gacacccgaa gggctgcgta ataaagatgc cctggtgttg cagagtgttt caaccatcga
     2581 actgccttat gaagcagtta atcctatcgc gttaagcgaa gaagaaagta gcgtggcgca
     2641 cagttgccca atcaattaca ccctcatttc aaacggcctg gcaaacctga ccgataaagt
     2701 cgatcatgtc gtggtagaag ggactggcgg ctggcgcagt ctgatgaatg atttgcgtcc
     2761 actctctgaa tgggtggtgc aggaacagtt gccggtgttg atggttgtcg gtattcagga
     2821 aggttgcatt aaccatgcac tgctaacagc tcaggcgatt gccaacgacg ggctgccgct
     2881 cattggctgg gtggctaacc gaatcaaccc aggactggcg cattatgcgg aagtcattga
     2941 tgtgctgggt aaaaaacttc cggcaccgct cattggtgaa ctgccttatc tgccgcgcgc
     3001 cgaacagcga gaactggggc aatacatccg cttagctatg ctgcgcagtg tgctggcggt
     3061 agatagagtc acggtgtaac gtccgcgaaa ttaccgacgc aatgacgcag gcaatcaata
     3121 aaccggggag tagctgatac tccccggtca tttcacatat catcaacgtc gacataatcg
     3181 gcgcgtgcgt ggttgccgcc aacagtgtcg ccatcccggt caatccgagt aaaagcgtaa
     3241 tttcttcgcc atcagggaac cataatccca ggctacgacc atacaacatg ccaatggcaa
     3301 gaccgataaa tagcgtcggt gtaaagacac caccgggtgc gccggaaccg ctactcgcca
     3361 gcacggcaca cagtttacaa aggaagatcc cggcaatgat cattaacagt ggtggggcgg
     3421 ttaaaaagga ttgtacggtg ctatagccgt tgccccacac tgcaggtgtg aacagggaaa
     3481 gcagacccac gatcaacccg cctagtgcca gttgccaggg cggcgcaagt ttgagactca
     3541 caaatccacg atgacaggcg ttcattaacg ttaacaacag tggtccacac agacctgcca
     3601 gcacacctgt actgataatc agcgcatagt cactagcctg aaccgttact gagagttgta
     3661 cgctgtagag taacgcgtcg ctatgattaa taagattgct aaccagcaac gccacgacgg
     3721 cggaaataat caccgggccg agagaggcca acatcatagt gccaaacagc acttcggcta
     3781 taaataaact gccagcaagc ggggcgcgat aggccgcagc cattcccgcc gcggccccac
     3841 aggcgatcca taatttccat tcctggcgtg gcgtaaaacg ttgggcaaaa caggaggcgg
     3901 caagggcagc taaaagaatc atcgcacctt cgcgaccaat tgcactgccg ctggttacta
     3961 ccagcagcga ggcaagcgat ttaaccaggc ttgctgcgta atcgaactgt ccatcggttt
     4021 gcaacgcttc catgtaatcg gtcggcgcat gagggcgttg ttgggtaaat ttctgccagc
     4081 ccatcagcaa caaacccgcc gccagtccgc cgagcgccgg agttagcagc cgtcgccagg
     4141 gggaaaggtt tgtcgctgca ttgaccagac tgccggagtc attattgagg aacaaccact
     4201 ccagtagcag catcgcatga cgaaacccgg caacggcaaa ggccgcgaga ataccgacga
     4261 ctgttgcgat aagcagacgg cggaacatag tgcgcagatc cggatagatg tgtagatgat
     4321 gcatgagcag aaatgggcaa tgaaacacgg gcgactattt tgcggcaaaa gaggccaacc
     4381 gcaaaagcag tgcgtgcttt aagagataaa aaaacgtgac attgtaactt attattgccg
     4441 aatcgcgcgt aatcgtcacc atccggcaat attacggtga tcctatcgaa agagtggttt
     4501 aaccgcgaca ggaatcaaca gttgtgattg ccattgcgcc agcgttaagc gagccagttc
     4561 acccagtgca cggtagaaag ggtgttctgc tttttcgata aaaacatcaa gaaaacgtgt
     4621 tgaccacgga aaaaggtgcc atgccagcaa ttcttcgcat tctgtctggc gaccattctc
     4681 cgccaaccac gcagccatca acagcagtga accaaaatga tcttccggtt cgttttgctt
     4741 catttcaaac tgaatgcctt tctcgcgcat ccactgacga agtgccaatg ttgaatcgcc
     4801 aaacagcaca gattcgcgat ccagccagac cgatccccac ggcggagacg gcagtgccca
     4861 cgggccgaca aacaaacgct gccaggcctg ggcgtgagtc tcttcacact gtgtctgaaa
     4921 agccgtcacc agcggcgcta atgacgcttc tggtagaggc cactgagttt cccagccgtc
     4981 actggtgagt accgcaacca gaggtgcggc ttccgcactc tctggagcgt aataaaacag
     5041 cgcgcccaac acgcgcgccg cgacagaaaa attatcttgc tgtgaaaaat gggtcattcc
     5101 taacatcctg aaatgtgcgg gttgccccgc accaatttgt taacctgcaa tagccattcc
     5161 tactgtcatg tgcaggccat aaaagagcac acgcccgatc atctcaccaa caagtatgag
     5221 gattagcccc agaacaagtc cggcaacgtg aggttcacgg cgacgaatga gcggacaaag
     5281 ccagcatcca agacctgcgc ataacaatac cacacgccag acctgtaagg aggcgtaatc
     5341 tggtaccagt gcgctggctt gttgcacgga tgagtgaata gacgccagag acaaaccttg
     5401 cagcacaatc accccggcac aagcgatcag cgcgagaacg ctaatgatgg caaatggtgt
     5461 ggtattaaat gtaacgcggg ctgcccgcag aattgcggca gcaagtattg gaccgctcaa
     5521 caataccgtc aggaagaaag ccagcgtagt gtaaccgtta tgccaggttg gcacagtgtc
     5581 gatttgatac acgcaggtca tcatccagac gaaaatgacg ccgagcgcca tgctgacaag
     5641 taaccagagt ttccccaacg cttgcggcat tttaccgatg accgccacca gccaccatag
     5701 tccgccaacg gcaaaaaata tggaaccggc agcaatctcg ttactcagac cagaagctcc
     5761 gattcgattc agcgagttaa acgcccgcag tggcgatccc aggtgcatga cagaggccat
     5821 aaacccgatg cccatcaaca gccagaggaa aaacatgcca cggacaatac gctgacggtc
     5881 tgcgtcattt ttggcggcga accagccaat tccgctaaca atcagcgcgc ctactacgca
     5941 ttgacccaat acggtaaaga taaccagtgg ccactcatgc catccatttc ccattttaca
     6001 cttcctccgg attagccaga taaccagtgg tatcgctggt cgggcggctg ttggcgttag
     6061 gtttgataac gatattgggt tttgtgaaat gcgcgcgcgg cagtggcgcg acggcagcca
     6121 gagtgccgtg cttctggcgc agctcttcaa ttggaccgaa ctcaagtgca cgcagcgggc
     6181 aggattcgac acatatgggt tgtttcccct cggcgacgcg cgaataacaa ccatcgcact
     6241 tcgtcatgtg ccctttttca gcattgtact gtggcgcgcc gtacggacaa gccatgtggc
     6301 agtagcggca gccgatacaa acatcttcat caaccaccac aaagccatct tcacgcttat
     6361 gcattgcgcc gctggggcag acttttgtac atgcggggtc atcgcaatgg ttacaggaga
     6421 tggagagata ataagcgaac acgttctggt gccagacgcc attatcttcc tgccagtcgc
     6481 cgccagcata ttcataaata cggcggaaac tgacttccgg gccaagatct ttaaaatctt
     6541 tgcacgccag ttcgcaggtt ttacagccag tacagcggct ggaatcgata aaaaatccat
     6601 attgtgtggt cacgggctac tccttaaacc ttttcgatct ggacaagatt gctgtgcgac
     6661 gggtttccct ttgccagcgg tgaagggcgg tgagaggtca gaatattgat actgccgccg
     6721 tgatcgaccc ggtcaccaaa catatccgct ttaagccaag caccttgccc gatggcggta
     6781 acgccaggca gaatacgcgg agtcactttt gcggcaatca gcatttctcc attattgtta
     6841 aatacccgca cggtatcgcc atgacggata ccgcgtgcct gagcatcaat ggggttgatc
     6901 cacacctctt gtgggcaggc ctgctgtaac acatcaatat tgccgtagct ggagtgggta
     6961 cgcgctttgt aatggaagcc cgttaactgc agtggatagg ttttccgcag gggatcgtcc
     7021 cagccatcaa aatctggggt atacgcagga aggggatgaa taacttcatc ttttttcaat
     7081 tcccaggtat ccgcaatctt cgccagtcgt tcagaataaa tttcgatttt ccccgaaggt
     7141 gttttcaacg ggtttgcctg tggatcttca cggaatgcgc ggaaagcgac gtagtgttct
     7201 tccgggcatt ttttcttaaa gatcccggtc gttttcatct cctcgtagtc gggcatctca
     7261 gggttacgtt ccttcgtttt cgcatggaga tatttgatcc attcatgctg actgcgacct
     7321 tcagtaaagg tttgataaac gtctggtcct aagcgtttgg cgacttcact cagcatccag
     7381 tagatgggtt tgcgttcaaa ttttgctgag gttgcgggtt gggcgaggat cacatagccc
     7441 atattccctg cagattcatg agagataagg tcttcttgct ctgttggcat caggtcgggc
     7501 aacaggatat cgcaatactt agccgaggcc gtcatgaagt ggtcaatgcc aacaatcatc
     7561 tcgcacttgc tgtcatcctg aagcacctca tgggtgtgat tgatgtcgcc atgttgattg
     7621 atcaatgtgt tactggcgta gcaccataaa aacttgatgg ggacatccag tttttctttt
     7681 ccacgaacac catcacgggt cgcggtcatt tccgtaccat ggtcgatggc atcagtccat
     7741 gtaaagacgg aaatctgcgt tttaacagga ttctcgagca tcgggaacca ttctaccccc
     7801 agatcccagc taccttcgcg tacgcctgag ttgccgccgt ttatgccgac gttaccggtg
     7861 agaacggaaa gcatggcaat agcgcgggat gtttgttcgc cgttggagtg tcgttgtggc
     7921 ccccaaccct gacaaatata agcaggtttt gctgaaccga tctctcgtgc caactggata
     7981 attttttctg ccgggatgct ggtgattttt gctgcccatt ccggcgtttt agctatgccg
     8041 tcaggccctt cgcccagaat ataggcttta taatgcgcgt tacgtggtgc gttggcgggc
     8101 agcgtttttt catcgtaacc aacacaatat ttgtcgagaa atggctgatc gaccatgttt
     8161 tcagtaatca gtacccaggc aatcgcacag gccagtgcgc catcggtgcc agggcgaatg
     8221 ggcagccatt catcttcacg cccggcagct gtgtcgttat aacgtggatc gatgacgatc
     8281 atgcgtgcgt ttgaacgttc gcgggcttgc tcgacgtagt aagtgacacc accaccgctc
     8341 atccgcgttt ctgccgggtt atttccgaac ataacgacca gtttcgtatt ggcgatatca
     8401 tccgggctgt tgccatcatt ggcaccgaac atataactca ttgcggcact gatctgtgcg
     8461 gtactgtagc tgccatagcg actgagaaaa ccaccgcaag agttcatcag acggtacggg
     8521 acgtttgagt tggtgatgtt tccgccatct acacctgttc cgtacaggac atgtacagct
     8581 tcattgccgt aatcttttag gatccgccga agattatcac tgatggtatc cagggcttcg
     8641 tcccaactta tccgttcaaa tttaccttca ccgcgcttgc cgacgcgctt catgggatat
     8701 ttcaacctat caggatgatt catccgtcgg cggatagagc gcccgcgtaa acacgcgcga
     8761 acctgatgat taccgtagac gtcgtcacct gtcgtatcat actccaccca gtacacggtg
     8821 tcatctttca catgcaaacg taacagacag cggctcccgc agttaacggt gcaggaactc
     8881 cagaccgctt tctcttctac cggagcctct gccgcccgaa ccatttggga aaatggcaga
     8941 gtgaaagcac tgcttgccag cgcaaggctg ccaagtgcgg aggttttcat cagacttcta
     9001 cggctgattt cagccttcat gagcgcctct gtggtatgga ttttcatcat tactcactta
     9061 ttgcttttca aacaaaatgt catgccagaa tttatggttg tagtgggtta tattttttcg
     9121 atctcgacca gattagtgtg ctgcgggttt cccttcgcca gtggtgaagg gcgcagagtg
     9181 gttagcgtat tcacacagcc gccatggtcg attttatcgc cagacatatt ggcctcgtgc
     9241 caggctccct ggcccatagc gctaacccca gggagaatgc gtggtgttac tttggctggt
     9301 agccgaactt cgccacgatg gttaaacacc cgcaccatat cgccggtggc aatcccacgt
     9361 ttctgtgcat ctatagggtt gatccacact tcctgacggc aggctgcctt caggacatca
     9421 atgttgccgt aggtcgagtg agtacgggat ttgtaatgga aaccaaacag ttgcagtggg
     9481 aaggtactac gttcagggga gtcccagcct tcaaaggttg aggcataaac tggcagtggg
     9541 cttatcactt catctttttc cagttcccag gtacgggcaa tttccgccag cttgctggaa
     9601 taaatttcaa tcttaccgga aggcgtttta agtggatttg cctcggggtc gtcacgaaat
     9661 gctttgtagg cgacaaaatg accattggga tctttacgct tatagatacc catttttttc
     9721 agttcgtcgt aagacggtaa cgccggatct ttggcaagca ttttggcgta cagatgttgt
     9781 aaccattgtt cctgcgtgcg accttcagtg aacttttgat agacgtcagg tccaagacgt
     9841 ttcgcgactt cactcaggat ccagtaaatc ggtttgcgtt cgaatttttc gctggtgaca
     9901 ggctggagga aaatgagata tcccatgtta ccggcgtagt cgttaggaat aatatcttcc
     9961 tgctcaacgg tcatcaggtc tggcagcaga atgtcggca
//
LOCUS       DAZRUF010000150         9756 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZRUF010000150 DAZRUF010000000
VERSION     DAZRUF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.150"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..161)
                     /locus_tag="ACHXOL_002961"
     CDS             complement(<1..161)
                     /locus_tag="ACHXOL_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809891.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLT"
     gene            239..1333
                     /gene="ddlA"
                     /locus_tag="ACHXOL_002962"
     CDS             239..1333
                     /gene="ddlA"
                     /locus_tag="ACHXOL_002962"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR6809892.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1357..1569)
                     /locus_tag="ACHXOL_002963"
     CDS             complement(1357..1569)
                     /locus_tag="ACHXOL_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR6809893.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1829..2137
                     /locus_tag="ACHXOL_002964"
     CDS             1829..2137
                     /locus_tag="ACHXOL_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR6809894.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2196..3290)
                     /gene="yaiW"
                     /locus_tag="ACHXOL_002965"
     CDS             complement(2196..3290)
                     /gene="yaiW"
                     /locus_tag="ACHXOL_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR6809895.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3303..4523)
                     /gene="sbmA"
                     /locus_tag="ACHXOL_002966"
     CDS             complement(3303..4523)
                     /gene="sbmA"
                     /locus_tag="ACHXOL_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR6809896.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4875..6032
                     /gene="ampH"
                     /locus_tag="ACHXOL_002967"
     CDS             4875..6032
                     /gene="ampH"
                     /locus_tag="ACHXOL_002967"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR6809897.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6033..6656)
                     /gene="iprA"
                     /locus_tag="ACHXOL_002968"
     CDS             complement(6033..6656)
                     /gene="iprA"
                     /locus_tag="ACHXOL_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR6809898.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6744..9638)
                     /locus_tag="ACHXOL_002969"
     CDS             complement(6744..9638)
                     /locus_tag="ACHXOL_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR6809899.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTVTNGGVLDVNTNMNDISS
                     VFATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIA
                     MNSDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLI
                     YIDDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIW
                     NLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVD
                     GNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNN
                     DLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLS
                     NDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSID
                     NGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2510 a         2410 c         2348 g         2488 t
ORIGIN      
        1 gttaacttat aattaagaga aaaaaactca ccgcattgcc atttaagcca ggaataaatt
       61 agcttcccgt tcatcatttt tgtgatctcg cgcacatctt tgagataacg cactttcttc
      121 accagtacgt tgcacgcttt gtcagcagaa gattgcaaca atctttcaga aggccgcgaa
      181 tcccccgttt tgtcgtggta atgtatgcac tttgtgttag acgggatagg tttttaagat
      241 ggaaaaactg cgggtaggaa tcgtttttgg tggtaaatca gcggaacatg aagtgtctct
      301 gcaatcggca aaaaacattg tcgatgccat tgataaaagt cgcttcgacg ttgtgctgct
      361 gggcattgat aaacaagggc aatggcacgt cagcgatgcc agcaattatc tgctaaatgc
      421 agacgatcct gcccatattg cgttgcgccc ttcggcgacc agccttgcgc aggtgccagg
      481 taaacatgag catcagctta tcgacgcgca aaacggtcag ccgttgccga cggtggatgt
      541 cattttcccg attgtccacg gtacgctggg cgaagatggt tccttgcagg gaatgctgcg
      601 ggtcgccaat ttaccgtttg taggttctga tgttctggct tcagcagcct gtatggataa
      661 agatgtcacc aaacgtctgc tacgcgatgc cgggctgaac attgcgccat ttattaccct
      721 gacgcgcgct aatcgtcaca acatcagttt tgccgaagtg gagtctaaac tggggttacc
      781 actgtttgta aaaccggcta atcagggctc ttctgttggt gtcagcaaag taaccagtga
      841 agaacagtac gcaattgccg tcgatctggc gttcgagttc gatcataaag tgatcgttga
      901 gcaagggatc aaaggtcgtg agatcgaatg cgcagttctg ggcaacgaca atccgcaagc
      961 cagcacctgt ggcgagatcg tactcaccag cgatttctat gcctacgaca ccaagtacat
     1021 tgacgaagat ggcgcgaaag tggtagttcc ggcagccatt gcaccagaga tcaacgataa
     1081 gatccgggcg attgccgttc aggcttatca gacgttggga tgcgcaggca tggcgcgtgt
     1141 agacgtgttt ttaaccccag agaacgaagt ggtgatcaac gagatcaaca cactgcctgg
     1201 cttcaccaac atcagtatgt atccgaagct gtggcaagcc agcggtctgg gttacaccga
     1261 tctgatcaca cgtttgattg agctggcgct ggagcgtcac gctgcggata acgcattgaa
     1321 aaccacaatg taatatttta acgcccgata atattcttat tgggcgtttt cagtttcttc
     1381 atcgcgacga cgaataatca accccgccag ccagaaacta atcacccacg tcaccagacc
     1441 gacggcatag gtctgccaac cttttgcttc aaatcccagt aaccccacca cgccattaag
     1501 aatgaatata aggccaatgg cgaacgcata gtagtgccag tcacggcgga tttttacagg
     1561 cagattcatg gcagctccgt taacgaaaaa caacatcctt gcatattctg taacaaccgt
     1621 ctgtggtgta gacaacattt tcgtaattgt taataaattt aacttaaggt tgccattttg
     1681 taactcacaa cgaagattca cgcttcggga atattcccaa catcaattta ccatgattct
     1741 gatattgaca atctaaatga cctgtcagac ttgccataag ccgctggtat cacaccagaa
     1801 gagaagcaga tgacttccgg aggtttttat ggctgatttc accctgtcaa aatcgctgtt
     1861 tagcggaaaa tatcgcaatg cctcttcaac gcctggcaac attgcctatg cgttgtttgt
     1921 gctgttttgc ttttgggctg gggcgcaatt gctgaacctg ttagtgcatg cgcccggcgt
     1981 ctatgagcgt ttaatgcagg tccaggaaac aggtcgccca cgggtggaaa ttggtttagg
     2041 tgtcggcacc attttcgggc tgatcccgtt tttagtaggc tgcctcattt ttgcagtggt
     2101 ggcgctatgg ctgcactggc gacatcgccg ccagtaagat ttacgttgcc agatacggcc
     2161 taaaacgcct tatctggcat gacattcctg tttgatcact gtttcataca acgcgcccgc
     2221 cgttcgtcta cgcgcttcgc aaaccaggcc gtagtcaggt tgcgggtgat tttcgggctt
     2281 tccagttgaa tgccaggcaa catttcgcgg ggtagtgatt tacccgtttt cgcttcggca
     2341 agttgataaa ctttcttgta cagcgccgtc tcctcaaaag agaagctgtc acctttctct
     2401 aactgacggc gaatttcgct gtcgctcatc cccagttttc ccgccagttt gcgcgttgcc
     2461 agttcggttt tcccgggttc tttactgtcg taacgaatta aatcaccgtc cagtgccagc
     2521 ttcacgccgc tggccttact gactgcgttc tgaaacgcgg cattacggct ggcgtaccag
     2581 ccagcgttaa aatcagcaaa gcggtatatc ggtgcgctat agctggcggg ataattcagt
     2641 aaatggtaag taccaaacca caacccgccg cggcgactga agacttcctg acgaactgta
     2701 ccgtccattt tccacggata ccctttggta tgctgttcgg caaaagcaat gctgacctgc
     2761 atcggcccac cggtgcgcac cggattaagc gaaccaaaca gcgtctgccc cattggtacc
     2821 atgctgatca gatcgtcaaa aatcgcgctc aattgctttt ctgtacgcac cgaatctaac
     2881 cgttcgctat aacttttgcc gtttggcgat ttgattttta gcgcggtatg aaccagaaaa
     2941 gcaggaatgt gcatccgttc agcacgacgg tcaatctctt gccaggcgat cttgcttaag
     3001 cccggaacgg ccggatctgc ctgataattt gactcctgct gcgccaccgc cagcaccgag
     3061 cagacgtttt ccagcgtcgg tgccagcccc tgactttcaa aggtgatggc gagatctttc
     3121 gcccaggcat cgcggtcttt cacgctcgca ggcatcttct gccgcacgac gctcgctaca
     3181 tctatcgctt tttcgccctt tttcaacggt tgcggagcct gactactaca ccccgctaaa
     3241 accagaaccg ccaacaatga cagcgaagat agatgattga cgcgcgacat tacttctcct
     3301 ttttagctca aggtatgggt tacttcctga attttgtcac catccagctc atgttcaaag
     3361 ctgcgcagac gtttgtagat agacatcaac tcaaccagtg tggtccatga gttaatcagg
     3421 tactggaaag caccgcgaac ctgaccaaaa acgttggtaa tctgcgtcat caggccgagc
     3481 gtaatcgtac cggcaacaat tgacggaaac agcaagaaca aaccgaaaac gttatcgacc
     3541 tgcaaataga gaatgcgggc gatgttgaaa tacatatagt gaaaatagag gcgaaaatag
     3601 tttttccgta cggcgctaaa cagctcgcgt accgtaggcg gcgtcgcgcg cgtggcatcg
     3661 tcttcaccat aaaccagctc tttacggtag gcggcctcta ctcgctggtt tttaaactcc
     3721 agccccggca gtttgatccc taccactgcc agccatccgg tccccatcag cgaccagacg
     3781 atagcggcaa tcaccagacc atacggaatg tgcccgataa tcggcagctc cggcacatgc
     3841 gcggagagcg ttaccagcac cggcaggaag gcgatcaacg tcatgatggc gttgataaaa
     3901 ctgacgccca tattctccag cgttgaagca aaacgcatgg tgtcttcctg cacacgctgt
     3961 gcggcacctt cgatatgacg cagttgttgc cagttcgcca tgtaatattc gttcatcgcc
     4021 gtacgccagc ggaacacata gtgactgaca aagaagttgt tcagtacact gatcaccacc
     4081 gcgatcagcg caatccccag aaagacgccc acttcgcggt aaaattgttc gatagtgact
     4141 ttatgcggcg aacttagcgc ggtttgaatc aaatcataga acggtgcata ccaggcgttg
     4201 acggcgaccc cgacttccac caaaaaccag gtgacgaaga tgatcagtgc agtaccgagt
     4261 attgaccagt attgccaacg gtgcgggctg tagataaacc agaaaaatgc aaaaagtcct
     4321 acgcaaacaa tgtagtaagc gtaaaaaatc aggaaatcca acgaccagaa acgcgcggcg
     4381 ctaatcggga tctgcccgga agcgccggtg atacgcgcca cccagtctcc cccaccggct
     4441 tgccagaaga taacggcaat caatgcccaa acaaaggccg agagaaaaaa cgctcccggc
     4501 tttgggaaaa aagacttaaa catatgcact cctgctaact tcttatcgtt ttaacaactg
     4561 ctgtgtatta accgcatgac cactacaaag cgtcagccaa atgaaaccgc ccattgtgac
     4621 ccatagcaag gcgcttagtt cgccagcctg accataagaa ttgtttcgac ggatttcaca
     4681 aaccaaagat tgtttacata agcggattaa cacgcgcagg atcacaaatg caggcattgg
     4741 tgagcaggaa aaagatcgtg tttacgcgcc gttacgtttt taacactatt attgcactaa
     4801 ttcgcccttt gcaatccatc aatgagtagt ataaatacgc tcagttacct tcattcaatc
     4861 tatggacacc accgttgaaa cgtagtctgc ttttttctgc cgtgctgtgt gcggcgtcat
     4921 tgacctccgt ccatgcggcg cagccgatca ctgaaccgga gtttgcctct gatattgtcg
     4981 atcgttatgc cgatcatatt ttttacggca gcggtgcaac ggggatggcg ctggttgtta
     5041 tcgacggtaa ccagcgcgtc tttcgtagtt atggcgaaac gcgacctggt aataacgttc
     5101 gcccgcagct ggattcggtc gtgcgtattg cttccctcac caaactaatg accagtgaaa
     5161 tgctggtgaa attgctcgac caggggaccg taaagttaaa cgatccgcta agtaaatatg
     5221 ctccgccagg cgcacgcgtg ccgacctaca atgggacgcc cattacccta gtgaatctgg
     5281 caacccatac cagcgccctg ccccgtgaac agcccggtgg cgcggcacat cgtccggtgt
     5341 ttgtctggcc aacgcgcgag caacgctgga aatacctttc tacggcgaag ctgaaagccg
     5401 cgccaggcag tcaggcggcg tactctaacc ttgcgttcga cctgctggca gatgcactgg
     5461 cgaatgcgtc cggcaagcct tatacccagt tgtttgaaga gcagattacc cgtccattgg
     5521 ggatgaaaga caccacctac accccctcac cggatcagtg ccgccgttta atggttgctg
     5581 agcgcggtgc cagtccgtgc aataacacgc tggcggcaat tggcagcggc ggcgtctatt
     5641 ccacgcctgg cgatatgatg cgctggatgc agcagtattt gtcatctgat ttttatcagc
     5701 gcagtaacca ggcagatcgc atgcagacgt tgatttatca gcgcgcgcag tttacgaaag
     5761 tgattggcat ggatgtcccc ggcaaagcgg atgcgcttgg tttaggctgg gtatatatgg
     5821 ccccgaaaga gggtcgtccg gggattattc agaagacagg cggtggtggc ggtttcatta
     5881 cctatatggc gatgatcccg cagaagaata ttggcgcgtt tgtggtggtc actcgttcgc
     5941 cgttgacgcg ctttaaaaat atgagcgatg gcatcaatga tctggtgacc gagctaagcg
     6001 ggaataaacc gttggttatc cccgcatcct gattaatact ctgaaggcaa acggttgata
     6061 gcgaccagtt tgcctttatt catttcgata tagccgcctt tacgcaaagc agcgagaact
     6121 tctgcgacga cagaacgcga tatgcgtgta cgttgatgga tataattcat cacaccaata
     6181 cgtgatcgca attcttcatt ccagtcaatc attgataata atgttgcgcg gatttgttcg
     6241 taggaattat gcccaatgag ctgcacgtcg cgtaattcca gaattctatt ttgccaggct
     6301 aaccagtaaa aagcgtctcg ccagagttga ttttgttcaa taagcgtaat ggtttgtttg
     6361 gctggtagat gatatcccgt acaatttcct tctgatatta atttgtatgg tatatcgttt
     6421 ttcattaaac catcagccag ccccataata taaggggcct gggtaatacc gataagtacg
     6481 ttttcttctc tacgcagaga gataactccc tctaaaataa cgaaagtatc ttcgttattg
     6541 acatcactgg aaaatataac ttgcttttca ttattaaact cgaagcgcgt accgtatctg
     6601 gacaaacatt tatcgagctt accaaattcc tgaagaggtt taactacaga taacatttgc
     6661 gcgtcctttg cagtaatgcc cgtcaaatcc ttgacgggca ttatttagat taaattacca
     6721 gtatttcttc ggagtgaaga atattaccag gtatatttaa cacccacgtt cgcggaccag
     6781 tcttgatcta cgtcaccacc accgaggtag ttagcatcgg tataggcgct gaagttcttg
     6841 gtgaagctga actgagtacc cagaccaaca cgtaccgcag acccttcagt accgttatcg
     6901 atggaatcac cgttcacatc gttatcgttg ttagagtcgt cgtagacgta agccagtttg
     6961 aagtacggag tcagagcctg atcttcgctg taggtgaagg tataacctgc atctacaccc
     7021 agttcataac gcatgctgtc gtaagactga ccgtcaactt tcatgtcgtt gctcagctgg
     7081 tagtcatcac cagactggaa cagaccagaa acgctgccgt aaggagtcac gtaaccagca
     7141 tcacccagtt tgaagtcgta accggctttc aagccgaagc cccaagcgtc ggagttggtg
     7201 ctaccgtcaa cgtaagtacc gttgctcatg gttgcagaca ggtcgttgtt gaagtgagag
     7261 tagctcaagc taccatcaac aaagacgttg ttcgcgaagt gagcagaaga gtagatgtag
     7321 gcagtctggc tgtcttgatc tacctgacca gaacggtcat tcatgtcacc tttagcgaag
     7381 cctgcagccg caccgacgat ccacttagcg ttgttaccgt caactttggt atcaacaccg
     7441 accatgatgc cgttaacatc ctgatcatag ttgatggtgc cgttgtcgcc gttgaagtta
     7501 ccaccgaagt agcttaccca tgcgccgccg ttatcagcca ggccatgacg agagttggtc
     7561 agacgagtac caacggtgtc ttgttccagg ttccagatat tggtgttcgc agacgggatg
     7621 ctcagcgcca tgttagcgta gtcggtcagc tccatctgtt gcagaacaac ggtgttaccg
     7681 cgctgttcag cctgataggt gtatgcaccc aggtcagctt tgttagcagc agagaaggtc
     7741 gcgttggttt tggtatcatc gatatagatg agctgcttac ctgcgtaatc agcaatcgca
     7801 ccagcgccag tggagttgtc gatacgaact ttgtagttac cggttgcagc tacaacgttg
     7861 tctgcagagc tgttgtcaac ttcgtcgcca ttgttaatgt cgttgttacc attaacggtc
     7921 aagtgaccat cagagttcat cgcaataaca ccataaccgt agttagcttt gctggtgtcg
     7981 ttggtacgat cgttgacgag gtctgcattc aggatgtaat cattactatg aatgtcaaac
     8041 acacctgcgt aaacattacc gttgttatct ttaacgttgc cactggtcag ttccagagta
     8101 tcagttgcaa atacgctgga gatgtcattc atgttggtgt ttacatccag cacaccaccg
     8161 ttagtaacgg taacggtaat ggtattggca tacagcggtg catatgccca agctgaagca
     8221 gtatcttcac cgaagttaac ggttgagaag gtatcaatgg tcaggtgatc ggtgttaact
     8281 tcaccgttaa cttccattga agaaccaccg ttcagagtga tggtgtcaga agtcagagaa
     8341 gaatctgcac tcacgttaac ctgagaaccg ctgttgatcg ccagggtatc gatcagggaa
     8401 gttttcgtgg tttcccaatc agaaccgttg ttcagaacaa catcgaatac gccgctctgg
     8461 aatgcgttag cgttatcaac acgattccag ccatcttcag agtaagaagc gtacgggttc
     8521 aggctgtttg gtgcaacgtc gtagaaggct tcgtcagcaa caacaacgtc ataatcaata
     8581 ttcgctgaac caacccattt ggtgttgttc agagtcaggt tcaggtgatc gacgttctga
     8641 tcgtcagttg cccaaccgcc atgagtgtaa gcagtggtag agttgtcaat caggtgaccc
     8701 gtcgcgtcaa agttagcgtt ccaggtactg tcaaatacaa cgtcaccaag caactgagag
     8761 ttgttgaacg taacgttgtt gaccatacga taatcagaag tgtcatcatc gatgaaggct
     8821 aacgcaacat catcagcagg agatgcgcca ttgccgtagt cacttggttc aacagaattg
     8881 ccgaaatgac cacgatctga ttgttcgtct tcagtccatg aaccagaagt tacagtagag
     8941 tcattaacaa cgatgttgtt gtcccactga tgaccttcgg tattgtaggt cgcattaccg
     9001 aggtcgtgtt gaaccaaagt aatacctgca acatgggagt tgttgctgat gttgatgtta
     9061 ctttcaacat ccagagtcac agcggtacca agaccagcgg ttaccaaacg atcataatct
     9121 gtcttagatg ttttaccgtc tgcatccaga taagtatcgg tgaagtagaa ggcgtcaaaa
     9181 tcatcgtcaa tagtagaatt agcgatgttc agagtgaaga tgtcgccatc aacccagtcg
     9241 tggccagtat aatcgctacc gttggtccag acgtagtcgc cataaccaac ctggtaagat
     9301 gtaaccatac catgaacaat tgagttggag atgttcagtg tattaccggt atctgtgcca
     9361 taatcgtggt tcagataata agtagaaatt acaccattta cagtgtcgcc gctgaaagtg
     9421 gtatcaaagt aaccatcagc tgcgtggtca tatacataac caccgtaagt cagataatcg
     9481 tgatcaattt tagtgctgtc accccaattg tagatggtgg ctgcgtcatc gtgatacccg
     9541 aaggtgttat aagtagctgc attagcctga gaggtgatag ccagagtgca agccaatgct
     9601 aattgtgata ctacaagttt ctttttccag gagtgcattt gtcatccctc ctcagggacg
     9661 taataagttg atccatcaat tatcaatgca tagaaaacat attatgtttc gcgctaacga
     9721 ttatgctgcg ttacagacga atggttcaat tattct
//
LOCUS       DAZRUF010000151         9736 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZRUF010000151 DAZRUF010000000
VERSION     DAZRUF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.151"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            79..693
                     /gene="evgA"
                     /locus_tag="ACHXOL_002970"
     CDS             79..693
                     /gene="evgA"
                     /locus_tag="ACHXOL_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR6809900.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            698..4291
                     /gene="evgS"
                     /locus_tag="ACHXOL_002971"
     CDS             698..4291
                     /gene="evgS"
                     /locus_tag="ACHXOL_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR6809901.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKNN"
     gene            complement(4347..5492)
                     /gene="yfdE"
                     /locus_tag="ACHXOL_002972"
     CDS             complement(4347..5492)
                     /gene="yfdE"
                     /locus_tag="ACHXOL_002972"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR6809902.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5566..6510)
                     /gene="yfdV"
                     /locus_tag="ACHXOL_002973"
     CDS             complement(5566..6510)
                     /gene="yfdV"
                     /locus_tag="ACHXOL_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR6809903.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6580..8274)
                     /gene="oxc"
                     /locus_tag="ACHXOL_002974"
     CDS             complement(6580..8274)
                     /gene="oxc"
                     /locus_tag="ACHXOL_002974"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR6809904.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8328..9578)
                     /gene="frc"
                     /locus_tag="ACHXOL_002975"
     CDS             complement(8328..9578)
                     /gene="frc"
                     /locus_tag="ACHXOL_002975"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR6809905.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2884 a         2225 c         1998 g         2629 t
ORIGIN      
        1 ttgagaaaat gagatgacgc cttatgtctg tattactaca gggagaaggg agatgcttca
       61 ttgcaaaggg aataatctat gaacgcaata attattgatg accatcctct tgctatcgca
      121 gcaattcgta atttattgat caaaaacgat attgaaatct tagcggagtt aactgaaggc
      181 ggaagcgccg ttcagcgggt ggaaacactt aagcctgata tcgtcatcat tgatgtcgat
      241 atccccggag ttaacggtat cctggtgtta gaaacgctga ggaagcgcca atatagcgga
      301 attattatta tcgtctccgc taaaaatgac catttttacg ggaaacattg tgctgatgct
      361 ggcgctaatg gtttcgtgag taaaaaagaa ggcatgaaca atatcattgc ggctattgaa
      421 gctgcaaaaa atggctactg ctatttcccc ttctctctca accggtttgt tggaagttta
      481 acgtccgacc agcaaaaact cgactcctta tcgaaacaag aaattagtgt catgcggtat
      541 attcttgatg gcaaggataa taatgacatt gcggaaaaaa tgttcatcag caacaaaact
      601 gtcagcactt ataaaagtcg cctgatggaa aaattagaat gtaaatcact gatggatctt
      661 tacacattcg cacaacgtaa caaaatcggc taaccacatg aagtttttac cctatatttt
      721 tcttctctgt tgtggtcttt ggtcgaccat aagtttcgca gacgaagatt acatcgaata
      781 tcgtggcatc agtagtaaca accgtgtcat acttgatcca gtacgtctga gcaacaagga
      841 attacgttgg ttagcgagca aaaaaaatct tgtgattgca gtacataagt cccaaacggc
      901 tacgttgttg cataccgatt cgcagcaacg agttcgtggt attaatgctg attatttaaa
      961 tcttttaaaa agaacgttaa atatcaaatt aacactccgg gaatacgccg atcatcaaaa
     1021 agcaatggac gcgctggaag aaggtgaagt cgatatagtg ttatcacatt tagtagcttc
     1081 gccgcctctt aatgatgaca ttgctgcaac caaaccactg ataattacct ttccggcgct
     1141 ggtaaccacc cttcacgatt caatgcgacc gcttacctca tcaaaaccag taaatattgc
     1201 tcgagtagca aattatcccc ctgacgaggt aattcatcaa tcatttccaa aagcaacaat
     1261 tatctctttt acaaatttat atcaggcatt agcatccgtc tcagccggac agaatgatta
     1321 ctttattggt agtaacatca ttaccagcag tatgatttcc cgctatttca ctcactcctt
     1381 aaatgtagtg aaatattata actcaccgcg tcaatataat tttttcttga ccagaaaaga
     1441 atctgtcgtt cttaatgaag tactcaatcg atttgttgat gctttgacaa atgaagttcg
     1501 ctatgaagta tcacaaaatt ggcttgatac aggaaacctg gcctttctga acaaaccatt
     1561 agaactcact gaacatgaaa aacaatggat taagcagcat cccgatttaa aggtgctgga
     1621 aaatccttac tcccctccct attctatgac ggatgaaaat ggctcggttc ggggcgttat
     1681 gggggatatt cttaatatta ttactttgca aacaggttta aatttttctc cgatcaccgt
     1741 ttcacacaat atccatgctg gaacacagct gaatcccggc ggatgggata tattacccgg
     1801 tgctatttat agtgaagata gagaaaataa tgttttattt gctgaagcct tcataacaac
     1861 gccttacgtt tttgtcatgc aaaaagcgcc tgacagtgaa caaacattaa aaaaaggaat
     1921 gaaagttgcc attccatatt attatgagct tcattcgcaa ttaaaagaga tgtatccgga
     1981 ggtagagtgg ataaaagtcg ataacgccag cgctgcattt cacaaggtca aggaaggcga
     2041 acttgatgct ctggtcgcga cacagttaaa ttcacgttac atgatcgacc attactatcc
     2101 taatgaactt tatcattttc ttattcccgg cgttccgaat gcatcgcttt cgttcgcttt
     2161 tcctcgcgga gaaccggaac ttaaggatat tattaataaa gcactgaatg caatcccccc
     2221 aagcgaagtt ctgcgcctga ccgaaaaatg gattaaaatg cccaatgtga ccattgacac
     2281 atgggacctc tatagcgagc aattttatat tgttacgaca ttatccgttt tattagttgg
     2341 cagtagcctt ttatggggat tctacctgtt acgctcagtt cgtcgtcgta aagtcattca
     2401 gggtgattta gaaaaccaaa tatcattccg aaaagcactc tcggattcct taccgaatcc
     2461 aacttatgtt gtaaactggc aaggtaatgt cattagtcat aatagtgctt ttgaacatta
     2521 tttcactgcg gattactaca aaaatgcaat gttaccatta gaaaacagtg actccccctt
     2581 taaagatgtt ttttctaatg cgcatgaagt cacagcagaa acgaaagaaa atcgaacaat
     2641 atacacacag gtttttgaaa ttgataatgg catcgagaaa agatgcatta atcactggca
     2701 tacattatgc aatctgccag ccagcgatca tgctgtttat atttgtggtt ggcaagatat
     2761 tacagagacg cgtgatttaa ttcatgcact cgaagtagag agaaataaag cgatcaatgc
     2821 aactgtcgca aaaagtcagt ttctggcaac aatgagtcac gaaataagaa caccaataag
     2881 ctccattatg ggcttcctgg aacttctgtc gggttctggt cttagcaagg agcaacgggt
     2941 ggaggcgatt tcacttgcct actccaccgg acaatcactc ctcggcttaa ttggtgaaat
     3001 ccttgatgtc gacaaaattg aatcgggtaa ctatcaactt caaccacaat gggtcgatat
     3061 ccctacttta gtccagaaca cttgtcactc tttcggtgcg attgctgcaa gcaaatcgat
     3121 cgcattaatt tgcagcagta catttcctga tcattacctg gttaagatcg accctcaggc
     3181 gtttaagcag gtcttatcaa atttactgag caatgctctc aaatttacca ccgagggggc
     3241 agtaaaaatt acgacctccc tggttcacat tgatgacaac cacgctgtta tcaaaatgac
     3301 gattatggat tctggaagtg gattatcgca ggaagaacaa caacaactct ttaaacgcta
     3361 cagccaaaca agtgcaggtc gccagcaaac aggatctggc ttaggcttaa tgatctgcaa
     3421 agaattaatt aaaaatatgc agggcgattt gtcattagaa agtcatccag gcataggaac
     3481 aacatttacg atcacaatcc cggtagaaat tagccagcaa gtggcgactg tcgaggcaaa
     3541 agcagaacac cccatcacac tacctgaaaa gttgagcata ttaatcgcgg atgatcatcc
     3601 gaccaacagg ctattactca aacgccagct aaatctatta ggatatgatg ttgatgaagc
     3661 cactgatggt gtgcaagcgc tacacaaagt cagtatgcaa cattatgatc tgcttattac
     3721 tgacgttaat atgccgaata tggatggttt tgagttgact cgcaaactcc gtgagcaaaa
     3781 ttcttcctta cccatctggg ggcttacagc caacgcacag gctaacgaac gtgaaaaagg
     3841 gttaagttgc ggcatgaact tatgtttgtt caaaccgttg accctggatg tactgaaaac
     3901 acatttaagt cagttacacc aggttgcgca tattgcacct cagtatcgcc accttgatat
     3961 cgaagccctg aaaaataata cggcgaacga tctacaactg atgcaggaga ttctcatgac
     4021 tttccagcat gaaacgcata aagatctacc cgctgcgttt caagcactag aagctggcga
     4081 taacagaact ttccatcagt gtattcatcg catccacggt gcggctaaca tcctgaattt
     4141 gcaaaagttg attaatatta gccatcagtt agaaataaca cctgtttcag atgacagtaa
     4201 gcctgaaatt cttcagttgc tgaactctgt aaaagaacac attgcagagc tggaccagga
     4261 gattgctgtt ttctgtcaga aaaataacta aatagcggct cccacaatgt tctaatgtgg
     4321 gagctattta ccagcacatc ttacatttat gatgagaact cctggcgaat ttgttcccca
     4381 tgctggtcga gcgttgccgc tcccggcata acatgcgggt ccgcgcagcc gctgattttt
     4441 atcggattac ccggcatcat tattcctccg gcttcaatca acatatttct cgcctgagtt
     4501 tgtggcaaat taatggcctc agccacactt aataacggcg cgacgggcac accaacttca
     4561 tgtattttgg ctaaccaaac ttcagctgcc tgcgttttta acgttcgctc aatatattgt
     4621 ttaagaatag cctggttttg tacgcgtaaa atattgctgc taaatcgggg atcattaacc
     4681 agttccgtaa gctccagtgc ctggcataac gcagaaaaaa gcttgtcatt accacaacaa
     4741 atcgtaatcg gcttatcctg agtatcgaaa acatcaaaag gtgccatgta gggatggcga
     4801 tttcccaggc gttgtggtga cttccctgtc gcaatatatg ccatcagacc atgctccaga
     4861 aaactcagcg tggcatcaaa catcgctata tcgacatgcg cccctctctg gctcttttcg
     4921 cggccataaa gtgcactcac tattccgctg aataaataga caccgccgca tagatccgca
     4981 agagaggtac caacacgcac tggcggagca tcagggtacc ctgtttccat cattatccca
     5041 ctcattgcct gaatgatggt atcgtaggca ggagcatctt ttagcggacc ggtatgtccg
     5101 aaacctgacg atgaagcata tatgaggcgc gggttgattt cttgtaacct ttcccatgaa
     5161 aaccccagtt tttccattgt acctgggcga aaattctcag ctaatacatc agcttgtttg
     5221 agcatattta taaatatact tttatcgtga tcattcttta aatcaagaac cacactctct
     5281 ttgccatgat taataaaact gtaatagagt gactgtccat ccacataggg accaaatgtg
     5341 cgggtatcat caccatgacc tggtggctca actttaatta cccttgcgcc catattacaa
     5401 agaagttgag ttccgaaagg tccattaagg acatgtgtca tatcgataac taataagcct
     5461 tcaaacggcc ctttgctttc attatttgtc atttgcttat gccttgaaaa aagccgaagg
     5521 tgaaaagaca cttcggcatt tatgtaaaat caataacatg attaattatg aaaccagtcg
     5581 actgacataa atccacaagg gagccgtgac gacaaagccc agtacgctca cagccaatga
     5641 cgctgtacca gtacgggtgt agacattaaa ccggctggca ataatgattc cggagaatgc
     5701 cggcggtaat gcgcctgcca gtaccatcat ctgcaaatgt tcgctgttca aatgacatgc
     5761 cataccaacg agaagcagcg ccagtggcat cagaatcaac ttcaggaagg tgttataagc
     5821 aatttcggca ctgaactcga atttatgtgc agccagagtc aaccccgcag cgaataccgc
     5881 tacccctgag ttagccttcg caatcagatt aaaggtcggg tcccatgctg ccggaatttt
     5941 taccccaacc agcaccagga tcgttgccag aacaggtgcc catactactg gctcctttgc
     6001 cgcagaaatt aatgcgctca gattactatt cttcttacca tccgctcctg aggaaggatt
     6061 cagcaaatac agaccaatag gaatagtaat tgcgttaaca ataatagaaa taattgctac
     6121 cactaaacct gttgatacgg aatcaccata aataggatcg agaactgcaa accccaggaa
     6181 tccaatggta ggtgaacccg caattaatgc acatacagcg gcttctgcat gggtacgttt
     6241 aaaaaatttg tagcaaccaa accaggagaa aaagaaacat ccgacaataa ccacaagtga
     6301 tacaagggtc agacgagtgt ccgcaaaaat catttccctg tttgcccggg taatagaaac
     6361 aaatagagcc gcaggaagag cgtagtttaa taccagttta ttaaatgcgc gggcttgatc
     6421 ttctgaaaaa gtttctcgtc tgccgctaaa ataacccaac agcatgatta cgataatagg
     6481 caataaatcg ccaataaaaa atgttagcat aatagtattt ccgataaggg tcccctcacc
     6541 atcgcataat gagttggcaa gaggagacga tgtcagtatt taattaccag cgacttgttt
     6601 tgggttaagt ttggtaatat ggccactttc agttcctgca gcagggtcga tgaccacatt
     6661 aataatggtc ggtttgcgcg actgaatacc ggtggttaaa gcatgacgaa gttcatctgt
     6721 cgtggtgacg ttatagccaa cgccacgaaa cgcatccatt aatttgtcat accttgcatg
     6781 gtgcaacaga tccgttggtg atggtgcacc agcgccactg agatcaacac cgtctcctct
     6841 gtagatgccg ccattattaa aaataacgat cgtcaccggc aggttatatc gacaaatcgt
     6901 ttcaatttcc atcccactga aaccaaaagc actatcacct tcaatggcga caaccggaga
     6961 accagaagtc acgctagcac cgatggcata gcccataccg atgcccatga caccccaggt
     7021 accacaatcc agacgacgac gtggtttata catatcaata atatttcgtg cattatccag
     7081 ggtatttgca ccttcattaa ctaaataaat atcctggttc tcgcgcaata cgtcgcgcac
     7141 agcacttaat gcattaaagt aatttaatgg ttgagtatct gtacttaatt tttcatgcat
     7201 tttttgtgca ttttgctgct tgtggatatt taaaatatcg cgccatacca gtggagtcgt
     7261 aaatgtgttt tgtttcagtt ctgccagcat accttgcata ctggatgcaa tatcaccaac
     7321 gactggcaca gcaatggggc ggttgctgtc aatttcctgc ggttcaatat ccagttgaat
     7381 aaactgtgta tctgccgccc atcctttttt accgtgtgcc aataaccaat tcagtcgtgc
     7441 accaacaagc atgacaacgt cagcatttgc cagggcaaac gaacgcgcag ctgccgcaga
     7501 aagtggatgc gtatcttcaa ggatcccttt cgccatagac attggcagga atggaatctg
     7561 agcactttca ataaattcac gaagctgttc atcagcttgt gaatacgccg cgcctttgcc
     7621 aaggataatt aatggccgtt cagcttttgc taaaagcgaa attgcgctag tgactgactt
     7681 cgggcatggc aataatgctg gcgacggatt ttcaacttta acaatcgtgg ttaacgcttc
     7741 gtctttttcc atcgtcgcgg ccaggacatt tgctggcaaa tcaagataaa ctccgccagg
     7801 gcgacccgat acagagaccc ggatagcgcg tgccaatgca atgccaagat cctgcggctg
     7861 attaacgcga aatgctgctt tggcatacgg ttttgccgca ttcatttggt ccagctcttc
     7921 ataatcacct tgctgtaggt cgacgatcgc gcggtcgctg gagccgctaa tcatgatcat
     7981 cggaaaacca tttaccgttg cgttggccaa tgcggtcaaa ccattgagga atcctggcgc
     8041 agaaactgtc aggcagatcc ccggtttttg ggtaagaaaa ccgcttgccg cagcggcata
     8101 gcctgccgac tgctcatgac gaaaaccaat ataacgaatg ccttccgcct gggcatggcg
     8161 tgccatatcc gtcacaggaa tacctacaac accataaata gtgtcaatat tattctgttt
     8221 taatgcttca acgatgatat gcataccatc tgtcatttga agttgatctg acatgattaa
     8281 taacctcgaa atttttataa attgggagcc agaagccccc gttaatatca gatggcgtgg
     8341 ttttgcttca ttgcagcaat ttcatcgtcg ctataaccca gctcctgcaa tacagcagcg
     8401 gtatgttcac ctaatagcgg cgcagcttta atatccggcg taaaggcaga gaatttcatt
     8461 ggacaaccaa cggtcagata ttttccacgc aacggttgtt ccacttcaac aacactgcca
     8521 ctttggcgca aagagggatc aagtgaaatt tctttcatac ttaaaaccgg tgcacaagga
     8581 atatcaaact gagtcaaata ggccaccgct tcatgtttat caatagtgac agtgtatttt
     8641 tcgatttcag caaaaatatc gaaaatatgt ggctgtcggg catgggctgt actgtatgcc
     8701 gaatcggtaa tccattctgg tttgccgatg gctttacagg tgttttccca gttttgctcc
     8761 tgaatagtga aataaatata ggcgttagga tcggtttccc agcctttaca tttcaggatc
     8821 cagccaggct gaccgccacc acctgcatta ccaccgcggg gaactgcatc accaaatgta
     8881 ccattcggat actgcgggta ttcttccaga taacccaatt tatcgagacg ctgctggtca
     8941 cgtaatttca cgcggcaaag gttcaatacg gcatcctgca ttgacatggt gactcgttgc
     9001 ccacgccccg ttttttcgcg atgcagcaaa gcagcaagta aaccgatcag caaatgcatt
     9061 ccggtgttgc tgtcacccaa cgctgcagcg cttaccagcg gcggaccatc ccaaaaaccc
     9121 gtagtggatg ccgcgccacc cgctgcctga gcaacgtttt cataggcttt tacattcaca
     9181 taaggcgaac actcatcaaa ccctttgatc gaaccaaaaa tcagacgtgg attgatttct
     9241 tgaatatgct cccaggtgaa gcccatgtga tcaatggccc ctggatgaaa gttctcgact
     9301 aagatatcag cttcgcggat cagcttttcc attacctctt tgccttccgc tgttttggta
     9361 tttaactcaa tagaacgttt gttactgtta agcatggtga agtaaagcgc atcgatatca
     9421 ggaatatctc gcagttggtg acgcgttacg tcaccaacgc cgggacgttc aattttaata
     9481 acgtcagcgc caaaccaggc cagcatttga gtacaagatg ggccagattg cacaccggtg
     9541 aaatcgagaa ctttaattcc ttgaagtgga gttgacatag caatacctat tagttaatta
     9601 aatatgtgtt aatttagcgt ttatttaagg cataacgccc tctataaata atctgcaaaa
     9661 tatcttcgta gcggcactcg atatttttaa attcctgtat caagtactga aaattatttc
     9721 atcgccactt ccaatt
//
LOCUS       DAZRUF010000152         9674 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZRUF010000152 DAZRUF010000000
VERSION     DAZRUF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.152"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..1710
                     /locus_tag="ACHXOL_002976"
     CDS             <1..1710
                     /locus_tag="ACHXOL_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR6809906.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="ACHXOL_002977"
     CDS             1711..3360
                     /locus_tag="ACHXOL_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR6809907.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPMQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="ACHXOL_002978"
     CDS             3365..4141
                     /locus_tag="ACHXOL_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR6809908.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHXOL_002979"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHXOL_002979"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR6809909.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="ACHXOL_002980"
     CDS             complement(5430..6752)
                     /locus_tag="ACHXOL_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR6809910.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHXOL_002981"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHXOL_002981"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR6809911.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHXOL_002982"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHXOL_002982"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR6809912.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIKQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHXOL_002983"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHXOL_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR6809913.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
BASE COUNT         2354 a         2483 c         2605 g         2232 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgatgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgcttg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaa
//
LOCUS       DAZRUF010000153         9648 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZRUF010000153 DAZRUF010000000
VERSION     DAZRUF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.153"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..670)
                     /gene="ydcF"
                     /locus_tag="ACHXOL_002984"
     CDS             complement(<1..670)
                     /gene="ydcF"
                     /locus_tag="ACHXOL_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR6809914.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDS"
     gene            complement(942..4844)
                     /gene="hrpA"
                     /locus_tag="ACHXOL_002985"
     CDS             complement(942..4844)
                     /gene="hrpA"
                     /locus_tag="ACHXOL_002985"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR6809915.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5045..5650
                     /gene="azoR"
                     /locus_tag="ACHXOL_002986"
     CDS             5045..5650
                     /gene="azoR"
                     /locus_tag="ACHXOL_002986"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR6809916.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5701..7017)
                     /locus_tag="ACHXOL_002987"
     CDS             complement(5701..7017)
                     /locus_tag="ACHXOL_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR6809917.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7007..8764)
                     /locus_tag="ACHXOL_002988"
     CDS             complement(7007..8764)
                     /locus_tag="ACHXOL_002988"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR6809918.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8780..>9648)
                     /locus_tag="ACHXOL_002989"
     CDS             complement(8780..>9648)
                     /locus_tag="ACHXOL_002989"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR6809919.1"
                     /translation="ALLILATLINRFLLWRLPERKGGEVTLRIRTWWGIVICFSLVIS
                     GPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNCFELFLLFIPL
                     AGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPSMNIQGGALLV
                     LFLLALTASNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMIASMIIGPLLT
                     PLNTLQAILAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGILDRIDSLIFTA
                     PVFFYFIRYCCY"
BASE COUNT         2448 a         2630 c         2274 g         2296 t
ORIGIN      
        1 cgctatcatc gcgtaaacgc ggcagctcgc cagtgagtag tgagagataa cgctcaaccg
       61 gccataatcc ttgtaactga ttaataaaga ttacactgtc tgcgttattt cctaactgag
      121 gaacgaatcc gggataactt aaccagcgtg gtgcatcggg attgtcccca gtcatacggc
      181 ggaacgtcgc catcgtgcgc cgctgcatgg tggggtcctg aacaacgata gccgtatgaa
      241 ctcgttctac ggcctgattc aatagcgcga tgctaaagcg tgcgttttca ccgcagtttg
      301 ttgactggtc ttcaatccag attttttcat gcggaatgtg ccagaactga tgagcgatat
      361 ccgcctggat ggtcgcttct gctctgccag tggtgcggat agtgttgtag tgcggatgct
      421 gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat accaccacta atcagtaaag
      481 gaatttgctg atcgcgagca atcttacatg ccgcgtcgat agtcggcata accgcattgc
      541 ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc ggagaaatca tcctgcgcca
      601 gccactgtcc gataacattt atggcatcta tagttgccgg cgaaagcgtc ggaaacgggg
      661 taatgttcat ggttcctcct tatcgttccc ttaagaatac tgcgtgagat aatagtgaga
      721 agcggagcac tctgaaagga ggaagtcgta tttccttaac cgccactccg caagcctgac
      781 atatctttta aggaaatgct gtagataaac attattcatc ttgcagcaga gaattgtatt
      841 tttctgaatt cctgaatttt tacccttacc gttttagaat taataaaaaa cccggcgcta
      901 aatgcttacc gggttttttc tttatcaggc aaatagcagg gttaaccgct aatctgctcc
      961 atcgcctgca aaatacgctt atctgaaatc ggataaggcg taccaagttg ttgagcgaag
     1021 taactaacac gcaactcttc tatcatccaa cggatctctt tcacgtcttc atcctcacga
     1081 cgtgcaggcg gcagtttgtt gatccattgc tgccacgcct gctggacgtt ttcgactttc
     1141 agcatctgag cacggtcgcg atgtggatca accgccagtt tttccagccg tttttcaatc
     1201 gcctgcaaat atcgcagcgt gtcgcccagc cgtttgaagc cgttaccagt gacaaaaccg
     1261 cgatatacca gcccgcccat ctgcgcttta atgtcagaaa gccccagcgc catcgtcata
     1321 tccacccgcc ccttcagacg tttgttgata ttgaacactg ccgtaaggat ttgctcgacc
     1381 tgcttcgcaa tatccaccac cgtgtcgttc agttcggcac gcactttttc atgcagcgca
     1441 gcaaagcctt cttccgtcca gaccgggcca ccattggcgt cgatcaattt atccacaccg
     1501 caggagatac aatcgtcgat cagctccagc actttgccat acgggttaaa gtacagcccc
     1561 agcttggctt tgtttggtaa cttctcatgt aaatacttga tcggcgaggg aatattcagc
     1621 agcagtagac ggcgaagacc gttccacatt acctgttttt gctccagcgg attatcaaac
     1681 agtttgatcg ccacactgtc gcgttcatcc actagcgctg gccacgcctt cactttgtag
     1741 ttgccacgct tctgttcgta gctttccggc agctgaccaa aactccagat atgcagtccg
     1801 ctctgctcga taccgtcatc cgccaccgca gacagcgttt cctgcacttt gcctttcagc
     1861 gcatctttca gatcttgtag cgagcgccct tcttttagct tcttgttttt gtcatccacc
     1921 acgcggaagg tgattttcag gtgatcgggc acctgatccc agtgccagtc ttcgcggtca
     1981 acggtaacgc cggtcatccg ccgtaactcg cgctcaaggc tgtcgagcaa cggtaactcc
     2041 agcggtgtga cgcggcctaa aaacgcttcg gcgtagtttg gtgcaggcac aaaattacgg
     2101 cgtaccggtt tcggcaacga tttaatcaga gcaatcacca attcgcggcg cagaccgggg
     2161 atctgccatt caaacccgct ttcctcaacc tggttaagta acggcagcgg aatatgtacg
     2221 gtcacaccgt ccgcgtccgc gcctggctca aactgatagc tcaaacgcag cttgagattg
     2281 ccctgatgcc agaagttcgg gtaatccagc tttctgattt tttccgcccc ttctttgatc
     2341 aacatgctct tttcaaagtt gagcaaatca ggcgtttcgc ggctgacttt tttccaccag
     2401 ctatcgaagt gacgagcgga gattacatcg tggctgatgc gctggtcgta gaactcaaac
     2461 agcgtttcgt catcaaccag aatatcgcgg cgacgtgatt tgtgttccag ctcttccact
     2521 tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac gcgtctgcca gtcaccttcc
     2581 accagagcgt ggcgaataaa gagttcacga cataacgccg aatcgatctg gctgtagttg
     2641 accttgcgcg cagcaacaat cggcaaacca taaacagtga ctttttccgt tgccatcacc
     2701 gcgccctgcg cccgttccca gtgcggttcg ctgtaggtgc gtttaatcaa atgctgagca
     2761 acgggttcta cccattccgg gtcgatacgc gcagcaatgc gcccccacag gcggctggtt
     2821 tctaccagtt ccgccaccat tacccatttc ggcggctttt tgaataaacc agaaccgggg
     2881 aagatggaga aacgcgcgtt acgtgcaccg gtatattcct gtttatcggc atctttcatg
     2941 ccgatatggg aaagcaaacc ggtcagtaag gcgatgtgaa tttcacgata ctccgccggt
     3001 tcgctgttga ccggaatgcc aagttctttc accacctggc gcaactgggt gtagatatcc
     3061 tgccattcgc gcacgcgcag atagttgagg taatcggtac gacacaggcg acggaaggcg
     3121 ttggaagaaa gcgccttttg ctgctcgcca agataattcc acagatttac aaacgcgagg
     3181 aagtcggatt ctttgtcgtg gaagcgacga tgtttttcgt ccgatgcctg ctgtttgtcc
     3241 atcggacgtt cgcgcggatc ctgaatggag agcgcggacg tgataatcat cgcctcacgc
     3301 acgcagccat gtttttgcgc ttccagcacc atacgcgcca gacgtgggtc gacaggcaac
     3361 tgcgagagct ggcgaccgag cggcgtcagt ttataggcgc tggcctgttc atccgtggtg
     3421 atcgcgccca gttcttcgag cagacgcacg ccatcctgga tattgcgttt atccggtgct
     3481 tcgacaaacg ggaacgcagc gatatcgccc agccccagcg cggtcatctg caaaataacc
     3541 gaggccaggt tggtacgcag aatctccgga tcggtaaact ccgggcgcga gaggaaatcg
     3601 tcttcggaat agagacgaat acagatccct tcggacacac gaccacagcg gcctttacgc
     3661 tgattggcag acgcctgcga aatgggttca atcggcaaac gctgcacttt ggtgcgatag
     3721 ctgtagcggc tgatacgcgc tgtaccgggg tcgatgacgt atttaatccc cggtacggtc
     3781 agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgcc gtccgctgtg cgactggaac
     3841 acgcggttct gttcgctgtt cgaaagccgc gcataaagcg gcaagatttc ggtatggcgt
     3901 aagttcagct tgttcagcgc atcggcggta tcgcgaattt cccgctcacc actcataaag
     3961 atcagaatgt cgccagggct ttcctgactc agttcgtcta cggcgtcaaa aatcgcctgc
     4021 aactgatcgc gctcggtgtc atcggcttct tcaacaatcg gtcgatagcg cacttccacc
     4081 ggataggtcc gaccggagac ttcgataatc ggcgcattat taaagtggcg cgaaaagcgt
     4141 tccgggtcga tagtcgcgga agtgataatg atttttaggt caggacgccg cggcagcaac
     4201 tctttcaaat agccgagcag aaaatcgata ttcaggctgc gttcgtgcgc ttcgtcaata
     4261 atgatggtgt cgtactgcat cagcaggcgg tcttgctgga tctccgccag caggataccg
     4321 tcggtcatca gcttgaccat cgtgttatca cttacgtgat cgctgaaacg cactttgtaa
     4381 ccgatgcaac cgcccggctc cgttttcagc tcttccgcaa tacggttcgc caccgttctt
     4441 gccgccaggc gacgcggctg ggtatggccg atcagccctt taatcccgcg ccccagctcc
     4501 atacagattt tcggtaactg agtcgtttta ccagaacccg tttccccggc gacgatcacc
     4561 acctggtgat cacgaatcgc ttcgagaatg tcctgttttt tctgactaac cggtaaattg
     4621 tcaggataag taatttccgg tcgtgccgct tcacgcagca ggactttccc tgccgcctgg
     4681 tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg catcaggatt tttaaccttc
     4741 ttcacgccgt gcagacggcg agaaaaacgc agtctgtcac gcagcatcag cgaatccagc
     4801 cgctgctgca aggccgtaaa ggtcaatttt tgttgttctg tcataacgtt agtgggcagt
     4861 agtctgcctg attaattttc tttgaaatga tagatataga gtaccacatc acggctttct
     4921 atgcgttgtt caataaattc gaacataggc ttcgatttat tgcgctatct ctgtggcagg
     4981 attgtgaata aagtgtcaac aagcaacggg gcatcgccca ttcaaacatc tataaggaaa
     5041 caccatgagc aaggtattag ttcttaaatc cagcatcctg gcagggtact ctcagtctaa
     5101 tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag cactccgctg atgaaatcac
     5161 cgttcgcgac ctggctgcaa atccgattcc ggtactggat ggcgaactgg ttggtgcact
     5221 gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa gctctggcgc tttccgatga
     5281 gctgattgcc gagctgaaag cccacgacgt tatcgttatt gcggcaccga tgtataactt
     5341 caacatctca actcagttga aaaactattt tgacctggtt gcacgcgcag gcgttacttt
     5401 ccgctatacc gagaacggtc cggaaggtct ggtaacgggt aaaaaagcca tcgttattac
     5461 cagccgcggt gggatccaca aagatggacc aacggacctg gtgacgccgt atctgtccac
     5521 gttcctcggc tttatcggca ttactgatgt gaaatttgtc ttcgccgaag ggatcgcata
     5581 cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa gcagccatcg acagcattgt
     5641 tgctgcataa ccggacacga tcttgatgtg gactgtagtc cagtccacat cccaatccac
     5701 ttacctgttt tcccataatt tcagcatcgc tttgtgatct tccttaagca caatatgcga
     5761 gcgtctggct cgaataaagc taatcgcttc atcaacggtt ttacagtatc cgtaacataa
     5821 cagccatgcc gccactacca gcgcactgcg cgataatccc agtgcgcaat ggaccagaac
     5881 gctgccttgc tcttcgcgta atgtttccag catcgccacg gcctgtcgga gctccccctc
     5941 ttccggaacc accagatcta gcatcggtac acagaaatag agtcgatctt ttgtcgctcg
     6001 tccacgaggg aattcaaagg tgacgtccag aaccgcattc tgtgccggaa tatgtcgtgg
     6061 aaacgcccct aaataaacac cagcagtcat tttgctcacc ggctccaggc gacgacaaaa
     6121 ccaacgcata gatagccaca tcccgatgcg ccagggcaat gtcagccagt aaacggcggg
     6181 cgggagtttc ccctgactat ctttccctgt tgttatcgcg ccaagcccac cgtacccacg
     6241 gccaataatg agtagcgata atactggcca acataaccag actgaccacc ataactgaac
     6301 catcatcatt agctccatca acacaatgca cgcgcacgcg cctacgacat atggcagtgc
     6361 tattttgatt cgacgttgat caggtttctg ataattccac cgacggtcga cgggcaccat
     6421 ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat gatgctgcca
     6481 tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccgcagactt tacgccacct
     6541 cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa tatgcagcga
     6601 aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc atcccgtcac
     6661 cccactcact tcaggacgga taaaactaaa cttcagcgga tagagcaaaa aaccgcagca
     6721 ggccattacc gttgccagaa taagccggtg gacaagtcgg cgctgttcga atgtcgtgct
     6781 acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa caatagtcca
     6841 gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctgggaatat catggctatt
     6901 actgtcctga accgcggtga actgattaag agatccatag gtgaagaaga aaaacggggc
     6961 cagcaacaat aaccagccag ctccttgtag caatacgttc cggcgttcag ttatcacgac
     7021 gcaccgccat cgaaaccgta aaaatgcccc actcatcaat ccgttgtgtg catttatcaa
     7081 atccagcatc atgcacgagc aaatccatct ccccttgcgt acgcacgcgc attacccaca
     7141 gtttaccatc tttatgactg gttaacaccc cggcaatcag ctccagttgt gggtgccacg
     7201 gctgcccggt gtagatgaga atgccacccg gttcgatggc attggcaaga cctgcgagtg
     7261 agttttttac ctgctcattt tcgggaaaca gctcatacag tccagagaca atcgccagcg
     7321 taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcgccc tgttcaaaac
     7381 gcacccgccc agacattccc cgttgggcaa tcatctcttg cccctgtgca acatttaact
     7441 cactgtaatc acgtaataaa atatcgctta cggcaggctc gtttgccagt gcatccagta
     7501 catagcgccc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct ttgacgtgga
     7561 gatcggcaac ggcttgttta atcagtattt gtaaatgagt tttgcgctgg cgaataccgc
     7621 gccagccaac actgttcagg tagattttgt cgattaagcg cccgaatgcg ttactgcctt
     7681 gcggttgatt ttgatagaca taatccagcg aactgcctga atcaaagcca gtgctcattc
     7741 cgagatgcag gcccgcagag tgcgtcccga acaatttcat cgctttacgc ataaagcgat
     7801 acgccaaatc aaccggcgat aatggcacgg gtccacctga aaggaggcgc cagcgatccg
     7861 ctgatggtcc agtgcggtct tcatgctgat aatcaaattt ttgtgacttg ttagcatata
     7921 aacgacgaat aaagctttgc attttttcaa atgcctgcgc cctgttctct tcacccaacg
     7981 tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt aatctctgat
     8041 aaaaatcaat ctgcggttgg cgatgcacca catagtcatc gcctgatatc agaagttgcg
     8101 tggggagcgt aatcgccgcc gcatctctaa taatacgttc agacgtttta tagagatcga
     8161 gcaagatatt gacggcaatc gcccgtgtga tcagcggatc attattgaaa ctcgccaccc
     8221 gttgccgatc gtgggtcaaa tagcgtcttt tcacatagga attaataaaa aacagaccac
     8281 gcagacgatg ccatagcgtc agcgcaggac gtgccagcgg cacatacaat ttaaccttaa
     8341 aggccggaga agccagcacc agcccgcgaa ttgcaggcgc ataatcatga acccatgtgg
     8401 caaccagcac tgcgccgacg ctttgcgcga tcacaaccac ctcttccagt ccaacctggc
     8461 tgtcgctggc agcaaaacgg acaaattcat cgacatcctg cactgaacgc gcaagagatg
     8521 ggctataacc acgcggcccc gaagtttttc catgcccccg ggcatcccat gcataaaaag
     8581 cagtatctgg catcgccagt tcatcaacga tatgttgtag acgaccagaa tgttcatggc
     8641 cacgatgaaa taagacgatg acctttttcg cccctggctg taaagcgggc cagtgccgat
     8701 aaaacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagagatg cgtgaatttt
     8761 ccattttctc tattttcctt cagtaacagc agtaacgaat aaaataaaaa aataccggag
     8821 cggtgaaaat taatgagtca atcctgtcaa ggattccgcc atgtccaggt aatagtttcc
     8881 cgctatcttt aacaccaaca tctcgtttga tggctgacat cactacatcg ccgcaaaaac
     8941 cactaatacc aattaacaaa cccgctaata ttgcctgtaa tgtatttagc ggtgtcagta
     9001 acggtccaat gatcatcgac gctatcatgg tggtgattac gccgccgacc agaccttcca
     9061 gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat ttcccccata
     9121 aatactgtgc aatatcgttt gacgcggtta atgccaaaag aaataacacc agtaaggccc
     9181 cgccctggat attcatggat ggcaacatta ataaccaggc ggcatgactc aaggcgaaaa
     9241 cggtcattat ccagccccaa aaaatggcgc tcacggtatg cagaaaaccg gaggggtcac
     9301 ccacaaaaac tcgccaggtg gctaatatca gaaacccagc caaaggtata aataacagaa
     9361 acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataacc caatataacc
     9421 accgcggaaa atgtacggat ataagcatac aatattcttt caatgccaga aaacttatca
     9481 gcgcaaaaaa cgccaacgtc atccagcgtg gacctgaaat caccagtgaa aaacaaatga
     9541 cgatgcccca ccaggtacga atacgtaatg taacctcacc cccttttctc tccggtaacc
     9601 gccatagcag aaaacggttt atcagcgttg ctaaaattaa caacgcga
//
LOCUS       DAZRUF010000154         9633 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZRUF010000154 DAZRUF010000000
VERSION     DAZRUF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.154"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            85..1026
                     /locus_tag="ACHXOL_002990"
     CDS             85..1026
                     /locus_tag="ACHXOL_002990"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR6809920.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            1014..1952
                     /gene="psuG"
                     /locus_tag="ACHXOL_002991"
     CDS             1014..1952
                     /gene="psuG"
                     /locus_tag="ACHXOL_002991"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR6809921.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            2046..3296
                     /locus_tag="ACHXOL_002992"
     CDS             2046..3296
                     /locus_tag="ACHXOL_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR6809922.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(3363..4061)
                     /locus_tag="ACHXOL_002993"
     CDS             complement(3363..4061)
                     /locus_tag="ACHXOL_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR6809923.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            4191..5132
                     /gene="rihB"
                     /locus_tag="ACHXOL_002994"
     CDS             4191..5132
                     /gene="rihB"
                     /locus_tag="ACHXOL_002994"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR6809924.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5232..6482
                     /locus_tag="ACHXOL_002995"
     CDS             5232..6482
                     /locus_tag="ACHXOL_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR6809925.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(6538..7626)
                     /locus_tag="ACHXOL_002996"
     CDS             complement(6538..7626)
                     /locus_tag="ACHXOL_002996"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR6809926.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(7630..8487)
                     /gene="nfo"
                     /locus_tag="ACHXOL_002997"
     CDS             complement(7630..8487)
                     /gene="nfo"
                     /locus_tag="ACHXOL_002997"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR6809927.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(8561..9610)
                     /locus_tag="ACHXOL_002998"
     CDS             complement(8561..9610)
                     /locus_tag="ACHXOL_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR6809928.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
BASE COUNT         2391 a         2242 c         2480 g         2520 t
ORIGIN      
        1 aacacgtgct ctaatagtct tattgactgt aatggctatt ttacccaatt tcttcgttgg
       61 caatttattg ccagggggcg tgctatgcgc gaaaaggact acatagtaat tattggttcg
      121 gcgaatattg atgtggctgg gtattcacat catccattga attatgcgga ttcgaatccc
      181 ggaaaaatta aatttacccc aggtggcgtg ggaaggaata tcgctcataa tctggcgtta
      241 ctggggaaaa atgcctggct tttaagtgca gtaggtggtg atttttatgg gcaatcatta
      301 ctggcgcaaa ctaatcaatc cggtgtttat gtcgataaat gcttaattgt tccgggtgag
      361 aatacatcca gctatttatc actgctcgat aatacaggag aaatgcttgt cgcgataaat
      421 gatatgagta ttagtgactg tatttctgca gaatttcttg cccagcatca ggagtttatt
      481 cgcggtgcaa aggttattgt cgcggattgc aaccttagtg aagaggcgct ggtctgggtt
      541 ctggaaaata gcggggaaac gcctgtattt attgatccgg tatcggcatg gaaatgcgtc
      601 aaaatccgcg atcatctgag caaaatccat acgttaaaac ctaaccgtct tgaagccgaa
      661 acgttgagcg gaattgcact ttccggacgt gaagatgtcg caaaggtcgc cgcctggttt
      721 catcaacacg gcctgaaccg cttggtgctg agcatgggag gggatggcgt ttactacagt
      781 gatatcaacg gtgaaagtgg gtggtcggcc cctattaaaa cgaatgtcat caatgttact
      841 ggggcaggcg acgccatgat ggcggggctg gcctcctgtt gggtagacgg tatgccgttt
      901 atcgattctg ttcgatttgc gcagggatgt tcgtcaatgg cacttgcctg tgaatatacc
      961 aacaaccctg aattatcaat tgccaatgtt agatcgttag tggagaacac agaatgtctg
     1021 aattaactct gtcccctgaa ttattacaaa tatccgctga agtacaggat gctttaaaaa
     1081 ataaaaaacc ggttgttgcg ctggaatcga ccataatttc tcacggaatg ccattcccac
     1141 aaaatgccca aaccgcaatt gaagtagaag aaacaattcg taaacagggc gcagtaccag
     1201 ccactatcgc cattattggc ggtgtgatga aagtgggctt aagtaaagaa gaaatcgaat
     1261 tgctgggccg tgaaggacat aacgtgacga aagttagtcg tcgtgattta ccttttgtcg
     1321 ttgctgcagg gaaaaatggc gcgactaccg ttgcatcaac catgattatt gcagcactgg
     1381 ctgggattaa agtttttgca accggaggta ttggtggtgt gcatcgcgga gcggaacata
     1441 ccttcgatat ttctgccgat ttacaggaac tggcaaatac caatgtcact gttgtttgtg
     1501 cgggggcaaa atctattctc gatttaggat taacaacaga atatttagaa accttcggtg
     1561 taccgttaat tggttatcag acaaaagcgc tgcctgcgtt tttctgccga accagcccat
     1621 ttgaagtcag cattcgtctt gacagtgccc gcgaaattgc tcgcgcgatg gcagtgaagt
     1681 ggcaaaccgg tttgaacggc ggcctggtgg tagcgaaccc gatcccggaa cagtttgcga
     1741 tgccagaaga aaccatcaat gcagcaattg atcaagccgt tgctgaagcc gaagaacagg
     1801 gtgttattgg gaaagagagt acaccgttcc tgctggcgcg tgttgcggag ctaaccggcg
     1861 gtgatagcct gaaatccaat attcaactgg tctttaataa cgccattctg gcgagcgaaa
     1921 ttgccaaaga atatcagcgt ctcgtgggtt aatttctggc gggcagaaag ccgtcgcccg
     1981 tactcatcaa acataccaaa acctggcgct gacgccgggt ttactggcat gaagggaaaa
     2041 tcattatgga tataatgaga agtgttgcgg ggatggtggt attgctggca attgcctttg
     2101 tgttgtcggt aaataaaaag agcatcagct tgcgcacggt tggcgctgcg ctgctgctgc
     2161 aaattgccat tggtggcatc atgctctatt ttcccccggg aaaatgggca gtagagcagg
     2221 cggcattagg cgtccataaa gtgatgtcct acagtgacgc aggaagtgcc tttatttttg
     2281 gttcgctggt cggtcccaaa atggatgttc tgtttgacgg tgcgggcttt atcttcgcct
     2341 ttcgcgtact cccggcgatt attttcgtta ccgcgcttat cagcttgctg tactacattg
     2401 gcgtaatggg gctgctgatt cgtattcttg gcagtatttt tcagaaagcc ctcaacatca
     2461 gcaaaatcga atcttttgtc gcagtcacca ctattttcct cgggcaaaat gagatcccgg
     2521 cgatcgttaa accttttatt gatcgcatga atcgcaacga gctatttacg gctatttgta
     2581 gcggcatggc gtctattgcg ggttcgatga tgattggcta tgccggaatg ggcgtgccga
     2641 ttgactattt gttagccgct tcgctgatgg ctattcctgg cggtatcctg tttgcacgta
     2701 ttcttagtcc ggcgacggaa ccttcgcaag ttacgtttga aaatctgtcg tttagcgaaa
     2761 caccgccaaa aagcattatt gaagctgcgg cgaacggtgc aatgaccggg ctgaaaatcg
     2821 ccgccggtgt ggcgacggtg gtaatggcat ttgtagcgat cattgcgctg atcaacggca
     2881 ttattggtgg agttggcggc tggtttggtt ttgccaatgt ctctctggaa agcattttcg
     2941 gctatgtgct ggccccgttg gcatggatta tgggggtgga ctggagtgat gcaaatcttg
     3001 ccggaagttt gattgggcag aaactggcga ttaatgagtt tgttgcttat ctcaatttct
     3061 cgccctatct gcaaacgagc ggcacgctgg atgtcaagac cattgccatt atctcgttcg
     3121 ccctctgtgg gtttgcaaac tttggttcta tcggtgttgt cgttggagcg ttttcggcta
     3181 tttcgccaaa acgcgcgccg gaaatcgccc agcttggttt acgggcgctg gcggcggcga
     3241 cgctttccaa cctgatgagc gcgaccattg ccgggttctt tattggttta gcttgagttt
     3301 cattgccgga tgtacgcctt attaagcgtt gcatccggca atcgtgctat tgccaataat
     3361 ttttactgca tcatcccgga gaatttattc tccgggtcca tctccagcgc cagtcctgac
     3421 aactgctttc tgtttttaat tagataccct ttcttgcttt taattaataa accgtcgtga
     3481 atgaattgcg cgagaacata taaaagatgt cgataagaaa cgcctaaata ctctgccgct
     3541 tgcgtatgct tttcgtgata aagatcacct tcctgcgaga gtaatataaa tgctgccagg
     3601 cgattcacta acggaaatga ttgattctga gttaaagaaa caatattacg ataattttta
     3661 tgacttaagg tgacgcagag ttttcgtaaa aatagcgtgt cgtttaataa cagcgggcgg
     3721 taatgtttca tagggagcgc aaggcaccaa cactcttcaa tagcctgcac cgcacgcggt
     3781 tcatggtctt tgtcgattaa ctcaatctcg ccaataaaac agggggcggc aaagaaatcg
     3841 atcagcgaca cgcgaccatt agctagcgtg gcgtaaagcc tggcgcggcc tcgcgtcagg
     3901 taaaacagcc aggacggctg ttgcccttcc tgcacaatgt agtcacgcgc taaaaaatga
     3961 aacagccgcg tatcggctga aacatccgtt aaaaagcaat ccttaaacgc ggattcactc
     4021 atcaattgct gcttaagatc attattgtgg atttctttca cgatgtttat ccgctatgag
     4081 atttctcata tttagcattc gtacgctgtt gttaagattc aatcatcggc ccgatcgaaa
     4141 tttcgttgct gtagaaaaaa taacgcaact ggaaacagag gaaataaaca atggaaaaga
     4201 gaaaaattat tctggattgt gatccgggtc atgatgatgc tattgctata atgatggcgg
     4261 cgaaacatcc ggcgattgat ttattaggta tcaccattgt tgcgggtaat cagacgcttg
     4321 ataaaacatt aattaatggc ctgaatgttt gccagaaact ggagattaat gttccggttt
     4381 atgcggggat gccgcaaccc attatgcgtc aacaaatcgt tgccgataat attcacggtg
     4441 aaaccggact ggatggcccg gtattcgagc cgctgacccg ccaggcagaa agtacccacg
     4501 cggtgaaata catcattgat acccttatgg caagcgatgg cgatatcact ctggtgccgg
     4561 ttggtccgct ttcaaatatc gcggtggcaa tgcgtatgca acccgcaatc ctgcccaaaa
     4621 tccgtgaaat tgtgctaatg ggcggcgctt acggtacagg caacttcacc ccatctgccg
     4681 agttcaatat ctttgccgac ccggaagccg cacgcgtggt gttcacctcc ggcgttccat
     4741 tagtgatgat gggcctcgat cttaccaacc agaccgtttg caccccggac gtaattgcac
     4801 gaatggaaag ggcaggcggc cctgccggag agatgttcag cgacatcatg aacttcactc
     4861 tcaaaacgca gttcgaaaac tacggcctgg ctggcggccc ggtgcacgac gccacctgca
     4921 tcggttatct gattaaccct gatggcatta aaacccagga gatgtacgtc gaagtggacg
     4981 tcaacagtgg cccttgctat gggcgtaccg tctgcgacga gctgggcgtt cttggcaagc
     5041 ccgccaatac taaagtcggc atcactattg ataccgactg gttctgggga ttagtcgaag
     5101 agtgcgtacg cggctacata aaaacccatt aagtgttgat aacaagccgg gacagtatcg
     5161 caagatgcag gcccggctat ttctgatggg cagaaagccg tcgcccaaaa tgacatgaag
     5221 agataatcac tatggatgtc atgagaagtg ttctgggaat ggtggtactg ctggcaattg
     5281 cgtttttact gtcagtaaac aagaagaaga tcagcctgcg taccgttggc gcggcgttag
     5341 tgttacaggt cgtgattggc gggattatgc tttggttacc gccagggcgt tgggtcgcag
     5401 aaaaagtcgc ttttggcgtc cacaaagtga tggcgtacag cgacgcaggt agcgcattta
     5461 tcttcggttc tctggtcgga ccgaaaatgg ataccttatt tgatggcgca ggatttatct
     5521 ttggtttcag ggtattaccg gcaattatct tcgtcactgc actggtgagt attctctact
     5581 acatcggtgt gatggggatc ttaattcgca ttctcggcgg tatcttccag aaagctttaa
     5641 atatcagcaa gatcgagtca ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa
     5701 ttccggcgat cgtcaaaccc tttatcgatc gtctgaatcg caatgaatta tttacagcga
     5761 tttgtagtgg catggcctcg attgctggtt cgacaatgat tggttacgcc gcactgggcg
     5821 tacctgtgga atatttgctg gcggcatcgt taatggcgat ccctggcggg atcttgtttg
     5881 cccgcctgtt aagcccggct acggaatctt cacaggtttc ttttaataac ctctctttca
     5941 ccgaaacacc gccaaaaagc attattgaag ccgctgcgac aggggcaatg accgggctga
     6001 aaatcgccgc cggtgtagcg acagttgtta tggcatttgt tgcaataatt gcgttgatta
     6061 acggtattat cggcggtgtt ggcggctggt ttggttttga acatgcttcg ctggagtcga
     6121 ttttaggtta cctgttggcc ccattggcgt gggtgatggg ggttgactgg agtgatgcaa
     6181 atcttgccgg gagtttgatt gggcagaagc tggcgatcaa tgaatttgtc gcttatctca
     6241 atttctcgcc atatctgcaa acgggtggca ctctggatgc taaaaccgtg gcgattattt
     6301 ctttcgcgtt gtgcggtttc gctaactttg gttctatcgg ggtggtggtg ggggcgtttt
     6361 ctgcggttgc gccacaccgt gcgccggaaa tcgcccaact tggtttacgc gcgctggcgg
     6421 cggcgacact ttctaacctg atgagtgcta ctattgcagg attctttatt ggtttagcgt
     6481 agtgcgcaat ttatgagccg gataagatgc ggtcaagcgt cgaatccggc aacattgtta
     6541 caccattggc actaatgaaa gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc
     6601 gctggcgcgc gaaattgccg cgcaggctac cgcaaaacgg gcgctgtcgc ggaaactgta
     6661 tccttcaaga aagctatata ccaggcccgc cataaaacca tcgtcagcac caaaactgtc
     6721 tactgtcgta tgcgctggcg cggtcagcaa aaattgttct ccatccttat cgctgcaaaa
     6781 tacagattca tctttcagat aaacaaaaat gcgtttaaca ccttgttgat gcagcgaatt
     6841 tacagctctg atgcgatcgt tgtcgtcttt aatcggttgc ccccacaaaa tttccagttc
     6901 attttgtgtt ggtttcagag tgtggatccg gctgtaccag ttttttactt tgttcgcttt
     6961 aaattcggaa acggtatcga caaacatcgg aatttcatca gcaatggtaa agacccactc
     7021 cagggcttca ggtgtgagat tacaatcagc cagcaccaca ccagcatggc gaatcaagtc
     7081 ccgtgaagta ttgagcagtt gaggcgttag ctgttgcaga atatgtgtgt cattgattgc
     7141 caggatagtt tcttcctgct tattagcgat agcgagataa gttgcagtgc tgtgtccatg
     7201 cagacgaatg cagtttgaaa cgttcacgcc cgcgcggcgc gtttcttcca gtaatgtctc
     7261 gccgtaaaaa tcgttgccaa tagctgatat taaatgcaca tcccgaccaa gcagagccag
     7321 gttatgcgcg atattacgtc cgacgccgcc tgcggaacag tgaacagaac ctggatgaga
     7381 ggctgcttgc gggtaacgga tatccgccat tccgcgaata tccatattga ttgcacccac
     7441 aaccacacag taatcctgct cggtaaggat atatcctttg cctttaatca aacccttgcg
     7501 catcaaatcc ataatatgag cggcaacacg tgagcggctg atttgcagaa tatcggcgat
     7561 ttcattctgt tgaatcagcg ggttacgccg taaaatttta agaatctgct tttccctgtc
     7621 gttcatcgat caggccaccg ctttttcagt ttgttgcgct ttcagccagg cgatctcttc
     7681 tgcccagata tccgggttga tggtttcaag gatcagcgga atgccgtcga aacggtcgtc
     7741 ttgcatgatc cagcggaacg catcatgacc gatattgcct tcaccgaggc tatgatggcg
     7801 gtcaacgcgg ctgccaaagg tacttttcgc atcgttaagg tgcatcccgc gcagatactt
     7861 aaagccgaca atacgggcaa aatcagcgaa cgttttttcg cattcggctg gagtacgcaa
     7921 atcatacccg gcagcgaaag catggcaggt atcaatgcag acgccgacgc gggatttatc
     7981 ttccacgccg tcgataatcg ccgcgagatg ttcgaattta aaccctaagt tactgccctg
     8041 accggcggtg ttttcaatca ccgctgttac accttgagtt ttatccagcg caatgttgat
     8101 ggattcggca atacgcgcaa ggcaatcctc ttctgaaatc tgcatcagat ggctgccagg
     8161 gtggaagttg agcaaagaaa gccccagttg ttcgcaacgc tgcatttcat ctataaaggc
     8221 atcgcgcgat ttttccagag cttcagtgac cggatgtccg aggttaatca gataactgtc
     8281 gtggggaaga atttgcgccg atgtgtagtg atatttttca caggcggctt tgaattcatc
     8341 gatggtttgc gtcgtgagcg gtgcggcacg ccactgacgt tggtttttgg tgaacaaggc
     8401 aaacgcggtt gcgtcgattt cggcggcgcg aattgcggca tttgccagac cgccagcagc
     8461 actaacgtgc gctccaatgt atttcatgcg aggactcctg ttaaacccgc tggaggaaaa
     8521 cggtaatgat agcgggttaa caggagcaag atgtagtggt ttatgcgatg acgctttgaa
     8581 tcacatagtt aatcgcacca ccaccaacaa tcagccaggc aaacagtacc agtgccatca
     8641 gcagaggttt cgccccagct tttttcagcg cgctgacgtg agtggtcaga cccagcgccg
     8701 ccatcgccat tgccagcagg aaggtatcca gcgttaccag catgttcacc acgctctgcg
     8761 gtaacaggtg gaacgagtta aagatagcaa cgacgatgaa caagatggca aaccacggaa
     8821 tagtgatttt gcttttctcg ccgctgttcg ccccagacag ctgtttaaca cgcgccgcca
     8881 gcaggatgag gaacggagcc agcatcatca cgcgcagcat tttggaaata actgctgcgt
     8941 tttccgcatc cgggctgatg gcatgacctg ccgccaccac ctgcgccact tcgtgcacag
     9001 tagaaccaat gtagataccg aaagtttccg gactaaacca ttgagacatc agcggatata
     9061 tcgccgggta gaggaaaatc gcgacggtcc cgaagataac aacggttgca acagccacgg
     9121 ttactttact ggcttccgct ttcactaccg actcagtcgc cagtaccgcg gcagcaccac
     9181 agatactgct accggcaccg atcaaccagc tggtgtgctt atccagacca aacactttct
     9241 gccccaggaa gcaagccagc aggaaggtac tggacagcgt caacacgtca atgatgatcc
     9301 cactgatacc gacatcggca atttgcgaga acgtcagacg gaagccataa agaatgatac
     9361 ccagacgtaa taaatattgc ttggcaaaca gcacaccacc gtcacagctt ttccagatgt
     9421 gcggatagat ggtgttgcct aaaaccatcc ccaacaagat tgcgagggtg agggcactaa
     9481 acccggcacc cgcaaccgcg ggaatggaac caccccacag ggcgaccccg gtgataactg
     9541 cactcagggc taaccccgga ataaaatgcc acagtgtacg atgttgtttc tgtaaggtga
     9601 tattcgtcat aaccctctcc tttaaccggg cat
//
LOCUS       DAZRUF010000155         9621 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZRUF010000155 DAZRUF010000000
VERSION     DAZRUF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.155"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(1..414)
                     /locus_tag="ACHXOL_002999"
                     /pseudo
     CDS             complement(1..414)
                     /locus_tag="ACHXOL_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(520..3081)
                     /gene="mutS"
                     /locus_tag="ACHXOL_003000"
     CDS             complement(520..3081)
                     /gene="mutS"
                     /locus_tag="ACHXOL_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR6809929.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3157..3291
                     /locus_tag="ACHXOL_003001"
     CDS             3157..3291
                     /locus_tag="ACHXOL_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809930.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            3368..3721
                     /locus_tag="ACHXOL_003002"
     CDS             3368..3721
                     /locus_tag="ACHXOL_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR6809931.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(3758..5835)
                     /gene="flhA"
                     /locus_tag="ACHXOL_003003"
                     /pseudo
     CDS             complement(3758..5835)
                     /gene="flhA"
                     /locus_tag="ACHXOL_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(5909..6919)
                     /gene="hypE"
                     /locus_tag="ACHXOL_003004"
     CDS             complement(5909..6919)
                     /gene="hypE"
                     /locus_tag="ACHXOL_003004"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR6809932.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(6916..8037)
                     /gene="hypD"
                     /locus_tag="ACHXOL_003005"
     CDS             complement(6916..8037)
                     /gene="hypD"
                     /locus_tag="ACHXOL_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR6809933.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(8037..8309)
                     /gene="hypC"
                     /locus_tag="ACHXOL_003006"
     CDS             complement(8037..8309)
                     /gene="hypC"
                     /locus_tag="ACHXOL_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR6809934.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(8300..9172)
                     /gene="hypB"
                     /locus_tag="ACHXOL_003007"
     CDS             complement(8300..9172)
                     /gene="hypB"
                     /locus_tag="ACHXOL_003007"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR6809935.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(9176..9526)
                     /gene="hypA"
                     /locus_tag="ACHXOL_003008"
     CDS             complement(9176..9526)
                     /gene="hypA"
                     /locus_tag="ACHXOL_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR6809936.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
BASE COUNT         2167 a         2618 c         2534 g         2302 t
ORIGIN      
        1 gatggcatat tttatgttgt cattaatgat ttcaataata tgtggaaggt gatggaattt
       61 cagcaacaga tctattgcct cttgttgctc tgaatcattt aaatcgaaaa accagtcacc
      121 accgctggca agccacatat ttccatcgcc agtttcgaat gcatccaacg ccatcgcttc
      181 gtggttgcct ttaaccgacg taaaccaggg ttggtttagc aggcgcagca cgttaagact
      241 cttcggccca cgatcaatat tatcgcctgt ggaaataagt aagtcggttt cagggtaaaa
      301 agagagttga tgttaacggg attgtaataa ctgatattca ccatgaatat caccaacgac
      361 ccatatatgg cgatagtgat gggcattgat tttttgatag cgtgtagatg gcatggtttt
      421 accctgtaaa ataagctttc ctattataca gggtattttt atttgattcg tcagttgtcg
      481 ttaatattcc cgatagcaaa agactatcgg gaattgttat tacaccagac tcttcaagcg
      541 ataaatccac tccagcgcct gacgcggagt cagtgaatcc gggtcgagat tttccagtgc
      601 ttcgaccgca ggcgaagttt cttctggcac tgacagcaaa gacatctgcg taccatccac
      661 ttgcgtcgcg gcggcgttcg gcgaaatgct ttccagctca cgcagttttt gccgtgcgcg
      721 cttaataacc tctttcggta cgcccgccag agcagcaacc gccagaccgt agcttttgct
      781 cgccgcgccg tcctgcacgc tgtgcataaa ggcgatagtg tcgccgtgct ccagcgcatc
      841 gagatgtacg ttggcgacgc cttccatttt ctccggtagc tgggtcagct cgaaatagtg
      901 ggtggcaaac agcgtcaatg ccttaatctt attcgccaga ttttctgcgc acgcccacgc
      961 cagcgacaga ccatcgtagg tggacgttcc acgcccgatc tcatccatta acaccagact
     1021 gtattcggtg gcgttatgta aaatattggc ggtttcagtc atctccacca taaaggttga
     1081 gcgcccggaa gccaggtcat ctgccgcgcc tacgcgggta aagatgcgat cgataggtcc
     1141 aatctcgact ttttgtgccg gtacatagct gccgatgtag gccatcagcg caatcagtgc
     1201 ggtctggcgc atataggtac ttttaccgcc catgttcgga ccggtgatga tcaacatacg
     1261 gcgctgcggc gacagattca gcgggttagc gataaatggc tcattcagta cttgttcaac
     1321 taccggatgg cgaccttcgg taatgcgaat gcccggttta tcaatgaagg tcgggcaggt
     1381 gtagttcagg gtataggccc gttccgccag gttcaccagc acgtcgagtt ccgccagcgc
     1441 gctcgcgctc tgttgcaacg cttccagatg cggcaacagc aggtcgaaca actcttcata
     1501 aagctgtttt tccagtgcca gtgctttgcc ttttgaggtg agaactttat cttcgtactc
     1561 ttttagctct ggaatgatgt agcgctcggc gtttttcagc gtctggcgac gcatgtagtt
     1621 gatgggtgcc agatggcttt gcccacggct gatttgaatg tagtagccgt gcaccgcatt
     1681 aaagccaact ttcagcgtgt ccaggccggt acgttcacgc tcgcggactt ccagacgctc
     1741 cagataatcg gtcgcgccgt cagccagcgc gcgccactca tccagctctt cgttatagcc
     1801 cgatgcgata acaccaccgt cgcgtaccag caccggcggt gtgtcgatga ttgctcgctc
     1861 cagcagatcg cgcagctcgg caaactcgcc catcttctca cgtagcgcct gtaccggtgc
     1921 actatcgaca gtttctaact gcgcacgcag ctccggcagt tgctggaaag cgtggcgcat
     1981 acgggccaga tcgcgtgggc gagcagttcg taaagccaga cgtgccagaa tacgttccag
     2041 gtcgccgacc tgacgcagta ccggctgcaa ctcggcggtg aaatcctgca atgcgccaat
     2101 agtttgctgg cgctcaagca acacgcgggt atcgcgcact ggcatatgca gccagcgttt
     2161 cagcatacga ctacccatcg gcgtgacggt gcagtcgagc acagaagcca gcgtattttc
     2221 cgcaccgccc gccaggttct gagtaatttc caggttacga cgtgtcgcgg catccataat
     2281 gatgctgtcc tgctcacgtt ccatggtgat agaacgaata tgcggcaggg tcgtgcgttg
     2341 ggtatctttc gcatactgca acagacaacc ggcagcacaa agtccgcgcg gcgcgttctc
     2401 gacgccaaaa ccgaccagat cgcgggtgcc aaattgcaga ttcaactgct ggcgcgcggt
     2461 gtcgatttca aactcccaca gcgggcgacg gcgcaggccg cgacggcctt caatcagcga
     2521 catctcggcg aaatcttctg catacagcag ttccgccgga ttagtgcgtt gcagctctgc
     2581 cgccatcgtt tcgcggtcgg ccggttcgct caggcgaaaa cgcccggagc tgatatccag
     2641 cgtcgcgtag ccgaaacctt tgctgtcctg ccagatagcc gccagcaggt tgtcctgacg
     2701 ctcctgtaac agggcttcat cgctgatggt gcctggcgta acgatacgca caactttgcg
     2761 ctcaaccgga cctttgctgg tcgccggatc gccaatttgt tcgcagatgg caacggactc
     2821 tccctgattc accagtttgg cgagatagtt ttccaccgca tggtagggaa tccccgccat
     2881 cgggatcggc tctcccgccg aagcaccgcg tttggtcagt gaaatatcca gcagttgcga
     2941 cgcgcgtttt gcgtcgtcat aaaacagttc ataaaaatca cccatccggt aaaacagcag
     3001 gatctcggga tgctgggctt tcagcctgag atactgctgc atcatgggcg tatgggcgtc
     3061 gaaattttct attgcactca tggggttatg tccggttccc tgatattaaa tggggtgtga
     3121 tggtttttat tttcgttgta atcacataag gcgcacatga taccggagtt aatcatttca
     3181 ggggaagaag cgaacggcga gttcttgatg ctggttccgg gaaagatgct gttttgcagc
     3241 ttttgcgtga tgcttatgaa gcaagtacgc aaaattgcgt cggttgaata aacctcctcc
     3301 ttcagagcca tagtcccctt tcaaattaag ttgtaatata aatacacctt aagagtatgg
     3361 aggcagcatg tccggcaagc gtatctctcg tgaaaaactg acgattaaaa aaatgatcga
     3421 tctttatcaa gcgaaatgcc cgcaggcgtc agcggagccg gagcattacg aggcgttgtt
     3481 tgtttacgcg caaaagcggc tggataaatg tgtgttcggc gaagagaaac cagcctgtaa
     3541 gcagtgtccg gtgcactgtt accagcctgc aaagcgtgaa gagatgaaac agattatgcg
     3601 ttgggccggg ccgagaatgc tgtggcgtca tccgatctta accgtgcgtc atctcatcga
     3661 tgacaagcgt ccggtaccag aactgccgga aaaatatcgt ccgaaaaaac ctcgtgaata
     3721 gtatgccgga caggcagatc tgtccggcaa tttgcagtta aatcaatgcc gatttatcaa
     3781 ttcccagccg cttcatccgt gacagcaggg tcgtgcgttt cagccccaga cgttgcgcag
     3841 cgcctttagg cccggcaacc acgccgttgg tttctttcag cacgcgcaca atcaactgat
     3901 attcatcttc gccctcctgg gcgacaaccg ttgcggcagg cggcgtttca ggttccggta
     3961 aaacaatatc tggcaatgac agctgcagca cgttaccgcg tgttagcaat accgcgcgct
     4021 caatgacgtt ttccagttcg cgtacgttac ccggccactc catgttgctc aaggtgcgca
     4081 gcgtctcggc aggaatgctg tcgatattgc gccccagacg acgggcaatt ttgaaggtaa
     4141 aggctttcgc cagcagcgga atatcttccg gacgctcgcg tagtggcggc aggtgaatcg
     4201 ggaatacgtt caggcggtaa tagagatcgc tatggaactc tcggtcggcg accatttttt
     4261 tcagatcgcg gttagtcgcg gcaattaaac gtacgtccgt ctgaatgatt ttgttgctgc
     4321 cgagacgttc aaactcctgt tcctgcaata cacgcagcaa cttcggctgt aactccagtg
     4381 gcatatcgcc cacttcgtcg aggaacagag agcttttatc cgccagttca aaacgaccaa
     4441 tgcgctgggc gctggcaccg gtaaaagccc cacgctcatg accaaacaga tcgctttcca
     4501 gcaatccggc aggcatcgcc gcgcagttca ttttgaccat gcggcgatta ttacgcccac
     4561 tgagattatg gatcgcgcgg gcaatcagct ctttacccgt gccagtttca ccgaggatca
     4621 gcacggtact gtcactttgc gccaccattt caacttgttt aagcacgctg tacatggctt
     4681 cgctgcggcc aataatctcg ccaaattcac tatcaacatt gttgagctgc tcggtcaggg
     4741 cgaggttttc atcaaccagc cgttctttca gacgatggat ttcctgatag gcgagggcgt
     4801 tatcgacagc gattgccaca cgttcggcaa tctggcgcag caaattcaga ttggtagtgg
     4861 taaacacttt ctcttcgcat tgcgccagtt tcagcacgcc cagcatggtg tcgccagaca
     4921 tcagcggtaa caggcacaag gtttgaatct ggttgcccta ggtgtcgaac aacatgcgtt
     4981 catagggggc taaatcgtcc cgctcgtgga gattgatcag cagcatctct ttacttttga
     5041 acacgcgttc ggtgagggtt ccggcttcat cgacttcgct ctgttcgtgg gcgggatgtt
     5101 gtttatcaag atagtgagtg gagtagatgt tgagtttgtt tttacggtgg ctgcgtaaga
     5161 cgatactgat atcgtcaatg tcgaaatagt aatggatttc tttggcgact tcgctgacca
     5221 gttcgtccat atccaggcgg gaaagcaccg cgttggtaat ggcaaccagg atgcggaagt
     5281 tatcgcgctc ccggcataac aactcatagt cgacattgtt gacgacgcgg ttctggattt
     5341 gttcggtgac gacagaaacg atctgcgtaa atgtttgcag acgattgaac tctttttcgc
     5401 tccagggacg atcgtcataa cgaataaatt cacagccacc aaaaatatgc ccttccgccg
     5461 ccagtggcat caggcaatag tgaccaaatt tggggtatag cccacctgcg accagctgcg
     5521 gccaggtttc acaaaattct tcgtaactgc aatgcagcgt atcagggcgc gacaaaatgc
     5581 ggcgtaccgg accgtgtgcc agaacagttt cgtcttcata tttaatgggg tgtctttttc
     5641 acgcgatgcg taataagacg cacgttgagt ctgcgcttgc cacaacacaa tagccgcgtt
     5701 gtcggcgagc gcagaacgct ttaccagttg cgaaagagcc tcacacagcg aggccagatc
     5761 gggctgctgc aatagtgtcc gagtgatgtc gaacaaccct tgctgtccga gatcactcat
     5821 cggtgtatat gacattttgt tcttccagga aagcaccgca aagcggtgca aaaggtcatt
     5881 gactaactat aggagattta gaattttatt agcatatacg cggaagaggt tcggcgtgtg
     5941 gtaaatcgag ggttcgtttc acaccataca gaccggcaag acgaacacct ttacgttcca
     6001 ccacttcacc aattagcgcc gcgtctttcc ccagtggatg ggaatgtaac gctgccagca
     6061 cttgctctgc cgcgttgcgt tcaacggcga tcaccagttt gccttcgttg gcaaagttaa
     6121 gggcgtccag tcccagcagt tcgcaaacgc cgcgcacggc aggtttaacc ggcagtgcca
     6181 cttctgaaat ttcaatacca caaccgcagg ctgccgcgaa ctcatgaact accgcgttca
     6241 caccaccacg ggtggcatca cgcagcgctt tcacgccggg aatgtcacgc agcgtctgaa
     6301 taagcggcgt cagtaccgcg cagtcgctga ccagttcgcc atccagcccc agctgctcac
     6361 gcaggttaag gatagtcgcc ccgtggtccc cgagcgtgcc gctaaccagt aatacatcgc
     6421 ctgcggttag cgtctgtgcg ccccagtgaa tattcgccgg aattgcgccc atgccagcgg
     6481 tgttgataaa cagtttatct gccgcgccgc gctgcaccac tttagtatcg ccagtaacga
     6541 tggcaatgcc tgccgcgcgc gcggtttctg ccatgctggt cactacggct ttcagtgtct
     6601 ccatcggcaa tccttcttcg aggataaagc cacaggagag atagcgcgga atagcgccac
     6661 tgaccgcaac gtcattggct gtgccgcaaa tcgccagctt gccgatatta ccgccaggaa
     6721 agaacagcgg gtcaatgacg taactgtcgg tggagaacgc cagacggtcg ccttctgcta
     6781 ccagctgcgc cagatcaaga cgtgcctggt cttcctgctc tgccagccac gggttggcaa
     6841 aggcttccat aaacaggctg ttgattaatt gctgcatcgc ctggccgccg ctgccgtggg
     6901 cgagttggat attattcacg cttcattctc ctgctgacga tactgatacc acgcggcgca
     6961 cgctccttcg gaggagacca tcagtgcacc aaatgcggtt tgaggattac aggtgttacc
     7021 aaacagcggg cattgatgcg gcttacattt gcccgttaat acctcaccac aacgcgcgcg
     7081 cgggtcatcg cagacctgct gcggtgctgg gcggaaatgt gcttcggcat cgaatcgttg
     7141 ataatccggc gtcaggtgca cgccagaaga ttcaatcacg cctaagccgc gccattcgct
     7201 gtcgccgttg acacagaaca catcagcaat tgcctgttgc gccagcaggt taccggcatc
     7261 cggtaccacc cgacgatact gattctctac cttgctgtgg gccgctattt tctgctccac
     7321 cagcatgacc acgccttgca gtagatcaag gggttcgaaa ccagcgacca ccagcggacg
     7381 atgaaaatcg ctggcgataa aattataggc atcagtgccg ataaccatac taacgtggcc
     7441 cggcgcgagg aacgcgtcga taccgttatc cggctgttcc agcaaactgc gcagcgtcgg
     7501 gataagcgta atatgctggc agaagaagta aaaattctgc acatcgcggg ctttcgcctg
     7561 ttgcagggtg atggcggtgg tcggcatggt ggtttcaaaa cctaagccga agaatacgac
     7621 tttacgggtt ggattctcct gcgccagttt caacgcatcc atcggcgagt aaacgatgcg
     7681 cacatcggca ccgcgtgctt ttgcctgcaa cagcgatccc tgtttccccg gcacgcgcat
     7741 ggcgtcgcca aaggtacaga agatgacttc cggatggctg gcaatctcca cgcaggtgtc
     7801 gattctaccc atcggcagta cgcacaccgg gcaacccgga ccgtggataa actcaacgtt
     7861 ttcaggcagt aactggtcga ggccgaattt aaaaatggcg tgggtatgac caccgcacac
     7921 ttccataatc cgcagagggc gttcggcggt gtaagagaga tgtgaagcac gttcgcgcag
     7981 atgctcaatt aactgcatca cctgttccgg cgcgcgatat tcatcaacaa aacgcattat
     8041 ttttcctcgc catacaacag cgcgccgaca tccggctcaa cgtcaaacat gttttgtaag
     8101 gcgtcgagag tgtcgcgtgc ttcggcttca ttaattacgc tcatggcaaa gccaacgtgt
     8161 accagtaccc actggcccac gcgcggctga ccgttttcat cgcagctgcc gactaacgtt
     8221 aaatcgacat cgcgctgaat gccgcagacg tcgacttttg cctggttgcc gtcaatggtg
     8281 cggatctggc cgggaacgcc tatgcacatc gctgtgtctc cagccagttc agccactggt
     8341 ccatcccttc gccgctggtg gcggaaataa ggatgatttc aatttctgga ttgacttcgc
     8401 gggcgcaggc gatgcacttc tcaacgtcaa agttgagata cggcaacagg tcaactttgt
     8461 tgagcagcat cagcgaggcg gcggcaaaca tatgcggata tttcagcggt ttgtcttcac
     8521 cttcggtaac ggaaagcacc gccactttgt gtttttcacc gagatcgaag ctggccgggc
     8581 atacgaggtt gccaacgttt tcgataaaca gaataccgtt atcgtccagt ggcagacgcg
     8641 gtgcggcgtc ggcaatcatc tgtgcgtcaa gatggcagcc tttaccggtg ttcacctgaa
     8701 tcgctggtgt gccggtagcg cgaatgcgtg cggcatcgtt cacggtttgc tggtcacctt
     8761 caataaccgc gcacggaacg ctgtctttca ggcgcattag ggtttccgtc agcagggtgg
     8821 ttttaccgga accagggctg gaaaccaggt tgagcaccag ttgcttgcgg gcagcaaagc
     8881 gcgcgcggtt gcgttcagcc agacggttat ttttgtccag cacgtcaatt tcgacttcca
     8941 gcatccgacg ctggctcatg cccggcgcgt gagtgcccgc ttcgccatga ccgtaatgca
     9001 ggtcgccttc ttcagtctgg ctgggggtaa attcaggcgc tttaatgccg gtgattttca
     9061 tcttcgggcg tgccgccggg gcaaatggcg cgctacgaaa cgcggaatga gggttatgtt
     9121 catcaccctc gatatacagg ttgccttcac cgcaaccgca tgttgtacac atcgctcact
     9181 cctggtctat ttctatccgc cgaatctgta aaccgtcgtc tgccacaatc tgtagcatgt
     9241 caccatgaca ctgtggacag cggcggacgc gctgggtcag tagcgtcaca tactgttggc
     9301 atgtttcaca ccagcattcg gcctcttgtt cttcgaggtg cagtttacaa ccttccgcca
     9361 cgctgccgcg gcaaaccaga tcaaaacaaa aggcaagaga gctggtttcg acacaagaaa
     9421 atgcgccaat tttgagccag accccagtta cgcgttttgc gccgtgtttt gcggcctgct
     9481 gttcgatcaa ttccagtgcc cgttggcaga gggttatttc gtgcatatcg cctcccatta
     9541 actattgcca gctacaagca ataattgtgc cagtgttgat tatccctgcg gtgaataatg
     9601 tcgatgatgt cgaaatgaca c
//
LOCUS       DAZRUF010000156         9614 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZRUF010000156 DAZRUF010000000
VERSION     DAZRUF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.156"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHXOL_003009"
     CDS             <1..173
                     /locus_tag="ACHXOL_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR6809937.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="ACHXOL_003010"
     CDS             complement(345..674)
                     /locus_tag="ACHXOL_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR6809938.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="ACHXOL_003011"
     CDS             complement(679..864)
                     /locus_tag="ACHXOL_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR6809939.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="ACHXOL_003012"
     CDS             complement(861..3500)
                     /locus_tag="ACHXOL_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR6809940.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVKPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHXOL_003013"
     CDS             3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHXOL_003013"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR6809941.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHXOL_003014"
     CDS             4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHXOL_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR6809942.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5227..5493)
                     /locus_tag="ACHXOL_003015"
     CDS             complement(5227..5493)
                     /locus_tag="ACHXOL_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR6809943.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHXOL_003016"
     CDS             5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHXOL_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR6809944.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2404 a         2493 c         2332 g         2385 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg cttgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttccc catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 gttgttgatg aatttttcaa tatcgccata gctttcaatt atatttgaaa ttttgtaaaa
     3661 tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg
     3721 tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct
     3781 ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact gccaatggct
     3841 gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg gaagagctga
     3901 aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg
     3961 acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaggccg
     4021 ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc
     4081 agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact atgtatggca
     4141 aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc attctgaaag
     4201 gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg
     4261 gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact
     4321 gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat cagatgaaaa
     4381 atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcagcaattg
     4441 aagcgctgaa aaatcagaaa attgggtcgt tgggtatgga cgtctatgag aacgaacgcg
     4501 atctattctt tgaagataaa tccaacgacg tgatccagga tgacgtattc cgtcgcctgt
     4561 ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca gaagctctga
     4621 ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc
     4681 cgaacgaact ggtttaatct tgccgctccc ctgcattcca ggggagctta ttcagataat
     4741 ccccaaagac ctttcatctt ctattcttaa aatagccctg agtcagaaac tgtaattgag
     4801 aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt
     4861 aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct ttgtgctgga
     4921 aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga
     4981 aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa
     5041 tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct
     5101 taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac
     5161 cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt
     5221 aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc
     5281 gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa cagaaagcga
     5341 accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacctgg
     5401 cgctacgtgc gccgcagtcg cctgctgaac aggttcacta ctacacgccg acaacaataa
     5461 agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc ttcaagccaa
     5521 acgacaccgc cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt
     5581 tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc
     5641 attacgctga tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt
     5701 gaaggattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg
     5761 tgtcatatga ttactattga tggtaatggc gcggttgctt cggtggcgtt tcgcaccagt
     5821 gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc
     5881 tgggccggaa acggcttgaa gaacgtttgg ggagacacac catgcgtggt tgaaatgcag
     5941 tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctttcaaca
     6001 tcgtttacgt catcgcaggg tttgctgctg atgatcccga cactgtacaa actggcaggc
     6061 gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactctct
     6121 atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt
     6181 gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc gacgctgaaa
     6241 agccgcgtgc catttattca tttctttgat ggtttccgca cgtcccacga aatcaacaaa
     6301 attgtcccgc tggccgatga cacgattctt gatctcatgc cgcaggccga aattgatgct
     6361 catcgcgccc gggcgctcaa cccggaacat ccggtgatcc gtggtacgtc cgccaatcct
     6421 gacacttatt tccagtctcg cgaagccacc aacccctggt acaacgcagt ctatgaccat
     6481 gttgaacagg cgatgaatga tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa
     6541 tattacgggc atccgcaagc tgaacgggtg attatcctga tgggctcagc cattggcacc
     6601 tgtgaagaag tggttgatga attgctaacc cgtggcgaaa aagtcggcgt gctgaaagtt
     6661 cgcctgtacc gccccttctc agccaaacac ttgctacaaa ctctaccggg atccgtacgc
     6721 agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc gctctatctg
     6781 gatgtgatga ccgcactggc agaagccttt aataatggca agcgcgaaac tctgccccgt
     6841 gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg
     6901 gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg tatttacgat
     6961 gatgtaacca atctgtccct gccgttgccg gaaaacaccc agccaaactc ggcgaaactt
     7021 gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc
     7081 aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa
     7141 aaagcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat tcgttccgct
     7201 tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat
     7261 cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc gtacagcgca
     7321 gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca gaaaaaagcg
     7381 cgcttctatg tgattaacgc ggcgaaaatc gcccgcaaat gtggcctggc ggcccgtatt
     7441 aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc
     7501 ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca ggatctggtg
     7561 gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa
     7621 ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc ccctgatttc
     7681 gtgaaaaccg taaccgcagc gatgctcgca gggcttggtg atgccctccc cgtttcggcg
     7741 ctgccgccag acggcacctg gccgatgggc accactcgct gggaaaaacg caatatcgcc
     7801 gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgcagct
     7861 tgcccacact cagctattcg cgcaaaagtg gtaccacccg aagcgatgga aaacgcccct
     7921 gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg
     7981 caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa
     8041 gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga acatgtcgaa
     8101 gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg tagcaaactg
     8161 gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata ttccggtgct
     8221 tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta tggcgaccgg
     8281 atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca
     8341 ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct atttgaagat
     8401 aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg
     8461 cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc gttgaaatca
     8521 gacgctacgc cagaggttcg tcgagaacag gtcgcagctt tacgccagca actcaacgat
     8581 gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa atcaatctgg
     8641 ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg
     8701 agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt
     8761 ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga gcacggcaaa
     8821 cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg
     8881 cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg
     8941 tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg
     9001 gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc gctatatcgc
     9061 ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg
     9121 tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct gaattcgcag
     9181 cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat
     9241 gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg
     9301 ataaggatta cccaattcta aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     9361 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     9421 gcatataacc cgcgcagaat aacgtataga aaattaaaag tataattttt attttgtatg
     9481 tattatcttc gatacattca ccagacttat tatcatttca gaagaattat ttgattaagg
     9541 ttttacttaa ggcgtaacaa atgatattta tcggctaact gaacttctcc tttaggatgt
     9601 tttcactccc cgga
//
LOCUS       DAZRUF010000157         9611 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZRUF010000157 DAZRUF010000000
VERSION     DAZRUF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.157"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..2199
                     /locus_tag="ACHXOL_003017"
                     /pseudo
     CDS             1..2199
                     /locus_tag="ACHXOL_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2192..3328
                     /locus_tag="ACHXOL_003018"
     CDS             2192..3328
                     /locus_tag="ACHXOL_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR6809945.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="ACHXOL_003019"
     CDS             3325..5325
                     /locus_tag="ACHXOL_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR6809946.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5450..5911
                     /locus_tag="ACHXOL_003020"
     CDS             5450..5911
                     /locus_tag="ACHXOL_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR6809947.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5952..6422)
                     /locus_tag="ACHXOL_003021"
     CDS             complement(5952..6422)
                     /locus_tag="ACHXOL_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR6809948.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6469..7188)
                     /gene="btsR"
                     /locus_tag="ACHXOL_003022"
                     /gene_synonym="yehT"
     CDS             complement(6469..7188)
                     /gene="btsR"
                     /locus_tag="ACHXOL_003022"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR6809949.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7185..8870)
                     /gene="btsS"
                     /locus_tag="ACHXOL_003023"
     CDS             complement(7185..8870)
                     /gene="btsS"
                     /locus_tag="ACHXOL_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR6809950.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9092..9157
                     /locus_tag="ACHXOL_003024"
                     /pseudo
     CDS             9092..9157
                     /locus_tag="ACHXOL_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9385..>9611
                     /locus_tag="ACHXOL_003025"
     CDS             9385..>9611
                     /locus_tag="ACHXOL_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR6809951.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESA"
BASE COUNT         2352 a         2530 c         2463 g         2266 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgctttttac
     5341 taagagttct actccagttc cggactgctc acgccacggt attaggcata tcctatatag
     5401 cccctggtga gagtcaccag ttccttgatt aaataaaatg gagttttaca tgaaggcttt
     5461 caataagctg ttttccctcg ttgttgcatc tgttctggtt ttctctcttg ctggctgcgg
     5521 tgacaaagaa gaatcgaaga aattcagcgc caatctgaac ggcactgaaa ttgccattac
     5581 ctatgtctac aaaggtgaca aggtgcttaa gcaatcttct gaaaccaaaa ttcaatttgc
     5641 ctccattggt gcaaccacca aagaagatgc tgccaagaca cttgagccgt taagcgccaa
     5701 atacaaaaac atcgcgggtg ttgaagaaaa attaacctat accgatacct acgcgcagga
     5761 aaacgtgact atcgatatgg aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat
     5821 caacgtttct gctgaagatg ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat
     5881 gaaagccgct ggttttaaag aagtgaaata atctgtcggc ggccatgttt cgcatggccg
     5941 ccatacccgt cttagctttt cttcacatgc tggcgcgctg ccagtccacg cagaaaataa
     6001 cgtaaaaatt gatcgccgca ttcgcggaag tttttatgat ccggtgcgcg catcatcgct
     6061 gtaatttccg gcatcgaaac gcggaactgc tgttcggtga ggatcgccag aatgtcatcg
     6121 gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt
     6181 tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat caggccattg
     6241 aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt
     6301 agccagacgg cgatctgttc cgcggtggct tcgacattac ccagcgccag aatacgcacc
     6361 aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc gttacttagc
     6421 atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt acaggccaat
     6481 cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc cattacgcaa
     6541 aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg cgaggttaac
     6601 cagatactga cgatggcagc gcagtagtgg tgtacgactt tccagggtac gtaatgtcaa
     6661 ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca tccgactgct
     6721 gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg tacaagggat
     6781 aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc tgcgctcctg
     6841 acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca gcagataatc
     6901 aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga gaaaaacaat
     6961 atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga tgcgcggcat
     7021 ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga tcccttccac
     7081 ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa atacacgcag
     7141 gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc ctcgtccctc
     7201 catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac gcttattcca
     7261 tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc cagcccactg
     7321 gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat caaatgttgc
     7381 ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga tgtcccatgt
     7441 ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg ctgggataat
     7501 tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc
     7561 agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga aggccgcttt
     7621 aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg
     7681 cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg attcacctgg
     7741 gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc atattgcccg
     7801 gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg gtttattgaa
     7861 ctgaataaac ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg ctgattttcg
     7921 ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa agagcaacga
     7981 taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc ttttaaggtg
     8041 taaggcgaag aaatcggttt gccaggtaaa tggtggtcgt caccaattcc ggtaaaggcc
     8101 agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg atacagcacc
     8161 tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc ttccgtcgag
     8221 gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt
     8281 ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac catcattggc
     8341 gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat cgccaggatg
     8401 atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat ggggttaaag
     8461 actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc gagcaatcct
     8521 tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga atatcgatgt
     8581 aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa gccgcccatt
     8641 accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg
     8701 cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg cagacgaacc
     8761 gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc aatgactaaa
     8821 aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat acccgcaaac
     8881 cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct ttgaactctt
     8941 gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa aataaaatca
     9001 ccctatagat gcacaaaaaa cgggcaaaac tacctggttc gcaaaactgc gtctaaagtt
     9061 aaaccgggac ctcgcgagca agggtgagac gatggcgctt tacacaattg gtgaagtggc
     9121 gttgctttgt gatattaacc ctgtcacgtt acgcgcgtga cgcaacattt ttaaaaatcg
     9181 acgaagatcg tccaggagcg ccgtttttca agggttggat agatgattcg tttaccatgt
     9241 cgacgaactt cgacgaattt cgccgataaa aagtgtccca tacatgcccc attgatgccc
     9301 cataaacaat atcaggaatt gacactggtt atccatacag ataaaaataa cacctgtaca
     9361 taaccacagt tacaggaggg tgttatgcgt gttgagatct gtatcgctaa agaaaaaatc
     9421 actaaaatgc caaacggtgc tgtggatgcg ttaaaggaag aattaacccg acgcatcagt
     9481 aaacgttatg acgatgtaga ggtgatcgta aaagccacca gcaacgatgg cctttctgtt
     9541 acacgcaccg cagataagga ttctgcaaaa acatttgttc aggagactct gaaagatacc
     9601 tgggagtctg c
//
LOCUS       DAZRUF010000158         9501 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZRUF010000158 DAZRUF010000000
VERSION     DAZRUF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.158"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHXOL_003026"
     CDS             <1..108
                     /locus_tag="ACHXOL_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR6809952.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHXOL_003027"
     CDS             complement(149..1231)
                     /locus_tag="ACHXOL_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR6809953.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHXOL_003028"
     CDS             complement(1228..3060)
                     /locus_tag="ACHXOL_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR6809954.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHXOL_003029"
     CDS             complement(3275..3631)
                     /locus_tag="ACHXOL_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809955.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="ACHXOL_003030"
     CDS             complement(3689..4111)
                     /locus_tag="ACHXOL_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR6809956.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHXOL_003031"
                     /pseudo
     CDS             complement(4127..4852)
                     /locus_tag="ACHXOL_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(4874..5620)
                     /locus_tag="ACHXOL_003032"
     CDS             complement(4874..5620)
                     /locus_tag="ACHXOL_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR6809957.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHXOL_003033"
     CDS             complement(5627..6691)
                     /locus_tag="ACHXOL_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR6809958.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHXOL_003034"
     CDS             complement(6763..7188)
                     /locus_tag="ACHXOL_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR6809959.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="ACHXOL_003035"
     CDS             complement(7185..7448)
                     /locus_tag="ACHXOL_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR6809960.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="ACHXOL_003036"
     CDS             7560..8060
                     /locus_tag="ACHXOL_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR6809961.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="ACHXOL_003037"
     CDS             8080..8274
                     /locus_tag="ACHXOL_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR6809962.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="ACHXOL_003038"
     CDS             8274..8564
                     /locus_tag="ACHXOL_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR6809963.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8844..8996
                     /locus_tag="ACHXOL_003039"
     CDS             8844..8996
                     /locus_tag="ACHXOL_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR6809964.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            9008..9373
                     /locus_tag="ACHXOL_003040"
     CDS             9008..9373
                     /locus_tag="ACHXOL_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6809965.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT         2443 a         2490 c         2017 g         2551 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatt tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ctggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc cactacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatgc tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaat aatgatgcta ttgtttcagg cgtcaaccca ccccgcccca cagaatgcag
     8701 ggcaatactt cgagttacca gacagtggtc aggggttaag tagccagccc gaggcgtaag
     8761 aacatgacgg cagggttcaa tctttaacta tgcagcaggt tttttgttcc gccaccccgg
     8821 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gaatctctgg
     8881 tgtgcggcgt gttccctaac caggatggaa cgtttaccgc catgacgtat accaaaagca
     8941 aaacgtttaa aaccgaatct ggcgcgcgtt gctggttagc cagaaacact gactgatgag
     9001 gctgacgatg gaatttaaag atttaccgtc tgatgtacag aaaacagcag ctcatacatt
     9061 gcattctgtg ctgcgagaaa tcgggaaaga tattgcaagc gagccagcaa aagatctggc
     9121 ccggaaaatc aagaccgctt tcgttgagct ttataatgtt ggcactgact ctgaaactgt
     9181 cgagaccaag accgtaagtt caccaatatt ctcacttgag ccaagcgtat tatcaggtga
     9241 aatatgcacc gagatttcca gcgaactgct tccggtaata cgagaagcaa ttcgccgtcg
     9301 cggtttggat ggaagttatg atcatgacgt tctgcaagtt ctcaggacaa tggtgacttc
     9361 actggggatt tgatccctgc gtcctctaag cttttgatat agcggtcctc caagaagcgg
     9421 taaattttgt cgaaatcatc ttttccctct ggtttattca gaggattaca ggacccgtaa
     9481 gtagcggcat acatttcaac c
//
LOCUS       DAZRUF010000159         9496 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZRUF010000159 DAZRUF010000000
VERSION     DAZRUF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.159"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            122..952
                     /locus_tag="ACHXOL_003041"
     CDS             122..952
                     /locus_tag="ACHXOL_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR6809966.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHXOL_003042"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHXOL_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR6809967.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="ACHXOL_003043"
     CDS             complement(2081..3541)
                     /locus_tag="ACHXOL_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR6809968.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHXOL_003044"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHXOL_003044"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR6809969.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..6489
                     /gene="gntP"
                     /locus_tag="ACHXOL_003045"
     CDS             5146..6489
                     /gene="gntP"
                     /locus_tag="ACHXOL_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR6809970.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDTATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGQLELVNSVVGLIIVLIISMVA"
     gene            complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHXOL_003046"
     CDS             complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHXOL_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR6809971.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHXOL_003047"
     CDS             complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHXOL_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFR6809972.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHXOL_003048"
     CDS             complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHXOL_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR6809973.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHXOL_003049"
     CDS             complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHXOL_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR6809974.1"
                     /translation="KSYCLNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLA
                     GVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQL
                     YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYAT
                     EYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPL
                     PFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQ
                     QLLTQLSAECR"
BASE COUNT         2260 a         2483 c         2376 g         2377 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaacca gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctgcaat ccttgataca gcaactggtc agctggttgg cgtgaatccg
     6301 gcgctgctgg tgctagcgac ggctgcgggt tccaacaccc tcacccacat taatgatgcc
     6361 tcattctggc tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg
     6421 ggtcagctgg agctggtcaa ctccgtggtt gggctgatta ttgtgttgat tatcagcatg
     6481 gtagcgtaaa agagcaccag agcctgccat tggcaggctc tggtcggatt gtcggcaaag
     6541 tgcgctttgt ttattcttat ctggcctaca aagggctaac gtgcgggttt gtagatagcg
     6601 tagcgcatca ggcaatattg cgtacctgca ttagcaatgt cctgtgattt ctttattgat
     6661 aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc cctccagtac
     6721 gtgcgtaatt tgccgttaat cccagactta ccgccgaagt ccctactgct cctaacgata
     6781 ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg acgccctgcg
     6841 ctggtgaaaa cgaggcggta ttggtgaaaa tcgaattgcc cgcatctgcg gttgtgccgg
     6901 agaggtaata tcccaggttt tggcttttcg cacaataaac ggtaagagga attggcactg
     6961 aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatcg cagccgccag
     7021 tgggcaccac cacatcatta ttggcgtaaa tattccacac aaactgaaaa tcatcgctgt
     7081 tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgaaccagct ttaatcgcca
     7141 ccccgccagc actgctcaca ggcgtcaaat aaagcgccac cggccacggc ttatccgttc
     7201 tcgaattata aacaacccgc ggcgtttcgc tggtggtagg aaatggatag ctactgccac
     7261 tatattttac ggtcccggaa aaattagata acacgccgcc ataagccgag cctcgttgca
     7321 gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt tgcgtcgaaa
     7381 gatccacgac caggttttgc cccacattca cggcaggcgc aaggtttaca taaacattag
     7441 cgctgccacc gccaataggg atagcggtgc cattggcggt tttacaggcg aatgaccagg
     7501 catttaccga ccagcccatc agcagtacag caaacagggt aataactcgt ttcattacaa
     7561 tcatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg aatggttccc
     7621 tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg cgctgattgt
     7681 gaggaatcat ccacctgaac tgttttggtt gcgccactat tcaatgtgtt gccactgtca
     7741 tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata tccggtactg
     7801 tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca attagtcaac
     7861 tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact gaaagaataa
     7921 agatcgccga gatctaccgt ggcattggtg gtggaaaccg tgcacggttt ggcgacgacc
     7981 ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc gagcgccaac
     8041 attgccgcca ataaataccc acgtttgcgc catttcatga gcatctccag ttactgatat
     8101 tcaagagtga aggtagccgt ggcattgata tgccccgcag tgacaggcac ctgtgtcgcc
     8161 attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt cgtccacgaa
     8221 agcgcggacg atggggcatt aaggggtatc tgattttgtt gctcattcag aagctgtatt
     8281 cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc attgtggcta
     8341 tctgcaacac cggtaaaccc aacttttacg gcagaaacgg cattaccaca gggtgacagc
     8401 aaaatacgaa atggaacgac aggagtcgtc gcgccaatgt tgttaaattg cttcgccgcg
     8461 ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca gccgttatct
     8521 ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aagcgcgact caccgccaac
     8581 cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc ttaacgacat
     8641 tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg ataattggcg
     8701 acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc taaaggcatt
     8761 ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct actctctgat
     8821 gtcaccatcg ccccaaacgg cagcggctta ttattgtggg ttagcgtcat gagcagtttt
     8881 atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg aacaacgtta
     8941 gcgaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag cgctactctg
     9001 ttttcccgat attcagtggc ataaggcagc acggcataac cgcgccagtc ggtacgcacc
     9061 cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag caccaccgta
     9121 tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccagtacccc accgctgact
     9181 ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatgttggc attgccgtaa
     9241 ccaccgcgat aattcagcgt ggcgtagcct gtacttccgc tattaccatc acctcccccg
     9301 gcgtaaccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt accgtataca
     9361 ccggccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtaact ggcactggca
     9421 tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat attgacgtta
     9481 aggcagtatg actttc
//
LOCUS       DAZRUF010000160         9402 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZRUF010000160 DAZRUF010000000
VERSION     DAZRUF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.160"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            206..1072
                     /gene="folD"
                     /locus_tag="ACHXOL_003050"
     CDS             206..1072
                     /gene="folD"
                     /locus_tag="ACHXOL_003050"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR6809975.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1074..1286
                     /gene="ybcJ"
                     /locus_tag="ACHXOL_003051"
     CDS             1074..1286
                     /gene="ybcJ"
                     /locus_tag="ACHXOL_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR6809976.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1394..1915
                     /locus_tag="ACHXOL_003052"
     CDS             1394..1915
                     /locus_tag="ACHXOL_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR6809977.1"
                     /translation="MPTVITHAAVPLCIGLGLSSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSCYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="ACHXOL_003053"
     CDS             complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="ACHXOL_003053"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR6809978.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3510..4004
                     /gene="ppiB"
                     /locus_tag="ACHXOL_003054"
     CDS             3510..4004
                     /gene="ppiB"
                     /locus_tag="ACHXOL_003054"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR6809979.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4007..4729
                     /gene="lpxH"
                     /locus_tag="ACHXOL_003055"
     CDS             4007..4729
                     /gene="lpxH"
                     /locus_tag="ACHXOL_003055"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR6809980.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4847..5356
                     /gene="purE"
                     /locus_tag="ACHXOL_003056"
     CDS             4847..5356
                     /gene="purE"
                     /locus_tag="ACHXOL_003056"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR6809981.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5353..6420
                     /gene="purK"
                     /locus_tag="ACHXOL_003057"
     CDS             5353..6420
                     /gene="purK"
                     /locus_tag="ACHXOL_003057"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR6809982.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6557..7450)
                     /locus_tag="ACHXOL_003058"
     CDS             complement(6557..7450)
                     /locus_tag="ACHXOL_003058"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR6809983.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7447..8100)
                     /locus_tag="ACHXOL_003059"
     CDS             complement(7447..8100)
                     /locus_tag="ACHXOL_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR6809984.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8111..9370)
                     /locus_tag="ACHXOL_003060"
     CDS             complement(8111..9370)
                     /locus_tag="ACHXOL_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR6809985.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2091 a         2667 c         2494 g         2150 t
ORIGIN      
        1 ttagacaagg aaataagtca gttccagatt tacaacgcca tcatggacga aaaatgaagc
       61 tttcaatctc agcgacggtg cgccccacct tcgcaagagg tcgcttcacg cgataaatct
      121 gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata ggcgcatccc cttcgaccta
      181 cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc
      241 gcagcaggtg cgctctgaag ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg
      301 ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc
      361 aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga
      421 aaccaccagc gaagcggagc tgttggagct tatcgatgcg ctgaatgccg acaacaccat
      481 cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga
      541 acgtattcac ccggacaaag acgtagacgg tttccatcct tataacgtcg gtcgcctgtg
      601 ccagcgcgcg ccgcgtctgc gcccctgcac ccctcgcggc atcgtcacgc tgcttgagcg
      661 ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg
      721 tcgtccaatg agcatggaac tgttgctcgc aggttgcacc actaccgtga ctcaccgctt
      781 cactaaaaat ctgcgtcacc acgtagaaaa tgccgatcta ctgatcgttg ccgtcggcaa
      841 gccgggcttt attcctggtg actggatcaa agaaggcgca attgtgattg atgtcggcat
      901 caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg
      961 cgcctcatac attacgcctg ttccgggcgg cgttggcccg atgacggttg ccacgctgat
     1021 tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga
     1081 cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct
     1141 ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg aaagccgacg
     1201 gcgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgcag
     1261 gtcacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct
     1321 cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc
     1381 cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttcccctat gcattggttt
     1441 agggttgagt tcgaaagtca tccctccgcg tctgttattt gccggaatca tcctggcgat
     1501 gctgcccgac gccgacgtat tgtcgtttaa atttggcgtt gcttacggca atgtttttgg
     1561 tcatcgcggg tttacccatt cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgct
     1621 gattggacgc cgatggttca gggctgggct gattcgctgc tggctgtttt taaccgtctc
     1681 gttgctatcg cacagcatgc tggattcggt aaccactggc ggtaagggtg ttggctggct
     1741 gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag tcgcgccgtt
     1801 tgcattgtct tgttacacca cgccgtacgg gcatcaggtg attatttctg aattgatgtg
     1861 ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac gataaccgga
     1921 tgcgaaaact cgcatccggc aatagcgcaa ttacttacga cgccaggtgg tcccttgcgg
     1981 gccatcttcc agaacgatcc ccatctcgtt aagacggtca cgcgccgcat ctgccgccgc
     2041 ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa tctcagccac
     2101 ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt gctccagcag
     2161 gcctaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg ctgccatatc
     2221 ttctgctttc agacggttta cttcacgcgc catatcaaac agtacggaat aggcttccgg
     2281 ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc
     2341 aggagcaacg gttttttctg tgccgcgcag ggcagtgtag agacgctcca gcgccgcacg
     2401 cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc ccgacatcag
     2461 gaagtaacgc acggtttcag catcgtagta tttcagcaca tcgcgcacgg taaagaagtt
     2521 acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt gcatccagta
     2581 gttaacatac tgaccatcat gggcacaggt ggactgcgcg atttcattct cgtggtgcgg
     2641 gaacatcagg tcagaaccgc cgccgtggat atcaaagtgg ttacccagct gcttgcagtt
     2701 cattgccgaa cattcaatgt gccagcctgg acggcccgcg ccccacggag acggccagct
     2761 cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc gtttgtcgtc
     2821 gaccacgtca acgcgcgcgc ctgcctgcag ctggtcgaga tcctgacgcg acagcacacc
     2881 ataagtcgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgccg cataagcgtg
     2941 acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt gcgtcgcgcg
     3001 cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat
     3061 catgcgatcc accagcgcca caaagctttc gccattttca ttggcacgtt tgatgatttt
     3121 gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga aacgcagata
     3181 gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccgatatgac agagatcgta
     3241 aacggtgatt ccacacacat acatgccgac ttccccggcg tgaataggct taaattcctc
     3301 tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt agacatgtgt
     3361 gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata cattgcgtga
     3421 tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa ctgggttgaa
     3481 gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat cacggcgata
     3541 ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc ctggactact
     3601 gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc tttatgattc
     3661 agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg
     3721 aagccaacaa cggtctgaaa aatacccgtg gtacgctggc aatggcccgt actcaggctc
     3781 cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc ctgaacttct
     3841 ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt gaaggcatgg
     3901 acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc
     3961 caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt
     4021 tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt ttctgcgttt
     4081 tttagcgggg gaagctcgca aggccgacgc gctgtatatt cttggcgatc tgtttgaagc
     4141 gtggattggc gacgacgagc ccaatccact ccatcgccag atggcggcag cgatcaaagc
     4201 ggtgtccgat tctggcgttc cctgttattt cattcatggc aaccgtgatt ttctgctcgg
     4261 caaacgcttt gcccgtgaaa gtggcatgac gttattgccg gaagaaaagg tgctcgaact
     4321 ttatggccgc cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta
     4381 tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc tcgccctgcc
     4441 gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgagcg aacagtaaag aagccaacag
     4501 cagtaaatcg ctggcgatca tggacgttaa ccaaaacgcg gtagtcagtg cgatggaaaa
     4561 acatcaggtg caatggctga tccacggaca tacccatcgc ccggcggtac atgaacttat
     4621 cgccaaccag caacctgctt ttcgcgtggt actcggtgcc tggcatacgg aaggttcgat
     4681 ggtgaaagtc acggcggatg acgttgagct gattcatttt ccgttttaaa aaaaccgcaa
     4741 ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg ctattctctg
     4801 tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt cttcccgcaa
     4861 taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt
     4921 cgccgccgaa atcttcgaaa tcctgaatgt cccgcaccac gttgaagtgg tttctgctca
     4981 ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt
     5041 gattattgcg ggcgcaggtg gcgcagcgca cctgccaggc atgattgccg ccaaaacgct
     5101 ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg atagcctcta
     5161 ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg
     5221 cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata aagagctaca
     5281 ccagcgtctg aatgactggc gcaaagcaca aactgatgaa gtgctggaaa acccggaccc
     5341 gcgaggtacg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg
     5401 cgtcaggcag gcgaaccgtt aggcattgct gtctggccag tcgggctgga cgctgaaccg
     5461 gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg gccggaaacc
     5521 gcattaaccc gcgagctggc gcgccatccg gcctttgtga accgcgatgt gttcccgatt
     5581 attgctgacc gtctgactca gaagcagctt ttcgataagc tccacctgcc gaccgcgccg
     5641 tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg
     5701 gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggcg tttacgcgcc
     5761 aatgaaaccg aacagttacc ggcagagtgt tacggcgaat gtattgtcga gcagggcatt
     5821 aacttctctg gtgaagtgtc gctggttggc gcgcgcggct ttgatggcag caccgtgttt
     5881 tatacgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt cgcttttccg
     5941 caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag
     6001 ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg tctgctgatc
     6061 aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaatgg tgccagcatc
     6121 agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca accagtagtg
     6181 aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga ctggctgaaa
     6241 ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg taaagtgggg
     6301 catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga agccttaatc
     6361 ccgctgctgc cgccagagta tgccagcggc gtgatttggg cgcagagtaa gttcagttaa
     6421 gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt accgctccgg
     6481 tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt gcaccgattg
     6541 ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt
     6601 caattcgcga taacgccccg atccacgcgg gtttaccacg gcttctgaca taaccactca
     6661 ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct aactcatccg
     6721 gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat accgcatcag
     6781 catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac gcagcggcga
     6841 gatctttatc aatcactgct tcactccctg ccccatcttc cgtcacaggc acacctccgc
     6901 cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga
     6961 gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca tcacgtttca
     7021 tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa accggaccaa
     7081 taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg cgcgtcagca
     7141 ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc atatagccaa
     7201 tcatcccctg gctttccgcc accagcacat ccagcggata cggttctacc tctttccacg
     7261 ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca atcgccagcc
     7321 gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg cgatattgat
     7381 tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca
     7441 gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca gcagcgtatc
     7501 tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg
     7561 gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt gcgcggcata
     7621 acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg aataggcaaa
     7681 aaactggcgt aactcagcag gcagcggatc gcctgccgcc aacgccagtg gcccgaaaag
     7741 cccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgcaggcgg
     7801 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
     7861 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
     7921 ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt
     7981 taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc ccgctaaccg
     8041 ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga tgatcgtcat
     8101 tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaaac
     8161 acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc gatccccgcc tctttatggg
     8221 cgataccggt attgatgagt ggcgtaatgt cggtttctac cacgcgacga atgtccagtc
     8281 ccaggcacgc gccctgaaaa tcccagcctg ggatctgcaa ctgcatattg cgctcaaggt
     8341 aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc gcaccgacaa
     8401 agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa
     8461 tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac
     8521 cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc
     8581 cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc
     8641 cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca
     8701 cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg cgagcaattt
     8761 gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga tggaactcat
     8821 cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc tccatgcgcc
     8881 ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga
     8941 catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt
     9001 acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa
     9061 taccgcccat tggaccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct
     9121 gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac agacatgcgc
     9181 ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt
     9241 gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca
     9301 gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg
     9361 atgtaaacat gggaacccct tattgcaaac gttctaataa ac
//
LOCUS       DAZRUF010000161         9226 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZRUF010000161 DAZRUF010000000
VERSION     DAZRUF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9226
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.161"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            173..454
                     /gene="drpB"
                     /locus_tag="ACHXOL_003061"
     CDS             173..454
                     /gene="drpB"
                     /locus_tag="ACHXOL_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR6809986.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            494..1150
                     /locus_tag="ACHXOL_003062"
     CDS             494..1150
                     /locus_tag="ACHXOL_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR6809987.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            1217..2635
                     /gene="dcm"
                     /locus_tag="ACHXOL_003063"
     CDS             1217..2635
                     /gene="dcm"
                     /locus_tag="ACHXOL_003063"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR6809988.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2616..3086
                     /gene="vsr"
                     /locus_tag="ACHXOL_003064"
     CDS             2616..3086
                     /gene="vsr"
                     /locus_tag="ACHXOL_003064"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR6809989.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3075..3996)
                     /gene="yedA"
                     /locus_tag="ACHXOL_003065"
                     /pseudo
     CDS             complement(3075..3996)
                     /gene="yedA"
                     /locus_tag="ACHXOL_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            4169..5086
                     /locus_tag="ACHXOL_003066"
     CDS             4169..5086
                     /locus_tag="ACHXOL_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR6809990.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5165..5347
                     /locus_tag="ACHXOL_003067"
     CDS             5165..5347
                     /locus_tag="ACHXOL_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR6809991.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            5518..7212
                     /gene="dgcQ"
                     /locus_tag="ACHXOL_003068"
     CDS             5518..7212
                     /gene="dgcQ"
                     /locus_tag="ACHXOL_003068"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR6809992.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(7209..8024)
                     /locus_tag="ACHXOL_003069"
     CDS             complement(7209..8024)
                     /locus_tag="ACHXOL_003069"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR6809993.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8322..8549)
                     /gene="yodD"
                     /locus_tag="ACHXOL_003070"
     CDS             complement(8322..8549)
                     /gene="yodD"
                     /locus_tag="ACHXOL_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR6809994.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            8712..8900
                     /gene="dsrB"
                     /locus_tag="ACHXOL_003071"
     CDS             8712..8900
                     /gene="dsrB"
                     /locus_tag="ACHXOL_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR6809995.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(8944..>9226)
                     /gene="rcsA"
                     /locus_tag="ACHXOL_003072"
     CDS             complement(8944..>9226)
                     /gene="rcsA"
                     /locus_tag="ACHXOL_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR6809996.1"
                     /translation="DILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQI
                     SDQMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
BASE COUNT         2252 a         2224 c         2494 g         2256 t
ORIGIN      
        1 tattgctgtt tttccagtaa ttattgccgt tgcgcaaaat ttatctattt gttcaaaaaa
       61 tgattgagtc ttgactggct catccaatgt ggaaaaatgt gacttttatc acataatagt
      121 actaagtctg aattttccgg gttatctcaa aatggaatac ggttcgacaa agatggaaga
      181 gagactctct cgcagccctg gcggcaaatt ggctttgtgg gctttctata catggtgtgg
      241 ctatttcgtc tgggcaatgg cgcgttacat ctgggtgatg agccggatcc ctgatgcccc
      301 ggtgtcggga tttgaaagcg atttaggctc taccgcggga aagtggttag gggcactggt
      361 cggattttta tttatggctc tggtcggggc attgttaggc agcattgcct ggtacacccg
      421 acctcgtccc gcacgtagca gacgctatga ataaaaactg ctaaggtggc gactctttgg
      481 caacggagcg cgtatggact tacaacactg gcaggcacag ttcgaaaact ggttgaaaaa
      541 ccatcatcag caccaggacg cggcacagaa gctggcggca gatgacgatg tcgatatgct
      601 ggtgatttta accgcctgtt attttcacga tattgtgagc ctggcgaaaa atcatccgca
      661 aaggcagcgt tcttcaatcc tggcggcaga agagacgcgt cgcctgctcc gtgaagagtt
      721 cgagcaattt ccggcggaga aaatcgaggc cgtttgtcat gccattgccg ctcacagttt
      781 cagcgcgcaa atcgccccct taacaacgga agctaaaatc gtccaggatg cggatcggct
      841 ggaagccctg ggggcgattg gcctggcgcg cgtgtttgcc gtttcagggg cattgggcgt
      901 ggcgctgttt gatggtgaag atccgttcgc acagcatcgc ccgcttgatg ataaacgcta
      961 cgcgctggat catttccaga ctaagttgct gaagctaccg caaaccatgc aaaccgcaag
     1021 aggcaagcag ctggcgcagc acaacgcgca ttttttagtc gagtttatgg cgaagctcag
     1081 tgccgaactg gcgggggaga atgaaggtgt cgatcacaag gtgatagatg cgttttcacc
     1141 cgcgggataa gcgcgtggcc ctaaatggct gtaattatgt taacctgtcg gccatctcag
     1201 atggccggtt aaatctatgc aggaaaatat atcagtaacc gattcataca gcaccgggaa
     1261 tgccgcacag gcaatgctgg agaaactgct gcaaatttat gatgttaaaa cgctggtggc
     1321 gcagcttaat ggtgtgggtg agaatcactg gagcgcggca attttaaaac gtgcgctggc
     1381 gaatgactcg gcatggcacc gtttaagtga gaaagagttc gcccatctgc aaacgttgtt
     1441 acccaaacca tcggcacatc atccgcatta tgcgtttcgc tttatcgatc tatttgccgg
     1501 aattggcggc atccgtcgcg gttttgaatc gattggcgga caatgcgtgt ttaccagcga
     1561 atggaacaaa catgcggtac gcacttataa agccaaccat tattgcgatc cggcgacgca
     1621 tcattttaat gaagatatcc gcgacatcac cctcagccat aaagaaggcg tgagtgatga
     1681 ggcggcggcg gaacatattc gtcaacacat tcctgaacac gatgttttac tggccggttt
     1741 cccttgtcag ccattttcgc tggctggcgt atcgaaaaag aactcgctcg ggcgggcgca
     1801 cggttttgcc tgcgataccc agggcacgct gttttttgat gtggtacgca ttatcgacgc
     1861 gcgtcgtccg gcgatgtttg tgctcgaaaa cgtcaaaaac ctgaaaagtc acgaccaggg
     1921 taaaacgttc cgcatcatca tgcagacgct ggacgaactg ggctatgacg tggctgatgc
     1981 agaagataac gggccggacg atccgaaaat catcgatggt aaacattttc tgccgcagca
     2041 ccgtgaacgc atcgtgctgg tgggttttcg tcgcgatctt aatctgaaag ccgattttac
     2101 tctgcgtgat atcagcgaat gtttccctgc acagcgagtg acgctggcgc agctgttgga
     2161 cccgatggtc gaggcgaaat atatcctgac gccggtgctg tggaagtacc tctatcgata
     2221 tgcgaaaaaa catcaggcgc gcggtaacgg cttcggttat ggaatggttt atccgaacaa
     2281 tccgcaaagc gtcacgcgta cgctgtctgc gcgttattac aaagatggcg cggaaatttt
     2341 aatcgatcgc ggctgggata tggccacggg tgagaaagat tttgacgatc cgctgaatca
     2401 gcaacatcgt ccacgtcggt taacgcctcg ggaatgcgcg cgcttaatgg gttttgaagc
     2461 gccgggagaa gcgaaattcc gcattccggt ttcggacact caggcctatc gccagttcgg
     2521 taactcggtg gtcgtgccgg tctttgccgc ggtggcaaaa ctgcttgagc caaaaatcaa
     2581 acaggcggtg gcgttgcgtc agcaagaggc acaacatggc cgacgttcac gataaggcca
     2641 ctcgcagcaa aaatatgcgc gcgattgcca cgcgtgatac ggcgattgag aagcgcctcg
     2701 ccagtctgtt aaccgggcag ggcctggcgt ttcgcgttca ggacgccagt ctgcccggaa
     2761 gcccggattt tgtcgttgat gaatatcgct gcgtgatatt tacccatggc tgcttctggc
     2821 atcatcatca ctgctatctg tttaaagtgc ctgcgactcg aaccgagttc tggctggaga
     2881 agataggtaa aaatgttgaa cgcgatcgcc gcgatatcag tcgtttgcag gaactcggct
     2941 ggcgcgtact gattgtctgg gagtgtgcat tacgtgggcg cgagaagctg acggatgaag
     3001 cgcttaccga gcgtctggaa gagtggatct gcggcgaagg tgccagcgcg cagatcgaca
     3061 cgcaggggat tcatttactc gcttgatgcg tcctgaataa ctggcgcaac tacgggtttt
     3121 gccgggaaga gatattttcc caacgtgacc agtaccaccg cgaagacaat tacgccgagc
     3181 gccagccatt caatcttcga cagcgtctct ccgcccagtc ccgtacccag caagaccgcg
     3241 accaccgggt taacgtaggc gtagctggtg gcgagagccg gactgacatt acggattaaa
     3301 tacatataag cgttgatggc gataatcgaa ccaaacagcg ccagatagcc gaccgcaagg
     3361 aagcctgaaa gggaagggag cgccgtcagt ttttcacctg caatcatcga cgcgatcatt
     3421 aacaccacgc ctgccgccag catctcaatc gcacccgcca tcatccctac aggtaaggta
     3481 atgcgcgagc cataaaccga gccaaacgcc cagctaatcg agccgattaa aatcagaatc
     3541 gcgccccacg gattgccgct taaatttcca ccgctattga gcatgatgat tccggcaagc
     3601 ccaatggcga tacccaccca ttccagtttg cgcgttttaa tgccaaacag gcggctgaag
     3661 caccagggta aagaggggca cggttgcaac cacaacggcg gcgatgccgg aaggaacatt
     3721 ttgatgctcg gcaaccgtca ccatgccatt accgacagcc agcaataaca ggccaatcag
     3781 cgcggcattg agcagcggac gtagcggggg gagcttgtgt ccgcgcagta gcaaaaatgc
     3841 catcaataaa ataccggctg ccaggaatcg aacgcctgcc atcattaacg gaggccagct
     3901 ttccacgcca atccgaatga caaaataggt tgagccccaa atgatataca acgcaaacag
     3961 cgcgccaaaa agcggtaaca actggcggaa acgcataatc cctcacggtg gaaataaaaa
     4021 ggtggttcat agtaaacgtg aaaatcattc tgctggcgag agatataatt gcacttgatt
     4081 gtaaaaaaaa tgttgacctg tgaagcaagt atcagagctg ccgtttttgc ttcatactta
     4141 cacccttcaa caataaaaat gagagggaat gcttttggcc gggagtagtt tactgacgtt
     4201 gctcgatgat atcgccacac tactggacga tatctccgtg atgggcaaac tggcggcgaa
     4261 gaaaaccgcc ggtgtattag gggatgactt atcgctcaat gcgcaacaag tttcaggcgt
     4321 gcgggccaac cgggaacttc ccgtggtctg gggcgtggcg aaaggatcgc tgattaataa
     4381 agtgattctg gtgccgctgg cgctgatcat cagtgcgttt atcccgtggg cgattacgcc
     4441 tctgttgatg attggtggcg cgtttctctg ctttgaagga gtagagaaag tgctgcatat
     4501 gctggaggcg cgtaaacata aagaagatcc ggcgcagagc cagcagcgtc tggagaagct
     4561 ggcggcgcag gatccgctga agtttgaaaa ggacaaaata aaaggggcga ttcgtaccga
     4621 ttttatattg tctgccgaaa tcgtcgccat cacgctgggg attgtggccg aagcgccgtt
     4681 gcttaatcag gtgctggtgc tttcaggcat cgcgctggta gtgaccgtgg gcgtctacgg
     4741 tctggtaggg gttatcgtta agattgatga cctggggtat tggctggcgg aaaaatccag
     4801 cgcgctgatg caggcattag gtaaagggtt attgatcatt gcgccctggt tgatgaaggc
     4861 gttatcgatt gtcggcacgc tggcgatgtt cctcgtcggc ggcggtattg tggtacatgg
     4921 tattgcgccg ctgcatcacg ccattgaaca tttcgccggg cagcaaagtg cagtggtggc
     4981 gatgatatta ccgactgttt taaatctgat tcttggattt atcatcggcg gcatcgtggt
     5041 gctgggagtg aaagccgtag cgaaaatgcg cggtcaggca cattaatact cgtacaggct
     5101 attatgcaaa attcgccatc ttcaactaag gtgaacaagt ttcactcgaa aaaaggaggc
     5161 gagtatgagc tttatggtta gtgaggaagt tacggttaaa gagggcggcc cgcggatgat
     5221 tgtcactgga tactccagcg gtatggttga gtgtcgttgg tatgacggtt acggggtcaa
     5281 gcgggaagct tttcatgaaa ccgagcttgt tccgggggag gggagtcgtt ctgcggaaga
     5341 agtttgaaag ggtatcatta acaccaggcc ggatcacttc cggcctggtg tgccaatgcc
     5401 tgcaatgaat gatagtcgcg tcttatcatg ccaccattat aaaattatcg ctgttttgcg
     5461 gtacgctatc gccaggttat gccagaatca taaaaaagca ggttgggagt cgtcagggtg
     5521 cagcacgaga caaaaatgga aaaccagagc tggttgaaaa aactcgcacg ccgcctgggg
     5581 cctggtcatg tcgttaatct ctgctttatc gtggtattgc ttttttccac cttgctgacc
     5641 tggcgtgaag tggtagtgct ggaagatgcc tatatctcca gccagcgtaa tcatctggaa
     5701 aacgtagcca acgcgctcga taagcatttg cagtataacg tcgacaaact gatctttttg
     5761 cgtaatggca tgcgcgaagc tctcgtagcg ccactggatt tcacttcact gcgtaatgct
     5821 gtaaccgagt tcgaacagca tcgcgacgag cacgcctggc aaattgaact caaccgacga
     5881 cgcaccctgt cagtcaatgg cgtatcggat gcattagtca gcgaggggaa tctcctgtct
     5941 cgcgaaaatg aaagcctcga caatgaaatt accgctgcac tggaagttgg ttacttgctg
     6001 cgactggcgc acaacacctc gtcgatggtt gaacaggcga tgtatgtctc gcgtgccgga
     6061 ttttacgttt cgacgcagcc gaccttgttt acgcgcaatg taccaacgcg ttattacggc
     6121 tatgtcaccc aaccctggtt tatcggccat tcgcaacgag aaaatcgtca ccgcgcggta
     6181 cgctggttta cttcgcaacc ggaacacgcc agcaatactg aaccgcaggt taccgtcagt
     6241 gttccggtag acagtaataa ctactggtat ggcgtgctgg ggatgagtat tcccgtgcgt
     6301 accatgcagc aatttttaag aaacgccatc gataaaaacc tcgatggtga gtatcagctc
     6361 tatgacagta agctgagatt tttgacctct tccaatcctg accatccaac agggaatatt
     6421 tttgatcctc gtgaactggc cttgctggcg caggcgatgg aacatgacac gcggggcggc
     6481 attcgtatgg acagtcgcta tgttagctgg gaacgtctgg accatttcga cggtgtgctg
     6541 gtgcgcgtcc atacgctaag cgaaggcgtg cgcggcgatt tcggcagtat cagcattgca
     6601 ttaaccctgc tgtgggcgct ctttaccacc atgttactca tctcctggta tgtgattcgc
     6661 cggatggtta gcaacatgta tgttctgcaa agctcgttgc agtggcaggc gtggcacgac
     6721 accttaaccc gtttatataa tcgtggcgca ctgtttgaaa aagcccgtcc gctcgcgaaa
     6781 ttgtgtcaga cgcaccaaca tcctttttct gtcattcagg tcgaccttga ccattttaaa
     6841 gcgattaatg accgctttgg tcatcaggcg ggcgaccgtg ttctttctca tgctgccgga
     6901 ttaattagca gttccttgcg tgcgcaggac gttgccgggc gggtcggtgg tgaggagttt
     6961 tgtgtgattc tgccaggcgc gagtctgacg gaggctgcgg aagtcgcaga acgtattcgc
     7021 ctgaagttaa atgaaaaaga gatgttgatt gctaagagta cgacgatacg catcagtgcc
     7081 tcgttggggg taagtagcag cgaggaaacc ggtgattatg attttgaaca actccagtca
     7141 ctggctgacc gtcggcttta tctcgctaaa caggccgggc gtaatcgggt atgcgcgagc
     7201 gataacgctt aacgagcgga gaaaaaatgg tccagccctt cacgccagcc ttccggtcct
     7261 tcacgctgcg ttcgccagac gcgggtcgga tcctcatcat gcagatgcac cccttcacgg
     7321 tttagccctt tcacaatcac cgcgtaatcc attacctcca gtaagggcgc atcgtttggc
     7381 ccatcgccca ggccaagtgt ggttgggcgt ttgcctgata attgttgata ggtcgcgata
     7441 atccagttgg cggcctgatc ttttccggca gaggcatcca ggacgtgcca gaagcgcgca
     7501 ccctgcataa actgtaagcc cagttcgttc agacgagcgg taaattgtgc catacgctcg
     7561 tcactgtcgc gccagattag cgttaccgac gcctcatgca gctgcgtcag cgccgcctgg
     7621 ctacggctta atcccgtcca ttcggcgatg gttgcatcgt cgacatcatc gaaagtcgtg
     7681 aatttaaaat gttctttctc gcgtagcgta tttaaaacct ggctgatttc gccatggcta
     7741 atacctgaga tgatgcgtgg aaaaccgtct atatcctgcc attgttcagc aagctggatc
     7801 actgcgccat tttcggcaat cagtggtaac ccttgtagcc ccaacgtttt ttgcaagtac
     7861 agcatttccg ctgaagtttt actgctacag agaatgacgg gaacattcgc ttcgcgtaaa
     7921 cggctgagcc agagggcagt cggttgccag tcataactat gactgtccag cagggtgcca
     7981 tcaagatcgc taaaaaccag tagtggttgt tgaattgaaa acatggtcgt gcagttctcc
     8041 ccttaccagg atgattctca aaacagcaca aataatcaaa gatgattttt tcgggagcga
     8101 tcccgttgac gccagagcca ccaaggggtg acgcacgtca catttctatt cataagtagc
     8161 gttaattatt catatcgcga atattttctt gtcagcgaaa aaaattgcgg ataaggtgat
     8221 gaacacatca gatttcctgg tgtaacgaat tttttaagtg cttcttgctt aagcaagttt
     8281 catcccgacc ccctcagggt cgggattttt ttattgtgca ttcaacgatt cacttcagag
     8341 acgctgaagt catgaatatc cagttcgaag gcatccgcca attcacgcca tgtatgatat
     8401 tcacgtccat cgacgctaac gtgttcagaa tcgttctggc tgcgatgaac ttcgctttcg
     8461 ctaatttcat tgatcgccgc cagcagggca tcgacatcga cgctgacctc acgttttgcg
     8521 gtatcgctgt actcttttgc ggttttcatc atctttcctc gcaaccgtta ctctgttgtt
     8581 gccgccagtc gttaagtccg cctggcatct cttaagtata gaccgtcgag aaaatcagca
     8641 ctcgcgcgcg cctggagctg ccagaaaaac gaaattgttc tacactggca caaagccaca
     8701 ggaggaaaac gatgaaggtg aatgatcggg taacagtcaa aacggatggc ggtccgcgtc
     8761 gtcctggcgt ggtactggca gttgaggagt ttagtgaagg cacaatgtac ctggtttcgc
     8821 tggaagacta cccgctcggc atctggttct ttaatgaagc agggcatcag gacggtatct
     8881 ttgtggagaa agcagagtaa tcttattcag cctgactggt gggaaaccac cagtcagaat
     8941 gtgttagcgc atgttgacaa aaataccatt agtcacatta tccgtcagtc ggacgacatg
     9001 gtagataacc tgtttattat gtgttttgat cttacgttta atattacctt tatgcgatga
     9061 aacggtcttg gctttgatat tcatttggtc agagatttga atggttccct gacctgccat
     9121 ccacattcgc aacatactcg attcggttcg gctcaatgat aacgtcggca tatttaaaaa
     9181 agaggttatc gttgtctctt ttttcagaat atcgccaagg atatcg
//
LOCUS       DAZRUF010000162         9128 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZRUF010000162 DAZRUF010000000
VERSION     DAZRUF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9128
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.162"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..279
                     /locus_tag="ACHXOL_003073"
     CDS             <1..279
                     /locus_tag="ACHXOL_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR6809997.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTNMTWSDADNQQVQLTT
                     QELEELATAMIQAIVERNDEIYRCQREMKEQLSLLPTLDEVRAFRPGD"
     gene            404..589
                     /locus_tag="ACHXOL_003074"
     CDS             404..589
                     /locus_tag="ACHXOL_003074"
                     /inference="COORDINATES: protein motif:HMM:NF021626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR6809998.1"
                     /translation="MDSVRCKNCNKLLFKGGFKHIEIKCPRCKRYIVISSAREHPTEL
                     YCGKREEITHSDKTLRY"
     gene            540..1235
                     /locus_tag="ACHXOL_003075"
     CDS             540..1235
                     /locus_tag="ACHXOL_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFR6809999.1"
                     /translation="MGKEKKSRILTKPCVIEYEGRIVGYGSRELRVETISCWLARTII
                     QTKHYSRRFVNNSWLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRM
                     WLHDDMPRNSESRAISYALKTIRLLYPSVEWVQSFADERCGRAGVVYQASNFDFIGSH
                     ESTFYELDGEWYHEITMNAINRGGKRGEYLRANKERAVVHKFNQYRYIRFLNKRARKR
                     LNTKLFRIQPYPK"
     gene            1467..2879
                     /gene="cycA"
                     /locus_tag="ACHXOL_003076"
                     /gene_synonym="dagA"
     CDS             1467..2879
                     /gene="cycA"
                     /locus_tag="ACHXOL_003076"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR6810000.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(2924..3586)
                     /gene="ytfE"
                     /locus_tag="ACHXOL_003077"
     CDS             complement(2924..3586)
                     /gene="ytfE"
                     /locus_tag="ACHXOL_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR6810001.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(3694..4659)
                     /locus_tag="ACHXOL_003078"
     CDS             complement(3694..4659)
                     /locus_tag="ACHXOL_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR6810002.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(4767..5627)
                     /gene="qorB"
                     /locus_tag="ACHXOL_003079"
     CDS             complement(4767..5627)
                     /gene="qorB"
                     /locus_tag="ACHXOL_003079"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR6810003.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            5716..6096
                     /locus_tag="ACHXOL_003080"
     CDS             5716..6096
                     /locus_tag="ACHXOL_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR6810004.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6214..8157)
                     /gene="cpdB"
                     /locus_tag="ACHXOL_003081"
     CDS             complement(6214..8157)
                     /gene="cpdB"
                     /locus_tag="ACHXOL_003081"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR6810005.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSNKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8347..9087
                     /gene="cysQ"
                     /locus_tag="ACHXOL_003082"
     CDS             8347..9087
                     /gene="cysQ"
                     /locus_tag="ACHXOL_003082"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR6810006.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2146 a         2267 c         2389 g         2326 t
ORIGIN      
        1 ggtcgtatct ggaatgctgg ccccgactca ctgggccgcc tgtccccggt agtcatgctg
       61 gcaaaatctg tcacagcaca aacaaatatg acgtggagtg atgcggataa tcagcaggtg
      121 cagctgacga cgcaggaact tgaggaactg gccacagcga tgatacaggc cattgttgag
      181 cgcaacgatg agatttatcg ctgtcagcgg gagatgaaag aacaactttc tctgttgccc
      241 acactggacg aggtgagagc gttcagacca ggcgattaaa taatcagttt gtatagttta
      301 tgacgatcga tagcatcata tcgatcgtcg gtttgcatca agtgtgatta aataccccca
      361 gccctccggg cagctatatc ctgttattac actgggggaa attatggact ctgttcgttg
      421 taaaaattgc aataaattgc tgtttaaagg aggttttaag catatagaaa tcaaatgtcc
      481 tcgctgtaaa cgttacattg tcatatcgag tgcccgggag catcccacgg agctgtattg
      541 tgggaaaaga gaagaaatca cgcattctga caaaaccctg cgttattgag tatgaaggcc
      601 ggattgtcgg ctatggttca agggagctgc gcgttgaaac catatcctgc tggctggccc
      661 gcacaatcat tcagacaaag cattattccc gccgttttgt gaataactcc tggttgcatc
      721 tgggcgtatt cagtggacgt gatctggttg gtgttcttca gtggggatat gcccttaacc
      781 ccaactcagg gcgtcgcgtt gtgcttgaaa cggataaccg gggctatatg gaactgaacc
      841 gcatgtggct acacgacgac atgccccgca actctgaatc acgggccatc agctacgcgc
      901 tgaaaaccat caggttactg tatccgtcag tggagtgggt tcagtccttt gcagacgaac
      961 gttgcgggcg tgcaggtgtt gtgtatcagg cgtcaaattt tgattttatt ggcagccatg
     1021 aaagcacatt ctacgaactg gacggtgagt ggtatcacga gataacgatg aatgcgatta
     1081 atcgtggagg gaagcgcggc gaatatttac gagcgaataa ggagcgggcc gtggtacaca
     1141 aatttaatca atatcgttac atcaggttcc tgaacaagcg cgcaagaaag cgactaaata
     1201 ctaagctatt caggattcag ccttacccga agtgatccca cttaatgaat aaaaaagcag
     1261 cggttaatgc cgctgtttta tcttgaataa cttccatatg cgcgcttctg gcttatcttg
     1321 aaagaattcc atatctatct tgaataattt tgcgcgcttt aattgttacc taatatcgat
     1381 gagtcccgat acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac
     1441 acagacaggt acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc
     1501 aggctccggc tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg
     1561 ccattggcgg tgccattggt acggggttgt ttatggggtc cggcaaaacg attagccttg
     1621 ccgggccgtc gatcattttc gtttatatga tcatcggttt tatgctcttt ttcgtgatgc
     1681 gggcaatggg ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt
     1741 ccgatttact cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg
     1801 ttgtaaccgg tatggcagac gtggtggcga tcactgctta tgctcagttc tggttccccg
     1861 atctctccga ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg ctcaatctcg
     1921 ccaccgtgaa aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca
     1981 ttgtgtcgct gattgtcgtc ggcctggtta tggtggcgat gcactttcag tcaccgactg
     2041 gtgtggaagc gtcattcgcg catttgtgga atgatggcgg ctggttcccg aaaggtttaa
     2101 gtggcttctt tgccggattc cagatagcgg ttttcgcttt cgtagggatt gagctggtag
     2161 gtacaaccgc tgcggaaacc aaagatccgg agaaatcact gccacgcgcg attaactcca
     2221 ttccgatccg tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc
     2281 cgtggagttc ggtagtcccg gagaagagcc cgtttgttga actgtttgtg ttggtaggtt
     2341 tgcctgcggc tgccagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta
     2401 acagcggcgt cttctctacc agccgtatgc tgtttggtct ggcgcaggaa ggtgtggcac
     2461 cgaaagcgtt cgctaaactt tctaagcgcg cagtacccgc gaaagggttg accttctcgt
     2521 gtatctgtct gctcggcggc gtggtgatgt tgtatgtgaa tcctagcgtg attggcgcgt
     2581 tcacgatgat tacaaccgtt tccgcgattc tgtttatgtt cgtctggaca attatccttt
     2641 gctcgtacct ggtttaccgc aaacagcgtc ctcatctgca tgagaagtcg atctacaaga
     2701 tgccgctcgg caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtggtcgtgt
     2761 tgctgacact ggaagatgac actcgccagg cgctgttggt caccccgctg tggtttatcg
     2821 cactggggct gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac
     2881 cgcattatca tgctggatgg cgcaatgcca tccagctttt agattactca cccgccagcg
     2941 cgcgtgggaa cagtacattg ttttccaggc tgatgtgatc catcaggtca tcaatcagtt
     3001 cattaatacc gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt
     3061 tattggtggt gtgtttaatc acttccagca gttcgcccgc ttcatcgtgc tcgctttcca
     3121 tcacgctgat tggtcccatt gcctggctgc ccatgccttg tttgatcatc gggaagagga
     3181 tctgctcttc tttcatcatg tggctggata gctcttcatg cagcatagtc aggtattttg
     3241 tcagcccttt tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca
     3301 ggatcagctc cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcaa
     3361 tgatttctgc cagcggggcg ctacgccagt ctttctcaat cggttgttca gcaagctttg
     3421 ccagttcagc ttcaatgacc tcaacatcca gttctttacg tgccgccgcg cgcgccagcg
     3481 tttgcttacc gccacagcag taatccatat catatttacg aaacagagct gaagcgcgag
     3541 gaatagagag cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat
     3601 tcttaataat ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg
     3661 tagcagaggc ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa
     3721 aggtttttct ggttttgctc tgaccgcaat gaccacgcca atcactaaca gcgcgatacc
     3781 gctcagcgtc attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc
     3841 cagcgtttcg aaaacaatca gcggcccgag aatcactgtc ggtaatcgct ggctggcgac
     3901 gttccagcag agtgcgccaa cccatgagca aagcacggct atcgcaacca tcagactaat
     3961 aaacaccagc ggacgggggc caaaaggtaa ggagaagtcc ggcgtttgta tattcagcca
     4021 gtaacaggcg acgagatagc cgatgagaga aaccggcagt gtgaccagcg cctgcgccgt
     4081 tgcccacatc atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc
     4141 ataccacgcc cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc
     4201 ccagtcaaaa tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat
     4261 gccaatacaa accagtgccg gggcgagttt tccccatgcg agtttgccgt cgcgctggct
     4321 ataaagcaga ttggcaaaaa cgggaatcac caccggcagg gtgccgataa tcatcgtgga
     4381 aacaggcgcg ccagtacgtt gaatggcact ggcaaggcag aagtaataga taaggttgcc
     4441 catcatggtg agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac
     4501 gcgtcccagc caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga
     4561 ctgcaacatc gccgggtatt ccggtacgat caacggcccg acaaaaataa gcccccacat
     4621 caaccctgct aacagggcgt acagcacgcc gctaatcatt actggcatcc attggtctgt
     4681 cagaagaaag ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg
     4741 atgcgggagg atgccacttt aattaactag ttattaacat taaaaagatg gcttacgctt
     4801 tcggctaacg ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gttatcaaac
     4861 agaccacctt tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc
     4921 agtccgacgc ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc
     4981 tgtttgccgc tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg
     5041 cccgccagtt cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct
     5101 gccgcataat ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc accgataaat
     5161 acgccgtgtt ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta ccagccgttg
     5221 cgcagcaggg tgtaaacgat gccagaatca gccagcattt tctccgtctc aatgtgctca
     5281 tcggcgaggc cgagcgggga ggtatctgca tgtagcaggc tggtataagc gataaatttc
     5341 acgccagccg cctttgcggc attaataaca ttacgatgct gcggggcacg ttgacccact
     5401 tcgctggaag agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct
     5461 tcatcgccgt agtcagcctg acgcacggta atgccttgtg ctgctagagc ctgggctttt
     5521 gccggattac gaacgatagc cactatttgg ctggcaggaa ccgttttcat caaggattca
     5581 ataatatagt gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat
     5641 cgttttgtct tgtgtttaaa tacgctaaca cctaaactta cttttagtaa gtacgtacaa
     5701 aaaggtaagt atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc
     5761 tttgcggaac agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg
     5821 ttgattctgg tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatc
     5881 ggcggggtga gcgaaaagat gcttgcgcag tcgttacagg cgttagaaca ggatggtttt
     5941 cttaaccgta tcgcgtatcc ggtggtgcca ccgcatgttg aatacagcct cacgccgctg
     6001 ggcgagcagg tgagcgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc
     6061 gaggtgttgg cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg gcctacggtg
     6121 tatgacttgt agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc
     6181 gattgatgat ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat
     6241 cgcaaacccg atatcatcgg tcgcgacttt attcatcgga tactgccctt tctctttaat
     6301 aaacgctgcg gctttatttg acggggaagt ttcgaaacgg atatccagtt tcttatcggc
     6361 agctatcggt gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt
     6421 cgactcatca gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc
     6481 gatatggctg tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc
     6541 aacgaggaac atggcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct
     6601 ttccgcattg gtattaatca tctggcactc gccgtcataa cgggcgggct gggtaacatc
     6661 aatctgataa ttcacaccat caataacatc aaagttataa gtgcggaaac catcccagtt
     6721 gatgagtgac tgtggtttcg tgctgttagg atcaatctgg ttaaactgtc cggcggagca
     6781 ctccagccac tctttcacct ctttaccgct ggctttcact acaatcagcg tattggggta
     6841 gagataaaga tcggcggcat tacggaaggt caactggcct ttttccacct ccacatagct
     6901 tgccgggtca tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag
     6961 ttttgccaga tccggatcgc cctgaatgta atgttcgaca tacgcttttt gcgcgttgtt
     7021 cacgacttgc acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc
     7081 agatttaccg attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt
     7141 ttctaccagc ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt cgtaaatcgg
     7201 ccgagcttcc gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa
     7261 gtcgaccacc ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag
     7321 cgtgcctttg gtgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc
     7381 gtgagcatga ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga
     7441 gttttccgcc atcactttat acggatcggc agacagcccg gaatgtgcca gaacgacaac
     7501 gacatcggca cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc
     7561 attcaccgtc actttcccgg ataaattagc tttatcccag cccatgattt gcggcggcac
     7621 gacgccaata tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt
     7681 atctttaatt aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc
     7741 atttacataa gggaatttcg ctcccgccag cgcatttttc aggtaatcca gaccgtagtt
     7801 aaattcatga ttgccgagtg taccgaccgt ataatccagc gtattcagcg ccttataaac
     7861 cggatgaaca tcacctgttt ttaatccttt cgccgacatg taatctgcca gcggactccc
     7921 ctgaatcaaa tcgccgttat cgaccagtac gctgtttttc acttcattgc gggcatcgtt
     7981 aatcaggctt gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc
     8041 gaaatccatc atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc
     8101 tgcattcaca ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag
     8161 ggacatcctt ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc ttacttatag
     8221 aacagtgaag aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc
     8281 attcattaac gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt
     8341 ggagaaatgt tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag
     8401 gtctacgacg ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg
     8461 gcagcggata ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat
     8521 attccggtcc tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt
     8581 tactggctgg tagacccgct ggatggtact aaagagttta ttaaacgtaa tggcgaattc
     8641 accgttaaca ttgcgctcat tgaccatggc aaaccgattt taggtgtggt gtatgcgccg
     8701 gtaatgaacg taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg
     8761 cgcaagcaga ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat
     8821 gcagatgcgg agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc
     8881 ggctcttcgc tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc
     8941 ggaccaacga atatttggga caccgccgct ggacatgctg tggctgcagc tgccggagcg
     9001 cacgttcacg actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat
     9061 ccggggttca gagtgtctat ttactaaatt cagatggcag aaacagtgta tttcctgatt
     9121 ctgccatc
//
LOCUS       DAZRUF010000163         9073 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZRUF010000163 DAZRUF010000000
VERSION     DAZRUF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.163"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(505..1272)
                     /gene="fecE"
                     /locus_tag="ACHXOL_003083"
     CDS             complement(505..1272)
                     /gene="fecE"
                     /locus_tag="ACHXOL_003083"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR6810007.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1273..2229)
                     /gene="fecD"
                     /locus_tag="ACHXOL_003084"
     CDS             complement(1273..2229)
                     /gene="fecD"
                     /locus_tag="ACHXOL_003084"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR6810008.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2226..3224)
                     /gene="fecC"
                     /locus_tag="ACHXOL_003085"
     CDS             complement(2226..3224)
                     /gene="fecC"
                     /locus_tag="ACHXOL_003085"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR6810009.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3221..4123)
                     /gene="fecB"
                     /locus_tag="ACHXOL_003086"
     CDS             complement(3221..4123)
                     /gene="fecB"
                     /locus_tag="ACHXOL_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR6810010.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4168..6492)
                     /gene="fecA"
                     /locus_tag="ACHXOL_003087"
     CDS             complement(4168..6492)
                     /gene="fecA"
                     /locus_tag="ACHXOL_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR6810011.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6579..7532)
                     /gene="fecR"
                     /locus_tag="ACHXOL_003088"
     CDS             complement(6579..7532)
                     /gene="fecR"
                     /locus_tag="ACHXOL_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR6810012.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7529..8050)
                     /gene="fecI"
                     /locus_tag="ACHXOL_003089"
     CDS             complement(7529..8050)
                     /gene="fecI"
                     /locus_tag="ACHXOL_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR6810013.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8187..8255
                     /gene="ykiE"
                     /locus_tag="ACHXOL_003090"
     CDS             8187..8255
                     /gene="ykiE"
                     /locus_tag="ACHXOL_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR6810014.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            8342..9039
                     /locus_tag="ACHXOL_003091"
                     /pseudo
     CDS             8342..9039
                     /locus_tag="ACHXOL_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
BASE COUNT         1987 a         2510 c         2585 g         1991 t
ORIGIN      
        1 actttttaga aaaatcgatg gtcagaaact ggattagcaa ttccgttcca gggttgcttt
       61 tgatttacgt tggcgtctga tcattgattt atcctcaaaa gcccaacctc attggtaatg
      121 aaccagctcc gtgaatgtcc gctctggcac agagcgaaat tttttgatct cccccccctg
      181 aaatctaaac ttagtcatgt cacgtttttg ggtttctaaa attttaactt cgcgtttttc
      241 gttgccgtaa gggttataca gaaatgtccg ttaagcagag ttcaaaattg attgccgtga
      301 tcacgactgg tttgaaagcc gcgcccaagc ctgtacagct ctggtttgcg ttgattatga
      361 acctgtcagc ctaaagcaag cggatggacg atgagtattg gtaatctttc agagtccgga
      421 aaagttcagc cccagtctga acaggcttgc tggcgccagt ccagtttcat tcagtcgtgg
      481 tttggttctt acggcctgtg caatctacct cattaggcac atcggcctgc cagataccgg
      541 ctcggggtgt atttccgctt ccacgctgaa tactgttctc agcaatcctg gggtcatcac
      601 ctcttctggt gtgccttgcg ccataacatg tccgtttgcc attaccacca gttgatcgca
      661 gtaccggcta gcctgattaa ggtcgtgcag cacagcgacc accgttttcc cctgagtccg
      721 gagttcgccc atcaaccgca tcaggtccac ctggtgattg atatcaagat aggtggttgg
      781 ctcatcaagt aatacaacgg gcgtattctg ggccaggacc attgccagaa atgcgcgctg
      841 gcgctgaccg ccggaaagct cggttaaccg acgaacggca agatgattga tccgggtctg
      901 gttcatggcg acattaactc gtgcattgtc ttcagcggag agacgccccc agagtgacag
      961 ccagggatta cgaccatacg aaaccagctc ctggactgtg atcccctctg gcgttaaatg
     1021 gtgctgaggc agcagcgaaa gcctgcgggc caactggcgc gatgagagca tatttatggg
     1081 attatcgccg agaaatacgg tgccagactg cggcattaaa agccgcgaaa aacagtttaa
     1141 cagcgtcgat ttcccgcaac cgttaggacc gatcagggcg gtgatcttcc ccgttggcag
     1201 tgagagtgaa acgtcgttaa gtaccttgtc tgtcccgtaa ttgaccgtca gattttcagt
     1261 tcgtaaagtc atttatcgca ttctcacaag caaccagaca aaccacggcg caccgataat
     1321 ggcggtcagc acgccaaccg ggagctccag tgggggatga ataattctcg ccagcagatc
     1381 ggcaaccacc aacagcaacg cacctgtcag ggccgaaaca ggcagcagtc tgcggtgacg
     1441 tccaccggtg atgctacgca tcatatgcgg caccacgaga ccaataaagc taatcgggcc
     1501 gcaggcggcc acgccggtag atgtcatggc gacagctagt aacaaagccc agaatcgggt
     1561 atagggcacc gacacaccga gcgtggtggc gcgcgcatcg ccgagtgcaa ggaggtcgag
     1621 atcgcggcaa aaactcaggc tcagcggcag aaataaaatc atcagcggga tggcaatctt
     1681 cacaaagctc cagtcacggc cccataagct gccggtcagc cacagcaggg cgttgttcac
     1741 atcctgcggg cgcgagagca tcagataatc cgtcaggctg gcccagcatg cagaaagcgc
     1801 cacgccggtg agtgccagct tcatcggctg gtgggtcttt gccagcatct tcagcaatat
     1861 caaccccgcc atgccgcccg caaaggccag cagcggcagc accatcacgg gcagtgacgg
     1921 cataagaagt agagccccca cagaggccag gctggcggca tggttaacgc cgagaatatc
     1981 cggtgatgcc agagggttgc gcacaatccc ctgtatcagc acgcccgcca cggcgagggc
     2041 tgcaccgaca aacagtgcca gcagcaagcg cggcagtcgg tactccatca atacataata
     2101 atgctcgcgt ccggcctgcc agtcggtcag cagcgcgcgc cacggcacgg ggatcactcc
     2161 catatggagt gataacagcg cacagcccgc cagggcaagg gtgatgaaaa taaccagcgc
     2221 aattttcatc ctcgcctcct cacaagccag acaaagcaag ggctgccaat cagcgccagc
     2281 actgcgcctg cgggcagatc tccggggaag gccagcgcgc gtgcgagtac atctgccagc
     2341 agcatcagcg tggcccccag cagcatgctc actggcagta cgttgcgctg atcgaagcct
     2401 gcccagaagc gcgccagatg tggcaccagc agaccgataa acgccaccgg acctgccacg
     2461 ctgacgcacg caccaaccag aagcagcact aacatattga tgaccaaacg tagcctcgtc
     2521 aggttcactc ccagcgtatg ggcggtgctg tcgctgaggt tgagcaggtt cagttgattc
     2581 gccagcagca acacgacagg gactgcagtg accaccaccg gcaagagctg ccagacatcc
     2641 tgccagcggg cgtgggacac tccgccagcc agccagtaaa agatgccgta agcatgatct
     2701 tcggccagca gcagggtgat gcgggtaagg cccatacaaa aggccgacag cgcgataccc
     2761 gcgaggatca gtttgtttct gtcatgggta tgacgaaatc cgcctcctgc ggtcatgacc
     2821 agcagccagc tcacgccgcc cccgcatgcc gcgatgaacg acagagaata gcctgcaatc
     2881 ggcgtcggac tcagcgcgct ggtaagcgcc atagccagcg ccgcgccgct gttaatgccg
     2941 agcagtgaag gagaggccat tgggttgtgg gtcagggttt gcagcagcgt gcccgcgagc
     3001 gccaggcttg cgccgatcag aacggcgacc aggcttcgtg gcaaacgaag gttttgcacc
     3061 agcgcttctg gtagcgttgg cgtgtgtcca ggcagcaggg cgcgggttgc atctgctccg
     3121 gaaacaggaa tggccgagta gcaaaacaga ctcagccaga aaataataat aagtgctgca
     3181 acgggaagcc cccacagcag caccgggtgt tttatcgcgg tcatttcaca acggtaagcg
     3241 gctgatggtg gaagattttt accgtgtcag cggcaatacg ctctgcagca aaaataccgc
     3301 gcatccgcgc ccaggtgtta ctgtcgaccg aagcaacctg ctgcttctgc gcggcggtta
     3361 acatctgcca gagcggatct tgttgccagc gtttaacaat gctctcttcg cgatagtggg
     3421 caaccagcag ccaggcagga ttgaccgcca gcagttgctc caggccgatg gacggcatgg
     3481 acgcacccgc catcgcagcg ggaacgttca gccccagaga ggccagcacg ctgccggtcc
     3541 aggtctcctg agtatgcagg ttgaattgct gttcgcgtga tgtgccaaag gccacgcgtg
     3601 tccctttggg aagctggctg gcccactgcg ccatcctctc tttatgttgt tccagacgtg
     3661 cctgcatctc tcgcttttta cccaccattt cgccgatgat agccgcagat tgcaaatttt
     3721 cagcgtaggt ttcgttgcgg gacttaagca gcagtaccga cgcgatttgc tgcaaggcga
     3781 tgtaaacccc cgcatggcga ctgctgtcgg caatgatcag gtctggtttc agagcggcaa
     3841 tggcttccag gctcggctgc gcgcgcgttc cgacggactg ccacggtttc aggtgcgcac
     3901 gcacttcggg caggatgcgt tttgcatcgt tatcgtcggc aataccgatc gggctgacgt
     3961 ccacggcggc cagcgcatcg gcgaacgaga gttccagcac cacaatccgt tgtggcgttt
     4021 tttcgagtgt aaacgtgccg tgttcgtcct gaaccgtggc ggcaaaggcg tggctgatca
     4081 ccagcagcag gcctgcaaaa agaaaacgga taaatgccaa cataatcaca ttccagctaa
     4141 aagcccggca agccgggcgt taacacatca gaacttcaac gccccctgca tatacagcgt
     4201 gcgcggctgg cctgcataga tgcctttgtt gttgtcgtca taagagcgga tgaagtagtc
     4261 ctggtcgaag atgtttttca caccgaatgc caggttcaga tctgccatct gcgggccaaa
     4321 gtcatacacc acgcgtgcgc cccagagcat gaagccggga atgcggccgg tactgccgtc
     4381 ggcgctctct ttcaccgtat tggcgttatc cgcaaactgg ctggactgga aatcgctgtt
     4441 cagattgaac atccagtttc ctggcttgta gtccacgccc agcgtgcctt tatgtttcgg
     4501 ggagaatggt accagattgc cgtaggtgtc gcctttctcg cggatttccg cgttcacata
     4561 cgcatagctg gcgtagatgg aaacgttatc aagcgttggc gttagcgtac ccagatcgta
     4621 acgtgcctgc gtttccagcc cggtatggcg cgttttgcca cgtgcagtga cggtgtcgtt
     4681 ggtctggttg gagtcgtact gattgttaaa gttaatcagg aacagcccca tttccgccgt
     4741 cagcgcgccg tcgtcgtagc gggtaccgag ttcccaggtt cgcgcttttt ccggttccac
     4801 attgccgctt tgcacagcct tgccaatctg gctgtactgt acggtgccga acgagccttc
     4861 agtgtttgca taaagattcc agctgtcagt caggtgatag agcacgttca acgccggaag
     4921 cggtgcgtta tagctcactt cttcgtgcgt gcctgtgatg gcgttgttct ggtatgactc
     4981 gatatgttcg aaacgcatac ccggcgtgat ggtccagttg ccgatgtcga ttttgtcatc
     5041 cagataccag gcgtgcgcct cggtgccgga acgcgtatcg cggtcgtaag ggcttgagcc
     5101 ggacggcaac tgcccgctgc tggtggcggt gtagtaacgc atttcatgcg ttgattcatt
     5161 cacatagcga tagcccacgc ccacttcgtg cgcggaaggg ccgatcataa agatctggct
     5221 gtagcgtggc tcaataccgc gcacccagta gttacgcggc gagagggtga tgcgtttgcc
     5281 ttgctccagg tagccgctgc gcagggtttg ggtgtagaac ccctgaatgt tgaatttatg
     5341 ctggctgtct ggctggaact ggtagcccag gctcgccagc ttgcgacgac cccagaagcg
     5401 gtcatacggg cgggtggatt gccagcgatc ggcgtcgtaa tccgcgcgag acaggccacc
     5461 gggcatgtcg gcttcaccgt cgtaatattg cagcaggctg ttgaaggtgt gcacctcatc
     5521 cggcgcatat ttgcttttca gcatcaggtc gtcgatgcgg gtggcgctgt gctcgcgcca
     5581 gtcactgccg cgcgtgccgg agtagagcag cgcggtgcca aaaccgttgt ccgctgtgcc
     5641 gcccaccatc aggttgtgcg tctctttcgg gttgttttgt gaagaggttg ggctgagctg
     5701 accttccacg cccgcctcga taccaaagtc ctgcggaatg gcacgggtaa caaagttcac
     5761 cacgccgccc acgctctgcg gtccgtaacg caccgcacca ccaccgcgta ccacgtcaat
     5821 ggcatccatg ttgccgagcg aaacgggagc cagtgaaagc tgcggctgac cgtaaggggc
     5881 gaaggggacg gggatgccgt ccatcaggac ggtcgagcgg ctggcgaggc gcgggttcag
     5941 gccccggatg ccaaagttca tcgccaggtc gtggctgccg gtgccgttgt tttccggcgc
     6001 gctgacgcca gggatgcggt taagtacctc acgcatggtg gttgcgccgg ttttggcgaa
     6061 atcctcacgg cggatcacgt cacgcgcgcc agcatgttca aatacgtcgt tttcacgcgc
     6121 atcacccagc cagtcgccga ccacggtcag ggcatcttct tttggtgcgg gcgcgggctc
     6181 cagcgtccag ctgttatttc ccagcggttt tacctgcagt ccgctgccgt ccagcagttg
     6241 ttgcaggccg ctctcgacgt cgtaatcgcc gtgcaggccg ttgctctgct tgccgcgcgt
     6301 caggctggcg tcaaccgaga gggtaaatcc gctgtgtgcg gcatactgat tgagcgcttt
     6361 atcgagcgat cccggtgcga tattaacctg tgcagcaaaa gcggaaaacg agagaccggc
     6421 cagcggcagc aggctcaggc gaatggcgtt aaccaaaggt gttgttttac gaaaaacgcg
     6481 taacggcgtc ataccttccc catcatcatt tttgttgtgt tcagctatga gtcgaacgag
     6541 aagggtaaaa aggacaatcg aaataagaat tattttcctt acagtggtga aatgtttatc
     6601 cagtaccgcg taatagactg aattttaacg ggaagcgttt gcgcgataac gttcaggatc
     6661 gcatcggtat ttttcagcgg gaacgtcccg ctcaggcgca gcccggcaac ggcgggatcg
     6721 cagcgcagca cgccgttgcg gtaacgggtt agcgtggcta tcacctcacc cagcggttta
     6781 tcgctgaagc tcaggatgtc cttcgtccag cttgtactct cgtcatccag cggtttcact
     6841 gcgccaaact cagaggcgct gaactgcagg ctttcaccag cgttcacgat gcgtttttgc
     6901 gcgggggcac tggcgagaag cacttccacg gcgtgctgct gcacgtcaag ctgcgtgaaa
     6961 ttatcctgct ggcggacggt aaattctgtc cctaaagcag tgagctggcc ctgacgggtc
     7021 aggacacgga aggggcgttg cagggcatct ttcgcggtag taatggcgat ttcaccgtac
     7081 cagagccgga cggtgcgctg atgcgcatca aaacgcatat ccgccgcgct ttgggtattc
     7141 agcgtgagca gggagccatc ttccagttgc tgacggctga ctgtgccttt ggcggtgcgg
     7201 taatctgccc gcagaccttc gccggtttcc gactgccaga gctgccagcc tccgccagcg
     7261 ccgagcaaca gcagtaatcc tttcatcacg tgacggcggg tgaggcgggt atcgtgcaac
     7321 gcccggctgg caacgtcgcc aggcacaccg ccaagctggt tgcgcaggtt ttcaacctgc
     7381 tgccaggccc actggttatc ctgatcctgt tcataccact gttgccagcg cgcttcctgt
     7441 tgtgggctga cgcgctcgcc gcttagcacg gcataccagt gggaagctga acgcagcgcc
     7501 tgacggcggg aatcggttaa caaaggattc ataacccata ctccagacgg aacagcaggc
     7561 agtgctcgac ggctttcgcc acgtattttt tcacggagct gatggaaaca ccgagtttgt
     7621 gcgcaatctc gctgtatgtc agaccatcca gttgcgaaag cagaaacgct tcacgtgttt
     7681 tgccgtttag cccgtccagc atgctgtcga ggagttgtag ggtctcgagt tggctttcgc
     7741 gttcctcagg tgaaggcgct cccccctccg gcataagcgc cagcatctcc agatacgctt
     7801 tttccagcgc gtttcggcga aacaggtcca ccatcacgcg tttggcgata gtgcagagga
     7861 aggagcgagg atcgcggatc gtcgagagcg tttcgctgac cattacccgc aaaaaagtgt
     7921 cctgggcaat gtcatctgca tcaaaagcag actggagttt gcgcgtcagc cagcttttca
     7981 accagccgtg atgtgtgcca taaagcgact cgaatgttaa ggaagctgtg gtagtggcgc
     8041 ggtcagacat gcggagtgca tcaaaagtta attatcacgt agccatatta atatgagaat
     8101 agttatcatt acaattggaa ataaaattgt ttccaataga catttttaac atgttgtttt
     8161 tctaagtgtt ataaggtagg tataaaatgg gatggagcct ctgcttctgg catgtgtcgg
     8221 tcagaatgac tcatgatgtg gtctgctatt attgacatcc tcactgccct aaaggatagg
     8281 gatttcggta atgctgtcaa cttactgatt tagtgtatga tggtgatttt aaggtgcttg
     8341 cgtggcttcc atttccatca gatgtccttc ctgctccgct actgaaggcg tggtgcgtaa
     8401 cggcaaaagc actgccggac atcagcgcta tctctgctct cattgccgta aaacatggca
     8461 actacagttc acttacaccg cctctcagcc cggtaagcac cagaaaatca ttgatatggc
     8521 catgaatggc gtcggatgtc gcgccagtgc acgcattatg ggcgttggcc tcaacacggt
     8581 tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca accgggcagt
     8641 gatgtgattg tctgcgctga aatggacgaa cagtggggct acgtcggtgc taaatcacgt
     8701 cagcgctggc tgttttacgc gtatgacagg atacggagga cggttgtggc gcacgtcttc
     8761 ggtgaacgca ctctggccac actggagcgt cttctgagcc tgctgtcggc ctttgaggtc
     8821 gtggtataga tgacggatgg ctggccgctg tatgaatcac gcctgaaggg aaagctgcac
     8881 gttatcagca agcgttacac tcagcgcatt gagcgacata atctgaatct gagacaacat
     8941 ctggcaaggc tgggacggaa gtcactgtcg ttctcaaaat cggtggagct gcatgacaag
     9001 gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat tacctcctcc
     9061 cgaactcaaa act
//
LOCUS       DAZRUF010000164         9001 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZRUF010000164 DAZRUF010000000
VERSION     DAZRUF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.164"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            71..1606
                     /gene="lsrA"
                     /locus_tag="ACHXOL_003092"
                     /pseudo
     CDS             71..1606
                     /gene="lsrA"
                     /locus_tag="ACHXOL_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1600..1776
                     /locus_tag="ACHXOL_003093"
                     /pseudo
     CDS             1600..1776
                     /locus_tag="ACHXOL_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(1784..1873)
                     /locus_tag="ACHXOL_003094"
                     /pseudo
     CDS             complement(1784..1873)
                     /locus_tag="ACHXOL_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2080..3528)
                     /gene="uxaB"
                     /locus_tag="ACHXOL_003095"
     CDS             complement(2080..3528)
                     /gene="uxaB"
                     /locus_tag="ACHXOL_003095"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR6810015.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3755..4705)
                     /locus_tag="ACHXOL_003096"
     CDS             complement(3755..4705)
                     /locus_tag="ACHXOL_003096"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR6810016.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4844..5203)
                     /locus_tag="ACHXOL_003097"
     CDS             complement(4844..5203)
                     /locus_tag="ACHXOL_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR6810017.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(5203..6129)
                     /gene="glsB"
                     /locus_tag="ACHXOL_003098"
     CDS             complement(5203..6129)
                     /gene="glsB"
                     /locus_tag="ACHXOL_003098"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR6810018.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(6193..7581)
                     /gene="sad"
                     /locus_tag="ACHXOL_003099"
     CDS             complement(6193..7581)
                     /gene="sad"
                     /locus_tag="ACHXOL_003099"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR6810019.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            7682..8563
                     /locus_tag="ACHXOL_003100"
     CDS             7682..8563
                     /locus_tag="ACHXOL_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR6810020.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            8640..>9001
                     /gene="yneK"
                     /locus_tag="ACHXOL_003101"
     CDS             8640..>9001
                     /gene="yneK"
                     /locus_tag="ACHXOL_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR6810021.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRH"
BASE COUNT         2321 a         2353 c         2145 g         2182 t
ORIGIN      
        1 aggttatgaa caaattaaaa gcagaaatac atttgttcaa aactcacctg caaaactgaa
       61 cgggggaaat atgcaaacga gtgatacccg cgcgttaccg ctactttgcg cccgctcggt
      121 ttataaacag tattcagggg tcaatgtcct gaaaggcatc gattttacgt tgcatcaagg
      181 ggaggtccac gccctgctcg gcggcaatgg tgccggtaaa tcgacgttaa tgaagattat
      241 tgccggtatt acccctgctg atagcggtac gctggagatt gggggcaaca actacgtcag
      301 attaacgcca gttcatgctc atcagctggg tatttatctc gttccccagg aaccgctgct
      361 tttcccaagc ctgtcgataa aagaaaacat cctgtttggg ctggcaaaaa aacagctctc
      421 catgcagaaa atgaagaact tgctggcggc gctgggctgc tagtttgatc tgcatagtct
      481 ggcaggatcg ctggatgtcg ccgatcgcca aatggtggaa atcctccgcg gcctgatgcg
      541 cgactcgcgg attctgatcc tcgatgaacc taccgcctcg cttacccctg cggaaaccga
      601 acgcttgttt cgtcgcttgc aagagctgct tgctactggc gtgggtattg tttttatctc
      661 gcataagctg ccggaaattc gccagattgc cgatcgaatt agcgtgatgc gcgacggaac
      721 catcgcctta agcggcaaaa ccagcgaact gtctaccgat gacattattc aggccatcac
      781 gccagtggta cgggaaaaat cgctctctgc cagccaaaaa ttatggctgg agttacctgg
      841 caaccgccca caacatgccg ccggaacgtc ggtgctgaca ctagaaaatc tgaccggcga
      901 aggtttcagg aatgtcagcc tgacgctcaa tgccggagaa attctgggtc tggccgggct
      961 ggtgggggcc ggacgcacag aactggccga gacgctctat ggtctgcgta ctttgcgtgg
     1021 cggacgcatt atgctgaatg gtaaagagat caataaatta tccactggag aacgtttact
     1081 gcgcggtctg gtttatctgc cggaagatcg ccagtcatcc ggactgaatc tcgatgcttc
     1141 gctggcctag aacgtctgcg cccttactca taaccttcgt ggattctggg cgaaaaccgc
     1201 gaaagataat gccaccctgg aacgttatcg tcgggcgctg aatattaaat ttaaccaacc
     1261 ggaacaagct gcacggacat tatccggtgg caaccagcaa aaaatcctga ttgccaaatg
     1321 cctggaagct tcgccgcaag cattgattgt cgatgagccg acgcgcggcg tggatgtctc
     1381 ggcccgtaat gatatctacc agctgttgcg cagcatcgcc gcacagaatg tggcagtgct
     1441 gcttatctcc tctgatctgg aagagatcga actgatggca gatcgtgtgt atgtgatgca
     1501 tcagggcgaa attacccact ctgcactgac cgggcgcgat attaatgtcg agactattat
     1561 gcgcgttgcc ttcggcgata gtcagcgtca ggaggcgtca tgctgaagtt tattcagaac
     1621 aaccgtgaaa tcacggcact gctggcggtg gtgctgctgt ttgtattacc cggttttctc
     1681 gaccgccagt atttaagtgt gcaaacgctg accatggttt atagcagcgc gcaaatcctg
     1741 atcctgctgg caatgggcgc gacgctggta atgcttatgc gcaaagtagt aagcgcgatg
     1801 atatcttgga taatactgag ccgtgccaga caaaaatacg gcgcagccag tgttgttgtg
     1861 gacgaacaat catggttatc ttcaggcgtg aaaaagtcgc cctattaaac gtgattacga
     1921 tcacattctc aagacgctac ttacaaatta cctacaagct tacaacatta acaggcgctg
     1981 catgtggcat ctgttaatga taaaggcaca taggatgttg taactaatat agtgaaataa
     2041 gaaacccggt caaaaccggg ttcagaagta acggtgttat tagcacaacg gacgtacagc
     2101 ttcgcgcatc cctttttcaa gaatcgcatc caggtcatta gcaacctgct cgaccagacc
     2161 aggcacttgc gtcaggtcct gctcccagtg gtctttttct gccaacacaa tcgctaccag
     2221 ttcctgggta ccgataacat ggtcacgatg ctggctccag agttgctgat aacgttccag
     2281 ccagtgtgca tcgtcctgta ccggatatgt ttctccgtta cgctcaccac gatagaacgc
     2341 aatcaatgcc gctaatgcga aagtaaggcg cgccggaagt gtgccgtttg ccttctgccc
     2401 tgccagcagc tgcggcagga tgcgggtgcg gaacttggtc ataccgttga gcgcgataga
     2461 cagcagctga tgcttatacg ggttacggaa acgcccggtg actgcactgg cgaaagattc
     2521 cagttcatca cgaggcaaat ccagtaccgg aataatttct tcgtagatgg ctttttcaac
     2581 gaatgcgcaa atttcagcat cgttcatcgc ttcacctacg gtatccagcc ctgcctggaa
     2641 cgccaccggc accagcgcgg tgtgcgcacc gttgaggatt gccactttgc gttctttata
     2701 cggtttaata tcgtcaacaa tcagcacgtt gagcggatat ttgtccaggc gcagctcagt
     2761 cgctaaggat ttcggcccct gaatcacaaa caggtaaaag tgttcagcgg tgtcgaggaa
     2821 accatcgtga taacccagtt cttcttccag tttagccact tcatcgcgcg gataaccggt
     2881 aacgatacgg tccaccagcg tagaacagaa gctgttagcc tgatccagcc attgaataaa
     2941 tgcttctggc agtgcccact cttgcgcata gcgcagcacc agttcacgca acgcgtcgcc
     3001 attgtagtcg atcaactcac acgggatgat gatccaacct ttatccagcg caccgttgaa
     3061 atggctaaag cgttcgaaca gcagacgggt cagttttgcc ggatagctta tcgctggcgc
     3121 gtcatcgaat ttatcgcccg catggtagct gatccccgct tcggtggtgt tagagaaaac
     3181 aaagcgcatt tccgggttgt gcgccagttt caggaattca tcgtattcac tgtagacgct
     3241 gatttcacga ttaaccgagc gaatcagacg cgcgtcgctg accgcttccc ccttctcatt
     3301 caggccacgg ataatggtgg tatacagacc atcctgcgtg ctcagtgacg gcgggaatga
     3361 agtttcaatc ggacgaacaa cgaccacgcc agaattcaga tcggtgtgct cattcaggag
     3421 atcgatttgc cagtcaacaa aggcgcgcag gaagttacct tcaccaaact gaatgatacg
     3481 ttctggatac tgtgcaccgg gaaaatcgcg acgatttagt gttttcacaa tgggttccct
     3541 tctgattagt catacaacct gtttgaattg gtacgacagg ttagcaaact ttaatacgcc
     3601 gaacccctgt tttgatcaac tcctgatgat taatgagcag ttttatgaga aaagtgtggc
     3661 gcggatcatg gtttaatcga ggaaatacgc cttttcctgg atcataaagt ggtagaacac
     3721 attgcattca aatcgcgcgg aatgaataaa gatgtcagac aacttcctca ccgtaacgca
     3781 tagtgctggt acggttgcgc ccatctttct tcgagcgata cagacacgta tcggcttcga
     3841 ccatcaattt attaaaatca tcggtcagcg ttcggtacga tgcgcgacca ctaccgacgc
     3901 caatacttac cgtgagataa agcgtttttt gttgccaggt gaatggttgc agttcaacgc
     3961 ctttacggat tttttccgcc attagcagac catcgacagg attcaccgac ggcactgcaa
     4021 cagcaaattc ttcgccgccc atgcgcgcca ccagcccctt atcaccgaca atcttctgaa
     4081 tatgccgggc aaacacgctt aacactttat cgccacattc atgtccatag ttatcgttaa
     4141 tgcttttgaa atagtcgata tcaagcagca tgaccgtcag aggcacgatc aacacgcccg
     4201 ttgagaattg ctcactgaac cagtccgcaa gcaaaggcca gaaatccaga ctatcaatac
     4261 tgaccgaacc aatcgccccg acaatagcgc ataataatgc ggcaatcaga cagtaattaa
     4321 atagccgtaa ggcacttact ggctcatact tatttttccc cagacgctta tcccgcacga
     4381 ccagtaaggc gacggtaaca ataaacatca tattggagaa attgataacc agtgaaacca
     4441 gtccccattc ggtggttatg gcatcataaa ccagcatcgc cacataactg atcgcatagt
     4501 aatgcagtcg attaagccag acatagcggg caaacccccc cgccattacg ccattcagcg
     4561 gccagaaaag agaaagttcg tgaaccagcc gtaactctgc accaataaaa tagaacagcg
     4621 tggttagcgc aaaaatagcg atgctattac gtagcagatg accttcctgg aacaaacgga
     4681 aagttgaaat ggggtgtaca tgcattaatt atctgtccat acaattgcgg gtggcaatta
     4741 tctcaacgta ataagtaaat tctcataaag gatagcatgt tcgctaacag cggcggcaaa
     4801 taaaggtacc ctgggaatgc tattttatcg ttttccgcaa ttctcatggc tgattcatct
     4861 gtacaaccag ccagtgatag attaccgcca cgatataacg ttgttgttct tcgcttaacg
     4921 aaacaccctg cggaatgtta tgccacttag ccagacaacc gcgacagcag gtcgcggtgg
     4981 cgtgctgagc gataaacacc ggatgcccgc gcatgggggt ttgcttaccg tcattagccg
     5041 gtaatgccgg agcaagacgt ttagcgacaa aatccgccgc atgttgttcg atgactggcg
     5101 cgcctttctc caggcaatac tgacgctctt tcatgcccag acgaaagcga gagcgaaatt
     5161 ttgaacggga taaacgcgcg aagagtggat cgagagactg cattaataaa ccgaacgccc
     5221 taactgtttc gtcaattgtt caaggacggc gatacccgca agcgagttac ctgcatcatc
     5281 cagttccgga ctccagactg cgatggccat ttcatgcgga acaatcgcca caataccgcc
     5341 accaacgcca gatttcgccg gtagtcccac ccgccaggca aactcccccg cgttctggta
     5401 cataccactg gtcgccatca gcgcgttaat ttgccgcgcc tgcattggcg tcactacagg
     5461 ttcatcaata tgaatagctt tcccctgatt agccagaaag acaaacgtcc gagccagctc
     5521 tacacagctc attttcagag cgcagtaatg aaagtagttt tgcagaacgg ttgtcacgtc
     5581 atgatggaaa ttgccaaacg acttcatcag ccaggcgata gccgcatttc gcgcggaatg
     5641 ttcaaattcg gaacgcgcta ccaccgtatc gtatgaaata tcagacacac cgcttaagcc
     5701 gcgcacgact tccagcatac gttgccgtgg tgcgcttaat cgcccttgca acatatcgca
     5761 gaccaccagc gcaccagcat taataaacgg attacgcgga ataccctgtt ccatttccag
     5821 ttgcactaaa gaattgaacg gtgatccaga cggatctttg ccgacgcgtt gccagatttc
     5881 ctcttcggag taatgacgca tggcgacaac gagactcagc actttggaaa tagactgaat
     5941 ggaaaaacgt tcttgcgcgt ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc
     6001 aatgcccaat cgggaaccgt ctactgtagc cagcgccgga atataatccg cgactttacc
     6061 ctgaccaatg agcggccgca cttgccgcaa gatgttttct aaaattgcat tatccatggc
     6121 gactgccact ttctactcct ggaccgcagg tctgaaaaga cctgcgagta tatcagagct
     6181 gaatatgtcg cgtcagatcc ggtctttcca caccgtctgg atattacaga attcgtgtaa
     6241 gccgaaatgg gaaagctcac gaccaaagcc actctttttc acgccaccaa aggccactcg
     6301 cgcgtcgctg gcacaataac cattgataaa caccccaccg cattccagac gtgccgccat
     6361 ctgtctggcc tgtgtttcgt cggtggtaaa aatggtcgct gaaaggccga actcactatc
     6421 attagccagt tccagcgcat gttctgcatc tttcgcaacg gtgattgccg caacggggcc
     6481 aaacatttct tcccgaaacg cggtcatttc tggggtaaca ttcgccagaa ccgttggcgg
     6541 atagtagtta cctgccccag ccatcttttc cccgcccagt aacaaacgcg caccctgcgc
     6601 cagggttttc tctacctgat gatgcagctc atcacgtaaa tcaaaacgag ccattggtcc
     6661 gagagtgttc tcttcgtcac ggggatcgcc cattttcaag gctgccgcag ctgccacaaa
     6721 acgttcggta aatgcagaag caattccctc ttcgataata aagcgttttg ccgctgcaca
     6781 tacctgtccg gtattctgat aacgtccggc taccgccgct ttaaccgcca gttccagatc
     6841 ggcatcgtta agcacaataa acggatccga accgcccagt tccagtacgc attttttcag
     6901 tgccgctcca gcctgtgcgc caatagccgc tccagcacga acacttccgg tcaccgtgac
     6961 agcagcaatg cgcgagtctt taatcatctg actgacaccg tcgttgtcgg cattcagcca
     7021 gccatatacg ccttgcggga ttcccgcatc tttaaacacc tgggcaatga gctgagcaca
     7081 tcccatcaca ttcggcgcat gtttaagtaa gtagccgtta cctgcaagaa tgatgggaac
     7141 agcgccacgc atcacctgcc ataacggaaa attccacggc ataatcgcca gaatcgtccc
     7201 caacggtcga tactcaataa ccgcctgctg attttccacc agtgtaggtt ccgccttcag
     7261 cattgccgga ccatgttctg cataccagtc acacaaattc gccgatttcg ccacttcagc
     7321 gcgcgcctgg ttgattggtt tgcccatttc gcgggtgatc atttgcgcca tttcttcgct
     7381 acgggcgcgc agagccttac cgatatcacg cattttttca gcacgataat ctatatttgt
     7441 ctcgcgccag tcgcgaaagc ctgctgccgc cagctcaagt gcgttttcga tatcgttagc
     7501 gccagcccac ggcagcacag aaagttgttc acccgtggca ggatttattg aaattgcatg
     7561 agttgccgga gtaatggtca tcggggtatc tcctttatga gtcatggtat gaagatacgc
     7621 agatttactc ttgctttaaa atgaataata ttaagccact tattcacgaa tcgagaatgt
     7681 tatggatctg acccaactgg agatgttcaa cgcggttgcc gaagctggca gcattaccca
     7741 ggctgcagca aaagtgcatc gcgtaccgtc gaatctcact acccgtctac gccagctgga
     7801 aacagaactg ggggtagatc tgtttattcg cgagaatcag cgtttacgtc tctctcctgc
     7861 cgggcataac tttttacgct atagccaaca aattctcgcg ttagtggatg aagcgcggag
     7921 cgttgtcgct ggcgatgagc cgcaaggttt attttctctt ggttcgctgg aaagcaccgc
     7981 agcagtgcgc attccggcca cgctggcgga attcaaccat cgttatccca aaattcagtt
     8041 ttcactttcc accggccctt ccggcactat gctggaagga gtgctggagg gaaaactgaa
     8101 tgcggcattt attgatgggc ccattaacca tactgccatt gacgggatac cggtataccg
     8161 cgaggagctg atgatcgtca cgccacaagg tcatgcgcca gtaatccgcg ccagtcaggt
     8221 taacggcagt aacatttatg ccttccgcac caattgttcg tatcgtcgcc acttcgagag
     8281 ctggtttcat gctgacggtg ccgctccggg aactattcac gagatggagt cgtatcacgg
     8341 gatgttggcc tgtgtggtcg caggagcagg cattgcgctt attccacgct ctatgctgga
     8401 aagtatgccg gggcatcacc aggttgaagc gtggccgtta gctgagcaat ggcgttggtt
     8461 aacaacctgg ctggtctggc gtcgcggtgc gaaaacgcgt ccgcttgagg catttattca
     8521 actactggat gtgtctgact cggcaaaaca gagttaccaa tgaactattt tgatagttct
     8581 tgcggctaat atgctctata tagtgatgtt ccgatgactt atgactatat ggggcaaata
     8641 tggttacgcc agtaagcatc agcaattaca tatctcttcc tgatgatttt cccgttcgta
     8701 atattgcacc acaggtaaaa gaagttttaa aggactttat tgatgcgcta agtacaataa
     8761 tatgtgatga agagtggcgc acgtctttaa acatcaactc agccacaaaa aagatattta
     8821 ataaccttga taacttatca tatattcaga ggacttcttt ccggggtaac gacacgctat
     8881 acaatgaaaa ggttcagttt aaactcactt atcccgtcaa gaatggaaga cacaaagaaa
     8941 atattgaatt tcaggtagta ataaatttaa gccctattta tttagataat tttcgccatg
     9001 a
//
LOCUS       DAZRUF010000165         8979 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZRUF010000165 DAZRUF010000000
VERSION     DAZRUF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.165"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            297..2690
                     /gene="malP"
                     /locus_tag="ACHXOL_003102"
     CDS             297..2690
                     /gene="malP"
                     /locus_tag="ACHXOL_003102"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR6810022.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLVGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2700..4784
                     /gene="malQ"
                     /locus_tag="ACHXOL_003103"
     CDS             2700..4784
                     /gene="malQ"
                     /locus_tag="ACHXOL_003103"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR6810023.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4829..6145)
                     /gene="gntT"
                     /locus_tag="ACHXOL_003104"
     CDS             complement(4829..6145)
                     /gene="gntT"
                     /locus_tag="ACHXOL_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR6810024.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6506..7081)
                     /gene="nfuA"
                     /locus_tag="ACHXOL_003105"
     CDS             complement(6506..7081)
                     /gene="nfuA"
                     /locus_tag="ACHXOL_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR6810025.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7140..7823)
                     /gene="gntX"
                     /locus_tag="ACHXOL_003106"
     CDS             complement(7140..7823)
                     /gene="gntX"
                     /locus_tag="ACHXOL_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR6810026.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7861..8631
                     /gene="bioH"
                     /locus_tag="ACHXOL_003107"
     CDS             7861..8631
                     /gene="bioH"
                     /locus_tag="ACHXOL_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR6810027.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(8660..>8979)
                     /locus_tag="ACHXOL_003108"
     CDS             complement(8660..>8979)
                     /locus_tag="ACHXOL_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR6810028.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         2207 a         2238 c         2495 g         2039 t
ORIGIN      
        1 tattttactt gtgaaactgt ttcactcctt tacttaattt atagagttac cttccgcttt
       61 ttgaaaatac gcaacggcca ttttttgcac ttagatacag attttctgcg ctgtattgca
      121 ttgatttgct gctaatcctg tggtttgcac tagctttaag tggttgagat cacatttcct
      181 tgctcatccc cgcaactcct ccctgcctaa tcccccgcag gatgaggaag gtcaacatcg
      241 agcctggcaa actagcgata acgttgtgtt gaaaatctaa gaaaagtgga actcctatgt
      301 cacaacctat ttttaacgat aagcaatttc aggaagcgct ttcacgtcag tggcagcgtt
      361 atggcttaaa ttctgcggct gaaatgactc ctcgccagtg gtggctagca gtgagtgaag
      421 cactggccga aatgctgcgt gctcagccat tcgccaagcc ggtggcgaat cagcgacatg
      481 ttaactacat ctcaatggag tttttgattg gtcgcctgac gggcaacaac ctgttgaatc
      541 tcggctggta tcaggatgta caggattcgt tgaaggctta tgacatcaat ctgacggacc
      601 tgctggaaga agagatcgac ccggcgctgg gtaacggtgg tctgggacgt ctggcggcgt
      661 gcttcctcga ctcaatggca actgtcggtc agtctgcgac gggttacggt ctgaactatc
      721 aatatggttt gttccgccag tcttttgtcg atggcaaaca ggttgaagcg ccggatgact
      781 ggcatcgcag taactacccg tggttccgcc acaacgaagc actggatgtg caggtaggga
      841 ttggcggtaa agtgacgaaa gacggacgct gggagccgga gtttaccatt accggtcaag
      901 cgtgggatct ccccgttgtc ggctatcgta atggcgtggc gcagccgctg cgtctgtggc
      961 aggcgacgca cgcgcatccg tttgatctga ctaaatttaa cgacggtgat ttcctgcgtg
     1021 ccgaacagca gggcatcaat gcggaaaaac tgaccaaagt tctctatcca aacgacaacc
     1081 atactgccgg taaaaagctg cgcttgatgc agcagtactt ccagtgtgcc tgttcggtag
     1141 cggatatttt gcgtcgccat catctggtgg ggcgtaaact gcacgaactg gcggattacg
     1201 aagttattca gctgaacgat acccacccaa ctatcgcgat tccagaactg ctgcgcgtgc
     1261 tgatcgatga gcaccagatg agctgggatg acgcttgggc cattaccagc aaaactttcg
     1321 cttacaccaa ccataccctg atgccagaag cgctggaacg ctgggatgtg aaactggtga
     1381 aaggcttact gccgcgccat atgcagatta ttaacgaaat taatactcgc tttaaaacgc
     1441 tggtggagaa aacctggccg ggcgatgaaa aagtgtgggc caaactggcg gtggtgcacg
     1501 ataaacaagt gcatatggcg aacctgtgtg tggttggcgg tttcgcggtg aacggtgttg
     1561 cggcgctgca ctcggatctg gtggtgaaag atctgttccc ggaatatcac cagctatggc
     1621 cgaacaaatt ccataacgtc accaacggta ttaccccacg tcgctggatc aaacagtgca
     1681 accctgcact ggcggctctg ttggataaat cactgcaaaa agagtgggct aacgatctcg
     1741 atcagctgat caatctggaa aaattcgctg atgatgcgaa attccgtcag caatatcgcg
     1801 agatcaagca ggcgaataaa gtccgtctgg cggagtttgt gaaagttcgt accggtattg
     1861 agatcaatcc acaggcgatt ttcgatattc agatcaaacg tctgcatgag tacaaacgcc
     1921 agcacctgaa tctgctgcat attctggcgt tgtacaaaga aattcgtgaa aacccgcagg
     1981 ctgatcgcgt accgcgcgtc ttcctcttcg gcgcgaaagc ggcaccgggc tactacctgg
     2041 cgaagaatat tatctttgcg atcaacaaag tggctgacgt gatcaacaac gatccactgg
     2101 ttggcgataa gttgaaggtg gtgttcctgc cggattattg cgtttcggcg gcggaaaaac
     2161 tgatcccggc ggcggatatc tccgaacaaa tttcgactgc aggtaaagaa gcttccggta
     2221 ccggcaatat gaaactggcg ctcaatggtg cgcttactgt cggtacgctg gatggtgcga
     2281 acgttgaaat cgccgagaaa gtcggtgaag aaaatatctt tatttttggt cataccgtgg
     2341 aacaagtgaa ggcaattctg gccaaaggct acgacccggt gaaatggcgg aagaaagata
     2401 aggtgctgga cgcagtattg aaagagctgg aaagcggtaa atacagcgac ggcgataagc
     2461 atgccttcga ccagatgctg cacagtatcg gcaaacaagg cggcgatccg tatctggtga
     2521 tggcggattt cgcagcctat gttgaggcac aaaagcaggt ggatgtgctg taccgcgacc
     2581 aggaggcctg gactcgcgcg gcgatcctca ataccgcccg ctgcggtatg tttagctcgg
     2641 atcgctctat tcgcgattat caggctcgta tctggcaggc aaaacgctaa ggaagctcga
     2701 tggaaagcaa acgtctggat aatgccgcgc tggcggcggg gattagcccc aattacatca
     2761 atgcccacgg taaaccgcag tcgattagcg ccgaaaccaa acggcgtttg cttgacgcga
     2821 tgcatcaacg taccgccacg aaagtggcgg taacgccagt cccgaatgtc atggtttata
     2881 ccagcggcaa aaaaatgccg atggtggtgg agggcagcgg cgaatatagc tggctgctga
     2941 ccaccgaaga aggaacacag tacaaaggcc atgtaacggg gggcaaagcg ttcaatctac
     3001 cgacgaagct gccggaaggt tatcacacgc tgacactcac ccaggacgac cagcgcgcgc
     3061 attgccgggt gattgtcgcc ccgaaacgct gttacgaacc gcaggcgttg ctgaataaac
     3121 aaaagctgtg gggtgcctgc gttcagcttt atacgctgcg atcggaaaaa aactggggta
     3181 ttggggattt tggcgatctc aaagcgatgc tggtggatgt ggcaaaacgt ggcgggtcgt
     3241 tcatcggcct gaacccgatt catgcgctct atccggcaaa tccggagagt gccagcccat
     3301 acagcccgtc ttctcgccgt tggctgaatg tgatttatat cgacgttaaa gccgtcgaag
     3361 atttccatct tagcgaagag gctcaggcct ggtggcagtt gccgaccacg caacagacgc
     3421 tgcaacaagc gcgggatgcc gactgggtcg attactccac ggttaccgcc ctaaaaatga
     3481 cagcattacg aatggcgtgg aaaggtttcg cgcaacgtga tgatgagcag atggccgcgt
     3541 ttcgccagtt tgttgcagat caaggcgaca gcctgttctg gcaggcagcc tttgatgcgc
     3601 tacatgccca gcaagtgaaa gaggacgaaa tgcgctgggg ctggcctgca tggccagaga
     3661 tgtatcagaa cgtggattca ccagaagtgc gtcagttctg cgaagaacat cgtaatgacg
     3721 tcgattttta tctctggttg cagtggctgg cttacagcca gtttgccgcc tgctgggaga
     3781 taagccaggg ctatgaaatg ccgattggct tgtatcgtga tctggcggtt ggcgtagcgg
     3841 aaggtggggc ggaaacctgg tgtgaccgtg aactatattg cctgaaagcg tcggttggcg
     3901 cgccgccgga tatcctcggc ccgttggggc agaactgggg attaccgcca atggacccgc
     3961 atatcatcac cgcgcgtgcc tatgaaccgt ttatcgagct gttgcgtgcc aatatgcaaa
     4021 actgcggcgc attacgaatt gaccatgtga tgtcgatgct gcgtttgtgg tggataccgt
     4081 atggcgagac ggcagatcag ggcgcgtatg ttcactatcc ggtggatgat ctgctctcga
     4141 ttctggcact cgaaagtaaa cgtcatcgct gtatggtgat tggtgaagat ctcggtaccg
     4201 taccggtaga gattgtcggt aagctgcgca gcagcggtgt gtactcctac aaagtgctct
     4261 atttcgaaaa cgaccacgag aagacgttcc gtgcaccgaa agcgtatccg gagcagtcga
     4321 tggcggttgc ggcgacacat gacctgccta cgctacgcgg ttactgggag agcggggatc
     4381 taacgctggg caaaaccctg gggctgtatc cggatgaagt ggtactgcgc ggtctgtatc
     4441 aggatcgcga actggcgaag caagggctgc tggatgcact gcataaatat ggttgtctgc
     4501 caaaacgtgc cgggcataag gcatcgttga tgtcgatgac gccgacgctg aaccgtggtt
     4561 tgcagcgcta cattgccgac agtaacagtg ctctgttagg actacagccg gaagactggc
     4621 tggatatggc cgaaccggtg aatattcctg gcaccagtta ccagtataaa aactggcgac
     4681 gcaagctttc cgcaacgctt gagtcgatgt ttgccgatga tggcgtgaac aagttgctga
     4741 aggatttgga cagacggcgc agagctgcag cgaagaagaa gtagagtgcg gttgatgccg
     4801 gatgcgatgc atccggcagt cagtgttatc aaatcaccat attcagcagc agacagccta
     4861 ccagaccgca caccgagata atggtttcca gcatcgacca ggatttgatg gtctcgccga
     4921 tagtcaggtt aaagtactct ttgaacagcc agaagcccgg atcgttcaca tgagagaaaa
     4981 tcacactacc ggaacccacc gcaataacca tcagctctgg gctaacaccc gtcgttgcaa
     5041 tcagcggtgc cgcgatacca cccgcagtga ttgccgcaac ggttgcggaa cccagcgcga
     5101 tacgcagtac ggcagcaatc gaccaggcca tcagcagcgg agaaatgttg gtttcgtgca
     5161 tcatggaagc aatgtatttg tccacgccgc tgtctaccag cacctgcttg aacgcaccgc
     5221 caccaccgat gatcaacagc atcatcgcaa tgattttgat ggaagaaacc agcgtgtcgt
     5281 taatctgatc cattgaacga ccacggttca gaccaaaggt gaacatcgca atcagcacgg
     5341 caatcagcgt tgccattacc gggtcaccga ggaactccgc taccggcagg aaagcgtgac
     5401 ctttcggcag gatcatttcg gcaatcgcac gcatcgccat cagcacaacc ggcaccagag
     5461 aagtccagac gctgacgcca aagctcggca tctcttcttc gctgaaggtt ttcgcgctgt
     5521 agagaccttc cgggattggc ttatcgatac ctttcaacac gcgagcgtaa accggaccgg
     5581 cgagaatcac ggtcgggatt gccagaatag taccgtacag cagggtttta cccatatcgg
     5641 cattgaaaat ggtggcaatc gcagtcgggc ccggatgcgg tggcaggaag ccgtgggtca
     5701 cagacagtgc agccgccatt ggtacaccaa catacagcag tggaatattc gcggaagccg
     5761 cgatggtaaa caccagcggc agcatcagca cgaagcccac ttcatagaac agggcaaaac
     5821 caacggtaaa accggttaac accaccgccc actggatgtg ctttttacca aatttggcaa
     5881 tcagcgtggt ggcgatacgt tgtgcgccac cgcagtctgc cagcattttg cccagcattg
     5941 cgccaaaacc catgatcagg gcaaggctac cgagcgtccc gccgacaccg actttgatgg
     6001 agtcaataac tttatccagc ggcattcctt gcattaatcc aacagcaagc gccacgagga
     6061 cgagagcgat gaagccgttc attttgaagc ggatcatcag gagcaacaac aagattacac
     6121 cgatagcaac aatgactaat ggcatgattt acctggcctt tcatttgtta tgggtaacgt
     6181 caattttctg acgacaaact ctaattatcc caatcgggaa cagagatatt gcggcaccac
     6241 gactgatacc cactaaaact aattattgta gtcagatgtc aggagtatgt ttggtaccca
     6301 tgtgaatgat acgggtaaca tttggcgttt gagaatcacc agagcggggt aaatttaaat
     6361 tatgagaggt tggtcatatt atcgcgggga aacgaaggga gggatttgcc aaagcaatgc
     6421 tgtgccaacg tctggcacat gttcaacgta ggcccgaaat gacgctttag cgtcgcatcg
     6481 ggcaatctac aaaagagggg ataacttagt agtaggagtg ttcgccgcgc tggtgttcgg
     6541 tgagatcgcg cacacctttc agctccggga attcgttcag cagctgcttc tcgatccctt
     6601 ctttcagcgt cacatcgacc atggaacaac cgttacagcc gccgccaaat tgcagaatgg
     6661 cgtaaccgtc ttcggtgatt tccatcagcg aaacgcgacc accgtgacca gcaagctgtg
     6721 ggttgatctg cgactgcagc atatactcca cgcgctccat cagcggtgca tcgtctgcca
     6781 ctttacgcat tttggcgttc ggggctttca gcgttaactg agaacccaac tggtcggtaa
     6841 caaagtcgat ctctgcatct tccaggtatg gtgcgcttaa ctcatcaaca tacgcggtca
     6901 gcaggtcaaa tttcagggca gtgtcggtgg cttccacggc atccggcgga caataagaaa
     6961 caccacattc agcgttaggc gtgccagggt taatcacaaa tacgcggatt tgtgtccctt
     7021 cttcctgatt tgccagcagt ttggcaaagt gcgcttgtgc agcatcggaa atacggatca
     7081 tagtaatggc ctaatagttg actattttag ttggttataa tacgcccatc atcgaggctc
     7141 tacaaggttc gacaaaggca ccagacctgg acagtcgccg caccattgcg taaaagcaac
     7201 tgcgcaatct ctgcgacggt acttccggtg gtaacgacat catccacaat caccatatgg
     7261 cgaccttgca cgggcaattc aagacgaaag gcatttttca ggttgcgctt gcgcagccgg
     7321 gcactgagaa aatgctgggt cgcagtggcc cgtgtacgtg tgacggcttc gctatcccat
     7381 tggcagtgca accagcgtga taacggctga cacagcaaat cgctctgatt aaatccccga
     7441 cgccagtgac gccgctgcca taacggaacg ctgatgatgc gatccggcaa ttgcaacccg
     7501 gtggtgcgac gagcgtgtaa gacttccaat agtaacagac gtgacagggc gctggcgatt
     7561 tcactgcgcc gggaaaattt aagctggtgg atgagcagac ttaacggcgg cgcatagtcg
     7621 gcaaccgtga ccagtctttg ccagggcggc ggtttttgca ggcagcgacc gcagggaaga
     7681 tgggagtgtg tggcgggtaa tccacattgt gggcataacg ttttatctgt gcgggtggcg
     7741 cgtgaacaga ccgaacaaat cccccaatga cctaacgcca gtggcattcg gcatagccag
     7801 cataatcccg gtactgttag catatgttca tccttgtaag tcaaaagaga acaatagcgg
     7861 atgaataaca tttggtggca gaccaaaggt caggggaatg ttcatcttgt gctgctgcac
     7921 ggatggggac tgaatgccga agtgtggcgt tgcattgacg aggaacttag ctcgcatttt
     7981 acgctgcacc ttgttgatct gcccggcttc gggcgcagcc agggatttgg tgcgctgtca
     8041 cttgctgata tggccgaagc cgtgctgcaa caggcacctg ataaagccat ttggttaggc
     8101 tggagtctgg gcgggctggt ggcaagccag attgcgttaa cccatcccga gcgtgttcag
     8161 gcgctggtca ccgtggcgtc gtcaccttgt tttagtgctc gtgacgattg gccggggata
     8221 aaaccggacg tgctggcggg atttcagcag caactcagtg atgattttca gcgtacagtg
     8281 gagcggttcc tggcgttaca aaccatgggg actgaaacgg cgcgccagga tgcgcgggcg
     8341 ttgaagaaaa ccgttctggc gttaccgatg ccggaggttg acgtgcttaa tggcgggctg
     8401 gaaatcctga aaacggttga tctccgtcag ccgctgcaaa acgtgcccat gccgtttttg
     8461 cgattgtatg gctatctcga tggtctggtg ccgcgcaaag tggtgccgat gctggataaa
     8521 ctctggcctc gcagcaaatc atatatcttc gccaaagcgg cccatgcgcc gtttatttcg
     8581 catccggtcg agttttgtca cctgctggtg gcgttgaagc agagggtgta ggcaactttt
     8641 gaaatggcga gacagaaatt tactggctcg ccgcagccaa ctcttcttct gacaccccgg
     8701 taaagcgcat gatgtccgca agagggactc cggattcaag cattattttg gctatatgca
     8761 gggctttgga ttgttccccc tcctgccgca atctttccgc aattgtcatt aaactctcct
     8821 tgtgtttcgg tgaacgttcg gcaacgccgt cgataaaatc gttaaaacgt accgcgtcgc
     8881 cggtttgcag tatgtaatta aacagccctt tgatttgtct gtcattagcg tatccactac
     8941 ttaataagca ggccatttgc tctaccagcc ccatcaggt
//
LOCUS       DAZRUF010000166         8962 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZRUF010000166 DAZRUF010000000
VERSION     DAZRUF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.166"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..350
                     /locus_tag="ACHXOL_003109"
     CDS             <1..350
                     /locus_tag="ACHXOL_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR6810029.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
     gene            complement(359..1666)
                     /gene="ynjE"
                     /locus_tag="ACHXOL_003110"
     CDS             complement(359..1666)
                     /gene="ynjE"
                     /locus_tag="ACHXOL_003110"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR6810030.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(1733..2386)
                     /locus_tag="ACHXOL_003111"
     CDS             complement(1733..2386)
                     /locus_tag="ACHXOL_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR6810031.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(2386..3922)
                     /locus_tag="ACHXOL_003112"
                     /pseudo
     CDS             complement(2386..3922)
                     /locus_tag="ACHXOL_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            complement(3895..5061)
                     /locus_tag="ACHXOL_003113"
     CDS             complement(3895..5061)
                     /locus_tag="ACHXOL_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR6810032.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(5071..5619)
                     /locus_tag="ACHXOL_003114"
     CDS             complement(5071..5619)
                     /locus_tag="ACHXOL_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR6810033.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(5619..6326)
                     /locus_tag="ACHXOL_003115"
     CDS             complement(5619..6326)
                     /locus_tag="ACHXOL_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR6810034.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(6341..7018)
                     /gene="ydjY"
                     /locus_tag="ACHXOL_003116"
     CDS             complement(6341..7018)
                     /gene="ydjY"
                     /locus_tag="ACHXOL_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR6810035.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(7023..7733)
                     /locus_tag="ACHXOL_003117"
     CDS             complement(7023..7733)
                     /locus_tag="ACHXOL_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR6810036.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(7900..8706)
                     /gene="xthA"
                     /locus_tag="ACHXOL_003118"
     CDS             complement(7900..8706)
                     /gene="xthA"
                     /locus_tag="ACHXOL_003118"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR6810037.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         2174 a         2394 c         2335 g         2059 t
ORIGIN      
        1 tcttttacgc gctggtgccg tttggcttta ttcttgctgc accggagcaa aatgcgctgg
       61 caggcggctg gttgttgttt gcgtttatcg gcacgggcag cagttttctc gcctttgctg
      121 cactggcagc aaaacatcag attgataacc ccggttatgc acataagtcg ttttactatc
      181 tggggggatt aaccgaaggc acagaaacga tcttactgtt tgtgctggga tgtttattcc
      241 ctgcatggtt tgcgtggttt gcgtggatct tcggtgcgtt gtgctggatg acgaccttta
      301 cgcgggtgtg gagtgggtat ctgacgctga agtcgctcca gcgtcagtaa ttgcaatgtt
      361 atttgctact gtccgggccg cgttcaccgg ttgctaccgg attttttgga tcgctgctcc
      421 attcgtacca gccgccgtca tacacggaaa cattcttcca acccatggcg cgtgcgtaca
      481 taaaggtttc ggacgcgcgc cagccggtgc cgcagtagaa tgaaacttgc tgctctggtt
      541 tgatattcca cgctttccac atagcggtaa tatcatcggc gctgcgcatg gtgccatccg
      601 ggttatgaaa atcttccata tgcgtcgagt cgctaccagc gtgtccccaa cgtgctccgg
      661 ctatttcacc ttttggttta atatagctgt aaccgctggt cgtaccgata aattctggcc
      721 acgaacgaat gctcaccagc gatgcatcct ggcgatgcag cagtccacgg gcctgttcca
      781 tatcgagcat caactgcggt tgtgccggga tcttcacgcc gaaatccggt tccgctttca
      841 ctttcggtgg cgttcctcgc tcaacaggca gtcccgcgtc ggaccaggtt tgccagccgc
      901 catccagcag acgcacatct ttcacgcctg cgtagagcat aatttgcgcc acacgcgctc
      961 cagcgtatac gtcacgcccg tacagaatga ccgtggtgtc atggcgaatg ccgtgttttg
     1021 ccagcatcgc ttttagttgt tcatcagaaa ctttgttcca cagcggttcg ctttccactt
     1081 cgttggtatc gatgtagtca gcaccgggaa tatggctgat aaggtaaagc ttaggagcgc
     1141 cccaggccgc ttcaatgact ttccagtcac cggcaggttt cgccgtaacc tctttacctt
     1201 gttgcaggtc gtgcagccat tgcggataaa ccagctgctc aaagtgcggc agtttttgta
     1261 gacgggaagg ttcgcttagc gcgtcactca gaatggagat atgcgtaaaa cctgcttttt
     1321 gcagtcgcgt tttgacggcg tcgacatctt tgtcattacc gtacagcgcc accggggcat
     1381 cggctttcag gttatgttgc ttgatccacg cgttgagctg ttcggtgctc attttgtcga
     1441 gccagctggc agagaggttt aaggcggcag gttcatgacc agaagggcca tttaaggttt
     1501 gtggccagcc gttataaaac gcgctggggc gagtatctat cgctttgcca ttttgttgtt
     1561 gaagctggtc aagtgtaaga ggcttcgcca gttcagcggc ccacgaagaa gcgcaagcca
     1621 gccctaaagc cattgccagc gcggtcattt gagaaacacg tttcatcgaa aatcccgaca
     1681 ttgtagtttt gagtgcgcca ttctgaactg acgcattgag aaaaactttg cgttatcgca
     1741 gtttgttgta attttctgac cactgcgcca tatccagaac agaactatca gcaggaacat
     1801 cctggagatc gtgcgttacc tgaacgacgg ggatcgccag ttcgcgaact tcgctgaaca
     1861 cccactggcg aaaattatcg cgcagagcca catcaagacg gctgaatggc tcatccagga
     1921 gtaacgcttt tggctgagcg agaagggcgc gcagcagggc cacccgcgct cgctgaccgc
     1981 ctgataatgt cgcagggtcc tggtgataag tttcagccag tcccgctcga tcaagcgcgt
     2041 ctttcacggc attgcgcctg gcagtcccct tgagcgtcga cagtagcgcc agcagtaaat
     2101 tttgcccgac gctgaactgg tcaaataaca gtgcatcctg gaaaagaatg ccaatctgac
     2161 gctgtgcggt gggtagcatg tcaatccgtt gctcattgag ccatagctca cctgtacaag
     2221 aaaactgtcc agccagtgca ccaatcatcc atgaaaacag agtggatttt ccacagccag
     2281 acggccccat taacgtgacg atgtcacctt tatcaaccgt aaagttaacg tttgtcagca
     2341 agcggctttc tggtaaacgt agcgaaacat ttttcacgca gagcattagc ggagtccttg
     2401 tctgacataa cctacccatt ttgcgactaa cgcggtcagg gcaaaaataa taagcggtaa
     2461 caacagttgc cataaagcct gggcggcgag aataccgttg ctgccgccgc tgcttaatgc
     2521 caccgcctca gtggtgagcg tcggaaaacg ccccgcgccc agccacagcg tcggcatata
     2581 ctgcgcaata ctgactgaaa atcccaccgc gaaggcaatc agcacagggc gcaacatgag
     2641 tgggcatttc acgtaaaaga agattttggc ccgcgaccag cccagtgttt gcgcaatcaa
     2701 aattaaccgt gaatcaatgc gctgccaggc gggttgcagg ataaacagca tccacggcat
     2761 tacccacagc agatgccccc agaccaccgc cgtccagctt ccatctaggt tcagccatag
     2821 cgccagcgtg tactggcccg ccaccagtgg cagatcgggc aatagaatgg gcaaccatag
     2881 ccacaactgg cgacgctgtg gcccccattc cagccacagc agtaacagga gcaaagcgat
     2941 gaatgttgct accagcccca ttgtcagact gttgatgagt gcttcactat tgatcgtcga
     3001 ctgatccgcg aggatcgcca gcagaaccac acacagcaca ccggttaagg gtaaaaaact
     3061 cgccagcgta gtgcctggta ataaaggtgt ggcaggcatt gcgaatgcca tctacgcggg
     3121 gaatagtgcg ccgccagctg cgccatagca gatagctcag caaaacgtag gcggcgagta
     3181 acagcatcag cagcagactg gcgagtgcgc ctttcgtttg ttgatccgcg tcgccctgag
     3241 ttaaccactg ccagctaatt actgccagcg tcggtggatt acctggccca agaataatcg
     3301 ccacatccac gaccgaaagc gaccaggcaa caatcgccag catcgccatt gccagcgaag
     3361 gcgcgacgga gggcaacagc agccagttca ggcattgcca gcggctgtag cccagcgaat
     3421 ccagcacaat gacctgctgc aacagccgtt tttcgctcaa tactgccgct aagatccaca
     3481 gcagaaatgc gctttctttc actgcaaggg tgaggcccag accgatgcca aatcggtcca
     3541 ttggcggagt gaaatacggg aaatagtcat atagcagccc tccgtcagca aagagcagca
     3601 gggcgctggt ggcaaaagcc acatggggaa tggcgagcag ccacggcaga cgggcgcaca
     3661 tacgctgcca tttcggccca ggccatagcg ccacaatcac cagcagggca atcaacaatg
     3721 ccccgaccgc cgcgatggtt gtcgatacca gcgttgccag taatgcatgc ggtaactgtg
     3781 gatcggcaaa taacgcctgc cagtgtgtca acgacaacgc aggcgatatc agcgtgagag
     3841 ctgccgggat cagcggtgca taaatcaccg ccaccatcgc ccacagcaaa aagattaatg
     3901 cgtaccgtaa cggcgtaacc attcttgttc cagtgcattc acccatcctg cgtgcggttc
     3961 agccagtacc ggcggcagat cctgcggcat tcttgattgc aatatttcgc gctgcccgtc
     4021 aggcagtttt tgcggatcga gaacagaagg atcgccccag acaacgggat ctgctttacg
     4081 cagttgcgca tcgggtgaga gcaggaaatt ggcaactacc ttcgccgcag cactggcatt
     4141 cgcgttggct ggaatggtga cgaaatgcac gttgccaatc atcccctcgc gaaagccaaa
     4201 actgtaactg cttgccggca aatcgccgct ggcgattttt tgctgcgcat gcgcggggtt
     4261 aaaggtcagc gacaggcgca atgtgccggc tttcagcaga gcatccatcc gcgcgggcga
     4321 aggcgggaaa tcctttcctt cgcgccacaa atacggatgc agtgcatcaa gatattgcca
     4381 caagggcgca gtgacacggg cgaaagtcgc atcgtccggc gcttctttta atgcggcggg
     4441 atctggcgtc agcataatca gcaactgttc aagaaacgcc gtgccggtaa agtccggtgg
     4501 gcgcggataa gtaaccgtgc cgggattggc tttagcaaac tccagtaagg cttgcggcgt
     4561 ttgtggtggc tgtggcgtaa catcgcggcg ggcgataaac gtcagttgtg cgccgcccca
     4621 gggcgattcc gcaccttgtg tcggcactga aaaatcttcc cgcaccggca gctgtgtgtc
     4681 gacatagcgc cagttgggca gagtctccgc ccagcccgtt tgtaataaat tggcctcttt
     4741 taaggtgcgg aagttttcgc cgttcaccca gagcagatcc accgagccgc ccgttttacg
     4801 accggcagca gcttcggtct gaatgcgctt caccgcgtct gcggcatccg cgagacgaac
     4861 aatcttcagg tttatagcgt aatgggtttt catctcgccg ctaacccagt cgagatagcg
     4921 gttaattgcg gtatcgccgc cccaggcgtt aaaccagacg gtttgccctt tggcctcatt
     4981 tttaatttcc tgccagtcac tggcatgtgt tgccaaagaa aacagcgata acaatcccag
     5041 caaccaccca caatggcgca tcttctctcc ttaagaacgt ttttccggaa tacggcacag
     5101 cccctgagcg ggaatgtcca tggcagaatt aaaacgagcc gacaggtttt gcaggccgat
     5161 caatgccgtc agttcggtga gcgcctgagc gtcaaaatgc gcagccagtc gggtacgcag
     5221 ggcatcatcg actgttggcg gtgtgacgct tgcggcttcg gcgtactcca gcgccagccg
     5281 ttcttcatcg ctaaagagcg ggctttgccg ccagtcagcc actgccagca gtttatcgct
     5341 gctgccggta cgctcggcga ctttcatact ggtgatatcc acacaaaact cacacaggca
     5401 catttgcgca atgcgcgcgc tgacaagcga tcgcactacc ggatcgagcg gtgagcgttt
     5461 gcgctccagc cacccaacaa acattgacac caaataaaag ataaacggga tccgccccca
     5521 ccagcgaatc gggcttagca cctcgccgta atggcgacga tgcagccacg cctgcggacg
     5581 taaaatgaaa ggaattttgc taagcggcgg taaacccatc agtcattcct cttctggcgc
     5641 tcaagccata ttttcttcgc cataaaaatc accacggtca gggcaaacag aataaacagt
     5701 ccggtgacaa accagaaagt accgcctgtt aacatgctgc ccgcccagga ataaacaata
     5761 gtcgtcggta attgaccaag cccggtggcg ataaaaaacg agcgaaaacg tattgaagtc
     5821 agatcggcag catagctgat tggatcgaaa gggacaaaag gcaataaccg acagaccagg
     5881 atggtgtgtt tgccgtagcg agtgaaaaag ccgtccatac tgtcaagcac ggttttgccg
     5941 gttaattttt ccaccacttc gcggcccatc actctggcga taaaaaagca cagcgccgcg
     6001 ccggccatcg aactggtcca cgacagcaag ccgccccaga acgcgccaaa cagcgacgca
     6061 ttggcaaagg tgatcaaaaa cgcaggtagc ggtgcagcaa tggcctgtaa aatcatcaac
     6121 aagaacgaga caactgccgc cagtgcgccg taagactgaa taaagcgttc tataccctgt
     6181 tggtccacgg cggcaaacgc cgcaacgctg cgattgataa actcatggac gccgggaagc
     6241 agcgcccatg cgagcagcat agcgaacaac aggatgatga gggttatacg gtagtaccag
     6301 attttacgcg attgcatctt catcatcctt tatcctggct ttattcggtg attttaaagg
     6361 taaccgttgc cagcgtgtta tccgccggga gaactgaggc attgcctttg aatttcactt
     6421 cgccgcgttt ttccaccgca ccataagtgt atgttgcatt gctgacgatg ccgaccgggc
     6481 agctatccag acacaccagg caacccgttt tcttctcttc tgctgccgtt aaattaccgc
     6541 caaagcgcat gtccagtttc ttgccattac tgtcaacaat cacttcatcg aaggaatacg
     6601 cttttgccgc cccttgccag ttgaccgaaa tatccagttt gctgcctgtg acatgagtcg
     6661 tttctttatt atccatcgtc atgttttcgc ccggcgtacc acctgcctct ttcagggctt
     6721 cataaaatgc tttcggggtc gcataagcca taaacagcga tttatgtccg ttggagccat
     6781 ctttaaacac aataccgtga cgagtgtcgt cggtgaggaa acgaccatta atttgcacca
     6841 acatggttac cgtatggtta ttggcatcga cgcgcagcgg ttggctgttc gaaagtccgt
     6901 cgtattccac ttttgccgcc gcattctctt tttgatcgca gccgctaagc ccggcaacag
     6961 ccagtaagca gagtgcagcc agtccttttt tccatgacac tgaataatgt tgcgacatcg
     7021 ttctatcctt cattttttgg gtgagtaagc gggctgcggc gcgaagcaga cagatccaca
     7081 tgtttgtgtc gggcgtagag ttttgcgagc tggacgagaa taaacagcgc caggcccgcc
     7141 agacagagtt gtaaaataaa gcgcagcgta atgccttcat tggcgagatc gcttgccatc
     7201 acggtataaa taacaatacc gggcagggtc gtgagtgccg aaataagggt ataaggccag
     7261 aaggcgatgg tggttaatcc gtaagcgtaa ttttgaatat tgtaaggaaa caacgggatt
     7321 aagcgggtca gaataagaaa atcaataccg ttacgcgcaa tgcctttttc aatggcctgg
     7381 aaggtatggc tatggcccac gtatttcagc agtaaatcac gtcccagcca ccgcgccagc
     7441 aggaatgagc acgaggaggc cagcgtggcg gcaattaatg agagtagtgt ccctaagagc
     7501 gggccaaaaa ctattcctcc agcgatcacc agtatgcttc ctggtaacac aaagagggtg
     7561 gcaatgatga ataacagaat atagaggcta taaccgaaca atccgctctg gcgaatgagt
     7621 gtctgtaagt ggggtaaatc ggtgagcaga tcgaataaac cgaaagcgtg gatagcgtaa
     7681 atgaccagcg caaaaataag acaggcgaaa agaaatttac gctcagcatt catcgtggag
     7741 tttcctatat caaacttgat aatagagaag tggtcagaca actaaaaacg cagtgacggt
     7801 ataacaaagg acggcaggca acaaatcatt actgacagtt tgttgatttt tctcaaggtt
     7861 aattctcctg acccagtttg agccaggaga gctgctaaat tagcggcgga aggtcgccca
     7921 gacgggggcg tgatcggacg gtttttccat gctgcgaatt tcatagtcga tgccggtttc
     7981 tacgcaacat tctgccagcg gttggctggc gagcagcagg tcgatgcgca gaccacggtt
     8041 atcgtcaaaa ccttttgagc ggtaatcaaa ccatgagaaa cgatctgctg tttgcggatt
     8101 agcatggcgg aaggtatcga ccaaccccca gctcatcagc ctgtccatcc attcgcgctc
     8161 ttccggcagg aaagagcatt taccggtacg cagccagcgc ttacggtttt cttcgccaat
     8221 gccgatatcc agatctgtag ggctgatatt catatcgccc ataatcagta ccggattatc
     8281 acgtttgagt tcggtttcca ggtagttttg cagattctga taaaactgcg cttttgccgg
     8341 gaattttatc ggatggtcgc ggctttcacc ctgcgggaag taaccgttga tcacggtgac
     8401 attacccagc agtgagggga tttccgccat aataatccgc cgctgcgcct cttcgtcgtc
     8461 accgggaaag ccgcgacgca cggcaatcgg cgtctctttg gtcagcagcg ccacgccata
     8521 atggcctttc tgcccgtgat aaaacacgtt gtagccgagc ttcgccacct cttcgagcgg
     8581 aaacatatcg tcatgaactt ttgtctcctg caggccaatc acatccggtt ggtgcttttc
     8641 gacgatggct tcaagctggt gaggtctggc gcgcaggccg ttgatattaa aagagacaaa
     8701 tttcatagtc gctgccattt attcagataa agagtgcgtg gatggtagca gaatttcgcg
     8761 ttgcttaccg cctgttgtta agtgatttga tcgatggttg ttaatgatgt caacgatggc
     8821 gcaaaaaatg cccgcttttg gtgcgcgctg cgtcagaatg gcgcagtaat ttccagtaaa
     8881 tttcgtcaat gatcacaatt ccagaattat attttacttc tgcataacat tgcgtttttt
     8941 attcttttta ttcacccaca tc
//
LOCUS       DAZRUF010000167         8783 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZRUF010000167 DAZRUF010000000
VERSION     DAZRUF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.167"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            71..1469
                     /gene="ulaA"
                     /locus_tag="ACHXOL_003119"
                     /pseudo
     CDS             71..1469
                     /gene="ulaA"
                     /locus_tag="ACHXOL_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            1485..1790
                     /gene="ulaB"
                     /locus_tag="ACHXOL_003120"
     CDS             1485..1790
                     /gene="ulaB"
                     /locus_tag="ACHXOL_003120"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR6810038.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1800..2261
                     /gene="ulaC"
                     /locus_tag="ACHXOL_003121"
     CDS             1800..2261
                     /gene="ulaC"
                     /locus_tag="ACHXOL_003121"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR6810039.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVADVVEPRYYQ
                     AILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDILI
                     TMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2275..2920
                     /gene="ulaD"
                     /locus_tag="ACHXOL_003122"
                     /pseudo
     CDS             2275..2920
                     /gene="ulaD"
                     /locus_tag="ACHXOL_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
     gene            2930..3784
                     /gene="ulaE"
                     /locus_tag="ACHXOL_003123"
     CDS             2930..3784
                     /gene="ulaE"
                     /locus_tag="ACHXOL_003123"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR6810040.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            3784..4470
                     /gene="ulaF"
                     /locus_tag="ACHXOL_003124"
     CDS             3784..4470
                     /gene="ulaF"
                     /locus_tag="ACHXOL_003124"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR6810041.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            4567..5118
                     /locus_tag="ACHXOL_003125"
     CDS             4567..5118
                     /locus_tag="ACHXOL_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR6810042.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(5193..5468)
                     /gene="yjfY"
                     /locus_tag="ACHXOL_003126"
     CDS             complement(5193..5468)
                     /gene="yjfY"
                     /locus_tag="ACHXOL_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR6810043.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5795..6190
                     /gene="rpsF"
                     /locus_tag="ACHXOL_003127"
     CDS             5795..6190
                     /gene="rpsF"
                     /locus_tag="ACHXOL_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR6810044.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            6197..6511
                     /gene="priB"
                     /locus_tag="ACHXOL_003128"
     CDS             6197..6511
                     /gene="priB"
                     /locus_tag="ACHXOL_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR6810045.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            6516..6743
                     /gene="rpsR"
                     /locus_tag="ACHXOL_003129"
     CDS             6516..6743
                     /gene="rpsR"
                     /locus_tag="ACHXOL_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR6810046.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6785..7234
                     /gene="rplI"
                     /locus_tag="ACHXOL_003130"
     CDS             6785..7234
                     /gene="rplI"
                     /locus_tag="ACHXOL_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR6810047.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(7346..8317)
                     /locus_tag="ACHXOL_003131"
     CDS             complement(7346..8317)
                     /locus_tag="ACHXOL_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR6810048.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(8370..>8783)
                     /locus_tag="ACHXOL_003132"
     CDS             complement(8370..>8783)
                     /locus_tag="ACHXOL_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR6810049.1"
                     /translation="WAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         2118 a         2153 c         2337 g         2175 t
ORIGIN      
        1 aaaattagag tgagtgcaca acattccggg tgtgcggaat acccggttac ctcttcttca
       61 ggagatcgtt atggagatcc tctacaacat ctttaccgtg ttttttaacc aggtcatgac
      121 caatgccccg ttgttgctgg gtattgtgac ctgtctgggc tacatcctac tgcgcaaaag
      181 tgtcagcgtt attattaaag gcacgattaa aaccataatt ggtttcatgt tgttgcaggc
      241 agggtccggc atcctcacca gcaccttcaa accggtggtg gcgaaaatgt ccgaagtcta
      301 cggcattaaa cggcgcaatt tccgatactt acgcgtcgat gatggcaacc atcgaccgca
      361 tgggcgatgc ctatagctgg gtaggttacg cggtgttgtt agcgctggcg ctgaacatct
      421 gttacgtgct gctgcgtcgc attaccggta ttcgtaccat catgctgact ggtcatatca
      481 tgttccagca ggcggggctg attgccgtta cgttgtttat tttcggctac tccatgtgga
      541 caaccattat ctgcacggcg attctggttt cgctctactg gggcattacc tccaacatga
      601 tgtacaagcc gactcaggaa gtgacggacg gctgcggttt ctccatcggt caccagcaac
      661 agtttgcatc atggactgcc tataaagttg cgccgttcct cggcaaaaaa gaggaaagcg
      721 ttgaagacct caaactgcca ggctggctga atattttcca cgacaacatc gtctccacgg
      781 cgattgtcat gaccatcttc tttggtgcca ttctgctctc cttcggtatc gacaccgtgc
      841 aggcgatggc aggcaaagtg cactggacgg tctatatcct gcaaaccggt ttttccttcg
      901 cggtggcgat cttcatcatc actcagggtg tgcgcatgtt tgtggcggaa ctctctgaag
      961 cgtttaacgg tatctcccag cgcctgatcc ctggtgcggt tctggcgatt gactgtgcgg
     1021 ctatttatag cttcgcgccg aacgccgtgg tctggggctt tatgtggggc accatcggtc
     1081 agctgattgc ggttggcatc ctggtcgcct gcggttcctc gatcctgatt atcccaggct
     1141 ttatcccgat gttcttctcc aacgccacca tcggcgtgtt cgctaaccac ttcggcggct
     1201 ggcgtgcggc gctgaagatt tgtctggtga tggggatgat cgaaatcttt ggttgcgtct
     1261 gggcggtgaa acttaccggt atgagtgcct ggatggggat ggcggactgg tcgattctgg
     1321 caccgccgat gatgcagggc ttcttctcca tcggtatcgc ctttatggcc gtcatcattg
     1381 taattgcact ggcttatatg ttcttcgctg gccgcgcact gcgcgcagaa gaagatgcag
     1441 aaaaacaact ggcagaacag tctgcttaat aaggagtttt gattatgacc gtacgtattc
     1501 tggctgtgtg tggcaacgga caaggcagtt ccatgatcat gaggatgaaa gtggaccagt
     1561 ttttaaccca atcaaacatt gaccacacgg taaatagctg cgcggttggc gaatacaaaa
     1621 gcgagttgag cggcgcggat atcatcatcg cttctacgca cattgcgggc gaaatcaccg
     1681 tgaccggcaa caaatacgtg gttggcgtgc gcaacatgct ctctcctgcc gactttggcc
     1741 cgaaactgct ggaagtgatc aaagcgcatt tcccgcagga tgtgaagtaa ggacgcgcca
     1801 tgaaattacg tgattcgctg gcggagaata aatccatccg cctgcaggct gaagctgaga
     1861 catggcagga cgcagtaaaa atcggcgttg acctgctggt ggcggatgtt gtcgagccgc
     1921 gttactacca ggcgattctg gatgcggttg aacagcacgg tccgtacttt gtgctcgcac
     1981 caggcctggc aatgccgcac ggtcgcccgg aagagggcgt taagaaaacc ggttttgcgc
     2041 tggtgacgct gaaaaagccg ctggagttca accacgaaga taacgacccg gtggatatcc
     2101 tcatcaccat ggcggcggtc gatgccaaca cgcaccagga agtgggcatc atgcagatcg
     2161 tcaacctgtt tgaagatgaa gaaaatttcg accgtttacg tgcctgccgt accgagcagg
     2221 aagtactgga tctcattgac cgcaccaacg cggcagctta agaaggaatt gaagatgtca
     2281 ttaccgatgt tgcaagtcgc gctggacaac cagactatgg atagcgccta cgaaaccact
     2341 cgcctgattg ccgaagaagt cgacattatc gaagtgggta ccattctgtg cgtgggcgaa
     2401 ggcgtgcgtg cggttcgcga tctgaaagcg ctctacccgc acaaaatcgt gctggcagac
     2461 gccaaaattg ccgatgcggg caaaattctt tctcgtatgt gcttcgaagc caacgctgac
     2521 tgggtgaccg tgatttgttg tgcggatatc aacaccgcca aaggtgcgct ggacgtagca
     2581 aaagagttta acggcgacgt gcagatcgaa ctgaccggtt actggacctg ggaacaggcg
     2641 caacagtggc gcgacgcagg tattcagcag gtggtttatc accgcagccg tgacgcgcag
     2701 gccgctggcg tggggtgaag ccgatatcac tgcgatcaaa cgtctttccg atatgggctt
     2761 caaagtcacc gtcaccggtg gcctggcgct ggaagatctg ccgctgttca aaggcattcc
     2821 aattcatgtg tttatcgccg gtcgcagcat ccgtgatgcc gcttctccgg ttgaagccgc
     2881 gcgtcagttc aaacgttcca tcgccgaact gtggggctaa ggagcggata tgttgtccaa
     2941 acaaatcccg cttggcatct atgaaaaagc gctccccgcc ggggagtgct ggctggaacg
     3001 cctgcgactg gcaaaaacgt tgggcttcga ttttgtcgag atgtcggtag atgaaactga
     3061 cgagcgcctg tcgcgcctcg actggagccg cgagcagcgt ctggcgctgg tgaatgcgat
     3121 tgttgaaacc ggcgtgcgtg tgccgtccat gtgcctttct gcccatcgtc gtttcccgtt
     3181 gggcagcgaa gatgacgcgg tgcgggcgca ggggctggag attatgcgta aagccatcca
     3241 gttcgcccag gacgttggca ttcgcgtgat ccagctggcg ggctatgacg tttactatca
     3301 ggaagccaat aacgaaacgc gtcgtcgttt ccgtgacggc ctgaaagaga gcgttgagat
     3361 ggcgagccgc gcgcaggtga cgctggcgat ggagatcatg gattatccgt tgatgaactc
     3421 catcagcaag gcgctgggtt acgcgcacta tctcaacaac ccgtggttcc agctctaccc
     3481 ggatatcggc aacctgtcgg cgtgggacaa cgatgtgcag atggagttgc aggccggaat
     3541 cgggcatatc gtcgcggtgc atgtgaaaga caccaaaccg ggcgtcttca aaaacgtgcc
     3601 atttggcgaa ggtgtggtgg atttcgaacg ttgcttcgaa acgctcaaac agagtggcta
     3661 ttgcgggccg tacctgattg agatgtggag cgaaacggcg gaagacccgg cggcagaagt
     3721 ggtgaaagcg cgtgattggg tgaaagcgcg catggccaaa gcaggcacgg tggaggcggc
     3781 ataatgcaaa agctaaaaca gcaggtgttt gaagccaaca tggatctgcc gcgctatggg
     3841 ctggtgacct ttacctgggg caacgtcagc gctatcgacc gcgaacgcgg gctggtggtg
     3901 atcaagccca gcggcgttgc ctacgaaacc atgaaagcag acgatatggt ggtggtcgat
     3961 atgagcggca aggtggtgga aggggagtat cgcccgtctt ccgacaccgc gacgcatctc
     4021 gaactctacc gtcgttaccc gtcgcttggc ggcattgtcc atacccactc cactcatgct
     4081 actgcatggg cgcaggcggg gctggcgatc ccggcgttag gcactacgca cgccgactac
     4141 ttctttggcg acatcccgtg tacgcgcgga ttaagcgaag aagaggtgca gggcgagtat
     4201 gaactgaaca ccggcaaagt gattatcgaa acgctgggtg acgccgagcc gctgcatacg
     4261 ccggggattg tggtgtatca gcacggtccg ttcgcctggg ggaaagatgc ccacgacgcg
     4321 gtgcataacg cggtggtgat ggaagaagtg gcgaaaatgg cgtggattgc gcgcagtatt
     4381 aacccacaac tgaatcacat cgacagcttc ctcatgaata aacacttcat gcgtaagcac
     4441 ggtcctaacg cctattacgg gcagaagtaa gcagtgcgcc ccggaattat tacctcctgc
     4501 gggatgtaat tggtctgatt ccggggccgc ttcagttaaa aaatcgtata aacaacaggt
     4561 gtttttatgg ctatcaatgg attgtttgtc agaacggatc cgcaacgtcg ccgctatggc
     4621 gttgcgttac ttgtgggtgt actttcagga attatttcag catttgttaa atggggagcg
     4681 gaagtcccct tgccaccgcg cacattttcc ggtggtcgcg atgaatttaa cccgccgtat
     4741 atctttctac gggattattt aggtatcgac ccaacccaaa ctgtgtatac attctcggaa
     4801 catattatca acccggtgat ggtgacgcat atcatcttct cactggtttt tgcgataggg
     4861 tattgtattg tcgcggaaat tttcccgaaa gtgaaattat ggcaggggat attagcaggg
     4921 cttattgtga cagtggcagt ccacggtata ttttgcccgg cgctgaattt aacgccgccg
     4981 ctcactcaat tgccttttga cgaatatgcc tctgaaatcc tggggcatat tttctggttc
     5041 tggataatag aaattatgcg ccgcgactta cgtaatcgca ttactcatga gccagatccg
     5101 gaagtcgcaa tccggtgaat aaccgcataa aaaagccccc tgcgaagggg gcaaagcaaa
     5161 ctatggcaat gtttcgttgg ttatacctgg tgctagcgat aaatatccgc gctggcgtgc
     5221 atattgccgt tactccccgg ttcccgcatc agaattacgt ggtagtacgt tgcgccctgc
     5281 gcatctgtct cttcatttaa tgcctgacgc gcttcgcttt cagtggcgaa attatgattg
     5341 atataaatca cgcctaagct ttgcacatca tccatgtttc tggcctggtg gttattaatt
     5401 tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc taaaactcga
     5461 ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctctgtg atgatcttct
     5521 gatttaattt taatcaatga taaagaagtt gatggtgacc atttctgatg cagttgttca
     5581 aaaaaacacc atgatgaagt gtgatgaact ccaaatcagc ggtttagagg ttaattgcga
     5641 aaggggagat ttatttcggc tctgcccttg agtttagcgg ggcatacaag tactataacg
     5701 cgtcattttt cagccgacct ttaacacgtt ccttgcctcc ccgggattcg gctgacccag
     5761 acaggaggct gaataatccg taaggagcaa ttcgatgcgt cattacgaaa tcgtttttat
     5821 ggtccatcct gaccagagcg aacaggttcc gggcatgatc gagcgctaca ctgctgccat
     5881 cactggtgca gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc agctggctta
     5941 cccgatcaac aaactgcaca aagcacacta cgttctgatg aacgttgaag ctccgcagga
     6001 agtgatcgat gagctggaaa ctaccttccg cttcaacgat gccgttatcc gcagcatggt
     6061 tatgcgtacc aagcacgctg ttaccgaagc atctccgatg gttaaagcga aagacgagcg
     6121 ccgtgagcgt cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag ctggggattc
     6181 tgaagagtaa tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt gcaggactcc
     6241 ccttcgaaag gtcagtccat caggaattcc tcactgccag ttcgtgcttg agcatcgttc
     6301 tgtgcaggag gaagccggtt ttcaccggca ggcgtggtgc caaatgcccg ttattgttag
     6361 cggacacgaa aaccaggcca ttactcacag tataacggtc ggcagtcgca taaccgttca
     6421 ggggttcatt tcatgccaca aggcaaagaa cggactgagc aaaatggttt tgcatgccga
     6481 gcagattgaa ttgatagatt ctggagacta gccatatggc acgttatttc cgtcgtcgca
     6541 agttctgccg tttcaccgcg gaaggcgttc aagagatcga ctataaagat atcgctacgc
     6601 tgaaaaacta catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc ggtacccgtg
     6661 caaaatacca gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg tccctgctgc
     6721 cgtacactga tcgccatcag taatcggtca cggtccatta atacgacttt gagaggataa
     6781 ggtaatgcaa gttattctgc ttgataaagt agcaaacctg ggtagcctgg gtgatcaggt
     6841 aaacgttaaa gcgggctatg ctcgtaactt cctggtaccg cagggtaaag ctgttccagc
     6901 taccaagaaa aacattgaat tcttcgaagc acgtcgcgct gaactggaag ctaaactggc
     6961 tgaagttctg gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg aaactgttac
     7021 catcgcgtct aaagctggcg acgaaggtaa actgttcggt tccatcggta ctcgcgacat
     7081 cgctgacgct gtaactgcag ctggcgttga agtggctaag agcgaagttc gtctgccgaa
     7141 cggcgttctg cgtaccactg gcgagcacga agtgagcttc caggttcaca gcgaagtatt
     7201 cgcgaaagtg atcgtaaacg tagtagctga ataattcgtt attcaacgag acgtaaaaac
     7261 gccgaccagt ggtcggcgtt ttgcttttgt agcgagcact tgcagatatc gataagggcc
     7321 acaataagca gccctacgaa gataattact tagctgcctg aatatcagcc agcattgggg
     7381 caagggctgg gttgtcctta gcgaagtttg cgtacattgg ctgtactttc tcgataaacg
     7441 gagctttatc tacctggtag actttcacgc ccattttatc cagttttgca aattcttgtt
     7501 tctcttcctc gctccacaag tctttcatgt agctcattga ttctttagct gcttttaata
     7561 taactgcctg attttccggg ctaagtttat caaacgcagc attgctcatc agaaccacat
     7621 cgggaaccat agtgtgttcg tcaaggctga aggattttgc cacttcaccg tgacgcatgg
     7681 tggtcagcgc catcacgctg ttttctgcca tatcgaccac accctgctgt aaggctgtat
     7741 acaactcgcc gtaatccagt ggcgtcgggt taccgcccat gactttgatc atttcaactg
     7801 cgccagggct tggctggaca cggactttca tgcctttcag atcgtctggt ttcagtactg
     7861 gtttattgcc atagaaactg cgggcgcctc catcataaaa agtcagcccg aaataacctt
     7921 tgcttttcgt tgagtcgagg atttttctcc cgatatcgcc ctgcatgacc tgataataat
     7981 gatcacgatc gcggaacaaa taaggcaggc taaataattt ataggaattt tcaaatgctt
     8041 ccatttctgc cgcattggtt tttaccagtg gaatagcgcc agaacgaatc agctccattg
     8101 tttctcgttg agtacccaat gttccatttg gataaatacg aatagtaata tcaccgttag
     8161 agtactcttt gttctttttc gcaaagaact ccatcgcttt atgaacagga tgagacttat
     8221 cctgattatg actcagtttt aatgtcactg cccaggactg gcttgcggat aacataagaa
     8281 cagcaccgat aatgccagcg gatactttta ttttcataga tgttcctttt ctaataaata
     8341 tggcggcagg tgccgccagt gaatgcgcat cagtagccca gtgcttttgg caatgccata
     8401 ctgatttgcg gaatataggt caccattgcc agcaccaata ccatcacggc atagaatggc
     8461 agcataggtt tgataatttt attaatcggt aatttagata cactacaacc gacaaataac
     8521 acactgccta caggcggagt acaaataccg atacacatgt tcagtaccat gacaatacca
     8581 aagtgaatcg ggtccatgcc cagttcggtg acaacaggta aaaatatcgg cgtaaaaatc
     8641 agtaccgccg gtgtcatatc cataaaagtg ccgataatca acaggataat attgataata
     8701 agcaggatta ttattgggtt gtcagaaata gtgttaagca aatcgagaat caggaaggga
     8761 acgtcagcgt ttgacattgc cca
//
LOCUS       DAZRUF010000168         8762 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZRUF010000168 DAZRUF010000000
VERSION     DAZRUF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.168"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHXOL_003133"
     CDS             complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHXOL_003133"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR6810050.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATF"
     gene            1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHXOL_003134"
     CDS             1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHXOL_003134"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR6810051.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(4998..5648)
                     /gene="rcsB"
                     /locus_tag="ACHXOL_003135"
     CDS             complement(4998..5648)
                     /gene="rcsB"
                     /locus_tag="ACHXOL_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR6810052.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(5665..8337)
                     /gene="rcsD"
                     /locus_tag="ACHXOL_003136"
     CDS             complement(5665..8337)
                     /gene="rcsD"
                     /locus_tag="ACHXOL_003136"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR6810053.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAKQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2145 a         2041 c         2247 g         2329 t
ORIGIN      
        1 gaaggttgcg ccgtagcccg gcaaactggc gacgcgaata tcaccctgat gggcattaat
       61 gatgcgttga cttaacgcca gaccaagccc ggttcctgag gctttggtgg tgaaaaaggg
      121 atcgaagatc tttttttgca gcgagagatc aatgccacag ccgttgtcct ctatcgaaat
      181 ggcctgttgt gagtcgctgt attgccaggt ttgaatgcga attttccctc gtgcgctgat
      241 agcctggacg gcattgatca ggatattcag tagtacctgt ttgagcagtt cacgatcggc
      301 gttaatcggg cttaattcat tatccagttc gcttatgaag tcgacccgcg cttgtacgcc
      361 ggcggtttgt accagtacca gagtttcttc aaccaatgca ttgaggctga cttgttgcca
      421 ttgactgtgg cgtggacgtg aaaattcgag caattgctga ataactttgt taattgaatc
      481 gatttctttg agtactacgg acagatattc ctgatgtatt gggtcactgg tttgttggcg
      541 caagatctgt acataaccac gaatagccgt taacggatta cgtacttcat gcgcgacgcc
      601 agccatcagc tcacccagtg tggcgaggcg ttctgcttgc gccatgcggc gctgggtttc
      661 tttgcgggca gttaaatcag agaaaatcac caaagcacct atcatttcac cgtgcgtgtt
      721 atgaatacga ctggtagtga cactgagttc aatggtgcgg tcacgacctg gaaaactgat
      781 ctccagcgcc acatgttcgg tgccatgttc cagcgtatcc agtactggac tgtagaactg
      841 agtattgtcg aacaacatgg agtaaggctg ccctaccagt tcatggcgtt gatagccagt
      901 gataacttct gctgctgggt tcatggtggt tacatcaccc tggcggtcaa tggcaatgac
      961 gccatcggca gcgttttcaa taatcagatc gttaagtgtc cgcgtttcac gcagtgcctg
     1021 ggcgaggtta ttaacactct gactgatttg ccccatttca ccgggcaatt gtggtaatcg
     1081 agtgggaata ttttgtgcca gagtcgagag gccatcggtg atgatatcaa tattggcgct
     1141 caggcgacgg gagaaaagga caatcaatag caggcttatc agcaaatcgg cggtgagcac
     1201 aatgataatc ctcacatcca ttttccaggc ctggcggcga atatcttcgg ttaattcatt
     1261 ggcccagata tagccgagga tttcaccatt acgctcaatg gggagcattg aattcaaaat
     1321 atcgccgcgc acctgcctgc ctgaataaac caaaggggta tttgtacgca tgacttcgcg
     1381 accagggtga tctgcggcaa tggtaacgcc gacattattc tgatatagcg ctgaaggcgc
     1441 gtaggtgatt atcgcatcca gcattttgtt gtaataacca gcacctatgc cagggaaggc
     1501 gtgagtgata ttttcggtaa tgggggcaag ttctgcattt aatgcgcgga tacgctcctc
     1561 acgtggtaag tcgatgtaga gatcatagcg atcgcctagt gcctgattaa gcaggttgac
     1621 cacggcagaa agttttttct ctttttcaga taagactgct gaacgtcctt ccgtttctac
     1681 gatataacca atagtaagcg ttgggacaat gaccatcagg attgccatca ggatcatttg
     1741 attgcgtaag cggcgtggat aaatccactt catataatgc atgctgaaat ccactagtct
     1801 tgtccggtat atgacgatta tcagaggtta aggtgatgat ttctcggcgg tgtatcatat
     1861 tccagagaag agagaacatt gcggtaacac gcttttaccg ctaccttaac cacactccat
     1921 cggtcacctg aggcggagct tcgccccttt gaaatacctt gcttcttttc gtacaaccct
     1981 gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg ctctggctgt tgattgcttt
     2041 ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga
     2101 atttaatctg agttccgatc aggctcagcg ctttattcaa cgcacctctg atgtgatgaa
     2161 agagctgaag tacatcgccg aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg
     2221 tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg ctgtttgccg actccgattg
     2281 ttccgcaatg agtaacacct ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta
     2341 ctggcgcgat aatttttctg cggcttacga tctaaaccgg gtatttttaa tcggcagcga
     2401 taacctctgc atggccaatt tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt
     2461 gaaagctttg catgaacgca tcaataaata tcgaaatgca ccacaagatg atagcggcag
     2521 taacctctac tggatcagcg aaggtccgcg ccctggcgtc gggtattttt acgcgttgac
     2581 gccagtttat ctggcgaacc ggttgcaggc gcttttgggt gtcgagcaga ccatccggat
     2641 ggagaacttt ttcttaccgg gtacgttgcc gatgggggtt accattcttg atgaaaatgg
     2701 tcataccctg atttcgctta ccggaccaga aagtaaaatt aagggcgatc ctcgctggat
     2761 gcaggaacgc tcctggtttg gctatacgga agggttccgg gagctggtgc tgaagaaaaa
     2821 tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg gttgataagg tgctggaacg
     2881 cattcgcatg ttgatcctta acgcaatttt gctgaatgtg cttgccggag ctgcattgtt
     2941 tactctcgca cggatgtacg agcgacgtat tttcattccg gcggaaagcg acgccctgcg
     3001 actggaagaa catgagcagt tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg
     3061 cattttgcgt accgctgatg gcgtcaatat tttaagtaac gaactggcgc atacctatct
     3121 caatatgctt acgcatgagg accgccaacg actgacgcaa attatctgtg ggcagcaggt
     3181 caattttgtt gatgtcctga ccagcaacaa taccaatctg caaatcagct tcgtccattc
     3241 gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa
     3301 gatggaagag tcgttgcagg agatggcaca agcagcggaa caggcgagcc agtcaaaatc
     3361 gatgttcctt gccaccgtca gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa
     3421 cctggatctg ttgcaaacca aagagttacc gaaaggcgtc gatcggctgg tgacggcaat
     3481 gaacaactct tccagcctgt tgttgaaaat tatcagcgat attctcgatt tctcgaagat
     3541 tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca
     3601 catcaccgcc aactatttac cgctggtggt acgcaagcag ttaggcttgt actgctttat
     3661 tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg cgtttacagc aggtcatctc
     3721 caacctgttg agtaacgcca taaaattcac cgataccggc tgtatagttt tgcatgtccg
     3781 cgctgatggc gattatctct ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa
     3841 agaagtggtg cgcttgtttg atcccttctt ccaggtcgga acgggcgtac agcgaaactt
     3901 ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg atcagcatga tggacggcga
     3961 tatctcggta gattcagaac cgggaatggg cagccagttt accgtgcgta ttccgttgta
     4021 cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg agtggtaaac gctgctggct
     4081 ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc agtttgcagc gcagcggcat
     4141 cgtcgttaca acatacgaag ggcaggaacc gactcccgaa gatgtgttaa ttactgacga
     4201 ggtagtgagt aaaaaatggc agggtagagc ggtagtgacc ttctgtcgtc gccatattgg
     4261 tattccgctg gagaaagcgc caggggagtg ggtacacagt gtggctgccc cgcatgagct
     4321 accggcattg ttggcgcgta tttatttgat cgagatggag agcgacgatc ctgctaacgc
     4381 tctgccgtcg acggacaaag cggtcagcga taatgacgat atgatgattc tggtcgtgga
     4441 tgatcatccg attaaccgac gtctgctggc agatcagttg ggatcgttgg gctatcaatg
     4501 taaaaccgcg aatgatggtg tcgatgcgct taatgtactt agcaagaatc atattgatat
     4561 cgtgcttagc gacgtcaaca tgccaaatat ggatggttac cgcttgacgc aacgcattcg
     4621 tcagttggga ctgacgttgc cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa
     4681 gcagcggtgt ctggagtccg gtatggacag ctgcctgtcg aagccggtaa cgctggatgt
     4741 gataaaacag acgctgacgg tatatgccga gagggtcagg aaatcgcggg aatcgtagga
     4801 cggataaggt gtttatgccg catctggcat tcagtgcaaa tgccagatgc gacgctgacg
     4861 cgtcttatct ggcctacttt gatgtaatct gttgaaataa tgggaatcgt aggacgcata
     4921 aggcgtttac gccgcattcg gcaatcggtg caaatgccag ataagacact aacgcgtctt
     4981 atctggccta caggtgatta gtctttatct gccggactta acgtcactga agagagataa
     5041 ttcagcaggg cgatatcgtt ctcgacaccc agcttcatca tcgcagattt cttctggcta
     5101 ctgatggttt taatactgcg gttcagcttt ttagcgatct cggtcaccag gaagccttcc
     5161 gcaaacaggc gcagaacttc actctctttt ggcgagagac gcttgtcacc gtaaccacca
     5221 gcactgattt tttccaacag gcgagaaacg ctttccgggg taaatttctt ccctttctgc
     5281 agcgcggcga gagctttcgg cagatcggtc ggtgcacctt gtttcagcac gatcccttcg
     5341 atatccagat ccaataccgc actaagaatc gccgggttgt tgttcatagt cagaacaatg
     5401 atcgacaggc ttgggaaatg gcgcttgatg tacttgatta aggtaatgcc atcgccgtac
     5461 ttatcgccag gcatggagag atcggtaatc aacacatgcg catccagttt cggcaggttg
     5521 ttgatcagtg ctgtagagtc ttcaaattcg ccgacaacat tcacccactc aatttgctca
     5581 agtgatttgc gaataccgaa caagactatc ggatggtcat cggcaataat tacgttcata
     5641 ttgttcatgt aataggctac cttgctacag caagctcttg acataactgt caatgtcgct
     5701 gatgtatttt tctattcctg gaacatcctt ctcacgaatc agatgttcca gcgtttcaca
     5761 taactgcttg ccgggtacca gatttagcat ggcaaatacg cctttaagac gatgagccgt
     5821 ttgggctaac gcagcaaagt cactggttgc tgcttcagta tacagcctct taacatcatc
     5881 cggtactgtg tctacaaaga gcgcataata gccgctggca tggagttgcg cattttcatc
     5941 tccgcccaga ggcgattctg tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa
     6001 gaccgcttcc tgcatagcgt tgctcatatt gaagttgacg cacaattgac caggcccaat
     6061 ttcccgtacg ccagactcat catcgcttaa aagcaagcca gaggcagtaa gattagacgg
     6121 attatccgtt aaaaagatat cataatcttg actaattaat ctttcatcgg gtgtgataca
     6181 ggttgcaccc caattttcta actggcgagt gacaatattc cgaatttctg ccgaagtaac
     6241 atccaccatt acgcagacat catccagtaa acgctcttct tcctcttcaa cttccgggcc
     6301 agcggcgagc attttgatat gcacagagta gcgtgtacca agcccatccc gcgttttgat
     6361 gtttaaatga ccgcccagtt tacgtgccag ttgatcgctc agccagaatg ccagcgggtc
     6421 cgcctttcca taacgatcgt tttgggtctg gttgataaac gggaagtgca aattatccat
     6481 ttcatgaata cttacgcctt ctccggtgtc cagaatgcgg aatgtcaggc ggtcttcaga
     6541 ggactcatcc tgatcaacct caagggtgat ttttcccaat tgcgttgagg tcacggcata
     6601 ttgcatcagt agcagcaaaa tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg
     6661 tgctttcaga tgattgttaa tcagcagttg cagaccttta cgcttgatgg caggcaacac
     6721 tgaaggcaca acttcatcaa ttaaatcctg cacggagaac agcaccgtct cacttttcca
     6781 gctatcgtcc gcaagcatgt tcgctaactg tatttcatcg accagccgca ccagcacatc
     6841 tgcctgattc gccagttgtt tgctttccgg ggcgttgagt ttagccgcgc tctccgccag
     6901 ggactgtgca ggttctttca gcgcatcgcc aatgtttttc ataaaggtca tccggccctg
     6961 ctggtttttc tcatacagac gctgcgcctg cttgagtttc ttgtttacca gcacttcgcg
     7021 atcctgatcg cgaataatga aaatttgtgt gcgcggcgcg acctggctgc ggaacatgcg
     7081 gatctcatac agctcgttat tgatcgtcgc ctgaataatc ccctgatgct gtttcgccat
     7141 ggtggtgatg ttttgcagat tcaaatgcgg cagcaaatga tcggcaattt tgttacttat
     7201 gacagtgcgg ttcgattcct gatcgtgaac cagcaggccg agcggcagca gtgagactat
     7261 ctcttcattg attgcccgta aaatgcgcaa ttcgttattg accgccgtgt tgggtagact
     7321 ttctgtactg cgactggaga aatggcggaa tgtggtatag ccaaataacg ccagcgccag
     7381 caaaccgatg ttcagcagca gtggcagcag aatgttttgc aacgtatcca gcaataaggt
     7441 gccataagga acctgccaga ccaggcgcat atcggtagag ttgagtgccg aggagatttc
     7501 aatcttcgta ctgttaaagt ggatactgac gctatccgtt ccttctttct cattatcatt
     7561 gtctcccgtt gccgtcgtgt ctggctcaag gcggaaactg tccagcggca tacccggtgg
     7621 gatcaaatca ttaatcggca gatcaaaagc cacgaccgtt gccagatgtc ccggctggtt
     7681 gaacgtggta cgcaaggtaa agtaatgacc gttctgccag gccaggcggc gcatgttaga
     7741 aaaactttcg cgttcatcga gggcgttggc ctgttgcaac atttctgcac gacgtgaatc
     7801 aacaatgtca ctgacggtcg attctttaaa tccggaggtg agatctttga ggggcagggt
     7861 tgagatcagt accagactgt tatcctgacc attcaggtaa tacatcgacc acggtacatt
     7921 ttctgcaccc cacaatgtat ccagataagt ggacatccgc tgagtcatct caagcgttga
     7981 gttgtcgtga gagccaaaga ttaacgcttc cgttttgcgg cgtggtttct ccagatagta
     8041 gacatcctgt ttcaggcgcg tctcttgtaa accttcgccg gaggaggggg aggtcgtcgc
     8101 ggcaatgttg tcgtagatct gccaggtcac gtaacgccag gtatcgacgc gcttttggat
     8161 tgcgtgggta atgtcgacaa tctggtaact tttatctttc agccaggcgt taacggcgct
     8221 ttgtaccatt acccccatcg tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt
     8281 aatgctcccc ggtaggagtg aaaagcgggt cgtggccgtt gtctctttct gacgcattcg
     8341 tgtttatgac ctgttaaaac ttcgcgaagt gatagggcag tataaagggt acagtgtgaa
     8401 attccagact cttacctgat tgatgattct ctattctccc gtgatattta tcatcctgca
     8461 ttgggggaaa tagatgtaca agcgccattt tgctgtacaa ataacctaca aaaagcccaa
     8521 cgaaattaat tgcaaataaa cgaagattgc tggaaattat gctgatgtta attatttgtg
     8581 aaatagttga caagagttat agtttttctg tggtagcaca gaataatgaa agtgtgtaaa
     8641 gaagggtaaa aaaaaccgga tgcgaggcat ccggttgaaa taggggtaaa cagacattca
     8701 gaaatgaatg acggtaataa ataaagttaa tgatgatagc gggagatatt ctagttgcga
     8761 gt
//
LOCUS       DAZRUF010000169         8697 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZRUF010000169 DAZRUF010000000
VERSION     DAZRUF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.169"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..135
                     /gene="yhaV"
                     /locus_tag="ACHXOL_003137"
     CDS             <1..135
                     /gene="yhaV"
                     /locus_tag="ACHXOL_003137"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR6810054.1"
                     /translation="MNDENTLRTYGKKTNAYTVFSKMLKRGHPPADWETLTRETEETH
                     "
     gene            complement(190..999)
                     /gene="agaR"
                     /locus_tag="ACHXOL_003138"
     CDS             complement(190..999)
                     /gene="agaR"
                     /locus_tag="ACHXOL_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR6810055.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            1248..2528
                     /gene="kbaZ"
                     /locus_tag="ACHXOL_003139"
     CDS             1248..2528
                     /gene="kbaZ"
                     /locus_tag="ACHXOL_003139"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR6810056.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCNMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGDAHETLSELAVTTPDAARATLEAHRHAFKKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            2551..3024
                     /gene="agaV"
                     /locus_tag="ACHXOL_003140"
     CDS             2551..3024
                     /gene="agaV"
                     /locus_tag="ACHXOL_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR6810057.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            3035..3814
                     /gene="agaW"
                     /locus_tag="ACHXOL_003141"
     CDS             3035..3814
                     /gene="agaW"
                     /locus_tag="ACHXOL_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR6810058.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPSAQKEEFEDGI"
     gene            3804..4682
                     /gene="agaE"
                     /locus_tag="ACHXOL_003142"
     CDS             3804..4682
                     /gene="agaE"
                     /locus_tag="ACHXOL_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR6810059.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            4700..5134
                     /gene="agaF"
                     /locus_tag="ACHXOL_003143"
     CDS             4700..5134
                     /gene="agaF"
                     /locus_tag="ACHXOL_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR6810060.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTCLVDELGRCHEECPVEEGI"
     gene            5131..6264
                     /gene="nagA"
                     /locus_tag="ACHXOL_003144"
     CDS             5131..6264
                     /gene="nagA"
                     /locus_tag="ACHXOL_003144"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR6810061.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCITDGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVFDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAAAWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            6615..7769
                     /locus_tag="ACHXOL_003145"
     CDS             6615..7769
                     /locus_tag="ACHXOL_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFR6810062.1"
                     /translation="MPENYTPAAAATDTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYTPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            7782..8642
                     /gene="kbaY"
                     /locus_tag="ACHXOL_003146"
     CDS             7782..8642
                     /gene="kbaY"
                     /locus_tag="ACHXOL_003146"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFR6810063.1"
                     /translation="MSIISTKYLLQDAQANDYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KCFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2006 a         2325 c         2315 g         2051 t
ORIGIN      
        1 atgaacgatg aaaacactct gcgcacctac ggtaaaaaaa caaatgccta taccgtattc
       61 agcaaaatgt taaaaagagg acatcctcct gccgactggg aaaccctcac ccgagagaca
      121 gaagaaaccc attgatggtg ttcacaatgc ccgccgaacg cattcgtgtt gaccagcggg
      181 caccctttct cactccccga ccagaatcac ttcaacccca gcctttcgca gtccttccag
      241 actatccgca ggaatgcctt catcaacaat gatcatgtcg atacgttgag tatcaatgat
      301 cttatgtaaa ctggagcggt tgaacttact ggaatcggtg accacgatga tccgttccgc
      361 aacttcgcac atccggcggt ttaaacgggc ttcatcttca ttatgtgtgc tgacgccgcg
      421 ctccagatcg atcgcatcta caccaagaaa cagcatatcg aagtggtaat tttgcagcga
      481 ttgctcagcc tgatcgccgt aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat
      541 cagcagctca acgccttcgg cttccagcaa cgcattagcc acgttcatac cgttggtcat
      601 cgcaattacg tcagtgtgct tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga
      661 atcgaggatc acccgatgac ctggctgaat caactcaacg gcagctttcg caacgctgcg
      721 tttcatcgcg gtgttcagtg cgcttttatc ttccactgat ggctcgactg acggcgtcgt
      781 gctatcgcag atcaacgcgc caccataggc acgcacagcg atcccctgct tttccagaaa
      841 cgccagatcg ttgcggatcg tcacagtaga tacgccatac aatgccgaca gatcgttaac
      901 ctgcacactc ccttgctgtc gcaggcgctg aatgatctgt tctcgtcgct cgctggtgcc
      961 tgtcactcgc ttctcacctg aagcgtcggt attactcata gtaagtcctt tcgtaaaact
     1021 ttcgtttcat ttcgttttgc ctattaacgc ctttctatta agcaaatgca agcccacctt
     1081 gcccattgac gcaagctact ctcgtttcag taactttcat tatgtttctt ttgtgaatca
     1141 gatcagaaaa ctattatctt tcgttttatt tttatctcac catggcgcag tatcaactga
     1201 aacaaaacga aagattaata tcgcagtaat ctgaactgga gaggaaagtg aaacatctga
     1261 cagaaatggt gagacagcac aaagcgggca aaacaaatgg aatttatgcc gtttgttccg
     1321 cacatccgct ggtgctggag gctgcaatcc gctacgccag tgcaaaccaa acgccgttac
     1381 tgattgaagc aacctccaat caggtagacc agttcggcgg ttataccgga atgacgcccg
     1441 ccgattttcg cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg caggatgcgt
     1501 tgattctggg tggtgaccat ctggggccaa accgctggca aaacctgcca gccgctcagg
     1561 caatggccaa tgccgatgat ttgattaaaa gctacgttgc ggcaggattc aaaaaaatcc
     1621 accttgattg caacatgtcc tgtcaggacg atccaattcc cttaactgat gacatcgtgg
     1681 ctgaacgcgc cgcccgtctg gcgaaagtgg cggaagaaac ctgtcgcgaa cactttggcg
     1741 aagccgatct ggagtatgtc attggtaccg aagtgccggt acctggcgac gcgcatgaaa
     1801 ccttaagcga gctggcggtc accacgccgg atgccgcccg cgccacgctg gaagcccatc
     1861 gtcacgcctt taaaaagcag ggtctgaatg ccatctggcc acgcatcatt gccctggtgg
     1921 ttcaacccgg cgtcgaattc gatcacacca acgttattga ttatcagccc gccaaagcga
     1981 ccgccttaag ccagatggtc gaaaactacg aaacgctgat attcgaagcg cactctacag
     2041 attatcaaac gccgcaatcg ctgcgccagc tggtgattga tcactttgcc attctgaaag
     2101 ttggcccggc gctgaccttc gccctgcgtg aagccctgtt ttctctggcg gcgattgaag
     2161 aagaactggt gccagcaaaa gcctgttccg gtctgcgtca ggtgctggaa aacgtgatgc
     2221 tcgaccggcc ggaatactgg caaagccact accacggtga cggcaacgcg cgtcgtctgg
     2281 cgcgtggtta tagctactcg gatcgcgtgc gctattactg gccggacagc cagattgatg
     2341 acgctttcgc tcatctggtt cgtaatctgg cggattcacc aattccgcta ccgctcatca
     2401 gccagtatct gccgctgcaa tacgtgaaag ttcgctccgg cgagctgcaa ccgacgccac
     2461 gggaactcat tatcaaccat attcaggaca tcctggcgca gtaccacaca gcctgtgaag
     2521 gccaataagc aaaacaaaga ggaacacgct atgccaaata ttgttttaag ccggattgat
     2581 gaacgcttga ttcacggtca ggtcggcgtt cagtgggtcg gatttgcggg ggcaaatctg
     2641 gtgctggtag ccaacgatga ggttgccgaa gatccggtac aacaaaacct gatggaaatg
     2701 gtactggcag aagggatcgc cgtacgtttc tggacgctgc aaaaagttat cgacaacatt
     2761 catcgcgccg ccgatcgaca gaaaatcctg ctggtttgta aaacacccgc cgatttcctg
     2821 acgctggtga aaggtggcgt tccggtgaat cgcattaacg ttggcaatat gcactacgcc
     2881 aatggcaaac aacaaatcgc caaaacggtt tctgtggatg cgggcgatat cgcagcattt
     2941 aacgacctga aagccgctgg ggtggaatgc ttcgttcagg gcgtcccgac agagcctgct
     3001 gtggacctct ttaaattact ttgagggatt catcatggaa atcagtctgt tgcaggcatt
     3061 cgcgttgggc attatcgcct ttatcgctgg cctggatatg tttaacggcc taacccatat
     3121 gcaccgcccg gtggtcctcg gcccgttggt cgggctggta cttggcgatc tgcataccgg
     3181 aattttaacc ggcggtacgc tggaactggt gtggatgggg ctggccccgc tggcgggcgc
     3241 acagccgcct aacgtgatta tcggtactat cgtcggcacg gcgtttgcca ttactactgg
     3301 cgtgaaaccc gatgtcgcag tcggtgtcgc cgtacctttc gctgtcgcag tacagatggg
     3361 gattaccttc ctgttctcgg tgatgtctgg cgtgatgtct cgctgcgacc ggatggcgga
     3421 gaacgccgat actcgcggca ttgaacgcgt gaactatctg gcactgctgg cactcggcac
     3481 tttctatttt ctctgcgctt tcctgcctat ctacttcggc gcggaacatg caaaaaccat
     3541 cattgatgtc ctgccgcaac gattaatcga cggcctcggc gtcgcgggcg gcatcatgcc
     3601 agcaatcggt tttgccgtgc tgctgaaaat catgatgaaa aacgtctaca tcccctactt
     3661 catcctgggc tttgttgccg cagcctggct caagttaccg gtgctggcta ttgctgccgc
     3721 tgcgctggcg atggcgctga tcgacctgct gcgtaaatct cctgaaccga ctcaaccttc
     3781 ggcacagaaa gaggaatttg aagatggcat ctaatcaaac caccctgccg aacgtcagtg
     3841 aaaacgaaga aacattgctg actggcgtca atgaaaacgt gtatgaagat cagagcattg
     3901 gcgcggagct gacgaaaaaa gatatcaacc gcgtcgcctg gcgttccatg ttgttacaag
     3961 cgtcttttaa ctacgaacgt atgcaggctt ctggctggtt gtacggtctg ctgcctgctc
     4021 tgaaaaagat ccacactaat aaacgcgacc tggcgcgcgc catgaagggg catatgggtt
     4081 tcttcaatac ccatccgttt ctggtgacgt ttgttatcgg cattatcctt gcgatggagc
     4141 gttctaagca ggacgttaac agtattcaga gcaccaaaat tgccgtcggt gcgccgcttg
     4201 gcgggattgg cgatgcaatg ttctggctga cgctactgcc gatttgtggc gggataggcg
     4261 caagcctcgc gctacaaggc tctattcttg gcgcagtcgt ctttattgtg ctgttcaacg
     4321 tggtacacct ggggctgcgt tttggtctgg cgcattatgc ttaccgcatg ggcgtggcgg
     4381 cgattccact cattaaagcg aataccaaaa aagtcggcca tgctgcgtca atcgttggaa
     4441 tgacggtaat cggcgcactg gtggcaacct atgttcgttt aagcaccacg ctggaaatca
     4501 cagcgggcga cgcagtggtt aagttacaga ctgatgttat tgacaaactg atgcctgcct
     4561 tcttaccgct ggtctacacc ctgaccatgt tctggctggt acgccgcggc tggagtccgc
     4621 tgcgcctgat tgcagtgacc gtggttctcg gcatcgtcgg taaattctgc catttccttt
     4681 aaatacaatg aggtattaga tgttaagtat tattttgaca gggcatggcg ggtttgccag
     4741 tggcatggaa aaagcaatga agcaaatcct cggcgatcaa tcgcagttta ttgccatcga
     4801 ttttcccgaa acctcaagca ccgcgctgct tacatcgcag cttgaagagg ccatcgccca
     4861 actggattgt gaagacggca ttgtttttct gacggatttg ctgggcggca cgccgtttcg
     4921 cgtagcttca acgctggcga tgcaaaaacc gggctgtgaa gtgattaccg gcaccaattt
     4981 gcaactgcta ctggagatgg tgctggagcg cgaagggtta agcggtgaag agttccgtgt
     5041 gcaggcgctg gagtgtggac accgtgggct gacctgtctg gtggacgagt taggccgctg
     5101 ccatgaggaa tgtccggtcg aggaaggaat atgacacacg ttctgcgcgc cagaaggctg
     5161 ctgactgaag agggatggct cgatgaccat cagttgtgta ttactgacgg tgtcatcgca
     5221 gcaatcgaac cgattccagt gggcgtgact gaacgcgatg cggaactgct ctgccctgct
     5281 tacatcgaca cccatgtaca cggtggtgcg ggcgttgatg taatggatga cgcgccggat
     5341 gtgttcgaca agctggcaat gcacaaggca cgcgaaggtg tcggcagttg gttaccgacc
     5401 accgtaaccg cgccgcttaa taccattcat gcggcgctga aacgtattgc tcaacgttgc
     5461 caacgcggcg gacctggtgc gcaagtgctg gggagttatc tcgaaggacc gtacttcacg
     5521 ccgcagaata aaggcgcgca tccgccggag ttgtttcgcg agcttgaaat tgccgagctg
     5581 gatcagttga ttgccgtttc tcagcacacc ttacgtgtgg tagcgctggc accggaaaaa
     5641 gaaggggcat tgcaggccat ccgccatctt aaacagcaaa acgtaagggt gatgctgggg
     5701 catagcgcgg cggcctggca acaaacccgc gccgcatttg atgctggtgc cgacggcctg
     5761 gtgcattgct ataacgggat gacaggttta catcaccgcg aaccgggaat ggttggcgcg
     5821 ggattaacgg acaagcgcgc ctggctggaa ctgatagccg atggtcatca tgtgcatccg
     5881 gcggcaatgt cgctgtgttg ttgctgtgcg aaagagagaa tcgtactgat caccgacgcg
     5941 atgcaggccg ctgggatgcc ggatggtcgc tatacgttat gtggtgaaga agtgcagatg
     6001 cacggtggcg ttgtccgtac cgcgtccggt gggctggcgg gcagtacgct atctgtcgat
     6061 gcagcagtgc gcaacatggt cgagttaacg ggcgtaacgc ctgcggaagc catccatatg
     6121 gcgtcgctgc atccggcgcg aatgctgggt gttgatggtg ttctgggatc gcttaaaccg
     6181 ggcaaacgcg ccagcgtcgt tgcgctggat agcgggctgc atgtgcaaca aatctggatt
     6241 cagggtcaat tagcttcgtt ttgatggttt gctccattat tgggccttca cttcccccgt
     6301 aaggcctttc tttttctttc gttttgatct gtgcagcggt gccggatgcg acgctaacgc
     6361 gtctgatccg acctacggta cgccgcatcc ggcaatcggt gaacatagcg acgtcattac
     6421 gcaattcttt aatcttccct tgtcttttct ttcaacgatc acagatttcg ttttatttcc
     6481 cttccctcca ttgaactttc ggtttctttt ctatagattt taatcaacga aagacatcac
     6541 caagtgaaat gaaacgaaag gcaagtgaaa gcgacaacgc ccgacgtcaa gttcatcaga
     6601 ctaaggattg agttatgcca gaaaattaca cccctgctgc cgccgcaacc gatacatgga
     6661 ctgaagaaga gatccgccat cagcctcgcg catggatccg ttcactcacc aacatcgacg
     6721 cgctacgttc cgcgctcaat aacttccttg aaccgttact gcgcaaagag aatctgcgga
     6781 tcatcctgac cggagccgga acgtcggcat ttatcggtga catcatcgcg ccgtggctcg
     6841 ccagccatac cggtaaaaac ttcagcgccg taccgaccac cgatctggtc accaatccga
     6901 tggactacct gaatccagct catccgctgc tgttgatctc cttcggtcga tccggcaaca
     6961 gcccggaaag cgtcgccgcc gtggaactgg caaatcaatt tgtacccgag tgctatcacc
     7021 tgccgatcac ctgcaacgaa gcgggcgctc tttaccaaaa cgcgatcaac agcgataacg
     7081 cgtttgccct gctgatgccc gcagaaacgc acgatcgcgg ctttgcgatg accagcagca
     7141 ttaccaccat gatggccagc tgcctcgcgg ttttcgcacc tgagacgatc aacagccaaa
     7201 ccttccgcga tgtggcggat cgttgccagg cgatcctgac ctcactgggt gatttcagcg
     7261 aaggtgtgtt tggttataca ccgtggaaac ggatcgttta tctcggcagc ggtggcttac
     7321 agggcgcagc acgcgagtcg gcgctgaaag tgctggaact gactgcgggt aaactggcgg
     7381 ccttctatga ttccccaacc ggattccgtc atggcccgaa atcgctggtc gataacgaaa
     7441 cgctggtggt ggtatttgtc tcaagccacc cttacacccg tcagtatgat cttgatctgc
     7501 tggctgaact tcgccgtgac aaccaggcaa tgcgcgtaat cgccatcgcc gcggaaagca
     7561 ccgacgttat cgccgctggc ccgcatatca tcctgccgcc atcccgtcac tttatcgacg
     7621 ttgagcaggc attttgcttc ctgatgtacg cccagacgtt tgcactgatg cagtcgctgc
     7681 acatgggcaa tacgccggat accccatcag ccagtggcac cgttaaccgc gtggtgcaag
     7741 gcgtaatcat tcatccgtgg caggcataag aggatcgcat tatgagcatt atctccacca
     7801 aatatctgtt acaggacgcc caggccaatg actacgcggt gcctgccttt aacattcata
     7861 acgccgagac gatccaggcg atcctcgaag tgtgcagtga aatgcgatcg ccggtgatcc
     7921 tcgccggaac gccggggacc tttaaacata tcgcgctgga agagatctac gccctgtgta
     7981 gcgcctattc cacaacctac aacatgccac tggcgctgca tctcgaccac cacgaatcgc
     8041 tggatgatat tcgccgtaaa gtccacgcag gcgtgcgcag tgcgatgatc gacggcagcc
     8101 acttcccgtt tgccgagaac gtaaagctgg tgaaatcggt tgtcgacttc tgccactccc
     8161 aggattgcag cgtggaagca gaactgggcc gcctgggcgg tgttgaagat gacatgagcg
     8221 ttgacgccga aagtgcattc ctgaccgatc cacaagaagc taaatgcttt gtcgaactga
     8281 ctggcgtcga cagcctggcg gtagcgattg gtacggcgca cggcttatac agcaaaacgc
     8341 cgaagattga tttccagcgg ctggcggaaa ttcgtgaagt ggtggatgtt cctctggtgc
     8401 tgcatggtgc cagcgatgtt ccggatgaat ttgtccgtcg cactattgaa cttggcgtca
     8461 caaaagtgaa cgttgccaca gaattaaaaa tagccttcgc tggcgcggtt aaagcctggt
     8521 ttgcggaaaa tccgcagggt aatgatcctc gttattatat gcgcgtcgga atggatgcga
     8581 tgaaagaagt tgtcagaaat aaaattaatg tctgtggttc agcgaatcga atttcagcat
     8641 aatcattagt tttttatgat ttatcccaat gtacttcccg attaatatcg gggagtg
//
LOCUS       DAZRUF010000170         8654 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZRUF010000170 DAZRUF010000000
VERSION     DAZRUF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.170"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(289..552)
                     /gene="rpsT"
                     /locus_tag="ACHXOL_003147"
     CDS             complement(289..552)
                     /gene="rpsT"
                     /locus_tag="ACHXOL_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR6810064.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            655..873
                     /locus_tag="ACHXOL_003148"
     CDS             655..873
                     /locus_tag="ACHXOL_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR6810065.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            881..1822
                     /gene="ribF"
                     /locus_tag="ACHXOL_003149"
     CDS             881..1822
                     /gene="ribF"
                     /locus_tag="ACHXOL_003149"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR6810066.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1865..4681
                     /gene="ileS"
                     /locus_tag="ACHXOL_003150"
     CDS             1865..4681
                     /gene="ileS"
                     /locus_tag="ACHXOL_003150"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR6810067.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4681..5175
                     /gene="lspA"
                     /locus_tag="ACHXOL_003151"
     CDS             4681..5175
                     /gene="lspA"
                     /locus_tag="ACHXOL_003151"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR6810068.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5300..5749
                     /gene="fkpB"
                     /locus_tag="ACHXOL_003152"
     CDS             5300..5749
                     /gene="fkpB"
                     /locus_tag="ACHXOL_003152"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR6810069.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5751..6701
                     /gene="ispH"
                     /locus_tag="ACHXOL_003153"
                     /gene_synonym="lytB"
     CDS             5751..6701
                     /gene="ispH"
                     /locus_tag="ACHXOL_003153"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR6810070.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6767..7681
                     /gene="rihC"
                     /locus_tag="ACHXOL_003154"
     CDS             6767..7681
                     /gene="rihC"
                     /locus_tag="ACHXOL_003154"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR6810071.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7848..>8654
                     /gene="dapB"
                     /locus_tag="ACHXOL_003155"
     CDS             7848..>8654
                     /gene="dapB"
                     /locus_tag="ACHXOL_003155"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR6810072.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLN"
BASE COUNT         1970 a         2160 c         2464 g         2060 t
ORIGIN      
        1 atagagtggt cttaagttta atacctcaat gtgtatcaca atatccacat tctttgtagg
       61 ggagtctgga gattgagtag atattcttgt tcagaatgta tcagccgatg gttctacgat
      121 tcttaagcca cgaagagttc agaaagtgta acggcatgtc tgcaaaagcc agcatctttt
      181 gactatctgg caaccggtag tgtgttctct cacgcatcat aaaagcagca ggcataaaaa
      241 aaaacccgct tgcgcgggct ttttcacaaa gcttcagcaa attggcgatt aagccagttt
      301 gttgatctgt gcagtcaggt tagccttatg acgtgcagct ttgtttttgt ggatcagacc
      361 tttagcagcc tgacggtcca cgatcggttg catttcgtta aatgctttct gtgcagcagc
      421 tttgtcgcca gcttcgatag ctgcgtatac tttcttgatg aaagtacgca tcatagagcg
      481 acggcttgcg ttgtgcttac gagccttttc agactgaatg gcgcgcttct tagctgattt
      541 gatattagcc aaggtccaac tcccaaatgt gttctatatg gacaattcaa aggccgagga
      601 atatgccctt ttagccttct tttgtcaatg gatttgtgca aataagcgcc gttaatgtgc
      661 cggcactcgt tacgtagtga tggcgcagga ttctaccagc ttgcggggtg tgaatacagc
      721 ttttccgcga taaaaattgc agcaggcggt cactttcttc ccgtgatttg cgccatggca
      781 atgaaaagcc acttctttct gatttcggta ctcaatcgcc ggttaacctt gaccgctgta
      841 caaggtatac tcggacgatt ttcactgttt tgagccagac atgaagctga tacgcggcat
      901 acataatctc agccaggccc cgcaagaagg gtgtgtgctg actattggta atttcgacgg
      961 cgtgcatcgc ggtcatcgcg cgctgttaca gggcttgcag gaagaagggc gcaagcgcaa
     1021 cttaccggtg atggtgatgc tttttgaacc tcaaccactg gaactgtttg ctaccgataa
     1081 agccccggca agactgaccc ggctgcggga aaaactgcgt taccttgcag agtgtggcgt
     1141 tgattacgtg ctgtgcgtgc gtttcgacag gcgtttcgcg gcgttaaccg cgcaaaattt
     1201 catcagcgat cttctggtga agcatttgcg cgtaaaattt cttgccgtag gggatgattt
     1261 ccgctttggc gctggtcgtg aaggcgattt cttgttatta cagaaagctg gcatggaata
     1321 cggcttcgat atcactagta cgcaaacttt ttgcgaaggt ggcgtgcgta ttagcagcac
     1381 cgctgtgcgt caggcgctgg cggatgacaa tctggctctg gcagaaagtt tactggggca
     1441 cccgtttgcc atctccgggc gtgtagtcca cggtgatgaa ttagggcgca ctataggttt
     1501 cccgacggcg aatgtaccgc tacgccgtca ggtttccccg gtgaaagggg tttatgcggt
     1561 agaagtgctg ggcctcggtg aaaagccgtt acccggcgtg gcaaacatcg gaacacgccc
     1621 aacggttgcc ggtattcgcc agcagctgga agtgcatttg ttagatgttg caatggacct
     1681 ttacggtcgc catatacaag tagtgctgcg taaaaaaata cgcaatgagc agcgatttgc
     1741 gtcgctggac gaactgaaag cgcagattgc gcgtgatgaa ttaaccgccc gcgaattttt
     1801 tgggctaaca aaaccggctt aagcctgtta tgtaatcaaa ccgaaacacg gaaccgagaa
     1861 tctgatgagt gactataaat caaccctgaa tttgccggaa acagggttcc cgatgcgtgg
     1921 cgatctcgcc aagcgcgaac cgggaatgct ggcgcgttgg actgatgatg atctgtacgg
     1981 catcatccgt gcggctaaaa aaggcaaaaa aaccttcatt ctgcatgatg gccctcctta
     2041 tgcgaatggc agcattcata ttggtcactc ggttaacaag attctgaaag acattatcgt
     2101 gaagtccaaa gggctttccg gttatgactc gccgtatgtg cctggctggg actgccacgg
     2161 tctgccgatc gagctgaaag tagagcaaga atacggtaag ccgggtgaga aattcaccgc
     2221 cgccgagttc cgcgccaagt gccgcgaata cgcggcgacc caggttgacg gtcaacgcaa
     2281 agactttatc cgtctgggcg tgctgggcga ctggtcgcac ccgtacctga ccatggactt
     2341 caaaactgaa gccaacatca tccgcgcgct gggcaaaatc atcggcaacg gtcacctgca
     2401 caaaggcgcg aagccagttc actggtgcgt tgactgccgt tctgcgctgg cggaagcgga
     2461 agttgagtat tacgacaaaa cttctccgtc catcgacgtt gcttttcagg cggtcgatca
     2521 ggatgcactg aaagcaaaat ttgccgtaag caacgttaac ggcccaatct cgctggtgat
     2581 ctggaccact acgccgtgga ctctgcctgc gaaccgcgca atctctattg cacctgattt
     2641 cgactatgcg ctggtgcaga tcgacggtca ggccgtgatt ctggcgaaag atctggttga
     2701 aagcgtaatg cagcgtatcg gcgtgaccga ttacaccatt ctcggcacgg taaaaggtgc
     2761 ggagcttgag ttgctgcgct ttacccatcc gtttatgggc ttcgacgttc cggcaatcct
     2821 cggcgatcac gttaccctag atgccggtac cggtgccgtt cacaccgcgc ctggccacgg
     2881 cccggacgac tatgtgatcg gtcagaaata cggcttggaa accgctaacc cggttggccc
     2941 ggacggcact tatctgccgg gcacttatcc gacgctggat ggcgtgaacg tcttcaaagc
     3001 gaacgacatc gtcgttgcgc tgctgcagga aaaaggcgcg ctgctgcacg ttgagaaaat
     3061 gcagcacagc tatccgtgct gctggcgtca caaaacgccg atcatcttcc gcgcgacgcc
     3121 gcagtggttc gtcagcatgg atcagaaagg tctgcgtgcg cagtcactga aagagatcaa
     3181 aggcgtgcag tggatcccgg actggggcca ggcgcgtatc gagtcgatgg ttgctaaccg
     3241 tcctgactgg tgtatctccc gtcagcgcac ctggggcgta ccgatgtcac tgttcgtgca
     3301 caaagacacg gaagagctgc atccgcgtac tctcgaacta atggaagaag tggcaaaacg
     3361 cgttgaagtt gacggcatcc aggcgtggtg ggatcttgat gcgaaagaga tcctcggcga
     3421 tgaagctgat cagtacgtga aagtgccgga cacattggat gtatggtttg actccggatc
     3481 tacccactct tctgttgttg acgtgcgtcc ggaatttgcc ggtcacgcag cggacatgta
     3541 tctggaaggt tctgaccagc accgtggttg gttcatgtct tccctaatga tctccaccgc
     3601 gatgaaaggc aaagcgccgt atcgtcaggt actgacccac ggctttaccg tggatggtca
     3661 gggtcgcaag atgtctaaat ccatcggcaa taccgtttcg ccgcaggatg tgatgaacaa
     3721 actgggcgcg gatattctgc gtctgtgggt ggcatcaacc gactacaccg gtgaaatggc
     3781 cgtttctgac gagatcctga aacgtgctgc cgatagctat cgtcgtatcc gtaacaccgc
     3841 gcgcttcctg ctggcaaacc tgaacggttt tgatccagca aaagatatgg tgaaaccgga
     3901 agagatggtg gtactggatc gctgggccgt aggttgtgcg aaagcggcac aggaagacat
     3961 cctcaaggcg tacgaagcat acgatttcca cgaagtggta cagcgtctga tgcgcttctg
     4021 ctccgttgag atgggttcct tctacctcga catcatcaaa gaccgtcagt acaccgccaa
     4081 agcggacagt gtggcgcgtc gtagctgcca gactgcgctg tatcacatcg cagaagcgct
     4141 ggtgcgctgg atggcaccaa tcctctcctt caccgctgat gaagtgtggg gctacctgcc
     4201 gggcgaacgt gaaaaatacg tcttcaccgg tgagtggtac gaaggcctgt ttggcctggc
     4261 agacagtgaa gcgatgaacg atgcgttctg ggacgagctg ttgaaagtgc gtggcgaagt
     4321 gaacaaagtc attgagcaag cgcgtgccga caagaaagtg ggtggctcgc tggaagcggc
     4381 agtaaccttg tatgcagaac cggaactgtc ggcgaaactg accgcgctgg gcgatgaatt
     4441 acgatttgtc ctgttgacct ccggcgctac cgttgcagac tataacgacg cacctgctga
     4501 tgctcagcag agcgaagtac tcaaagggct gaaagtcgcg ttgagtaaag ccgaaggtga
     4561 gaagtgccca cgctgctggc actacaccca ggatgtcggc aaggtggcgg aacacgcaga
     4621 aatctgcggc cgctgtgtca gcaacgtcgc cggtgacggt gaaaaacgta agtttgcctg
     4681 atgagtcaat cgatctgttc aacagggcta cgctggctgt ggctggtggt agtcgtgctg
     4741 attatcgatc tgggcagcaa atacctgatc ctccagaact ttgctctggg ggatacggtc
     4801 ccgctgttcc cgtcgcttaa tctgcattat gcgcgtaact atggcgcggc gtttagtttc
     4861 cttgccgata gcggcggctg gcagcgttgg ttctttgccg gtattgcgat tggtattagc
     4921 gtgatcctgg cagtgatgat gtatcgctcg aaggccacgc agaagctaaa caatatcgct
     4981 tacgcgctga ttattggcgg cgcgctgggc aacctgttcg accgcctgtg gcacggcttc
     5041 gttgtcgata tgatcgactt ctacgtcggc gactggcact tcgccacctt caaccttgcc
     5101 gatactgcca tctgtgtcgg tgcggcactg attgtgctgg aaggtttttt gccttctaga
     5161 gcgaaaaaac aataataaac cctgccggat gcgatgctga cgcatcttat ccggcctaca
     5221 gattgctgcg aaatcgtagg ccggataagg cgtttacgcc gcatccggca aaaatcctca
     5281 aatataagag caaacctgca tgtctgaatc tgtacagagc aatagcgccg tcctggtgca
     5341 cttcacgcta aaactcgacg atggcaccac cgccgagtct acccgcaaca acggtaaacc
     5401 ggcgctgttc cgcctgggtg atgcttctct ttctgaaggg ctggagcaac acctgttggg
     5461 gctgaaagtg ggcgataaaa ccaccttctc gttggagcca gatgcggcgt ttggcgtgcc
     5521 gtcaccggac ctgattcagt acttctcccg ccgtgaattt atggatgcag gcgagccaga
     5581 aattggcgca atcatgcttt ttaccgcaat ggatggcagt gagatgcctg gcgtgatccg
     5641 cgaaattaac ggcgactcca ttaccgttga tttcaaccat ccgctggccg ggcagaccgt
     5701 tcattttgat attgaagtgc tggaaatcga tccggcattg gaggcgtaac atgcagatcc
     5761 tgttggccaa cccgcgtggt ttttgtgccg gggtagaccg cgctatcagc attgttgaaa
     5821 acgcgctggc catttacggc gcaccgatat atgtccgtca cgaagtggta cataaccgct
     5881 atgtggtcga tagcttgcgt gagcgtgggg ctatctttat tgagcagatt agcgaagtac
     5941 cggacggcgc gatcctgatt ttctccgcac acggtgtttc tcaggcggta cgtaacgaag
     6001 caaaaagccg cgatttgacg gtgtttgatg ccacctgtcc gctggtgacc aaagtgcata
     6061 tggaagtcgc ccgcgccagc cgtcgtggcg aagagtcgat tctcatcggc cacgccgggc
     6121 atccggaagt ggaaggtaca atgggccagt acagtaaccc ggaaggggga atgtatctgg
     6181 tcgaatcgcc ggacgatgtg tggaaactga cggtcaaaaa cgaagagaag ctctccttta
     6241 tgacccagac cacgctgtcg gtggatgaca cgtctgatgt gatcgacgcg ctgcgtaaac
     6301 gcttcccgaa aattgtcggt ccgcgcaaag atgacatctg ctacgccacg actaaccgtc
     6361 aggaagcggt acgcgccctg gcagaacagg cggaagttgt gttggtggtc ggttcgaaaa
     6421 actcctccaa ctccaaccgt ctggcggagc tggcccagcg tatgggcaaa cgcgcgtttt
     6481 tgattgacga tgcgaaagat atccaggaag agtgggtgaa agaggttaaa tgcgtcggcg
     6541 tgactgcggg cgcatcggct ccggatattc tggtgcaaaa tgtggtggca cgtttgcagc
     6601 agctgggcgg tggtgaagcc attccgctgg aaggccgtga agaaaacatt gttttcgaag
     6661 tgccgaaaga gctgcgtgtc gatattcgtg aagtcgatta agtcattagc agcctaagtt
     6721 atgcgaaaat gccggtcttg ttaccggcat tttttatgga gaaaacatgc gtttacctat
     6781 cttcctcgat actgaccccg gcattgacga tgccgtcgcc attgccgccg cgatttttgc
     6841 ccccgaactc gacctgcaac tgatgaccac cgtcgcgggt aatgtctcgg ttgagaaaac
     6901 cacccgcaac gccctgcaac tgctgcattt ctggaatgcg gagattccgc tcgcccaggg
     6961 agccgctgtg ccgctggtac gcgcaccgcg tgatgcagca tctgtgcacg gcgaatcggg
     7021 aatggctggc tacgactttg ttgagcacaa ccgaaagcca atcgggatac cagcgtttct
     7081 ggcgattcgt gatgccctga tgcgtgcacc agagcctgtt accctggtgg ccattggccc
     7141 gttaaccaat attgcgctgt tactttcaca atgcccggaa tgtaaaccgt atattcgtcg
     7201 tctggtgatc atgggcggtt cttctggtcg cggcaactgt acaccaaatg ccgagtttaa
     7261 tatcgctgcc gatccagaag cggctgcctg tgtcttccgt agcggaatcg aaatcgtcat
     7321 gtgcggtctg gatgtcacca atcaggcaat attaacccct gactatctcg ctactttgcc
     7381 ggagttgaat cgcaccggaa aaatgcttca tgcactgttt agccactacc gtagcggcag
     7441 tatgcaaagc ggtctgcgaa tgcacgatct ctgcgccatc gcctggctgg tgcgcccgga
     7501 cttattcacc ctcaagccgt gttttgtcgc ggtagaaact caaggcgaat ttacctcagg
     7561 cacgacggtg gttgatatcg acggttgcct gggcaaacca gccaatgtaa aggtggcatt
     7621 ggatctggat gtgaaaggct tccagcagtg ggtggctgag gtgctggctc tggcgtcgta
     7681 acctgtcaca tgttatcggc atgcagtcat tcatcgactc atgcctttca ctgatatccc
     7741 tccctgttta tcattaattt ctaattatca gcgtttttgg ctggcggcgt agcgatgcgc
     7801 tggttactct gaaaacggtc tatgcaaatt aacaaaagag aatagctatg catgatgcaa
     7861 acatccgcgt tgccatcgcg ggagccgggg ggcgtatggg ccgccagttg attcaggcgg
     7921 cgctggcatt agaaggcgtg cagctgggcg cggcgctgga gcgcgaagga tcttctttac
     7981 tgggcagcga cgccggtgag ctggctggtg cagggaaaac aggcgttacc gtgcaaagca
     8041 gcctcgatgc ggtaaaagat gattttgatg tgtttatcga ttttacccgt ccggaaggta
     8101 cgctgaacca tctcgctttt tgtcgccagc atggcaaagg gatggtgatc ggcactacgg
     8161 ggtttgacga agccggtaaa caggcgattc gtgacgccgc cgccgatatt gcgattgtct
     8221 ttgccgccaa ttttagcgtt ggcgttaacg tcatgcttaa gctgctggag aaagcagcca
     8281 aagtgatggg tgactacacc gatatcgaaa ttattgaagc acatcataga cataaagttg
     8341 atgcgccgtc aggcaccgca ctggcaatgg gagaggcgat cgcccacgcc cttgataaag
     8401 atctgaaaga ttgcgcggtc tacagtcgtg aaggccacac cggtgaacgt gtgcctggca
     8461 ccattggttt tgccaccgtg cgtgcaggtg acatcgttgg tgaacatacc gcgatgtttg
     8521 ccgatattgg cgagcgtctg gagatcaccc ataaggcgtc cagccgtatg acatttgcta
     8581 acggcgcggt aagatcggct ttgtggttga gtggtaagga aaatggtctt tttgatatgc
     8641 gagatgtact taat
//
LOCUS       DAZRUF010000171         8634 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZRUF010000171 DAZRUF010000000
VERSION     DAZRUF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.171"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHXOL_003156"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHXOL_003156"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR6810073.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHXOL_003157"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHXOL_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR6810074.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHXOL_003158"
     CDS             4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHXOL_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR6810075.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHXOL_003159"
     CDS             complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHXOL_003159"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR6810076.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHXOL_003160"
     CDS             complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHXOL_003160"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR6810077.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHXOL_003161"
     CDS             complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHXOL_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR6810078.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHXOL_003162"
     CDS             complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHXOL_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR6810079.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2155 a         2247 c         2051 g         2181 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa tagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aagcacttcc ccctttgtgt
     4261 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
     4321 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     4381 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
     4441 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     4501 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
     4561 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
     4621 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     4681 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaaccg
     4741 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
     4801 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     4861 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     4921 ccgcaattta ttatgctgca atacgtcaaa gggaaagcgg aaagttggcc tgggctgtca
     4981 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     5041 gtgttagtag tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     5101 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
     5161 tgcctcggca tcggctatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
     5221 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tattttattt gccgttcttt
     5281 atcctcggtg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
     5341 cgtggctgta cccttgcagc agcattggca tttgtcgctt atttactcaa ccagcgctat
     5401 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
     5461 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     5521 gtgacttact ttgttaacgc atcgctattt atctatctgg ttcaccaccc gttaacgctg
     5581 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
     5641 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccgtta
     5701 ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
     5761 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
     5821 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     5881 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     5941 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     6001 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
     6061 ttttaccatc gatgctaaaa gttttagcat gcaggctggc tccggaatta ccggttatgc
     6121 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
     6181 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     6241 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     6301 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     6361 tgggtgagcc catgatatcg gacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     6421 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     6481 ttccatcaac cagatgattt ataaacggac tggattccat ttggcgtaag taacgatgcg
     6541 tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
     6601 ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
     6661 catcggcaac gtcctctacc acgggtccta ttgccataac atctaaatcc gaaaaaagtg
     6721 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agggtaacca
     6781 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
     6841 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
     6901 ggggatggct gtcaagcaag cgtaaaatat cgtcaagtcc gggcgtattg ttgtcgtcca
     6961 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
     7021 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
     7081 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
     7141 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
     7201 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
     7261 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
     7321 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
     7381 gccactgacg tatagctgtt tgccgccacc agtcgcaggc tattcagata ggcatcctgc
     7441 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgtactacg
     7501 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
     7561 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
     7621 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
     7681 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
     7741 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
     7801 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
     7861 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
     7921 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
     7981 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
     8041 atggatcaca cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaaacaaact
     8101 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
     8161 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
     8221 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
     8281 ccattgttgt gataaatgaa gtgcctgtcc atcagaaaca gtaacaacta ttttcacccg
     8341 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
     8401 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
     8461 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
     8521 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
     8581 cgcattcata ttcttctccc gaaaaaaaca gggcttgcgc cctgtttctg taat
//
LOCUS       DAZRUF010000172         8608 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZRUF010000172 DAZRUF010000000
VERSION     DAZRUF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.172"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(187..1851)
                     /gene="glnS"
                     /locus_tag="ACHXOL_003163"
     CDS             complement(187..1851)
                     /gene="glnS"
                     /locus_tag="ACHXOL_003163"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR6810080.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2110..4056)
                     /gene="nagE"
                     /locus_tag="ACHXOL_003164"
     CDS             complement(2110..4056)
                     /gene="nagE"
                     /locus_tag="ACHXOL_003164"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR6810081.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            4389..5189
                     /gene="nagB"
                     /locus_tag="ACHXOL_003165"
     CDS             4389..5189
                     /gene="nagB"
                     /locus_tag="ACHXOL_003165"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR6810082.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5249..6397
                     /gene="nagA"
                     /locus_tag="ACHXOL_003166"
     CDS             5249..6397
                     /gene="nagA"
                     /locus_tag="ACHXOL_003166"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR6810083.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            6406..7626
                     /gene="nagC"
                     /locus_tag="ACHXOL_003167"
                     /gene_synonym="nagR"
     CDS             6406..7626
                     /gene="nagC"
                     /locus_tag="ACHXOL_003167"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR6810084.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7674..8426
                     /gene="nagD"
                     /locus_tag="ACHXOL_003168"
     CDS             7674..8426
                     /gene="nagD"
                     /locus_tag="ACHXOL_003168"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR6810085.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8477..8608)
                     /locus_tag="ACHXOL_003169"
                     /pseudo
     CDS             complement(8477..8608)
                     /locus_tag="ACHXOL_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2103 a         2271 c         2193 g         2041 t
ORIGIN      
        1 atatttcttg ccggatgcgg cgtgaacgcc ttaaccggcc tacaaaagca cgcaaactca
       61 atatattgca gagatcatgt aggcctgata agcgtagtgc atcaggcaat ttagcgtttg
      121 aaactgtttg tctgttcctt gccggatgcg acgtaaacgc cccatccggc atagcgaaac
      181 ttaaaattac tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac
      241 cggtttttcc gccgtagaat ggcggctatc gaggcagaag taaccttcac gctcaaactg
      301 gaatgcttta cccgcaaccg catctttcag cgacggttca gcaaagccct gtttgatcac
      361 cagcgattcc gggttaatca ccgacaggaa atcatccgca gcaccagggt tcggcacgct
      421 gaacagacgg tcgtacagac ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg
      481 aataacacct ttgactttac gaccatctgc cggatctttg cttaaggtgt cggcgtcata
      541 agtacagaag atggtggtga tattgccttc ggcatctttc tccacgcgtt ctgccttaat
      601 gacgtaagca ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt
      661 agcttcttcg cggaaatcgg cgcgatcgat ccaaatctca ccgctaaacg gcacctggcg
      721 gctacccatt tccggtttgt tcggatggtt cggcatggta accatttcgc cttcgccctg
      781 atagttttcg ataaccagtt tcaccggatc gataaccgcc attgcgcgcg gggcgttttc
      841 gttgagatct tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgctt
      901 ggtcacgccg atgcgtttgc agaactcacg aatagaagca gcagtgtaac cacgacgacg
      961 cagaccggaa atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac
     1021 cagcaggttc agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc
     1081 gtactggcgc gggtgaacag gaatggtgat gttgtccagc acccagtcgt acaggcgacg
     1141 gttgtcctgg aactcaagcg tacacagaga gtgcgtaata ccttccaggg catcgctgat
     1201 gcagtgggtg aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc
     1261 agcaaattta atgcggtaca gcaccggatc gcgcatcacg ataaacggtg aagccatgtc
     1321 gattttcgca cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa
     1381 cagcgccagg ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggttgcgt
     1441 cagggtgccg cggtattcgc ggatctgttc cggcgtcagt tcatcaacgt acgccaggcc
     1501 tttattgatc agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg
     1561 gacgttacca gaccagtgaa aacctaacca ctctacgtcg tttttgatcg actcaacata
     1621 ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata
     1681 gtcctgggcg atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc
     1741 attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc
     1801 atcgatgatc tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc
     1861 ctcaaagcgt aaacaacgta taacggcgta tgatcttata agcgggacag gctgacaact
     1921 gttagttagc gaaaatcgaa aatctcactg agattatggg gatgattgat gaaaaaaaaa
     1981 gcggcagaga agatctccac cgcttttgcc agtcccgcgt cattgttgga tgcgcgtctt
     2041 atccaaccag gactgagctt ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca
     2101 taaagcagat tactttttga tttcatacag cggtgtttga cccgccacaa cattgccctg
     2161 agctttaatg atcaagccac tgaaatcgtc gatattgctg caaaccaccg ggctaatcat
     2221 cgaacgggcg ttagcgttca ggtaatccag atccatttcc agaatcggtt gccctgcgct
     2281 cacctgcgcg ccctcttcca ccagacgttt aaagccttta ccttccagcg ctacggtgtc
     2341 gatacccatg tggacgacaa tctccgcgcc tttttcggtt tctaggcaga acgcgtggtt
     2401 ggtgttgaag attttcacga ttgtccctgc ggctggtgat acgacgattt tatctgtcgg
     2461 tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag aaacctgatc
     2521 cagtgccacg acatcaccgg taatcggcga taccagctcc gcgatagata ccgcgtttgg
     2581 tacagcctgc ggttttgcta caggagcggc agttgccgga gttgcttcag ctgacgcagc
     2641 tgctaccggg ccacgggcaa cgactttctt catcgcatcg ccaatggatt ctgctttcgc
     2701 gccaacaatc acctgaatgg tctgtttgtt cagttttact acaccagaag cacccagacg
     2761 tttacacatc gcatcgttga cgcgagctga gtcagccacg gtaaggcgca gacgggtaat
     2821 acaggcgtca atcgctttca ggttgtcagt accgccaacc gcagcaatat agttggttgc
     2881 cagttgagtc agaccttctt cagtattgct gttagcctct tcagttacga tctcgtcttc
     2941 tttatcttca cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac tgaacaccac
     3001 gaagtagata gcgaagaaga caacacccat caccagcagc atccagacgt tctggctggc
     3061 ggccggcagg ttatacatca acgcgtagtc gatagccccc gcagagaaag agaagccagc
     3121 atggataccc agcagcgttg ccacaaacag gctgataccg gtcagcagtg cgtgcaggag
     3181 gtacagcagc ggagcaagga acatgaacag gaattccagc ggctcagtca caccggtcag
     3241 gaacgcagta acagcaacag aaagcagcat cccgccaacc atcggacgac gctctttcgg
     3301 tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg aacatcatga tcgggaagaa
     3361 gccggacatg aacatccccg cggtgccgtc accagcgtag aaacggttga tgtcgccgtg
     3421 gaaaaccgta cccgccgcgt tggtgaattc accaatctgg aaccaggcga tggtgttcag
     3481 cacctgatgc agaccggttg ggatcagcag acggttgatg aaaccaaaga taccggaacc
     3541 cagcgcgcct gcagaaacga tccactcgcc gcctgcatgg atagcgtgct gtactggcgg
     3601 ccagacgtaa ccaaaaatgg ccgccagcac caggcagaag aagccggtgg caatcggcac
     3661 aaagcgtttg ccgccgaaga agctcaggaa gtccggcagt ttaatatcgg accaacggtt
     3721 ataggctgcg ccaccaacca gaccggtaat gatacccgcc agtacaccca tgttaatttc
     3781 tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac ccgccagcgc
     3841 cgccgcacct gcgctgtctt tcgaccagct ggatgccaca ccgatggcga agattaatgc
     3901 gaggttatca aaaatcgcac cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg
     3961 ctgaccgaat cgcagcaaca gtgccgccac cggcagcacc gcgataggga gctgtaacgc
     4021 cctaccgagt cgctggaaaa aacctaaaat attcatctta ttccccctac gagaacccta
     4081 tttggctcgt ttaaagccgt atttttattt tgctgcaaat tgtactgccg atgttctgta
     4141 atcagattgt tagatcatct gctacagagt gtgtgaaaat ttaattcgta tcgcaaatta
     4201 aacgcgtgcc ttttgtgagt tttgtcacca aatatcgtta tcatcactcc cttttactgg
     4261 ctaaagcaga aaacttattt tatcattcaa aaaatcaggt cggattgacg cctgtctgcg
     4321 caaatccagg ttacgcttaa agatgcctaa tccgccaacg gcttacattt tacttattga
     4381 ggtgaataat gagactgatc cccctaacta ccgctgaaca ggtcggcaaa tgggctgctc
     4441 gccatatcgt caatcgtatc aatgcgttca aaccgactgc cgatcgtccg tttgttctgg
     4501 gcctgccgac tggcggcacg ccgatgacca cctataaagc gttagtcgaa atgcataaag
     4561 caggccaggt cagctttaag cacgttgtca ccttcaacat ggacgaatat gtcggtctgc
     4621 cgaaagagca tccggaaagc tactacagct ttatgcaccg taatttcttc gatcacgttg
     4681 atattccagc agaaaacatc aaccttctca acggcaacgc cccggatatc gacgccgaat
     4741 gccgccagta tgaagaaaaa atccgttctt acggaaaaat tcatctgttt atgggcggtg
     4801 taggtaacga cggtcatatt gcatttaacg aaccggcgtc ttctctggct tctcgtactc
     4861 gtatcaaaac cctgactcat gacactcgcg tcgcgaactc tcgtttcttt gataacgatg
     4921 tcaatcaggt gccaaaatat gccctgactg tcggtgttgg tacattgctg gatgccgaag
     4981 aagtgatgat tctggtgctg ggtagccaga aagcactggc actgcaggct gcagttgaag
     5041 gttgcgtgaa ccacatgtgg accatcagct gtctgcaact gcatccaaaa gcgatcatgg
     5101 tgtgcgatga accttccacc atggagctga aagttaagac tttaagatat ttcaatgaat
     5161 tagaagcaga aaatatcaaa ggtctgtaat tgttatccct gccctggctc cttgctcagg
     5221 gcaatatttt ttaaaatcgg ggggcagaat gtatgcatta acccagggcc ggatctttac
     5281 cggccacgaa tttcttgatg accacgcggt tgttatcgct gatggcctga ttaaaagcgt
     5341 ctgtccggta gcggaactgc cgccagagat cgaacaacgt tcactgaacg gggccattct
     5401 ctcccccggt tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt ttaacgacac
     5461 cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg agaaatcagg
     5521 ctgtactaac tatctgccga cgcttatcac caccagcgat gagctgatga aacagggcgt
     5581 gcgcgttatg cgcgagtacc tggcaagaca tccgaatcag gcgttaggtc tgcatctgga
     5641 aggtccgtgg ctgaatctgg taaaaaaagg cacccataat ccgaattttg tgcgtaagcc
     5701 tgatgccgcg ctggtcgatt tcctgtgtga aaacgccgac gtcattacca aagtgaccct
     5761 ggcaccggaa atggttcctg cggaagtcat cagcaaactg gcaaatgccg ggattgtggt
     5821 ttctgccggt cactccaacg cgacgttgaa agaagcgaaa gccggtttcc gcgcggggat
     5881 tacctttgcc acccatctgt acaacgcgat gccgtatatt accggtcgtg aaccgggcct
     5941 ggcgggcgcg atcctcgacg aagctgacat ttattgcggt attatcgctg atggcctgca
     6001 tgttgattac gccaacattc gtaacgctaa acgccttaaa ggcgacaaac tgtgtctggt
     6061 taccgacgcc accgcgccag caggtgccaa cattgaacag ttcatttttg cgggtaaaac
     6121 aatatactac cgtaacggac tttgtgtgga tgagaacggt acgttaagcg gttcatcctt
     6181 aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac tggatgaagt
     6241 gctgcgtatg gcgacgctct atccggcgcg tgcgattggc gttgagaaac gtctcggcac
     6301 actcgccgca ggtaaagtag ccaacctgac ctcattcaca cctgatttta aaatcaccaa
     6361 gaccatcgtt aacggtaacg aggtcgtaac tcaataagag aaagtatgac accaggcgga
     6421 caagctcaga taggtaatgt tgatctcgta aaacagctta acagcgcggc ggtttatcgc
     6481 ctgattgacc agtacgggcc aatctcgcgg attcagattg ccgagcaaag ccagcttgcc
     6541 cccgccagcg taaccaaaat tacgcgtcag cttatcgaac gcgggctgat caaagaagtt
     6601 gatcagcagg cctccaccgg gggccgccgc gctatctcca tcgtcaccga aacccgcaat
     6661 ttccacgcaa tcggcgtacg gcttggtcgt cacgacgcca ccatcaccct gtttgatctc
     6721 agcagcaaag tgctggcaga agaacattac ccgctgccgg aacgtaccca gcaaacgctg
     6781 gaacatgccc tgttgaatgc cattgctcag tttattgata gctaccagcg caaactgcgc
     6841 gagctgatcg cgatttcggt gatcctgcca gggcttgttg acccggacag cggcaaaatt
     6901 cattacatgc cgcatattca ggtagaaaac tgggggctgg tagaagctct ggaagaacgt
     6961 tttaaagtga cctgtttcgt tggtcacgat atccgtagtc tggcgctggc ggagcactac
     7021 ttcggtgcaa gtcaggattg cgaagactcc attctggtgc gtgtccatcg cggaaccggg
     7081 gccgggatta tctctaacgg gcgcattttt attggccgca acggtaacgt cggtgaaatt
     7141 ggccatattc aggtcgaacc gctgggtgaa cgctgccact gcggcaactt tggctgcctg
     7201 gaaactatcg ccgccaacgc tgccattgaa caacgggtgt tgaatctgtt aaagcagggc
     7261 taccagagcc gcgtgccgct ggacgactgc accatcaaaa ctatctgcaa agccgcgaac
     7321 aaaggcgata gtctggcgtc ggaagtaatt gagtatgtcg gtcgtcatct gggtaaaacc
     7381 atcgccattg ctatcaactt atttaatccg caaaaaattg ttattgccgg tgaaatcacc
     7441 gaagccgata aagtgctgct ccctgctatt gaaagctgca ttaataccca ggcgctgaag
     7501 gcgtttcgta ctaatctgcc ggtggtacgt tctgagctgg atcaccgctc ggcaatcggc
     7561 gcttttgcgc tggtaaaacg cgccatgctc aacggtattt tgctccagca tttgctggaa
     7621 aattaatgtg cttttatagt ggcgcttatt gttgtcaata ttctgggtag tccatgacca
     7681 ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca cgataacgtc gccgtaccgg
     7741 gtgcagcgga atttttgcac gggattatgg ataaaggcct gccgctggtg ttgctgacca
     7801 actatccttc gcagaccggg caagatctgg cgaaccgctt tgccactgcc ggtgtcgatg
     7861 tgccggatag cgtgttttat acctctgcga tggcgaccgc cgatttcctg cgtcgccagg
     7921 aaggcaagaa agcatatgtg gtgggcgaag gcgcactgat tcatgaactg tacaaagccg
     7981 gtttcactat taccgatgtg aaccctgatt tcgtgattgt tggcgaaacg cgttcctaca
     8041 actgggacat gatgcataaa gcggcctatt tcgtcgctaa cggtgcacgc tttatcgcca
     8101 ccaacccgga cacccacggg cgcggttttt atcccgcttg tggcgcgttg tgtgcaggga
     8161 ttgagaaaat ctccgggcgc aaaccgttct atgttggtaa gcccagcccg tggatcatcc
     8221 gcgcagcatt aaacaaaatg caggcgcatt cggaagaaac ggtgattgtc ggcgataact
     8281 tgcgcaccga cattctggcg ggcttccagg caggtctgga aacgattctg gtgctttctg
     8341 gtgtttcatc gctcgacgat atcgacagta tgccgttccg cccaagctgg atttacccgt
     8401 cagtcgctga aatcgacgtt atctgaatac cacccacgtc tgcggacgtg gttggctctg
     8461 atgacaaacg ccaaactgcc tgatgcgcta cgcttatcag gcctacgcag ctcctgcaat
     8521 atattgaatt ttcatgcttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
     8581 caaatcgcac tttatcagca atttggcc
//
LOCUS       DAZRUF010000173         8571 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZRUF010000173 DAZRUF010000000
VERSION     DAZRUF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8571
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.173"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            308..7373
                     /gene="yeeJ"
                     /locus_tag="ACHXOL_003170"
                     /pseudo
     CDS             308..7373
                     /gene="yeeJ"
                     /locus_tag="ACHXOL_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(7635..>8571)
                     /locus_tag="ACHXOL_003171"
     CDS             complement(7635..>8571)
                     /locus_tag="ACHXOL_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR6810086.1"
                     /translation="DAMAIFPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTL
                     VTMTMQDHATLKEIKDLAKNITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVV
                     GITMNCYSPLCKNASRMDQKLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVR
                     EEMRSLGSYIAFNLEGSSQERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARAL
                     VDCYNNVYRLSLPPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRW
                     PAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         2430 a         2082 c         2039 g         2020 t
ORIGIN      
        1 aaaaattcgc ttttttagcg caatgtcact gaccttagtt gaacattgtt ttttaacgga
       61 tagcgggttt ttaacacctt aagcgccctc gacctttatg gttgagggcg ttttgctatg
      121 aacgccatca ccattttccc ctcgattata aaacttgagt tattcagtag tctcccctct
      181 tgcaactcac acccaaaact gcctaacgaa aagttattaa ttttcaatcg tattgctatc
      241 agtatttaca ttttttcgct gtgctagaaa gggcgcattt atgttagctc gttcagggaa
      301 ggtaagcatg gctacgaaga agagaagtgg agaagaaata aatgaccgac aaatattatg
      361 cgggatggga attaaactac gccgcttaac tgcgggtatc tgtctgataa ctcaacttgc
      421 gttccctatg gctgcggcag cacaaggtgt ggtaaacgcc gcaacccaac aaccagttcc
      481 tgcacaaatt gccattgcaa atgccaatac ggtgccctac acccttggag cgctggaatc
      541 ggcccaaagc gttgccgaac gtttcggtat ttcggtggct gagttacgca aactcaacca
      601 gtttcgtacg tttgctcgag gttttgataa tgtccgccag ggtgatgaac tggatgtccc
      661 ggcacaagtt agtgaaaaaa aattaacccc gccgccgggt aatagcagtg acaacctcga
      721 gcaacagata gccagtactt cacagcaaat cgggtctctg ctcgccgaag atatgaacag
      781 cgagcaagcg gcaaatatgg cgcgtggatg ggcctcttct caggcttcag gcgcaatgac
      841 agactggtta agccgcttcg gtaccgcaag aatcacgctg ggcgtggatg aagattttag
      901 cctgaagaac tcccagttcg attttctcca tccgtggtat gaaacgcctg ataatctctt
      961 tttcagtcag catactctcc atcgtactga cgagcgtacg cagattaaca acggcttagg
     1021 ttggcgtcat ttcactccca catggatgtc gggcatcaac ttctttttcg accacgatct
     1081 tagccgttac cactcccgcg ccggcattgg cgcggagtac tggcgcgact atctaaaatt
     1141 aagcagtaac ggctatttgc gactgaccaa ctggcgcagc gcacctgaac tggacaacga
     1201 ttataaagca cgcccggcca atggctggga tgtacgcgca gaaagctggc tacccgcctg
     1261 gccgcacctt ggcggtaaac tggtctatga acagtattat ggcgatgaag tggccctgtt
     1321 cgataaagac gatcggcaaa gtaatcctca tgccataacc gctggactta actatacccc
     1381 cttcccgctg atgaccttca gcgcggagca acgccagggt aaacagggcg aaaatgacac
     1441 ccgttttgcc gtcgatttta cctggcaacc tggcagcgca atgcagaaac agcttgaccc
     1501 gaatgaagtc gctgcacggc gtagccttgc aggcagccgt tatgatctgg tggatcgcaa
     1561 caacaatatc gttctggaat atcgcaaaaa agaactggtt cgcctaaccc tgacagaccc
     1621 cgtgacaggg aagtcaggag aagtgaaatc actggtttcg tcgctacaaa ccaaatatgc
     1681 cctgaaaggc tataacgtcg aagccaccgc actggaagct gccggtggca aagtggtcac
     1741 aacgggtaaa gatattctgg ttaccctgcc ggcttaccgg ttcaccagta cgccagaaac
     1801 cgataacacc tggccgattg aagtcaccgc cgaagatgtc aaaggcaatt tgtcgaatcg
     1861 tgaacagagc atggtggtcg ttcaggcacc tacgctaagc cagaaagatt cctcggtatc
     1921 gttaagtacc caaacattga acgcggattc ccattcaacc gccacactga cttttattgc
     1981 gcatgatgca gcaggtaatc ctgttgtcgg gctggtgctc tcgacgcgtc acgaaggtgt
     2041 tcaggacatc accctttctg actggaaaga taatggtgac ggaagctata cccagatcct
     2101 gaccacagga gcgatgtctg gcacgctgac gctgatgcca cagctgaatg gtgtggatgc
     2161 ggctaaagcc cccgccgtgg tgaatatcat ttctgtttcg tcatcccgaa ctcactcgtc
     2221 aattaagatt gataaggacc gttatctctc cggcaatcct atcgaggtga cggtagaact
     2281 gagagatgaa aatgacaaac ctgttaagga acaaaaacag caactgaata acgcagtcag
     2341 catcgacaac gtgaaaccag gagtcactac agactggaaa gaaaccgcag atggcgtcta
     2401 taaggcgacc tataccgcct ataccaaagg cagtggactt actgcgaagc tattggaatg
     2461 aagatttgca taccgctgga tttatcatcg acgccaaccc gcagtcagcg aaaattgcga
     2521 cattatctgc cagcaataat ggtgtactcg ccaatgagaa tgcagcaaac accgtctcgg
     2581 tcaatgtcgc tgatgaagga agcaacccaa tcaatgatca taccgtcacg tttgcggtat
     2641 taagcggatc ggcaacttcc ttcaacaatc aaaacaccgc aaaaacggat gttaatggtc
     2701 tggcgacttt tgatctgaaa agtagtaagc aggaagacaa cacggttgaa gtcacccttg
     2761 aaaatggcgt gaaacaaacg ttaatcgtca gttttgtcgg cgactcgagt accgcgcagg
     2821 ttgatctgca gaagtcgaaa aatgaagtgg ttgctgacgg caatgacagc gccacaatga
     2881 ccgcgaccgt ccgggatgca aaaggcaacc tgctcaatga cgtcatggtc actttcaatg
     2941 ttaattcagc agaggcgaaa ctgagccaaa ccgaagtgaa tagccacgac gggatcgcca
     3001 cagctacgct gaccagtttg aaaaatggtg attatagggt tacggcctct gtgagctctg
     3061 gttcccaggc taatcaacag gtgaatttta tcggtgatca aagtactgct gccctgaccc
     3121 tcagtgtgcc ttcaggtgat atcaccgtca ccaacacagc tccgcaacat atgactgcaa
     3181 ccttgcagga taaaaatggc aacccactaa aagataaaga aatcaccttc tctgtgccaa
     3241 acgacgtcgc aagtaagttc tcgattagca acggaggaaa aggcatgacg gatagtaacg
     3301 gggttgcaat cgcctccctg accggcacgt tagcgggcac gcatatgatc atggctcgtc
     3361 tggctaacag caatgtcagc gatgcacagc caatgacgtt tgtggcggat aaagacagag
     3421 cggttgtcgt tttgcaaaca tcgaaagcgg aaatcattgg gaatggcgtg gatgagacaa
     3481 ctctgacagc aacagtgaaa gatccgtcga atcatccggt ggcggggata acggtaaact
     3541 tcaccatgcc acaggacgtt gcggcaaact ttacccttga aaataacggt attgccatca
     3601 ctcaggccaa tggggaagcg catgtcacgc tgaaaggtaa aaaagcgggc acgcatacgg
     3661 ttaccgcaac gctgggtaat aacaatacca gtgattcgca gccggtaaca tttgtggcgg
     3721 acaaagcctc ggctcaggtt gtcctgcaga tatcaaaaga tgagatcaca ggtaatggcg
     3781 tcgatagcgc aacgctaact gcaacggtta aagatcagtt cgacaatgag gtgaataatc
     3841 ttccggtaac attcagctca gcctcttcag gactcaccct gaccccggga gtaagtaata
     3901 ccaatgagtc tggcatcgcg caggccactc tcgcaggcgt tgcctttggt gagcagacgg
     3961 tcactgcatc actggctaat aatggtgcca gcgacaacaa aactgtgcat tttattggcg
     4021 acacagcggc ggcaaaaatt atcgagttga cgcctgtccc agacagcata atcgccggta
     4081 ccccgcagaa cagctccggc agcgccatca ccgccacagt cgttgataat aatggctttc
     4141 cggtgaaagg tgtgactgtg aacttcacca gcagaacaaa ctctgccgaa atgacgaatg
     4201 gcggccaagc cgtaacgaac gaacagggta aggctaccgt cacttatacc aatacccgct
     4261 cctcgataga atcaggagcg agaccggata ccgttgaggc cagtctggaa aatggtagct
     4321 ccacgcttag cacatcaatt aatgtcaacg ctgatgcgtc tacggcacat ctcacctggc
     4381 tacaggcact ttttgataca gtctccgcag gcgagacaac cagtctgtat attgaggtga
     4441 aggataatta cggcaacggt gtcccccagc atcaggtgac cctcagcgtt tcaccaagtg
     4501 aaggcgtgac ccccagtaat aacgctatat atactaccaa ccacgacggc aatttttacg
     4561 caagctttac cgctacaaaa gccggggttt atcaagtgac ggcaaccctc gaaaatggcg
     4621 attcgatgca acaaacagtg acctatgtgc cgaacgtcac gaatgccgaa atcacgctgg
     4681 caacctcgaa ggatccggtg attgccgaca ataacgatct cacgacacta acagcaacag
     4741 tcgctgatac agagggcaat gcgatagcca acactgaagt aacatttact ctgccggaag
     4801 atgttaaggc gaacttcacg ctgagcgatg gcggtaaagc gattactgat gctgaaggca
     4861 aagcgaaagt cacgctgaaa ggtacaaaag caggcgctca tactgttaca gcatcgataa
     4921 ctggcggtaa gagtgagcag ttggtggtga actttactgc ggatacgctc actgcgcagg
     4981 ttaatcttaa cgttaccgag gacaatttta tcgctaataa cgtcgggatg accaggctgc
     5041 aggcaacagt gactgatgga aacggcaacc cgttagccaa tgaggcggtg acattcacgc
     5101 taccggcaga tgtgagcgca agctttactc tcggacaagg cggttccgcc attactgaca
     5161 tcaacggcaa ggctgaagtt acactgagcg gtacaaaatc cggcacctac cccgtgacag
     5221 ttagcgtgaa caattatggt gtcagtgata cgaaacaggt gactttgatt gccgatgctg
     5281 gtaccgcaac actagcctcc ttaacctctg tatactcatt cgtcgtcagc acgaccgagg
     5341 gcgcgaccat gactgcaagc gtcactgacg ctaacggcaa cccggtagaa ggcataaaag
     5401 ttaatttccg cggaacctcc gtcacgctaa gcagcaccag cgttgaaacg gatgatcggg
     5461 gtttcactga aattcttgtg acaagcaccg aagtcggact gaaaacagtt tcagcctctc
     5521 tggcagataa acctactgaa gtcatatcgc gattactgaa tgcaaaagca gatattaatt
     5581 ctgcaacgat taccagtctg gagatacctg aaggtcaggt catggtcgca caagacgtag
     5641 cagttaaagc tcacgtcaac gaccagtttg gcaacccgat tcttaatgaa tctgtaacat
     5701 tcagtgcaga gccaccagag cacatgacca tcagccaaaa tattgtctct actgatacgc
     5761 atggtatagc cgaggtctcc atgacgcccg aaagaaacgg ttcgtatatg gtgaaagcat
     5821 ccctggcgaa tggagcctca cttgagaaac aactggaggc tattgatgaa aaactgacac
     5881 tctcggcgtc cagcccgctt atcggtgtca attccccaac aggtgcaact ctgacggcaa
     5941 cgctaacttc tgcaaatggc actccagtgg agggtcaggt catcaacttt agcgtaacgc
     6001 cagaaggtgc gacgttaagt ggcggaaaag tgagaactaa ctcttctggt caggctccag
     6061 tcgttctgac cagcaataaa gtcggtacat atacggtgac tgcatcgttc cataacggcg
     6121 taacaataca gacacagaca accgtgaaag tcactggcaa ctcaagcacc gcccatgtta
     6181 ctagctttat cgctgatcca tcgactatcg ccgccaccaa cagtgattta agtaccttaa
     6241 aggcaacggt tgaggatggc agtggtaacc tgatcgaagg tctcactgtg tactttgcct
     6301 taaaaagcgg ctctgccaca ttaacgtcat taacagcggt gaccgatcaa aacggaatcg
     6361 cgacaacaag cgtgaaagga gcgatgacag gtagcgtcac ggtaagcgca gtcacgaccg
     6421 ctggtggaat gcaaacagta gatataacgc tggtggcagg cccagcagac gcctcaaagt
     6481 cagtccttaa gaacaatcgg tcatcattga aaggagactt taccgatagt gctgagctac
     6541 atcttgttct gcacgatata tcaggcaatc cgatcaaagt ttctgaaggg ctggaatttg
     6601 tacagtcagg taccaacgtg ccctatgtgc aagttagtgc aattgactac agtaaaaatt
     6661 tctcaggcga gtacaaagcc actgttacag gaggcggaga gggtatcaca acgctgatcc
     6721 ctgtattgaa tggtgttcat caagctggtc tgagtaccac aatacaattc actcgcgcag
     6781 aagacaaaat aatgagcggt acagtatcag tcaatggtac tgacctaccg acaactacat
     6841 tcccttcgca ggggttcacc ggggcgtatt atcagttgaa taatgacaac tttgccccag
     6901 gaaaaacggc ggctgattat gagttttcaa gctctgcctc ctgggtcgat gttgatgcta
     6961 ccggtaaagt gacatttaaa aatgtcggca gcaattcgga aaggattacg gcgacaccaa
     7021 aatcaggagg ccctagctat gtatacgaaa tccgtgtgaa gagttggtgg gtgaacgccg
     7081 gcgaggcttt catgatatac agccttgctg aaaatttttg cagcagcaat ggctacacgc
     7141 tccccagagc aaactattta aaccacagta gttcccgagg catcgggtca ctgtacagtg
     7201 aatggggaga tatggggcat tacacgactg aagctggttt tcaatcaaat atgtattggt
     7261 catctagtcc cgcaaactca agcgaacaat acgtagtttc cctggcaaca ggtgatcaaa
     7321 gcgtatttga aaagcttggg tttgcttatg cgacatgtta taaaaacctc tgattttccg
     7381 ttataatttc ttaaagagtg ctccattcgg agctctcttt tttacattcc ctctatagag
     7441 cttgcccttc tctcacagaa aatgaataaa taaaaatgcg gcaccgccag aattgcgttc
     7501 gatgtgacta tgatcgtatc cggtgagtac tgctttgatt caaaggctgg ttttctgtga
     7561 tgattgacca gcctcagagt attgatgaca ccaccgttca taacagatcg gcattactat
     7621 gcataagtac tgtctcatat tctcgccaaa acacttttta atgcctttgc gaattcatcc
     7681 agacttatat tgtcaatttt ttgcccaacg acgactgact ccgagacatc ggccattgcg
     7741 ggccatcgcg ttttgtaatc agcataaatt gcaacaacag gggtattata ggcacttgcc
     7801 atatgtaata ttgaggtgtc aggagttatt gcgatataag catcttttat gattgctgct
     7861 gaacgtttaa tcgagggtgg taaggataaa cggtagacat tattataaca atccactaat
     7921 gccctggctt tatcttcacc tttgggtcca tgaacgatca ctattggtat atctgtttca
     7981 ctttgaattt ttgctattag attttctgca atcgataatg aaaatgtacg ttcctgcgag
     8041 ctaccttcta aattgaacgc aatgtaagat cctaacgatc gcatttcctc gcgcacctca
     8101 tcgagtacat cctcacttag tggcaattca tatattggcc tgactgctgg aaaacccgcc
     8161 tcacgcatca taaatgccca tgtcataggt acgggagccc ggagtttctg atccatacgg
     8221 gacgcgttct tgcacaaagg ggagtagcaa ttcatggtta taccaacaac ctgaaaattc
     8281 gtttttgctt tcaactgact gataaataac atcgttttca ggttcttttt acgcatcccc
     8341 tcaatacaaa gatccggcgt accgtattgc tgtgttatgt tctttgctaa atcttttatt
     8401 tcttttaatg ttgcgtgatc ctgcatagtc attgtgacta atgttaattt agtctgttca
     8461 agtttaagcg cattaaagac ttctaaatta attgtcgacg ttacaattaa aagatgctta
     8521 attttatgca attcaagcgc ccgaataaca ggaaagatgg ccatagcatc g
//
LOCUS       DAZRUF010000174         8553 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZRUF010000174 DAZRUF010000000
VERSION     DAZRUF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.174"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            27..299
                     /gene="rcnR"
                     /locus_tag="ACHXOL_003172"
     CDS             27..299
                     /gene="rcnR"
                     /locus_tag="ACHXOL_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR6810087.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      391..487
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            522..1310
                     /gene="thiM"
                     /locus_tag="ACHXOL_003173"
     CDS             522..1310
                     /gene="thiM"
                     /locus_tag="ACHXOL_003173"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR6810088.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            1307..2107
                     /gene="thiD"
                     /locus_tag="ACHXOL_003174"
     CDS             1307..2107
                     /gene="thiD"
                     /locus_tag="ACHXOL_003174"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR6810089.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            2172..2990
                     /locus_tag="ACHXOL_003175"
     CDS             2172..2990
                     /locus_tag="ACHXOL_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR6810090.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            3042..3788
                     /locus_tag="ACHXOL_003176"
     CDS             3042..3788
                     /locus_tag="ACHXOL_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR6810091.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(3762..4727)
                     /locus_tag="ACHXOL_003177"
     CDS             complement(3762..4727)
                     /locus_tag="ACHXOL_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR6810092.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4724..5728)
                     /locus_tag="ACHXOL_003178"
     CDS             complement(4724..5728)
                     /locus_tag="ACHXOL_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR6810093.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(5725..7002)
                     /locus_tag="ACHXOL_003179"
     CDS             complement(5725..7002)
                     /locus_tag="ACHXOL_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR6810094.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            7259..8311
                     /gene="fbaB"
                     /locus_tag="ACHXOL_003180"
     CDS             7259..8311
                     /gene="fbaB"
                     /locus_tag="ACHXOL_003180"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR6810095.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
BASE COUNT         2152 a         2221 c         2238 g         1942 t
ORIGIN      
        1 catattcttg ttaaccaggt gttgccatgt ctcatacaat ccgtgataaa cagaaactga
       61 aagcgcgtgc cagtaagatt cagggccagg tcgtggcgct caagaaaatg ctcgacgagc
      121 cgcatgaatg cgctgcagtt ttacaacaga ttgctgctat ccgtggcgcg gtaaacggtc
      181 tgatgcggga agtgattaaa ggtcatctga cggaacacat cgttcaccag ggggatgagc
      241 taaaacgtga agaagatctg gatgtcgttc tgaaggtgct ggattcatat atcaaataat
      301 ttattaacgc gattgtaaaa ctgccgtttt tcctcgttta caacgcgtgc gctggacatt
      361 accatcctcc tctgcgattt atcatcgcaa ccaaacgact cggggtgccc ttctgcgtga
      421 aggctgagaa atacccgtat cacctgatct ggataatgcc agcgtaggga agtcacggac
      481 caccaggtca ttgcttcttc acgttatggc aggagcaaac tatgcaagtc gacctgctgg
      541 gttcagcgca atctgcgcac gcgttacacc tttttcacca acattcccct cttgtgcact
      601 gcatgaccaa tgatgtggtg caaaccttta ccgctaatac cttgctggcg ctcggtgcat
      661 cgccagcgat ggttatcgaa accgaagagg ccagtcagtt tgcggctatc gccagtgcct
      721 tgttgattaa cgttggcaca ctgacgcagc cacgcgctca ggcgatgcgt gctgccgttg
      781 agcaagcaaa aagctctcaa acaccctgga cgcttgatcc agtagcggtt ggtgcgctcg
      841 attatcgccg ccatttttgt catgaacttt tatcttttaa accggcagcg atacgtggta
      901 atgcttcgga aatcatggca ttagctggca ttgctaatgg tggacgggga gtggatacca
      961 ctgacgccgc agctaacgcg atacccgctg cacaaacact ggcacgggaa actggcgcaa
     1021 tcgtcgtggt cactggcgag gtggattatg ttaccgatgg ccatcgcgcc gttggcattc
     1081 acggtggcga tccgttaatg accaaagtgg taggaactgg ctgtgcatta tcggcggttg
     1141 tcgctgcctg ctgtgcgtta ccaggcgata cgctggaaaa tgtcgcatct gcctgtcact
     1201 ggatgaaaca agccggagag cgcgcagtcg ccagaagcga ggggctaggc agttttgttc
     1261 cacatttcct tgatgcgctc tggcaattga cgcaggaggt gcaggcatga aacgaattaa
     1321 cgctctgacg attgccggta ctgatccgag tggtggtgcg gggattcagg ccgatcttaa
     1381 aaccttctcg gcacttggcg cttatggttg ctcagttatt actgcactgg tggcgcaaaa
     1441 tacccgtggc gtacagtcgg tgtatcgcat tgagcctgat tttgtcgccg cccagctcga
     1501 ttcggtgttc agcgatgtgc gaatcgatac cactaaaatc ggtatgttgg cggaaaccga
     1561 tattgttgaa gcggtggcag aacggttgca acgttatcag atccaaaacg tggtactcga
     1621 caccgttatg ctggcaaaaa gcggcgaccc actgctttca ccttcggcag ttgccacgct
     1681 acgcagtcga ttattgccac aggtttcatt aataacgcca aacttgcccg aagcagccgc
     1741 cttgctcgac gcgccacacg cgcgcaccga acaggaaatg ctggaacaag ggcgatcgct
     1801 gttggcgatg ggctgtggcg cagtgctaat gaaaggtggt catctggatg atgagcaaag
     1861 cccggactgg ctgtttaccc gcgagggtga acaacggttt accgcaccgc gcattatgac
     1921 caaaaacacc cacggtactg gttgtacact ctctgcggcg ttggctgcac tacgcccgcg
     1981 ccatacaaac tgggctgaca ccgtacagga ggcaaaaagc tggctttcat cggcgttagc
     2041 ccaggccgac acgctggaag ttggtcacgg tattggtccg gttcaccact tccacgcctg
     2101 gtggtgagca ccgtactata gctgctttgg ttattgtcag cctttggcac tgacattcag
     2161 ggatgaaacc aatgcagcta agaattacca gtcgaaaaaa actcaccgct ctattatgcg
     2221 ctcttgggct aatttccatc gttgcaattt atccgcgtca gacggtgaat tttttctatt
     2281 cgacagcagt tcagattaca gactacatcc acttctacgg ttatcgcccg gttaaatctt
     2341 tcgctattcg tattcctgcc agttacacca ttcacggaat agatgtttcg cgctggcagg
     2401 agcggatcga ctggcagcgt gtggcaaaaa tgcgcgataa cggtattcgc ttacaatttg
     2461 cctttattaa ggcgacggaa ggcgaaaagc tggtggaccc ctatttttcg cgtaactggc
     2521 aactaagccg cgaaaatggc ttgctgcgcg gggcatatca ttatttctcc ccgtcggtag
     2581 cagcaccggt tcaggtgcga ttatttctgc aaacggtaga tttctcacaa ggcgatttcc
     2641 ctgcggtact ggacgtagaa gagcgaggaa aattatcggc aaaagaatta cgcaagaggg
     2701 taagtcagtg gctaaaaatg gtcgaaaaaa gtacggggaa aaagccgatt atttactcag
     2761 gagccgtttt ttatcacacg aatctggcgg gctatttcaa tgaatatccg tggtgggtgg
     2821 ctcactacta tcaacgtcgt ccggacaatg acggcatggc ctggcgcttc tggcagcatt
     2881 ccgaccgtgg acaggtagat ggcatcaatg gtccggtgga ttttaatgtg tttaatggca
     2941 cggtggagga gttgcaggga ttcgttgatg ggattaaaga aacgccttaa ataagaagca
     3001 gtgatatact cccaccagac tccctggcag gaaaaagaca aatggaacaa gcgcataccc
     3061 agctgattgc ccaactgaac gaacgtattt tagcggcgga taacacgccg ctttatatta
     3121 agtttgccga aacggtaaaa aatgccgtgc gcagcggggt gctggagcat ggcaatattt
     3181 tgcccggtga gcgtgattta agtcagttaa ccggcgtttc gcgcattacg gtgcgcaagg
     3241 cgatgcaggc gctggaagaa gagggcgtgg tgacgcgttc gcgcggttac ggcacgcaga
     3301 tcaacaacat cttcgaatac tcgttgaaag aagcgcgagg tttttctcag caggtggtgt
     3361 tgcgcgggaa aaagcccgat acgctatggg ttaacaagcg tgtcgtgaaa tgtcccgaag
     3421 aagtcgcgca gcagctggcg gtcgaagcag gaagtgatgt ctttttgctt aagcgtattc
     3481 gctatgtcga tgaagaagcg gtatcgattg aggaatcgtg ggttccggcg catttaattc
     3541 atgatgttga tgccatcggg atttcacttt atgactattt ccgcagccag cacatttacc
     3601 cacaacgtac gcgttcccgc gttagcgccc ggatgccgga tgccgagttt cagtcacata
     3661 ttcagttaga tagcaaaata ccggtgctgg tgatcaagca agtggcgctt gaccaacagc
     3721 aacggcctat tgagtacagc attagccact gtcgcagcga tctatacgtt tttgtgtgcg
     3781 aggagtagtt cctcgcgcgt tggcgcacaa tcaccgcccc gatgcccgac aacccacgac
     3841 gccactgcat tgcccagtaa tacggcatcc gccagtggca gaccagaggc cagcccggca
     3901 agtacgccac ctgcatgact gtcgcccgcc ccaatggtgt ctacaacttg cgttggaaat
     3961 gccggaatgc agccagaagc gtcgttgctg aaataccatg cgccttcttt atcgagacga
     4021 acgatcaacg gcgcggcaaa tttctcctgc cattgctcgc caagtgttgt tatctctgcg
     4081 gataaagcaa aacgttcggc ggcaatctca gcctcttgac gattgagcga cactaaaggt
     4141 cgacaggcca tgatccgcgc cagtaatgca tccgggatat cgccaatacg tgggccaaaa
     4201 tcgataaacg gcgtcacgtc ttgcagctct tccagccatt ccactaacaa ttcgccgcag
     4261 ggcgaggcca gttgataacc ggaaaaatag agcaggctgc caggcgcaac ggttaatcgc
     4321 gccagccact ggcgattcca ctgattttcc acaccgctga atgacataaa agtgcgttcg
     4381 ccatccggct caaccagcgc cagacaccag ccgttatcac cttcggcgtt atcgatcagg
     4441 ctgattaagc cctcttttgc catccggttg cgaataatct ccgcccacac gccctgaccg
     4501 agcggcaagg cattacccgc ttcgatgccg aggcgcttta acgccacggc aatattcagt
     4561 gcgcagccgc caacgttaac gctctgctgt ttcagttcga tatcgcaccc acgccagggg
     4621 agggcataag cgtcggcgat cacgtcaatg accgccgcgc cgacgaccat caccggctga
     4681 cgcgtggtta attcaggcag cagcgtgtgt aatcgagcgc cgctcatacc gcctcccgtt
     4741 gttgacgata tttcgccagc gctgtggcat agcggttgaa atcaagctga tttaccgcat
     4801 ccagttccgc ctttaacgca ggatcgatag cgttaacgcc atgcaacgcg ccgcaaattg
     4861 ccgtcgccat agcaccgatg gtgtctgtgt cgccgccaag gttagcgcac aggacggcgc
     4921 agcgattcgg gtcggtttgt gctagttcaa ccagcgcaat ggcgcacgga acggactcaa
     4981 tagtgctggt acctgcgcca acgacctggt aaagctgttc gctggcggat tcggtgccgt
     5041 cggcattgcg cacaattttc agcgcaatct ccagccgcgc cgccagtgag gcgctgaacg
     5101 tagtgatgcg tttttgctgt gcatgtcgcg caattgaagg cagggaatca acaatcgctg
     5161 accagctttc tccgtcaatg gcacgagaaa tcgcccatgc gatgactacc gcgcctgcaa
     5221 ccgccagatc ggatttatgg gtcgggctgg acgccagcgc tacatcatca ataaaggaat
     5281 caacatcacg cgccggaagc aaacaaccta atggcgagac gcgcatcgct gcgccgttgg
     5341 tcacgccgtt gttttccagt tcagcaacgg gtttaccgtc gcgaatggcg ttaagcgcaa
     5401 tcttcgaggt cggacctagt acgtttttgt taaaggcgtc gaaacgcagc gcccagtcga
     5461 gaatattacg cccaatcaga tccggatcga tcttgccttc acgttccagt aacgcatccg
     5521 ccagacacag cgccatcgag gtatcgtcgg tgaattcggc gcggttaaaa taacaggccg
     5581 cgttattctc ctttggtcca ggaagaaaac ggtcaatcca gccaaagtgt gctttaacgc
     5641 ggctgcgtgg ccaaagctcg gagggcatcc ccatcgcatc ccctaacgcc tgcccataaa
     5701 gagcaccgag aatacgttct gttttcattt aacttcccct tgtgtcaacg caatatcgcg
     5761 atcatcgacc ttgatagccg taatttcgtt gtcggattcg cggaaaaaaa tcatgaacag
     5821 cacggcgata atggcaatca tcaccgcgcc gaaagtccac atcccggacc agttgaaagt
     5881 cagtccgttt accggttcct gataagcgaa catcttttcc atcatcacac cgccaagacg
     5941 atagccgagc aaactgccga agccctggca gcagagcgtg atcagcccct gcgcagcggt
     6001 acgcatatgc acgggggctt ttttatcgac atagatgtaa gcggtaacgt agtaaaaatc
     6061 gtaacttacg ccgtgaagca aaataccgag gaacagtaac gcgtaggtga aatattcatc
     6121 cgtactaccg taaataaaga agccatagcg gatcgcagcg gtgaccagac caagcaataa
     6181 tacctttttg ataccaaagc gtttagtgaa aaacggcaat gccagcataa agaagatttc
     6241 agagaactgg ccgagcgtca tccagccggt ggcgtttttc atgccaactt cggtcagata
     6301 accgttggca aagatgtaat agaacgccag tggcatcgca aacaggaatg aacagaagaa
     6361 aaagacgagg aagtttttat cgcgcagcag gatcagcgca tccaggccga gcatgacttt
     6421 aatatccatt ttgccggtgc tttttggtgg cgtgtcgggc aggaaaaacg caaacacacc
     6481 gagcagagca gaacttccgg cggtaatcag cagcgggatg ttagtcggtg agatatcggc
     6541 ataccccagt atttgcggca agaaaccaca tgccagaccg gaggcaatcc agccgatagt
     6601 gcccatcaca cgaatgcgcg ggaaatcacg ctccacatcc ggcacgttgg caaaagcgat
     6661 gctgttagtc agcgcaatgg tcggcatata ggttagagag taagccagca gtaacggaaa
     6721 gaacccggca aaagtggtct gttgcgcagc gaaatacatc agcagcgcgc ctgcgaacat
     6781 caataccgcc agcacttttt gcgccgagaa aaagcggtca gtgatggagc caaccagaat
     6841 tggcgacagg atcgccgcaa tggcggtaca ggcatacgac cagccaattt ctccggcact
     6901 aaaaccgctt ttacttaacc acaaccacaa tggcacaaac cacgcgcccc agataaacca
     6961 ttcaacaaac atcatgaacg acagctttgc tgttgttttc atcttttaat tccttcatga
     7021 caggtaatgt aaggtacgcg atgacaatac cacttaacaa taccttttaa atacctttgc
     7081 ggtgaaaact tgaccgcgct aacatttttt ctgatgaatc gagccaacag aaaacgctga
     7141 aaaaacatcc aaaagatgga aaaactcgcc cgtaacgacc aggattatga ttgcccgcaa
     7201 aaggcgggcc aggacaatcc atagccgata tccaatcgga atttacggga gcatagtaat
     7261 gacagatatt gcgcagttgc ttggcaaaga cgccgacaac cttttacagc accgttgtat
     7321 gacaattcct tctgaccagc tttatctccc cggacatgac tacgtagacc gcgtaatgat
     7381 tgacaataat cgcccgccag cggtgttacg taatatgcag acgttgtaca acaccgggcg
     7441 tctggctggc acaggatatc tttctattct gccggttgac cagggcgttg agcactctgc
     7501 cggagcttca tttgctgcta acccgctcta ctttgacccg aaaaacattg ttgaactggc
     7561 gatcgaagcg ggctgtaact gtgtggcgtc aacttacggc gtgttggcgt cggtatcgcg
     7621 gcgctatgcg catcgcattc catttctcgt caaacttaat cacaacgaga cgctaagtta
     7681 cccgaatacc tacgatcaaa cgctgtatgc cagcgtggag caggccttca acatgggcgc
     7741 ggtggcggtt ggtacgacta tctattttgg ctcggaagag tcacgtcgcc agattgaaga
     7801 aatttctgcg gcctttgaac gcgcgcacga gctgggcatg gtgaccgtgc tgtgggccta
     7861 tttgcgaaac tccgccttta agaaagatgg cgttgattac catgtttccg ccgacctgac
     7921 cggtcaggca aaccatctgg cggcaaccat cggtgcagat atcgtcaaac aaaaaatggc
     7981 ggaaaataac ggcggctata aagcaattaa ttacggttac accgacgatc gtgtttatag
     8041 caaattgacc agcgaaaacc cgattgatct ggtgcgttat cagttagcta actgctatat
     8101 gggccgggct gggttgataa actccggcgg tgctgcgggc ggtgaaactg acctcagtga
     8161 tgcagtgcgt actgcggtta tcaacaaacg cgctggcgga atggggctga ttcttggacg
     8221 taaagcgttc aagaaatcga tggctgacgg cgtgaaactg attaacgccg tgcaggacgt
     8281 ttatctcgat agcaaaatta ctattgcctg atacgcctca tacgaacagc accaccccgt
     8341 aggacggata aggcgtttac actgcatccg gcaacctatg cctgatgcga cgcggaagcg
     8401 tcttatcatg cctacatagc actgccacgt atgtcggaaa aggcgtttac accgcatccg
     8461 gcataaaaac acgcgcactt tgctacggct tccctatcgg gaggccgttt ttttgccttt
     8521 cactcctcga acagttttca tattgtcgtt ttg
//
LOCUS       DAZRUF010000175         8455 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZRUF010000175 DAZRUF010000000
VERSION     DAZRUF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.175"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(88..294)
                     /locus_tag="ACHXOL_003181"
     CDS             complement(88..294)
                     /locus_tag="ACHXOL_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR6810096.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            607..696
                     /gene="kdpF"
                     /locus_tag="ACHXOL_003182"
     CDS             607..696
                     /gene="kdpF"
                     /locus_tag="ACHXOL_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR6810097.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            696..2369
                     /gene="kdpA"
                     /locus_tag="ACHXOL_003183"
     CDS             696..2369
                     /gene="kdpA"
                     /locus_tag="ACHXOL_003183"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR6810098.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLIALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2392..4440
                     /gene="kdpB"
                     /locus_tag="ACHXOL_003184"
     CDS             2392..4440
                     /gene="kdpB"
                     /locus_tag="ACHXOL_003184"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR6810099.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4449..5021
                     /gene="kdpC"
                     /locus_tag="ACHXOL_003185"
     CDS             4449..5021
                     /gene="kdpC"
                     /locus_tag="ACHXOL_003185"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR6810100.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5014..7698
                     /gene="kdpD"
                     /locus_tag="ACHXOL_003186"
     CDS             5014..7698
                     /gene="kdpD"
                     /locus_tag="ACHXOL_003186"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR6810101.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHIDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7695..8372
                     /gene="kdpE"
                     /locus_tag="ACHXOL_003187"
     CDS             7695..8372
                     /gene="kdpE"
                     /locus_tag="ACHXOL_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR6810102.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1763 a         2202 c         2449 g         2041 t
ORIGIN      
        1 gtctaaatat tcctctgaac atcttcctga tacaaaaaac cgccagcgtt tccgctggcg
       61 gttttcaatg tattcagaac tcagcaatca gtaaaaatca ccggttgcct tttcggcctg
      121 atccatccac accggtttat cgctggtttt cgaccagacg cggtgcaggt agctgtaaaa
      181 gcgggcgcgg tctttccaga aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc
      241 atatgtacgg cgtaaaaaga taagccatgc aggatattct ctgtagagtt ccatactttt
      301 ctcccccttt tgtatctacc cggtgaatag caccggaaaa atgaatttgt ttatctgatg
      361 aaaatagtac cgccttttgt gtaattttac tactcatccg accacttatt tttgcttatt
      421 gatggtttat ttacattcac tctgtaatta agttacacaa aagttaaatt aatactaaac
      481 attagttaaa tcatggcttt tgccattttt atactttttt tacaccccgc ccgcagattt
      541 ttgcgaaatc tttgcagcca gaattctact cttccggtat cacttttagg ccactggagg
      601 tgcactgtga gtgcaggcgt gatagccggc gtgttgctgg tgtttttatt actgggttat
      661 ttggtttatg ccctgatcaa tgcggaggcg ttctgatggc tgcgcaaggg ttcttactga
      721 ttgccacgtt tttactggta ttaatggtgc tggcgcgtcc tttaggcagc gggctggcgc
      781 ggctgattaa tgacattcct cttccaggta cagcgggcgt tgaacgtata ctctttcgtt
      841 tacctggcgt ctctgaccat gagatgaact ggaagcaata tctttgtgcc attctcggcc
      901 tgaacatgct ggggctggcg gtgctgtttt ttatgttgct cggtcagcac tatctgccgc
      961 ttaatccaca gcagttgccg gggctgtcgt gggatctggc gctgaatacc gccgtcagct
     1021 ttgtcacgaa taccaactgg caatcttata gcggtgaaac cacgttgagc tatttcagcc
     1081 agatggcagg cttaacggta caaaactttc tttctgccgc cagcgggatt gcggtgattt
     1141 ttgccttcat ccgtgcgttt actcgccaga gcatgagcac actcgggaat gcctgggtcg
     1201 atctgctacg catcacgtta tgggtgctag tccctgtggc gttgttgatc gcactgtttt
     1261 ttattcaaca aggtgcgctg caaaactttc tgccctatca ggcagtgaat accgttgaag
     1321 gagcgcaaca gctgttaccc atgggaccag tagcttctca ggaagcgatc aagatgctcg
     1381 gtactaacgg cggtggcttc tttaatgcca actcgtcgca tccgtttgaa aacccaaccg
     1441 cactgaccaa cttcgtgcag atgctggcga tcttcttgat cccaacagcg ctgtgctttg
     1501 cctttggtga agtgacgggc gatcgccgcc aggggcgcat gttgctgtgg gcgatgtcag
     1561 tgatttttgt catctgcgta ggcgtggtta tgtgggcaga agttcagggt aatcctcatc
     1621 tgctggcgct cggcgcagac agcagcatca atatggaagg taaagagagc cgtttcggcg
     1681 tgctggtcag tagcctgttt gcggtcgtga ctacggcggc gtcctgtggc gcggtgattg
     1741 cgatgcatga ttcgtttacc gctctcggcg gcatggtgcc gatgtggcta atgcaaattg
     1801 gtgaagtggt gttcggcggt gtcggttctg gtctttacgg catgatgctg tttgtcctgc
     1861 tggcggtgtt tattgccggg ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa
     1921 tcgacgtacg cgagatgaaa ctgattgcac tggcgattct ggttactcca acactggtac
     1981 tgatgggcgc ggcgttggcg atgatgaccg acgccggacg tagcgccatg cttaaccctg
     2041 gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcgtc cgccgctaac aacaacggca
     2101 gcgcctttgc cggattaagc gccaactctc cgttctggaa ctgtttactg gcgttctgca
     2161 tgtttgtcgg tcgcttcggg gtgattatcc cggtgatggc aattgccggt tcgctggtga
     2221 gtaaaaagag ccaacccgcc agctccggca cgctgccaac gcacggcccg ctgtttgttg
     2281 gcctgttaat cggcaccgtg ttgctggttg gcgcactgac ctttatccct gccctggcgc
     2341 ttggtccggt ggcggaatat ctctcctgat gatattgagc gagcactgaa tatgagtcgt
     2401 aaacaactgg cgctattcga accaacactt gtcgttcagg cgctgaaaga agcggtgaaa
     2461 aaattaaacc cgcaggcgca atggcgcaat ccggtgatgt ttatcgtctg gatcggcagt
     2521 ctgctgacca cctgtattag catcgcgatg gcaagcggtg cgatgcctgg caatgcgctg
     2581 tttagcgcgg caattagcgg ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag
     2641 gcgctggcag aaggccgcag taaagcgcag gccaacagtc ttaaaggggt gaaaaaaact
     2701 gcctttgccc gcaagctgcg tgagccgaaa tatggcgctg cggcggacaa agttcctgcc
     2761 gatcaactgc gtaaaggtga catcgtgctg gtagaagctg gcgatattat cccctgcgat
     2821 ggtgaagtca ttgaaggcgg cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggca
     2881 ccggtgatcc gtgaatccgg cggcgatttt gcctccgtca ccggcggcac gcgtattctt
     2941 tctgactggc tggtgattga gtgtagcgtt aaccccggtg agacatttct ggatcggatg
     3001 atcgcgatgg tggaaggcgc acagcgacgc aaaacaccga acgagattgc cctgactatc
     3061 ctgctgattg ctctgaccat cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc
     3121 gcgtggggcg gtaatgccgt gagtgtaacg gtactggtgg cgctgctggt ctgtctgatc
     3181 ccaaccacta ttggcggcct gttgtcagcg atcggcgtcg ccgggatgag tcggatgctg
     3241 ggcgcgaatg tgattgccac cagcggacgt gcagttgaag cggcaggtga cgttgacgtt
     3301 ctgctactgg ataaaaccgg caccatcaca ctcggtaacc gtcaggcgtc ggagtttatc
     3361 cccgcgcagg gcgtggatga aaaaacgctg gctgacgccg cacaactggc ttcgctggct
     3421 gatgaaacgc cggaaggtcg cagtattgtg atcctcgcca agcaacgttt taacctgcgc
     3481 gagcgcgatg tgcagtcgct ccatgccacc tttgtaccgt ttactgcgca aagccggatg
     3541 agcgggatca acatcgacaa ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgccgc
     3601 catattgagg ctaacggcgg tcacttccct gccgatgttg atcaaaaagt cgatcaggtt
     3661 gcgcgtcagg gagccacgcc gctggtggtg gtggaaggtt ctcgtgtgct gggcgttatt
     3721 gcgctgaaag atatcgtcaa aggcggtatt aaagagcgct tcgcccagct gcgcaaaatg
     3781 ggcattaaaa cggtgatgat taccggcgat aaccgtctga ctgccgccgc gatagctgcg
     3841 gaagcgggtg tcgatgattt tctcgccgaa gcaacaccgg aggccaagct ggcattgatc
     3901 cgtcagtatc aggcggaagg tcgtttggta gcgatgaccg gcgacggcac caacgatgct
     3961 ccggcgctgg cgcaggcaga tgtcgcggtg gcgatgaact ccggcaccca ggcggcgaaa
     4021 gaggcgggca atatggtgga tctcgactct aacccgacca agttgatcga ggtggtgcac
     4081 attggcaaac agatgctgat gacccgtggc tcgctgacca ccttcagcat tgccaacgat
     4141 gtggcgaaat acttcgccat tattccggcg gcgttcgcgg caacatatcc gcagttaaat
     4201 gcgctgaaca tcatgcgcct gcattcgccc gactccgcca ttctcagtgc ggtcattttc
     4261 aacgccttga ttatcgtctt tttgattcct ctggcgttaa aaggcgtgag ttataaaccg
     4321 cttaccgctt ctgccatgtt gcgccgtaac ttatggattt acggtctggg tgggctgctg
     4381 gtgccgttta ttggtatcaa agtcattgat ttactgctga ccgtttgcgg tctggtgtga
     4441 ggtttaccat gagtggatta cgtccggcat tatcgacatt tctctttctg ttattgatta
     4501 ctggcggcgt ttacccgctg ctgaccaccg cactggggca atggtggttt ccctggcagg
     4561 ccaacggttc gttgatccgt gaaggtgata cggtgcgtgg ctcggcatta atcgggcaga
     4621 attttaccgg caacggctat tttcatggtc gcccgtcagc aacggctgaa atgccctata
     4681 atccacaggc ttctggcggg agcaatctgg cggtcagtaa ccctgagctg gataaactaa
     4741 tagccgcacg cgttgctgca ttacgggccg ctaacccgga tgccagcgcg agcattccgg
     4801 ttgaactggt gacggcatcg gcaagcgggc tggacaataa tatcaccccg caagcggcgg
     4861 cctggcagat cccacgcatc gcgaaagcgc gtaatctcag cgttgaacag ctcacgcagc
     4921 tgatcgcaaa atacagccaa caaccgctgg tgaaatatat cggccagccg gtcgtcaaca
     4981 ttgtcgaact caatctggcg ctggataagc ttgatgaata acgaaccctt acgtcccgac
     5041 cccgatcgtc tgctggaaca aactgccgcg ccgcatcggg ggaagctgaa agttttcttc
     5101 ggtgcctgtg caggcgtcgg gaagacctgg gcgatgctgg cagaagccca gcgactgcgg
     5161 gcgcaagggc tggatattgt ggttggcgtg gtagaaaccc acgggcgaaa agataccgcc
     5221 gcaatgctgg aagggctggc tgttctgccg ccaaaacgcc tggcgtaccg tgggcggcac
     5281 atctgcgagt ttgacctcga tgccgccctc gcccgccgtc cggcgctgat cttaatggac
     5341 gaactggcgc acagtaatgc gccaggttct cgtcacccta aacgctggca ggacatcgaa
     5401 gagctgctgg aagcaggcat tgatgttttc actaccgtca acgttcagca tctggaaagt
     5461 ctgaatgatg tggtcagcgg cgtcaccgga attcaggtac gggaaaccgt gcccgatcct
     5521 tttttcgatg ccgccgacga cgtggttctg gtggacttgc ccccggacga tctgcgccag
     5581 cggctgaaag aaggcaaagt ctatattgcc gggcaggcgg agcgcgccat tgaacatttt
     5641 ttccgcaaag gcaatctgat cgccctgcgc gaactggcac tgcgccgtac tgccgatcgc
     5701 gttgatgagc aaatgcgcgc ctggcggggg catcctagcg aagagaaagt ctggcacacg
     5761 cgcgacgcga tccttttatg catcggccat aacaccggta gtgagaagct ggtccgcgcg
     5821 gcggcgcggc tggcgtcacg gctgggtagc gtctggcacg cggtgtatgt tgaaacccct
     5881 gccctgcatc gcttaccgga gaaaaaacgt cgtgcaattc tcagcgcctt acgtctggcg
     5941 caggaactgg gcgcggagac ggcaacactt tctgatccag cggaagagaa agcggtagtg
     6001 cgttatgccc gtgaacataa tctcggcaag attattctcg gtcgcccggc ctcgcgccgc
     6061 tggtggcgtc gggaaacgtt tgctgaccga ctggcgcgca tcgcccccga tctcgatcag
     6121 gtgctggtcg cgcttgatga accacccgcc cgcacgatta acaacgcgcc ggatagccgc
     6181 tcttttaaag acaagtggcg ggtacagatt cagggatgcg tggttgccgc cgcgttatgc
     6241 gccgttatca ccttaattgc catgcagtgg ctgatggcgt ttgatgccgc caacctggtg
     6301 atgctgtatc tgcttggcgt ggtggtggtg gcgctatttt atggacgctg gccttcggtg
     6361 gttgccaccg tcattaatgt ggtgagtttc gatctctttt ttatcgcccc acgcggcacg
     6421 ctcgccgtct ctgatgtgca atatctgctg accttcgcgg tgatgttaac cgtcgggctg
     6481 gtgatcggga accttactgc tggcgtgcgt tatcaggcgc gggtagcccg ttaccgcgag
     6541 caacgcacac ggcacttata tgaaatgtcg aaagctctgg cggtgggccg cagtccgcag
     6601 gacatcgccg ccaccagcga acaatttatt gcctccacgt ttcatgcccg cagtcaggtg
     6661 ttgttgcccg atgacaacgg taaattgcag ccgttaacac atccgcaagg aatgacgcca
     6721 tgggacgatg ccatcgcgca gtggagtttt gataaaggcc tgcctgcggg cgcgggcacc
     6781 gacacgttac ccggtgtacc gtaccagatt ttgccgctaa aaagcggcga gaaaacctac
     6841 gggctggtgg tggtggagcc ggggaatctg cgccagttga tgatcccgga acagcagcgc
     6901 ctgctggaga cgtttacgct gttagtcgcc aatgcccttg agcggctgac gctaaccgcc
     6961 agcgaagaac aggcgcggat ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggca
     7021 gcgctttcgc atgatttacg cacgccgctt acggtgctgt ttggtcaggc agaaatctta
     7081 acgctcgatc tggcaagcga aggatcaccc cacgcccgcc aggccagcga gattcgtcag
     7141 catgtgctga acactacccg actggtgaat aatctactgg atatggcgcg aattcagtcc
     7201 ggcggcttta atttgaagaa agagtggtta acgctggaag aagtagtcgg cagcgcgctg
     7261 caaatgctgg aaccgggttt atcgtcgccc atcaatcttt ctctgccaga accgctgacc
     7321 ttaatccaca ttgacgggcc actctttgaa cgggtgctga ttaatctgct ggagaacgcg
     7381 gtgaaatatg cgggtgcgca ggccgaaatt ggtatcgatg cccactttga gggcgaaaat
     7441 ctacaactgg atgtctggga taacggcccc ggtcttccgc caggccagga gcagacgata
     7501 tttgataagt ttgctcgcgg gaataaagag tcggcggtgc cgggtgtcgg gcttgggttg
     7561 gcaatttgtc gggcgattgt ggatgtacac ggcggcacca ttaccgcgtt caaccgaccg
     7621 gaaggtggtg cctgttttcg tgttacactt ccccagcaaa ccgctcctga acttgaagac
     7681 tttcatgagg atatgtgaca aacgttctga ttgttgaaga tgaacaggct attcgtcgct
     7741 ttctgcgcac ggcgctggag ggcgacggga tgcgcgtctt tgaggccgaa actctgcaac
     7801 gcggcttgct ggaagcggca acccgtaagc cagatttgat tattctcgat ctcggcctgc
     7861 ccgatggtga tgggattgag tttatccgcg acctgcgcca gtggagcccg gtgccggtga
     7921 ttgtgctttc cgcacgcagc gaagagagcg acaagatcgc cgcgctggat gccggagcgg
     7981 atgattatct gagtaagccg tttggcattg gcgaattgca ggcccgtctg cgcgtcgctt
     8041 tacgccgcca ctccgccacc gccgcgcccg atccgctggt gaaattttcc gatgttaccg
     8101 tcgatttagc cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg
     8161 agttccgcct gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc
     8221 tccttaacca ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata
     8281 tgggacatct gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg
     8341 aaaccggtat tggatatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata
     8401 gtgattaatt tattattaaa cctgcgccga atattattat ttggcaggtt taatt
//
LOCUS       DAZRUF010000176         8377 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZRUF010000176 DAZRUF010000000
VERSION     DAZRUF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.176"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            159..1136
                     /gene="glaH"
                     /locus_tag="ACHXOL_003188"
     CDS             159..1136
                     /gene="glaH"
                     /locus_tag="ACHXOL_003188"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR6810103.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1156..2424
                     /gene="lhgO"
                     /locus_tag="ACHXOL_003189"
     CDS             1156..2424
                     /gene="lhgO"
                     /locus_tag="ACHXOL_003189"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR6810104.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2447..3895
                     /gene="gabD"
                     /locus_tag="ACHXOL_003190"
     CDS             2447..3895
                     /gene="gabD"
                     /locus_tag="ACHXOL_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR6810105.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3909..5189
                     /gene="gabT"
                     /locus_tag="ACHXOL_003191"
     CDS             3909..5189
                     /gene="gabT"
                     /locus_tag="ACHXOL_003191"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR6810106.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5500..6900
                     /gene="gabP"
                     /locus_tag="ACHXOL_003192"
     CDS             5500..6900
                     /gene="gabP"
                     /locus_tag="ACHXOL_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR6810107.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6921..7583
                     /gene="csiR"
                     /locus_tag="ACHXOL_003193"
     CDS             6921..7583
                     /gene="csiR"
                     /locus_tag="ACHXOL_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR6810108.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7584..8033)
                     /gene="kbp"
                     /locus_tag="ACHXOL_003194"
     CDS             complement(7584..8033)
                     /gene="kbp"
                     /locus_tag="ACHXOL_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR6810109.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8117..8275)
                     /locus_tag="ACHXOL_003195"
     CDS             complement(8117..8275)
                     /locus_tag="ACHXOL_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR6810110.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1889 a         2191 c         2352 g         1945 t
ORIGIN      
        1 ctctacgtta aatgttacta atttgttgct tttgatcaca ataagaaaac aatatgtcgc
       61 ttttgtgcgc atttttcaga aatgtagata tttttagatt atggctacga aatgagcatc
      121 gccatgtcac cctacatctc ataagaggat cgcttctgat gaatgcactg accgccgtac
      181 aaaataacgc tgtcgattca ggccaggact atagcggatt cactctcatc ccgtcggcgc
      241 aatccccgcg tctgctggaa ctcaccttca ccgaacagac gaccaaacag tttctcgagc
      301 aggttgccga atggcccgta caggcgctgg agtacaaatc gtttctgcgt tttcgggtag
      361 gcaaaattct tgacgatctg tgtgcgaatc agctgcaacc attgctgttg aagaccctgc
      421 taaaccgtgc tgaaggtgcg ctgttgatca atgcggtggg tatcgatgat gtggcgcagg
      481 cagatgagat ggtgaagctg gcaacggcgg tagcgcatct gattggtcgc tcaaatttcg
      541 atgcgatgag cggtcagtat tacgcgcgtt tcgtggtgaa aaatgtcgat aactcagaca
      601 gctatctgcg tcagccgcac cgcgtgatgg aactgcacaa cgacggcacg tatgtcgaag
      661 agatcaccga ttacgtgctg atgatgaaaa tcgatgagca aaacatgcag ggtggaaatt
      721 cgttgctgct gcatctcgat gactgggaac atctggacca ctatttccgc catccactgg
      781 cgcgtcgccc gatgcgcttt gccgcgccgc cgagcaaaaa cgtcagcaaa gatgttttcc
      841 atccagtgtt cgacgttgat caacagggtc gcccggtgat gcgctatatc gaccagttcg
      901 tccagccaaa agattttgaa gaaggcgtgt ggttgagcga gctttccgat gccattgaaa
      961 ccagcaaagg cattctttct gtacccgttc ccgttggcaa attcctgttg ataaacaacc
     1021 tgttctggct gcacggtcgc gaccgcttta ctccgcaccc ggatctgcgc cgtgaactga
     1081 tgcgtcagcg tggctatttc gcttacgcca ctcaccacta ccagacgcat cagtaagcgc
     1141 aaaggaattg agcggatgta tgattttgtg attattggcg gcggcatcat cggcatgtcg
     1201 accgccatgc aactgattga tgtttatccg gatgcccgca ttgcgttgct ggaaaaagag
     1261 tccggcccgg cctgtcacca gacgggccac aacagcggcg tgatccatgc cggggtctat
     1321 tacacgcccg gcagcctgaa ggcgcagttt tgcctggcgg ggaaccgcgc caccaaagcc
     1381 ttttgcgatc aaaacggcat tcgctatgac aactgcggca agatgctggt cgccacgtct
     1441 cagctcgaaa tggaacggat gcgcgcctta tgggaacgca cagcggcgaa cggtatcgag
     1501 cgcgagtgga taaacgccgt ggaactgcgc gagcgcgaac cgaatatcac cgggcttggt
     1561 ggcatttttg tgccgtccag cggcattgtc agctaccgcg aagtcacagc ggcaatggca
     1621 aaaatcttcc aggccagagg cggcgagatt atttataacg ccgaagtcag cgccctcagt
     1681 gagcataaaa acggcgtggt gatacgcacc cgtcagggcg gtgaatatga agcatcgacg
     1741 ctgattagct gttccgggct gatggctgac cggctggtga aaatgctcgg cctcgaaccg
     1801 ggctttatca tctgcccgtt ccgtggtgag tatttccgcc ttgcgccgga gcataaccag
     1861 attgttaacc acctgattta ccccattccc gaccccgcga tgccattttt gggcgtgcat
     1921 ctcacccgca tgatcgacgg cagcgtgacc gtcgggccaa acgcggtgct ggctttcaaa
     1981 cgcgaaggct atcgcaagcg cgacttctca tttagtgaca cgctggagat tttgagctcg
     2041 tcggggattc gccgggtgct gcaaaaccat ctacgctcag gactgggcga gatgaaaaac
     2101 tcgctgtgca aaagcggcta cctgcggctg gtgcaaaagt attgtccccg gctttcgtta
     2161 agcgatctcc agccctggcc cgccggtgtg cgggcgcagg cagtatcgcc ggacggcaag
     2221 ctgattgacg attttctgtt tgtcaccacc ccacgcacga tccacacctg caatgcgccc
     2281 tccccggcag cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg
     2341 ctgttggcaa gccagagtaa ccccggacgc acgctgcgag cggcacgtag tgtggatgcc
     2401 ttacacgccg catttaatca ataacctttg aaaacaggac gtaacaatga aacttaacga
     2461 cagtaactta ttccgccagc aggcgttgat taacggggaa tggctggacg ccaacaatgg
     2521 cgggatcatc gacgtcacca atccggcgaa cggcgacaag ctgggtagcg tgccgaaaat
     2581 gggcgctgat gaaacccgcg ccgctatcga cgccgccaac cgcgccctgc ccgcctggcg
     2641 tgcgctcacc gccaaagaac gcgccaacat tctgcgcaac tggttcaatt tgatgatgga
     2701 gcatcaggac gatttagcgc gcctgatgac cctcgaacag ggcaaaccac tggccgaagc
     2761 gaaaggcgaa atcagctacg ctgcctcttt tattgagtgg tttgccgaag aaggcaaacg
     2821 tatttatggc gataccattc ccggtcatca ggccgataag cgcctgattg ttatcaagca
     2881 gccgattggc gttaccgcag ctatcacgcc gtggaacttc ccggcggcga tgattacccg
     2941 taaagccggt ccggcgctgg cggcaggctg caccatggtg ctgaagcccg ccagtcagac
     3001 accgttctct gcgctggcgc tggcggagct ggcgattcgc gcgggcattc cggctggcgt
     3061 gtttaacgta gtcaccggtt cggcaggcgc ggtcggtaat gaactgacca gcaacccgct
     3121 ggtacgcaaa ctgtcgttta ccggttcgac cgaaattggc cgccagttaa tggaacagtg
     3181 cgcgaaagac atcaaaaaag tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt
     3241 tgacgatgcc gatctcgata aagccgtgga aggcgcgctg gcctcgaaat tccgcaacgc
     3301 cgggcaaacc tgcgtctgcg ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt
     3361 tgccgaaaaa ttgcagcagg cggtgagcaa actgcacatc ggcgacgggc tggataaagg
     3421 cgtcaccatc gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc
     3481 cgatgcgctg gagaaaggtg cgcgtgtggt ttgcggcggt aaagcggatg aacgcggcgg
     3541 caacttcttc cagccgacca ttctggtgga cgtgccggcc aacgccaaag tgtcgaaaga
     3601 agagacgttc ggccccctcg ccccgctgtt ccgctttaaa gatgaagccg atgtgattgc
     3661 gcaagccaat gacaccgagt ttggccttgc cgcctatttc tacgcccgtg atttaagccg
     3721 cgtcttccgc gtgggcgaag cgctggagta cggcatcgtc ggcatcaata ccggcattat
     3781 ttccaatgaa gtggccccgt tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc
     3841 gaagtatggc atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga
     3901 gaatgcgaat gaacagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg
     3961 gcgttgggca aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg
     4021 ttgaaggccg tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcacc
     4081 tgcatccgaa ggtggtggcc gcagtggaag cgcagttgaa aaaactgtcg cacacctgct
     4141 tccaggtgct ggcttacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc
     4201 cgggcgattt cgccaagaaa acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg
     4261 cggtaaaaat cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgcgt
     4321 atcacgggcg cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg
     4381 gcatggggct gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca
     4441 taagcgagga tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg
     4501 aagatatcgc cgccatcgtg attgagccgg ttcagggcga aggcggtttc tacgccgcgt
     4561 cgccagcctt tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg
     4621 ccgatgaagt gcagagcggc gcggggcgta ccggcacgct gtttgcgatg gagcagatgg
     4681 gcgtggcgcc ggatcttacc acctttgcga aatcgatcgc aggcggtttc ccgctggcgg
     4741 gcgtcaccgg gcgcgcggaa gtgatggacg ctgttgcacc gggcggtctg ggcggcacct
     4801 atgccggtaa cccgattgcc tgcgtggctg cgctggaagt gttgaaggtg ttcgagcatg
     4861 aaaatctgct gcaaaaagcc aacgatctgg ggcagaagct aaaagacgga ttgctggcga
     4921 tagccgaaaa acacccggag atcggcgacg tacgcgggct gggagcgatg attgccatcg
     4981 agctgtttga agatggcgat cacagcaaac cggacgcaaa actcaccgcc gagatcgtgg
     5041 cacgcgcccg cgataagggc ctgattcttc tctcctgcgg cccgtattac aacgtgctgc
     5101 gcatccttgt accgctcacc attgaagacg ctcagatccg tcagggtctg gagatcatca
     5161 gccagtgttt tgctgaggcg aagcagtagc tatgtccgtt tgtaagagtt aaccgttgct
     5221 aagggaatga taacgatgca gtaaaaaata ataacgatga ggcgaacgac aaagttaaaa
     5281 acgactgcgg acagtcccct cgcccctctg gagagagggt tagggtgagg ggtaaaacac
     5341 gcggctaaac cctcccctgg ccctctccct aagggagagg gagaaaaccc tcccgctttc
     5401 gtttttaaga ttttgttgac cgtctcaatt aaacgcgtga cccggcgcgg gaaatgtcgg
     5461 ggcgctctcc caagtgacac actttcgaga ggattcagga tggggcaatc atcgcaacca
     5521 catgagttag gcggcgggct gaaatcacgc cacgtcacca tgttgtctat tgccggtgtt
     5581 atcggcgcaa gtctgtttgt cggttccagc gtcgccatcg ccgaagcggg cccggcggta
     5641 ttactggcct atctgttcgc cgggctactg gtggttatga ttatgcggat gttggcggaa
     5701 atggcggttg ccacgcccga taccggttcg ttttccacct atgccgataa agccattggt
     5761 cgctgggcgg gctataccat cggctggttg tactggtggt tttgggtact ggttatcccg
     5821 ctggaagcca atatcgccgc tatgatcctg cactcgtggg ttccaggcat tcccatctgg
     5881 ttattttccc tcgtcattac ccttgcctta actggcagta atttattaag cgttaaaaac
     5941 tacggcgaat ttgagttctg gctggcgctg tgcaaagtca tcgctatcct ggcctttatt
     6001 ttccttggtg cagtcgcaat tagcggtttt tacccttatg ccgaagtgag cgggatctca
     6061 agattgtggg atagcggcgg ctttatgccc aacggtttcg gtgcggtatt aagcgcgatg
     6121 ttgatcacca tgttctcgtt tatgggcgca gaaattgtca ccattgccgc cgcggaatcc
     6181 gacacgccgg aaaaacatat tgtccgcgcc accaactcgg ttatctggcg tatttctatc
     6241 ttctatttgt gctctatttt tgtcgtcgtg gcattaatac cgtggaatat gccggggctg
     6301 aaagccgttg gatcttatcg ctcggttctg gaattgctca atattcccca tgcgaaatta
     6361 atcatggact gtgtgatatt actttccgta accagttgcc tgaactcggc cctgtatacc
     6421 gcctcaagga tgctctactc cttaagccgt cgcggtgatg ctcccgcggt aatgggtaaa
     6481 atcaaccgca gtaaaacccc gtacgtggcg gtgttactct ccaccggcgc ggcattctta
     6541 acggtggtgg tgaactatta cgcgcctgcg aaggtattta aatttctgat cgacagctcc
     6601 ggcgctatcg ccctgctggt ttatttagtc atcgccgttt cacagttgcg gatgcgcaaa
     6661 attctgcgag cggaaggaag cgaaattcgc ttgcgaatgt ggctttatcc ctggctcacc
     6721 tggctggtca tcggctttat tacctttgtg ttggtagtga tgctattccg tccggcgcaa
     6781 cagttagaag tgatctccac cggcttatta gcgataggga ttatctgtac cgtgccaatt
     6841 atggctcgct ggaaaaagct ggtattgtgg caaaaaacac ccgttcataa tacgcgctga
     6901 taatgatcag gagtcacacc atgaccatta cgtctctgga tggctatcgc tggctgaaga
     6961 acgatattat tcgcggtaat tttcaaccgg atgaaaaatt acggatgagt ttgctgacat
     7021 cgcgttatgc acttggcgtt gggccgttgc gggaagcact ttctcaactg gtggcggaac
     7081 ggctggtcac ggtggtgaat caaaaagggt atcgggtggc gtctatgtca gagcaggagc
     7141 tgctcgatat tttcgatgcc cgcgccaata tggaagcgat gttagtgagt ctggcgattg
     7201 cccgcggtgg cgatgagtgg gaggcagacg ttctcgcaaa agcgcatctg ctcagtaagc
     7261 tggaagcctg tgacgccagc gagaaaatgc ttgatgagtg ggatctgcgt catcaggcgt
     7321 ttcatacagc aattgtggcg ggctgtggtt ctcactattt gctgcaaatg cgtgaacggt
     7381 tgtttgatct ggcggcgcgc tatcgattta tctggctgcg gcgaacggtg ctttcggtag
     7441 aaatgctgga ggataaacac gatcagcacc agaccctgac tgcggcggta ctggcgcgag
     7501 ataccgcgcg cgccagtgag ttaatgcgcc agcatttact gacgccaatt cccattatcc
     7561 agcaggcgat ggctggcaat taactactct tccggaatac gcaacacttg ccccggataa
     7621 attttatccg ggctttttag catcggttta ttcgcttcga agattttatt gtacagatta
     7681 gcgttaccgt agacctgttt ggaaatggca ctcagagtgt cgccagactt aacggtataa
     7741 aactggctgg cagtggctgg tgtcgccgtt ttcacctgat catcaacact ggcaataccg
     7801 gaaatattcc ccaccgcaac aaggattttc tccttcgcct cctgactcag gccgtcacca
     7861 gtgaccgtcg ctttgccgtc ggcaatttga atgttcactt tatcagcatc aggtataccg
     7921 gttttgctca gatgctcctg caccttcttc gcctgatcgt ctttatcgtg ctgacctgta
     7981 accgcgtccc agagtttttc tccggcatct ttcacaaaat tgaacagacc catagctacc
     8041 tcattcgtta acggaaacac tcagaaagtg tagcagagga acgcggcgcg ttaacgccgc
     8101 gggggtgagc acaggtttaa tcgcgggttt gcacccagaa cgcgtgaatc aaaccgggga
     8161 tatacccaag cagtgtcagc agaatattaa taatgaacgc ccaaccgaac cctttaccga
     8221 gcagcacgcc gagcggcggt agaatgatgg tgatgacgat tctccagaaa cccatatgta
     8281 ctccctataa gaaaattact cattgtttaa aaagagattt tatctcttaa gcgtagtaat
     8341 tttagcggag cctgccagtt tcagcttacg cttttac
//
LOCUS       DAZRUF010000177         8376 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZRUF010000177 DAZRUF010000000
VERSION     DAZRUF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.177"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(113..189)
                     /locus_tag="ACHXOL_003196"
     tRNA            complement(113..189)
                     /locus_tag="ACHXOL_003196"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(153..155),aa:Asp,seq:gtc)
     gene            complement(322..1053)
                     /gene="dnaQ"
                     /locus_tag="ACHXOL_003197"
     CDS             complement(322..1053)
                     /gene="dnaQ"
                     /locus_tag="ACHXOL_003197"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR6810111.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1118..1585
                     /gene="rnhA"
                     /locus_tag="ACHXOL_003198"
     CDS             1118..1585
                     /gene="rnhA"
                     /locus_tag="ACHXOL_003198"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR6810112.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1582..2304)
                     /locus_tag="ACHXOL_003199"
     CDS             complement(1582..2304)
                     /locus_tag="ACHXOL_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR6810113.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2338..3093
                     /gene="gloB"
                     /locus_tag="ACHXOL_003200"
     CDS             2338..3093
                     /gene="gloB"
                     /locus_tag="ACHXOL_003200"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR6810114.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3165..4523
                     /gene="mltD"
                     /locus_tag="ACHXOL_003201"
     CDS             3165..4523
                     /gene="mltD"
                     /locus_tag="ACHXOL_003201"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR6810115.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4572..5195)
                     /locus_tag="ACHXOL_003202"
     CDS             complement(4572..5195)
                     /locus_tag="ACHXOL_003202"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR6810116.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5199..5999)
                     /locus_tag="ACHXOL_003203"
     CDS             complement(5199..5999)
                     /locus_tag="ACHXOL_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR6810117.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6240..7154
                     /gene="yafC"
                     /locus_tag="ACHXOL_003204"
     CDS             6240..7154
                     /gene="yafC"
                     /locus_tag="ACHXOL_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR6810118.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7151..7954)
                     /gene="dkgB"
                     /locus_tag="ACHXOL_003205"
     CDS             complement(7151..7954)
                     /gene="dkgB"
                     /locus_tag="ACHXOL_003205"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR6810119.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8117..8193)
                     /locus_tag="ACHXOL_003206"
     tRNA            complement(8117..8193)
                     /locus_tag="ACHXOL_003206"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8157..8159),aa:Asp,seq:gtc)
BASE COUNT         2234 a         2021 c         2025 g         2096 t
ORIGIN      
        1 acatttacag cataacaaaa ccatagaccg aagaaatagc gggatttaaa tgcaaaaaaa
       61 ccgcaaaatt aaaaatcttg cggttctctg aactcatttt catgagtgaa tttggcggaa
      121 cggacgggac tcgaacccgc gcccccctgc gtgacaggca ggtattctaa ccgactgaac
      181 taccgctccg cgttgtgttc cgttgggaac gaggcgaata ttacggattg cctcaccctt
      241 cgtcaacggt ttttctcatc ttttgaatcg tttgctgcaa aaatcgccca agccgctatt
      301 tttagcgcct tttacaggta tttatgcccg ccagaggcag cttccgccct tcttctccac
      361 cagatcaaga cgggcttcat gagctgcaag ctcttcatct gtcgcaaaaa caacgcgtaa
      421 cttacttgcc tgacgtacaa tgcgctgaat tgttgcttca ccttgttgct gctgtgtctc
      481 tccttccatc gcaaaagcca tcgacgtttg accaccggtc atcgccagat aaacttccgc
      541 aaggatctgt gcatcgagta atgccccgtg tagcgttcgt ttactgttat ctatttcgta
      601 gcgggcacat aacgcatcga ggctgttgcg cttaccagga aacattttcc tcgccaccgc
      661 aaggctatcg gtgaccttac agaaggtatt ggtcttcgga atatcgcgct taagcaacga
      721 aaactcgtag tccataaagc cgatatcgaa cgctgcgtta tggatcacca actccgcgcc
      781 gcggatatag tccatgaact catcggctac ttcggcaaac gtgggcttat cgagcaaaaa
      841 ttcatcggca ataccatgta cgccaaaggc ttccggatcc accagccgat cgggtttgag
      901 ataaacatgg aagttattgc ccgtcaggcg acggttcacc acttcaacgg caccaatctc
      961 aatgatcttg tggccttcat agtgcgcacc aatctggttc ataccggtgg tttcggtatc
     1021 gagaacgatc tggcgtgtaa ttgcagtgct catagcggtc atttatgtca gacttatcgt
     1081 tttacagttc gattcaatta caggaagtct accagagatg cttaaacagg tagaaatttt
     1141 caccgatggt tcgtgtctgg gcaatccagg acctgggggt tacggcgcta ttttacgcta
     1201 tcgcggacgc gagaaaacct ttagcgcagg ctacacccgc accaccaaca accgtatgga
     1261 gttgatggcc gctattgtcg cgctggaggc gttaaaagaa cattgcgaag tcattttgag
     1321 taccgacagc cagtatgtcc gccagggtat cacccagtgg atccataact ggaaaaaacg
     1381 tggctggaaa accgcagaca aaaaaccagt aaaaaatgtc gatctctggc aacgtcttga
     1441 tgctgcccta gggcaacatc aaatcaaatg ggaatgggtt aaaggccatg ccggacaccc
     1501 ggaaaacgaa cgctgtgatg aactggctcg tgctgcggca atgaatccca cactggaaga
     1561 tacgggctac caagtcgaag tttaagcttg tggtttacga cattgccggg tggctccaac
     1621 cgcctggcga attcgtggct tgtttttact ctgtttcatc gggtttagcg ttaaaggaat
     1681 agtccgtttc cgggcaacaa taagttgtaa gcaaccaagc gcaggaatat gcgcattcaa
     1741 tagttttcct ccgtgtttgt tccacgggag aacgtggaaa cggctggcgt gcagcacttc
     1801 aaaattcaac aaagagagcc agtccagttg ccgcatcaga gtaaacatcc ggctgttata
     1861 gggcgaggtt tttcgcaata ccggcacaag tttgcgtaat cccattaaac taatgggatt
     1921 gaagccacta atcaccagcc agccatcatc aatcaatacc cgatcggttt cacgcaataa
     1981 acgatgcgga tcggtgcacc acggcaatgt atgtgccagt agacaaacat caacggattt
     2041 atcggcaaaa ggaagatgaa gtggatccgc ctgcacctgg acgggcattc cttgcgcaga
     2101 aacattcact tgatgagaaa ccgcgcacgc ttcgcaattg atttctgcgc ttaaattgcc
     2161 aatcttaagc agatgaaaac catacatttt agtgaaccac gggttgagct ggcgctccag
     2221 cgccttgcga taaagctctc cccagggcaa atcgccccag cgatcaggag ccacgacagt
     2281 ttgagggact cttgccggtt tcatcacaac cttccgtttc acactaagag gtaatctatg
     2341 aatcttaaca gtattcccgc ctttgatgac aattacatct gggttttgaa tgatgaagca
     2401 ggtcgctgcc tgattgtcga tcccggagac gcagagccag tattaaacgc cattgccgcc
     2461 aataactggc aaccggaggc catatttctc acccaccatc atcacgatca cgttggcggc
     2521 gtaaaagaac tggtggaaaa gtttccgcaa attgtggtgt atggtccaca agagacacaa
     2581 gataagggaa caacacaggt agtcaaagat ggcgaaactg ccttcgtttt ggggcatgaa
     2641 tttagtgtaa ttgccacacc gggtcacact ttaggacata tctgttactt cagtaaacct
     2701 tatctatttt gcggcgacac gctgttttct ggtgggtgtg gtaggttgtt tgaagggaca
     2761 gcatcacaaa tgtatcaatc acttaataag ttaagtgcgt tacctgacga tacattggta
     2821 tgttgtgctc atgaatatac gttatcaaat atgaagtttg ctttgagtat tcttccgcac
     2881 gatttgtcca taaatgatta ttatcgtaaa gttaaggagt tacgggcaaa aaatcaaata
     2941 acactacccg taattctgaa aaatgagcgg caaattaatg tttttttaag aacggaagat
     3001 attgatttaa ttaatgtaat taatgaagaa acattattgc aacaacctga agagcgtttt
     3061 gcatggttaa ggtcaaagaa agataggttc tgataaaact ttcttgtcat cggctccgtt
     3121 cgccgttatg atcggtcgtc ttttaagcaa ctattgacac acacatgaag gcaaaagcga
     3181 tattactcgc ctctgtcctg ctcgtgggtt gccagagtac cggcaacgtt caacagcacg
     3241 cacagagcct ttctgcagct ggtcaagggg aagcagcaaa gtttacaagt caggcacgat
     3301 ggatggacga tgggacgtct atcgcgccag atggtgactt gtgggctttc attggcgacg
     3361 agctaaagat gggaattccg gaaaatgacc ggattcgcga acagaaacag aaatatttac
     3421 gcaataagag ctatctccac gatgtaactt tacgggcaga gccgtatatg tactggatag
     3481 cagggcaagt taaaaaacgt aacatgccta tggaactggt actactaccc atagtggaga
     3541 gcgcttttga tcctcacgca acgtctggcg ccaatgccgc aggcatctgg cagatcattc
     3601 cgagcacggg gcgcaattat ggtttgaaac agacccgcaa ttatgacgcg cgtcgcgatg
     3661 ttgttgcttc aacaactgcc gcgctgaata tgatgcagcg tctgaacaaa atgtttgacg
     3721 gcgactggct tctgaccgta gcggcttata acagcggcga aggtcgggtc atgaaggcaa
     3781 ttaaaacgaa caaagcgcgt gggaaatcca cggacttctg gtcgttaccg ttgccgcagg
     3841 aaacgaagca gtacgtgcct aaaatgctgg cattgagtga tattctcaaa aacagcaagc
     3901 gttatggcgt acgtctgcca acgaccgatg aaagccgtgc tctggcgcgt gtgcacctga
     3961 gtagcccggt tgaaatggcg aaggttgcag atatggcggg gatttccgtc agcaagctga
     4021 agacattcaa cgctggcgtg aaaggctcca cgctgggcgc aagtgggccg cagtacgtga
     4081 tggtgccaaa gaagcatgca gatcaactgc gtgaatctct cgcttcaggc gaaattgctg
     4141 ctgtacagtc gacgctggtt gccgacaata cgccgcttaa cagccgtgtt tacaccgtac
     4201 gctctggcga cacgctttca agcatcgctt cacgtctcgg cgtaagcacc aaagatttgc
     4261 agcagtggaa caaactgcgc ggatctaagc tgaagccagg ccaaagtttg acgattggtg
     4321 caggcagtag cgcacagcgg ttggcaaaca acagcgatag cattacgtat cgtgtgcgca
     4381 aaggcgattc gctttcaagc attgctaaac gccacggcgt gaacatcaaa gatgtgatgc
     4441 gctggaacag cgacactgcg aatctgcaac caggcgataa gctgacgttg tttgtgaaaa
     4501 acaacaacat gccagattcc tgacaaacca gataataaaa aaggcaccga ttcccccggt
     4561 gcctttttta tttatgccgc tttatgtgct tctaccatga tgatatcact ggtgaaagag
     4621 ccatcagtct gcaaggaaaa atacgttctc acctctgtcg atgcgctctg ttggtaaatg
     4681 cgaatagcgt ctactaacgc ttctggcgta cgcattctca cgacccatga agaaaattcc
     4741 agcggtaact tatctgtaat taaattatca actatcagat tggcttcatt gattaacgtc
     4801 aaccacgcac cgctggcgta gtttcgtacg tgagaggtat cgcgtaatgc ttctaccgcc
     4861 tgtaaccaga tgtcgcgcac tgggtgaccc ggagacatta catccatcac aatcagccta
     4921 ccgccaggtt tcaatatcct attcacttct cgcagtgctg caccaacatc atgccagtga
     4981 tgggcagaat aacggctgat aacaatatca aatgcgttat cggcaaatgg cagacttgcg
     5041 gcatatccct ggcgggtggt gatatttttc agttgccggg cttcggcagc ttgtgccacg
     5101 acatccagca tgtgggcaga taagtcatac gccaccaccg cgctcacgtt ttgcgcagcg
     5161 ccaaacgttg gtctgccctg cggcagacct gacatacctt atttatcagg cttgccggga
     5221 ctgaattcaa cgagtagcgg attgtgatcg gaagcgcgcg taaccagtac agaagcttca
     5281 ctgacgttca gaccacggta gaaaacaaaa tcgagcgggc gaccaaacgc ccgacggcgc
     5341 tgatcatcgg taaaacgcac ctggcgcagc gacatttccc gcgcaaagcg atataacgcg
     5401 ttcatccttc tacggctcca ggcattgaaa tctcccgcca taatgaccgg gccgctgtgg
     5461 tgagctatct gatcaccaat aggaagtaac tgcttactat agacatccac gcccagactg
     5521 aagttgacgg cgtgtatatt aaccaccatc aacaggcggg tgtcaggtaa tggatagacc
     5581 gtcaccagtg ccgacttcgc cagacgcaaa atgggttctc gttcacgtaa cgggcagcaa
     5641 tacactggat gtgccgccga aagggtcatt acgccagaag gatgttgtgg cagcacgaaa
     5701 gcgggtacct gatcggcggc aagatagtta gcggtcgcaa actgtaccaa ctctggcgtt
     5761 gtttgcgctt cctgcaataa caccagatgt gcgtctttgc cgtaattctt taataccgac
     5821 aaccattcag cgcgttgctg tttgtatatg ttccacacca ggatccgaat acgctcttcg
     5881 gtacttaacg gttctccggg tggtaatgcc tggccgatgc tcgcaaaaga ccccggcggt
     5941 aagatcctct ccgcaggttg tccggcaaca tagcgcatgg cataggtgtt ttttcgcact
     6001 tttgactttg aaacctctat tacgtcaacc cttccgcact cgggagggtt ctactgttat
     6061 agggatttta gctcactctt tcaacgagca attgtatatt gttatgtaag caagtgctta
     6121 caccggtcag aaatttgctc attattgaga acattaaatc ggatgacata tcacgcatcc
     6181 tgatggttta atgacaaata aattcaaaat atctcatcta ttttgcaaca ggagcaaaaa
     6241 tgaaagccac gtcggaagaa ctcgccattt ttgtttcggt cgtcgaaagc ggcagcttta
     6301 gccgggcagc ggaacaatta gggcaagcaa actcagcggt aagccgggcg gtgaaaaagc
     6361 tggaaatgaa acttggcgtt agcctgctta atcggaccac gcggcaactt agcctgacgg
     6421 aagaaggcga gcgttatttt cgtcgcgtac agtcaatttt gcaggagatg gcagcggcag
     6481 aatcagaaat tatggagacg cgtaatacac cgcgtggact gttacggatc gatgccgcaa
     6541 ctccagtggt gctgcacttt ctgatgccgt taattaagcc tttccgtgaa cgctatccgg
     6601 aagtcacttt atcgctagtc tcctccgaaa cgattattaa tttgatcgaa agaaaagtgg
     6661 atgtcgcgat acgcgctggt acgttaacgg actccagctt acgtgccagg ccgttattta
     6721 acagttatcg aaaaattatc gcctcccccg attatatttc ccgctacggg aagccagaaa
     6781 cgatcgacga tttaaagcaa catgtttgcc tgggattcac tgaacccgct tccctcaata
     6841 cctggccgat agcctgtagt gatggacaat tacatgaggt gaagtacggt ttgtcatcca
     6901 atagtgggga aacactgaaa cagctttgcc tgagtggcaa cgggattgcg tgtttgtccg
     6961 actacatgat cgacaaagaa atcgctcgcg gagaattggt ggagttaatg gcagataaag
     7021 tgttgccagt ggaaatgcca ttcagtgccg tctattacag cgaccgtgcg gtaagtacgc
     7081 gcatccgggc ttttatcgat ttccttagcg agcatgtaaa aacagctccc ggaggagctg
     7141 tcagagaggc ttaatcccat tcaggagcca gaccttccgg gctaaccagg cggtcgttgc
     7201 aatccagtgc ggcgatcgct tttttatctt cggcatcaag ctgtaaattt tgtgccttaa
     7261 gattactttc caggttttta cgtttagtag aagaaggaat tactgagtaa ccttccccca
     7321 tagcccacgc cagaatcact tgtgccggag tcgcattgtg tttagctgca atacgagcaa
     7381 taacctcatc tttcagtgcc ttaccataag ccagcgtcat ataggaagtg atatgaatac
     7441 cgtgctgttt agcccaggca accactttac ggttttgcag ataaggagag agttcaatct
     7501 ggttggtagc gatgttttca gcgccaacag cagcaatcgc tttttccatc aacgggatcg
     7561 tgaagttgga aataccgatc tcacgtgtca gaccttgttt tttggcctcc agcagcgcct
     7621 gcataaactc ttcaacagag acttcatcgt ttggtgacgg ccagtggatt agcgtcagat
     7681 caacataatc ggtacgcaat ttttgcaggc tctcttttag actcgggatc aatttgtctt
     7741 tgctgagatt ttcaatccag attttagtgg tgatgtagag ttcatgacgt ggcacgccac
     7801 tttctgcaat cgcctgacct actgcggctt cgttatcata gatttgtgca gtatcaattg
     7861 cgcgataacc aagttcaagc gccgttttca cagatgaaat aacaacgtcg tctttgagac
     7921 ggaaagtacc taaaccaaat gcagggatag ccataatatg cctcttttaa tgctattttc
     7981 gttaactgag gtgattatgg gcttcagact gttgcggaaa aagagcataa aatgcagagg
     8041 atttttgcga ttctggcaat aatagatata cagacgaaaa aaaacctcga gcgttaactc
     8101 gaggcttctt aataagtggc ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca
     8161 ggcaggtatt ctaaccgact gaactaccgc tccaccgaat tcttttacaa ccaccggttt
     8221 tatgaccggc ttactgctta atttgatgcc tggcagttta tgaatcactt cgtgattcac
     8281 ccttcgggcc gttgcttcgc aacgttcaaa agctttcgct tttgtcctac tctcttatcg
     8341 agtagtgaac tgcctcaaag gtcagtgcca ttcttc
//
LOCUS       DAZRUF010000178         8227 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZRUF010000178 DAZRUF010000000
VERSION     DAZRUF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.178"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="ACHXOL_003207"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="ACHXOL_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR6810120.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHXOL_003208"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHXOL_003208"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR6810121.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="ACHXOL_003209"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="ACHXOL_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHXOL_003210"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHXOL_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR6810122.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHXOL_003211"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHXOL_003211"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR6810123.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="ACHXOL_003212"
     CDS             complement(6490..6846)
                     /locus_tag="ACHXOL_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR6810124.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="ACHXOL_003213"
     CDS             complement(6850..7266)
                     /locus_tag="ACHXOL_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR6810125.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHXOL_003214"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHXOL_003214"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR6810126.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         2045 a         2087 c         2050 g         2045 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aatcccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatat
//
LOCUS       DAZRUF010000179         8183 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZRUF010000179 DAZRUF010000000
VERSION     DAZRUF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.179"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..2848
                     /gene="yfaL"
                     /locus_tag="ACHXOL_003215"
     CDS             <1..2848
                     /gene="yfaL"
                     /locus_tag="ACHXOL_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR6810127.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2976..3698)
                     /gene="ubiG"
                     /locus_tag="ACHXOL_003216"
     CDS             complement(2976..3698)
                     /gene="ubiG"
                     /locus_tag="ACHXOL_003216"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR6810128.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3845..6472
                     /gene="gyrA"
                     /locus_tag="ACHXOL_003217"
     CDS             3845..6472
                     /gene="gyrA"
                     /locus_tag="ACHXOL_003217"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR6810129.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6680..>8183
                     /locus_tag="ACHXOL_003218"
     CDS             6680..>8183
                     /locus_tag="ACHXOL_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR6810130.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2032 c         2321 g         1851 t
ORIGIN      
        1 tgccgacgga aaaacgctgg ttattggcaa tacagagaat gacggagctg ttgactctat
       61 tgctggtacc gggttgatca ccaaaacagg ttccggcgat ctggtactta atgcagataa
      121 caatgacttt actggtgaga tgcagattga aaacggtgaa gttaccctgg gccgcagcaa
      181 ctccctgatg aatgtcggcg atacgcattg ccaggacgat ccgcaagact gctacggtct
      241 gacgataggg agtattgata agtaccagaa tcaggcagag ctaaacgttg gctccaccca
      301 acaaaccttt gtgcactcat tgacgggctt tcagaatggc actttaaata tcgatgctgg
      361 tggcaatgtt actgttaatc aaggcagttt tgctggcacc atcgaaggtg ctggtcagct
      421 caccattgcg caaaacggca gctacgtgct gtcgggggcg cagtcgatgg cgctaaccgg
      481 cgatatagtc gttgatgatg gtgcggtgct ttcgctggaa ggcgacgcgg cagatctcgc
      541 cgctctccag gacgatccgc agtcgatcgt gttaaacggc ggtgtgctcg atctctctga
      601 tttctccacc tggcagagcg gcacatcata caatgatggc cttgaagtca gtggcagcag
      661 cggaacggtt atcggcagtc aggatgtggt agatcttgca ggtggcgaca atttgcatat
      721 cggcggcgac gggaaagatg gcgtctacgt ggtggtcgac gcgagcgacg ggcaggtaag
      781 tctggcaaac aataatagtt atttgggcac aacacaaatc gcctccggta cgctgatggt
      841 gagcgacaac tcgcagcttg gagataccca ctataaccgc caggttatct ttaccgataa
      901 gcaacaagaa agcgtgatgg agattactgc caatgtcgat actcgctcta cgacgactga
      961 gcatgggcgt gatattgaaa tgcgcgccga cggtgaagtg gcagttgatg cgggggtaga
     1021 cacgcagtgg ggcgcactga tggctgacag cagcgggcag catcaggatg agggtagcac
     1081 atttactaaa acgggggcgg gtacgctgga gctgaccgcc agcggtacaa cgcagtcggc
     1141 agtacgtgtc gaagaaggca ccctgaaagg cgatgttgcg gatatctttc cttatgcttc
     1201 gtcactgtgg gtcggtgatg gggcggcgtt cgttactggc gcggatcagg atattcagtc
     1261 tatcgatgct acttccagcg gcactatcga tatcagcgat ggtacggttt tgcgtctgac
     1321 cgggcaggat acttccgtcg cccttaatgc ctcattgttt aacggcgatg ggacgctggt
     1381 gaatgctacc gatggcgtga cgttgacagg tgagcttaat accaaccttg aaactgacag
     1441 cctgacctat cttgccgacg tgacggttaa tggtgatctg accaatacgt ccggtgcggt
     1501 tagcctgcaa aatggcgtcg ctggcgatac gctgacggta aacggtgatt ataccggcgg
     1561 cggtacgcta ctgctcgata gcgaattaaa cggcgatgac tcggtaagcg atcaattggt
     1621 gatgaacggt aatactgctg gcaataccac ggtggtggtt aactctatta cagggattgg
     1681 tgagccgaca tcgacaggca ttaaagtggt tgatttcgca gctgatccca cgcagtttca
     1741 aaacaatgcg cagttcagtc tggcaggcag cggctacgtc aatatgggag cgtatgacta
     1801 cacgctggtg gaagataaca acgactggta tctgcgatcg caagaagtaa cgccgccatc
     1861 gccacctaat cccaacccga ctcccgatcc tgatcccaca ccggatcctg acccaacccc
     1921 cgacccggcc cctacgcctg cttaccagcc ggtgctgaat gccaaagttg gcggttatct
     1981 caataacctg cgggcggcaa atcaggcgtt tatgatggag cgacgcgatc acgctggtgg
     2041 cgatggtcag acgctgaatt tacgtgttat cggcggacgt tatcattaca cggcggcagg
     2101 gcaactggct cagcaggaag acacttctac ggtgcagctt agcggcggct tgtttagcgg
     2161 gcgctggggc acggatggcg agtggatgct tggggcggtt ggtagctaca gtgataatca
     2221 gggcgacagc cgctcgaata tgaccggaac tcgcgccgat aaccagaacc acggttatgc
     2281 cgttgggctg acctcaagct ggtatcagca cggtaatcag aagcaggggg cctggctgga
     2341 taactggttg cagtacgcgt ggtttagcaa tgatgtttct gaacatgaag atggcacaga
     2401 tcattaccat tcgtcgggga ttatcgcctc gctggaagcc gggtatcagt ggttaccggg
     2461 gcgtggtgtg gtgattgaac cgcaggcgca ggtgatttat cagggcgtgc agcaggatga
     2521 ttttaccgcc gctaaccatg cgcgcgtgtc acaatcgcag ggggatgata ttcagacgcg
     2581 gctgggttta cacagcgaat ggcgtaccgc ggttggtgtc acaccaacat tagatctgaa
     2641 ttattatcac gatccccatg cgacggaaat tgaagaagat ggcagcacta tcagtgacga
     2701 tgcggtgaag cgacggggtg aaataaaagt gggagtcacg ggcaatatca gtcagcgagt
     2761 gtccctgcgc ggcagcgtgg cgtggcagaa agggagtgat gattttgccc agacggcagg
     2821 gtttttgtcg atgacggtga aatggtaatc gctgtctgac catcacaatt gccggatgcg
     2881 gcgtaaacgc cttatctggc ctacggagag catttgtagg cctgataaga cgctgcaagc
     2941 gtcgcatcag gcattggcgc ggccaaacat caacctcact tattctgcgt gtgcagcata
     3001 tagttcacat ccacgccggg gccgagctta aaggaattag tgatcgggtt gtaatgtagc
     3061 ccggttatat gtcgctcttt taaactggtc tgatccaccc agccaagcag ctctgccggt
     3121 ttaataaact tcttcacatc atgcgtacct ttgggcacca tgcgcaaaat atattccgcg
     3181 ccaaccaccg ccatcagcca tgacttgccg ttgcggttaa gcgtcgagaa aaagacatcg
     3241 ccgcctggtt tcaccagttg cgcacaggct ctgaccactg actgcggatc gggtacgtgc
     3301 tccagcatct ccatgcaggt caccacatca tactgcccgg catgttttgc cgcgtgctct
     3361 tccacggttt cctgcacgta atccacctga atgccgcttt ccagcgcgtg cagctttgcc
     3421 acctgcaatg gctcaaagcc catatccaga ccggtcaccg tcgcgccttc gcgcgccata
     3481 ctctcggcca gaatgccgcc gccacaaccg acatcgagca cctttttgcc aaataaaccg
     3541 ccagcacgct cggcaatata gcccagacgc agcgggttaa tgcggtgcaa cggtttgaac
     3601 tcaccttcaa gatcccacca gcgggaggcg acggcttcaa atttagcgat ctcttcgtgg
     3661 tctacgttat ggttttccgg cgatttttcg gcattcattg gcacttctac tccgtaattg
     3721 gcaagacaaa cgagtatatc aggcattgga tgtgaataaa gcgtataggt ttacctcaaa
     3781 ctgcgcggct gtgttataat ttgcgacctt tgaatccggg atacagtaga gggatagcgg
     3841 ttagatgagc gaccttgcga gagaaattac accggtcaac attgaggaag agctgaagag
     3901 ctcctatctg gattatgcga tgtcggtcat tgttggccgt gcgctgccgg atgtccgaga
     3961 tggcctgaag ccggtacacc gtcgcgtact ttacgccatg aacgtactag gcaatgactg
     4021 gaacaaagcc tataaaaaat ctgcccgtgt cgttggtgac gtaatcggta aataccatcc
     4081 ccatggtgac tcggcggttt atgacacgat cgtccgtatg gcgcagccat tctcgctgcg
     4141 ttacatgctg gtagacggtc agggtaactt cggttccatc gacggcgact ctgcggcggc
     4201 aatgcgttat acggaaatcc gtctggcgaa aattgcccat gaactgatgg ccgatctcga
     4261 aaaagagacg gtcgatttcg ttgataacta tgacggcacg gaaaaaattc cggacgtcat
     4321 gccaaccaaa attcctaacc tgctggtgaa cggttcttcc ggtatcgccg taggtatggc
     4381 aaccaacatc ccgccgcaca acctgacgga agtcatcaac ggttgtctgg cgtatatcga
     4441 tgatgaagac atcagcattg aagggctgat ggaacacatc ccggggccgg acttcccgac
     4501 ggcggcaatc attaacggtc gtcgcggtat tgaagaagct taccgtaccg gtcgtggcaa
     4561 ggtgtacatc cgcgcccgtg ctgaagtgga agtcgacgcc aaaaccgggc gtgaaaccat
     4621 tatcgtccac gaaattccgt atcaggtaaa caaagcgcgc ctgatcgaga agattgcgga
     4681 actggtaaaa gaaaaacgcg tggaaggcat cagcgcgctg cgtgacgagt ctgacaaaga
     4741 cggtatgcgc atcgtgattg aagttaaacg cgatgcggtc ggtgaagttg tgctcaacaa
     4801 cctctactcc cagacccagt tgcaggtttc tttcggtatc aacatggtgg cattgcacca
     4861 tggtcagccg aagatcatga acctgaaaga tatcatcgcg gcgtttgttc gtcaccgccg
     4921 tgaagtggtg acccgtcgta ctattttcga actgcgtaaa gctcgcgatc gtgctcatat
     4981 ccttgaagca ctagccgtgg cgctggcgaa catcgacccg atcatcgaac tgatccgtca
     5041 tgcgccgacg cctgcagaag cgaaaactgc gctggtggct aatccgtggc agctgggcaa
     5101 cgttgccgcg atgctggaac gtgctggcga cgatgctgcg cgtccggaat ggctggagcc
     5161 agagttcggc gtgcgtgatg gtctgtacta cctgaccgaa cagcaagctc aggcgattct
     5221 ggatctgcgt ttgcagaaac tgaccggcct tgagcacgaa aaactgctcg acgaatacaa
     5281 agagctgctg gatcagatcg cggaactgtt gcgtattctt ggtagcgccg atcgtctgat
     5341 ggaagtgatc cgtgaagagc tggagctggt tcgtgaacag ttcggtgaca aacgtcgtac
     5401 tgaaatcacc gccaacagcg cagacatcaa cctggaagat ctgatcaccc aggaagatgt
     5461 ggtcgtgacg ctctctcacc agggctatgt taaatatcag ccgctttctg aatacgaagc
     5521 gcagcgtcgt ggcgggaaag gtaaatctgc cgcacgtatt aaagaagaag actttatcga
     5581 ccgactgctg gtggcaaaca cgcacgacca tattctgtgc ttctccagcc gtggtcgcgt
     5641 ctattcgatg aaagtttacc agttgccgga ggccactcgt ggcgcgcgcg gtcgtccgat
     5701 cgtcaacctg ctgccgctgg agcaggacga acgtatcacc gcgatcctgc cagtgaccga
     5761 gtttgaagaa ggcgtgaaag tcttcatggc gaccgctaac ggtactgtga agaaaaccgt
     5821 cctcaccgaa ttcaaccgtc tgcgtaccgc cggtaaagtg gcgatcaaac tggttgaagg
     5881 cgatgagctg atcggcgttg acctgaccag cggcgaagac gaagtaatgc tgttctccgc
     5941 cgaaggtaaa gtggtgcgct ttaaagagtc ttctgtccgt gcgatgggct gcaacaccac
     6001 cggtgttcgc ggtattcgct taggtgaagg cgataaagtc gtctctctga tcgtgcctcg
     6061 tggcgatggc gcaatcctca ccgcaacgca aaacggttac ggtaaacgta ccgcagtggc
     6121 ggaataccca accaagtcgc gtgcgacgaa aggggttatc tccatcaagg ttaccgaacg
     6181 taacggttta gtggttggcg cggtacaggt agatgactgc gaccagatca tgatgatcac
     6241 cgatgccggt acgctggtac gtactcgcgt ttcagaaatc agcatcgtgg gccgtaacac
     6301 ccagggcgtg atcctcatcc gtactgcgga agatgaaaac gtagtgggtc tgcaacgtgt
     6361 tgctgaaccg gttgacgagg aagatctcga taccatcgac ggcagtgccg cggaagggga
     6421 cgatgaaatc gctccggaag tggacgttga cgacgagcca gaagaagaat aattttactt
     6481 cttcatgcca aaagggagct atctcccttg tttgaattga aaagtccagg ctgcaaagtc
     6541 tgggcttttg tcgtattagg gcacggtaaa gtttggctgt gcccgtaaaa aatggctggc
     6601 tatacacaag gaatgtggca atgagtggtg aaaaaaagcg aaaggctggc ggttctatgg
     6661 tcttgtaggt tttggcgcaa tagtactgct ttccgctggt gtctgggcgt tgcaatatgc
     6721 tggcagtggg ccagaaaaaa cgttgtcgcc gctggtggtg cacaacaatc tgcaaatcga
     6781 tctcaatgag ccggacctct ttctcgacag cgactctctg agccagcttc ccaaagatct
     6841 cctcaccatt ccgtttctcc acgatgttct gagtgaagat ttcgttttct attatcagaa
     6901 tcatgccgat cgtctgggca ttgaaggcag cattcgtcgc attgtctatg aacacgatct
     6961 cacgctgaaa gataaactct tttcgtcact cttagatcag cccgcgcagg ctgcgctatg
     7021 gcacgataaa caaggccatc tttcacatta catggtgctg atccagcgca gtggtttaag
     7081 caaactgctg gagccattgt tgtttgccgc taccagcgac agccagttaa gcaaaacgga
     7141 aatcagtagc atcaagataa atagtgaaac tattcctgtt tatcagttgc gctataacgg
     7201 caataacgcc ctgatgttcg cgacttatca ggacaagatg ctggtgtttt ccagcacgga
     7261 tatgttgttt aaagatgatc agcaggatac cgaagccacg gcgatcgcag gtgatttgtt
     7321 gagcggcaaa aaacgctggc aagcaagctt tggcctggaa gagcgtgctg ctgaaaaaac
     7381 gccagtacgc cagcgcatcg tggtcagcgc caggttgctg ggttttggct accagcggtt
     7441 aatgccttct tttgctggcg tacgcttcga aatgggtaac gacggctggc acagttttgt
     7501 ggcgttaaat gatgaatccg ccagcgtaga taccagtttc gattttacgc cggtctggaa
     7561 cagtatgcct gccggggcca gcttctgtgt ggtggtgccg tattcacacg gtattgccga
     7621 agagatgctt tcgcacatca gccaggaaaa cgacaagctg aatggggcgt tagacggtgc
     7681 cgcggggctg tgctggtatg aagactcaaa attgcaaacc ccgctgtttg tcggtcagtt
     7741 tgatggcact gccgaacagg cgcaattgcc agggaaactg tttacgcaaa atattggtgc
     7801 gcacgaaagc aaagcgccag aaggtgtttt gccgataagc cagactcagc agggcgaagc
     7861 gcaaatctgg cgtcgcgaag tgagttcccg atacggtcag tatccgaaag cgcaggcggc
     7921 gcaacccgat caattaatgt cggattattt tttccgcgtg tcgctggcga tgcaaaacaa
     7981 aacgctgctt ttctccctcg atgacacgct ggttaataac gccctgcaaa cactgaataa
     8041 aaaccgcccg gcaatggtgg atgtaatacc cactgatggc atcgttccgc tctatatcaa
     8101 tccacaaggc gtggcgaaac tgctgcgtaa cgaaacgttg accagtctgc cgaagaatct
     8161 cgaaccggtt ttttataacg ccg
//
LOCUS       DAZRUF010000180         8158 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZRUF010000180 DAZRUF010000000
VERSION     DAZRUF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.180"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..311)
                     /locus_tag="ACHXOL_003219"
     CDS             complement(<1..311)
                     /locus_tag="ACHXOL_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810131.1"
                     /translation="MSQNDIIIRTHHKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     P"
     gene            836..2764
                     /gene="thrS"
                     /locus_tag="ACHXOL_003220"
     CDS             836..2764
                     /gene="thrS"
                     /locus_tag="ACHXOL_003220"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR6810132.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2768..3310
                     /gene="infC"
                     /locus_tag="ACHXOL_003221"
     CDS             2768..3310
                     /gene="infC"
                     /locus_tag="ACHXOL_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR6810133.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3407..3604
                     /gene="rpmI"
                     /locus_tag="ACHXOL_003222"
     CDS             3407..3604
                     /gene="rpmI"
                     /locus_tag="ACHXOL_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR6810134.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3657..4013
                     /gene="rplT"
                     /locus_tag="ACHXOL_003223"
     CDS             3657..4013
                     /gene="rplT"
                     /locus_tag="ACHXOL_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR6810135.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4130..4254
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4136..4180
                     /gene="pheM"
                     /locus_tag="ACHXOL_003224"
     CDS             4136..4180
                     /gene="pheM"
                     /locus_tag="ACHXOL_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR6810136.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4463..5446
                     /gene="pheS"
                     /locus_tag="ACHXOL_003225"
     CDS             4463..5446
                     /gene="pheS"
                     /locus_tag="ACHXOL_003225"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR6810137.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5461..7848
                     /gene="pheT"
                     /locus_tag="ACHXOL_003226"
     CDS             5461..7848
                     /gene="pheT"
                     /locus_tag="ACHXOL_003226"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR6810138.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7853..8152
                     /gene="ihfA"
                     /locus_tag="ACHXOL_003227"
     CDS             7853..8152
                     /gene="ihfA"
                     /locus_tag="ACHXOL_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR6810139.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
BASE COUNT         2129 a         1886 c         2173 g         1970 t
ORIGIN      
        1 aagggaatga tttctttttt atagtgtaat gtaatgagta tattttctgc atggatgggt
       61 gaagtggcaa agttaatgat aaaaagggca gtggaataac aattttgtga aatttttcga
      121 cagtttgcgg taaattcctg agtaacacag tatgacagca tcgaacttaa gtcagaggta
      181 tcaagtaggg tgatgagttg gcgcgcaaat tgattaatag gctctgatga aggcgttggt
      241 atgtcgctat cgatgtgcaa tctatgagga gacttatgat gggttctgat aatgatatcg
      301 ttttgactca taatctattc gccttatata tgtctggagg ttttgaggat gatgattaat
      361 tattatcgcc agaacgaaat gtgcacgaaa aattgctata agaattgtgt cacttttatc
      421 attgtgatat gaccacttcg tcattaatct catgagcgtt taaaatacta ccgccaaatc
      481 acgcaacgaa ctttgctgga ttcagtttat gctgctgtaa atccgctcgc agtaaacctt
      541 tcagacgcac cgtgatgtta tcagtggttc tttaagcgtt ttgctggtgt actcactaca
      601 aacgaattgc gaatcgaatc aatgtgaaac ggaaaggtac aatctccctt tttttagtgt
      661 taacatcgtt caaccgggtg gaaggtgaga ggttaatgtc tttcatttgt gtacctgatt
      721 gcgaaccaat ttagcatttg ttggctagat ggtttcgcaa tgaactgtta ataaacaaat
      781 ttttctttgt atgtgatctt tcgtgtgggt caccactgca aattaaggat ataaaatgcc
      841 tgttataact cttcctgatg gcagccaacg ccattacgac cacgctgtaa gccccatgga
      901 tgttgcgctg gacattggtc caggtctggc gaaagcctgt atcgcagggc gcgttaatgg
      961 cgaactggtt gatgcttgcg atctgattga aaacgacgca caactgtcga tcattaccgc
     1021 caaagacgaa gaaggtctgg agatcattcg tcactcctgt gcgcacctgt tagggcatgc
     1081 gattaaacaa ctttggccgc ataccaaaat ggcaatcggc ccggttattg acaacggttt
     1141 ttattacgac gttgatcttg accgcacgtt aacccaggaa gatgtcgaag cactcgagaa
     1201 gcggatgcat gagcttgctg agaaaaacta cgacgtcatt aagaagaaag tcagctggca
     1261 cgaagcgcgt gaaactttcg ccaaccgtgg ggagagctac aaagtctcca ttcttgacga
     1321 aaacatcgcc catgatgaca agccaggtct gtacttccat gaagaatatg tcgatatgtg
     1381 ccgcggtccg cacgtaccga acatgcgttt ctgccatcat ttcaaactaa tgaaaacggc
     1441 aggggcttac tggcgtggcg acagcaacaa caaaatgttg caacgtattt acggtacggc
     1501 gtgggcagac aaaaaagcac ttaacgctta cctgcagcgc ctggaagaag ccgcgaaacg
     1561 cgaccaccgt aaaatcggta aacagctcga cctgtaccat atgcaggaag aagcgccggg
     1621 tatggtattc tggcacaacg acggctggac catcttccgt gaactggaag tgtttgttcg
     1681 ttctaaactg aaagagtacc agtatcagga agttaaaggt ccgttcatga tggaccgtgt
     1741 cctgtgggaa aaaaccggtc actgggacaa ctacaaagat gcaatgttca ccacatcttc
     1801 tgagaaccgt gaatactgca ttaagccgat gaactgcccg ggtcacgtac aaattttcaa
     1861 ccaggggctg aagtcttatc gcgatctgcc gctgcgtatg gccgagtttg gtagctgcca
     1921 ccgtaacgag ccgtcaggtt cgctgcatgg cctgatgcgc gtgcgtggat ttacccagga
     1981 tgacgcgcat atcttctgta ctgaagaaca aattcgcgat gaagttaacg gatgtatccg
     2041 tttagtctat gatatgtaca gcacttttgg cttcgagaag atcgtcgtca aactctccac
     2101 tcgtcctgaa aaacgtattg gcagcgacga aatgtgggat cgtgctgagg cggacctggc
     2161 ggttgcgctg gaagaaaaca acatcccgtt tgaatatcaa ctgggtgaag gcgctttcta
     2221 cggtccgaaa attgaattta ccctgtatga ctgcctcgat cgtgcatggc agtgcggtac
     2281 agtacagctg gacttctcct tgccgtctcg tttgagcgcc tcctatgtgg gcgaagacaa
     2341 cgagcgtaag gtaccggtaa tgattcaccg cgcaattctg gggtcgatgg aacgtttcat
     2401 cggtatcctg accgaagaat tcgctggttt cttcccgacc tggcttgcgc cagttcaggt
     2461 tgttatcatg aatattaccg attcacagtc tgaatacgtt aacgaattga cgcaaaaact
     2521 atcaaatgcg ggcattcgtg ttaaagcaga cttgagaaat gagaagattg gctttaaaat
     2581 ccgcgagcac actttgcgtc gcgtcccata tatgctggtc tgtggtgata aagaggtgga
     2641 atcaggcaaa gttgccgttc gcacccgccg tggtaaagac ctgggaagca tggacgtaaa
     2701 tgaagtgatc gagaagctgc aacaagagat tcgcagccgc agtcttaaac aattggagga
     2761 ataaggtatt aaaggcggaa aacgagttca aacggcgcgc cctaaccgta tcaatggcga
     2821 aattcgcgcc caggaagttc gcttaacagg tctggaaggc gagcagcttg gtattgtgag
     2881 tctgagagaa gctctggaga aagcagaaga agccggagta gacttagtcg agatcagccc
     2941 taacgccgag ccgccggttt gtcgtataat ggattacggc aaattcctct atgaaaagag
     3001 caagtcttct aaggaacaga agaaaaagca aaaagttatc caggttaagg aaattaaatt
     3061 ccgtcctggt acagatgaag gcgactatca ggtaaaactc cgcagcctga ttcgctttct
     3121 cgaagagggt gataaagcca aaatcacgct gcgtttccgc ggtcgtgaga tggcgcacca
     3181 gcaaatcggt atggaagtgc ttaatcgcgt gaaagacgat ttgcaagaac tggcagtggt
     3241 cgaatccttc ccaacgaaga tcgaaggccg ccagatgatc atggtgctcg ctcctaagaa
     3301 gaaacagtaa ggccttcaag tagcactgtc tgtggagcct tcgggcttca caggctttgt
     3361 tcgcctttgt ttcgtttatt aacaatgcga agtggaagtt attaaaatgc caaaaattaa
     3421 gaccgtacgc ggtgctgcta agcgcttcaa aaaaaccggt aaaggtggtt ttaagcacaa
     3481 gcacgctaac ctgcgtcaca ttctgaccaa aaaagcgacc aaacgtaaac gtcacctgcg
     3541 tccgaaagcc atggtttcca aaggcgatct gggcctggta atcgcgtgcc tgccgtacgc
     3601 ataagccgtt aacgttttta actttttaat tagaatatag atacaggaga gcacatatgg
     3661 ctcgcgtaaa acgtggtgtt attgcacgcg cacgtcacaa gaaaattttg aaacaagcta
     3721 aaggctacta cggtgcgcgt tctcgcgtat accgcgttgc cttccaggct gttatcaaag
     3781 ctggtcagta tgcttaccgt gaccgtcgtc aacgtaagcg tcagttccgt caactgtgga
     3841 ttgcgcgtat caacgcagca gcacgtcaga acggtatttc ttacagcaaa ttcatcaatg
     3901 gcctgaaaaa agcctctgtt gaaatcgacc gtaagatcct ggctgatatc gcagtattcg
     3961 acaaagtagc gttcaccgct ctggttgaaa aagcgaaagc agctctggca taagccagtt
     4021 gaaagaggga gctagtctcc ctcttttcgt ttcaacgcca tcaaaacatt gacttttatc
     4081 gccgtagcct tttcaataaa ggtcttttga agagtaccaa aaggtaacgc aagcaatgaa
     4141 tgctgctatt ttccgcttct ttttttactt tagcacctga atccaggagg ctagcgcgtg
     4201 agaagagaaa cggaaaacag cgcctgaaag cctcccagtg gaggcttttt ttgtatgcgc
     4261 gttttgaaaa ataaaaagcc agtctatcag gcgtaattgt cgcagccagt ctggacacgg
     4321 ccagcgcgga gaaaccggag cgtatttaag tacgtgagaa tttcgagcac tgcccgggac
     4381 caaaatggca agtaaaatag cctgatggga taggctctaa gtccaacgaa ccagtgtcac
     4441 cactgacaca atgaggaaaa ccatgtcaca tctcgcagaa ctggttgcca gtgcgaaggc
     4501 ggccattagc caggcgtcag atgttgccgc gttagataat gtgcgcgtcg aatatttggg
     4561 taaaaaaggg cacttaaccc ttcagatgac gaccctgcgt gagctgccgc cagaagagcg
     4621 tccggcagct ggtgcggtta tcaacgaagc gaaagagcag gttcagcagg cgctgaatgc
     4681 gcgtaaagcg gaactggaaa gcgcagcgct gaatgcgcgt ctggcggcgg aaacgattga
     4741 tgtctccctg ctaggtcgtc gcattgaaaa cggcggtctg catccggtta cccgtaccat
     4801 cgaccgtatc gaaagtttct tcggtgagct tggctttacc gtggcaaccg ggccggaaat
     4861 cgaagacgat tatcataact tcgatgctct gaacattcct ggtcaccacc cggcgcgcgc
     4921 tgaccacgac actttctggt ttgacactac ccgcctgctg cgtacccaga cctctggcgt
     4981 acagatccgc accatgaaag cccagcagcc accgattcgt atcatcgcgc ctggccgcgt
     5041 ttatcgtaac gactacgacc agactcacac gccgatgttc catcagatgg aaggtctgat
     5101 tgttgatacc aacatcagct ttaccaacct gaaaggcact ctgcacgatt tcctgcgtaa
     5161 cttctttgag gaagatttgc agattcgctt ccgtccttcc tacttcccgt tcaccgagcc
     5221 ttctgcagaa gttgacgtca tgggtaaaaa cggtaaatgg ctggaagtgc tgggttgcgg
     5281 gatggtgcat ccgaacgtgc tgcgtaacgt tggcatcgac ccggaagttt actctggttt
     5341 cgccttcggg atggggatgg agcgtctgac tatgttgcgt tacggcgtca ccgacctgcg
     5401 ttcattcttc gaaaacgatc tgcgtttcct caaacagttt aaataaggca ggaatagatt
     5461 atgaaattca gtgaactgtg gttacgcgaa tgggtgaacc cggcgattga tagcgatgcg
     5521 ctggcgaatc aaatcactat ggcgggcctg gaagttgacg gtgtagaacc ggttgccggt
     5581 agcttccacg gcgtggtcgt tggtgaagtg gttgagtgtg cgcagcatcc gaacgctgac
     5641 aaactgcgtg tgacaaaagt gaatgtcggc ggcgatcgcc tgctggacat cgtctgtggt
     5701 gcgccaaact gccgtcaggg cctgcgtgtg gcggtagcta ccattggtgc tgttctgccg
     5761 ggtgatttca aaattaaagc ggcgaaactg cgtggcgaac cgtctgaagg gatgctgtgc
     5821 tccttctctg aactgggaat ttctgacgat cacagcggca ttatcgaact gcctgcagat
     5881 gcgccgattg gcactgacat ccgcgaatac ctgaaactcg atgacaacac catcgaaatc
     5941 agcgtaacgc caaaccgtgc cgactgctta ggtatcattg gtgttgcgcg tgacgttgcc
     6001 gtgctgaatc agctgccgct ggttgaaccg gaaatcgttc cggttggtgc gaccatcgac
     6061 gacacgttgc cgattacagt cgaagcgcct gaagcctgcc cgcgctatct tggccgtgtg
     6121 gtaaaaggca ttaacgttaa agcgccaact ccgctgtgga tgaaagaaaa actgcgtcgt
     6181 tgcgggatcc gttctatcga tgcagttgtt gacgtcacca actatgtact gctcgaactg
     6241 ggccagccga tgcacgcttt cgataaagat cgcattgaag gcggcattgt ggtgcggatg
     6301 gcaaaagagg gcgaaacgct ggtgctgctt gatggcactg aagcgaagct gaatgctgac
     6361 actctggtca tcgccgacca caacaaggcg ctggcgatgg gcggcatctt tggtggcgaa
     6421 cactctggcg tgaatgacga aacgcaaaac gtcttgctgg aatgcgcttt cttcagcccg
     6481 ctgtctatca ccggtcgtgc tcgtcgtcat ggcctgcata ctgatgcgtc tcaccgttat
     6541 gagcgtggcg ttgatccggc actgcagcac aaagcgatgg aacgtgcgac ccgtctgctg
     6601 atcgacatct gcggtggtga ggctggtccg gtaattgata tcaccaacga agcaacgctg
     6661 ccgaagcgtg caaccatcac tttacgtcgt agcaaactgg atcgcctgat cggccatcat
     6721 attgctgatg aacaggtaac tgacattctg cgtcgtctcg gctgcgaagt gaccgaaggc
     6781 aaagacgagt ggcaggcagt tgcgccgagc tggcgtttcg atatggagat tgaagaagat
     6841 ctggttgaag aagtcgcgcg tgtttacggc tacaacaaca tcccggatga gccggtacag
     6901 gcaagcctga ttatgggtac tcaccgtgaa gctgacctgt cgctcaagcg cgtgaaaacg
     6961 ctgctcaacg acaaaggcta tcaggaagtg atcacctaca gcttcgttga tccgaaagtg
     7021 cagcagatga tccatccagg cgttgaagcc ttactgctgc caagcccgat ctctgttgaa
     7081 atgtcagcaa tgcgtctttc tctgtggacc ggcctgctgg caaccgtggt gtacaaccag
     7141 aaccgtcagc agaaccgtgt gcgcattttc gaaagcggtc tgcgtttcgt accagatact
     7201 caggcaccgt tgggcattcg tcaggatctg atgttagccg gtgtgatttg cggtaaccgt
     7261 tacgaagagc actggaacct ggcaaaagag accgttgatt tctatgattt gaaaggcgat
     7321 cttgaatccg ttctcgacct gaccggtaaa ctgaatgagg ttgagttccg tgcagaagcg
     7381 aatccggcac tgcatccggg gcaatccgca gcgatttatc tgaaagggga acgtattggt
     7441 tttgttggag ttgttcatcc tgaactggaa cgtaaactgg atcttaacgg tcgcactctg
     7501 gtgttcgaac tggagtggaa caagctcgca gaccgcgtgg tgcctcaggc gcgcgagatt
     7561 tctcgcttcc cggcgaaccg tcgtgacatc gcggtggtgg tcgcagaaaa cgttcccgca
     7621 gcggatattt tatccgaatg taagaaagtt ggcgtaaatc aggtagttgg cgtaaactta
     7681 tttgacgtgt accgcggtaa gggtgttgcg gaggggtata agagcctcgc cataagcctg
     7741 atcctgcaag ataccagccg tacactcgaa gaagaggaga ttgccgctac cgtcgccaaa
     7801 tgtgtagagg cattaaaaga gcgattccag gcatcattga gggattgaac ctatggcgct
     7861 tacaaaagct gaaatgtcag aatatctgtt tgataagctt gggcttagca agcgggatgc
     7921 caaagaactg gttgaactgt ttttcgaaga gatccgtcgc gctctggaaa acggcgaaca
     7981 ggtgaaactc tctggttttg gtaacttcga tctgcgtgat aagaatcaac gcccgggacg
     8041 taacccgaaa acgggcgagg atattcccat tacagcacgg cgcgtggtga ccttcagacc
     8101 cgggcagaag ttaaaaagcc gggtcgaaaa cgcttcgccc aaagacgagt aatctgat
//
LOCUS       DAZRUF010000181         8157 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZRUF010000181 DAZRUF010000000
VERSION     DAZRUF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.181"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            116..511
                     /gene="caiF"
                     /locus_tag="ACHXOL_003228"
     CDS             116..511
                     /gene="caiF"
                     /locus_tag="ACHXOL_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR6810140.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(720..1310)
                     /gene="caiE"
                     /locus_tag="ACHXOL_003229"
     CDS             complement(720..1310)
                     /gene="caiE"
                     /locus_tag="ACHXOL_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR6810141.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1316..2101)
                     /gene="caiD"
                     /locus_tag="ACHXOL_003230"
     CDS             complement(1316..2101)
                     /gene="caiD"
                     /locus_tag="ACHXOL_003230"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR6810142.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2210..3763)
                     /gene="caiC"
                     /locus_tag="ACHXOL_003231"
     CDS             complement(2210..3763)
                     /gene="caiC"
                     /locus_tag="ACHXOL_003231"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR6810143.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            complement(3837..5054)
                     /gene="caiB"
                     /locus_tag="ACHXOL_003232"
                     /pseudo
     CDS             complement(3837..5054)
                     /gene="caiB"
                     /locus_tag="ACHXOL_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            complement(5183..6325)
                     /gene="caiA"
                     /locus_tag="ACHXOL_003233"
     CDS             complement(5183..6325)
                     /gene="caiA"
                     /locus_tag="ACHXOL_003233"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR6810144.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            complement(6356..7870)
                     /gene="caiT"
                     /locus_tag="ACHXOL_003234"
     CDS             complement(6356..7870)
                     /gene="caiT"
                     /locus_tag="ACHXOL_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR6810145.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
BASE COUNT         1963 a         2170 c         2021 g         2003 t
ORIGIN      
        1 cttatcgacg gtgaagttgc atactatcga tatatccaca attttaatat ggccttgttt
       61 aattgcttca aaacgagtca tagccagact tttaatttgt gaaactggag ttcgtatgtg
      121 tgaaggatat gttgaaaaac cactctactt gttaatcgcc gaatggatga tggctgaaaa
      181 tcggtgggtg atagcaagag agatctctat tcatttcgat attgaacaca gcaaggcggt
      241 taataccctg acttatattc tgtcggaagt cgcagaaata agctgcgcag ttaagatgat
      301 ccctaataag ctggaaggac ggggatgcca gtgccagcga ctggtaaaag tggtcgatat
      361 cgatgagcaa atttacgcgc gcctgcgcaa taacagccgg gataaattag tcggcgtaag
      421 aaagacgccg cgtattcctg ccgttccgct cacggaactt aaccgcgagc agaagtggca
      481 gatgatgttg tcaaagagta tgcgtcgtta atgtcatttc gttgatacca cgcagcctgg
      541 ttgctggatg caatgctggc gcatcttatc cagcctacgg ttgctctcca gccgggttgg
      601 ataaggcgtt tatgccgcat ccgacagcta tcgccggatg cgatgctggc gcatcttatc
      661 cggcctacgg ttgctctcca gcagagttgg ataaggcgtt tacgccgcat ccggcaagat
      721 caccgtttag gcgtcacatc cgtcgtcccc tgcaaacggg ggcgattttc ctccatttgc
      781 ctcagtggct gtgtttcatg taacgatgca tgacagcgcc cgacaagatc ctgatactct
      841 ttggtattca accgtttcca gtgtaactcg tcgtcactaa cactgcgtac agcgcgggct
      901 ggcgtaccca tcaacaactg gcgtttctcg ccgtgaaagc ctgctttgac aaagctcatg
      961 gcggcaacaa tgctctcttc gccaatgacc gcgccatcca taatcacgct gttcatcccg
     1021 accaatgcat cgcgaccaat cacacaacca tgcaggatcg ctccgtgccc gatatggccg
     1081 ttttccccaa cgatggtgtc agtgtcgcag tagccatgca taatgcagcc atcctgaata
     1141 ttggctcccg cttgcacgat caaccgcccg tagtcaccac gcagtgaggc gagtgggccg
     1201 atgtagacac cggctcccac aatcacatcg ccaatcaaga cggcactggg atggacaaac
     1261 gccgtcgggt gaaccaccgg aattaacccc tcaaaggcgt aatagctcac ggttgttaac
     1321 gtcctttcca caccggatcg cgcttctcgg caaacgccag cggcccttca atggcatcct
     1381 ccgaatgcag aaccgacgga tagtgtttca acacgccgct gcgaatatag cgatacgctt
     1441 cttctaccgg catttcgctg gtggtgcgga agatctcttt cagcgccgca atcgccagcg
     1501 gggcgctgtt aaccagctgc tgcgccagtt cgcgggcgtt atccatcagt tccgcctggc
     1561 taaccacgcg gttgactatc ccccaacgca gcgcctcttc tgcgcccatt cgtctgccgg
     1621 tcatcaccat ttcattgacg atggcaggcg gcaggatctt cggtagacgc agcacgccgc
     1681 cgctgtcagg aacgatgccc agcttggctt ccggcagggc gaagctggcg ttatcggcac
     1741 aaacaataaa atctgccgcc agcgccagtt cgaaaccgcc gccaaaggca tagccgttca
     1801 cagctgcgat aaccggtttg tcgagattga aaatttcggt taatcccgca aatccacccg
     1861 ggccaaagtc agcatccggt gcttcgcctt ctgctgccgc ttttaaatcc cagcccgcgg
     1921 aaaagaactt ctctccggca ccggtaataa tggcgacacg taattgcgga tcgtcacgga
     1981 aatttagaaa tacttcgccc atttcaaagc tggtttttgc atcaatagca ttcgcttttg
     2041 gacgatcaag ggtaatttcc agaattgatc cattgcgggt cagatgtaat gattcactca
     2101 ttccttttct ccatttttgc tttttcaggg acgacaacat ccctgcaaaa aatgcatatt
     2161 gttttatagc gtgattatta gttggcaggg tagttccctg ctgtttcatt tatttcagat
     2221 tctttctaat tattttcccc gagcaattac gtggcagatc ttttctgatc tccagataag
     2281 agggcacttt aaatttcgcc atattttgtt cgcagaagcg gaaaaattcc tcttcgctca
     2341 atgtttcacc ttcattcagc accacaaatg ctttgatggc ttcatcgcga atcgaatctt
     2401 taatacccac aaccacgata tcctgaattt ttggatgggt ggcgatgata ttttccagct
     2461 ccacgcagga gacattctcg ccaccgcgtt tgatcatatt gcagcggcga tcgacgaaat
     2521 aaaaaaagcc ctcttcgtcg cagtatccgg tatcgccggt atgtagccag ccgtcggctt
     2581 ccagcacttt cgcagtggct ttcgggttga gaaaatactc tttgaagatg gttttccctg
     2641 gtacgccttt aatacagatt tcaccgatct cacctgccgg gagcgggcga ttgtgatcgt
     2701 cgcggatctc cgcttcgtag caaaaccccg cccgaccaat cgacggccag cgtcgtttat
     2761 cgccagggcg atcgccgata atgcccacaa tggtttccgt catcccataa gacgtcagca
     2821 agcgaacgcc gaagcgttca caaaacgcat ctttttcctg ctccgacaag ttgagataaa
     2881 acatcacttc ccgcaggcgg tgttgccgat cgttcgctga aggcggctgc gccatcaagg
     2941 tacggatcat catcggaata cattcggtaa cggtggcgcg gtacttctgt acctgtcccc
     3001 agaaggcgcg ggcgctgtat ttctcgacca gcacaaaggt ggccccggca gaaaacgccg
     3061 ccatcgccgc agtacactgg caatcgatat gaaacgcagg cattaccgtc aggtagacgt
     3121 catcgtcacg cagtgcacac tgccaggcgg agtaatatcc agcgaagcgc aggttgtaat
     3181 gggtaatcac cacacctttc ggtcgggagg tggtgccgga ggtgaagaga atttccgccg
     3241 tatcgtcagt cgatagcggc ggtgcatagc acaaggtggc aggttgttga tttttcagtt
     3301 gagtaaacga actcacgcca tcatcagcgg gaagtgccac atctgtcagg caaatgtgcc
     3361 gcaattgagt ggcatcttcc tgctgaatct gttgatacat aggatagaat tgcgcactgg
     3421 tcaccagcag gcacgcctgg ctattttgca ggatccacgc gctttcctcg cgcaacaggc
     3481 gggcgttaat cggcaccata atcgcgccaa tttttgccag cccgaaccag caaaagataa
     3541 attccgggca gttgtcgaga tgtagagcaa ccttgtcgcc tttgcgaatc cccagcgtat
     3601 aaaacaggtt tgtcgtgcgg ttaatctcct gatttaactc aagataacta taccggttaa
     3661 cgactccgcc gctggattca caaatcagcg ccgttttatg accgtaaacg tccgccagat
     3721 cgtcccacat ttgacgtaga tgttgtccgc caatgatatc cattgcacct ctatccattt
     3781 ttgttcgttt gttattgggc gggcgctagt caggcaagcc gactgacgcc acgcgtttag
     3841 tcctcaactt tggccagacc tttgctgacc aactcctgaa tgtcgttttc gctgtagccg
     3901 atatttttca aaatggcagc cgtgtccatg ccatgtgagg gcattccgcg ccagatttgt
     3961 ccggggttat ttttgaattt cggcatgatg ttcggccctt tgcaggtgcg accatccatc
     4021 gtttgccact gagtgattga ttcacgggca acatactgtg gattgctttc cagttccggt
     4081 acggtcagta ctttggcaca ggcgatattc agttcggcaa agcgttcttt aacttctgcg
     4141 atggtatgtg ccgccagcca ggcatcgagt ttttcttcta ccagtgggcc gtaagggcat
     4201 tcgatacggt ggataagctg agtgccttcc gggatttccg gcgtaccgag tagatgtgcg
     4261 aggccaatat ctttaaagca ctcttcaatt tgggtaatgc ccaccagttc catcacgatg
     4321 tagccgtcgg cgcatttata cagaccgcaa ccggcgtagt agggatcttt acctttgctc
     4381 atgcgcgggc acatttcgcc gccgttgaag taatccatca tgaagtactg gcccatacgc
     4441 agcatcactt catacatggc gatgtcgata ctttcgcctt taccggtttc acgcacttta
     4501 tgcagtgctg ccagcgccgc cgtggtggcg gtcaggccag aaaagtaatc ggcggtatac
     4561 gggaaggcag gcattggctg gtcaacatca ccgttctgaa tcaggtaacc actgaaggcc
     4621 tgggcgatgg tgttataggc cggaagattg gtgtactcct cggtgccgta ctgaccaaaa
     4681 ccggacaggt gagcgataac cagtttcggg ttgtgctgcc acagtacttc atcggtaatg
     4741 ccacgacggg caaaggccgg acctttgctg gcttcgatga agatatcggt ggtttccatt
     4801 aatttcagaa acgcttcgcg gccttcatct ttgaaaatat ttaacgacag cgcgtgcaaa
     4861 ttgcggcggg agagctgcgg gtagttcggt tgaacgcgaa tggtgtcggc ctaggcgacg
     4921 ttctcgatcc agataacttc cgcaccccat tctgcgaaca tttgcccggc gaacggcccg
     4981 gcgatttcga taccggagaa gacaacgcgc aatccggcca acggcccgaa tttcggcatg
     5041 ggtagatgat ccattattag ctcctgaaaa atttatgtag cgcatgactg ccggatgcgg
     5101 cgtaaacgct ttatccggcc tacattcgtg ctcccgtagg cctgataaga cgcattagcg
     5161 tcgcatcagg cagcgcacgg acttagcggt attgcttcag caccgcgcga cccagcgtca
     5221 ggatctgcat ttcgtcagat cccccggaga cgcggtctac acgcagatca cgccagaagc
     5281 ggctgatgcg gtggttgccc gcaatcccga caccgcccag cacctgcatt gcgctatcca
     5341 caacttcaaa tgccgcattg gcgcagaagt atttgcacat cgctgcatcg ccagaggtga
     5401 tggtgccgtt gtctgctttc cacgctgctt catacagcat gtttttcatg gagtttaatt
     5461 tgatcgccat gtgggcgaat ttttcctgaa tcaactggaa acgaccaata gcctcgccaa
     5521 actgcacgcg ctgattggcg tagcgcgccg catcttcaaa agcgcacatc gccgtaccgt
     5581 agttggtgag ggctaccagg aaacgttcat ggtcgaactc ttctttgacg cggttaaagc
     5641 cgttaccttc ccgaccgaac atgtctttct cgtccagttc cacgtcgtca aaggtgattt
     5701 cacagcagct atccatacgc agaccgagct tttcaagttt ggtcactttg atgcccggtt
     5761 tgctcatatc aacaaaccat tcggtgtaga caggtttgtc cggagaagcc ccgtcgcgcg
     5821 ccatcaccac gatgtacggg gtgtaggcgc tgctggtaat aaaacactta ctaccattaa
     5881 gataaatctt accatttcta cgggtataag tcgttttcag gctacccacg tcggagcccg
     5941 cgcccggttc ggtaatcgct gagttccaca tctgcttacc ggtgccgcgg aaagccataa
     6001 ttttgtcgat ctgctcttgt gtgccttcgc gcaggaaggt gttgaacccg cccggcaact
     6061 ggtacagcac ataggttggt gcccccagac gtcccagctc catccacacg gcggcgagag
     6121 taacaaaccc cgcgtccaga ccaccgtgct cttcagggat cagcagactg tcgataccca
     6181 tatccgccag tgctttgaca aaacgttccg ggtagacgct gtcacggtcg cactcggcaa
     6241 aataggcctc ccagttttcg ctggccatca gttcgcggat accggcgaca aacagttcct
     6301 gctcatcatt taaattaaaa tccatctttc aacctcttga tattttgggg gttaattaat
     6361 ctttccagtt ctgtttcgcg tctttaataa aggagagcgt caccataatg ttgacgaaga
     6421 acagcgggca tcctccggcg ataatggcgg tttgaatcgg tttcaggccg ccgagcgcca
     6481 gcagaacaat accgataatg ccaaccagaa ttgaccaacc gatacgcacc agcagaggtg
     6541 gttcttcacc atcgcgtact tcgcggcaag tggacatcgc cagggtataa gagcaggcgt
     6601 taaccagcgt aacggtggca ataaagcaga ggatgaagaa gccccacatg gtggcggtgc
     6661 tgagtggcag agcggcccag gtttcaatga tggcgcgcgc cacaccgtac tgttcgatca
     6721 gatttggaat gttgatgatg tttttatcta tcaacagcag agtgttacta ccgagtacag
     6781 tccacaggat ccaggttgac gctgtcagcc ccagcaccat gccgaagcac agttcacgca
     6841 cagtacgacc acgggagatg cgggcgagga agatactcat ctggatagca taaatcaccc
     6901 accatgccca gtagaacacg gtccagccct gcgggaagcc gcctttagcg atgggatcgg
     6961 tatagaacaa catgcgcggc agatacatca gcaacatccc caccgaatcg gtgaagtagt
     7021 tcatgatgaa gctggcaccg ctgacaatga acacccaacc cagcatcagg aagctcaggt
     7081 aactacgcac gtcactggcg atacgtaccc ctttttgcag accgcaagcg acgcaaatgg
     7141 cgttgaggat aatccagcag gtaatgatga tagcgtccag ttgcagggta tgcggaatgc
     7201 caaacaacca ttgcatacac tcggtcacca gcggcgtggc aaggcccaga ctggtaccca
     7261 tcgcgaagat caaggcgacg agatagaagt tgtcgacgat agtgccgaac aaccctttgg
     7321 cgtgtttttc acctaccagc ggcaccagtg tcgagctggg gcgaatcact tccattttgc
     7381 ggacaaagaa gaagtaagcg aaggcgactg aaaggaagct gtaagtggcc cacggcagag
     7441 gtccccagtg gaacaagctg taagccagcc ccaactcttt cgcccctgtc gagttcggtt
     7501 ctaagccaaa cggcggggtg gagatgtagt agtagatctc aatcgatccc cagaacagta
     7561 cggcagcaga cgtacaggag gcgaacatca taaagatcca actggcggta ctaaattcag
     7621 gtggttcgtt acctaaacgc tttttggcat acgggccaaa caccagccag aaccaaccga
     7681 aaagcatcac caccatatac cattcaaatg cccatcccca tacattggtg acgtaactga
     7741 atacagcatt aataacgaca ttcgctgcat ccagatctct gactgtaagc caacaaagta
     7801 tgccgacgat tattaacggc ggaaagaaaa ccttcggttc tattcccgtt tttctctttt
     7861 cattcttcat gagttaattc cactgtgaaa acgaatattt attttgcgtt cccgtttgtt
     7921 ttatttttgt taacatttga tataattatt attaacctcg tggacgcgtt aatggctaac
     7981 tcataatggg tattcaataa gctgcattct gtgattgtta tcacattttt ttcgggtgaa
     8041 tagagggcgc ttttctcgtt aattttgatt attaatcagt ttgttatgtt gtgttgtgag
     8101 taaaaaaata gcatctgact ttcaatattg gtgatccata aagcaatatt gaaaatt
//
LOCUS       DAZRUF010000182         8157 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZRUF010000182 DAZRUF010000000
VERSION     DAZRUF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.182"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..254
                     /locus_tag="ACHXOL_003235"
     CDS             <1..254
                     /locus_tag="ACHXOL_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR6810146.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHXOL_003236"
     CDS             complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHXOL_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR6810147.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHXOL_003237"
     CDS             complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHXOL_003237"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR6810148.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2905..3180)
                     /locus_tag="ACHXOL_003238"
     CDS             complement(2905..3180)
                     /locus_tag="ACHXOL_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR6810149.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHXOL_003239"
     CDS             complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHXOL_003239"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR6810150.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4421..5140
                     /gene="trmB"
                     /locus_tag="ACHXOL_003240"
     CDS             4421..5140
                     /gene="trmB"
                     /locus_tag="ACHXOL_003240"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR6810151.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5140..5466
                     /locus_tag="ACHXOL_003241"
     CDS             5140..5466
                     /locus_tag="ACHXOL_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR6810152.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5650..6369
                     /locus_tag="ACHXOL_003242"
     CDS             5650..6369
                     /locus_tag="ACHXOL_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR6810153.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6545..7591
                     /gene="ansB"
                     /locus_tag="ACHXOL_003243"
     CDS             6545..7591
                     /gene="ansB"
                     /locus_tag="ACHXOL_003243"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR6810154.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7708..>8157
                     /locus_tag="ACHXOL_003244"
     CDS             7708..>8157
                     /locus_tag="ACHXOL_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR6810155.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
BASE COUNT         2088 a         1988 c         2160 g         1921 t
ORIGIN      
        1 ttcgcggtga agtggagttg gtgcggattc gtgatgccga agggcgaatt gcggcagaag
       61 gggcgttgcc ttatccacct ggcgtgcttt gcgtggttcc cggggaagtc tggggtgggg
      121 cggttcaacg ttatttcctt gcgctggaag aaggcgtgaa tttgttgccg ggattttcgc
      181 cggagttgca aggtgtctat agcgaaaccg atgcggatgg catgaaacga ttgtacggtt
      241 atgtgttgaa gtaagaataa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt
      301 aattagtggc taaccgtctg tgtgcctgtc gggacacgaa cgtgtttata tttgaacatc
      361 gccatgaacg cgaaggccag aaccacggaa taaccagcga aaatcaacca tacggtctgc
      421 cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg
      481 aggatacagc cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt
      541 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg
      601 caaccgtaaa cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg
      661 taagcaaaca gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta
      721 ataccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
      781 gaaatcgaca tgatgattga cgcatgctgc acgatgaagc tgctggcaaa catcggatct
      841 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
      901 agcagcattg agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca
      961 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
     1021 ggcagagtca gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc
     1081 atgtggctta attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg
     1141 gtgccccaga tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg
     1201 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
     1261 aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg ccgccatgaa cagcgtgata
     1321 gcgccaatgg tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca
     1381 atccccagca gcgcaggcat aaataccgcg gcgataccca gtgagctata aactgcgcca
     1441 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
     1501 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
     1561 ttaatttcct cacattgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
     1621 atgatgggat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
     1681 aacattccca atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc
     1741 gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta
     1801 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
     1861 cccggcgtca tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc
     1921 agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg
     1981 ttatcaattc cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca
     2041 atattgctgg caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag
     2101 cggaacacat ctttcccggc agtatgttgt accacctgca ttaatcccag cgcatcggaa
     2161 cggctgaccg catacgggtt aaaggaagat tcggtctgca taattgccag aatcagcgac
     2221 tcatcaacgc catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt
     2281 ttatcaaggt ggttcggtac catgttaatg gtgacgctgt agatgatacg cagcccgtta
     2341 ctgcggcttt gcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc
     2401 cagcgaatcg gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa
     2461 atcgtaatat catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcagc
     2521 aacgttttga taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatagtttcg
     2581 atagtaatcg taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat
     2641 ttcacgtagt ccttaggacc ggcgatcacc acctctttga atccccagat gttctcaata
     2701 ttgtgggcaa attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg
     2761 ttataggtat cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc
     2821 agcgcgagat attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagttgc
     2881 cggaggcgtg ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg
     2941 cacctcttta ccctcgaaca ggaagttgac catctcctgc tcaagcagct tgcggtgctc
     3001 ggcattcatc atgttgagtt tcttttcatt aatcagcatg gtttgcttgt gctgccactg
     3061 cgcccaggct tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag
     3121 ctgaaaatcc tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat
     3181 aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa
     3241 caaacgctcc acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag
     3301 cgcattgcct tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat
     3361 atctaagtgg aaatggctga aggtatgccg aaacgcggtc agttgcgtca ggttatcggc
     3421 agcaatctgc cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa
     3481 acagtataaa ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc
     3541 gtgctgtaac agcaaaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc
     3601 cggataaagc gaccagctat tattggcggc ggcaatacat ccgttttgta gcggacagag
     3661 cgaacatttc ggcttcgagc gcgtacaaat catcgcgccc aaatccatca tcgcctgatt
     3721 aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctc aaactccata atttattctc
     3781 gacctctttt ttcccaggcc agccgcttac agcatagcag cgcgccagca cgcgtttgac
     3841 gttaccgtcg agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt
     3901 ggaacgcccg acgcccggta acgccgcgac ttcttcaaag gtttccggga atttaccgcc
     3961 gtgtaaggtc gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc
     4021 aagcccggtc cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt
     4081 cgggaagcgc gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg
     4141 caacatcact tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagcgt
     4201 ttttcgcccg tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat
     4261 ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa
     4321 ccggaacttt ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa
     4381 tgcccgtttt cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc
     4441 accggaattt gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca
     4501 ggggcgactg accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt
     4561 tgagttcagc gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac
     4621 gcttgagatt ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccctga
     4681 gcaggacttc ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc
     4741 gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca
     4801 taaaatgatt cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca
     4861 caaagcgcgc cataataaac gccgtatcgt acaggtgccg tttgccgaac tggtaaaaag
     4921 caaactgcag ctggggggtg tattccatat ggcgaccgac tgggaacctt atgcggaaca
     4981 tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta
     5041 cgtgccgcgt ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg
     5101 tcacggagta tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt
     5161 cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg
     5221 cgattcccgg aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac
     5281 gaggttatcg aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt
     5341 ctgatctgca tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag
     5401 tggctggaag agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg
     5461 gactaagaaa gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca
     5521 ggcctggaaa gatgcacgat cgagtaggcg ggataaggtg tttacgccgc atccggcatg
     5581 gaaaacgcgt actttgttat caatctgggg ccagcaaatg ctggcctgat tttttcttga
     5641 gggaagacta tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc
     5701 gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa
     5761 accgtgcagg tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg
     5821 tataacggta agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag
     5881 gctgaactac gcagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgaaaaa
     5941 gcccgtattg cgctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc
     6001 agccgtctga ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg
     6061 cgcagcgatg gcctgacgtt tcactataaa gccattgatc aggttcgcgc cgaaggccag
     6121 caattagtga atcaggcaat gggtggaatt ttacaggaca gcattaatga aatgggcgcg
     6181 aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg
     6241 ctgcaatcct caatccaaac agagtggaaa aagcaggaaa aagatttcca gcagtttggc
     6301 aaagatgttt gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat
     6361 ttaaaataat cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt
     6421 tttgttacct gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta
     6481 acgtcaaatt tcccatacag agctaaggga taatgcgtag cgttcacgta actggaggaa
     6541 tgaaatggag tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc
     6601 agcattggca ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg
     6661 tgactccgca accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa
     6721 tgcggtgccg caactaaaag acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg
     6781 ctcccaggac atgaacgata atgtctggct gacactggcg aaaaaaatta acaccgactg
     6841 cgataaaacc gacggcttcg tcattaccca cggtaccgac acgatggaag aaaccgctta
     6901 cttcctcgac ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc
     6961 gtccacgtcc atgagcgcag acggtccatt caacctgtat aacgcggtag tgaccgcagc
     7021 tgataaagcc tccgctaatc gtggcgtgct ggtggtgatg aacgacaccg tactggacgg
     7081 tcgcgatgtc accaaaacca acaccaccga cgtagcgacc ttcaagtctg ttaactacgg
     7141 tcctctggga tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca
     7201 caccagcgat acgccattcg atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt
     7261 ttataactac gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga
     7321 tggcatcgtt agcgctggtg tgggtaatgg taacctgtat aaatccgtgt tcgacaccct
     7381 ggcaaccgcc gcgaaaaacg gcactgcagt agtgcgttct tcccgcgtac cgacgggtgc
     7441 taccactcag gatgctgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct
     7501 gaacccgcaa aaagcgcgcg tcctgctgca gctggctctg acgcaaacca aagatccgca
     7561 gcagatccag cagatcttca atcagtacta atcacctcgc cccggtatcg tgccggggct
     7621 ttttcacttc agacttacgt ccattgccaa ttttcattac cctaatgata atcaccgaaa
     7681 taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg
     7741 cttatgttga tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta
     7801 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa
     7861 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
     7921 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
     7981 acgaaagata aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct
     8041 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
     8101 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caattat
//
LOCUS       DAZRUF010000183         8134 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZRUF010000183 DAZRUF010000000
VERSION     DAZRUF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.184"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            90..1586
                     /gene="ravA"
                     /locus_tag="ACHXOL_003245"
     CDS             90..1586
                     /gene="ravA"
                     /locus_tag="ACHXOL_003245"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR6810156.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
     gene            1580..3031
                     /gene="viaA"
                     /locus_tag="ACHXOL_003246"
     CDS             1580..3031
                     /gene="viaA"
                     /locus_tag="ACHXOL_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR6810157.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3036..4028)
                     /gene="asnA"
                     /locus_tag="ACHXOL_003247"
     CDS             complement(3036..4028)
                     /gene="asnA"
                     /locus_tag="ACHXOL_003247"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR6810158.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            4180..4638
                     /gene="asnC"
                     /locus_tag="ACHXOL_003248"
     CDS             4180..4638
                     /gene="asnC"
                     /locus_tag="ACHXOL_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR6810159.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4728..5171
                     /gene="mioC"
                     /locus_tag="ACHXOL_003249"
     CDS             4728..5171
                     /gene="mioC"
                     /locus_tag="ACHXOL_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR6810160.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5550..7439
                     /gene="mnmG"
                     /locus_tag="ACHXOL_003250"
     CDS             5550..7439
                     /gene="mnmG"
                     /locus_tag="ACHXOL_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR6810161.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7503..8126
                     /gene="rsmG"
                     /locus_tag="ACHXOL_003251"
     CDS             7503..8126
                     /gene="rsmG"
                     /locus_tag="ACHXOL_003251"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR6810162.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2034 a         2084 c         2083 g         1933 t
ORIGIN      
        1 cccccctttg agactatttt agccaccagc aagaaacgtc tatactcgca atttacgcag
       61 aacttttgac gaaaggacgc cacttcatta tggctcaccc tcatttatta gcggaaagaa
      121 tttcccgcct gagcagttcg ttggaaaagg ggctttatga acgtagccac gccatccgct
      181 tgtgtttatt agcggcatta agtggtgaaa gtgtgttcct ccttggcccg ccaggtattg
      241 ccaaaagttt gatcgcccgg cgcttaaaat tcgcctttca gaatgcccgc gcgtttgaat
      301 atctgatgac ccgcttctcc acgccggaag aagtttttgg tcccctttct attcaggcgc
      361 taaaagatga agggcgctat gaacgtttaa ccagcggtta cctgccggaa gcagaaattg
      421 tctttctgga tgagatctgg aaagcgggcc cggcaattct taataccttg ctcaccgcca
      481 ttaacgagcg ccagttccgc aacggtgcac acgtagaaaa aatcccgatg cgtctgctgg
      541 tggcggcctc caacgagctg ccggaagcag acagcagtct ggaagcgtta tatgaccgca
      601 tgctgattcg tctgtggtta gataaagtgc aggataaagc gaatttccgc tccatgctga
      661 ccagtcaaca ggatgaaaac gacaatccgg ttcctgatgc cttgcaggtc acagatgaag
      721 aatatgaacg ctggcagaaa gagattggtg aaattacgct gcccgatcat gtatttgagc
      781 tgatttttat gctgcgccag caactggata aattaccgga tgcgccttat gtctcggatc
      841 gtcgctggaa aaaagcgatc cgattattgc aggccagcgc cttttttagc ggtcgcagtg
      901 ctgttgcccc ggtagatctc attttgctga aagattgcct gtggtatgac gcgcaaagcc
      961 tgaatttgat acaacaacaa attgatgtat tgatgaccgg tcatgcctgg caacagcaag
     1021 ggatgttgac tcgccttggc gcgattgtgc aacgtcacct gcaactccag cagcaacaaa
     1081 gcgataaaac agccttaacg gtaattcgtc tgggcggcat tttcagccgt cgtcagcagt
     1141 atcaacttcc tgttaacgtt actgcttcca ctctgactct actgctgcaa aaaccgttaa
     1201 aactgcatga tatggaagtg gttcatatct cctttgagcg tagcgcgctg gaacagtggc
     1261 tgagcaaagg tggtgaaatt cgcggtaaac tgaacggtat cggctttgcc cagaaactga
     1321 atctggaagt tgatagcgcc caacatcttg tcgttcgcga tgtaagttta caaggcagta
     1381 cgctggcact tcccggttca tcggctgaag gtctgcctgg tgaaataaaa caacaactgg
     1441 aagagcttga aagcgactgg cgcaagcaac acgctttatt cagcgaacag caaaaatgtc
     1501 tgtttatccc tggcgactgg ttaggtcgca ttgaagccag cctacaggat gtcggtgcac
     1561 agattcacca ggcacaacaa tgctaacgct ggatacgctt aatgtgatgc tggccgtcag
     1621 cgaagaggga ttgatcgaag agatgatcat cgcactgctg gcctcaccgc agctggcggt
     1681 cttctttgaa aaattcccac gcctgaaggc ggcaatcact gatgatgttc cccgctggcg
     1741 tgaggcgctg cgcagtcggc tgaaagatgc ccgagtcccg ccggaactca ccgaagaggt
     1801 gatgtgctat cagcaaagcc agctcctctc cacgccgcag tttattgtgc agctaccaca
     1861 gatcctggac ttactgcatc gtctgaattc cccatgggca gaacaagccc ggcagttggt
     1921 tgatgctaac agcacaataa cttcagcgtt acacacgctt tttctccagc gttggcgttt
     1981 aagtctgatc gtgcaagcaa cgacgttaaa tcaacagcta ttagaagaag aacgcgaaca
     2041 actgttgagt gaagttcaag aacgcatgac gctgagcgga caacttgaac cgattctcgc
     2101 agataacaat actgcagctg gtcgtctgtg ggatatgagc gccggtcagc ttaaacgtgg
     2161 cgactatcag ttgattgtga aatacggtga atttcttaac gaacagccgg aactgaaacg
     2221 cctggcagag cagctggggc gttctcggga agccaaatca ataccgcgca acgatgcgca
     2281 gatggaaacc ttccgcacca tggtgcgcga accggcgacg gttcctgagc aggttgatgg
     2341 tctgcaacaa agcgatgata ttttacgtct tctgccgcca gaactggcga cactagggat
     2401 aacggaactg gagtatgagt tttaccgtcg gctggtggaa aaacagttgc tcacctatcg
     2461 cctgcacggt gagtcgtggc gtgaaaaagt gatcgaacgt ccggtggtac ataaagatta
     2521 cgatgaacag ccgcgcgggc cgtttattgt ctgtgtggat acttccggct caatgggcgg
     2581 ctttaatgaa cagtgtgcga aagcgttctg cctggccttg atgcgcattg ctctcgctga
     2641 aaaccggcgc tgctatatta tgctattttc caccgagatc gtccgttatg agctttcagg
     2701 cccccaaggc atcgaacaag caatccgttt tttaagccag cagtttcgtg gcggcaccga
     2761 tcttgccagt tgttttcgcg ccattatgga acgcttgcaa agcagggaat ggtttgatgc
     2821 cgatgcggtg gtgatttctg attttatcgc tcagcggttg cctgacgacg tgacgagtaa
     2881 agtgaaagag ctgcagcggg tacatcagca tcgctttcat gccgtggcga tgtcggcaca
     2941 cggcaaaccc ggcatcatgc gcattttcga tcatatctgg cgctttgata ccgggatgcg
     3001 aagccgcctg ctcagacgct ggcggcgata aattattaca gcagagaagg gacgttctcg
     3061 cgaacagcag ctggccatac accacactga acctggccga tatgcggcag ttgcagcagc
     3121 agcatggtca aacgagactg gccgataccg ccgccgatgg tctgcggcat ttcaccgcgc
     3181 agcagcgcct gatgccattc cagctgcaga cgatcttcat caccggtcag cgccaactga
     3241 tgcttcaacg tgtcggcatc cacgcggatc cccatggaag aaagctcaaa cgcatcttcc
     3301 agtacagggt tccacaccag aatatcgccg ttcagacccg catgacccag ctctgacggg
     3361 gtgctccagt catcataatc cggtgcgcgc acgtcgtggc gatgaccatc gctcagcttg
     3421 ccgccaatcc cgacaaggaa taccgcgcca agatctttcg ctatcgcccg ctcacgccct
     3481 ttggcatcga gatccggata acgagacagt aactcctggc tgtgtacgaa gtggatctga
     3541 tccggcagga acggtgccag gccaaactct tcgctaaccg cagcttcggt tgctttaatt
     3601 cccgcccaga tcgcctctac cgtgcttttc agagtcgaga attgacgctc accgtcgccc
     3661 attacgcgtt cccagtccca ctggtcaaca tagaccgagt gcaacggaga aagacggtct
     3721 tcatcggggc gaagggcttt catgtgcgtg tacagccctt cgcccgcgct gaagtcgtgt
     3781 tgccctaagg tctgacgttt ccacttcgcc agtgaatgaa ccacttcgaa ctgggcatca
     3841 ggcagagctt tcacttttac ctgcaccgct ttttcacagc ccgacaagtt atcctgcgtg
     3901 ccatccccca cacggctaag aatcggcgcc tggacttcga tcagccccag acgttcttcc
     3961 agttggcgag aaaagtgaga tttcacgaag ctaatttggc gttgtttggc aatgtaagcg
     4021 gttttcattt tttatactcc tgcgtcctgt tgcttatgat taagcaacaa aaaccgacaa
     4081 acatgcaata atcattcaat aaaaagccta acacactttt gattcattaa aaaagaaggc
     4141 taaaatagaa tgaatcatca atctgcataa gaaaatccta tggaaaatta tctgatcgac
     4201 aatctggacc gtggcatcct ggaagcatta atgggcaatg cgcgcaccgc ttacgccgaa
     4261 ctggcgaaac aatttggcgt cagtccgggg acgattcacg ttcgagtaga gaaaatgaag
     4321 caggcgggga tcattaccgg ggcgcgtatt gatgtcagcc cgaagcagct cggttatgac
     4381 gtaggctgct ttatcggcat tatattaaag agcgccaaag actacccttc cgcgctggca
     4441 aagctggaaa gccttgctga agtcactgaa gcctattaca caacaggcca ctacagcatc
     4501 tttataaaag taatgtgccg ttcgatcgac gctctccagc atgtacttat caacaagatc
     4561 caaacaattg atgaaattca gtccaccgag acattgatcg tcctgcaaaa cccgatcatg
     4621 cgtaccatca agccataatc ggctttttta atcccatact tttccacagg tagatcccaa
     4681 cgcgttcaca gcgtacaata cgccactctt aataaaggtg gcggtttatg gcagatatca
     4741 ctcttatcag cggcagcacc ctcggcggtg ccgaatatgt agcagaacac ctggctgaaa
     4801 agctggaaga ggcgggtttt accaccgaaa cgctgcacgg tccgctgttg gaagatttat
     4861 ctgcctcggg gatctggctg attatcagct ccacccacgg tgccggagat attccggaca
     4921 acctttctcc tttctatgaa gcattgcagg aacagaagcc cgatctttct gcggtccgct
     4981 ttggcgcaat cggtattggt agtcgtgaat atgacacctt ttgtggggct atcgataaac
     5041 tcgaggcaga actcaaaaat tccggtgcaa aacagacagg cgaaacactg aagatcaaca
     5101 ttcttgatca cgacattccg gaagatccgg cagaagaatg gctgggatcg tggattaatt
     5161 tactcaaata agtatacaga tcgtgcgatc tactgtggat aactctgtca ggaagcttgg
     5221 atcaaccggt agttatccaa ggaacaacgg ttgttcagtt tttgagttgt gtataacccc
     5281 tcattctgat cccagcttat acggtctagg atcaccgatc attcacagtt aatgatcctt
     5341 tccaggttgt tgatcttaaa agccggatcc ttgttatcca cagggtagtg cgatcctaat
     5401 aagagatcac aatagaacag atctctaaat aaatagatct tctttttaat acccaggatc
     5461 ccaggtcttt ctcaagccga caaagttgag tagaatccac ggcccgggct tcaatccatt
     5521 ttcataccgc gttatgcgag gcaatcacca tgttttatcc ggatcctttt gacgtcatca
     5581 tcattggcgg gggtcatgca ggcaccgagg ccgcgatggc cgcggcgcgt atgggtcaac
     5641 agactctgct tttgacacac aatatcgaca ctctggggca gatgagctgc aacccggcga
     5701 tcggcggtat tgggaaggga catctggtaa aagaagtgga tgcactcggc ggtctgatgg
     5761 cgaaagcgat cgatcaagcg ggtatccagt ttaggatact aaacgcaagc aaaggaccgg
     5821 cggttcgcgc tacccgagct caggcggatc gtgtgctcta ccgtcaggcg gtacgtacgg
     5881 cgctggagaa ccaaccgaac ctgatgatct tccagcaggc ggttgaagat cttattgtcg
     5941 aaaacgatcg cgtggtcggt gctgttaccc aaatgggact gaagttccgt gccaaagccg
     6001 tcgtgctcac cgttgggacg ttcctcgacg gtaaaattca tatcggtctg gataattaca
     6061 gcggtggtcg tgctggtgat ccgccgtcca ttccgctttc tcgccgtttg cgtgaactgc
     6121 cgctgcgcgt tggtcgtctg aaaaccggga caccaccgcg tattgatgct cgaactatcg
     6181 actttagtgt gctggcgcaa cagcatggcg ataacccaat gccggtattc tcgtttatgg
     6241 gcaatgcgtc ccagcatccc cagcaggtgc cgtgttatat cactcatacc aacgagaaaa
     6301 cccatgatgt gatccgcagt aacctcgatc gtagcccaat gtacgcaggg gtgatcgaag
     6361 gtgtcggccc acgctactgc ccatcgatcg aagacaaagt catgcgcttc gccgacagaa
     6421 atcagcatca gatcttcctt gaaccggaag ggctgacctc taacgaaatt tatccgaacg
     6481 gtatctccac cagcctgccg ttcgatgtgc agatgcaaat cgtccgctcc atgcagggga
     6541 tggaaaacgc gaagatcgtg cgtccgggtt atgccattga gtatgacttc ttcgatccac
     6601 gcgacctgaa accgacgctg gagagcaagt ttatccaggg gctgttcttt gctggtcaga
     6661 ttaacggcac taccggttac gaagaagccg ctgcgcaagg tttgctggtc ggtcttaacg
     6721 ctgcccgtct gtctgctgac aaagaaggtt gggctccggc gcgttctcag gcgtatctcg
     6781 gcgtactggt tgatgacctg tgcactttag gaaccaaaga accgtatcgt atgtttactt
     6841 cgcgcgcaga atatcgtctg atgctgcgcg aagataatgc ggatctgcgt ttgactgaaa
     6901 tcggtcgtga actgggcctg gtggatgacg aacgttgggc gcgctttaac gagaaacttg
     6961 agaatatcga gcgtgagcgt cagcgtctga aatcgacctg ggtaaccccg tcggcggaag
     7021 ctgcagccga agtgaatgct cacctgactg cgccgctttc ccgtgaagcc agtggtgaag
     7081 atctgctgcg tcgtccggaa atgacttatg aaaaattaac cacgctgacg ccgtttgccc
     7141 ctgcgttgac agacgaacag gcggcggaac aggttgagat tcaggttaaa tacgaaggtt
     7201 atatcgcgcg ccagcaagat gagatcgaaa agcagctgcg taacgagaac accctgctac
     7261 ccgcgacact ggattaccgc caggtatccg gtctttctaa cgaagtgatc gccaaactta
     7321 acgatcacaa accggcctct atcggccagg cttcgcgtat ttctggcgtc acgcctgcgg
     7381 ctatctccat tctgctggtg tggctgaaaa aacagggtat gctgcgtcgt agcgcataac
     7441 gcattaaaaa tgcctggtaa gcacccgctt accaggcaac gcatcaagaa caggtaatca
     7501 ccgtgctcaa caaactctcc ttactgctga aagacgcagg tatttcgctt accgatcacc
     7561 agaaaaacca gcttattgcc tacgtgaata tgctgcataa atggaacaaa gcgtacaacc
     7621 tgacttcggt ccgcgatcct aatgagatgc tggtacgcca tattctcgat agcattgtgg
     7681 tggcaccgta tctgcaaggt gaacggttta tcgatgtcgg caccggacca ggactgccag
     7741 gcattccact ctctatcgtg cgtcctgaag cccatttcac tctgttggat agccttggta
     7801 aacgcgtgcg tttccttcgt caggtgcaac atgagcttaa actggagaat attgagccag
     7861 tacagagcag ggtagaagag tttccttcag agccgccatt tgatggcgta attagccgcg
     7921 cttttgcctc tctgaacgat atggtgagct ggtgccacca tcttcctggt gagcaaggcc
     7981 gtttctacgc gctgaaaggg caaatgccgg aagatgaaat cgctttgttg cccgaagaat
     8041 atcaggtcga atcagtggtt aaacttcagg ttccagctct ggatggcgaa cgtcatctgg
     8101 tggtgattaa agcaaataaa atttaatttt tatc
//
LOCUS       DAZRUF010000184         8039 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZRUF010000184 DAZRUF010000000
VERSION     DAZRUF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.185"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..188
                     /locus_tag="ACHXOL_003252"
     CDS             <1..188
                     /locus_tag="ACHXOL_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810163.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
     gene            411..908
                     /gene="ubiC"
                     /locus_tag="ACHXOL_003253"
     CDS             411..908
                     /gene="ubiC"
                     /locus_tag="ACHXOL_003253"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR6810164.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            921..1793
                     /gene="ubiA"
                     /locus_tag="ACHXOL_003254"
     CDS             921..1793
                     /gene="ubiA"
                     /locus_tag="ACHXOL_003254"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR6810165.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHXOL_003255"
     CDS             complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHXOL_003255"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR6810166.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHXOL_003256"
     CDS             4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHXOL_003256"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR6810167.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5020..5628
                     /gene="lexA"
                     /locus_tag="ACHXOL_003257"
     CDS             5020..5628
                     /gene="lexA"
                     /locus_tag="ACHXOL_003257"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR6810168.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5701..7026
                     /gene="dinF"
                     /locus_tag="ACHXOL_003258"
     CDS             5701..7026
                     /gene="dinF"
                     /locus_tag="ACHXOL_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR6810169.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7142..7351
                     /locus_tag="ACHXOL_003259"
     CDS             7142..7351
                     /locus_tag="ACHXOL_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR6810170.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHXOL_003260"
     CDS             complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHXOL_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR6810171.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1802 a         1972 c         2211 g         2054 t
ORIGIN      
        1 atatgttgaa tcctacaaaa gataccaatt ggaattccac gtgtatctat aaatccagac
       61 ataagatgtt gcctattaat cttactcagg aaacactttt tagctccaaa tctcatggta
      121 aatatgcgct tttcccaatt tttactgcga gttggcgagc tactcgtata aagaatatag
      181 gtatttaagt aaaggaaaac atcaccgttc ctggcatcat gggcggtgat gcccctacgg
      241 ttgccctcgc cagcacgggc atcggcaaag cgtaaggttc aacagcgttt taccacttca
      301 tgcgattgtt gcgtttttgt tgcgcattag atcacttaat ttgctttaca tctcccgtaa
      361 acacttttct gcgatacaat gcctttacgt tatgtaacgg agagttcggc atgtcacacc
      421 ccgcgttaac gcaactgcgt gcgctgcgct attttaaaga gatccctgcc ctggattcgc
      481 aactgctcga ctggctgttg ctggaagatt ccatgacaaa acgttttgaa cagcagggaa
      541 aaacggtgag cgtgacgatg atccgcgaag ggtttgtcga gcagaatgaa atccccgaag
      601 aactgccgct gctgccgaaa gagtctcgtt actggttacg tgaaattttg ttatgtgccg
      661 atggtgaacc gtggcttgcc ggtcgtaccg tcgttcctgt gtcaacgtta agcgggccgg
      721 agctggcgtt acaaaaattg ggtaaaacgc cgctaggacg ctatctgttc acatcatcga
      781 cattaacccg ggactttatt gagataggcc gtgatgccgg actgtggggg cgacgttccc
      841 gcctgcgatt aagcggtaaa ccgctgttgc taacagaact gtttttaccg gcgtcaccgt
      901 tgtactaaga ggaaaaaaat atggagtgga gtctgacgca gaataagctg ctggcgtttc
      961 atcgcttaat gcgtacggat aagccaattg gcgcgttact gctgctctgg ccaacattat
     1021 gggcgttgtg ggtggcgaca ccgggcgttc cccagctctg gatcctggcg gtgtttgtcg
     1081 cgggtgtctg gctgatgcgc gctgccggat gtgtggtgaa tgattatgct gaccgcaagt
     1141 ttgatggtca tgttaagcgc acggcgaacc gaccgcttcc cagcggcgcg gtaacggaga
     1201 aagaggcgcg cgcgctgttt gtagtgctgg tattgatttc atttttactg gtgctgacgc
     1261 tgaatacgat gaccattctg ttgtcgattg ccgctctggc gttggcgtgg gtgtacccgt
     1321 ttatgaagcg gtatacccat ctcccgcaag tggtgctggg cgcggcgttt ggctggtcga
     1381 ttccaatggc ttttgccgct gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc
     1441 tcgccaatat tctctgggcg gtggcttacg acacgcagta tgcaatggtt gaccgtgacg
     1501 atgatgtgaa gattggtatt aaatccaccg caatcctgtt cggccaatac gataaattga
     1561 ttattggtat tttgcagatt ggcgtactgg cactgatggc gatcatcggt gagttaaatg
     1621 gcttaggctg gggatattac tggtcaattc tggtggctgg cgcgctgttt gtttatcaac
     1681 aaaaactgat tgccaaccgc gagcgtgaag cctgctttaa agcatttatg aataataact
     1741 atgttggtct ggtactgttt ttaggactgg caatgagtta ctggcatttc tgatgatgta
     1801 aaaaagccgg atgatcatcc ggctttcttc tgggttgcct gatgcgctgc gcttctcagg
     1861 cctacacaac acatcgcaat ttattgaatt tgcagattat ggaaggccgg ataaggcgtt
     1921 ttcgccgcat ccggcaattc tctctgatta cccttcgccc tgcgtcgcac tctcaatcgt
     1981 caaacgcacg tctgatgtaa tcaactccgc cagcaactga taaaccttca tcgtttctgc
     2041 cggttcggcg tcgccgctat cgctgatata cccttcatca cgcagcgtca gcaccagaga
     2101 actgaacacc gccttgtcga agaactccgg cgcgttgatg ccgtgcagca cggagagacg
     2161 ttgcgcaacg gtgcggctct ctttctccag cgtaccgcgg ttgatcgacg ggttggcact
     2221 caacaaccag aaggtgatgg cataacgttg cagcgtttcg cgcgcgcctg cggccagcag
     2281 ctgtaggggg cgagaatgcg ccgggttgat atgcaactca tcatcttgca gggtaatcag
     2341 cccctgacgt tgcatctcat ttgccagcgc atcaataacg tccggcaact cgtcgcgatc
     2401 ccagcgcagg aacagctccg ctttcagcat tgggtaaagc acattgacgt gctccatcaa
     2461 tacgtcgcgg gagatgtggc gatgctgggt gacaattgcc gccatcagcg aaggcagcac
     2521 caacatatgc gcaatgttgt tgcgatagta ggtcatcagc accgcttgct cgcgcggcag
     2581 aatgatgatg tcgccgatag tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg
     2641 atcgataagc tcgctggcgc tggctgaagg aacggtagag tccgtggagt aaggtacgtt
     2701 gcgcatcaga tccaggtagc agttgagttg ctcggttaac tgctcgcggg tgagtgagcg
     2761 ctgacgtgat gccagaagcg cagtacaaca caggttcatg gcgtttgccg cgcctgcgtt
     2821 gttaatgcgt accatcagat cggcagcaat attattgacc gtcggcgtta accaggccgg
     2881 acgcaccgct tcgatgggat cgatagattc acgccagtct ggtacgtgct ggttaaggta
     2941 ggtcatcaac ggcattggtt caccgaagtt gacgtaaccc tgaccgagat tacgcagctt
     3001 gcttaaaccg cgcagcatct gcggcaggct ctctttctct ttcgtcgcgc cgcgcagttc
     3061 tttggcgtaa gtacccactt ccatgacgtg ctcataaccg atatagatcg gaatcagcgt
     3121 aatcggacgc gtgccgccac gcagcatcgc ctgaatggtc atcgacagcg taccggtttt
     3181 cggatccagc aaacgccccg tgcgggaacg accgccttcc acgaagtact cgacggaata
     3241 accacggctg aacagttcgc cgagatactc acggaaaacg gtggaataaa gtttattgcc
     3301 tttaaaagta cggcgaataa agaacgcccc cagacggcgg aaaatcggcc cggccggcca
     3361 gaagttcagg ttgatcccgg cggcgatatg cggcggcacc agcccctggt ggtacagcac
     3421 gtaagaaagt agcaggtagt ccatgtgact gcggtggcaa ggcacataca ccagctcatg
     3481 accgtcgtgg gccagctggc gaacgcgctc ggcgttatgg acgttgatgc cctgataaag
     3541 tcggttccag gtgaagccca gaatgcggtc agtcaggcgg atcatctcgt aagagaaatt
     3601 cgccgcaatc tcttccatca gcgcaatcgc gttctgctgc gctttttcat gggagatttt
     3661 tttgctgcgc gcttcatctt ctaccgcttt ggcaatggcg cgggaggcga gcagcttatt
     3721 aaacagatcc tgacgagcag gcagacgtgg gcctacggca gccagacgtt gacgggcaaa
     3781 gtgcatacgc gccacgcgcg ccagtttctg tgcgatagtt ttatccgtgc cgtgttcatc
     3841 cgccatacgg cgcagcgaaa ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca
     3901 cagtacggcg aaaaatttct gtacgccgtt aagcatacgc agcggcgggt ttacttcgcc
     3961 tttttcacgc cccggcgcgc gaccaaacat caccgatact ggcaccatct gcacatccag
     4021 atttgggttg ctacggtgca aatcgagata gtcgtggaac agcttaatag actcttcttt
     4081 cggcgtgtaa taggtgaata cacgcggccc gccgtgaatg aacacatagc gcggcagtag
     4141 cgtgccgtcg atttctagcg gctctaacgg gtcaggcaag tcatgagcca gacactgggc
     4201 gcgtaacgtc agcaaatctg ctttcgagtt gtacggtaaa acgtacataa ttggacgaga
     4261 ggtatccagc cccagttccg gggcaggatc tgccggaata gacttgcttt ttaccaggat
     4321 gcttaatggt aaattcagta atttgtagta aattcgtggc cagccggaca taaacgatgt
     4381 aaagcctctg gttaataatg caaatgcgcg gcaaggatag cagaaagtca tgggaaattc
     4441 tgtggtatcc gctcatgttt cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg
     4501 tacactgata ttgacgctca taatgtaaaa aggttctttc aatggccaat aataccactg
     4561 gattcacccg aattatcaaa gctgctggct attcctggaa aggtttacgc gctgcatgga
     4621 tcaacgaagc ggcattccgt caggaaggcg tagcggtatt gttggcggtg gtcatcgcct
     4681 gctggctgga tgtggacgca attacccgcg tgctgcttat cagctccgtg atgctggtga
     4741 tgattgtgga aattctcaat agcgccatcg aagcagtggt tgaccgaatt ggctctgaat
     4801 accatgagct ttccggacgc gctaaagata tgggatccgc cgcggtgctg attgcgatta
     4861 tcgtcgccgt gataacctgg tgcatcctgt tatggtcgca ttttggataa cccttccaga
     4921 tttcgataaa tctctggttt attgtgcagt ttatggttcc aaaatcgcct tttgctgtat
     4981 atactcacag cataactgta tatacaccca gggggcggaa tgaaagcgtt aacggccagg
     5041 caacaagagg tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg
     5101 cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg
     5161 aaggcgctgg cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt
     5221 ctgttgcagg aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg
     5281 cttctggcgc agcagcatat tgaaggtcat tatcaggtcg atccttcctt gttcaagccg
     5341 aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga aagatatcgg cattatggat
     5401 ggtgacttgc tggcagtgca taaaactcag gatgtacgta acggtcaggt cgttgtcgca
     5461 cgtattgatg acgaagttac cgttaagcgc ctgaaaaaac agggtaataa agtcgaactg
     5521 ttgccagaaa atagcgagtt taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc
     5581 attgaagggc tggcggttgg ggttattcgc aacggcgact ggctgtaaca tatctctgag
     5641 accgcgatgc cgccaggcgt cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc
     5701 atggcattcc tcacttcatc tgataaagca ctctggcatc tcgccttacc catgattttc
     5761 tccaatatca ccgttccgtt gctgggactg gtcgatacgg cggtaattgg tcatcttgat
     5821 agcccggttt atttgggcgg cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg
     5881 ctgttgctgt ttttacgcat gagcaccacc gggctgactg cgcaggctta tggtgccaaa
     5941 aatcctcagg cattagcccg tgcgctggtg caaccgttgc tgttggcgtt gggggctggg
     6001 gcgttaattg cgctgctgcg tacaccgatt atcgatctgg ctctgcatat tgttggcggt
     6061 agtgaagcag tcctggaaca ggcgcggcgc tttcttgaaa tccgctggtt aagcgcaccg
     6121 gcgtcgctgg cgaatctggt attactcggt tggttactcg gcgtgcaata tgcccgtgcg
     6181 ccagtaattt tgttagtggt cggcaatatc ctcaacattg tgctggatgt ctggctagtg
     6241 atggggctgc atatgaacgt gcagggcgcg gctctggcga cggttattgc ggaatatgca
     6301 acattgctga ttggtctgct aatggtgcgt aaaatcctca aactacgcgg aatttccggc
     6361 gaaatgtcga aaactgcctg gcgaggaaac ttccgtcgct tgctggcgct taaccgcgat
     6421 atcatgctgc gttcgctgtt gttgcaactc tgtttcggcg cgatcaccgt acttggcgcg
     6481 cgactgggga gtgacattat cgctgtaaac gcggttctga tgacgctact cacctttacc
     6541 gcctatgcgc tggatggttt tgcctacgcg gttgaagcac attccggtca ggcatacggt
     6601 gcgcgcgacg gtagccagtt gctggatgtc tggcgggcag cgtgccgcca gtcggggatc
     6661 gtagcgttac tattttcggt ggtttatttg ctggcagggg aacacatcat tgcgttgctg
     6721 acgtcgttaa cccagattca gcagctggct gaccgctatc ttatctggca ggtgattttg
     6781 ccgttggttg gcgtctggtg ttatctgctg gacggcatgt ttataggcgc aacgcgtgcc
     6841 gccgaaatgc gtaacagtat ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg
     6901 ctgccgtggc tgggtaatca tggtttgtgg ctggcattaa ccgtctttct ggcgttgcgc
     6961 gggctttctc tggcggctat ctggcggcgt cactggcgta acggtacctg gtttgccgca
     7021 acgtgacggt taaaaattct gaataaatta tcctaagcca aattgctgac tacacttaat
     7081 ctcacgttca gaagaaaagt gaacgtactc tcattcacaa cctaacgatg aggtcttgat
     7141 tatgaataaa gatgaagccg gcggtaactg gaaacagttt aaaggtaaag tgaaagagca
     7201 atggggcaaa ctgaccgatg atgatatgac gatcattgaa ggtaaacgtg atcaactggt
     7261 cggtaaaatc caggaacgtt atggttatca gaaagatcag gcagaaaaag aggtcgtgga
     7321 ttgggaaacc cgcaatgaat atcgctggta attaatccct cctgcccgac gtgtacaagg
     7381 atgtacgccc tcttaacgcg gtttcttttt cacctgcaca gagtgatcat gctggcactg
     7441 ttcaggatga cgacacgctt ccacttccac gcatgccgca cagagcccat gtgcttcaat
     7501 cacattatgc cgcagggcaa accccatttt tgccgccagc gtatgcataa tgtcttccac
     7561 gccttctgca cactcttctt tcaccgcgcc gcagcgatcg catataaaca tggctgacgt
     7621 atgcgtgggc tggtcgaaca gatgacagag cacataactg ttggtggatt ccaccttatg
     7681 cacaaaacct tgctcaagca gaaaatccag cgcgcgataa accgttggcg gcttggcttg
     7741 cggttcagct tcgcgcagta aatcaagcag atcataagcg ctgatagcgc catcctgcag
     7801 actcatcagg cgcaacactt ccaggcgctg tggggtcagg cgcacattac gctgcgcgca
     7861 gattttttca gcctgcgcta ataactcctg cgttgtggtc ttttccatcg ggcacctcaa
     7921 agtgggggtt aagaatgtcc ttactttacc atgttccagg aaaaacaccg agaaccctta
     7981 ttgttgccgt aatgttgatt ttctgttttg taggtaaggt gttatgttgc cttgtcgta
//
LOCUS       DAZRUF010000185         7978 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZRUF010000185 DAZRUF010000000
VERSION     DAZRUF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.186"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..499
                     /gene="radC"
                     /locus_tag="ACHXOL_003261"
     CDS             <1..499
                     /gene="radC"
                     /locus_tag="ACHXOL_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR6810172.1"
                     /translation="LLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPL
                     LSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREA
                     IKINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFA
                     ERGWI"
     gene            716..952
                     /gene="rpmB"
                     /locus_tag="ACHXOL_003262"
     CDS             716..952
                     /gene="rpmB"
                     /locus_tag="ACHXOL_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR6810173.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            973..1140
                     /gene="rpmG"
                     /locus_tag="ACHXOL_003263"
     CDS             973..1140
                     /gene="rpmG"
                     /locus_tag="ACHXOL_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR6810174.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1238..2047
                     /gene="mutM"
                     /locus_tag="ACHXOL_003264"
     CDS             1238..2047
                     /gene="mutM"
                     /locus_tag="ACHXOL_003264"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR6810175.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2086..2565)
                     /gene="coaD"
                     /locus_tag="ACHXOL_003265"
     CDS             complement(2086..2565)
                     /gene="coaD"
                     /locus_tag="ACHXOL_003265"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR6810176.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2573..3850)
                     /gene="waaA"
                     /locus_tag="ACHXOL_003266"
     CDS             complement(2573..3850)
                     /gene="waaA"
                     /locus_tag="ACHXOL_003266"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR6810177.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4263..5321
                     /gene="rfaQ"
                     /locus_tag="ACHXOL_003267"
     CDS             4263..5321
                     /gene="rfaQ"
                     /locus_tag="ACHXOL_003267"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR6810178.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5318..6442
                     /locus_tag="ACHXOL_003268"
     CDS             5318..6442
                     /locus_tag="ACHXOL_003268"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR6810179.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6435..7232
                     /gene="rfaP"
                     /locus_tag="ACHXOL_003269"
     CDS             6435..7232
                     /gene="rfaP"
                     /locus_tag="ACHXOL_003269"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR6810180.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7248..>7978
                     /locus_tag="ACHXOL_003270"
     CDS             7248..>7978
                     /locus_tag="ACHXOL_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR6810181.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQF"
BASE COUNT         2033 a         1842 c         2035 g         2068 t
ORIGIN      
        1 cttgttaacc tctgaatatg aacaatttag tggcgttcat ggaattggcg tagcgaaatt
       61 tgcccagtta aaggggattg ctgaactggc gcggcgttac tacaacgtgc ggatgcgtga
      121 agaaagccct ttactcagcc cggagatgac gcgggaattt ttacaaagcc agctcacggg
      181 tgaggagcgg gagatcttta tggtgatctt tctcgactca caacaccggg ttataacgca
      241 tagccgtctt ttttccggca cgctaaacca tgttgaagtc catcctcggg aaattatccg
      301 ggaagcgata aaaataaacg cctcggcgct gatccttgca cataatcacc cttcgggttg
      361 tgctgaaccc agtaaagcgg ataaactcat tactgaacgg ataataaaga gttgtcagtt
      421 catggattta cgcgtgctcg accatatcgt gattgggcgt ggagagtatg tttcttttgc
      481 cgaacgcggc tggatttaac ccgctatgcg cgatccttcg ggatctttgt ctgttcggga
      541 cttgagcaca tcgctgagtc agcgtatact acgccacctt tgagaatctc gggtttggca
      601 tttgggcctg gcaatcgaga gttcacatag aactgcgatg accgggctgt aaagcctgac
      661 gaggcgccaa taccccatac gaagctcgag ctaatttgat ttttggagaa tagacatgtc
      721 ccgagtctgc caagttactg gcaagcgtcc ggtgaccggt aacaaccgtt cccacgcact
      781 gaacgcgact aaacgccgtt tcctgccgaa cctgcactct caccgtttct gggttgagag
      841 cgaaaagcgt tttgtcaccc tgcgcgtatc tgctaaaggt atgcgtgtaa tcgataaaaa
      901 aggcatcgat acagttctgg ctgaactgcg tgcccgtggc gaaaagtact aagtacttag
      961 aggaaataaa tcatggctaa aggtattcgt gagaaaatca agctggtttc ttctgctggt
     1021 actggtcact tctataccac tacgaagaac aaacgtacta agccggaaaa actggaactg
     1081 aaaaaattcg atccagttgt tcgccagcac gtgatctaca aagaagcgaa aatcaaataa
     1141 ttctcgcttt gatgtaacaa aaaacctcgc tccggcgggg ttttttgtta tctgcttgcc
     1201 cccatattga ttgcatctgt tcattcctgg agatgctatg cctgaattac ccgaagttga
     1261 aaccagccgt cgcggcatag aaccgcatct cgttggtgca accattcttc atgcggtggt
     1321 gcgcaacgga cgcttgcgct ggccggtttc agaagagatc taccgtttaa gcgaccaacc
     1381 agtgcttagc gtgcagcggc gggctaaata tctgctgctg gagctgcctg agggctggat
     1441 tatcattcat ttggggatgt ctggcagcct gcgcatcctt ccagaagaac ttccccctga
     1501 aaagcatgac catgtggatt tggtgatgag caacggcaaa gtgctgcgct acaccgatcc
     1561 gcgccgcttt ggtgcctggc tgtggaccaa agagctggaa gggcacaacg tgctggctca
     1621 tcttggaccg gagccgctta gcgacgattt caatggtgag tatctgcatc agaagtgcgc
     1681 gaagaaaaaa acggcgatta aaccgtggct gatggataac aagctggtgg taggggtagg
     1741 gaatatctat gccagcgaat cactgtttgc ggcggggatc catccggatc ggctggcgtc
     1801 atcactgtcg ctggcagagt gtgaattgtt agctcgggtg attaaagcgg tgttgctgcg
     1861 ttcgattgag cagggtggta caacgctgaa agattttctg caaagtgatg gtaaaccggg
     1921 ctatttcgcg caggaattgc aggtttacgg gcgaaaaggt gagccgtgtc gggtgtgcgg
     1981 tacgccgatt gtggcgacta aacatgcgca gcgggcaacg ttttattgtc ggcagtgcca
     2041 gaagtaattc atacgcgccg gatggcatac catccggcat aaacgctacg ctaacttcgc
     2101 caccagcgcc tgatggacat tctccggcag gaaatgggtg acatcgccct gatggcgcgc
     2161 cacctctttc accaacgatg aagagataaa cgaccactct ttcgacggca tcagaaacac
     2221 actttccagt tccggcatta agtggcgatt catatgcgcc agctgcattt catattcaaa
     2281 atccgccacc gcacgcaggc cacgaatcag caccgtagcg tgttgattac gggcgaagtt
     2341 cgccatcaaa tcactaaacc caaccacttc cacgttcccc agatgcgcgg ttgcctgctg
     2401 tgtcagcgcc acacgctctt ccagggtaaa catcggtttt ttactggggc tggcggcaat
     2461 cgccagaata acgtgatcga acatttgcgt ggcgcgcgtc acgatatcga tatgaccatt
     2521 ggtaatggga tcgaaagtac ccggataaat cgcccgtttt tgcataacaa cctcaatgcg
     2581 ttttcggtgg caggtaaggt tccagcagtt gaagcagacg ctgtagcgcg ccctggtttt
     2641 gatacagtac ttcaacggca tgacggccat agaaactacg gtaatcggcg tcggtgagta
     2701 aagaggaaac ctcttttgca agcgtagtgg catcggtaac ggtaatcagc ccgcttgcct
     2761 gctccagccg cgcgcaaatg tctttgaaat taaaagtatg cggccccatc aataccggaa
     2821 tagcgtgtgc ggcagcttcc agaggattat gccctccacg ttcaaccagt gaaccgccaa
     2881 caaaggcgag atcggcaatg ccgtacagta acatcaactc gcccatcgta tcgccaacca
     2941 caacctgcgt gctggtggag gggacttccc ctgaagagcg tgtgatatag cttagtccag
     3001 cctggcggac aaggttaatc gcatccggga agcgttctgg atgacggggt accaggatga
     3061 gcaataaatt cgggaattgc tgtaataatg cctgatgtgc ggcgatcacc acactctctt
     3121 caccttcgtg agtgctggtg gcaatccata ccgggcggtg tggtgcccac tggcggcgca
     3181 gcgtcacggc tttagcagcc aactgcggcg ttacagaaat atcgaatttc aggctaccgg
     3241 taacggttac ctggttattt tttgcgccca gcgccacaaa acgtgcacca tcttcttcat
     3301 tttgcgcagc aatcagcgta atacgacgca gtaagcggcg gacgaattta cccagtttgg
     3361 cataacctgc ggccgagcgg gcagagagtc gcgcgttagc gatcaccagc ggaattttac
     3421 gtttatgtag cgccgcaatc aggttaggcc atagttcggt ttccataatc aacaccagtt
     3481 tggggtcgac tttattcagg aaacggttga gcgcatcggg caggtcatac ggcagataaa
     3541 cgtgctgaac atccttcccg aaagccgatt gtacgcgctc cgaaccggtt ggcgtcatgg
     3601 ttgtaacggt aatcggtaaa tcaggataac gatggcgcag cgcgcgcacc aacgggatcg
     3661 ccgccagagt ttcaccgacg gagacggagt gcagcataat gccgcctggt tttagcggat
     3721 ggcggtaaaa accgtaacgt tcaccccagc gttttcgata ggccggagcc ttacgtccgc
     3781 gcacccagag ccgtatccag atcagcggct gaataaggta gagaagggcg gtgtaaagca
     3841 attcgagcat agtaaatagc tgacttatgg atgtgctggg gattctatgt atttagctgt
     3901 ggctttacca ttacttttcc cgtttttgac ttaaatagca atttacccgc caattcttgc
     3961 tgaacatcta tacttttttt gtatttttat gcgattcatt gatttctgct gtacgtgcag
     4021 gaaggaatcg cagagcaaca gtgaaagaat atggttgcta atgattttat ttgattgatt
     4081 tctttattga aaatcaacaa tgtgcttaaa aagcattcgg gtcggattgc cgcgaaagaa
     4141 gtgatacact agcgcgtcaa tatcagtgcg gtatcagtat tcaggtagct gttgagcctg
     4201 gggcggtagc gtgctttttc tgcttcacct taatcggata atctcaacaa aaagagttac
     4261 ttgtggataa gccatttcga agaattttgc tcattaagat gcgttttcat ggggatatgt
     4321 tattaactac tcccgtcatt agttcgctga aaaaaaatta ccctgacgca aaaatcgatg
     4381 tgctgcttta tcaggacacc accccgatcc tgtctgaaaa tccagagatt aacgcgctct
     4441 acggcataaa aaataaaaaa gcaaaagcct cagaaaaaat tgccaacttt tttcatctca
     4501 tcaaggtatt acgtgccaat aagtatgacc ttatcgtcaa tcttaccgat caatggatgg
     4561 ttgctatact ggttcgctta ttaaatgccc gtgtgaaaat ttcccaggat tatcatcatc
     4621 ggcagtctgc tttttggcgt aaaagtttca cccatttagt gccgttgcag ggtggaaatg
     4681 tggtggaaag taacttatcc gtgctgacac cattgggact tgattcgttg gtgaagcaga
     4741 caaccatgag ttacccgcct gcaagctgga aacgtatgcg tcgcgaactt gatcacgctg
     4801 gtgttggaca aaattatgtg gttatccaac ctacggcgcg gcaaatcttc aaatgctggg
     4861 acaacgccaa gttttccgct gtgattgatg ccttacatgc tcgtggttat gaagtcgttc
     4921 tgacgtccgg cccagataaa gacgatctgg cctgcgtcaa tgaaattgcg cagggatgcc
     4981 agacgccacc agtaacggcg ctggctggaa aggtgacctt cccggaactt ggtgcgttaa
     5041 tcgatcatgc gcagctgttt attggcgttg attccgcacc ggcgcatatt gccgctgcag
     5101 ttaatacgcc gctgatatcg ctgtttggtg cgacagacca tattttctgg cgtccttggt
     5161 caaataacat gattcaattc tgggcgggag attaccggga aatgccaacg cgcgatcagc
     5221 gtgaccgaaa tgagatgtat ctttcggtta ttccggcggc agatgtcatt gctgctgtcg
     5281 ataaattact gccctcctcc acgacaggta cgtcgttatg atcgttgctt tttgtttata
     5341 taaatatttt ccctttggcg gtttgcagcg cgattttatg cgtattgctc agacagtcgc
     5401 cgcccgaggt catcatgttc gggtttatac ccagtcgtgg gaaggcgaat gccctgatgt
     5461 atttgaactg atcaaagtgc cggttaaatc gcataccaat cacgggcgca atgcggagta
     5521 ttttgcctgg gtgcaaaaac atttacgcga acatcccgtc gataaagtcg ttggattcaa
     5581 caaaatgccg gggctggacg tttattatgc cgctgatgtt tgttatgccg agaaagtcgc
     5641 gcaggaaaaa ggctttttct atcacctgac gtcacgttat cgccattatg ccgcctttga
     5701 gcgggcaacc ttcgaacagg gcaagccgac acagctgctg atgctgacag ataagcaaat
     5761 cgccgatttc cagaaacatt atcagactga agcggagcgt tttcatattc tgccaccggg
     5821 gatttatcct gatcgtaaat atagccagca gccagctaat agccgtgaaa tcttccgtaa
     5881 gaagaatgga ataaccgaac aacaatattt attgttgcag gtcggttcag acttcacgcg
     5941 taaaggtgtc gatcgttcca ttgaagcact tgcttcgtta ccggattcgc tgcgccacaa
     6001 cacattgcta tatgttgttg gacaggataa gccgcgaaaa tttgaggcgc tggcagaaaa
     6061 acgcggcgtg cgcagtaatg ttcacttctt ctcggggcgc aacgatgtct cggaattaat
     6121 ggcggcggcg gatttattac tgcatcctgc ctaccaggaa gcagcgggaa ttgtgctact
     6181 ggaagcgatt actgcaggat taccggtact aacaacagcc gtttgtggct atgcgcatta
     6241 tattgtcgac gccaattgcg gcgaggctat tgctgagcca ttccgccagg aaacattgaa
     6301 tgagatttta cgcaaagcgt taacgcaatc ttcattgcgc caggcctggg cggaaaatgc
     6361 gcgacattat gctgatacac aagatttata cagtctgcca gagaaagcgg cggatatcat
     6421 aacgggtggt ctggatggtt gaacttaaag agccgtttgc cacgttatgg cgcggtaaag
     6481 atccttttga ggaagttaaa accttgcagg gtgaggtttt tcgtgaactg gaaactcgcc
     6541 gcactctgcg atttgaaatg gcgggcaaaa gctattttct caaatggcat cgcggcacga
     6601 ccctgaaaga gataatcaaa aatttactct cattgcggat gccagtatta ggcgcagacc
     6661 gcgaatggaa tgcgattcat cgactgcggg atgtcggcgt tgatactatg tatggggtgg
     6721 catttggcga aaaaggtatt aatccgctga gcagaacctc gtttattatt accgaagatc
     6781 tgaccccaac catcagtctg gaagattact gtgctgactg ggcgaccaac ccaccagatg
     6841 ttcgcgtaaa gcgtatgctt attaagcgtg tcgcgacgat ggtgcgcgat atgcatgctg
     6901 tgggcattaa ccaccgtgac tgttatatct gtcatttcct gctgcacttg cctttttccg
     6961 gtaaggaaga ggagttaaaa atttcggtaa ttgacctgca ccgggcgcag cttcgcacgc
     7021 gcgttccacg tcgttggcgc gataaagatc ttattgggct ttatttttct tcgatgaata
     7081 tcggcctgac tcagcgggat atctggcggt ttatgaaagt gtattttgtc gccccgctta
     7141 aagacattct caagcaggaa caaggactgc tgtcgcaagc agaagcaaaa gccacaaaaa
     7201 tcagggaaag aacgattcga aaatcgttgt aatttccgga gagaaatatg agtgcccact
     7261 attttaatcc acaagagatg atcaataaga caatcatctt cgatgaaagg ccagcggcgt
     7321 cagtggcatc atcattccat gttgcttatg gcattgataa aaactttctt tttggttgtg
     7381 gtgtttcaat cacgtcagtt ttgttacata acaacgacgt gagttttgtt ttccacgttt
     7441 ttattgatga tatccctgaa gccgatatcc agcgtttagc ccaattggcg aaaagctatc
     7501 gtacctgtat ccagatccat ctggtaaatt gtgaacggct taaggcatta ccgacgacca
     7561 aaaattggtc tattgccatg tatttccgtt ttgtaattgc agattacttt attgatcaac
     7621 aagataagat cctttacctg gatgctgata tcgcctgtca gggaaactta aagccgctga
     7681 taacaatgga tcttgccaat aacgttgctg ctgttgttac tgaacgcgat gctaactggt
     7741 ggtcgttacg gggtcaaagt ctgcagtgta atgaacttga aaagggttac tttaattcag
     7801 gtgtcctgtt aattaataca ctagcgtggg cgcaggagtc cgtttctgct aaagcgatgt
     7861 cgatgcttgc tgataaagcc gtcgtttccc gtttaaccta tatggatcaa gatatactta
     7921 atcttatcct gtcagggaaa gttaaattca ttgatgctaa atacaatact caatttag
//
LOCUS       DAZRUF010000186         7977 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZRUF010000186 DAZRUF010000000
VERSION     DAZRUF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.187"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(60..548)
                     /gene="dfrA3"
                     /locus_tag="ACHXOL_003271"
     CDS             complement(60..548)
                     /gene="dfrA3"
                     /locus_tag="ACHXOL_003271"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF000019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA3"
                     /protein_id="HFR6810182.1"
                     /translation="MLISLIAALAHNNLIGKDNLIPWHLPADLRHFKAVTLGKPVVMG
                     RRTFESIGRPLPGRRNVVVSRNPQWQAEGVEVAPSLDAALALLTDCEEAMIIGGGQLY
                     AEALPRADRLYLTYIDAQLNGDTHFPDYLSLGWQELERSTHPADDKNSYACEFVTLSR
                     QR"
     gene            complement(554..1021)
                     /locus_tag="ACHXOL_003272"
     CDS             complement(554..1021)
                     /locus_tag="ACHXOL_003272"
                     /inference="COORDINATES: protein motif:HMM:NF024230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFR6810183.1"
                     /translation="MKELVSLALWDAFWSAIPAVGFAMLFSVPPRFLKYCAVVGALAH
                     SMRAVLMHYGLPIEWATLAASSFVSLVFVYLSRRLLAPRPVFTVACIIPMIPGKFAFN
                     TIIAVLSMNSEGVSGHLINAAIENGLKTLFILMALSFGLAIPPLIIYRNRPIV"
     gene            complement(1018..1371)
                     /locus_tag="ACHXOL_003273"
                     /pseudo
     CDS             complement(1018..1371)
                     /locus_tag="ACHXOL_003273"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
     gene            complement(1827..2111)
                     /locus_tag="ACHXOL_003274"
     CDS             complement(1827..2111)
                     /locus_tag="ACHXOL_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="HFR6810184.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRR
                     KVLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     gene            2311..2952
                     /locus_tag="ACHXOL_003275"
                     /pseudo
     CDS             2311..2952
                     /locus_tag="ACHXOL_003275"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
     gene            3059..3472
                     /locus_tag="ACHXOL_003276"
     CDS             3059..3472
                     /locus_tag="ACHXOL_003276"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="HFR6810185.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQA
                     SEAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLA
                     SMMPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     gene            3571..4440
                     /locus_tag="ACHXOL_003277"
     CDS             3571..4440
                     /locus_tag="ACHXOL_003277"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="HFR6810186.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDD
                     LSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPA
                     SADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQ
                     RQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLAS
                     RGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPR
                     QRGMDRGGPDFSM"
     gene            4501..4713
                     /locus_tag="ACHXOL_003278"
     CDS             4501..4713
                     /locus_tag="ACHXOL_003278"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810187.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMA
                     SLNLDGCTLSLFREDKPFGPGKFLGD"
     gene            4715..4921
                     /gene="urfF"
                     /locus_tag="ACHXOL_003279"
     CDS             4715..4921
                     /gene="urfF"
                     /locus_tag="ACHXOL_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="HFR6810188.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDE
                     YEALRECLEELRAAQGGGSDPASA"
     gene            4951..5790
                     /locus_tag="ACHXOL_003280"
     CDS             4951..5790
                     /locus_tag="ACHXOL_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFR6810189.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKS
                     MLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAV
                     ADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVI
                     GRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSA
                     EAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPR
                     GEA"
     gene            5777..6628
                     /gene="repC"
                     /locus_tag="ACHXOL_003281"
     CDS             5777..6628
                     /gene="repC"
                     /locus_tag="ACHXOL_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="HFR6810190.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
                     YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
                     AVTADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
                     LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
                     DPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDI
                     TRPKAAG"
     gene            6936..7751
                     /gene="sul2"
                     /locus_tag="ACHXOL_003282"
     CDS             6936..7751
                     /gene="sul2"
                     /locus_tag="ACHXOL_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR6810191.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT         1691 a         2428 c         2435 g         1423 t
ORIGIN      
        1 aaaaaaaacc gccctgtttt ttggcggttt ttttgtatct ggtggtgcca ataggcggtt
       61 cagcgctgac gactcaaggt aacaaattcg caggcatagc tgttcttatc gtcagcagga
      121 tgcgttgacc gctccaactc ctgccaccca agcgataggt aatccgggaa atgggtatca
      181 ccgttcaact gagcgtcaat gtaggttaga tacaagcgat ccgctcgggg cagagcctcg
      241 gcatagagtt gcccgccacc gatgatcatc gcttcctcac agtcggttaa tagcgccaga
      301 gccgcatcca gcgagggagc cacctccacc ccttcggcct gccattgggg attgcgacta
      361 acgacaacat tgcgccgtcc tggcaatggc cgcccgatcg actcaaaggt gcgacgtccc
      421 atcaccacag gtttccccag ggtgacagct ttgaaatgac gcagatcggc aggtagatgc
      481 catggaataa gattatcttt gccaatcaag ttgttatgag ccaaagctgc aatcaaagaa
      541 atcaacataa tcctcacaca atcggacggt tgcggtaaat aatcaacggg ggaatagcca
      601 ggccgaagct cagggccatc aaaataaaca gcgtcttcaa tccattctcg atagcagcat
      661 tgatgagatg cccactgacg ccttcgctgt tcatgctcag cacggcaatg atggtattga
      721 acgcgaattt gccgggtatc atagggatta tgcaggccac ggtaaagacc gggcgcggcg
      781 ccagcaaacg acgggacaga tagacaaaca ccaggctgac aaaactgctg gccgcaagcg
      841 tggcccactc gatcggcaac ccgtaatgca tcagcacggc gcgcatgcta tgcgccagag
      901 ccccgaccac ggcgcaatac ttcaagaaac gcggtggcac tgaaaacaac atggcgaagc
      961 caaccgctgg gatagccgac cagaatgcgt cccacaatgc cagactaacc agctctttca
     1021 tggctgccac ccccagactc cggtgagcat catcgccaaa acaataccgg ttgtggcact
     1081 cagggtcagc atggttgcgg tcaaccagcg cgccaacccc atgttgtaat agcctttgac
     1141 cacatccagc atggcattga tcaacggaaa gccaggcacc agcagcaaaa cgcaggcagc
     1201 catcgccagc tggggttgcg ccccccagtt attcagggta gccaggctgg cgatcaaggt
     1261 cgcaacaaag gctgtcgcgg aaaagttcaa cagtggactg aaatggcgat gggcaatttc
     1321 ctgacgtaca aacataccga cggatgaagc cagaaccgtg atcaggaata ccggccagtg
     1381 cccgtcactc ccgtactaac tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa
     1441 taactgtcac gaacccctgc aataactgtc acgcccccaa acctgcaaac ccagcagggg
     1501 cgggggctgg cggggtgttg gaaaaatcca tccatgatta tctaagaata atccactagg
     1561 cgcggttatc agcgcccttg tggggcgctg ctgcccttgc ccaatatgcc cggccagagg
     1621 ccggatagct ggtctattcg ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg
     1681 gggaaaggcg ggcaaagccc gctaaacccc acaccaaacc ccgcagaaat acgctggagc
     1741 gcttttagcc gctttagcgg cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc
     1801 ccgcagggcc tgtctcggtc gatcattcag cccggctcat ccttctggcg tggcggcaga
     1861 ccgaacaagg cgcggtcgtg gtcgcgttca aggtacgcat ccattgccgc catgagccga
     1921 tcctccggcc actcgctgct gttcaccttg gccaaaatca tggcccccac cagcaccttg
     1981 cgccttgttt cgttcttgcg ctcttgctgc tgttcccttg cccgcacccg ctgaatttcg
     2041 gcattgattc gcgctcgttg ttcttcgagc ttggccagcc gatccgccgc cttgttgctc
     2101 cccttaacca tcttgacacc ccattgttaa tgtgctgtct cgtaggctat catggaggca
     2161 cagcggcggc aatcccgacc ctactttgta gggggagggc gcacttaccg gtttctcttc
     2221 gagaaactgg cctaacggcc acccttcggg cggtgcgctc tccgagggcc attgcatgga
     2281 gccgaaaagc aaaagcaaca gcgaggcagc atggcgattt atcaccttac ggcgaaaacc
     2341 ggcagcaggt cgggcggcca atcggccagg gccaaggccg actacatcca gcgcgaaggc
     2401 aagtatgccc gcgacatgga tgaagtcttg cacgccgaat ccgggcacat gccggagttc
     2461 gtcgagcggc ccgccgacta ctgggatgct gccgacctgt atgaacgcgc caatgggcgg
     2521 ctgttcaagg aggtcgaatt tgccctgccg gtcgagctga ccctcgacca gcagaaggcg
     2581 ctggcgtccg agttcgccca gcacctgact ggtgccgagc gcctgccgta tacgctggcc
     2641 atccatgccg gtggcggcga gaacccgcac tgccacctga tgatctccga gaggatcaat
     2701 gacggcatcg agcggcccgc cgctcagtgg ttcaagcggt acaacggcaa gaccccggag
     2761 aagggcgggg cacagaagac cgaagcgctc aagcccaagg catggcttga gcagacccgc
     2821 gaggcatggg ccgaccatgc caaccgggca ttagagcggg ctggccacga cgcccgcatt
     2881 gaccacagaa cacttgaggc gcagggcatc gagcgcctgc ccggtgttca cctggggccg
     2941 aacgtggtgg agatggaagg ccggggcatc cgcaccgacc gggcagacgt ggccctgaac
     3001 atcgacaccg ccaacgccca gatcatcgac ttacaggaat accgggaggc aatagaccat
     3061 gaacgcaatc gacagagtga agaaatccag aggcatcaac gagttagcgg agcagatcga
     3121 accgctggcc cagagcatgg cgacactggc cgacgaagcc cggcaggtca tgagccagac
     3181 ccagcaggcc agcgaggcgc aggcggcgga gtggctgaaa gcccagcgcc agacaggggc
     3241 ggcatgggtg gagctggcca aagagttgcg ggaggtagcc gccgaggtga gcagcgccgc
     3301 gcagagcgcc cggagcgcgt cgcgggggtg gcactggaag ctatggctaa ccgtgatgct
     3361 ggcttccatg atgcctacgg tggtgctgct gatcgcatcg ttgctcttgc tcgacctgac
     3421 gccactgaca accgaggacg gctcgatctg gctgcgcttg gtggcccgat gaagaacgac
     3481 aggactttgc aggccatagg ccgacagctc aaggccatgg gctgtgagcg cttcgatatc
     3541 ggcgtcaggg acgccaccac cggccagatg atgaaccggg aatggtcagc cgccgaagtg
     3601 ctccagaaca cgccatggct caagcggatg aatgcccagg gcaatgacgt gtatatcagg
     3661 cccgccgagc aggagcggca tggtctggtg ctggtggacg acctcagcga gtttgacctg
     3721 gatgacatga aagccgaggg ccgggagcct gccctggtag tggaaaccag cccgaagaac
     3781 tatcaggcat gggtcaaggt ggccgacgcc gcaggcggtg aacttcgggg gcagattgcc
     3841 cggacgctgg ccagcgagta cgacgccgac ccggccagcg ccgacagccg ccactatggc
     3901 cgcttggcgg gcttcaccaa ccgcaaggac aagcacacca cccgcgccgg ttatcagccg
     3961 tgggtgctgc tgcgtgaatc caagggcaag accgccaccg ctggcccggc gctggtgcag
     4021 caggctggcc agcagatcga gcaggcccag cggcagcagg agaaggcccg caggctggcc
     4081 agcctcgaac tgcccgagcg gcagcttagc cgccaccggc gcacggcgct ggacgagtac
     4141 cgcagcgaga tggccgggct ggtcaagcgc ttcggtgatg acctcagcaa gtgcgacttt
     4201 atcgccgcgc agaagctggc cagccggggc cgcagtgccg aggaaatcgg caaggccatg
     4261 gccgaggcca gcccagcgct ggcagagcgc aagcccggcc acgaagcgga ttacatcgag
     4321 cgcaccgtca gcaaggtcat gggtctgccc agcgtccagc ttgcgcgggc cgagctggca
     4381 cgggcaccgg caccccgcca gcgaggcatg gacaggggcg ggccagattt cagcatgtag
     4441 tgctttcgtt ggtactcacg cctgttatac tatgagtact cacgcacaga agggggtttt
     4501 atggaatacg aaaaaagcgc ttcagggtcg gtctacctga tcaaaagtga caagggctat
     4561 tggttgcccg gtggctttgg ttatacgtca aacaaggccg aggctggccg cttttcagtc
     4621 gctgatatgg ccagccttaa ccttgacggc tgcaccttgt ccttgttccg cgaagacaag
     4681 cctttcggcc ccggcaagtt tctcggtgac tgatatgaaa gaccaaaagg acaagcagac
     4741 cggcgacctg ctggccagcc ctgacgctgt acgccaagcg cgatatgccg agcgcatgaa
     4801 ggccaaaggg atgcgtcagc gcaagttctg gctgaccgac gacgaatacg aggcgctgcg
     4861 cgagtgcctg gaagaactca gagcggcgca gggcgggggt agtgaccccg ccagcgccta
     4921 accaccaact gcctgcaaag gaggcaatca atggctaccc ataagcctat caatattctg
     4981 gaggcgttcg cagcagcgcc gccaccgctg gactacgttt tgcccaacat ggtggccggt
     5041 acggtcgggg cgctggtgtc gcccggtggt gccggtaaat ccatgctggc cctgcaactg
     5101 gccgcacaga ttgcaggcgg gccggatctg ctggaggtgg gcgaactgcc caccggcccg
     5161 gtgatctacc tgcccgccga agacccgccc accgccattc atcaccgcct gcacgccctt
     5221 ggggcgcacc tcagcgccga ggaacggcaa gccgtggctg acggcctgct gatccagccg
     5281 ctgatcggca gcctgcccaa catcatggcc ccggagtggt tcgacggcct caagcgcgcc
     5341 gccgagggcc gccgcctgat ggtgctggac acgctgcgcc ggttccacat cgaggaagaa
     5401 aacgccagcg gccccatggc ccaggtcatc ggtcgcatgg aggccatcgc cgccgatacc
     5461 gggtgctcta tcgtgttcct gcaccatgcc agcaagggcg cggccatgat gggcgcaggc
     5521 gaccagcagc aggccagccg gggcagctcg gtactggtcg ataacatccg ctggcagtcc
     5581 tacctgtcga gcatgaccag cgccgaggcc gaggaatggg gtgtggacga cgaccagcgc
     5641 cggttcttcg tccgcttcgg tgtgagcaag gccaactatg gcgcaccgtt cgctgatcgg
     5701 tggttcaggc ggcatgacgg cggggtgctc aagcccgccg tgctggagag gcagcgcaag
     5761 agcaaggggg tgccccgtgg tgaagcctaa gaacaagcac agcctcagcc acgtccggca
     5821 cgacccggcg cactgtctgg cccccggcct gttccgtgcc ctcaagcggg gcgagcgcaa
     5881 gcgcagcaag ctggacgtga cgtatgacta cggcgacggc aagcggatcg agttcagcgg
     5941 cccggagccg ctgggcgctg atgatctgcg catcctgcaa gggctggtgg ccatggctgg
     6001 gcctaatggc ctagtgcttg gcccggaacc caagaccgaa ggcggacggc agctccggct
     6061 gttcctggaa cccaagtggg aggccgtcac cgctgatgcc atggtggtca aaggtagcta
     6121 tcgggcgctg gcaaaggaaa tcggggcaga ggtcgatagt ggtggggcgc tcaagcacat
     6181 acaggactgc atcgagcgcc tttggaaggt atccatcatc gcccagaatg gccgcaagcg
     6241 gcaggggttt cggctgctgt cggagtacgc cagcgacgag gcggacgggc gcctgtacgt
     6301 ggccctgaac cccttgatcg cgcaggccgt catgggtggc ggccagcatg tgcgcatcag
     6361 catggacgag gtgcgggcgc tggacagcga aaccgcccgc ctgctgcacc agcggctgtg
     6421 tggctggatc gaccccggca aaaccggcaa ggcttccata gataccttgt gcggctatgt
     6481 ctggccgtca gaggccagtg gttcgaccat gcgcaagcgc cgccagcggg tgcgcgaggc
     6541 gttgccggag ctggtcgcgc tgggctggac ggtaaccgag ttcgcggcgg gcaagtacga
     6601 catcacccgg cccaaggcgg caggctgacc ccccccactc tattgtaaac aagacatttt
     6661 tatcttttat attcaatggc ttattttcct gctaattggt aataccatga aaaataccat
     6721 gctcagaaaa ggcttaacaa tattttgaaa aattgcctac tgagcgctgc cgcacagctc
     6781 cataggccgc tttcctggct ttgcttccag atgtatgctc ttctgctcct gcagctaatg
     6841 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgat
     6901 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     6961 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     7021 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     7081 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     7141 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     7201 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     7261 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     7321 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     7381 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     7441 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     7501 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     7561 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     7621 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     7681 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     7741 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     7801 aggaacctcc attgaatcga actaatattt tttttggtgc ctcgcattca tgccctggat
     7861 acgggttgtg tctggggtaa tcagctcacg ctctggcgat gggatgatga tgccatcttc
     7921 agcctgggtt gccccatgta cgccaaaggt gactaatccg ctcaacagag cgccaat
//
LOCUS       DAZRUF010000187         7957 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZRUF010000187 DAZRUF010000000
VERSION     DAZRUF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.188"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(283..1314)
                     /gene="rsmC"
                     /locus_tag="ACHXOL_003283"
     CDS             complement(283..1314)
                     /gene="rsmC"
                     /locus_tag="ACHXOL_003283"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR6810192.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1417..1830
                     /gene="holD"
                     /locus_tag="ACHXOL_003284"
     CDS             1417..1830
                     /gene="holD"
                     /locus_tag="ACHXOL_003284"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR6810193.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1799..2245
                     /gene="rimI"
                     /locus_tag="ACHXOL_003285"
     CDS             1799..2245
                     /gene="rimI"
                     /locus_tag="ACHXOL_003285"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR6810194.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2260..2937
                     /gene="yjjG"
                     /locus_tag="ACHXOL_003286"
     CDS             2260..2937
                     /gene="yjjG"
                     /locus_tag="ACHXOL_003286"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR6810195.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3028..4617
                     /gene="prfC"
                     /locus_tag="ACHXOL_003287"
     CDS             3028..4617
                     /gene="prfC"
                     /locus_tag="ACHXOL_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR6810196.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5010..5615
                     /gene="osmY"
                     /locus_tag="ACHXOL_003288"
     CDS             5010..5615
                     /gene="osmY"
                     /locus_tag="ACHXOL_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR6810197.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5742..5903
                     /locus_tag="ACHXOL_003289"
     CDS             5742..5903
                     /locus_tag="ACHXOL_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR6810198.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6025..7098
                     /locus_tag="ACHXOL_003290"
     CDS             6025..7098
                     /locus_tag="ACHXOL_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR6810199.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7095..7877
                     /locus_tag="ACHXOL_003291"
     CDS             7095..7877
                     /locus_tag="ACHXOL_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR6810200.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1898 a         2055 c         2157 g         1847 t
ORIGIN      
        1 ttccgccacc ttcgcatacc atcaattctt aaaaagaatt gctaccacgg aggcgcattc
       61 tagtggtttt cagcttttcg tcaatagtta attatcgccg gaggtgtaat tgctggaaaa
      121 atgtccatca ggaaactagc gtgcaggttt ggtaggcatg cgggggcaga tgccagatgc
      181 gacgctggcg cgtcttatct ggcctacgaa gggctaacgt gcaggttttg taggtcggat
      241 aaggcgttca cgccgcatcc gacacggtat tcggcgagat aattaacctt tcttcgcctg
      301 gcgggtcata atggcgcgat acaccttgaa acgcccggtt tgcgcaatca cttcatggaa
      361 gccaaatgtc tcatccagca cgtccgggta aggcaggaag gcgttcgcta caattcgcag
      421 ctcgccgccg ctattaagat gacgcaccgc gccgcgaatc agcgtttgcg ccgcatccag
      481 gctggtttgc atcccatcgt ggaacggcgg gttggagatg atcatatcaa aacgaccttt
      541 cacctcggaa aagacgttgc tggcaaagac ttcaccttca acacagttgg ccgcaagtgt
      601 tgcgcggctg gcttctaccg ctggcgcaga gacatcgcac aaggtgagac gaattttcgg
      661 cgaatggcgc gcaaaggcaa ctgaaagcac ccccgcgcca cagccgacat ccagcacttt
      721 acctttcgtg tgcggagtta acgtcgagag cagcaactgg ctaccgacat ccagaccgtc
      781 gcggctaaac acgccaggca gcgttttgac cgtcaggcca tcgacgctgt attcgcccca
      841 gaatttatcg gcatcaaata ccggctgttt ttccagacga ccaaaataga ggccacagcg
      901 acgagcgctg tcgactttat tcaacggcgc ataatctgcc agcatctgct cggcgctgcg
      961 cacgccgctg cggttctcgc caacgacaaa aatatctgtc cccactggca gcagagaaag
     1021 taaattcatc aactggaact gggcttccgg tttgttcttc ggccagtagt aaatcagcgt
     1081 atcgcaatct gcgacgtcat ccaccgtggc gaccagactg aaacgggcgt tatcccccat
     1141 ctggcggctt aatacctgcc agtggtggaa ttgctgggta tgagcacggc tggccgcggt
     1201 atctaaacgc gcgggcaggt catcctgtaa gtctccggca aacagaatac ggctttgttc
     1261 gaaatcatca ctgtgacgca gcaagacttc acttgccggg gtaaatgcag acatggaatg
     1321 ctcctcaatt gatactggcg gcgattatag ccatatgttg gcgcggtatc gacgaatttg
     1381 ctatatttgc gcccctgaca acaggagcga ttcgctatga catcccgacg agactggcag
     1441 ttacagcaac tgggcattac ccagtggtcg ctgcgtcgcc ctggcgcgtt gcagggggag
     1501 attgccattg cgatcccggc acacgtccgt ctggtgatgg tggcaaacga tcttcccgcc
     1561 ctgaccgatc ctttagtgag cgatgttctg cgcgcattaa ccgtcagccc cgatcaagtg
     1621 ctgcaactga cgccagaaaa aatcgcgatg ctgccgcaag gcagtcgctg caacagctgg
     1681 cggttgggta ctgacgaacc gctatcactg gaaggcgctc aggtggcatc accggcgctc
     1741 accgaattac gggcaaaccc aacggcacgc gccgcgttat ggcaacagat ttgcacatat
     1801 gaacacgatt tcttccctcg aaacgactga tttaccagcg gcttaccaca ttgaacaacg
     1861 cgcccacgcc tttccgtgga gtgaaaaaac ctttgccagc aaccagggcg agcgttatct
     1921 caactttcag ttaacgcaaa acggcaaaat ggcggcgttt gcgattacgc aagtggtgct
     1981 ggatgaagct acattgttca atattgcggt cgatcctgac tatcagcgtc agggattggg
     2041 aagggtgctg ctggaacatc tgatcgacga actggaaaaa cgcggcgtgg cgacactatg
     2101 gctggaagtc cgtgcctcaa atgctgccgc cattgccctg tacgaaagtt taggctttaa
     2161 cgaggcgacg attcgccgca attactaccc caccacggac ggtcgcgaag acgccatcat
     2221 catggcgttg ccaatcagta tgtaagacaa ggtggaataa tgaagtggga ctggattttc
     2281 tttgatgccg atgaaacgct gtttaccttt gactcattca ccggcctgca gcggatgttt
     2341 cttgattaca gcgtcacctt taccgctgaa gattttcagg actatcaggc cgttaacaag
     2401 ccactgtggg tggattatca aaacggcgcg atcacttcat tacagcttca gcacgggcgt
     2461 tttgagagct gggccgaacg gctgaaagtt gaagcaggct tgcttaacga tgcctttatt
     2521 aatgcgatgg cggaaatctg cacgccgctg ccgggcgcgg tttctctgct taacgccatt
     2581 cgtggcaacg ccaaaatcgg catcatcacc aacggcttta gcgccttgca gcaagtgcgt
     2641 ctggaacgca cgggcctgcg tgattacttc gatttgctgg tgatttccga agaagttggc
     2701 gttgccaaac cgaataagaa aattttcgat tatgcgctgg aacaggcggg caatcctgac
     2761 cgttcacgcg tgctgatggt tggcgacact gccgagtccg atattctcgg tggcatcaac
     2821 gccgggcttg cgacctgctg gctgaatgcg cacaatcgcg agcaaccaga aggcatcgcg
     2881 cccacctgga ccgtttcttc gttgcacgaa ctggagcagc tcctgtgtaa acactgattg
     2941 cctccccccc gttgatgggt aaaatagccg caatttttcg ttttcaacaa gcgcggcgcg
     3001 atgccgctta ctcaagaaga aagaattatg acgttgtctc cttatttgca agaggtggcg
     3061 aagcgccgca cttttgccat tatttctcac ccggatgccg gtaagactac catcaccgag
     3121 aaggtgctgc tgttcggaca ggccattcag accgccggta cagtaaaagg ccgtggttcc
     3181 aaccagcacg ctaagtcgga ctggatggag atggaaaagc agcgtgggat ctccattact
     3241 acgtctgtga tgcagtttcc gtatcacgat tgcctggtta acctgctcga caccccgggg
     3301 cacgaagact tctcggaaga tacctatcgt accctgacgg cggtggactg ctgcctgatg
     3361 gttatcgacg ctgcaaaagg tgttgaagat cgtacccgta agctgatgga agttacccgt
     3421 ctgcgcgaca cgccgatcct cacctttatg aacaaacttg accgtgatat ccgcgacccg
     3481 atggaactgc tcgatgaagt tgagaacgag ctgaaaatcg gctgcgcacc gatcacctgg
     3541 ccgattggct gcggcaagct gtttaaaggc gtttaccacc tttataaaga cgaaacctat
     3601 ctctatcaga gcggtaaagg ccacacgatt caggaagtcc gtattgttaa agggctgaat
     3661 aacccggatc tcgacgctgc ggttggtgaa gatctggcac agcagctacg tgacgaactg
     3721 gaactggtga aaggcgcgtc taacgagttc gacaaagaat tgttccttgc gggcgaaatc
     3781 actccggtat tcttcggtac tgcgctgggt aacttcggcg ttgatcatat gctggatggc
     3841 ctggtggagt gggctcctgc gccgatgccg cgtcagactg atacccgtac cgtagaggcg
     3901 agcgaagaca aatttaccgg cttcgtattt aaaattcagg ccaacatgga cccgaaacac
     3961 cgcgaccgcg tggcgtttat gcgcgtagta tccggtaaat atgaaaaagg catgaagctg
     4021 cgccaggtgc gtactgcgaa agatgtggtt atctccgacg cgctgacctt tatggcgggc
     4081 gaccgttcgc acgttgaaga agcgtatccg ggcgatatcc tcggcctgca caaccacggc
     4141 actattcaga tcggcgacac ctttacccag ggtgagatga tgaagttcac cggtattccg
     4201 aacttcgcac cagaactgtt ccgtcgtatc cgcctgaaag atccgctgaa gcaaaaacag
     4261 ctgctcaaag ggctggtaca gctttccgaa gagggcgcgg tgcaggtgtt ccgtccgatc
     4321 tccaacaacg atttgatcgt tggtgctgtt ggtgtgctgc agtttgatgt ggtggtatcg
     4381 cgcctgaaga gcgaatacag cgttgaagca gtatatgaat cagtcaacgt tgccactgcc
     4441 cgctgggtag aatgtgcgga cgcgaagaaa ttcgaagagt tcaagcgtaa gaacgaaagc
     4501 caactggcgc ttgatggcgg cgataacctc gcttacatcg ctaccagcat ggtcaacctg
     4561 cgcctggcgc aggaacgtta tcccgacgtt cagttccacc agacccgcga gcattaattc
     4621 ttgcctccag ggcgcggtag ccgctgcgcc ctgtcaattt cccttcctta ttagccgctt
     4681 acggaatgtt cttaaaacat tcacttttgc ttatgttttc gctgatatcc cgagcggttt
     4741 caaaattgtg atctatattt aacaaagtaa tgacatttct gacggcgtta aataccgttc
     4801 aatgcgtaga tatcagtatc taaagccgtc gattgtcatt ctaccgatat taataactga
     4861 ttcagaggct gtaatggtcg ttattcatca cccatcgctt ttgtgatggc gaccattgac
     4921 ttctgtagag ggtgaagtct ctccctattc agcaatgcaa cctcgtgttg ccaggctcaa
     4981 attacgagca aacatacagg aataaatcga tgactatgac aagactgaag atttcgaaaa
     5041 ctctgctggc tgtaatgttg acctctgccg tcgcgaccgg ctctgcctac gcggaaaaca
     5101 acgcacagac taccaatgaa agcgcagggc aaaaagtcga tagctctatg aataaagtcg
     5161 gtaatttcat ggatgacagc gccatcaccg cgaaagtgaa ggcggcgctg gtggatcatg
     5221 acaacatcaa gagcaccgat atctctgtca aaaccgatca aaaagtcgtg accctgagcg
     5281 gtttcgttga aagccaggct caggccgaag aggcagtgaa agtggcgaaa ggcgttgaag
     5341 gggtgacctc tgtcagcgac aaactgcacg ttcgcgacgc taaagaaggc tctgtgaagg
     5401 gctacgcggg tgacaccgcc accaccagtg aaatcaaagc caaactgctg gcggacgata
     5461 tcgtcccttc ccgtcatgtg aaagttgaaa ccaccgacgg cgtggttcag ctctccggta
     5521 ccgtcgattc ccaggcacag agcgaccgtg ctgaaagtat cgccaaagcg gtagatggtg
     5581 tgaaaagcgt taaaaatgat ctgaaaacta agtaattcgt cgtaattcgt cctcccgaac
     5641 tttgtcggga ggcgcgatgt gcaccacact aaaaatatcg cattgagtga ttttcacgct
     5701 catatcaagc ggtgacatta actatggtaa aggagacgct tatgtttcgt tggggcatca
     5761 tatttctggt tatcgcgtta atcgccgccg cacttgggtt tggtggtctg gccggtaccg
     5821 ctgcaggcgc agctaaaatt gtctttgtcg tcgggattat tctgttcctg gtgagtttgt
     5881 tcatgggccg aaaacgaccc tagatttcag accattaaga aaattttatc caaagccagt
     5941 ccagcggact ggcttttgcg gttttagcga ataataaatt gcgttacttt gtcattcttt
     6001 cacaacaaaa acaggaaggc agaggtgggg cagcgaatac ctgtaacgct tggtaatatt
     6061 gcgccgttgt cgctaaggcc gttccagcct ggacgaatag ctctggtgtg cgaaggcggc
     6121 ggacagcgtg gaattttcac ggctggcgtg ctggatgagt ttatgcgcgc gcagtttaat
     6181 cctttcgatc tttatctcgg cacatctgcc ggggcgcaga acctctcggc gtttatctgc
     6241 aatcagcccg gttacgcgcg caaagtcatc atgcgctata ccacaaaacg cgaatttttc
     6301 gatccattgc gctttgtccg tggaggaaat cttatcgatc tcgactggct ggtggaggcc
     6361 accgcaagcc agatgccgtt gcaaatggat accgccgcgc ggttgtttga cagcggcaaa
     6421 tcgttttata tgtgcgcctg tcgtcaggat gactacgcgc ctaattactt tttaccaacc
     6481 aaacaaaact ggctggatgt gattcgcgcc tccagtgcga tacctggctt ttatcgtagc
     6541 ggagtgtcgc tggaaggcat taactacctg gatggcggga tcagtgatgc gattccggtt
     6601 aaagaggcgg caaggcaggg cgctaaaacg ttggtcgtca ttcgcactgt gccgtcacaa
     6661 atgtactaca cgccgcagtg gttcaaacgc atggaacgct ggctgggtga cagtagcctg
     6721 cagccgctgg tcaatctggt gcagcatcat gaaaccagct atcgtgacat tcagcaattt
     6781 attgagaaac caccgggcaa gctgcggata ttcgaaattt atccgccgaa gccattacat
     6841 agtatcgcgc ttggcagtcg gattccggcg ctgcgtgaag actataaact tgggcgttta
     6901 tgcggtcgtt atttcctcgc cacggttggc aagctattaa ctgaaaaagc gccgcttacc
     6961 cgccatctgg tgccagtggt gacgccggaa tcgatcgtta ttccgcctgc gccagtcgcc
     7021 aacgatacgc tggttgccga agtgagcgat gctccgcagg cgaacgaccc gacatttaac
     7081 aatgaggatc tggcttgatt tgccgtttta tcgataccca ctgccatttt gatttcccgc
     7141 cgtttagtgg cgatgaagag gccagcctgc aacgcgcggc acaagcgggc gtaggcaaga
     7201 tcattgttcc ggcgacagag gcggaaaatt ttgcccgcgt gcaggcgtta gcggaaaaat
     7261 atcaaccgct gtatgccgca ttggggttgc atccggggat gttggaaaaa catagcgatg
     7321 tgtctcttga tcagctacag caggcgctgg aaaggcgtcc ggcgaaggtg gtggcggtgg
     7381 gggagatcgg tctggatctc ttcggcgacg atccgcaatt tgagaggcag cagtggttac
     7441 tcgacgaaca gctgaaactg gcgaaacgct acgatctgcc ggtgatcctt cattcccggc
     7501 gtatacacga caaactggcg atgcatctta aacgtcacga tttaccgtgc actggcgtgg
     7561 ttcacggttt ttccggcagc ctgcaacagg ccgagcggtt tgtacagctg ggctacaaaa
     7621 tcggcgtagg cggtactatc acctatccac gcgccagtaa aacccgcgat gtcatcgcaa
     7681 aattaccgct ggcatcgttg ttgctggaaa ccgacgcgcc ggatatgccg ctcaacggct
     7741 ttcaggggca gcctaaccga ccggaacagg ctgtgcgggt gtttgacgtg ctttgcgagt
     7801 tgcgcccgga accagaagat gagattgccg aagtgttgct taataatacg tatgcgttgt
     7861 ttagcgttag tgggtagcct acccttcgta ggcagcatcc ggcagtcggc gcataatgcc
     7921 tgatgcgacg cttaacgcgt cttatcatgc ctacagg
//
LOCUS       DAZRUF010000188         7890 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZRUF010000188 DAZRUF010000000
VERSION     DAZRUF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.189"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            106..717
                     /gene="ruvA"
                     /locus_tag="ACHXOL_003292"
     CDS             106..717
                     /gene="ruvA"
                     /locus_tag="ACHXOL_003292"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR6810201.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            726..1736
                     /gene="ruvB"
                     /locus_tag="ACHXOL_003293"
     CDS             726..1736
                     /gene="ruvB"
                     /locus_tag="ACHXOL_003293"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR6810202.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1883..2668)
                     /gene="znuB"
                     /locus_tag="ACHXOL_003294"
     CDS             complement(1883..2668)
                     /gene="znuB"
                     /locus_tag="ACHXOL_003294"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR6810203.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2665..3420)
                     /gene="znuC"
                     /locus_tag="ACHXOL_003295"
     CDS             complement(2665..3420)
                     /gene="znuC"
                     /locus_tag="ACHXOL_003295"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR6810204.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3499..4431
                     /gene="znuA"
                     /locus_tag="ACHXOL_003296"
     CDS             3499..4431
                     /gene="znuA"
                     /locus_tag="ACHXOL_003296"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR6810205.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4447..5769
                     /gene="mepM"
                     /locus_tag="ACHXOL_003297"
     CDS             4447..5769
                     /gene="mepM"
                     /locus_tag="ACHXOL_003297"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR6810206.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5889..6860
                     /gene="lpxM"
                     /locus_tag="ACHXOL_003298"
                     /gene_synonym="msbB"
     CDS             5889..6860
                     /gene="lpxM"
                     /locus_tag="ACHXOL_003298"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR6810207.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6948..7890)
                     /locus_tag="ACHXOL_003299"
                     /pseudo
     CDS             complement(6948..7890)
                     /locus_tag="ACHXOL_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
BASE COUNT         2099 a         1898 c         2001 g         1892 t
ORIGIN      
        1 gttcatcgag acacctcgca agttttcttc atccttcgct ggatatctat ccagcatttt
       61 tttatcatac agcattatct ttgattcatt acgcaggagc gtcatgtgat aggcagactc
      121 agaggcatca tcattgaaaa acaacccccg ctggtgttaa ttgaagtggg cggcgtaggc
      181 tatgaagtgc atatgccgat gacctgtttt tatgaactcc ctgaagcggg tcaggaagcg
      241 atcgttttca cccactttgt ggtgcgtgaa gacgcgcaac tgctgtacgg ttttaacaat
      301 aaacaagagc gcacgttgtt taaagagtta atcaaaacca acggcgtcgg cccgaagttg
      361 gcgctggcga tcctctccgg aatgtcagcg cagcagttcg ttaatgccgt tgaacgtgaa
      421 gaagtgggag cactggtgaa actgccgggc attggtaaaa aaaccgccga gcgtttaatt
      481 gtcgaaatga aagaccgatt taaaggtttg cacggcgatc tctttacgcc agccgccgat
      541 ctggtgctca cgtcgcctgc cagcccggca accgacgatg ctgaacagga agcggttgcc
      601 gcgctggtgg cgctgggcta taaaccacaa gaagcaagcc gcatggtgag caaaatcgct
      661 cgcactgacg ccagcagtga aacattaatt cgcgaagccc tacgcgccgc gttatgaggt
      721 aaaggatgat tgaagcagac cgtctgattt ctgccggtac cactttgccg gaagatgggg
      781 cagatcgcgc cattcgccct aaattactgg aagagtatgt tggtcagccg caggttcgtt
      841 cacagatgga gattttcatc aaagcagcga aactgcgcgg cgatgccctc gatcatctgt
      901 tgatttttgg tcctccgggg ttgggtaaaa ctacgcttgc caacattgtc gccaatgaaa
      961 tgggcgttaa tttacgcacg acttctggtc cggtgctgga aaaggcgggc gatctggcag
     1021 cgatgctcac taaccttgaa ccgcatgacg tgctgtttat tgatgagatc caccgtctat
     1081 cgccagttgt tgaagaagtg ctgtacccgg caatggaaga ctaccaactg gatatcatga
     1141 ttggtgaagg tccggcggca cgctccatta aaattgattt gccgccgttt accctgattg
     1201 gtgcaaccac gcgcgcaggt tcgctgacat caccgttgcg cgaccgtttt ggtattgtgc
     1261 aacgtctgga gttttatcag gtgccggatc tgcaatatat cgtcagtcgc agcgcacgct
     1321 ttatggggct tgagatgagt gatgacggcg cgctggaagt tgctcgtcgc gctcgcggta
     1381 cgccacgtat tgccaaccgt ctgctgcgtc gagtgcgtga tttcgccgaa gtgaagcacg
     1441 atggcaccat ctcggcagat atcgctgctc aggcgctgga tatgttgaat gtcgatgctg
     1501 aaggtttcga ttatatggac cgcaaattgt tgctggcggt aatcgataag ttctttggtg
     1561 ggccggtagg tctggataac ctggcggcag ccattggcga agaacgtgaa accattgagg
     1621 atgtgctgga gccttatctg attcagcaag gctttttgca gcgtacaccg cgtgggcgta
     1681 tggcgacgac gcgggcgtgg aatcactttg gcataacgcc gccagaaatg ccgtaagtcg
     1741 gattggtcgc tcgcaccgca tccgacattg gcagtgtgcc tgatgcgacg ctattcgcgt
     1801 cttatccggc ctaccagaga tagcgaaccg taggtcggat aaggcgctcg cgccgcatcc
     1861 gacaaatgtg ttcagcgaca gattagctgg cctgcttttt catcatactg ataataaata
     1921 acagtgcagc acatagcact accgacggac ccgccggcgt atcgtaaaac gcggaaaagg
     1981 ttaaaccgcc agttactgcc accatcccca ccaaaacagc gacaccagcc atctgttccg
     2041 gcgtacgggc aaagcgacgc gcagtagctg caggaataat cagcaacgaa gtaataatca
     2101 acgcaccgac aaatttcatc gctacaccaa tcgtcaatgc cgtcaccagc atcaacaaca
     2161 atttcacgcg ctgtaatttc acaccatcaa caaacgccag atccgggcta atcgtcatcg
     2221 acagcaaatt gcgccattgc cagaacaaaa tagccaccac gatgaccacg ccaatcgcaa
     2281 tagagatgag atcttctggc gtcactgcca gcaaatcacc gaacaggtaa gccatcaaat
     2341 caacacgaat attagacatc agactaacga ccaccaggcc cagcgacagg gcactgtgcg
     2401 ccataatccc taataacgtg tcgatcgcca gctgtggacg cttctccagc cataccagac
     2461 cgcccgccag cagcagcgta acggcaatca ccgcatagaa tggattcacg tccagcaaca
     2521 aaccaaacgc gacaccaagt aatgaggcat gagccagcgt atcaccgaaa taagacatac
     2581 gacgccagac tacaaacgaa cccagcggac ccgcggcaca ggcgagcatg atcccggcta
     2641 accaaccggg aaataataat tcaatcatga gcgatcattt ccccgacgca aaacaattcg
     2701 tccctgtaaa tcgtgacgat ggttatgatg atggcgatag atacccagtt gttcagcacc
     2761 acgaggacca aacattgaaa taaactccgg atgcagggaa acaacttccg gtgtgccgga
     2821 acaacaaatg tggtgattca ggcacagcac ttcatcggtt tttgccatca ccagatgcag
     2881 atcgtgagaa accattaaaa cgccacaatc cagttcacga cgcagttggt caataaggtc
     2941 atataacgcc acctggccat ttacatccac gccttgagtg ggttcatcca gcactaataa
     3001 ttgcgggcga tttaacaatg ctcgcgctaa cagtacacgc tgcgtttcgc caccagagag
     3061 cttttgcatc ggtgcgttaa tcaggtgccc ggcctggaca cgtttcagtg caggcaaaat
     3121 atcttcttta tgtgtaccag ggcgtaagcg taaaaaacgg tttacggtca gtggcaacgt
     3181 ggtgtcgaga tacagcttct gcggtacata gccgatgcgc agttttccgt tgcgcttgat
     3241 aaccccttca tcgggtgtta ccagcccgag cactacccgt accagtgtcg acttacctgc
     3301 gccatttggc ccaagtaaag tcaaaatttt tccaggttta agttccagcg acacatcaga
     3361 gaggacgcgg cgttggccaa aagaaaccga gacattttcc aaggaaacca gacttgtcat
     3421 gttaatttta gtcttgcagt agtcatgaaa tgttataata tcacacttct catattcatt
     3481 acgatgattg gtcgcattat gttacataaa aaaacgcttc ttttcgcagc attatccgcc
     3541 gctctctggg gaggtgcaac acaggccgca gatgccgccg ttgtcgcttc gcttaaaccc
     3601 gttgggttca tcgcttctgc cattgctgat ggggtaacag aaacagaggt tttacttcct
     3661 gacggcgctt cagaacatga ttattcactg cgcccatcgg atgtaaaacg cttacagaac
     3721 gcggacttag tcgtttgggt tggcccggag atggaagcgt ttatgcaaaa accggtaagc
     3781 aaattaccag gagcgaagca ggtaacgatt gcgcagcttg aagatgtgaa accgctactg
     3841 atgaaaagta ttcacggcga tgatgatgat cacgaccacg cggaaaaaag tgacgaagat
     3901 caccatcacg gcgatttcaa catgcatctt tggctttccc cagagatagc gcgggctaca
     3961 gcggttgcaa tccatggaaa attagtggaa cttatgccgc aaagtcgagc caaacttgac
     4021 gccaacctga aggattttga ggcacaatta gcctcaaccg aaacgcaggt tggtaacgag
     4081 ctcgcgccgc tcaaggggaa aggttatttc gtttttcacg atgcttacgg ctatttcgaa
     4141 aaacagttcg gactgacacc gcttggtcat tttaccgtta accctgagat tcaacctggc
     4201 gcgcagcgtt tacatgaaat aagaacacag ttggttgagc aaaaagcaac ctgcgttttt
     4261 gctgagccac agttcaggcc agcggtcgtt gagagcgtcg cacgagggac atccgttcgt
     4321 atgggaacgt tggatcccct ggggacgaat atcaaactgg gtaaaacaag ctattcagaa
     4381 ttcctgagtc aattagccaa ccagtatgcg agctgcctga aaggagatta atgaggaagt
     4441 gattacgtgc aacagatagc ccgctctgtc gccctggcgt ttaataattt accgcgacca
     4501 caccgcgtta tgttggggtc gctcaccgtt cttactctgg ccgtcgctgt ctggcggccc
     4561 tatgtttatc accgcgatgc cacgccaatt gtcaaaacca ttgagctgga acagaacgaa
     4621 attcgttcgc tcttacctga agccagtgag ccgattgatc aagctgcaca agaagatgaa
     4681 gccattcccc aggacgaact ggatgacaaa atcgccggtg aagcgggcgt gcatgaatat
     4741 gttgtttcca ctggcgatac gctaagcagc attctcaatc agtatggtat tgatatgggt
     4801 gatatcaccc aactggctgc ggccgacaaa gaattgcgta acctgaaaat cggtcaacaa
     4861 ctctcctgga cattaaccgc ggacggcgaa ctgcagcgcc tcacctggga agtgtctcgt
     4921 cgtgaaaccc gaacctatga ccgtactgcc gctaacggtt ttaaaatgac cagcgaaatg
     4981 cagcaaggag agtgggttaa caatctgctg aaaggtaccg tcggggggag ctttgttgcc
     5041 agcgccagaa acgccggttt aaccagcgcc gaagtgagcg cagtgattaa agccatgcag
     5101 tggcaaatgg atttccgcaa actgaaaaaa ggcgatgaat ttgcggtgtt aatgtctcga
     5161 gaaatgcttg atggtaaacg tgagcaaagc cagctgctgg gcgtacgttt gcgttcagaa
     5221 ggtaaagatt attacgctat ccgcgctgaa gatggcaaat tctacgatcg taacggtact
     5281 ggtctggcga aaggattctt gcgattcccg acggcgaaac agttccgtat ctcgtctaac
     5341 tttaacccgc gtcgtactaa tccggtgacc ggtcgcgttg cagcacacag aggtgttgat
     5401 ttcgccatgc cgcagggtac gccagtgctt tcagtgggtg acggtgaagt ggtggttgcc
     5461 aaacgcagtg gcgcagcagg ttattatgtg gctattcgtc atggtcgcag ctacaccacg
     5521 cgttatatgc acttgcgcaa gattctggtg aaaccgggac agaaggtgaa acgtggcgac
     5581 cgtatcgcgc tttccggtaa taccggacgt tcaaccgggc cgcatctgca ctatgaagta
     5641 tggataaacc agcaggccgt aaacccgctg acggcaaaac tgccgcgtac cgaagggctg
     5701 accggctccg atcgtcgcga attcctggcg caggccaaag agattgtgcc gcagctacgg
     5761 tttgattaat taacatccat tcgcagccgg tacgcagtca gtatcggctt tttttatttg
     5821 gtgcggggca agttgcgccg ctacactatc accagattga tttttgcctt atccgaaact
     5881 ggaaaagcat ggaaacgaaa aaaaataata gcgaatacat tcctgagttt gataaatcct
     5941 ttcgccaccc gcgctactgg ggagcatggc tgggtgtagc agcgatggcg ggtatcgctt
     6001 taacgccgcc aaagttccgt gatcccattc tggcacggct gggacgtttt gccggacgac
     6061 tgggaaaaag ctcacgccgt cgtgcgttaa tcaatctgtc gctctgcttt ccagaacgta
     6121 gtgaagctga acgcgaagcg attgttgatg agatgtttgc caccgcgccg caagcgatgg
     6181 caatgatggc tgagttggca atacgcggac cggagaaaat tcagccgcgc gttgactggc
     6241 aagggctgga gatcatcgaa gagatgaggc gtaataacga gaaagttatc tttctggtgc
     6301 cgcacggttg ggccgtcgat attcctgcca tgctgatggc ctcgcaaggg cagaaaatgg
     6361 cagcgatgtt ccataatcag ggcaacccgg tttttgatta tgtctggaac acggtgcgtc
     6421 gtcgctttgg cggtcgtctg catgcgagaa atgacggtat taaaccattc atccagtcgg
     6481 tacgtcaggg gtactgggga tattatttac ccgatcagga tcatggccca gagcacagcg
     6541 aatttgtgga tttctttgcc acctataaag cgacgttgcc cgcgattggt cgtttgatga
     6601 aagtgtgccg tgcgcgcgtt gttccgctgt ttccgattta tgatggcaag acgcaccgtc
     6661 tgacgattca ggtgcgccca ccgatggatg atctgttaga ggcggatgat catacgattg
     6721 cgcggcggat gaatgaagaa gtcgagattt ttgttggtcc gcgaccagaa caatacacct
     6781 ggatattaaa attgctgaaa actcgcaaac cgggcgaaat ccaaccgtat aagcgtaaag
     6841 atctttatcc tatcaaataa aaaaagcctc tcaaattatt gagaggctgt actcgataaa
     6901 aaatctcaat gagtgttccc actattttga gtttttaggt tagtacgcta ttgagttaat
     6961 cgtagcgtga taatttcaaa aggtttgaac tcaagattgc aatattgatt gcaataaaaa
     7021 ctgtcatcta acttattttc ttgaagatcg acatattgaa cgtagtgata attacatact
     7081 cacgaaagca tggctgaaat ttctaatcca tgactctcat ataatcttac gattataccg
     7141 tcgccgtctt cagctaattt tattgtttcg atgataacgc tgttagtatc gatagcaaaa
     7201 aatgatagtg aagggggtaa ttcacccggg tgcggtttga cagggacgac ctgtaaggga
     7261 tgattcatta tataggcttg attaataaca ccacccacac ggtaatctcc atgatgagga
     7321 aataaggaga atatgaattt ttgtttacca atatctgcaa aattattaat caggaaatta
     7381 tcataattgt ctgtcttaac aacttcccca ggtttatgct gcgaacgcag caaagttaat
     7441 tcaagcgtct ggtctttgac acgatagcca tatttgtttt ttgctattaa agctatgcca
     7501 gtttctcgat cagaaaggtc gacccagtga tgtgctggta tttcatcttt ggcaaaagcg
     7561 aacgaatcat cgctatgcgt tggtctgtca attacaccga actgaatttg acattgtgca
     7621 agagatgttt gtactgacac cgggaatttt actttgagag aaacattagg ttcgtgccaa
     7681 tcaataaacg tttggacatc aacgcgtgga ctatcctgtg tcagggtaaa ggctaaacga
     7741 agataagagg aattgtagtg ataaatcagc gtcatcgtag ttttaggccc gctgatcaca
     7801 gttgtcattt tttcagcgag taaaacatat gattccccat cacgataatt tgaagcaaaa
     7861 tcccaggccg cgaaaaaacc tgcatcttcg
//
LOCUS       DAZRUF010000189         7874 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZRUF010000189 DAZRUF010000000
VERSION     DAZRUF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.190"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(100..363)
                     /gene="cedA"
                     /locus_tag="ACHXOL_003300"
     CDS             complement(100..363)
                     /gene="cedA"
                     /locus_tag="ACHXOL_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR6810208.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            546..2807
                     /gene="katE"
                     /locus_tag="ACHXOL_003301"
     CDS             546..2807
                     /gene="katE"
                     /locus_tag="ACHXOL_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR6810209.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3065..3823)
                     /gene="chbG"
                     /locus_tag="ACHXOL_003302"
     CDS             complement(3065..3823)
                     /gene="chbG"
                     /locus_tag="ACHXOL_003302"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR6810210.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3836..5188)
                     /gene="celF"
                     /locus_tag="ACHXOL_003303"
                     /gene_synonym="chbF"
     CDS             complement(3836..5188)
                     /gene="celF"
                     /locus_tag="ACHXOL_003303"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR6810211.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5293..6135)
                     /gene="chbR"
                     /locus_tag="ACHXOL_003304"
     CDS             complement(5293..6135)
                     /gene="chbR"
                     /locus_tag="ACHXOL_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR6810212.1"
                     /translation="MMQPVINAPEIATAREQQLFNDKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6143..6493)
                     /gene="chbA"
                     /locus_tag="ACHXOL_003305"
     CDS             complement(6143..6493)
                     /gene="chbA"
                     /locus_tag="ACHXOL_003305"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR6810213.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6544..>7874)
                     /gene="chbC"
                     /locus_tag="ACHXOL_003306"
     CDS             complement(6544..>7874)
                     /gene="chbC"
                     /locus_tag="ACHXOL_003306"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR6810214.1"
                     /translation="VLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLINNVFLS
                     FGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEERKV
                     DALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVAEMFTFIVRRN
                     WVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPLAS
                     LGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAALAA
                     GKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINEP
                     ILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNT
                     NGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANALKF"
BASE COUNT         2102 a         2065 c         1772 g         1935 t
ORIGIN      
        1 aaggcagctt tttgttacag gtggtttgaa tgaatgtagc aacgaaatgc agaatttcag
       61 gtcatgtaac tcccggcaaa accgggaagt atgtaatcct tactcagtca cttccccttc
      121 ctggcggatc tgatttgccc aacgttgggc agattcaggc acagtaaacg ccggtgagcg
      181 cagaaatgac tctcccatca gtacaaacgc aacatatttg ccacgcagca tccagacatc
      241 acgaaacgaa tccatcttta tcgcatgttc tggcggcgcg ggttccgtgc gtgggacata
      301 gctaataatc tggcggtttt gctggcggag cggtttcttc attactggct tcactaaacg
      361 catattaaaa attagaaaaa ctgtagttta gccgatttag cccctgtacg tcccgctttg
      421 cgtgtatttc ataacaccgt ttccagaata gtctccgaag cgggatctgg ctggtggtct
      481 atagttagag agttttttga ccaaaacagc ggccctttca gtaataaatt aaggagacga
      541 gttcaatgtc gcaacataac gaaaagaacc tacatcagca ccagtcacca ctacacgatt
      601 ccagcgaagc gaaaccgggg atggactcac tggcacctga ggacggatct catcgtccag
      661 cggctgaacc aacaccgcca ggtgcacaac ctaccgcccc tgggagcctg aaagcccctg
      721 atacacgtaa cgaaaaactt aattctctgg aagacgtacg caaaggcagt gaaaattatg
      781 cgctgaccac taatcagggc gtgcgcatcg ccgatgatca aaactcactg cgtgccggta
      841 accgtggtcc aacgctgctg gaagatttta ttctgcgcga gaaaatcacc cactttgacc
      901 atgagcgcat cccggaacgt attgttcatg cacgcggatc agcagctcac ggttatttcc
      961 agccatataa aagcttaagc gatatcacca aagcggattt cctctcagat ccgaacaaaa
     1021 tcaccccagt atttgttagg ttctctaccg ttcagggtgg cgctggctct gcagataccg
     1081 tgcgtgatat ccgtggtttt gccaccaagt tctatactga agagggtatt tttgacctcg
     1141 ttggcaataa cacgccaatc ttctttatcc aggatgcgca taaattcccc gattttgttc
     1201 atgcggtaaa accagaaccg cactgggcaa ttccacaagg gcaaagtgcc cacgatactt
     1261 tctgggatta tgtttctctg caacctgaaa ctctgcacaa cgtgatgtgg gcgatgtcgg
     1321 atcgcggcat cccccgcagt taccgcacca tggaaggctt cggtattcac accttccgcc
     1381 tgattaatgc cgaagggaag gcaacgtttg tacgtttcca ctggaaacca ctggcaggta
     1441 aagcctcact cgtttgggat gaagcacaaa aactcaccgg acgtgacccg gacttccacc
     1501 gccgcgagtt gtgggaagcc attgaagcag gcgattttcc ggaatacgaa ctgggcttcc
     1561 agttgattcc tgaagaagat gaattcaagt tcgacttcga tcttctcgat ccaaccaaac
     1621 tgatcccgga agaactggtg cccgttcagc gtgtcggcaa aatggtgctc aatcgcaatc
     1681 cggataactt ctttgccgaa aacgaacaag cagcattcca tccaggtcat attgttcctg
     1741 gtctggactt caccaacgat ccgctgttgc agggacgttt gttctcctat accgatacac
     1801 aaatcagtcg tcttggtggg ccgaatttcc atgagattcc gattaaccgt ccgacctgcc
     1861 cttaccacaa cttccagcgt gatggcatgc atcgtatggg gatcgacact aacccggcga
     1921 attacgaacc gaactcgatc aacgataact ggccgcgcga aacaccgccg ggaccgaaac
     1981 gcggcggttt tgaatcatat caggaacgcg tggaaggaaa taaagttcgt gagcgcagtc
     2041 catcgtttgg cgaatattat tcccatccgc gtctgttctg gctaagtcag acgccattcg
     2101 agcagcgcca tattgtcgat ggtttcagtt ttgagttaag caaagtcgtt cgtccgtata
     2161 ttcgtgagcg cgttgttgac cagctggcgc atattgatct cactctggcc caggcggtgg
     2221 cgaaaaatct cggtatcgaa ctgactgacg accagctgaa tatcacccca cctccggacg
     2281 tcaacggtct gaaaaaggat ccatccttaa gtttgtacgc cattcctgac ggtgatgtga
     2341 aaggtcgcgt ggtagcgatt ttacttaatg atgaagtgag atcggcagac cttctggcca
     2401 ttctcaaggc cctgaaggcc aaaggcgttc atgccaaact gctctactcc cgaatgggtg
     2461 aagtgactgc ggatgacggt acggtgttgc ctatagccgc tacctttgcc ggtgcacctt
     2521 cgctgacggt cgatgcggtc attgtccctt gcggcaatat cgcggatatc gctgacaacg
     2581 gcgatgccaa ctactacctg atggaagcct acaaacacct taaaccgatt gcgctggcgg
     2641 gagacgcgcg caagtttaaa gcaacaatca aggtcgctga ccagggtgaa gaagggattg
     2701 tggaagctga cagcgctgac ggtagtttta tggatgaact gctaacgctg atggcagcac
     2761 accgcgtgtg gtcacgcatt cctaagattg acaaaattcc tgcctgatgg gaggcggcgc
     2821 aattgcgccg cctcaaatga tttacatagt gcgctttgtt tatgccggat gcggcgtgaa
     2881 cgccttatcc ggcctacaaa actgtgcaaa ttcaatatat tgcaggaaac acgtaggcct
     2941 gataagcgaa gcgcatcagg cagttttgcg tttgtcagca gtctcaagcg gcgcagttac
     3001 gccgcctttg taggaattaa tcgccggatg caaggttcac gccgcatctg gcaaacatcc
     3061 tcacttacac atcccgataa ctccccaacc gataaccacg ctgagcgata gcacctttca
     3121 acgacgctga tgtcaacaca tccagctccg ttaagcgtgg gaaacagtaa gcactctgac
     3181 ggatagtatt atcgataaac gccggatgac acatcacctc cagcgaacga tcaccccgat
     3241 ggcttgcgtc atccagcact tgcaggaaca gtgcctcgct aatctcttca ccataaaacg
     3301 cgctactgaa tccctgagaa ctgcgcagat tagccggtaa atcaccagag ttagatagcg
     3361 gctgacggtc gatgcgcaac gcaatcccct cttcagccgc aaacctggca acaatcggga
     3421 aaatctgcgg gaacatatgc acatgatgat ggctatcaag atgcgtaggt ttgcgtccaa
     3481 atagctcaat gaaacgcaaa tactgactgg caagctcctg agtaatttct tccagcggta
     3541 aagcatcttc ttctgccaac tgccagatcc attttcccag cacaccatcg cgggttaacc
     3601 ccggcatagc tgtcagtggc ttgcccatag taaggacaaa gtacatccct atggccagac
     3661 tcggttcatc acgactcaac tgcaccgcat ggtcaatagc ctgcccattc accagcgccg
     3721 tcgtcgatgt gacaatccca ttgcgacagg cctcgataat gccgtagttc tgacctttgc
     3781 ttaagccaaa atcatcggca ttaacaatca gtaagcgttc cataatcagc ctcggttaat
     3841 gtgctttttt aagctctgcg atgcagtcgg caaaatttgg cagccatttc tcgtgcgcca
     3901 gaatcatctc gcgtgccagc agctcagcat cgcgatcgga atgcaccaac ggactaaggt
     3961 ttagcgccag taacacatcg ttaaattctc cgctaagtgc ggcgttgctg gcagcaatct
     4021 cgaagccttt aatggtgtga atcagcccca tcactttatc atcgaaatgc gtaatgcgcg
     4081 gatgtggcgt cgcgccatcg cgccccagct tacaggtcat ttctaccgcc cagtctgccg
     4141 gaatattatc aatatgccca tgatgcggga tattaacgta atgttcagct tgcttgtcgt
     4201 tgtagatagc gttgatcact tcgcacgctg catcagagta ataagcccca ccgcgctgtt
     4261 ccagttcttt cggcttaact ttcaactccg ggtttttata cagctcaaaa agttgtttct
     4321 cgactttctg tactacctgc gctcgtgcgc cgcctttgta gtattcgccc atttcaatag
     4381 ccagcatctc tttttgcttg aagtaataaa gcaaataaga acacggcaac agattcagag
     4441 aacgaattaa gccttcgcta aatggcagat cgaaaatatt tttaacgcca gatgctttta
     4501 actgcccgga tgccacacca tcaagcaatt cggcaaagcg cgacttgcca tttaccagca
     4561 catccttaat gaacaccata tggttgaggc cgaacaaatc gatagataaa tcatcgctgt
     4621 ctttcagcat cagaacatcg tgaataaaca tcttcatgcc gatcggaata ttacacacgc
     4681 cgataaagcg tttaaatccg gtatgacgat aaacggcttc agtaaccatt cccgccgggt
     4741 tagtgaagtt aatcacccat gcattcggac aaagttcttc gacatctttt acgatgtcaa
     4801 aaatcaccgg aatggtacgc agacctttaa acagaccgcc cgcgccgttg gtttcctggc
     4861 caagataacc atgacttaat ggaatacgtt catccagttc gcgcgccggt aattggccta
     4921 cgcgcagttg ggtagtaacg aaatcagcat ctttcaatgc ttcgcggcga tccagcgttt
     4981 tataaagctt catcgggacg ccagcgttat caatcatccg ttggcagagt tcaaaaatga
     5041 tatccagttt ctctttacca tcttcgacat ccaccagcca taattcgctg accggcaatt
     5101 cgtgataacg cttaataaat ccttccagta actccggggt atagctgctc ccgccaccaa
     5161 tagtgacgac ttttaatttc tggctcataa tttctccctt cagtacagaa tactgatatc
     5221 tggcatatct gcccccccgg acataaataa tccagcaaca ggacagatat gtgaattgtc
     5281 aggtataacg acttactgat taaattcagt caatttctta cgatagctct taggcgtaaa
     5341 ggacgttaat ttcttaaacg ttttaataaa caaactcggg ctactataac cggcctcaaa
     5401 cgcaatatcc gtcacggaat agttggtcat ttccagttgt tttttggcaa aattaatacg
     5461 gatctcatta ataatctgca ttggtgtttt gccataatat cgttgagtcg ctcgcgtcaa
     5521 atattcctgt gatttggctg acaacgtcac catattctcc agcgccgatt cactaaactg
     5581 ctctttatca tgcatctttt ctaccgtact tttcagccac tgcggtacat catcaatcac
     5641 ctgttcttcg cggtaatgac gtaaacggtt aatgacataa aaagtaacca tctcaacaaa
     5701 ctcttccagc cctgtttcgc ggaaattcaa tgaagaaatc actgtttcca cataggtgag
     5761 aaaagcgtta ttggtccggt atacctgcga agcgacaaag caataaggca acaacggcag
     5821 gtaatgctgc tcaaaaaagc gtttactgat cccaacgttc aatatacgcg tggcaccaaa
     5881 ctcataaaaa ctttgatggt gcgaacctaa cggaataaaa acaaaatcgc cccgttccag
     5941 taacacgcgc ttaccgttaa tctcctggaa ataacgcccg gttaatacca gagtaaattc
     6001 ataatagtcg tgctgatgca gcccgctgat actctcagtt ttgttataga taaacacatg
     6061 gaagtttttg tcattaaaca actgctgttc tcgggcagtg gcaatttccg gcgcgttaat
     6121 cactggctgc atcatcgact ccttatgcct tcagtttttc atgaagctca attaattcag
     6181 taatcagttc acgcgcaagc atggacgtca ttaaatgatc ctgagcgtgg accagcacca
     6241 gactcacttt catcttacct tcgcccgcat cgccttcaat cagtttcgtc tgtaccagat
     6301 gcgcttcatt caatgccatc cgtgactgat ccatcatggc ttttgctgca gcaaaatcgc
     6361 cctgcttcgc ctgtttcagt gccgcatacg ccaggctgcg cgcttgtccg gagttgatga
     6421 taagccccat caccacttct tccagctctt cagcttccgt ttgcgtatcg ggaatgttat
     6481 cgagatccat catacatcgt tcctctctat tcttaccggc acgattaccc gtaccggcat
     6541 cgattaaaat ttcagggcgt tagcgatatc ttcttcgcgc tcttctttat caatcgcgtt
     6601 ttgtgctttg ttagccacca caacaaaggg cagataaatt aacgttgcga taccaaggtt
     6661 gaagagtgca accagcaatg cggcgacgct accgttggtg ttaaagaagg ctcccagacc
     6721 ggttggcatg gtccacggtg caatattggt caccggagga atgatgccca tgtagtacgc
     6781 tgcgagggtg attgccgcca gaatcggttg taccagtaca aacgggataa acatcaccgg
     6841 gttcatgata attggcagac caaacagaat cggttcgtta atctggaaga tgccggacgg
     6901 cagcgccagt tttgccacct gacgataatc agcacggcga gaggcgataa agatagccag
     6961 gatcaggcct aaagtcgcac cactgccccc aaggaaaata aaggagtcca gcatcggctt
     7021 ggcccagata tggaaggtct taccggctgc cagcgccgct tcgacggaac catattgctg
     7081 ataggtcgcg atattttcca gcgcccacgg cgtcataatg ccgttgtcca gtgcggtcag
     7141 cgccagcgcg ccatgaatac cgaagaacca gagcagtgga acaaagatca cataggccca
     7201 gcccaccacg ctacccaacg atgccagtgg ggttgagatg gtatccataa tgatctgatg
     7261 gaagttggtg ccccaggtat tcaacgccca ggcaataatc cccatcacgg aaagaataat
     7321 aaagccggga attaatgccg agaaggaacg cgatactgaa gcaggtacgc tatcgggcaa
     7381 tttaatgacc caattgcggc ggacaataaa ggtaaacatt tctgccacca ccaggccaat
     7441 aataatcccg gagatgatat tcgctccacc taaccagttt gcgccaacca cataggcctc
     7501 accgacacta tatggggtga cggtcataaa tgctgcaacg gataacaacc cagccgccag
     7561 cgcatcgact ttacgctctt ctgccagcgc catgccaata aagaacggtg ccattaaaga
     7621 cattatccct aatgttccgt tatatacgtt gccgccaata cctttcaggc cattaagtgt
     7681 ttcaatggtt gaggcgtcga ggcgaatacc taaggaataa aaaaacgccc cctccccaaa
     7741 gcttagaaaa acgttgttaa ttaatacaaa catggccccc gcaagggtta acggcattaa
     7801 gcgaataaag ccatttttga ttgcattaac gtgtggctgc tttcctattt taactgcaaa
     7861 agggaggagt acct
//
LOCUS       DAZRUF010000190         7786 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZRUF010000190 DAZRUF010000000
VERSION     DAZRUF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7786
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.191"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            124..852
                     /gene="gadW"
                     /locus_tag="ACHXOL_003307"
     CDS             124..852
                     /gene="gadW"
                     /locus_tag="ACHXOL_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR6810215.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1215..4328)
                     /gene="mdtF"
                     /locus_tag="ACHXOL_003308"
     CDS             complement(1215..4328)
                     /gene="mdtF"
                     /locus_tag="ACHXOL_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR6810216.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4353..5462)
                     /gene="mdtE"
                     /locus_tag="ACHXOL_003309"
     CDS             complement(4353..5462)
                     /gene="mdtE"
                     /locus_tag="ACHXOL_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR6810217.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5570..5848
                     /locus_tag="ACHXOL_003310"
     CDS             5570..5848
                     /locus_tag="ACHXOL_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810218.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5849..6376)
                     /gene="gadE"
                     /locus_tag="ACHXOL_003311"
     CDS             complement(5849..6376)
                     /gene="gadE"
                     /locus_tag="ACHXOL_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR6810219.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7175..7747)
                     /gene="hdeD"
                     /locus_tag="ACHXOL_003312"
     CDS             complement(7175..7747)
                     /gene="hdeD"
                     /locus_tag="ACHXOL_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR6810220.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2060 a         1864 c         1836 g         2026 t
ORIGIN      
        1 tactttgtgg ataaattttg gtcctaccaa atctggcagt tttttcgcta agaaacagtc
       61 tggcaacatt tcattagtat actgaaattg aaataatcgc agtatgaaat ataagggata
      121 atcatgactc atgtctgctc ggtgatcctc attcgtcgtt cattcgatat ttatcatgaa
      181 cagcataaaa tatcgctgca taacgagagt atcgtgctgc tggagaaaaa tttggcagac
      241 gattttgcgt tttgttcacc ggatacgcga cgactggata tcgatgagct gacagtttgc
      301 cattacttac aaaatattcg tcagctacca cgcaatttag ggttacatag caaagaccgt
      361 ctattaatta accagtcacc ccccatgccg ctggtgacgg cgatttttga tagcttcaat
      421 gaatccgggg taaattcacc gatactgagc aatatgctct acctttcctg tttatcgatg
      481 ttttctcata agaaagaact gatcccctta cttttcaata gcatcagcac tgtttcagga
      541 aaagttgaac gccttattag ctttgatatc gccaaacgtt ggtatctgcg cgatatcgcg
      601 gaaagaatgt ataccagcga gagtctcata aaaaaaaagt tgcaggatga aaatacctgt
      661 ttcagtaaaa tattactcgc ttccaggatg tcgatggcca gacgattact cgagttacgt
      721 caaattcctc tgcatactat tgcagaaaaa tgtggctata gcagtacgtc gtactttata
      781 aacacatttc gacaatatta tggtgtaacg ccacatcagt ttgcgcaaca ttcgccaggt
      841 accttttcct gacatatttt gcatttgaat attggtcagg atctcacacc tgcttcatgt
      901 gaaactcttc cctgatgatt tctgccgggc taccggctag ttctctttcg cagtttgcca
      961 ggcggtatag gtattttcag gaggaaaaga ctcggcatgt ttgggattat taagctgaca
     1021 attcatacga ttacgggaat gtgggtcagt attgtgctgt tcaaattgat gacgaatggc
     1081 tggagtggtt tctactttca gtgctgtgta ctaagcctgg tattcctgac ggttagctgg
     1141 ttgttgagtg gtgaatggtt agcaggaaag agtaaggctg aaccttcacg ttcaacctta
     1201 ctctcattta cacgttacgc ttttttaaag cgggcaaaga gatgttccac tacaacgaaa
     1261 aagaccggaa cgaagtaaat tgccagcact gttgcggcaa acatcccgcc catcacgccg
     1321 gtacctaccg cgttttgcgc gccagaaccg gcaccatgac tgataaccag cggcagcacg
     1381 ccgagaataa aggcgagaga ggtcatcagg attgggcgta aacgcatccg cgccgcttcg
     1441 atgattgcct ctatcggcgt tttcccttct ttctgcatca tctcaacggc aaattcgaca
     1501 atcaggatgg cgttcttggc ggaaagcccg atggtggtca gcaaaccaac ctggaagtag
     1561 acgtcattac ttaagccgcg cagatcggtg gccagtaatg cgccaacgac gcctaacgga
     1621 acaaccaaca tcaccgagaa cggaattgac cagctctcat agagtgcggc gagggcgagg
     1681 aacaccacga ccagtgaaat ggcatacagt gcaggagcct ggtttgagga taacgcttcc
     1741 tgatacgaca gtccggtcca tgagtagccg acgcctgccg gaagtttagc gaccaggtct
     1801 gccataaatt tcatagcgtc accggtactt ttcccggccg ccgcttcacc taaaatctcc
     1861 attgacggga tgccgttgta gcgttccaat cgcggtgaac cataggtcca ttcggtagac
     1921 gagtaggcag aaagcggtgc catcgtgcca gaggcgttgc gtacatacca ttggttgatg
     1981 ttatccggca acatacggaa cggcgtgcct gcctggacat acactttttt cacccgcccc
     2041 tggttgagga agtcgttcac gtagctgctg ccgaaggcgg tggaaattgt ctggttgata
     2101 tcagacagcg ccacacccat tgcctcagct ttcgcggcgt tgacgttcac tttgaacatc
     2161 ggcgtatctt ccaggccgtt cgggcgtacc ccggtgacct ggtcaggcga ttgcgctgcc
     2221 agtgataaca gctcgtttcg tgcctgggtt agtttttcgt gccccaggtt accgttgtcc
     2281 agcagttcca tatcaaaacc tgacgcggta cccagttcag ccaccgcggg taagttgaac
     2341 gggaagacga cggctttatt gatactgctt aacgcaatca ttgcccgctg aatgatcgcg
     2401 gtaaccgagt tttcctcacc gacacgttca gaccacggct tgagactgat aaacgccagg
     2461 ccgttgtttt gcccctggcc gctgaagcca aagccgccaa cggtaaacac cgactgaaca
     2521 ttatctttct cttttgtaag ataataatcc gtcacctgtt gcagcacttt cgtggtgtta
     2581 accatggtgg caccggaagg taactgcgcg gtggtcataa ataccccctg atcctcttct
     2641 ggtaagaaag aggtcggtgt acgcaggaac agcaccgcca tcccggcgca aatcagcagg
     2701 tagaccacca tgtagcgacc ggtacaacgc aacagcgagc gggtgctatc ggtatagtgc
     2761 tgagttgatt tttcaaacag cgtgttgaag cgtgcgaaca gggcgttagg tttgtgaccg
     2821 ccttccggcg cggctttcag aatggtggcg cacagggcag gggtcaggct cattgccaca
     2881 aatactgaaa gcagcatgga ggagatcagc gtgatggaga actggcggta gatctccccg
     2941 gttgcgccgc tcataaaggc catcggcata aacactgcgg aaagaacaac agcaataccg
     3001 accagcgcac gttggatctg ccccatcgat ttatgcgtcg cttccttcgg tggtagctta
     3061 tcttccgcaa tgacacgctc gacgttctcc accaccacga tggcgtcatc caccagtaac
     3121 cctatcgcca gcaccatccc gaacatagtt aacgtgttga tggtgaaacc gaccgccgac
     3181 aagatcgcaa acgtcccgag aataaccacc ggtacggcaa tcgtcgggat gattgtggca
     3241 cggaaattct gcaaaaacag atacatgacc aggaagacga ggatgatagc ctcaaccagt
     3301 gttttaaaaa cttcctgaat agaaatttcg ataaacggcg tggtgtcgta aggataaacc
     3361 gtcttcagac ttgcggggaa ataggctgat aagcggttca gctcctcttt gactgcccgc
     3421 gaggtatcca gggcgtttgc tccggtagcc agtttgatgg cgatcccggc agcaggtttg
     3481 ccgttatagc gcgccacggt ggaataatct tccgctccaa gttcgacgcg ggcgacatca
     3541 cgcagcagca cttgcgaacc atcttgctga actttcaaca ggattttgcc aaattcttcc
     3601 ggcgtttgca gacgcgtctg cacaatgatc gaggcgttta gttgctggtc tgccgcctgt
     3661 ggcatgccac ccagttgacc accggaaatc tggttgtttt gcaccttaat ctgggaaata
     3721 acatcggaag gtaccagatt gtatttattg agtttttgcg ggtccagcca gatacgcatg
     3781 gcgtactcgg aaccaaagag ctgtacgcta ccaacgcccg cggtacggct tagcgggtct
     3841 ttgatattag acgctacata gtccgcgata tcgtactggt tgaggctgcc gttatcagaa
     3901 ataaacgccg ctaccatcag aatattactg ctcgacttat caacgctaat cccctgctgc
     3961 tgcaccgctt caggtaatga aggcatagcg agttgcagtt tattttgcac ctgaacctgt
     4021 gcgatatcag gagatgtccc agtctcgaag gtcagagtga tagaggcatt acccgccgca
     4081 tcactggttg aagacatgta catcaggcca tcaagcccat tcatattttg ctcaatcacc
     4141 tgagtgaccg agtcttctac cgtttgcgca tcggcacctg gataggtagc gctgacggta
     4201 atggtcggtg gcgcaatctg cggatactgc gcaaccggta agttcatgat cgccagacca
     4261 cctgcaagca tcataataat ggcaagtacc caggcaaaaa ccgggcgatc aataaaatag
     4321 ttagccatga aagtcccctt aagcctgcaa cgttattgtt tcgattcggt gctggcgttt
     4381 tcctggctgg aggaaattgc tcgtgctttg atacccggac gaatgcgttg caaaccggaa
     4441 acgatcaccc gatcgccagc ctgcaagcca gaggtgacaa cccactggtc gccgatggct
     4501 ttgctggctt caatttcgcg tagctgcaca acatcgtctt tatccagaat gagcgccgtt
     4561 gctttaccct gggcgttgtg agtgacgcct tcctgcggca ccagtaatac attctggcgg
     4621 ctaccttcat ccactaatgc cgtgacgtac atgccaggca gcaagtcacc atttgggttg
     4681 gggaaaatcg cccgtaacgt cacggagccc gtggtttcat ccaccgtcgg gtcggagaat
     4741 ttcagtgtgc cggtctggct gtagcgttta ccattttcca gattgagctg tactggcgta
     4801 ctgccctgaa cctgtttgat ttgcccactg gcgacctctt ctttcatgcg caagaaatcc
     4861 tgcaccgact gcgtgagatc gacataaatc gggtccagac gttgtacggt aaccagcgaa
     4921 tctgcctgat tagcggtaac gagtgcgccg acggtcaccg acgatttccc gctgacgccc
     4981 gtaatcggcg aggtgacatt cgcgtattgc agattgatcg tcgcctgttc aacagccgct
     5041 ttggcgacgg tgacattggc ttctgcttca ttcagctggg tgcgcgcggt gtcgtaatcc
     5101 tgacggctaa cgtagttggt cttcagcaac gatgcctggc ggttaaaggt gatgcgggca
     5161 ttgctggcgg tagagagcgc tttcgccagc gagcctttgg cggagtttag ctcggcctgt
     5221 aaaggtgcag gatcaatctg atacagcgaa tcgccctggt tcactttatc gccttcgata
     5281 aagttgcgtt taatgataat accgcccacc tggggacgta tctcggcaac ttcataagga
     5341 acggttctac cgggcaattc gctcaacaca ttgaccgaac cgggggagag tgtgacgaca
     5401 ccgacctcag gcgtcatggc ggcggcgttt tccgccgatt tgtcatcgca ggcggtgagc
     5461 atcgcgccgc agaataacaa cggtattaac agctttcttc ttctgttcat tttagtccct
     5521 gaaaattctt gagaaataac ggttctttaa attgcgcgcg aggagagata tgagggattt
     5581 acagaccagc ggcatagtgg ggttgtcagc cagtaaggtg ggatacaggc acagtgatcg
     5641 acatggtgag gtcaacgaca aaaaaaatgt tgccgttctg ccaacagttc ctgccagcat
     5701 tcgggcaacg aagggttcta ctggtggata cacatacctg gggaataaag tcgttttagc
     5761 ctctccttta gtaatcaccg ggggtaatga aatcagcatc tgcatccctc gtcatgccag
     5821 ccatcaattt cagttgctta tgtcctgact aaaaataaga tgtgataccc agggtgacga
     5881 tgtcgctcat acgtcttagt ttcagcttgt acataatgtt gaatttctga atctttaatg
     5941 tttgttgcga aatattgagc gtcgacgtga tattgctttc atttttattt ttcagaatca
     6001 gttgcataat tttgaactga ttatgtgaaa aacataattg tgaattctta tggggcaagt
     6061 gtttaccata agtaatctca agaagatttt tgatactttc tttgcggcta acaaaataaa
     6121 taggcgcttt agcttttagt ttactgatgt gataaggtgc caaaacaata attctctcgg
     6181 catctaattt ctccagaaat ttaatcgctt cttcatcaag gatatgattg tgataggtat
     6241 caatgataac cttgaagggt ttattgccta cttttttgat ctctgacagg gaattgattt
     6301 ttatcatttc gtgattatct ttcaactgcc aaaagccctg taaaagaaaa gaatctttcg
     6361 tcatgagaaa aatcataact tgctccttag ccgttatcgt ttcgaacatg tcatcctttt
     6421 cattatttac atccttgtcc gaatcgttgt tcaatatagt aaacgcctat aagaacttta
     6481 cccttcgtga actaatacgt ctattaacac taatcaatac ttcagattta tccatcacgc
     6541 actccatata ttgatcctaa gtaagattcc tggttgttat cagcttgtaa atagtaaaca
     6601 aaaagttaac gtttgcggat tactgcccaa gaataagtta tcaataacca gtttgtgatc
     6661 tctgaagaat attactaaag ttaaaatgta atccgattta aatatcgagt ctccttgttt
     6721 cgacttaagc tggcaattgg attgccagct ttcttttaac gcattgaaat tacctgagta
     6781 aataacgcat tcatgtcttc aggtttttat atagcgtctg gagacacgga tatttatgca
     6841 atgtaatcaa cgccagttgc aaaaaaatga acccggatta cttatttaac cgcccatagc
     6901 atcaacgctt acggaactct ttgttcactt tcccacagca cttttaaaac attaaacctg
     6961 actacggaaa atatcagcca tgataatgtt tgaaatggct aatttgccat ggagtgaaaa
     7021 aaattagatg aaattcagta ggttgaaata atcactagca ggtaattatt tcaatgatag
     7081 tgcgcaattg atctacaaca ctccgtagcg gagagagtat taatcggatc atagtcatat
     7141 caagtgacta tgatccgggt gacaaccggg ataattattg ctgcttaacg aacaaactgg
     7201 cgaagctgaa caggctggcg gcgctaaata tcagttcaat tcccaccagt gtggaaacca
     7261 gcgttacaga caccatcggc gttgcaccaa ggaatatcca ggcaatgacg atatccagca
     7321 caccaataac gagctgtagc cagctgcctt tcattgaacg ctgacgatac caactcatca
     7381 ggcgaataac ccctgcaaca cagaacaaac cggcaataaa tgccgcaatg gcaaaaatgc
     7441 ccagctccgg tgcgcggatg aagaaatagc cgatcaataa ataggcgact gcgacgagga
     7501 aaccggataa taccggccag aaattatgac tgcggttgct gaataacccg acaataagcg
     7561 caatgcccga gcagattaat aatgcaccca ctactgtgct taaaatatcg ccagagacga
     7621 acgggaaact gatacacagc aacccgacga taaacagtag cacggcaata aactggattg
     7681 ctctgcgatg ttttttaagc atctccagat caaacttcaa aattgttgcc ttatctatat
     7741 ataacataga accaccctat aaaattaaga agaaaatccc ctgcta
//
LOCUS       DAZRUF010000191         7781 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZRUF010000191 DAZRUF010000000
VERSION     DAZRUF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.192"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..1784)
                     /locus_tag="ACHXOL_003313"
     CDS             complement(<1..1784)
                     /locus_tag="ACHXOL_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR6810221.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQNR"
     gene            complement(1925..3265)
                     /gene="gntU"
                     /locus_tag="ACHXOL_003314"
     CDS             complement(1925..3265)
                     /gene="gntU"
                     /locus_tag="ACHXOL_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR6810222.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3269..3796)
                     /gene="gntK"
                     /locus_tag="ACHXOL_003315"
     CDS             complement(3269..3796)
                     /gene="gntK"
                     /locus_tag="ACHXOL_003315"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR6810223.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3935..4930)
                     /gene="gntR"
                     /locus_tag="ACHXOL_003316"
     CDS             complement(3935..4930)
                     /gene="gntR"
                     /locus_tag="ACHXOL_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR6810224.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5154..5849)
                     /gene="yhhW"
                     /locus_tag="ACHXOL_003317"
     CDS             complement(5154..5849)
                     /gene="yhhW"
                     /locus_tag="ACHXOL_003317"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR6810225.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5972..7009)
                     /locus_tag="ACHXOL_003318"
     CDS             complement(5972..7009)
                     /locus_tag="ACHXOL_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR6810226.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7343..>7781
                     /gene="yhhY"
                     /locus_tag="ACHXOL_003319"
     CDS             7343..>7781
                     /gene="yhhY"
                     /locus_tag="ACHXOL_003319"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR6810227.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1980 a         2104 c         1872 g         1825 t
ORIGIN      
        1 aaacggtttt gctgcatttt ctgcggtgga ttgtccacga ctttttcaat ggcatcaata
       61 aaaggtaagc caagtttcgc cgccagtcgc cgggcaaaat caggaaccag gtcgaggtgc
      121 tttaatgaag gaacgcagca tacccaggtt ggttcaggat gcggattcca gcgttggcga
      181 accatttccg cacatgcctc caccagctca tcactgaatc gccccgtttt tttctcctgt
      241 gctacctgct ttccccaccc ggaatctccc caacgagaaa ggactcgccc cgtagatcct
      301 tgtaaacccg caggcaagtt ccctttaaat ccgtattgcg taaaagctcc agatgctacc
      361 tgtttattga gatttaatgg caggtcagcg tgtttaataa ataaatttgc cgcatggagt
      421 aagccgctat caatatcggg cgacaacaac ggatgctgaa gacaactgct acatttacca
      481 caacgttcgg cacttggctc atcgagtgcg cggcgcagaa attgcatttt gcactccttg
      541 gtcgtgatat agctttcaac atctgccagc tcattttttc tctgattcat caaatggtta
      601 attcgttcat ggtcaggaga gaaactgacg atagttctgc gccataattt ctcggtatac
      661 accacaggcg atggattttc agctaccaat aatttcagtg ctttttctat ttgcccataa
      721 cggagattcg tccgttgttc aatgcctcgt agggtaagac cgtcattctc gctaagtacg
      781 ttgagtattt catgaatttg cgcctccgca ggaaaggcgc tttcacggaa gaatttatga
      841 atagcgcggt cttcaccacc acaaagcaat atgccaactg cggaatctat agcacgcccg
      901 gcacgcccta cctgttggta gtaaccgaca atagaccccg gcatttgata atgaatgacg
      961 aaacctaaat caggtttatc aaaccccata cctaatgccg tcgtcgcgac cagcactttg
     1021 attttatttg ccagcagcgc ctgctccaga tattccctgg cagtattaga atcttccgcg
     1081 ccttcacaag tcacgccgct gtagtaagca aatgcactga taccatttgt ccttaaccac
     1141 tcggcgacaa gttcggcatc gcgagtagtc agggtataaa caattcccga tttggaaaac
     1201 tgagggataa ccgttgctaa ccatgccaga cgggatgact gttcacctaa gactaaggca
     1261 tcaagcgcca aactttcgcg agccagcgtt ccacgctgaa tcacaatgtc acccaattgc
     1321 tgacggatat cctcaacgac acgattattc gctgtcgcgg ttgtacccag aacaggggta
     1381 ttcgcagttt caagaaaatc ctgattcgcc aaccgttcag gtgaaatcag cagacaatct
     1441 acaccacccc gcagcaactt atcgctaatg cgctgccatt cttccctgtt tgtagaattc
     1501 aacgtttcag ccgtaatacc taaccgttca gcggcggcaa cctggttacg catcagggca
     1561 agcaaaggag agatgataat tgtcgggcca gcgccgcgat cgcggaagat tttactggcg
     1621 atgaagtaca cggcactttt cccccagcca gtgcgttgga caaccagcag ttttcgccgc
     1681 tgattaacca ccgcatctat agcttcccat tgcccttccc tgaaagtagc gctggtgtta
     1741 cttaacatcc gctgtaacag caattcagct ccgtgtttct ccatcatctt ctccctcgtc
     1801 cgttaacgca gtgattagaa cggataatgt tccggtcttt ttgcgagaga gatcaaaaag
     1861 atgaagacag gagataagag aaatgaatta ctgcgagcaa gctcgcagta atttgtcgca
     1921 gtaattaact caacagctgg aacgcaatca tcccaacaat ggcaccaaca gtgccgagga
     1981 tggtttccat catggtccag gttttcagcg tttcggcttc ggtcgcgccg gtaaatttac
     2041 cgaacaacca gaaaccagcg tcgttaacgt ggctgacaac aatcgaacca ccggcgatac
     2101 aaatcgacag cgccgccatt tgcgcaccgg aattgttcag ttgttcaatg accggcatca
     2161 ccagccccac cgccgttaaa caggcaacgg tggcagaccc ctgaatgatg cgcactgcag
     2221 ctgccagcac gaagcaggtg atagcaatcg gcaggcccat gccggttaac gcttcgccca
     2281 gtgccggacc tacgccagag tcaaccagca cctgtttgaa cacgccgcct gcaccaatca
     2341 ccagcagaat gatccccgcc ggttgcagcg cgtgaccgca aatctccatc actttgtctt
     2401 ttggcatgcc ctgacgcatt gccaggccat aaatcgctac cagacaagca accagaatcg
     2461 cggtaaacgg atgaccaata aactcgaacc attcgtaagc ggtagagcct tctggcacaa
     2521 aacgcgcggc aatggttttc agccccacca gcaccagcgg caacaggatc agcgacaggc
     2581 tgaatccgaa agatggcatt ttaccttcgc cgagatgcgg ctcgctgatg tcgtcaggaa
     2641 tatgcagctc aacgtaacgg ctgatgaagt taccccacag cggcccggca ataatcattc
     2701 ccggaattgc cgcacacagg ccaatcagga tcatccagcc aaaatcggcg ttcatctgcg
     2761 atgccagcag cattggcgct ggccctggca ccaggaacgc cgccgctgcc gccacgcctg
     2821 cgaataatgg gattatcagc ttcaccaggt tcgtatcggt gtggcgcgcc attgagaaag
     2881 caacgctaat cagcagaaca atcgccactt caaagaacag cggtagcgca cagaccagcc
     2941 ccgcaaggcc gatggcataa tgcgcgcggc tgtgaccgaa ggatttgagc attttgacgg
     3001 caatctgatc gactgcgccg gtttcatgta agatcttgcc aaacatagct cccagggcga
     3061 caaccaccgc caggaagccg agggtgcctc ccatcccttt ttccatcgtc gctgcgattt
     3121 tatcgagcgg cataccggaa aaaaggccag cccccatgga caccaccatc aaagccagga
     3181 aagcgtgcat acgcgccttc atgactaaaa acagcagcag taaaacagac cctactgctg
     3241 ttaaaacaag cgttaatgta gtcactactt atttgccttt tttaataacc tcaatggtgc
     3301 ttgccacaac accttccagc ggttgatcga tatccaccac cagtacatcg gtttcgtccg
     3361 cacccggctc ctgcagcgtt tcaaactgcg tcaccaacat ttgggtttta aagaaatggc
     3421 ctttgcgcgc tttcaggcgg ctttcaatca cgtcaaaatc gcctttcagg tagatgaaag
     3481 agagattcgg attaccttca cgcagcaagt cgcgatagtg ttttttcaat gcagaacaga
     3541 cgatcagtga cactttatta gtgcgctgca tagcaaacgc ggcgtcgttc agcgcctgca
     3601 accagggttt gcgatcgtcg tcattcagcg gttcgccaga cgccattttt tcgatattgc
     3661 gccgtggatg gaggaaatcg ccatcaagaa acgcggcatg aagttgatgc gccacttcac
     3721 tggcgaccgc agatttgccg ctgcccgata cgcccatcaa gacgtaaatg tggtgatcat
     3781 ggttagtcgt gctcaaagtg gtgcccccac aatacaagaa ttaaaaatgt tacgggtaac
     3841 tgttatcggt aacattgtcc agccggacaa tatcagaagc aatatccatc cgtgccttaa
     3901 gtgtataagt gtgagctact tcaaatttgt aggcttaaat agatccgccc ggtgacaagg
     3961 tgaaacctaa atctaacatt ttcggtgtca cagattcgcc acgaatacgc gccagcaggc
     4021 gttcagcgcc aatactgccc atccgctcac gcggcgtcag cacgctcgct aaccgcggct
     4081 ccatcacctg gccaatgtca tgaccgtgga aaccggcaat cgccatatcg tcaggaacct
     4141 tcaatcccag acgctgacat tcaaacgccg cgccgaccgc caggtcatcg ttggtacaga
     4201 acacgccatc cagctgcgga tattcccgcc gcgcctggcg aatcagttca ataccggaag
     4261 agtaagaaga agattgctca accatcacgc tatacggcac cagacctgca tccagcatcg
     4321 cctgttcgta tcccttctgt ttgatgatag tacgttcgtc gagacgcgcg ccgagatagg
     4381 caatgtggca atgcccgcga gcaataatgg cggtggtcat ctggcgtgcc gcttcaaagt
     4441 tatcaaaacc gacggcgata tcgaggcacg gcgactggct gtccatgagt tccaccaccg
     4501 ggatccccgc cacttcaatc atctttaagg tgcgcggcgt gtgggtacgt tcggtgagga
     4561 tcagtccgtc gatattccag gagagcatgg attcgagacg ttcttgctcc atttccggtt
     4621 tataaccgta gtgagccagc atggtctgat aaccgtgcgc gtcggtgacg ctttcgattc
     4681 cgcgtaatac ttccgcgaaa acctggttgg tgagagaagg taacaggacg ccaatcgccc
     4741 ggctggtggc gttagagagg atatcgggcg cacgattggg aatatagccc agttcatcaa
     4801 gagcggcggc aatcttgccg cgtagagcga cggaaacctg ctccgggttg cgtaaaaaac
     4861 ggctgaccgt cattttggtc acgcctacac ggtcagccac atcctgaagt acgggtcttt
     4921 tctttttcat cgtcctgaag gtacaaaaga gatagattct cttagtttaa cacggacgca
     4981 tcagaacctt cccggggaaa acagtgcttg tttacaatta ttagatatac actaaatcaa
     5041 ttgcatcgca ttgtgctaac gacgcggtaa aaccccggaa gcgtgcatca gcacgcgacc
     5101 ggagcgagcg aggaagcctg caaaaaggca ggcttcaaag atgccgttga gttttaaacc
     5161 ggcggcagat cgaacagtaa cacttcgctg tcgctatccg catggatgga gattgcctgc
     5221 tcatcccaga ttgccagacc atcgctggtc gaggctttca cgccgttaat ggtgacattg
     5281 cctttcacca cctggatcca gacgcggcgt tcagcggcaa tctgatgcac cgactgctca
     5341 tctttcagca acgcccagcg gtacagttcc atatcctgat gcactttcaa tgaaccatct
     5401 cgcgcatccg gcgagagcac cagctgtttg ccctgtacgg catcgaagcg acgctgttca
     5461 taacgcggcg taataccgtt ttcttcaggc atgatccaga tctgatacag atgcagacgc
     5521 tcggtgctgc ttgggttgta ctctgagtga cgaatacccg tacccgcact cataatctgg
     5581 aactcacccg ccggaacctg ctctttattg cccatgctgt cctgatgctc aacagtacct
     5641 tccagcacgt aggtcaaaat ttccatatct ttatgcgggt gagtgccgaa gccctgccct
     5701 gcttcaatca cgtcgtcgtt aatcacgcgc agcgcggaga agcccataaa gttcggatcg
     5761 taatagttgg caaaagagaa agtatgccag gagtccagcc agccatgatt tgcatgacca
     5821 cgttcatttg ctttgcgtaa gtagatcatt tgtattcgcc ccatgaatga tttcgatggg
     5881 cttagtgtgg acccgatctg cctgggatga tagagggtga aaattgaccc ctctgttcaa
     5941 aaaaattgaa ctattcgagg ggccatcaaa cttacttcgc gagagtcact gtggagggag
     6001 aggcttgctc aaatccgcgt tcaaggattt ccagattggt aagaacttca gattccttga
     6061 cgtaatttgg cgcaccgtgg gtgatggttt gatacaacgc atcataaacg cgcccgtaat
     6121 cgcccatctc cggcttcatc tcttctctga ccgtcacgcc ctcgtcattg acatactcca
     6181 gcacaccgac cgaatcatcc gctgcgaatc ccggttcgcc cggcataata ttagccttca
     6241 ggctggtttc ctgctggtcg ataccgtatt taataaacga acctttctta ccgtgaacga
     6301 taaatttcgg ataatcgatt ttcaccagat ggctggtttt gacgatggct ttcaggtcgc
     6361 cataaaacag ttgcgcttca aaggtgtcgt caggattggc tttattacgc aggctgcaga
     6421 tgtcataagc gacgtgatcc gggcgaccga acagagaaat aatctggtcc atcgtatgca
     6481 caccaaggcc atagaacgcg ccatcctgcg gcaacccagg tttggtttct gccaccgggc
     6541 ggtaatagtc aaaatggctt tccacttcaa caatctctcc cagcttaccg ctttcaatcg
     6601 cttttttcgc cgtcaggaag caggaatcaa agcgacgatt ctgatatggc gtgacggtca
     6661 gccctttgct tttcgccagt gcaaacagct ctttcgcctg cgcaagtgtc ggagtgaacg
     6721 gtttttcgac cagcacattt ttcccggctt ccagcgcgcg cttcgcgtac tcgaagtggc
     6781 tgtccgcatg ggtgcagaca acaaccaact taacatcggg atcgtttagc acttcgtcga
     6841 gatcgctggt gaaatggata tgagagtaaa tgggagcctg ttcttccggc ttcgcatggc
     6901 gacgaaaaat atgcgcgaca tgccagctat ccttgcggtt aagtacatac ggcagatggt
     6961 aacgggtggt gcttttgccg aagccaataa aggcgcagtt gatgaccatg atttcgtcct
     7021 ttttaaggtg gttatttaca ccttagcgca aagcggacgt ggttcctact ggagtgcgca
     7081 taacgaacac aagcactccc gtggataaat tgagaacgaa agatcaaaaa aaaagccagc
     7141 acccggctgg ctaagtaata ctggaagcaa tgtgagcaat gtcgtgcttt caggttctcc
     7201 gcgagggtct tcctgatcgc gagacaataa taatcattct cattcgcact tgtccaacac
     7261 tttttgcaaa aaaaatgcat ttgactcgca tttgaaagtc aatgatgttg aaagggacat
     7321 ttaccccaaa gaggacaaag gaatgagtga gatagtaata cgccacgcag aaacacggga
     7381 ttacgaggcc atcaggcaga ttcacgccca gccggaggtg tattgcaaca cactacaggt
     7441 gcctcatcct tccgatcata tgtggcagga gcgactcgcc gatcgtcccg gcatcaagca
     7501 actcgtcgcc tgtattgatg gagacgtcgt gggccatctc accattgacg tgcaacagca
     7561 cccacgccgc agtcatgttg ccgattttgg tatctgtgtc gactctcgtt ggaagaaccg
     7621 cggcgtcgcc agcgccctga tgcgagagat gattgaaatg tgcgacaact ggttgcgggt
     7681 agatcgcatt gaactaaccg tgtttgtcga taacgccccg gcaattaagg tctataaaaa
     7741 atacggcttt gaaattgaag ggactggtaa gaagtacgca t
//
LOCUS       DAZRUF010000192         7736 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZRUF010000192 DAZRUF010000000
VERSION     DAZRUF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.193"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            200..703
                     /gene="dps"
                     /locus_tag="ACHXOL_003320"
                     /gene_synonym="pexB"
     CDS             200..703
                     /gene="dps"
                     /locus_tag="ACHXOL_003320"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR6810228.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1107..1853
                     /gene="glnH"
                     /locus_tag="ACHXOL_003321"
     CDS             1107..1853
                     /gene="glnH"
                     /locus_tag="ACHXOL_003321"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR6810229.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1992..2651
                     /gene="glnP"
                     /locus_tag="ACHXOL_003322"
     CDS             1992..2651
                     /gene="glnP"
                     /locus_tag="ACHXOL_003322"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR6810230.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2648..3370
                     /gene="glnQ"
                     /locus_tag="ACHXOL_003323"
     CDS             2648..3370
                     /gene="glnQ"
                     /locus_tag="ACHXOL_003323"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR6810231.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3487..5712
                     /gene="ybiO"
                     /locus_tag="ACHXOL_003324"
     CDS             3487..5712
                     /gene="ybiO"
                     /locus_tag="ACHXOL_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR6810232.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(5709..6635)
                     /gene="rlmF"
                     /locus_tag="ACHXOL_003325"
     CDS             complement(5709..6635)
                     /gene="rlmF"
                     /locus_tag="ACHXOL_003325"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR6810233.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6911..7171
                     /gene="mcbA"
                     /locus_tag="ACHXOL_003326"
     CDS             6911..7171
                     /gene="mcbA"
                     /locus_tag="ACHXOL_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR6810234.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7436..>7736
                     /locus_tag="ACHXOL_003327"
     CDS             7436..>7736
                     /locus_tag="ACHXOL_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR6810235.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         1877 a         1975 c         1943 g         1941 t
ORIGIN      
        1 tccgaaaatt cctggcgagc agataaataa gaattgttct tatcaatata tctaactcat
       61 tgaatcttta ttagttttgt ttttcacgct tgttaccact attagtgtga taggaacagc
      121 cagaatagcg gaacacatag ccggtgctat acttagtctc gttaattact gggacataac
      181 atcaagagga tatgaaatta tgagtaccgc taaattagtt aaatcaaaag cgaccaatct
      241 gctttatacc cgcaacgatg tctccgacag cgagaaaaaa gcaacagtag agttgctgaa
      301 tcgccaggtt atccagttta ttgatctttc tttgattacc aaacaagcgc actggaacat
      361 gcgcggcgct aacttcattg ccgtacatga aatgctggat ggcttccgca ccgcactgat
      421 cgatcatctg gataccatgg cagaacgtgc agtgcagctg ggcggtgtag ctctggggac
      481 cactcaagtt atcaacagca aaaccccgct gaaaagttac ccgctggaca tccacaacgt
      541 tcaggatcac ctgaaagaac tggctgaccg ttacgcaatc gtcgctaatg acgtacgcaa
      601 agcgattggc gaagcgaaag atgacgacac cgcagatatc ctgaccgccg cgtctcgcga
      661 cctggataaa ttcctgtggt ttatcgagtc taacatcgaa taaatccatc gctgatggtg
      721 cagaacttta gtacccgata aaagcggctt cctgacagga ggccgttttg ttttgcagcc
      781 cacctcaacg cacttattta gtgcatccat ctgctatctc cagctgatta agtaaatttt
      841 ttgtatccac atcatcacac aatcgttaca taaagattgt tttttcatca ggttttacgc
      901 taaataatca ctgtgttgag tgcacaattt tagcgcacca gattggtgcc ccagaatggt
      961 gcatcttcag ggtattgccc tataaatcgt gcatcacgtt tttgccgcat ctcgaaaaat
     1021 caaggagttg caaaactggc acgatttttt catatatgtg aatgtcacgc aggggatcgt
     1081 cccgtggata gaaaaaagga aatgctatga agtctgtatt aaaagtttca ctggctgcac
     1141 tgaccctggc ttttgcggtt tcttctcatg ccgcggataa aaaattagtt gtcgcgacgg
     1201 ataccgcctt cgttccgttt gaatttaaac agggcgataa atatgtgggc tttgacgttg
     1261 atctgtgggc tgccatcgct aaagagctga agctggatta cgaactgaag ccgatggatt
     1321 tcagtgggat cattccggca ctgcaaacca aaaacgtcga tctggcgctg gcgggcatta
     1381 ccatcaccga cgagcgtaaa aaagcgatcg atttctctga cggctactac aaaagcggcc
     1441 tgttagtgat ggtgaaagct aacaataacg atgtgaaaag cgtgaaagat ctcgacggga
     1501 aagtggttgc tgttaagagc ggtactggct ccgttgatta cgcgaaagca aacatcaaaa
     1561 ctaaagatct gcgtcagttc ccgaacatcg ataacgccta tatggaactg ggcaccaacc
     1621 gcgcagacgc cgttctgcac gatacgccaa acattctgta cttcatcaaa accgccggta
     1681 acggtcagtt caaagcggta ggtgactctc tggaagcgca gcaatacggt attgcgttcc
     1741 cgaaaggtag cgacgagctg cgtgacaaag tcaacggcgc gttgaaaacc ctgcgcgaga
     1801 acggaactta caacgaaatc tacaaaaaat ggttcggtac tgaaccgaaa taataacgct
     1861 acacctgtaa agcgcactgg cagttccctc tcccctctgg ggagaggatt agggtgaggg
     1921 gcgcaaaccc gctccggggc cattaattac cctgaatttg attatttaca ccacggtaac
     1981 aggaacaaca tatgcagttt gactggagtg ccatctggcc tgccattccg cttctgattg
     2041 aaggtgccaa aatgaccctg tggatttcgg tcctcggtct ggctggcggt ctggtaatcg
     2101 gattgctggc aggttttgca cgcaccttcg gtggttggat agccaaccac gtcgcgctgg
     2161 tctttattga ggtgatccgc ggcacaccta tcgtcgtcca ggtgatgttt atttatttcg
     2221 ccctgccgat ggcgtttaac gacttacgca tcgacccatt tactgcggcg gtggtcacca
     2281 tcatgatcaa ctccggcgcg tacattgcgg aaatcacgcg tggtgcggtg ctgtctattc
     2341 acaaaggttt tcgtgaagca ggactggcgc tcggtctttc acgttgggaa accattcgct
     2401 acgtcatttt accgctggca ctgcgtcgta tgctgccgcc gctgggtaac cagtggatca
     2461 tcagcattaa agacacctcg ctgtttattg tgatcggcgt ggcggaactg acccgtcagg
     2521 ggcaagaaat tattgccggt aacttccgcg cccttgagat ctggagcgcc gtggcggtgt
     2581 tctatctgat tattaccctg gtgctaagct ttattttgcg tcgtctggaa agaaggatga
     2641 aaatcctgtg attgaattta aaaacgtctc caagcacttt ggcccaaccc aggtgcttca
     2701 caatatcgat ttgaacattg cccagggcga agtcgtggtg attatcgggc cgtccggttc
     2761 cggtaaatcg accctgctgc gctgcatcaa caaactggaa gaaatcacct ccggcgatct
     2821 gattgtcgat ggcctgaagg ttaacgatcc gaaagttgac gagcgcctga ttcgccagga
     2881 agcaggtatg gtgttccagc agttttacct cttcccgcat ctgacggcgc tggaaaacgt
     2941 catgtttggt ccgctacgcg tgcgtggcgc gaacaaagaa gaggcggaaa aactggcgcg
     3001 tgagctgctg gcgaaagtcg gtctggcaga acgtgcacat cactaccctt ccgaactttc
     3061 tggtggtcaa cagcagcgtg tggcgattgc ccgcgcgctg gcggtgaagc cgaaaatgat
     3121 gctgtttgat gaaccgactt ccgctcttga cccggaactg cgccatgaag tgctgaaggt
     3181 tatgcaggat ctggctgaag aagggatgac gatggtgatc gtgacccacg aaatcggttt
     3241 tgccgagaaa gtagcttcgc ggctgatctt tatcgacaaa ggccggattg cggaagatgg
     3301 cgatccgcag gtattgatca agaacccgcc gagccagcgc ttgcaggaat ttttgcagca
     3361 cgtctcttaa cttctctgcc tccctttcac ccgaaaggga ggcacaccag attcctctca
     3421 tttaaaatcg cccctccccc agcatctata cttatctttt tgctctgttt tctcactgga
     3481 ggagtcatgc ggtggatcct gttcatcctc ttctgcctgc tgggcgcacc tgcccacgcg
     3541 gtatccatac ccggcgttac aaccacaacg acaacggact caacgactga accggccccg
     3601 gaaccggata tcgaacaaaa aaaagcggcc tatggcgcac tggcggatgt gctggataac
     3661 gacacctcgc gtaaagaatt gatcgaccag ttgcgtactg tcgccgcgac gccccctgcg
     3721 gaaccggtcc cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag
     3781 gtcaccgaag tcagccgcca ttatggtgaa gccctttccg cccgcttcgg gcaactttat
     3841 cgcaatatca ccggctcccc gcataagcca tttaatccac aaaccttcag caatgcgctg
     3901 agccattttt caatgttagc ggtattagtg tttggttttt actggctgat tcgcctgtgc
     3961 gcactgccgc tgtatcgcaa aatgggtcag tgggcgaggc aaaaaaatcg tgagcgcagc
     4021 aactggttgc agcttccggc gatgattatc ggggcgttta ttatcgacct gctgttactg
     4081 gccctgacat tatttgtcgg ccaggtatta agcgacaacc tgaatgcagg cagtcgcacc
     4141 atcgctttcc aacaaagttt gtttctcaac gcctttgccc tgattgaatt tttcaaagcc
     4201 gtactacgcc tgattttttg cccaaacgtg gcggagctgc gcccgttcac gattcaggac
     4261 gagagcgccc gttactggag tcgtcgcctg agctggttaa gcagtttgat aggttatggc
     4321 ctgattgtgg ccgtgccgat tatctctaat caggtgaatg tacagatagg tgcgctggcg
     4381 aacgtcatca ttatgctgtg catgaccgtc tgggcgttgt acctgatctt tcgtaataaa
     4441 aaagagatta cccagcattt gctcaacttt gcggagcatt cgctggcctt tttcagcctg
     4501 tttatccgcg cctttgcgct ggtgtggcac tggctggcaa gcgcctattt tatcgtgctg
     4561 tttttctttt cgttgttcga tccgggcaac agcctgaaat ttatgatggg tgcaacggtg
     4621 cgcagcctgg cgattattgg tatcgcagcg tttgtttccg gtatgttttc ccgctggctg
     4681 gcaaaaacca tcactctctc gccacatact cagcgtaact atccagagtt gcaaaaacgg
     4741 ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggcg
     4801 gtaatgttgc tgttgagcgc atggggattg ttcgatttct ggaactggct gcaaaacggc
     4861 gcggggcaga aaaccgtaga tatcctgatc cgtatcgcac ttattctttt tttctcggcg
     4921 gttggctgga cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga tattcatggc
     4981 cgcccgctac ccagcgcccg tacgcgtacc ctgctgacgc tgttccgtaa cgcgctggcg
     5041 gtgattatca gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg
     5101 ccattgctgg caggtgccgg ggcattaggt ctggctatct cgtttggttc gcaaacgctg
     5161 gtgaaagata tcattaccgg ggtgtttatt cagtttgaaa acggcatgaa cactggagat
     5221 ttggtgacta tcgggccgtt gaccggcaca gtggaacgga tgtcgattcg ctccgtgggc
     5281 gtgcgccagg ataccggggc gtatcacatc attccgtggt cttcgataac cacctttgct
     5341 aacttcgtcc gcggtattgg ttcggtagtg gcaaactacg atgttgatcg ccatgaagat
     5401 gctgataaag ccaatcaggc actgaaagat gcggtagcgg aattaatgga aaacgaagaa
     5461 attcgcgggc tgattattgg tgaaccgaat tttgccggga ttgtcggctt aagcaatacc
     5521 gcgtttacac tgcgtgtttc gttcaccacg ctgccactca aacagtggac ggtacgcttt
     5581 gccctcgaca gccaggtgaa aaaacatttc gacctggcgg gcgttcgcgc gccagtgcag
     5641 acttatcagg tgctgcctac tccgggcgcg accccggctg aaccgttacc gccgggggag
     5701 ccaacgcttt aacgctggcg attgacaaaa cggcggcgct gctcgtcgtt cataaaggtc
     5761 caggcaataa agcgactctg cttttgcccc tgggccatct ctttttttac caccttcacc
     5821 gcgcccacgt cggtcagagc acgatacaac ggcggtaagt tttcaccacg agataccagc
     5881 gaggtaaacc acatcacctg cttcgcgaag cctttgctct cttcaatcat ctttttgata
     5941 aaggcgactt caccaccttc acaccacaac tcctgttgct gaccaccaaa gttcagtgca
     6001 tcgtctttgt tcagccccag gttacggcgt ttacgctcac tacctgcccg cgctgcagcg
     6061 gcggaatcgt ggaatggcgg gttacacaag gtcgcgtcgt attgctcgtt tttatggatg
     6121 atgccgttaa aaattgcccc actctctttt tgccgacgca gacgaatggc gcggttaagc
     6181 cccggattag cactgataat cgcctgcgca ctgcttaacg cctggctgct ggtttcacta
     6241 ccggtaaaac gccagccata ttcatgtacg ccaatcagcg gataaataca gttcgcacca
     6301 acgccgatat ccagaatgct ggcattcgct ggaattgttc cgcttgcttc agccagtaaa
     6361 tcggcaaggt gatgaatata atccgcccgg cccggtactg gtgggcagag aaaaccgtcg
     6421 gggatatccc agttcgctac ggcgtaaaaa tgggccagca acgccttatt gagcgccttc
     6481 accgccagcg gattggcaaa gtctacgctt tgctccccgg cgggtgtaag cgtgaggaat
     6541 tgcctgagtt caggattgac ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg
     6601 ttgcgcggat gcaaccccgg tttctgggcg gacatagcat tctcctttaa gcagcggcgt
     6661 aagatacccg cataacgcga cagggtaaat atctggctgc gtttatcatt ccccaaaacc
     6721 ttaacatggc tatggtttat ggcaattcat cataaaaatc agcattatgc atgtcattaa
     6781 tttgtcttat tttgtaaaaa tatatcgttc aaaaaataag cacatctctg tcaatttttt
     6841 gcgtcatttg catcgcgcag gcgacctaca cttatcaggc actacccgtt gtatttatga
     6901 ggatgtcatt atgaaaaagt gcctcacact actgattgcc accgtcctga gcggaatctc
     6961 tctcacggct tatgccgcgc aaccgatgag taacctggac agcggtcagc tacggcccgc
     7021 cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat ctggaggata aactggcaga
     7081 aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat tccgcaggcg gaaatgacca
     7141 gatgttcggt actgcaacca tctacaaata accccaccaa cgtaactgca ttcgcctctg
     7201 gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa tatgaactca atgtaaataa
     7261 atgtatttct ttttcgcgca atgggtgata gaaaatcgcg ccaagtgata atgcttatca
     7321 aaattattat cacttgcacg aacactatca cgggattaac agtggcatcg catccgcaga
     7381 gatgctttct cgtggcagtg aaaatttcaa catataagaa aaagtcacct gcaaaatgga
     7441 aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttctttg ccggtctttg
     7501 tattggcatc acgcctgtgg ctcaggcact cgccgccgaa gggcaaacta acgcggatga
     7561 cacgctggtt gtcgaagcat caacgccttc gctttatgcg ccacaacaat ctgccgatcc
     7621 gaaattctcg cgtccggtag cggatactac ccgcacgatg acggtgattt ctgaacaagt
     7681 gattaaagat cagggcgcaa ccaaccttac cgacgcgctc aaaaacgccc ccggcg
//
LOCUS       DAZRUF010000193         7714 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZRUF010000193 DAZRUF010000000
VERSION     DAZRUF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.194"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..642
                     /locus_tag="ACHXOL_003328"
     CDS             <1..642
                     /locus_tag="ACHXOL_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR6810236.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            893..2455
                     /gene="hpaB"
                     /locus_tag="ACHXOL_003329"
     CDS             893..2455
                     /gene="hpaB"
                     /locus_tag="ACHXOL_003329"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR6810237.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2473..2985
                     /locus_tag="ACHXOL_003330"
     CDS             2473..2985
                     /locus_tag="ACHXOL_003330"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR6810238.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3378..5528
                     /gene="btsT"
                     /locus_tag="ACHXOL_003331"
     CDS             3378..5528
                     /gene="btsT"
                     /locus_tag="ACHXOL_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR6810239.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5578..5781
                     /locus_tag="ACHXOL_003332"
     CDS             5578..5781
                     /locus_tag="ACHXOL_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR6810240.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5792..6748
                     /gene="yjiA"
                     /locus_tag="ACHXOL_003333"
     CDS             5792..6748
                     /gene="yjiA"
                     /locus_tag="ACHXOL_003333"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR6810241.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6826..>7714)
                     /locus_tag="ACHXOL_003334"
     CDS             complement(6826..>7714)
                     /locus_tag="ACHXOL_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR6810242.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1782 a         2103 c         2022 g         1807 t
ORIGIN      
        1 catgcgttta ttacggagtc tgacgccgac ggtcatgtgt tgacggtacg ggaagatctg
       61 atctggcccc tgctggaagt tctttatcca ggcactcggg aaaccttcgg cctgccgggg
      121 atttgcctgt cactggcaga taaacccgac gaactggcgg cgctggaaca ctattggcaa
      181 ctgatagagc gggaatcggt ggaacaactg cctggacggg aacacaccct gacgctactg
      241 gcacaggcag tgttcaccct actgctgcgt aatgcaaaac tcgacgacca tgccgccagc
      301 gggatgcgcg gagaattaaa actgttccag cgttttaata tgctgattga aagccatttt
      361 catcagcact ggacagtacc ggattacgct aacgaactgc atatcaccga atcacgcctc
      421 acggacatct gccgccgctt tgccaaccgt ccgccaaaac ggttgatttt cgacaggcag
      481 ctgcgagaag ccaagcggct gctgctgttt tctgataacg ccgtgaacaa tattgcctgg
      541 caactcggtt ttaaggatcc agcttatttt gcgcgctttt ttaatcgctt agtcggttgc
      601 tcgcccagtg cttatcgtgc caaaaaagta cctgtgacgt gaaaatccct tagccttaac
      661 gggaaaccag gcaccacctg ctattcccct ttctccgcct ggagcaaggg gatttagtaa
      721 cgtcaaaaat cgctaaaagc gaccgcgatc acaaaaccgt acccgacctg aaaagtacca
      781 gccattgtcc caaaagtgtc ttcccgtaat cgcccctgca agcttcaatt gaataacaaa
      841 aacaacacac aaaattaaca atcgtattcc gattaatact gtagaggtcg acatgaaacc
      901 agaagatttc cgcgccagta cccaacgtcc gttcaccggg gaagagtatc tgaaaagcct
      961 gcaggatggt cgcgagatct atatctatgg cgagcgagtg aaagacgtca ctactcatcc
     1021 ggcatttcgt aatgcggcag cgtctgttgc ccaactgtac gacgcgctgc acaaaccgga
     1081 gatgcaggac tctctgtgct ggaacaccga caccggcagc ggcggctata cccataaatt
     1141 cttccgcgtg gcgaaaagtg ccgacgacct gcgccagcaa cgtgacgcca tcgctgagtg
     1201 gtcacgcctg agctatggct ggatgggccg taccccagac tacaaagctg ctttcggttg
     1261 cgcactgggc gcgaatccgg gcttttacgg tcagttcgag cagaacgccc gtaactggta
     1321 cacccgtatt caggaaactg gcctctactt taaccacgcg attgttaacc caccgatcga
     1381 tcgtcatttg ccgaccgata aagtgaaaga cgtttacatc aagctggaaa aagagactga
     1441 cgccgggatt atcgttagcg gtgcgaaagt ggttgccacc aactcggcgc tgactcacta
     1501 caacatgatt ggcttcggct cggcacaagt aatgggcgaa aacccggact tcgcactgat
     1561 gttcgttgcg ccaatggatg ccaatggcgt caaattaatc tcccgcgcct cttatgagat
     1621 ggtcgcgggt gctaccggct cgccgtacga ctacccgctc tccagccgct tcgatgagaa
     1681 cgatgcgatt ctggtgatgg ataacgtgct gatcccatgg gaaaacgtac tgatctaccg
     1741 cgatttcgat cgctgccgtc gctggacaat ggaaggcggt tttgcccgta tgtatccgct
     1801 gcaagcctgt gtgcgcctgg cagtgaaact cgacttcatt acagcactgc tgaaaaaatc
     1861 actcgaatgt accggcaccc tggagttccg tggtgtacag gccgatctcg gtgaagtggt
     1921 ggcgtggcgc aacaccttct gggcattgag tgactcgatg tgttctgaag cgacgccgtg
     1981 ggtcaacggg gcttatttac cggatcatgc cgcactgcaa acctatcgcg tactggcacc
     2041 aatggcctac gcgaagatca aaaacattat cgaacgcaac gttaccagtg gcctgatcta
     2101 tctcccttcc agtgcccgtg acctgaacaa tccgcagatc gaccagtatc tggcgaagta
     2161 tgtgcgcggt tcgaatggta tggatcacgt ccagcgcatc aagatcctca aactgatgtg
     2221 ggacgccatt ggcagcgagt ttggtggtcg ccacgaactg tatgaaatca actactccgg
     2281 tagccaggat gagattcgcc tacagtgtct gcgccaggca caaagctccg gcaatatgga
     2341 caagatgatg gcgatggttg atcgctgcct gtcggaatac gaccagaacg gctggactgt
     2401 gccgcacttg cacaacaacg acgatatcaa catgctggat aagctgctga aataacgcag
     2461 caggaggtta agatgcaatt agatgaacag cgcctgcgct ttcgtgacgc gatggccagc
     2521 ctgtcggcag cggtaaatat tatcaccacc gagggcgacg ccggacaatg cgggattacg
     2581 gcaacggccg tctgctcggt cacggataca ccaccatcgc tgatggtgtg cattaacgcc
     2641 aacagtgcga tgaacccggt ttttcagggc aacggtaagt tgtgcgtcaa cgtcctcaac
     2701 catgagcagg aactgatggc acgccacttc gcgggcatga caggcatggc gatggaagag
     2761 cgttttagcc tctcatgctg gcaaaaaggt ccgctggcgc agccggtgct gaaaggttcg
     2821 ctggccagtc ttgaaggtga gatccgcgat gtgcaggcaa ttggcacaca tctggtgtat
     2881 ctggtggaga ttaaaaacat catcctcagt gcagaaggtc acggacttat ctactttaaa
     2941 cgccgtttcc atccggtgat gctggaaatg gaagctgcga tttaagctcc agccccttca
     3001 cgcattcgtc gaaggggcat ttttattgtg atctcaatca catatccacc cgatgaataa
     3061 ttcttgccca accgctcacc gccattcctg accgcttacg tccctaaaat aaccactcag
     3121 ttatttacct tactttacgc gcgcgtaact ctggcaacat cactgcagga tagcggtcaa
     3181 tttacctcct caaacgcaac gcaaacctag aacggcttcg gcctatgatt aacaaattac
     3241 tctatgaatt ttggtttgca ggaaggcagc aagtgagtga atccccggga gcttacaaca
     3301 gtaagtgaca ggggtgaacg aacgcaactg ccgcacctgt aagccaaaag acgacgagta
     3361 aaatgccagg ttttactatg gatacgaaaa aactattcaa gcacataccc tgggtgattc
     3421 tcggaatcat cggtgcattc tgcctcgcgg tagttgcatt acgtcggggg gagcacgtca
     3481 gcgccctgtg gatcgtggtc gcctctgtgt cggtgtatct ggtggcgtat cgctactaca
     3541 gtctgtacat cgcccagaag gtgatgaaac tcgaccccac gcgcgcgacg cctgcggtta
     3601 ttaacaacga cggtctgaac tacgttccga ccaaccgtta cgtgttgttt ggtcaccact
     3661 tcgccgctat cgccggtgct ggtccgctgg tcggtccggt tctcgccgca cagatgggcc
     3721 acctgcctgg cacgctgtgg ctgctggcgg gggtagtact ggccggtgcg gttcaggact
     3781 ttatggtgct gtttatctcc tctcgccgta acggcgcatc tctcggtgag atgataaaag
     3841 aagagatggg accagtaccg gggactatcg cgctgtttgg ctgtttctta atcatgatca
     3901 tcatccttgc cgtcctggcg ctgattgtgg ttaaagccct ggccgaaagt ccgtggggtg
     3961 tcttcaccgt ttgctcaacc gtaccgattg cgctgtttat gggtatctac atgcgcttta
     4021 tccgtccggg gcgtgtgggt gaagtctctg tcattggtat cgtgctgctg gttgcctcta
     4081 tctacttcgg tggcgtgatt gctcacgatc cgtactgggg tccggcactg acctttaaag
     4141 acaccaccat taccttcgcg ctgattggct atgcgtttgt ttccgcattg ctgccggtgt
     4201 ggctgatcct cgcaccgcgc gactatctgg caaccttcct gaaaatcggc gttatcgtcg
     4261 gcctggcgct gggtatcgtg gtgctgaacc cggaactgaa aatgcctgcg atgacccagt
     4321 acattgacgg tactggcccg ctgtggaaag gtgctctgtt cccgttcttg ttcattacca
     4381 tcgcctgtgg tgcggtatct ggcttccacg cactgatctc ttccggtacg acgccgaaac
     4441 tgctggctaa tgaaaccgac gcgcgtttca ttggctacgg cgcaatgctg atggagtcct
     4501 tcgtggcgat tatggcgctg gttgctgcgt ccatcatcga accgggtctt tacttcgcga
     4561 tgaacacccc gcctgctggc cttggcatca ccatgcctaa cctgcatgaa atgggtggcg
     4621 agaacgcgcc gatcatcatg gcgcagctga aagacgttac cgcacacgcg gcagcgaccg
     4681 tcagctcttg gggcttcgtg atttcgccag agcagatcct gcaaaccgcg aaagacattg
     4741 gtgagccttc tgtcctgaac cgtgcaggtg gtgcgccaac gctggcggta ggtatcgctc
     4801 acgtgttcca caaagtgctg ccgatggctg acatgggctt ctggtatcac ttcggtattc
     4861 tgttcgaagc cctgttcatc ctgaccgcgc tggatgcggg tacccgttct ggccgtttta
     4921 tgctgcaaga cctgctgggt aacttcatcc cgttcctgaa aaaaaccgac tctctggttg
     4981 ccggtatcat cggtactgcg ggctgtgtgg gtctgtgggg ctacctgctg tatcagggcg
     5041 tggtcgatcc gctgggcggc gttaagagcc tgtggccgct gttcggtatc tccaaccaga
     5101 tgctggcagc cgtagcgctg gtactgggca ccgttgtgct gattaagatg aagcgcaccc
     5161 aatacatctg ggtaactgtt gttccggctg tatggctgct tatctgcacc acctgggcgc
     5221 tgggtctgaa actgttcagc accaacccgc agatggaagg cttcttctac atggcaagcc
     5281 agtacaaaga gaagattgct aacggtactg acctgacggc gcagcagatt gccaacatga
     5341 accacatcgt tgtgaacaac tacaccaacg caggcctgag tattctgttc ctgattgtgg
     5401 tgtacagcat catcttctac ggtttcaaaa cctggcttgc ggtgcgtaac agcgacaaac
     5461 gtactgacaa agaaactccg tacgttccaa tcccggaagg cggcgtgaag atctcttcgc
     5521 accactaacc gtgtttagcc ccgcttcggc ggggctttgt tcttatcaga gtgaactatg
     5581 tttggtaact taggacaggc aaaaaaatat ctcggccagg cggcgaagat gttgattggc
     5641 attccagact acgacaacta cgtcgagcat atgaagacca accatcccga caagccgtac
     5701 atgagctatg aagaattctt ccgcgaacgc cagaacgcgc gctacggcgg cgatggtaaa
     5761 ggcggtatgc gctgttgtta atggagagag catgaacccg attgcagtta ccctactcac
     5821 cggtttttta ggcgcgggca aaaccaccct gctgcgccat attcttaacg aacaacacgg
     5881 ctacgagatt gccgtgattg aaaacgaatt cggcgaagtc tccgttgatg atcaactgat
     5941 tggcgaccgc gccacgcaga tcaaaacgct gaccaacggc tgcatctgct gttcgcgctc
     6001 caacgagctg gaagacgcac tactcgacct gctggataat ctcgacaagg gcaatattca
     6061 gttcgaccgt ctggtcattg aatgcaccgg catggccgat cccggcccga ttattcagac
     6121 cttcttctcc catgagattt tatgccagcg ctacctgctg gacggggtga ttgcgctggt
     6181 ggatgcggta catgccgatg agcagatgaa ccagttcacc atcgcccagt cccaggttgg
     6241 ctacgccgac cgtattctgc tgacgaaaac cgacgtcgca ggcgaagcag agaaactgcg
     6301 tgaacgcctg gcgcgcatca acgcccgcgc accggtctac accgttaccc acggcgatat
     6361 cgacctgggc cttctgttca acaccaacgg ttttatgctg gaagaaaacg tcgtcagcac
     6421 caaaccgcgt ttccacttta tcgcggacaa acaaaacgat atttcgtcga ttgtggtgga
     6481 actggattac ccggtagata tcagcgaagt ttcccgggtg atggaaaacc tgctgctgga
     6541 atcggcggat aaactgctgc gttacaaagg gatgctgtgg attgacggcg aacctaaccg
     6601 cctgctgttc cagggcgtcc agcgcctcta cagcgccgac tgggaccgcc cgtggggcga
     6661 tgaaaaaccg catagcacga tggtgtttat cgggattcag ttgccggagg atgagattcg
     6721 ggcggcgttt gcgggactca aaaagtaacc agccctccaa catcacaata cctgagactt
     6781 tatcaccctc atattcctcc agaatatgag ggcattcggg cttaattatc tctgaacgtg
     6841 caacacttcc tgcgactcct tatgcgcctc cacatcctgc ttcgttaacc agagcgactt
     6901 acgaccaaaa ttttcgtaca ttttcagctg atcttcatag tgctgatcaa ctgttccatc
     6961 gggagcaata aacccactct gaccgggtgc gaccacatcc caggcaagca ccggacgatc
     7021 gcttgtcgtt ggtgagaaaa caatcatatc gttttctgtt ccacggtttt gatactccgc
     7081 ctgatgacgc gtttcttccg ctgcggcctg cggtacacca aagaaattat ttgcacggaa
     7141 cgttaaggcc attgcaggtg ttttccagtt actcacatta ttgccatagc gtttggaaag
     7201 agtctcccag gtatcttcca gcgcagccaa cacaacctcc tgctgtggtt tcccagcaaa
     7261 cagatcaacc gcctgtggga ttggtgattt gtctccctgc accgcctcat acaaaatttt
     7321 tgctccaaca cttatattca gcgaaccagt tgggccgtcc tgggttgttt cgtagccact
     7381 ggcgctgtac cacttatcaa atggcatagg tacggcagcc actacggtac gcttcaacat
     7441 actggtcagc caaacgttca ggatggcaga gcctggctgc tgccaggttt taccatcatc
     7501 attaagcaaa ttgatgccat cccaacgtgt taatgtatcc accaactgac ggcgcggatc
     7561 gctctgtgtc aaaccagatg tcgctgcttg cagagtaggt aaaaaaagcc tcaggttaag
     7621 atcctgacga ctggtttggc gaataacatc ccatgcctga tcagcagtta agcgtggctt
     7681 ttgctcaagc agtcggtcga tctccgtaac gcga
//
LOCUS       DAZRUF010000194         7683 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZRUF010000194 DAZRUF010000000
VERSION     DAZRUF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.195"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(224..970)
                     /gene="cutC"
                     /locus_tag="ACHXOL_003335"
     CDS             complement(224..970)
                     /gene="cutC"
                     /locus_tag="ACHXOL_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR6810243.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(984..1550)
                     /locus_tag="ACHXOL_003336"
     CDS             complement(984..1550)
                     /locus_tag="ACHXOL_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR6810244.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1766..3502
                     /gene="argS"
                     /locus_tag="ACHXOL_003337"
     CDS             1766..3502
                     /gene="argS"
                     /locus_tag="ACHXOL_003337"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR6810245.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(3544..3588)
                     /gene="yecV"
                     /locus_tag="ACHXOL_003338"
     CDS             complement(3544..3588)
                     /gene="yecV"
                     /locus_tag="ACHXOL_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR6810246.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3679..4167
                     /locus_tag="ACHXOL_003339"
     CDS             3679..4167
                     /locus_tag="ACHXOL_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR6810247.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIENDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4287..4679)
                     /gene="flhe"
                     /locus_tag="ACHXOL_003340"
     CDS             complement(4287..4679)
                     /gene="flhe"
                     /locus_tag="ACHXOL_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR6810248.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4679..6757)
                     /gene="flhA"
                     /locus_tag="ACHXOL_003341"
     CDS             complement(4679..6757)
                     /gene="flhA"
                     /locus_tag="ACHXOL_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFR6810249.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6750..>7683)
                     /gene="flhB"
                     /locus_tag="ACHXOL_003342"
     CDS             complement(6750..>7683)
                     /gene="flhB"
                     /locus_tag="ACHXOL_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR6810250.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1961 a         2075 c         1872 g         1775 t
ORIGIN      
        1 tttagaatat atatttatca gttttagcaa tttaaatccc ataatcaatg tgaatataaa
       61 aaatattatt agcacaacgc tatgttcact gtcacccgct caacagatca cggttatcgt
      121 tcgtttttta tactgctcag ggataaaaaa aggcccctgt tgaaattgca ggggcctggt
      181 acgagcaagc atcatattgg gcgacatgat gcaacggtaa aaatcatttg gcctgatggc
      241 gttcaatgat tcctttcatt tcagcaaccg ccgccccgtc tacgatataa cgcgaatact
      301 cgtccgcgtg ttcatctgat gacatggaca atccttgatt acgataacgc atcggtgagg
      361 cttgccacgc tcccgcggag ctatggactt ccagcactcc ggcatcgagg aagtggtgca
      421 ggttttctgc acggactcct gctccggcca taatgattgg agcatcacga tgggcaataa
      481 gttccataat ttttgataaa ccttgcagtg cgtctgattt ttgccctgat gtcagtaccc
      541 gggcaatgcc caattccgca agattattga gtgtatataa agggttagcg cacatatcga
      601 aggcgcgatg aaaagtcact gccagcggac cggcagcagc cattattttt tccattcgtg
      661 gcatatcgac attcccgtca acatcgagaa cgcccgtcac cagtccagga aaacctaatt
      721 cgcggaccgt gcgcacatcc tcaagaatgg cggcaaactc accgtcgctg tagcaaaaat
      781 caccaccgcg tgggcgaatt atcggatgca cagggatcgt cacccgctgg cgcacggatt
      841 tcagtacccc caacgacggc gttaagcccc cctcttttgg ggctgcgcat aattcaactc
      901 tgtctgcgcc gttttgctgc gccgttagtg cacattccat gctgtaacag caaatttcca
      961 gtaatgccat ttttgctcct taattacgct gactgctcgc tggcaacgat ctcttcaatg
     1021 gaccacggat gaaacttaat agttgttttg ccatcggtga ccgccagcgt cggattcggt
     1081 aatcgctcat gctcaccttt tggtgaactg gttttaactg agatccccgg taggctgagt
     1141 ccttcatcag aaagcaatgc cagcgcacgg gcattcagcg tttctggatc acccagcaga
     1201 acgatttcaa tatgttccca accttcgtgt gggtaacgtt tttccccggg ccacggtagc
     1261 tccacaatag aaaactgcca gtgcgcaacc tgtaccggtt catgcaattt aaacagacaa
     1321 atcggtctgc cattgatcat attttctgac aaaagctcgc cacactgttc aaacccgcga
     1381 cgccagcgtt cagcagtggc gttttgatgg caacgcaaag aaatgtgatc ggcagtcagc
     1441 ggagtgatat tcaaccccag acggagggaa agttcatcta atgcgtgggt aaatcgcggt
     1501 aaatccgatg caatatcctg cagctcgtcg atagattgcc agttcgccat aatcactctt
     1561 cgtctttcag taaaagcgtt aatttaccct gttgccctgc gccaaccaac cgctgatttc
     1621 acgccgcttc tgatgcaata gtgaaaacgg caatacgcca cgcgcacgtt gctgacgaaa
     1681 acagccattt gcagtatact cccgccctaa tttctttaac tggtgcgggc aatttttgct
     1741 cgcttcatca atgtaaggta ttccggtgaa tattcaggct cttctctcag aaaaagtccg
     1801 tcaggccatg attgcggcag gcgcgcctgc ggattgcgaa ccgcaggttc gtcattcagc
     1861 aaaagttcag ttcggcgact atcaggctaa cggcatgatg gcagttgcta aaaaactggg
     1921 tatgacaccg cgacaattag cagagcaggt gctgactcat ctggatctta acggtatcgc
     1981 cagcaaagtt gagatcgccg gtccaggctt tatcaacatt ttccttgatc cggcattcct
     2041 ggctgaacat gttcagcagg cgctggcgtc cgatcgtctc ggtgtttcta cgccagaaaa
     2101 acagaccatt gtggttgact actctgcgcc aaacgtggcg aaagagatgc atgtcggtca
     2161 cctgcgctct accattattg gtgacgcagc agtgcgtact ctggagttcc tcggtcacaa
     2221 agtgattcgc gcaaaccacg tcggcgactg gggcactcag ttcggtatgc tgattgcatg
     2281 gctggaaaag cagcagcagg aaaacgccgg tgatgaaatg gagctggctg accttgaagg
     2341 tttctaccgc gatgcgaaaa agcattacga cgaagatgaa gagtttgccg agcgtgcacg
     2401 taactacgtg gtaaaactgc aaagcggtga cgaatatttc cgcgagatgt ggcgcaaact
     2461 ggtcgacatc accatgacgc agaaccagat cacctacgat cgtctcaacg tgacgctgac
     2521 ccgtgatgac gtgatgggcg aaagcctcta caacccaatg ctgccaggaa ttgtggcgga
     2581 tctcaaagcc aaaggtctgg cagtagaaag cgaaggggcg accgtcgtat tccttgatga
     2641 gtttaaaaac aaggaaggcg aaccgatggg cgtgatcatt cagaagaaag atggcggcta
     2701 tctctacacc accactgata tcgcctgtgc gaaatatcgt tatgaaacac tgcatgccga
     2761 tcgcgtgctg tattacatcg actcccgtca gcatcaacac ctgatgcagg catgggcgat
     2821 cgtccgtaaa gcaggctatg taccggaatc cgtaccgctg gaacaccaca tgttcggcat
     2881 gatgctgggt aaagacggca aaccgttcaa aacccgcgcg ggtggtacag tgaaactggc
     2941 cgatctgctg gatgaagccc tggaacgtgc acgccgtctg gtggcagaaa agaacccgga
     3001 tatgccagct gacgagctgg aaaaactggc taacgcggtt ggtattggtg cggtgaaata
     3061 tgcggatctc tccaaaaacc gcaccacgga ctacatcttc gactgggaca acatgctggc
     3121 gtttgagggt aataccgcgc catacatgca gtatgcttac acgcgtgtat tgtccgtgtt
     3181 ccgtaaggca gaaattaacg aagagcaact ggctgcagct ccggtaatca tccgtgaaga
     3241 tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc
     3301 ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac gatctggctg gtctgttctc
     3361 tggcttctac gagcactgcc cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg
     3421 tctgaaactg gcacaactga cagcgaagac gctaaagctg ggtctggata cgctgggtat
     3481 tgagacagta gagcgtatgt aatcgatttt tcgtgagagt aaagcctgat cagggttacc
     3541 tgatcaggct tttttattgc catctaaatg tattctgaaa atggacatgc cattgttttc
     3601 tgactgttgg ataagaggcc agaagcgtaa tatccggccc cagggaaacg ataacggttg
     3661 aatttaagga ataccgcagt gtttaaattt cttgtattaa cattaggcat tatctcttgc
     3721 caggcttacg cagaagatac ggttatagaa aacgaccatg acatttcagc catcaaagat
     3781 tgttggcaaa aaaattcaga tgatgatact gacattaacg tgatcaaatc atgcctgcga
     3841 caagaataca atctcgtcga tgcgcaatta aataaagcct atggtgaagc ttatcgttat
     3901 atagaacaag tgccacgcac aggtgtaaaa aaacctgata ccgaacaact taacttgctt
     3961 aaaaaatcac agcgagcctg gctggatttt agggacaaag aatgtgaatt aatcctttca
     4021 aatgaggacg ttcaggattt aagtgaccct tattctgaat cagaatggct ctcatgtatg
     4081 atcatacaga ccaatacgcg aactcgccag ttgcagctat accgtaactc tgaagatttt
     4141 tatccaagcc ctttgacaag aggataattc acatcttttt ggcatgtttt gttgcaagct
     4201 attcctgata aataattgca acaagacatc gagccttttt cactgagtta ttaaacatac
     4261 tcgcgagcgc gtaatttttt tgtccttcag cgataattca caatcacttc attacgttgt
     4321 acctttagcg gtggaattaa ccgcccaccg cctggcactt cccagataaa tcgcaacggt
     4381 tctgctgccg ctataccgga aaaggccacg gtggttccgc tctgcccctc taattcgaca
     4441 caacgagatt gcgaacacaa gcgaacccgc agtcctgaag gtgtcgggcc gataagctga
     4501 taacgccacg ctaccagcgt catcaatcct gaagcaggtt gtcgcgtaga aagaggcgca
     4561 gacgacatcg actcaccgcg atgatttagc gtaattccta cactacttgc ctgccacatc
     4621 ccctccccgg cggcttgcac cagcagcgga aacaataata ttgctaataa ggttctcatt
     4681 atttgccgcc aattgtcgcc gtcatgcgga tatgtcggtt atcagacagt tccagattcg
     4741 acaggaccac taactgcggc aagctgcggc gcaggaagcg agacaataat ggtcgcagcg
     4801 cgtggttcac caacaatact ggcggcgcgc ccagcatctc ctgacgggat agcgcttcct
     4861 gagtttgcgc cagtaaacga tccgccagcc ctggctccag tcctcccccg ccctgcagcg
     4921 cctgtagtaa caaacgttcc agcggtgtat cgaggccaat aacatggact tcatctttgc
     4981 caggaaacca ctgctgggta atcgcccgtc ccaacgccac gcgcacgacg gcggttaatt
     5041 catgtggatc gctttggatg ggcgcatgtt ccgccagcgt ttcgagaatg gtgcgcatat
     5101 cgcgaatcgg tactttttca tcgaggagat tttgcagcac tttatgcagt gtggtgagcg
     5161 tgacgacgcc aggaacgaga tcttccgtca gctttggcat ctcctgggcg acgcgatcca
     5221 acagctgttg cgcctcctga cgaccaaaca gctctgcggc atgctggcta atgaggtggt
     5281 taagatgcgt tgctaccacc gtgctggcct caaccactgt gtacccctga atctgcgcct
     5341 gttcttttag cgcactttca atccagatag cattcaggcc aaatgccgga tcgacggtcg
     5401 cctcaccagg taacgtcccg gcagcggttc cagggttaat cgccagccag cgccccggat
     5461 aagcatcacc actgccaatc tccacgcctt tcatcaaaat gcgatagcgg gcaggttgca
     5521 gatccatatt gtcgcgaatg tgcaccactg gcggcagaaa tcccatctcc tgggcaaatt
     5581 tcttgcggat actgcgtata cggcccaaca actcaccatc ctgctggaaa tcgaccatcg
     5641 ggatcagtcg ataacccact tccattccca gagaatcttc cagttgtaca tcgttccacg
     5701 tcgcttcgac aacggtatta ttctctgcca tttttaccgg tttgggttcg gcaggcgctt
     5761 tttgttcgcg tccgcgtatc caccaggcca gcccgagcaa tccggcagtg aacagcaaaa
     5821 ataccaggtt cggcattcca ggcaccaggc cgagtaaacc gagcacggcg gcgcttaaca
     5881 acataacgct tgggttactg aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg
     5941 tgctgacacg cgtaacgatg acccccgcgg cggtagaaat caccagcgcc ggaatttgtg
     6001 ccaccagacc gtcgccaatg gtcaatagcg tataactttc cgccgcgtgt cccatgctca
     6061 tgccatgttg cagcacgccg accagcaacc cgccgacaac gttaatgacc atgatgagga
     6121 tcccggcgat ggcatcgccg cgaacaaact tacttgcccc gtccattgag ccgtaaaaat
     6181 cggcttcctg agtcacttcg gagcggcgtt ttttcgcctc atcttcacca atcaatccag
     6241 cgttaaggtc ggcgtcaatc gccatctgct tacccggcat accatcgaga acaaagcgcg
     6301 cacccacttc tgcgatacgc ccggcacctt tggtaatgac cataaagttg atgatcacga
     6361 gaatgacaaa caccacgata ccgatagcga aattgccacc aacgaggaag tgaccgaacg
     6421 cttcgaccac cttccctgcc gccgccgcgc cggtatgccc ttccatcaaa atgatacggg
     6481 ttgaagccac gttaagtgcc agacgcaaca acgtagtaaa caacagaatg gtcggaaacg
     6541 cagcaaactc aagcgtgcgc tgggtaaaca tcgccaccag caacaccatg atcgacaagg
     6601 caatattgaa ggtaaacaac aggtcgagta tgaatgcggg cagtggcagc accatcatcg
     6661 acaagatcaa caggatcaaa atcggtccgg caaggatctg ccattgtgtc gatttcaggt
     6721 ttgcgggcag gcgcagcatc gcggccagat tactcatggg tcggtttctc gttaataaaa
     6781 tccagggctt ccggcaccgg aagatgagta ggttgtacag ggcgctgtcc accagccaga
     6841 cgccagcgtt tcagttgcca gacccaggcc agcacttccg ccaccgcggc gtacagttga
     6901 cccgggattt gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct
     6961 tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct
     7021 gcacctttag cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc
     7081 gaatagtggg tcgggttatt gacaatgaca tcagctttcg gcacatcggc catcatccga
     7141 cgccgtgcag cagctcgctg catctgacgg atccgccctt taacatgagg gtcaccttcg
     7201 ctttgtttga actcatcacg aatatcctgc cgcgacatac gcagcttttt caggtggctg
     7261 aagatttgga aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagt
     7321 gcgcatagcc caaccagatc catcgcatta cccatggcgg tagtcggaga ctcggccatt
     7381 aagcgcatca tctgcggcca gtgatgccag agataaaacc ccgtcacgct gccaaccagg
     7441 atggttttca aaattgcctt aagcaactcc gcgccagtct gtgccgagaa catccgttta
     7501 atgcccggta gcgggttgag tttggaaaac ttcggctgca aggatttgcc gctaaatacc
     7561 agccccccca gcatgaccgg agaaatgagc gccaccagca ccacgccgct aatcagcggc
     7621 agcagcgcca gcatggcttc tctgatcagc agaataatct gcccgaggat cagattcggg
     7681 tca
//
LOCUS       DAZRUF010000195         7618 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZRUF010000195 DAZRUF010000000
VERSION     DAZRUF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.196"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(148..699)
                     /locus_tag="ACHXOL_003343"
     CDS             complement(148..699)
                     /locus_tag="ACHXOL_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR6810251.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            1286..1633
                     /locus_tag="ACHXOL_003344"
                     /pseudo
     CDS             1286..1633
                     /locus_tag="ACHXOL_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            1686..2081
                     /locus_tag="ACHXOL_003345"
     CDS             1686..2081
                     /locus_tag="ACHXOL_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR6810252.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2122..2865
                     /gene="cmoA"
                     /locus_tag="ACHXOL_003346"
     CDS             2122..2865
                     /gene="cmoA"
                     /locus_tag="ACHXOL_003346"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR6810253.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2862..3833
                     /gene="cmoB"
                     /locus_tag="ACHXOL_003347"
     CDS             2862..3833
                     /gene="cmoB"
                     /locus_tag="ACHXOL_003347"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR6810254.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3998..6427)
                     /gene="torZ"
                     /locus_tag="ACHXOL_003348"
     CDS             complement(3998..6427)
                     /gene="torZ"
                     /locus_tag="ACHXOL_003348"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR6810255.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKDGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6452..7552)
                     /locus_tag="ACHXOL_003349"
     CDS             complement(6452..7552)
                     /locus_tag="ACHXOL_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR6810256.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         1805 a         1951 c         1739 g         2123 t
ORIGIN      
        1 tccccgaatc ttcgaaaata tctgtctagt tgattcggta gaatctacct catgatgtat
       61 acgtgtttag tacccgtcac accagctatg tgaccccctt ggcaaaaaag ccaaatatca
      121 gctacatcca tgtaactatt cagtaactta tttagcaagt ctgtaataag ttgctctaga
      181 gattcctagc tcttcctgaa tctgcaacct cgttttacct tgtgaaataa gcatctgtac
      241 agcctctgaa gaagctttta caggtcttcc catatgtacc ccttcagctt tagcacgttc
      301 aataccagcc tttctacgct ctgctatcaa gcttctttca agctgtgcca acgttgacat
      361 aatgcctaac attgcttcac ccatagcgct tgataagtca aatccttctc taaggctgat
      421 aactgtcaca cctttagcct gtagcgcttt aaccgttgat aacacatcaa ttgtgtcacg
      481 tccaagacgg tctacagcta ccacaactac ggtgtcacct tctcgtgcat aagctaacaa
      541 actgctgaaa ccgtttctta aagaagcttt gacagaacca ctgacagctt catcagcgaa
      601 ccatttagca atgttgtacc ctgcctcttc aatttgcatt tgctggttag tgatggattg
      661 gtcatctgta ctcaccctgc aatatccaag tatcgccata atgttcccct tagaagtctc
      721 aaaagttagt ctcataagtt tagctgtctc ataatccaaa aacaaccttt tgagactact
      781 tgagggtgca ttctaccgtt acgttgaaag tgtctcaaaa ggacttcctt atgagactgg
      841 ttaatagagc gctatgaaga ttcaggaggg taactataac catcactttc taactttcac
      901 gtaggaaatc gttaagtttc ttgatagtta caccatgtga ccaactatct atatatcgtt
      961 atatagtcct gtatatagca ctatatgtag tgtctggaga gggacgacta acacgataca
     1021 gtttaccctg aaaagagact tcacagattc tttatatatt tcaataagtt atgctaaaaa
     1081 gagactatac cgatactgca tagaatttgt gtaggattgc atagatactt ggaaaaacac
     1141 cgttaaattg catagtcatt cacctgatat tgtcctgttt ttacaggtag ttgattcgga
     1201 tgttctagga atcatcctca tagttgattc ggttagttgg gcttgtacca acccctaatc
     1261 aacctcatag tgtatttaga ccttgatgat tttagacagc cgccctgttc atgcagcacg
     1321 tccacacagt gaagctattc gcgacgctca acgaaaaaaa cctaaagttc cggtacatgc
     1381 tgtactgacg gcgacaaatc cgctgattcg ttttatcggt agtgatgata tgacgcaaaa
     1441 ccgggaatta tttcaggtct ggttacagaa attagcgcag tggcatcaga ccactacgcc
     1501 ttatcttttt ttacatacgc cagacattgc ccaggccccg gaactggtac ataccctgtg
     1561 ggaagactta cgtaaaacgc ttccagagat cggagcagtt ccggctattc cacagcaatc
     1621 ttctcttttc tgaatttgcc acctatcata gacaggtgcc atcggccatt ttaaagggag
     1681 tttgtatggt aagcgcgcta tatgccgttt taagtgcgtt gttattaatg aagttctctt
     1741 ttgatgtcgt tcgcctgcga atgcagtacc gcgttgccta tggtgacggt ggttttagcg
     1801 aactgcaaag cgctattcgc attcatggta acgcggtgga atatattccc atcgcgattg
     1861 tgctgatgct gtttatggaa atgaatggcg cagaaacctg gatggtgcat atttgcggca
     1921 tcgttttgct tgctggtcgt ctgatgcatt attacggttt tcatcaccgt ctgtttcgct
     1981 ggcgacgttc cggcatgagc gccacctggt gtgcgctgtt gctgatggtg ctggcgaatc
     2041 tttggtatat gccctgggag ttggttttct ccctgcgtta gcgcacaata cgccactttc
     2101 tttttcccgg atttttacgt tatgtctcac cgcgacacgc tattttctgc ccctatcgcc
     2161 agactgggcg actggacctt tgatgaacgg gtagctgaag tcttcccgga tatgatccag
     2221 cgttccgttc ccggctattc caatattatt tccatgattg gtatgttagc cgagcgcttc
     2281 gttcaacctg gtacgcaggt ttacgatctg ggttgttctc tgggcgcggc gacgctctcg
     2341 gtgcgtcgca acattcatca tgataattgc aaaattattg ccatcgacaa ctccccggcg
     2401 atgattgaac gctgcagtcg tcatattgac gcctataaag cccctacgcc agtagacgtc
     2461 attgaaggtg atattcgcga tatcgccatt gaaaacgcat cgatggtggt gctgaatttt
     2521 accctgcaat tcctggaacc ttccgagcgc caggcattac tggataaaat ttatcaagga
     2581 ctgaacccgg gcggcgcgct ggtgctttcg gaaaaattca gtttcgaaga tgccaaagtt
     2641 ggtgaactgt tgttcaacat gcaccacgac tttaaacgtg ccaacggtta cagcgaactg
     2701 gagatcagcc agaagcgcag catgctggaa aacgtgatgc tgaccgattc cgtggaaacc
     2761 cataaagcac gcctgcataa agccggtttt gagcatagcg agctgtggtt ccagtgcttt
     2821 aactttggtt cactggtggc attaaaagca gaggacgctg catgatcgac tttggtaact
     2881 tttattctct gattgccaaa aatcatcttt cacactggct cgaaacgctg cccgcgcaga
     2941 ttgctaactg gcagcgcgag cagcagcacg ggctgtttaa gcagtggtcc aacgcggtgg
     3001 aatttctgcc tgaaattaaa ccgtatcgtc tggatttatt gcatagcgta accgccgaaa
     3061 gcgaagagcc actgagcacc gggcaaatta aacgcattga aacgcttatg cggaacctga
     3121 tgccatggcg caaagggccg ttctcactgt atggcgtcaa catcgatacc gaatggcgtt
     3181 ccgactggaa atgggatcgt gttctgcccc atctttctga tttaaccgga cgcaccattc
     3241 tggatgtcgg ctgcggcagc ggttatcaca tgtggcgcat gattggcgca ggcgcgcacc
     3301 tcgcggtggg gatcgaccct acgcaactgt ttctgtgcca gtttgaagcg gtgcgtaaac
     3361 tactgggtaa cgatcagcgc gcacatttgt taccgttagg tattgaacag cttccggcac
     3421 tgaaagcctt tgataccgtc ttttcgatgg gcgtgcttta ccatcgccgt tcgccgctgg
     3481 agcatctctg gcagttgaaa gatcaactgg tgaatgaggg tgaactggtg ctggaaacgc
     3541 tggttattga tggcgatgag aacacggtgc tggtaccagg cgatcgttac gcgcaaatgc
     3601 gtaatgtcta tttcatccct tccgcgctgg cgctgaaaaa ctggctgaag aagtgtggtt
     3661 ttgtagatat ccgcattgtg gatgtgtgcg ttaccactac tgaagagcag cgacgcactg
     3721 aatggatggt taccgaatct ctgtccgatt tcctcgaccc acatgatccg agtaagacag
     3781 ttgaaggtta tcctgcgcct aaacgcgcgg tgctgattgc gcgcaagccg taaaggtctg
     3841 gtattactgc cggatgcggc gtgaatgcct tatccggcct acaaaccctt gctaattcaa
     3901 tctattgcag ggactatgta ggcctgataa gcatagcgca tcaggcagtt ttacgtttgc
     3961 ataacctcag cgcccgtttc cgggcgctat tcacttctta cgcctgaaca gcaggcttat
     4021 caaatgccgt taacttcggt gcgttacccg tatatttttc gatatacacc agcgcagagt
     4081 taccggcaca ggcatttgcc agctgcgagc tggggatatc cgccgttaac acgttcgcac
     4141 tgccgttttt acacaagccg ttttccagat ctggccatgc accttcatgc acgcatacca
     4201 cgcctttttt gatcccgtca gtcaccaccg cgcctgtcaa aatctgacca cgtttgttcc
     4261 agacacgcac cagatcgcca ttcgcaatac caaatcgagc agcatcttcg gtgtgaatag
     4321 tgatcggttc acgatcggca accgcatatt ttttacgcag ttccgcatag ttaagctgac
     4381 tgtgtaaacg gtgtgccgga tgtgcggtca gaagctgcaa ctgcttctcg tcggcggtac
     4441 ccttccactc atcaggcgca agccaggttg ggtgtgccgg gcaatcctta tagccaaatt
     4501 tttccagcgt tttggagtaa atctcaattt tgccacttgg cgtacccagc gcatttttca
     4561 ccggatcggc gcggaaatca ccataacgaa cgtactgttc gttcttctcg ctgcggcgca
     4621 tttcgatcag tttattttgc tgccagaagg cattaaacat tggcatagtg acgcgttgcg
     4681 cacgggcccc tttctgagcg gcatcataga aaaatttcag ccacgccatt tcatctttac
     4741 cttcggtata gatctctttt ccgccaggtt tgagtaattc cgcaagatcg gcaaacacat
     4801 caaaatcgtt tcgcgcttca aattgcggag caacagcctg cttcatcggc acaatatgct
     4861 ggttgctgta atcaccggtc atcgtcaagt cattgcgctc aaacgatgtg gtgatcggta
     4921 atacgatatc tgcgtgttta gcagctgcgg tccagtagca ttcagaaacg acgatcatct
     4981 ccggtttctg ccatgcttta atcagacgat tagtatcctg gtgatgggta aagttaccgc
     5041 cacccgccca ccagatcatt ttgatattcg gatacgtttg ctctttaccg ttatgctgat
     5101 atttgccgcc aggattttcc agcgcatcga cgatacgggc aacaggaatc gccctcattc
     5161 cgccatcatc agcggcctcg ctggcctgtc cggcaatcgc cgcagacatt tccggcagca
     5221 cgccgccaac acgtgtcggg ttgccaccat tggagtaatg ataagagaaa ccaaaaccgc
     5281 cgcccggtgt accaatctgc cctaacatcg ccgccagcgt caccagcatc cagtgtttct
     5341 gttcaccata ttgctggcgc tgaattcccc agcctgccat cagcattgtg cgattcgccg
     5401 ccatcagttc agcgagtttg actatctgcg cttccggtac gccagtaatt tccgccgccc
     5461 aggccgcgct cttcggcgta ttatcgctct tacctctcag atactcttca aactgcggat
     5521 acccggtagt gtatttttca aggaacactt tatcgtgttt gccttgtgtc atcagggtat
     5581 gcgcaatccc taacatcagt gccacgtcgg tgcccatatt cggcgcgatc caggtggcat
     5641 tatcgccaaa gaagtcgatg gtttcggagc ggataggatc aatggcaatc actggtttgc
     5701 cagatttttt cagctgatgg aagtattcca gcccttgctc atcggtactg ctccaggcaa
     5761 tttttaaggt attcagcggg ttcatccccc acagcaccac aacctggctg ttttccagaa
     5821 tcagcggcca ggaggtctgc tgttcataca cctctacaga accgaccaca tgcggcatga
     5881 tcacctgtgc cgccccggtt gaataatcgc cgctatgccc ggaataaccg cccgccaggt
     5941 tcatataacg ttgcagtaag gtttgcgctt tatgcaacac gccagaagag cgccagccgt
     6001 atgatccggc aaaaatagcc gatggtccgt tagctttgcg aatgcgatcg tgttgctcat
     6061 gaatcagttt taatgcttgt tcccagctca cctgtacata ggtatcttcg ccacgacctt
     6121 tcgtcggttg cagtggatta tcgagatagc tttttctcac catcggatgc tgaatacgcg
     6181 tcgtggtgtg tacctgatcc gccgccgtag actgtaagga attcggtatg gttttcgcca
     6241 gcgcgcctgt tgaagaaaca atcttgccgt ccttcacttc tacgttcatc gcaccccaac
     6301 gtcccgcggt gaggatttta ccgtccttct cttctgccca tgcggggagc ggtgctgccg
     6361 atgtcaccac cagcgctcca gcggcaatac cgctgtgttt aataaattca cgtcttgtta
     6421 atgtcataac ttcctccctg atcaacgagg atcactgttt ctcggtaata tctttggcgt
     6481 tgtactggaa ataccgcgtt aaaatgtcca gttcgttttc gctcatgctg gttcgtgccc
     6541 ccattccttt ggcaatggac ggccacgcat tgacggtgta atggtcggcg gcaatagggg
     6601 catgacaacc agcgcaatag gtatcgtcaa gtttttcagc gtattgccat agcggtttac
     6661 ggtccgctaa tccgggatcg gtaagcgcac cctgtaaaga cgcctgacgc cattgattgc
     6721 cgtattcgtc agcctgccat tccccgttta ccgtgagcgc cttgatacct tcttcactta
     6781 atgtggctag cgccagccgt tgacctgccg ccaggtagag cgtgttttca ctgccctgca
     6841 tttgataacc ctgcaacaga acgatcggct gtttgccact ggcatcaacg acggtgagat
     6901 cggtaccagg attcacggta gccagctcgc ctatgcgaga agttttgaaa ggataaatat
     6961 gtgcgccatt agtcactgaa gtagcggcct gactttccag ctcatgcgcc gcgttgtcat
     7021 ccatttttat ttctggcgga aaatgggcaa tgcctttatg acaatcgata caggtttcgc
     7081 cgcccttttg tgctttgtta tgcattttct gcgcagattc actttgcgag gcaatatcca
     7141 tggcatcaaa agaatggcaa ctacggcacg ttgcagagtc agttgctttt aattctttcc
     7201 atactgtttc ggccatttcc tggcgatgag tttcgaactt atcgtcactg tctattttgc
     7261 cgctaacaaa ttcatgataa atatctttag atgcttttaa tttagcaaat aaataatcca
     7321 tccctgactt tggaatatgg caatctgcac attccgcacg tatccctttc tggttcgaaa
     7381 agtggacagt tccctgatat tcctcaaaag gtttactcat cgagtggcag gaaaggcaaa
     7441 atgctgtatc cgacgtttta tgtaaggctt tttgcgccag caataacccg ccgccaccaa
     7501 ccacaaccgc tatcagcaaa aacaataacc caatgcgttt tttccctcgc atagattcct
     7561 ctaaataaat tccagaacac cgaaatcatt gtataattaa atacacaacg aattaacc
//
LOCUS       DAZRUF010000196         7612 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZRUF010000196 DAZRUF010000000
VERSION     DAZRUF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.197"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            172..702
                     /locus_tag="ACHXOL_003350"
     CDS             172..702
                     /locus_tag="ACHXOL_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR6810257.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLYLKEV
                     THWMQLPEPPQEVNRG"
     gene            838..1503
                     /locus_tag="ACHXOL_003351"
     CDS             838..1503
                     /locus_tag="ACHXOL_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810258.1"
                     /translation="MLLKMVNEARKLYGEPSVRNNKFIEKIEDELEGETYTKSVGRKN
                     GADIDVISMTIKQALRVAARESKAVRRTLVDKLESMQEAHIKSGKSASGLVEYRQART
                     LKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVGFNAIPLPAIEEHYYSAGEVAEQ
                     LGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQVETFRYNEAGVHKIEELIEGER
                     KAA"
     gene            1825..2448
                     /locus_tag="ACHXOL_003352"
     CDS             1825..2448
                     /locus_tag="ACHXOL_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFR6810259.1"
                     /translation="MASQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            2533..2751
                     /locus_tag="ACHXOL_003353"
     CDS             2533..2751
                     /locus_tag="ACHXOL_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR6810260.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            2729..3799
                     /locus_tag="ACHXOL_003354"
     CDS             2729..3799
                     /locus_tag="ACHXOL_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR6810261.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            3934..5217
                     /locus_tag="ACHXOL_003355"
     CDS             3934..5217
                     /locus_tag="ACHXOL_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR6810262.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5451..>7612)
                     /locus_tag="ACHXOL_003356"
     CDS             complement(5451..>7612)
                     /locus_tag="ACHXOL_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR6810263.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         2093 a         1863 c         1897 g         1759 t
ORIGIN      
        1 gcgctacgcg ataagcaggc gttacttgaa gcctcagaaa agcgcatagc agaactggag
       61 gcacagacag ttaccgttaa agaggttgga gatgcttgat agttttgcgc tcgttgcact
      121 ggttggcatt gtagcgttga tttgggtgac atccgggaat cggaggcata agtgaacgac
      181 aaagaactga ttaaagaaat caaagagcgt atcagcagtt tggatgtgcg agacaatgtt
      241 gaacgccgtg cttatgaaat tgctctggca tcgctgacag cagagccggt ggcgtggaag
      301 gtaaccttca cgcaaattga ccgtgaatat aacacgttca ctggtatgta ttctgacaaa
      361 gcagaagtcg aacggtgggt gcggctgcat aaagcatgta attttcgggc agatataaca
      421 ccgctttata ccgcccagcc agtgccggta actccggatg cctggataag ctgtagtgag
      481 cgaatgccag aaatgggaga gcgacaatgc tatgtgttag cagctgactt taaaaacaac
      541 tacccaccaa acatccccaa cactcaggtc ggcgtatatg gcgactggtt taatgatggc
      601 aatcccactt gggatgacgg tgatggcgaa gacctgtatc tcaaagaggt aacccactgg
      661 atgcagctac cagaaccgcc gcaggaggtt aaccgtggct aacctgcaac ttgccgtcaa
      721 aggtgaataa caatcctcgc actcgcgggg atttctttta tacgatatag gggaaaaggt
      781 gtggttaaca atattgcaaa tcactcttcg tttccggctg taacaatcga cagccagatg
      841 ctgttgaaaa tggtcaatga agctcgcaag ttatatggag agccatcagt acgtaacaac
      901 aaattcatcg agaaaattga ggatgaattg gaaggcgaga cctacacaaa aagtgtaggt
      961 cggaaaaacg gggctgacat tgatgttatc tccatgacta tcaagcaggc gcttcgtgtt
     1021 gctgctcgcg aatctaaagc agttcgccga acacttgtag acaaacttga aagtatgcag
     1081 gaagcgcaca ttaaaagcgg taaatcagcg agtggacttg ttgagtatcg tcaagcgcga
     1141 acactgaaaa tgacggttga agctgttacc aatctgttcg atttgatgcc aaatcttgcg
     1201 ccggaagcaa agcagactgc tgcggcaagc ataatcaacc cgctcgttgg ttttaatgca
     1261 atacctcttc cggcaataga agagcattac tactcagcag gggaggtcgc agagcagctc
     1321 ggagtaacgg caaacaagat tggtcgcatt gctaacgcaa acaacctcaa aactgagcag
     1381 tacgggaagt tcttcctgga taaatctgcg cattccagca agcaggttga aacatttcgg
     1441 tacaacgagg ctggagtgca caaaatagag gaactcatcg aaggagagcg aaaggctgca
     1501 tgattttgac aagatagttt tccccaaatg tggggaaaag cccgaatggc gcggcttaca
     1561 gcaagataag gcctacatga tttgacaaca ccgcattaac ggggctatat tccgcttcat
     1621 ggtgctgaac acaccttgca aagcggaaac cgcacccgtc agtcatgcgg attttttatg
     1681 tccatttttc agatatggtc gggtagcgcg tataccgaaa aacagccgaa aggttaagga
     1741 tacgggccga ctttgcacgg tgttcaagta cctgaccgcc ctgctgaaca cagggctatc
     1801 tgaacaaatg caaaggacat aaaaatggcc agtcaactca tccccgtatt caacggcact
     1861 atatccaacg aaacaactct tctcgttaat gcccgtgatt tacacgaatt tttggaagta
     1921 cgtcgtgact tctctacatg gattaaaaat cgcataacag aatacggctt tgccgttgat
     1981 gtcgattaca ttttggttca ccaaaacagg gggatcaaag gacgtggagg cgatcgccgt
     2041 agcaaagact atcacctcac cctcgataca gccaaagaaa cggcgatggt cgagcgtaac
     2101 gaaaaaggcc gccagatacg ccgatacttc atcgagtgcg aaaagaaact tcgcagcatg
     2161 caaccagcgc agcaattcac agacgaagaa atcatcctcc tttgctatat gcaggtacag
     2221 atggagaatg cgcaggacat ctgcaaacgt ctgtacccga tattgaagga actgaactca
     2281 tcatacacga gcaagctgta tgacatcgcg tttgaaactt tttacacggt gacgaaaaac
     2341 agagatgtac tgctaaggga ggcgacacga cttgaccaag caagcgccct tttcgaacgg
     2401 gcaaaaccaa tgctgaaaag ccttcgggcg agacaattcg aattttaatc atcaaaggag
     2461 cttcggctcc ttttttgttg gagaaaataa accaatactc gctcccttgc gagtaattgc
     2521 ggagactttg cgatgtactt gacacttcag gagtggaacg cacgccagcg acgcccaagg
     2581 agccttgaaa cagttcgtcg atgggtacgc gagtgcagga tattccctcc tccggttaag
     2641 gatggaagag agtatttgtt ccacgaatca gcggtaaagg ttgacttaaa tcgaccagta
     2701 acaggtagcc ttttgaagag gatcagaaat gggaagaagg cgaagtcatg agcgccggga
     2761 tttaccccct aacctttata taagaaacaa tggatattac tgctacaggg acccaaggac
     2821 gggtaaagag tttggattag gccgagacag gcgaatcgca atcactgaag ctatacaggc
     2881 caacattgag ttattttcag gacacaaaca caagcctctg acagcgagaa tcaacagtga
     2941 taattctgtt acgttacatt catggcttga tcgctacgaa aaaatcctcg ccagcagagg
     3001 aatcaagcag aagacactca taaattacat gagcaaaatt aaagcaataa ggagggggct
     3061 gcctgatgct ccacttgaag acatcaccac aaaagaaatt gcggcaatgc tcaatggata
     3121 catagacgag ggcaaggcgg catcagccaa gttaatcaga tcaacactga gcgatgcatt
     3181 ccgagaggca atagctgaag gccatataac aacaaaaccg gtcgctgcca ctcgcgcagc
     3241 aaaatcagag gtaaggagat caagacttac ggctgacgaa tacctgaaaa tttatcaaac
     3301 agcagaatca tcaccatgtt ggctcagact tgcaatggaa ctggctgttg ttaccgggca
     3361 acgagttggt gatttatgcg aaatgaagtg gtctgatatc gtagatggat atctttatgt
     3421 cgagcaaagc aaaacaggcg taaaaattgc catccctaca acattgcatg ttgatgctct
     3481 cgggatatca atgaaggaaa cacttgataa atgcaaagag attcttggcg gagaaaccat
     3541 aattgcatct actcgtcgtg aaccgctttc atccggcaca gtatcaaggt attttatgcg
     3601 cgcacgaaaa gcatcaggtc tttccttcga aggggatcca cctacctttc acgagttgcg
     3661 cagtttgtct gcaagactct atgagaagca gataagcgat aagtttgctc aacatcttct
     3721 cgggcacaag tcggacacca tggcatcaca gtatcgtgat gacagaggca gggagtggga
     3781 caaaattgaa atcaaataat gattttattt tgactgataa tgacctgttc gttgcaacaa
     3841 attgataagc gatgcttttt tataatgcca acttagtata aaaaagcagg cttcaacgga
     3901 ttcatttttc tatttcatag cccggagcaa cctgtgaaca cattttcagt ttcccgtctg
     3961 gcgctggcat tggcttttgg cgtgacgctg accgcctgta gctcaacccc gcccgatcaa
     4021 cgtccttctg atcaaaccgc gcctggtacc tcttctcgcc cgattctgtc ggcaaaagaa
     4081 gcgcagaatt tcgatgctca acactatttt gcatccctga caccaggtgc tgcagcgtgg
     4141 aatccttccc cgattaccct gcctgcgcaa cctgactttg ttgtcggccc ggcgggcact
     4201 caaggtgtaa cgcataccac gattcaggcg gcggtagatg cggcaattat caagcgtacc
     4261 aacaagcgcc agtatattgc cgtgatgcct ggtgagtatc agggaacggt atatgtccct
     4321 gccgctccgg gtggaattac tctgtacggt acaggtgaaa aaccgattga tgtgaagatt
     4381 gggctttccc ttgatggtgg catgagccct gccgactggc gtcacgacgt caacccgcgc
     4441 ggcaaatata tgccaggtaa accagcgtgg tatatgtacg atagctgcca gagcaaacgc
     4501 agcgacagta tcggtgttct ctgctctgcg gtcttctggt cacaaaacaa tggcctgcaa
     4561 ctgcaaaatc tgaccatcga aaacacgctg ggcgatagcg tagatgcagg taaccatccg
     4621 gcggtggcac tgcgtactga tggtgaccag gtacagatta acaacgttaa cattctcggt
     4681 cgtcagaaca ccttctttgt caccaacagc ggtgtgcaga accgtctgga aacgaatcgt
     4741 cagccgcgta cgctggtgac caacagctac attgaagggg atgtggatat cgtttctggt
     4801 cgcggcgcag tggtgttcga taacaccgaa ttccgcgtgg tgaactcacg tactcagcaa
     4861 gaagcgtatg tgtttgcacc ggctacgctg tccaacatgt actacggttt cctcgccgta
     4921 aacagccgtt tcaatgcttt cggtgatggt gtggcgcaac tgggccgctc gctggatgtt
     4981 gatgccaata ccaacggtca ggtggtgatc cgtgatagcg ccatcaacga aggttttaac
     5041 acggctaaac cgtgggccga tgcggtgatc tctaatcgtc cgtttgcggg taataccggc
     5101 agcgtagatg ataacgacga aatacagcgc aatctgaatg acactaacta caaccgcatg
     5161 tgggaataca ataaccgcgg cgtgggtagt aaagtggttg cagaggcgaa gaagtaagag
     5221 caattaacta tttgccggat gcggcgtaaa cgccttatcc ggcctacggt tcgatgcgat
     5281 ttgtaggtcg gataagatgc gcaagcatcg catccgacaa taagtgccgg atgctgcgta
     5341 aatgccttat ctggcctaca gattcgatgc gattcgtagg tcggataaga tgcgcaagca
     5401 tcgcatccga caataagtgc cgaatgcgac ctacattcac atggcgcttt ttacatctga
     5461 cggtttttat tgaagttaat caaactaccc gccttgataa tctcgcgctc ttcagcagtc
     5521 agactttcca tatagagcgt aatttccgtt accggcgcat cttcatggat cacataacct
     5581 ttaaacgtcg tacccggatt atccagcgcc gctttaatgc cagggatgta aatgtaatcc
     5641 cccacttcaa aggttggcac ttccgccatc tgcagaggta acatccccca gttgatgacg
     5701 ttagaacggt agcgtttagt cgcgtactcc tcggcaatat tcgccagacc gccaatcaca
     5761 cgctggcagc tcgccgcctg ttcacgcgca gaaccatcgc ctggtttcac cgcatagacc
     5821 atgctgccaa tttcagtttg cagcggatca atatgctcct gaccagcaat ctgcttaatg
     5881 cgcgcaaaca cctctgtcag ctcgctgaca ttccccgcca gacgctgatt ttccagctca
     5941 gcagtcgctt tacttctgcc aacataaccg ggatcacggc gagacagggt aaactccgcc
     6001 agaccaatcg gatttgaacg ataagaagag gtttcaccgg aaggaatcag ttcgtcggtg
     6061 gtggtcactt cgtcgaggat cttcgagcac actttcagga cgatattgtc agtcagcgca
     6121 cccaattccg gccagtcttt aatgttcggc ccgtaaatca gcggttgctg agttgcccct
     6181 ttcacaaagc cctgataaac acggttttta tacggcgtta catcgaaggc gtactccggc
     6241 acgttgtccc agcaatcaag ttcgctggca gaggttaaat agccaccgtt tgccgcagtc
     6301 gcagcgatag aacgagcgtc catcaacgcc accgctgaca tctgcccatt agctggctta
     6361 gagccttcgc ggttcgggaa gttacgcgtg gtgtggcgaa tactcaaacc gttgttgatt
     6421 ggcgtatcgc ccgcgccaaa gcatgggccg cagaacgcgg ttctgatgat tgcgcctgcg
     6481 ccaatcaaat ctgctaccac accttttttg gcgagatcca taaacaccgg ctgtgatgac
     6541 gggtaaactg ccagcgagaa ggtgtcattg ccacaggatt gaccgcgcag tgcattcgcc
     6601 gccgcgatga cgttttcgta gttaccgcca gaacagcccg cgataatccc ctgctgcact
     6661 ttcaggcgac cattttccac tttatccagc agcgagagtt tggctttacc gtgcgccacg
     6721 cgttcggact caatttcaat ctcacgcaga atgtcggtca ggttctggtt cagtgtgtcg
     6781 atttcataca cgttgctcgg gtggaacggc agcgcaatca ttggtttgat ggcgcttaaa
     6841 tcaacgctga tgcagccatc gtagtacgcc atcggttgag ggttaagctg gcagtaatcc
     6901 tggccgcgac cgtgcagcgc cagccagtta tgcacttctt catcagtttg ccagacggag
     6961 cttaaacagg tggtttccgt ggtcatcacg tcaacgctgt tacggaaatc ggtagagagc
     7021 gcgctaacgc ccggtccaac gaactccatg actttgtttt tgacgtaacc gtttttgaac
     7081 accgcgccaa tgatagccag cgccacatcc tgtggcccca catacggcgc aggttttccg
     7141 gtcagatgca ccgcaaccac gcccggatag tcgatatccc aggtgtcatt aagcagctgt
     7201 tttaccaact caccgccacc ctcaccgact gccattgtcc ctaatgcacc gtaacgggtg
     7261 tggctgtctg acccgaggat cattttgccc ccgcctgcca tcatctcacg catatattga
     7321 tggatgaccg caatatgcgg aggcacaaaa ataccgccat aacgctgagc tgccgataaa
     7381 ccaaaaacat ggtcatcacc gttaatggtg ccgccgacgg cgcagagtga gttatggcag
     7441 ttggtcagca catacggcag cgggaaacgt tccattccgg acgctttagc ggtctgtaca
     7501 ataccaacaa aggtaatatc gtgagaggct aatgaatcaa acttaatttt aagtttatcc
     7561 atatttccgg acgtattatg agaagagaga atagaccagg caatagtgcc tt
//
LOCUS       DAZRUF010000197         7590 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZRUF010000197 DAZRUF010000000
VERSION     DAZRUF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.198"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            363..1262
                     /gene="yegS"
                     /locus_tag="ACHXOL_003357"
     CDS             363..1262
                     /gene="yegS"
                     /locus_tag="ACHXOL_003357"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR6810264.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(1344..2117)
                     /locus_tag="ACHXOL_003358"
     CDS             complement(1344..2117)
                     /locus_tag="ACHXOL_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR6810265.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2223..3263)
                     /gene="gatD"
                     /locus_tag="ACHXOL_003359"
     CDS             complement(2223..3263)
                     /gene="gatD"
                     /locus_tag="ACHXOL_003359"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR6810266.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3311..4954)
                     /gene="gatB"
                     /locus_tag="ACHXOL_003360"
     CDS             complement(3311..4954)
                     /gene="gatB"
                     /locus_tag="ACHXOL_003360"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR6810267.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQGGPMFSEVM
                     RYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIGIGFVGIGLVIGLMLDSIGPA
                     AKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPIAILVNVAMLLTRMTRVVNVD
                     IWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKLGDWFARDTRNFFELEGIAIP
                     HGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGPFGEPVTVGFVMGLIIGIL
                     AGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAKQARSRLQAKFGGQEFLIGLD
                     PALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGDLATIGFFVAMAVAVHRGNLF
                     RTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGGMVASMDQGGSPITWLLIQVF
                     STQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLAE"
     gene            complement(4985..5437)
                     /gene="gatA"
                     /locus_tag="ACHXOL_003361"
     CDS             complement(4985..5437)
                     /gene="gatA"
                     /locus_tag="ACHXOL_003361"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR6810268.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5447..6704)
                     /gene="gatZ"
                     /locus_tag="ACHXOL_003362"
                     /pseudo
     CDS             complement(5447..6704)
                     /gene="gatZ"
                     /locus_tag="ACHXOL_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            complement(6733..7587)
                     /gene="gatY"
                     /locus_tag="ACHXOL_003363"
     CDS             complement(6733..7587)
                     /gene="gatY"
                     /locus_tag="ACHXOL_003363"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR6810269.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
BASE COUNT         2011 a         1956 c         1698 g         1925 t
ORIGIN      
        1 aaatataata atggcatgat tattataatt gaataggatt ataataaatg ttctgtacaa
       61 catttcctac ataagtagga attacggaca ttgaggccct tcagggtaac tccatggggg
      121 ctttaatata ttatattgaa gatgccactg tttagttgaa tattaggtat atgctctttt
      181 ttgaaattta tcggtggcag ccgttagtat tcgctgtccc cattgcaagc tcctggtggt
      241 aaccactgaa tcctccatac ttgaactgac tttttatcct ccgactttca tcctgttctg
      301 actccacctt ttgttttctg ctctacacta tctacagacc aatcataaag gcacatacga
      361 tcatggcaga atttcccgcc agcttactga ttcttaatgg caaaagtact gacaatctac
      421 ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat gacgatccat gtgcgggtca
      481 cctgggagaa aggcgatgcc gcacgatatg tagaggaggc ccggaagttg ggcgtcgcaa
      541 cggtgattgc cggtggtggc gatggcacca ttaatgaagt ttctacggcg ttgattcagt
      601 gtgaggggga tggcataccc gcgctgggaa ttttgccatt aggaaccgcc aatgattttg
      661 ccaccagtgt agggattcct gaggcactgg ataaggcgct gaaactggca attgccggta
      721 acgccattgc gatagatatg gcgcaggtca acaaacaaac ctgttttatt aatatggcga
      781 caggcggatt tgggacgcgt attaccacag aaacgccgga aaaattaaaa gccgcgctgg
      841 gtggcgtctc ttacatcatt catggcttaa tgcgcatgga tactctgcaa ccggaccgtt
      901 gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc cctggtcatt ggtattggta
      961 acgggcgtca ggccggtggc ggtcagcaat tgtgtccgaa cgcgttaatt aacgatggct
     1021 tgctgcaact gcgcattttt accggcgatg aaattcttcc ggctctcgta tcaaccttaa
     1081 aatctgacga agataacccg aatattatcg aaggcgcttc gtcgtggttt gatatacaag
     1141 ccccacacga aatcactttt aatcttgatg gcgaaccgtt gagcggacaa aactttcata
     1201 ttgaaatact tccggcggcg ttgcgttgtc gattaccacc agattgtcta ttattgcgtt
     1261 aatcaaacta ttctctcttg aaccagggag aatccgaaat gccggatgcg gtgtaaatac
     1321 cttatccggc atacagaaca atactacacc gtaattaatt ttacccgtgt cttctgcaat
     1381 gccagtttat ccacctctga tacagcatca tcggtaataa taatgtcaat tttttcgatt
     1441 ggcagtacct gattaaaacc gcgacggtta aatttcgatg aatcgagaac ggcaatcact
     1501 ttattagcgg cggtgaccat cgcaccgcta atcgaataac cttcattaaa ggtcgtaata
     1561 ccattaaccg catcaatacc atcagcaccg acaaacatta aatcggcatt aatatcctgc
     1621 aatgaacgct cggcaatgga accatgcatc gagcgcgttt tatggcggac cgtgccacca
     1681 cagacgacca gagtaatgtc tttattttcg gaaagggcaa acgctgccgg gagactgttg
     1741 gtaatcaccg tgatattttt agcggtcatt aatccttcgg cgataagcat tgtcgtactt
     1801 ccgctgtcga gaataacggt catcccttca tggatcattt tgactgccgc ctgcgctatt
     1861 ctgttttttg gcgcgctggc gagctgaaat cgctctttaa acccgacttc ctgggtctcg
     1921 gtttcactat taccggacat tattttcgcc gcaccgccat gaaagcgcgt aaccacgcct
     1981 ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt ccgaggcagc aaatactccc
     2041 gccagatcct gaacaagcac ggttccctgt tcattcacta attggatgat cttattcctt
     2101 cgctcgaatg aattcatcgt catttaccca tattcaattg tggctagtgt aaacgaaggg
     2161 ggaccattgg tgaatgatct gttttgcaca ataccgggcc agcatcacgc tggcccgcgg
     2221 tttcatggaa tgagcaacac tttgcccggc atggcattac gagcgatgtc acgcaccgcc
     2281 tgggtgaagc tttcaaagct tccacggtga gcgattaatg gctccaggct taacttacgt
     2341 tctgtcagca accggctcgc cgtttcccac tcctgccccg gccaagggct ggaatagttc
     2401 atccaactgc cgataaccgt cagctcttta cgcaatattt tgccaaacgt tgccgatgtt
     2461 aaatgcagat cctgatgcaa cgtgcccacc agcgccagtt gggcatgagg accggcaatc
     2521 tctaccgcca gttcgacagt ttgcggtacg ccagccgtct cgaggataag ctgattaaag
     2581 cgcagttcgc gtaaaacgct ctgcatttgc ggcgcgctca tttcgctact gttaaatgtt
     2641 tgcatcgcac cgaaagattt tgccaatgcc agtttttctg agctaatgtc gatcgccgtc
     2701 acactctttg ctcccagcgc gacagcgcac tgaatagcca gcaggccaat ggttccggca
     2761 ccaataataa taacgttttt attctcacaa ccttgcgcta aatgaaaagc atgcaggcca
     2821 acggtaatcg gctcaataaa agccccatcc tcaataggca tatccgtggg tagagcaaag
     2881 acattttttc gcttaacgac aatatattca gcaaatccac catcacgccg cgagccaata
     2941 aaatcatatt ttgcgcactg ggaataaaac cctttcaaac actctggata agtaaaacag
     3001 ggtaataacg gcacacaggc aaccgcatcg ccagggtgta aatcatcaac accggatccc
     3061 accgcatcaa tatagccgct aaattcatgg cctaacgtta ttggataata atgtgcacca
     3121 tttttaaata tcctgggtaa atcggaacca cataagcccg agctggcaat ttttacccgc
     3181 acctcatcct gatgtttaat ttcaggaatg acgctttctg caacgcgcac gataccatca
     3241 gtatcattca ccattgattt cataaaaact cctgattgtt aagggggata acctccccct
     3301 caggtaaaaa ttattctgcg agaacggttt tctcttgttt aataaaacca cgcgctctac
     3361 gccaggtcat gaaaataccg gtaagataaa ttgcgccgat aataatgaaa ccgggaatat
     3421 tttgcgtgga gaaaacctga atcagtaacc aggtaatggg agaaccgccc tgatccattg
     3481 aagccaccat acccccggct tttaacgccc cagcattagc cgccagttgg gtgtgcaaac
     3541 caatagtttg cgtcgcgatc cacagggtga tgctcataat gatgacaccc gagattaagg
     3601 tgcggaacag atttccacga tgcaccgcga ccgccatcgc cacgaagaag ccgatggtgg
     3661 caagatcgcc aaacggcagt acctgattac ccggaacaca aacagcaatt aaaatggtga
     3721 gcgggataaa aatcaggctt gccgatacca ccgccgtatg ccccagcagt aatgcgggat
     3781 ccaggccaat caggaactcc tgaccgccga acttcgcctg taaacgacta cgggcctgct
     3841 tagcgatggg cgttaaacca tccatgatgg gtttaatcac ccgtggcatc agcagcataa
     3901 ctgccgccgt ttttaccgcc agctgcaata cacctttgac atcgtaaccc gcgaggatgc
     3961 cgataatcag ccccatcaca aaacccacgg tgacaggctc gccaaatgga ccaaatttgc
     4021 gctgaatatc gtcggcgcta aatttaattc ggttaacgcc tgggattttc tcgatgatag
     4081 catcgaccag caccgcaatc ggccccatat acgccgacgt accgtgcgga atagcaatac
     4141 cttccagctc aaagaaattt cgggtatcgc gggcaaacca gtcgccgagc ttataaacaa
     4201 acgccgcgtg aattaccaca cctgccatcc ctatcatcca tgaaccggtt gccagatgca
     4261 gcaacgcgcc ggtgaaggtc atatgccaga tattccagat atcaacattt accacccgcg
     4321 tcatacgggt cagtaacatc gccacgttaa ccagaatcgc aatcggaatc gccaccagcg
     4381 caatttgcga cgcccaggtc attggtgaag agcccggcca gccgacatcg accacatgca
     4441 gattcaggtc gaaattttcc gccatcgctt tcgccgccgg accaatggaa tccagcatta
     4501 agccaatcac aaggccaatg ccaacaaagc caatcccgat atgcagaccc gctttaaagc
     4561 aatcgcctgc cttcatgcct aatattttag aaaaaataat gatgacaatc ggaagcatca
     4621 ccgtagggcc gaggtcgaga atataacgca tgacttctga aaacataggt cccccctgta
     4681 agatagtcag aattttattt tgtaatgctt cgataccgac accagaaata aaaggcatgc
     4741 cgtgaactaa cggaatatcg ccaaaactac gatccacttt ggcagtggtg catatcaaat
     4801 gcacaccatc catataggtt tctatttcat taacccgaca ctggattaat tcaacaggaa
     4861 tactatgatt ctgacacaac tctttaattt cttccgccgc catcgtagag gtcgcaaccg
     4921 cgcctccgca agcgacaata atcttgcgtt tcataaatag tcctttttat tgttacagag
     4981 ggatttattc gtctgaatct aaaacatact ttgtgaagta ttcctttaac tgggtttcag
     5041 gaagagtgat tagtgtctcg acgatatcgg gctgttgtaa cttgccaaat aaacagcgta
     5101 aaagtttcaa ttgctgctgc ggattctcca caatcaacgc aataaccaac gatacctcca
     5161 cgtcgttatc atcatccgct tgctgaaaat gaactttatt tgttggcctt aacagataaa
     5221 tggctgacga cttagcatga atcgcctcac aatgcggtat tgcaatggcg tgctgctcaa
     5281 gcattatccc ggtagggaat tctgcttctc tggcaattaa tgcctgtggc caggtatcat
     5341 gaaccacacc tttggcgagc atctcattac cgatatgggt taaaacttcg ctacgatcga
     5401 caaaagaaat tccgctacga acaaacaggt tagtcatata ccgtccttat tccgcacagc
     5461 cgtagcgata ggcgcgcaaa acatcataaa ttttatccat aatcagctga tgcggtattg
     5521 ctgataattc cccggactga atgcgttcaa attgtttggg aagatactga ctaatcatgc
     5581 ccagtgggat gtccacgcct tcaagattca ccatcatcgt ttcgacgcta tttttaatcc
     5641 gactatgcgg ccagtaataa cgaatacgat ccgacaggct gtaacgaata tccagtaatg
     5701 aatcgttaaa acccgtacga taatattttt tccagtattg cggttcgtcg agcatcactt
     5761 cttcaattac cgccaggcaa ccgctgcgat tttcaggggc gataagttcc tgctcaattt
     5821 gtgccagtgc aaatatcgcc tcgcgtaaag caaaggttaa tgcgggaccg actttcaata
     5881 ttgcaaagtg atcgcggact aattcccaat aagccgtccg ggtctggtaa tcggtagaat
     5941 gtgcttcata aaccattcgg gtgttttcta tccattgcgc cagcggctgc gcttcctgcg
     6001 gctgataatg gataatattg ctgtgatcaa attccacgcc cggctgcacc acgatggcaa
     6061 tcacgcgtgt taacgcctct gtcagcccac gggcaataaa ggccttttga tgcgtacgta
     6121 aagtattggc ggcatcttca acatgggtga tgtgtactga ctgaatggcg ctggcctcac
     6181 cgcccggaac cggaacttca gtgccaatga catagctcag ttgctcacgc tggcaatctg
     6241 tcgccacact ttccgcagca aagcaaagca gctcgttccg caaccgtttc tggtgctaac
     6301 ggtatgggat cccccgcgca ggacattgac gcatcaagat gaattttact gaagccagca
     6361 cgaacatatg cttttaccag ctcgacggat ttttccatcg ccgcatccgc attttcttgc
     6421 tgccagcagt ttggccccag atgatcgccg ccgagaataa tgcgttcgcg tgcaaatccg
     6481 actttatcgg caatcgtaaa aacaaattcg cgaaagtctg ccggtgtcat tccggtataa
     6541 ccgccaaatt gattgacctg gtttgacgtt gcttcaatca gcactttgcg cgtgctgttg
     6601 cgatcaaatg ccagcgccgc ttcgataacc aacggatggg cagaacagac tgaacatatg
     6661 ccgatatgtt caccagcttt atgccgggca attaacgttt tcatgttttt tccttgttaa
     6721 atggcaggtg cgttatgccc tgccctcgca gccacaatcg gcaatcactt tgctcaccac
     6781 atcgcgcatt gcggatttag ccgactgcaa ataatcccgg ggatcggtcg cttcagggtg
     6841 ctcggtcagg taatttttta acgcctgcga gaaggcattt ttcagctccg ttgcaacgtt
     6901 gattttgcat atccccagtt tgatggtttg ctgaatatcc ttagtcgata accctgacgc
     6961 gccatgcagc accagcggta agttcaccca ctggcgaatg ttctccagtc gagaaaaatc
     7021 aagcgccggt gcgctggcat acatcccatg agccgtgccg atggcgaccg ccagggaatc
     7081 aattccggtt gcctcggcaa attcacgcgc ctgagcgggg ttggtataaa aaacatcggc
     7141 ttcattgact tgcacatcat cttcctggcc gccaagttgc cccagctccg cttcgacgct
     7201 gacatcaaag cgatggcaaa aatccaccac ctctttgacc cgtgaaatat tttgcgcaaa
     7261 aggcaaatgc gaggcgtcaa tcatgactga gcgcacgcca gaacgaacct tctgagcgat
     7321 atcgtcaaat ttcgtgtgat ggtcgagatg aattgccagt ggatggtgat attgcttcgc
     7381 catcgcgctg accagcgcca acagattttc tgtaccagca taagtgaatg tgccaggcgt
     7441 tccggcgatg atgaccggcg catgcaggtt ggcagcggtt tccaccacca cttgcatcgt
     7501 ttcgagattg tgaatattga atgccggaac cgcataaccg ccgcgctgtg cgttgttcag
     7561 catctgcttt gtcgatacca cgtacatttt
//
LOCUS       DAZRUF010000198         7577 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZRUF010000198 DAZRUF010000000
VERSION     DAZRUF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.199"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            63..1352
                     /gene="hpaG"
                     /locus_tag="ACHXOL_003364"
     CDS             63..1352
                     /gene="hpaG"
                     /locus_tag="ACHXOL_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR6810270.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1349..2815
                     /gene="hpaE"
                     /locus_tag="ACHXOL_003365"
     CDS             1349..2815
                     /gene="hpaE"
                     /locus_tag="ACHXOL_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR6810271.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2817..3668
                     /gene="hpaD"
                     /locus_tag="ACHXOL_003366"
     CDS             2817..3668
                     /gene="hpaD"
                     /locus_tag="ACHXOL_003366"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR6810272.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3678..4058
                     /locus_tag="ACHXOL_003367"
     CDS             3678..4058
                     /locus_tag="ACHXOL_003367"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR6810273.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4169..4972
                     /gene="hpaH"
                     /locus_tag="ACHXOL_003368"
     CDS             4169..4972
                     /gene="hpaH"
                     /locus_tag="ACHXOL_003368"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR6810274.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4983..5771
                     /gene="hpaI"
                     /locus_tag="ACHXOL_003369"
     CDS             4983..5771
                     /gene="hpaI"
                     /locus_tag="ACHXOL_003369"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR6810275.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5946..7322
                     /gene="hpaX"
                     /locus_tag="ACHXOL_003370"
     CDS             5946..7322
                     /gene="hpaX"
                     /locus_tag="ACHXOL_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR6810276.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            7332..>7577
                     /locus_tag="ACHXOL_003371"
     CDS             7332..>7577
                     /locus_tag="ACHXOL_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR6810277.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSV"
BASE COUNT         1739 a         2124 c         2151 g         1563 t
ORIGIN      
        1 tcgggaataa aagcaatcat taacatatta atgattaaga tgatcgaatc cgaggagtgg
       61 taatgaaagg cactatcttc gccgtagcgt tgaaccatcg cagccaactt gatgcatggc
      121 aggaagcgtt ccagcaatcc ccctacaaag ccccgcctaa aactgcggtc tggtttatta
      181 aaccgcgcaa tacggtgatt ggttgcggtg aaccgattcc ctttccacag ggtgaaaagg
      241 tactgagcgg tgcgactgtt gcgctgattg tgggaaaaac ggcgacgaaa gtacgtgaag
      301 aagatgcggc agagtacatc gccggatatg cgctggctaa cgacgtcagc ctgccggaag
      361 agagctttta ccgcccggca atcaaagcaa aatgtcgtga tggattctgc cccattggcg
      421 aaaccgtggc tctcagcaat gtcgataatc tgaccatcta taccgagatc aacgggcgtc
      481 ctgccgatca ctggaatacc gccgatttac aacgtaacgc cgcgcagttg ctgagcgccc
      541 tgagcgaatt tgccacgctg aatccaggcg acgccattct gctcggcacg ccacaggcgc
      601 gcgtggaaat acagccaggc gatcgcgttc gtgttctcgc agaaggtttc ccgccgctgg
      661 aaaatccggt agtggacgaa cgtgaagtga ccacgcacaa gagcttccca acgcagccac
      721 acccgcacgg cacgctgttt gccctcggcc tgaactacgc cgaccacgcc agcgaactgg
      781 aatttaagcc accggaagaa ccgctggtgt tcctgaaagc gccaaatacc ctcactggcg
      841 ataaccagac ctccgtgcgt ccaaacaata ttgaatacat gcactatgaa gcggagctgg
      901 tggtagttat tggcaaacag gcgcgtaacg tcagcgaagc cgatgccatg gattatgtcg
      961 cgggctacac cgtgtgtaac gactacgcca ttcgtgacta tctggaaaac tactaccgcc
     1021 ctaacctgcg ggtaaaaagc cgcgacggac tgacgccgat gctttcaacc atcgtgccga
     1081 aagaggcgat cccggacccg cataatctga cccttcgcac cttcgtcaac ggcgagttac
     1141 gccagcaagg caccaccgcc gatctgatct tcagcgtgcc cttcctgatc gcctacttaa
     1201 gtgaatttat gaccctgaat ccgggcgaca tgatcgccac cggcacaccc aaaggcttat
     1261 ctgacgtggt gcctggcgat gaagtagtgg tggaagtaga aggcgtgggc cgcctggtga
     1321 accgaattgt gagtgaggat acagcgaaat gaaaaaagta aatcattgga tcaacggcaa
     1381 aaatgttgca ggtaacgact acttccagac caccaatccg gcaacgggtg aagtgctggc
     1441 ggatgtggcc tctggcggtg aagcggagat caatcaggcg gtagcggcag cgaaagaggc
     1501 gttcccgaaa tgggccaatc tgccgatgaa agagcgtgcg cgcctgatgc gccgtctggg
     1561 cgatctgatc gaccagaacg tgccagagat cgccgcgatg gaaaccgcgg acaccggcct
     1621 gccgatccat cagaccaaaa atgtgttgat cccacgcgct tcccacaact ttgaattttt
     1681 cgcggaagtc tgccagcaga tgaacggcaa gacctatccg gttgacgaca agatgctcaa
     1741 ctacacgctg gtgcagccgg tgggcgtttg tgcgctggta tcgccgtgga acgtaccgtt
     1801 tatgaccgcc acatggaagg tcgcgccgtg tctggcgctg ggcaataccg cggtactgaa
     1861 aatgtcggaa ctctccccgc tgaccgctga cagcctgggt gagctggcgc tggaagccga
     1921 tattccggca ggcgtgctga acgtggtaca gggctacggc gcaaccgcag gggatgcgct
     1981 ggttcgtcat catgacgtac gtgccgtgtc gttcaccggc ggtacggcca ccgggcgcaa
     2041 catcatgaaa aacgccgggc tgaaaaaata ctccatggaa ctgggcggta aatcgccggt
     2101 gctgattttt gaagatgccg atattgaacg cgcgctggac gccgccctgt tcaccatctt
     2161 ctcgatcaac ggcgaacgct gcaccgccgg ttcgcgcatc tttattcagc aaagcatcta
     2221 cccggaattc gttaaacgct ttgccgaacg cgccaaccgt ctgcgcgtgg gcgatccgaa
     2281 cgatccgaat acccaggttg gcgcgcttat tagccagcag cactgggaaa aagtctccgg
     2341 ctatatccgt ctcggcattg aagaaggcgc aaccctgctg gcgggcggcc cggataaacc
     2401 gtccgacctg cctgcgcacc tgaaaggcgg caacttcctg cgcccaaccg tgctggcaga
     2461 cgttgataac cgtatgcgag tcgcccagga agagattttc gggccggtcg cctgcctgct
     2521 gccgtttaaa gacgaagccg aaggcttacg tctggcaaac gacgtggagt acggcctcgc
     2581 gtcgtacatc tggacacagg atgtcagcaa agtgttacgc ctggcgcgtg gcattgaagc
     2641 tggcatggtg ttcgtcaaca cccagaacgt gcgtgacctg cgccagccat ttggcggcgt
     2701 aaaagcctcc ggcaccgggc gtgaaggcgg tgagtacagc ttcgaagtgt tcgcggaaat
     2761 gaagaacgtc tgcatttcca tgggcgacca tccaattccg aaatggggag tctgatatgg
     2821 gtaagttagc gttagccgcc aagatcacgc acgtaccgtc gatgtatcac tctgaactgc
     2881 cagggaaaaa ccacggttgc cgccagggcg cgatcgacgg gcataaagag atcagcaagc
     2941 gttgccggga aatgggcgtc gataccatta tcgttttcga tacccactgg ctggtcaaca
     3001 gtgcttatca catcaactgt gcagaccatt ttgaaggcgt ctacaccagc aacgagctgc
     3061 cgcattttat ccgcgatatg acctacaact acgagggcaa cccggagttg gggcagctta
     3121 ttgccgatga agccttaaag ctcggcgtgc gggcaaaagc gcacaacatt cccagcctga
     3181 aactggaata cggcacgctg gtgccgatgc gctacatgaa tgaagacaag cacttcaaag
     3241 tggtctccat ttcggctttc tgcacggttc acgattttgc cgacagccgc aagctgggcg
     3301 aagcgattct gaaagcgatc gaacagtacg acggcaccgt ggcggtcctt gccagcggtt
     3361 cgttatcgca ccgctttatt gacgatcagc gtgcggaaga agggatgaac agctacaccc
     3421 gcgagttcga ccgccagatg gacgagcgcg tggtgaagtt gtggcgcgaa ggccagttca
     3481 aagagttctg caatatgctg ccggagtacg ccgactactg ctacggcgaa ggcaatatgc
     3541 acgacacggt gatgctgctg gggatgctcg gctgggataa atacgacggc aaggtggagt
     3601 ttatcaccga gctattccca agctctggca ccggtcaggt taacgctgtt ttcccgcttc
     3661 ccgcgtaagg acgttttatg ccgcacttta tcgttaaatg cagtgacaac atccgcgaag
     3721 aagccgacct gccggggttg ttcgccaaag tgaatccgac gctggcagcc acgggtattt
     3781 ttccgctggc gggtattcgc agccgcgtgc attgggtcga tacctggcag atggccgacg
     3841 ggcagcgtga ttatgccttc gtgcatatga cgttgaaaat cggcgcaggt cgcagcctgg
     3901 aaagccgcca gcaggcgggt gaaatgctgt ttgaactgat taaaacgcac ttcgccgccc
     3961 tgatggagag ccgcctgctg gcgttgtcgt ttgagattga agagctgcat ccgacgctga
     4021 attttaaaca aaacaacgtg cacgcattgt ttaagtagcg cgcagattgc ccggtggcgc
     4081 tgcgcttacc gggcctacaa aaccccaaac cgtacaccgt aggccggata aggcgcagcc
     4141 gcatccggca atgccacagg atatcgctat gttcgataaa cacacccaca ccctgatcgc
     4201 ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct cgctggatta
     4261 cccggagatc accatcgaag acgcttacgc ggtgcagcgt gaatgggttc gcctgaaaat
     4321 cgccgaaggt cgcacgctga aaggccacaa aatcggcctg acctcgaaag cgatgcaggc
     4381 aagctcgcag atcagcgaac cggattacgg cgcgctgctg gatgacatgt tcttccacga
     4441 tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg agctggcttt
     4501 cgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgtct acaacgccac
     4561 ggactacgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca tcgatccgga
     4621 aacccagcgc ccgcgcaaag tgttcgacac catttctgat aacgccgcca acgccggggt
     4681 gatcctcggc ggtcgtccga tcaaaccaga cgaactggat ctgcgttgga tctccgccct
     4741 gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggcgtgc tgaatcatcc
     4801 ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac aactggaagc
     4861 cgggcaaatc attctcggtg gttcgttcac ccgcccggtt ccggcgcgta agggcgacac
     4921 cttccacgtc gattacggca acatgggttc cattagctgc cgctttgttt aaggagagaa
     4981 cgatggaaaa cagctttaaa gcggcgctga aagcaggccg tccgcagatc ggattatggc
     5041 tggggctgag tagcagctac agcgcagagt tactggccgg agcaggattc gactggttgt
     5101 tgatcgacgg tgagcacacg ccgaataacg tgcaaaccgt gctcacccag ctacaggcga
     5161 ttgcgcccta ccccagccag ccggtagtac gtccgtcgtg gaacgatccg gtgcaaatca
     5221 aacaactgct ggacgtcggc acacaaacct tgctggtgcc gatggtacaa aacgctgacg
     5281 aagcccgtga agcggtacgc gccacccgtt atccccccgc cggtattcgc ggtgtgggca
     5341 gtgcgctggc ccgcgcctcg cgctggaatc gtattcctga ttacctgcaa aaagccaacg
     5401 atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac ttaccgcaga
     5461 ttctggacgt ggaaggcgtc gacggcgtgt ttatcggccc ggcggatctg agcgccgata
     5521 tgggttatgc cggtaatccg cagcacccgg aagtacaggc cgccattgag caggcgatcg
     5581 tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag caactggcaa
     5641 aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc accctgctcg
     5701 cccgcgccgc cgaagcgctg gcagcacggt ttggtgcgca ggccaccgcc gtgaagcccg
     5761 gcgtgtatta atgccggatg cgctgcgctt atccggccta cactcgcacc aaaccgtagg
     5821 ccggataagg cgtttacgcc gcatccggca aaaagccgta ccaaatcgcg gaccggataa
     5881 ggcgttacgc cgcatccgcc ataaaccttg tcgtacccta caaaaatccc attagaggaa
     5941 gaaaaatgag cgacacctca cctgccatac cggagagtat cgatccggcg aatcagcata
     6001 aagcgctgac tgccggacaa caggcggtta ttaagaagct atttcgccgc ctgagcgtct
     6061 ttctgttcgt gctgtttatc ttctcgttcc ttgatcgcat caacatcggc tttgccggac
     6121 tcacgatggg acgcgacctc ggtctgagcg ccaccatgtt tggcctcgct accaccctgt
     6181 tctatgccgc ttatgtcatc ttcggcattc ccagcaacat tatgctgagt attgtcggtg
     6241 cgcggcgctg gatcgccacc atcatggtgc tctggggcat cgcctctact gccaccatgt
     6301 ttgccactgg ccccaccagc ttgtacgtac tgcgtatact ggttggcatt accgaagccg
     6361 gctttctgcc tggtattctg ctgtatttaa ccttctggtt tccggcctat ttccgcgccc
     6421 gtgccaacgc cttgtttatg gtagcaatgc cggtaacgac agcgttgggc tcgatcgttt
     6481 ccggctacat tttgtcgctg gatggcgtca tggcattaaa aggctggcag tggctgtttt
     6541 tgctggaagg cttcccgtcg gtattactcg gcgtcatggt gtggttctgg cttgatgact
     6601 caccggacaa agctaagtgg ctgacgaaag aagacaaaaa atgcctgcaa gagatgatgg
     6661 ataacgatcg tctgacgctg gttcagccag agggagccat cagccaccac gccatgcaac
     6721 aacgcagtat gtggcgggag atcttcactc cggtggtgat gatgtatacc ctggcgtatt
     6781 tctgcctgac caacacactt agtgcgatca gcatctggac accgcagatc ctgcaaagtt
     6841 ttaatcaggg cagcagtaat atcaccatcg gcatgctggc cgccgtaccg cagatttgta
     6901 ccattctcgg gatggtctac tggagccgtc actcagatcg ccgccaggaa cgaaggcatc
     6961 acaccgccct tccttatttg ttcgctgccg caggttggtt actggcttcg gcaactgatc
     7021 acaacatgat ccagatgctg gggatcatta tggcttcgac cggatcattc agcgcaatgg
     7081 cgattttctg gacaacaccg gatcagtcca tcagcctgcg ggcacgagcg atcggtattg
     7141 cggtgatcaa cgccactggc aacattggtt cagcgttaag tccgtttatg atcggctggt
     7201 tgaaagattt gaccggcagc tttaacagtg gattgtggtt tgttgccgcg ctgctggtga
     7261 ttggtgcggg gattatctgg gcgattccaa tgcagtcctc ccgtccgcga gcgaccctgt
     7321 aaggaacgac gatgtgtgac cgtcagattg ccaatattga tatcagcaaa gagtacgatg
     7381 aaagcctggg cacggacgat gtgcattatc agtcgttcgc ccgcatggcg gcattttttg
     7441 gccgccatat gctgccacat cgccacgaac agtactttca gatgcatttc ctcaatagcg
     7501 gacagattga gctacagctt gacgatcatc gctactcggt ggaagcgccc ctgtttgtcc
     7561 tgacgccgcc gtcagta
//
LOCUS       DAZRUF010000199         7566 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZRUF010000199 DAZRUF010000000
VERSION     DAZRUF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.200"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            175..405
                     /gene="chaB"
                     /locus_tag="ACHXOL_003372"
     CDS             175..405
                     /gene="chaB"
                     /locus_tag="ACHXOL_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR6810278.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            563..1258
                     /gene="chaC"
                     /locus_tag="ACHXOL_003373"
     CDS             563..1258
                     /gene="chaC"
                     /locus_tag="ACHXOL_003373"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR6810279.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(1302..1655)
                     /gene="ychN"
                     /locus_tag="ACHXOL_003374"
     CDS             complement(1302..1655)
                     /gene="ychN"
                     /locus_tag="ACHXOL_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR6810280.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1840..3234
                     /gene="ychO"
                     /locus_tag="ACHXOL_003375"
                     /gene_synonym="iatA"
     CDS             1840..3234
                     /gene="ychO"
                     /locus_tag="ACHXOL_003375"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR6810281.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3235..3885)
                     /gene="narL"
                     /locus_tag="ACHXOL_003376"
     CDS             complement(3235..3885)
                     /gene="narL"
                     /locus_tag="ACHXOL_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR6810282.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3878..5674)
                     /gene="narX"
                     /locus_tag="ACHXOL_003377"
     CDS             complement(3878..5674)
                     /gene="narX"
                     /locus_tag="ACHXOL_003377"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR6810283.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            6013..7404
                     /gene="narK"
                     /locus_tag="ACHXOL_003378"
     CDS             6013..7404
                     /gene="narK"
                     /locus_tag="ACHXOL_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR6810284.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         1770 a         1848 c         1982 g         1966 t
ORIGIN      
        1 tttttacccc gctgtcgatt ttttactcat tggggcataa aaataagtat tacgtttaga
       61 caatgtttgt tttagcgttt cttaagaaca gtctgacatg aaaattctta tgttttggca
      121 agagtagata ttgttgacca cacttaatgt tcaactttgt aaaaggagtc aacgatgccg
      181 tataaaacga aaagcgatct gccggaaagc gtaaagcacg ttctaccgtc tcatgcccag
      241 gatatctata aagaagcgtt caacagcgca tgggatcaat ataaagataa agaagatcgg
      301 cgtgatgacg ccagtcgcga agaaaccgcg cataaagtgg cctgggctgc tgtgaagcat
      361 gaatatgcca aaggggatga tgataaatgg cataaaaaat cgtaaaaccg gtagcttagt
      421 taaagctatt cgtgcggtgt tgccttgcaa gtggtccgtg gattgcatat tgtcccgtta
      481 gtggtttcaa aatgagcggt aaaaatgtcc ggaagacacc aaaaagttgt cgcagggaag
      541 tatgcagtgg cggaggtgta aggtgataac gcgtgatttc ttgatgaatg ccgattgtaa
      601 aacggcattt ggtgccattg aagaatcact cttatggtca gcagaacaac gggcggcttc
      661 gctggcggcg acgctggctt gtcgacctaa tgagggaccg gtgtggatct tcggttatgg
      721 atcgttgatg tggaatccgg cactggagtt taccgaatta tgcaccggta cactggttgg
      781 atggcatcgc gcgttttgcc tgcgcctgac cgccgggcgc gggactgcgc accagccggg
      841 acggatgctt gcactgaaag agggcggacg caccacaggc gtcgcctatc gactaccaga
      901 agagacgctg gagcaggaac taaccctgtt gtggaagcgc gagatgatta ccggctgtta
      961 tctgccaacc tggtgtcagc ttgatcttga tgatggacgc acagtaaacg ccattgtgtt
     1021 tattatggac ccgcgacatc cagaatatga atctgacact cgcgctcagg tcatcgcgcc
     1081 gttgattgcg gcggcgagcg gtccgctggg aaccaacgca caatacctgt tttcactgga
     1141 acaggagctt atcaaactgg gaatgcagga cgatgggctg aatgatttgc tggtatcggt
     1201 aaaaaaactg ctggcggaga attatccgga tggtgtgtta cggccgggat tcgcctgagt
     1261 aaacttccct catagtgggg cgtcagacgc cccctcaaac attaaaatgt gagcacttta
     1321 tcggctgaca gcgtccattg cgccagttcc acaagagtac cgatttccac cccatcaata
     1381 agaggaagtg tgctaatccc gcgcccgtcg gtacaggttt tgcacaattt caccggtaca
     1441 ttctgagcgg taaggatctc cagcatttgc tgaatgttgt agccttcccc tggtttttgc
     1501 ccgcgcaacc cggctgtgac cgcatcagac atcaggaaca gacgcagatc cagattgctc
     1561 tcctgctctc gtaacgcaat ggccagccgc aagctgttaa acaaggattc gctcccgtaa
     1621 ggtgcgccat tggcaacgat cacgattttt tgcattattt actcctgtat tcagggaatt
     1681 agacactcat cttctatctt actgcttctg cagcgtctga ccaatcggtc acctttttaa
     1741 ggattttcct gaaagcgcga gaaaatacga caaaagttgc cagtaatcgt tattctttaa
     1801 ggctatggtt tttcattatt accggaagtt accgacgttt tgagccgttt cgttcctcgc
     1861 attatcccgt tttatttact cttgctggcg gcaggcggta cagctaacgc acaatctacc
     1921 ttcgagcaaa aagcggcaaa tccctttgat aataacaatg atggtctgcc ggatttaggc
     1981 atggctcccg aaaaccatga tggggaaaaa cactttgctg aaattgtgaa agatttcggc
     2041 gaaaccagta tgaatgataa cgggctggat actggcgagc aggcaaaagc tttcgcattg
     2101 ggaaaagtcc gcgacgcgct tagtcaacag gttaatcagc acgtagagtc ctggctatca
     2161 ccgtggggaa atgccagtgt tgacgtcaaa gtggataacg aaggacattt caccggcagt
     2221 cgtggaagct ggtttgtgcc gttacaagat aatgatcgtt atctcacctg gagccagctt
     2281 ggtcttactc agcaggatga tgggttggtg agcaatgtgg gcgttgggca acgctgggcg
     2341 cgcggcaact ggctggtggg ttataacact ttttatgaca acttgctgga cgaaaatctt
     2401 cagcgagcgg gctttggtgc cgaagcgtgg ggcgaatatt tgcgactatc ggcaaacttt
     2461 tatcagccgt ttgctgcatg gcatgaacag acagccacgc aggaacaacg gatggcgcgc
     2521 gggtatgacc tgacagcccg gatgcgcatg ccgttctatc aacacctcaa taccagtgtc
     2581 agggtagaac agtattttgg tgagcgtgtt gatttgttta actctggtac gggttatcac
     2641 aatcccgtcg cgttgagtct gggattaaat tacacccctg tgccattagt cactgtgacg
     2701 gcccagcata aacagggtga aagtggcgaa aatcaaaata acctcggact gaatcttaat
     2761 taccgctttg gtgtgccgct caaaaaacaa ctttctgcgg gcgaggttgc cgaaagtcag
     2821 tcgttacgtg gtagtcgcta tgataatccg cagcgaaata atctaccgac tcttgagtac
     2881 cgacagcgaa aaacgttaac ggtgtttctg gcgacaccgc cgtgggatct aaaacctggc
     2941 gaaacagtgc cgctgaaatt acaaatccgc agtcgttacg gtattcggca actgatttgg
     3001 cagggcgata cgcagatatt aagtttgacg ccgggcgcac aagccaacag cgcggagggc
     3061 tggacgctga tcatgcctga ctggcagaac ggggaagggg cgagcaatca ctggcgattg
     3121 tcggtggtgg tggaagataa ccaggggcag cgtgtctcct ccaatgagat cacgctaacg
     3181 cttgtcgaac cgttcgatgc attgtcaaac gacgaactgc gctgggaacc gtaatcagaa
     3241 aatgcgctcc tgatgcaccc ataccgctgc ttccacgcga gacttgagct tcattttctt
     3301 cagcatgtgc ttgacgtgca cttttactgt gctttcggtg atatccaggc ggcgggcaat
     3361 catcttgttc ggcaaaccct gggcaatcag cttgagaata tcgcgctcgc gtggggttaa
     3421 ctggttaaca tcgcgctcag tagtggcacg gttagcgcgc aagctggcgg ccagaacagg
     3481 cgttaatgct tcgcttaata ccatttcgcc agcagctgcc tgatgcaatg ctttcagcag
     3541 atcttccggt tccatatctt ttaacagata accatccgcg ccgcgtttca gtgcggtgac
     3601 cacatcttct tcatggttag agacgctgaa taccacaatg cgccctgaga gggacttttc
     3661 gcgcagttta tccagcgttt ccagaccgtt catgccgggc atattgagat ctaacaggat
     3721 cagatcggga tcaagagatt ccgccagttc aataccctgt tcgccattac tcgcttcgcc
     3781 aaccacggtg atatctggtg ccatactgat aagctgtttt acgccagttc gcagcatcgg
     3841 gtgatcgtca atcagcagga tagtagccgg ttcctgatta ctcatgggta tctccttgga
     3901 cgtctgtgaa agttttttcg ggaataaagg tgaccaccac ttcggtgcca cctgattcac
     3961 gacggcggac gcggcaatcg cctcgtaaac tttgcgcacg gtcgcgcatt attatcatgc
     4021 cgtagtgatt gctgcggatg gcattttcag gcacgccgca gccgttatcc tggacggtca
     4081 gtttgacctg attatcgttt tgcgccaccg tcaccacgac ttcactcgct tgcgaatgtt
     4141 tgagggcgtt acttaatgcc tcacgggcaa tttgcaacaa gtggattgcc tgatgcgaag
     4201 gcaccaggcg aggcggcaat tgataatcca gcttcaccgg gaagccaaat ttggcgctgt
     4261 actcttcgca actcgcctcc agcgccggac gtaatccagg ctcggtgagc tgcaagcgga
     4321 atgtggtgag caattcacgc aactgcgccc aggatgcatt cagttcgtta cggatctgac
     4381 ttaacagttc gcggctgctt tctggcagcg catcgccctg catctgtaaa caactcacct
     4441 gcatcttcat gcaagagaga gattgggcaa tagaatcatg cagttcgcgc gcaatggtgg
     4501 cacgctcttc catcacgatc aactgttgct gacgttcctg atggcgatcc agcgccagcg
     4561 tggcggtgag ttgttcaacc agggtatcca gcagttgttg ttgatcatgg ctaagatgac
     4621 gcccctgcgg cagggtcgcc agcaaaatac cgtactgcgt atgagagtca gccagccgcc
     4681 acttcagggt cgtgccgcga tcaccaacgg gtaatacgcc gcgcgggcag agctggcagc
     4741 ctttatcatc acaagtcata tctggctggc aggtaaactc ctgatgattc tcttcatcat
     4801 cagtgtcata cacccgcaat tcgatatcac gtagcagggt taaattctgt aagccgttga
     4861 gtacaggtga caggcgttca cacagcgggg cgcgggaatg caaacggcgg ttagcctgcc
     4921 ataaaaaaga gaggatctga tttttatgct ccagcccggc ggttttctcc tgaacccgct
     4981 gctcaagtac ggcataactt tcggccagtt ctgcagacat attgttcaat gcagttccaa
     5041 gcatcgccat ttcgttgcgc ccgctgatgt tcgcgcgttg ggtaaaatcg cgatgactga
     5101 cggcactcgc cattgccagc agttgccgcc acggttgtag cagtcgcgcc cgcaaccaga
     5161 taatagtgaa caccagtaaa agtgccataa ataccgccat tacccgatgg accagtacca
     5221 ctgtctcaat gcgcatttcc gtggtgcggt caaaaccaga taccagctga tcaagcccgg
     5281 caacaaactg gctgacatcc gctgacaccg tttcgcggtt ttgtgcacgc atcagtgcag
     5341 ggatcagttc attacgccag taatcttgta aaccctgtaa ttgcgccagt tgttcgtctc
     5401 gttctgctgc tcgagttaac tcggcgctaa atgccgtttg ttccatctct ttaattaagg
     5461 gcttgtcttt ctcacttaat ggcactgccg ccaacagacg gtaactttgc atgcgcagcg
     5521 atcccgcttt gttgatcgca tgggcgctgc cctgaacgcc ttgcaccagc cagccagaaa
     5581 ccgccatccc tgccagtcca atagcagtag aaagcaacac aataagcgca acctgattaa
     5641 ccagggtgag cggagagaga caacgtttaa gcatgcaaac ctcttccttc aggctttaaa
     5701 tgagcaataa ccttaatgaa tgtgacgata cattctggaa tggcagtatt ctcggctatt
     5761 ggctgaagta tacccatacc cggaaagagt tactccttat ttgccgtgtg gttagtcgct
     5821 ttacatcggt aagggtaggg attttacagc accgtgaaaa atctcataat ttttatgaaa
     5881 tcactgtact cactatgggt aatgataaat atcaatgata gataaagtta tcttatcgtt
     5941 tgatttacat caaattgcct ttagctacag acactaaggt ggcagacatc gaaacgagta
     6001 tcagaggtgt ctatgagtca ctcatccgcc cccgaaaggg ctactggagc tgtcattaca
     6061 gattggcgac cggaagatcc tgcgttctgg caacaacgcg gtcaacgtat tgccagccgt
     6121 aacctgtgga tttccgttcc ctgtctgctg ctggcgtttt gcgtatggat gttgttcagc
     6181 gctgttgcgg tgaacttacc gaaagtcggt tttaatttta cgaccgatca gctatttatg
     6241 ttgactgcgc tgccttcggt ttctggcgcg ttattacgtg ttccatactc ctttatggtt
     6301 cctatcttcg gtggtcgtcg ctggacggcg ttcagcaccg gtattctgat tattccttgc
     6361 gtctggctgg gttttgccgt gcaggatacc tccacgcctt atagcgtctt catcatcatc
     6421 tctctgctat gcggctttgc tggcgcgaac ttcgcatcca gtatggcaaa catcagcttc
     6481 ttctttccga aacagaagca gggtggcgcg ctgggtctga atggtggtct gggaaacatg
     6541 ggcgtcagcg tcatgcagtt ggttgctccg ctggtggtat cactgtcgat tttcgcagta
     6601 tttggtagcc agggcgtcaa acagccggat gggactgagc tgtatctggc gaatgcgtcc
     6661 tggatatggg tgccgttcct tgccatcttc accattgcgg cgtggtttgg catgaacgat
     6721 cttgctacct cgaaagcctc catcaaggag cagttgccgg tactcaaacg gggtcatctg
     6781 tggattatga gcctgctgta tctggcaacc ttcggctcct ttatcggctt ctccgcgggc
     6841 tttgcgatgc tgtcaaaaac gcagttcccg gatgttcaga ttctgcaata cgctttcttc
     6901 gggccgttta ttggtgcgct ggcgcgttct gcaggtggtg cattatctga ccgtctgggc
     6961 ggaactcgtg tcacgctggt gaactttatt ctgatggcga ttttcagcgg cctgctgttc
     7021 ctgaccttac cgactgacgg gcagggcgga agcttcatgg cgttcttcgc ggtcttcctg
     7081 gcgctgttcc tgacagctgg gctgggtagt ggttccactt tccagatgat ttcagtgatc
     7141 ttccgtaaac tgacaatgga tcgcgtgaaa gcagaagggg gttctgacga acgtgcgatg
     7201 cgtgaagcgg caaccgacac ggcagcggcg ctgggtttca tctctgcgat tggcgctatt
     7261 ggtggcttct ttatcccgaa agcgtttggt agctcgctgg cattaacggg ttcgccagtc
     7321 ggcgcaatga aggtattttt gattttctat atcgcctgcg tagtgattac ctgggcggta
     7381 tatggtcggc attctaaaaa ataaatctta atatttgatt atcctttgcg cggcatgatg
     7441 tcgcgctttt tttatgcgtc atttagttac aacatactca tattatatgg cttatttcgc
     7501 cggatttcat taagagccat taatatgtta cccatgggga atactcctta atacccatct
     7561 gcataa
//
LOCUS       DAZRUF010000200         7553 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZRUF010000200 DAZRUF010000000
VERSION     DAZRUF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.201"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..303
                     /locus_tag="ACHXOL_003379"
     CDS             <1..303
                     /locus_tag="ACHXOL_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR6810285.1"
                     /translation="ESYLKAQFEKLSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHL
                     VNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKILTDTSKLIYDLFGVKSEK"
     gene            666..1703
                     /gene="dusA"
                     /locus_tag="ACHXOL_003380"
     CDS             666..1703
                     /gene="dusA"
                     /locus_tag="ACHXOL_003380"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR6810286.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1837..2079
                     /gene="pspG"
                     /locus_tag="ACHXOL_003381"
     CDS             1837..2079
                     /gene="pspG"
                     /locus_tag="ACHXOL_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR6810287.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2245..3228)
                     /locus_tag="ACHXOL_003382"
     CDS             complement(2245..3228)
                     /locus_tag="ACHXOL_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR6810288.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3311..4726
                     /gene="dnaB"
                     /locus_tag="ACHXOL_003383"
     CDS             3311..4726
                     /gene="dnaB"
                     /locus_tag="ACHXOL_003383"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR6810289.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4779..5858
                     /gene="alr"
                     /locus_tag="ACHXOL_003384"
     CDS             4779..5858
                     /gene="alr"
                     /locus_tag="ACHXOL_003384"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR6810290.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6111..7304
                     /gene="tyrB"
                     /locus_tag="ACHXOL_003385"
     CDS             6111..7304
                     /gene="tyrB"
                     /locus_tag="ACHXOL_003385"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR6810291.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1814 a         1821 c         1985 g         1933 t
ORIGIN      
        1 gagagctacc tgaaagctca gtttgaaaaa ttatctcaac gtgatatgaa gaagtacctg
       61 ggagagatga agcgtctggc tgatggggga gaaatgaatt ttgatggcta tctggataag
      121 atgtcacatc tggtgaatga aggaacgctc gatcctgata ttttaagcaa aatgcgagat
      181 gctgcaccac aattaattag cttcgcgaag tcgtttgacc caacctcaaa ggaagagatt
      241 aaaatactta cagacacttc taaattaatt tatgatttgt tcggggttaa atcggagaaa
      301 taatatgtga agttcttcga tagtatggaa ggcattatat aaaaggaccc aatatttatt
      361 gggttctttt ttctctatca atgctattag cagggagata tatcaccaga gtttaatgtg
      421 tgatttttta tttatcgtcg aacctggatt gtttatcatt ggccttaaca aagttaacgg
      481 ctaataagat tatttccatc acttcgtgag agcttcatgc cttgagagga tctcaatttt
      541 cttttgcaat gagacaggcg cttcctgttg ttatggtata gtaccccgct attgagcctc
      601 ctgaatagtg atgctgaata acataacccc atgatatatc gataaaataa tctctacatt
      661 tgaaaatgca cggtaattct gaaatgcaaa aaatcaacca aaccagcgca atgcctgaaa
      721 aaactgacgt tcactggagt ggtcggttta gcgttgcacc aatgctcgac tggacggaca
      781 gacattgccg ctatttcttg cgtctgcttt cccgcaatac gttgctgtat accgaaatgg
      841 tgaccacagg ggcgattatt cacggtaaag gtgattacct ggcgtacagt gaagaagaac
      901 atccggtagc gttgcaactc ggcggtagcg atccggcggc gctggcacag tgtgcgaagc
      961 tggcagaagc gcgtggatat gatgagatca acctgaatgt cggctgcccg tctgaccggg
     1021 tgcagaacgg catgtttggt gcgtgtctga tgggtaatgc gcagctggtt gccgactgcg
     1081 tgaaagcgat gcgcgatgtg gtgtcgattc cagtgacggt gaaaacgcgt attggcatcg
     1141 atgaccagga cagctatgaa tttctctgcg atttcatcaa caccgtttcc ggcaaaggcg
     1201 agtgtgagat gtttatcatc cacgcacgta aagcctggct ttcggggtta agcccgaaag
     1261 aaaaccgtga aatcccgccg ctcgattatc cgcgtgtgta tcaactgaag cgtgactttc
     1321 cgcatctgac aatgtcgatt aacggtggta tcaagtcgct ggaagaggcc aaagcacacc
     1381 tgcaacatat ggatggcgtg atggtcgggc gcgaggcgta tcagaatccg ggtattctgg
     1441 cggcggtaga ccgggagatc tttggttcct cggataccga tgccgatccg gtggcggtag
     1501 tgcgcgccat gtatccgtac attgagcgtg aactcagcca ggggacgtat ctcggccata
     1561 ttacccggca tatgttgggc ttgttccagg gtattcctgg cgcgcggcag tggcggcgtt
     1621 atttaagtga aaatgcccat aaagcgggtg cagacattaa tgtgctggaa cacgcgctca
     1681 aactggtggc ggataagcgt taacttttca ccaaaaagta gtcaaattca ccacgccctg
     1741 cgcaccgtcg cggggcgttt tgctgttaaa tcaatagatt atttttggca tgattcttgt
     1801 aatgccagca agagatttca tatttgggag agcatcatgc tggaactact ttttgtgatt
     1861 ggcttttttg tcatgctgat ggtcaccggc gtttcgttgc tgggcattat cgccgcgctg
     1921 gttgtggcga cggccattat gttcctcggc ggtatgctgg cattgatgat taagttgctg
     1981 ccgtggttac tactggcgat tgcggtggtg tgggttatta aggcgattaa agcaccaaaa
     2041 gtgccgaaat atcagcgtta tgaccgctgg cgttactaag ggattgtgcg gatgatcaca
     2101 acctaaggtt ttatccttag aacaaaatag gaattgataa tcaaatctgt cactattgcg
     2161 cctctaacag attcatcgtg ctgtacccta catacagccg aactataaaa agaaagggct
     2221 tcccaggtgg aagccctatt tcttttatgg aatcaacagg ctggaacctt gcgtcgcccg
     2281 gctttccaga atctcatgcg cacgctgcgc atccttcagc ggatatttct gctgctcggc
     2341 gacatcgacc ttaatcacac cgctggcaat caaagagaac agttcattac tggcctcggt
     2401 taattcctcc cgcgtggtga tatagccttg cagggaaggg cgtgtcacat acaacgagcc
     2461 tttttgattg agaatgccta agttcacacc ggtaaccgca cctgatgagt tgccaaaact
     2521 gaccattaag ccgcggcgtt gcaggcaatc cagcgaccgt tcccaggtgt ctctgcccac
     2581 ggaatcgtac accacgcgca ctttcttacc gccggtgatc tcttttaacc gctcgaccag
     2641 atcctcttca cgatagttaa taacctgcca cgcgcccgct tttagcgcgc tctgcgcttt
     2701 ttgcgcggtt cctacggtgc cgataagttt cgcgcccagg gcttttgccc actggcaggc
     2761 aattaagcca acgccgccag ccgctgcgtg gaacaggaac tgttcatcgg gtttaatttc
     2821 ataggttttg cgcagcagat aataaaccgt taagcctttc aggaaggatg ccgcagcttg
     2881 ctcaaaagaa attgccgcag gcagaatcgc cgctttatcc gcattaatgt tatgcacaga
     2941 gctgtaagcg cctaacgccg actgcgcata gactacacga tcgcctgcct taatatgctt
     3001 tacaccactg ccgactttac tcacgatgcc tgccgcttcg gtacctaatc cgctgggtag
     3061 tgatggcggc gggtaaaggc cgctgcggat atacgtgtcg ataaaattga tgccgatggc
     3121 tttattttcg acctggattt cattctctgc cggatcggca ggagtgaact ctacggcttg
     3181 aagtacttcc gggccaccgt gcttgtgaaa ttcaattcgt gttgccatgt gtcctccaga
     3241 acgtatcgtc agggtctgct tcatatggta aagtttcgac ccattcttta tctcggtaac
     3301 tccattcact atggcaggaa ataaaccctt caacaaacag caggctgaac cccgcgaacg
     3361 cgatccacaa gttgccgggc tgaaagtgcc tccgcactcg atcgaagcgg agcagtcggt
     3421 gttgggcggt ttaatgctgg ataacgaacg ctgggacgat gtagccgagc gtgtggtggc
     3481 agacgatttt tacacccgcc cacaccgcca tatctttact gaaatggcgc gtttgcagga
     3541 aagcggtagt cctatcgatc tgattaccct tgcggaatcg ctggaacgcc aggggcaact
     3601 cgatagcgtc ggtggttttg cttatctggc agagctgtca aaaaatacgc caagtgcggc
     3661 gaacatcagt gcttatgctg acatcgtgcg tgaacgtgcc gttgtccgtg agatgatctc
     3721 ggttgcgaat gagattgctg aagccggttt tgatccgcag gggcgtacca gcgaagatct
     3781 gctggacctt gctgaatccc gcgtctttaa aattgccgaa agtcgtgcaa acaaagacga
     3841 agggccgaag aacatcgccg atgtgctcga cgcaaccgtg gcgcgtattg agcagttgtt
     3901 tcagcagcca cacgatggcg ttaccggagt aaacaccggt tatgacgatc tcaacaaaaa
     3961 aaccgctggc ttgcagccgt cggatttgat catcgtcgcc gcgcgtccgt cgatgggtaa
     4021 aacaacattt gcgatgaacc tcgtcgaaaa cgcggcgatg ttgcaggata aaccagtact
     4081 tatcttctcg ctggagatgc cttcagaaca gattatgatg cgttctctgg cgtcgctgtc
     4141 gcgcgttgac cagactaaaa tccgtaccgg gcagctcgat gatgaagact gggcgcgcat
     4201 ttccggcacc atgggtattt tgctcgaaaa acgcaatatc tatatcgatg actcctccgg
     4261 cctgacgcca acggaagtgc gttcccgcgc acgccgtatt gcccgtgaac acggcggcat
     4321 cgggcttatc atgatcgact acctgcaact gatgcgcgta ccggcgcttt ccgataaccg
     4381 tacgctggaa attgcagaaa tctctcgctc gctgaaagca ctggcgaaag aactgaacgt
     4441 gccggtggtg gcgctgtccc agttgaaccg ttctctggaa caacgtgccg acaaacgccc
     4501 ggtcaactcc gacctgcgtg aatctggctc tatcgagcag gatgcggact tgattatgtt
     4561 tatctatcgt gatgaggtgt atcacgaaaa cagtgattta aaaggcatcg cggaaattat
     4621 tatcggtaaa caacgtaacg gcccaatcgg gacggtacgc ctgaccttta acggtcaatg
     4681 gtcgcgcttc gacaactatg cggggccgca gtacgacgac gaataataat tattttatga
     4741 attaggtaat taaagcaaac acttatcaag gaacacaaat gcaagcggca actgttgtga
     4801 ttaaccgccg cgctctgcga cacaacctgc aacgtcttcg tgaactggcc cctgccagta
     4861 aaatggttgc ggtggtgaaa gcgaacgctt atggtcacgg tcttcttgag accgcgcgaa
     4921 cgctccccga tgctgacgcc tttggcgtag cccgtctcga agaagctctg cgactgcgtg
     4981 cggggggaat caccaaacct gtactgttac tcgaaggctt ttttgatgcc agagatctgc
     5041 cgacgatttc tgcgcaacat tttcataccg ccgtgcataa cgaagaacag ctggctgcgc
     5101 tggaagaggc tagcctggac gagccggtta ccgtctggat gaaactcgat accggtatgc
     5161 accgtctggg cgtaaggccg gaacaggctg aggcgtttta tcatcgcctg acacagtgca
     5221 aaaacgttcg tcagccggtg aatatcgtca gccattttgc gcgcgcggat gaaccaaaat
     5281 gtggcgcaac cgagaaacaa ctcgctatct ttaatacctt ttgcgaaggc aaacctggtc
     5341 aacgttccat tgccgcgtcg ggtggcattc tgctgtggcc acaatcgcat tttgactggg
     5401 tgcgcccggg catcattctt tatggcgtct cgccgctgga agatcgctcc atcggtgccg
     5461 attttggctg tcagccagtg atgtcactaa cctccagcct gattgccgtg cgtgagcata
     5521 aagccggaga gcctgttggt tatggtggaa cctgggtaag cgaacgtgat acccgtcttg
     5581 gcgtagtcgc gatgggctat ggcgatggtt atccgcgcgc cgctccatcc ggtacgccag
     5641 tgctggtgaa cggtcgcgaa gtaccgattg tcggacgcgt ggcgatggat atgatctgcg
     5701 tagacttagg tccacaggcg caggacaaag ccggggatcc ggtcatttta tggggcgaag
     5761 gtttgcccgt agaacgtatc gctgaaatga caaaagtgag cgcttacgaa cttattacgc
     5821 gcctgacttc aagggtcgcg atgaaatacg tggattaatc gttctgtaat atttgattgt
     5881 ctgtgccgga tgcggcgtga atgccttatc cggccaataa aatcctaaaa attcaataag
     5941 ttgatgttct ttcatgctct tataaaggtc gtgcctctgg cggatgttcg tttgtcatga
     6001 gtctcactct gttgctaatt gccgttcgct cctgaacatc cactcgatct tcgccttctt
     6061 ccggtttatt gtgttttaac cacctgcccg taaacctgga gaaccatcgc gtgtttcaaa
     6121 aagttgacgc ctacgctggc gacccgattc ttacgcttat ggagcgtttt aaagaagacc
     6181 ctcgcagcga caaagtgaat ttaagtatcg gtctgtacta caacgaagac ggaattattc
     6241 cacaactgaa agccgtggcg gaggcggaag cgcgcctgaa tgcgcagccc catggcgctt
     6301 cgctttattt accgatggaa gggcttaaca gctatcgcca tgccattgcg ccgctgctgt
     6361 ttggtgcgga ccatccggta ctgcaacaac agcgcgtagc aaccattcaa acccttggcg
     6421 gctcaggggc attgaaagtg ggcgcggatt tcctgaaacg ctacttcccg gaatcaggcg
     6481 tctgggtcag cgatcctacc tgggaaaacc acgtagcaat attcgccggg gctggattcg
     6541 aagtgagtac ttacccctgg tatgacgaag cgactaacag cgtgcgcttt aatgacctgt
     6601 tggcgacgct gaaaacatta cctgcccgca gtattgtgtt gctgcatcca tgttgccaca
     6661 acccaacggg tgccgatctc actaatgatc agtgggatgc ggtgattgaa attctcaaag
     6721 cccgcgagct tatcccattc ctcgatattg cctatcaagg atttagtgcc ggtatggaag
     6781 aggatgccta cgctattcgc gccattgcca gcgctggatt acccgctctg gtgagcaatt
     6841 cgttctcgaa aattttctcc ctttacggcg agcgcgtcgg cggactttct gttctgtgtg
     6901 aagatgccga agccgctggc cgcgtactgg ggcaattgaa agcaacagtt cgccgcaact
     6961 actccagccc gccgaatttt ggtgcgcagg tggtggctgc agtgctgaat gacgaggcat
     7021 tgaaagccag ctggctggcg gaagtagaag agatgcgtac tcgcattctg gcaatgcgtc
     7081 aggaattggt gaaggtatta agcacagaga tgccagaacg caatttcgat tatctgctta
     7141 atcagcgcgg catgttcagt tataccggtt taagtgccgc tcaggttgac cgactacgtg
     7201 aagaatttgg tgtctatctc atcgccagcg gtcgcatgtg tgtcgccggg ttaaatacgg
     7261 caaatgtaca acgtgtggca aaggcgtttg ctgcggtgat gtaatgcagg aaagcaggct
     7321 ggagttaccc agcctgcagt gaaattaaac tgtcgtcgct ttcactattt ctttgtagat
     7381 gatttttttg atgccgttgt tctacgtgag agataataaa acgttgtcag ttcttttatt
     7441 gttaagttta tcccaattat ctggaattcc tttccctatt ttttcgtgtg gagtaataat
     7501 ttctgcacat ctaaccatta ttaaatttaa taataagtat cgggtttgtt acg
//
LOCUS       DAZRUF010000201         7484 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZRUF010000201 DAZRUF010000000
VERSION     DAZRUF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.202"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHXOL_003386"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHXOL_003386"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR6810292.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHXOL_003387"
     CDS             complement(1672..2667)
                     /locus_tag="ACHXOL_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR6810293.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHXOL_003388"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHXOL_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHXOL_003389"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHXOL_003389"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR6810294.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHXOL_003390"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHXOL_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR6810295.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHXOL_003391"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHXOL_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR6810296.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         2076 a         1763 c         1715 g         1930 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgct
//
LOCUS       DAZRUF010000202         7384 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZRUF010000202 DAZRUF010000000
VERSION     DAZRUF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.203"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..157
                     /locus_tag="ACHXOL_003392"
     CDS             <1..157
                     /locus_tag="ACHXOL_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810297.1"
                     /translation="FSEQIFKITGDENVAKNELEPWTPEGNAPNYCWWEVDPVDAANE
                     AMSYHND"
     gene            190..408
                     /locus_tag="ACHXOL_003393"
     CDS             190..408
                     /locus_tag="ACHXOL_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR6810298.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            479..796
                     /locus_tag="ACHXOL_003394"
                     /pseudo
     CDS             479..796
                     /locus_tag="ACHXOL_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(829..1467)
                     /gene="ompW"
                     /locus_tag="ACHXOL_003395"
     CDS             complement(829..1467)
                     /gene="ompW"
                     /locus_tag="ACHXOL_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR6810299.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1707..1796
                     /locus_tag="ACHXOL_003396"
                     /pseudo
     CDS             1707..1796
                     /locus_tag="ACHXOL_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1824..2567
                     /locus_tag="ACHXOL_003397"
     CDS             1824..2567
                     /locus_tag="ACHXOL_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR6810300.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2597..3136
                     /locus_tag="ACHXOL_003398"
     CDS             2597..3136
                     /locus_tag="ACHXOL_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR6810301.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3241..3639
                     /gene="yciA"
                     /locus_tag="ACHXOL_003399"
     CDS             3241..3639
                     /gene="yciA"
                     /locus_tag="ACHXOL_003399"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR6810302.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3679..4398)
                     /gene="tonB"
                     /locus_tag="ACHXOL_003400"
     CDS             complement(3679..4398)
                     /gene="tonB"
                     /locus_tag="ACHXOL_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR6810303.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4489..6009
                     /locus_tag="ACHXOL_003401"
     CDS             4489..6009
                     /locus_tag="ACHXOL_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR6810304.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6042..6812)
                     /locus_tag="ACHXOL_003402"
     CDS             complement(6042..6812)
                     /locus_tag="ACHXOL_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR6810305.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHTIANGLRIQMTFQGVEFRSIVGQTDVSAATG
                     KTATTNDGGATSKKNTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTSSKSVSV
                     KNGESTFSDAANEQASKVLKPGNALRKYSVNPNKKGTY"
     gene            complement(6986..>7384)
                     /locus_tag="ACHXOL_003403"
     CDS             complement(6986..>7384)
                     /locus_tag="ACHXOL_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR6810306.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
BASE COUNT         1936 a         1669 c         1714 g         2065 t
ORIGIN      
        1 attctctgag cagattttta aaattactgg cgacgagaat gtggcaaaaa atgaattaga
       61 accgtggaca cctgaaggaa acgcaccaaa ttattgctgg tgggaggttg atccggttga
      121 tgctgcaaat gaagccatga gttaccacaa cgattaatgt cgggaggccg cccgaaaggg
      181 cggtaagaaa tgactacatt attcagaaaa gaatatccgc aaaaaagtag ggcgacagaa
      241 tttttgtttc tcattctgtt tatcgtattg atgataccga tatcccctct aatttttgtc
      301 tgggcaatcg ggaaaataat tgagccagtt actgaattgt ataccgacgt tgtatgggcg
      361 tcgttcaaca cactgcacaa taaaattaat ccgtataagg aaaactgata tggcaacttt
      421 gacaaaaaaa gaacgggcat ggttgaacga attacaggaa gttcttgatc gctgtcctgt
      481 ggtgtcatcc tgccgcgatt gcgttcttag tccgtggcta ttgcaccacc atcacgcgaa
      541 agggacagct aagcgcggcg ggatggttaa gccagcaaca ttaaccgttg catttaaaaa
      601 agcccgggat tctgtggatt acaactggcg tgctaatggc accccaccct ctttccatga
      661 gcagagatct ttatcagagc gattgttcag agagcagggg gttgatacca aaattttgct
      721 aggccattcg aatcaaaaaa tgaccgatat ttacaacgat gtacgcggta aggagtggaa
      781 aaaactggtc atttgataac cagttttgca gaggttttgc agagaaaatt aaaagcggta
      841 cccggcagag aacataaaca cccacggatc cagtcgtacc gagtctttta cggtagtaac
      901 accagatttg tacttagccg tggtatcgat atccatgtac cacactgaca tgttaaccaa
      961 ccagtcacgg ttaatcagat aatcaacccc cacctgcccg gcagctcccc agtaatcttt
     1021 cagactgaga tcggaaagcc ccgcctcttt gccatgatcg ttaaatccat tatcaaagaa
     1081 ggtggtgtag ttaatacctg ccccaacata aggacggaat ttgctgccgg catcaccaaa
     1141 ataccactgc gccatcagtg ttggcggcag atgatgaacg gttgcaatat cgccggtcgc
     1201 ccgggtgccg attttatggc ggaacggcgt cgctgccagt aattccacac caatgttgtc
     1261 ggtcgccata taagtaaacg tcaggcccag ttgcgtgtta ttggtcacgc tgaatccacc
     1321 cagacttcct aacgtaccac cagcaccttc tgttggacgt acggttgcag aacctgcacg
     1381 cataaaaaat tcgcctgctt catgcgcaaa ggcactgcca gagagaagag ttgttactgc
     1441 caaagccgcc actgttaact ttttcatatc cgctccgtcg ttatggttat aaaaacgtga
     1501 cgaatatacc tacattggag taacaaatga tcctacatag atcacattac atatgataat
     1561 tcacccttta ttgatctgga ttaatttaga agttgcattg agaatcctgg ttaacgtgat
     1621 ttaaatcaat ttttagatat tttcgctacg caaatattaa ctcgttcgcc acttgccgcg
     1681 gtttgcccac gcctgaaggc cagcaattac ctccgcttta caaagatatg cttttccata
     1741 caagacctga tgctgccacc cgaggcttaa ccagggtaca attgcccgct aattaacacc
     1801 tgcataaaac tcaaggagag tgcatgtcta tcacggcgca gtccgtatac cgtgacaccg
     1861 gaaatttctt ccgtaatcaa tttatgacca ttctgttggt atcgttgcta tgtgcgttta
     1921 tcacagtggt gttagggcat gttttctcac ccagtgatgc acagcttgcg cagctcaatg
     1981 acggcgtgcc cgttagcggc agtagtgggt tgttcgacct ggttcagaat atgtcaccgg
     2041 aacaacaaca aattttgctg caggcttcag cggcgtccac tttttcagga ttaatcggta
     2101 acgccattct cgccggaggc gtaatattaa ttatccagct ggtgtctgcg ggtcagagag
     2161 tcagtgcgct gcgagctatt ggtgccagtg cgccgatatt gcccaagtta tttattctga
     2221 tttttctgac taccctttta gtacagattg gcatcatgct ggtggtcgtt ccgggaatta
     2281 tcatggccat tttactggcc ctggcaccgg tgatgctggt acaggacaaa atgggcattt
     2341 ttgcctcgat gcgtagcagt atgcggctga cttgggcgaa tatgcgtctg gtggcacccg
     2401 cagtactgag ctggttgctg gcaaaaacac tgttgctgct ttttgcctct tcttttgccg
     2461 cattaacccc ggaaattggt gccgtactgg cgaacacctt gagcaacctg atttcagcca
     2521 tattgctcat ctatctgttc cgcctgtata tgttgattcg ccaataacct taagcatttg
     2581 attacggaat cgtaaaatga agcagtttct tgatttttta ccgctggttg tctttttcgc
     2641 gttttacaag atttatgaca tctatgcggc tactgcggcg ctgatcgtcg ccacggcgat
     2701 tgtgcttatc tatagctggg ttcgctttcg taaggttgag aagatggccc tgatcacttt
     2761 tgttctggtg gtcgtcttcg gtggcttgac gctgttcttc cacaatgatg agtttattaa
     2821 atggaaggtt acagtcattt atgctctgtt tgcgggagcc ctgttagtca gccaatgggt
     2881 gatgaaaaag ccgctaattc agcggatgct gggtaaagaa ctcacgctgc cgcaaccggt
     2941 atggtcgaag ctaaatctgg cctgggctgt tttctttatc ctttgcggtc tggcaaacat
     3001 ctacatcgca ttctggctgc cgcaaaatat ttgggtcaac tttaaagtct ttggcctgac
     3061 cgcccttacc ttaatcttca cattgttaag cggtatctat atctaccgcc acatgccgca
     3121 ggaagataaa tcctaaccag actgccagtc cttcggggct ggcgctgtat ctcctctgtt
     3181 ctcctcagga aaatcatagt agcatcgcgc ctgtgatttt ccttttaagt cggttttacc
     3241 atgtctacaa cacataacgt ccctcagggc gatcttgttt tacgtacttt agccatgccc
     3301 gccgatacca atgccaatgg tgacatcttt ggtggttggt taatgtcaca aatggatatt
     3361 ggcggcgcta ttctggcaaa agaaattgcc cacggtcgcg tagtgactgt gcgggttgaa
     3421 ggaatgactt tcttacggcc ggttgcggtc ggcgatgtgg tgtgctgcta tgcacgctgt
     3481 gtccagaaag ggacgacatc ggtcagcatt aatattgaag tgtgggtgaa aaaagtagcg
     3541 tctgaaccaa tcgggcaacg ctataaagcg acagaagcat tatttaagta tgtcgcggtt
     3601 gatcctgaag gaaaacctcg cgccttacct gttgagtaat agtcaaaagc ctccggtcgg
     3661 aggcttttga ctttctactt actgaatttc ggtggtgccg ttaattttaa acaggatatt
     3721 caccacaatc ccactgcctg gcttacccgg ctcataacgc catctgcgca tcgcattttt
     3781 cacctcacgc tcaaacatgt tcgcaggctt ggctgagagg atttgtacgt tatccacgcg
     3841 accatccggc gtgacgtcaa atttaacttt aacctgccct tcaatgcgca atgcctgtgc
     3901 tcgtgccgga tactgcggct gattacggct taatgcgcgt ggtcctgaag ccacactggt
     3961 aaccggcttg ctggttgcag ccgttgccgt acttgatgtg gggcgcgcgg gtgccgtatt
     4021 ttcaaacggt gatgccggac gcgactctac gggtttgaca tcgcgttttg gctgctcctg
     4081 tacctttttc accggcttcg gttttggctt aggtttcggc ttcggctttt caatgaccac
     4141 cggtgcttct tttggcggtt cggggatcgg ctcaggttcc ggttctggct ctaccaccgg
     4201 ctccggtggc ggctgaacgg cttgtggcgg ttcgagatca gcaggcgcaa ccatcgtgac
     4261 agaaattggc tgcgcaggcg caggtagttc aataacctga tgtaccgagg tatagagcag
     4321 acccgccaca acagcaccat gaatgcagac cgaaagtaac gtcggccagg ggaagcggcg
     4381 aggtaaatca agggtcattg tattgtagct attcggcatt gtagcattag ccataacgtt
     4441 tataagttca tctttaaatg ataatcaatt gcatgggagt ttatcaccgt gagaaccgta
     4501 ataacatact tgagattctc atcagccata caaggcgctg aaggtgcaga ttcaaccaga
     4561 cgacaaaacg acctgttcaa gcagtggctg aagaagaatg gcgatgctca aatagtagcg
     4621 tctttcagtg atgaagggtt gagtggttac aaaggtaaac accttactgg tcagtttggt
     4681 gacatgttag cccgtattga ggctggggag tttccagaag gtacaattct gctagttgag
     4741 agtatcgacc gtataggccg attagagcat cttgaaacag aagccttgat gaaccgcatc
     4801 ttaggcaacg ggatagagat tcacacattg caagatggcc ttatctacac aaaggatgct
     4861 ttagccgatg accttggcat aagcatcatc cagcgtgtca aagcctacat agcgcatcag
     4921 aaatctaagc agaagtcttt ccgtgtttct cagaaatggg ggcaacgtgc aaaacttgcc
     4981 cttgctggtg aacaacgtct tacaaagatg gttcctggat ggattgaccc tgagactttt
     5041 aaactcaatg aacacgctga gactgtgaga ttgatattca aactgctgct agatggtgaa
     5101 agtctgcata acattgcacg tcaccttcaa agcaacggta taaagtcgtt tagtcgccgt
     5161 aaagatgcta atgggttctc tgttcactct gtacgcacca ttctaaggtc agagacaaca
     5221 ataggcacgt taccagcatc acaacgtaat gaccgccccg ctataccgaa ctactacgaa
     5281 ggtgttgtag atataccaac gttcaataaa gctcaagaga ttctcgacaa gaatcgtaaa
     5341 ggccgtacac ctgcaagtga caacccacta acgattaaca tcttcaaagg tctgtttagg
     5401 tgtcagtgtg gggctagtgt ccatcctacc ggaacaaaga ataagtatgc tggggtctac
     5461 aggtgcaata accacttaga cggtcgctgt gatgttccac cgttgaagcg taaaccgttt
     5521 gaccgatgga tgattgataa ttttctgggg atgattgacg tggggaatga tggagaatca
     5581 gagagaaaga ttgcagcgtt acagcatgag gttgaaattg tcacagccag aatcaagaaa
     5641 gctaccgccc tacttcttga gatggatgat attgatgaac taaaaattca gcttaaggaa
     5701 ctgaaccaga agcgcacaga actacagacc acgattgata acatgaggcg taaggcttca
     5761 ctcactgata aggaactacc ccagcttaaa gatattgacc ttatgactaa agcgggtcgt
     5821 gtcgagtgtc agttgatttt gtccaagcat ctaaaagggc ttacattggg taaggattcg
     5881 gtaactgtaa cgctacagaa cgacactgaa atcaccatac ctacaaaccc gttaccgcta
     5941 aatgatgggt caccaatctt tgaaattgct gataaagagc tactggatat tgacgcttat
     6001 caactgtaat tttcaggggc tatgaagccc ctttttatgt ctcaataagt gcccttcttg
     6061 ttagggttca cagagtactt tcgcagagca ttacccggct taagcacctt tgaagcttgc
     6121 tcattagcgg catctgagaa ggtgctctca ccatttttaa cgctaacgct cttagagctc
     6181 gttacacggg cttttagcgc ttcatctgat aactcacttg ccgaatagcc aaaaagctct
     6241 ttacaggtca gcatagggct tccaaggctc gttgtagtgt ttccggtgtt ctttttactg
     6301 gtagcaccgc catcattcgt ggtagcggtc tttccagtag cagccgaaac atcagtctga
     6361 cctacgattg aacgaaactc tacaccttgg aaggtcatct gaatacgaag accgttagca
     6421 atggtatgct ctttactcgc tttaaactga gtaatgatac tatcagtgat agctattcca
     6481 tctctacaaa caatagtgat gatttgcttc tggtctcgcc attgttcaac cgtagaaata
     6541 aaggtttcta caagttgccc ctgtctggtt aatagaactg aacccgtcga atcaaccact
     6601 acaacaccgc taatagagat ggtcttaggt gctctctgaa cattatcagt gatagtttgc
     6661 cccgattgca tagcctgagt tgtcaccatc atcggtgagt catagtccat ctgttcagta
     6721 gctgttaagg caagaaaaga ttccacatta tctctaaggt gaaaatagat accatcttta
     6781 cctgagtatc taatttgctt tgtgtcaaac attgttgtta ttcctattct gttaagcgtg
     6841 agctacgtga aatctaattt ttgatgttgg tatctgttca gaagatttca cgtagcattc
     6901 gactattcat agttgaatcg tcatactaga taaagattaa tagattgcca tgtgtggcaa
     6961 taaatattag tagctctaac attgtttatt cctctggata agctgagcta atcaaggtat
     7021 cttcctgcat acgattaata tgcgatgtgg ttctttcatc aataaccttt ccatcaagta
     7081 ccgtctgagt tgtgatattg attgtctgag gctgaatatt tactgacggt ttgataacct
     7141 ttaaatcttg tgtgaatgtt tccactcgac ctttcagatt atttaatgct tcaggtgaaa
     7201 actgtggaac atcttttgat ttgccctgaa ccatagccca taaaacagcg gctaaagagt
     7261 tcatgtcacc agtcaatgct tggtctgtgg gttccacctt tgaccttaca actttatcaa
     7321 ttggattatc tgacttactc atctgacgtt catagaacgg tttgacaatg gcattaatca
     7381 catc
//
LOCUS       DAZRUF010000203         7314 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZRUF010000203 DAZRUF010000000
VERSION     DAZRUF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.204"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            137..1201
                     /gene="ulaG"
                     /locus_tag="ACHXOL_003404"
     CDS             137..1201
                     /gene="ulaG"
                     /locus_tag="ACHXOL_003404"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR6810307.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1309..2064
                     /gene="ulaR"
                     /locus_tag="ACHXOL_003405"
     CDS             1309..2064
                     /gene="ulaR"
                     /locus_tag="ACHXOL_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR6810308.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2061..2810)
                     /gene="yjfP"
                     /locus_tag="ACHXOL_003406"
     CDS             complement(2061..2810)
                     /gene="yjfP"
                     /locus_tag="ACHXOL_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR6810309.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2992..3321
                     /gene="bsmA"
                     /locus_tag="ACHXOL_003407"
     CDS             2992..3321
                     /gene="bsmA"
                     /locus_tag="ACHXOL_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR6810310.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3470..3745
                     /gene="yjfN"
                     /locus_tag="ACHXOL_003408"
     CDS             3470..3745
                     /gene="yjfN"
                     /locus_tag="ACHXOL_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR6810311.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3862..5487)
                     /locus_tag="ACHXOL_003409"
     CDS             complement(3862..5487)
                     /locus_tag="ACHXOL_003409"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR6810312.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5571..6734)
                     /locus_tag="ACHXOL_003410"
     CDS             complement(5571..6734)
                     /locus_tag="ACHXOL_003410"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR6810313.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6737..>7314)
                     /locus_tag="ACHXOL_003411"
     CDS             complement(6737..>7314)
                     /locus_tag="ACHXOL_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR6810314.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENCYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
BASE COUNT         1909 a         1955 c         1797 g         1653 t
ORIGIN      
        1 cacactcaaa atcaacaaca acttccctgg caaaaagtca aaaaatggtc attgccttcc
       61 tgaaaaaata gtgtcaggat taatcaaaac caatcacata ttgattcgaa ttgaactatc
      121 atgaaggtaa atggcgatga gtaaagtgaa aagtatcacc cgagaatcct ggatcctgag
      181 cactttcccg gagtggggta gctggctgaa tgaagaaatt gaacaagagc aggtcgcccc
      241 tggcacattt gcgatgtggt ggctaggctg caccgggatc tggttgaaat cagaaggtgg
      301 caccaacgtt tgcgttgatt tctggtgcgg cactggcaaa caaagtcacg gtaacccgtt
      361 aatgaaacag ggtcaccaga tgcagcgcat ggctggcgtg aaaaaactgc agccaaacct
      421 acgcaccacc ccgtttgttc ttgatccgtt tgcgattcgc cagatcgacg cggtactggc
      481 gactcacgat cacaacgatc atatcgacgt taacgtcgct gctgccgtga tgcagaactg
      541 tgctgatgac gtaccattca ttggaccgaa aacctgcgtt gatttgtgga ttggctgggg
      601 cgtaccgaaa gagcgttgca tcgtggtcaa accgggcgat gttgtaaaag tgaaagacat
      661 tgaaattcat gcgcttgacg ctttcgaccg tactgcactg atcaccctgc ctgccgatca
      721 aaaagcggct ggcgtactgc cagatggcat ggacgatcgc gcagtaaact acctgttcaa
      781 aacccctggc ggttccctat atcacagtgg cgattcccac tactctaact attatgcgaa
      841 gcacggtaac gaacatcaga tcgatgtggc gttaggatcg tatggtgaga acccgcgcgg
      901 tattaccgac aaaatgacca gcgccgatat gctgcgtatg ggtgaagccc tgaatgcgaa
      961 agtggttatc ccgttccacc acgacatctg gtcaaacttc caggccgatc cgcaagagat
     1021 ccgcgtgctg tgggagatga aaaaagatcg tctgaagtat ggcttcaagc cgtttatctg
     1081 gcaggttggc ggcaaattta cctggccgct ggataaagac aacttcgagt accactatcc
     1141 gcgcggtttc gatgattgct tcactattga accggatctg ccgttcaagt cattcctgta
     1201 atctgtcgtt tataccggat agcgtggaga agttcgcgct atccggcgta gtatttccta
     1261 gctatttcaa atatcatcta aaaaaatcaa attttatcgg aatagctcat gactgaagca
     1321 caaagacatc aaatcctcct ggaaatgctc gcacaattgg gctttgtgac cgttgagaaa
     1381 gtcgttgagc gtctgggaat ttcgcctgcc actgcgcgac gcgatatcaa taaacttgac
     1441 gaacgcggca aactgaaaaa ggtgcgcaat ggcgcagaag ctattaccca acagcgcccg
     1501 cgctggacgc cgatgaatct gcatcaggcg cagaatcacg atgaaaaagt acgtattgct
     1561 aaagcggcct cgcagctggt taatccgggc gaaagcgtag tcatcaactg cggctccacc
     1621 gcgtttctgc ttgggcggga aatgtgtggc aagccagtgc aaatcatcac taattatcta
     1681 ccgctggcaa attacctgat cgatcaagaa catgacagcg tgatcattat gggtggacag
     1741 tacaacaaaa gtcagtccat cactttaagc ccgcagggca gcgaaaacag tctctatgcc
     1801 gggcactgga tgtttaccag cggaaaaggg ctgaccgcag aagggttgta taaaaccgat
     1861 atgctgacag caatggcaga gcagaagatg ctgagcgtgg tagggaaact ggtggtactg
     1921 gttgatagca gtaagattgg cgaacgcgcg ggaatgcttt ttagccgtgc cgatcaaatc
     1981 gatatgctta tcaccggcaa aaatgctaac ccggaaatcc tgcaacaact ggaagcgcaa
     2041 ggggtcagca ttctgcgtgt ttaaagatgc tggcggaaaa atgtcacggc agcatctaac
     2101 gcctcaggcg taatgcggtg acgcacgcct ggctgccatg aacaggttag cagtttatcc
     2161 cgtcccgtct cgcttaaggc ctgctgcaaa cgtagtgatt cgtcggcagg cacaacgtca
     2221 tcgtcgaggc catgccacag cagtagaggt ctgtccccaa gttgttccag gtggtttgtc
     2281 gcttcccact ctgccagcgg cgcgataatg ttattgaatt cattctgctg tgctgccgtt
     2341 tcaggtatca gcggtggaaa cagtgaacgg gcgagcgatg taaaatagcc cgatcccatc
     2401 atgctggcgg tacatttcac cgtggggtgg cgagcggtaa ttcccagtgc cgtcatcgcg
     2461 cccatcgacg cgccaccgac tgccagacgg tcatcaagca gccagttttc ttcggctatt
     2521 gccgcacgta aagtagtgaa ttcctgcata ctttgtagca agatttgcca gaattgattt
     2581 aaccgccgcg cttcgtcgcc actaaaacgg ctaccgtgat cgggcgcatc cggcatgatc
     2641 acccgcaaac cagcctgcgc cagcgcaacg gcaaaatagc tatacaccag gctggatgaa
     2701 gtaaagccgt gataaaaaat tacgcacggt aacggggtat ctttttgccc gacaggataa
     2761 gcatgaagaa cgggaatatc tgccagctcg cgtgattcta tttcaatcat ctgtctctcc
     2821 tttttgtctg gtatgacatg aagaaatcct tagcacaagt ttctgctggc ggtattagtt
     2881 cataatgttg agatgtgggt tacgctttcg tcacgttttc attcgaaaat cgcgtaccag
     2941 gtaacaattc gggaacattc ccccaaagtt aaattaataa ggcactacac tatggttagc
     3001 aggaaacgta atagcgttat ttaccggttt gccagtttat tattggtgtt gatgttaagt
     3061 gcctgtagcg cactgcaagg tacgccacag ccagcaccgc cagtaacgga ccatccgcaa
     3121 gagattcgcc gcgaccagac gcaagggtta cagcgaatag gtagcgtaag cacgatggtt
     3181 cggggttctc cggatgacgc attagcagaa atcagagcaa aagctgtcgc tgcaaaagct
     3241 gattattacg ttgtcgtcat ggtggacgaa accatcgtga caggacagtg gtattcacaa
     3301 gccattttgt accgtaaata aatcagacaa cttacatagt cagctttaca ttgctttgcg
     3361 tccctatgcg tttacctgtg cacaataaat taccggctgg aagcctgggc gtttattacc
     3421 gcgatggaat gccctgcaac atgtggggaa acgaaaaatg gagctgacga tgaaacaatt
     3481 acttgcctca ccctcgctgc aattagtgac ttatcctgcg agcgccacgg cacagtctgc
     3541 agaattcgcc agtgctgact gcgtgacggg cctgaatgaa ataggtcaga tctcagttag
     3601 caatatttca ggggatccgc aggacgtgga acgtatcgtg gcgttaaagg ccgatgaaca
     3661 aggtgcgtca tggtaccgca ttattaccat gtacgaagac cagcagcctg ataactggcg
     3721 cgtacaggcg atactgtatg catgagtgtc atctacagca aatgaacaat gccggatgcg
     3781 gcgcgagcgc cttgtccggc ctacaatttt cacatatttc attgagttaa tcgaaaccag
     3841 cgtcgcatca gtcgtatacg tttacaaaca cactcccccc gtcgcccgca gcaataaatc
     3901 attctggatc tgctctgaca accgtacgcc gccgcgcgta tctaacatca cctgacacca
     3961 cgcctgcgcc attggcggag aagcatattt caacatttgc gcaccgcagc ccagcaggaa
     4021 tagctgatga gtaatctctc gccccagttc ttcagctggc ttacgcagct gctgctgtaa
     4081 acgacgaacc gcgcgatcaa aatagcgatc ctgccctttc acttccacaa atgcttccga
     4141 caataagtcg tatacgcccg cttgcttatt gagaacgcgc aacacatcca ggcacataat
     4201 attgccggaa ccttcccaaa tactgtttac cggcatctcc cggtaaagcc gcggcagctc
     4261 gctctcctcg caataaccaa tgccgcccag cacctccatc gcttcggcca caaacggcat
     4321 acctcgtttg cagatcacaa attttgccgc aggcgtaaat aaacgcgccc acagggcttc
     4381 tttggcatcg gcgcgccggt cccacgctcg cgcaagacga aacagcaacg ccgtttgccc
     4441 ttcaagctga agtgccatgc gacttaagac atgacgcata aggggctgtt ggatcaatgg
     4501 attaccaaaa acatggcgtt gatgtgcatg ataaatcgcc agcgaaaacg cacggcgcat
     4561 catggcatgg ctacccaggg cgcaatcaaa acgcgtcatc ccccccattt tcaggatcag
     4621 acgaattcct tccccttcct gccccaataa ccagccaatg gcatcctgaa actccacttc
     4681 acaactggcg ttagagcgat tacccagctt atctttcagc cgttcgaggc gaatcgcgtt
     4741 gcgttgcccg tcaggcaaaa agcgcggcac aaaaaaacag gacagaccgc ccgtggtctg
     4801 cgccagcacc agatgcgcat cgctttgcgg aaccgagaaa aaccatttat gccccaccag
     4861 ccgataagag ccatcttcca aacgctctgc acgggtggtg ttgctcatga catcggaacc
     4921 accctgcttt tccgtcattc ccatgccaat caacaaaccg cgtttttgcc cacctggcaa
     4981 taagtgagaa tcgtagcgat cgctcagcag cggcgtggtc cagtcctgaa acggcgtggg
     5041 taacatctgt aacaacaatg gcgtggcggc aaaggtcatc gttattggac ataacgaccc
     5101 agcctcaacc tgcgcatgta acataaaacg cgccgcgcgc gccacaaatg cgccggagcg
     5161 agcgtcttct tcccaggcaa gattgtgcac ccgattggta cataacacct gcatcagcag
     5221 gtgccatgcg ggatggaaac gaacatcgtc cagacgccgc ccctgggcat cgtagcgcaa
     5281 cagttcaggc gggtttacat tcgccagccg ccccagttca agggattcag ccgttcctaa
     5341 ctgctgacca atactggcta gaaaatcgct atcccagcca gcaccttcac gcgttaccgc
     5401 ttcgcaaagc gcgccatcag acaggtataa gttgctgtta tttaatggta taggttgatt
     5461 aaaaacggtg tgagtttgcc agtgcactgt gtctccctcc atcaatggca gtcaccatta
     5521 gtatggccac tgccatggat tcatgacaga aagagattgc taaaacattc ttatccagca
     5581 atgtaatgcg gaacgaaacg tgaggtgtct ttggtgatca gtgtgttatc ttcacggatc
     5641 cccattccgc acgcttcatc atcgacaatc cagctatcga tgagtgtgta gctatcgcca
     5701 aatcgcggca gaggttgaaa cgcctggtag atcatcggtt catcggcgta atcaccatca
     5761 gcgtggtcaa cgacgttatt ctgaccgtcg aaaatggtga cgtttccgcc ttcgcgcgag
     5821 tagatcggtt tacgcacata gctttcgcca gcggcaatct gcgatttttc accctcaaac
     5881 caggatgcaa gaaggttagg atgaccaggg aagaagcgcc aaagcagcgg cattagccct
     5941 ttattactca agatactttt ccataacggc tccacccatt gctcgcgacg cttgcacagc
     6001 agcggaccgt tatcgtcacg catcatccac tccagcggat acagcttaaa tgcacgctgg
     6061 atgacattat catcaagatc ggtcagtaca ccgccgacgc ccaaaccgag atcttcaatg
     6121 tagataaacc gcgactcctg ccctgcctgc tgggcgcagt cctgcaagta cagcacggta
     6181 gtcctgtctt catcggtgtc ctgacagcag cagaaataaa acggctcccg gctataaagc
     6241 tcgctaaagc gtgaaatcag gcgttcctga atggcattgt actgatcggc atcacgcgga
     6301 ataatgccgc tgcgccgggc atcctccagc cacaaccact ggaaataagc cgactcatac
     6361 aacgaagtcg gcgtatcggc gttgtactcc agcagcttca ccggcgcatt gccacaccag
     6421 gcaaaatcca tccggccata cagcgaagga tcgctggcgt gccagctttc ggcgatcaca
     6481 tcccagtaca acggcgggat cgccagttgc gtcaggatct cttcgtcttt aaccgcgcga
     6541 tccaccacct caaggcacat ctgatgcagt tccgcagttg gtttttcgat ctgctcttca
     6601 atctggcgca gagtaaaacg gtaagcccga ctctcgtccc aatagatttc attgtcgatg
     6661 atatgaaagt cgaaaccgtt gtcagcggca atttggtcca gatcccgccg cacaggaacg
     6721 ttgtgtctca gcatgattag cctccccaat gcccacgggc gctggaagaa cgaccatagc
     6781 cgccgcgcga aacggttgac gcttttttgg tggtaaagcc gcctgaagag taagactctt
     6841 ttttgccaga accggagcgc caggagtaat caccggaagt gttgcgccag acggggcggg
     6901 aagcaaatga ggatccgccg ctgttataga cgaacggctc atcgcgatct ttacgaataa
     6961 cccggcttaa caaaaatcca gaaacgaccg ggatccaact ctgttcaaca ttgtcgtagt
     7021 agcaattttc gtacttagac tgacagttct gctgagtcat attcttcgga acatcggcat
     7081 aaaatgccgt tttggcgttg ttccagccac gcgcgcaaat gtcggcatta ttaccgtcat
     7141 caatacaatc ctgcactgtc gcgtaaaacg ttccgtcgcc gtcgttatcg acatcgctac
     7201 tatcgccaca acctttcaat acgaaaaaag cggcaccgcc cattagcgcc aacgttaagt
     7261 atttttgtgc gtagcgattg cgacgcggtt caaaaggatt attcggtcta ccaa
//
LOCUS       DAZRUF010000204         7299 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZRUF010000204 DAZRUF010000000
VERSION     DAZRUF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.205"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            180..422
                     /gene="yiiF"
                     /locus_tag="ACHXOL_003412"
     CDS             180..422
                     /gene="yiiF"
                     /locus_tag="ACHXOL_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR6810315.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(752..1681)
                     /gene="fdhE"
                     /locus_tag="ACHXOL_003413"
     CDS             complement(752..1681)
                     /gene="fdhE"
                     /locus_tag="ACHXOL_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR6810316.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1678..2313)
                     /gene="fdoI"
                     /locus_tag="ACHXOL_003414"
     CDS             complement(1678..2313)
                     /gene="fdoI"
                     /locus_tag="ACHXOL_003414"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR6810317.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2310..3212)
                     /gene="fdoH"
                     /locus_tag="ACHXOL_003415"
     CDS             complement(2310..3212)
                     /gene="fdoH"
                     /locus_tag="ACHXOL_003415"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR6810318.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3225..6275)
                     /gene="fdnG"
                     /locus_tag="ACHXOL_003416"
     CDS             complement(3225..6275)
                     /gene="fdnG"
                     /locus_tag="ACHXOL_003416"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5688..5690),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR6810319.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6469..>7299
                     /gene="fdhD"
                     /locus_tag="ACHXOL_003417"
     CDS             6469..>7299
                     /gene="fdhD"
                     /locus_tag="ACHXOL_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR6810320.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1636 a         1941 c         1966 g         1756 t
ORIGIN      
        1 tcatcctggg cgataactaa accatttttg agtaataaat gaaacaactg caacgctctt
       61 attatttctg gaaaacgcct cgcaaaaata aaaaatgatg cgtaaatgag ctttctttaa
      121 agattcggca agggtacttt tctctccata gattcaaaaa tgatattggc gagatattta
      181 tgaactctct ggcaggtata gatatgggca gaattttact cgatttatcg aatgaggtga
      241 ttaagcaact tgatgatctc gaggtgcagc gtaatcttcc tcgtgcggac ctattaaggg
      301 aagcggtaga tcaatacctg ataaatcaat cgcaaacagc aagaaccagt gttcctggca
      361 tctggcaagg gtgtgaggaa gatggtgtcg aatatcagcg taagctgcgc gaggaatggt
      421 aatgggaaaa aatgatgtca atcagattgc tgacaacgtg cgcgttgttc atgccggatg
      481 cggcgtgaac gccttatccg gcctacaaag tcatgcaaat tcaatatatt gaagtttatt
      541 ggtaggcctg ataagcgcag cgcatcaggc aatcatacgc ttgtcatcag tgacataccc
      601 ataaccttat cgatgtgttg gagggaagaa cgaggcaaac agacaattgc ggcatgatct
      661 tgaatgggta acgcggaata cgagagatgg tgtcgtcaca ccctatgagc tgtaatccgg
      721 gcgacctgcg ccacccggaa aacgtcagag attactctcc ttcacccgga aacaggaacg
      781 ggttaatgga actacgggcg tagccttctt gctccattcg cgcgtccagt accagagagg
      841 cgaggtcatc tgccacggct tcaactttcg gttctttttc ctgataaaga atcttcaggt
      901 aagtgccgca gtcatcgcag ctttcggctt taatcgcggc ctgttcgtca tccagcgacc
      961 agtaatgcag tttgccgctc tgctcgcagt tgctgcattt tacgcgcact acgtgccatt
     1021 cggtttcaca caggttgcag tgcaggtaac gcagaccctg agtggtgcca atttgcacca
     1081 tgctggacac cggcatagag ccacataccg ggcaatattg acgttgttcg ccgtattcag
     1141 cgcgggcttt gccggggatc agattggcca tctgcgccca gtagagcgac agcgcagccc
     1201 agataaacgg cgctttatcg ctgctgacgg acgagaaatc agaggcaaac agcgcgctgg
     1261 ccatatcttc cagctcctga gtcgatgcct tctccagatt ctcaatcact gccagcgccg
     1321 ggccgctcat ttcaggtttc agctcagcaa ttagcgccat cagcagcttt tgccagtgct
     1381 tatcacgcgg cagaacgtga atatccagcg ggggcttgcc ctgtgcgctg gcttctttaa
     1441 tgcgcgcggt cagatccatc tccagcggat ggtcgtacag caccacttcc tgggcgtggg
     1501 cgataagcgc agcaaagcgc aggtaatcac ccagcggatt attttctgcc agctcgcgca
     1561 gacgctcggc gcggcggttg tataaattct tgagccgagg gaacaataac ggcggaatca
     1621 tatccgccgt acgtttctcg ctcgaaccca gctcatcttg cgggattatg cgaatactca
     1681 ttcagctttc ttttccgttg tcttgcggac ctcacggtac cagcgcgggt gatgtttctt
     1741 cgcccaggcg ctggtaaccc atccttccac catggcggta atcgtgcctt tcacccaaag
     1801 ggcggcgtag atatgcacca tgataaccac aattaacgcc actgcggcaa atgaatgcag
     1861 cattaacgcg aatcggatca ccgggattga gaaagcaggc gcaaaataag gacgccagat
     1921 aatcacgccg ctcaccagca gcagaaccag gaaaataatc gccgcccaga aaacgcattt
     1981 ctgaccgaag ttataacgcc cggtgtcacc tacttcctcg ttgacgacga tcttacgaat
     2041 attcttcgcc caaaagatat catcccgatt gattaggttg tgatgccagt aacggaaaaa
     2101 catgatgatg aacgaggcaa acataaccac gccgacaaac gggtgcagaa ttcgcgccag
     2161 ctgcggtgtg cccatgattt gcatcaacca gttgaaggac gggaacaaaa agcccagccc
     2221 gctcaccgcc gccaggatga agcagaaggc ggtgatccag tggttgatac gttccggcgc
     2281 ggtgtagcgc acgatggtgt cacgtcgttt catttgcgct cctcgtcttt ctcttcgtgc
     2341 agattattct cttcctcatc cgcacggttc ggaccgacac ccacgtagtg gaagatactg
     2401 gccgcaaagg tagccgcaaa gccaacagcc gcgagcggtt tccagatgcc tttccagaat
     2461 ttcacggttt cgctgatttc cgggttctcc ggcaagccat gatacagatt tggcttgtca
     2521 gcatggtgca gcacgtacat gacgtgtgta ccaccgacgc ctgccggatc gtacagaccc
     2581 gcattgtcgt aaccgcgggt tttcagctca gcaacgcgct cgctcgccag cgttttcatc
     2641 gactctttcg taccgaagtg aatcgcgccc gttgggcagg tcttcacgca ggctggttct
     2701 tgcccaacca ccacgcggtc aacgcacagc gtacatttgt agacgcggtt gtcttccggg
     2761 ttgaggcgcg gaatgtcgaa cggacagccc gcaatgcaat aaccgcagcc aatgcactgc
     2821 tcggactgga agtcgacgat accgttggca tactgaataa ttgcaccttc cgccgggcac
     2881 gctttcaggc agcctggatc ggaacagtgc atacagccgt ctttgcggat cagccattcc
     2941 agtttgtcgt tctgctccac ttccgagaag cgcatcaccg tccacgattt ggcgcttaaa
     3001 tcattggggt tgtcgtacac cccaatgtta ttgccgacgg tatcgcgaat gtcgttccac
     3061 tctgaacacg ccacctgaca ggctttacag ccgatacagg tggtgacgtc gatgagtttc
     3121 gccacttctt cctggaagtc ccgcgcctga ggcgcggggg tcagaccgtt agtcgcggaa
     3181 cgacgaatga tatcttgcga ttgataagcc atatgtcgtc tccgttacac cttttccaca
     3241 ttcacaagga aggacttaaa ctccggcgtc tgcgtgttcg catcaccaac gaatggcgtt
     3301 aacgtattgg caataaagcc ttttttagct acgccttcat agccccagtg aatcggaata
     3361 ccgatggtat cgatatcttt gccgttcgct ttcagcgtgc gaatacgttt ggtcaccacc
     3421 gctttggctt tgatatagcc acggttggag gagactttca cggtatcgcc ctgggcaatg
     3481 ccaagtttat tcgccagcga ctccccgatt tccacaaact gctctggttg caaaatcgcg
     3541 ttcaacagcg cgtgtttggt ccagtagtgg aagtgctcgg tcagacgata ggtggttccg
     3601 acatacggga acttatcggc tttacccaat gcttcggcgt cgtctttaaa gattcgcgca
     3661 gccgggttcg agataacgtt tggatgcagc gggttagttc ccagcggcgt ttcaaacggc
     3721 tcgtagtgtt ccgggaacgg accttccgcc atcttatcga gggcaaacag acgccccatg
     3781 ccttcctgct gcatgataaa cggcccgacg ccgctgcccg gaggcgctgc gctgtagtcc
     3841 ggaatatccc agccggtcca cttagtgccg tcccatttca gcaactgacg cttcggatcc
     3901 cacgggttac cctgcggatc tgcggaggcg cggttataca gaatgcggcg gttaagcggc
     3961 catgcccatg cccagcccag cgtgttaccg aggccagacg gatcggcgtt atcacgacgc
     4021 gccatctggt tgccttccgg cgtccagcta ccggcgaaaa tccagcagcc acaggaggtt
     4081 gtaccgtcat cgcgcaattg ggcgaacgag ctaagttgtt ggcctttctt gacgataacc
     4141 gcaccggttg ccggatcggt aatatcggcc agcgccttac cgttgctctc catcgccact
     4201 tcttccgact tcggctcatg cggaatggcg tagttccagg tcatgttcag cacctgatcc
     4261 gggtttgcgc caccctgttc ggcatacatc ttgcgcaagc gcaggaagat accggagagg
     4321 atctcgccat cagtcagcgc aatccctggg gcgtccgcac ctttccagtg ccactgcaac
     4381 cagcgacctg agttaacgat tgaaccgttc tcttctgcga agcaggtcga tggcagacgg
     4441 aacacttcgg tctggatctt cgacgagtca acttcgttca gctcaccgtg gttctgccag
     4501 aagttagagg tttcagtgtt cagcgggtcg atggtgacga ggaacttcag tttcgacaga
     4561 caaccgatca ctttgttttt gttcgggaat gaggcaacag ggttaaagcc ctggcagata
     4621 tagccattga ccttgccctc tttcatcatc tcgaagtatt gcaggacgtc gtagccttta
     4681 tcccacttcg gcaaccagtc aaagccccag ctattttccg ccgtcgcttt atcaccaaag
     4741 aaggccttca tcatagagac gaagaatttc gggtagttgc cccagtagtt tacctggcct
     4801 tccagcagcg gttttggcgt gttggcggta aggtaggttt gcagatcggt ctgcttctcg
     4861 cttggcagcg tcatgtaacc tggcaggctc tgcgacagca gccccaggtc cgtcagcccc
     4921 tgaatattgg agtgaccgcg cagggcgtta acgccgccgc ctgccatccc catattgccg
     4981 agcagcagct ggatcatcgc catcgtacga atgttttgcg caccaacgga gtgttgcgtc
     5041 cagccgaggg catacaggaa cgaggcagtt ttatcgtgag cactggtttc tgcgatgtat
     5101 tcgcagactt tcaggaacgc gtcttttggc gtaccacaga tgttttcaac cacatctggc
     5161 gtgtagcggg aaacgtgctg tttcagcaag ttccacacgc agcgcggatg ttgcagcgtg
     5221 gtatcgcgtt tggcgaagcc gttttcgtcc agttcgtaag tccaggagga tttatcgtac
     5281 ttgcgttttt ccgcgtcata gccggtgaac aggccatctt caaagccgta atcctcacgc
     5341 acgatcaggc tggcgttggt ataggcttcg gtgtattcgc ggttgaattt ttcattgttc
     5401 agcaggtaca gcaatacgcc tgacaggaaa gcaatgtcag taccggaacg aataggggca
     5461 tagtagtcag ccaccgccgc cgtacgcgta aagcgaggat cgatcacaat cagcttcgcg
     5521 ccattgtgaa ttttggcttc catcgcccag cggaacccga ccgggtgagc ttcagcggcg
     5581 ttaccgccca tcaccacgac gaggttggcg ttcttgatgt cgacccagtg gttggtcatc
     5641 gcaccgcgac caaatgttgg agcaagactt gctaccgttg gtccgtgtca gacacgcgcc
     5701 tggttgtcga ccgcgagcat acccagcgcg cgggagaatt tttgcgttaa atagccggtt
     5761 tcgttactcg acgcggaagc acacagcatc ccggtggaga gccagcggtt aacagtcacg
     5821 ccttcggcgt tttgcgcaat gtagttagca tcgcggtctt ctttcatcag tttggcgatg
     5881 cgatcaaacg cctcttccca actgatttgc tgccatttat cagaacctgg cgcacggtat
     5941 tccggaaact tcagacggct ttcggagtgg atgaaatcca ccaggccagc gcctttcgga
     6001 caaagtgcac cgcggttgac cgggtgatcc ggatcgcctt cgatatggaa gatagatgct
     6061 ttggcgtttt ttgctccgtc accgaggctg tacatcaaca gcccacagcc tacggaacaa
     6121 taggtgcagg tattacgggt ttcgcgggtg cgcagcagtt tatactgccg ggtttccgcg
     6181 agtgctacgc tgggtgcaaa acccagtgcc gctgccgtgg tgcctgccat accgccagcg
     6241 cagatcttaa agaactgcct tctgctgacc tgcatggatt gctccttgtt tcgacattgt
     6301 cacgtcccat ttacattcgc ttgctgcgtg tgcagggagt gggagttatt tttctttgcg
     6361 gaaggggccg caaaggtcca gaattggctc aatttccctc catccaggaa gggtttgtaa
     6421 cagaatacca taatgttggt gtgtgtgttc ttatctggtt aagagaaagt gaaaaaaaca
     6481 cagcgaaaag aaatcgaaaa tgtgacaaat atcacaggtg ttcgtcaaat tgagttatgg
     6541 cggcgtgatg atttacaaca cccacggtta gatgaggtcg cggaagaagt tcccgttgcg
     6601 ctggtctaca acggcatttc gcatgtggtg atgatggcgt cgcccaaaga ccttgagtac
     6661 tttgcgctcg gtttttcgct ttccgagggg ataatagaaa gtccgcgcga tatcttcggc
     6721 atggatgtcg ttccttcctg taatggtctt gaagtacaaa ttgagctttc cagccgccgc
     6781 tttatggggt tgaaggagcg ccgccgggcg ctggcgggac gtacgggctg tggcgtatgc
     6841 ggcgtggagc aacttaatga catcggaaaa ccggtgcagc cgctaccgtt cacccagacg
     6901 tttgatctca acaaactgga tgatgcatta cgtcatctca acgattttca gccagtgggg
     6961 caactgactg gttgtactca cgccgctgcc tggatgttgc catctggcga actggtcggc
     7021 gggcatgaag acgtgggtcg ccatgtggcg ctggacaaac tgttaggccg ccggtcacaa
     7081 gaaggggaaa gctggcagca aggtgcggta ctggtttcca gtcgtgccag ttatgaaatg
     7141 gtgcaaaagt cggcgatgtg cggcgtagag attttgtttg cggtgtctgc cgcgaccacg
     7201 cttgctgtag aagtggctga gcgctgtaat ctgacactgg taggtttttg taaaccgggt
     7261 cgggcaacgg tttataccca tccgcagcgt ttgagcaat
//
LOCUS       DAZRUF010000205         7288 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZRUF010000205 DAZRUF010000000
VERSION     DAZRUF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.206"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..991)
                     /gene="pyrD"
                     /locus_tag="ACHXOL_003418"
     CDS             complement(<1..991)
                     /gene="pyrD"
                     /locus_tag="ACHXOL_003418"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR6810321.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIK"
     gene            complement(1102..1839)
                     /locus_tag="ACHXOL_003419"
     CDS             complement(1102..1839)
                     /locus_tag="ACHXOL_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR6810322.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(1805..2320)
                     /locus_tag="ACHXOL_003420"
     CDS             complement(1805..2320)
                     /locus_tag="ACHXOL_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6810323.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2328..2870)
                     /locus_tag="ACHXOL_003421"
     CDS             complement(2328..2870)
                     /locus_tag="ACHXOL_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6810324.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2882..3952)
                     /locus_tag="ACHXOL_003422"
     CDS             complement(2882..3952)
                     /locus_tag="ACHXOL_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6810325.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(3943..6542)
                     /locus_tag="ACHXOL_003423"
                     /pseudo
     CDS             complement(3943..6542)
                     /locus_tag="ACHXOL_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(6567..7268)
                     /locus_tag="ACHXOL_003424"
     CDS             complement(6567..7268)
                     /locus_tag="ACHXOL_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR6810326.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
BASE COUNT         1844 a         1875 c         1630 g         1939 t
ORIGIN      
        1 ctttaatcag cggcggacct ttaaaaataa aaccagaata aatttgcacc agtgaggcac
       61 ccgcagcaat cttttcacgc gcagcgataa ccgagtcgat gccgccaaca ccgatgatcg
      121 gtaagcgacc gtttaattcc agtgacaagc ggcgaataat ttcggtgctt tttaactgaa
      181 gcggacgacc acttaagcca ccggtttgat cgcaattttt cattccctga acaagagaac
      241 gatcgagtgt ggtattggtt gcaataacgc catcaatatt atggcgaact aaactatcgg
      301 caacctggat caattcttct tcagaaagat ccggcgcgat cttcactgcg atcggcacat
      361 atttatggtg catcgcttgc aaatcatttt gcttattttt aatcgcggtt aagagatcat
      421 ccagcgcttc accatattgc agcgtgcgta atcctggggt attcggcgat gaaatattga
      481 tggcgatata tcccgcatag gcatagattt tttccataca aatcagatag tcatctttgc
      541 cctgctccac tggcgtatct ttatttttgc cgatgttaat acccaggacg ccgtcataat
      601 gggccttttt tacgttctct acgaggttat caacgccaag attattaaag cccatacggt
      661 tgatcaaacc ttcggcatct accagacgaa agagacgcgg cttgtcatta cctggctgtg
      721 gacgtggcgt gacggtaccg atctcgatcg atccaaatcc catcgcgcct aacgcgtcaa
      781 tgcactcccc gtctttatca agaccggctg ccagaccaag cggattttta aacgtcaggc
      841 ccatgcagtt aacaggtttc gcaggcactt tctgccgcac cagtgcttca aacggcgttc
      901 ctgtaatacg gcgtaattgc tgaaaagtaa actcatgagc gcgctctgga tcgagctgga
      961 aaagggcttt acgaacgaag gggtagtaca tgaactctcc tggattcccg gtgtgcaaac
     1021 cgggggcgta ttatgtgcga gttcggacca aaagggaatt gacctgcggc aaaaaaaacg
     1081 caaacgtttt ccgaacctgt ttcagggcag attttgctcc ttctttgcac tcaacccgcc
     1141 gtaatcattt atcgcccgcc aggcgacatg acacggtgcg attgcctgac accagcttgt
     1201 ctgacgctgg cttttaggcg gcacaactcc cgcattactt aacgctttac catttacgct
     1261 cacctgaatc aggttaatgt agtaaggcgt tgggttagtg agttgtacac cctgcgaatt
     1321 ccgtgtccag cgtaattgtt gataagcttc cagcgggtcg cccggcaaac cttcgggtcg
     1381 ccagaacagt ttaaataccg agcgcatcgc cacttttacg ctctgatttt caactttgcc
     1441 ggatggcacg ctggcaatgc ttaactcaaa tagcgtttcg cgatccacag gcaagatgtc
     1501 gctttccgtt ctcagcaagc gcaacgtgcc tgtagtaccg ggcttcagga gaataagtgg
     1561 cggagcggcc agtaatggtg ctggcactgt cgacgcttcc cccccggccc aacgcgttgg
     1621 gcggttgatt ttactgttaa taagccagga ttcctgactg gtattagtca gtaaaataga
     1681 tatcgattct ctgtctgccg gaaagataaa tcgcgttccg ccgaccacca ctcctgcccg
     1741 caattcaccc gccaccagca gcgataaaac ggcgaaaatc aagagcgcca atctgttcca
     1801 cgtattagtc ataatgaagt acaaatgtcg cgctggcatt cactttaccc gactgcacat
     1861 cgctattgac aggtcgcaat tgcgcataaa aagttaatgg caccttaccg ctatgtaggc
     1921 tgtagtcttt actcgcctgg ttaagcggaa tcaatatctt cgcatcatcc agaatcgcaa
     1981 ttgccagccc gctggctgca tccgtaccac tttcaatgga cagcatttcg ctctgcactc
     2041 cgtcgggggt tcctgaaaag gtgacgtcaa ccgtgctgac atctgttccg caatcctgca
     2101 aattaatgat gaaagatttc tccgggccat aattcccatg aaaaaaacgt gtcagcgaga
     2161 catcgcctaa ctgcacattg atttgcttgc tatcctgtgc cagcacgcat gtattacggc
     2221 gaaacgatcc gctaatgttg accgtgccat catgggcaaa tagctcacag ccccccgtta
     2281 atcccagtaa gaacagaatc cagattatcc gtttcaacat cactccttca ctggtaaacc
     2341 agatcaaaat agagaaccgc cgtcgcatta ccgcccgttg ctcccgcctt tgtggactta
     2401 taacgaagtt gatatttgag tgtgttatcc ccggctttta agggcgtaag aggctggacc
     2461 tgattgagcg ggatttcttg ctgactttgc gcatcaagaa tttgcacggc aatccccgtt
     2521 gccataccac cgcttccgcc tgtgtcagtt agtttgagca gtgtcggcgt aagggtgtca
     2581 gcctcgccgc taaaaaggac gtccgcgcca gtaatactag cggcacaacc ggtgatatcg
     2641 atattcaaat cagcagcagt gcttacggta ttcgcggcct gaaaatcact gatattaaaa
     2701 tcaccaatat gaatattttg taatgcactt ttggtggcga catcacagcc ctgatcgacc
     2761 acattcccat aaatatgcat actaacttgt tcaggcccag ccatcccgac atggatgtta
     2821 agaatgctaa agaacaagat aacgcactga aatatcgttt ttttcttcat acgttcacgc
     2881 gttaatcaaa atttattctc accgttgccg tggcttcaaa tcgcccggtt tctggtggta
     2941 cacctgtcgt gctgactgga tagacattca ttcgcgcgat actgtctgca tcatcaggaa
     3001 tagagagcgg taatgttcca ccagcagggg aaataatgtt gttctggcta tcggtcacca
     3061 ccacgcccac gccaggacgt gacgtcttaa tcgcctgggg atagctgggg tctgtggttg
     3121 ccgtcagact caggttgacg ttaactaacc ccgcgtttcc cgtgttacac ctgactggaa
     3181 ttgcaagttc tgccgttctg gccccaagag gcttatgccc tacaacacga aagttagccg
     3241 caaatgcgtc gcccagatcg acctcgattg tttctccggc attgacggaa catgagccaa
     3301 gtgaattgat cgtaccagac aggtaataca ctaaaaccgg tgtacctgta gtcgtgcagg
     3361 aatcacctgc gggtatgttg tagcattcaa ataatcgcgc cagcgccata ggaggaatga
     3421 cgaccgaacc aacgaacggc tgcaatattt tcagggaaaa gctaccgcta ttaccgctag
     3481 ccccctggcc acctaaacgt tgaccgccgg gatcgcaaat atgccatcgt gtatctgccc
     3541 ctgtgcccac gttttcgaaa ggaaccgcag taggagaact gcctttaacc tgcaataaaa
     3601 ctttggcggc aagatagtca ttaattttgt accagttgtc gcctaaatag actaacggtg
     3661 aatccgcggc gaatgcccat cggccacttc tgacattgtc tttatcgcaa tcacaggaaa
     3721 cataataatc attcgattcg ttccactgat aaaatttgtc tttaactact ccagccgtat
     3781 tttcttcttc ctccagggtt ttaacgccaa aattgacgcc aatatacgga ttcccctgag
     3841 tggtatgaca ctgcccgcta taagcagcag tttgagcgca ccatagcatt aagacggcgg
     3901 caaaaaatag tcgtagtaat aacacgcatt tttctccaaa gattatctgc atatggcatc
     3961 cattcgcgta atcgcagagt taatttgttg ttcggtaagc tggtaactga tatgacaaat
     4021 ttgatcggga gcttcgcccc agcgtacagt tatcgctccc tgagcgggca aaccggacat
     4081 atagagcgag ccatcatcat caataatgcc gctattagtc tgtacgccgt ccagcgtcgc
     4141 tatcgcgcca aaacgcagcg gtataccctg ctttgatgtt ttcattaata cccttgctcc
     4201 cgcatgggca ttgaactccg ccagaaccaa cgcccctttg gtgggaacca cgttgactac
     4261 tgcattttca agatccacgt tacgttttaa cgatgccgca tcaagtgcga ttcgattttc
     4321 ccgataagaa gtggcatagg ggacaacggt atatccgcgc cagtcagttt tcagcccggt
     4381 gctgttcgcc acacgagtat tctctgcgcc tggtgctgca atcagcacgt tagtttcccc
     4441 cagcgattgc cccagggtaa tgccctgtga atgggcaact aaactgcctg aaagagcgta
     4501 gttcagttgt tgttgactgc cgttatcgct gtagttgtag cccactcggg catctgcaaa
     4561 cgcccctttg tagtccatgc tggcgctacc attagccgtt ttcccctcgc tgttatatcc
     4621 ctgttgcacg ctgtagctca ggttaccgtc atcgagtagt gtctccgtta aacccaggtt
     4681 ttgtgtaatg tgcccttcgt tatcgatgct ggtgttagag gtcatccagg cgtaattttt
     4741 ttcctggtat agctatcatt acttctgggg agcaacagat tgagcggcag ggaaaaattt
     4801 agtgcaaaca cctgatcagc atcaggttga ccacgggact tactgtagtt ccaggaaccc
     4861 cccagcgaga gatctttaat ttgcgtgttg tagccaaact gtaatagccg atcctgttga
     4921 tcggtatgcc agtaagtttg ctggctacca cttaaataaa acgaaccgta ctcgcctaat
     4981 tgctgggaga tattgacctg cagtttgcca cgtttggtgt aaaacaaatt gtaataacgc
     5041 gaccacatcg gcgtatcttc atcatcaccg tcattaaatt cgtagccatc catatgttta
     5101 tacatggtgt cggaaagggt gtagaaaccc gacgtcgaat agcgatagcc catcaattga
     5161 aaattagtgc ccaattggtt gagcgttttg gcatacagaa aacgatatga ctgaccttta
     5221 tgttcgcttt ggtctgccag cgtactcttc gcctgggtcg catcgaacga aattgctccg
     5281 aaatcgccaa ggttaaatcc cagaccaaac atggcggcac ggtaatattc agcatattgt
     5341 ccaccaccat accatgtcgt tccccacggt ccgccccact gcaacgtggc ctaggcaaat
     5401 ttgctctcct gctgttcatt actattggtt ctgtatttcg ccagtgtcac cgcgtatttg
     5461 attcgcccct gacgctggag taatggcacg ctggaaaacg gtacgctgta gctattgacg
     5521 ctgccgtccg cttctttgat ttcaactaac aaatcaccgc tcgacgacgt ggaatagaga
     5581 tcactaattt caaaagcacc aggcgataca taagtttggt aaatggtgta accattttgc
     5641 ttaatcgtta tctgggcatt gcttttggcg ataccacgca ccacaggcgc aaaccctttc
     5701 aggctgtctg gcagcatgtt gtcatctgac gccagctgca caccacgaaa gctgacacta
     5761 tcgaaaaaat cgcctgcagt ataatcatca cctacggtca gttcgccttt tagtggaata
     5821 accgcccgct gcaaatagct gctgagattc ttccattctg cggtttggcc actactgcga
     5881 ctccatgttg aattgttgcg caatctccat gggcctaaat taacgccact gtttaaattc
     5941 agaaaatagc tgtcgccaga atcactgtct gcgctgctat gaatactgtt agccccgcta
     6001 aacgaatatc ccagtaatag cgcgttgatc ccttcatccc aacgttcagg ggggacataa
     6061 ccacgggcat tacttttgat ggcgatttgc ggtactgaaa tttccagacg gagtttattc
     6121 acattaaacg ttacgctggc atccggaata atctccgcca atggcacaca cgcttgtttg
     6181 tccagttgtt taaaagccgg aaacgcatca gtattaacac ccattgcatc caggctttca
     6241 gtcgtcaaac aggcggcaat ccccttctct ggcgttatct caacaaaatt gacgttgcgc
     6301 gtatctacaa ttgtctgatt cagaacaata tcgacacgat aaacccctgg cgttattttt
     6361 tgtccttttt cgaaacgaga caaatcggcc accattgagg gatcatcttt gaaaaaagca
     6421 ggatcgaacc aggtttcagc ttgtgcattg aacgctgccg acacaaacac caccagccca
     6481 ccaataaacg atggcactcc accagagctt aacaggctgt gttgtcgatg agttctatac
     6541 atctcgctct cttaactgaa gacggcttac gatttacagt tgagttgtgg cgtttccgcc
     6601 ccgtaatcat tgatggttcg atacgttacg ctgctatttt tggccccctt tggcagagag
     6661 aattggtatt tatcaaaggg ggaaatcatt tgatttttaa ctacctttcc ccctattttc
     6721 aggtctgtca gggtaatgaa ataaggtgtt gggttactga ccgtaaccat gtcagctgac
     6781 gaagtgcaac gcagtttcgc cggagcctcg cctaagggat acgctaaacc ttttggtcgc
     6841 cagaaaagtt tgatgacgct ttgtgtggca atcttcaggg tatttccctc ttcccgcttc
     6901 gttgttgaag ggactgcccg tacgctagtg aagaaaaggg tttctctgtc tttcgccaac
     6961 ggcgcaccaa tgtacataat gcgtaacaga ttctcattag ccggattcag cacaaatagc
     7021 ggtggtgtaa taataaaatc tgctgatttt ttattatctg gggtcatcac ccatgactga
     7081 ataagataag gaacatccgg actggtgtta cgtacaccca gtgaaatttg ctcttcacct
     7141 tctgaataaa caagacgtgt tgctgcaagt ccaatcccgc ctttacctgc cgcccatgcg
     7201 gatgagcagc tgagtaatat tgccgttaaa cttacggtca caattccctt tgttatgcaa
     7261 gttttcataa atagtcactc ttaaaaaa
//
LOCUS       DAZRUF010000206         7261 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZRUF010000206 DAZRUF010000000
VERSION     DAZRUF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.207"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            106..1407
                     /locus_tag="ACHXOL_003425"
     CDS             106..1407
                     /locus_tag="ACHXOL_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR6810327.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLY"
     gene            1674..3329
                     /gene="opgD"
                     /locus_tag="ACHXOL_003426"
                     /gene_synonym="mdoD"
     CDS             1674..3329
                     /gene="opgD"
                     /locus_tag="ACHXOL_003426"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR6810328.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            3469..3693
                     /locus_tag="ACHXOL_003427"
     CDS             3469..3693
                     /locus_tag="ACHXOL_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR6810329.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            3756..4292
                     /gene="rimL"
                     /locus_tag="ACHXOL_003428"
     CDS             3756..4292
                     /gene="rimL"
                     /locus_tag="ACHXOL_003428"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR6810330.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4287..5267)
                     /gene="ydcK"
                     /locus_tag="ACHXOL_003429"
     CDS             complement(4287..5267)
                     /gene="ydcK"
                     /locus_tag="ACHXOL_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR6810331.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            5391..6383
                     /gene="tehA"
                     /locus_tag="ACHXOL_003430"
     CDS             5391..6383
                     /gene="tehA"
                     /locus_tag="ACHXOL_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR6810332.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            6380..6973
                     /gene="tehB"
                     /locus_tag="ACHXOL_003431"
     CDS             6380..6973
                     /gene="tehB"
                     /locus_tag="ACHXOL_003431"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR6810333.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         1826 a         1707 c         1818 g         1910 t
ORIGIN      
        1 ataatattga tgttttcgcg gcgagatcac agtttgtaaa ttcttcccgc cagagtgaat
       61 gcggttacct acactccaga ttactgacca ctggaggcag acactatggc gaacagcatc
      121 acggcggatg agattcggga acagttttcg caggcaatgt cagccatgta ccagcaagaa
      181 gttccgcaat atggcacgct gctggaactg gtagctgatg tgaatctggc tgtgctggaa
      241 aacaatcctc aactgcacga aaaaatggta aatgcagacg agctggcgcg actgaatgtt
      301 gaacgtcatg gggcgattcg cgttgggact gcacaagagc ttgctactct tcggcggatg
      361 tttgccatta tggggatgta cccggtgagc tattacgatc tctcgcaggc aggggtgccg
      421 gtacattcga cagcatttcg gcccattgat gatgcttctc tggcgcgtaa tcccttccgc
      481 gtttttacct ccttactccg ccttgagctt atcgagaacg aaattttgcg ccagaaagcg
      541 gcggagattc tgcgtcagcg cgatatcttc accccacgtt gtcgacaact gttagaggaa
      601 tatgagcagc agggcggttt taacgaaaca caggcacagg agtttgtgca ggaagccctg
      661 gaaacgtttc gctggcacca gtcagcaacg gtagatgaag aaacctatcg cgccttgcac
      721 aacgaacatc ggttgattgc tgatgtggtc tgttttcctg gatgccatat caaccacctg
      781 acgccacgta cgctggatat tgaccgggtg cagtcgatga tgcctgaatg cggaattgaa
      841 cccaaaattc tcatcgaggg gccgccgcgc cgcgaggtac cgattttact acgccagacc
      901 agctttaaag cactggaaga gacggtgttg tttgcggggc agaaacaggg cacgcatacc
      961 gcgcgctttg gtgaaattga gcagcgtggc gtggcattaa cgccgaaagg gcgacaactg
     1021 tatgatgatc ttctgcgtaa cgctggaacc gggcaggata atctcactca ccaaatgcat
     1081 ttacaggaaa ccttccgcac ttttcctgac agtgagtttt taatgcgtca gcaagggttg
     1141 gcatggttcc ggtaccgtct gacgccttcg ggtgaggcgc atcgtcaggc gattcatcct
     1201 ggagacgatc cacagccctt aattgaacgt ggttgggtag tggcgcaacc catcacctat
     1261 gaagatttct tgcccgttag cgcggcgggg atcttccagt caaatctggg taatgaaacg
     1321 cagacacgca gtcacggtaa tgccagtcgc gaagcatttg agcaggcgtt gggttgtccg
     1381 gttttggatg agttccagct ttactaggaa gcggaagaac gcagtaaacg tcgctgtggt
     1441 ttgctttaaa ttctgaccat ccgcctttgc aaaaatttgc ctgattttac aaacgaatca
     1501 ggctcatccc atcgacataa aaaaaatgcc gatttatgca tattctctca gttcaacaat
     1561 tggattatta ataaatattg tctagagtga gcggtcataa ataagcactt tcttgccgct
     1621 gaaaacgacc agcgcgggac cattcacaac accagaagga ctcactttca ggtatggatc
     1681 gtagacgatt tattaaaggt tcaatggcta tggccgccgt gtgcggtacc agcggcattg
     1741 cttctctttt ttctcaggcg gcattcgcgg cagattctga tattgccgac gggcaaaccc
     1801 agcgttttga cttctccatt ctacagtcaa tggcgcacga cttagcgcaa acagcgtggc
     1861 gtggtgcgcc tcgtccgtta cctgacacgc tggcgacaat gacgccgcag gcttataaca
     1921 gtattcaata cgacgccgaa aaatcgctct ggcataacgt tgagaaccgt caactggacg
     1981 ctcagttctt ccatatggga atgggattcc gtcgccgcgt tcgtatgttt tctgtagatc
     2041 cagcaacaca tctggcgcgt gaaattcact ttcgcccgga gttgttcaaa tacaacgatg
     2101 caggtgttga taccaaacaa ttagaagggc aaagcgatct cggctttgcc ggttttcgcg
     2161 tgtttaaagc ccccgaactg gcgcgccgtg atgtagtatc atttctcggc gcgagttatt
     2221 tccgcgccgt tgatgataca tatcaatacg gtttgtcggc ccgcggcctg gcgatcgaca
     2281 cttacaccga cagtaaagaa gagttccccg actttaccgc cttctggttt gatacggtaa
     2341 aaccgggggc aactaccttt accgtttatg cgttgctcga tagcgccagt attactggtg
     2401 cctataagtt cactatccat tgtgagaaaa gtcaggtgat tatggatgtg gaaaatcacc
     2461 tgtatgcgcg caaagacatt aaacagctgg gcattgcgcc gatgaccagt atgttcagct
     2521 gcggtactaa tgaacgtcgg atgtgcgaca ccattcatcc gcaaattcat gactctgatc
     2581 gtctgtccat gtggcggggc aacggcgagt ggatttgccg tccgctgaac aatccgcaaa
     2641 aattgcagtt caatgcttac accgacaaca acccgaaagg gtttggttta ttgcaactgg
     2701 atcgtgactt ctcccattat caggacatta tgggctggta taacaaacgc ccaagtttgt
     2761 gggtggaacc gcgtaacaag tggggtaagg gcaccatcgg cctgatggaa atcccaacaa
     2821 cgggcgaaac gctggataac attgtctgct tctggcagcc agaaaaagct gtaaaagcag
     2881 gtgatgagtt tgcattccag tatcgtctgt actggagtgc gcaaccgcct gttcattgcc
     2941 cattagcgcg cgttatggcg acgcgtaccg gcatgggcgg tttcccggaa ggttgggcgc
     3001 caggtgaaca ctatcccgaa aaatgggcgc gtcgttttgc cgtcgatttc gttggtggtg
     3061 atctgaaagc tgccgcgcca aaaggcattg agccggtgat tacgctttcc agtggggaag
     3121 cgaagcaaat cgaaattctc tatattgaac ccatcgatgg ttatcgtatt cagtttgact
     3181 ggtatccgac ttcggactct actgatccgg tcgatatgcg gatgtatcta cgttgtcagg
     3241 gggacgctat cagtgaaaca tggctgtatc agtatttccc gccagcgccg gataaacgtc
     3301 agtatgttga cgaccgcgtg atgagttaat cgttttttct tcggcacctt cttcgggagg
     3361 tgccgtctgg ttaaacacga tcccgctcgc atttttccct aagttaaatg agtaatctga
     3421 tggtgtgtat ttcagataca ccttgtcagc cactaacagg gagtgcgtat gtttccagaa
     3481 taccgagatt taatatcccg tctgaaaaac gaaaatcctc gctttatgtc cttgttcgat
     3541 aaacacaata aacttgatca tgaaattgcc agaaaggaag gttccgacgg tcgagggtac
     3601 aatgcggaag tggtccgcat gaaaaaacaa aagctacagt taaaagatga gatgctcaaa
     3661 atcctgcagc aggagagcgt caaagaggtg taaactttcc taagccgcca gccaggcggc
     3721 ttttttaaca acagcatgga ttgactggag ataagatgac tgaaacgata aaagtaagcg
     3781 aatcacttga attacatgct gttgcggaaa gtcacgtcac acctctttat cagttaatct
     3841 gtaaaaataa aacctggtta cagcagtcgc taaactggcc gcagttcgtt cagagtgaag
     3901 aggacacgcg aaaaacggtg cagggtaatg tgatgttgca tcaacgcggc tatgccaaaa
     3961 tgttcatgat tttcaaagaa gatgaactta tcggcgttat ctcgtttaat cgtattgaac
     4021 cactgaataa aactgctgaa atcggctact ggctggacga atctcatcag gggcagggga
     4081 tcatttctca ggcgctgcag gcattgattc atcattacgc tcagtctggt gaacttagac
     4141 gcttcgtgat caaatgtcgg gtggacaatc cgcaaagcaa ccaggtcgct ttgcgcaatg
     4201 gttttatcct tgaaggttgc ctgaaacagg ctgagttcct gaatgatgcc tatgatgatg
     4261 tgaacttata cgcgcgtatt atcgattcat aataaccctg ccagcggcgt tcgcgtaatg
     4321 cgctcctcgc cgttaatgac ttttggacca cggacatgaa ccgtgtcgcc atcaaaggct
     4381 tcaacgacgg cgtggtcggt taactccaca tggttttcaa ttatcaccgc acctgaaata
     4441 cggctctcac cttgtattac aacgtgctcg tcgagcagaa tcggtccacc acgtaccacg
     4501 gcatttccgc caatcaagac atgatgtttt aacacacaat taccttccac aatggcatat
     4561 tccgccacct gcgaactgta atgaatcgtc ggaatggcat cttcttctat gccagctttc
     4621 acctgcgcgt gaccgtagac tttagcgcaa tcgcatagcc agacgttgtt ctcttcatta
     4681 ccttcaacac tggcaaaatc aaaaacttcg gcgcgatgtt caataaaagc ataccggacg
     4741 acggcatcgc cataaatttg tgcctggtgg acaatacgcg aggcgctaac ccttgcgcga
     4801 tcataaattt ggagcaggag ttgatggtcg ggcgttaagc cttgtgcggc gacgatcata
     4861 gaatgttgat caattaaggc gtgaccaaat attctacatt gcccgtaaac taatgagtca
     4921 cgaatggtga cactgtcact tatatatgca ccctggctga tttcactatt atcgatccag
     4981 acgttatccg ttgcgtaaac ctctccccac agcacgctgg tgccggtgat acgcgtatta
     5041 ccagaaatca ccgttccgcc gaacgcaata gcattctggt cgtaaatcca gcaatttcct
     5101 tcctgtgcta acaccgtttc gcgatcgatc cagccgccag cggttcctgc tatcacatcg
     5161 ttaaaatcgc taatggcgat aatctgccgt aataacacac tttttttagt gccatcttcc
     5221 tgataactaa aggctcgctg ttcttcactt agacgatatt tgcgcatagc gttttctcac
     5281 aggtgactta ctataaccgt agcaaattct gcggctctgg ctatgctcga gaaaattcca
     5341 taaaatgcat ttcaaatata ctttataaat taaacaaaat gagtaagaag atgcagagcg
     5401 ataaagtgct caatttgccg gcaggctact ttggtattgt gttggggacg atagggatgg
     5461 gatttgcctg gcgctatgcc agccaggttt ggcaggtcag ccactggtta ggggatgggc
     5521 tggtgattct ggcgatgatc atctggggat tattaactag cgcatttatt acccgactca
     5581 tacgctttcc gcatagcgtg ctggcggaag ttcgccatcc agtgctgagc agttttgtga
     5641 gtttgtttcc ggcaacgacg atgctggtgg cgattggttt tgttccgtgg tttcgcccac
     5701 tggcggtgtg cctgttcagt tttggtgtcg tggttcagtt ggcttatgcc gcctggcaaa
     5761 ctgcgggatt atggcgcgga tctcaccctg aagaagctac cacgcctgga ctgtatctgc
     5821 cgacagttgc caacaacttt atcagcgcaa tggcctgtgg tgcgttgggc tacaccgacg
     5881 ccggtctggt gtttttaggc gcaggcgttt tctcatggct aagtcttgaa ccggtgatct
     5941 tgcagcgtct gcgcagttcg ggagaattac ccacggcact gcggacatca ctcggcattc
     6001 agctcgctcc tgcgctggtg gcctgtagtg cctggttgag cgtcaacggc ggcgagggtg
     6061 atacgctggc gaaaatgctt tttggttatg gactgctgca actgctgttt atgctacgtc
     6121 tgatgccatg gtatctctcc cagccattta atgcttcatt ctggagtttc tcgttcggcg
     6181 tatctgcact ggcaaccacc ggtttgcatc tggggagtgg cagcgaaaat ggatttttcc
     6241 atacgctggc ggtaccgctg tttatcttta ccaattttat tattgcaata ctgctcatcc
     6301 gtacttttgc gcttctgatg cagggaaaat tgttagtcag aaccgagcgc gccgttttaa
     6361 tgaaagcaga ggacaaagaa tgatcattcg tgacgaaaac tattttactg ataaatatga
     6421 attaacccgc acacactctg aagtactgga agcggtgaaa gtggttaaac cgggtaaaac
     6481 gctggatctg ggctgtggca atggtcgtaa cagtctttac ctggcagcca atggttatga
     6541 tgttgacgca tgggataaaa atgccatgag tatcgccaat gtagagcgca ttaaatccat
     6601 tgaaaatctg gataatttac acacccgagt cgttgatctg aataacctca catttgatag
     6661 acagtacgat tttattcttt cgactgtggt actgatgttc cttgaggcta aaaccatccc
     6721 agggctgatt gccaatatgc aacgttgcac taaacctggt ggttacaacc tgattgtggc
     6781 ggcgatggat accgctgatt atccatgtac cgtcggcttc ccgtttgcct tcaaagaggg
     6841 agaattacgt cgatattacg aaggctggga gagggtgaaa tacaatgaag acgtcggcga
     6901 gctgcaccgc accgacgcca acggtaatcg tattaaactg cgtttcgcca cgatgctggc
     6961 acgtaaaaaa tgacccgcta agaataaggc gcgtgaaaat tccccacgct gagatgattt
     7021 actgttcttc ttttcggtaa gcatattttt tatcgaaggg atgtgaaatt aatcacagta
     7081 gtcgaagttt tttagcagct taacttactg aaatttaact actgatgatt gacttagccc
     7141 ctttttcggc attgactatg tcgtctgaaa aggggctgaa aaatttattt tcaccaacac
     7201 tttttttgcc acaacacgaa gcggcgcttt ttgctataac ttaaaaagta atataatcat
     7261 c
//
LOCUS       DAZRUF010000207         7166 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZRUF010000207 DAZRUF010000000
VERSION     DAZRUF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.208"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(1..4519)
                     /locus_tag="ACHXOL_003432"
                     /pseudo
     CDS             complement(1..4519)
                     /locus_tag="ACHXOL_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4539..5000)
                     /locus_tag="ACHXOL_003433"
     CDS             complement(4539..5000)
                     /locus_tag="ACHXOL_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR6810334.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5028..6930)
                     /locus_tag="ACHXOL_003434"
                     /pseudo
     CDS             complement(5028..6930)
                     /locus_tag="ACHXOL_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
BASE COUNT         1329 a         2193 c         1954 g         1690 t
ORIGIN      
        1 tttatttgac tacgtgataa acgaacccca tccaggaaac ccgtatgccc actaacacga
       61 cgaccatcgc caatatagtc catcaaactg ctgattttag aacttgcagc caatcccagc
      121 ggatcaaccc acccacaagg attcggcaca taccggtaag tctgctcccc gccctgcaac
      181 cccagcggat cactcaccag gtacattccg gtttccggct cgtaataccg gaaccgatta
      241 tagcacagcc cggattccgc atcctgccac tgtcctgcat acagcaggcc ggggtcggct
      301 tccgggtcca gttccccgcg cgggtccggg tagccggtgt ctgcgggcag gctgagtgcc
      361 agcccccaca ggctggtctg ccccggtcgc cagaccgtct ttccttcact gttaaagagc
      421 atcagcgggc ggcccgtcag gtcgctcacc gcatgattcg tccgcgtcac ccactgcggg
      481 gccaccgacg gatgcgcctg ccgtacccgg ctgaactgct ggcttatcag ctcaaagccg
      541 ttaaacacca ggtgccttac gctgtacagt ttatcatcgc ggtattcccg gatttccgca
      601 atactgtcgc cgtcccacag atacgtgaag cggattttct tccggtcgca gcgtttctct
      661 gttcgtctgc cgctggcgtc gtggcggtat tcccacagct caccctgtgc gctgcaatac
      721 ccggtcagct ggtcccggct gtcccaccgg aaccgctctg tttccggtcg gtagccgtca
      781 cggtgtctgg tgcggctgac catccggcct gcggcgtcat agtcatactg cgtgtttccg
      841 gcctgtttca gccggtggcc tccggcatac tggtcagtct cttcacttat ccggtgcctg
      901 cccgcagact gcgccttcag gtagccgcag ctgtcatagc cataggactc atcgccttcg
      961 ccatagccca gcccggtgcc tgaggctgtc accgccgtga cctggccgtt ccggtccagc
     1021 cgatactccc gccgtgtttc ccgtccgtaa tcttcccggc tggtgacgcc tgacacgtta
     1081 cccgcgctgt cgtaacggta ctcccgtacc agtgagggaa ccagcccgtc ccatgtggct
     1141 tctgccggaa aaaatccggc gcgctgactg acaggctgac cggctgcatt ccagccctgt
     1201 tcacaggcaa aaccccggtt actgtgccgg agcacttcac gtcccgcctc atcacggctg
     1261 aacgtcagcg tctcatcccc cggcagaatg acctctgtca gttctccggc ggcgttgtag
     1321 cggtacgtgg ttttcagcag ggattcaccg gggtgtctgt cgcgttcatc ttccggtggc
     1381 aggatacagc ggatgacggt gtgttcatcg gggtagttgt attcaatggc ccgtcggccc
     1441 tgagtggtga cattcacccg cccgcgttcg tcatagccgg tgcgctgtga tgtgccgtcc
     1501 gggctgaaga tacccgtcag cctgccggat ttgtcatagg catactgcgt tttgccttcc
     1561 ggcgagatga tttctgccag gcggcctgcg gcgtcccggc tgtagaggat ggtttcctcc
     1621 aggccgttaa ccctggcggt acagaggcca tcggcgtcat agcggtaacg ccagaccacg
     1681 ccgccccagt cacgctcttc cgtcacccgg ccgcagttat ccagaacata aagatatgac
     1741 tcaccctgcg ggttgatgac ttctgtcagt tgcagcgtgt ctttatcgta gcggtactgc
     1801 cagcggtggc cttcaccgtc cgttcgcgcc accggaaaat cgaacggacc gtattccatc
     1861 gtccagacaa caccgttgcc gtccgtccag gccagcaggt tgccgtggcg gtcatagcga
     1921 aatccttccg cgccggttcc agcacgttca atgccgctca gtttatggtg ctgctgccac
     1981 tgtaaccgca ctgactgacc gccggggccg cgcatcttca ctggcaggtc gtgaccgctg
     2041 tattcggtgt ggtgttcacg gcccatcacg tcggtgagtg tttccggccg gttgtggtgg
     2101 tcgtactcta tgcccagacg ccgttcatcc tggcggaata ttcccgacag cagcccctga
     2161 gcggtgtatg ccagccgggt ctcttcgccc tgtggcgaca ccagacttac cggcaggccg
     2221 cgttcatcac actgctgtgt ccacgtctgc ccggcagggt cggtgatgtt gctgagcagg
     2281 ccgcgctcat catagcggta ctgccactcc gcgccgtctg ccgccgtgta acggcttaac
     2341 tgaccgtgtg cggcgttgta ttcaaatttc tccacccgcc cggcagggga gtggcggggc
     2401 agcaggtgat ggaattcatc ccattcatac atggtggtgc tgccgtccgg cgcttcttcc
     2461 cgcagaatat tgccgtcagg atcatagtaa taacggtgaa tgcccttgcc gtccgtcacg
     2521 gtggtgcagc cgtcaccata gtccagcgtg gcgttgtaat agccctccgc cccggtgaca
     2581 ttcacgcacc ggccctgttc atcgtaggtg aaacggctcc acgtctggtc gttatcggac
     2641 cagcggatga tccggtccgc agcgtcgtac tcataaaaca ggtggtagtc cagccgcgca
     2701 tccgcttctg tcagccgccc ccgcgcatcc tgttcgtagg tcgccagcgt ctgtatgtcg
     2761 ttatccgtgc ggtgaatggc tttcaggtag ccgctggcgt ggtacgtcag tttaagccgg
     2821 atcccgtcgg tgtgactgac acccgtgatc cggtactggc tgtcgcgctc aaacgtgatg
     2881 cggttgctgg cggtgtcgct catcccgccc agcatgtaca tccggtcatt ggggctgtgt
     2941 gccaggcggt catcgctggt atcgtcagtc accgggtggc gcagacgcat tcccggaaag
     3001 gcgtcataaa agaagcggct gctcagtgca tccctgtcga acagctccag ataatcatcg
     3061 cggcggcgca gaatgtactg ggcgtgatac gggttaaccg ccgtatcact gtcaccgtcg
     3121 aaggcaaatc tcagcgtggc tccctggctg atgatatcca cccggtttcc ctgttcacgc
     3181 acccaggcgt attcgctcca ggagtcgctc cagcccacgc cggtcaggcc atgcagcggc
     3241 agtacagaac tgtggaaacg ggtgaaggag agggggaggg tctggcccag ggtgaagtcg
     3301 gtacgttcgt cgcagtatgc gccggttacc ggatcgacag gatctgttac ccatctgatg
     3361 actttattgg caatactttt taacgactta cctgttttcc ccagcaacgc gccgagcttt
     3421 gcgcccagca gcttcccgct tttccccagc agtttcccag tttgccgagt ctcgccagat
     3481 tcttcagttt tcccagtagt cccgctggcg gcagaagata gctggctatc gttattcccc
     3541 ccaggatcat tctctgccac ggcgggaact ccggctggat atccagatat gtgacctgct
     3601 caccgccaat aaagacgtcc ggcgaacctc ccacgatcgc cgcactgcac tccagtttgt
     3661 cgcccttgcg cgcggcaggc tgattgttga taaagacgtt gctgctgccc tgggctatcc
     3721 tcggtggttt gttctccttg tcgcagagcg ccgtgtcctc accggcttcc gggaaatcca
     3781 catctttttt gatttcgcca cgcccgccca ccatggtggc aaactccatt aataccggac
     3841 cggcgggaaa cagaagcgat gcgccggcgc tgacgcgatc cataaaaccg gcattgctgt
     3901 ccgtcagggt gataacagcg ttcccccagg ccttcgccag tggtactgct ttttccgctg
     3961 ccgattcaag caggtcagtg gcaatatcac cccatgaggg ttccccctgt gggacttcag
     4021 gtattgctcc aggtggggga ggagggggaa gggtgaccgc tgcacgtgcg gccagctcgc
     4081 cgttaatttt tacgttttca gaaccggtat tgatggcgcc gtccgtgtca caaaagctgt
     4141 ttatccatga ttcaagctgg ttctgtttct cctccacata atcactcgcc ttatagctgg
     4201 catacatcag cgcaggtacc agaaccagcg cataagggcc ggtggcacag gcggccataa
     4261 tcaggcaacc ttctgcgatg gtcactatcg cccccagtac ggcccctgcc agcgccccca
     4321 aaaaactctt gtgctgaatt tcatcgccca gcctggcggc gggggggcca gacttttttg
     4381 atgccaacat ctgtgcaatg gctttgtcgt tggctgcccg tttcatctgg ctggcgacca
     4441 tcgcctgctg tgacagcatt cgcattgcca gcgtaccgcc tgcggtcgct ccctgtggcc
     4501 cgcctggtcc ttcactcatg tgttatccct cgtttatgtc agcgcggctg gtccttttgg
     4561 atgtcattct ccggtggcaa cggggtgaag ctctttatca cctccagcag catcgtcttt
     4621 tgactgttct tcagggataa ggaggtgctg aaagtgaagg tcagcagcgt atctccacgc
     4681 cgctggaata cggctatctg gctaatccgc ccctgattcc cctcatcttc cggaccgttc
     4741 caggaataat ccagcagcca ggccggggcc tctgccagag tgatttcccg gcaggcatgt
     4801 tcggtgtacc catcaagttt gccctggtac agcgccagcg tttcctggta cagctctggt
     4861 gtcagggttc cctgtggaac ggcttcgcgg gtaatgacca ggttgatgcc actgttatct
     4921 gtggacacca ggacctgcat tgactgatcc tgccattgag acggcagttg aatgtggcct
     4981 tcattaaaag tgaactgcat aaaatactcc ctgataatct gagtgaatca gttgatgaaa
     5041 acagaaccgc ccacgatggt gatgccatcc ttcgacagcc tgattaccgt cgattccccg
     5101 acctgtagct gaatgctatc cggcgtcagc gcaattttgc tttcctccac ggccagcaaa
     5161 atttccttaa tcgccgtata agcctgtata ccgtcgcctt cgccaccatt gtttacagcg
     5221 tcaacatgca ccgaaccggc aatcgtgcct ctgtcctgaa agacatcctc agataaagac
     5281 cctttcagta ctccggtgat cttgtggcct ttttccaccg taagaaggcg gttaccttcc
     5341 tggatcgtga ccgtctggtc attttttacg gtttcgcggt gatcgtgttt tacggtggtg
     5401 gtacggtcgt tgagcacttc ggtatccatg ttcttctgcg cgtggatgta gacctgttct
     5461 ctccctgtcg catcatcaaa cttcagctca ttaaatccgc tgcccatgta cgttttggag
     5521 cggatggtca tctgcgtctt tgttcccggc aggctgccgg ggggggcggt tttcctggtg
     5581 gtaggtgcgc cccataatga tcggctggtc cggatcgccg ttgaggaagt ctacaatcac
     5641 ttcctgaccc acacgcggta tcgccaggtg gccaaaaccg gtgcctgccc acgcctgtgc
     5701 cacacggatc cagcacgaac tgtcttggtc tgccgggtta taacggtccc agttgaactt
     5761 cacccgcacg cgaccatgtt cgtcgcagaa gatttcctcg cctgccggtc ctgtcacgac
     5821 agcgctctgc gggccgtcga ccagcggttt cagcaacggc tgtggtcgcc atgttctgtc
     5881 tgccgggatc accgcaaaat ggttctccag cgccgtcccc gctccctgcc gtcctggcac
     5941 cgcctgtggc tgttcgccgt gcagttcact tgccaccacc tgccattccc ggttcaggtt
     6001 cgcctgcgga tgccccgtca gcacaattcg tcgtcccggc catatctccg gcgagcgact
     6061 cattccccgc gcggtttctg cattgtttcg ccagccgtcc atctgccagc gggcaaagtt
     6121 ctgcccgtga gcacccttga aacgtccggg gtagtcatac acttcatact gcgtgcgctg
     6181 gtagtcctgg tgctggcctt cctgttcaaa acgtccggcc cagccggggc gtttaaaggt
     6241 gtagtctttg gtcaccacgg aagaagggcg gatttgtgcg ctgtagcgga actggctgat
     6301 gcagagggtg ctcacctcgg tacgggtgtt cgggttccat gggatttcaa aagattcggg
     6361 cagatggcgg actgtgtcgc acagcaccag gctctggtcg gtacttttgt aagcatgctc
     6421 ctcataaaag aagatgcctt cctccgccgc catccggcac aggaaatcgt aatcagtctc
     6481 accgtactgg acacaaaact cacgggaagg atgcggctcg ctgaacagcg gactccattc
     6541 ggtcaccccg ttttcctgca acatcgtgcc gaggatgctt ttgatgtcct cgttctggaa
     6601 gatacggaag ttctggcgca gaccggcacg ccacagcggc gggtgcacct tcatgctgta
     6661 cagcatctgg tttttgtcgt tctccccgag ttcaaaccag gtcaccacgc ctttcacccg
     6721 gcgctgtact tcatcgccct gccatatcgt caggtaggcc attttgtcca gcacctgcgc
     6781 aaattcaagg gagagaaact gctggctgac cagggagaga tcgagggaaa agagcgaaga
     6841 gagtgactgg ttcagatgaa aggaaaccac cgcaaaagcg tccggcggca ggccgtccac
     6901 ttccagtgtg aaacgtaatc cggttgacat agcaacctcc agtaaaatga ataatgatcc
     6961 gtaccgtaca actgcggata atgtgataag cgggacgact tcagacgtta ttagggaagg
     7021 aacagaaaat gctatggtta tgtgagaatt ttgtttaaat attactttac agaaacgggt
     7081 gtctgttcag tcaaaaaaat aatcacaagc tcaacctctt gtttcttggc gttattcagg
     7141 acaggtacgc atatcacaac gagtaa
//
LOCUS       DAZRUF010000208         7122 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZRUF010000208 DAZRUF010000000
VERSION     DAZRUF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.209"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..436
                     /locus_tag="ACHXOL_003435"
     CDS             <1..436
                     /locus_tag="ACHXOL_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR6810335.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            519..1421
                     /locus_tag="ACHXOL_003436"
     CDS             519..1421
                     /locus_tag="ACHXOL_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR6810336.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="ACHXOL_003437"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="ACHXOL_003437"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR6810337.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="ACHXOL_003438"
     CDS             complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="ACHXOL_003438"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR6810338.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="ACHXOL_003439"
     CDS             complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="ACHXOL_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR6810339.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENV
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5331..6038)
                     /locus_tag="ACHXOL_003440"
     CDS             complement(5331..6038)
                     /locus_tag="ACHXOL_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR6810340.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="ACHXOL_003441"
     CDS             complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="ACHXOL_003441"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR6810341.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6896..7099)
                     /locus_tag="ACHXOL_003442"
     CDS             complement(6896..7099)
                     /locus_tag="ACHXOL_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR6810342.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1639 a         1989 c         1856 g         1638 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcgacat cgttcgtctg gatgccttca aaaccagtaa
      121 agagattcag tttgcggtgc agtcagggcc aatgttgatg gaaaacagtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt gatggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagaggaaa ggttaagcat tgtacactcg aggaggccag acaaactggg gctggctaca
      481 cagctgaaaa atcatcagat aaatgaaggg aaacaaaaat gaaaaaaacg ctcgcaacgt
      541 tatttttatt aacgtgtctg ggatcatcat ccgcttatgc ggataacgct ttgattttac
      601 aaactgattt tagcctgaaa gatggtgccg tctcggcgat gaaaggggtc gcattcggtg
      661 ttgaccataa tcttaaaata tttgatttaa cacacgaaat tccaccgtat aacatctggg
      721 aaggcgctta ccgcctgtat cagaccgcca gttactggcc gcaaggttcg gtatttgtca
      781 gtgtggtcga tcctggtgtt ggtacagacc gtaaatcggt agtgctaaaa accaaaaacg
      841 gtcaatactt cgtttcacca gataacggta cgctcacttt ggtggcggaa tcgctgggta
      901 ttgagagcgt ccggcaaatc gatgaaaaaa ccaaccgcct gaaaggttcc gaaaagtcat
      961 ataccttcca cggtcgtgac gtgtatgcct acaccggtgc acgcctggct tccggagcga
     1021 tcacgttcga acaggtgggg ccagagcttc ctgcaaaagt tgtcgaactt tcttatcaga
     1081 aagccaaagc aacaaaaggt gaagtgaagg gcaatatccc gattctcgat attcagtacg
     1141 gcaacgtctg gagcaatatc agcgacgagt tgctgaatca ggcaggaatc aaactcaacg
     1201 acacgttgtg tgtaaccatt tccgaaggtt cccagcaaaa atacgcaggg aaaatgccgt
     1261 atgtcgcaag cttcggcgat gtgccggaag gccagccgat ggtgtatcta aacagcttat
     1321 tgaatgtttc cgtggcgctc aatatggata atttcgcgca gaaacatcaa gttgcgtctg
     1381 gtgctgactg gaatattgat gtgaagaagt gcgctaagta aggtgctgat gcaggatgcg
     1441 gtgtggcgca ccgcatcctg caacggagtt gtaaattaca ccgtctccag ccgggcgtaa
     1501 gccgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg gctatgttca
     1561 ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg cacgcgctgg
     1621 ccgagtttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg atggctaacc
     1681 gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg cagctcgccg
     1741 ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt ttccgcgtag
     1801 gtcagcgtca gtttctgcat cgcacgggtt acgccaacgt aggccagacg gcgttcttct
     1861 tccagacgtc cgccttcatc cagcgacatc tggcttggga acatgccctc ttccataccg
     1921 acgataaaca cctgcgggaa ctccagccct ttcgccgagt gtagcgtcat caactgcacc
     1981 gcatcctgcc aagtatccgc ctgcccttca cctgcttcca gtgccgcatg ggagaggaac
     2041 gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg cgttgccgtc
     2101 accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc ctgctcgtac
     2161 atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag cggcatatcg
     2221 gcagtttctt gcgctaaggc atcgattaat tccataaatc gctgcaaggc gctggcagct
     2281 cgcccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag tgttaactgg
     2341 cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc ccgcgttggc
     2401 gtattcacca cgcgctcaaa cgccgcgtcg tcgttacggt tggcaatcag acgcagatac
     2461 gagagcgcgt ctttgatttc ctggcgttcg aagaagcgca tcccgccgta aatacggtac
     2521 ggcatactgg cctgcaacag tgcttcttcc agtacacgcg actgggcgtt actgcggtag
     2581 agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat gcggttaacc
     2641 acaaaacgcg cttcgtcgag ttcgttaaaa gcgcaataga gggaaatagg ctcaccgtcc
     2701 gcgccatcgg tccacagttt tttacccaga cgcccgttgt tgttttcaat cagggcgttc
     2761 gcggcgctca gaatattgct ggtagagcgg tagttctgct ccagacggat agtttcagca
     2821 ccggggaaat cattgaggaa acgctgaata ttctctacct gcgccccgcg ccagccgtag
     2881 attgactggt cgtcatcgcc aacgatcatc actttgccgg tatcgcccgc cagcaggcgg
     2941 atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat gttggtaaaa
     3001 cgttcgcggt agtgttgcag gatatgcggc ttgttaagcc acaactcgtg agcgcgcagc
     3061 agcagctcgg cgaagtccac caggcccgca cgatcgcacg cttcctgata cgcctgatac
     3121 accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg cggacgcagg
     3181 ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca ctgcttctcg
     3241 tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc actgtcgagg
     3301 atctggaaat cctgcggtag attggcgtcc atatggtgcg cacgcagcaa acggtgcgcc
     3361 agcccgtgga aggtgccgac ccacatgccg ccctggctgg tgcccatcag ttgcccgata
     3421 cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat cgagtatggc
     3481 gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg cgtcttacca
     3541 ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc ttcgcgctgt
     3601 ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc gtaaaaatag
     3661 gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac agatttactg
     3721 attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgcggc agtaaacggc
     3781 tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc tgcattccgc
     3841 tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag atctcgccga
     3901 tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt tttgagcgcc
     3961 cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac agttccggct
     4021 gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt tttaaggtgt
     4081 cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta atcattgctg
     4141 cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc gcttgttcaa
     4201 tcgaacgaaa acgccaacgc gtcacgtcgt gataaatctc gggttccgct tcccgtaccg
     4261 cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga tgataatttt
     4321 gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta tcgtaaaggg
     4381 tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa aaacgcatta
     4441 tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt gttgaaaatc
     4501 aagatgagta tagatttgcg tggtggagag gttggcatga cccagcagct cctgcacacc
     4561 acgaagatcg ccgctcgact ccagcatatg cgtggcgaac gagtgacgta atttatgcgg
     4621 atgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt tctgcacatt
     4681 acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt cgctaccaaa
     4741 caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac caatcggcag
     4801 gcggcgctct ttgctgcctt tccccattac ccacacttca ccagactcca ggtcgaggtg
     4861 tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca tcacttccag
     4921 cattgcacgg tcgcgtacag cgaggggatc gttgatatca atgtccagca gccgattcat
     4981 atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg aaacaccttt
     5041 cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc tacgtagcgc
     5101 agaaagccgt aacgcgaggc ttgctgctcc cagcccttta cggcgactgc gtacagcaaa
     5161 attgcgcacc atcgtcacat cacattgctg ccagctttgc aggacgtttt cgctggcaaa
     5221 attgatgatc gcctcaagct gacgctggta gttaagcagg gttatcgggc taagctggcg
     5281 ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg tcatacgcgt
     5341 tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag taactgcgtt
     5401 ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacgac acccaaatca
     5461 gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc cggtagcacc
     5521 accagcagct ctggtccgtt aagcggccca agatagtgct gttcctgccc caaacgctga
     5581 atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt cgacggcgca
     5641 cctaagcgcc agcgatccgg aaacaggcgc agactcgcac ctgccaggcc gagatcgcgc
     5701 gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt gagactgcgc
     5761 tgcaggtaga gtaggcgata aaacaggcct tcgttggcga tagcctgttc catcaacagc
     5821 gccatgttct cttccagaac atgaatatga ttacgtgcgc gggccatgtg ccactcgacc
     5881 aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc gcgcgcatta
     5941 cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc atcaagctcc
     6001 gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat ccgtcgtaga
     6061 catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag gcgatatcaa
     6121 gacgaccgcc ggggagttcc acgcgtactt cttcggccag caaaccttgc tgaatcccta
     6181 ctgcaaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc ccacgctcat
     6241 aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata ttggcgcgct
     6301 ccggaaaacg ctcgtggctt tccagaacag gaccaagcgt ttctaccgcc gcggtatcga
     6361 catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg cataagattg
     6421 tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga aacggcacgg
     6481 cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca tcggtgacgg
     6541 tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg gtcagtcctt
     6601 tcagacgcac aaaacgggca aagcagcgcg caccgttgcc gcactgcgcc acttcactgc
     6661 catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc ggctcaacca
     6721 ccagcagttg gtcaaaccct acccccaggt gtcgatcagc caggcgacga atcagctccg
     6781 gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg ccaaggccat
     6841 gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact gacttttagt
     6901 aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc accgtggatt
     6961 gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca ggcgggaaat
     7021 agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat acagtgagtg
     7081 ccttaaacac gtttttcatt atggattgcc tgtaaagttc at
//
LOCUS       DAZRUF010000209         7106 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZRUF010000209 DAZRUF010000000
VERSION     DAZRUF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.210"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(104..418)
                     /locus_tag="ACHXOL_003443"
     CDS             complement(104..418)
                     /locus_tag="ACHXOL_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR6810343.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(423..638)
                     /locus_tag="ACHXOL_003444"
     CDS             complement(423..638)
                     /locus_tag="ACHXOL_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR6810344.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(789..2642)
                     /locus_tag="ACHXOL_003445"
     CDS             complement(789..2642)
                     /locus_tag="ACHXOL_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR6810345.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCCGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            3397..3513
                     /locus_tag="ACHXOL_003446"
                     /pseudo
     CDS             3397..3513
                     /locus_tag="ACHXOL_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3618..3694)
                     /locus_tag="ACHXOL_003447"
     tRNA            complement(3618..3694)
                     /locus_tag="ACHXOL_003447"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3658..3660),aa:Arg,seq:tct)
     gene            complement(3708..3784)
                     /locus_tag="ACHXOL_003448"
     tRNA            complement(3708..3784)
                     /locus_tag="ACHXOL_003448"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3748..3750),aa:Arg,seq:tcg)
     gene            complement(3792..3867)
                     /locus_tag="ACHXOL_003449"
     tRNA            complement(3792..3867)
                     /locus_tag="ACHXOL_003449"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3832..3834),aa:Ile,seq:cat)
     gene            complement(3908..4966)
                     /locus_tag="ACHXOL_003450"
     CDS             complement(3908..4966)
                     /locus_tag="ACHXOL_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR6810346.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(5074..5314)
                     /locus_tag="ACHXOL_003451"
                     /pseudo
     CDS             complement(5074..5314)
                     /locus_tag="ACHXOL_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5539..6360)
                     /locus_tag="ACHXOL_003452"
     CDS             complement(5539..6360)
                     /locus_tag="ACHXOL_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR6810347.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(6357..6731)
                     /locus_tag="ACHXOL_003453"
     CDS             complement(6357..6731)
                     /locus_tag="ACHXOL_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR6810348.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            complement(6731..6988)
                     /locus_tag="ACHXOL_003454"
     CDS             complement(6731..6988)
                     /locus_tag="ACHXOL_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810349.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            complement(6985..>7106)
                     /locus_tag="ACHXOL_003455"
     CDS             complement(6985..>7106)
                     /locus_tag="ACHXOL_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810350.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
BASE COUNT         1672 a         2011 c         1634 g         1789 t
ORIGIN      
        1 cagcgccaga acagcagctg acaggccgta tttgattttt gcgctcatgg atatttatca
       61 ggatgctacc aatgaaagat actggaaagc caactgcaaa aagctaacaa cccgtaatcg
      121 agttatcaga actgttaatt tttatggtat accgcgcctc tgaacagggg cgcgtttctg
      181 gcaacagctc gtccccttca cataacccgg cagcaacatc caagaagacc tgtctgatgc
      241 tccttctggc tgctgcctca taaaactcca gcgcggcacc ttcaacacgg tccagcgaga
      301 tgtccaggtc aaaaatttca ccgtcaaagc gttttttgtc ccgtaacgct aaagttaccg
      361 taactttatt ctcaaaattg cggatccctt tcacaatcag ttcatagttt tgagtcattg
      421 aattactctc cccgtgcagc cttacgacgg tcctctctga ttttgaaata caggttagtc
      481 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
      541 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      601 tatgacacac ctgttgtgat tttttccatc tggtacatac cccgtctccc gttatccgga
      661 agctgacaac aataaaaaaa gccaccagtt aagtactgat ggctctgata actcatgcag
      721 gcatctcaga cgacccactg acactaccgg tgagtttaac gataccttcc atttggctgg
      781 ctcacttttt atgatgatgc cggtgcattt atctccagca ccagactttc tatctcaacg
      841 ccatacgctg catttttggt aatatccgtc agcgtcagcg cattcagccc cagtgtcaga
      901 ctgtctttta tgacctggaa tgccgggcca gccactccat tcagtttcgg agtaaccgtg
      961 gcactgccgg cggtgaacac cagctccagc gtcagccagt cgttactgta attcccgaac
     1021 tcgcccaact ttgtgtttcc tgctttcttg tgatgcatca gattcagttt gccgtctgtg
     1081 gtctgggtga agaacgacat caggaacggg ttaccagtcc cggtcatcgc cacgacgtca
     1141 ggtaacgcta catcggtata cagataaatt cccagaccga actggttgtt ggtcagtgcg
     1201 cctgacagtc gaaacttaca gctcagtctg ccaccccgtg tcagcaggga gactgcgtca
     1261 tccaccgggc gcgtcaggga ccaggcttta ttgctctgct tggtgatctt aaatacacca
     1321 tctgacaact gaactccgcc attcttaatg ctccagccct gcgcagcagc gtctccggct
     1381 gtcggcaaca gggagattgt gcgtatggat gcatcttcag acggccccga tggcgtgtcg
     1441 ccgccgggcg agggtttgat ttccggtgcc ttaccactaa tgaaggctaa ggtgcgaccg
     1501 gctacgttca gaatagcagt tgccatacga tcgggaataa tgccacgacg cgcccatgag
     1561 ctgaaatgcg tcgggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
     1621 taataaccag acccggcaat atcaggatct tctgacgggt tgtttgtcgg tgtattaact
     1681 ccgctaccat cggtcataaa gggaacaaaa taaatctgct gggattcttt acctttatat
     1741 gcaccatata ccacttcata ttgcgtaccg tgttcttgtt tccacgcgta tgtcgtgtcg
     1801 ccacaaatcc aggggactga tgccggactt ccaccgtgac actgcgccgc cagcccggca
     1861 aggtcagcac ggaactgctg taccattgca agaaatgctg ctggctgctg ggcgtaactg
     1921 gcattcgtca tatcgaattc cccctgcatc cagcatatcg ccagcaaaac gtttttcggg
     1981 tttttctgca atgctgcctt cgtgcggaaa agcagatcct gatataacgg cttacccact
     2041 ccccagcgag ccgaatcctg actggccccc gtggactcgc tgaatgtccc ctccgtgccc
     2101 tgggtgaatg ccgaaccacc acaacagcat ggtaccagca ggatccccgc attattaggg
     2161 atatacggaa gcagtttttt ggcaatatgt aagccctgtc cgacacagcc gtactgccct
     2221 ttgctcaggt cagcccgggg atggttaatc gtactcatat cctgaacatc atgcagacaa
     2281 tggtcagcag gaatgatgtc gttaaatacg catacttcac caccgggagt cactgtgtta
     2341 cgacgggcca gttgcttaat gcgcggatgg ggcgcatcgt atgaatccgg aagcggaaga
     2401 ccttcaccgt aagccatggc attggattgc ccggccagta cgatgacgta gtaccactcc
     2461 ggctcagttg caccactgac gaccacatca ccttctgctg caatcgcctg catcagggta
     2521 taaggggtta tggccaccgg actaccaaac ggctgccagc cctccttcag tttttgtgtc
     2581 agtcgtttcg caaggtctga cggcgatgcc gccctgacca cgtcatagtg tttaaatgcc
     2641 atgaatcctc ccggccggga taatattgtg agtaaaataa ggagcgggct gaagtccgga
     2701 agttacagga caatggcaga agagagacga cagcccgcaa ttcgaaaaag accgcgcagt
     2761 tgcgcagagt gattactatg gggtattatt cgccagctga aatattactt cacgttttat
     2821 tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt ttttgcccac aagaaaaccc
     2881 ctccggagag gggctaaagc cgcgtatctg tatcatcatg cacatggtgc cgggtgcctc
     2941 ccggtgagtt cagcccggtg ccactaaacc cgcgtcattc tcgttttgat aatcagagat
     3001 tataccgtca ccagtcgccc ctccgctcag ggggattcac catgcgaaat ttttttaaca
     3061 aatgcccagt ctgacaggca actgtcaact tactgaattg tgagcaacat agcatttaac
     3121 ggggaacctg ttttctgcag taaaaaggcc caccggagcg gatgggcctg gaaggatagc
     3181 ggtcatgtga tgccggtttc ccggtaactc agcaccggta tctgagtcaa cgttttctct
     3241 actgggtcat ttccgatacg ccctgcctgc tgacaggctt tcatcacatc tgaaaatata
     3301 gcaccctgac tgatactgta gtacctaagg ttccagaaac tgtgatgtat ccggcacaga
     3361 aaagcccctc cggagagggc tggagagtgg cgctatgtgc cattgcatga tgccgggtgc
     3421 ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg
     3481 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
     3541 acagacaact gtcaaccgtc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
     3601 gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga
     3661 aggcaattgc tctgtccggc tgagctaaca acgcatgatg cagataatgg accgccatcg
     3721 gggacttgaa ccccgcgcag ccagcttcga aggctggcgc tctttcctga tgagctaatg
     3781 gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     3841 tcgctctcac cactgagcta aagggccggg agccgcataa taacgacgcg taattaattc
     3901 ttcaatatca tccgttctgg ctgactaaat cctgtacttc ccgaaccgtc tgctcaaaac
     3961 gttcagtctc cagctcaacg ccaattgcac gacgcccgag cgccagtgcc gctttcactg
     4021 tcgaacccaa ccccatgaaa aaatctgcaa tcaggtcacc cggacgactg ctagcgctga
     4081 ttatctgctg cagcatttct gccggttttt cgcacggatg tttcccggga tagaactgca
     4141 ccggtttatg cgtccacaca tcggtatacg gcacctgcac cgtcacgcca aaataccgcc
     4201 gcagatgttt atattcactc agcagctccg catactgccg gttcagtgag gtatacgtat
     4261 ccaccagctg gtggtggagc ttttccagtt caccacgccg atgcttctct tctgccaccc
     4321 gggcaaacag cgcctgtaat ttcagataat cgctttcatt cggcaactgc cactgaccgg
     4381 cactgaacca gtgcgacacc atgtttttct ttcctgtggc atctgcaatc tgttttgccg
     4441 ttatccccag ggcagctcgc gcatcacgaa agtaagaaat cagcggggcc atcacatgct
     4501 gtttcagtgc actgcccttc gccgcatacc cggcatcttt cggacgatac ggcccctgat
     4561 aatgttccgc gaacagaatg cgctctgtgg cggggaaata cgcccgcagg ctttccttgt
     4621 tgcatccgtt ccagcgtccg gacggcttcg cccagataat atggttcagc acactgaagc
     4681 gttcacgcat catgatttcg atatcagatg ccaggcgatg accacagaac aggtaaagac
     4741 ttccggcagg tttcagcacc cgccagaact gcgccagaca ctggtccagc cacttcaggt
     4801 aatcatcgtc gcccttccac tggttatccc agccctcagg cttcacttta aagtacggcg
     4861 ggtccgtgac tatcaggtca acagaatttt cgggtaacga ccggataaat tccaggcagt
     4921 cggcgttgat taactcacaa ctggatattt ttacagtatt aagcatggat cattaagcct
     4981 gtctctgata ggctcattct gcttttgcgc aaagcagtgg gcctgaggtt tgcttgtgaa
     5041 cccaacgcat gagcagatgg ctggtgggtg cccctaacac ccaccagccg cccatttacc
     5101 acaaataaaa aagccttcac tgcggaaggc gtctgtaaca accgaactga tagtctgcca
     5161 gacccgccat aaccagctgg gtcagtatta actggcagcg ttcgcgtgaa aggtaagtat
     5221 tctgcgctat ctccccgact gtcgccggtt cggtaacgct taattcatta aacaccactc
     5281 tggcggtttc tgtcatatcc tgctgtttta gcatgtcttt tccttttccg gttaacgtga
     5341 cacaccaata actcttgtcg aaaaagccag caagctgaaa gacaggtatt caccgccacc
     5401 agcgcgttta ctgtactgac gcgatttcag tcataaaaaa cccgctcggc ggcgggttgt
     5461 agaaactctt ctaacgtcag gcataaaacg cccatcgtta tgacgaattt accacagatt
     5521 ccggaaaaat caaccttgtt acctagttac cttttttaac tgccgctcag cccatgcttc
     5581 ttcaatatca aaccgggtca ccagcgcatc ataaaatttc ttaactgttt tttcccatga
     5641 cgcgcgtgtt atctggtttg tcacctcgca tatagcatta aatgcctccg ttgatggtag
     5701 tctttcatag ccacgaccac cacaacgctg gcagtctctg ataacaggca taccacgttt
     5761 taccgactct tcacgatgaa tggcgacacc acgcccacgg caatccttac aggcggtgga
     5821 aacctcaccc tttccgccac actccggaca ggcaactttt accacctccc tgactttttt
     5881 ccattcttcc cagtaagacg gatacacacc tttcgtacac tttgcccata ccggcggctt
     5941 accatccgga tactggacct tgtttgtaaa aactacgctt tcaataaatt tttccccata
     6001 gcaacaaggg cactgctttt tactcgctgc gctgcgggca taatcctcaa aagcgtacga
     6061 agccataatg cgcatcacta ccggttttat ttctgccgga agttttctca acgccgccac
     6121 acgatcgcac cgactgagtg cataatctgc cagtaattct gttgcccgcg ccctgtcatt
     6181 catactgatg cccattttcc ccaggaacgc agaaaacccc atctcagccc gattctgtgt
     6241 catgccctgc gcggccatca catcagtgat actcagcgca tcttttgacg ttgaggccga
     6301 tgcatcggtc aggcaagggg attttgggga gtagtatttc ggtaaatctt ccagtttcat
     6361 tttttgacct gctcttcatg cattatgggg taaatcttca cccccagacg tccaccagat
     6421 actggctgac cacgaacgat attgatttca tcaaactgct catcgtccat taacactccc
     6481 gcatgtgtca gtgcatccag tggtgctttc aggatattgt ccaggtcacg acggcgctta
     6541 tccggtggct ctgcaataat ctttatcgcc agtcttccgg acaggcttaa tttcagtcgc
     6601 tgctgacgga caataagcgc cacatcccgg cgataacgct caccggcttt tgatacaaaa
     6661 tatgtgttgt cacgacgtcg ccagtaagta ttcaccgtcg gcggataagg taaaataaac
     6721 tcatggcgca tcagcgcagc acctcctgca ccagtttttc aaactttccg actctggttt
     6781 ccagctctgc cacacagtcc accagctcat ctactgcttt ctgtgcgcga tgcttcgcct
     6841 gcatcagttc ccgaagcgcg ggtaccatat ctttacggat agcgtctttt gttatgcccg
     6901 ttttttcgag ttgttcagca tgacgcagca tttcctgcgc gtgtttacgc aattgttcag
     6961 gggtaaaagt cattgtctgg ttgttcaaaa gaaacgctcc atcttactgc tgtcagttcg
     7021 tttattactg tatctgcgcg gattgccggg cttcatggga gtggaaagca cccgtgcact
     7081 ttcctggtcc acaggcagaa aatgtc
//
LOCUS       DAZRUF010000210         7102 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZRUF010000210 DAZRUF010000000
VERSION     DAZRUF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.211"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..269
                     /gene="rutC"
                     /locus_tag="ACHXOL_003456"
     CDS             <1..269
                     /gene="rutC"
                     /locus_tag="ACHXOL_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR6810351.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
     gene            277..1077
                     /gene="rutD"
                     /locus_tag="ACHXOL_003457"
     CDS             277..1077
                     /gene="rutD"
                     /locus_tag="ACHXOL_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR6810352.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPC
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            1087..1677
                     /locus_tag="ACHXOL_003458"
     CDS             1087..1677
                     /locus_tag="ACHXOL_003458"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR6810353.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            1688..2182
                     /gene="rutF"
                     /locus_tag="ACHXOL_003459"
     CDS             1688..2182
                     /gene="rutF"
                     /locus_tag="ACHXOL_003459"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR6810354.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            2203..3531
                     /gene="rutG"
                     /locus_tag="ACHXOL_003460"
     CDS             2203..3531
                     /gene="rutG"
                     /locus_tag="ACHXOL_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR6810355.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3789..3962)
                     /locus_tag="ACHXOL_003461"
     CDS             complement(3789..3962)
                     /locus_tag="ACHXOL_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR6810356.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            4335..4931
                     /gene="wrbA"
                     /locus_tag="ACHXOL_003462"
     CDS             4335..4931
                     /gene="wrbA"
                     /locus_tag="ACHXOL_003462"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR6810357.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            4952..5179
                     /locus_tag="ACHXOL_003463"
     CDS             4952..5179
                     /locus_tag="ACHXOL_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR6810358.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(5217..6458)
                     /gene="agp"
                     /locus_tag="ACHXOL_003464"
     CDS             complement(5217..6458)
                     /gene="agp"
                     /locus_tag="ACHXOL_003464"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR6810359.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(6752..>7102)
                     /locus_tag="ACHXOL_003465"
     CDS             complement(6752..>7102)
                     /locus_tag="ACHXOL_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810360.1"
                     /translation="LNDKKCILHLPEFIFNDNKARLVLDTDPEQKVNEVKNFLTALSD
                     SICSLFIVYSNVASISLGNESFSTDDDLEYGYLINTGNHYDVYLPPELFAQAYELNNK
                     ERNAQIDFLTRYAI"
BASE COUNT         1607 a         1799 c         1892 g         1804 t
ORIGIN      
        1 ataacgtgct gtttgccgat gacccaaagg cgcaaacccg ccacgttctg gaaactatcc
       61 gcaaggtgat cgagacggcg ggtggcacga tggcggatgt gaccttcaac agcatcttta
      121 ttaccgactg gaaaaattac gccgcgatta acgaaatcta cgccgagttt tttccgggtg
      181 ataaaccggc gcgattctgc attcagtgcg gactggtaaa acctgacgcg ctggtggaaa
      241 tcgccacaat tgcgcatatc gccaagtgag gccgcgatga aactttcact ctcacctccc
      301 ccttatgctg atgcgcccgt agtggtgttg atttcgggtc ttggcggtag cggcagttac
      361 tggttaccgc aactggcggt gctggagcag gagtatcagg tagtctgtta cgaccagcgc
      421 ggcaccggca ataatcccga cacgctggca gaagattaca gtatcgccca gatggcagcg
      481 gaactgcatc aggcgctggt agccgcaggg attgagcatt acgcggtggt cggccatgcg
      541 ctcggtgcgc tggtgggaat gcagctggcg ctggattatc ccgcgtcggt aactgtgctg
      601 atcagcgtta acggctggct acgaataaac gcccatacgc gccgctgttt tcaggttcgc
      661 gaacgattac tgtatagcgg cggcgcgcag gcatgggtgg aagcgcagcc gttgttcctc
      721 tatcccgccg actggatggc ggcccgcgca ccttgccttg aggcagaaga cgcgctggca
      781 ctggcgcatt ttcagggcaa aaataattta ctgcgtcgac ttaacgccct caaacgcgct
      841 gactttagtc accatgcgga tcgcatccgc tgcccggtgc aaatcatctg cgccagtgat
      901 gatctgctgg tgccatcagc atgttccagt gagcttcatg ccgccctgcc cgatagccag
      961 aaaatggtga tgcgctatgg cggacacgcc tgcaacgtga ccgaccccga aacgtttaat
     1021 gctctgttac tcaacgggct tgccagcctg ttacatcacc gtgaagccgc cctgtaagga
     1081 attgctatga acgaagccgt tagcccaggt gcgcttagca ccctgttcac cgatgcccgc
     1141 actcacaacg gctggcggga gacacccgtc agcgatgaga cgttacggga gctttatgcc
     1201 ccgataaaat gggggccgac atcagctaac tgttctccgg cacggatcgt gtttatccgc
     1261 actgtagaag gaaaagaacg tctgcacccg gcgctttcca gcggcaatct acaaaaaacc
     1321 ctgaccgcgc ccgtcaccgc tatcgttgcc tgggacagtg aattttatga acggttacca
     1381 ctactgtttc cccacggtga tgcccgcagt tggtttacct ccagcccaca acttgccgaa
     1441 gaaacagcgt ttcgcaacag ctccatgcag gcggcctatc tgatcgtcgc ttgccgtgcg
     1501 ctgggactgg ataccggccc gatgtcgggt tttgaccgtc aacacgttga cgacgccttt
     1561 tttgcgggca gcacgctgaa gagcaatctg ctgattaata tcggctatgg cgatagcagc
     1621 aaactttttg cgcgcctgcc acgtctgtcc tttgaagaag cctgcgggct gttgtaagga
     1681 gcaaactatg aacatagttg atcaacaaac ctttcgcgat gcgatgtcct gtatgggcgc
     1741 ggcggtcaat atcatcacca cggacggtcc atccgggcgc gccgggttca ccgccagcgc
     1801 cgtctgcagc gtgaccgaca cgccgcccac attactggtg tgcctgaatc gtggggcgtc
     1861 cgtctggccg gtattcaatg aaaatcgaac gctgtgtgta aatacactga gcgccgggca
     1921 ggagccgctt tcaaatcttt ttggcggcaa aacgcccatg gaacaccgct ttgccgccgc
     1981 ccgctggcag acgggtgtga ccggatgccc gcaactggaa gaggctctgg tttcgtttga
     2041 ctgccgcatc agccaggtgg tgagcgtcgg cacccacgac attctgtttt gcgccatcga
     2101 agcgattcat cgtcacacca caccctacgg gctggtgtgg ttcgatcgca gttatcacgc
     2161 gctgatgcgc cctgcttgtt aacctcctta aggagacagc tgatggcaat gttcggtttt
     2221 cctcactggc agttaaaatc gacctctaca gagagcggcg tggtcgcgcc ggatgaacga
     2281 ttgccgtttg ctcagacggc agtaatgggc gttcaacacg cggtggcgat gtttggcgcg
     2341 acggtgttaa tgccgattct gatggggctg gatcccaatc tttccatttt aatgtcgggg
     2401 atcggcacgt tactgttttt ctttattacc ggcgggcgcg tccccagtta tctcggctcc
     2461 agcgcggctt ttgtcggcgt ggtgattgcc gcgacgggct ttaacggtca gggcattaac
     2521 ccgaatatca gcatcgcact gggcgggatt atcgcctgtg ggctggttta taccgtcatt
     2581 ggcctggtgg tgatgaaaat tggtacacgc tggattgaac gactaatgcc gccggtggtg
     2641 acgggtgcgg tggtgatggc gattggcctg aacttagcgc ctatcgcagt gaaaagtgta
     2701 tcggcttcgg cctttgatag ctggatggcg gtaatgacgg tgctgtgtat tgggctggtg
     2761 gcggtgttta cccgtggcat gatccagcgg ttattgattc tcgtaggttt aattgtcgcc
     2821 tgtctgctgt atggcgtgat gaccaacgtt ttagggctgg gaaaagccgt cgattttact
     2881 ctcgtcagcc atgctgcctg gttcggtttg ccacattttt ccactcctgc ttttaatagt
     2941 caggcaatga tgctgattgc gcccgtggcg gtgattctgg tggcggaaaa cttaggtcat
     3001 ctcaaggcgg tcgccgggat gaccggacgc aatatggacc cgtatatggg gcgagcattc
     3061 gtcggtgatg gtctggcaac gatgctttcc ggctctgtcg gcggtagcgg cgtcaccacc
     3121 tatgctgaaa atatcggtgt gatggccgtg acaaaagtct actcgacgct ggtgtttgtt
     3181 gctgctgccg tcatcgccat gctgttgggc ttttcgccga aatttggcgc gctgatccat
     3241 accattccgg ccgcggtgat tggtggtgca tcaattgttg tgttcgggct gattgccgtc
     3301 gcaggcgcaa ggatctgggt acaaaaccgt gtcgatttaa gccagaacgg taatttgatt
     3361 atggtcgcag taaccttagt gctgggcgcg ggtgattttg ctctcacgct gggcggtttt
     3421 acgttgggag ggatcggtac agccaccttt ggcgcgattt tactcaatgc gttgttaagt
     3481 agaaggttgg ttgacgttcc accgccagaa gttgttcatc aggagccata aacagaaaac
     3541 cgccggggtt acccggcggt gcagattgtt gacaacgtac gcattgttca tgccggatgc
     3601 ggcgtaaacg ccttatccgg cctacaaaac agtgcaaatt caatatattg caatcttctg
     3661 gtaggcctga taagcatcgc gcatcaggca agcttacatt tgtcatcagt atcaaccacc
     3721 gggtaaccgg cggtgatggc tttttgacga gctacagaca aaaatgcatt cgtcattgat
     3781 tagcccggct agttgtcgct tttgccgtga ctgctcttgc cacctttttt acctgcttca
     3841 gatgcgcgct gcgggtcatt cttgaaatta cccccgctgt gctgtccacc ttttttacct
     3901 gcttctgatg ctctttcgcg gtcttctgca aaattgccgg aaccgcctcg atggtttgcc
     3961 attactgacc tccgtctggg tatagacatc atattgcata gaattaacgc ggcgtatggc
     4021 ggctgccata ttttcgctac gtgggactaa gcttagtgaa ctgacgcggg tagacccgcg
     4081 tttggtaata tcctgcaaca gcttcttagc gagaatagat atcatctcca ataattatcc
     4141 ataagccgct cttatgatta aaaaaacgcg atgagataaa aatgtgtcat tttgcgacaa
     4201 aattacgtgc ttgtgaaagt tgttataaat caaataagtg gttgtgaaat ttgcactctg
     4261 aaaaggacgt cttatcttta aataagtggt agcgaatcgc tacggaatag agataacacg
     4321 aggagtggtt agaaatggct aaagttctgg tgctttatta ttccatgtac ggacatattg
     4381 aaacgatggc acgcgcagtc gctgagggtg caagcaaagt cgatggcgca gaagttgtcg
     4441 ttaagcgtgt accggaaacc atgccgccgc aattatttga aaaagcaggc ggtaaaacgc
     4501 aaactgcacc ggttgcaacc ccgcaagaac tggccgatta cgacgccatt atttttggta
     4561 cacctacccg ctttggcaac atgtccggtc aaatgcgtac cttcctcgac cagacgggcg
     4621 gcctgtgggc ttccggcgca ctatacggaa aactggcgag cgtctttagt tccaccggta
     4681 ctggcggcgg tcaggaacaa actattactt caacctggac gacccttgcg catcacggca
     4741 tggtgattgt ccccattggc tacgcagcgc aggaattatt tgacgtttca caggttcgcg
     4801 gcggtacgcc gtacggcgca accaccatcg caggcggtga cggctcacgc cagccaagcc
     4861 aggaagaact gtctattgct cgttatcaag gggaatatgt cgcaggtctg gcagttaaac
     4921 ttaacggcta atcttcaaca ggaggatacg catgccaact caagaagcga aagctcatca
     4981 cgtcggtgaa tgggcatctc tgcgcaatac gtcgccggaa atagccgagg ccattttcga
     5041 agtcgccggg tacgacgaaa aaatggcaga aaagatttgg gaagaaggta gcgatgaagt
     5101 gttagtcaaa gcctttgcta aggccgataa agattcgctt ttttggggcg aacagaccat
     5161 cgaacgtaaa aacgtttaac cagcgactcc cccgcttctc gcgggggagt tttctgttat
     5221 ttcaccgctt cattcaacac gctatcaaat ttatccatcg ggcagaaacc gttggcatct
     5281 atcgggcaac cgcttaattc cagcgtcaca cgctgagcag gtgcctgcag ggttaacgca
     5341 tcggcattac gtaactgttc cgcactctga tacacatatt caattttcat caaatcgcga
     5401 ttggctttgc tgtcatgcca acgctggaaa acgattttgc cgccaatcgg cgtgcgttcg
     5461 ttctggtcat gcaactgata cggtttgaaa tccagcgccg ttaacagaga ggcaatgttg
     5521 gagtcgtgcc caaccaacac tgtaattttc ggtgcgctgg tgcgatcggt gaccagagct
     5581 ttgtcgatat aactgaccag cggtttcgca acattgcgcg ccacttccgg tgaggtaaac
     5641 aggctgtcct ggtagccgtt tttaagcttc gacaacacct tccactgctg gtcagatttg
     5701 atttctcccc aggccacctg atccatcgga aaaccttcgt aatattgcaa agtaaacgca
     5761 tctaccagcg agttgccgac tttcagcggc ccggaaacac ctggttcttg ttgatacttg
     5821 gcgctaaagg tatttttgcc atccaccagc gaacactgtt gtttctcttt acaggcaggg
     5881 gaatctttat agttaacgat tttttccagt agctggtagc tgtcggtaag ctggagtttg
     5941 ctgagctctt tctccattgc cgccaccgcc tgttcactga atgcggcgga atcatcggtg
     6001 atcaccgggt taaaggttgg gtccatagtg cccatttttt cctggtgatg cacaggaata
     6061 tcacaccccg ggaatgcgcc ggtaataaag aactgtgcgg tcgcaacggt acgttgcaga
     6121 ctattggcat aggcataaac ggtgtccggc ggcgggcatt cccccgattt caccatcccc
     6181 tgctctgcca gccattcacg catgtaatgg cccatataca cttcgagcac gccacctttg
     6241 gtggtgagtt gcccaccggg gacgtcccat tctggccatt tattcggcgt cgactgctcc
     6301 agcacactgc cattgttcgc cagcggcgca cgtaagttat ggcggctcat catgagcact
     6361 tgctgtagct gatagccttc cggtacggtt tgtgcctgag catttgaagc gagtaaaact
     6421 atccctgcca cagttgcggc gattagcgtt ttgttcattc ctggcacctc ttttgttatc
     6481 aatcattaaa gagtgtgaca gaaatatgac ttttttccgg gaacaggttc gcaaaatggc
     6541 ttgttgaacg cctgtttttc tatatatatc ttcaaaaaca ggcaaggttt gacgcaaact
     6601 atgcctgatt agcccctgcc tcgcgtacgc cgctgataat tgcacctgtg gctaaacaga
     6661 atatcagcgg cgttactaat acagaggcaa tagtaccaat aataagagaa gtgaggctaa
     6721 taacaatggt ttttagcaca tgcctttgtc attaaattgc ataacgagtt aaaaaatcaa
     6781 tttgcgcatt cctttcctta ttgtttaact cgtaagcctg agcaaaaagt tcaggaggga
     6841 ggtaaacgtc ataatgattg ccagtgttta ttaaataccc atactcaagg tcgtcatctg
     6901 ttgaaaagga ttcattccct aaggagattg atgccacatt gctgtaaaca ataaataatg
     6961 agcaaatgct atctgaaaga gcagtgagga aatttttcac ctcattaacc ttctgctctg
     7021 ggtcggtgtc taaaacaagg cgtgccttgt tatcattaaa tataaattcg ggtaaatgca
     7081 gtatgcattt tttgtcattc aa
//
LOCUS       DAZRUF010000211         7046 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZRUF010000211 DAZRUF010000000
VERSION     DAZRUF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.212"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            554..1543
                     /gene="araF"
                     /locus_tag="ACHXOL_003466"
     CDS             554..1543
                     /gene="araF"
                     /locus_tag="ACHXOL_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR6810361.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1613..3127
                     /gene="araG"
                     /locus_tag="ACHXOL_003467"
     CDS             1613..3127
                     /gene="araG"
                     /locus_tag="ACHXOL_003467"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR6810362.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3142..4128
                     /gene="araH"
                     /locus_tag="ACHXOL_003468"
     CDS             3142..4128
                     /gene="araH"
                     /locus_tag="ACHXOL_003468"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR6810363.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4295..5095
                     /gene="otsB"
                     /locus_tag="ACHXOL_003469"
     CDS             4295..5095
                     /gene="otsB"
                     /locus_tag="ACHXOL_003469"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR6810364.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5070..6494
                     /gene="otsA"
                     /locus_tag="ACHXOL_003470"
     CDS             5070..6494
                     /gene="otsA"
                     /locus_tag="ACHXOL_003470"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR6810365.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6501..6929)
                     /gene="uspC"
                     /locus_tag="ACHXOL_003471"
     CDS             complement(6501..6929)
                     /gene="uspC"
                     /locus_tag="ACHXOL_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR6810366.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1783 a         1632 c         1826 g         1805 t
ORIGIN      
        1 tttttgtatc ttgttgatat taatcaatga atttgaattc atgatgaagt gatgatttta
       61 taaaacgttt tttcattttt gcgagatggc tctcattata cgtgttctgt taattaatga
      121 gcacagtgat aattaatggc gaaatgaatg acgtgcattt tccacatctt tgagttgcgg
      181 ttattacacc atttcaaaaa ctcaacgcca ggtaatgcgg cctattgact ggttaaaaag
      241 aagacatccc gcatgggtac caaagacaac aaggatttcc aggctaatct tatggattaa
      301 tctgctgtgc attcgacaat ttgtctgaca aattggcttt cccttatgtc ttttcccgct
      361 aaatttatgc acgttctcac tgtaattctg cgatgtgata ttgctctcct atggagaatt
      421 aatttctcgc taaaactatg tcaacacggt cacttatctt ttagataaaa ggtaatgctt
      481 tgttttccga ttaatttaac gaaagtcatt cgtttttgcc ctacacaaaa cgacactaaa
      541 gctggagaga accatgcaca aatttactaa agccctggca gccattggtc tggcagccgt
      601 tatgtcacaa tccgctatgg cggagaacct gaagctcggt tttctggtga agcaaccgga
      661 agagccgtgg ttccagaccg aatggaagtt tgccgataaa gccgggaagg atttagggtt
      721 tgaggttatt aagattgccg tgccggatgg cgaaaaaaca ttgaacgcga tcgacagcct
      781 ggctgccagt ggcgcaaaag gtttcgttat ttgtactccg gaccccaaac tcggctctgc
      841 catcgtcgcg aaagcgcgtg gctacgatat gaaagtcatt gccgtggatg accagtttgt
      901 taacgccaaa ggtaagccaa tggataccgt tccgctggtg atgatggcgg cgactaaaat
      961 tggcgaacgt cagggtcagg aactgtataa agagatgcag aaacgtggct gggatgtcaa
     1021 agaaagcgcg gtgatggcga ttaccgccaa cgaactggat acggcacgtc gccgtacaac
     1081 gggttctatg gatgcgctga aagcggccgg attcccggaa aaacaaattt atcaggtacc
     1141 taccaaatct aacgacatcc cgggggcatt tgacgctgcc aactcaatgc tggttcaaca
     1201 tccggaagtt aaacattggc tgatcgtcgg tatgaacgac agcaccgtgc tgggcggcgt
     1261 acgcgcgacg gaaggtcagg gctttaaagc tgccgatatc atcggcattg gcattaacgg
     1321 tgtggatgcg ttgagcgaac tgtctaaagc acaggcaacc ggcttctacg gttccctgct
     1381 gccaagcccg gacgtacatg gctataaatc cagcgaaatg ctttacaact gggtagcaaa
     1441 agacgttgaa ccgccaaaat ttaccgaagt taccgacgtg gtgctgatca cgcgtgacaa
     1501 ctttaaagaa gaactggaga aaaaaggttt aggcggtaag taatttgccg gaaaaattcc
     1561 cctctgcatg atgcagaggg ggtgtgaacg accagtgatt cacggagacg ttatgcaaca
     1621 gtctaccccg tatctctcat ttcgcggcat cggtaaaacg tttcccggcg ttaaggcgct
     1681 gacggatatt agttttgact gctatgccgg tcaggttcat gcgttgatgg gtgaaaatgg
     1741 cgcaggaaaa tcaactctct taaaaatcct cagcggcaac tatgcgccaa ccacgggttc
     1801 tgtagtgatt aatgggcagg aaatgtcctt ttccgacacg accgcagcac ttaacgcggg
     1861 tgtggcgatt atttaccagg aactgcatct cgtgccggaa atgaccgtcg cggaaaacat
     1921 ctatctcggc cagctgccgc ataaaggcgg cattgtgaat cgctcattgc tgaattatga
     1981 ggcgggttta caacttaaac atcttggtat ggatattgac ccggacacgc cgctgaaata
     2041 tctctccatt ggtcagtggc agatggttga aatcgccaag gcgctggcgc gtaacgctaa
     2101 aattatcgcc tttgatgagc caaccagctc cctttctgcc cgcgaaatcg acaatctttt
     2161 ccgcgttatt cgtgaactgc gaaaagaggg gcgggtgatc ttatacgttt ctcaccgtat
     2221 ggaagaaata tttgccctca gcgatgccat cactgtcttt aaagatggac gttatgtcaa
     2281 aacctttacc gatatgcagc aggttgacca cgacgcgctg gtgcaggcga tggtcgggcg
     2341 cgacattggc gatatctacg gctggcaacc tcgtagttat ggcgaggagc gcctgcgtct
     2401 tgatgctgtg aaagcaccag gcgtgcgtac gccaataagt ctggcggttc gcagtggtga
     2461 aattgttggg ctgtttggtc tggtaggggc ggggcgtagc gaattaatga aaggcttgtt
     2521 tggcgggacg caaatcaccg ccggtcaggt ttatatcgac caacagccga tcgatattcg
     2581 taaaccgagc cacgccattg ccgcaggcat gatgctctgc ccggaagatc gcaaagcgga
     2641 aggcattatt cccgtgcact ccgttcgcga caatatcaac atcagtgcca gacgcaaaca
     2701 tgtgctcggc ggttgtgtaa tcaacaacgg ttgggaagaa aacaatgccg atcagcacat
     2761 tcgttcgctc aacatcaaaa cgccgggcgc tgagcaactg atcatgaatc tctcaggcgg
     2821 aaatcagcaa aaagccattc tgggccgctg gttatcggaa gagatgaagg tcattttgct
     2881 ggatgaacct acgcgcagca ttgatgttgg tgctaagcac gaaatatata acgtaattta
     2941 tgcgctggcg gcgcagggtg tggcggtgct gtttgcctcc agcgacttac ctgaagtcct
     3001 cggcgttgcc gaccggattg tggtgatgcg ggaaggtgaa atcgccggtg aattgttaca
     3061 cgagcaggca gatgagcgtc aggcactgag ccttgcgatg cctaaagtca gccaggcagt
     3121 tgcctgagta aggagagtat gatgtcttct gtttctacat cgggttctgg cgcacctaag
     3181 tcgtcattca gcttcgggcg tatttgggat cagtacggca tgctggtggt gtttgcggtg
     3241 ctctttatcg cctgtgccat ttttgtccca aattttgcca ccttcattaa tatgaaaggg
     3301 ttgggcctgg caatttccat gtcggggatg gtggcgtgtg gcatgttgtt ctgccttgct
     3361 tccggtgact ttgacctttc tgtcgcctcc gtaattgcct gtgcgggtgt caccacggcg
     3421 gtggttatca acctgactga aagcctgtgg attggcgtgg cagcggggtt gctgcttggc
     3481 attctctgtg gcctggtcaa tggctttgtt atcgccaaac tgaaaataaa tgctctgatc
     3541 acaacactgg caacgatgca gattgttcga ggtctggcgt acatcatttc agacggtaaa
     3601 gcggtcggta tcgaagatga aagcttcttt gcccttggtt acgctaactg gttcggtctg
     3661 cctgcgccaa tctggctcac cgtcgcgtgt ctgattatct ttggtttgtt gctgaataaa
     3721 accacctttg gtcgtaacac cctggcgatt ggcgggaacg aagaggctgc gcgtctggcg
     3781 ggtgtaccgg ttgttcgcac caaaattatt atctttgttc tctctggcct ggtatctgcg
     3841 atagccggaa ttattctggc ttcacgtatg actagtgggc agccaatgac gtcgattggt
     3901 tatgagctta ttgttatctc cgcctgcgtt ttaggtggcg tttctctgaa aggtggcatc
     3961 ggaaaaatct catatgtggt ggcgggtatc ttaattttag gcaccgtgga aaacgccatg
     4021 aacctgctta atatttctcc tttcgcgcag tacgtggttc gcggcttaat cctgctggca
     4081 gcggtgatct tcgaccgtta caagcaaaaa gcgaaacgca ctgtctgatg cttttttcca
     4141 caacaattta acgttttttc ccaccacagc cagccgccac aacggttggc tgttcttcat
     4201 tgcaaatggc gacccccgtc acactgtcta tacttacatg tctgtaaagc gcgttctgcg
     4261 caaaacaata agaaaagaga aggaggagag ccgggtgaca gaaccgttaa ccgaaacccc
     4321 tgaactatcc gcgaaatatg cctggttttt tgatcttgat ggaacgctgg cggaaatcaa
     4381 accgcatccc gatcaggtcg tcgtgcctga caatattctg caaggactac agctactggc
     4441 aaccgcaagt gatggtgcat tggcattgat atcagggcgc tcaatggtgg agcttgacgc
     4501 actggcaaaa ccttatcgct tcccgttagc gggcgtgcat ggggcggagc gccgtgacat
     4561 caatggtaaa acacatatcg ttcatctgcc ggatgcgatt gcgcgtgata ttagcgtgca
     4621 actgcataca gtcatcgctc agtatcccgg cgcggagctg gaggcgaaag ggatggcttt
     4681 tgcgctgcat tatcgtcagg ctccgcagca tgaagacgca ttaatgacat tagcgcaacg
     4741 tattactcag atctggccac aaatggcgtt acagcaggga aagtgtgttg tcgagatcaa
     4801 accgagaggt accagtaaag gtgaggcaat tgcagctttt atgcaggaag ctccctttat
     4861 cgggcgaacg cccgtatttc tgggcgatga tttaaccgat gaatctggct tcgcagtcgt
     4921 taaccgactg ggcggaatgt cagtaaaaat tggcacaggt gcaactcagg catcatggcg
     4981 actggcgggt gtgccggatg tctggagctg gcttgaaatg ataaccaccg cattacaaca
     5041 aaaaagagaa aataacagga gtgatgacta tgagtcgttt agtcgtagta tctaaccgga
     5101 ttgcaccacc agacgagcac gccgccagtg ccggtggcct tgccgttggc atactggggg
     5161 cactgaaagc cgcaggcgga ctgtggtttg gctggagtgg tgaaacaggg aatgaggatc
     5221 agccgctaaa aaaggtgaaa aaaggtaaca ttacgtgggc ctcttttaac ctcagcgaac
     5281 aggaccttga cgaatactac aaccaattct ccaatgccgt tctctggccc gcttttcatt
     5341 atcggctcga tctggtgcaa tttcagcgtc ctgcctggga cggctatcta cgcgtaaatg
     5401 cgttgctggc agataaatta ctgccgctgt tgcaagacga tgacattatc tggatccacg
     5461 attatcacct gttgccattt gcgcatgaat tacgcaaacg gggagtgaat aatcgcattg
     5521 gtttctttct gcatattcct ttcccgacac cggaaatctt caacgcgctg ccgacatatg
     5581 acaccttgct tgaacagctt tgtgattatg atttgctggg tttccagaca gaaaacgatc
     5641 gtctggcgtt cctggattgt ctttctaacc tgacccgcgt cacgacacgt agcgcaaaaa
     5701 gccatacagc ctggggcaaa gcatttcgaa cagaagtcta cccgatcggc attgaaccga
     5761 aagaaatagc caaacaggct gccgggccac tgccgccaaa actggcgcaa cttaaagcgg
     5821 aactgaaaaa cgtacaaaat atcttttctg tcgaacggct ggattattcc aaaggtttgc
     5881 cagagcgttt tctcgcctat gaagcgttgc tggaaaaata tccgcagcat catggtaaaa
     5941 ttcgttatac ccagattgca ccaacgtcgc gtggtgatgt gcaagcctat caggatattc
     6001 gtcatcagct cgaaaatgaa gctggacgaa ttaatggtaa atacgggcaa ttaggctgga
     6061 cgccgcttta ttatttgaat cagcattttg accgtaaatt actgatgaaa atattccgct
     6121 actctgacgt gggcttagtg acgccactgc gtgacgggat gaacctggta gcaaaagagt
     6181 atgttgctgc tcaggaccca gccaacccgg gcgttcttgt tctttcgcaa tttgcgggag
     6241 cggcaaacga gttaacgtcg gcgttaattg ttaaccccta cgatcgtgac gaagttgcag
     6301 ctgcgctgga tcgtgcattg actatgtcgc tggcggaacg tatttcccgt catgcagaaa
     6361 tgctggacgt tatcgtgaaa aacgatatta accactggca ggagtgcttc attagcgacc
     6421 taaagcagat agttccgcga agcgcggaaa gccagcagcg cgataaagtt gctacctttc
     6481 caaagcttgc gtaggagcgg ttaatctccc gtaagtggaa ctaacagcac atcgacctca
     6541 cttgaggcaa tcactctttt cgccgagcag gatgctcgcg aaaagaaact gtgattgtga
     6601 ttaccgcaaa tcaccaaatc gaaatgatgt ttgtgacata cttccagaat atgttcgctt
     6661 aactcaccgt aggcaataaa cgtcttgtca acgggatacc ccgcatcctg aattaactta
     6721 tcaagaaagc tttgcgtttc ttcttgcatc acgctgcgta aatcttcaag catcggcgcg
     6781 gctaattgat tgtacatttc cgggtcggaa gcgagagtaa ttaaactgat gtgtcccttt
     6841 actggcctgg cgatagatac cgcttttgcc agcagttgct gactttccgg tgtaaccgca
     6901 acagcgacaa gaatattgct atagctcata acctgttcct tattctgtga acttcaggtg
     6961 acattaaagc tttactccta atggagaaac gacgcaatcc caactcggtc aaacaggtaa
     7021 atattagtct gattattagt aaagac
//
LOCUS       DAZRUF010000212         7035 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZRUF010000212 DAZRUF010000000
VERSION     DAZRUF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.213"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..245
                     /gene="yafQ"
                     /locus_tag="ACHXOL_003472"
     CDS             <1..245
                     /gene="yafQ"
                     /locus_tag="ACHXOL_003472"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR6810367.1"
                     /translation="SKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSW
                     KGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            401..1141
                     /gene="dpaA"
                     /locus_tag="ACHXOL_003473"
     CDS             401..1141
                     /gene="dpaA"
                     /locus_tag="ACHXOL_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR6810368.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1112..1879)
                     /locus_tag="ACHXOL_003474"
     CDS             complement(1112..1879)
                     /locus_tag="ACHXOL_003474"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR6810369.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2085..2663)
                     /gene="lpcA"
                     /locus_tag="ACHXOL_003475"
     CDS             complement(2085..2663)
                     /gene="lpcA"
                     /locus_tag="ACHXOL_003475"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR6810370.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2903..5347
                     /gene="fadE"
                     /locus_tag="ACHXOL_003476"
     CDS             2903..5347
                     /gene="fadE"
                     /locus_tag="ACHXOL_003476"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR6810371.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5390..5836)
                     /gene="ivy"
                     /locus_tag="ACHXOL_003477"
     CDS             complement(5390..5836)
                     /gene="ivy"
                     /locus_tag="ACHXOL_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR6810372.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6017..6787
                     /gene="yafV"
                     /locus_tag="ACHXOL_003478"
     CDS             6017..6787
                     /gene="yafV"
                     /locus_tag="ACHXOL_003478"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR6810373.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(6829..>7035)
                     /locus_tag="ACHXOL_003479"
     CDS             complement(6829..>7035)
                     /locus_tag="ACHXOL_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR6810374.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         1643 a         1791 c         1882 g         1719 t
ORIGIN      
        1 tttcaaagga tgtaaaactt gcacaaaagc gtcataagga tatgaataaa ttgaaatatc
       61 ttatgacgct tcttatcaat aatgctttac cgcttccagc tgtttataaa gaccacccgc
      121 tgcaaggttc atggaaaggt tatcgcgatg ctcatgtcga accggactgg atcctgattt
      181 acaaacttac cgataaactt ttacgatttg agagaactgg aactcacgcg gcgctctttg
      241 ggtaaagcac gctgccgatg aaatttgtaa accctgcgcc gacaggcttg cgtaaaactc
      301 ttttggtgat aatgtgagcc ttccaaatcc agactattcc gatttgggat ctattttgcc
      361 gtcctggcgt gtgtaaccgt tttatcaagg aataagcagt atgcgtaaaa tcgcattaat
      421 tcttgcgatg ttgttgatcc cgtgcgtttc gtttgccggt ttgctgggca gcagtagctc
      481 gaccacgcca gtgagcaaag agtataagca gcagttgatg ggatcccctg tctacatcca
      541 gatcttcaag gaagaacgta cgctcgatct ctacgtcaaa atgggcgagc aatatcagct
      601 gctcgacagt tataaaatct gtaaatattc cggcggctta gggccaaaac agcgtcaggg
      661 cgatttcaaa agcccggaag ggttttatag cgtccagcgt aatcagttaa aaccagacag
      721 ccgttactac aaagcgatta atattggttt ccccaatgcc tatgaccgtg cgcatggtta
      781 tgaagggaaa tacctgatga tccacggcga ttgtgtttcc atcggctgct acgcaatgac
      841 caatcagggt attgatgaga tcttccagtt cgttactggt gcgctggtgt ttggtcagcc
      901 gagcgtgcaa gtgagtattt acccgttccg catgaccgac gccaatatga agcgccataa
      961 atattccaac tttaaggact tctgggagca actgaagccg ggttacgact actttgagca
     1021 aacccgtaag ccaccgaccg tttctgtggt caatggtcgt tacgtggtca gcaagccgtt
     1081 aagccacgaa gttgtgcagc cacaactggc atcaaactac acgctccccg aggcaaaata
     1141 agcgccattc gcctggcata atcttttgcc aggtttcatt gcccgtcagc ggctgtgttg
     1201 caatcaccgt gaccacatca ttcggtgtgg tctgcgagct gaagtcgatt tccacatcct
     1261 gatccagcag cgttgccacg ccaaacggcg cgcggcgggt gatccagtgt aaattagtcg
     1321 agcaatacgc catcacatag cgcccgtcag aaagcagcat gttgaaaacg cccttctgcc
     1381 gcagttcatc cgccagtgag gcgatatatt taaacactgc cgccatgttg cccggtgtgc
     1441 gcgggtaacg ctgcgttaat ttatgcagga gccagcagaa ggctttttcg ctatcggttt
     1501 caccgaccgg gcggaagttg ccggtttcca gtgatttgta gcccgtcagt tgtccgttat
     1561 gggcataagt ccagttacgc ccccataact cacgggtaaa cgggtgagta ttttccagcg
     1621 ccacctcgcc ccgattggcc tggcgaatat gagccaccac cgaacaggat tttatcgggt
     1681 agtcctggac aagtttggcg atgggggaat taaagctggg ttgtggatct ttaaatgtgc
     1741 gacagccttt accttcgtaa aaggtaatgc cccagccatc tttatgtggc ccggttccac
     1801 caccacgctg caccagaccg gtgaaactaa agcagatatc ggtagggacg ttggcgctca
     1861 tcccgagcaa ttcgcacatg ggtaacctcc acaacagcgg gagcgcatgg cccccggcga
     1921 agattgattt aagaattttc aggccggatg cggcgtaaac gtcttattcg gcctacggtt
     1981 cgcgtacaga tttgtaggca tgataagacg catcagcgtc gcatcaggca tcagcgcaca
     2041 agtgccggat gcggcgtaaa cgccttatcc ggcctacgcc agacttactt aaccatctct
     2101 ttttcaatta actggatcag gatatggatc actttaatgt gaatctcctg aatgcggtcg
     2161 gcataaccaa agtgcggaac gcgaatttcg atatccgccg tgccagccat tttaccgccg
     2221 tctttaccgg tcagggtgat cactttcatc cccttctcac gcgccgctgc gatcgctttg
     2281 atcacgtttg cagagttacc ggaggtggag atccccagca gtacatcgcc ttcgcgacct
     2341 accgcttcaa cgtagcggga gaaaatatca ttgaaaccga aatcattacc gacgcaggaa
     2401 atatgactaa cgtcagaaat agcaatcgcc gggtagcccg gacggttttc acggtagcga
     2461 ccggtcaact cttcggcaaa gtgcatagcg tcgcaatggg aaccgccgtt gccgcaggaa
     2521 agcactttgc caccggcttt aaagctgtct gctaacagga ccgccgcgcg ctgaatggcg
     2581 tgaatattgg cgtcatcttt taaaaagtta gccagcgttt ccgccgcttc gttcagttcg
     2641 ttacgaataa gatcctggta catgaggata tccttcagca taaatgtaat agacaaaatg
     2701 cagtgtaccg gataccgcca aaagcgagaa gtacgggcag gtgctatgac caggactttt
     2761 tgacctgaag tgcggataaa aacagcaaca atgtgagctt tgttgtaatt atattgtaaa
     2821 cacattgcta aatgttttta catccactac aaccatatca tcacaagtgg tcagacctcc
     2881 tacaagtaag gggcttttcg ttatgatgat tttgagtatt ctcgctacgg ttgtcctgct
     2941 cggcgcgttg ttctatcacc gcgtgagctt atttatcagc agtctgattt tgctcgcctg
     3001 gacagccgcc ctcggcgttg ctggtctgtg gtcagcgtgg gtactggtgc ctctggccat
     3061 tatcctcgtg ccatttaact ttgcgcctat gcgtaagtcg atgatttccg cgccggtatt
     3121 tcgcggtttc cgtaaggtga tgccgccgat gtcgcgcact gagaaagaag cgattgatgc
     3181 gggcaccacc tggtgggagg gcgacttgtt ccagggcaag ccggactgga aaaagctgca
     3241 taactatccg cagccgcgcc tgaccgccga agagcaagcg tttctcgacg gcccggtaga
     3301 agaagcctgc cggatggcga atgatttcca gattactcat gagctggcgg atctgccgcc
     3361 ggagttgtgg gcgtacctta aagagcatcg cttcttcgcg atgattatca aaaaagagta
     3421 cggcggactg gagttctcag cttatgccca gtctcgcgtg ctgcaaaaac tctccggcgt
     3481 gagcgggatc ctggcgatta ccgtcggcgt gccgaactca ttaggcccgg gcgagctgct
     3541 gcaacattac ggcactgacg agcagaaaga tcactatctg ccgcgtctgg cgcgtggtca
     3601 ggagatccct tgctttgcac tgacaagccc ggaagcgggt tccgatgcgg gcgcgattcc
     3661 ggacaccggg attgtctgca tgggcgaatg gcagggccag caggtgctgg ggatgcgtct
     3721 gacctggaac aaacgctata ttacgctggc accgattgcg actgtgcttg ggctggcgtt
     3781 taaactctcc gacccagaaa agctgctggg tggcgcagaa gatttaggta ttacctgtgc
     3841 gctgatcccg accaccacgc cgggcgtgga aattggtcgt cgccacttcc cgctgaacgt
     3901 accgttccag aacggaccga cgcgcggtaa agatgtcttc gtaccgatcg attacatcat
     3961 cggtgggccg aaaatggccg ggcaaggctg gcggatgctg gtggagtgcc tctcggtagg
     4021 ccgcggcatc accctgcctt ccaactcaac cggcggcgtg aaatcggtag cgctggcaac
     4081 gggcgcgtat gctcacattc gccgtcagtt caaaatctct atcggtaaga tggaagggat
     4141 tgaagagccg ctggcgcgta ttgccggtaa tgcctacgtg atggatgctg cggcatcgct
     4201 gattacctac ggcattatgc tcggcgaaaa acctgccgtg ctgtcggcta tcgttaagta
     4261 tcactgtacc caccgcgggc agcagtcgat tattgatgcg atggatatca ctggtggcaa
     4321 aggcattatg ctcgggcaaa gcaacttcct ggcgcgtgct taccagggcg caccgattgc
     4381 catcaccgtt gaaggggcta acattctgac ccgcagcatg atgatcttcg gacaaggagc
     4441 gattcgttgc catccgtacg tgctggaaga gatggaagcg gcgaagaaca atgacgtcaa
     4501 cgcgttcgat aaactgttgt tcaaacatat cggtcacgtc ggtagcaaca aagttcgcag
     4561 cttctggctg ggcctgacgc gcggtttaac cagcagcacg ccaaccggcg atgccactaa
     4621 acgctactat cagcacctga accgcctgag cgccaacctc gccctgcttt ctgatgtctc
     4681 gatggcagtg ctggacggca gcctgaaacg tcgcgagcgc atctcggccc gtctggggga
     4741 tattttaagc cagctctacc tcgcctctgc cgtgctgaag cgttatgacg acgaaggccg
     4801 taatgaagcc gacctgccgc tggtgcactg gggcgtacaa gatgcgctgt atcaggctga
     4861 acaggcgatg gatgatttac tgcaaaactt cccgaaccgc gtggttgccg ggctgctgaa
     4921 tgtggtgatc ttcccgaccg gacgtcatta tctggcacct tctgacaagc tggatcataa
     4981 agtggcgaag attttacaag tgccgaacgc cacccgttcc cgcattggtc gcggtcagta
     5041 cctgacgccg agcgagcata atccggttgg cttgctggaa gaggcgctgg tggatgtgat
     5101 tgccgccgac ccaattcatc agcggatctg taaagagctg ggtaaaaacc tgccgtttac
     5161 ccgtctggat gaactggcgc acaacgcgct ggcgaagggg ctgattgata aagatgaagc
     5221 cgctattctg gtgaaagctg aagaaagccg tctgcgcagt attaacgttg atgactttga
     5281 tccggaagag ctggcgacga agccggtaaa gttgccggag aaagtgcgga aagttgaagc
     5341 cgcgtaattg aataacggag ccgaaaggct ccgtttcttt atccgctaat tatttaaaat
     5401 taaagccatc cggatggttt tccaggctgc cggtcaacgc cgcgaacagc accgttttac
     5461 catcaatcga aagcgcatcg ttcacattca gccaggtgag tttctcttgc gacgttttct
     5521 catcaatagt cgagaacagc cccgtcatct gattagattt ctcggaccac atcacagcga
     5581 tacgttgcga gccacagtca tgcggtttgc acgcgctcat cacctgatac gtatcatctc
     5641 ccaacgttac ggtttgtgcg ggagtataag taccgccttt catcacccag gcaggcagct
     5701 tatgcccttg taccatctga ttaaatgcag ctttggtggt ttcgcccttt gcaaggctgc
     5761 taatggttaa atcatcctgc gccattgcac tggtggcgat gaccagagcg gcgactgtcg
     5821 ttattgtctt aaacatcatt cctcccgagc ttatcctgcc catgttatta accttctaaa
     5881 gcctggcgat aacacgataa tttgcaaatc agatatttcc aatatcagca tttgttatca
     5941 atttattctg cgctaaggca actgcctgat aaggtgttaa agttataaaa cccttcgcat
     6001 aaaggagttg tacaccgtgc ctggtttgaa gattacgctt ttgcagcaac cactggtgtg
     6061 gatggatggc cctgccaacc tgcgtcattt tgatcgtcaa ctggaaggta ttaccgggcg
     6121 cgatgtgatc gttctaccga agatgtttac cagcggcttt gccatggaag cggcagcttc
     6181 gtcgctagca caagatgacg tagtggactg gatgacagct aaggcgcagc agtgcaatgc
     6241 gctgattgca ggcagtgttg cattacaaac ggagtctggt tcggttaacc gctttttgct
     6301 ggttgagccg ggcggcacgg tacattttta tgataagcgt catctgttcc gcatggcaga
     6361 tgagcatcta cattataaag cgggcaatgc gcgagtgatt gtggaatggc gcggctggcg
     6421 tattttgccg ttggtgtgct acgacttacg ttttccggtg tggtcgcgca atctcaacga
     6481 ttatgacctc gccatctacg ttgccaactg gcctgctccg cgctctctgc actggcaggc
     6541 attgctgacg gcccgcgcga ttgagaatca ggcgtatgtg gcgggatgca atcgcgtcgg
     6601 cagcgatggc aacggctgcc attatcgcgg tgacagccgg gtgattaatc cacaaggcga
     6661 gattatcgct actgccgacg cgcatcaggc aacgcgcatt gatgcggagc tgtcgatgat
     6721 ggcattgcgg gaatatagag aaaagtttcc ggcatggcag gatgcggatg agtttaggtt
     6781 gcggtgagtg ggggttgtgt ttaaggctgg gggagagtcg gggtaagact acgaaagccc
     6841 gctctccgca aggactgacg ccagatagtt tctgtccata gctgcttttc gcatcttacg
     6901 tcttaaccct gccttgaata ccttatcatt cgtcaaaata ttaatagcga tgtgccgtat
     6961 ccctgaaaat aattctgctg catttcctct tcttattttg cagtcgtctt cattcattac
     7021 cacgtccaga cgcca
//
LOCUS       DAZRUF010000213         7020 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZRUF010000213 DAZRUF010000000
VERSION     DAZRUF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.214"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            108..605
                     /gene="ftnA"
                     /locus_tag="ACHXOL_003480"
     CDS             108..605
                     /gene="ftnA"
                     /locus_tag="ACHXOL_003480"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR6810375.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(643..882)
                     /locus_tag="ACHXOL_003481"
     CDS             complement(643..882)
                     /locus_tag="ACHXOL_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR6810376.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1073..2284
                     /gene="tyrP"
                     /locus_tag="ACHXOL_003482"
     CDS             1073..2284
                     /gene="tyrP"
                     /locus_tag="ACHXOL_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR6810377.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="ACHXOL_003483"
     CDS             complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="ACHXOL_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR6810378.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3207..3293)
                     /locus_tag="ACHXOL_003484"
     tRNA            complement(3207..3293)
                     /locus_tag="ACHXOL_003484"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3257..3259),aa:Leu,seq:taa)
     gene            complement(3306..3379)
                     /locus_tag="ACHXOL_003485"
     tRNA            complement(3306..3379)
                     /locus_tag="ACHXOL_003485"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3345..3347),aa:Cys,seq:gca)
     gene            complement(3434..3509)
                     /locus_tag="ACHXOL_003486"
     tRNA            complement(3434..3509)
                     /locus_tag="ACHXOL_003486"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3474..3476),aa:Gly,seq:gcc)
     gene            complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="ACHXOL_003487"
     CDS             complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="ACHXOL_003487"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR6810379.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="ACHXOL_003488"
     CDS             complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="ACHXOL_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR6810380.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="ACHXOL_003489"
     CDS             complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="ACHXOL_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR6810381.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1702 a         1806 c         1662 g         1850 t
ORIGIN      
        1 attcgtattt ttaataattg aagtttatat tttacctttt gcaaataata aaataacaaa
       61 ttatagtgac gccacagcaa caaatataac ctttgtggag cactatcatg ctgaaaccag
      121 aaatgattga aaaacttaat gagcagatga acctggaact gtactcttca ctgctttatc
      181 agcaaatgag cgcctggtgc agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc
      241 gtcacgccca ggaagagatg acgcatatgc agcgtctgtt tgattacctg actgataccg
      301 gcaatttacc gcgtattaat accgttgaat ctccgtttgc tgaatattcc tcacttgatg
      361 aattattcca ggaaacctat aaacacgaac aattaatcac ccagaaaatt aacgaactgg
      421 ctcatgctgc aatgaccaat caggactacc caacatttaa tttcctgcag tggtatgttt
      481 ctgagcagca tgaagaagag aaactgttca aatcgattat tgataaatta agcctggcag
      541 gcaaaagcgg cgaaggtctg tattttatcg acaaagaact ctctaccctc gacacacaaa
      601 actaatgctc tccggcggca ggtttactgc cgccgtttca aatcagtgac ggcaaatctt
      661 actctgatcg gtcgaaaaac cgtctttcga aggtataaat ttgccttttg ctgccagaaa
      721 cgctaccagc tctcccgctg tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc
      781 accaaaacgc gctttaatcg cagcttccag actggcatgc gtatattgct cgcctgattc
      841 aatcatcata tttaacactt catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa
      901 gcctagcgta gcgattgccg cttatgaaga ctttgcgcca gcgcaggact gaatgctttt
      961 tattgtacat ttatatttac actatatgta acgtcggttt gacgaagcag ccgttatgcc
     1021 ttaacctgcg ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa
     1081 cagaaccctg ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc
     1141 aatgccgctg gctgcggccg gtgttggttt tagcgttacg ttaatcttgt tgattgggct
     1201 ttgggcgttg atgtgctaca cggcgctatt actgctggag gtgtaccagc atgttccggc
     1261 agataccggt ctgggcacgc tggcaaaacg ctatctggga cgctacggtc aatggctgac
     1321 gggcttcagt atgatgttct taatgtatgc tctgactgcg gcatacatca gcggtgtcgg
     1381 tgaattgttg gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg
     1441 cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt gttggaacat cgctggtcga
     1501 tttatttaac cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt
     1561 gctgctaccg catattcaca aagtgaatct tttaaccctg ccgttgcaac aggggctggc
     1621 tctgtcagca atcccggtga tttttacctc atttggtttt cacggtagcg tgccgagtat
     1681 tgtcagctat atggatggca acgttcgtaa gctacgctgg gtgtttataa ccggtagtgc
     1741 gatccccctg gtggcatata ttttctggca ggtggcaacg cttggcagca ttgattcaac
     1801 aacctttatg ggattgctgg ctaatcatgc tggattaaac gggctgttac aggcgttacg
     1861 cgaaatggtg gcctctccgc atgttgagct ggcagtgcat ttatttgctg atttagccct
     1921 cgccacgtca tttctcggcg ttgcgttagg cttatttgat tatctggctg atttatttca
     1981 gcgttcaaat accgttggtg gacggttgca aactggtgca attacctttc tgccgccgtt
     2041 ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc
     2101 gctggcggta ctggcattga ttatcccttc gctgttgacc tggcaaagca gaaagcacaa
     2161 tcctcaggcg ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg
     2221 tggtattgct gtgattggcg tgcaattttt gattgcggca gggttgttac cagaagtggg
     2281 gtgatcagat agcctcaaat tccttattgg gtgccagaat taacgctgac acccaatttg
     2341 gcctcttaat gcaggcagca ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg
     2401 ttacgccccg gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc
     2461 gccatccagt acgcatgtag atcaagcgcc gccagtcgaa tggcatctac gctctcttca
     2521 aacgcttctg gcgacatctt ttctacccgc tcgaagtttt cctcagtacc gtgcagcgca
     2581 atcgcctcca gcgctggttt taacgaatcg ggcaacgttg accagtcaga aagtgccacg
     2641 ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc atcaacttct
     2701 cgcaagccga ataacggctc aaactgctcc gggaattcgt tcagacgctc tgcggtatcg
     2761 gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccagcgc
     2821 ggcacatagt cagccccacc ccacacggca accagccact gttccggttc aatctcttgc
     2881 ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag gatggcgtgg
     2941 ttagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt
     3001 cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg
     3061 gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt
     3121 aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg
     3181 ctttgatttt attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg
     3241 cgaacgccga gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa
     3301 gaaagtggag gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata
     3361 accgctttgt taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg
     3421 atgtctttta aactggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
     3481 tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa tcacttgatt
     3541 ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc
     3601 gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga
     3661 tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt
     3721 cagtaccgca gccacaaaga aaagtgcaat accggcgtac tcaacccaaa tgttcggacg
     3781 ccacagcagc catgccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga
     3841 gacagccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat
     3901 aatttcacgg gcgatcatcg ttgctgccgg taaggtcacc caccagctgt ggtaatgctc
     3961 ggttaccagc accatggcga tagccacgag aactttatct gctacagggt caaggaaagc
     4021 accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac
     4081 cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa
     4141 gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt
     4201 aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag
     4261 agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc
     4321 acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc
     4381 aacaacattt gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt
     4441 ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga
     4501 atatgctgga taacatgcag cgcgggagaa tctggcggca aactgaatcc ctcaccttcc
     4561 ggctcaaaga acagcgtttc cagcccagcc ttacgatctg ctcctttggc aacgccaagt
     4621 agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc
     4681 gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg
     4741 gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg
     4801 atgcctgtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc
     4861 acacaggaag cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc
     4921 ttcacttccg gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat
     4981 tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa
     5041 cgcgccctat cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa
     5101 agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt
     5161 aaggtgcgca tctggctgcc ttgtaaatag aactggccta cgaaggtttc taccacctcg
     5221 ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc
     5281 tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg
     5341 tcgaggtcgt cgccggtatt ggaaacgaat tgtttttcgg tgacgcgtcg caccgcctga
     5401 atttggtcgc gaatacgtgc agcttcttca aactccagat tctggctggc agtttccata
     5461 cgactaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac
     5521 tcaacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag
     5581 cgccctatct gatattgcag acacggacgc gagcgattgc gataaacact attttcgcac
     5641 tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc
     5701 gggaacgggc cgaaatattc acctttggca tgcttcgcac cacgatgcat cgccagacgc
     5761 gggtgggtat cgccactcag gaagataaaa ggataagatt tatcatcgcg tagcaaaacg
     5821 ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct
     5881 gtatgcgtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc
     5941 gaagcgaggt tgctgcggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg
     6001 acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg
     6061 gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat
     6121 tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt
     6181 tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg
     6241 agatctcatt gaccttctgg cccttggtga tcatcagcat aatctgcaat tcacgttcag
     6301 acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt
     6361 gagcgatatc agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga
     6421 cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc accggcctgc atgactttcg
     6481 ctggtaaagg gttttctgta tggacggtaa gcatgatgat tttgacatca gctgtggaac
     6541 gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca
     6601 ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctcac
     6661 cgacgacttt tatacccttt atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt
     6721 cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc
     6781 tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa
     6841 tggtgggtat tcgatttttc tctcgttttt gtaaattcga ttgtccacag ttatggtcac
     6901 gcccgtcacc aacggcttcc gggatcgttt cacagtaagc ccggcgatga aatgtgacaa
     6961 aaattgactt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac
//
LOCUS       DAZRUF010000214         6948 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZRUF010000214 DAZRUF010000000
VERSION     DAZRUF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.215"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..2777)
                     /locus_tag="ACHXOL_003490"
     CDS             complement(<1..2777)
                     /locus_tag="ACHXOL_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR6810382.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLP"
     gene            complement(2834..5205)
                     /locus_tag="ACHXOL_003491"
                     /pseudo
     CDS             complement(2834..5205)
                     /locus_tag="ACHXOL_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(5243..6859)
                     /locus_tag="ACHXOL_003492"
     CDS             complement(5243..6859)
                     /locus_tag="ACHXOL_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFR6810383.1"
                     /translation="MRKNNKTSVLLIIIILAMILGVVKIQVWLNDWNNDFFNALSQKE
                     TDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIRWREWLTDYYLNRWFADKNYYFTQ
                     IYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQSLSMLITFTVILWQSAGTLSFTV
                     GGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRPLNVEKQRSEATFRTNLVQHNKQT
                     ELIALSNAESLQRQELRDNFHTIKENWHRLMNRQRWLDYWQNIYSRSLSVLPYFLLLP
                     QFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPT
                     NKPKNCQHAVQVANASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSH
                     CWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICKALPLPVDDKSLSEVLHQVGLGKL
                     AARIHDHDRWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVRE
                     KLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1797 a         1588 c         1565 g         1998 t
ORIGIN      
        1 ggcaataata ctccagtata agtatttacc ggatgagaaa gatattgttt aacggcagtg
       61 ttaacattct ctaccgtcat ttgtttcaac aattgctcct gctcagtcca tgctgcagga
      121 tcgtcatatt gaataagact atttacaata gtgttcgcta attgttgaac gctacgctgt
      181 tggatatcga ggctgcgctg aacgttttgc tggtattcat tcagttcttg ctcactgatc
      241 cctttagcca gacgcttaac catcacttca ttcgctaacg ttaacagttc atcatgtcgt
      301 tctggttgac aagtaaaagc cagcaaatga ctgatatctt tggcctgagg atcaaccgag
      361 aggcgagaag aaacgctgta tgctccagat gcctgttcac gaatattaac gcgtagatct
      421 tttgccagtg cgacgttaaa agcatcgagc gccatacgcg tcgccagatt aacaggtgtc
      481 cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag gttcattttg ttcttttaca
      541 gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac ctgcggctaa tggcgaatca
      601 gagtgtttga ttgatcctaa gtaacgcgta attaacgcca cgagtttgtc ttctgagaca
      661 ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa acaactggcg atcggcagcc
      721 agcgcatctg cggcagtaaa ctgtacaatc tgattttctt gcagtaattt cgtgcggtca
      781 tcagcatagc gcgtctcata catctgctgg gcgaatttct ccgctggacg ctggtcgagc
      841 gttttcaacg cctgaatttg agcattttgt agcgatgccc aaatattgtc gttaatcgtg
      901 ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt cagggttatt agtccgcgcg
      961 ctaacggaga gcaacgtatt catgccactg actttactgc tcatggttac cgaattttcc
     1021 gcactccagc gtttcaggct ggaagaggag agctcaccaa cgccgcttcc gcttactgct
     1081 ttatttgcca gcgcgataag tgatttttgc tgcgcaggga aacttaaatc gcctttatta
     1141 gatacggcag taatttgtag cttttgctct tcacccgcgg attttgccag aataaccctg
     1201 gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca gcgtttcttt gctgctaatt
     1261 tccgcctgtg ggtcagcgtc tactgttaac gataaatttc tgcctgggaa gatgtaagcc
     1321 gccagctttt tgttggcgta ctctttttcc agcgccagga tagccgcaag agacaatgct
     1381 tttttggcgg caagctcatt gtttaccatt tgctcccaaa atgcgtcctg gttctttctt
     1441 aactgctgcc atttttccgc cagactttgc acggtaattt gctgccacag acgtttcgaa
     1501 agttgatatg tctcttccgg cgacaagaac ggcgtattat ttaatgagct ggatgccagg
     1561 cgactggtca gcatacgtaa atcacgttca gcttgctgat caaccgcatt tttcagccag
     1621 gtgaggcggg tagatttgac atcatcgagt tcttcagcag aaaaaccatg ctgatcaatg
     1681 gttgccaact ctgccattaa tgcatttgca gcatcctgca tattatcgtc tcgtgcattt
     1741 acacggaaaa acagcgactg ataatcgggt gcaattttaa cgctgcgcgc agtgccgcca
     1801 gaaatagtct tcaactcgcc cgactgtatg cgttcctgca gacgttgatt gaacagctga
     1861 actaacatgc tccattcagc ttgttcgaca aagctttgct catcgttcac ttgtaccatt
     1921 ggcaggcgat aatagagtgc gatgccgttc actcggtttt ctttatcatt aatgatatta
     1981 aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat tttcagctgc tttgttagcc
     2041 ggaagcttac ttaaattatc ctttatcagc gccagcgctt ctttactgtc gatatcgccg
     2101 accacgataa aggtcatatt atttggttga taccagcgtt gataaaattg gcgcaattgt
     2161 gccggtgtga ccgtggcgac agtatccatc aggccgatag gttcacggtc taaattacgg
     2221 gtatttgcca gcaggaaagg gcggcgcgcc tgagaggtgc gccatttcgc atcctgatgg
     2281 gcacgccatt cctcagtaat tacgccgcgt tcagcgtcta cttcgagtat ttcaaaggtt
     2341 gcggcattac tccattcact gaagattgcc atcacttgtt gcagattttg tttctgcgta
     2401 gtcggcagac tcacctgata cagcgtttcg tcatagctgg tataggcatt aacatcgcga
     2461 ccgaaacgca ggcccattga ctcaaatgtt tcgatgactt tattacccgg ccatgttttt
     2521 gtgccgttaa acatcatatg ttctacaaaa tgagccacgc cgcgctcatt gtcttcttcc
     2581 tgcaatgaac cggtatgaat ttgcagccat aaatttacct gatcctttgg ttgagcatgc
     2641 ggataaatca tatatcgcaa gccattgtcc agttgcccgg taattaactt ttcatcctgc
     2701 ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag ttgccactaa cgtcagtaag
     2761 aaacagaggt ttctcataat tatctccatg cgaaaaccgg gcgaatttac ccggttaagt
     2821 aaaatccgaa ctattaaaat ttcatgctga catcaagcca gaaagtacgg ccactggcgt
     2881 aggtcgccac accggtgttc gttgtatcaa cagcggtttt gctatccagt acattgagaa
     2941 tatcggcact gatcgtcagg ttttgttgtt tcagaaattg tggcgtccag gacaagcggg
     3001 tgtcccaggt caggctgcta tccagttttt cgtcaacata ctgcttgtaa tctgaatatt
     3061 cgctgatgta ttgcgcattt gtcttaccaa ggataatgcg agctttacgc gcttcttgcc
     3121 aggccagcgt attcgcccac actaacccgc tcggttgatg cgtgaaatcc atgtttaagg
     3181 agatctttaa tgggttatta aaatctgcca ctggaacact atcgtaagag accagattac
     3241 cgttataaac cacctggtta tcaccggtat tgctctcctc ataaccattg tttaacgaca
     3301 agttgccctt gctcttgata tagctaaaga caatttgtgg gttaatatct acttggcgga
     3361 tatgcagggg ttcggccagt tcaaaactga ggctgaacga atgcgttttg gttttgccat
     3421 cgttgttata ttcagtaatg gtggttttag tcgcgctgtc ggtacgactg cttttgctga
     3481 tttgatcatg cgcttcacgg taaacatagt ttgcacgcgc aataacgttc ttaccgattt
     3541 tctgctgcaa tcccattgcc agttcatcgt tataaggcgt tttcaaatcc tgataacgcg
     3601 tcagagtttt attacctgat accgattccg tccagctatt gcggatatca cgtaatccca
     3661 tatcaagaat attcccgcca tagtaacggt tataaccggc ggtaatcatt gaggtttgat
     3721 cagcaaaaat atcccattcc gtcataaagc gcggggagat attgtggttt gacagatagt
     3781 tgtcatagtc ataacgcaca ccgggcatta acgacacatt ccgccagcta atgtgctccg
     3841 ccatatacag cgtgtagttg tcaattccca ggctgccttt acctttatgg taaatggtat
     3901 ggttagtttt ctttcctgca gcattaatca cataggattc agactggtta tggcgttcag
     3961 tccatgcatc ggaatagatg tattccgcgc cgaagtaggg ttgatgcgaa acatcaccca
     4021 cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta attatctaca gcctgggaaa
     4081 tgtgtcctaa tccgccacag gtacaacgcc ctgtaatatc accatatgta catgaaagtt
     4141 cggtgtacca gatgtcatga tcgtgacggg tataatcact aatatgatcc caaccaacgg
     4201 tggtacgtag tttggcccat gcgagttgcg tatccatatc ccatgccaga ccataggatt
     4261 tattacccat ttcgcgatca gactgcggga aggtgctggt attataatca cggctggagc
     4321 cggtatattt taaggttaaa tcgtgggtaa agcggtcgct ggcaaaccag gtaaatttgc
     4381 tcaatgcagt atcgataacg tttttatact gtgcccgacc ggtgacaatg ccgtcattcg
     4441 aaacataatc cgcgcgggtg atatcagact ggcggcgcga taaaccggcg gtaacgccga
     4501 agttatcagc gagttcctga ttaaacgaca aggtataaaa gttcttttta aaatctgggg
     4561 agtaataagt acttcctgaa gagccttgat taaatgcgct cttgttattc tcatcgatat
     4621 gcgatgttaa ccagtccgaa cgcgtagtgc gataacccaa tttcaccttg ctatcatcag
     4681 cgttgaagcg tttgatcttt gcatcaatta cccgccattg aagcgaccaa attcaaccgg
     4741 cacaaaactg tcataaagcg tcacattgtc cagtaagctg acatcaagat aatacccctg
     4801 tgacatcccg ctaatattgg ttgcactact ggcatcggac tcattcgctg ggttcaggtt
     4861 attagttgca ctaataccgt caatcaaata ggcattctgg tagggcgacg caccgtgaat
     4921 agagattttc tcagggcgaa tatctccctg gttcaacgag gtactttgcg ttgaatccat
     4981 gcgtaccgca gggttggttc tcagcagatc gctgatattg ccattcccgg tcggtaaacg
     5041 ctcgatgctt tcactggtgt aatgcgtgtt gccgtttacg ggtacaggga caggagcata
     5101 gaccgtcatc tcttcaaaaa aatgcatgta catgtttttg tcatcgacgg cctgcgccac
     5161 atcagcgcca cataaaatga cgccaggaat aagaactcgc ttcatttatt tatatctccg
     5221 tacagcaggg cttttattgt tgttataaaa ccgtgctaat gtcacaaata tcatcagcca
     5281 ggttccagac accgggttga tgtgtaacca taatgacgcc gcttgtgggt agtttttcac
     5341 gcactaaacg cagtaagcgt atagcctctt gttcctcaag atgagaggta gtttcgtcaa
     5401 gaaatatcca tttcggacgt cgtaaaatta atcgtgccag ggcgatacgt tgtttttcgc
     5461 cgctggaaag aatatctccc cagcgatcgt ggtcatgaat acgcgcagcc aatttcccca
     5521 gaccaacctg atgcagtact tcgctcaacg atttatcgtc tacgggcagg ggaagtgctt
     5581 tacaaataat ctctttcagt aagccgcttt tgattaacgg tgtttgtgac acataccaac
     5641 tgtcagcagg agaagaaata tcacctttaa accacggcca gcagtgggat aatgttttaa
     5701 gcagtgtggt ttttcccgca ccagagtagc ctttcagtaa tagccatttg cctggcgaaa
     5761 catgaaagtt caagttctct aatatgatct tattatcagg cgtacgaata ctcgcattag
     5821 ccacttgtac cgcatgttgg caatttttag gcttattcgt agggcgctgt tcagtgagtt
     5881 gatggaactc atacaagcga tcgataaccg cagccagttc agcaagttcg tcatatttat
     5941 aaataaacca gcttaaattg ttcgatacta gcataaatgc ctggcgcgat ttcatcagtc
     6001 cgcccagatt gatctgcccg ctaataaact gcggcaataa cagaaagtag ggaagaacgc
     6061 taagcgagcg cgaataaata ttttgccagt aatcaagcca ccgctgacga ttcattaaac
     6121 gatgccagtt ctctttaatg gtatgaaaat tgtcgcgcaa ctcctggcgt tgtagagact
     6181 cagcattgct tagcgcaatc agttctgtct gcttattatg ctgcacaaga ttagttcgaa
     6241 atgtcgcttc gctacgttgt ttttccacat taagcggacg aatacgttta cccaccttat
     6301 gggtaaataa agttccaccg atcacaatga gcacgacggt atagaccata tacccctgga
     6361 tattccattc tgttccacca acagtgaatg agagcgtacc cgcgctttgc caaagaataa
     6421 cggtaaaggt gatcagcatg ctaagtgact ggatgaagcc aaatgaaaga ctgagcgttt
     6481 tgctgatcag taagagaatg tcttcagcga tacgttggtc agggttatct gtatttttat
     6541 gctcgccgta gatctgcgtg aagtagtagt ttttatctgc aaaccagcga ttaaggtagt
     6601 aatctgttaa ccattcgcgc cagcgaatag tcagtagttt aattaaccat gttttgttta
     6661 cggatattaa tacaaagatc cccagcaggg cagggaacca aagaacaagc tgccaaagct
     6721 tatcggtttc tttctggctt agcgcattga aaaaatcatt attccagtcg tttaaccaga
     6781 cctgaatttt aaccacgcca aggatcattg cgaggataat aatgattaac aacactgaag
     6841 ttttattatt cttacgcagc caaaagggct tcagcagaca gagatacttt gctatcaaca
     6901 tataaaacgt aatgggaatg gttatcatta gcgaaaattg attgtgcc
//
LOCUS       DAZRUF010000215         6940 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZRUF010000215 DAZRUF010000000
VERSION     DAZRUF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.216"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            6..191
                     /gene="ymgJ"
                     /locus_tag="ACHXOL_003493"
     CDS             6..191
                     /gene="ymgJ"
                     /locus_tag="ACHXOL_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR6810384.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            152..271
                     /locus_tag="ACHXOL_003494"
     CDS             152..271
                     /locus_tag="ACHXOL_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR6810385.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(750..1016)
                     /gene="minE"
                     /locus_tag="ACHXOL_003495"
     CDS             complement(750..1016)
                     /gene="minE"
                     /locus_tag="ACHXOL_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR6810386.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1020..1832)
                     /gene="minD"
                     /locus_tag="ACHXOL_003496"
     CDS             complement(1020..1832)
                     /gene="minD"
                     /locus_tag="ACHXOL_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR6810387.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1856..2551)
                     /gene="minC"
                     /locus_tag="ACHXOL_003497"
     CDS             complement(1856..2551)
                     /gene="minC"
                     /locus_tag="ACHXOL_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR6810388.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3071..3439
                     /locus_tag="ACHXOL_003498"
     CDS             3071..3439
                     /locus_tag="ACHXOL_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR6810389.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3547..3948)
                     /gene="pliG"
                     /locus_tag="ACHXOL_003499"
     CDS             complement(3547..3948)
                     /gene="pliG"
                     /locus_tag="ACHXOL_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR6810390.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4190..4483
                     /locus_tag="ACHXOL_003500"
     CDS             4190..4483
                     /locus_tag="ACHXOL_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR6810391.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4555..5214
                     /locus_tag="ACHXOL_003501"
     CDS             4555..5214
                     /locus_tag="ACHXOL_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR6810392.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5306..5752
                     /locus_tag="ACHXOL_003502"
     CDS             5306..5752
                     /locus_tag="ACHXOL_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR6810393.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(5959..6870)
                     /gene="hlyE"
                     /locus_tag="ACHXOL_003503"
     CDS             complement(5959..6870)
                     /gene="hlyE"
                     /locus_tag="ACHXOL_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR6810394.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1789 a         1541 c         1611 g         1999 t
ORIGIN      
        1 tttatgtgag cagaagatat tcatcagcaa cgattacatt agtcatttta ttttgccgac
       61 ggcctcattg tcgaaagata agcgtacgac agtattatct gaaaagagtg attttttatc
      121 caactacact tcagcgcact gcgtttaaaa aatgcctctt tcttatgcgg gatatcatca
      181 tttcatcatg atgtctttgg tgagcggtga acacaataca cctgcgctgt ctcttcagga
      241 tgaatcccct ggtctggtgc ctgcgggctg atgttgcagc agagcttagg tcacttagac
      301 gctactatca tttatccaat ggcatggaat cgaaatcagt cgatacccgc agtatatatc
      361 gtgaactggt cgttctgacc atcaacgatt aaactgcgct tcggcgcagt tttcgtttac
      421 agggggctaa aagggaaaat tctggggcaa aaaaagcccg ccagttacgg cgggaaacct
      481 catcctatgg gagaacaatg aataatgaaa ttgcggggtt atcatccccc agtgtatcca
      541 cactaacaat aaggttattt actcagccag gcataaacat tatgttctgt gcgtgggaac
      601 agccttaagg tgtaaagggg gaggtggaaa tagcaatgag gagtatcagc aagaatactc
      661 gccgctttac cacaacgtgg atgagaggga tgaaaaactc aaggcagaaa tgactctgcc
      721 ttgaagataa atgcgttttt acagcgggct tatttcagct cttctgcttc cggtaaggtc
      781 acgttcagct caagaataga aatatcgcca tctttttgct caagctgtac ggttaccatc
      841 tcaggatcaa tttgtacgta tttacaaatg acctcaagaa tatctttacg caactgcggc
      901 agataatgcg gttctgcatc gctgcgacgg cgttcagcaa caataatctg cagccgttct
      961 tttgcaatgt tggctgtgtt tttcttccgc gagagaaaga aatcgagtaa tgccataact
     1021 tatcctccga acaagcgttt gaggaagcct ttcttctctt cttcaatgaa gcggaaagga
     1081 cgttcttctc ccaacagacg ttctacggta tctgcgtagg ctttacccgc atcggcgtta
     1141 atgtcgagaa tgaccggttc accttggtta gaggcgcgca atactgattg atcctctggg
     1201 atcacgccga cgagtttgat gcgcaggatc tccagcacat cttccatgct cagcatgtca
     1261 cctctgctta cgcggcctgg gttatagcgc gttaacagca ggtgctcttt aataggctct
     1321 tcgccatttt ctgcgcggcg tgatttcgac gccagaatgc ctaaaatacg gtcagagtcg
     1381 cgtactgagg agacttccgg gttggtggta ataatggctt cgtctgcaaa atagagtgcc
     1441 attaacgcac cggtttcaat ccctgccggg gagtcacaaa cgataaattc aaaatccatc
     1501 gctttcagat catcaagaac tttggcgacc ccttcacggg tgagggcatc tttatcgcgt
     1561 gtttgcgatg ccggaagaat atagagattt tcagtacgct tatctttaat taacgcctga
     1621 tttagcgttg catcgccctg aatgacgttg acgaaatcgt aaacgacccg gcgttcgcaa
     1681 cccataatca ggtcgagatt acgcaggccg atatcaaaat ctatcacgac agttttcttt
     1741 cccttctggg ccaaaccagt ggcgatggcc gcgctggagg ttgtcttacc aaccccccct
     1801 ttgcccgaag taacaacaat aatgcgtgcc atagaaattc cttgttaaaa agggatcaat
     1861 ttaacggttg aacggtcaaa gcgttttcga ctaactgcag tcgcgccgct ttgccataaa
     1921 attctgctgg gatttgatca ctcagccagt attcacctgc gatggacacc aattccgcca
     1981 tcaggttcgt acaaaatatt tgcgtttccc ggtcaccact tgcccctgcc agcgcacgac
     2041 cgcgcatcat gccatagaca tgaatgttcc catcggcaat caattcggcc ccagcgctaa
     2101 cgtggcttgt aacaatcaga tcacattgtg gagcataaat acgctgaccg gaacgcaccg
     2161 gggtatctat taaacgcgtt tttgtgaccg gcgtggtatt ttgcgctgga gcctgtggtg
     2221 cgggagctgg acgtggcgct ttttcctttc cttccgtcag gataggcagc cccatttttt
     2281 caatttcggc tttaagttgc gcgtctttgc agccacttac gccaataacc cgcaaaccgg
     2341 ttgccgaaac cgccttatgc atcgctgacc agtttaccgg gtcttccagt gcactgacgt
     2401 tgagtacaac gggggcatgt tttaaaaatg cgggggcctg agcgattttg tcttccagcg
     2461 cctgatggat aaccttaggt tctgcctcat gcagatgaac cacagataaa gtgaagctac
     2521 tgcctttaag ctcgattggc gtgtttgaca tcctggcctt actcaattag ctattaatca
     2581 tcgccagcgc gcgatgatgt tccgaagact ataaggcatg ttatagtctg gattatattg
     2641 aggcaagtca ccctcccatt tattcagagt aaaagtctat tctgtgataa atggcgctga
     2701 ttcatagctt aaaaaatacc cttgtcaatc aacccattgc cgccgtactt ttgattcttc
     2761 ttatttacgc ttcctttttc cgcaccctaa tcgtgacagc atctccagac ctgtttaaat
     2821 atccagcatt gaggcaagtg tattttttca ccacgccagg ctttcttaac tttccatttt
     2881 ttgcagcatc attaaagaac agatacccgt tatcttaata tccgccacag aattgctcta
     2941 tcatagaaac cgcagaagac gtctgatact tctgtcaggt catcgtaact cgtccaggtc
     3001 agcggttttg attacggatt gatgacgaaa tgagcataag tgttgtacgg ttggattatc
     3061 aggatgtgct atgaatatga tgcgtattat ttatatcgga ttgtcaggtg tggggatgat
     3121 gttctcatca atggcgtctg gacatgacgc tggcggactt caatctccgg cgtgcggcgt
     3181 tgtttgcgac ccgtatattt gtgtgaactc agatggcatt tccccagagt taacaaggaa
     3241 atatctcggc gaaaaagccg ctgaaaactt acaatcatta caaggctacg atcccagcga
     3301 atttacattc gctaacggtg tattttgcga tgttaaagaa aaattatgtc gtgatgatcg
     3361 ttattttggt gtagatggta agcggagtgg aaaaatcaat caaaccacca caaagatgtt
     3421 atttatgtgt cgtgagtgat gccttgttta cattatgttc tctgctattc gtcattaacc
     3481 tcttgagcga cagagaggag tggggcgtta acgtctatga tggggatagt ccccgtcggt
     3541 tgacatttac ttaatttgaa tatcgacgtt atatttttta gttttgtttt tacgggcgtc
     3601 gtttcgagtt tgcagcactc gcagctcata tttgccagaa gcagggagtg agtattgtcc
     3661 gtgactatcg agttctgggg aatatctgga taaatcaaca gaatcatcga ttcctggccc
     3721 gaagagataa gtatcagcgc cttcattaga gatactcaca tgcactttct gacctttttt
     3781 ggcatagaag gtatatgtat cgtaatcgta tccctttatt tcgccggagt actgagcgct
     3841 gctatggcct ttgctgaact caacattgac atttttaccc gcagcaaagc tcgtggaggt
     3901 tagcagtgca agcagcaata cagccttact gatgctcttg attttcattg gtagtctcct
     3961 gttcagaatg agtacgatga actgttataa tataacaatc ccttacggga gaattccagt
     4021 ggcttttctg cgttcggtaa aatcccggcg cggcattttt tgccgggggg attacttata
     4081 aaggggcatg gactcaacaa accgcagaaa tctacagctg gttggcttgt ggcgcgggtt
     4141 tcattgatgt tagcttatgc caaaacctgg catcttaaag agtaaaagta tgttttgtgt
     4201 gatttatcga agcagcaagc gtgaccagac ctatttatat gtcgaaaaaa aagacgattt
     4261 ttcgcgtgtt cctgaggaac tgatgaaagg ttttggtcag cctcagttag cgatgattct
     4321 gccgttggat gggcggaaga aactggtcaa tgctgatatt gaaaaggtta aactggcatt
     4381 aaccgagcaa ggttactatt tgcagttacc gccaccacct gaagatttgc tgaagcaaca
     4441 tctttccgtc atggggcaga aaacagacga cactaacaaa taaccgatat ccggcggtgg
     4501 cattatcttt gtcggcgcgg gttttcatat ccacgataag gtgaggggaa cgttatgtat
     4561 caacatcaca actggcaagg tgcgctgctg gattatccgg tgagtaaagt agtctgtgtt
     4621 ggcagtaact atgccaaaca tattaaagag atgggcagcg cagtgcccga agagccagtg
     4681 ctgtttatta aaccagaaac ggcactgtgc gatctgcggc agccattggc gatcccatcc
     4741 gatttcggtt cagttcatca tgaagtcgaa ctggcggtgt tgattggcgc gacgctgcgt
     4801 caggcaacgg aagagcatgt ccgcaaagcc attgccggtt acggcgtggc gcttgatctg
     4861 acattgcgtg atgttcaggg aaaaatgaag aaagccgggc agccgtggga aaaggctaaa
     4921 gcgtttgata actcttgtcc gctttccggg tttatccccg cggcggaatt taccggcgat
     4981 ccacaaaata caacgctggg cctgagcgta aacggcgaac aacgccagca aggtacgact
     5041 gcggacatga tccataaaat cgttccgttg atcgcttata tgagcaagtt ttttaccctc
     5101 aaggccggtg acgttgtgtt gacaggcacg cctgatggcg tcggcccgtt gcaaagcggt
     5161 gatgagctga cagtcacttt cgatgggcat tctttgacaa ctcgcgtttt gtaatacttt
     5221 ttgccgcctg aaagcggcgg caaaacttgc atcgctgtgc cagactggtt ataaggtgcg
     5281 ttttaacgta atggcggaac acctgatgag cgatgtacct ttctggcaaa gtaaaaccct
     5341 ggacgaaatg agcgatgcgg aatgggagtc gttgtgtgat ggttgcggtc agtgttgcct
     5401 gcataaactg atggatgaag acaccgacga aatctacttc actaacgtcg cctgtcgcca
     5461 gctcaatatt aaaacttgtc aatgtcggaa ctacgaacgt cgtttcgagt ttgaacccga
     5521 ctgcattaaa ttaacccgtg aaaatctgcc aacattcgaa tggctgccaa tgatctgcgc
     5581 ttatcgtttg ctggcggaag gtaaagattt acctgcgtgg catccgctac ttactggttc
     5641 gaaagcggca atgcatggtg aacgtatctc tgtgcgtcat atcgcagtga aagaatcaga
     5701 agtgattgac tggcaggatc atatcttaaa taaacctgac tgggcgcagt gataaattta
     5761 atagatgttg atgggggctt tcttaatata cgtctggatg ctcgccattt cgtagccctg
     5821 tttttgaaag cgctctgtca taatgagagt tacccgatac cttattatca acaggtaact
     5881 ctcaatcttt aaaaaacgtc tgtgttttta aatgtgataa accttatacc ttgagtacat
     5941 ggagagaata atcgctgatc agacttcagg tacctcaaag agtgtctttt taccgtgttt
     6001 tttctgatac tcattacagg tgttaatcat ttttttggcc gcttctttta gcaaagaaag
     6061 cattaaatca tcataatcaa cgtagaatct ggtggtttca gtttccgttt ttatctctcc
     6121 gatggcggct atttcggtgg ttaatttcaa tttggcggca tcgatatctt tattcgcttg
     6181 tttaaccgtg ttagacaggg tggtaaagaa actctgcaca gattttaact tgttcttcaa
     6241 ttctgggatc agtttccctt caactacgcc cgcagcaata gaataggaaa tgattaaacc
     6301 aaatggaccg gcgacgacac cggctgcggc accggcatac gcttccttcc tgattttatc
     6361 tacctgtgac tggaaatagc tgcttttttc tgaaaaatca ttggttaact ggctatctaa
     6421 cgccagcagt ttcccggaag cgttgttgaa actttgtgag cttaccagca gggatttttg
     6481 cgcttcattc agcttcgtga tgccgtcatc cagtacctta atgagaatgt ctttctgggc
     6541 ggatgctttc ttctcattgt actcatcaaa tagcaaaata tacgcagcga gcaattgcgt
     6601 cgcaacacca caccattcat acactgtttg ggttgcttca aaatacttat cctggctatc
     6661 cataagtaag gttttaatat cgccgactaa aacggaggct gcctgtgaat actcctgttt
     6721 aaagcgactt aactctttta tggtttcatc aaaggtctgc caggggatga cctgatcgag
     6781 atatttatta taaagatcta atgctccatc tgcggtttcg attgcgtttt taactacttc
     6841 taccgtttta tctgcaacga tttcagtcat aatcattcgc ctctttaaat atataaattg
     6901 taatgaaact cctgttttac aactattaat aaattttact
//
LOCUS       DAZRUF010000216         6851 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZRUF010000216 DAZRUF010000000
VERSION     DAZRUF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.217"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHXOL_003504"
     CDS             complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHXOL_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR6810395.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
     gene            complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHXOL_003505"
     CDS             complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHXOL_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR6810396.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHXOL_003506"
     CDS             complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHXOL_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR6810397.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHXOL_003507"
                     /gene_synonym="cse2"
     CDS             complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHXOL_003507"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR6810398.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2496..4058)
                     /gene="casA"
                     /locus_tag="ACHXOL_003508"
                     /gene_synonym="cse1"
     CDS             complement(2496..4058)
                     /gene="casA"
                     /locus_tag="ACHXOL_003508"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFR6810399.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFI
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRISELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVLLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(4156..>6851)
                     /gene="cas3"
                     /locus_tag="ACHXOL_003509"
     CDS             complement(4156..>6851)
                     /gene="cas3"
                     /locus_tag="ACHXOL_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFR6810400.1"
                     /translation="KYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVA
                     AMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPEL
                     KIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGH
                     HGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYI
                     SATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGINT
                     LFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGL
                     FFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGS
                     EEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKIL
                     LADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQE
                     APFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGK
                     CIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDS
                     SQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGK
                     DERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPH
                     AARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLP
                     EKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEG
                     EAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1659 a         1862 c         1623 g         1707 t
ORIGIN      
        1 ggagttcttc ccgacgataa agaaattgcc tttctttgcc gccagggaaa agatcccaca
       61 gccactgatg catcacatat tcaccgcgat ccaccagatg cagcaactgc gcaggcgaaa
      121 gctggccggt atgtaaggtt attcttgaga ggtacatggt tcctccttgc tgagccacgg
      181 cccctgattg acggtgcgtt ccccaaacag ccactgctga cgatttaaag gaacatcccg
      241 gcggcgtaat attttgctcg cgaccaggcc gtcgtgtttc ccttcccacc agcattcatc
      301 ctgaagtttc gggagtgaga ctttcagttt atggaaatga tcctgatact gttggtatgc
      361 attacataag gcatcacacg cgttgccttc gagcagtaac ggcgcaagag gtaatgccag
      421 cggatgactt tttcgcccca gataaagcgg aaaaactgga tggcgtaaac cgtcctgcaa
      481 ctgttcgagg ctgtaaggcg catcggctgt tgttgccacc gccaccatcc accaggcatc
      541 ggtgtagtag tcgcgccggg agataatcgc gctcaaaagc tcagggtcgc tcaactcttc
      601 acgacggctg aaataacgtg ctttacgcac ctcttttggc atctggaccg tgtgataatc
      661 ccgtgcccag cgcgggttac ggctggcgca aaccaccagt gaatagtggc gattaaacgc
      721 gtttaagcgt tcggtatcat cacgccgaat ccctaccccg gcagccagta gccccagcaa
      781 tgctgagcgc gaaggaagtt catgggtatg acgaacttca cctggagcat cgacacccca
      841 ggacgccatc ggcccatgaa gctgaaaaat cagatattgg ctcataagcc gccccttacg
      901 cgcagataaa gtccagcacg tccttcatgc ttccctgttt gttcatcacg tcaaaacttg
      961 cgcattcggt cttctgttcg tagaccgtat tcatgttttc gcgaagcgtt gtaatacgct
     1021 gcaccgccac atctaactga cgtgtgccat taatgggttc atagaaagcc gccgccagag
     1081 agcgtggttg atcggtgcct ttttctgcca gcgcccagga agcataggca cggctggcaa
     1141 agctattttg tttaccggtt ggggaaactt taagcgcggc ttccgtaaaa gcgcgtatgg
     1201 tctgattagc taacgcttcg tcaccgccga ggttttctac cagcagatct ttatcaatgc
     1261 agatataggt gtagaaaagt gctgaaccga atccggtttc tccgagatgc ccggcaccgg
     1321 catcttcaga ggcctggcgc aaatcatcaa cggcggtgaa aaaatcatct tcgacaatcg
     1381 tttcactgac accaaacgca tgcgcgacct ggcaggcggc ttcaacatta aactcaggtt
     1441 tattcgccag catacgacca aacatagcga tatctaccgc tatacggtct ttacgtaaca
     1501 acgcgagatc ttcctctttt ggcgcgcgct tttcttctgc cagttgatga gcaagcgctt
     1561 ttacggcgtc aaattctgcc gggctgatat gaactaattg ttcagtttcg gcgttagtga
     1621 gcggatcttt tggttttttg tcatttttag ctttcccaag ataatcagca atttttgccg
     1681 cccattcgat ggctttttta tcttcaatcc ctttctcaat caggatagtt gccgcctcac
     1741 gcgcaatacg cccactgcga ataccaatat ggcccgccag tgcctgttca aaaagtgcag
     1801 aagtgcgcca cgcacgtttc agactttgcg aggaaacgcg cagacgcgtt gctccaccca
     1861 gcaccacagt ttttggcgca ccggtgtcat cacggttaag gttggcagca gggtaagcgg
     1921 ttaacacatg aagctgaata aatgttgtca tgagatagtc ctttattgat tttgttcgtt
     1981 atcggcgtcg ccagcctggt aatattccag cgcccagcgg atacggataa attcggttgg
     2041 ccgctgctgg cggcggtgat gattgagcag gtcgtcgctc tcctggcacc agcggaaaat
     2101 gccatctgcc agggagtcaa gattgacgga accgtttaat aatctgacgg cgcggcgtaa
     2161 ctggcgcagt aattcatccg gtgtttttac tgctgacagg cgagtaaaac gcccctttga
     2221 cattaccgcc gccagttgcg cggcaaaagg ctgccgttca tcaatggctt taacattggc
     2281 gctaactgcg gctatcagcg ccagtgcggt gatacgccac tcaggttcat cctgccactt
     2341 tatttgtctg ttcttcagaa acaggcgaaa tccatccgtc agacaaacat cattaaccgt
     2401 cgtactacgc cgcagactgg cacgttcgcc gcgtttctcc tgcaattcct cgtgccattt
     2461 gcgcagcgtg gctttgtgct cctcttttac aatactcatt cagcagcctc ctgctttttc
     2521 tccctggcgg ctttagcact ttgcttctcc gccgatgttg taaaatattt cttgcgcgcg
     2581 gtcatgatgc gttccaaatc aacgggctca taaggattgg tgaatacacg ctcgtcaaaa
     2641 tcctgacgcg cgaataacca aatttcctta ttccatttgc tgagtaattc atccgcatcc
     2701 tgaccttctt caatttggcg cactaacctc aggaagcgat gctgagtttt gttccagaag
     2761 tcgatatcca caaaactaaa atcacccctt gcaccttttg gatcggagaa ccatgcttct
     2821 ttcaatgcac tccgtaacag acttagaatc cgtgaagccg tttgcgcagc cagccgcagt
     2881 ttcggtatct ggccttcttt tttattgagc agcagcggga aatgatgttc gtaccaacag
     2941 cgcgctttca tgttgtcgaa atcatagcca aatccccaca ggcccacttt tgcctgcttg
     3001 agactgctgg cattaaagag tttcaccacc agcgcgggaa gttccgtatt gttttctgac
     3061 ttacccgttt cgataaggcc taaccagtcg cgccagatta aaccgcccgg ttgtggtttg
     3121 accgagtaaa actcgccgcc ctctttaagt agtacacggt aaggcgttaa gggatgctgc
     3181 cacatggcat agttagcgcc gtaatttttg gtcgtcatca ggctcagaag cgcgtcgctc
     3241 tgctcaccgc aaatatcgca gttgccgact gtggtggtat taaaatcaat acgaatacgg
     3301 cgaggcattc cccagtacgc ctggagttta ttgacctgct catcggttac caccgcaccg
     3361 gccagttcgc tgatgcgcgt cgggccaagc caggggaaaa ccagatcgtc aaattttttg
     3421 ggtagcggta aatcggcttc atcctgcggc atgacattga tccacagttt gcgccacaag
     3481 ggagtttgtt gattaccctg atattcctgt aattcaatca gagtggtcat cggcccaccg
     3541 ccgcgtaaac cggtgcggta gcctttgcca cctgacggcg cattcaactg tagggagaac
     3601 agagctaacg cagagcaatg tgggcacagg tattccgtta caccacgctt gataaagtgg
     3661 tctttattaa acttcgttgt ttgagcgccg ggaatctcag gcagtagcga agcgaccgga
     3721 actttatctc cggtgagcgc ctcgaaatcc tgtataaatg aaggggaatc tggcccaaac
     3781 tggaaagcat gttctaatga cagcaatgct tcccgtagct tttcagcttc cagtccgtct
     3841 tcccagatat catcccaacg acgatgatct tttggcgcga agctggtttg tagtagcccc
     3901 agcaaaaact gccataccgc accctggaga tctgcccgcg gcgcagaaat atcgacaaca
     3961 ttttcatccg ccagatcgac tggcgccagt ttgcctgtcg ttccgtcttt aaaacgaacg
     4021 ggcaaccacg gggttgtcag aagtgaaaac gagttcatac gcatcctctc gtaaaaaaga
     4081 ccatcccagg tcggttgtcc atctttccat ccttttcggt gtatcaacta ttcagagagt
     4141 cctgtcgcag acatctcaac caaccttgcc aatcaatcct tccgtagccg aatacccgca
     4201 actttcgtca tcgttgacta aaataacgtt tgccatctcc ggatcttgtc gctgttcaat
     4261 gcaccacagc ctgaacgcct ctccctccag taaagaaaac tcatcccgat gttttttcca
     4321 ccagcttcgg cgcactctga caacgctcat ttcccatgcg tgagcaccgg tggcataagg
     4381 cttcaccaca ccgtcaatac aggtagccag ccacagggaa acagactcct cagccagacg
     4441 tgtcgacagt ttttctggga ggtaatcgtt gatattggcg gcatagccgg gcctgaagtt
     4501 cagcacaaac tttttagcca ttgcgcgatc acagtaatat ttgcccactt gctcctgctc
     4561 gctgcgggca aatccttccg gcattaccac gtcctcaccg tagacggatt caataagaag
     4621 ccgggctgcg tgtggcatct gaatagcacc ttgctcacgc agcacacgct gcgtcagcca
     4681 gattcgccca tgatcgggat agacaaatgc actgttacgc atggcactgc caaaccattc
     4741 gtcgccagga gagtcgtccc agacgggagc cagaatcagc aattcagggg gggaacgctc
     4801 gtcctttccg tcacgcttta actgaccatt aatatcgcgg atatgccgct gtaatcgtcc
     4861 tgctcgctga atcagcaaat caacaggggc caggtcggag atcatttcgt ccaggtcaca
     4921 atcgacactc tgctctaata cctgagtaca aataaggact tttccggcac gctgtgagct
     4981 atcttcttta ccaaagcgtg ccagtgtctc cgtttcaatt cgctggcgat cgctaaaagc
     5041 aaagcggcta tgaaaaagtg aaaggctgga agcaggaatg acgccgcggg caagcagctg
     5101 acgatgaacc ttaatagcgt catcgacaga attccggatc caggcgatgc atttcccctg
     5161 acttaccgcc gattcgatac gcgcaataca ctcttgttca ctatgaagcc aacccacgct
     5221 gacgctacgc tcaacgtctt tgcgcgtcgc tacccggtgt gagttcacat cggatttcgt
     5281 gacatgcgtc agccaggggt aatcatcctt ttccaggaac ggagcttctt gcaggccctc
     5341 tgtaccccgc gcaaaggcag cgacgagttt gtcgcgctgc tgttgagata acgtggcaga
     5401 aagcaaaata acgctgtttc cgccacgcgc ctgccgctcg atcaaccctt caagaatgca
     5461 cgacatgtaa gcgtcacagg cgtggatctc atcagccagc aggattttgt tactcagccc
     5521 cagaagccgc agattattat gtttgaacgg catcaccgcc atcatcgcct gatccagcgt
     5581 gccgacgcca atttcagcca gcaacgcctt cttgttactg tcggcaaacc aggccgcaca
     5641 tccctgactg aacgtttgtt catccggttc ttctgacccg actaaatcac cagaccagag
     5701 tgattcatta aagcggtcca ttaatgtgcg ggcactatgt gccagcacca ggctggggcg
     5761 ggattctggc gaatagaaag caagccaggt tttgaccagc cgatcgtaca tggcattggc
     5821 cgttgccatt gttggcagac caaaaaacag cccctgagcc ttccccgcag ctatcaacct
     5881 gtgcgccagg ataagcgccg cttctgtttt acctgcgcca gtcacatctt ccagaataaa
     5941 taactgcggt ccttgctggc tgatatccag atccagcacc ttttgctgta acggtgtcgg
     6001 attctcaata aaaggaaaca gcgtattaat tccgttgaaa ggcgcgactt ttgcttttag
     6061 aggaaagacg gttaaggcgt tttgagcctg aactaaagtt ttctgccagt aacctttaat
     6121 atccattggg tgtgcgacgc gtggaaaaaa tcgcgttgac gaacccgtcc agtctgcgag
     6181 cacgacggtt gcagagatat accaggaaag gtgttttata agttcaatgc cctcatcatc
     6241 atcccagaat gcggggatct ctatgagcgg aaacagtggt ttgatttcaa ggagaaaatc
     6301 tcgtgctgca gctttgtctt caggcagaaa attatccaat tcatccatac gatcaggggg
     6361 ccgaccatga tgccctgtcg tcacgggcat ccacatttct atcactcgcc taagtttgcg
     6421 cggagagagc gaagatgaag gaagtaactc ctgacattca cttaaataat gattccacaa
     6481 ccagtagccc agcgttgaat gagagatttt ttcgtaattt tttctggcac cttcctgaat
     6541 cttgagttca gggggcaggt aaagttgttg aaaagagcgg gcaaattttc caatatcgtg
     6601 ccagcacagc aaccaggcga aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg
     6661 acgaaaataa tcagccagcc cgaagcaatt tcttttaacc attaaatagc ccatcgcggc
     6721 tacatccagc gaatgccagc aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg
     6781 tcgggttttt ccccagaaat caaaaaaagt cacaatattt ttatccttca gtaaacttaa
     6841 aggatattta c
//
LOCUS       DAZRUF010000217         6827 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZRUF010000217 DAZRUF010000000
VERSION     DAZRUF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.218"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(155..667)
                     /locus_tag="ACHXOL_003510"
     CDS             complement(155..667)
                     /locus_tag="ACHXOL_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR6810401.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            862..1626
                     /locus_tag="ACHXOL_003511"
     CDS             862..1626
                     /locus_tag="ACHXOL_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810402.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1577..2980
                     /locus_tag="ACHXOL_003512"
     CDS             1577..2980
                     /locus_tag="ACHXOL_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR6810403.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            2980..4446
                     /locus_tag="ACHXOL_003513"
                     /pseudo
     CDS             2980..4446
                     /locus_tag="ACHXOL_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4337..5080
                     /locus_tag="ACHXOL_003514"
     CDS             4337..5080
                     /locus_tag="ACHXOL_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR6810404.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5084..6304
                     /locus_tag="ACHXOL_003515"
     CDS             5084..6304
                     /locus_tag="ACHXOL_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR6810405.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6308..6814
                     /locus_tag="ACHXOL_003516"
     CDS             6308..6814
                     /locus_tag="ACHXOL_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810406.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1816 a         1622 c         1868 g         1521 t
ORIGIN      
        1 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
       61 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      121 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      181 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      241 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatataa cgcagcacgg
      301 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      361 gttctattgt attggaacgg cttgatatta tacatgcaag ttgagggata tgcccaatta
      421 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      481 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      541 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      601 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      661 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      721 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      781 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca gcgataacag
      841 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
      901 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
      961 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1021 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1081 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1141 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1201 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1261 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt
     1321 cggtggttgg tgtcgctgga agatgctaat ggggaccagg aaaggaagat gctgattgaa
     1381 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1441 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1501 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1561 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1621 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1681 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1741 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1801 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1861 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     1921 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     1981 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2041 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2101 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2161 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2221 ggagtctgat gatgatgcgg caatcatcaa actgtcttgg atagaagccg cagtggatgc
     2281 gcacaaaacg ttaaatttcg aacccagtgg aagaaagcgt attggctttg acgtggctga
     2341 cagtggtaca gataagtgcg ctaacgttta ccgtcacgga tccgttgttt tctgggccga
     2401 cgaatggaag gccaaagaag atgaattact gaagagctgc cagcgtactt atcaggcggc
     2461 gctggagcgt gaagcagata ttgtttacga ctctatcggt gttggtgcgt ctgccggtgc
     2521 taaattctct gaaattaacg ctgaccggaa gagcgagaac gcatacgcgc gacgtgtgaa
     2581 ttaccagagg tttaacgccg gtgctggtgt gcatgagcca gatgacgagt acaacggcat
     2641 ccccaacaaa gactttttcg caaatcttaa ggctcaggca tggtggctgg tggctgaccg
     2701 tttcagaaat acgtttaacg ccattaacaa cggagaacag tatcctgtgg atgagctgat
     2761 cagcatagat tctcgttgtc cgttgcttga aaagctgaaa ctggaactga caacacctca
     2821 tcgtgatttc gaccgtaacg gacgtgtgat ggtcgaaagt aaaaaagacc tcgcaaaacg
     2881 cgagataccg tcaccaaacg ttgctgatgc attcattatg gccttcgcgc caatcgatac
     2941 atcgctggat atctgggaac agctggggag acaggcctga tggcacgaaa caaacaagcc
     3001 ctgcggcgaa ctgtgcaggc cacagctgat ggttatgaga attttattgc ccgcgtaggg
     3061 atgcagacac ctaaccagca ctcagcatcc acctaccggg ctaatttcac cagtcgtaac
     3121 cgcatgctgg tggaatggtc ctatcgttca tcctagatca tcggcgaagc agtcgatgct
     3181 atcccggatg atatgacccg caaaggcatt cgcatcactt cggaaattga tgcaaaagat
     3241 cgtggcattc tcgaatcaca actggatgag ttgcaaatct gggatgcgct gaatgacgtg
     3301 ctgaaatggt cgcgcctcta cggcggcgcg gtgggtttca tcatgattga ggggtaggca
     3361 ccaatgaccc cgctgcgacc cgaaaccatc ggtaagggca agtttaaggg gattctcccg
     3421 ctcgaccgct ggatggtcga cccggcactg acccgccgca ttaaagatat ggggccggac
     3481 ctgggtaaac ctgagtttta cgatgtggtg accacagcaa cgggaattcc tgcctggcgc
     3541 attcatcaca gtcgcctgat tcgctttgat ggcgtcacgt tgccatttca gcagaagatg
     3601 accgagaacg aatggggaat gtcggttgta gagcgtatct gggatcgtct taccgcgttc
     3661 gacagcgcta ctgtcggcgc ggcgcagctg gtctacaagg cgcatttgcg tacctacagc
     3721 gtggagaagc tacgcgagct tatcgcactt ggtggtcctg cgtatgaagc gttgctgaag
     3781 aatatcgacc tgattcgaca gttccagagt aatgaaggca tgacgctcat ggactcgcgg
     3841 gataagtttg aaacccatca gtacagcttc agtggtctgg atgacatcct ttcgcagttt
     3901 gtagaacaga ttagtggcgc tgttggtatc ccactggtgc ggttgttcgg acagtccccg
     3961 aaaggatttt ctaccggtga tgcagacctt gccaactatt acgatcgcat cagttcgttg
     4021 caggagaggc gtttacgtct tccggtgcgg cggatactgg acatcatgca tcgttcggaa
     4081 cttggcaagc cgctgccgga cgatttcacg tttgagttta acccgctctg gcaaatgtct
     4141 gatgtcgatc gctcaacggt ggcgttaaac accaccaacg caatcagtac ggcgctgggt
     4201 gatggtctga tgacactgaa agccgctatg actgatttgc gagaaaattc tgacgtaacc
     4261 ggcatcgggg catccattac cgacgaggac atcgagaatg ccgaagatga agcgccgccc
     4321 ggcatcggcg aacctgatga cgaaccgcag gagccgtcag gcggaaatcc ggtatcgaac
     4381 cagcctacgc aggatagcga gggcggtcgg agacatcgta aatggtcgct acgatggttc
     4441 aaatgacagt atcacggaaa ttattgcggc gctggagcgt tacagtgaaa tcatcacccc
     4501 ctgggcgaca aaggtcgcgg aaaactttac tgcggaccta acccggcaga acgagaaagt
     4561 ttggcggcaa cacagcaaga acatcagtcg cgagctccgc aatcttgtgg aaagcgctcc
     4621 tgtgggccag gtgatgcaat ccatcatcgc cgaacaggtc aagtacatca aatctctgcc
     4681 tctcgaggcc gcagacaggg tgtacgacat ccagaacaaa gcgatagagg ctgttgtcac
     4741 cggtggtcgg gcggagcagt ttgctaaaga gattgcatct accggcgatg ttgctaagtc
     4801 tagggccgat ctgattgccc gaacggaact gggaagagca acgggcgcgc tggatatggc
     4861 ccgagcgata gctattggct cggatggtta tatttggcgt acagccgatg atggcgatgt
     4921 ccgagattcc cacgatcaca tgaaaggtaa attcgtccgc tgggattcac ctccaacttt
     4981 ggacggcatg accggccacg cgggcgagct gccaaactgc cgctgctata aagagatcgt
     5041 gtttgttcgc gttccattcg caatgaaaag ggcagcataa cccatgaaat acttttttga
     5101 gaccaggctc ggggaaaccc gataccgcct ggctgacggc tcgttgctgt gcaaagacgt
     5161 gccgatagga cgaacaggta agcagctcta tggtgctgat gacctgccaa aactgaaacc
     5221 cgataagttc ggtgaaatag tcgtcacgcg ttctcctgag caggtattcc atccggccac
     5281 gcttgcctca ttcgaaggga tgagcatcac gatcctgcat cctgaagatg aaaacgggaa
     5341 tgtgcggctg gtaaatcccg agaactggaa agagcttgct gtcgggcacc tccagaatgt
     5401 ccggcgcggg acgggtgagc agtctgattt gatgctggct gaccttatcg tcaaagacga
     5461 aagcgccatt cagcttatcg aagatggcct gcgcgaagtg tcgtgcggct atgacgcgga
     5521 gtacgagcag accgagccag gtaaagccga gcaggtcgat attaccggaa accatgtggc
     5581 tcttgtccct aaaggcagag ccggaaatcg ttgtgcaatt ggagacagag acacaatggc
     5641 aaatcaaaag aaaaactggt ggaaccgcat gcgtgcggcc atcaagacag gagatgccga
     5701 caccatgaac gaactggtgg agtcggctcc cgcatcggtt acaggagatg agggggattt
     5761 gccgcagggc gttaatctca acatcaacct gtccccgcag caaccactac cggacaaagc
     5821 accagagatg ggcggagatc caaccggcga cagtgatgat gacctcaaaa cattactgaa
     5881 agccctgctg gctaagctgg aaagaaatgc cacgggcgat aacgataata agcctgacga
     5941 taatccgacc ggtgacggcg aggacgatga agaggaaacc acgattactg gtgactcagc
     6001 ctggcgtgcc gaagttatcg ttccgggtat cgatctgagc cgtaagatga aaccgaccgc
     6061 gttcaaacgc gaggttctgg cttctgctga caaaacgctg gttcgccaga tagtcggtga
     6121 tgcggatatc cgcaaattac cgaaacaatc ggtcgacatg gcgtttaatg ccgtgtctga
     6181 ggttgccaaa gggcgaaaca cccgcgccac caccggcgat gcacagcgcc taaacatggg
     6241 catgaccagt atcgcttccc tgaacaaaca aaacgctgaa ttctgggcaa accgtaaagg
     6301 gtaaaaaatg aataatgtat ttctgtaccg gatgcctgtt ggcattgccg gggctgtctc
     6361 tcgcccgcag gacttaaccg tcgaaccggt ggtccttaaa tccgataacg ccttcgctgc
     6421 ctatggcctg gctggtaaat acgatgatga cggttttttc gtgccgctgg cagatggtga
     6481 taccgcagac aaggtgaagg ggatctacgt gcgcccttat ccgaccacgt cgcagccgga
     6541 catggttcgc caggtgggaa caggcaagaa cttcccgggc gacgccatga agcgtggcta
     6601 cgtgaccgtt aatctcggtt ctgattttga tgccagcact atcaaaaaag gcgatccggt
     6661 atacgttgtc gtctccactg atgaatccat caaagtgccg ctgggtggat tcatgtccac
     6721 gtcagtcagt ggcaaaaacg tggtgctgac caacgctgaa ttcacaggtg ccggtgatgc
     6781 tgacggcaat gcagaaattt cctggaagat ttaaggaaca gacgaat
//
LOCUS       DAZRUF010000218         6749 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZRUF010000218 DAZRUF010000000
VERSION     DAZRUF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.219"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(198..683)
                     /gene="sixA"
                     /locus_tag="ACHXOL_003517"
     CDS             complement(198..683)
                     /gene="sixA"
                     /locus_tag="ACHXOL_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR6810407.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(886..3030)
                     /gene="fadJ"
                     /locus_tag="ACHXOL_003518"
     CDS             complement(886..3030)
                     /gene="fadJ"
                     /locus_tag="ACHXOL_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR6810408.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3030..4340)
                     /gene="fadI"
                     /locus_tag="ACHXOL_003519"
     CDS             complement(3030..4340)
                     /gene="fadI"
                     /locus_tag="ACHXOL_003519"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR6810409.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4520..4804)
                     /locus_tag="ACHXOL_003520"
     CDS             complement(4520..4804)
                     /locus_tag="ACHXOL_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR6810410.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5176..6516
                     /gene="fadL"
                     /locus_tag="ACHXOL_003521"
     CDS             5176..6516
                     /gene="fadL"
                     /locus_tag="ACHXOL_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR6810411.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYISWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1674 a         1813 c         1616 g         1646 t
ORIGIN      
        1 cagacattat tcatttcaac atgtaacttt gggcaaaatc aagatacgcg cagaagaata
       61 atttccgaat gaaagtagaa agtgttttat ttatagatgg ggagagtctt cgatagtagt
      121 gagattgaca gatatccgct gcctgatgag caattatgcc caatgaatct acctcattgg
      181 gcataatttg gaactcatca gatagctttt gccatcttca gattacacgg gctcatctgc
      241 cagttgaatg tgccgtttcc gctttcatca agcgttacgc tggcaatggc cgaggtggta
      301 aacataggcg gcgtttcgcc cggacataac tcggcaacca gatacccgac taacggtaag
      361 tgagagatca ccagtaccga agcaacacct tcattggtca acgcctgcaa ataggcactg
      421 accagaccaa catcgccgca gggcgttaac tccggcagaa cttctgcact ggaaggcagg
      481 ttcagacaat cccctacttc ttccagtgtt tgctcggctc gcaggaacgg gctcaccaga
      541 acgcgttcga tttccacttt ttgacctttc agccagttcg ccatcaggcg agattcgtca
      601 caaccattag tggtcagagg acgaaccgaa tcactggcgg catcaagggc tgcgtcgccg
      661 tgacgcatga taaaaacttg catattgcac cgcttttgtt aaccagattc gtccgttttc
      721 tttcatctga aaccagagaa agaaaacggt ggccggcatt gtgccttatc cattcaccga
      781 atgaaacgct gttttttacc tcaatggcgt aagtatagtc aatatgcgtt tacattttga
      841 ccaacactcc gccattcagc gcggattcat atagctttga ccttcttatt gaaggtcagt
      901 tgcagttgtt ttccaaaaac tttccccacg cgcgcccatc tcgaccaaac gctcgcaagg
      961 ggtaaaacgg gaaccatact gcgtggccag tcgttgcatt attgcaacca cttcgcccgc
     1021 gccgagagaa tcgatatagc ggaacggtcc accgagaaat ggcggaaaac caatgccaaa
     1081 taccgcgcca atatccccgt cacgcacgct acggataacc tgctcatcaa cacaacgtac
     1141 tgcttcattc agcatcaaca tcacacaccg ttcagcaacc tgcggtgcgg agagtcgccc
     1201 ctgcccttgt gcgccaatga gcgggtaaat ggcgggatcg acctgttttt tgcttttacg
     1261 ccctttctga ccataaagat agaaaccccg accatttttt ctgcctttgc gatcgtcgtt
     1321 caaaattgaa gaaacaacat ttgcaggcgc gctaaaacgt tctccataag cggcttccag
     1381 tacaggaata attttagtcc cggtgtcgat tcctacctca tccaaaagtt ggattgggcc
     1441 taccggaaaa ccaaatttca ctagcgcggc atcaatgtgc tctacccgtt caccttcggt
     1501 cagcatgcgg atagcttcat taatgtaagg cgctaagatg cgattgacgt aaaacccggc
     1561 tttgtcacgc acgacaatgg gcgttttgcc ctgttttttc gccagtttta ccgtagtggc
     1621 gatggtttgc gccgatgtac ccgcatgagg aataatctct accagcggca ttttttccac
     1681 aggactgaag aaatgcaggc caataacttg ctcaggtcgc gcggcgtgag cggcgatatc
     1741 accaatcggt aaagatgacg tgttcgaagc aaaaatggta tgagcggcgc aattttgctc
     1801 cacttccgcc accatctgtt gttttaattc gagattttca aacaccgctt caataatcag
     1861 atcgcgatgg gcaaagccgc gatagtccgt cgttccggag attaatgcca gctgtttgtc
     1921 acgttcgctg gctttgagat gacgacggcg aactttgccc tccagctgat cccaactgta
     1981 cttcagcgca tgatttatgc cctgcgggtt gatatcttta attctgaccg gaagccccgc
     2041 tttacaagca gtgacataag caataccgcc gcccatcaag ccaccaccta aaattcccac
     2101 gctgtttaat ggcgcaggcg gtgcatcact accgggatct ttcttcacgt ccgtactggc
     2161 aaaaaagata ctacgcaaag cttgcgattg cggcgtcatc gccagttcgc caaaggcgcg
     2221 agcttcagcg tcatagccgc tgctggtgcc ctgcgctaat cccgtttcaa caacctccag
     2281 gatgcgttct gtcgccggat aattgccttg agttttgtgt tctgttttct tgccgaccat
     2341 tttgaacagc agcgcacgac ctaacggccc cgccagaata cgctcgcgta caggtagagg
     2401 gcgggaagat gggcgatcct tcttcgccag ctcaacagcg gcttccagca aaatggagtg
     2461 cggaacaacg tcatccacca gccccagctt taatgcctgt ttcgcccgaa gttgttttcc
     2521 ggtgagaatc atctctaatg ctgtactgac gcctatcaga cgcggtaaac gctgggtgcc
     2581 gcctgaaccg ggtaacaatc caagttgtac ttcaggcaaa ccgagcaccg ttttaggatc
     2641 gtcagtacaa acgcgaccgt ggcacgccag cgccagctcc agcccaccgc ccaggcaagc
     2701 gccatgaata gccgcgataa ccggaatggg caaagcatga atctccgcca tcaactgttg
     2761 gccctgccgc gccagtgctt ccgcttcttg cgccgttttg cagttgccaa tcatgttgat
     2821 gtctgctccg gcgataaaat tgtccggttt cgcggagata aacaccacgc cgcgcaactc
     2881 tttgttttca cggagttgct taataatggc gcgcacctgc gaggcaaatt ccgccttcag
     2941 ggtattcatt ttctcatccg gtacatcgat ggtgatgata gcaatgttgt ccagacgaac
     3001 attaagagta aacgctgatg ccatttccat tattccgcct ccagaaccat tgccgcgcca
     3061 agcccaccgg cagcacaggc ggtaactaaa ccaaatccac cgccgcgacg gcgaagttca
     3121 tgcaatgtct gggtaatcat ccgcgcgccg gtcgccgcga agggatgccc gtaagcaatc
     3181 gaaccgccaa gcacgttaaa tttgctatcg tccacttcgc cagtggcatg tgcacgcccc
     3241 agtacttcac gagcaaaacg ttcactaccc agcaactgaa tattcgccag tgtctgagct
     3301 gcaaaggctt cgtgcatatc gatcaatgtc agttcgctca tcgtcaaacc ggcacgttcc
     3361 agcgccagcg gtgttgacca ggctggaccg agcaacatgt cctgccagac atcaatcgca
     3421 gtaaatgcgt agctgcgcag ataccccagc ggcaccagcc ctaattcttt cgcccgggat
     3481 tcagtcatca ggatcaccgc tgccgcgcca tcggtcagcg gcgtactgtt tgccgccgtt
     3541 accgttccgt gtttgcgatc aaacgccggg cgcagctttg cgtaatcggc aagcgaggaa
     3601 ttaccgcgaa tattgttgtc ttcgacaagc ggttgtttat aaggagggat aaaggcagtc
     3661 atcacctctt ctttgagttt tccgtctgac catgcctgag cggcacgctg atgcgaacgg
     3721 tgcgctaatg catcttgctg ttctcgggtg atgccgtagg ttttcgccat ttgttcggcg
     3781 gtgtcgccca tccgcaagcc agtagaatat tctgctaccg caggcggtac gggcattaag
     3841 tcgcgcaaac gcaggcgaga gaagagtttc aggcgctggc tcatggttcg agctttgttg
     3901 acatcaacca gcacgcgcgc cagtttttta ctgacgccaa ttggcaatac ggaagaggaa
     3961 tctgccccac cggcaatccc cgctcggata gttcccgcca tcaggctttc tgcgacgttt
     4021 gcaaccgcct ggaaactggt agcgcaagcg cggctgacgc tgtaagcatc ggtatgcaca
     4081 ttcattccgg taccgagaac aatttcacgc gcaatgttgg gggcttcagg catttgtacg
     4141 acctgaccaa agaccagctg ttcaatcact tcagcgggga tctcgctgcg tgccagcagt
     4201 tcgcctacca ccatcttccc taaatcaacc gcgggaatgc catgaaaagc cgtcgcctgg
     4261 cgggcaaaag gcgtacgtaa accgctaaca atggcgatac gatcgccctg gcgggtaacc
     4321 agcggtaaaa cctgacccat aacactcccc tgtaaaaaaa taaataaagt ggtctgacct
     4381 gatcatagtc ttaactattt ttttacattt agccaagcgg agaaaaagga aagtgggagc
     4441 tatgacacag tgaagacagg agaaagaaaa gaaaacgccc ctgccagcaa ctgacagggg
     4501 cgaacgtacc cgagaggaat taacgcagac ccagctggaa gatcagcatt tcagcttcgc
     4561 aggcaaaagt gaaatcgata tccaggcgca caccgtcaga ctcttcagtg aaagtcgggg
     4621 agattttgca cggttcggat tccacgctac gggctttttc agtcagcgcc gccagcgttt
     4681 gttctgcttc tgcgcggttt gcaaacacgc ggctgtaaga cgcggtgcag tcggagttgt
     4741 ccataatggt gccaacatcc atacagcagc aaaccggggt ttcatcagca ctacatttac
     4801 tcatcgttga tttcctctgt atgtgcaccc aaggtgccag ataaacgttg tggatatttt
     4861 acgcttccgg aaagtgctgc tccagttgtt aattctgcaa aatcggataa gtgaccgaaa
     4921 tcacacttaa aaatgatcta aaacaaaatt cacccgaatc cgcgagtgcg caacctccaa
     4981 attttgccag ctggatcgcg tttcttagat catatttgaa aaaagatgga aacatacttg
     5041 cagcattcca gctggtccga cctatactct cgccactggt ctgatttcta agatgtacct
     5101 cagaccctac acttcgcgct cctgttacag cacgtaacat agtttgtata aaaataaatc
     5161 attgaggtta tggtcatgag ccagaaaacc ctgtttacaa agtctgctct cgcagtcgca
     5221 gtggcactta tctccaccca ggcctggtcg gcaggctttc agttaaacga attttcttcc
     5281 tctggcctgg gccgggctta ttcaggggaa ggcgcaattg ccgatgatgc aggtaacgtt
     5341 agccgtaacc ccgcattgat taccatgttt gaccgcccga cattttctgc gggtgcggtt
     5401 tatattgacc cggatgtgaa tatcagcgga acgtctccgt ctggtcgtag cctgaaagcc
     5461 gataacatcg cgcctacggc atgggttccg aacatgcact ttgttgcacc gattaacgac
     5521 caatttggtt ggggcgcttc tattacctct aactatggtc tggcaacaga gtttaatgat
     5581 acttatgcag gcggctctgt cgggggtaca actgaccttg aaaccatgaa cctgaactta
     5641 agcggtgcgt atcgcttaaa taatgcatgg agctttggtc ttggtttcaa cgccgtctac
     5701 gctcgcgcga aaattgaacg tttcgcaggc gatctggggc agttggttgc tggtcagatt
     5761 atgcaatctc ctgccgggca aactcctcaa gggcaagcat tggcagctac cgccaacggt
     5821 atcgacagta ataccaaaat cgctcatctg aacggtaacc agtggggctt tggctggaac
     5881 gccggaatcc tgtatgaact ggataaaaat aaccgctatg cactgaccta ccgttctgaa
     5941 gtgaaaattg acttcaaagg taactacagc agcgatctta atcgtgcgtt taataactac
     6001 ggtttgccaa ttcctaccgc gacaggtggc gcaacgcaat cgggttatct gacgctgaac
     6061 ctgcctgaaa tgtgggaagt gtcaggttat aaccgtgttg atccacagtg ggcgattcac
     6121 tatagcctgg cttacatcag ctggagtcag ttccagcagc tgaaagcgac ctcaaccagt
     6181 ggcgacacgc tgttccagaa acatgaaggc tttaaagatg cttaccgcat cgcgttgggt
     6241 accacttatt actacgatga taactggacc ttccgtaccg gtatcgcctt tgatgacagc
     6301 ccagttccgg ctcagaatcg ttctatctcc attccggacc aggaccgttt ctggctgagt
     6361 gcaggtacga cttacgcatt taataaagat gcttcagtcg acgttggtgt ttcttatatg
     6421 cacggtcaga gcgtgaaaat taacgaaggc ccataccagt tcgagtctga aggtaaagcc
     6481 tggctgttcg gtactaactt taactacgcg ttctgataac gcgttcgcct ggataaagtc
     6541 acctgcatag caggtgactt taactccccc acttcaccga aagtagtgtc ctcattgctt
     6601 acctcctgag ttttgcaaac agcctgttgg cagcttgccc tttcaaatca ataagcggta
     6661 tccacagaca aacctgaagg gaaaggcata ttttcaggcg ttctgctcgt ccttctcaaa
     6721 gagtttactt ttctgcattt ccaggatac
//
LOCUS       DAZRUF010000219         6594 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZRUF010000219 DAZRUF010000000
VERSION     DAZRUF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.220"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..967)
                     /gene="rfaC"
                     /locus_tag="ACHXOL_003522"
     CDS             complement(<1..967)
                     /gene="rfaC"
                     /locus_tag="ACHXOL_003522"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR6810412.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAM"
     gene            complement(971..2017)
                     /gene="rfaF"
                     /locus_tag="ACHXOL_003523"
     CDS             complement(971..2017)
                     /gene="rfaF"
                     /locus_tag="ACHXOL_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR6810413.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(2027..2959)
                     /gene="rfaD"
                     /locus_tag="ACHXOL_003524"
     CDS             complement(2027..2959)
                     /gene="rfaD"
                     /locus_tag="ACHXOL_003524"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR6810414.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            3173..4369
                     /gene="kbl"
                     /locus_tag="ACHXOL_003525"
     CDS             3173..4369
                     /gene="kbl"
                     /locus_tag="ACHXOL_003525"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR6810415.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            4379..5404
                     /gene="tdh"
                     /locus_tag="ACHXOL_003526"
     CDS             4379..5404
                     /gene="tdh"
                     /locus_tag="ACHXOL_003526"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR6810416.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            5643..6545
                     /locus_tag="ACHXOL_003527"
     CDS             5643..6545
                     /locus_tag="ACHXOL_003527"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR6810417.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMW"
BASE COUNT         1567 a         1645 c         1706 g         1676 t
ORIGIN      
        1 tcatagcagc gttttcttca atgaatcgct taacagcatt tgctgtcaat tgagacaact
       61 cattccccgg agccctacaa accatctgat tcttcccata tccaccaatt aatcccggat
      121 cggttggtcc ataaaccgtg atattgggtc tatccagtgc cgccgttaaa tggcttaacc
      181 ccgtatccac cgacactaca aatttagccc cggccagcac gcgggcaacg ccttccagac
      241 tcatcttcgg caatacttca acataagcaa atccttccgc cagtcgtttc gcccgctctt
      301 cctcatgcgg cgcgccccac ggaagtttaa tccgtattcc tgaatcagcc agtaaaccaa
      361 tcaattctcg ccagtgttct tccggccagt gtttatcatc acgggtcgtc gcatgaagaa
      421 atacggcata ttcgccagca tctgtaggca ggttcgtcag gaaatgctgt gcaatagcat
      481 aatcgccctg ggtttgcggt ttgctatagc ccagactttt ggcaaacagt tcgcgggtgc
      541 gttctacggc gtgctgctgt tttgcaatat gatgcttacg attgtaaaac agactggcta
      601 aaggttcgcg agcggtttgc cagtccatgc catgctttac gccatgcgcc agacgcgtca
      661 ccagcgccgc gctttttacc agcccctgag cgtcgataac tgcgtcatag ttctctgctt
      721 gtagcgcttc acgaaacgct ttgcgttccg cttttatggg ggccgagaac caggctttac
      781 gccagcgacg tattgccaca ggaataactc gctcaacggc ggcgtgccag gaaggaatct
      841 gtgcgaaccc ttcttccacc acccagtcaa acttaatccc tgggattgcc tgctgggcat
      901 cagtgagtgc gggcaacgta tggagaacat cgcccatcga cgatgtttta acgatcaaaa
      961 cccgcatccg tcaggcttcc tcttgtaaca atagcgcgtt gagttcttcc agtacgcgct
     1021 ggggagtaat gtcgattaag ctctggtgat aaccctccgc agcgtcacct ttacgcactt
     1081 tgtgatagcc ggtaatcagg cggatcacgc gcgctttatg ggatagcggc ggtgtgaagt
     1141 ccgggctact cggaccatac agggcaacca gcggacgatt gagcgccgcc gcaacgtgca
     1201 tcaggccaga atcgttagtg acaatggctt tacaggctgc aatcaggata accgcttgat
     1261 caagctgtgt ttcccccgcc aggttccgac accatgcctg ctgctcggta ttcaaagcgg
     1321 caagaatctc attgcccgct tcatgatctt tcgccgagcc aaacagaacc acctgataac
     1381 cttcatcaat cagctgcttt gccagttccg catagtggta gtgtggccag cgttttgccg
     1441 gaccaaactc cgcacccggg caaaaaccaa tcatcggacg ttctgatgaa agcgaaaatt
     1501 gattacaggt atatgatttt tcaccttcgc tcacctgcaa ttgcggccat aacaacggtt
     1561 ttggtagatc ttgtgcggta cgcatcacgc ctttgtcata ggccagcgcg acatagcgtt
     1621 ccaccattag cggccaggct tctttatcga gcacgcgtaa atcgttgagt aaaccgtagc
     1681 gcatctcgcc gcgccagccg gtgcgatgag gaatacccgc gaagaaaggc actaatgcag
     1741 atttgaagga gttgggtaag acgtaggcgc ggtcgtagcg cttttcacgc aggctatgac
     1801 ccagtttgcg gcgttcgccg atttccagcg ctccgtgacc gagaggcata ggaatagctt
     1861 cgttaacttc cggcatccgc gataataatg gacggcacca tgccggtgcc atcacatcga
     1921 ttatcgcctg gggatagcgc gcctggagcg tgcgatagag actttgcgac atcatcatgt
     1981 cgccaaccca agacgggccg atcaccagta ttttcatgca gagctcttat gcgtcgcgat
     2041 tcagccaggc catgtattcc gttacacctt cagcaacggt tttgaacggt ttgtcgtaac
     2101 ccgccgcgcg cagatttgtc agatctgcct gagtgaacgc ctggtagcgg cctttcagct
     2161 tatccgggaa cggaatgtat tcgatctggc ctttcttgtg ataagccagc gtagcatcag
     2221 ctacagcctg gaaggattcc gcacgaccag taccgaggtt gaagatgccg gaaacgccat
     2281 tttccaggaa ccacagattc acatcagcca cgtcgcccac atagacgaag tcgcgtttga
     2341 agttctcgct accttcaaac agcttcggtg attcaccgtt gttaagctgg gtgttgagat
     2401 ggaaagcgac gctcgccatg ctgcctttat ggccttcacg cggtccataa acgttgaaat
     2461 agcggaagcc aacaatctgc gagttagctt caggcaggat ttggcgaaca tattcatcaa
     2521 acaggaattt tgagtaaccg tagacgttca acggtttttc gtactcgcgg gattcaataa
     2581 agtcggaggt gcgtccgccg taggtggctg cggaagaggc atacaggaac gggatttcgc
     2641 gctccagaca gtagtgcagc agctctttgg agtattgata gttgttatcc atcatatact
     2701 tgccatccca ctcggtggtg gaagagcacg cgccttcgtg gaaaatcgct tcgacatcgc
     2761 cgaactcttc gccagccata atctggatca ggaagtcttc cttatccata tagtccgcga
     2821 tatccagatc caccaggttc acaaacttgg tgccgtcttt caggttgtcc accaccagaa
     2881 tatcggtgat gcctttatca ttcagggcct taacgatgtt gctgccgata aagcccgcgc
     2941 cgccggtaac gatgatcata actgtaacct tcgaattatg gagtcagaga caatctcaga
     3001 cacgaatact tctatcatat cactacacgg cgttggcttc agccattcgc caaaagtgag
     3061 attgatggac gttatttatc ctgaattttg cagaagtgtt aacgcgtcat ctcgtcgcga
     3121 cctataagtt tgggtaatat gtgctggaat ttgccctgtc tggagaatcg caatgcgtgg
     3181 agaattttat cagcagttaa ccaacgatct ggaaaccgca cgggcggaag ggttgtttaa
     3241 agaagagcgc attattacgt ctgcgcagca agcagatatc actgtggctg atggaagcca
     3301 cgtcattaac ttttgtgcca ataactatct cgggctggcg aattatccgg atctgattgc
     3361 ggcggcaaag gcgggaatgg attctcacgg tttcggcatg gcttcggtgc gttttatttg
     3421 cggcactcag gacagccata aagagcttga acaaaaactg gcggcattcc tggggatgga
     3481 agatgcgatt ctctactctt cctgctttga tgctaacggt ggcctgtttg aaacgctgct
     3541 gggcgcggaa gacgccatta tctccgacgc actgaaccac gcgtctatta ttgatggtgt
     3601 gcgtctgtgt aaagctaaac gctatcgcta tgccaacaac gatatgcagg agctggaagc
     3661 acgtctgaaa gaagcgcgtg aagccggtgc gcgtcatgtg ctgattgcca ccgatggtgt
     3721 gttctcgatg gacggcgtga ttgccaacct gaagggcgtt tgcgatctcg cagataaata
     3781 tgatgccctg gtgatggtag acgactccca cgcggtcggt tttgtcggtg aaaatggtcg
     3841 tggttcccat gaatactgcg atgtgatggg ccgggtcgat attatcaccg gcacgctcgg
     3901 taaagcgctg ggcggggctt ctggtggtta taccgcggcg cgcaaagaag tggttgagtg
     3961 gctgcgccag cgttctcgtc cgtacctgtt ttccaactcg ctggcaccgg ccattgttgc
     4021 cgcatccatc aaagtactgg agatggtcga agcgggtagc gagctgcgtg accgtctgtg
     4081 ggcgaacgcg cgtcagttcc gtgagcaaat gtcggcggcg ggctttaccc tggcgggagc
     4141 cgatcacgcc attattccgg tcatgcttgg tgatgcggta gtggcgcaga aatttgcccg
     4201 tgagctgcaa aaagagggca tttacgtcac cggtttcttc tatccggtcg ttccgaaagg
     4261 tcaggcgcgt attcgtaccc agatgtctgc ggcgcatacc cctgagcaaa ttacgcgtgc
     4321 agtagaagca ttcacgcgta ttggtaaaca actgggcgtt attgcctgag gatgtgagat
     4381 gaaagcgtta tccaaactga aagcggaaga gggcatctgg atgaccgacg ttcctgtacc
     4441 ggaactcggg cataacgatc tgctgattaa aatccgtaaa acagccatct gcgggactga
     4501 cgttcacatc tataactggg atgagtggtc gcaaaaaacc atcccggtgc cgatggtcgt
     4561 gggccatgaa tatgtcggtg aagtggtagg tattggtcag gaagtgaagg gcttcaagat
     4621 tggcgatcgc gtttctggcg aaggccatat cacctgcggt cactgtcgca actgtcgtgg
     4681 cggtcgtacc catctgtgcc gcaacacgat cggcgtcggc gttaaccgcc cgggctgctt
     4741 cgccgaatat ctggtgattc cggcgttcaa cgccttcaaa attcccgaca atatctccga
     4801 cgacctggct tccatttttg atcccttcgg taacgccgtg cataccgcgc tgtcgttcga
     4861 tctggttggc gaggatgtgc tggtctctgg tgcaggcccg attggtatta tggcagcggc
     4921 ggtggcgaaa cacgttggtg cacgcaatgt ggtgatcact gatgttaacg aatatcgtct
     4981 cgaactggcg cgcaaaatgg gtatcacccg tgcggtcaac gtcgctaaag aaaaccttaa
     5041 tgatgtgatg gcagaactgg gcatgaccga aggctttgat gtcggtctgg aaatgtccgg
     5101 tgcgccgcca gcgtttcgta ccatgctcga caccatgaac cacggcggtc gcattgcgat
     5161 gctgggtatt ccaccgtccg atatgtctat cgactggacc aaagtaatct ttaaaggctt
     5221 gttcattaaa ggtatttacg gtcgtgagat gtttgaaacc tggtacaaga tggcggcgct
     5281 gattcagtca ggtctggatc tctcgccgat cattacccat cgtttctcta tcgatgattt
     5341 ccagaagggc tttgacgcta tgcgttcggg ccagtccggg aaagtaattc tgagctggga
     5401 ttaacacgaa caagggctgg tattccagcc cttttgcctg aggataatcc gttaaatatg
     5461 taaaatcctg tcagtgtaat aaagatatcg taattgcgct gatctcttat atagctgctc
     5521 tcattatctc tctaccctga agtgactctc tcacctgtaa aaataatatc tcacaggctt
     5581 aatagtttct taatacaaag cctgtaaaac gtcaggataa cttcagaggt cgtcggtaat
     5641 ttatgatgaa cagcaccaat aaacttagtg ttattattcc gttatataat gcgggcgatg
     5701 atttccgcac ttgtatggaa tctttaatta cgcaaacctg gactgctctg gaaatcatta
     5761 ttattaacga tggttcaacg gataattctg ttgaaatagc aaagcattac gcagaaaact
     5821 atccgcacgt tcgtttgttg catcaggcga atgctggcgc atcggtggcg cgtaatcgtg
     5881 ggattgaagt ggcaacgggc aaatatgtcg cttttgtcga tgctgacgat gaagtctatc
     5941 ccaccatgta cgaaacgctg atgaccatgg cgttagagga cgacctcgac gtggcgcagt
     6001 gcaacgctga ctggtgtttt cgtgaaacgg gagaaacctg gaaatccatc cccagcgatc
     6061 gccttcgctc aaccggcgta ttaaccggcc cggactggct gcggatgggg ctttcttcgc
     6121 gccgttggac tcacgttgtc tggatggggg tttatcgccg cgatgttatt gttaaaaata
     6181 acattaaatt tattgccgga ttacatcatc aggatattgt ctggacaaca gaatttatgt
     6241 ttaatgcgct gcgtgcgcga tataccgagc aatcgttata taaatattat ctgcataata
     6301 cgtcagtgag tcggttgcat agacaaggga acaaaaacct taattatcaa cgtcactata
     6361 ttaagattac tcgtctgcta gagaaattaa atcgaaatta tgccgacaaa attacgattt
     6421 atccggaatt tcatcagcaa ataacttacg aagcattgcg tgtttgccat gcggtgcgca
     6481 aagagccgga tattattacc cgccaacgga tgattgccga gatatttact tccggtatgt
     6541 ggtaatgact ccaacttatt gatagtgttt tatgttcaga taatgcccga tgac
//
LOCUS       DAZRUF010000220         6557 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZRUF010000220 DAZRUF010000000
VERSION     DAZRUF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.221"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            145..1320
                     /gene="mdtL"
                     /locus_tag="ACHXOL_003528"
     CDS             145..1320
                     /gene="mdtL"
                     /locus_tag="ACHXOL_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR6810418.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1295..2254
                     /gene="yidZ"
                     /locus_tag="ACHXOL_003529"
     CDS             1295..2254
                     /gene="yidZ"
                     /locus_tag="ACHXOL_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR6810419.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2411..3160
                     /locus_tag="ACHXOL_003530"
     CDS             2411..3160
                     /locus_tag="ACHXOL_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR6810420.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3182..3748
                     /gene="yieF"
                     /locus_tag="ACHXOL_003531"
                     /gene_synonym="chrR"
     CDS             3182..3748
                     /gene="yieF"
                     /locus_tag="ACHXOL_003531"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR6810421.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3802..5139)
                     /gene="adeP"
                     /locus_tag="ACHXOL_003532"
     CDS             complement(3802..5139)
                     /gene="adeP"
                     /locus_tag="ACHXOL_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR6810422.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5305..5970
                     /gene="yieH"
                     /locus_tag="ACHXOL_003533"
     CDS             5305..5970
                     /gene="yieH"
                     /locus_tag="ACHXOL_003533"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR6810423.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6037..6510
                     /locus_tag="ACHXOL_003534"
     CDS             6037..6510
                     /locus_tag="ACHXOL_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR6810424.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1611 a         1656 c         1658 g         1632 t
ORIGIN      
        1 taaatttggc taaatccttc aagaagccag ccattcgctg gcttcttgcc tctcaggaaa
       61 tcacttatgt ccaaatggca actcgcctga tcctccttca ccacgtatgc tttgcgtcac
      121 cttactatca ggacgcttta gcccatgtcc cgctttttga tttgtagttt tgccctggtt
      181 ttactttatc ccgccgggat tgatatgtac ctcgttggtt taccgcgcat cgccgccgat
      241 ctcaatgcca gcgaagcgca gttgcatatt gcgttctccg tatatctggc ggggatggca
      301 gctgcgatgt tatttgccgg taaagtggct gatcgttcag ggagaaagcc ggtcgccata
      361 cccggcgcgg cgctatttat tattgcctcg gtgttctgtt cactggctga aaccagcacg
      421 ttatttcttg caggccgatt tctacagggg ttgggcgcag gctgttgtta cgtagtggcg
      481 ttcgccattt tgcgcgacac gctggatgat cgacgtcggg ctaaagtgct gtcattactc
      541 aacggtatta cctgcatcat tccggtgtta gcgccagtgc tcggacatct gattatgctt
      601 aaattcccgt ggcagagtct gttctgggcg atggcaatga tgggcatcgc ggtactgatg
      661 ttgtctttgt ttattttaaa agaaacgcgc ccagcgtccc ccgctgcttc ggacaaacca
      721 cgagaaaata gcgagtcgct gcttaatcgg tttttcctca gccgtgttgt tatcaccacc
      781 ctcagcgttt cggtgatcct cactttcgtc aatacatcgc cggtattgct gatggaaatc
      841 atgggttttg agcgcggaga atacgccacc attatggcgt tgaccgctgg cgtcagcatg
      901 accgtttcat tctccacgcc atttgcgctg ggaattttta agccacgtac gttgatgatc
      961 acctcgcagg tgttattcct tgcggcgggg atcacccttg ccgtttcacc ttcccatgcg
     1021 gtttctctgt ttggtatcac gctgatttgc gccggtttct cggtaggttt tggcgtagcg
     1081 atgagtcagg cgttaggacc attttcatta cgcgcgggcg tagccagctc gaccttaggt
     1141 attgcgcagg tttgcggttc gtcactgtgg atttggctgg cagcggtggt tggtattggc
     1201 gcatggaata tgctgatcgg gattctgatt gcctgtagta tagtgagcct gttgctgatt
     1261 atgttcgtcg cgcctggacg ccccgttgcc gctcatgaag aaatccatca ccacgcttga
     1321 tctcaatctg ctgctctgtc tgcaactgct gatgcaggag cgcagcgtga ccaaagcggc
     1381 gaagcggatg aacgtgaccc cttcagcggt gagtaagtcg ctggcaaagt taagagcgtg
     1441 gtttgacgac ccgctctttg tgaactcacc gctgggcctg tcgcccacac cgctaatggt
     1501 cagcatggag caaaatctgg cggaatggat gcaaatgagc aatctgctgc tggataaacc
     1561 gcaccaccag acaccgcgcg gcctgaagtt tgagttggcg gcggaatcgc cgctgatgat
     1621 gatcatgctt aatgcgctgt cgaaacggat ctaccaacgt tacccgcagg cgaccatcaa
     1681 actacgtaac tgggattacg attccttaga tgccattact cgtggtgaag tggatattgg
     1741 tttttccggt cgcgaaagcc atcctcgctc gcgggagctg ttaagctcgc taccgttagc
     1801 cattgattat gaagtgctgt ttagtgatgt accctgcgtc tggttacgca aagatcatcc
     1861 ggcactgcat gaaacgtgga atctggacac ctttttacgt tatccgcata tcagcatttg
     1921 ctgggaacag agcgacacct gggcgctgga caatgtgtta caggagctgg ggcgcgaacg
     1981 cacgattgct atgagtctgc cggaattcga gcagtcactg tttatggcag cgcaacccga
     2041 caatctgcta ctggcgaccg cgccgcgcta ctgtcagtac tacaatcaac tccatcaact
     2101 gccgttggtt gctcttcctc tcccgtttga cgaaagccag caaaaaaagc tggaagttcc
     2161 ttttaccctg ctgtggcata aacggaacag ccataatccg aagatcgtct ggttacggga
     2221 aaccattaaa aacctttacg cgtcgatggc ataaccgaat cgtatgaaac gcgacccaat
     2281 ttcacaataa aatgtaaaaa agttgtaaca agcttgtctg aatcgaactt ttagccgctt
     2341 tagtctgtcc atcattccag taaatgatta ctcttgtatt cataatggac cattaagcat
     2401 ggagcgaaaa atggcgactc actttgcccg agggatttta acggaaggac atctgatttc
     2461 tgttcgtctc ccctcccagt gtcatcaaga agcccgaaac attccccctc atcgtcaaag
     2521 ccgttttctg gcgtccagag gtttactcgc agaactgatg ttcatgctgt atggcattgg
     2581 cgaattgccg gaaatcgtca ccctgccgaa aggtaaaccg gttttcagtg ataaaaattt
     2641 gccttcgttt tctatttcct atgccgggaa tatggttggc gtggcgttaa caaccgaagg
     2701 tgaatgtggc ctcgatatgg aactacagcg tgcgacgcgc gggtttcata gcccacacgc
     2761 gcccgataac cacacctttt ccagcaatga atcgctatgg atcagtaaac aaaacgatcc
     2821 taacgaagcg cgggcgcagc tcatcacgct gcgccgaagc gtgctaaaac taaccggtga
     2881 tgttttgaat gacgatccgc gcgatctgca gctgctgccc attgccggac gcctgaaatg
     2941 tgctcatgta aatcatgtag aagcgttatg cgacgcggag gacgtgctgg tgtggtccgt
     3001 ggcggtcacg cccgcgattg aaaagctcag tgtctgggag ttagatggca aacacagctg
     3061 gaaaagcctg ccggatattc acagccgcgc caacaatcct accagccgga tgatgcgttt
     3121 tgcccagctc tctaccgtga agtctttttc gccaaattga tagacaacca caggagtcat
     3181 tatgtctgaa aaattgcagg tggttacgtt actggggagc ctgcgcaaag gctcatttaa
     3241 tggcatggtt gcacgtaccc tgccgaaaat tgctccggcg agcatggaag tcaatgcgtt
     3301 accttccatt gccgacattc ccctgtatga cgctgacgta cagcaggaag aaggttttcc
     3361 agcaacggtt gaagctctgg cggaacagat ccgtcaggct gacggtgtgg tgatcgtcac
     3421 gccggaatat aactactcgg taccgggtgg gctgaaaaat gccatcgact ggctttcccg
     3481 cctgccggat caaccgctgg ccggtaaacc ggtgttgatt cagaccagct caatgggcgt
     3541 gattggcggc gcgcgctgtc agtatcacct gcgccagatt ctggttttcc tcgatgcaat
     3601 ggtgatgaac aagccggaat ttatgggcgg cgtgattcag aacaaagttg atcggcaaac
     3661 cggagaagtg attgatcagg gtacgctgga ccacctgacc gggcaattga ccgcatttgg
     3721 tgagtttatt cagcgagtta agatctaaat aaaaaacccg ccagtaatca tgcatggcgg
     3781 gtttttaatg cgctatcgat tttaatgagc gtcgataaat acaatcttca ggataaacag
     3841 cagcgcaacg atgattacgc acgggctaag gtcacgcaga cgcccggtac caatcttcat
     3901 cacgcagtag gagataaagc ccagcgcaat accttcggta atcgaaaagc tgaacggcat
     3961 catcacggcg gtaataaacg ccggaacaga ttcagtaaga tcctgccagt tcacgcgagc
     4021 aagacttgag gtcatcaaca cgccaacgta gatcaacgcg ccagctgcag cgtagcctgg
     4081 caccattccc gccagcggcg acagaaagat aaccagcagg aacagcagac caacaaccac
     4141 tgccgtcaga ccggtacgac cgccaaccga tacgccggaa gaggactcaa tataagccgt
     4201 aacggaagaa gtaccgataa acgaaccggt cacggaagag atactgtcga catacagcgc
     4261 ctgcttcatg cgcgggaatt tccccttctc atccgccaga cctgctttat cggtcacgcc
     4321 aatcagcgta ccggaggagt caaacaagtt gaccaacatg aaagagaaaa tcacccctgc
     4381 cagcccgagg ttaaacgacc cggctaaatc tacatgaccc acaactgtca ttacgctcgg
     4441 cggcgcagaa acgatgccat tgtagtgcac atcacccagc atccagccca gcagcgtcgt
     4501 caccacgata gaaaccagca ccgctgcgtg aatgttgcgc gaggccagaa tggcgatgat
     4561 gaagaaaccg aggatcccca gcagcacgct gtgagaagtc agattaccga tgctcaccag
     4621 cgtttccggg ttagcgacaa tcacacctgc gtttttcagc cccatcatgc caatgaacag
     4681 accgataccg ctggtaatac ccacacgcag acttaccgga atgttagcaa tcatccagta
     4741 acgaacgcgg aaaatggtca aaagaagcag tccgacagcg ccccagaaga ttgcgcccat
     4801 accgatctgc cacggcagcc ccattgcctg gacaaccaca aaagcgaaaa aggcgttaag
     4861 ccccatcgca ggagccagcg ccaccggaag gtttgcaaac aatcccatca taatgctacc
     4921 gaaagcagca atcagacagg ttgttacgaa gacggcactg gtgtccatgc cagcaacgcc
     4981 cagaatttgt gggttaacaa atacgatgta gaccatcgtc aggaaggtgg tgaacccggc
     5041 aatcacttca gttcgtgccg tagtaccatg ttcgcgcagt ttaaaaactc gttccagcat
     5101 cccctgacca gaagtctggg tggtatgttg atgactcatt atctatttcc gaacaaggag
     5161 ggaaaatccg tcgctatcgt ataccaaaat gcgacaatag gcgcgtttgt gagagacttt
     5221 ttttattgga tttacttata cggcaacgat tgcgttgcgc aaatcggctc tacgaaaacg
     5281 ttaaactgat taaaaaggaa aggcatgtcc cggatagaag cggtattttt cgactgcgac
     5341 ggtacgctgg tcgacagtga agtcatttgc tcccgcgcat atgtaacgat gtttcaggaa
     5401 tttggtatta cgctcgatcc tgaagaggta ttcaaacgtt tcaaaggtgt aaaactgtac
     5461 gaaattatcg atattgtttc ccttgaacat ggtgttacgt tagcgaaaac agaatctgaa
     5521 cacgtttacc gtgcagaagt cgctcggctg ttcgattcag aactggaagc catcgaaggg
     5581 gctgaagcgc tcctgtcagc gatcactgcg ccaatgtgtg tggtatctaa cggcccaaat
     5641 aacaaaatgc agcattctat gggcaagctg aatatgttgc actacttccc ggataaactg
     5701 ttcagcggct acgatattca gcgctggaag ccagacccgg cgttaatgtt ccatgcggca
     5761 aaagcgatga atgtaaatgt agaaaactgc attctggttg atgactcagt tgccggtgcg
     5821 caatctggta tcgacgcagg tatggaagtg ttctacttct gcgccgaccc gcacaataag
     5881 ccgatcgttc acccgaaagt caccaccttt acccatcttt cgcagttacc ggaactgtgg
     5941 aaagcgcgtg gttgggatat tacggcatag ttcttcacac tcccttcact taccccgctt
     6001 aaattggcgc tcaaaggtaa gtaaagggag tttgatatgt ctgtttcacg tcgggtaata
     6061 catcacggac tttattttgc ggttttagga ccgttaattg gtgttctgtt tcttgtcctc
     6121 tacatattct tcgcaaaaga accgctggtt cttttggtaa taatacaggt acttccattg
     6181 tttctcttat tgtcgattac tacgggagct attcctgcat tgttaaccgg cgtgatggtc
     6241 gcctgtctgc cggaaaagat cgggtcacag aaaaattatc gttgtctggc tggtggcata
     6301 ggtggcgtcg ttatcaccga gatctattgt gcagttattg tacatattaa gggcatggct
     6361 tcctcggagt tgtttgaaaa cattctttct ggtgacagtc tcgttgtccg catcattcct
     6421 gcattgctgg caggtgtggt gatgagcaga atcattaccc gtctgcccgg attggatatt
     6481 tcatgtcctg aaacagactc tttaagttaa gcgggatact ttatctttgg gctactcaaa
     6541 agcagacagg atgttac
//
LOCUS       DAZRUF010000221         6500 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZRUF010000221 DAZRUF010000000
VERSION     DAZRUF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.222"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="ACHXOL_003535"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="ACHXOL_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="ACHXOL_003536"
     CDS             complement(1751..2509)
                     /locus_tag="ACHXOL_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR6810425.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHXOL_003537"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHXOL_003537"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR6810426.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHXOL_003538"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHXOL_003538"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR6810427.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="ACHXOL_003539"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="ACHXOL_003539"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR6810428.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="ACHXOL_003540"
     CDS             complement(5474..6079)
                     /locus_tag="ACHXOL_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810429.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..>6500)
                     /locus_tag="ACHXOL_003541"
     CDS             complement(6082..>6500)
                     /locus_tag="ACHXOL_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810430.1"
                     /translation="QRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINSV
                     PGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIENP
                     LKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
BASE COUNT         1639 a         1694 c         1663 g         1504 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgccagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattt ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta
//
LOCUS       DAZRUF010000222         6468 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZRUF010000222 DAZRUF010000000
VERSION     DAZRUF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.223"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHXOL_003542"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHXOL_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR6810431.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHXOL_003543"
     tRNA            1230..1305
                     /locus_tag="ACHXOL_003543"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHXOL_003544"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHXOL_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR6810432.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHXOL_003545"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHXOL_003545"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR6810433.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHXOL_003546"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHXOL_003546"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR6810434.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHXOL_003547"
     CDS             complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHXOL_003547"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR6810435.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4868..5305)
                     /locus_tag="ACHXOL_003548"
     CDS             complement(4868..5305)
                     /locus_tag="ACHXOL_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR6810436.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5523..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5787..6463)
                     /locus_tag="ACHXOL_003549"
                     /pseudo
     CDS             complement(5787..6463)
                     /locus_tag="ACHXOL_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
BASE COUNT         1726 a         1751 c         1419 g         1572 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     4321 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     4381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     4441 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     4501 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     4561 cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     4621 atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc caataagcgc
     4681 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     4741 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     4801 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     4861 caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt gttaacgtcc
     4921 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
     4981 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
     5041 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
     5101 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
     5161 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
     5221 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
     5281 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
     5341 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
     5401 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
     5461 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
     5521 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
     5581 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
     5641 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
     5701 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
     5761 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
     5821 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
     5881 ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac cgagatttgt
     5941 tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca atcaacttca
     6001 acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg cggaattcat
     6061 taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg accaaatcga
     6121 catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa tgtccggcga
     6181 ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata caaggctgat
     6241 cggatttaga ggcttccagg cccccacaac gatcgctcat gcgacatcga gtgcttagct
     6301 gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg gaaatttcat
     6361 cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa tcctgcgcga
     6421 ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgataa
//
LOCUS       DAZRUF010000223         6462 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZRUF010000223 DAZRUF010000000
VERSION     DAZRUF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.224"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..699
                     /locus_tag="ACHXOL_003550"
     CDS             <1..699
                     /locus_tag="ACHXOL_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR6810437.1"
                     /translation="TPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARL
                     QRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVS
                     NMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYT
                     SQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQSGR
                     PLKQEPTEMIQATA"
     gene            complement(739..1410)
                     /locus_tag="ACHXOL_003551"
     CDS             complement(739..1410)
                     /locus_tag="ACHXOL_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR6810438.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1517..1750)
                     /gene="yedF"
                     /locus_tag="ACHXOL_003552"
     CDS             complement(1517..1750)
                     /gene="yedF"
                     /locus_tag="ACHXOL_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR6810439.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1747..2952)
                     /gene="yedE"
                     /locus_tag="ACHXOL_003553"
     CDS             complement(1747..2952)
                     /gene="yedE"
                     /locus_tag="ACHXOL_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR6810440.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            3139..3552
                     /gene="yedD"
                     /locus_tag="ACHXOL_003554"
     CDS             3139..3552
                     /gene="yedD"
                     /locus_tag="ACHXOL_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR6810441.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3586..5073)
                     /gene="amyA"
                     /locus_tag="ACHXOL_003555"
     CDS             complement(3586..5073)
                     /gene="amyA"
                     /locus_tag="ACHXOL_003555"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR6810442.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(5151..5516)
                     /gene="fliT"
                     /locus_tag="ACHXOL_003556"
     CDS             complement(5151..5516)
                     /gene="fliT"
                     /locus_tag="ACHXOL_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR6810443.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5516..5926)
                     /gene="fliS"
                     /locus_tag="ACHXOL_003557"
     CDS             complement(5516..5926)
                     /gene="fliS"
                     /locus_tag="ACHXOL_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR6810444.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5941..>6462)
                     /gene="fliD"
                     /locus_tag="ACHXOL_003558"
     CDS             complement(5941..>6462)
                     /gene="fliD"
                     /locus_tag="ACHXOL_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR6810445.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
BASE COUNT         1604 a         1697 c         1609 g         1552 t
ORIGIN      
        1 actccggttc acccttcagc atcaatggtc gggctggatg ctggcgtggc taaactcgcc
       61 acgctgtcag atggcacagt ctttgagcct gtaaacagtt ttcagaaaaa ccagaagaag
      121 ctggcgagac ttcagcgaca gttaagccgc aaggtcaaat tcagcaacaa ctggcagaag
      181 cagaaacgca aaatacagcg actgcattcc tgtatcgcaa atatccgcag ggactacctt
      241 cacaaagtca caacgaccgt cagcaaaaac cacgcaatga tagtcattga ggatttgaag
      301 gtcagcaaca tgtcaaagtc agcagcgggt acggtcagcc agccggggcg caatgtccgg
      361 gcaaaatcag gtttaaaccg ttcgatactg gatcagggct ggtatgaaat gcgccgccag
      421 cttgagtaca agcagctctg gcgtggcggt caggtgcttg ctgttccgcc agcgtacaca
      481 agccagcgtt gcgcgtgctg tggtcataca gcgaaagaaa accgcctgtc acaaagtaaa
      541 ttcagatgcc aggtatgtgg atatacagtg aacgccgatg taaatggcgc tcgtaacatt
      601 ttagcggcgg ggcacgccgt tcttgcctgt ggagagatgg tgcagtcagg ccgcccgttg
      661 aagcaggaac ccaccgaaat gattcaggcg acagcctgaa cgtagcaggg atcctcgtcc
      721 ttcagggcgt ggaggatgtc aacaaacagg ttgaattaac tccgcgccct ggtttttgac
      781 attacctacc gcgcgcgata ccgggtgcca ggtgaactgg tttgctggca cacagccgtt
      841 ggtagcaatt tctgatgctt ctttaccgcc aatctcttgc cgcatccatt cctgtgcagc
      901 ttctggcgac agaaccagcg ggcggcggtc atgaatgtct accagaccct ggtctgctgc
      961 agcagtgaca attaaaaatc cttcggcttc gtcaccacgc tcaaatggtg tgctacctat
     1021 cgcggccata aaaataggtt gtccatcagc acgatagata aaaaaaggct gttttttgtc
     1081 gccttctttt ttccactcaa accagccatc ggcaaaacag attgcccgac catgttgcca
     1141 gagcggttta aacatgcgac tggttgcggc agtttctacg cgggcgttaa tcagcggcgg
     1201 tttatcccac catccgggag catatcccca gaaaaccgga tccagatgaa ggtgttcatc
     1261 acgttcactg agcagaagaa ctttggttcc cggcgcgacg ttgtatctgc caatgggttc
     1321 gggatcgtag ggaatatcgc gttcaatatc ttccgcgaga agcgcaaggt aatcttcacg
     1381 cgtttgggat tgggcaaagc gtccacacat agaaacctcc tgagatcata ctgaaaagaa
     1441 taggagaggg ggcgggtaaa atctatgtgt cagaggcaac aatgttcccg ctgtgggcca
     1501 cagcgggaat gagggattac ttctgaatta aataacgaat ggtcggcccg tcttgctgaa
     1561 tatccagcac cgtatagccg tgattacgcg catccagtgg aatattattg atcgactgtg
     1621 gacagtcgct caccacttcc aggatttccc ctttttttaa ctgcggcatc gcctcaaggg
     1681 ttgcgactgc cggataaggg cagggttcac ccaccatatc aaggcggtaa tcaggaacga
     1741 tatttttcat gcgatctcct tagcagtctg cggtgccgca cggcggaaga agcgtttttc
     1801 ccagccgata atcaacatca gcgcagtaaa caacagcaaa tatgtcacca gcaggccacc
     1861 catcgggcca aaggttttca gcaggttgat tttgtcccag tcggtggcca gcgccggagc
     1921 gaaatcatcc cagtaatacg ccagaatcgt cgagccgatc acattgccca gaccgaccca
     1981 ccagtagtgc acctggcctt ctaccgcgcg gtacatccag ccggtttcgc agccgcctgc
     2041 cagcacgatg ccaaaaccaa acagtaaacc accaattacc gcgtttggtc ccgcccacat
     2101 gattttgggt tcaacgccta actgtacgta actgaagatc ccgatggcac tcaccgccat
     2161 accgataatg attgctttcg ccatatgggt acgtccggtg atccacatat cgcggaacgc
     2221 cgaagtaaag cagatttgcg cacgttcaat cagtaaacca aagccgacgc caaacagcat
     2281 tgccagcccc agtttgggtt ggttcatcgc cgtgagcagc gcccagccca gcatgccgaa
     2341 aaagaccagc atcccgagac gaaaacgacg ccgcgcctga tccggttttt gcgtcagcgg
     2401 tgaggcggca gaaactttct gcattttcac gggaatacgg aagatgggca gcagggtaaa
     2461 gcgcgcgcca aaccatgaac caatggcagt ggcgatggca aagaaccagg catgcagcga
     2521 gaactgagga atgccggtaa agaacgccgc caggttacag cccattgcca gacgcgcgcc
     2581 aaaaccggcg ataatgccgc caataatggc ctgcataatg cggatacggc tgcgcggcat
     2641 gcgcagtttg acattgttgg cccacagcgc tgcggcaaag cagccgccaa acatgccgag
     2701 gatcatcatc ccgtcgatgc gggttaatgg cgatccttcc agatggataa ttttaaaata
     2761 accccactct tcagcatgga cgccaaacag ctgcaggagc tgaccgcccc aacgggtaaa
     2821 ttcacccgtg acagcccaaa aggtgccagt aatgccaaaa tagtaagtgg agagaatacc
     2881 cgccgcgatg accgcaggga tgggtgccca gaatttaatc aaccaggcgt gtttgaattg
     2941 ctgccatgac atgaatgtag cctctgaact cagaaggttt gaaacaagaa tcgcgaatca
     3001 tacatcatgc aaatttattt tcggggtaat aattcacaaa aataatagtt agatcaaatt
     3061 tctctctgaa cctggaaagg ctggcgacat cagggcttta atgccacaat cctcttttct
     3121 tctcatgcag gacgaatgat gaaaaaactc gcaattgcag gtgcgctcat gttgctggcg
     3181 ggctgtgccg aagtcgaaaa ctataacaat gtcgtgaaaa cccccgcgcc ggactggctg
     3241 gcgggctact ggcagaccaa agggccgcaa cgtgcgctgg tcagtccgga agcgataggc
     3301 agcctgattg tcactaaaga gggcgatacc ctggattgcc gtcagtggca gcgggtgatt
     3361 gccgtgccgg gcaagttgac attgatgtct gatgatctca ctaacgttac cgtaaaacgt
     3421 gagctgtacg aggttgagcg tgacggtaac accattgagt acgatggtat gacgatggag
     3481 cgtgtggatc gcccgacggc agagtgtgcc gccgcgctgg ataaagcgcc gttaccgacg
     3541 ccactgccgt aatgaaatct ccccggcttg ccgccgggga tgaatttaaa tcacctcttc
     3601 gataacccac acgctgacgc tgccgccgtt gcaaaagaag gtcgcttcgc cgttttcgtc
     3661 ggtcactacg ctctcttgcc gattccccag aaaatcacgc caggttttat tgccgtaatt
     3721 ctctcccaga tgaatggttt tttcgccatc atccccgttc gacatgacca ccacgcagcc
     3781 gggatattcg tcagtgccac tgcggctaaa ggcaatgcag ttcggatggt cgaaaaataa
     3841 cgtctgtaca ccgtgggcga aacgctgacg ggcgagaatt aactcatcaa gctgttcgat
     3901 tattggcata tctatcggat aggtttgccc gtcaccaccg acatcttcgt aatgcgcacc
     3961 gtagaggtcc ggatagaaca ccgaaggaac gccattttcc cgcaacaaaa ttagagcata
     4021 cgccagcggt ttaaaccacg gttcgaccgg cgcttcgagg gcttgcaacg gttgggtgtc
     4081 gtgattggct acgagggtta cggcgtggaa aggatcggct tccactaatg tacccgtgaa
     4141 aatctgcgtc atgtcgtagt cgcgtcccat gcgcgatgct tcatggaatt tcatctgcag
     4201 cggcgcatcg aacagcatgg ttttgccttc cacctgatca atatacgttt gcagtttatc
     4261 gacttcatgc gaccagtact ctgccacaat aaacagcggc tttggcgcaa cttcctgtac
     4321 gtgttcgatc cactctttat aaaaccacgc cggaatatgt ttaaccgcat caagacgaaa
     4381 accgtcgcat tgtgtttgtt ccatcaccca gcgcgcccag tatttaatct cttccgtcac
     4441 ggcatgattg cggaaatcga tattctcacc catcagataa tcgaaattac cgagttcatc
     4501 atcaacctga tcgttccagc cttcgccggt gtagtcgtta acaattttaa atatgccatc
     4561 ttcatcagga ttttcgatat ggtcgatacc gctaaaacat ttgaagtccc agataaactg
     4621 cgagtattgc ccggcacggg cggggaaggt gtaacgcgtc cagccttcgc actcaatgat
     4681 ttcttcatca atttgcgtac ggtcatcagc atttacacgc tgcacgcgaa tagcttcttt
     4741 ttcatccgcc cccattttgt ggttgactac cacgtccagt agcaccgcaa tgtcattacg
     4801 tttcagagcg tcaatggcgg ccagcagttg tgctttatcg ccatatttag tagggatgct
     4861 gcctttctga tcaaactcac ctaaatcaaa taaatcatag gagtcgtagc cgaccgaata
     4921 cccgcccgat gcgcctttat aggcgggcgg caaccagacc atattgatac caatatcatt
     4981 aaaaccgtcg gcgcgctcgg ccagttcagg ccagagctta ccgccttccg ggtaatacca
     5041 gtgaaaacat tgtaacagcg tgggattacg cattcttcga ctccattcaa ggggaacatt
     5101 agaagcgtag ccgtaatcgg attattcgcg agccatcggc tcgttaagat tcaaaagagg
     5161 ttatcctgcg gagccagcac aaagccgccc tgatctccat aggcacttaa cactgatttt
     5221 tgcaccgatg actgaccgac cagtttcgcc agttcatcca tacgaatctg taataactgc
     5281 tttaccttgc tttcgttgtc gagaatcagg cgcagcatcg ggcggagctg ctcctgcatc
     5341 gtggtggacg gggcaacctc ttcagtcaga tgtgcaatct cctgcaccgc attcacatac
     5401 gccatttcgc tggcgatgag ttcgtcccat tgttcttccg ttgccaggcg taacatgagc
     5461 tggcttttat cgacgagttg ttgccaggcg aaatacaaat gcggtgcatg gttcatcaga
     5521 ctgggtcctg aatcaaagaa ggggagagta aagactcttt ccaggcatcg gcaatattgc
     5581 gcattaatgc ttccacttct tcgactgcgg agacatcgtt gcgtaaattg gcttgcagca
     5641 agcgcctgac catatagcta taaagagcaa tcaagttttg ggttagttcg tctttgctct
     5701 cttcatcaag actcacccgc agcccgttct caatgatgtt gatcgctttt gacaaagaga
     5761 cgcctttgcc ttgctgattg ttgtcctgca taaacaggcg cgctctaacc agtgcgctca
     5821 gcactccatc aaatagcatg gtgaccagct gttgctggct ggcgctcatt acggcgcttt
     5881 cgacgccaat ttgtgcatag gcttttgagc cgctcgcggt atacatgctg acctccgtga
     5941 tcaggaatta ctcattgcag taaattgttg gctgagatag gtactggtgt tattcagttt
     6001 gctcatcatg gtatctaatt gcgtaaactg tgcagtgtaa cgcgccacgg tgtcatcaat
     6061 gctggcactg acggatagat actgtttggt cagttttttc agtgtggcgt taatactgtc
     6121 ctgtgcgcta tcaataatgc catcatcggc aaggtaacct tttacctcgg tggcaatttt
     6181 ggtggtaatt cctgtttctt taccatcgcc taccagcaat tcacgtacgg atgctgtatt
     6241 ttcattcagg acttttttga gtttatcatc atcaattttc agcttacccg tcgtgccgtc
     6301 ctgcttaatc ccgatctcgt ttaatgtttt gaaggcactg tcgctggcac cgtttgcaaa
     6361 ttgtgcgcgg atgccgcttt gaattgtacg tacaacgctg tctccgagaa gtgcaccatt
     6421 atttttatct tgtgcttcag cacctgggtc tacttcttta ta
//
LOCUS       DAZRUF010000224         6337 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZRUF010000224 DAZRUF010000000
VERSION     DAZRUF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.225"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHXOL_003559"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHXOL_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR6810446.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHXOL_003560"
     CDS             2219..2767
                     /locus_tag="ACHXOL_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR6810447.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHXOL_003561"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHXOL_003561"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR6810448.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHXOL_003562"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHXOL_003562"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR6810449.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHXOL_003563"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHXOL_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR6810450.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1592 a         1557 c         1607 g         1581 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcg tcaataagtt
     6181 ctgtcaataa aaatttacgg aactattgat gagagtttgg tgtctttatg tgtctgcagg
     6241 catctttcca ttcaaactaa cgtttcgcta ccgtgaaagt gctacaaaga taaagattta
     6301 gtttcaaaaa gaaaaaatat gtaaccaaaa gtaaaaa
//
LOCUS       DAZRUF010000225         6285 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZRUF010000225 DAZRUF010000000
VERSION     DAZRUF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.226"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..357
                     /gene="mcbR"
                     /locus_tag="ACHXOL_003564"
     CDS             <1..357
                     /gene="mcbR"
                     /locus_tag="ACHXOL_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR6810451.1"
                     /translation="LTPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSN
                     MPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLA
                     EIMQQNIAILYQQYNR"
     gene            complement(393..2495)
                     /gene="pqqU"
                     /locus_tag="ACHXOL_003565"
     CDS             complement(393..2495)
                     /gene="pqqU"
                     /locus_tag="ACHXOL_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR6810452.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDIP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAARRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2737..3798
                     /locus_tag="ACHXOL_003566"
     CDS             2737..3798
                     /locus_tag="ACHXOL_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR6810453.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFYTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3913..5412)
                     /gene="ansP"
                     /locus_tag="ACHXOL_003567"
     CDS             complement(3913..5412)
                     /gene="ansP"
                     /locus_tag="ACHXOL_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR6810454.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGMPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5679..>6285
                     /locus_tag="ACHXOL_003568"
     CDS             5679..>6285
                     /locus_tag="ACHXOL_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR6810455.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGAGHEWFQDNATNISAIADAVCQLPKLQEVLKRN"
BASE COUNT         1691 a         1592 c         1503 g         1499 t
ORIGIN      
        1 ctcaccccgc aagacctgac ggaactccag gaattgctcg agaaattaca gcaggcgcaa
       61 gaaaagggcg acatggaaca aatcattaat gtaaacaggc tatttcgctt agcgatttat
      121 catcgctcaa atatgcccat cctgtgtgag atgattgagc aactgtgggt caggatgggg
      181 cctggtttac attatctcta tgaagcgatt aatccagcgg aattacggga gcatatagaa
      241 aactatcatc tgttactcgc tgcgttaaaa gcaaaagaca aagagggatg cagacattgt
      301 cttgctgaaa ttatgcaaca aaatattgct attttatacc agcaatacaa tcgttaaagt
      361 aaatgcccgg caaataccgg gcatcaaggt aattactcaa atctccacgc aatattcatg
      421 ccgacaccat aatttcgtcc gggcgaaggt tcgtaatatc gcccgtttga ctcattgaca
      481 atgacagaac caacgtattc tttatcgaat aaattatcga cacgaccaaa taaatccaca
      541 gttaaattgt ggtaattgta tttatacccg gtgaataagc cgacgagagt ataagacggc
      601 gcttttgccg tattttcatc atctgccata atatcgccca tataacgcgc ttccgtgcct
      661 gcataccaac catcttccgg tacataacct atcgacgcaa agcccatatt acgggcgatc
      721 cctggcatcc gattaccgtt acaatcctgt tcattgcaaa cattgctgcg ataggtcgca
      781 tcaagccagg tccaggacgc gtttacgcga aaatctcctg cgaaacgttg atcccatgcc
      841 agttcagcgc cttgacgacg ggtctttccg gcatttttgt aagtcgtacg cccaccgcta
      901 ctgctatcga caacaatttc atcatcagtg tcggtctgaa acaatgcgag actaagcagc
      961 ccatcaccaa tacgcgtttt actgccgatc tcaattgtat cgttggtgga tggttttaaa
     1021 ccaaagttca taccgctttg cccatcagca cgataagaca gctcattaat cgtcggcgtt
     1081 tcaaaacctc gcctggctgc cagatagata ttccaggcat cggtcattgc atattttaac
     1141 gaaccggcag gtagccattt atgataactg gcatcaccgc tgtcatcgcc gttacccgga
     1201 gtaacgtaat ggtcgttgga atcaaaccac acggagctgt agcgcacgcc agcatccagc
     1261 gacagttttt cgctcagctg ccactgcgtc tgtaaatagg gatcgatgtt ccacatcaga
     1321 ttgcgttcgt cgcgacgcaa ctcacctttt tgcccgtact ccggcatgcc gctattcagg
     1381 cggaagttat tgtagccctt gcggttttca ctcatgtttt cgtagttcag gccggtagtg
     1441 aacgtgaccg gaacgcccag ttcaccacgg tgtgtccagc ggctgtctat cccctggtaa
     1501 tggcgttgca gggtaatcac gccgcccgca tgtgacgggt taagttgtgg tgccatgggt
     1561 attgactggt actgggtcgt ttctcgctct ccggcataca tcatcacact catatcatct
     1621 tgcgcgctca ggctacgctc atagcgcaac ccagcctgag tttgcttgat ggtttttcgc
     1681 gtgtcgtact gttctgcacg aggcgcttgt tgtggattag ccttccattc tgctttggtt
     1741 agcccacccg ggtcatctgc tttgatatcc acactattga aaatcagact taatttgctg
     1801 gcttcatcaa tgcgtacgcc cagtttggca ttggctaaat ttttctgtgc gccactatgg
     1861 tcacgatagc cgtgggtcgt aaaacgcgtg gttgagacgg tgtaatcgac atcgccaggc
     1921 tgtgtgccgt ctcccgttgc gcccgttgct ttcagcccat agcgccagct gccaaaactg
     1981 ccgtagtaac tactggcttc aatggttggt ggctgttgtc cggtctgggt ggtgacattc
     2041 attaccccac cagacgcgtt gccatacagg gcagagaagg ggccacgcag cacttccaca
     2101 ttttgcacac tgcttaaatc gatgttggat gtttgccctt gcccgtcggg catggtggcg
     2161 ggaataccgt ccacatacag gcgaataccg cgaataccgt aagtggagcg ggagccaaat
     2221 ccgcgaatcg acagctgtaa atcttgcgca tagttctgcc ggttttgtac ctgcaaacca
     2281 ggcacgtcgg tcagtgattc ggacaagtta atgcgcggtg ttgccaggcg catctcctcg
     2341 ccatccacca cgcttactgc tgctgggata tccagttctg aaaccacctg cggtgcggca
     2401 ctgacaatca tggtctgttc atcagcggca aaaacaacgg gggaaagggc aagtagtgcg
     2461 ggcaaaacgg tctgtcggac ggaaaaaatc ttcatgaaaa aagccaggtt aagaatggga
     2521 aaacgccgtt atggtaataa aattgtaaat tcatggaaaa cgaaacggca cgatacgtta
     2581 agtaattgag aaaattgtag tcgtaacggc aagaaatgct ccacatttga gaaaataatg
     2641 attaccattc ccatttataa caagagcgta acgatgatta cgcttagcga agcattgtga
     2701 agcagcaaaa atatcggttc atcaaaggga gtcgtcatgc atttacgtca tctgttttca
     2761 tcgcgcctgc gtggttcatt actgttaggt tcattgcttg ttgcttcatc attcagtacg
     2821 caggccgcag aagaaatgct gcgtaaagcg gtaggtaaag gtgcctacga aatggcttat
     2881 agccagcaag aaaacgcgct gtggctcgcc acttcgcaaa gccgcaaact ggataaaggc
     2941 ggcgtggttt atcgtcttga tccggtcact ctggaagtga cgcaggcgat ccataacgat
     3001 ctcaagccgt ttggtgtcac catcaataac acgactcaga cactgtggtt tggtaacact
     3061 gtaaacagcg cggtcacggc gatagatgcc aaaacgggcg aggtgaaagg ccgtctggtg
     3121 ctggatgatc gtaagcgtac ggaagaggtg cgcccgctgc aaccgcgtga gctggtagct
     3181 gacgatgcca cgaacaccgt ttacatcagt ggtattggta aagagagcgt gatttgggtc
     3241 gttgatggcg agaatatcaa actgaaaacc gccatccaga acaccggtaa aatgagtacc
     3301 ggtctggcgc ttgatagcaa aggcaaacgt ctttacacca ctaacgctga cggcgaattg
     3361 attaccatcg acaccgccga caataaaatc ctcagccgta aaaagctgct ggatgacggc
     3421 aaagagcact tctttatcaa cattagcctt gataccgcca ggcagcgtgc atttatcacc
     3481 gattctaaag ccgcagaagt gttagtggtc gatacccgta atggcaatat tctggcgaag
     3541 gttgcagcac cggaatcact ggctgtgctg tttaacccag cgcgtaatga agcctacgta
     3601 acgcatcgtc aggcaggtaa agtcagtgtg attgacgcga aaagctataa agtagtgaaa
     3661 acgttctata cgccgactca tccaaacagc ctggcgctgt ctgccgatgg caaaacgctg
     3721 tatgtcagtg tgaaacaaaa atccactaaa cagcaggaag ctacccagcc agacgatgtg
     3781 attcgtattg cgctgtaata gatatggcga gggtgaaaac ggagagactt gcctgacttt
     3841 tatcacgccg catgcggcac accttcgtca gtaatctaaa tttgcccggc acgtagccgg
     3901 gcaattttgc aattagctcg ccgtttcagg cttaaacaca atttcctgtt tttcttcatc
     3961 ttcctcgacg actggcgcag tgctgtgaat ttcagcaacg cgtttgcgca caccaaacca
     4021 gcctataacc agcagaatac cgataatcgg cagcgccgcg atagtataag tcccgttcgg
     4081 gtaatcgaac gccatcagca caaggacact cagtaaaaac agtaatgtca gccaggaagt
     4141 gaagggcgcg ccaggcagtt taaaactgac atccgctgct ttgccttctt taatcgcttt
     4201 acgcaggcgc atctggcaca cgatgataaa cgcccatgaa gcgatgattc ccagcgacgc
     4261 gaagttcaac acaatctcaa atacgcgcga cggcaccaga tagttgagga atacgccgac
     4321 gacatacaca accagtgtcg ccagaatccc ggcatacggc acatgctgac gactcatttt
     4381 cgccataaaa ctcggtgcgg aaccgcccat cgccatcgag cgcagaatac gcccggtgca
     4441 gtacagacct gagttcaggc tggagagggc agcggtcagc accacaatgt tcataatgct
     4501 gccgatatat ggcataccca gtttagagaa aaacgtcacg aacggacttt gccccgcctg
     4561 atacgcgctc cacggcaata acataaccag caacaccacg gagccgacgt aaaacaggcc
     4621 aatacgccaa atcacactgt taatggcttt aggcaccatg gtctgcggat ctttacattc
     4681 tccggcagct gtacccacca tttcaatgga ggcaaaagca aacactacgc cctgaatcaa
     4741 caccagcgca ggcagcaaac cgtgggggaa gaagccgcca ttatcggtga ttaaatgaaa
     4801 gccagtggtg ttgccatcca gcggctgacc actaccgagg aacactgtac ccacgaccaa
     4861 aaaggtcaca atggcgagca ctttaataag cgcaaaccag aactccatct ccgcaaacca
     4921 tttcacaccg atcatattca tggtgccaac gatggtaagt gcagcgagcg caaagaccca
     4981 ctgcggcacg ccgccaaacg caccccagta atgcatatac agagcgacgg cggtaatatc
     5041 aacaatcccc gtcatcgccc agttgatgaa gtacatccag ccagcaacat aagcggcttt
     5101 ctcacccaaa aactcacggg cataagagac aaaactgcca ctggaagggc ggtgtagcac
     5161 cagctcaccc aatgcacgca gaataaaaaa cgaaaacaag ccacaaatta aataaaccag
     5221 tgccagtgct ggacccgcca tttgcagtcg ggctcctgca cctaaaaaca agccggtgcc
     5281 aatcgcgccg ccaatggcga tcatctgtac ctggcgattg cccatcgctt tgtgataccc
     5341 ctcttcgtgg gcattaagcc agcggcgttt cgcggcgtgt tgatctgaag tgtcggtgtc
     5401 gtgtttactc attgctctcc ctgattgctt taatgaaaaa gtcatataag ttgccatgaa
     5461 caatgtttat tcatcctggc gatgatagtt atcaccatta ttctttatgg gtataaaact
     5521 atcggcgcag catcctaccc gttatcgata tacgatgcaa aacatcccct tacaatcctg
     5581 aaggggattc atacaactga cgaaaaaatg acaaatactt ttgctggtta acctgtgtac
     5641 tgtcctacac ttaatcttta aaagattgtg aggggcatat gattaaggtg tatggcgtac
     5701 ccggatgggg ctctacaatc agtgagctga tgctaacact ggctgatatt ccttatcagt
     5761 tcgttgatgt cagtggtttt gaccacgagg gaacttcacg cgagttattg aaaaccctga
     5821 acccgttgtg tcagattccg acactggcgc tggaaaatga cgagattatg acggagacag
     5881 cggcgattgc attgatggtt ctcgatcgtc gcccggacct tgcgccaccg gttgggcgcg
     5941 ccgaacgtca gcagtttcaa cggctattgg tctggctggt tgctaatgtc tatccaacat
     6001 tcactttcgc cgattaccct gagcgttggg ctcctgacgc acctgaacag ttaaagaaga
     6061 atgttattga atatcgtaaa tcgctttata tctggctgaa ttcacaattg accgctgaac
     6121 cttatgcgtt tggtgagcaa ctaacgctgg tggactgtta tctttgcact atgcgcacat
     6181 ggggggctgg gcatgaatgg tttcaggata atgccacgaa tatcagtgcg attgctgatg
     6241 ctgtatgtca gttaccgaaa ttacaagaag tattaaaaag gaatg
//
LOCUS       DAZRUF010000226         6258 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZRUF010000226 DAZRUF010000000
VERSION     DAZRUF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.227"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            50..979
                     /gene="pfkB"
                     /locus_tag="ACHXOL_003569"
     CDS             50..979
                     /gene="pfkB"
                     /locus_tag="ACHXOL_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR6810456.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1080..1370
                     /gene="ghoS"
                     /locus_tag="ACHXOL_003570"
     CDS             1080..1370
                     /gene="ghoS"
                     /locus_tag="ACHXOL_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR6810457.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1476..2336
                     /locus_tag="ACHXOL_003571"
     CDS             1476..2336
                     /locus_tag="ACHXOL_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR6810458.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2377..2913)
                     /locus_tag="ACHXOL_003572"
     CDS             complement(2377..2913)
                     /locus_tag="ACHXOL_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR6810459.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3060..3728
                     /gene="hxpB"
                     /locus_tag="ACHXOL_003573"
     CDS             3060..3728
                     /gene="hxpB"
                     /locus_tag="ACHXOL_003573"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR6810460.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3891..4481
                     /locus_tag="ACHXOL_003574"
     CDS             3891..4481
                     /locus_tag="ACHXOL_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR6810461.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4614..6005
                     /gene="tcyP"
                     /locus_tag="ACHXOL_003575"
     CDS             4614..6005
                     /gene="tcyP"
                     /locus_tag="ACHXOL_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR6810462.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6009..>6258)
                     /gene="ydjO"
                     /locus_tag="ACHXOL_003576"
     CDS             complement(6009..>6258)
                     /gene="ydjO"
                     /locus_tag="ACHXOL_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFR6810463.1"
                     /translation="PGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDK
                     LDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1526 a         1545 c         1574 g         1613 t
ORIGIN      
        1 ctttccgctg attcggtgcc agactgaaat cagcctatag gaggaaatga tggtacgtat
       61 ctatacgttg acacttgcgc cctctctcga tagcgcaaca attaccccgc aaatttatcc
      121 cgaaggaaaa ctgcgctgta ccgcaccggt gttcgaaccc gggggcggcg gcatcaacgt
      181 cgcccgcgcc attgcccatc ttggaggcag tgccacagcg atcttcccgg cgggtggcgc
      241 gaccggcgaa cacctggttt cactgttggc ggatgaaaat gtccccgtcg ctactgtaga
      301 agccaaagac tggacccggc agaatttaca tgtacatgtg gaagcaagcg gtgagcagta
      361 tcgttttgtt atgccaggcg cggcattaaa tgaagatgag tttcgccagc ttgaagagca
      421 agttctggaa attgaatccg gggccatcct ggtcataagc ggaagcctgc cgccaggtgt
      481 gaagctggaa aaattaaccc aactgatttc cgcttcgcaa aaacaaggga tccgctgcat
      541 cgtcgacagt tctggcgaag cgttaagtgc agcactggca attggtaaca tcgagttggt
      601 taagcctaac caaaaagaac tcagtgcgct ggtgaatcgc gaactcaccc agccggacga
      661 tgtccgcaaa gccgcgcagg aaatcgttaa tagcggcaag gccaaacggg ttgtcgtttc
      721 ccttggtcca caaggagcgc tgggtgttga tagtgaaaac tgcattcagg tggtgccacc
      781 accggtgaaa agccagagta ccgttggcgc tggtgacagc atggtcggcg cgatgacact
      841 gaaactggca gaaaatgcct ctcttgaaga gatggtccgt tttggcgtag ctgcggggag
      901 tgcagccaca ctcaatcagg gaacacgtct gtgctcccat gacgatacgc aaaaaattta
      961 cgcttacctt tcccgctaac aaaaacattc ccccagcatt gggggaatca ccaccaacct
     1021 gtcggcaacg cgtttctccg actttgctca aaagtcatgt gataacaaag gggtgaacta
     1081 tggccagtgg cgatcttgtc cgttatgtca taaccgtcat gttgcatgag gatacattga
     1141 ctgaaattaa cgagttgaat aattacctga ctcgcgacgg ttttttgctc accatgacgg
     1201 atgatgaggg aaatatccat gagctgggga ctaacacttt tggacttatc agtacccaaa
     1261 gtgaagaaga aattagagaa ctggtttcgg ggcttaccca aagtgcaacc ggcaaagatc
     1321 ctgaaatcac catcacgact tgggaggaat ggaatagcaa cagaaaataa atggtttttg
     1381 ggcaataatc agtctgtggt gtgcgttagc tcgtgttttt acaccgcatt cttgcgctaa
     1441 ccttatgatc tggcagacaa catgggagag acatcatgtg gcaggcaatc agtcgtcttt
     1501 tgagcgagca gttaggtgaa ggcgaaatcg aactgcgtaa tgaactgcct ggcggagaag
     1561 tccatgccgc atggcatttg cgctatgcag gacatgactt tttcgtcaaa tgtgatgaaa
     1621 gggaactgct tcccggtttt accgccgaag ccgaccaact ggagttactg tcgcgtagta
     1681 aaaccgtcac cgtgcctaag gtttgggcag taggcgctga ccgtgactac agttttctgg
     1741 tgatggatta tctcccacct cgtccgctgg atgcgcatag cgcatttatt cttggtcagc
     1801 aaattgcgcg tttacatcaa tggagtgacc aaccacaatt tggcctcgat tttgataact
     1861 cgctctccac aactccacag cccaacacct ggcaacgtcg ctggtcaacg ttttttgctg
     1921 aacaacggat tggctggcag ttggaactgg cagcagagaa agggatcgct ttcggcaata
     1981 tcgacgccat cgtcgagcat attcagcagc gtctggcctc gcatcaactg cagccttctc
     2041 tgttgcacgg cgatttatgg tccggcaact gtgcgctggg tccggatggc ccgtacattt
     2101 tcgaccctgc ctgctactgg ggtgaccgag agtgcgacct ggcgatgtta ccgctgcata
     2161 ctgaacagcc gccacaaatc tatgacggct atcagtcagt atccccgcta cctgccgatt
     2221 tccttgaacg tcaaccggtt taccaactct acacgctgtt aaatcgtgca agattatttg
     2281 gcggtcagca tttggttatt gctcagcagt cattggatag attattagca gcatgatatg
     2341 ggttgaggat aatggccgct ccgtgcggcc ttttgattaa ataaacccaa gcagagaaaa
     2401 gaacacataa cctgcagcaa tacaaataac cggcaggata tatagtgaga aaaactgcag
     2461 gaaaatagta tggtgcggaa caacaatacg ggactcaatt tgttcacgcg ttagcccctc
     2521 ttcccctttg gctttttcca gaatgagttg atcttcaaca ccttcccgca agaagcgcgc
     2581 ctggcgactc atccgggcac cggaatcctg caacgccagt ccgacaaaaa tcaggatgaa
     2641 aatcacccag aacataacgt tcacgccacc attaaaatta ggggtcgggg agttatacca
     2701 gaacagattc aaaaaaggcg tattcgcctg catcatatcg atcatgacat gagtaaagtc
     2761 gagcatgacc gcattaatgc cttcctggtt ttcctgccgg gtattcataa atttcagcaa
     2821 agaaatcaat gtggagatca atgcaggtat aaaaatcacc caacccaaaa tccttttcaa
     2881 aacagcaatg cgtccagctt gttgatacgt catgagttct ccttgattaa gacgcgtcgt
     2941 ttcacttagt ttacctgtag atatctgttt tcgcccattc tttaaaggcg atatgatagg
     3001 cgcttaatca taagcacggc ttaatacctt acacataatg ctctaaagga gaggttgtaa
     3061 tgtcaacccc gcgtcagatt cttgctgcaa tttttgatat ggatggatta cttatcgact
     3121 cagaaccttt atgggatcga gccgaactgg atgtgatggc aagcctgggg gtggatatct
     3181 cccgtcgtaa cgagctgccg gacaccttag gtttacgcat cgatatggtg gtcgatcttt
     3241 ggtacgcccg gcaaccgtgg aatgggccaa gccgtcagga agtagtagaa cgggttattg
     3301 cccgtgccat ttcactggtt gaagagacac gtccattatt accaggcgtg cgcgaagctg
     3361 ttgcgttatg caaagaacaa ggtttattgg tgggactggc ctccgcgtca ccactacata
     3421 tgctggaaaa agtgttgacc atgtttgact tacgcgacag tttcgatgcc ctcgcctcgg
     3481 ctgaaaaact gccttacagc aagccgcatc cgcaagtata tctcgactgc gcagcaaaac
     3541 tgggcgttga cccactgacc tgcgtggcgc tggaagactc ggtaaatggc atgatcgctt
     3601 ctaaagcagc ccgcatgcgt tccatcgtcg ttcctgcgcc agaagcgcaa aatgatccac
     3661 gttttgtatt agcaaacgtc aaactctcat cgctgacaga actcaccgca aaagaccttc
     3721 tcggttaatg accaggggca gtgatcacgc tgcccctggt tctttatctg aatttgcatt
     3781 caactgacgg attaatcgtc aatttaagag aaagagttac accgtcacca cttccgtgca
     3841 ctgtataaaa atcctatact gtacgtatcg acagtttagt gagttttatc atgacggcgg
     3901 aaggtcacct tctcttttct attgcttgtg cggtatttgc caaaaatgcc gagctgacgc
     3961 ccgtgctggc acagggtgac tggtggcata ttgtcccttc cgcaatcctg acgtgtttgt
     4021 taccggacat cgatcaccca aagtcgtttc ttgggcagcg attaaaatgg atatcaaaac
     4081 cgatcgcccg cgcttttggg catcgtggtt ttacccacag tctgctggcg gtatttgcgc
     4141 tgctggcaac cttttacctt aaggttccgg aaggctggtt cattccggct gatgcgctac
     4201 aaggaatggt gctgggttat ttgagccaca tacttgccga tatgctgaca cccgccggtg
     4261 ttcccctgct ctggccatgc cgctggcgtt tccgcttgcc tatcctggtt ccccaaaagg
     4321 gcaaccaact ggaacgtttt atctgcatgg cattatttgt ctggtcggta tggatgcccc
     4381 attcattacc cgagaacagc gctgttcgtt ggtcatcgca aatgatcaat accttgcaga
     4441 tccagtttca tcggcttatt aagcatcagg ttgaatacta aaaaggcaaa aatcaccttt
     4501 ctggaataag caattccatt tgaatataag agccagctca cagttctgtt aatcttgcgc
     4561 caacactatg actgctacgc agtgatagaa ataataagat caggagaacg gggatgaact
     4621 ttccattaat tgcgaacatc gtggtgttcg ttgtactgct gtttgcgctg gctcagaccc
     4681 gccataaaca gtggagtctg gcgaaaaaag tgctggtggg tctggtgatg ggtgtggttt
     4741 ttggccttgc cctgcatacc atttatggtt ctgacagcca ggtacttaaa gattctgtac
     4801 agtggtttaa catcgttggt aacggctatg ttcaactgct gcaaatgatc gttatgccgt
     4861 tagtcttcgc ctctattctg agcgcggttg cccgtctgca taacgcatct cagttaggca
     4921 aaatcagttt tctgaccatc ggtacgcttt tgtttaccac gctgattgcg gcgctggtcg
     4981 gtgtgctggt caccaacctg tttggtttga cggctgaagg tctggttcag ggtggtgcag
     5041 aaactgcacg tctgaacgcc attgaaagta actatgttgg taaagtctct gatctgagcg
     5101 ttccgcagct ggtcttgtcc tttatcccga aaaacccgtt tgccgatctt accggagcca
     5161 atccgacgtc aattatcagc gtggtaattt ttgccgcatt cctcggcgta gctgcattga
     5221 aactgctgaa ggatgatgcg ccgaaaggtg aacgcgtctt agccgctatc gataccctac
     5281 aaagctgggt gatgaaactg gttcgccttg tcatgcagtt gaccccttac ggcgttctgg
     5341 ctctaatgac caaagtggtt gcaggttcta acttgcaaga catcatcaaa ctgggaagtt
     5401 tcgttgtcgc gtcctacctc ggtctgctga ttatgtttgc agtgcatggc attctgctgg
     5461 gcattaatgg cgtgagtccg ctgaagtact tccgtaaggt atggcctgtg ctgacgtttg
     5521 cctttaccag ccgttccagt gctgcgtcta tcccactgaa tgtggaagca caaacgcgtc
     5581 gtctgggcgt tcctgaatcc atcgccagtt tcgccgcctc tttcggtgca accattggtc
     5641 agaacggctg cgccggtttg tatccggcaa tgctggcggt gatggttgcg cctacggttg
     5701 gcattaaccc gctggacccg atgtggattg cgacgctggt cggtattgtt accgttagtt
     5761 ccgcaggcgt tgccggtgtc ggtggtggtg caactttcgc cgcactgatt gtgctgcctg
     5821 cgatgggcct gccagtaacc ctggtggcgc tgttaatctc cgttgaaccg cttatcgaca
     5881 tgggccgtac ggcgctaaac gttagtggct cgatgacagc tggcacgctg accagccagt
     5941 ggctgaagca aaccgataaa gccattctgg atagcgaaga cgacgccgaa ctggcacacc
     6001 gttaattctt atgctggcaa cggtccgttt tgtatagggg ccgttgcttt actttttaaa
     6061 tgttccatcg cggcgatgca tactgactca tatttaaaca gatcgtcagc gaaaaaaatg
     6121 tccaacttat caatacattc ctggctcaat gaatgtgcct gtagtgagaa gcttaaaacc
     6181 tcatacccga gacgttttct aagtgaacta tctggaatac tcataatatc ttcaagcaca
     6241 ccacttccca ttcccgga
//
LOCUS       DAZRUF010000227         6237 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZRUF010000227 DAZRUF010000000
VERSION     DAZRUF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.228"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            132..1352
                     /gene="astC"
                     /locus_tag="ACHXOL_003577"
     CDS             132..1352
                     /gene="astC"
                     /locus_tag="ACHXOL_003577"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR6810464.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            1349..2383
                     /gene="astA"
                     /locus_tag="ACHXOL_003578"
     CDS             1349..2383
                     /gene="astA"
                     /locus_tag="ACHXOL_003578"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR6810465.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     IYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            2380..3858
                     /gene="astD"
                     /locus_tag="ACHXOL_003579"
                     /pseudo
     CDS             2380..3858
                     /gene="astD"
                     /locus_tag="ACHXOL_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            3855..5198
                     /gene="astB"
                     /locus_tag="ACHXOL_003580"
     CDS             3855..5198
                     /gene="astB"
                     /locus_tag="ACHXOL_003580"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR6810466.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            5191..6159
                     /gene="astE"
                     /locus_tag="ACHXOL_003581"
     CDS             5191..6159
                     /gene="astE"
                     /locus_tag="ACHXOL_003581"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR6810467.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         1346 a         1584 c         1856 g         1451 t
ORIGIN      
        1 atttccagtt agcctccgcc gtttatgcac ttttatcact ggctggcacg aaccctgcaa
       61 tctacattta cagcgcaaac attacttatt gttaacatat agatagcgaa ttatttactg
      121 tagaggtcgc tatgtctcag ccaattacgc gtgaaaactt tgatgaatgg atgatacctg
      181 tttacgctcc ggcacccttt ataccggtac gtggcgaagg ttcgcgcttg tgggatcagc
      241 aggggaaaga gtatatcgac ttcgcgggtg gcattgcggt gaacgcgctg ggccatgcgc
      301 atccggaact gcgtgaagcg ctgaacgaac aggcgagtaa gttctggcat accggcaacg
      361 gttacaccaa cgagccggta ctgcgactgg cgaaaaaatt gatcgacgcc acgtttgccg
      421 atcgcgtctt cttttgtaac tccggtgcgg aagccaacga agcggcgcta aaactggcgc
      481 gtaaattcgc tcacgaccgc tacggcagcc ataagagcgg tatcgtggcg tttaaaaatg
      541 cgtttcatgg tcgcacgctg tttactgtca gtgcgggtgg gcagccagcc tattcacagg
      601 attttgcgcc actgccacca gatatccgtc atgctgcata taacgatatt aactctgcca
      661 gcgcgctgat tgacgacgcc acctgtgcgg tgattgtcga acccatccag ggggaaggcg
      721 gtgtggtgcc agccagcaac gcgtttttac aaggtctgcg tgaattgtgt gaccgccaca
      781 atgcgctgtt gatttttgat gaagtacaaa ccggcgtcgg gcgcaccggg gaactgtatg
      841 cctatatgca ctacggcgtg acacctgatc tgttaactac cgccaaagcg ctgggcggcg
      901 gtttcccggt cggtgcgttg ttaacaaccg aagagtgcgc cagcgtgatg accgttggca
      961 ctcatggcac cacctatggc ggcaatccgc tggcctcggc ggtggcgggc aaagtgctgg
     1021 agctcatcaa cacaccagag atgcttaatg gcgttaaaca gcgtcacgac tggtttgttg
     1081 agcgtcttaa taccattaat caccgctacg gtttgttcag tgaagttcgc ggcttaggtt
     1141 tgttgattgg ctgtgtactg aatgccgatt acgccggaca agcgaaacag atctctcagg
     1201 aggcggcgaa agcaggcgtt atggtactga ttgcgggtgg caacgtggtg cgttttgcgc
     1261 ctgcgctcaa tgtcagcgaa gaagaggtga cgaccggact ggatcgcttt gcagctgctt
     1321 gcgaacactt tgttagccga ggttcatcat gatggtcatc cgtcccgttg agcgctcaga
     1381 tgtctcggcg ctgatgcagc ttgccagcaa aacgggcggc ggcctgacgt cgcttcccgc
     1441 caatgaagcc acgctttccg cgcgtatcga aagggcaatc aaaacctggc aaggcgaact
     1501 gcccaaaagc gagcagggct atgtgttcgt gctggaagat agcgagacag gcaccgtggc
     1561 ggggatttgc gccattgagg tggcggttgg gctgaacgat ccctggtaca actatcgggt
     1621 cggcacgttg gttcacgcct caaaagagct gaatatctat aacgcattgc cgacgctgtt
     1681 tcttagtaac gatcacaccg gcagcagcga gctgtgcacg ctgtttctcg acccggactg
     1741 gcgcaaagag ggcaacggct atttgctgtc gaaatcgcgc tttatgttta tggcggcttt
     1801 tcgcgacaag tttaatgaca aagtggttgc tgaaatgcgc ggggtgattg acgaacacgg
     1861 ctattcaccg ttctggcaaa gcctcggtaa acgcttcttt tcgatggatt ttagccgcgt
     1921 cgattttctc tgcggcaccg ggcaaaaggc atttattgca gaactgatgc cgaaacatcc
     1981 gatctatacc cactttttat cccaggaagc ccaggacgtc atcggtcagg tacatccgca
     2041 aaccgcgcct gcccgcgcgg tgctggagaa agaaggtttt cgttaccgca actatatcga
     2101 catcttcgac ggcgggccaa cgcttgagtg tgacatcgac cgcgtgcgcg ccatccgtaa
     2161 aagtcggctg gtggaagtgg cagaagggca gcctgcgcag ggcgattttc cggcctgcct
     2221 ggtcgccaat gaaaattatc accatttccg cgtggtgctg gcgcgtaccg atccggcaac
     2281 cgagcgtttg attttaaccg ccgcacaact ggatgccctc aaatgccacg caggggatcg
     2341 cgttcgtctg gtgcgcctgt gcgcagagga gaaaacagca tgactttatg gattaacggt
     2401 gactggataa cgggccaggg cgcatcgcgt gtgaagcgta atccggtatc gggcgaggtg
     2461 ttatggcaag gcaatgatgc cgatgccgct caggtcgaac aggcttgtcg ggcagcccgt
     2521 gcggcgtttc cgcgctgggc gcggctctca ttggctgaac gtcaggtcgt tgtcgaacgc
     2581 tttgccggat tgctggaaag caataaagcc gaattaaccg cgattattgc cagagaaacg
     2641 ggtaagccgc gctgggaagc ggcaaccgaa gtgacggcga tgatcaataa aatcgcgata
     2701 tcaattaagg cgtatcacgt tcgtaccggc gagcagcgta gtgaaatgcc ggacggtgcg
     2761 gcgagcctgc gacatcgccc gcacggcgtg ctggcggtgt ttggggcgta taatttccct
     2821 ggtcatttgc cgaacggaca tatcgttccg gcattgctgg caggtaacac cattatcttt
     2881 aaacccagcg aactgacacc gtggagtggc gaagcggtaa tgcgtttatg gcagcaggct
     2941 ggcttgccgc cgggcgtgct gaacctggtg cagggcgggc gtgaaacggg tcaggcgctg
     3001 agtgcgctgg aggatctcga cggtttgctg tttaccggta gcgccaatac cggctaccag
     3061 ctgcatcgcc agctctccgg tcagccggag aaaattctcg cccttgagat gggcggtaat
     3121 aatccgctaa ttatcgatga ggtggcggat atcgactcgg ctgtccatct gaccattcag
     3181 tcggcgtttg tcacagccgg gcaacgctgc acctgcgccc gccgtttatt gctcaaaagc
     3241 ggagcacagg gcgatgcgtt tcttgctcgt ctggttgccg tcagccagcg attaacgccg
     3301 ggcaactggg atgacgaacc gcagccgttt attggcgggc tgatttctga acaggccgca
     3361 cagcaggtgg ttactgccta gcagcaactg gaagcgatgg gcggacgaac cctgcttgcg
     3421 ccgcgcttat tacaatcaga gacatcgttg ctgacgccgg ggatcattga aatgacaggc
     3481 gttgctggcg taccagatga agaggtgttc ggaccgttat tgcgcgtctg gcgttatgat
     3541 tctttcgagg aagcgattct aatggcgaat aacactcgct tcggactctc ttgcggtctg
     3601 gtttcccccg agcgggaaaa attcgatcaa ctgttgctgg aggcgcgggt ggggattgtt
     3661 aactggaaca aaccgcttac tggtgctgcc agtaccgcgc cattcggcgg cattggtgca
     3721 tccggtaacc atcgccccag cgcctggtat gccgcagatt actgcgcatg gcctatggcg
     3781 agcctggagt cggactcgtt aactttgccc gcaacgctta accccgggct ggatttttcc
     3841 gatgaggtgg tgcgatgaac gcctgggaag ttaatttcga cgggctggta gggctgacgc
     3901 atcattacgc tggcctgtcg tttggtaatg aagcctctac ccgtcaccgt tttcaggtgt
     3961 ctaacccgcg actggcggcg aaacagggct tactgaaaat gaaaaccctt gccgatgcgg
     4021 gattccccca ggccgtgatc ccgccgcacg agcgtccgtt tattccggtg ctgcgtcagt
     4081 tgggattcag tggtagcgat gagcaggtac tggaaaaagt tgcacgccag gcaccgcact
     4141 ggctttccag cgtcagctcc gcttcgccaa tgtgggtagc caatgcggca acgatcgcgc
     4201 catctgccga tacgctggat ggcaaagtgc atctcaccgt tgccaacctg aacaataaat
     4261 ttcaccgttc gctggaagcg cccgtcactg aatcgctgtt aaaagctatt tttaacgacg
     4321 aagagaaatt tagcgtccat tcggcgttgc cgcaggtagc gttgctcggt gatgaggggg
     4381 cggcaaacca caatcgtctc ggcggtcatt acggtgagcc gggtatgcaa ctttttgtct
     4441 acgggcgaga agaaggcaat gatacccggc cttcccgtta tccggcgcga cagactcgcg
     4501 aagccagcga ggcggtggca aggcttaatc aggtgaatcc ccaacaggtg attttcgccc
     4561 agcaaaaccc ggacgttatc gaccagggtg tttttcataa tgacgtgatt gccgtaagta
     4621 accgccaggt gctgttttgc caccaacagg cgttcgctcg ccagtcacag ttactggcaa
     4681 acctgcgggc gcgggtgaac ggttttatgg cgatagaagt tccggcaact caggtttccg
     4741 tgtcggatgc ggtgtctacc tatctgttta acagccaact gttgagccgc gatgatggtt
     4801 ccatgatgtt ggtgctgcct caggagtgtc gggaacacgc cggagtatgg ggttatctca
     4861 atgaactcct tgccgctgac aacccgatta gcgaactaaa agtctttgat ttacgtgaaa
     4921 gcatggcgaa tggcggcggc ccggcgtgcc tgcggttgcg ggtagtattg acagaagaag
     4981 aacgccgggc ggtgaatccg gcggtgatga tgaacgatac gctgtttaat gcgctcaatg
     5041 actgggtgga tcgttactac cgcgatcgcc ttactgctgc cgatctggcc gacccgcaat
     5101 tgctgcgcga agggcgggaa gcactggatg tattgagcca attactgaat ctcggttcgg
     5161 tttatccgtt ccagcgcgag ggagggggca atggataatt ttcttgctct gaccttaacg
     5221 ggtaaaaaac cggttatcac cgagcgagaa atcaacggcg ttcgctggcg ctggctgggc
     5281 gatggtgtgc tggaactgac gccattaacg ccaccgcaag gcgcgctggt gatttctgcg
     5341 gggatccacg gtaatgagac ggcacctgtg gaaatgctgg acgcgttgct tggcgcgata
     5401 tctcacggcg agatcccgtt acgttggcgg ttgctggtga tcctcgggga tcctcctgcg
     5461 ctgaagcaag ggaaacgtta ttgccatagc gatatgaacc gaatgtttgg cggtcgttgg
     5521 cagctatttg ctgaaagcgg agaaacctgt cgggcgcgcg aactggaaca gtgcctggaa
     5581 gatttttatg accagggcaa agaatctgtg cgttggcacc ttgatctaca taccgcaatt
     5641 cgtggctcct tgcatcctca gttcggtgta ttaccgcaac gcgacattcc ctgggacgag
     5701 aaatttctga cgtggctggg tgcggcgggg ctggaggcgc tggtgttcca tcaggagcct
     5761 ggtggtacgt ttacccattt cagcgccaga cattttggcg cgctggcctg tacgctggaa
     5821 cttggcaaag cgttgccctt tgggcaaaac gatcttcgcc agtttgcagt aactgccagc
     5881 gcaattgctg cgctgctatc tggtgagagt gtcggtatcg tgagaacacc gccgctccgt
     5941 tatcgggtgg tttcgcaaat tactcgccac tcgccgtcct tcgaaatgca tatggcaagt
     6001 gacacgctga attttatgcc gtttgagaaa ggaacattgc tggcgcagga cggagaggaa
     6061 cgttttaccg taacccatga tgtagagtat gtgttattcc ctaatccgtt ggtagcgttg
     6121 ggattacgcg cgggattaat gctcgaaaaa ataagctaat acttacccgc agaaatcatt
     6181 ctgcgggtat taagttgctt tcttataaat taatacagat taatcctggt tatttgc
//
LOCUS       DAZRUF010000228         6188 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZRUF010000228 DAZRUF010000000
VERSION     DAZRUF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.229"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            45..1505
                     /locus_tag="ACHXOL_003582"
     CDS             45..1505
                     /locus_tag="ACHXOL_003582"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR6810468.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1541..1744)
                     /gene="ydfZ"
                     /locus_tag="ACHXOL_003583"
     CDS             complement(1541..1744)
                     /gene="ydfZ"
                     /locus_tag="ACHXOL_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR6810469.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1921..2607)
                     /gene="rspR"
                     /locus_tag="ACHXOL_003584"
                     /gene_synonym="ydfH"
     CDS             complement(1921..2607)
                     /gene="rspR"
                     /locus_tag="ACHXOL_003584"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR6810470.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2696..3442)
                     /gene="ydfG"
                     /locus_tag="ACHXOL_003585"
     CDS             complement(2696..3442)
                     /gene="ydfG"
                     /locus_tag="ACHXOL_003585"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR6810471.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3579..5624
                     /gene="dcp"
                     /locus_tag="ACHXOL_003586"
     CDS             3579..5624
                     /gene="dcp"
                     /locus_tag="ACHXOL_003586"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR6810472.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5669..6187)
                     /locus_tag="ACHXOL_003587"
     CDS             complement(5669..6187)
                     /locus_tag="ACHXOL_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR6810473.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1544 a         1481 c         1593 g         1570 t
ORIGIN      
        1 taacaaaaca tatagcttgc catatttata tttaaggaat tgttatggga aataatttgt
       61 tatcagcaaa agcgacactc cctgtttatg atcgtaataa cctggcccca agaattattc
      121 atttaggctt tggtgcattt caccgtgcgc atcagggcgt gtatgccgat attcttgcta
      181 cagaacattt cagtgactgg ggatattatg aggtcaactt aatcggcggc gaacagcaaa
      241 ttgccgattt acaacagcaa gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt
      301 ggacggctcg cgtcgttggc gtcgttaaga aagccttgca cgtacagatg gatggcttag
      361 aaaccgtgtt ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg
      421 aaaaagggta tttccactct ccggcgaccg gacagttaat gctcgatcac ccgatggtcg
      481 ctgccgacgt gcaaaatccc caccagccga aaacagcaac aggggtgatt gttgaggcgc
      541 tggcccgccg taaagcggca ggacttcccg catttaccgt catgtcatgt gacaacatgc
      601 cagaaaacgg tcatgttatg cgtgacgttg tcacttcata cgcgaaagct gttgatgtaa
      661 aactggcaca atggatcgaa gataacgtga ctttcccatc aacaatggtg gaccgtattg
      721 tgcccgcagt gacagaggat acgctggcga aaatcgaaca acttaccggt gtgggcgatc
      781 ctgcgggcgt tgcctgtgaa cctttccgcc agtgggtaat agaagataac tttgttgccg
      841 gacgtccaga atgggaaaaa gcgggagccg aactggttag tgatgtgctg ccttatgaag
      901 agatgaagtt gcgcatgctc aacggcagtc attcattcct ggcgtatttg ggttatcttg
      961 ctggatatca gcacattaat gactgcatgg aagatgaaca ttatcgttat gcggcgtatg
     1021 gcttgatgtt gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt
     1081 acgctaaccg attaattgaa cgctatagca acccggcgtt acgtcatcga acctggcaga
     1141 ttgcgatgga tggcagccag aaattgccac agcggatgtt ggattctgtt cgctggcatc
     1201 tggcgcatga cagcaagttc gatctgctgg cactgggcgt cgcgggttgg atgcgttatg
     1261 tcggtggtgt tgatgaacag ggaaatccga ttgaaatcag tgacccactg ttacctgtta
     1321 ttcagaaggc tgtacaaagt agtgctgaag ggaaagcgcg cgtccagtca ttgctggcga
     1381 ttaaggcgat ctttggtgat gatttgccag acaatagttt gtttactgca aaagtgacgg
     1441 aagcgtactt gtctttatta gcgcatggcg cgaaagcgac cgtggcgaaa tattccgtga
     1501 agtaaaaaaa gcaatgccgc catcggcggc attcacacgc ttagttcatg ccgagacgaa
     1561 tcaggtccag aggtgccagt ttctcttcac aaccttcaac aactacggtt tttccgcggc
     1621 ggatttgctc agcggtcaga ccttcagtat caatcgacag gatcttgcca gtgtgaccgg
     1681 tgccgctaac cataacacgg ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg
     1741 tcataggtat tttctccttt ctgatttaca gtcgaagccc acgtaacggg cgcaatataa
     1801 atactcctgt cgagtgaaat attttttgtt tttgatcaag ctcacatctt ttttgataca
     1861 tgcatgagcc ataaggcggg ggaaaagact ctcaccccgg atgggatgag aggaaatgaa
     1921 ttactcttcg ctaaaccagt cgctattttc ctggcggatc tggcgcacgg attcgctgat
     1981 ttcctgcaaa tgttgcgtca ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc
     2041 agagaaaata tcaagatgct ggcgtaacag catttctggt ggagaaacgt ggtcgaaact
     2101 catatagcgc acgcgatcaa cggtcgcttt caggttctca atggtatccc acgccagttg
     2161 acagtcggca atctgcgtca ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc
     2221 cagttgcttg cgctcaatgg caatgcgttg ctggtgaaga ttttgttcca gttggtagca
     2281 ctggctttcg gtaatcatgc tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg
     2341 gataaaactg ccattgcgca cctgggccat ggaaattttg ttgacgtagc tgccacgctg
     2401 cggacgaatt tgaatcaggc cgttttccgc cagtttaata aaggcttcac gaaccggctg
     2461 gcgtgacaca ttgaaacgaa cagaaacttc tttttccgac aacggtgtgc ctggtgcaat
     2521 caggcaatgg acaatgtcgc gacgaagaat acgataaatt tgctgattga caggctgtgt
     2581 gggattaagt tgcgtttcga cggtcattcg ttcttgcttg tgagtgagtt aactgcatga
     2641 gtctaccact tttttgtgac aagcaataac ccggcagtta cgccgggtat aaaaattact
     2701 gacggtggac attcagtccg gcatagcttt gggtaaccgg catcatttcc agggtattga
     2761 tattgacgtg agcaggcagc gttgacaccc accagacggc ttcgctgaca tcttctggcg
     2821 tcaatgcaac ggtattttga taggtttttt ccgctttacc gtcatcgcct ttaaagcgga
     2881 cattggaaaa ctcggtgcca cccaccagac ccggttcgat gtcggtgacg cgcaccgccg
     2941 taccatgcag gtccgtacgc agattcaggc taaactgacg aacaaacgct ttcgtcgcac
     3001 cgtaaacgtt accaccggca tacggccagc tacctgccgt tgagccaatg ttaataatat
     3061 gaccatgatt acgttcaacc atacccggta agacggcgcg cgtcatatat accaggcctt
     3121 tgttgttggt atcaatcatc gtttcccagt cttcaacgct ggctttatgc gcaggctcca
     3181 tgcccaacgc caggccggca ttatttacca ggatatcaat attgcaccac tcggcaggaa
     3241 gcgatgccag catctcttca atagcggcgc ggttgcgaac gtccagttgg gcgatataca
     3301 gattatctcc cagttcgtct tttaactcct gcaaccgctc ctggcgacgg ccagtggcga
     3361 taactttatg cccttgttga ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg
     3421 ctccagttac taaaacgatc atctcactgt tcctcaacgc ttttgtgtgt caataacata
     3481 gcacgtgatg cttaagagcg ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa
     3541 gcctacgcta tggaacatct cgcgttcagg agaagaaaat gacaacaatg aatcctttcc
     3601 ttgtgcaaag cacactaccg tatctggctc cccattttga tcaaattgcc aatcatcact
     3661 atcgcccggc attcgatgag ggaatgcagc aaaagcgggc agaaattgct gccatcgcgc
     3721 ttaacccgca aacgcctgat ttcaacaata ctattctggc actggaacaa agcggagaat
     3781 tacttacccg cgttaccagc gtcttttttg cgatgactgc ggcgcatacc aatgatgaat
     3841 tacagcgtct tgatgaacag ttttccgctg aactggcgga actggctaat gatatctatc
     3901 tgaacggtga attattcgcg cgggtagatg ctgtctggca gcgccgtgaa tccctggggc
     3961 ttgatagtga atccatccgc ctggtggagg tgattcatca acgttttgtc cttgccggag
     4021 ccaaacttgc gcaagctgat aaagcaaaat taaaagtact gaatacagaa gctgcgaccc
     4081 tgaccagcca gtttaaccag cgattactgg cagcaaataa atccggcggt ctggttgtga
     4141 acgatatcgc gcagctggca ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg
     4201 ctcgcgagaa aggtctggat aacaaatggc tgattccgct gctgaatacc acccaacaac
     4261 cggcgcttgc cgaaatgcgc gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga
     4321 cgcgagcgga aaaaaatgat ggcaatgata cccgcgctat cattcaacgt ctggtagaga
     4381 ttcgcgcaca gcaggcgaaa ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg
     4441 atcagatggc aaaaacgcca gaagcagcac tcaactttat gcgggaaatt gttccagcgg
     4501 cgcgtcaacg tgcgagcgat gaattagcct ccatacaggc ggttatcgat aagcagcagg
     4561 gcgggtttag cgcgcagccg tgggactggg cattttatgc ggaacaggta cggcgggaga
     4621 aatttgatct tgatgaagcg cagctcaagc catattttga attaaacacg gtgttgaatg
     4681 aaggtgtatt ctggaccgcg aatcagctct tcggtattaa gtttgtcgaa cgttttgata
     4741 ttcctgtcta ccatcctgac gttcgggtgt gggaaatttt tgatcataat ggcgtggggc
     4801 tggcgttatt ttacggtgat ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg
     4861 gcaattttgt tgagcaatca acgcttaatg aaacgcatcc ggtaatttat aacgtctgca
     4921 attatcagaa acccgctgcc ggtgagcctg cgttgttact ctgggatgat gtaataacct
     4981 tattccatga atttggtcat acgctgcacg gcctttttgc ccgccagcgt tatgccacgc
     5041 tttccggcac caacacgccg cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact
     5101 gggcaacgca tccgcaggta ttcgctcgct acgcccggca ttatcagagc ggggcagcaa
     5161 tgcctgacga actgcaacag aaaatgcgta atgccagcct gttcaacaaa gggtatgaga
     5221 tgagcgaact gcttagcgcc gcacttctcg atatgcgctg gcattgcctg gaagaaaacg
     5281 aagcaatgca ggatgtcgat gattttgaat tgcgggcgct ggtggcggaa aatatggatc
     5341 ttcctgctat accgccacgc tatcgcagca gttatttcgc ccatattttt ggtggcggat
     5401 atgctgcagg ttattacgct tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt
     5461 ggtttgttga gcagggcgga ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc
     5521 tttccagagg taacagcgaa gatctggaac gcctgcatcg acaatggcgc ggtaaggctc
     5581 ctcagattat gccgatgctg caacatcgtg gcttgaacat ataaacacca ccaatggctg
     5641 gcctggggat atcgccaggc cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg
     5701 caaacctcac cgctaaagct aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag
     5761 tgtagttctg gcctttaata tgccacgcga accagacggt acccgctggc gtaccatctt
     5821 caccgccctc cggtccgccg tagccggtaa tggcaatact gacatcagca tccgcacgct
     5881 ctatggcacc tgttgccatt tctgctgcca ctttctcact caccgcagaa tatcgttcaa
     5941 gagactgctg gcttacgctg aggattttca tctttgcctg atcggtgaaa gtaacaaagc
     6001 ctgcaccgta aaatttgggt gtatcttcag ctgcacacag ggcgctagcc aacttaccgc
     6061 cggtgcatga ttcggcggta gttaagcgta attgatccgc gataagtctt tgactcaacg
     6121 cggatgtcag gttttcaata gtgtcggtat ctgcgagttg tacaatttta tctttattaa
     6181 tgttcact
//
LOCUS       DAZRUF010000229         6150 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZRUF010000229 DAZRUF010000000
VERSION     DAZRUF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.230"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            16..1326
                     /gene="uidC"
                     /locus_tag="ACHXOL_003588"
     CDS             16..1326
                     /gene="uidC"
                     /locus_tag="ACHXOL_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR6810474.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPIVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(1507..3015)
                     /locus_tag="ACHXOL_003589"
     CDS             complement(1507..3015)
                     /locus_tag="ACHXOL_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR6810475.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(3116..4291)
                     /gene="manA"
                     /locus_tag="ACHXOL_003590"
     CDS             complement(3116..4291)
                     /gene="manA"
                     /locus_tag="ACHXOL_003590"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR6810476.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            4490..6136
                     /gene="fumB"
                     /locus_tag="ACHXOL_003591"
     CDS             4490..6136
                     /gene="fumB"
                     /locus_tag="ACHXOL_003591"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR6810477.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         1541 a         1438 c         1569 g         1602 t
ORIGIN      
        1 atcaaaggtg caactatgag aaaaatagtg gccatggccg ttatttgcct gacggctgcc
       61 tctggcctta tctctgctta tgcggcgcaa ctggctgacg atgaggcggg actacgcatc
      121 agactgaaaa acgaattgcg cagggcggat aagcccagtg ctggcgcggg aagagatatt
      181 tacgcatggg tacagggagg attgctcgat ttcaatagtg gttattattc caatattatt
      241 ggcgttgaag gcggggcata ttatgtttat aaattaggtg ctcgtgctga tatgagtacc
      301 cggtggtatc ttgatggtga taaaagtttt ggctttgccc taggggcagt aaaaataaaa
      361 cccagtgaaa atagcctgct taaatcaggt cgcttcggga cggattatag ttatggtagc
      421 ttaccttatc gtattccgtt aatggctggc agttcgcaac gtacattacc gatagtttct
      481 gaaggagcat taggttattg ggctttaaca ccaaatattg atctgtgggg aatgtggcgt
      541 tcacgagtat ttttatggac tgattcaaca accggtattc gtgatgaagg ggtgtataac
      601 agccagacgg gaaaatacga taaacatcgc gcacgttctt ttttagccgc cagttggcat
      661 gatgatacca gtcgctattc tctgggggca tcggtacaga aagatgtttc caatcagata
      721 caaagtattc tcgagaaaag cataccgctc gacccgaatt atacgttgaa aggggagttg
      781 ctcggctttt acgcgcagct cgaaggttta agtcgtaata ccagccagcc caatgaaacg
      841 gcgttggtta gtggacaatt gacctggaat gcgccgtggg gaagcgtatt tggcagtggt
      901 ggttatttgc gccatgcaat gaatggtgcc gtggtggata ccgacattgg ctatcccttt
      961 tcattaagtc ttgatcgtaa ccgtgaagga atgcagtcct ggcaattggg cgtcaactat
     1021 cgtttaacgc cgcaatttac actgacattt gcacagattg tgactcgcgg ctatgaatcc
     1081 agtaaacgag atgtgcggat tgaaggcgcg ggtatcttag gtggtatgaa ctatcgggtc
     1141 agcgaagggc cgttacaagg gatgaatttc tttcttgctg ccgataaagg gcgggaaaag
     1201 cgcgatggca gtacgctggg cgatcgcctg aattactggg atgtgaaaat gagtattcag
     1261 tatgacttta tgctgaagtc agaaataacg ccggagagaa aaacctccgg cgtttcagat
     1321 tgttgacaaa gtgcgcgttt tttatgccgg atgcggcgta aacgccttat ccagcctaca
     1381 aaaactcata aattcaaagt gttgcaggaa aaggtaggcc tgataagcgt agcgcatcag
     1441 gcaatctctg gtttgttttc agatgaaaac gccgaagtga aaattctccg gcgttttggc
     1501 cgtgaattac tgctgcggaa ttgccggtac agccggaacg ttaagagctg gcatcgcaaa
     1561 cataccaacg aaatcttcca gtggcatttt ctgcccgttt aacgttatct gaccgttagt
     1621 atattgcagg ctggtggtga tggtattgtc ctgcaaggtg gtcagacgga acatctgccc
     1681 cattgctgat gcaccttcaa cttgctgttt cgccagtttt ttcgcttgat cttcctgata
     1741 accttccagc ttcgctacct gagtcataaa ctcagttgcc atatccaccg gaatggtcag
     1801 tttcgcatcc agagatttaa ccgaacgatc tacttcctgc gccagcgttt gcggcgcttc
     1861 tttagtcgtt gccggatctt tcaggaacag cgacagattc agcgcacttt caccctgact
     1921 gtttttccag cttagcggcg cgatagtaat caccggatcg cctttcaaca tcagcggcag
     1981 ggcgctaaag aaggcttccg tcactttctc ctgataaagt tcgggattgt tggcaatttc
     2041 tggctgtgcc agcagcgcct gagtttgcgc gttatattgc tggctaaact gatgccaggc
     2101 ttcaccatca atctggccga cttttaaagt cagcttgccg ctgcccagat cctgattctg
     2161 taccttcagg ctgtttagcg agtaatccag ttggctattg atcgttttac cgtcattgac
     2221 cagatccgat ttaccgctga tctccatgcc ttccagcagt gccagttctt tgccttccac
     2281 tgaaatggtc attttttcca gtgacagttt ttggtttcct acgcgctcac caaaacttgc
     2341 cagcgtgctg gaaccgtcgg ttttcagatt attaaaggtc aactgcactt tctggttgta
     2401 ttcgttaact gcgtctatcc gaccactttg cgcctccccg gaaagggaga tggctttgcc
     2461 gtctctgtca gcatttaact ggaactcgcc gccgctaaag gcgacttttt catccttttg
     2521 ctcgtaattc agtggcttga gcgaaatatc ggaactggaa tcaccgctgt aaccaatgcg
     2581 cgagttaatc tcaaaaggcg tttcaccttt tgccatatca aacagtggtt tgcttacttc
     2641 gttattaacc agcgtggttt gaattgatgc catcgacggg atcaggttca gttttttaag
     2701 ctgggcaagc gggaagggac catgatcaac cgattcgttg aagatgacgc tctgaccgct
     2761 tttaatccac ggattttctt tcccggcaat gggtttcacc aacagttgca actggctgct
     2821 gaatacgccg cgatgatagt tttgataact cacttccagg ttggattcag gagctgtcag
     2881 tttgagttgc gcgttcgcct gcgcgaccat gtcttcgaga tgggtttcaa tcttcttgcc
     2941 tgtataccat gcgccgcctg tccagactac gcctagcgca acaatgacgc ctaccgctac
     3001 cagcgattta ttcataatga ttatccataa aatgaaatca ggcggactgg ccgcctgaag
     3061 gtgttataag cctttaataa gcttagcaag agatgttaat tttttcagta agctcttaca
     3121 gcttgttgta aacacgcgct aaacggccgt ggcctttgac agtcaccggt gattcgttgg
     3181 cggcaataaa cgctgattca cccggtttaa gctgtaactg ctgagaacct ttccacaacg
     3241 ttgcatcgcc ttcgacgcag aacaaaatgg cggcactctg ctggctaatg gtggtttctt
     3301 tatcactaag gtcatgcagc gagaaggcaa aatcatccac tggaatcggg aagtccagtt
     3361 ctgcaccttg tttcaccggc tgggtcaaca actggttagc cggtttggct tcgaatttca
     3421 cattggcaac cagttccgga atatcaatgt acttaggcgt cagacccgca cgcagcacgt
     3481 tatcggagtt cgccatcact tccagcgcca cgccttgcag gtaagcgtgc ggtgtttcag
     3541 cgaacaggaa catcgcttcg ccaggattca atttcaccac attcagtaac agcggggaga
     3601 acaggccact gtcttccggg taaaattcag aaattaaacg aatcgtttgc cacggttcac
     3661 cctgctgact gtcgagcgcc gattttaaaa tcgccagcgc gcgggatttt tcttcaccct
     3721 gcatattcaa caggctggcg aacagttcgc ttaaacgttc ggcattaggc tgttgtaaaa
     3781 agtgagcaat tgccggatgt gcacctgcga ctggctggag tagggagaca atctcggaaa
     3841 actcacgaaa cgcgttcatc gcaaggaaag gcgtcagcgc aaaaaccagc tccggcttgt
     3901 ggttaggatc tttatagtta cgctcggcgg catccatcgg gatacctgcg gcattttctt
     3961 tggcaaaacc gatttcagaa ttgcgtttgt ttggatgaac ctgaatggag agcggctgtg
     4021 ctgcgcataa tactttgaac aggaaaggca gttcgccaaa gcgtttggca acggcctctc
     4081 cgagcagagt cgatttatca ctctcaatca catcacgcag tgaaacgata tctccggcgg
     4141 cattctgcac tcgtgaactg cttttcggat gtgcgcccat ccacagctcg gccatcggct
     4201 ggctggacgg attttccata ccataaagtt cagtcaacgc cgttttgctg ccccaggcat
     4261 agttttgcac tgagttaatg agtttttgca tgatcaatcc ctgttttaat gtggaaatta
     4321 atcccactat taaagcaaga atcctacggg aagtaacctg gagccgcaaa aagtcgtact
     4381 agtctcagtt tttgttaaaa aagtgtgtag gatattgtta ctcgctttta acagggcaac
     4441 ggaacacccg cccagagcat aaccaaacca ggcagtaagt gagagaacaa tgtcaaacaa
     4501 accctttcat tatcaggctc cttttccact caaaaaagat gatactgagt attacctgct
     4561 aaccagcgaa cacgttagcg tatctgaatt tgaagggcag gagattttga aagtcgcacc
     4621 cgaagcgtta actctgttgg cgcgtcaggc gtttcatgat gcgtcattta tgctgcgtcc
     4681 ggctcaccaa caacaggtgg ccgacattct gcgtgacccg gaagccagcg aaaatgataa
     4741 atatgtggcg ctgcaattcc tgcgcaactc cgacatcgcg gcgaaaggtg ttctgccaac
     4801 ctgtcaggat accggcactg cgattattgt tggtaaaaaa gggcagcgtg tatggaccgg
     4861 tggctgtgat gaagcggcgc tggcgcgcgg tgtctataac acttatatcg aagataattt
     4921 gcgctactcg caaaacgcgc cgctggatat gtataaagag gtgaataccg gcaccaatct
     4981 gccagcgcag atcgatcttt atgccgttga tggcgacgag tacaaattcc tctgtatcgc
     5041 caaaggtggt ggttcggcaa acaagacgta tctctatcag gaaaccaaag cgttactaac
     5101 gccggggaaa ctgaaaaatt acctggttga gaagatgcgc acgctgggta cggcggcctg
     5161 tcctccgtat catattgcgt tcgtaattgg tggaacttct gcagaaacta accttaaaac
     5221 agtgaaactg gcttccgcta aatactatga cgaactgcca acggaaggga atgagcacgg
     5281 tcaggcattc cgcgatgtgg aactggaaaa agaattgctg atcgaagcgc aaaatctcgg
     5341 tctgggtgcg cagtttggtg gtaaatactt cgctcacgac atacgcgtga ttcgcctgcc
     5401 acgtcacggc gcatcctgtc cggttggtat gggtgtttcc tgctctgctg accgtaatat
     5461 caaagcgaag atcaaccgtc aggggatctg gatcgaaaaa ctggaacata atccaggtaa
     5521 atatatccct gaagagctgc gtaaagcggg agaaggcgaa gcggtgcgcg ttgaccttaa
     5581 ccgtccgatg aaagagatcc tcgcacagtt gtcgcagtat cccgtttcta cacgcttatc
     5641 gcttaacggc acgattatcg tcggtcgtga tattgctcac gccaaactga aagagcggat
     5701 ggataacggt gaagggctgc cgcagtacat taaagatcat ccgatttact acgcgggtcc
     5761 ggccaaaacg ccggaaggtt atgcctccgg ttctcttggc ccaacgaccg ccggacggat
     5821 ggattcttat gtcgatcaac tgcaagcgca gggcggaagt atgatcatgc tggcgaaagg
     5881 caaccgcagc cagcaggtga cggatgcctg taaaaaacac ggcggcttct accttggcag
     5941 tatcggtggt ccggccgctg tattggcgca gggaagtatt aagagcctgg aatgtgttga
     6001 atatccggaa ctgggaatgg aagccatctg gaaaattgaa gtggaagatt tcccggcgtt
     6061 tatccttgtg gatgataaag gaaatgactt cttccagcag atacaactca cacagtgcac
     6121 tcgctgtgtg aaataaacag agccgccctt
//
LOCUS       DAZRUF010000230         6118 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZRUF010000230 DAZRUF010000000
VERSION     DAZRUF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.231"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            56..832
                     /gene="exuR"
                     /locus_tag="ACHXOL_003592"
     CDS             56..832
                     /gene="exuR"
                     /locus_tag="ACHXOL_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR6810478.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1072..1155
                     /gene="yqiM"
                     /locus_tag="ACHXOL_003593"
     CDS             1072..1155
                     /gene="yqiM"
                     /locus_tag="ACHXOL_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR6810479.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1177..1839
                     /gene="yqjA"
                     /locus_tag="ACHXOL_003594"
     CDS             1177..1839
                     /gene="yqjA"
                     /locus_tag="ACHXOL_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR6810480.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1843..2226
                     /gene="mzrA"
                     /locus_tag="ACHXOL_003595"
     CDS             1843..2226
                     /gene="mzrA"
                     /locus_tag="ACHXOL_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR6810481.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2373..2741
                     /gene="yqjC"
                     /locus_tag="ACHXOL_003596"
     CDS             2373..2741
                     /gene="yqjC"
                     /locus_tag="ACHXOL_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR6810482.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2779..3084
                     /locus_tag="ACHXOL_003597"
     CDS             2779..3084
                     /locus_tag="ACHXOL_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR6810483.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3087..3491
                     /locus_tag="ACHXOL_003598"
     CDS             3087..3491
                     /locus_tag="ACHXOL_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR6810484.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3481..3780
                     /locus_tag="ACHXOL_003599"
     CDS             3481..3780
                     /locus_tag="ACHXOL_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR6810485.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3966..4358
                     /locus_tag="ACHXOL_003600"
     CDS             3966..4358
                     /locus_tag="ACHXOL_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR6810486.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4428..5413
                     /gene="yqjG"
                     /locus_tag="ACHXOL_003601"
                     /pseudo
     CDS             4428..5413
                     /gene="yqjG"
                     /locus_tag="ACHXOL_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            5706..6071
                     /locus_tag="ACHXOL_003602"
     CDS             5706..6071
                     /locus_tag="ACHXOL_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR6810487.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1530 a         1511 c         1541 g         1536 t
ORIGIN      
        1 cccatgaata aagtggtatg ataacttcgt ccgcattcca ctccgggaga cgcatatgga
       61 aatcactgaa ccacgccgtt tgtatcaaca acttgccgct gacctgaaag agcgcattga
      121 acagggcgtc tatctggtgg gtgataaact gcctgccgaa cgctttattg ccgatgaaaa
      181 gaacgtcagc cgaacggtag ttcgtgaagc catcatcatg ctggaagttg aaggctatgt
      241 ggaggtgcgt aaaggttcag gtattcatgt ggtttccaac cagccgcgcc atcagcaggc
      301 ggctgacaat aatatggaat tcgccaatta cggtccgttt gagttgcttc aggctcgcca
      361 gctcatcgaa agtaatattg ccgagttcgc ggcaactcag gtaacgaaac aagacatcat
      421 gaaactgatg gccattcagg aacaggcgcg cggcgaacaa tgcttccgtg attccgagtg
      481 ggatttacag ttccacattc aggtagccct ggcgacgcag aactccgccc tggcggccat
      541 cgttgaaaaa atgtggaccc agcgtagcca taacccgtac tggaaaaaac tacacgaaca
      601 cattgattcc cgtaccgttg ataactggtg tgatgaccac gatcaaattc tcaaggcgtt
      661 aattcgcaaa gatccccatg ctgctaagct ggcaatgtgg cagcatctgg aaaacaccaa
      721 aatcatgtta tttaacgaaa ccagcgacga tttcgagttc aatgccgacc gctatctatt
      781 cgccgaaaac ccggtagtgc atctcgatac ggccaccagt ggcagtaaat gaaactcctg
      841 tccgacaggc gctgcgacgc gcctgttcgc ttcgtcggta agcgaaaagt ataaagtgtc
      901 agcctgtgta aatcctctcg ccaccctccc ctgcattcag caaaatcaga ctccacggac
      961 atgtaatttt gataacgaac aacgttgacc tttgttacaa ttagattcaa tttgaattta
     1021 tgtttttgaa tgctttctta tctcacgatt taacagggaa tagttcaggc tgtgttgatg
     1081 tatcaaaccc gcagaacata ccaaaacagc aataacattg cggtagtgca tcttttaaaa
     1141 ccagcgtggc gttaaccgat tcaccaggaa taatgaatgg aacttttgac ccaattgctg
     1201 caagccctgt gggcgcagga ttttgaaacc ctggccaatc catcgatgat tggcatgttg
     1261 tattttgtct tgtttgtaat tttgttcctt gaaaacggct tgcttccagc ggccttttta
     1321 ccgggcgaca gtttactggt attggtcggc gtgttgattg cgaaaggcgc gatgggttac
     1381 ccgcaaacga ttctgctgct gaccgttgcc gccagcctcg gctgctgggt cagctatatt
     1441 caggggcgat ggctgggcaa tacccgcacc gtacaaaact ggctatctca tttacccgcg
     1501 cattatcatc aacgcgcaca ccatcttttt cataaacacg gtttatcggc gctgttaatt
     1561 ggtcgcttta ttgcgtttgt cagaacactg ctgccgacga ttgccgggtt atcagggctg
     1621 aataacgcgc gctttcagtt tttcaactgg atgagcggtc tgctgtgggt attgatcctg
     1681 acaactctgg gttacatgct cggcaaaacg ccgatatttt taaagtacga ggaccagctg
     1741 atgtcatgcc tgatgctgct cccggtggtg ctgctggtgt ttggcctggc aggttctctg
     1801 gtcgtgttat ggaaaaagaa atatggaaat cgggggtaag ggatgcaaat acctcgcatg
     1861 tcgcttcgcc agctagcctg gtccggcact gttttacttc tggtcggcac gctgttgctg
     1921 gcctggtccg cggttcgcca gcaagagtct acgctggcga ttcgtgccgt tcatcaaggc
     1981 acaacgatgc cagacggttt ttcgatctgg catcaccttg acgctcatgg cattcctttc
     2041 aaaagtatca cccccaaaaa cgacactctg ttaattacat ttgactccag cgaccagagc
     2101 gccgccgcaa aagcggtcct cgacagaaca ttgccccatg gctacatcat tgcgcagcag
     2161 gacaataaca gtcaggctat gcagtggctg acccggttac gggataactc tcatcgcttc
     2221 ggataacttc ctgttatccg aaacatatca ctcactttgg tgatttcacc gtaactgtct
     2281 atgattaatg aggcggtggt taacactacc gcttcgttgt ctggacaatc gttcctttgt
     2341 aataggtccg aatcacaatg gaaggttcaa gaatgaaata ccgcatcgct ttagctgttt
     2401 ctctctttgc tcttagtgcc ggtagttatg ccactaccct gtgtcaggaa aaggagcaaa
     2461 atatccttaa ggagatcagc tatgccgaaa aacaccaaaa ccagaatcgt attgacggtc
     2521 tgaataaagc cctgagtgaa gtccgggcca actgttcaga tagccagctg cgtgccgatc
     2581 atcacaagaa aatcgcaaag cagaaagatg aggtggcgga acgccagcaa gatttagccg
     2641 aggcgaagca aaaaggcgat gccgataaga ttgccaaacg cgaacggaaa ctggcagaag
     2701 cgcaggaaga gctgaaaaag ctggaagcgc gcgactacta actcacaata gtcactactt
     2761 actcacctgg agaaaactat gtcgaaagaa cacactacgg aacatctgcg tgctgagttg
     2821 aaatcccttt ccgatacgct ggaagaggtg cttagctcat ctggcgagaa gtcgaaagaa
     2881 gagttgagta agattcgtag caaagcggag caggcactga aacagagccg ttatcgcctg
     2941 ggtgaaaccg gtgatgccat cgccaaacaa acccgtgtcg cggcggcgcg tgctgatgag
     3001 tatgtgcgtg aaaatccgtg gaccggcgtg ggaattggcg ctgcaatcgg tgtagtgctc
     3061 ggcgttctgc tgtcgcgtcg ttaattatgg cggacactca tcacgcgcaa gggcccggta
     3121 aaagcgttct gggcatcggg cagcgaattg tttccatcat ggttgaaatg gtggaaacac
     3181 gtctgcggct ggcggtggtg gagctggaag aggaaaaagc taatctcttt caacttttac
     3241 tgatgctggg cctgacgatg cttttcgctg catttggtct tatgagcctg atggtgctaa
     3301 ttatttgggc ggttgatccg caatatcgcc tgaatgcgat gattgccacc accgtggtgt
     3361 tgctgctact ggcactgatt ggcggtatct ggacgttacg taaatcgcgt aaatccacct
     3421 tgctgcgcca tacacgccat gagctggcaa acgatcggca actgctcgag gaggagtccc
     3481 gtgagcagta aagtcgaacg tgaacgacgt aaggcgcaac tgcttagcca gatccagcaa
     3541 caacggctgg atctttccgc cagtcgtcgt gaatggctgg aggcaacagg cgcttacgat
     3601 cgtcgctgga atatgctgct aagtctgcgc tcctgggcgc tggttggcag tagcgtgatg
     3661 gcgatctgga cgattcgcca tcctaatatg ctggtccgct gggccagacg cggttttggc
     3721 gtatggagcg cctggcgtct ggttaaaacg accctcaagc agcaacagct tcgcggttaa
     3781 cttccctctg gccggagcaa ttccggcctt atccctcaaa ttttttgaag atttttgaca
     3841 gttttccttg ctaacaatca tcattcacca cgtttatgat tctctccatc gacagcaacg
     3901 acgctaatac cgcgcccttg cacaaaaaaa caatcagcag cctgagtggc gcagtggaga
     3961 gtatgatgaa aaaattagaa gatgttggtg tactggtagc gcgcatttta atgccgattc
     4021 tgtttattac cgcaggctgg ggaaaaatca ccgcttactc tggtacccaa caatatatgg
     4081 aagcaatggg tgtcccgggt tttatgctgc cgctggtgat tctgcttgag ttcggtggtg
     4141 gtctggcaat cctgttcggt ttcctgactc gcaccacagc cctgtttact gcgggcttta
     4201 cgctgctgac ggcattttta tttcacagca actttgctga aggcgtcaac tcgctgatgt
     4261 tcatgaaaaa cctgacaatt tctggcggat tcctgctgct ggcaattacc ggtccgggcg
     4321 cgtatagcat cgaccgcctg ctgaataaaa agtggtaagc acgcgctata cttaacgata
     4381 aaaaacgagg aggaagctcc tcgtttttgc tattggagga gagaaaaatg ggtcaactga
     4441 ttgacggcgt ctggcatgac acctggtacg ataccaaatc taccggcggt aaatttcaac
     4501 gttcagcttc cgcatttcgt aactggctca ctgccgatgg cgcacctggc cccactggca
     4561 caggcggttt tatcgcagag aaagatcgtt atcatctcta tatttcactc gcctgcccgt
     4621 gggcgcaccg cacgctgatc atgcgcaaac tcaaaggact ggaaccgttt atttccgttt
     4681 ccgtagtgaa cccgctgatg ctggaaaacg gctggacctt tgatgacagt tttccgggag
     4741 caaccggaga tacgctctat caacatgaat ttctgtatca gctttatctc cacgccgatc
     4801 cacactacag cggacgagtt actgttcccg tactgtggga caaaaagaac cacaccatcg
     4861 tcagcaacga atcaacagaa atcatccgca tgttcaatac cgcgtttgat gcgctgggcg
     4921 cgaaagcggg tgattactac ccaccagccc tgcaaacgaa aattgacgaa cttaacggct
     4981 ggatttatga caccgttaac aacggcgtgt ataaagctgg ttttgccacc agccagcaag
     5041 cctacgacga ggcggtggcg aaagtgtttg aatcgctggc gcgactggag cagattttag
     5101 gccagcatcg ttacctgacc ggcaaccagc taaccgaagc cgatattcgc ctgtggacta
     5161 cgctggtgcg ttttgatcca gtgtatgtga cccacttcaa gtgtgataag caccgcatca
     5221 gcgattacct gaatctgtat ggcttcctgc gcgatatcta ccagatgccg gggatcgccg
     5281 aaactgtcaa tttcgatcac atccgtaatc attacttccg cagccataag accatcaacc
     5341 ctacggggat tatttcaatt ggtccgtggc aggatctcga tgaaccgcag gacgagatgt
     5401 tcgcttcggt taaaaaaagg caccttacga agtgcctctg ataattagct aagtgcatgt
     5461 tgttcatatc ggatgcggcg tgaacgcctt atcaaaccta caaaacattt atattcaata
     5521 tgttgcaatg accgcgcagg ccagataagc gtagcgcatc tggcgatttt gcgcttgtca
     5581 tcagtcttag cgcgccttca cagcacttta ttaattcctt gaacgaatat ttactgccat
     5641 ttcattcacg tctattctta atttgctgct ttaaaaacaa gtgattgagc aaaattgagg
     5701 caaaaatgga ctggtatctg aaagtaataa aaaattatgt cggtttccgg ggtcgtgcgc
     5761 ggcgcaaaga gtactggatg tttattctgg tcaacatcat ctttacgttc gtgctggggc
     5821 tgctggataa aatgttaggc tggcaacgcg ctggtggcga aggcatcctg acgacaatct
     5881 acggtattct ggtgttttta ccgtggtggg cagttcagtt ccgccgcctg cacgacaccg
     5941 accgctcggc gtggtgggca ctgctattct taatcccgtt tatcggctgg cttattatca
     6001 tcgtctttaa ctgccaggcg ggtacgccag gcgaaaaccg ctttggacct gatccaaaac
     6061 tggaacctta aaacaaatct gctcgccgga tgtctgactc atccggcttt tatcatca
//
LOCUS       DAZRUF010000231         6088 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZRUF010000231 DAZRUF010000000
VERSION     DAZRUF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.232"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(187..1857)
                     /gene="betA"
                     /locus_tag="ACHXOL_003603"
     CDS             complement(187..1857)
                     /gene="betA"
                     /locus_tag="ACHXOL_003603"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFR6810488.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPTLDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1871..3343)
                     /gene="betB"
                     /locus_tag="ACHXOL_003604"
     CDS             complement(1871..3343)
                     /gene="betB"
                     /locus_tag="ACHXOL_003604"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFR6810489.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3357..3944)
                     /gene="betI"
                     /locus_tag="ACHXOL_003605"
     CDS             complement(3357..3944)
                     /gene="betI"
                     /locus_tag="ACHXOL_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFR6810490.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4083..6035
                     /gene="betT"
                     /locus_tag="ACHXOL_003606"
                     /pseudo
     CDS             4083..6035
                     /gene="betT"
                     /locus_tag="ACHXOL_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
BASE COUNT         1228 a         1694 c         1702 g         1464 t
ORIGIN      
        1 gcagataagg ttgtattaat agtctgtatc aggaatgttc ggggtaaata tcagcaaaaa
       61 accagcatca tgaatactgg atatgaagca tgagagttac ctcagtgttt atataaggat
      121 tcggtccccc tctctggaac ggtaactctc aatctgatcg gttcctgcgt tagttcacat
      181 cacgactcat tttttcgctc tcaccggcat cccatttgcc acaaaatatc ccgccgtgct
      241 cctcggcagc gcttcctgtc cacgaatcat atccgctatt ttctcgccaa tcataattgt
      301 cgtggcgttc aaattcccgg tgataatctg cggcataatt gacgcatcca ccacgcgcag
      361 gccttccagc ccatgcacgc ggccttcgcc gtcaaccacg gacatctcgt cgtaacccat
      421 tttgcaggta ccgcacggat ggaaggcggt ttcggcgtga ttacgcacga actcatcgag
      481 ctgctcatcc gtctgacatt ccgtgccggg gctgatttcg cggccacgat actgatccag
      541 cgtcggttga tgcatgatct cccgggtgat gcgaattgcg tcgcggaact cctgccagtc
      601 ctgctcgtgt gacatgtagt taaacagaat tgccggatgc tggtgcgggt cgcgggattt
      661 aatccgcaca tgcccacggc ttggcgagcg cattgagccg acgtggcact ggaaaccgtg
      721 ctccttcacc gcattcgagc cgttatagtt aatcgctact ggcaggaaat ggtactgaat
      781 attcggccac gcaaattcct ctcggctgcg aataaatccg cctgcttcaa agtggttgct
      841 ggcaccaacg ccagtgccgc caaacagcca ctccgcaccg attttcggct ggttccacca
      901 ctgcagggca gggtagaggg aaaccggttc tttgcactca tattgcagat acatctccag
      961 atgatcctga agattttcgc cgacgccggg taattcatgc accagcggaa tatcaaactc
     1021 cgccagcagt tcagcgttgc cgacgccgga gcgttgcagg atctgcggtg aggcaatcgc
     1081 gcctgcacat aacagcactt ctttgttggc cgttgcgcgg gttgggatgg tgctgtcgcc
     1141 ttccagccat tcgacgccca ccgcgcgttt gccgtcaaaa atgatgtgat cggtcatagc
     1201 gtgagtacga atggtcaggt tagggcgcga tttggcctga tcgagataac cgcgcgcggt
     1261 actggcgcga cggccctgcg gcgtgacggt gcgatccatc ggaccaaaac cttcctgctg
     1321 ataaccgttg agatcgtccg tgcgcgggta gcccgcctgc acgcccgctt caatcatcgc
     1381 ttcaaacagc ggattgacgc cgggtttgga ggtagtgacg ctcaccgggc catcgccacc
     1441 gtggtagtcg ttctcgccca catcgcgagt ctcggccttg cggtagtagg gcaggcagtc
     1501 gagataactc cagttctcca gaccgggttc ttgcgcccag ttatcgagat ccagcgcatt
     1561 gccacggatg tagcacatgc cgttgatcag cgacgatcca cccaggcctt taccgcgtcc
     1621 gcactccatg cggcggttat tcataaacgg ttcaggctct gtttcatagg cccagttgta
     1681 gcgtttgccc tgtagtggga acgccagcgc tgcgggcatc tgggtgcgga agtcaaagcg
     1741 atagtccggg ccgcccgctt caagcagcag cacggaggta ttcggatctt cagtcagacg
     1801 ggtagcgaga acgttgccgg ctgagccggc accaataatg atgtagtcaa attgcaaata
     1861 aacctcctgg ttagaatatg gactggaatt tagccatctc aacctggatg gacttcacct
     1921 gggtgtaact ctggagcgtc atcacgccgt tctcgcgacc aatgccggag tgtttgtagc
     1981 cgccaacggg catctctgcc ggggattcgc cccaggtgtt gatccagcaa atacccgctt
     2041 ccagctgatg aatgacgcga tgcgcgcggt tcaggtccgc cgtcacgatg cccgccgcca
     2101 ggccgtagtc ggtatcattg gcgcggcgaa tcacttcgtc ctcagattca taagtgagaa
     2161 gcgacatcac cggaccgaag atctcttcgc gcacgatggt catttcgtcg cggcaatcgg
     2221 tgaacacggt cggtgcaacc catgcgccgt tatcgaagcc atcgcctttc agtacatcgc
     2281 cgccgcacag tacgcgcgcg ccttcctctt tgcctttggc gatatagcgc agcacgttat
     2341 cgcgatgcgg gaagctgacc agcgggccga agttagtttg cggatcgaaa acgtcgcccg
     2401 cgcgaatgcg ctcaacgcgc gccagaattt tctgctcaaa tgcggctttg catttcgccg
     2461 gaacgaagac gcgggtgcca ttggtacaca cctgaccgga gctgaagaag tttgccatca
     2521 tggcgatatc ggcggcgaga tcgagatccg catcatcgaa aacgatcagc ggtgatttac
     2581 cgcccagttc catggtcact tctttcaggg aagaggccgc cgagttagcc atcacttttt
     2641 tgccgctggc gacaccgccg gtaaatgaca ctttggcaat gcccggatgc tcggtcagat
     2701 attgcccggt ctccgcgccc acccccggca acacgttaaa tacgccgtcc ggcaggcccg
     2761 cttcgctgta aatttcagcc agctttaacg cggtaagcgg ggtgacttcg ctcggtttga
     2821 aaatcattgc gttgcctgcc gccagcgccg gggcggattt ccacagggca atctgaatcg
     2881 ggtagttcca tgcgccaatc cccgccacca cacccagcgg ttcgcggcgg gtataaacaa
     2941 atgacgtctc acgcaacggg atctggctgc cttccagcgc cgggatcagc ccggcgtagt
     3001 actccagcac gtccgcaccg gtaacgatat cgacggttga ggtttccgaa tatgcttttc
     3061 cggtatcgag ggtttccagt tttgcgagtt cgtcattgcg ttcacgcaga atatcaacgg
     3121 cccgacgcag aatacgcgag cgctccatag cggtcatcgc cgcccagatt ttttgcccct
     3181 gttgggcact cttcacggcg cgatcgacat cctcacgccc ggcggcctgc acggtcgcca
     3241 gcacgttacc gttggccggg ttaatggtct cgaaggtgcg accgctggtg gcggaggtat
     3301 aaccaccatg tatataaagc tgctgttctg ccattcggga catcacgtct cctcggttaa
     3361 tcggtgggta aatgctgagt aataaagtga cgggtcagcg agtgtgcgag aggtttatcc
     3421 agcggtttgc cgcttagtgc cgcgcgcagc cataatccgt caatcagcgc ggcaaggccg
     3481 tagcccgctt cctgtgcctg ttcgcgcggc aattcgcggc gaaactcgct caccagattc
     3541 gacagcaagc ggcggctgct gacctgctgt aaacgataga gcatcggctg atgcatactg
     3601 ctggcccaga acgccagcca ggctttcatc gccgcactgc tcacctgagt ttcatcgaag
     3661 tttccggcaa caatcgcctg taatcgctgc tctgcactgc cctgcggaag tgcatgtaat
     3721 cgattcaaaa ccgcgtcacg cagctgactg gtgatatcgc gcatggttgc ttccagcaga
     3781 ccatttttgt ccctgaaata gtggctgatg atccccgtag aaacgcctgc acggcgggcg
     3841 atctgcgcga tcgttgcatc gtgcatgccc acttcattta ttgcttccag tgtggcgtcg
     3901 atcagttgtc tgcgccggat cgactgcatc cccaatttgg gcattttcgc cactccattc
     3961 atcagcgttg tttatctatt aaagcggtta ttgattggac gttcaatata aaatgtgtcg
     4021 taattgttac gaatttgatt ttaaatggta acaataacag tggggatact ggatgacaga
     4081 ccttttccct gacaacgatc ctgtttcgcg acttctcggc cctgtggatt ggccgcacgc
     4141 tggactgggt ttctaaaacc ttcggttggt actatctact ggcggcaacg ctctatattg
     4201 tctttgtggt ctgtatcgct tgttcgcgtt ttggttcggt gaagctcggg ccagaacaat
     4261 ccaaaccgga attcagcctg ctgagttggg cggcgatgct gtttgctggc gggatcggta
     4321 tcgacctgat gttcttctcc gtagccgaac cggtaacgca gtatatgcag ccgccggaag
     4381 gcgcgggaca gacgattgag gccgcgcgtc aggcgatggt ctggacgctg tttcactacg
     4441 gcttaaccgg ctggtcgatg tatgcgctga tgggcatggc gctcggatac tttagctatc
     4501 gttataattt gccgctcacc atccgctcgg cgctgtaccc gatcttcggt aaacggatta
     4561 acgggccgat aggtcactca gtggatattg cagcggtgat cggcactatc ttcggtattg
     4621 ccactacgct cggtatcggt gtggtgcagc ttaactatgg cttgagcgta ctgtttgata
     4681 ttcccgattc gatggcggca aaagcggcac tgatcgcctt gtcggtgata atcgccacga
     4741 tctctgtcac ctccggtgtc gataagggca ttcgtgtgtt atcggaactt aacgttgcgc
     4801 tggcgctggg attgatcctg ttcgtattgt ttatgggcga cacctcgttc ctgcttaatg
     4861 cgctggtgct gaacgttggc gactatgtga atcgctttat gggcatgacg ctcaacagtt
     4921 ttgccttcga ccgtccggtt gagtggatga ataactggac gctcttcttc tgggcatggt
     4981 gggtggcatg gtcgccgttt gtcggcttgt tcctggcgcg tatctcgcgt gggcgtacca
     5041 ttcgccagtt cgtgctgggc acgttgatta ttccgtttac cttcacgctg ttatggctct
     5101 cggtattcgg caatagcgcg ctgtatgaaa tcatccacgg cggcgcggca tttgccgagg
     5161 aagcgatggt ccatccggag cgcggcttct acagcctgct ggcgcagtat ccggcgttta
     5221 cctttagcgc ctccgtcgcc accattactg gcctgctgtt ttatgtgacc tcggcggact
     5281 ccggtgcgct ggtgctgggg aatttcacct cgcagcttaa agatatcaac agcgacgccc
     5341 ccggctggct gcgcgtcttc tggtcggtgg cgattggtct gctgacgctc ggcatgctga
     5401 tgaccaacgg gatatccgcg ctgcaaaaca ccacggtaat catggggctg ccgttcagct
     5461 ttgtgatttt cttcgtgatg gcggggttgt ataaatctct gaaggtagaa gattaccgcc
     5521 gtgaaagtgt caaccgcgat accgcgccgc gaccgctggg gcttcaggat cgcctgagct
     5581 ggaaaaaacg tctatcgcgc ctgatgaatt atccgggcac gcgttacact aaacagatga
     5641 tggagacggt ctgttacccg gcaatggaag aagtggcgca ggagctgcgg ttgcgcggcg
     5701 cgtacgtgga gctaaaaagc ctgccgccgg aagagggaca acagttgggg catctggatt
     5761 tgttggtgca tatgggcgaa gagcaaaact ttgtctatca gatttggccg cagcaatatt
     5821 cggtgccggg ctttacctac cgcgcacgta tcggtaaatc gacctactac cgactggaaa
     5881 ccttcctgtt agaaggcagc cagggcaacg acctgatgga ctacagcaaa gagcaggtga
     5941 tcaccgatat tcttgaccag tacgagcggc accttaactt tattcatctc catcgtgaag
     6001 cgccgggcca tagcgtgatg ttcccggacg cgtgattgtt tttgcataaa accgcgccat
     6061 gtctgcatat ggcgcgggaa tcattgac
//
LOCUS       DAZRUF010000232         6081 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZRUF010000232 DAZRUF010000000
VERSION     DAZRUF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.233"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..852)
                     /gene="melB"
                     /locus_tag="ACHXOL_003607"
     CDS             complement(<1..852)
                     /gene="melB"
                     /locus_tag="ACHXOL_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR6810491.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLV"
     gene            complement(955..2310)
                     /gene="melA"
                     /locus_tag="ACHXOL_003608"
     CDS             complement(955..2310)
                     /gene="melA"
                     /locus_tag="ACHXOL_003608"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR6810492.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2593..3501
                     /gene="melR"
                     /locus_tag="ACHXOL_003609"
     CDS             2593..3501
                     /gene="melR"
                     /locus_tag="ACHXOL_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR6810493.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3700..5967
                     /gene="adiA"
                     /locus_tag="ACHXOL_003610"
     CDS             3700..5967
                     /gene="adiA"
                     /locus_tag="ACHXOL_003610"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR6810494.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         1617 a         1608 c         1446 g         1410 t
ORIGIN      
        1 tactaacgtc accaggttag cagctcccgc atacgacaga taatagggga acaaatccgc
       61 atcaccgata acatatgaga aataatagat agcaaatccg gtaataatgt tgctggctac
      121 attataagca agagccatac ccaagaggca tgaaagctga tcgtttttat aaattagcgc
      181 aacgatggct ttaagtgtca gatggcttcc ttcagcagac ggttgattgt ctgacgaaaa
      241 gacttcatgc acattgcgca gagtgatgat ggttgaaaca ataaaaaagg cgatcagtac
      301 cagtgtgaac atctgaaagc caaatccccg atcgccaccg ccgacataat taacaaatgg
      361 tagcgtcaca cctgccgtaa caaagcccgc cagactggca aaaaaacgcg gataaggaac
      421 cagttgttca cgctcacgtt tatcgagcgt gatggttgga accagcgacc agaagggaat
      481 atccataatg gtgtaagtca tgccccagag gatgtaggtc acgcaaacaa agacaatctg
      541 agtagtacct tcaaacagat gcgcactaaa gaggagaaat aagattacag agtttgccaa
      601 cgtaccgatc aggatccagg gtttgaactt accccatcgc gatcgcgtag catttacaat
      661 ccatcccata atcggatcgt taatagcatc ccagatcctc gccaccagaa acaaagtacc
      721 gaccaaaccc acagacagcc caacgacatc ggtgtagtaa tacatgaggt acatatacac
      781 aatgccgatc gcaaaatcct tcccgaacgc tccaaatcca taactgagtt ttgtagtcat
      841 tgaaatgctc atagggtatc gggtcgctgt taccaacgcc cgccttctgt tattggtatc
      901 gtcagaggtg tgcgccggaa taaatccccc ggcgcagtag cgtttagtcg cgttttaacg
      961 gtgcaaccag cctggcagcc agtcgccgtg ggcggcaatc aggtcgtcaa caagagcata
     1021 tatttcgtca atgcccagca cggcggcagt atgcgggtcc atcatcgcgg cgtggtaaac
     1081 acggtcgcga ttttccgtaa gaatagcttc ggtcagcagc gtctgtacat tgatgttggt
     1141 ttgcatcagg gcggccagat gcgaaggtag cgtaccgact ttagtcggct gaatgccatt
     1201 agcatcaacc agacaggcta cttccacgca acatccttgt ggcaggttat caatcaaacc
     1261 atcgttacgg acgttgccgt aaatcacact cggctcgcca gtccagatag cgttcatgat
     1321 tgtgctggca tattctcgtg acggtttaat atcaatccgg gaggcgtttt tatactcctc
     1381 cagctcttta tgccagttcg ccagctgctc gacgcagcgt ttcgggtact catccagcgg
     1441 tactttataa cgctcaatca aatcctcacg acctggctta ataaaccacg gcgtgtattc
     1501 agcaaaatgt tctgacgatt ccgtaacgaa gtagcccagc tttttgaaca tttcgtagcg
     1561 cacaatattc tggcagcgag tattaccatg aatatttggc ttcggtgcct gccctgcttc
     1621 ataagccgcc agcagttccg ggtagagatt cacgtaactg ccgtcggcgg ttttacgctc
     1681 cagttccagg taaaacgcca tatggttgat accagcgcaa cggtaacgca gcgtggacgg
     1741 atcgatattg aggtcacgcg ccagctcttc cgccgttccc tgcaccgaat ggcacagccc
     1801 tacctgtttg atatgcggat agcgggcata catcgcccag gtattcatcg ccattgggtt
     1861 aacgtagttg agcatggtgg catcggggca gacttccgtc atgtcctcgc aaatttgcca
     1921 cagatgcgga atggtacgta gcgcgcgcat aataccgccc ggccccaacg tatcggcaat
     1981 ggtttgttcc agaccatgcc gcttacagac ctcgaaatca gtcaccgtgc aaggttcata
     2041 accgccaatc tgaaatgcca ccacgacaaa atcggcatcc tgtaaggctt ctttctgttg
     2101 ggtgtggcag gtgattttgc cgctggcccc tgctgaatcc atcagcttac gcaccacaat
     2161 atgcgactct tccaggcggg tgggatcaat gtccatcagg gcaatatgcg ccgttttcag
     2221 cgcctcgcga tggaacacat caccaagaat atttttaacg aaaatcgtcg aaccagcgcc
     2281 gataaatgta attttgggtg cagtcatcat gcagatctcc tggcttgctt gaataacttc
     2341 atcatggcag gcgaatctgc agaaaacctc cggcttccag gttgagcatt cccataaact
     2401 cagatcctgc gtgaagcagc agtaaatctg agtttatggt aataagttac ctgaaaagca
     2461 gagttttcgg cctcatgatg ctcggacgct cccctcgcct ctctgggaag agggtgaaaa
     2521 ccgtgctccc actcgcagtc atcctccctc actcctgcca taattctgat attccaggaa
     2581 agagagccat ccatgaatac agatacgttt atgtgcagca gcgacgaaaa acagacccgc
     2641 agcccgctgt cgctgtattc ggaataccag cggatggaga ttgagtttcg cgcaccgcat
     2701 atcatgccca ccagccactg gcatggtcaa gtcgaagtga atgtgccttt cgatggcgat
     2761 gtggaatacc tgatcaacaa tgaaaaagtg aatatcaatc agggtaatat cacgctgttc
     2821 tgggcctgta caccgcacca actaacagat accggaacct gtcagagcat ggcgattttt
     2881 aatctgccga tgcatctgtt tctctcctgg ccgctggata aagacctgat taaccacgtc
     2941 actcacggca tggtgatcaa atcactggcc acacagcaac ttagcccgtt tgaagtgcgc
     3001 cgctggcagc aggaattaaa cagtccgaac gagcaaattc gccagctcgc cattgatgaa
     3061 attggcctga tgctcaagcg atttagcctc tctggctggg aaccgattct ggtcaataaa
     3121 acctcgcgca cacacaaaaa cagcgtctcg cgccatgcgc aattttatgt tagccagatg
     3181 ctgggcttta ttgccgaaaa ctatgatcag gcgctgacca tcaacgatgt ggctgagcac
     3241 gtcaaactta acgccaacta tgcaatgggg atattccagc gggtcatgca attgacgatg
     3301 aaacagtaca ttaccgcgat gcgcatcaac cacgttcgcg cgttactgag cgataccgat
     3361 aaaagtattc tcgatattgc cctgacggca ggctttcgtt caagtagtcg tttttacagc
     3421 acgttcggca aatatgtcgg catgtcgccg caacaatacc gcaaacttag ccaacagcgc
     3481 cgccagacgt ttcccggcta aagacgatat cagtatcagc caaaaaaata gtttagccgt
     3541 cgataataag cagtgaaaat aatttaaaat aatcacataa cgcattgttt ataaaaataa
     3601 atatttcaaa aatgtttgtt tttcacgcgc tttacagccc gaaaaggccg gaagatactt
     3661 gcccgcaacg aagattcctt cataaccggg taagcaatga tgaaagtatt aattgttgaa
     3721 agcgagtttc tccatcaaga cacctgggtc ggtaacgccg ttgagcgtct ggcagatgct
     3781 ttaagccagc aaaatgttac cgtgattaaa tccacctcct ttgatgatgg ttttgccatt
     3841 ctctcttcaa acgaagccat tgactgcctg atgttcagct atcaaatgga acatccggac
     3901 gaacatcaaa acgtcagaca attgatcggt aagcttcatg agcgccaaca aaacgtgccg
     3961 gtcttcctgt tgggcgatcg ggaaaaagcc ctcgccgcaa tggatcgcga cctgctggag
     4021 cttgtcgatg aattcgcctg gattctggaa gataccgccg actttatcgc cggacgcgct
     4081 gttgccgcga tgacccgcta ccgccagcag ctgttgccgc cactgttcag cgcgctgatg
     4141 aaatatagcg acatccatga atattcctgg gcagcgccag gccaccaggg cggcgttggt
     4201 tttaccaaaa cacccgccgg acgtttctac catgactact atggtgaaaa tctgttccgc
     4261 accgatatgg gcatcgaacg aacttccctc ggttctttgc ttgaccatac tggcgcattt
     4321 ggcgaaagcg aaaaatatgc cgcacgcgta tttggtgccg atcgctcctg gtcggtagtc
     4381 gtcggtactt ccggctctaa ccgcaccatc atgcaggctt gcatgaccga taacgatgtc
     4441 gtggtcgttg accgtaactg ccataaatcc atcgaacaag gcttgatgct gacaggcgcg
     4501 aaaccggtct acatggtgcc aagccgcaac cgctacggca ttatcgggcc aatctatccg
     4561 caggaaatgc aacctgaaac cttgcagaag aaaatcagtg aaagcccgct gactaaagac
     4621 aaagccgggc aaaaaccgtc ttactgcgtg gtgaccaact gcacctatga cggcgtgtgt
     4681 tataacgcta aagaagcgca ggatctgctg gaaaaaacct ccgatcgtct gcactttgac
     4741 gaagcctggt acggctatgc acgtttcaac ccgatctatg ccgatcacta tgccatgcgc
     4801 ggcgaacctg gcgatcacaa cggtcctacc gttttcgcca cccactccac ccacaaactg
     4861 ctgaatgcgc tttcacaggc ttcttatatt catgtacgtg aaggtcgtgg ggcgattaac
     4921 ttctcccgct tcaaccaggc ctacatgatg catgccacca cctccccgct gtatgccatc
     4981 tgcgcatcca acgacgtggc ggtgtcgatg atggacggca acagcggcct gtcactgacg
     5041 caggaagtga tcgacgaagc ggttgatttc cgtcaggcga tggcgcggct gtataaagag
     5101 ttcaccgctg acggtagctg gttcttcaaa ccgtggaaca aagaagtcgt caccgaccca
     5161 caaaccggca aaacctatga ctttgctgac gcaccaacca aactgctgac caccgttcag
     5221 gactgctggg taatgcatcc gggcgaaagc tggcacggct tcaaagatat tccggataac
     5281 tggagtatgc tcgacccgat taaagtcagc atccttgctc cgggaatggg tgaagatggt
     5341 gaactggaag aaaccggtgt tccggcggcg ctggtcactg cctggcttgg tcgccacggc
     5401 attgtgccta cccgcaccac tgacttccaa attatgttcc tgttctctat gggcgtaacc
     5461 cgtgggaaat ggggaactct ggttaacacc ctttgctcct tcaaacgcca ctatgacgcc
     5521 aacacaccgc tggcgcaggt gatgccggaa cttgttgaac aatatcctga cacttacgcg
     5581 aacatgggga ttcacgatct gggtgacacc atgtttgcct ggctgaaaga aaacaaccct
     5641 ggcgcacggt tgaacgaagc ctattccggc ctgtcggtgg cggaaatcac cccgcgtgaa
     5701 gcgtacaacg cgattgtcga caacaatgtc gaactggtat ccattgaaaa tctgccagga
     5761 cgcatcgcgg caaactcagt tatcccatat ccgccaggaa tcccgatgct gctgtctggt
     5821 gaaaacttcg gcgataaaaa cagtccgcaa gtaagttatt tacgctcgct gcaatcctgg
     5881 gaccaccatt tccctggatt tgaacacgaa actgaaggga ctgaaattat tgacggtatt
     5941 taccacgtta tgtgtgtgaa agcgtaacca ctattccgct gaaggcgtaa ttgtttaaat
     6001 aacattacgc cgcctggcct taggcctttt gagtatggca acgttttcat aaaaattgct
     6061 gcaaacaaaa atgtcatact t
//
LOCUS       DAZRUF010000233         6048 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZRUF010000233 DAZRUF010000000
VERSION     DAZRUF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.234"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHXOL_003611"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHXOL_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR6810495.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHXOL_003612"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHXOL_003612"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR6810496.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHXOL_003613"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHXOL_003613"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR6810497.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHXOL_003614"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHXOL_003614"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR6810498.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHXOL_003615"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHXOL_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR6810499.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1430 a         1528 c         1645 g         1445 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttgc
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catatttgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgc
//
LOCUS       DAZRUF010000234         6020 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZRUF010000234 DAZRUF010000000
VERSION     DAZRUF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.235"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            114..560
                     /locus_tag="ACHXOL_003616"
     CDS             114..560
                     /locus_tag="ACHXOL_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR6810500.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            571..2022
                     /locus_tag="ACHXOL_003617"
     CDS             571..2022
                     /locus_tag="ACHXOL_003617"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR6810501.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLVLNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2012..3082
                     /locus_tag="ACHXOL_003618"
     CDS             2012..3082
                     /locus_tag="ACHXOL_003618"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR6810502.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3082..4830
                     /locus_tag="ACHXOL_003619"
     CDS             3082..4830
                     /locus_tag="ACHXOL_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR6810503.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4880..5920)
                     /locus_tag="ACHXOL_003620"
     CDS             complement(4880..5920)
                     /locus_tag="ACHXOL_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR6810504.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
BASE COUNT         1418 a         1508 c         1520 g         1574 t
ORIGIN      
        1 atctgacctt tcactctgtc tcaaattgtc attgtttacc gacatatcgc ctctcatcac
       61 ttaatcgctc caccattaca attgattaaa atatatttta tggagtccga ttaatggcag
      121 ctcttactgc aagctgtatt gacctgaata ttcagggcaa tggcgcttat tccgttctga
      181 agcagttggc gacaatagcg ttacaaaacg gttttatcac cgactcacac cagttcctgc
      241 aaaccctgct gctgcgcgaa aaaatgcact ctactggatt tggttccggt gtcgccgtgc
      301 cgcacggtaa aagcgcctgc gttaaacaac cgtttgtatt attcgcccgc aaagcgcagg
      361 ctattgactg gaaagccagc gatggcgaag acgtcaattg ctggatctgc ctcggcgtgc
      421 cgcaaagcgg cgaagaggat caggtcaaaa tcatcggcac actgtgtcgc aaaattatcc
      481 accaggattt tattcatcaa ctgcaacagg gcgacgccga ccaggtgctt gccttgttaa
      541 atcaaaccct cagctcataa ggaagtggcg atggagtcat ccttatgtat tgtcgcgatc
      601 accaactgcc ccgccgggat cgctcacacc tacatggtgg cggaagccct ggaacagaaa
      661 gcccgttctc tcggccatac cataaaagtg gaaactcaag ggtccagtgg cgttgaaaac
      721 cgcttatcca gcgaagagat tgccgctgcc gattacgtca ttctcgctac cgggcgtggc
      781 ctgagcggtg atgatcgcgc gcggtttgcc gggaagaaag tttatgagat tgccatctcc
      841 caggcgttga aaaatatcga ccagattttc agcgaattac cgacaaactc gcagcttttt
      901 gccgcagata gcggcgtgaa gcttggcaaa caggaagtgc aaagcggcag cgtgatgagc
      961 cacctgatgg cgggcgtctc tgcggcgctg ccgtttgtca tcggcggtgg tatcctggtg
     1021 gcgcttgcca acatgctggt gcagttcggt ttgccgtata ccgatatgtc gaaaggcgcg
     1081 ccgtcgttta cctgggtagt tgaatccatc ggttaccttg gcttcacctt tatgatcccc
     1141 atcatgggcg cttatattgc ctcgtcgatt gccgataaac cggctttcgc tccggcgttt
     1201 ctggtttgct atctggcgaa cgacaaagcg ctgctcggca cccagtcggg tgcgggattc
     1261 ctcggcgcgg tggtgctggg gctggcgatt ggctatttcg tcttctggtt ccgcaaagtg
     1321 cgccttggca aagcattaca accgctgctc ggttcaatgc tgatcccgtt cgttactctg
     1381 ctggttttcg gcgtgctcac ttactacgtt atcgggccgg tgatgtccga cctcatgggc
     1441 gggctgctcc acttcctgaa taccattcct ccatcaatga agtttgcggc agcgtttctg
     1501 gtcggtgcga tgctggcgtt cgatatgggc ggccccatca ataaaaccgc ctggttcttc
     1561 tgcttctcac tgctggaaaa acacatttat gactggtacg ccatcgtcgg cgttgtcgcg
     1621 ttgatgccgc ccgttgcggc gggtcttgcg acgttcattg cgcccaaact gtttacccga
     1681 caggaaaaag aggctgccag tagtgccatt gtggtcggtg ccacagtcgc aaccgaaccg
     1741 gctatacctt acgctctggc agctccgttg ccgatgatta ccgccaatac gctggcgggt
     1801 ggcattaccg gcgtgctggt gatcgctttc ggaatcaaac gtctggcacc tggtttgggt
     1861 atctttgacc cgctgatcgg cctgatgtcg ccggtgggtt cgttttatct ggtgctagcg
     1921 attggcctcg tgctgaacat ttcattcatt atcgtcctga aaggattgtg gttacgccgc
     1981 aaagcgaaag ccgcgcaaca ggagcttgtc catgaacatt gagttactgc aacagttgtg
     2041 cgaagccagc gccgtcagcg gcgatgaaca ggaagttcgc gacattctga taaacacgct
     2101 ggaaccttgc gttaatgaga tcacctttga tggtctgggc agctttgttg cccgtaaggg
     2161 gaataaaggt ccaaaagttg ccgttgtcgg gcatatggat gaagtcggct ttatggtcac
     2221 ccacatcgac gagagcggtt ttctgcgctt taccaccatt ggcggctggt ggaatcagtc
     2281 aatgctcaac caccgggtaa ccatacgcac acacaaggga tttaaaatcc ctggtgtgat
     2341 tggttccgtc gcacctcatg cgttaacgga aaagcaaaag caacagccgc tgtcatttga
     2401 tgagatgttc attgatattg gcgcgaacag tcgcgatgag gtggaaaaac gcggtgtggc
     2461 gatgggcgat tttattagcc cggaagccaa ttttgcctgc tggggcgaag ataaagtggt
     2521 cggcaaggcg ctggataacc gcatcggctg cgcaatgatg gctgaactat tgcagacggt
     2581 gaataatccc gaaattacgc tgtatggcgt cggcagcgtg gaagaagaag ttgggctacg
     2641 cggggcacaa acctcggcgg aacacattaa accggatgtg gtgattgtgc tggataccgc
     2701 tgtcgcaggt gatgttccgg gcattgataa cattaaatac ccgctgaaac tgggccaggg
     2761 gccggggctg atgctgtttg acaagcgcta cttccccaac cagaaactgg tggcggcgtt
     2821 aaaaagctgt gccgcacata acgatttacc gctgcaattt tccaccatga aaaccggtgc
     2881 gacggatggc gggcgctaca acgtgatggg cggcgggcgt ccggttgttg cgctgtgcct
     2941 gccaacccgc tatctgcacg ctaacagcgg catgatttca aaagccgatt acgatgctct
     3001 gctcacgttg atacgggatt ttctgaccac cttaaccgcg gagaaagtca acgcgtttag
     3061 ccagttccgt caggtggatt aatgctaaac gaacgccagt taaagattgt cgatctgctg
     3121 gagcaacagc cgcgcacgcc tggtgagctg gcgcaacaga ctggcgtttc aggcaggacc
     3181 atcctgcgtg atattgacta tctcaacttt acccttaacg gcaaagcccg cattttcgcc
     3241 agtggcagtg cgggctatca gctggaaatc ttcgagcgcc gcagcttttt tcagttgctg
     3301 caaaagcacg ataacgacga tcggctgctg gcgctgttat tactgaatac ctttactccc
     3361 cgtgcgcaac tcgcctcggc gcttaatttg ccagaaacct gggtagcaga gcgtctgccc
     3421 cggttaaaac agcgttatga acgcacttgt tgcctggcca gtcgccccgg tttgggccat
     3481 ttcattgatg agacagaaga gaaacgcgtt atcttgctgg cgaacttgct gcgcaaagat
     3541 ccgtttttaa ttccgctggc gggcgtaaca cgagacaatt ttcagcattt atccacggcc
     3601 tgcgacaacc aacaccgctg gccgctcatg cagggtgatt atctctccag cctgattctg
     3661 gcgatttacg ccctgcgtaa tcaactgacc gatgagtggc cgcaatatcc cggtaacgag
     3721 ataaaacaaa tcgttgaaca gagcggcatg tttcttggtg ataacgctgt aagaaccctg
     3781 acaggtttga tagagaaaca gcatcagcaa gcgcaggtaa tttcagccga tcatgtgctg
     3841 gggttgctgc aaagggtgcc aggcatcgcg tcattgaata ttattgatac gcagctggtt
     3901 gagaatatta ccgggcattt attacgttgc cttgccgcac cggtgtggat tgccgagcac
     3961 cgccaaagca gcatgaataa cctgaaagcc gcctggcctg cggcctttga tatgagtctg
     4021 cactttatta cgctactgcg tgaacagctc gatattcccc ttttcgatag cgatctgatc
     4081 ggtttgtatt ttgcctgtgc gctggaacgg catcaaaacg aacgccagcc gatcattttg
     4141 ctctcggacc agaacgcgat tgccactatt aatcagctcg ccattgagcg cgatgtttta
     4201 cattgtcggg tgattattgc ccgtagctta agcgagctta ttgccattcg cgaagagatt
     4261 gagccgttat tgatcattaa caacagccat tatttactgg atgacgcggt aaataattac
     4321 atcaccgtaa aaaatatcat tacggctgcc gatatcgaac aaataaaaca ctttctggcg
     4381 acggcattta ttcgccaaca gccggaacgc tttttctctg cccccggaag ttttcattat
     4441 tcgaatgtac gcggtgaaag ctggcaacat attacccggc aaatttgtgc gcaattagtg
     4501 gcacaacacc atattaccgc cgatgaagcg caacggatca tcgcccgcga aggcgaaggt
     4561 gaaaacctga ttgttaatcg cctcgccatc ccacattgct ggagcgaaca ggagcgacgt
     4621 tttcgtggat tttttattac cctcgcccaa ccagttgagg tgaataacga agtcattaac
     4681 catgtcttga tcgcctgcgc cgccgccgat gcgcgtcatg agctaaaaat atttagctat
     4741 ctggcaagcg tattgtgtca gcatccggca gaagttattg ccgggttaac aggatatgag
     4801 gcatttatgg agttacttca caaggggtga actgtgtcgg atgcgccgca tccgacactg
     4861 catttatcat caaaaacgtt taacgcaggc tattatagtc atcaaccatt tcgttgtact
     4921 cgcgtaacgc tcgtggaaat gagtcttcga ccatccagct tgtgtttgca tcctgaagat
     4981 gctctttatc ccagtcagag tacgggactt tatctctgac gcggcgaaca tattttttag
     5041 tctcgagttg atactggtct gccgaattaa tgaacgagaa attcataccg cctttatccg
     5101 cttctttggc ctgcgctacc agcgtttcca gttcagagac tttcttcatt gccgcatcaa
     5161 catcaaactt ctcttgcgaa atcaggttat taatttgttt cgctgaaatc atgactgcca
     5221 gagagtagta gtggaatgtt ttcccttctc tttcttcaat atttttcagt tccgcaagct
     5281 ggcgcttatc attaatttcc tgaatcgccg catgataaga ttctgctaca ggttcaaagg
     5341 attccagatt ttttaagaat gtctggtgca gcgttttccc tttcgcaaac gcatcgtctt
     5401 tataattttc ctgggtgtaa tatttatcca tttcgttaat ggtattaccc aacgccacgg
     5461 cagcatcaat ataactcacc gccacgccat caataggttg cagcgcaggc gttaacgcca
     5521 cagcactttt tacgcctttt tcacactcag cgaggttgga ttcactaatg ccgtagatgc
     5581 cataaacagt actctcttta ccggtaggcc cctgtttaaa atctttcagc cagtcagcat
     5641 aacgcgccag actgttctgt acagggatct gtaacttgtt gtagcacttg atatagacat
     5701 ccattttttc gtcgaaaacc gcttgttcat cgaccaccgg ttgcgcaggt gtttcaggtt
     5761 tagcttctgc ttttgcaacg ggagcttcag gagctggtgc ggcaggttga ctattggcag
     5821 gcgggggcgt ttctgtttcg gcttttttat catcacaacc cgtcagaccg agggccatga
     5881 tggcgactat aatagcggaa gataataaat tccttttcat ttaattgcct tataaatata
     5941 tagataagag agtgaacata ataaatgaaa aaagaaaacg tcactacacg cgttttattt
     6001 ataaataaaa taataaaatt
//
LOCUS       DAZRUF010000235         5935 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZRUF010000235 DAZRUF010000000
VERSION     DAZRUF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.236"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            293..369
                     /locus_tag="ACHXOL_003621"
     tRNA            293..369
                     /locus_tag="ACHXOL_003621"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:327..329,aa:Arg,seq:tct)
     gene            complement(414..1175)
                     /gene="envY"
                     /locus_tag="ACHXOL_003622"
     CDS             complement(414..1175)
                     /gene="envY"
                     /locus_tag="ACHXOL_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR6810505.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1358..2248)
                     /locus_tag="ACHXOL_003623"
     CDS             complement(1358..2248)
                     /locus_tag="ACHXOL_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR6810506.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYIRAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2249..5221)
                     /gene="nfrA"
                     /locus_tag="ACHXOL_003624"
     CDS             complement(2249..5221)
                     /gene="nfrA"
                     /locus_tag="ACHXOL_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR6810507.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5208..>5935)
                     /gene="nfrB"
                     /locus_tag="ACHXOL_003625"
     CDS             complement(5208..>5935)
                     /gene="nfrB"
                     /locus_tag="ACHXOL_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HFR6810508.1"
                     /translation="LDTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESI
                     DAWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVR
                     YFIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFA
                     EILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQ
                     SLLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1347 a         1560 c         1550 g         1478 t
ORIGIN      
        1 attctgatga taggatgagt atccataatg atcaccgacg ttggtttcat agttaccagt
       61 ctcataggag cggacaattt tccgttaggg cggtaaattg tactttgata catgaaaata
      121 cgggttttct tgattcaaac gcgcagcggt gtgcatttgt ttgccgctat agcgtaataa
      181 atcagaaaat cagacgcggt cgttcacttg ttcagcaacc agatcaaaag ccattgactc
      241 agcaagggtt gaccgtataa ttcacgcgat tacaccgcat tgcggtatca acgcgccctt
      301 agctcagttg gatagagcaa cgaccttcta agtcgtgggc cgcaggttcg aatcctgcag
      361 ggcgcgccat ttcttcttca tttaaccccg tcttatccgt tccgctttgc tcttcaccac
      421 atcacttttt gtcgctgttt ggcgagataa ttcaacggtg tcaggccgta aaacgcctta
      481 aaaacagaga taaagtacga cgtgctgcta tagccacata attgcgccac ctgagtgata
      541 tttttgttat ccatcaataa catctgtacg gcgtaacgca tacgacactc tgtgacaatc
      601 tggctatagc tgatattttc gttttttaat ttctttttga gcaggctggg gcttaaacat
      661 agcgaactgg cgacaattcg cagattccag taatgctgaa tatcgctttg aataatgcgg
      721 cagacgctgt cgcggacgct gctgcgtaag atatacatca gtagggcaat aaaccgcgat
      781 tgctcaagaa agttagacaa tacggtaaaa agcaatgcgc gcgtcaactc cgcttcgcag
      841 ggtttgctgg gttgctgctt gctgtggttg gcggctagcc ggaacacttc cggtgtacgg
      901 ctatgacaag cgataaccgg ggttgccagc cgcgaccagg gctttacaca ggttaagttc
      961 ttatttaaaa acaacagata atcgttgatg atgttacggt tgatgtgggt gattaatacg
     1021 tgatttaacg ttgagagttc aatgacgtta ttgttgcacg cgaaggcggc caggtagttt
     1081 ttcggaaggg taaacgtagc atggttattg acgcttacct ctacctcttt ttcggtcagg
     1141 atcaccacgc aaggttcact gctgctcaat tgcatttcgc actcctcaga tatcagaaac
     1201 tccgctcaaa ggatctatgc ttcctgcatg agtgatcggc ccgttcgccg ataacgatct
     1261 tctttcttta gcacgctttt tagcaattaa tcttgatgga attctgacga gagcgaaaga
     1321 ggtaagccag gtcgtacccg acttacctgg aggagattta atactcgaga atgccgtgcg
     1381 cgacgggcaa atagcgcaga gagaaataga gagtgtcttt accgcaagag gaacgtttcg
     1441 ctaacagcga ggcgatctct gcatccggtt tcggtttcgc tgtaaaggtt ttgcctttgc
     1501 cggcgacaaa cagttcataa acaatgccgc ttgctggggc ggagctttgg caatcggcgc
     1561 tggcctgtag ataacgttca cgctgttcag cggtaagttt atccacgccg ctgccatcct
     1621 gtacccagac attaactccg gttgctttaa cttgttccag cagttggcga tagccatctg
     1681 gcgacatgtt tccggcgaaa aaactactga tataaaccgg ttttgctgaa acatcgctaa
     1741 tcagccgctg cgcgttgttt aaccatgtta gcaaaggctg acgggcagct tcgctgcgcc
     1801 agttcaggtc gtcaatttcc gccctgatgt accagccatc cggcgttatg ccaggcgcgg
     1861 cgctccataa tctggcttgc tggagatcgg cagccagcag gcgattaaga tagctttcca
     1921 gcgctgcgga cgactgtttc tggtgcataa aaaattccgg atcggcgttc agcccgacaa
     1981 tcagtttcag gccagcctgt tgcgcagctg cggcacgctt aaacaataac gtgcgctgtt
     2041 ctggctgggt aaatgcatcg ccgtaacggg tccattgcaa aaccagggta tcgaagcctt
     2101 gcaaacgtaa ctgactcatc agcccctgcc actgggtatc ggtaacctga ctatctcggt
     2161 tttgtggttg ccagataata cctttcatcg caaaggaaaa agggctgacc aaaagcagtg
     2221 tcagcaatac gaaaatgaac ttacgcattt accagtgcac tccaatggtg agaaacgcgt
     2281 tgttgcgctc tccgttacgt tgattaatcg ccttaaaggt atgttgatac tcgacgccga
     2341 gactgacttt gtgcggccag gcgtcgtagt gcgtctcgcc ggtccagata ttccagcgga
     2401 ccccgactcc gccaagctgc gcgccctgag tgcctttatc acgatagccg ttgtcctgaa
     2461 cgtgagcgta aggctcaata gtctgtccgt tagctacctt ctgatgccag ctgacgcgat
     2521 aatctgccgt ccacgcctga atatcctggc ggatatattg cgccgcatcg aggtacaggt
     2581 tttgggcaaa ccagcctgaa ccgttcgggt gccattcgtc gctgtatttg ccgccattaa
     2641 agaatgaggc gctggcgcgc agcatggtat cggatgcgcc attttgcccg ttcagcggca
     2701 actgctgttc gacggcgagg aaaaagatct gatcgcgcag cggcttccag cgcagaccgg
     2761 tgccggacat cggatttttc accggcatca tcaccccgtt ttctccggta tcggcaaaga
     2821 cgcggctata aactgagagc aggtcgcctt ccagcagcat attgcgtccg atgcggtact
     2881 cggcttccag ttgcccgtag ctacgatagc ttttccctgg cgctgcgccg ccaacattat
     2941 tgttagcggt actcattgcc ccggaacgca agccgataga agaatcgaaa ctgaacgtcc
     3001 agcggcgacc gacctcctca tgcaaacggc ggaaattgaa gcgttgttga ttttgctctg
     3061 gggtcagtgg ggttatcagc gcctgattat caatgtcatc aatcaccagc cgggcgtagt
     3121 gctgcgtcgc aggcatgtca tccagacgct ggttcacgta ggccagttgt cggatcagtg
     3181 ccggatcgtc cggaagcctt ttatgcgcct gttcgagcat ttcccgcgac tgtgcgatat
     3241 caccgctatc ccacaaggcg taaccaagcg ctgcctggat gttgctatta ttcggttcca
     3301 gttccagcgc ggcgcgcaaa tcactcaccg cggccgggac attatgacgt tggcgataaa
     3361 ttgtcgcccg cgcaacgtaa gcgttggcag aaggcgcaat attgattgag cgcgtgagat
     3421 cgttcagtgc gagttccggc tgaccaggaa tgtaacgttg tgcatgcagc caccagtaga
     3481 gggcattgct tcccagtcca cgttgttctg cctgttgtag ccagcgatcg cgagccgcac
     3541 catttcctgc cgcctgggcg gtattggcag cagcaagcag atcctcattg ctcatgtcgt
     3601 gaagactgat tttctgccag gccgccagtg cggtggcgta gtcctcaacc tgatacgcct
     3661 gataggctac cgcacgatgt tgccaggcgc tcggttgtcg ttgttcggcc tgaagccatg
     3721 catacaacgc cacaccgggt agcgtgtccc gataacactt tgccagacgg ttccaggcgg
     3781 cagcatcgta ggaaggcgac atatcgccca gcaagcgaac tattgccggg caattatctg
     3841 caatacccgg caactgactt tgccactgac gttgctccgc cagcggtaag ggtttcgata
     3901 aaatcgccac cttcgccggc gttgccaggt aaggatgact ttccagcaga gacgccagtc
     3961 gcgccattaa agtctggctg acacgcgcat cgccctggaa aggatagcgt tgcagcaata
     4021 aatcggcagc ttcgcgtgac tgctcgttct gcatcagttg ccaggtcagt tgatccaggc
     4081 gggtaagatt tgccggttct tgctgataca gcaatcgtgc cagacgcaga gattcagcct
     4141 tgttacgggt cgccacgctg acagcataac gctcctcaag catttcattg gcggggaggg
     4201 tggcgagcag tttttgcgct gcgtcgtact gaccttcttt taacagcacc ggtagcgtcg
     4261 cgccaacaac atactggcgg ttatcggcaa actgcaccgt ataattcgcc aatgcctgaa
     4321 cggggttggc gctgtattta gataacagat agagccaact tttctcttgt gcgtccgtgg
     4381 taaacagcgg cttattttca ttgagataat gctggaggcg tgctttttcg ccacgataag
     4441 ccagcgcggt cgcgtaagta atatatgact gaggatcggt gaagatcccc tgtgattgca
     4501 gtgctaggat ccgatcgtcc agctgcccgg caagaagcac gtcaaaccac tgacggcgtt
     4561 ctgccgcgct taatgtgttc tgctggcgtg cttcattgta tagcgtatct gcctgggacc
     4621 attgtttcag gtagattgcc cgttgcagca gatcggttcg cagcgttttt ccttccggcg
     4681 atgcagcaaa cgtcgcatcg ttcagttgcg ctctggcgac aggtaactgt gcaagccgca
     4741 gggcattctg cccgacttca ctgcgacaac gcagggtcgg tgcagcatcg cacgcttttt
     4801 gctgggcaag cagttcttca acagttgtaa cgcttttcac ttcaaccgga atagccgcca
     4861 gactgcgctc aagtcgggca tctcctgggt gacgtttcag ttgatcctca agcaacagcc
     4921 gcgcccggtc atcatgacca aaatggcgat aggcttccgc aaggtataaa gtcagcggaa
     4981 tattatccgg cacctgctgg tgtatatatt caaattcgcg gatggcggtt gcttcgtcgt
     5041 tatttttctg tgccttcagc gccttatcga gacggggata aataacaaaa tggcgataat
     5101 cgctcagccc cagctcttct gcgctggtgc cgatattgtc tgcgagtgcg ctggtactca
     5161 ataaagacgt cagcagtaaa ccagaccatc cgatgacgcg attaaggtta ttctccttca
     5221 ttttcggact ccagttgcgc aacctgttct gtgtttaaac ctgctttgag taatagtgat
     5281 tgcatcgaaa cttgtaattc gcgttgaatt gtcaggacgc gatccaacgt ttcctggctg
     5341 ataacgcctt cggtgaccaa aaacttgccg agcggcagag aactgcgttc atggcgcaat
     5401 aacaacacgt taattgctga acgattaata tgaccgagcg tggtcagtat ttcggcgaac
     5461 aggaactgat gcggcacata ttgccgccag atttcaccgg cctgctgttc cgtgagccac
     5521 tgatgctgaa ccgcattgta caacattgcc cgcggatcgt gaccgcgtcg gcgtgcatac
     5581 cagtgacgta accccgtgac aatttgtccc cgcagaacaa tgaagtaacg cactttgcgt
     5641 ccgactttac gcgtcagggc cgccagcgaa accgggtcaa taccatcttc actgccgaca
     5701 attaactcat cattttccag acgcagcggc agtaccgcat aatgcagcgc cacggaggcc
     5761 ggcatttcgg caatcagcga ggaagggatc tgccaggcat cgatggattc ccacgccacg
     5821 ccgttttgct ctgccagcgc ctgtgccagc tgctcggcgc taatcagccc ctgcatcagc
     5881 attgaaccgc ccaagcgtag accttcgacg cgattacgca gtgctgtatc gagtt
//
LOCUS       DAZRUF010000236         5852 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZRUF010000236 DAZRUF010000000
VERSION     DAZRUF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.237"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(39..401)
                     /locus_tag="ACHXOL_003626"
     CDS             complement(39..401)
                     /locus_tag="ACHXOL_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR6810509.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            938..2851
                     /gene="mtlA"
                     /locus_tag="ACHXOL_003627"
     CDS             938..2851
                     /gene="mtlA"
                     /locus_tag="ACHXOL_003627"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR6810510.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3081..4229
                     /gene="mtlD"
                     /locus_tag="ACHXOL_003628"
     CDS             3081..4229
                     /gene="mtlD"
                     /locus_tag="ACHXOL_003628"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR6810511.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4229..4816
                     /gene="mtlR"
                     /locus_tag="ACHXOL_003629"
     CDS             4229..4816
                     /gene="mtlR"
                     /locus_tag="ACHXOL_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR6810512.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4828..5037)
                     /gene="yibT"
                     /locus_tag="ACHXOL_003630"
     CDS             complement(4828..5037)
                     /gene="yibT"
                     /locus_tag="ACHXOL_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR6810513.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5322..5684
                     /locus_tag="ACHXOL_003631"
     CDS             5322..5684
                     /locus_tag="ACHXOL_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR6810514.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1474 a         1449 c         1519 g         1410 t
ORIGIN      
        1 cagcaccacg taagttaaaa caatcaatag atccataatt agatctccgg gaacgtggaa
       61 tagtcagtct ttttctcggc ggcgagttga tgctctagcc gggaaattcg gtcagaaagt
      121 gcggcgattt ccgggtcagt cgctttctcc tgcgacgcaa catgtgactg catgccccac
      181 ccgcgctccg gttgatagag cgtcgcccag atccacagga acggccagat aacatgcagg
      241 gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg catggggatg gttgcgcttt
      301 ttggcaatca gataagggat gtcatgtatc gcgatgattc cataaaaaat caccagaaag
      361 acgaagatca gcactcccaa cgcgaaatag tttagaaaca tatctgcctc ggattcacgt
      421 ttatcagtgt tgtttttggg ctggcagcca gaagggagtc aggctgatat tttgacaata
      481 atccgggttc gcgattctcg ccataacacc aaagaataat ttttagaggt gatgagttgc
      541 ttagttacat aacaattgta tgacgaaggc ataacatgct gtagatcaca tcaggtgaac
      601 gccgtaagaa aatatcttgt gattcagatc acaaagattc aacaaaccat caaaacaaaa
      661 atgtgacact actcacattt aaatgccatt tttagcgaaa atcgccgcct tgttgctttt
      721 ttacacaagc gttttgtgat gaacgtcacg ttaattacct ctctaccccc tatatttatg
      781 tgattgatat cacacaaaag gccgtcgact ggacagttaa ccgattcagt gccagatttc
      841 gcagtatcta caaggtccgg ctacctctgc cgccacatta acaaaaaacc tcgggcttcc
      901 agcctgcgcg acagcaaaca taagaagggg tgtttttatg tcatccgata ttaagatcaa
      961 agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat
     1021 cgcgtggggt atcatcaccg cgttatttat tccaacaggg tggttaccga acgagacgct
     1081 ggcgaagctg gtcgggccga tgatcactta tctcctgccg ctgctgatcg gttataccgg
     1141 tggtaagctg gtaggcggcg aacgtggcgg cgtagtcggt gccatcacca ccatgggcgt
     1201 tatcgtcggc gcagacatgc cgatgttcct cggttctatg attgcaggtc cgctgggcgg
     1261 ctggtgcatt aagcacttcg accgctgggt agacggtaag atcaaatccg gttttgagat
     1321 gctggtgaat aacttctccg caggcatcat cgggatgatc ctcgctattc tggcattcct
     1381 cggcattggc ccgattgttg aagccctgtc caaaatgctg gctgcgggcg ttaacttcat
     1441 ggttgtccat gacatgctgc cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt
     1501 cctcaacaac gccattaacc acggtatctt ctcgccgctg ggtattcagc agtcccatga
     1561 actgggtaaa tcaatcttct tcctgattga agctaaccca ggtccaggta tgggcgtgct
     1621 gctggcgtac atgttctttg gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat
     1681 catccacttc ctgggtggta tccacgaaat ctacttcccg tatgtgctga tgaatccgcg
     1741 tctgatcctc gcagtcatcc tcggcggtat gactggcgtg ttcacgctga ctatcctggg
     1801 cggtggtctg gtttctccgg catctccggg ttctatcctt gctgtactgg cgatgacacc
     1861 aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt
     1921 tgtctctgct attttgctga aaaccagcaa agtgaaagaa gaagatgata ttgaagcagc
     1981 aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc acatctccgc tgtctgcggg
     2041 cgatgtgact aacgacctga gccacgtacg taaaatcatc gttgcctgtg acgccggtat
     2101 gggttccagt gcgatgggcg caggcgtgct gcgtaagaaa attcaggatg caggtctgtc
     2161 acagatttcg gtcactaaca gcgcgatcaa caacctgccg ccagatgtgg acctcgtcat
     2221 cactcaccgt gacctgaccg aacgcgctat gcgccaggtt ccgcaggcgc agcatatttc
     2281 gctgaccaac ttcctcgaca gcggcctgta caccagccta accgaacgtc tggttgctgc
     2341 ccagcgccat actgaaaacg aagtgaaagt gaaagacagt ctgaaagaca gctttgacga
     2401 ttccagtgct aacctgttca aactgggcgc ggagaacatc ttcctcggtc gcaaagcagc
     2461 aaccaaagaa gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga
     2521 gccggaatac gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga
     2581 gtctatcgcg gtgccacacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt
     2641 ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg
     2701 tctggtgatt ggtattgcag cccgtaacaa cgagcacatt caggttatca ccagcctgac
     2761 caatgcactg gatgatgagt ccgtcatcga gcgtctggca cacaccacca gcgtggatga
     2821 agtgctggaa ctgttggcag gtcgtaagta atccaatccc accctctcca catggagaag
     2881 gtggggttaa ttgcctgatg cgctacgctt atcaggccta caggatgcat cacgatttgt
     2941 tgaatttgca cgttcttgta ggccggataa ggcgcttacg ccgcatccgg cgctgcccct
     3001 ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa
     3061 cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg
     3121 gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc
     3181 aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg gttggtgaaa
     3241 ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt gatgatgtcg
     3301 ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt gtgctggaac
     3361 gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc
     3421 cgctgaacat catcgcctgt gaaaacatgg tacgcggtac cacgcagctg aaaggccatg
     3481 tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agaacacgtt ggctttgtcg
     3541 attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat ccgctggaag
     3601 tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc
     3661 cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct
     3721 tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga
     3781 ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag
     3841 aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca
     3901 tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag
     3961 gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ttgctcggta
     4021 cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt gcaatgcact
     4081 tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc
     4141 cgcaggcggc gctggcacag atttccgatc ttgatgccaa cagcgaggtt gtatccgagg
     4201 cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc
     4261 gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg
     4321 tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga
     4381 gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc
     4441 ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt
     4501 gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga
     4561 gctgctgatg gcattgcgtg aagaactaaa tcacgacggc aacgagtacg cctttaccga
     4621 cgacgagatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc
     4681 acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc
     4741 tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccagaatca gcttaaaaaa
     4801 agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag
     4861 ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga
     4921 cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc
     4981 catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat
     5041 gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt ttaggttccc
     5101 ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgtttat
     5161 tatctttttt ctatagattt cttaattaat caataaattg cgtagttttt aagcatgtta
     5221 atgattacgt tacagaacta ttgcaatgtc atcagttgtg aacaaagcac ctggtcgcgc
     5281 atactagggg ctataaattt atctttatca gaagccatca catgaaagaa gtcgaaaaaa
     5341 acgaaatcaa acgtctcagc gatcgtctgg acgccatccg ccaccagcag gccgatctgt
     5401 cgctggttga agccgcagac aaatatgccg agctggaaaa agagaaagcc acgctggaag
     5461 cagaaattgc tcgcctgcgt gaagttcata gccaaaaact gagtaaagaa gcacagaagc
     5521 tgatgaagat gccgtttcag cgcgcaatta ccaaaaaaga gcaggctgat atgggcaagc
     5581 tgaagaaaag tgttcgcgga ctggtggttg tgcacccaat gaccgcgctg ggccgtgaaa
     5641 tgggcctgca ggagatgact ggattctcta agactgcgtt ttaagcgcct cagacgactg
     5701 gttcgatcat atcaacccgc atctggcttg ccttagctgg tcaatgcggg gactctcctg
     5761 tctactcgcc taattaccaa acaattcact ccctttacgt cgatttaccg ctggttgata
     5821 ttctgaaaat gcatttttac cgaaatagcc ac
//
LOCUS       DAZRUF010000237         5670 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZRUF010000237 DAZRUF010000000
VERSION     DAZRUF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.238"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..663)
                     /locus_tag="ACHXOL_003632"
     CDS             complement(<1..663)
                     /locus_tag="ACHXOL_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR6810515.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     ILD"
     gene            763..1353
                     /locus_tag="ACHXOL_003633"
     CDS             763..1353
                     /locus_tag="ACHXOL_003633"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFR6810516.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPVWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(1435..2175)
                     /gene="pflA"
                     /locus_tag="ACHXOL_003634"
     CDS             complement(1435..2175)
                     /gene="pflA"
                     /locus_tag="ACHXOL_003634"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR6810517.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(2367..4649)
                     /gene="pflB"
                     /locus_tag="ACHXOL_003635"
     CDS             complement(2367..4649)
                     /gene="pflB"
                     /locus_tag="ACHXOL_003635"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR6810518.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(4704..5561)
                     /gene="focA"
                     /locus_tag="ACHXOL_003636"
     CDS             complement(4704..5561)
                     /gene="focA"
                     /locus_tag="ACHXOL_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR6810519.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         1461 a         1335 c         1412 g         1462 t
ORIGIN      
        1 atccagaatg atattccctg cgacagcaat ttcctgctcc tttgcaaatt gccagtggaa
       61 aggtttgccg ctgggataac gaaatacaac acattgatga ttaagcagat catgaggatg
      121 gcggggtttg ccatatcttg caaagtactc tggcgttgct gctacacata atttcactgg
      181 tgggccgatc gccacgctga tcatatcttt ttcgacgatg ccactaagtc gaacgccagc
      241 gtcaaagccc tgttggacga tatcgacaag agagtcgtca gttgtgagct cgactttaat
      301 atcaggatat tcacgtgtga aaccaaccag taaaggcatt aaaaatatac gtgcagctac
      361 ccgggcggca ttgattttga gcgttcctgt tggtgtcaga cgaaaatcat tcatctcatc
      421 taacatgatc tgaatttcgt cgaaagctgg gcgcaatcgt tcatagagat tagaacccgc
      481 ttccgttaac gatacgcttc gggttgtccg attgaataag cgaattttaa gacgttgttc
      541 cagtgtttta atactatggc taatggcgga cgaggataag cctaactcat cgcccgctgc
      601 acgaaagctt tgattacggg ccacagcaaa gaaagtggca aagtcagaca tattcatccg
      661 cattagtgaa aatccttcat taactcatcc tgaatcatca cgtttacatg attatgccgc
      721 ctgcgtaaag tgcgctccag aacttaacgt ggaggtaaaa ttatgcagtc tgaacgtatt
      781 tatttggtat gggcccatcc tcgtcatgat tcattgaccg cacatattgc tgatgcgatc
      841 catcagcggg caatggagcg gaaaatacag gtgacggaac tcgatttata tcggcgtaat
      901 ttcaacccgg tgatgacgcc ggaagatgaa ccagactgga agaatatgga taaacgttat
      961 tctccagagg ttcatcagct ttattcagag ctgcttgaac atgacacgtt agtggtggtt
     1021 tttcctgtct ggtggtacag cttcccggca atgctaaaag gatatattga cagagtatgg
     1081 aataatgggc tggcttatgg agatgggcag aaattaccat tcaataaagt tcgttgggtg
     1141 gcgctggttg gaggagacaa agaatcattt gtccagatgg gctgggaaaa aaatataagc
     1201 gattatttaa aaaatatgtg cagttatctt ggtattgaag atgccgatgt cactttcttg
     1261 tgtaatacag tggtattcga tggggaagaa cttcacgcga gctattatca gtcgttatta
     1321 tctcaggtac gggatatggt agatgcacta taagatgtgt taaaaacgct gtagcagaat
     1381 gaagtgcgga ataaaaaagc ggcaactcaa taaagttgcc gctttacggg gaaattagaa
     1441 cattacctta tgaccgtact gctcaagaat gcctttcacg cgttccatgg tctctttctt
     1501 cggtggttta acaccgtcga gtttgtactc ttcacccatt gccacccatt tgtgtttgcc
     1561 cagctcgtgg taggggagaa gctcgatttt ctcaacgttg cccatatcac gggtaaattc
     1621 accgaggcga tgcgctgaat cgtcatcgtc agaccagcct gggacaacaa cgtagcggat
     1681 ccacaccttc acatttttgt tcgccagata tttggcgaac tccagcgtgc ggtgattgga
     1741 aactccaacc agattttggt ggatctcgtc gttcatctgt ttgagatcga gcattaccag
     1801 gtcggttact tccagcagtt catcaatcac cggatcgtaa cgacgaacaa aaccgttggt
     1861 gtccagacag gtatgaatgc cttctttttt gcaggcgcgg aaccagtcac gaacaaactc
     1921 agcttgtagg attgcctcac cgccggatgc ggtaacgccg ccgccggaag cgttcataaa
     1981 gtggcgatag gtcaccacct ctttcattaa atcttcaacg gtaacttctt taccgccatg
     2041 cgtatcccag gtgtcgcggt tatgacaata caggcagcgc atcaggcagc cctggaaaaa
     2101 ggtgataaag cgaatacccg ggccgtctac ggttccacag gattcaaagg agtgaatgcg
     2161 accaataact gacattgcgg tgtttctcca gatgtggccc atctgaggcc gtgttggtgc
     2221 gcagctcgaa ggctacgtcg agtctgtttt ggcagtcacc ttagagtata gatagctgac
     2281 aaaaaaggct ctcgtgctaa aaaaggcccc actttcgtgg agcctttatt gtacgctttt
     2341 tactgtacga tttcagtcaa atctaattac atagattgag tgaaggtacg agtaataacg
     2401 tcctgctgct gttctttagt cagcgagttg aaacgtactg cgtagccaga tacacggatg
     2461 gtcagctgcg gatatttttc cgggttttcc atcgcgtcga gcagcatttc acggttcatc
     2521 acgttaacgt tcaggtgctg accaccttcg atggatgctt cgtggtggaa gtaaccatcc
     2581 atcagaccag ccaggttggt cttacgaact tcgtcgtctt tacccagtgc gttcggaacg
     2641 atagagaagg tgtaggagat accatcttta gcgtaagcaa acggcagttt agcaacggaa
     2701 gtcagagagg ctactgcacc tttctggtca cgaccgtgca tcgggttagc acccggtccg
     2761 aacggcgcgc cagcacgacg accgtctggg gtgttacccg ttttcttacc atacacaacg
     2821 ttagaagtga tggtcagaac agactgagtc gggatagcgt cacggtaggt gtgcagtttc
     2881 tgaattttct tcatgaaacg ttctaccagg tcaacagcca ggtcatctac acgcggatcg
     2941 ttgttaccaa actgcgggta ttcgccttcg atttcgaagt cgatagccag accgtcttcg
     3001 tcacgaatcg gtttaacttt cgcatatttg attgcagaca gggagtcagc agcaacggac
     3061 agaccagcga taccacacgc catggtgcgg ataacgtcac ggtcgtgcag cgccatcaga
     3121 gaggcttcgt agctgtactt gtcgtgcatg tagtggatga tgtttagtgc agtgatgtac
     3181 tgtttagcca gccagtccat gaagtgatcc atgcgctcca tcacttcatc atagttcagg
     3241 acatcgcctt tgatcggttc agacttcgga ccaacctgca ttttcagttt ttcgtcaacg
     3301 ccgccgttga ttgcgtacag catggttttc gccaggtttg cacgcgcacc gaagaactgc
     3361 atttgtttac caacgatcat cgggcttacg cagcaagcaa tagcgtagtc atcgttgttg
     3421 aagtccggac gcatcaggtc atcgttctca tactgcagag aagaggtgtc gatggacact
     3481 ttagcggcga atttcttgaa gttcagcggc agtttttcag accacagaat ggtcatgttc
     3541 ggttccggag acggacccat ggtgtacagg gtgttcagga aacggaagct gtttttggta
     3601 accagggtac gaccgtcgag gcccatacca ccgatagatt cggttgccca gatcgggtcg
     3661 ccagagaaca gttcatcgta ttccggagta cgcaggaagc gaaccatacg cagtttcatg
     3721 accaggtggt caaccatttc ctgcgcttct tgttcggtga tcttgccagc tttcaggtca
     3781 cgttcgatgt acacatccag gaaggtggag gtacgaccga aggacattgc agcaccgttc
     3841 tgagacttaa cagcagccag gtagccgaag taagtccact ggatagcttc ctgagcgttg
     3901 gtagccggac cagagatgtc gtagccgtat ttcgcagcca tttctttcat ctgacccaga
     3961 gcgcggtgct gttcagcgat ttcttcgcgc agacggatag tctgttccaa gtttacgccg
     4021 ttttccagat cagcctgcag agaagtgaac tgtgccagtt tgtctttcat caggtagtcg
     4081 ataccgtaca gcgcaacgcg acggtagtca ccgatgatac ggccacggcc atatgcatct
     4141 ggcagaccgg tcagaacacc agatttacgg caacgcagga tgtccggagt gtaaacgtcg
     4201 aacacgccct ggttgtgagt tttacggtat tcagtgaaga tttttttgat catcggatcc
     4261 agttcgcggt tgtacgcttt gcaggaacct tcgatcattt tgataccacc gaacgggata
     4321 agagcacgtt tcagcggagc ttcagtctgc agaccaacga ttttctcaag ctgcttgttg
     4381 atgtagccag cgtcgtgaga ggtgatggtg gaagcaacag cggtgtcaaa gtcaactggc
     4441 gcgtgagtgc ggttttccag tttaacgcct tccattactt tgtcccacag ggtggtggtc
     4501 gcttcagtag cgccagccag gaaggactcg tcaccctcgt acggagtgta gtttttctga
     4561 atgaagtcac ggacgtttac ttcattctgc cagtcacctt tggtaaaacc ttcccaggct
     4621 gtggctaact tttcattaag ctcggacatg taacacctac cttcttaagt ggatttttta
     4681 tttactgcgt acttcgacaa ccattaatgg tggtcgtttt cacgcaggta aatgacccag
     4741 tatgtcaacc caaccaacaa accaccaccg ataatgttgc cgatcgtaac cggaatcagg
     4801 ttatcagtga tgaaattcat cacggtcagg tgagaaaaat tttccggtgc agaaccgact
     4861 gcggtccaaa attccgggga tgcgaagtcg cggattacaa tacccatcgg gatcataaac
     4921 atgtttgcga tactgtgctc aaaaccgctg gcaacaaaca tcgcgaccgg cagcaccata
     4981 atgaacgctt tgtccatcag gctgcggcca gaataactca tccatactgc cagacatacc
     5041 atcaggtttg ccaggatacc aagacagacg gcctcaataa aagtatggtg cactttgtgg
     5101 tcggcggttt gtaggacgtt tagtccccat tgaccatttg cggtcatata ctcgccggaa
     5161 agccacatta aaagtacaaa cagcagtgcg ccgaccaggt tgccaaaata gacatttagc
     5221 cagtttttcg ccaactgacc ccaggtgatg cgcccactcg ccttagcaac aacaatcaac
     5281 acggtggaag taaagagatc ggctccgcag acaacacaaa gaatcagccc cagagagaag
     5341 caaatgccgc caaccagttt tgccatgccg aagggcattg tgcctgtgcc agtggttgct
     5401 gtgatataga agacgaatgc gattgagatg aaaacaccgg cggtaatcgc cagatagaaa
     5461 gtcttaagcg gatgtttcgt tgctttatag acacccgcct cttcggccac tttggccatt
     5521 gcagcaggaa gtaaaagatc aaaagggttg tcagctttca cactaactct ctctttatta
     5581 agtcggcgac gagatactaa caaagcatta tagatgagaa attgatatag atcatatctc
     5641 gcctggctta taggcccgta actcgcatgg
//
LOCUS       DAZRUF010000238         5655 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZRUF010000238 DAZRUF010000000
VERSION     DAZRUF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.239"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(463..1251)
                     /gene="phoH"
                     /locus_tag="ACHXOL_003637"
     CDS             complement(463..1251)
                     /gene="phoH"
                     /locus_tag="ACHXOL_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR6810520.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1872..3143)
                     /gene="efeB"
                     /locus_tag="ACHXOL_003638"
     CDS             complement(1872..3143)
                     /gene="efeB"
                     /locus_tag="ACHXOL_003638"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR6810521.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(3149..4276)
                     /gene="efeO"
                     /locus_tag="ACHXOL_003639"
     CDS             complement(3149..4276)
                     /gene="efeO"
                     /locus_tag="ACHXOL_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR6810522.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4334..5164)
                     /gene="efeU"
                     /locus_tag="ACHXOL_003640"
     CDS             complement(4334..5164)
                     /gene="efeU"
                     /locus_tag="ACHXOL_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR6810523.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         1436 a         1542 c         1321 g         1356 t
ORIGIN      
        1 caaccatccc tatttagcca ttgcggcttg ccttccacaa aaaaataaca acacacaata
       61 ttagcaaaga attaattaag attaacctta catttataat attatcccta aagaataaat
      121 cagaggaata tattcggtgt taaacagtca gtcaaagtat agcccattgt tgattaaacc
      181 tcataagcaa aattggcacc agatctcctg tacaggttga atggccgaaa aaagataggc
      241 agatgctcat ttttttggct tctggaagaa aaactctccg cgctggcagt attacactgg
      301 tctgatgcta tgacgaaaaa tgcctggtaa gagtatctaa accagactcc agttcagctt
      361 atcaacacgc tcaaagtcgc ccccctgaac gctaacctga agtgaaaatc tgttgaaata
      421 aaacaaagcc cggttcgccc gggctctgca ccgataacac acttaactgt aggcatgcag
      481 cgtacgttgg caaagtgccg aacgtacgca gtcctcttta ccgaaccgga cgatcccaac
      541 catttcatct tcttcgaaac gttccagcgc gtcacttaat ccggagcaca cgccgcgagg
      601 caaatcgcat tgcgtgatat caccgttgac gataaccgtc acgttctccc cgaggcgggt
      661 taaaaacatt ttcatttgcg cggcagtcac attctgcgcc tcgtcaagaa tgacgactgc
      721 attttcaaag gtacgtccac gcatataggc gaacggcgca atttccacct tccctatttc
      781 cggtcgcagg cagtactgca taaaggaagc ccctaagcgc cggaccagca cgtcgtagac
      841 cgggcgaaaa tagggagcaa acttttctgc gatatctcca ggtaagaagc caagatcttc
      901 atcggcttgc agaactggac gggtgacgat aatcctgtcg acatccttat gtatcagggc
      961 ctctgccgct tttgctgcgc tgatccaggt ttttccgccc ccggcttcgc ccgtggcgaa
     1021 tatcagctgc ttactctcaa tagccttcag atagtgcaat tgcgcttcat ttcgcgcgag
     1081 gatgggcgaa gtatcgcgac tgtcgcgggc cataccaatg gcttctacgc cgcccatctg
     1141 cacaagcgag gtgaccgatt cttcttcacg ctgcttatgg ctgcgcgaat cccgtctcag
     1201 cacacgtttt gcctcgcgac gagctttgat cactgctttt tgtcttccca tagagagcac
     1261 cttgagttgt ttgtattcat cacacgcgcc gttggcagcg cgattatgcg cacgaacatc
     1321 agagggttgg cttccttgta agccatagtt tgcttttgga taaaatgccg aaaaacggct
     1381 acgcgcaccg tttacggcgt cggtaacaca tgaaaagaaa ggatgaggtt gaaaatgcaa
     1441 agtgacgaga tgactaccgg aggagaaaac tccgcgagtg gtggcgcgtt gattatctaa
     1501 aacatgtcca gtacaggacg ttaccatccg cgatctccat agtgactgac tatcactgcc
     1561 gggaacttcc gctgctactt aataagtaca acagatctcg catttattgc aacaatatat
     1621 ttacttatat ttaactataa aacactattt cagtgacatt agtttctact ggaaagatga
     1681 cagagtgatg acagtgatga aaaaagctgt gtgctttcag caggatttgc atcaggcatc
     1741 agtgcacgat cgccggatgc ggcgtgaacg ccttatccag cctaccaaac ccgcactgat
     1801 tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc tgcgcacaaa tgccttatcc
     1861 gcctaaaaac attaaacccg caataacgcg cttccgagat aatcgttcgc gtccttcact
     1921 cccggcagcg aaaaaaaata accgccgccg ataggtttaa cgtattcctc cagcgcttcg
     1981 ccattgagcc ttttttgtac tgtcaggaag cctttttcca gatcgtgttg gtagcagaca
     2041 aacagcaacc ccatatccag ttgtccggag ttggtgaccc ccaacgaata actgtagccg
     2101 cgacgcagca tcaggctgga ctcactctcc gccgtgcggg gattcgccag ccggatatgg
     2161 ctgtccagcg caatcacctt cccttccggg tcgctggcgt aatcaggcac atcatgctca
     2221 tgctgcattc ccagcggcgc accggtttgc ttatcacggc caaaaatcgt ctgctgttct
     2281 ttcagcggcg ttctgtccca aaattccact cgaaactgaa tcaagcgtac tgcctgatag
     2341 ctgccaccga ttgtccacgc aggctcctgc tgatctgccg ttacccacac cactttttgc
     2401 atcaacttat cattctggct atcgggattg gcagtgccgt ctttgaaacc cagcaaatta
     2461 atcggcgtct ctttgccttt actacgcgac acgtgatcgg aaataaaccc ttcccgcttc
     2521 cagcgcacac tgagcaaatc cggcgtgtgt ttgatgatat cgcgcagcgc atggataacc
     2581 gtgtcctggg tgttggcgca aatctgtagc aacacatcac catgacataa cgccgcatcc
     2641 agcgagtcgt tggggaaacg cgtcatcttc tgcagctttt ttggcatctg tggcgcaagg
     2701 ccaaagcgct catcaaacaa tgagtgaccc accgataacg tgatggtgag attatcgggc
     2761 gcaatgtagc cgccaagaat gccggaatcg agtggtggca ggcgcggatt tggcgtttct
     2821 ggtgctgctc cgccctgagt cagaaaagca aaacgctgag tcaacaagcg aaacaaccgc
     2881 tcaagatcgg ctttatcgct ggcaagcaca tcaaacgcca ccagcatcat tgcggcctgt
     2941 tgtggcgtca ggatccctgc ctgatgctca ccataaaacg gctgtttctc attgcgtgca
     3001 tccggtgaaa gcgtgcccgg tgcactttgc gttttttgtg catgagcgac cggacaactt
     3061 cccgccagcg ccagcgcacc aatccctttc agtaaacgtc ggcgtgacgg ttcattcacg
     3121 ccgttttcat ctttatactg cataacgctt aatccagtcc cagcacaccg cgaagttgcg
     3181 ccagatcttc cgccagcgca gtaatcggtc ctttcagtgc attccggtcg gcatcggtca
     3241 atttgtcgta ggtttcaaaa ccgtctttag tacggtattt cgccagaatg gtatcgacct
     3301 ttttaaagtt ggcatcgact tttgccagca gttccgggtt ggctttttgc agttgtggac
     3361 gcagcaaatc gacaattttc tgcgagcctt caacgttagc ctggaaatcc cacagatcgg
     3421 tgtggctgta gcgatcttct tcaccgctaa ttttgctggc tgccacttcc tcaatcagtc
     3481 cggctgcgcc gccgaccact tttgaaggtg ggaaagccag ttcactgatg cgtttttgca
     3541 aatcgaccac atctgtataa agctgctcag cgtactgatc catccctttg gtggtgttgt
     3601 cgccaaacaa tgctttttcc agacggtgga aaccagtgaa ttttgggtcg gcggcttttt
     3661 gctcgtaatc atcttcacgg gcgtcaatgc tgccatccag atcggagaac agttcagcaa
     3721 tcggttcaat gcgctcatag tgctggcgcg tcggcgcata cagtgctttc gctttttcga
     3781 tatcgcctgc tttaatcgcg tcggtaaagg ctttggtgtc ggtcaccagc tgcgtggttt
     3841 ccgccatgac atacgcttta tatgcagtaa ttgcaccacc aagacttaac agcgcatcac
     3901 tttgcgccgc atccgccgtt gcctcacctt tgacgatcaa cttccctttc gggttagtca
     3961 gcagaccgca ggtcatatcg tattcgccag gctgtaaatt cgccgtcatt ttctggctaa
     4021 aaccaggggc gatattttcc cgctcttcca ccaccatcac gcctttgagg atctcccact
     4081 ccagcgcctt ctggctatgg ttctgaataa tgaactgtgt tttcccggcg ttaaccgtaa
     4141 tggtcatcgg ttcgcactgc ttatccgtca cggtcacttt gacctgcggc acatcagcgg
     4201 cgttagccat aaaagcagaa gaaaacagcg cagccacgct caactgcaat gcgttacggc
     4261 ggaagttaat ggtcatgacg agactatccc tttaaagtat gttgtaacta agcaagagtt
     4321 tgcgtcgtat ttgttacgcg gagcgagacg ctgtcgcccc tgcgcgtggt ggcagagcaa
     4381 atgccaccag cgccgggatg agataaataa accagacggc gacttcgctg acgctcggtg
     4441 cttcctgata gccaaaaatc ccttccatca gcgtgccaaa cagcgagtga gttgagagca
     4501 ctgcgctcat atcgaaggcg atttcctgaa agtggttcca caatccggct tcatgaaatg
     4561 cgcgaatggc accagcagcc agccctgcgg cgacgaagag aataaacagg ctggtccatt
     4621 taaaaaatgc accaagattg aggcgaatac cgccccagta gagcaggaag cctagcacca
     4681 cggcagtagc aagaccgagc attgcaccca gcggcggcca gatcccgaca tcttgttgaa
     4741 atgccgccag caggaaaaag accgactcca gcccttccct tgcaacggca aaaaagacca
     4801 tcatcaccag cgcccagcca tgatgatttc cacgctgcaa tgcgctatcg actgcctgct
     4861 ccagttgtac tttgacgttg cgcgacactt tgcgcatcca gaaaaccatc caggtaagga
     4921 tcaccacggc gatcaccgcc acgatacctt caaacagttc ctgttctttt tgcggaaatt
     4981 cgccggtggt ttcgttaatg aagataccca agcccaggca caacgcagcg gcaagcaaca
     5041 cgccaatcca catcacgcca atccatcggc ctcgctgggt acgcttaaga tagctggcaa
     5101 tcaaactgac aatcagcgcg gcttcaagtc cttcgcgcaa cataatgaga aacggaacaa
     5161 acatgccacc cacccttaaa cgtttttctg agtcaatcca tgcaaagaaa agtaaatcgc
     5221 agtgatagcg attatcatta cggcaaagaa aaatacaagc ggaatgttta taaaatgatg
     5281 ttggaatgta aacaaagaga ggtaatacca ggggatataa acgattattc caggtacaaa
     5341 gatgggattg tttacaacga gccatgtgtt cttgccagat gccgcgtaaa cgccttatcc
     5401 agccgacaaa actgtccaga ctcaatgcat cacagcttcc atgtaaaact gataagcggg
     5461 tgtatcaagc aatttcgtcc atccgcgatt atcaaatcta actttttgcc cggaacataa
     5521 taacagcgcg ggtgttggtg gcgtcaaaat cgctgaaacg ttccccgaag gccggggcag
     5581 cccacatgga tgtgggctga gggcgcgtct tacaggacgt tacctcgcgc ccgacccggt
     5641 agccgaaggg gataa
//
LOCUS       DAZRUF010000239         5640 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZRUF010000239 DAZRUF010000000
VERSION     DAZRUF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.240"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHXOL_003641"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHXOL_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR6810524.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHXOL_003642"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHXOL_003642"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR6810525.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHXOL_003643"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHXOL_003643"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR6810526.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHXOL_003644"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHXOL_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR6810527.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1352 c         1529 g         1358 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAZRUF010000240         5629 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZRUF010000240 DAZRUF010000000
VERSION     DAZRUF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.241"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..353
                     /gene="traA"
                     /locus_tag="ACHXOL_003645"
     CDS             <1..353
                     /gene="traA"
                     /locus_tag="ACHXOL_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR6810528.1"
                     /translation="LSVQGTSAPVKKKSFFSKFTRLNMLRLVRAVIPVAVLMMLFPEL
                     AMAAGKGTDLMAKGQETVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVGFAI
                     LSVFIAVGMSVAGF"
     gene            368..679
                     /gene="traL"
                     /locus_tag="ACHXOL_003646"
     CDS             368..679
                     /gene="traL"
                     /locus_tag="ACHXOL_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR6810529.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            701..883
                     /locus_tag="ACHXOL_003647"
                     /pseudo
     CDS             701..883
                     /locus_tag="ACHXOL_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
     gene            885..2135
                     /locus_tag="ACHXOL_003648"
                     /pseudo
     CDS             885..2135
                     /locus_tag="ACHXOL_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2132..2518
                     /gene="trbI"
                     /locus_tag="ACHXOL_003649"
     CDS             2132..2518
                     /gene="trbI"
                     /locus_tag="ACHXOL_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR6810530.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2515..2826
                     /locus_tag="ACHXOL_003650"
                     /pseudo
     CDS             2515..2826
                     /locus_tag="ACHXOL_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
     gene            2821..3819
                     /locus_tag="ACHXOL_003651"
                     /pseudo
     CDS             2821..3819
                     /locus_tag="ACHXOL_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            3839..4586
                     /gene="traX"
                     /locus_tag="ACHXOL_003652"
                     /pseudo
     CDS             3839..4586
                     /gene="traX"
                     /locus_tag="ACHXOL_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            4641..5201
                     /locus_tag="ACHXOL_003653"
     CDS             4641..5201
                     /locus_tag="ACHXOL_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR6810531.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5331..5543
                     /locus_tag="ACHXOL_003654"
     CDS             5331..5543
                     /locus_tag="ACHXOL_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR6810532.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1310 a         1389 c         1736 g         1194 t
ORIGIN      
        1 cgttaagtgt tcagggtact tctgcgcccg tcaaaaagaa atcgtttttt tctaaattca
       61 ctcgtctgaa tatgcttcgc ctggttcgtg cagtgatccc ggttgctgtt ctgatgatgt
      121 tgttccctga gttagctatg gctgctggta aaggcacgga cctgatggct aaaggtcagg
      181 aaacggttaa ggcgaccttc ggtaaggact ccagtatcgt taaatgggtt gttctggctg
      241 aagttctggt cggtgcagtc atgtacatga tgaccaaaaa cgtcaagttc ctggtcggct
      301 ttgccatact ctctgtattt attgcagtag gtatgtctgt tgccggattc tgacaggaaa
      361 taaaacgatg ttgggagacg agaataaact taagaaatat cgtttcccgg aaacactgac
      421 caaccagagc cgctggtttg gcctgccact ggatgaactg atccccgcag caatctgtgt
      481 tggctggggt atcacaacat cgaaatatct gttcggtatt ggtgcagcgg ttctggttta
      541 tttcgggatt aaaaaactga aaaaagggcg gggcagttcc tggttacgtg acctgattta
      601 ctggtatatg ccaacagccc tgctgcgcgg tatttttcat aatgttcccg attcgtgttt
      661 ccggcagtgg attaaataga actgatacca ggattgttat atggaacacg gtgcccgttt
      721 aagtaccagt cgtgtaatgg ccatcgcctt tatatttatg tcagtgctta ttgttctcag
      781 cctctctgtt aacctcattc agggggtgaa taactaccgt cttcagaatg agcaacgcac
      841 tgccgtgacc ccaatgggat ttaatgtgcc ctttgccgtg tcacacctac cgtaaccagc
      901 tggcgtttat cgatattttc ttccggggaa tgaataacac caactacaac atggcggtct
      961 gtggcacctc cggggccggt aaaaccgggc tgatacagcc acttatccgc agcgtgctgg
     1021 actccggggg ctttgccgtg gtgttcgaca tgggggatgg ctacaagtcc ctgtgtgaga
     1081 acatgggggg cgtatatctg gacggtgaaa ccctgcgttt taacccgttt gcgaacatca
     1141 ccgatatcga ccagtcagcg gagcgtgtcc gtgaccagtt gtcagtgatg gccagcccca
     1201 acggtaacct ggatgaagtg catgaaggtc tgctgctgca ggcggtcagg gcctcctggc
     1261 tggccaaaga gaacagggca cgtattgatg acgtggtgga tttcctgaaa aacgccagtg
     1321 acagcgagca gtatgccggg tcaccgacta tccgcagccg tctggacgaa atgattgtgc
     1381 tgcttgacca gtacactgcc aacggcactt acggccagta ttttaactct gatgagccgt
     1441 ccctgcggga tgacgcaaaa atggtggtgc tggagctggg cggactggaa gaccgtccgt
     1501 cactgctggt tgcggtgatg ttctccctga ttatctacat cgagaacagg atgtaccgca
     1561 cgccgcgtaa cctcaagaaa ctgaacgtca ttgatgaagg ctggcgtctg ctggacttca
     1621 aaaatcacaa ggtcggtgag tttattgaga aaggttaccg taccgcccgt cgtcataccg
     1681 gtgcttatat caccatcaca cagaacatcg tcgactttga ctctgacaag gcctccagtg
     1741 cggcccgtgc ggcatggggt aactcctcct acaaaattat cctcaaacag agtgcgaagg
     1801 agttcgcaaa atacaaccag ctgtatccgg accagttcca gccactgcag cgcgacatga
     1861 ttggtaagtt tggtgcggcc aaagaccagt ggttcagttc cttcctgctg caggtggaaa
     1921 accattcctc ctggcaccgc ctgtttgtgg acccgttaag ccgcgccatg tacagctctg
     1981 acggcccgga ttttgagttt gtgcagcaga aacgtaaaga ggggctgagt attcatgagg
     2041 cggtgtggca gctggcgtgg aagaagtcag ggccggagat ggcttcgctg gaagcctggc
     2101 tggaagaaca tgagaaatac aggagtgttg catgaccaca acgcaaaaaa cgactgatgt
     2161 tacagccccc cgacgcagcc actggtggtg gacggtgccg ggatgcctgg caatggtgtt
     2221 actgaacgca gccgtcagtt acggcattgt cagactgaac gcaccgatga ctgccgcctt
     2281 caacatgaaa cagactgtgg atgcattttt tgacagcgcc agtcagaaac aactgtcaga
     2341 agcacagtca aaagcccttt cagcgcgttt taacacggca ctggaagcca gcctgcaggc
     2401 gtggcagcag aaacaccacg cggtcattct ggtgtcgcct gccgtggtac agggggctcc
     2461 ggatatcacc cgggaaatcc agcaggatat tgcccggcga atgagagcgg aaccatgaga
     2521 tgccgggggc tgattgccct gctgatatgg gggcagagtg tggccgccgc cgatcttggc
     2581 acctggggtg atctgtggcc ggtaaaggag ccggacatgc tgacggtgat catgcagcgc
     2641 ctgacggcac tggagcagtc cggtgagatg ggccggaaaa tggatgcctt taaggagcgg
     2701 gtgatccgca acagtctgcg gccccctgcc gtgcccggta tcggacgcac ggagaaatac
     2761 agcagccggt tgtttgaccc gtccgtcaga ctggctgcgg atatccggga taacgaaggg
     2821 cgggtggccg gctttgagtc agcctacgtg gccctgtcgc gtatgaagca gcatgtgcag
     2881 gtgtacaccg ataaccgtca gggctggacg gatgccatca acaatgccgt acagaaagga
     2941 actgcccacg atgtgtttga gccgaaaccg gaccgggagg tcatgaatgc agagcggctg
     3001 ttcagtacgg cgcgggagct gcgggacgtg gcggcaggcc gtgccgttct ccgtcaggcg
     3061 gggctggccg ggggagacag tcctgcacgg tttattgctc cgggacgtaa atatccgcaa
     3121 ccgtatgtgg cactgccggc gtttgaccgt aacggcaagt ccgccggtat ctggctgaac
     3181 ccgctgacca cggatgacgg aaacgggctg cggggattca gcggtgaagg tcgggtaaaa
     3241 ggcagcgggg atgcgcagtt cgtggccctg cagggcagcc gtaacggaga aagcctgctg
     3301 gctgataata tgcaggatgg cgtccggatt gcccgtgata atcctgacag tggtgtggtg
     3361 gtaagcattg ccggtgaagg ccgtccgtgg aatcccggtg ccatcaccga tggccgcgtg
     3421 tggggggata tcccggacaa cagcgtccag ccgggagccg gaaatggcga accggtcacg
     3481 gcagaggtac tggcacagcg gcaggctgaa gaggccatcc gccgtgaaac ggaacgtcgc
     3541 gcagatgaaa ttgtccgtaa aatggcagag aacaaacctg acctgccgga cggcaaaaca
     3601 gagctggctg tcagggaaat tgccgggcag gagcgtgacc gggctgccat aactgaacgg
     3661 gaagccgcgc tgccggagag tgtgctgcgt gaatcacaac gggagcggga agcggtccgg
     3721 gaggttgccc gggaaaatct gctgcaggag cgtctgcagc agatggagcg ggatatggtt
     3781 cgtgacctgc agaaagagaa aaccccgggc ggagactgat acaggaagac aaacactgat
     3841 gacaacggat aacacgaaca cgacacgtaa cgattcactg gctgcacgga ccgatacctg
     3901 gttgcagtca tttctggtct ggtcacccgg acagcgggat atcatcaaaa cggtggcact
     3961 ggtgctgatg gtactggacc acatcaatct gatattccag ctgaagcagg aatggatgtt
     4021 tctggccggg cgaggagcct ttccgctttt tgccctggtg tgggggctga atctgtcccg
     4081 tcatgcgcat atccggcaac cggccattaa ccggctgtgg ggatggggaa ttattgccca
     4141 gttcgcatat tatctggcag gctttccctg gtatgagggg aatatcctgt ttgcctttgc
     4201 agtggcggca caggtgctga cgtggtgtga aacgcgcagt gggtggcgta cagcagcggc
     4261 cattcttctg atggccctgt tgggggcctt tgtccggcac cagttacggc attgccgggc
     4321 tgctgatgct ggcagtcagc caccgactgt accgggcgga agacagagcg gaacgtctgg
     4381 cgctggtcgc ctgcctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag
     4441 cggcggtggc cggtcttgtg atgacggtgc tgaccgttgg tctggtgtcg tgtgcaggaa
     4501 aatcattacc ccgtttctgg cccggggatt ttttcccggt attctacgcc tgtcatctgg
     4561 ctgtgctggg cgttctggcg ctgtgacggg tgtggtatct ttgaccgtaa caggatgatt
     4621 cgtcagaggc agaacacagc atgacagagc agaagcgacc ggtactgaca ctgaagcgga
     4681 aaacggaagg ggagacgcct gtccgcagcc ggaaaaccat catcaatgtc accacgccac
     4741 caaagtggaa ggtgaaaaag cagaagctgg ccgagaaagc agcccgggaa gcagagctgg
     4801 cggcaaaaaa agcgcaggcc agacaggcac tgtccattta tctgaacctg cccacgctgg
     4861 atgaggccgt gaataccctg aaaccctggt ggccgggatt atttgacggt gacacccccc
     4921 ggcttctggc ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag cggaatatcc
     4981 cgctctcgca taaaaaactg cgcagggcgc tgaaggccat cacccgttca gaaagctatc
     5041 tgtgtgccat gaaagccggt gcctgccggt atgacacgga agggtatgtg acggagcata
     5101 tttctcagga ggaggaagcg tatgcggcag cgcgtctgga taaaatccgc cgccagaacc
     5161 ggataaaggc agaacttcag gccgtgcttg atgagaaata aaaaaaagcc tccatcagag
     5221 aggaggcagg gaaataaggc ttaaaaggaa ataacttccg cttccgaaag gaagccagtc
     5281 cactgaatta tttatcacaa taatactgac aactaaaggg ggacaagaaa atgaatggat
     5341 tcagaaacag ttcacggaac ggccaggtct ggcgttacca gcgtgccggc gggcgggccg
     5401 ttattctgga agtcagtgga cgctggatgg aagcggcaga agcatggcga cgggctgcct
     5461 gtgttgcgcc caggacagac tggcagcagt ttgcccgaaa aagggctgaa cactgccacc
     5521 ggcgctgccg gggaagggtg taacgaaaaa agcagccttc attactccag gagtcagaag
     5581 gctgaaagga gtttatgaga tttgcttttt ttaatatccg gtcgggcag
//
LOCUS       DAZRUF010000241         5626 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZRUF010000241 DAZRUF010000000
VERSION     DAZRUF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.242"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            76..678
                     /locus_tag="ACHXOL_003655"
     CDS             76..678
                     /locus_tag="ACHXOL_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR6810533.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(680..1201)
                     /gene="ruvC"
                     /locus_tag="ACHXOL_003656"
     CDS             complement(680..1201)
                     /gene="ruvC"
                     /locus_tag="ACHXOL_003656"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR6810534.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1236..1976)
                     /locus_tag="ACHXOL_003657"
     CDS             complement(1236..1976)
                     /locus_tag="ACHXOL_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR6810535.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(2005..2457)
                     /gene="nudB"
                     /locus_tag="ACHXOL_003658"
     CDS             complement(2005..2457)
                     /gene="nudB"
                     /locus_tag="ACHXOL_003658"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR6810536.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2575..4347)
                     /gene="aspS"
                     /locus_tag="ACHXOL_003659"
     CDS             complement(2575..4347)
                     /gene="aspS"
                     /locus_tag="ACHXOL_003659"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR6810537.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            4657..5223
                     /locus_tag="ACHXOL_003660"
     CDS             4657..5223
                     /locus_tag="ACHXOL_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR6810538.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5220..5315
                     /locus_tag="ACHXOL_003661"
                     /pseudo
     CDS             5220..5315
                     /locus_tag="ACHXOL_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1352 a         1507 c         1344 g         1423 t
ORIGIN      
        1 attgcgcctc ctgaaaaaaa aataaatttt ttatgctatt acgtatattc gtatctattt
       61 caatggaatg acaacgtgaa tattaattac cctgctgaat atgaaattgg agatatcgtc
      121 tttacatgta taggtgctgc cttatttggt caaatatcag ctgcatcaaa ttgctggagt
      181 aatcacgtcg ggatcattat cggtcataac ggtgaagact tcctggttgc agaaagccgg
      241 gttccccttt caaccatcac cacgctatct cgttttatta aacgttcgtc taatcaacgc
      301 tatgctataa agcgattaga cgctgggctg acagaacaac aaaaacaacg aattgttgaa
      361 caggttccct cccggctacg caaactttac cacaccggtt ttaaatacga atcttcgcgc
      421 cagttctgtt caaaatttgt ttttgatatt tataaagaag cgctatgcat tccggtgggt
      481 gaaatagaaa cgtttggaga actgttaaat agcaatccga atgcaaaact cactttctgg
      541 aaattctggt tcttaggttc tattccgtgg gagcgtaaaa ccgtcacgcc agccagtttg
      601 tggcatcatc cgggtctggt gttgattcac gcggagggag ttgaaacgcc tcagcctgaa
      661 ctgaccgagg cggtataact taacgcagtc gccctctcgc caggttcagc cgcgattcgc
      721 tcatctgcat tgcattctgg ctgacgtgac aatgggtaat cgcgatagct aacgcatcgg
      781 cggcatccgc ctgtggatta gcgggcagtt tcagcaaggt gcggaccata tgctgcacct
      841 ggcttttttc ggcactacca atacctacca ccgtttgctt tacctgacgt gccgcgtatt
      901 caaataccgg caattcctga ttcaccgccg ccacaatcgc cacgccgcgc gcctgcccca
      961 gtttcagagc cgagtcggca tttttggcca taaagacttg ttcaatggcg aaataatctg
     1021 gctggaactg ggtgatgatt tccgtcacgc ccgcatagat gagcttcaga cgagacggta
     1081 aatcatccac tttggtgcgt atgcatccgc tacccaggta ggacagttgc ctgcctacct
     1141 ggcggatgac accatagccg atcacgcgcg aacccgggtc aatgccgaga ataatagcca
     1201 tcacgcgtct ccgttttgct gtttagcagg ccccatcaga gagttgctgc aacctcatca
     1261 gagatttcac cgttatggta gacttcctgc acgtcgtcgc aatcttccag catatcgatc
     1321 agacgcatca gtttcggtgc ggtttctgca tccatatcag ctttggtgga cgggatcatg
     1381 gaaacttccg cgctgtctgc tttcagacct gccgcttcca gagcgtcgcg cactttaccc
     1441 atttcttccc atgcagtgta gacgtcaatc gcgccgtcat cataggtcac aacgtcttca
     1501 gcacctgctt ccagtgctgc ttccataatg gtgtcttcat cgcctttctc gaaggagatc
     1561 acgccttttt tgctgaacag ataggcaacg gaaccatcag taccgaggtt accgccacat
     1621 ttgctaaatg catgacgcac ttcagcaacg gtacggttgc ggttgtcaga cagacattca
     1681 atcatgattg ccgtgccgcc aggaccgtaa ccttcgtaga tgatggtttc catgtttgca
     1741 tcatcatcac cgcccacacc acgtgcaatt gcgcggttca gtgtgtcacg ggtcatgttg
     1801 ttagacagtg ctttatccac cgccgcacgc agacgcgggt tagcgtccgg atcgccaccg
     1861 cccagcttag ccgcggttac cagctcacga atgattttag tgaagatttt accgcgctta
     1921 gcatcctgcg cagctttacg atgtctggtg ttggcccatt tactatgacc tgccataaaa
     1981 atatctccag atagccctgc ctgttcaggc agcgttaatt acaaactgtt caatcgcctg
     2041 ccggttgctc caggacttag tgagcgccgc ggcagcaggc gcatcaagcc acttgtaagc
     2101 cagatgttca gtgaagacta cctggcgttc gtgcgggagc gcaagacaga accatgattc
     2161 cgtattacgc gtcacgcccg gcgcatagcg atgacgtaaa tgtgaaaaaa tttcaaactc
     2221 taccgtgcgc tgacagtcaa ttaaggtcag ttgttcagcg acaacatcaa tggtgacctc
     2281 ttcctttact tcgcgcatgg cagcttgcgg cgcggtttca ccctcttcca cgctgccggt
     2341 taccgactgc cagaaatcgg gatcgtcacg ccgctgcaac atcagcaccc gtttcgtatc
     2401 ttgtgcatag atgaccacta agatcgaaac gggacgctta tacactttat ccttcacgct
     2461 gcctcttcgt tgactgcctt cgctcatccc attcacatag ttatctatgc tcatgggagt
     2521 tcactcagtt gccgccttga tgcaacgcga atgatttcgt gtatttgagt catatcagtt
     2581 attctcagcc ttcttcacaa cctgaatgct cagctcagcc agtacagtcg ggttagcaaa
     2641 gctcggtgct tcagtcatca gacacgctgc cgccgtggtt ttcgggaagg cgataacgtc
     2701 acggatattg tcggtgccag tcagcagcat ggtcagacgg tcaagaccga atgccagacc
     2761 tgcgtgcggc ggagtaccgt atttcagggc gtcgagcagg aagccgaatt tctcgcgctg
     2821 ttcctcttcg ttgataccca gaataccaaa caccgtctgc tgcatatcac cattatggat
     2881 acgtactgaa ccaccgccca cttcgtaacc attgatgacc atatcgtaag cgttcgccac
     2941 cgcattttcc ggtgcagctt tcagttctgc agcagtcatg tctttcggtg aggtgaacgg
     3001 atggtgcatt gccgtcaggc cgccttcacc gtcgtcttca aacatcggga agtcgataac
     3061 ccacagcggt gcccatttgc tttcgtcggt cagaccaagg tctttaccca ctttcaggcg
     3121 cagtgcgccc atcgcgtcgg caacaatttt cttgttgtcg gcaccgaaga aaatcatatc
     3181 gccatcttgc gcggcagtac gatccaggat ggcttcgatg atttctgcat taaggaactt
     3241 cgctaccggg ctgttgatac cttccagacc tttcgcgcgt tcgttaactt tgatgtaagc
     3301 cagacctttc gcgccgtaga ttttaacgaa gttaccgtat tcgtcgatct gcttacgggt
     3361 cagcgatgcg ccacccggaa cacgtagagc ggcaacacgg cctttcggat cgttcgccgg
     3421 ccccgcaaat actgcaaact caacagattt cagcagatcg gcaacgtcgg tcagttccat
     3481 cgggttacgc agatccggtt tatcagaacc ataacggcgt tctgcttccg caaaggtcat
     3541 taccgggaaa tcgcccagat ccacgccctt cacttccagc cacaaatgac gcaccagcgc
     3601 ttccatcact tcacgcactt gcggcgcggt catgaaagaa gtttccacat cgatctgagt
     3661 aaattcaggc tgacgatcag cgcgcaggtc ttcgtcacgg aagcatttaa cgatctgata
     3721 gtaacggtca aaaccggaca tcatcagcag ctgtttgaac aactgtgggg attgcggcag
     3781 tgcgtagaat ttacctttgt gcacacgaga aggcaccaag tagtcacgcg cgccttccgg
     3841 cgtggctttg gtcagcatcg gagtttcgat gtcgaggaag ccgtggtcat ccataaaacg
     3901 gcgcaccagg ctggtgattt tagcgcgggt tttcaggcgc tgcgccattt ccgggcgacg
     3961 caggtcgagg tagcggtatt tcagacgcgc ttcttcggtg ttgacgtggt tagagtcaag
     4021 cggcagaaca tctgcgcggt tgatgatagt cagcgaggac gccagcactt caatttcgcc
     4081 ggtcgccata tcgcggttaa tatttttttc gtcacgcgca cgtacggtgc ccgtaacctg
     4141 aatgcagaac tcattacgca gttcagaggc cagctttaac gcgtccgcac gatccggatc
     4201 gaaaaatacc tgcacgatac cttcgcggtc gcgcatatcg atgaagatca ggctaccaag
     4261 atcacgacga cggttgaccc aaccacacag agtcacctgc tgccccacgt gggacaaacg
     4321 gagctgtcca caatattctg tacgcatgag atatccctta acttagctgc cggcggatgc
     4381 cccctgctgc gcaggtgacc aagtcgcagc gttagctgta tgtcacaact gaatgaaaaa
     4441 aggcggctat tatactggaa attctgccgc accgtaagag cctggcccgc gctgtaacgc
     4501 ctcgttacca ctttatatcg ggcctgaaat cagactctac gccagtttgc tataaaggtg
     4561 ttgcccgaac tcataaaaat taacaaaatt tgtcgttccg ccatcggcta atcgcattaa
     4621 ggtgtgaggc acgattttgt tttttcagga gtcagcatgc ttgaacttaa tgctaaaacc
     4681 actgcgttgg tggtgatcga tttacaagaa ggcatcttac cttttgccgg tggtccacat
     4741 actgccgatg aggtagttaa tcgcgccggg aagctggcgg cgaaatttcg cgccagcggt
     4801 cagcccgtgt ttctggtgcg agttggctgg tctgccgatt acgccgaagc attaaaacag
     4861 ccggttgatg ccccctcccc cgctaaagtg ttgcccgaaa actggtggca acatccagct
     4921 gcattaggtg caactgacag tgatatcgaa atcatcaaac gtcagtgggg tgcgttttac
     4981 ggtacggatc tggagttgca actgcgccgc cgaggtatcg atacaatagt gttatgtggg
     5041 atctcgacca atatcggtgt tgaatccacc gcccgcaatg cctgggaact tggctttaat
     5101 ctggtgattg ccgaagacgc ctgtagtgcc gccagcgccg agcaacacaa taacagcatt
     5161 aatcatatct acccgcgcat cgcccgtgtg cgtagcgtgg aagagatcct tcacgcgtta
     5221 tgatttacat cggtctaccg caatggtcgc atcctaaatg ggtgcggttg gggatcacca
     5281 gccttgaaga gtatgcccgc cactttaact gcgtgatacg ggatttttaa aaatcactaa
     5341 agaacgccca agagcatgtg ttttctttag aataatcaaa gcgttggatt gccatgccat
     5401 atgaaaatcg gcaaagttca tatgtaacaa agtgtcccaa tcatgcccca aaatgcccca
     5461 caacaaatat ttttgcccca tgcatgcccc aaaaaatcac tttccctcac tcttgacact
     5521 gtttatccat acagttaaaa ataatactgt ataaaaacac agtgtagagg gatttttatg
     5581 cgtattgaaa tctgcatagc caaagagaaa atgactaaaa tgccaa
//
LOCUS       DAZRUF010000242         5616 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZRUF010000242 DAZRUF010000000
VERSION     DAZRUF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.243"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..512)
                     /locus_tag="ACHXOL_003662"
     CDS             complement(<1..512)
                     /locus_tag="ACHXOL_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR6810539.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(758..1210)
                     /locus_tag="ACHXOL_003663"
     CDS             complement(758..1210)
                     /locus_tag="ACHXOL_003663"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR6810540.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1207..2262)
                     /gene="dinB"
                     /locus_tag="ACHXOL_003664"
     CDS             complement(1207..2262)
                     /gene="dinB"
                     /locus_tag="ACHXOL_003664"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR6810541.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2333..3118)
                     /gene="lafU"
                     /locus_tag="ACHXOL_003665"
     CDS             complement(2333..3118)
                     /gene="lafU"
                     /locus_tag="ACHXOL_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR6810542.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3090..4802
                     /locus_tag="ACHXOL_003666"
                     /pseudo
     CDS             3090..4802
                     /locus_tag="ACHXOL_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4925..5422)
                     /gene="rayT"
                     /locus_tag="ACHXOL_003667"
     CDS             complement(4925..5422)
                     /gene="rayT"
                     /locus_tag="ACHXOL_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR6810543.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5493..>5616)
                     /locus_tag="ACHXOL_003668"
     CDS             complement(5493..>5616)
                     /locus_tag="ACHXOL_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR6810544.1"
                     /translation="IAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1466 c         1459 g         1352 t
ORIGIN      
        1 gagccgctat gaaccaacag ttgcagatgt tgtgcatcca gacctgccag tgcaaacagt
       61 tcagcgtcga tgatttgatc aacttgttgc agttcagcga aatgattgcc gccaccgata
      121 gagccaagcg agctgcgcca cggatgctgt gcaaatgctg acggcaggtt ttcctccagc
      181 cagctttctt cagcaacgtc atccaaagct gataatcgct tttcaaactt atcggcgttg
      241 tatttgcgag cgagaatatc tgtttgccat agcgccatgc cgcagccgat atcattgccg
      301 accagtgctg ggtaaaaacg gccaacagag aagaacgctg cgccaatcgg gtagccgcgt
      361 ccgggatgta aatctggcat ccccactacg cgctgcatgt tgggtaaatt tgccgtggtg
      421 tgtaattgtt ggatcgctaa actctcgatc cacaggtcat cagatgcgat ggtaaatacc
      481 gcatcggata agggacgaat atatttgccc atgtaccatt ccgtaaatag actcgtcata
      541 cttcaagttg catgtgctgc gactcgaatt attgagagta acaattaaaa aatcaggaaa
      601 tggcagacga atccagggca agaaaaaaag aattaatcct ggaaaggtct gcaaagagtc
      661 attgataaag gatttgtcat gatttacctc cttgcagttt gtggaataag aaaacgcgca
      721 catactaggc gggcgttatt ttcattgcaa gctggattta atgttgcggt ttatattgca
      781 tataaaaatt agtaaaccac gctccccggc attcaacgtg ctgctgctta actgtctgaa
      841 aaccataacg ttcaaaaaag ggttttgcgg ttatgctggc atctaccgta agttcggatt
      901 cagacttaat aaatggtttt aacaaggcgc tggcaacccc gcggcgggtg tattcagggt
      961 caacaaataa catatcgata taatgttcaa tgcgggaaat aaaaccaacc ggttgtgcat
     1021 taatgaccgc aacccacact tgtgatttcg cgagtttctc cttccagcga gattcgtcaa
     1081 tctgcgccca ggcggcaatt tgttgtggag aataatgctg actggcggtc atcgtaaccg
     1141 ctctaatgaa aatagcgcat agttgctgaa aatcgccagg ctgatagttt cttatttgta
     1201 tgttattcat aatcccagca ccagttgtct ttccatttgc ggatcaagca acgtcacatg
     1261 cagccccacc agtcgcacac cgcgcccgcc gcggcgttca tcccaggttt tacgcgcggt
     1321 ggcgattaag tcagctttat tcagccgcgg ccagacgtgc tcctgagtgg tttgctgaaa
     1381 atcgtcgaac tttaatttca ccccctggcg ggcaatcagt aaatcaggtt ttacctttgc
     1441 cagacggcgt tcaagttccg gatacagccg ctcgataatc gcttcacatt cagaccagtg
     1501 gtggatatct tccgccatcg tgcgttccac gccgacggat tttcgcaacc gttcgctgtt
     1561 aacgtcgcgc tcgtcaatcc cctgactacg ctcccacaaa atgcggccaa atttgccaaa
     1621 gcgtttaagc agcatcacca gatcacactt ttgtacatca ccgcaggttc gtagccccat
     1681 cgcttccagt tttgccgccg agactttgcc gacgccgggg atttttgcca gtggtaaggt
     1741 ttgtaaaaat gccggaactt ctgccggcgt aatcacaaac tggccgttgg gtttattcat
     1801 gtcggaggcg attttggcga gaaactttac gggtgccacg cccgcagacg ccgtcagttg
     1861 cagctcgttg aaaatcgtct ggcggatttc ctgggcgatg agggtcgcag aaccgtggca
     1921 atggacgcta tcggtgacgt cgagataagc ctcatccagt gacaacggtt caatacgcga
     1981 ggtgtagcgc gagaagattt cgcggatatg atttgaggct tctttgtagg cgtcaaagcg
     2041 ccccggaagc aaggtgagat gcgggcataa tttgagcgcc atccctgtcg gcatagcgct
     2101 acgtacgcca aatttacgcg cgggataatt ggcggtactg atcacccccc gacgttcgcg
     2161 gctgccgcca atagcaatag ggatatcgcg cagggcggga ttgtcgcgca tctccaccgc
     2221 tgcgaaaaag cagtccatat ccacatgaat gattttacgc attgctcacc tctcaacact
     2281 ggtaaagtat acagtgattt cagagtttga gaaatgcgta aagattcagc atttaacgcg
     2341 ttcgctgcga aagcacctgc tgcttatcca gcttttgcac cagcggctgc accactttat
     2401 ccccatgctg accaaagtat tgatacagcg tatcggacgc acttttggtc agcaccataa
     2461 tctcaatgcg ccggttgccc gcgctttgcg gatttttgga atccagcagc atctggtccg
     2521 ccattgcgct tacctgcatc actttatctt ccggcattcc ggcctcttcc agcacccgac
     2581 gagccgaaag cgcgcgatcg cccgaaaggt tccagttgtt gtagatattg tttttgtaag
     2641 ccatcgcatc ggtatgcccg gtaataataa ttttgttatc gagcgagtcg aaaactggcg
     2701 cgagctccac cagcagcgtt ttaaagaacg gcataatctg cgcgctgccg cgttcgaaca
     2761 tattgcgatt ctggtcgtct ttaatcagca cgcgtaatcc ctggggaaca atctccatct
     2821 ccagattcgc ttccatatgc gcatcacggg caatggtggt gatactggtt gccagctcgc
     2881 caagctcggt ggcggatttc ttcttcagca gcgccgtttt ctcattgacg tcgcgcgctt
     2941 ttttctccgt ttcttccggc accgccacgg tggcaggttt tggcggatga gacgggctga
     3001 ttttatttaa cggcgacagc ccgccaccgt taaaaatcga ctgcccgtgc agcgcggcga
     3061 taatgctttc acgctcggat ttactgacgc tgttgacaat caactttatt gtcgtcacca
     3121 aaggggccga gcgtatctcc gaggtttctg cccgctttac cctggacgcg atgcccggta
     3181 agcagatggc gattgacgcc gatcttaatg ccgggttgat caatcaggcg caggcacaaa
     3241 cccgacgtaa agatgttgcc agcgaggccg atttctacgg cgcgatggac ggggcatcga
     3301 aatttgtgcg cggggatgct atcgccggga tgatgattct ggcgatcaac ctgatcggcg
     3361 gcgtctgtat cgggatcttc aaatacaacc tgagcgccga cgccgccttc cagcagtatg
     3421 tgctgatgac catcggcgac gggctggtgg cgcaaatccc ttccctgcta ctctccaccg
     3481 cggcggcgat tatcgtcacc cgcgtcagcg acaacggcga tatcgcccat gacgtgcgta
     3541 accagttgct ggcaagccca tcggtgctct acaccgctac cggaattatg ttcgtactgg
     3601 cggtagtgcc gggaatgcca catctgccgt ttttgctgtt cagcgccctg cttggcttta
     3661 ccggctggcg gatgagcaaa cgcccgcagg cggcggaagc ggaagagaaa agtctcgaaa
     3721 cgctgacccg caccatcacc gaaaccagcg agcaacaggt cagttgggaa accattccgc
     3781 tgatcgagcc catcagctta agcctcggct acaagctggt ggcgctggtg gacaaagccc
     3841 agggcaaccc gctcacccag cgtattcgcg gcgtgcgaca ggtgatatcc gacggcaacg
     3901 gcgtgctgct gccggagatc cgcattcggg aaaacttccg ccttaagccc agccagtacg
     3961 ctattttcat caacggcatt aaggctgatg aagcggatat tccggcggat aaactgatgg
     4021 cgctgccctc cagcgaaacc tacggcgaga tagacggcgt gctggggaac gacccggcgt
     4081 acgggatgcc ggtgacctgg atccagcctg cgcagaaagc gaaggcgctg aatatggggt
     4141 atcaggtgat cgacagcgcc agcgtgattg ctacccatgt aaataagatt gtgcgcagct
     4201 atattcctga tttatttaac tatgacgata ttacgcagtt gcataaccgt ttgtcgtcga
     4261 tggcaccgcg cctggcggag gatttaagcg cggtgctcaa ttacagccag ttgctgaaag
     4321 tgtaccgggc gctgctgacc gaaggcgttt ccctgcgcga tatcgtcacc atcgccaccg
     4381 tgctggtcgc cagtagcgcg gtgaccaaag atcatattct gctggcagcc gatgtgcgcc
     4441 tggcgctgcg gcgcagcatt acccatccgt tcgttcgcaa gcaggagctg acggtgtata
     4501 ccctgaataa tgagctggaa aatctgctga ctaacctagt gaatcaggcg caacagggcg
     4561 gtaaagtgat gctcgacagc gtgccagtgg acccgaatat gctcaaccag ttccagagca
     4621 cgatgccaca ggtgaaagag cagatgaaag cggcggggaa agacccggtg ctgctggtgc
     4681 caccgcagct gcgccctttg ctggcgcgtt atgcaaggtt gtttgcgccg gggctgcatg
     4741 tgctgtcgta taacgaagtg ccggacgagc tggagttgaa gattatgggg gcgttgatgt
     4801 aagtatccgg cattggtgca caactgccgg atgcggcgta aacgccttat ccggcctacc
     4861 gttcaggcac aagtttgtag gcatgataag acgcgccagc gtcgcatcag gcatctgcgc
     4921 ataattataa aatgatacgc tcgccagcat ttatatccgt tacgtccccc gcccaatcga
     4981 tgggatataa ccctcgcgcg acatcgcgat ggaacgttga gaatggccaa tcactcactt
     5041 gctttaccca accatgcttt actggattta tataaatata atcaacatga tgcctataat
     5101 ctttggtatt gcggatggcg tgctcccaaa aacgtggttg ccagatattt ttcaatccac
     5161 aagcatgggt aaattgcttt ttaatttccc gccagcgaga ggaaaaatca tcatcgcctt
     5221 caggtaatgt ccatatgcaa tgcatatgct ctggcaaaac gacccaggcg ttgatttcaa
     5281 aaggcctgtc tcgcttaact ttaataatgg cgttacggag catctggtac tgggtggtca
     5341 agagttggct tcgacgattg cgtaaattca ccgtgaaaaa ccaggttccc cccttgatgt
     5401 aataacgacg atattcagac atggcgactc ctttttctca acggtaagaa gaaaaggaaa
     5461 ggtcggcaat gaggtgatgg aacagggcaa gattacgaaa gcccgctccc ctcaaggact
     5521 gacgccagat agtttctgtc catggctgct tttcgcatct tacgtcttaa ccctgccttg
     5581 aataccttat cattcgtcaa aatattaata gcgatg
//
LOCUS       DAZRUF010000243         5556 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZRUF010000243 DAZRUF010000000
VERSION     DAZRUF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.244"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..691
                     /gene="yneK"
                     /locus_tag="ACHXOL_003669"
     CDS             <1..691
                     /gene="yneK"
                     /locus_tag="ACHXOL_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR6810545.1"
                     /translation="TGVERVLFLFLNDFIEQFPMFNPGVPIKIAHTPHIEPLPPDHHT
                     AADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKDPNLITAILDITDSELKE
                     DLLQSLMDSLGSNKHVLPEVCICFLFLLAEQESPHFQDLFLFFANMLLHYHQFMNPNE
                     SDLNDVLMPASLSDDKIIKHMARRTLELFVKNETPPKVTHEDLVKNRPRSPVRPPIPA
                     TSKTPDLPERH"
     gene            841..2031
                     /gene="ydeA"
                     /locus_tag="ACHXOL_003670"
     CDS             841..2031
                     /gene="ydeA"
                     /locus_tag="ACHXOL_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR6810546.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2056..2721)
                     /gene="marC"
                     /locus_tag="ACHXOL_003671"
     CDS             complement(2056..2721)
                     /gene="marC"
                     /locus_tag="ACHXOL_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR6810547.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2933..3367
                     /gene="marR"
                     /locus_tag="ACHXOL_003672"
     CDS             2933..3367
                     /gene="marR"
                     /locus_tag="ACHXOL_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR6810548.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3387..3770
                     /gene="marA"
                     /locus_tag="ACHXOL_003673"
     CDS             3387..3770
                     /gene="marA"
                     /locus_tag="ACHXOL_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR6810549.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3802..4020
                     /gene="marB"
                     /locus_tag="ACHXOL_003674"
     CDS             3802..4020
                     /gene="marB"
                     /locus_tag="ACHXOL_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR6810550.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4077..5516)
                     /locus_tag="ACHXOL_003675"
     CDS             complement(4077..5516)
                     /locus_tag="ACHXOL_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR6810551.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         1449 a         1378 c         1233 g         1496 t
ORIGIN      
        1 gaccggcgtc gagcgtgtgc tctttctttt tctgaatgat tttattgaac aatttccaat
       61 gttcaaccct ggcgttccga tcaaaatagc gcatacacca catattgaac ccctgcctcc
      121 ggatcaccat accgctgcag attacttacg ccagtttgat ttgcttgtcc tgaattttat
      181 ctctcgcggt aattttgtca tactcccccg attatggaat aactctgagg ttcacagatg
      241 gttcgtcaat aaagatccta acttgatcac cgccattctc gacataacgg acagcgaatt
      301 aaaagaggat ttattgcaaa gcctgatgga ttcattaggt tctaacaaac atgtactacc
      361 cgaagtctgc atctgctttt tattcctttt agcagaacaa gaatctcctc attttcagga
      421 cttgttttta tttttcgcca atatgttatt gcactatcac caatttatga atcccaatga
      481 aagtgatttg aatgacgtgt tgatgccagc atcattaagt gatgataaaa ttatcaagca
      541 tatggcgcgc aggaccctcg aactgtttgt aaaaaatgaa acaccgccaa aagttactca
      601 cgaagacctg gtgaaaaaca ggcctcgctc ccctgtcaga ccacctatac ccgcaacctc
      661 caaaacgcca gacctccctg aacgtcatta aaccgtgatg ttaccgactc tctgacgcgg
      721 aacagaatca gcgtcagaga aacggaaaac gcgatccaga tcacaaatac attgtattca
      781 catcattaac cgttttaaga tcatttcatc actttttcgc aactcacccg ataatctgtt
      841 atgacaacaa acactgtttc ccgcaaagtg gcgtggctac gggtcgttac gctggcagtc
      901 gccgccttca tcttcaacac caccgaattt gtccctgttg gcctgctctc tgacattgcg
      961 caaagttttc acatgcaaac cgctcaggtc ggcatcatgt tgaccattta cgcatgggta
     1021 gtagcgctaa tgtcattgcc ttttatgtta atgaccagtc aggttgaacg gcgcaaatta
     1081 ctgatctgcc tgtttgtggt gtttattgcc agccacgtac tgtcgttttt atcgtggagc
     1141 tttaccgttc tggtgatcag tcgcattggt gtggcttttg cacatgcgat tttctggtcg
     1201 attacggcgt ctctggcgat ccgtatggct ccggccggga agcgggcaca ggccttgagt
     1261 ttaattgcca ccggtacagc actggcgatg gtcttaggtt tacctctcgg gcgcattgtg
     1321 gggcagtatt tcggttggcg aatgaccttc ttcgcgattg gtattggggc gcttatcacc
     1381 cttttgtgcc tgattaagtt acttccctta ctgcccagtg agcattccgg ttcattgaaa
     1441 agcctcccgc tattgttccg ccgcccggca ttgatgagca tttatttgtt aactgtggtg
     1501 gttgtcaccg cccattacac ggcatacagc tatatcgagc cttttgtgca aaacattgcg
     1561 ggattcagcg ccaactttgc cacggcatta ctgttattac tcggtggtgc gggcattatt
     1621 ggcagcgtga ttttcggtaa actgggtaat cagtatgcgt ctgcgttggt gagtacggcg
     1681 attgcgctgt tgctggtgtg tctggcactg ctgctacctg cggcgaacag tgaaatacac
     1741 ctcggggtgc tgagtatttt ctgggggatc gcgatgatga tcatcgggct tggtatgcag
     1801 gttaaagtgc tggcgctggc accagatgct accgacgtcg cgatggcgct attctccggc
     1861 atatttaata ttggaatcgg ggcgggtgcg ttggtaggta atcaggtgag tttgcactgg
     1921 tcaatgtcga tgattggtta tgtgggcgcg gtgcctgctt ttgccgcgtt aatatggtca
     1981 atcattatat ttcgccgctg gccagtgaca ctcgaagaac agacgcaata gttgaaaggc
     2041 ccattcgggc cttttttaat ggtacgtttt aatgatttcc aggatgccgt taataataaa
     2101 ctgcacaccc atacatacca gcaggaatcc catcagacgg gagatcgctt caatgccacc
     2161 cttgcccacc agccgcataa ttgcgccgga gctgcgtagg cttccccaca aaataaccgc
     2221 caccaggaaa aagatcagcg gcggcgcaac catcagtacc caatcagcga aggttgaact
     2281 ctgacgcact gtggacgccg agctaataat catcgctatg gttcccggac cagcggtact
     2341 tggcattgct agcggcacaa aggcaatatt ggcactgggt tcatcttcca gctcttccga
     2401 cttgcttttc gcctccggtg aatcaatcgc tttctgttgc ggaaagagca tccgaaaacc
     2461 gataaacgcg acgattaagc cgcctgcaat tcgcagaccg ggaatcgaaa tgccaaatgt
     2521 atccatcacc agttgcccgg cgtaatacgc caccatcatg atggcaaata cgtacaccga
     2581 ggccatcaac gactgacgat tacgttcggc actgttcatg ttgcctgcca ggccaagaaa
     2641 tagcgcgaca gttgttaatg ggttagctaa cggcagcaac accaccagcc ccaggccaat
     2701 tgctttaaac aaatctaaca ttggtggttg ttatcctgtg tatctgggtt atcagcgaaa
     2761 agtataaggg gtaaacaagg ataaagtgtc actctttagc taaccttgca tcgcattgac
     2821 caaaacttga accgatttag caaaacgtgg catcggtcaa ttcattcatt tgacttatac
     2881 ttgcctgggc aatattatcc tctgcaacta attacttgcc agggcaacta atgtgaaaag
     2941 taccagcgat ctgttcaatg aaattattcc attgggtcgc ttaatccata tggttaatca
     3001 gaagaaagat cgtctgctta acgagtatct gtctccgctg gatattaccg cggcacagtt
     3061 taaggtgctc tgctctatcc gctgcgcggc gtgtattact ccggttgaac tgaaaaaagt
     3121 gttgtcggtc gacctgggag cactgacccg tatgctggat cgcctggtct gtaaaggctg
     3181 ggttgaaagg ttgccgaacc cgaatgataa gcgcggcgta ctggtaaaac ttaccaccag
     3241 cggcgcggca atatgcgaac aatgccatca attagttggc caggacctgc atcaagaatt
     3301 aacaaaaaac ctgacggcgg acgaagttgc aacacttgag catttgctta agaaagtcct
     3361 gccgtaaaca aaaaagaggt atgacgatgt ccagacgcaa tactgacgct attaccattc
     3421 atagcatttt ggactggatc gaggacaacc tggaatcgcc actgtcactg gagaaagtgt
     3481 cagagcgttc gggttactcc aaatggcacc tgcaacggat gtttaaaaaa gaaaccggtc
     3541 attcattagg ccaatacatc cgcagccgta agatgacgga aatcgcgcaa aagctgaagg
     3601 aaagtaacga gccgatactc tatctggcag aacgatatgg cttcgagtcg caacaaactc
     3661 tgacccgaac cttcaaaaat tactttgatg ttccgccgca taaataccgg atgaccaata
     3721 tgcagggcga atcgcgcttt ttacatccat taaatcatta caatagctag ttgataacgt
     3781 gacaacgtca ctgaggcaat catgaaacca ctttcatccg caatagcagc tgcgcttatt
     3841 ctcttttccg cgcagggcgt tgcggaacaa accacgcagc cagttgttac ttcttgtgcc
     3901 aatgtcgtgg ttgttccccc atcgcaggaa caaccaccgt ttgatttaaa tcacatgggt
     3961 actggcagtg ataagtcgga tgcgctcggc gtgccctatt ataatcaaca cgctatgtag
     4021 ttattgtcag gctctgatca cagagcctga catctccgta atatcttcct caagtctcaa
     4081 atattttcgc cattgttggc gatgagatcc cgataccagt aaaacgacgc tttacgtatc
     4141 cgttccagcg ttcccttccc ttcgttgtct ttatcgacat aaatcattcc gtagcgcttt
     4201 ttcatttccc ctgttccggc agaaaccagg tcaatgcagc cccacggggt gtagccaatt
     4261 aagtcaacac catcttcaac tacggctttt ttcatttccc gaatatggga agcaaagtaa
     4321 tcaatgcgat agtgatcgtt caccgtgccg tcagcttgtc tctggtcaac cgcaccaaat
     4381 ccattttcga caataaacag cggcaactgg aaatgatccc agaaccagtt gagtgaataa
     4441 cgtagccctg ccggatcaat ttgccatccc cattcggatg tatcgatata agggttgctg
     4501 accaaatctc gtggttcaac ataatccagt tgcgggttat cgggagaaaa ttgtgtcgta
     4561 aaagacatgt agtagctaaa accgataaag tcgacacatc cactggcaag aatcgcgtta
     4621 tcttctggtg tgatatcgag gttgaatcct ttcctggcaa agtaattcag catatgttgc
     4681 ggataatatc cacgagcatg aacatcagta aaccagtaac gacgatgcat cgctttcgtg
     4741 gccatcatca tatcgttggg tgcacacgtc agaggataga tggggcacat ggcgatcata
     4801 cagccaatgt taaattcagg attgatcgat tttccagtct gtaccgccag ggcactggca
     4861 actaactcgt aatgtaccgc ctggtacatt atttgttcgc gctcattgat atcttcctct
     4921 ggcgaataca agataccgga attagtaaat ggacacaggc tttcgctgaa attcacctga
     4981 ttattgattt cgttaaacgt catccagtac tttacttttt ctttatagcg cgtgaagacc
     5041 gttgatgcga agcggataaa aaagtcgatc agcttacggt ttcgccagcc accatatttt
     5101 gtcaccagat gataaggcat ctcaaaatgc gaaagcgtca ccacaggttc cattccttgc
     5161 ttcaggcatt catcgaacag atcatcataa aattgtaaac cctcttcatt cggctcctgt
     5221 tcgtcacctt gcggaaagat tcgtgtccag gcaatggaag ttcgaaagca tttgaatccc
     5281 atctcggcaa ataactgaat atctgtttta tagcgatgat aaaaatcaat tgcttcatga
     5341 ttgggataat taagcccgtc gataacgcct tctgtcactt cacgcggcac cccgtgagcg
     5401 ccagcagtca tcacatcagc gatactgatg ccttttcctc cttcattcca gccaccttcc
     5461 aactgatgcg cggctaccgc gccaccccat aaaaaacctt ttttaaatcc tgacatacac
     5521 aactccttaa ctaaaataga ttaccaccag acttcc
//
LOCUS       DAZRUF010000244         5541 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZRUF010000244 DAZRUF010000000
VERSION     DAZRUF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.245"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..1369)
                     /locus_tag="ACHXOL_003676"
     CDS             complement(<1..1369)
                     /locus_tag="ACHXOL_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR6810552.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWL"
     gene            complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHXOL_003677"
     CDS             complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHXOL_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR6810553.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHXOL_003678"
     CDS             complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHXOL_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR6810554.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHXOL_003679"
     CDS             complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHXOL_003679"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR6810555.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHXOL_003680"
     CDS             complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHXOL_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR6810556.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1410 a         1450 c         1304 g         1377 t
ORIGIN      
        1 atagccattg ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc
       61 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg
      121 gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca
      181 agggaattgc taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga
      241 tttgcacggg cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg
      301 cacgcaatac cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca
      361 gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca
      421 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac
      481 ttaacttaag cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca
      541 ttcccgtcaa ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc
      601 agtggatcca gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca
      661 acaccgcaaa agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc
      721 gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa
      781 taaccgcatt ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
      841 gcaggctaca attcggttca ataacacttg cctgcagaaa gcggccggtg agcggctgat
      901 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg
      961 cacgaacttt gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt
     1021 cgccatacag cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt
     1081 gaccgtaatg agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg
     1141 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga
     1201 aaaacagcaa cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc
     1261 aggcgaagag agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca
     1321 acggaaaaat tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc
     1381 tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc
     1441 gaggcacatt cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag
     1501 cttccagttc ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca
     1561 agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt
     1621 aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc
     1681 ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc
     1741 gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt
     1801 cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat
     1861 gattcctccg gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc
     1921 ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact
     1981 caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt
     2041 gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc
     2101 gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt
     2161 tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt
     2221 agcgtcttca acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc
     2281 agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc
     2341 gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc
     2401 cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc
     2461 ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg
     2521 aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt
     2581 gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt
     2641 agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac
     2701 cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt
     2761 ccaatccatt tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca
     2821 gccgtagtca gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt
     2881 agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg
     2941 ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct
     3001 cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc
     3061 cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc
     3121 ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc
     3181 acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag
     3241 ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg
     3301 caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat
     3361 aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg
     3421 acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag
     3481 agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg
     3541 ctcagccggg atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc
     3601 gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga
     3661 ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg
     3721 tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac
     3781 gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc
     3841 gctttttcaa tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct
     3901 gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg
     3961 gccaaaaggc gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga
     4021 tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc
     4081 accgttccca tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg
     4141 cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc
     4201 acttcctgag tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg
     4261 ccattggttg acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct
     4321 gacgcaacat caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc
     4381 gaatccagca gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct
     4441 gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta
     4501 aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta
     4561 acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata
     4621 gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg
     4681 atcacggatg tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt
     4741 gcgaccgcca tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat
     4801 gccacgtttc ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga
     4861 gtatttagca ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga
     4921 agccacaccg ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt
     4981 catttcagcc agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc
     5041 aatttcgttg tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc
     5101 catcgcccat gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt
     5161 aatattgtta cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt
     5221 acttgcgact tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc
     5281 atcggcatcg cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt
     5341 ggtatcaata ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc
     5401 cagcagtaat ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta
     5461 tgtatcaaca acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc
     5521 cgaaaaaaaa cgccgacaat g
//
LOCUS       DAZRUF010000245         5527 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZRUF010000245 DAZRUF010000000
VERSION     DAZRUF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.246"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHXOL_003681"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHXOL_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR6810557.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHXOL_003682"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHXOL_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR6810558.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="ACHXOL_003683"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="ACHXOL_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR6810559.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHXOL_003684"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHXOL_003684"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR6810560.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHXOL_003685"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHXOL_003685"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR6810561.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="ACHXOL_003686"
     CDS             4447..4812
                     /locus_tag="ACHXOL_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR6810562.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="ACHXOL_003687"
     CDS             4962..5480
                     /locus_tag="ACHXOL_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR6810563.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1519 a         1390 c         1241 g         1377 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggtgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatgtttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAZRUF010000246         5501 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZRUF010000246 DAZRUF010000000
VERSION     DAZRUF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.247"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(432..3974)
                     /locus_tag="ACHXOL_003688"
                     /pseudo
     CDS             complement(432..3974)
                     /locus_tag="ACHXOL_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4042..>5501)
                     /gene="tssI"
                     /locus_tag="ACHXOL_003689"
     CDS             complement(4042..>5501)
                     /gene="tssI"
                     /locus_tag="ACHXOL_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR6810564.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGSNQII
                     KVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKV
                     GNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKE
                     QVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
BASE COUNT          958 a         1802 c         1454 g         1287 t
ORIGIN      
        1 tcttcaaaaa acttcattgg agaatctttg atattctcaa tcttttcacc ataaacgtct
       61 aatattgatg gttcaccagg gtcatcaaac tgctcaccat aaggtaaaat acccaataaa
      121 tttctccaac acctatctct accattttcc tgaagtgatt ctgataattg taaatttgct
      181 ctgttatatg cagctgtcgc agattttata ttccacgccc ctaaagccat agaagctgca
      241 ccgactgggg cagcaatgat cgttccatcg gcagcaacac ttataccgaa ttttgtcatg
      301 ccacttgcat acagccttgc tgaattagca gtatttaaac tagctgtaaa catttttgtc
      361 gggttaaacg gaaggttatc cagtttatta tttatatcat ctataaaatg actacaactc
      421 tcacatgaga ataaccccaa cggatctatc ctgctcaccg gattcagcgg atactgatac
      481 agattccacc cacctttcag cccaatgggg tcctgggtga tgtaccgtct ctgcaacgga
      541 tcatagtacc ggtgacgatt gtagtacagc cccgactcct catcatactg ctgccccggc
      601 agacggtatg gctgatacac atgatgcggg ttctcctcat ttagctggtt gccccactca
      661 tcatacgccc cgctccacac cgtatcgccg tcttcgctga tgagcgccag cggctggccc
      721 cgatggtcgc agtggtaaag atgaacgttt cgcactggtg tgtattccgg ctccacctgt
      781 ctggccagtt gctccaccgt cagcccgcac tgcgcaagcc acacccggct ttcactgctc
      841 acgcggtctg cccggatttc ttcctccagt ctgtccagca gccgcaccag ttcagccgga
      901 aataccacgc cgtgcccgtc ctcgctccct tcctgctgca gcttctccgc caggctgcgg
      961 cgctgcgctt tctcccgctc gccgttctct gtctcgaccc ggatgagcgg cgtgaagctt
     1021 cccggctcgt ataccgtctg gatacgtgtg gtgtcggtct gtaccgtcgt cagcctgtcg
     1081 ccgtcccagc cgtaccatgt ctcctccggt ttacgcgaca gcgacatcca ccccgtcagg
     1141 tcacgctccc gccgccatac ccgtttcgcc attcgccgtc ccagcgggtc gtagaggtag
     1201 cggctctcga ccagtggctc gccatgctgt atccgcgtgt ggaacaccag gcggtgctgg
     1261 ctgtcgtagt ggtgggtccg ctcgtcgtcc gtccgtatca cacccgtcgg gatgcggtcc
     1321 gtcttctccg tcagtctgcc gtattcatca tagtggtaaa catagtgcgc atcctctgcg
     1381 atgcggttat ccggccacgc cgtgagcgtg ctgtccgggt gtagctccgg gtccggcagg
     1441 cggtttcccg ccgggtcggt ggcatacggg atgcggatgt ccaggtccgg tgcgagggtg
     1501 cgcaccgtct cacggtgcag cctgtcccgc gtatactcga ccagcggcgt cccgcccagc
     1561 ttcatgcccg ccaggtaacc gctgccgtag gtcagccact ccaccggcgg caggctgtcc
     1621 ggcgtgacgc ggtttgccag cccctgttcg ttgtaggcgt gtttcgtctc atgctgccac
     1681 agcagttccc ccgtctccgg gttctccacc gtctgccgtt cgcccgtcag gcggccctta
     1741 tcgtcgtagc cgtagtggac agcgacacgg tggccttcgc tcaggtggct gatttctctc
     1801 agccagccgt ggccgtcata ctgccactgc tctgccgggt cgccgttcac cgtccggtgc
     1861 gtgatgcggt ccgacgcatc gtagtgccag agggtgacaa gcccctcatc ctcactctgc
     1921 gtgagttttc cggtcaggtc ataatgataa cgttgcgtcc gcccgtcaaa gccgccctgc
     1981 tgtaccagcc ggtccagcgc atcgtagctg aagtcgctgt gactgccgtt ctcgttagtc
     2041 agactgatga cacgtccggc agcatcgtac tccatactgc gcgtcagccc gccctgcgtg
     2101 gtgctgaccg cctttcccca cgcatcgtac tgtgtctcgc tccggttgcc gtccggggtg
     2161 ataacggcag tcaggtcgcc tgcggcgttg tactcatact gcgtttcacg gccctgtgcg
     2221 tctttcaccg aggttaaccg gccacggttg tcatagtggc ggtaaaggct gataccttcc
     2281 tcgcggtgaa ccgccgtcat ctggccgaag cggtcgtatt catagcgggt ctggtagccc
     2341 gagcagtcag tgaacgccag caactgcccg tagcggctcc acgtcatctg ccgggtgctg
     2401 cccgtcgcat ccgttgtcgt cgccggtaac tcactgtgcg cgtcatcgta gcggtagcgt
     2461 actgtctccc cgctgcgcga tgtctccgat accagcctgc ccggttcatc atattcccgg
     2521 cggctctcca gcccgtccgg gtacaccacc gccgtcagct ggttcccgtc gttatagtaa
     2581 aatttcgtct cccgcccgtc cggtgtggtg atgtccgtga tatcgccgga caccacattc
     2641 agaccgtact ctgtccgccg tcccgccgcg tccgtctgcg ccgtgagcct tcctgccgcg
     2701 tcatacccgc tgcgcgtgac gctgccgtcc gccagttctt ttttcaccac ccgtttcagc
     2761 ccggccccgc cttctgtatg cagcacctca cgccggttca ggctgtcggt gacggtgatg
     2821 cggtcctgct cataaagata gcggtagctt aaccctgccg ggttcagttg ctccaccacc
     2881 cgccccgtat cgtcgtagcg gtagcgcatc tccggccttc ccgcgtaacg gtgcgccacc
     2941 atccggcccg ggtgctgcgc gtcatacgtg aaagcgcgca cctgcgtatt gctgcggtca
     3001 tataccgcca gcagttcacc ggcttccgta tacgtgtacc gcaccagtgg cgcagcgggc
     3061 aggctctccg ggtatgccgg gtcgtgcatc aaccacaccg ccgaaaggcg gatacccctg
     3121 tcggggccgt attcggtacc aggcagtgtg tcggggaacg ctgaggctga gagagggcgg
     3181 gaactgtcag aagaagatag cgaagaggtg cgggcctctt ccgcacgctg cgcctgcgtg
     3241 gtcagcacca gacggaactc ccgcccggca ccgtccgtca cgtcggtgat ttccccggcc
     3301 aggtcaccgg cggcctcacg ccggtacgtc agcgtccgcc atcccggtaa gcacccggta
     3361 cggcggcagc ggcgctggca gtacgtcctc agcacccggc acccgctcag ccccccccag
     3421 tatccaccac ggcccctgtg cgctgttggt cgccaggtaa agatgcgggc ttaaccggat
     3481 atccggcgca gcgcccccca cagccgcgcc agcgtatggc cgtccggctg tgctgcctta
     3541 ccaccgcgca ccagccacat tgactcactg cggctgtaca ccgcctcccc cggcagcagc
     3601 ggctcaaagt gaatgctccg cccgccgttg tcgttgagta tcagtccgtc atcacgtagc
     3661 tgtaagcgga tatcagaagg cgctttccag ccggggccga aaacgcccac cggtgcaggc
     3721 gtcttcgtcc ggtagctgct gtaggtgcgg gagagaatga acggcagcgg gccgggcagc
     3781 gcaaggtccg tctcgccggg cagcaccttc gcccccagca gcggatttac cgggttgccc
     3841 gaagtcatcc cgcccggaca caccgagcac gccacgccgg tgggcgcgcc aattcttaca
     3901 cctgccgaac cctggacaat gggaccgcca tactgagtca tatctccctg acgcgccgct
     3961 ggttttccgc tcatgattca aattccttct tctgtttatt tttaatgctg gtttgttact
     4021 gaataccgaa caggtacggg atcagtactc tctcatatcc ggcgtatcct gcttttcatc
     4081 aggggtcttc ggtggggatt tcgaggctgc acagttccag ttaatcaaca gtgagtcacc
     4141 attaacctgc tccttaccct gcaaatgaat ttttgtgccg ttgagaaaaa tttgtccgtc
     4201 cttcgtcagc accagccttg ccttaccaca gcagagctcc agatgctcac cggcggagta
     4261 aatcgcggtc tgcccggtat catccttttt cgttttacca acggtgaagg taacgttatt
     4321 tccgactttg acgtcataac ccagcccgac cctcaacgat ttatgcaaac cgatctgcga
     4381 ggactgcatc agtgccaccg aggtgttcat aatgccacct accgtcgtct gatacgccac
     4441 gcccaccgtc agcgcacgaa caagtccaat cgtttccacc tgaacagaac ccactttaat
     4501 tatctggtta ctgcccaccg tctctatctg attgttggta accgcaatca tctgattgtt
     4561 gccaatggtt tcagcatggt tgtttatcac atccgtggtg cggttattca gcacctcggt
     4621 gttcatgttc ttctgcgcgt ggatgtagac ctgttctttc cctgtcgcat cgtcaaactt
     4681 cagttcatta aacccgctgc ccttataggt tttcgaacga atggtcatct gcgtcttcgt
     4741 ccccggcagg ctgccgggtg tgcggttttc ctggtggtag gtacgcccca taataatcgg
     4801 ctggtccggg tcgccgttga ggaagtccac aatcacctcc tgacccacac gcggtatcgc
     4861 caggttgcca aagccggtgc ctgcccacgc ctgtgccaca cggatccagc atgaactgtc
     4921 ctggtttgac gggttatagc ggtcccagtt aaatttcacc cgcacgcggc catgttcatc
     4981 acagaagatt tcctcgcctg ccggtcccgt cacgacggcg ctctgcgggc catccaccag
     5041 cggtttcatc aacggctgtg gccgccatgt tctgtctgcc gggattaccg caaaatggtt
     5101 ctccagcgtg gtgcctgaac ccctgcgtcc cggtaccgcc tgcggctgtt cgccgtgaag
     5161 atcgctcgcc accacctgcc attcccggtt caggttcgcc tgcggatgcc ccgtcagcac
     5221 aattcgccgt cccggccata tctccggcga acggcttgtt ccgcgcgcca cttctgcgtt
     5281 gtttcgccag ccgtccatct gccagcgggc aaagttctgc ccgtgggcac ccttgaaacg
     5341 tccggggtag tcatacactt catactgcgt gcgctggtaa tcctggtact ggccttcctg
     5401 atcaaaacgc cctggccagc cgggacgttt aaaggtgtag tctttggtca ctacggaaga
     5461 aggacggatt tgtgcgctgt agcgaaactg gctgatgcag a
//
LOCUS       DAZRUF010000247         5341 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZRUF010000247 DAZRUF010000000
VERSION     DAZRUF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.249"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHXOL_003690"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHXOL_003690"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR6810565.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHXOL_003691"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHXOL_003691"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR6810566.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHXOL_003692"
     CDS             complement(2698..3390)
                     /locus_tag="ACHXOL_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR6810567.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHXOL_003693"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHXOL_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR6810568.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1366 a         1380 c         1266 g         1329 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 t
//
LOCUS       DAZRUF010000248         5314 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZRUF010000248 DAZRUF010000000
VERSION     DAZRUF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.250"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(65..466)
                     /gene="crl"
                     /locus_tag="ACHXOL_003694"
     CDS             complement(65..466)
                     /gene="crl"
                     /locus_tag="ACHXOL_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR6810569.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(524..1768)
                     /gene="frsA"
                     /locus_tag="ACHXOL_003695"
     CDS             complement(524..1768)
                     /gene="frsA"
                     /locus_tag="ACHXOL_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR6810570.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(1860..2318)
                     /gene="gpt"
                     /locus_tag="ACHXOL_003696"
     CDS             complement(1860..2318)
                     /gene="gpt"
                     /locus_tag="ACHXOL_003696"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR6810571.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2579..4036
                     /gene="pepD"
                     /locus_tag="ACHXOL_003697"
     CDS             2579..4036
                     /gene="pepD"
                     /locus_tag="ACHXOL_003697"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR6810572.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(4093..4707)
                     /gene="prfH"
                     /locus_tag="ACHXOL_003698"
     CDS             complement(4093..4707)
                     /gene="prfH"
                     /locus_tag="ACHXOL_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR6810573.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(4704..5314)
                     /locus_tag="ACHXOL_003699"
                     /pseudo
     CDS             complement(4704..5314)
                     /locus_tag="ACHXOL_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
BASE COUNT         1303 a         1425 c         1306 g         1280 t
ORIGIN      
        1 cggttggcat gacctatcag ttttaatgga tattgccgga tgtgatacat ccggcacatt
       61 tcactcacgc cgttaacttc accggttcat cacgaaaatc atccgccggt tccagcttca
      121 gattcagcgt cgtcagcagt tcacgcagct tctcgtgaaa ctcacgtagg gtgtgctcca
      181 gtcgttcaac cacttcagtg tcttttaccg gaacactctt ccagtcgcct gctttatcga
      241 acagaccaaa ctggtaactg taggtaaaac gggattcctg cgcttcaagc tccatccacc
      301 agccccagaa ctcacgcact tccggtgccg gtttcacgtt gacgcataca gccagacaat
      361 cgaaaaagaa tcgattatct tcgcacttac cttcacgaat atacgggcct agtgcggtaa
      421 attttttgat caatctgctc ttcgggtgtc cactcggtaa cgtcattgca atctcctgtt
      481 gtgatgcaac tgttttacca aattggcaaa ttttagcaaa tttttaacac aagcgttttt
      541 cgatccagcc ggtgatttcc tgaagacctt tgtcaaaatt ccgatacacc gggttaaatg
      601 ggatctctaa taatttaccg tcagcagatg atgaggtgat taagcgtgag tcctcttccg
      661 ggctgaacgg atcgttcttc cagtagcctg ataacattgg cgttgggcag cgacgcccca
      721 gcaatccttg cacttttaat gaatagcgat tcagctccac gcgtaacgct tcgtcggaag
      781 catcatgcat ccccaaacga ctcgccagaa cgtcaagata catttccggc acctgttgct
      841 ggcacttaaa atcactcaac agggtatgaa ctaccggacc aagacaggca accgctttca
      901 gacgcggcga ttcaagatat gccagacgca cggcaacgtt agcgccgaaa cggaaaccaa
      961 aggccgcgac gcgagtgtga tccacccacg gtacgttagg cagagccttt aaaacgtgct
     1021 gatgcaacag gctggagtcc tgggtgagct tccattttga tgaaaagccc accgacggca
     1081 tatcaatagt cagcatcgca atgccgcgcg gcgcaaaata acgttcatac aggctgtaat
     1141 agtccgtctg catcgcatcc agaccaccac acattaatac tgtcgggaac gggccatcgc
     1201 ctttcggcat atgcaaaaag ccggtgatgg gcgcaccgcc gggtacggta aactccatct
     1261 gccgcatcgt gcccggcaga cgctgggcgg cttcttcata ggcacggttt gacaaagcct
     1321 gcgcttgctc ggccaggtca tctcctttca gatgaggata ggcagcaatg ttgtacaacg
     1381 tagccgcatg cagccagtga cgaccactga gttgcggatc ttcttccgca caggcttttt
     1441 gttgccacac catcgcctgg atggcccact cataaatcca gttgccacca cggtagccaa
     1501 tcaccgtatc gtataaatca tcgtcggtac gttcggcatc gctcatcaca atacgcgcct
     1561 ggacgtcgag gatttcacgt gggtcaatgc cgcgccagat ccacatcaga cggttaatca
     1621 tgcgatacca gtgagggatg gttttaccat caagggccga ctgcacaggc ggttgtgcgc
     1681 cgtgattaaa gcggcggact agcgtcgagg tttcaggatg tttaaagcgg ggtttgaaca
     1741 gggtttcgct caggtttgcc tgtgtcatgg atgcagcctc cagaatactt actggaaact
     1801 attgtaaccc gcctgaagtt aaaaagaaca acgcccggca gtgccaggcg ttgaaaagat
     1861 tagcgaccgg agattggcgg gacgaatacg acgcccatat cccacggctg ttcaatccag
     1921 gtatcttgcg ggatatcaac aacatagtca tcaaccagcg gacgaccagc cggttttgcg
     1981 aagatggtga caaagtgcgc ttttggatac atttcacgaa tcgcaaccgc agtaccaccg
     2041 gtatccacca ggtcatcaat aacgatgaag ccttcgccat cgccttctgc gcgttttagc
     2101 actttaagct cgcgctggtt gtcgtgatcg tagctggaaa tacaaacggt atcgacatga
     2161 cgaataccca gttcacgcgc cagtaacgca cccggtacca gaccgccacg gcttacggca
     2221 ataatgcctt tccattgttc agaaggcatc agtcggcttg cgagtttacg tgcatggatc
     2281 tgcaacatgt cccaggtgac gatgtatttt tcgctcatgt gaagtgtccc agcctgttta
     2341 tctacggctt aaaaagtgtt cgaggggaaa ataggttgcg cgagattata gagatctggc
     2401 gcactaaaaa ccagtatttc acatgagtcc gcgtcttttt acgcactgcc tctccctgac
     2461 gcgggataaa gtggtattct caaacatatc tcgcaagcct gtcttgtgtt gacaacattt
     2521 tctgctaacc ctgtgacctg caatactgtt ttgcgggtga tcgacaagga gacttaacgt
     2581 gtctgaactg tctcaattat ctccacagcc gctgtgggat atttttgcca aaatctgttc
     2641 tattcctcac ccgtcctatc atgaagagca actcgctgaa tacattgttg gttgggcaaa
     2701 agagaaaggt ttccatgtcg aacgcgatca ggtaggtaat atcctgattc gtaaacctgc
     2761 taccgcaggt atggaaaatc gtaaatcggt cgtcttacag gcccacctcg atatggtgcc
     2821 gcagaaaaat aacgacaccg tgcatgactt cacgaaagat cctatccagc cttatattga
     2881 tggcgaatgg gttaaagcgc gcggcaccac gctgggtgca gataacggca ttggtatggc
     2941 gtctgcgctg gcggttctgg ctgacgaaaa cgtggttcac ggcccgctgg aagtgctgct
     3001 gaccatgacc gaagaagccg gtatggacgg tgcgttcggc ttacagagca actggttgca
     3061 ggctgatatt ctgattaaca ccgactccga agaagaaggt gaaatctaca tgggttgtgc
     3121 ggggggtatc gacttcacct ccaacctgca tttagatcgt gaagcggttc cagctggttt
     3181 tgaaaccttc aagttaacct taaaaggtct gaaagggggt cactccggcg gggaaatcca
     3241 cgttggcctg ggtaatgcca acaaactgct ggtgcgcttc ctagcgggtc atgcagaaga
     3301 actggatctg cgccttatcg atttcaacgg cggcacactg cgtaacgcca tcccgcgtga
     3361 agcctttgcg accattgctg tcgcagctga taaagtcgac gtcctgaaat ctctggtgaa
     3421 tacctatcag gagatcctga aaaacgagct ggcagagaaa gagaaaaatc tggccttgtt
     3481 gctggactct gtagcgaacg ataaagctgc cctgattgcg aaatctcgcg atacctttat
     3541 tcgtctgctg aacgccaccc cgaacggtgt gatccgcaac tcagacgtgg caaaaggtgt
     3601 ggtcgaaacc tccctgaacg tcggtgtggt gaccatgact gataataacg tagaaattca
     3661 ctgtctgatc cgttctctga tcgacagcgg taaagactac gtggtgagca tgctggattc
     3721 gctgggtaaa ctggctggcg cgaaaaccga agcgaaaggc gcatatcctg gctggcagcc
     3781 ggacgctaat tctccggtga tgcatctggt acgtgaaacc tatcagcgtc tgttcaacaa
     3841 aacgccgaac atccagatta tccacgcggg cctggaatgt ggtctgttca aaaaaccgta
     3901 tccggaaatg gacatggttt ctatcgggcc aactatcacc ggtccacact ctccggatga
     3961 gcaagttcac atcgaaagcg taggtcatta ctggacactg ctgactgaac tgctgaaaga
     4021 aattccggcg aagtaattat ttgatttgct gccggatggc gtttaatcgc cttccggcag
     4081 tttcatcctt cattatcctt cgataaaagc catccctgta aatgtccgtc tcgggttgcc
     4141 acgttcaatc tggtgatgga acattcgccg ctgcgatttc agcaccgcgc tattttcctg
     4201 ttgctgctgc tccagcttcc aggcaatcag caatcgtgcc agccgcttgt tagcatgctg
     4261 actacgctct gactgaacct tcacgctaat accggatgcc aaatgcgtgg cgcgtaccgc
     4321 cgagtcggtt ttattgacat gttgaccgcc cggccccgac gaacgcaacg tctcataacg
     4381 gattgcatcc gattgttcct gctcatcagc ggtaaaacgc ccaatgccca gaaaccagtt
     4441 tttgcgccca tgatgaggcc gatacggact cggacaaatc cactgaatag tgccgcacca
     4501 gctttcgctt aatgcccatg cgttatcgcc atccagagaa accagagctg aacgcagcgt
     4561 gtcagagtag cggcccgttt ctgtttccaa caccgttacc gcgacgtctt gtcgggcagc
     4621 ttctttaatt aatcgctcca gcgccttttt cactgcgaga caacattctt ccggcccctg
     4681 cgctgaggag agttgtagca agatcatcct tttttccctc cactgttttt gagcgttagc
     4741 accggacgca gccgcgcgac aggaataatt aaaccagcct gcaccagaca ttgcaccacg
     4801 ctttcagccg atttataggc ttgtggcgct tcttcaaaga tgagttgttt atcgcgacaa
     4861 attacccgac tgccaagttc agtacgtgag agctgcgtcg ctgtgtattt cgctgccaga
     4921 cgccctttac actcggtgcg cccccattta cgcccagccc catgcgccag cgagtggagc
     4981 gttttttcgt tcgcaacagg ctgtacaagc taggagtaat caccgagtga accgggaata
     5041 atcaccagac cgttgtcatc cggcgtggca cctttacgat gcaaccagcc ctgttgatcg
     5101 ccgatttgac acgcgctaac aaagttatgc gccacatcca gaaccggact tccggtggcc
     5161 ttaacctgtt gcattatgcg taaagagatc agctggcgat taatacgcgc aaacgccagc
     5221 gcatcgtcat gttccgcaat ataccgtagc gcgtcgtcac tgccttcagg caaaccatga
     5281 tgcgaaaacg aggcaatatg ccgctgtaaa atag
//
LOCUS       DAZRUF010000249         5240 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZRUF010000249 DAZRUF010000000
VERSION     DAZRUF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.251"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..69
                     /locus_tag="ACHXOL_003700"
     CDS             <1..69
                     /locus_tag="ACHXOL_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810574.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
     gene            complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHXOL_003701"
     CDS             complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHXOL_003701"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR6810575.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHXOL_003702"
     CDS             complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHXOL_003702"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR6810576.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHXOL_003703"
     CDS             complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHXOL_003703"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR6810577.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHXOL_003704"
     CDS             complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHXOL_003704"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR6810578.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPLEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHXOL_003705"
     CDS             complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHXOL_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR6810579.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         1133 a         1395 c         1393 g         1319 t
ORIGIN      
        1 gtctactggg taattgcggt tatttctgtt gtcgcattat ttttaaccgc aaaggttaaa
       61 gacgtttgat gacgtggacg atagcggaaa gcccggtcat ttgaccgggc aaggggatta
      121 attcataaac gcaggttgtt ttgcttcata agcggcaatg gcgtcgtcgt gctgcaaggt
      181 aagcccaata ctgtccagac cgttcatcat gcagtggcgg cggaaggcat cgatggtaaa
      241 gcgataggtt ttctctcccg ctttcacctc ttgcgcttcc agatccacgt cgaaatggat
      301 ccccggattg gctttcacca gcgcaaacag ttcgtccact tctgcatcgc ttaatttcac
      361 cggcagcagc tggttgttaa agctattgcc gtagaagatg tcagcaaaac tcggcgcaat
      421 caccacttta aagccgtagt cggtcaatgc ccagggcgcg tgctcacgcg aggagccaca
      481 gccgaagttt tctcgtgcca gcaaaatgga agcgccctga tactgcggga agttcagcac
      541 gaagtccggg tttggctgtt ggcctttttc atcgagaaaa cgccagtcgt taaacagatg
      601 cgcgccaaaa cccgtacggg tcactttctg caaaaactgt ttcgggatga ttgcatcggt
      661 atcgacattg gcggcatcca gcggaaccac caggcctgtg tgtttgataa atttctctgc
      721 catggtgtgc tccttattta atgttgcgaa tgtcggcgaa atgtccggtc acagcagcag
      781 cggcagccat tgccgggctg accagatgcg tgcgcccgcc gcgcccctgg cggccttcaa
      841 agttacggtt gctggtggat gcacaacgtt cgcccggatt cagacggtcg ttgttcatcg
      901 ccagacacat tgagcagcca ggcaagcgcc attcaaaacc ggcttcaata aagattttat
      961 ccagaccttc cgcttccgcc tgggctttta ccgggccaga gccgggaacc accagtgcct
     1021 gcacgcctgg cgcgactttt cgccctttgg cgatctccgc tgccgcgcgt aaatcttcaa
     1081 tgcgcgagtt ggtacaggaa ccgataaaca ctttgtcgat agccacttcg gtcagcggaa
     1141 tgcccggttt cagccccata taggccagcg ctttttctgc cgacgcgcgt ttaaccggat
     1201 cggcaaacga agccggatcg ggaatattgt cgttcacgga aatcacctgg cctggattgg
     1261 tgccccaggt gacctgcggt gaaatttctt ctgcttgcag agtgacaacg gtatcgaaag
     1321 ttgcgccttc gtcggtttgc agggttttcc agtaggcaac ggcgtcgtcg aaatctttgc
     1381 ctttcggcgc atgcaaacgg cctttgacat agttaaaggt ggtttcgtcc ggtgcaacca
     1441 gaccggcttt tgcgcccatt tcgattgcca tattgcacag ggtcatacga ccttccatgc
     1501 ttaaatcacg gattgcttcg ccgcaaaact ccaccacatg cccggtgccg cctgcgctac
     1561 cggttttacc gataattgcc agcacgatat cttttgcggt aatgcccggc gcggctttgc
     1621 cctggacttc aattttcatg gtttttgcgc ggccctgttt cagggtttgc gttgccagta
     1681 cgtgttcaac ttcggaagtg ccgataccaa aggccagtgc gccaaacgcg ccgtgggtgg
     1741 cggtatgcga gtcgccgcag acaatggtca tccccggcaa ggtgacgccc tgttccggcc
     1801 ccattacgtg gacgatcccc tgatacgggt gattcaggtc atacagttcg acgccaaatt
     1861 ctttgcagtt tttaatcagc tcctgcatct ggatgcgcgc catttcacca caggcattaa
     1921 tgtctttcgt ctgggtagag acgttgtgat ccatagtggc gaaggttttg cccggctgac
     1981 gtaccgggcg accgtgggcg cgcagaccat cgaacgcctg cggtgaggtc acttcatgca
     2041 ccaggtggcg gtcgatatat aacagcgggg tttcgttttc ggcttcgtac actacgtgag
     2101 cgtcgaataa tttttcgtat aacgttttag ccatgattac accccttctg ccacatagcg
     2161 ggcaatgata tcgcccattt catcggtact aacggcggca gcgccacggg ctaaatcccc
     2221 ggtgcgaatg ccttcttcta atgcgcggtt aatggcgcgt tcaatggcgc aagccgcatc
     2281 atcggcatcc aggctgtagc gcagcagcag tgccagcgac agaatttgcg caatcgggtt
     2341 ggcgatgttt ttgcctgcga tatctggtgc tgagccgcct gccggttcat acagaccaaa
     2401 accttgctcg ttcaggctgg cggaaggcaa catccccatc gagccagtga tcattgcgca
     2461 ctcgtcagac agaatgtcgc caaacaggtt ggagcacagc agaacgtcaa actgtgatgg
     2521 atctttaatc agctgcatgg tggcgttgtc gatgtacata tgcgccagtt cgacatccgg
     2581 gtattccgtg gcgatctcgt tgacgatttc acgccataaa atagaggatt gcagcacgtt
     2641 agctttgtcg attgaggtga ctttgtggcg acgtttacga gcagattcaa acgcgatgcg
     2701 ggcaatacgc tcgatctcaa aacggtgata cacctcggta tcaaacgctt tttcatattg
     2761 tccgctacct tcgcggcctt ttggctgacc gaaatagatg ccgccggtca gttcgcgcac
     2821 gcacaggatg tcgaagccgt tagcggcaat gtctgcacgc agcggacaga atgcttccag
     2881 cccctgatac agttttgccg ggcgcaggtt gctgaataat ttgaagcgct tacgcagagg
     2941 cagcagcgcg ccgcgttctg gttgctggtc tggtggtaaa tgttcccact tcgggccgcc
     3001 taccgagcca aacagcacgg catcggcttg ctcacaacct tcaaccgtcg caggcggcag
     3061 tggttgcccg tggttatcaa tggctgcgcc gcctacatcg taatggctgg tggtgatgcg
     3121 catcgcaaag cggttgcgca cggcattcag cactttcagc gcctgggtca tcacttccgg
     3181 accaataccg tcccccggca atacggcaat atggtaattc ttcgacatca cacggtttcc
     3241 ttgttgtttt cgttgtgttg agctttgcgt tgcaactctt tttcgacttc tgcggcacgc
     3301 cagatattgt tcagtacgtg caccatggct ttggcggagg actcgacaat atcggtggcc
     3361 aggccgacgc cgtggaagcg gcgaccgttg tagttggcga caatatccac ctgacccagc
     3421 gcatctttac cgtgaccttt ggcggtcagg ctgtatttca ccagttcgac gttatagtca
     3481 gtgatgcggt taattgcctg atagacggca tcgaccggac cgttaccgtt ggcggcttct
     3541 gctttgactt cttcgccaca ggccagtttg acggcggcgg tggcgatatc gttagagcca
     3601 gactgcacgc tgaagtaatc cagacggaaa tgctccggct cttcttgctg cttaccgatg
     3661 aaggccagcg cctccagatc gtaatcaaac acctgacctt ttttgtccgc cagcttcagg
     3721 aaagcatcgt acaaattgtc taaattatat tcactttctt tataccccat ctcatccatg
     3781 cgatgtttca ccgccgcacg ccccgaacga gaggtcagat tcagctggat ttggttcaga
     3841 ccaatagatt ctggtgtcat gatttcgtag ttttcgcggt ttttcagcac gccatcctgg
     3901 tggataccgg aggagtgagc gaatgcgctg ctgccaacaa tggctttgtt tgccgggatc
     3961 ggcatattac aaatctggct aactaactgg ctggtgcgcc atatctcctg gtgattaatg
     4021 gcggtgtgga cgttgagaat atccttacga actttaatcg ccatgatgac ttcttccagc
     4081 gaacagttac cggcacgctc gccgatcccg ttcattgcac cttccacctg gcgtgcaccg
     4141 gcatgtaccg ccgccagtga gttgccgacc gccaggccta aatcgtcgtg ggtatgtacg
     4201 gaaataatgg ctttgtcgat gttaggtacg cgttcataca ggccgctgat gattccggcg
     4261 aactcaagcg gcatggtgta gcccacggtg tccggaatgt tgatggtggt ggcaccggca
     4321 ttaatcgccg cttcgaccac tcgcgccaga tcggcaatgg gtgtacgccc ggcatcttcg
     4381 caagaaaatt caacatcatc ggtgtaatta cgggcgcgtt tcaccatata gatagcgcgt
     4441 tcgatcacct cgtccagggt gctgcgcagc ttggtggcga tgtgcattgg ggaggtagca
     4501 ataaaggtat gaatacggaa ggcttcggcg actttcaggg attcggccgc cacgtcgata
     4561 tctttttcca cgcagcgagc taacgcacat acgcggctgt ttttaacctg gcgggcgatg
     4621 gtttgcaccg attcaaaatc gcccggcgaa gagacgggga aaccgacttc catcacgtca
     4681 acacccatac gctcaagggc cagcgcaatt tgcagttttt ctttcacact caagcttgcc
     4741 tgtaacgcct gttcaccgtc gcgcaatgtg gtatcgaaaa taatgacttg ctggctcatg
     4801 gtttaggtcc ttgtctcttt tagagcgcct cgcttcgggc ataaaaaaac ccgcgcaatg
     4861 gcgcgggttt tttgtttgac tgcgtgctgg cttaatgctg gatgccgctc actcgtctac
     4921 cgcgcaaaga agatgcgttt agtagtagta gaccgataaa gcgaacgatg tgagtcatta
     4981 aatcagctcc agatgaatgc gatatgcttt tagagttact ggatacaaaa acggatgtca
     5041 accctgacgc aataaaaacg tcccgccagc gtgagttctg catccgtaaa attagctaat
     5101 tgtgctgcgg tggttaaagt aagcgatatt aatttctgct taactaccga cgcttttcat
     5161 cggttgacat atttcagcat aaatttttgc atctaatcaa cgaggaaaaa ggggacaaaa
     5221 tgcacgcgtt gcaaaaccta
//
LOCUS       DAZRUF010000250         5237 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZRUF010000250 DAZRUF010000000
VERSION     DAZRUF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.252"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHXOL_003706"
     CDS             complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHXOL_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR6810580.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IV"
     gene            1022..1552
                     /gene="cybB"
                     /locus_tag="ACHXOL_003707"
     CDS             1022..1552
                     /gene="cybB"
                     /locus_tag="ACHXOL_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR6810581.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1797..1970
                     /locus_tag="ACHXOL_003708"
     CDS             1797..1970
                     /locus_tag="ACHXOL_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810582.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            1996..2064
                     /gene="yncP"
                     /locus_tag="ACHXOL_003709"
     CDS             1996..2064
                     /gene="yncP"
                     /locus_tag="ACHXOL_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR6810583.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHXOL_003710"
     CDS             complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHXOL_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR6810584.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2590..4230
                     /gene="trg"
                     /locus_tag="ACHXOL_003711"
     CDS             2590..4230
                     /gene="trg"
                     /locus_tag="ACHXOL_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR6810585.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(4268..5191)
                     /locus_tag="ACHXOL_003712"
     CDS             complement(4268..5191)
                     /locus_tag="ACHXOL_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR6810586.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
BASE COUNT         1354 a         1214 c         1319 g         1350 t
ORIGIN      
        1 actatttctt catcggtata accaaatgac tcgttattgg tggttgcttg tttaagtgcg
       61 ttattcacct cttcggcagt cacttttttt ccgagaatcg ataccagttc agtgaccgaa
      121 cctgttttca ccggcacgcg ttgcgcatga cctttcagtt tgccgctcag ttccgggatc
      181 accagaccaa tggcttttgc cgcccccgta gtgtggggaa tgatattttc tgccgctgcg
      241 cgtgaagcac gtaaatcttt accacgtggg ccatccacca gtgactgggt gccagtatag
      301 gcatgaatgg tcgtcatcgt gccgacttct atcccgaaac tgtcatgcaa ggctttggcc
      361 atcggcgcaa gacagttagt ggtgcatgac gccacggaaa caatggtgtc gttgccatcc
      421 agagtgtcgt cattgacgtt ataaacgata gttttcattt caccggcagg ggcggaaatc
      481 aacaccttct tcgcaccagc atcaagatgt gcctgcgatt tctcggcgga ggtataaaag
      541 ccagtacatt cgacaatgat ttctgcacct ttcgctttcc acggaatatt tttagcctct
      601 ttttcggcgt aaaccgcgat acttttccca tcaacgataa gtgaatcttc cgtaaaatca
      661 acgctccagg ggaatggtcc gtagtttgaa tcatgtttca gcaggtaggc gagaattttt
      721 ggggaagtga gatcattaat agcgacaacg tctatgttgc ttttgacttc aagtaatcga
      781 cgcaacacca gtcgaccgat acgaccaaaa ccgttaatac caactttact catggttttc
      841 tcctgtcagg aacattcgga tgaaaattga tcctttccag gcttagacca ggattgtggg
      901 atgggcaatc tccattctta cagtgaaacg taacgtactg aaaacgggtg aacaatattt
      961 aatgaaattt tgagaaaagc ccgttatgtt aacggaaaat tatgttaaag caggaaatgt
     1021 tatggaaaat aaatactcaa ggttacaaat cagcattcac tggctggtct ttttgctggt
     1081 tatcgcagcg tattgcgcaa tggagtttcg tggtttcttc ccacgtagcg atcggccact
     1141 catcaacatg attcatgttt cctgcggcat ctcaattctc gtgctgatgg tcgttcgtct
     1201 tttgttaagg ctgaaatacc caaccccgcc gattatacct aagccaaaac cgatgatgac
     1261 gggactggcg catttgggac atttggtgat gtatctcctg tttattgcgc tgccagtgat
     1321 tggtttggtg atgatgtata accggggcaa cccgtggttt gcgtttggtt tgacgatgcc
     1381 ttacgcttca gaggccaatt tcgaacgggt tgatagctta aagtcgtggc atgagacgct
     1441 ggcgaatctg ggatattttg tcatcgggtt gcacgctgcg gctgcactgg cacaccacta
     1501 tttctggaag gacaacacac ttctacgcat gatgccgcgt aaacgttcct gaaggatatt
     1561 tcaagaaaat gcctgtacta aaaccgaccc gtggtacagg cgaagaatac gggtctacat
     1621 cggaagcgcc tattatattt atttgtatga caaataaaac cataatcctt gcccatacgt
     1681 ccatctggct tatttttaat caattcaccc gatctttgat ctcatcaacg gtatcaaaat
     1741 aaaccagcgt ataacgttca aatcgaacat aagattcgat aaaccatgga ggttatatga
     1801 aaaaactggc acttattttg tttatgggaa cgcttgtttc cttttatgcc gatgccgggc
     1861 gcaaaccctg ttctggttcg aaagggggga tctcacactg tacggcaggc ggcaaatttg
     1921 tctgtaatga tggttctatt agtgcatcga aaaaaacatg cactaactga agtgtaaaag
     1981 gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg ggaaaatccc cgcgcttgcc
     2041 cttacctgga cgtgcaggcc atgagcgcag caacctcctt atcaccgtcc cggaaccgca
     2101 gttcgtagag cgtttgtcgg gtcaggagtg tgaatgtcag aatcgtaatg cagataatga
     2161 gcagacacac taccagaggg ttgtgcttca tagcctttct ccttgccgga tggcgggtaa
     2221 gaggctaaga tctgaattgc taggttcatt cgttggcctc ggttgataga aatatcggtc
     2281 ggggccttcg tctttctgat tcccggttag cctgaaaaca gaaagtctca ggcacccgca
     2341 ggcatcctat gaggtttcct tagggacgaa aataatcact tcacgaaatt gcgtgctgtt
     2401 ttccagaatt tttcgtcatt cgggttagcc agtttagcca ttcgttactc tcttcattcc
     2461 aatagcatta attttctctg caataattgt tgtaaaaatg tgacgcaaag aggtttttgg
     2521 tcataagtaa ttaccgtcaa gtgccgatga ctttctatca ggagtaaacc tggacgagag
     2581 acaacggtaa tgaatacaac tccctcacag cgattaggtt ttttgcatca catcaggctg
     2641 gttccgttat ttgcctgcat tcttggcggt atcttagttc tattcgcatt aagttctgct
     2701 ctggctggct atttcctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt
     2761 gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg
     2821 attcaggccg gggctgcgag ttgtattgcg gaaatggaag caatgaagcg aaatattgcg
     2881 caagccgaat cggagattaa acagtcgcag caagggtatc gtgcttatca gaatcggccg
     2941 gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt tcaggcttat
     3001 atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc
     3061 aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgaacaaa
     3121 gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc
     3181 cgcttgggtg ggatgttcat gattggcgcg tttgtgcttg ccctggtcat gacgctgata
     3241 acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt
     3301 gaaaaaatcg ctagtggcga tctgacgatg aaggatgaac cggcgggtcg taatgaaatc
     3361 ggtcgcttaa atcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact
     3421 gttcgacagg gtgcggaaga gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg
     3481 gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc cgccagcatg
     3541 gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg
     3601 gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggtttccgg tgtagtaaaa
     3661 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     3721 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     3781 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc
     3841 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     3901 ctggggtcgg atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc
     3961 gcgagtgtca cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc
     4021 ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct
     4081 ctggtagaag aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact
     4141 gaggcggtgg atgtattccg tctgcacaaa cattctgtgt cggcagaacc tcgcggagcg
     4201 ggtgaaccag ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct
     4261 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     4321 agaacgtcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa
     4381 caggcagggc caccagcgtg gcatgacgca ggtcgtcttt tacagcgcca gaagggacaa
     4441 accacacgta atcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     4501 tacaacccga aggaatttta cagccctggc tctgcactaa tgcatctgaa tgctggcgtg
     4561 gcgcagtgcc ttctggtgat acaacgaccg gccattccag cacccggctt agcgttacgt
     4621 tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca
     4681 gttcgtaatt aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     4741 tttccccggt ttttaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg
     4801 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     4861 cagtaggtag tgcaccaacc ctgacgacat cattattaag accttcttta cgatgaagcg
     4921 actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc
     4981 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     5041 gctccagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     5101 cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga
     5161 tgcgctgact aaacagacta tttttttcca taagcgatgt taaaaacgaa gcggtgtcgc
     5221 tgacaagtga agttgtt
//
LOCUS       DAZRUF010000251         5227 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZRUF010000251 DAZRUF010000000
VERSION     DAZRUF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.253"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..217
                     /locus_tag="ACHXOL_003713"
     CDS             <1..217
                     /locus_tag="ACHXOL_003713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810587.1"
                     /translation="NESDEHKGNQYSRWISVVLGELLIKIWNCDTVQIQTHKKTSSLA
                     LERFLADLITINYEAQKNNISSFSTVK"
     gene            224..2929
                     /gene="malT"
                     /locus_tag="ACHXOL_003714"
     CDS             224..2929
                     /gene="malT"
                     /locus_tag="ACHXOL_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR6810588.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(2972..3988)
                     /gene="rtcA"
                     /locus_tag="ACHXOL_003715"
     CDS             complement(2972..3988)
                     /gene="rtcA"
                     /locus_tag="ACHXOL_003715"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR6810589.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(3992..5209)
                     /gene="rtcB"
                     /locus_tag="ACHXOL_003716"
     CDS             complement(3992..5209)
                     /gene="rtcB"
                     /locus_tag="ACHXOL_003716"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR6810590.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRDLAY
                     FMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINC
                     HHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSH
                     GAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVE
                     VIYTLRQVVCVKG"
BASE COUNT         1234 a         1479 c         1314 g         1200 t
ORIGIN      
        1 aaatgaatct gatgaacata agggaaacca gtattcacgc tggatcagcg tcgttttagg
       61 tgagttgtta ataaagattt ggaattgtga cacagtgcaa attcagacac ataaaaaaac
      121 gtcatcgctt gcattagaaa ggtttctggc cgaccttata accattaatt acgaagcgca
      181 aaaaaataat atttcctcat tttccacagt gaagtgatta actatgctga ttccgtcaaa
      241 actaagtcgt ccggttcgac tcgaccatac cgtggttcgt gagcgcctgc tggctaaact
      301 ttccggcgcg aacaacttcc ggctggcgct gatcacgagt cctgcgggct acggaaagac
      361 caccctcatt tcccagtggg cggcaggcaa aaacgatatc ggctggtact cgctggatga
      421 aggtgataac cagcaagagc gtttcgccag ctatctcatt gccgccgtgc agcaggcaac
      481 caacggtcac tgtgcgatat gtgagacgat ggcgcaaaaa cggcaatatg ccagcctgac
      541 gtcactcttc gcccagcttt tcattgagct ggcggaatgg catagcccac tttatctggt
      601 catcgatgac tatcatctga tcactaatcc agtgatccac gagtcaatgc gcttctttat
      661 tcgccatcaa ccagaaaatc tcaccctggt ggtgttgtca cgcaaccttc cgcaactggg
      721 cattgccaat ctgcgtgttc gtgaccaact gctggaaatt ggcagtcagc aactggcatt
      781 tacccatcag gaagcgaagc agttttttga ttgccgtctg tcatcgccga ttgaagccgc
      841 agaaagcagt tggatttgcg atgacgtttc cggttgggcg acggcactac agctaatcgc
      901 cctctccgcc cggcagaata cccactcagc ccataagtcg gcacgccgcc tggcgggaat
      961 caatgccagc catctttcgg attatctggt cgatgaggtt ttggataacg tcgatctcgc
     1021 aacgcgccat tttctgttga aaagcgccat tttgcgctca atgaacgatg ccctcatcac
     1081 ccgtgtgact ggcgaagaaa acgggcaaat gcgtctcgaa gagattgagc gtcaggggct
     1141 gtttttacag cggatggatg ataccggcga gtggttctgc tatcacccgc tgtttggcaa
     1201 cttcctgcgc cagcgctgcc agtgggaact ggcggcggag ctgccggaaa tccaccgtgc
     1261 cgccgcagaa agctggatgg cccagggatt tcccagcgaa gcgattcatc atgcgctggc
     1321 ggcaggcgat gcgctgatgc tgcgcgatat tctgcttaat cacgcctgga gtttgttcaa
     1381 ccatagcgaa ctgtcgctgc tggaagagtc gcttaaggcc ctgccgtggg acagtttgct
     1441 ggaaaatccg cagttagtgt tattgcaggc gtggctaatg caaagccaac atcgctatgg
     1501 cgaagttaac accctgctag cccgtgctga acatgaaatc aaggacatca gagaaggcac
     1561 catgcacgca gaatttaacg ctctgcgcgc ccaggtggcg attaacgatg gtaatccgga
     1621 tgaagcggaa cggctggcaa aactggcact ggaagagctg ccaccgggct ggttctatag
     1681 ccgcattgta gcaacctcgg tgctgggtga agtgctgcac tgcaaaggcg aattgacccg
     1741 ctcactggcg ctaatgcagc aaaccgaaca gatggcacgc cagcacgacg tctggcacta
     1801 cgccttatgg agtttaatcc agcaaagtga aattctgttt gcccaagggt tcctgcaaac
     1861 cgcgtgggaa acacaggaaa aaacgttcca gctgatcaac gagcagcatc tggaacagct
     1921 gccgatgcat gagtttctgg tgcgcatccg agcacaactg ttatgggcct gggcacggct
     1981 ggatgaagcc gaagcgtcgg cgcgtagcgg gattgaagtc ttgtcgtctt atcagccaca
     2041 gcaacagctt cagtgcctgg caatgttgat tcaatgctcg ctggcccgtg gtgatttaga
     2101 taacgcccgt agccagctga accgtctgga aaacctgctg gggaatggca aatatcacag
     2161 cgactggatc tctaacgcca acaaagtccg ggtgatttac tggcaaatga ccggcgataa
     2221 agccgccgct gccaactggt tgcgtcatac ggctaaaccg gagtttgcga acaaccactt
     2281 cctgcaaggt caatggcgca acattgcccg cgcacaaatc ttgctgggcg agtttgaacc
     2341 tgcggaaatt gttctcgaag aactcaatga aaatgcccgc agtctgcggt tgatgagcga
     2401 tctcaaccgt aacctgttgc tgcttaatca actgtactgg caggccggac gtaaaagtga
     2461 cgcccagcgc gtgttgctgg acgcattaaa actggcgaat cgcaccggat ttatcagtca
     2521 ttttgtcatc gaaggcgaag cgatggcgca acaactgcgc cagctgattc aactcaatac
     2581 gctgccggaa ctggaacagc atcgcgcgca gcgtattctg cgagaaatca atcaacatca
     2641 tcggcataaa ttcgcccatt tcgatgagaa tttcgttgaa cgtctgctga atcatccgga
     2701 agtgccagaa ctgatccgca ccagcccgtt gacccaacgt gaatggcagg tcctggggct
     2761 gatctactcc ggttacagca atgagcaaat tgcgggcgag ctggaagtgg cggcaaccac
     2821 catcaagacg catatccgca atctgtacca aaaactcggc gtcgcccacc gtcaggccgc
     2881 ggtacaacac gcgcagaaac tgctgaagat gatgggatat ggggtgtgag tttagccgga
     2941 taatgcattg gatccggttt acatctttac atcattcaat actcacccgc gttacgccat
     3001 ctgtttctat caaaccaaac cgcaccggca agaaacgctc caccacggcg atattggtca
     3061 gcagatggca tgaaggatgg gcgaccgtaa attctcccgc gcccgccagc gccatcggta
     3121 gcaccagctg gtcggcgaga tattccccca ctaccgccgg gcttgccagg tagcgtttca
     3181 cctctttcac taactgcgcg gctaccacct cggcactgac gcgcttttca ccgacgacga
     3241 aaaagcgttc agtgatattt tcactttcga cttccagcga gacggtatta cccggcccct
     3301 ggtcgcgcgg caggttatga atattctgtt catgcaggga aaaactcccc gccagtgtag
     3361 cgatttcacg ctcagcaaca tggcgcggca caccagctaa tagaacttct ccacgcatct
     3421 gcacaatgtt cccgcgctcg ccaagttgca aggtgttaaa cgatgccacg ggcgagactt
     3481 ccgttgccac cacgccgcct ccggcaggat aaaaaccgtg acgtaacagc gtggtttgct
     3541 gatgaattcc tattttcgcc agcagcggct ccagcacccg gcggataaaa tccgcaggcg
     3601 gggccgacgg gttatcggtg cctccgctca cttcaacacg cgaaggtcca tcggcaaacc
     3661 acagcgcggg cagcaccgtt tgcagcacca gcgtacagct tccggcacta ccgatagcaa
     3721 agcggtactc gccgccgcgc acggtgccaa gccggaagac cagacgctgc gaccccagct
     3781 ccgcgccttc caccgttgcc ctacaaattt ccgcagccgc ttttaccgcg gtcagatgct
     3841 ggcgcaacag ccccggtttc gcccgcccgg cacgaatgct ggtgatggta aatggctggc
     3901 cggttatcat cgacaggctc agcgccgagc gcaggatctg cccgccacct tcgccctgtg
     3961 cgccatccag cgcaatcatc cttttcatca tttatccttt tacgcacacc acctgacgca
     4021 gggtatagat aacttccacc agatcgcttt gtgccgccat caccgcatca atatctttat
     4081 acgccatcgg gatttcgtcg atcacttcgg catctttacg gcattccaca tgcgcggtgg
     4141 cacgaatttg atcttccacg ctgaacagtt ttttcgcttt agtacggctc attacccgcc
     4201 cggcaccgtg gctgcacgaa cagaaagatt cttcatttcc cagcccacgg acgataaagc
     4261 tttttgctcc catcgaccct ggaataattc catattgacc agcacgcgca aacaccgcgc
     4321 ctttacgcgt cacgtagatc tcttcaccaa agtgctgttc tttttgcaca tagttgtggt
     4381 gacagttgat ctcttccatc gccagcgttt gtggctgtct gaccgttttc tgcgtaatgc
     4441 tctgcaatgc cgttaccacg ttttccatca tcgcatcgcg gttaaggctg gcgaaaagct
     4501 gcgcccaggc cacggctctt aggtaatcat caaagtattc cgtaccttcc ataaagtacg
     4561 ccagatcacg cgacggcaac gtctcaagtt ggtcctgcat ctctttttgc gccagatcga
     4621 taaagtaagt cccgatggcg ttaccaattc cgcgtgaacc ggagtgcagc ataatccaca
     4681 cctgctccga ctcatcaagg cagatttcaa taaagtggtt acccgttccc aggtgtttat
     4741 agttgttagt gttaaggaaa cgcggatatt tttgcgttaa ccactgataa ccggcctcca
     4801 gctcagccca tttcgcatcg acgttaaaag gcgggttttc ccatgcgcct ttatcacgtt
     4861 tacaacgccc ggtagtacgc ccgtgtggta cggccgtttc aatcgcttga cgcagctccg
     4921 ccaggttttc aggcaggtct gccgccgtta acgcggtacg cagcgcgttc attccacagc
     4981 caatatccac gcccaccgcc gccggaataa tcgccccttt ggtcgggatc acgctaccaa
     5041 tggtggaacc tttacccagg tgtacatcag gcattaccgc aatatgtttg aaaataaacg
     5101 gcatcttcgc cgtattaata agttgctgac gcgcatcggc ttctaccggc acgcctttgg
     5161 tccacatttt taccggggca ttttcagtgg tcagtaattc gtaattcatt ttgtttttct
     5221 cttttcg
//
LOCUS       DAZRUF010000252         5197 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZRUF010000252 DAZRUF010000000
VERSION     DAZRUF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.254"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(36..794)
                     /gene="aroD"
                     /locus_tag="ACHXOL_003717"
     CDS             complement(36..794)
                     /gene="aroD"
                     /locus_tag="ACHXOL_003717"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR6810591.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(825..1691)
                     /gene="ydiB"
                     /locus_tag="ACHXOL_003718"
     CDS             complement(825..1691)
                     /gene="ydiB"
                     /locus_tag="ACHXOL_003718"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR6810592.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1703..2968)
                     /locus_tag="ACHXOL_003719"
     CDS             complement(1703..2968)
                     /locus_tag="ACHXOL_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6810593.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3195..4409)
                     /locus_tag="ACHXOL_003720"
     CDS             complement(3195..4409)
                     /locus_tag="ACHXOL_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6810594.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4509..4865)
                     /locus_tag="ACHXOL_003721"
     CDS             complement(4509..4865)
                     /locus_tag="ACHXOL_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR6810595.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5001..5108)
                     /gene="rprA"
                     /locus_tag="ACHXOL_003722"
     ncRNA           complement(5001..5108)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHXOL_003722"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1519 a         1191 c         1181 g         1306 t
ORIGIN      
        1 cggcggggag ggtgttcccg ccgaaatatt attgcttatg cctggtgtaa aatagttaat
       61 accgtgcgta aatcatttac cgagatttgc cctggtgcag aggctttttt taccgcacca
      121 aaagttgccg ccgagccaaa tacttcacca gccagacgag aaattacgcc agtttttgcc
      181 atcgacatcg tgatgattgg acgatcggca tactgctcct gcatctccag ggtcgcggta
      241 agcaacgtca gcacatcgct ggtactttgc ggcatcagcg caatcttagg aatatcggcg
      301 tcgaaggact gcattttgcg cagacgggca atgatttctt cggcttccgg cgttttatgg
      361 aagtcatggt tggacatgac aactttcaca tcatgcgcgt gggcgtaggc gacggtttct
      421 ttaacctgat catcgccggt aaataactcc agatcgatca tatcaaccag gccgctgtcg
      481 atggctgcac gattgagagc aatataagcc tcggtggaaa tcgcctgctc gccgccttct
      541 ttggcactgc ggaaggtaaa cagcagcggt ttttctggca tggtttcacg gagaattttt
      601 gccgccgcca tgacagactc cacattggag aggtcggcaa agtggtccac acgccattcc
      661 agaatatcaa agtccgcttc acgataggcg agagcttcgg atttcacgcg ggcgatatct
      721 ttcgccatca gcgagacgat gattttaggt gcgcccgcac caatgacgag atcttttaca
      781 gttacggttt tcatttttta ccctttctgc acgcggtcag cctgtcaggc accgaacccc
      841 atgacctgtt taacatattc cagagggaaa tctttgccag tccataacgt gaactgttca
      901 gccccttgcc acaacaacat gccgtatcca tcaatcgttt tgcaacctgc ttgttgtgcc
      961 tgctgcaata acttcgtcat atgcgggtta tacacgcatt cagtgaccag cagtcccgga
     1021 tgtaacagac tgatatcatt aaccaatgat tcattctcaa gggggttcat acctactttt
     1081 gtgccattgg ttaaaatgtc ggcggaagcc aggacttcag caaaggcttg ctgatcggcg
     1141 agatcggtaa ccgtgacgac acaatcggtg ttttcgttaa cccgctgcgc gaaggcgagg
     1201 gctttatcga agaactcatc ccgacggtta aagagtttaa tttcttttaa accttcaatt
     1261 gccccctgcg cgccaatcgc tgttgaggca cctccggccc ctaacagcac catcgttttg
     1321 cctttgatat caaaaccgct ctctttaatg gcgcgaatat ggcccgtgcc gtcggtgtta
     1381 tagccacgca gatagccatc atcattaacg atggtgttga tggcacccac cagtttggct
     1441 gctggcgtta attcatcaac atattcacac gccagttgtt tgttcggcat cgacacacca
     1501 gttccgcgca ttttgagggc ttttaatcct tcaattgctc cgggaaagct atcgttatcc
     1561 acttcgaagg ccatataggt aaatggcaat cccgcttttt ctaaggcttt attctgcatt
     1621 tcaggcgata aactgtggcg gataggataa gccatcaacc caatcaattc gtattttgcg
     1681 gtaacatcca taattaactc ctttaacctc tatgcttaat tgtgtttaac cgggtgctaa
     1741 atttgcgttc gccaaaccgc acatcattct caggaataat aaaaacgcgg taatagcggc
     1801 taaaaacaat aattgcggta atcagggcca gcagcgcgaa agtaaaatcg agaacaatga
     1861 tatattgcag gccgatgttc gacagataac cggtaatcag cggaataaca aagttagcca
     1921 gtccacccat catcatataa atactggtga ctttggcttt gcttttgggg aaaaactctg
     1981 acatgaccga aacaccgagc tgtaaaatgc cgccagctgc tgagaaaccg ataacaaagg
     2041 cgccggcatt acacaccagt ggagaagggt acaggtagat aatggcagct gttattgttg
     2101 ccagcgcaga gttaaataca ttagcccaga tgggccggac catttttttc agtagtgcgg
     2161 caaaaataaa gacacagacc aacgagccca tactgtaata agagatggtt tttaatgcct
     2221 cagcttctga cataccagca aaagccatcg catatttggg catccacacc acaatcacat
     2281 aaaaggtcga gaatgcggct acaccgaaca gtaccgatga aacaccttcc agccagacta
     2341 acggtttgct gttcatttgc ggtaattcat tagctacgct ggcgtccacc aactggctgg
     2401 ggaatttgct tttcaacagc atcagcgtga tcagtacaaa cagaataccc ggaataatca
     2461 gcccatagcc gtaccagata ttattgagca acatatagct caccagcatt gggtagaaca
     2521 tttgcccaaa tgacaccatc gctttaacca gtatgaccgc cgaaccagag gctttcggaa
     2581 agcattccat gagcgcgggg tagccacccg tatccagcgc tgagttagcg atacctacgc
     2641 acactgccag accgtaggcg agagttaaat tcgggcaagc gggaatacca aagaagaata
     2701 gcagatacat tattactgcc attaatatca ccgcccgacg accaaactta tcggagatca
     2761 caccgaagaa taaaatactg atcaatcgcc ccaaaccgat accggaaatt aaataggcaa
     2821 tgcccgcgtt gtcagtggaa aacttttctg ccagagatgt catattttgg gcaagcgtaa
     2881 taacactaat gccgtgcagg aaatagctga agtaaataca aagaacagcc aggataaatg
     2941 gcgtgctgaa agccttattt tgagacattt tcacagctcc ttgctgaaag ataatttcgt
     3001 taccgtacgt attgttctcc gacggtttac ataattacta tactgaagat gattttccct
     3061 gggtacatgg cataaagttt atgtttccag gaatattgca tgtcatagtt tgtttttgat
     3121 cgcgattttc cttagttagc gcagaaagag ttatttatcc gtaagatctt gcatgacctg
     3181 aaaagaatat tgcattaccc acccggagcg acattttcct ttagcgagtg atgccgcgtt
     3241 tttttgcggc tgcgtaagcc gataaacagt gccagtaaaa aaccgatggc agcgatggcg
     3301 gtatcgaacc acataatatc ggcaatactt ctttgggaca gatgagccgt aatcaacgga
     3361 atagtaaagg tcgcaatact gcccgcactg taatagatcc ctgtagcttt acctttagcg
     3421 taagggaaac gttcagccat taacgtcagg ccaatttgca caacacctcc agcagaggta
     3481 aaaccaatga caaaagcaaa tattatcacc acataaaatg tgggatgcag gcagacggta
     3541 aacagagcaa taaatgagat aaaggtgtac agcatcagta atgttgttgg gcgaacggta
     3601 ttacgaatga gtggagcggt aataaataca caaagcagcg aacccacggt gtagatactg
     3661 agtagtttga tcgacatagt gtatgacatg cctgcaacaa attgtccgta ctgtgccagc
     3721 cactggctaa ccagataaaa cgttgccatt gagatatagc catataaggt ataactggct
     3781 aaatcgataa ttgaacagcg atgttccgta gagctggtgg ttttctttat gacaggtaag
     3841 cgacgacccg gatggggtgg gaacgtacaa cggtataaaa acagagcgtt aataaacata
     3901 atgcctgcag caatcataaa ggaccaaccg aaccacagtt cagcccacac taacaggcta
     3961 atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg ctttaattaa aatattggct
     4021 gtcccaggtg agcgtggaaa agcttccatc aaactgggat aagtgcctgc atcgagaaaa
     4081 ctgtttgcca ttcccgccag aaagccaaaa acataagcga taatgatgtt attggtctgc
     4141 aggatgccaa aaaagaaggc catatagcag cacatcccga gcatgataaa agggcggcga
     4201 ccaaagcgat cggataataa tcctgcaaaa agcaagacac ttaatcgacc aatgcccagc
     4261 gatgagataa ctatcgagac acccgcggca ttagtctgcc aaagtgtctc cagcgaggcc
     4321 atattcaggc tcatcaaaat gacgcccata ccatgcacca ggtaattaaa atacaaccca
     4381 agtgcggtag ggtaataggg atttttcata ggttctactt agcaaaggtg ctttaaaaag
     4441 tgtagcgatg ttgtgtcagt atccattcat tattgttgta acaacgacaa ttcctttctg
     4501 tggaaaaatt aacaaagccg ctccagatcg tgtgcatata attcagcggc taccgattga
     4561 tagatcttcc agtcaataaa attgccatca gggtagactt gttgaaacgc ggttaagtcg
     4621 gggaaaaaac gcatggtgtt gttgccgatg cggttattta ttttctctat gatggcatga
     4681 agatgttttt ttcgttgctg acgcatagcc aacagttgct cgactacaca atcaggaatg
     4741 gcgcatttgc cattttccca ctgacgccag ctttcactgt caccggtctg ggcaatccag
     4801 gtcgcgcatt catcaatagt catggcaaaa atatggcgga gtgcctggag ttcataagcg
     4861 ttcatcgtgt aatggctgca tgaaataaac aataaaatat aaataacatt atcagcctgc
     4921 tgacggcttg aagagagtca cagtatcttg tgcaacatta ttggaaagag tgaggggcga
     4981 ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg ggagatgggc aaagactaca
     5041 cacagcaatt cgttgtttca ctcaggggat ttccatgctt ataaatcaat atgttgattt
     5101 ataaccgtga gctaatagta ggcatacgga ctttttgcgt cgatcagatt cgtctcatta
     5161 gtttaaaaac tgtaaagaat ttgcgacagg tactacc
//
LOCUS       DAZRUF010000253         5177 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZRUF010000253 DAZRUF010000000
VERSION     DAZRUF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.255"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(563..939)
                     /gene="rnpB"
                     /locus_tag="ACHXOL_003723"
     ncRNA           complement(563..939)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHXOL_003723"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(972..2117)
                     /gene="garK"
                     /locus_tag="ACHXOL_003724"
     CDS             complement(972..2117)
                     /gene="garK"
                     /locus_tag="ACHXOL_003724"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR6810596.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2214..3104)
                     /gene="garR"
                     /locus_tag="ACHXOL_003725"
     CDS             complement(2214..3104)
                     /gene="garR"
                     /locus_tag="ACHXOL_003725"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR6810597.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3134..3904)
                     /gene="garL"
                     /locus_tag="ACHXOL_003726"
     CDS             complement(3134..3904)
                     /gene="garL"
                     /locus_tag="ACHXOL_003726"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR6810598.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3920..>5177)
                     /gene="garP"
                     /locus_tag="ACHXOL_003727"
     CDS             complement(3920..>5177)
                     /gene="garP"
                     /locus_tag="ACHXOL_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR6810599.1"
                     /translation="TAVNYADRATLSIAGTEVAKELQLSAVSMGYIFSAFGWAYLLMQ
                     IPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFSEAP
                     SFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTVMGG
                     IGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLHYIK
                     QLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFAG
                     GVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLVSTIILCNYTNNTTLVVMLMALAFFG
                     KGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVF
                     VGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1248 a         1506 c         1234 g         1189 t
ORIGIN      
        1 tcttaccctg tgcaccaaaa gcaaaaactc actgacgcgg gtttgaaact gatggcaacc
       61 gcaaaaatgc ctgatgcgct acgcttatca ggcctacgcc atctctacaa tatattgaat
      121 ttgcgtgctt ttgtaggcag gataaggcgt tcacgcagca tccggcatga ataaagcgca
      181 ctcaacaatc tgaaacccgc cggaacggtt tattacgtac atcaggtgaa actgaccgat
      241 aagccgcttt cttttgggta tagtgtcgtg gacagtcatt catctttctg cccctccaaa
      301 agcaaaaacc cgccgaagcg ggtttttacg taaatcaggt gaaactgacc gataagccgc
      361 tttcttttgg gtatagcgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa
      421 acccgccgaa gcggtttttt acgtaaacca ggtgaaactg accgataagc cgctttcttt
      481 tgggtatagc gtcgtggaca gtcattcatc tttccgctcc tccaaaagca aaaacccgcc
      541 gaagcgggtt tttacgtaaa tcaggtgaaa ctgaccgata agccgggttc tgtcgtggac
      601 agtcattcat ctaggccagc aatcgctcac tggctcaagc agcctacccg ggttcagtac
      661 gggccgtacc ttatgaaccc ctatttggcc ttgctccggg tggagtttac cgtgccacgg
      721 actgttacca gccgcgcggt gcgctcttac cgcacccttt cacccttacc tgatcccgct
      781 tgcgcgggcc atcggcggtt tgctctctgt tgcactggtc gtgggtttcc cccccaggcg
      841 ttacctggca ccctgcccta tggagcccgg actttcctcc cctccgcccg tctcccccga
      901 agaggacgac gacgaagcgg cgactgtctg gtcagcttcg gcgcgcagta tagagggttt
      961 gcgcgccctt gtcaccccgc gttgcgcatt ccaatcgcca gtgtcgcggc gatattacgt
     1021 gaagcacggc agatattgtc ataagccccg cggaatgctt cgtccaacgt acctatgctg
     1081 gtcaatacgc tgaagaccgc atcgatgcca tgctgatgta caacgccaac atcatcggtc
     1141 aggctacccg caatgccaat caccggttta tggtacttct tcgccacgtt tgcaacgcca
     1201 atcggtacct tgccgtgaat actctggctg tcaatacgcc cttcaccggt gatcaccagc
     1261 gtacaatcgt gaatatgttc ttccagattc agcgccgtag tgacgatttc aataccactt
     1321 ttcagttccg caccaagaaa cgccatcagc gccgcgccca taccgcccgc agctcctgcg
     1381 ccggggacat ctttcacatc aacatgcagc gcttttttaa tgacatcggc atagtgagag
     1441 aggttattgt ccagctcaac aatcatcgct tcactggctc ccttttgtgg gccaaagatg
     1501 cgcgatgcgc cgttatcgcc caccagcgga ttggtgacat cacaagcgac gcgaatgacg
     1561 caatctttta agcgcggatc gaggccggaa atatcaatat cattcagagt attaagacta
     1621 ccgccgccaa aaccaatttc attgccgttg gcgtcgcata atttcgcccc cagcgcctgt
     1681 accatgcctg cgccgccatc atttgtagcg ctgccgccaa tgccgataat aatgtttgtt
     1741 gcactgctct ccagcgcctg caggattaac tcgcctgtgc cgcgtgaagt ggttacgagt
     1801 ggatcgcgtt tttccgcagg taccagctcc agcccactgg ccgccgccat ttcaataaac
     1861 gcggttttgc catcgccgga gatcccccaa ctggcattca ctttctcgcc cagcggccct
     1921 gtaacccagg cgtgacgttc agccccctgg gtggctgcaa tcatcgcttc caccgttcct
     1981 tcaccaccgt cggcaaccgg aacagaaaca tactgtgcat caggaaaaat ttcccgaaat
     2041 cctttttcta tcgcctgcgc aacctcgctg gcagataaac tttctttata agagtctggg
     2101 gcgattacga ttttcatacc tatgcctgtt accacatgac gccggagggc gtttctctta
     2161 ttcggcctag attccaggcc cggattgcaa tacaccatcc gggcacgacg tcattaacga
     2221 gtaacttcga ctttcgccag tttttcgtag tagcacgcca gggcgctatg atccgccgtt
     2281 cctaaaccat ctgcacgcag tgcctgcatc atctccataa ccgcagctgt gagcagcagt
     2341 tgtgcgccga cgccgtgaga agtatccagc gcattcgcca gatccttaat atgcagatca
     2401 atgcggaagc ccggcttgaa gttgcggtcc atcaccatcg gcgctttggc atccagcacg
     2461 gtactgcccg ccagcccacc gcgaattgcc tgataaacca ggtccgggtt aacgcccgct
     2521 ttggttgcca gcgttaacgc ttctgacatc gcagcaatat tcagcgccac aatgacctga
     2581 tttgccagtt tggtgacgtt acctgcaccg atttccccgg tatgcaccac ggaacccgcc
     2641 atcgctttca tcaaatcata gtatttgtcg aaaatagcct tgtcgccgcc caccatcact
     2701 gacagcgtac cgtcgatggc tttcggttca ccgccgctca ccggagcatc cagcatatca
     2761 atgcctttcg ctttcagcgc ttcgctgatt tcacggcttg ccagcggtgc gatagaactc
     2821 atatcgatca ataccgtacc tggcttcgcg ccttcaataa tgccattctc acccagcgcc
     2881 acctctttca catgagggga gtttggcagc atggttatga tgacgtcgca ctgttcagcg
     2941 atcgctttag ccgtagacgc tgtttctgca cctgcagcaa tcacgtcagc aatagcttct
     3001 gggttacggt cagcaaccac cagcgagtaa cctgctttca gaaggttttt actcattggt
     3061 ttacccataa tccccaggcc aataaaacca actttcatag tcatatcaac catctctctt
     3121 gttgcggtgg tggttatttt ttaaaggtat cagccagttt ctgagtggca gagcggaaga
     3181 cgccaagatc gctgccgaca gccacaaacg tcgcgcccca ttccagataa cgacgcgcat
     3241 cggcttcgac cggcgcgaga ataccgctgg gtttgccgtg cgcgctggca cggttaaaaa
     3301 tatgctgaat tgctttttgt acatccggat gcgaggcatt gccgagatgg cccaatgccg
     3361 cagccagatc gctggggccg acgaagatac cgtctacacc ttcggtagcg gcaatagcat
     3421 cgacgttatc tacgccctgc tgactttcta tctggaccag aatagtaatg ttcttgttcg
     3481 actgagcgaa ataatccgcc acggtgccaa acatattggc gcggtgagaa acggagacgc
     3541 cgcgaatgcc ttccggcggg taacgagttg atgccaccgc ctgctctgct tcctcttttg
     3601 tttctacaaa aggaatcagg aagttataga aaccgatatc cagaagacgc ttaataatta
     3661 ccggctcgtt ggtcggcact cgcactactg gcgcgctggc gctgcctttc agggccatta
     3721 actgcggaat aaacgtggaa atatcgtttg gcgcatgttc gccatccagc accagccagt
     3781 caaacccagc caaaccaaga acttcagtgc taatcgggtt agagagtgct gaccagcaac
     3841 caatttgtac ctgtttcgca gccagtgcgg ctttgaattt attcgggaaa acatcgttat
     3901 tcatcgctta tacctttgct tatttctgca attccatacg tttaatgtcg ccaactacga
     3961 agaggtagca gaccatcgcc atcagcgctg aacatcccac gaaaaccagt gctgcattga
     4021 aggagtgcag ttcacttacc aggtagccaa tcaccagtgg agtgacaatg gaggcaacat
     4081 tgccaaagac gttaaagacg ccgccacaga ggccaacaat ctctttcggc gcggtgtcag
     4141 aaatcaccgg ccagcccaga gcaccaaatc ctttgccaaa aaaagccagc gccatcagca
     4201 tgaccaccag cgtggtgttg ttggtgtagt tacataagat gatggtggaa accagcaaca
     4261 ttcccagcac aatcggtagc ttacgtgcca gggtcaggga taaaccgcgt ttgatcagat
     4321 aatccgagaa gacacctccc agcacgccgc ccgcaaaacc acacagtgcc ggaatcgagg
     4381 cgaccagacc cactttcaga atcgacatgc ctttttcctg caccagataa atcgggaacc
     4441 aggtgaggaa gaaccaggtg atggtgttga taaaatattg tccgaaaaat acgcccaaca
     4501 tcatgcggtt agagagcaat tgcttgatgt aatgcagttt gggaccgctt gctgccgcgc
     4561 tgcccggctt tttgtggtcc atatcgacca ccgcgccatt ttcagagata aacttcagct
     4621 cttccgcaga catacgtggg tgatctgtcg ggttatgaat caacttgatc cacagcgccg
     4681 tcagcacaaa accaatcccc cccataacgg tgaagacgtg ctcccagccc caagcgaaag
     4741 tcagccagcc aagcagcggc gaaaagagcg ccagcgagaa atattgcgcc gagttaaaga
     4801 tggcggaggc agtaccacgt tctttcgtcg ggaaccaggc ggcgacaata cgggcgttcg
     4861 ccgggaatga tggcgcttcc gagaacccca gcataaagcg cataaagaac atggagatcc
     4921 ctgcccaggc cagcgggaac atatcaacaa agccttgcag gaaggtgaac agcgaccaga
     4981 aaaagaggct gtaggtgtaa acttttttcg agccaaactt atcaagcaac cagccgccgg
     5041 ggatttgcat cagcaagtag gcccagccaa aagcagagaa gatgtaaccc atcgaaaccg
     5101 cacttaactg caactctttt gccacttcgg taccagcaat agacagcgtt gcacgatcgg
     5161 cgtagttaac ggcggta
//
LOCUS       DAZRUF010000254         5172 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZRUF010000254 DAZRUF010000000
VERSION     DAZRUF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.256"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(1..1135)
                     /locus_tag="ACHXOL_003728"
                     /pseudo
     CDS             complement(1..1135)
                     /locus_tag="ACHXOL_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1135..1317)
                     /gene="ymfR"
                     /locus_tag="ACHXOL_003729"
     CDS             complement(1135..1317)
                     /gene="ymfR"
                     /locus_tag="ACHXOL_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR6810600.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1329..3062)
                     /locus_tag="ACHXOL_003730"
     CDS             complement(1329..3062)
                     /locus_tag="ACHXOL_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR6810601.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3059..3553)
                     /locus_tag="ACHXOL_003731"
     CDS             complement(3059..3553)
                     /locus_tag="ACHXOL_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR6810602.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3675..4025)
                     /locus_tag="ACHXOL_003732"
     CDS             complement(3675..4025)
                     /locus_tag="ACHXOL_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR6810603.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4068..4532)
                     /locus_tag="ACHXOL_003733"
     CDS             complement(4068..4532)
                     /locus_tag="ACHXOL_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR6810604.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4529..5026)
                     /gene="rrrD"
                     /locus_tag="ACHXOL_003734"
     CDS             complement(4529..5026)
                     /gene="rrrD"
                     /locus_tag="ACHXOL_003734"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR6810605.1"
                     /translation="MSPSLRKAGAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5026..>5172)
                     /locus_tag="ACHXOL_003735"
     CDS             complement(5026..>5172)
                     /locus_tag="ACHXOL_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR6810606.1"
                     /translation="FLQWLDQVSPSQWAAIGVLGSLLLGLLTYLTNLYFKIREDKRKA
                     ARGE"
BASE COUNT         1133 a         1516 c         1280 g         1243 t
ORIGIN      
        1 ccgggacgcg gattcatatc ttccaggtcg cggcagtcat tggaagagta aattccccag
       61 ttgatcccgg tggcgtaggc ttcaaaacgg gacttcatat ccccgcgcag taacgccccg
      121 gcgttaaatt tggcgtaata aacgccctgc ttactttttc gtaccagtcc ggtgttgatc
      181 cgctgttcga tgcgggtcag atacggcacc agtgaatagt tgataaatcc cagccccagc
      241 tcttcgatat tgttgaaggt ggcgcgatcg gtgttctgca ccatgtgcaa cggcacccgg
      301 aacagacgac agatttcttc cagctgaaac ttgcgggttt ccaggaactg actgtcctcg
      361 gcgttcagcg ccatcgactt ccagtccagc cccatttcca gaatcatcgg gcggtgagca
      421 ttgccaagcc ccgtgtgaca ctcctcaaaa tctttcttca gacgctcgta agcatccggc
      481 gtgagctttt gttccgtacg caacacaccg gatgtcaccg caccattact aaacaacctg
      541 gcaccgtgct cctcggttgc cgctgccagt gaaatggcct cacgcgcata cgcaatggga
      601 ttcagcccga caagtccatc cagcgtcagg gtgcgcacat gccagatttc attctgggtc
      661 aacacatcca cggaaccatc cggaaacgtc acctgataaa ccggctgcca ctggctgttc
      721 agcttcggtt ccacacagcc cggatctatc ggaagaagct ccaccacttc tcccagtgcc
      781 tttaccttgt aggcgtaaaa attcccccgt agacacaggc agacaataac cagctcccag
      841 aattcctgcg gtgtcatgta gccattaggt tttgccgaaa ccaacttatg cagtcgttca
      901 tccaccgccc gtgttttcag ggtaccgctg attttgtaga gactgcaggg cagcatacca
      961 acagactctg ccagcaccct gacgcaagaa tagaccgccg tcaggcgcat ggcccgctgg
     1021 ctactgatcc gctttccggt ataggtgtcg tatgacagcc cgatagcatc agcagttctg
     1081 ctggcgtggt caccggcaca tcgctttttc gttgaaataa tcccgaaaag aacactattt
     1141 acctccgccg acagactgcc gtgtacggtc gagatatcgc gccaccagcc acgaccagaa
     1201 caggcacagc gccccggcaa caacaaaacc cgccggggga taaatcagcc aggcaccata
     1261 cgccagcaaa agcgcaccca gcacgcccac cagaggcgcg agaatcagca tgatcataat
     1321 tacctcagtt aaagcgagcg gatcccgtag gactcaatgt ggtcagacag cgtgtcttct
     1381 ttctcgtaca gcatggctct gccaaccgcc ataatcagcg caactgcacc atcgattttg
     1441 ttttccgcct gctctttgac gggtttcacc acatcatcgt tacccggcat gtttttgccg
     1501 accacgttgc cgatacacca ggtcatgatg ggattgccgt catgatgaaa acgtcccgat
     1561 tcaatcgctg cttccagctc tttcatcgga tcggacatat tggtgaagtt ctggacgata
     1621 gtgacgggat tcaggtcttc atcagcaagg tcatgtgaca acccggtcgc cccgaagggg
     1681 tcgatgggtg actcactgac cgggctgatt ttgttcgccg ctttggcctc ctcgaggatg
     1741 tagcgataat ccacctccgc accatcggta acggtcagaa cgcccatttc cacccatttc
     1801 tgaaagcgtt cggctgtccg tcgatcttca tttttctcga cgctgtacac cgtgtcatac
     1861 ggtacccaga aacgcggggc cacactgtag taatgcgttt taccgtcaat ctcgcgggta
     1921 taaagtcgcg ccatgctgtt catatccagc ttacgcgcca ggtcaaaggc cagaatgcac
     1981 ggctgcccct cgaactgctc aagggtcagt gatttatcct cgcagctctg ccagctcacc
     2041 aagttgaaat acgccgaacg cgccgacacc cagatattga ggtgttttgt tttaaagacg
     2101 tttgccagac gggcgttatt tttcgcacgc tgctgctgac ttaacaaaaa ttcgcgataa
     2161 accgacacgc caatatttgg attggctttt tccagcacct gcgggccggt ccagtcgtca
     2221 ccttcatcaa cggtatagat gatcccgaac agttcatcgt taggcaccga gccgttgagc
     2281 atctcgatga cttcccgccg cttgtcgtag cacggcccct caatgttgta cccggcggtg
     2341 gtgatggccc acatcagtgg ctgacgtcgc gcccccatcc cggtaagcat tgtggtataa
     2401 agcgcatcgg tggcatgctc gtgatattca tcaaccacgg cacagtgggg tgatgaacca
     2461 tcacctgggt tgccgatcag cggttcaaac cgcgcgccat cctccggacg gttcatgttt
     2521 gaggcgttaa cctcaatccc gaacgcttcc gtcagcatgg gtgtgcgttt acacatcagt
     2581 cgcgccgggc gaaagacttc ccacgcctgt ttctctgtcg tggcaccgga atacacttcc
     2641 gcgccaaact cgttatcaca ggcaaaacaa tacagggcaa caccggcaga gattgctgat
     2701 ttgccgttct tacgggggat ttcggtgtac acctcccgga agcggcgcaa ccgggtgcct
     2761 ttattgaccc agccaaacgc acagcagatc acaaatagct gccacggctc cagcgtgatg
     2821 ggcatccgtt tgaatgccca ctcccccttg gtgtgcggca acagctgaat aaatttcgcg
     2881 gcccgttcag ccaggtcctt gtcgaagcgg taacgaaacg acttactttt ttccgccatc
     2941 aggtcatcaa gatggcgctg gcaggcctga atcacaaact ggcaggcaac aatctttccg
     3001 cgcacgacat cccgggcata ctgatttgca gcatttacgt tggggtaaga tttccggctc
     3061 atgattcgat aattttcaga aacgggttag tggctttctt ctgccccgcc aggccaatca
     3121 gacgctggcg gctgctgggg tcgagtccga gcattgcccc cgtgctgctc atctcggact
     3181 cctgttcttt tttggcggtc agctccggat ttttgaccct gccgcccatt gcaccggtga
     3241 tggtgttgcc ctgtatggca atatttttca cggcacgtcg ccagaactca taggccacgc
     3301 accaccgctc aagtaccgcc aggtcagtca cgcacagcag gccctgaccg cagagttctt
     3361 tggttgtcag ttgccacatg atcgtggcga gagggagatc ttcttcagcg aaccactctg
     3421 gtggctcaac acctttgatg ggcgtaaaaa caggttcatc tttgttcagg gctcgcttgc
     3481 cggggtttcc ggccagcgcc ttgcgcgccg ttggcttggg gcgacgcccg gaacgccccg
     3541 ccgttccagc catatgcggc actcctggtt aaatttcatt tttcgcgggt ataaaaaaac
     3601 gatggggcgg gcagtccgga agacgtcagg tcacagggat ttgccccgcc cctccctaca
     3661 agtgagaata attatcaccg gattcgttcg cgcgctgttt tcgctttatg gcagggccag
     3721 cacagactct gcaggttgct gtctgcgtct gttccgccat gcgctttcgg gatgatgtgg
     3781 tcgacggttt tcgcctcgct caccacaccg acacgcagac acaactgaca cagaccttta
     3841 tcgcgcttca gaatacgggc acgaatcacc gtccattttg agccatagcc acgctggtgg
     3901 cggctcagtc cgcgctggtg ctgcacccag ccttcaccgc gatgtttatc gcagtaaccc
     3961 gaactgtctg tggttgtacc tgcacatcca cgcttacggc aggcacgtgg gattagtgat
     4021 ggcataaata cctcataccc tgcgaaatgt ttaccacgat aaaaaggcta cttaatgcac
     4081 tgagtgcgga tatactcctg tgccccttcc agttgcatct gcatcgtcat cagccgctct
     4141 ctgagggtga aataatcccg ttcagcggtg tctgccagtc gggggctggt tgcattatcc
     4201 acgccggagg cggtggtggc ttcacgcact gactgacaga ctgctttgat gtgcaaccga
     4261 cgacgaccag cggcaacatc agcgcgcaga gtttcatttt cagctttcgc atcagctaac
     4321 tccttcgtgt atttagcatc gagtgcatta acatcacgct gtctctgctg catgtcagta
     4381 atggttgcgt tcgccagctt cagttctctt gcgtttttgt cgcgctgctc tttgtaggta
     4441 atggcgttat cacggtaatg attaacagcc catgacaggc agacgatgat gcagataacc
     4501 agagcggaga taatcgcggt gactctgctc attgctgccc ccacaaacag acttcacgct
     4561 caatctcacg acgggtcatc agccctttcc attgcttacc gccagcatat gtccagcgac
     4621 gtagctggtc acatgcgcct ttgatatcgc cctggtttat tttgcgaaga agcgtcgatg
     4681 ttctgaaatt gccagcgccc acgttgtaaa cgaacgagta aagagcgccg cgcgttgttt
     4741 ccggtatatc gactttgatg tacgggttaa tttgtctggc gacagtggca aggtctttat
     4801 tcaggagggc tttgcattct gcttcggtat acgttttacc gagcatgatg tcttttccgg
     4861 tgtgtccgtg acatacagtc catacaccaa tgatatcttt gtatggtatg tagctgacac
     4921 cttccagacc atcgttacca cctgggccag tgattaacac agatgctata gcaacagccc
     4981 caccaccaat agcagctgca ccagccttgc gtaatgatgg cgacattatt cacctctcgc
     5041 agccttacgc ttgtcttctc ttattttgaa atacagattt gtcagataag tcagaagccc
     5101 cagaagcaga cttcccagca caccaatcgc agcccactgt gatggactga cctgatccaa
     5161 ccactgcaaa aa
//
LOCUS       DAZRUF010000255         5165 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZRUF010000255 DAZRUF010000000
VERSION     DAZRUF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.257"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1310..1519
                     /locus_tag="ACHXOL_003736"
     CDS             1310..1519
                     /locus_tag="ACHXOL_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810607.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
     gene            complement(1585..2118)
                     /locus_tag="ACHXOL_003737"
     CDS             complement(1585..2118)
                     /locus_tag="ACHXOL_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810608.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(2121..3047)
                     /locus_tag="ACHXOL_003738"
     CDS             complement(2121..3047)
                     /locus_tag="ACHXOL_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR6810609.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTAPERVKSPSRGFSMGF"
     gene            3224..3622
                     /locus_tag="ACHXOL_003739"
     CDS             3224..3622
                     /locus_tag="ACHXOL_003739"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810610.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKELKYDLETSPNPDA"
     gene            complement(3826..4740)
                     /locus_tag="ACHXOL_003740"
     CDS             complement(3826..4740)
                     /locus_tag="ACHXOL_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR6810611.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
BASE COUNT         1438 a         1346 c         1108 g         1273 t
ORIGIN      
        1 aaaaaaaaac cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa tattttagtt
       61 atcggatgct caatattcac aaacgcaaaa aatgcctcag tgatgcccta aaacaacctt
      121 tccagaagga gtccgcgctt tacgatgcac ggaagagata aaaaacccca caaaagtggg
      181 gaaccaagag aaacaacaat tacaggttgt tggttgtgtt cgaacaacga tgaagtagct
      241 acgctaaata ataattaccg tacaggccct acatattcaa cactaaattt aagatcctga
      301 cgaaggattt ataaatgcac gaaaacacat agacagacaa attcactaaa ttcatacaca
      361 aaaacaagtg tagttttcta agtaaattag attcgacaac caattagttt atccacatcg
      421 cattccactc ttgctacacc aatcacattt cttgatatgt aatagcagaa aaaataaaac
      481 cagacaccaa tagaccaaaa aacaaacatt caacaagaaa cattttgaac aatatcacaa
      541 aactgtcaca accatcatca gactattgaa aaaaactggt tgctgtttta taaaagcgac
      601 tgtaacaacc tccactctat agttccatta gtttaaagtg tcacacaaac tgagcatcca
      661 tttgttttcc ttacttttgc ccctgtattc caaggggttt ttttataact taaagattag
      721 cacaccacac aaaaacaata aaaaaaaacg acaagttata caataaaacc agtactatcc
      781 atacagctaa ttacatataa atactattaa accatctgga cattaaaaat aaactcttat
      841 aacataaaat taatgcggtt ccggtaccat atgtcaggga taatgtgcaa atggaggtat
      901 cggaactgca tttcagttca ttattaatta atgacaaaaa aaacctctgc gagcgcagag
      961 ggggtaagga atgttcccgg tcataatatg tcgatagtgt ttatatacat acagaacaag
     1021 aacaacaacc agccaactaa taaatatttt attttttaga gctgaaacct taaagctcac
     1081 ttccattcca tacattgtta taaaccttat gttgaaaact gaaagtaagt acggcagact
     1141 tattaagttg cgtgcagtct tttcacgtta tacaaacaag aacacctgcg aagttatttc
     1201 tccttacata tccccctatg ccaacggggg attttttctg gttcactaac gcataaataa
     1261 tagtgacacc ctatcattca caaaccgtga taatataagg attatcatca tggcaaaatt
     1321 aggtgaaaac accccacata ttatcgatgc tactgtagat tttatggcat caagccaggc
     1381 atttcgtgag tatctgaaaa aacttccccc tgataacaca ataccgccag ctcttactga
     1441 tgagcacgta ccgttatata tacgaaggct ggaatactat cgtctgcttt atcgaccaaa
     1501 gcagacagaa tcatattgaa aaaaacggtg aacggttcgc gaaatccttt cgcgggtcgt
     1561 gaggcgttgg acaagcgaag cgcgtcagtt tttccggctc ttccccgtga caggtttctc
     1621 caccagttta tcttccagtg tcacaacttt ctgatacacc gccgctgtgg cgttgtagtt
     1681 cctgtcgagt attgttttca ttccccatcc gagcacacag aacataacca gaaaagccac
     1741 tgacacccca cccagccatt tccagctata ccctttaagg gtgttgtatt cctgcacgat
     1801 attgctgtga attgtcgcaa tatccctgtt cagggcatcc gcgtttgcct tcatgtttgc
     1861 gactgactct tccagccgct gaaaatcctg gctgatgcgc tcacctatca gtcctgccac
     1921 gtccagcttc tgcacctcat ttctgacttc atccctgacc tgttttgcca caccgtcaga
     1981 aatatcccga tatccgcgaa gagtttccct tgccacagcc agagaatcag caacagcttt
     2041 ctgttgctcc attgcggtat gttgcagatg ctccaccgat aacaggagcg tgagtaattt
     2101 ttcttctgtg ctggccatgt tcagaatccc atagaaaatc cacgtgacgg tgatttaacc
     2161 cgttcaggtg cagttcggtt ctgtctgctc actctgtctc tcatgtcaaa gccatgctcc
     2221 cgcgcaacat catgttgcag ccttctgacc agttcatgtt gcttaaccgg agtgtccagc
     2281 cattccctga tgtcatgccc gaccgttcgc gcctgttcaa gatggcggga atagtccggt
     2341 tcagaccgtt tttgcatgat gtcatgacgc tgggcatcat caagccgtcc ggcagcttca
     2401 aaacccagat gcacttcggg acgtcgttca atgccctgtt ctttcagcgt ccggcagtca
     2461 atgaagcccc tttcaaagcc atgctttctc atatgttcgt tgtgcttcac ctcccagcgt
     2521 tttcgcaatt cccgcaactg ctctttttgc tgagtgagag tcaggcttcc ggaatccttt
     2581 ttcgcacctc cccgctcggg atacctggcg ttatagcgcc ggaaatactg ttccggtgta
     2641 cgttcaatgc catcattcac acgctgactc atcatgatat gcgcatgagg ctgttcgccg
     2701 ccgtcaatgc tcgctttcgg attgtgaatg gcaaaactcc aggcgtgccg ttcgccagct
     2761 tcctgacgta caaaatcctg caccagttca aggcgctgtt ccggtgtcag ttcacggggt
     2821 aatgcaattt caagttccct ataggtcgaa ccgttcgcac gttcaaactg gtcagccgcc
     2881 tgccagaaat gggaaggatt atccctggcc cattccggca tgtttccgtg tgcggaatat
     2941 tccaaatcct gacggtcgcg gtatttctct ttgcgctcga tgtaatcggc gtggtttgca
     3001 gcctgcccct tgccaccaaa tttaacgctg aggtgatagg ttgccacggt ttcagccctc
     3061 tgcaggagcg cactgtttct cgacgaagga gagaaacata taagtgcgct tccttctaat
     3121 tttacgaatt agcacggaag ataccccctg cacccgccga ccgcaagcga tatactgcac
     3181 gaccacaaca caccgcaaaa aaacaccagg gagcatcacg aacatgcgca tcagcaacat
     3241 cgaatggctg aagaaacgga tcggattcat caggaagctc ggagaacaga ccgcacgaca
     3301 gagacagatt attgacctga tcgacaacga agccggactc acagaacagg aacgcaaact
     3361 tctccacgta ctggcaacag cggaaaaaaa cgacttacag gcacaggaga gcgaacgaaa
     3421 acaggcggtc caaaaacgaa tcgagggaaa aaaacaacga cgggaacgca atcaccggct
     3481 ctttctcgcg gctgggctac tgatcgaagc aggactggta gacacaaaaa caggcgagct
     3541 atgctataaa aaagacagga tattacaggc acttaaggaa cttaagtacg atctggagac
     3601 atccccaaac ccagacgcgt aagtgcctcc atccgtgttc cgattccctg gtgcttcctc
     3661 gctcattcgc tcgctacgct cggtcatgag actgcggcga gcactaccac cacaatcacg
     3721 cggaaagccg cgccattgca gggctgaacg gggaaaaacg gcaaaaaacc tgctgctcaa
     3781 aaagacgcca gaaaggcgct gttatctgat ataggcttat ttggtctact ctacgagacc
     3841 tcgttttttc agttggtaat accaggcgcg actgatccct aatgcctccc acggtttcag
     3901 cgtgcgtgca gatgtagcta ctgtgctgcg cttagactta gaaccgccct tgcgaccgcg
     3961 tgcagcctga atttctggcg tgtgcgtatc agccacatac tgtgcgaaag tttccggcgt
     4021 gaagttcctg tgcgtgtatt tcgcaatgct cttgccaata gcccgacatt cagccagtga
     4081 aagcggaacg ggaagcgatg cattgtacat ttcgacacgc tggatcaccg cttcaagcca
     4141 ctgtgagaac gctggccagc cctgacgaat cgccctgtaa gcccatttgc gcgtcatttc
     4201 gaacaggtgg cagtttcgcc ccatcccgta atgtttatcg atgctacgac gctctgaggc
     4261 gctcaaatcg agataatcag ccagttcatc gagggtatag gcttcctcgc gccactccat
     4321 caccagccat tccaggtgga acggattttt gcaaatcagg ccgctgtagt tcacatccgc
     4381 acaaagtttc tcacacagcg aacgttcaac cgctgcggca tatttcagcg ccttaaccga
     4441 ggcatcaggc gcggttctca cggcgatgtt gagtgcgtag agcaggtgag cgtgtccgtt
     4501 cacaggattt ttaacggtga tgttcggcgc gggagcgttc cggtcgctcc agtcaatcgc
     4561 agccccctcg cggtcaacgt caaaaaccag ccagaactgt gcatgaggct ggttctgctg
     4621 gatataccgt gcaagaagcg cgcgcccttt gccggagata cgaaggccga aagcgagatc
     4681 atccgtgtga tagggacggt ggggtaaatt ttcttcgaag tattgaagcg cggcgctcat
     4741 gtgttacgat tccttccagg ttatcgaccg acatcgcccc tgtattttcg tgaaagtctt
     4801 ggcggataga cacgaatttc aggggcgttt gtttttcagt cgcttacact acccccaacg
     4861 acaaaacctg tcgagccttc ccgcaaatcc gatccattta ccataactcc cgttgtacgt
     4921 accatgtaaa acgccctcaa tcgctttctg gcgcaaaaaa cgcaaaaaga caagaaaaca
     4981 ccaggccaaa ccagaaaaac gcgtcagaaa cgttttcagg gcgttgcata actatgcacg
     5041 aaagcgatgt gcgatcggtt tcgtgcgttc tgaacaccga acggctcaaa acgacaagtt
     5101 ttctcccctg gtgcgtggcg ctctgacggg gtttcctgtt tccaaacttc acatctcgcc
     5161 aggac
//
LOCUS       DAZRUF010000256         5088 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZRUF010000256 DAZRUF010000000
VERSION     DAZRUF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.258"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..741
                     /gene="hdhA"
                     /locus_tag="ACHXOL_003741"
     CDS             <1..741
                     /gene="hdhA"
                     /locus_tag="ACHXOL_003741"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR6810612.1"
                     /translation="DGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVD
                     EIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADF
                     RRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHL
                     VRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANA
                     ALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            962..1552
                     /gene="uidR"
                     /locus_tag="ACHXOL_003742"
     CDS             962..1552
                     /gene="uidR"
                     /locus_tag="ACHXOL_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR6810613.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1941..3752
                     /gene="uidA"
                     /locus_tag="ACHXOL_003743"
     CDS             1941..3752
                     /gene="uidA"
                     /locus_tag="ACHXOL_003743"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR6810614.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFERVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3749..>5088
                     /gene="uidB"
                     /locus_tag="ACHXOL_003744"
     CDS             3749..>5088
                     /gene="uidB"
                     /locus_tag="ACHXOL_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR6810615.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKV"
BASE COUNT         1243 a         1201 c         1342 g         1302 t
ORIGIN      
        1 gacggaaaat gcgccatcat cacaggtgcg ggtgcaggta ttggtaaaga aatcgccatt
       61 acattcgcga cagctggcgc atctgtggtg gtcagtgata ttaacgccga cgcagctaac
      121 catgttgtag acgaaattca acaactgggt ggtcaggcat ttgcctgccg ttgtgatatt
      181 acttccgaac aggaactctc tgcactggca gactttgcta tcagtaagct gggtaaagtt
      241 gatattctgg ttaacaacgc cggtggcggt ggacctaaac cgtttgatat gccaatggcg
      301 gattttcgcc gtgcttatga actgaatgtg ttttcttttt tccatctgtc acaacttgtt
      361 gcgccagaaa tggaaaaaaa tggcggtggc gttattctga ccatcacttc tatggcggca
      421 gaaaataaaa atataaacat gacttcctat gcatcatcta aagctgcagc cagtcatctg
      481 gtcagaaata tggcgtttga cctgggtgaa aaaaatattc gggtaaatgg cattgcgccg
      541 ggggcaatat taaccgatgc cctgaaatcc gttattacac cagaaattga acaaaaaatg
      601 ttacagcaca cgccgatcag acgtctgggc caaccgcaag atattgctaa cgcagcgctg
      661 ttcctttgct cgcctgctgc gagctgggta agcggacaaa ttctcaccgt ctccggtggt
      721 ggggtacagg agctcaatta atacactaac ggaccggtaa acaaccgtgc gtgttgttta
      781 ccgggataaa ctcatcaacg tctctgctaa ataactggca gccaaatcac ggctattggt
      841 taaccaattt cagagtgaaa agtatacgaa tagagtgtgc cttcgcacta ttcaacagca
      901 atgataggcg ctcacctgac aacgcggtaa actagttatt cacgctaact ataatggttt
      961 aatgatggat aacatgcaga ctgaagcaca accgacacgg acccggatcc tcaatgctgc
     1021 cagagagatt ttttcagaaa atggatttca cagtgcctcg atgaaagcca tctgtaaatc
     1081 ttgcgccatt agtcccggga cgctctatca ccatttcatc tccaaagaag ccttgattca
     1141 ggcgattatc ttacaggacc aggagagggc gctggctcgt ttccgggaac cgattaaagg
     1201 gattcatttt gttgactata tggtcgagtc tattgtctct ctcacccatg aagcctttgg
     1261 gcaacgggcg ctggtggtcg aaattatggc ggaagggatg cgtaacccac aggtcgccgc
     1321 catgcttaaa aataagcata tgacgatcac ggaatttgtt gcccagcgga tgcgtgatgc
     1381 ccagcaaaaa ggcgagataa gcccagacat caacacgtca atgacttcac gtttactgct
     1441 ggatctgacc tacggtgtac tggccgatat cgaagcggaa gatctggcgc gtgaagcgtc
     1501 gtttgctcag ggattacgcg cgatgattgg cggtatctta accgcatcct gatcctctca
     1561 tttcgcgggc tggtgataac tgtgcccgcg tttcatactt taatttccct gtacaaaata
     1621 caatctagcc gggatcggag aattcccccc caaaaatatt caccgtagcc atatgtcatg
     1681 agagtttatc gttcccaata tgctcgaacg aacgttcggt tgcttatttt atggcttctg
     1741 tcaacgccgt tttaaagatt aatgcgatca atatcacgct gtgggtattg cagtttttgg
     1801 ttttttgatc gcggtgtcag ttctttttat ttccatttct cttccatggg tttctcacag
     1861 ataactgtgt gcaacacaga attggttaac taatcagatt aaaggttgac cagtattttt
     1921 atcttaatga ggagtccctt atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa
     1981 aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt
     2041 ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc
     2101 agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct
     2161 ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
     2221 acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat
     2281 ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg
     2341 tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg
     2401 gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccgggatc catcgcagcg
     2461 taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg
     2521 cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
     2581 ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt
     2641 tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg
     2701 tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag
     2761 tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg
     2821 gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg atggtgcacg
     2881 accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
     2941 ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg
     3001 ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac
     3061 tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag
     3121 agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac
     3181 cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta
     3241 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     3301 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     3361 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     3421 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     3481 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     3541 accgcgtctt tgagcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     3601 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaggggatc ttcacccgcg
     3661 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     3721 gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt
     3781 cggctacagc ctcggtgacg tcgccaataa cttcgccttc gcaatggggg tgctcttcct
     3841 gttgagttac tacaccgacg tcgctggcgt cggtgccgct gcggcgggca ccatgctgtt
     3901 actggtgcgg gtattcgatg ccttcgccga cgtctttgcc ggacgagtgg tggacagtgt
     3961 gaatacccgc tggggaaaat tccgcccgtt tttactcttc ggtactgcgc cgttaatgat
     4021 cttcagcgtg ctggtattct gggtaccgac cgactggagc catagccgca aagtagtgta
     4081 tgcatatttg acctacatgg gcctcgggct ttgctacagc ctggtgaata ttccttatgg
     4141 ttcacttgct accgcgatga cccaacaacc acaatcccgc gcccgtctgg gcgcggctcg
     4201 tgggattgcc gcttcattga cctttgtctg cctggcattt ctgatagggc cgagcattaa
     4261 gaactccagc ccggaagaga tggtgtcggt ataccatttc tggacgattg tgctggcgat
     4321 tgccggaatg gtgctttact tcatctgctt caaatcgacg cgtgagaatg tggtacgtat
     4381 cgttgcgcag ccgtcattga agatcagttt gcaaaccctg aaacggaatc gcccgctgtt
     4441 tatgttgtgc atcggtgcgc tgtgtgtgct gatttcgacc tttgcggtca gcgcctcgtc
     4501 gttgttctac gtgcgctatg tgttaaatga taccgggctg ttcactgtgc tggtactggt
     4561 gcaaaacctg gttggtactg tggcatcggc accgcttgtg ccggggatgg tcgcgaggat
     4621 cggtaaaaag aataccttcc tgattggcgc tttgctggga acctgcggtt atctgctatt
     4681 cttctgggtt tccgtctggt cactgccggt ggcgttggtt gcgttggcca tcgcttcaat
     4741 tggtcagggc gttactatga cagtgatgtg ggcactggaa gctgataccg tagaatacgg
     4801 tgaatacctg accggcgtgc gaattgaagg gctcacctat tcactattct catttacccg
     4861 taaatgcggt caggcaatcg gaggttcaat tcctgccttt attttggggt taagcggata
     4921 tattgccaat caggtgcaaa cgccggaagt tattatgggc atccgcacat caattgcctt
     4981 agtaccttgc ggatttatgc tactggcatt cgttattatc tggttttatc cgcttacgga
     5041 taaaaaattc aaagaaatcg tggttgaaat tgataatcgt aaaaaagt
//
LOCUS       DAZRUF010000257         4980 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZRUF010000257 DAZRUF010000000
VERSION     DAZRUF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.259"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(3..146)
                     /gene="ypaB"
                     /locus_tag="ACHXOL_003745"
     CDS             complement(3..146)
                     /gene="ypaB"
                     /locus_tag="ACHXOL_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR6810616.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            274..2559
                     /gene="nrdA"
                     /locus_tag="ACHXOL_003746"
     CDS             274..2559
                     /gene="nrdA"
                     /locus_tag="ACHXOL_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR6810617.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2793..3923
                     /gene="nrdB"
                     /locus_tag="ACHXOL_003747"
     CDS             2793..3923
                     /gene="nrdB"
                     /locus_tag="ACHXOL_003747"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR6810618.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            3923..4177
                     /gene="yfaE"
                     /locus_tag="ACHXOL_003748"
     CDS             3923..4177
                     /gene="yfaE"
                     /locus_tag="ACHXOL_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR6810619.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4231..4880)
                     /gene="inaA"
                     /locus_tag="ACHXOL_003749"
                     /pseudo
     CDS             complement(4231..4880)
                     /gene="inaA"
                     /locus_tag="ACHXOL_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
BASE COUNT         1216 a         1342 c         1243 g         1179 t
ORIGIN      
        1 gttcaagaaa tcgccgaaca gttattttta acaaattttt ctcttcccat tgactttccc
       61 ggacaccttg tctgacctaa ggtgcgcgaa agccactttt tccttcctga gttatccaca
      121 aagttatgca cttgcaagag ggtcattttc acactatctt gcagtgaatc ccaaacatac
      181 cccctatata tagtgttcta agcagcttcc cttactacag gtagtctgca tgaaactatt
      241 gcggaaagaa ttccaaaaac aggtacgaca tacatgaatc agaatctgct ggtgacaaag
      301 cgcgacggta gcacagagcg catcaatctc gacaaaatcc atcgcgttct ggattgggcg
      361 gcagaaggac tgcataacgt ttcgatttcc caggtcgagc tgcgctccca cattcagttt
      421 tatgacggta tcaagacctc tgacatccac gaaaccatta tcaaggctgc cgcagacctg
      481 atctcccgtg atgcgccgga ttatcagtat ctcgccgcgc gcctggcgat cttccacctg
      541 cgtaaaaaag cctacggcca gtttgagccg cctgcgctgt acgaccacgt ggtgaaaatg
      601 gtcgagatgg gcaaatacga taatcatctg ctggaagact acacggaaga agagttcaag
      661 cagatggaca cgtttatcga tcacgaccgt gatatgacct tctcttatgc tgccgttaag
      721 cagctggaag gcaaatatct ggtacagaac cgcgtgaccg gcgaaatcta tgagagcgcc
      781 cagttccttt atattctggt tgccgcgtgc ctgttctcga actacccgcg tgaaacgcgc
      841 ctgcaatatg tgaagcgttt ttacgacgcg gtttccacat ttaaaatttc gctgccgacg
      901 ccaatcatgt ccggcgtgcg taccccgact cgtcagttca gctcctgcgt actgatcgag
      961 tgcggtgaca gcctggattc catcaacgcc acctccagcg cgattgttaa atacgtttcc
     1021 cagcgtgccg ggatcggcat caacgccggg cgtattcgtg cgctgggtag cccgattcgc
     1081 ggtggtgaag cgttccatac cggctgcatt ccgttctaca aacatttcca gacagcggtg
     1141 aaatcctgct ctcagggcgg tgtgcgcggc ggtgcggcaa cgctgttcta cccgatgtgg
     1201 catctggaag tggaaagcct gctggtgttg aaaaacaacc gtggtgtgga aggcaaccgc
     1261 gtgcgtcata tggactacgg ggtacaaatc aacaaactga tgtatacccg tctgctgaaa
     1321 ggtgaagata tcaccctgtt cagcccgtcc gacgtaccgg ggctgtacga cgcgttcttc
     1381 gccgatcagg aagagtttga acgtctgtat accaaatatg agaaagacga cagcatccgc
     1441 aagcagcgtg tgaaagccgt tgagctgttc tcgctgatga tgcaggaacg tgcgtctacc
     1501 ggtcgtatct atattcagaa cgttgaccac tgcaataccc atagcccgtt tgatccggcc
     1561 atcgcgccag tgcgtcagtc taacctgtgc ctggagatag ccctgccgac caaaccgctg
     1621 aacgacgtca acgacgagaa cggtgaaatc gcgctgtgta cgctgtctgc tttcaacctg
     1681 ggcgcaatta ataacctgga tgaactggaa gagctggcaa ttctggcggt tcgtgcactt
     1741 gacgcgctgc tggattatca ggattacccg atcccggccg ccaaacgtgg agcgatgggt
     1801 cgtcgtacgc tgggtattgg tgtgatcaac ttcgcttact acctggcgaa gcacggtaaa
     1861 cgctactccg acggcagcgc caacaacctg acgcataaaa ccttcgaagc cattcagtat
     1921 tacctgctga aagcctctaa tgagctggcg aaagagcaag gcgcgtgccc gtggtttaac
     1981 gaaaccactt acgcgaaagg gatcctgccg atcgatacct ataagaaaga tctggatacc
     2041 atcgctaatg agccgctgca ttacgactgg gaagcattgc gtgaatcaat caaaacgcac
     2101 ggcctgcgta actctacgct ttctgccctg atgccgtccg agacctcttc gcagatctct
     2161 aacgccacta acggtattga accgccgcgc ggttacgtca gcatcaaagc gtcgaaagac
     2221 ggtattttgc gccaggtggt gccggactac gagcacctgc acgacgccta tgagctgctg
     2281 tgggaaatgc cgggtaacga tggctacctg caactggtag gtatcatgca gaaatttatc
     2341 gatcagtcga tctctgctaa caccaactac gatccgtcac gtttcccgtc aggaaaagtg
     2401 ccgatgcagc agttgctgaa agacctgctc accgcctaca aattcggggt caaaacgctg
     2461 tattatcaga acacccgtga cggcgctgaa gacgcgcagg acgatctggt accgtcaatc
     2521 caggacgatg gctgcgaaag cggcgcatgt aagatctgat attgagatgc cggatgcggc
     2581 gtaaacgcct tatccggcct acggctcggt ttgtaggcct gataagacgc gccagcgtcg
     2641 catcaggctc cgggtgccgg atgcagcgtg aacgccttat ccggcctacg gctcggattt
     2701 gtaggcctga taagacgcgc cagcgtcgca tcaggcacag gatgcggcgt aaaatgcctt
     2761 atccggcatt aaactcccaa caggacacac tcatggcata taccaccttt tcacagacga
     2821 aaaatgatca gctcaaagaa ccgatgttct ttggtcagcc ggtcaacgtg gctcgctacg
     2881 atcagcaaaa atatgacatc ttcgaaaagc tgatcgaaaa gcagctctct ttcttctggc
     2941 gtccggaaga agttgacgtc tcccgcgacc gtatagatta ccaggcgctg ccggagcacg
     3001 aaaaacacat ctttatcagc aacctgaaat atcagacgct gctggattcc attcagggtc
     3061 gtagcccgaa cgtggcgcta ttgccgctta tttctattcc ggaactggaa acctgggtcg
     3121 aaacctgggc gttctcagaa acgattcatt cccgttccta tactcatatc attcgtaata
     3181 tcgttaacga tccgtctgtt gtgtttgacg atatcgtcac caacgagcag atccagaaac
     3241 gtgcggaagg gatctccagc tattacgatg aactgatcga aatgaccagc tactggcacc
     3301 ttctgggcga aggcacccac accgttaacg gtaaaaccgt aaccgttagc ctgcgcgagc
     3361 tgaagaaaaa actgtatctc tgcctgatga gcgttaacgc gctggaagcg atccgcttct
     3421 acgtcagctt tgcttgttcc tttgcctttg cagaacgcga actgatggaa ggtaacgcca
     3481 agattattcg cctgattgcc cgcgatgaag ccctgcacct gaccggtact cagcatatgc
     3541 tgaatctgct gcgcagcggc gcggatgatc cggagatggc ggaaattgcc gaagaatgta
     3601 agcaggagtg ctatgacctg tttgtccagg cggcacagca agagaaagac tgggcggatt
     3661 atctgttccg tgacggttcg atgattggtc tgaataaaga tattctctgc cagtacgttg
     3721 aatacatcac caatatccgc atgcaggcag tcggtttgga tctgccgttc cagacgcgct
     3781 ccaacccgat cccgtggatc aacacctggc tggtgtctga taacgtgcag gttgctccgc
     3841 aggaagtgga agtcagttct tatctggtcg ggcagattga cgcggaagtg gacaccgacg
     3901 atttgagtaa cttccagctc tgatggcccg cgttaccctg cgcatcactg gcacacaact
     3961 gctgtgccag gatgaacacc cttcccttct ggcggcgctg gaatcccaca atgttgcggt
     4021 tgagtaccag tgccgcgaag gttactgcgg ctcctgtcgc acccgactgg tcgcaggtca
     4081 ggtggactgg attgccgaac cgttagcctt tattcagccg ggggaaattt tgccctgttg
     4141 ttgccgggca aaaggcgata ttgaaatcga gatgtgaatt ggttgtagtg ccagatacaa
     4201 cgcttatacg tcttatctgg cctacaacga ttacatggcg tagtaatacg ctttcacctg
     4261 ctcccagtcc gctttgggga ttggctccag atattttcca gctggcggaa gtcatgatta
     4321 atcgctttat tgcgacgcaa gcggcgacga cttttctcca ggtcaagaaa accagcttct
     4381 gctttacctt ctgttttcac atagatatgg cgaacataac aacagccatg ctgacgatta
     4441 acgctatgca ttttcttaaa cgccagcgcc actgctttca acatggcttg ccgtacttcg
     4501 tcagaatagg gcgatactgc atgccgggca taccagtcag caatgctgat gaaccccgcc
     4561 atatcttcag tcaccagcaa cgcgcgccat tcaccctcaa ttttcaccgc ttcaccaaaa
     4621 acgatcttcg gcacgatgac accggcccgt tccagttctt taattaccgc aacctcacgg
     4681 acaatcgttg gtcggccgaa cggataacgt acggaatgaa acagatgatg cgtcatgcgc
     4741 tttacataca gctttttgcc gttgcgctcg acgcattgca ccccgctcat accattacgg
     4801 cgatagttag gctcttcaac ccagtcgccc tctgttgccc accagtgatt aaattcgtcg
     4861 tactttgctg aaactgccat accctatcgc ctgtcatttt tattaacgac aatgactata
     4921 ggtggttacc tgaggaaaat cttaatgaaa cgtgtcgtat taatgaattt cttttgccgc
//
LOCUS       DAZRUF010000258         4930 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZRUF010000258 DAZRUF010000000
VERSION     DAZRUF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.260"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            224..1168
                     /gene="leuO"
                     /locus_tag="ACHXOL_003750"
     CDS             224..1168
                     /gene="leuO"
                     /locus_tag="ACHXOL_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR6810620.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1323..1367)
                     /locus_tag="ACHXOL_003751"
     CDS             complement(1323..1367)
                     /locus_tag="ACHXOL_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810621.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1486..3210
                     /gene="ilvI"
                     /locus_tag="ACHXOL_003752"
     CDS             1486..3210
                     /gene="ilvI"
                     /locus_tag="ACHXOL_003752"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR6810622.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3213..3704
                     /gene="ilvN"
                     /locus_tag="ACHXOL_003753"
     CDS             3213..3704
                     /gene="ilvN"
                     /locus_tag="ACHXOL_003753"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR6810623.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3716..3802
                     /locus_tag="ACHXOL_003754"
     CDS             3716..3802
                     /locus_tag="ACHXOL_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR6810624.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3884..4888
                     /gene="cra"
                     /locus_tag="ACHXOL_003755"
     CDS             3884..4888
                     /gene="cra"
                     /locus_tag="ACHXOL_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR6810625.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1198 a         1120 c         1300 g         1312 t
ORIGIN      
        1 gattgttttc gcaacaaaaa cattatggat tcttatgctg tggtaaatga ctcattccac
       61 ggcaatggat tctgttttta tcagaacccg tatctttatg ttttccgaat tttactcatt
      121 ttgctttttc ttattttata tgcatgataa atcatattct tcaggattat ttctctgcat
      181 tccaataagg gaaagggagt taagtgtgac agtggagtta agtatgccag aggtacaaac
      241 agatcattca gagacggcgg agttaagcaa gccacagcta cgcatggtcg atctcaactt
      301 attaaccgtt ttcgatgccg tgatgcagga gcaaaacatt acccgtgccg ctcatgttct
      361 gggtatgtcg caacctgcgg tcagtaacgc tgttgcacgc ctgaaggtga tgtttaatga
      421 cgagcttttt gttcgttatg gccgtggtat tcaaccgact gctcgcgcat ttcaactttt
      481 tggctcagtt cgtcaggcat tgcaactagt acaaaatgaa ttgcctggtt caggttttga
      541 acccgcgagc agtgaacgtg tatttcatct ttgtgtttgc agcccgttag acagcattct
      601 gacctcgcag atttataatc acattgagca gattgcgcca aatatacatg ttatgttcaa
      661 gtcttcatta aatcagaaca ctgaacatca gctgcgttat caggaaacgg agtttgtgat
      721 tagttatgaa gacttccatc gtcctgaatt taccagcgtg ccattattta aagatgaaat
      781 ggtgctggta gccagcaaaa atcatccaac aattaagggc ccgttactga aacatgatgt
      841 ttataacgaa caacatgcgg cggtttcgct cgatcgtttc gcgtcattta gtcaaccttg
      901 gtatgacacg gtagataagc aagccagtat cgcgtatcag ggcatggcaa tgatgagcgt
      961 acttagcgtg gtgtcgcaaa cgcatttggt cgctattgcg ccgcgttggc tggctgaaga
     1021 gttcgctgaa tccttagaat tacaggtatt accgctgccg ttaaaacaaa atagcagaac
     1081 ctgttatctc tcctggcatg aagctgccgg gcgcgataaa ggccatcagt ggatggaaga
     1141 gcaattagtc tcaatttgca aacgctaact gaatgcagaa taggtcagac atgaatgtct
     1201 ggtttattct gtatttttta ttgaatgtag aattttattc tgaatgtgtg ggctctctat
     1261 tttaggatta attaaaaaaa tagagaaatt gctgtaagtt gtgggattca gccgatttat
     1321 tatcaattta atccttcgta atggaggatt ttatcgtttc ttttcacctt tcctcctgtt
     1381 tattcttatt accctgcgtt tatgtctctg gctgccaatt gcttaagcaa gatcggacgg
     1441 ttaatgtgtt ttacacattt tttccgtcaa acagtgaggc aggccatgga gatgttgtct
     1501 ggagccgaga tggtcgtccg atcgcttatc gatcagggcg ttaaacaagt attcggttat
     1561 cccggaggcg cagtccttga tatttacgat gcattgcata ccgtgggtgg tatcgatcat
     1621 gttttagttc gtcatgagca ggcggcagtg catatggccg atggtctggc gcgcgcgacc
     1681 ggggaagtcg gcgtcgtgct ggtaacgtcg ggtccagggg cgaccaatgc gattactggc
     1741 atcgccaccg cttatatgga ttccattcca ttagttgtcc tttccgggca ggtagcgacc
     1801 tcgttgatag gttacgatgc ctttcaggag tgcgacatgg tggggatttc gcgaccagtg
     1861 gttaaacaca gttttctggt taagcaaacg gaagacattc cgcaggtgct gaaaaaggct
     1921 ttctggctgg cggcaagtgg tcgtcctgga ccagtagtcg ttgatttacc gaaagatatt
     1981 cttaatccgg cgaacaaatt accctatgtc tggccggagt cggtcagtat gcgttcttac
     2041 aatcccacta ctaccggaca taaagggcaa attaagcgcg ctctgcaaac gctggtagcg
     2101 gcaaaaaaac cggttgtcta cgtaggcggt ggggcaatca cggcgggctg ccatcagcag
     2161 ttgaaagaaa cggtggaggc gttgaatctg cccgttgttt cctcattgat ggggctggga
     2221 gcgtttccgg caacgcatcg tcaggcactg ggcatgctgg gaatgcacgg tacctacgaa
     2281 gccaatatga cgatgcataa cgcggatgtg attttcgccg tcggggtacg atttgatgac
     2341 cgaacgacga acaatctggc aaagtactgc ccaaatgcca ctgttctgca tatcgatatt
     2401 gatcctactt ccatttctaa aaccgtgact gcggatatcc cgattgtggg ggatgctcgc
     2461 caggtcctcg aacaaatgct tgaactcttg tcgcaagaat ccgcccatca accactggat
     2521 gagatccgcg actggtggca gcaaattgaa cagtggcgcg ctcgtcagtg cctgaaatat
     2581 gacactcaca gtgaaaagat taaaccgcag gcggtgatcg agactctttg gcggttgacg
     2641 aagggagacg cttacgtgac gtccgatgtc gggcagcacc agatgtttgc tgcactttat
     2701 tatccattcg acaaaccgcg tcgctggatc aattccggtg gcctcggcac gatgggtttt
     2761 ggtttacctg cggcactggg cgtcaaaatg gcgttgccag aagaaaccgt ggtttgcgtc
     2821 actggcgacg gcagtattca gatgaacatc caggaactgt ctaccgcgtt gcaatacgag
     2881 ttgcccgtac tggtggtgaa tctcaataac cgctatctgg ggatggtgaa gcagtggcag
     2941 gacatgatct attccggccg tcattcacaa tcttatatgc aatcgctacc cgatttcgtc
     3001 cgtctggcgg aagcctatgg tcacgtcggg atccagattt ctcatccgca tgagctggaa
     3061 agcaaactta gcgaggcgct ggaacaggtg cacaataatc gcctggtgtt tgttgatgtt
     3121 accgtcgatg gcagcgagca cgtctacccg atgcagattc gcgggggcgg aatggatgaa
     3181 atgtggttaa gcaaaacgga gagaacctga ttatgcgccg gatattatca gtcttactcg
     3241 aaaatgaatc aggcgcgtta tcccgcgtga ttggcctttt ttcccagcgt ggctacaaca
     3301 ttgaaagcct gaccgttgcg tcaaccgatg atccgacatt atcgcgtatg accatccaga
     3361 ccgtgggcga tgaaaaagta cttgagcaga tcgaaaagca attacacaag ctggtcgatg
     3421 tcttgcgcgt gagtgagttg gggcagggcg cgcatgttga gcgggaaatc atgctggtga
     3481 aaattcaggc cagcggttac gggcgtgacg aagtgaaacg taatacggaa atattccgtg
     3541 ggcaaattat cgatgtcaca ccctcgcttt ataccgttca attagcaggc accagcgata
     3601 agcttgatgc atttttagca tcgattcgcg aggtggcgaa aattgtggaa gttgctcgtt
     3661 ctggtgtggt cggactttcg cgcggcgata aaataatgcg ttgagaatga tctgaatgcg
     3721 caatttgcag cccaacatgt cacgttgggc tttttttgcg aaatcagtgg gaacctggaa
     3781 taaaagcagt tgccgcagtt aattttatgc gcttagatgt taatgaattt aacccatacc
     3841 agtacaatgg ctatggtttt tacattttac gcaaggggca attgtgaaac tggatgaaat
     3901 cgctcggctg gcgggagtgt cgcggaccac tgcaagctat gttattaacg gcaaagcgaa
     3961 gcaataccgt gtgagcgaca aaaccgttga aaaagtcatg gctgtggtgc gtgagcacaa
     4021 ttaccacccg aacgccgtgg cagctgggct tcgtgctgga cgcacacgtt ctattggtct
     4081 tgtgatcccc gatctggaga acaccagcta tacccgcatc gctaactatc ttgaacgcca
     4141 ggcgcggcaa cggggttatc aactgctaat tgcctgctca gaagatcagc cagacaacga
     4201 aatgcgctgt attgagcacc ttttacagcg tcaggtcgat gccattattg tttcgacgtc
     4261 gttgcctcct gagcatcctt tttatcaacg ctgggctaac gacccgttcc cgattgtcgc
     4321 gctggaccgc gccctcgatc gtgaacactt caccagcgtg gttggtgccg atcaggatga
     4381 tgccgaaatg ctggcggaag agttacgtaa gtttcccgcc gagacggtgc tttatcttgg
     4441 cgcgctgccg gagctttctg tcagcttcct gcgtgaacaa ggtttccgta ctgcctggaa
     4501 agatgatccg cgcgaagtgc atttcctgta tgccaacagc tatgagcggg aggtggctgc
     4561 ccagttattc gaaaaatggc tggaaacgca tccgatgccg caggcgctgt tcacaacgtc
     4621 gtttgcgttg ttgcaagggg tgatggatgt cacgctacgt cgcgatggta aactgccttc
     4681 tgacctggca attgccacct ttggcgataa cgaactgctc gacttcttac agtgcccggt
     4741 gctggcagtg gctcaacgtc accgcgatgt cgcagagcgt gtgctggaga ttgtcctggc
     4801 aagcctggac gaaccgcgta agccaaaacc tggtttaacg cgcattaaac gtaatctcta
     4861 tcgccgcggt gtgctcagcc gtagctaagc cgcgaacaaa aatacgcgcc aggtgaattt
     4921 ccctctggcg
//
LOCUS       DAZRUF010000259         4927 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZRUF010000259 DAZRUF010000000
VERSION     DAZRUF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.261"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..538
                     /locus_tag="ACHXOL_003756"
     CDS             <1..538
                     /locus_tag="ACHXOL_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR6810626.1"
                     /translation="KSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDED
                     LTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTD
                     LLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPGK
                     DATPGNVYAQADYKIAYE"
     gene            598..3102
                     /locus_tag="ACHXOL_003757"
     CDS             598..3102
                     /locus_tag="ACHXOL_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR6810627.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYMNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSASLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3118..3867
                     /locus_tag="ACHXOL_003758"
     CDS             3118..3867
                     /locus_tag="ACHXOL_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR6810628.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3869..>4927
                     /locus_tag="ACHXOL_003759"
     CDS             3869..>4927
                     /locus_tag="ACHXOL_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810629.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT         1526 a         1010 c         1017 g         1374 t
ORIGIN      
        1 aaagtcactt cttgttgctg gcgtagcaat gattctttcc aataacgtat ttgctgacga
       61 aggtcacggc attgttaaat ttaaaggtga agttatctct gctccatgtt caattaaacc
      121 aggtgacgaa gacttaactg taaatctggg tgaagtagca gataccgttc tgaaaagcga
      181 ccagaaatct ttggctgagc ctttcactat tcacctgcaa gactgcatgt tgagccaggg
      241 tggaaccact tattcaaaag cgaaggttac tttcaccaca gcgaatacta tgacgggtca
      301 gactgacctg ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag gcgttggcgt
      361 acgtattctg gatagccaga gcggcgaagt gactttaggt actccggtag taattacatt
      421 caacaatact aacagctatc aggaactgaa cttcaaagct cgtatggaat ccccgggcaa
      481 agatgctacc ccgggcaacg tttacgctca ggctgactat aagattgctt acgagtaatc
      541 acattaggcc gggttcgccc ggccacatca ttaacggatt aatgataagt ggaacagatg
      601 tataaaaaat taaaattaac cacaataagc gaattgatta aaaatattta ttgttcatta
      661 tccgttctca tcattggttg tgcgtcagct tatgccgttg aattcaacaa agatttaatc
      721 gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg ccaattaccc
      781 gtaggtaaat actcattaaa cgctcttatt aataataaaa ggacgccaat ccatctggac
      841 ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga gcaattaaca
      901 ttattaggct ttactgatga gattattgaa gaggctcagc aaaacctgat cgatggttgt
      961 taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa aatgcaatta
     1021 tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac gcctcctgaa
     1081 ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc ctctcattat
     1141 gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc tggggttaat
     1201 cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa caatggcaaa
     1261 agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat cccagcaatt
     1321 aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga ctctttccat
     1381 ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct acgtggttac
     1441 gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc acagaacaac
     1501 cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa tttattcaat
     1561 acattacagg ggcaactgga cgtcaaggtt gaagaagagg acgggcaagt cacgcaatgg
     1621 caagttgcat ctaatagtat tccttatctg acgcgtaaag ggcagattcg ctacaccact
     1681 gctatgggta aaccgaccag cgttggtggt gattccttac aacaaccctt cttctggact
     1741 ggtgaattct catggggttg gctgaacaat gtatccctgt atggtggttc agttttaaca
     1801 aaccgtgatt atcaatctct ggctgccggc gttggcttta accttaactc attgggttca
     1861 ttatcttttg atgtcacacg atctgatgct cagttgcata atcaggataa agaaacgggt
     1921 tatagctacc gcgctaacta ttcaaaacgt tttgaatcta ccggtagcca gctcactttc
     1981 gctggttacc gtttctctga taaaaacttt gtgacaatga atgaatatat caatgacact
     2041 aaccattaca tgaattatca gaatgaaaaa gagagttata ttgtcacgtt taaccagtat
     2101 cttgaatcat taaggttaaa tacatacgta agtttggctc gtaatactta ctgggacgcc
     2161 agcagtaatg tgaattattc attatcactt agccgcgatt ttgatatcgg gccattaaaa
     2221 aacgtctccg cttctctaac atttagccga ataaactggg aagaagacaa ccaggatcaa
     2281 ctgtacctaa atatttcgat tccctgggga accagtagaa cattgagcta tggtatgcaa
     2341 cgaaatcagg ataacaagat ttcgcatact gcttcgtggt atgactcttc cgatcgaaat
     2401 aattcctgga gcgtttctgc ttcaggcgac aatgatgaat tcaaagatat gaaagcgtca
     2461 ctacgcgcca gttatcagca taataccgag aacggtcgtc tctatctctc cgggacatca
     2521 cagcgagaca gttattattc tctgaatgcc agttggaatg gttcattcac ggcgactcgt
     2581 cacggagccg ctttccatga ctatagtggt agtgctgact cgcgttttat gatcgacgca
     2641 gatggtgcag aagatattcc gttgaacaat aaacgcgcgg taactaatcg gtatggcatc
     2701 ggagttattc catcagtcag cagttacata acaacatcat taagcgttga tacccgaaat
     2761 cttccagaaa atgtggatat cgaaaactcg gttatcacca ccaccttaac cgaaggtgct
     2821 attggctacg ccaaacttga tacccgcaag ggctatcaaa tcatgggggt tattcgcctg
     2881 gcagatggta gtcacccacc actggggatt agcgtaaaag ataaaaccag ccataaagaa
     2941 ttaggactgg ttgctgatgg cggctttgta tacctcaacg gcattcagga tgacagcaaa
     3001 cttactttac gctggggtga taaatcctgt tttattcaac cacccaatag cagcaactta
     3061 accaccggaa cggttatttt accgtgtatt agccaaaatt aattcaggat gaaactaatg
     3121 cgctacttaa acacaaagaa tctcattgca gcaggagtat tactcgcctg catgagcagc
     3181 attgcctggg gtgccatcat cccggaccgt acccgtatca taatgaatga gtcagataaa
     3241 ggggaagcat taaaacttac taatcaaagc aaaaatctac cctatctggc gcaaacatgg
     3301 atagaagata cgaaaggcaa taaatcacgt gactttattg tcacggttcc ccctatggta
     3361 cgtttaaatc ccagtgagca aattcaaata agaatgataa gtcaggaaaa aattgctcaa
     3421 ctgcctaatg acagagaaac gctcttctat tttaacgtgc gagaaatacc gccaaaaaca
     3481 gataaaaaaa atgtgatgca agtcactatg cagcacgcat tgaaactatt ctggcgacca
     3541 aaagctattg aattagaaga tgatggggta atgtcttacg aaaaagttga aatcatccgt
     3601 agaaatgatg gttcgatccg ctttaacaat aagatgcctt atcacgttac gctgggttat
     3661 atcggaacaa acggaatcac aatgttgcca caaacgcaaa gtttaatggt aaccccttat
     3721 agccatgcag atacgcagtt taaaaatgtt ccgtcagctt ttcaggttgg ttatatcaat
     3781 gattttggtg gattaagttt ttatgaaata aactgcccga cggttaataa ctcttgtaat
     3841 gtttccgtag ccaaacgaga taaataatat gcgctactta ttaattgtaa ttatatttat
     3901 tataggtttt agtgcattac ctgtatgggc aatggactgc tatgctgaac atgaaggggg
     3961 aaataccgta gtcattggtt acgtaccaag aatttctatc cccagtaatg gtaaaaaagg
     4021 tgataagatc tggcaaagca gtgagtattt tatgaatgtc ttctgtaata atgcattacc
     4081 tgccccatca cctggagaag aatacccatc agcctggact aatataatga tgtttttacc
     4141 aggcggtcag gacttttata atcagaactc ctatattttt ggcgtaacat acaatggagt
     4201 tgattatgat tcgactgccc ctaatgctat cgcagcacca gaatgcattg atataaaagg
     4261 agcgggtacc tttaataatc actataaaaa ccccgctgtc tgtagtggag gtcctgagcc
     4321 tcaattatca gtaacctttc cagctcgcgt acaactctat attaaattag caaaaaatgc
     4381 caatagagtt aataaaaacc tggtgttacc tgatgaatat atcgctctag agtttaaagg
     4441 tatgagtggc gcaggagcta tagaagtaga caaaaatttg accttcagga ttcgtggatt
     4501 aaataatatt catgtccttg actgctttgt taatgtcgca ctggaaccag ctgatggcgt
     4561 tgttgacttt ggtaaaataa attcccgaac aattaaaaat accagcgtta gtgagacgtt
     4621 tagtgtagtc atgacaaaag atcctggtgc ggcctgtact gagcaattca atattttagg
     4681 gagctttttc actacggata ttttgagtga ttatagccat ctggatatgg gtaatggact
     4741 gctattgaag atatttcata aagatggaac agcaacggaa tttaaccgct tctcacaatt
     4801 tgcttctttt tcatcgtcta gtgcaccttc ggtcaccgca ccattcaggg cagaacttag
     4861 tgcgaacccg gcagagactg ttgttgaggg accgtttagt aaagacgtaa tcctcaaaat
     4921 cacctat
//
LOCUS       DAZRUF010000260         4899 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZRUF010000260 DAZRUF010000000
VERSION     DAZRUF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.262"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            83..1999
                     /gene="mngA"
                     /locus_tag="ACHXOL_003760"
     CDS             83..1999
                     /gene="mngA"
                     /locus_tag="ACHXOL_003760"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR6810630.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            2017..4650
                     /gene="mngB"
                     /locus_tag="ACHXOL_003761"
     CDS             2017..4650
                     /gene="mngB"
                     /locus_tag="ACHXOL_003761"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR6810631.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            4675..>4899
                     /locus_tag="ACHXOL_003762"
     CDS             4675..>4899
                     /locus_tag="ACHXOL_003762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810632.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
BASE COUNT         1196 a         1144 c         1347 g         1212 t
ORIGIN      
        1 atcaagtgaa attgatcaca tgatggtatt gttttatcgg gcacactggc gcgactataa
       61 aaacgatcaa gtgaggatca tgatgaatct gacgactctg acccaccgcg atgcgttgtg
      121 tctgaatgcg cgctttacca gccgtgaaga ggccatccac gcgttgactc aacgtcttgc
      181 tgctctgggg aaaatttcca gtactgagca atttctggaa gaagtgtatc gccgtgaaag
      241 ccttggcccg accgccttag gtgaagggtt ggctgtgccg catggcaaaa ctgctgcggt
      301 aaaagaagcg gcgtttgcgg tcgccacact cagcgagccg cttcagtggg aaggcgttga
      361 tggcccggaa gcagttgatt tagtggtgct gctggcgatt ccccccaatg aagcgggtac
      421 tacgcatatg caactgctga cagcgctgac cacgcgcctt gcggatgatg agattcgggc
      481 gcgtatacag tcggcgacga cgcctgatga gttgctctcg gcgctggatg acaagggagg
      541 cacgcaacct tctgcctctt tttccaacgc gccaactatc gtctgcgtaa cggcctgtcc
      601 ggcgggtatt gctcacacct atatggctgc ggaatatctg gaaaaagccg gacgcaaact
      661 cggcgtaaat gtttacgttg aaaaacaagg cgctaacggc attgaagggc gtttaacggc
      721 ggatcaactc aatagtgcaa ccgcctgtat ttttgcggct gaagtcgcca tcaaggagag
      781 tgagcgtttt aatggcattc ccgcgctttc agtgcctgtt gccgagccga ttcgccatgc
      841 agaagcgttg atccaacaag cgcttaccct caagcgtagc gatgagacgc gtaccgtaca
      901 gcaagatacg caaccggtga aaagtgtcaa aacggagctg aaacaggcac tgttgagcgg
      961 aatctctttt gccgtaccgt tgattgtcgc ggggggcacg gtgctggcgg tcgcggtatt
     1021 actgtcgcaa atcttcgggc tacaagatct gtttaatgaa gaaaactcct ggctgtggat
     1081 gtaccgcaag ctgggcggcg ggctgctcgg aattttgatg gtaccggtgc tcgcggccta
     1141 taccgcctat tctctggcag ataaaccggc gttagcgcca ggctttgcgg ctggacttgc
     1201 cgccaacatg atcggctccg ggtttctcgg cgcggtcgtt ggcggattga tagccggtta
     1261 cttgatgcgc tgggtgaaaa atcacttgcg tcttagcagt aaattcaatg gattcctgac
     1321 tttttatctc tacccggtgc tcggtacgtt gggagcgggc agtctgatgc tgtttgtggt
     1381 gggagaacct gtcgcatgga tcaataactc gcttaccgcc tggctgaacg gtctgtcagg
     1441 aagtaacgcg ctgttgctgg gtgccattct cggttttatg tgttcctttg accttggagg
     1501 gccagtgaac aaagccgctt atgcattctg cctgggcgca atggcgaacg gcgtttacgg
     1561 cccgtatgcc attttcgcct ccgtcaaaat ggtttcggca tttaccgtaa ccgcttccac
     1621 gatgctcgca ccgcgcctgt ttaaagagtt tgaaattgag accgggaaat ccacctggct
     1681 gttagggctg gcaggtatta ccgaaggggc gatcccgatg gcgattgaag atccgctgcg
     1741 ggttattggt tcgtttgtgc tgggctctat ggtaacgggc gctattgtcg gtgcgatgaa
     1801 tatcggcctt tcgacacccg gtgccggcat tttctcgctc tttttacttc atgataatgg
     1861 cgcgggcggt gttatggcgg caattggctg gtttggtgcg gcattggtgg gggctgcaat
     1921 ctcgactgca attctcctga tctggcggcg tcacgcggtt aagcatggca actatctgac
     1981 tgatggcgta atgccataaa caaaaaggaa acgacgatga aagcggtatc tcgcgttcac
     2041 atcaccccgc atatgcattg ggatcgggag tggtatttca ccaccgaaga gtcacgcatt
     2101 ctgctggtca ataatatgga agagatcctg tgccgactgg aacaggacaa cgaatacaaa
     2161 tattacgtac tcgacgggca aacggcgatc ctcgaagatt atttcgcggt gaaaccggaa
     2221 aacaaagacc gcgtgaagaa acaggtagaa gccggtaagt tgattatcgg cccctggtat
     2281 acccagaccg ataccacgat tgtttctgcg gaatccatcg tccgtaatct gatgtacgga
     2341 atgcgtgact gcctcgcgtt tggcgagccg atgaaaatag gttatttacc ggattccttt
     2401 ggcatgtccg ggcaactgcc gcatatttac aatggatttg gcattacccg cactatgttc
     2461 tggcgcggat gttcggagcg ccacggtact gataaaaccg agtttttgtg gcaaagcagt
     2521 gacggtagcg aagtgacggc gcaggtgctg ccgctgggct acgccatcgg taagtactta
     2581 cctgccgacg aaaacggatt acgtaaacgc ctcgacagtt attttgacgt gctggaaaaa
     2641 gcgtctgtaa ccaaagagat tttgctgccg aatggtcatg accagatgcc attgcagcaa
     2701 aatatcttcg aagtgatgga taagctacgt gagatctacc ctcaacgtaa gtttgtgatg
     2761 agccgctttg aagaggtatt tgagaagatc gaagcgcagc gtgaaagtct ggccaccctg
     2821 aaaggtgaat ttatcgatgg caaatatatg cgcgtacatc gcaccatcgg ttccacgcgc
     2881 atggatatca aaattgccca cgcacgtatt gaaaataaga ttgttaatct gctggaaccg
     2941 ctggcaacgc tggcctggac gttgggtttt gaataccacc acggcttgct ggagaaaatg
     3001 tggaaagaga tcttaaaaaa tcatgcccac gacagtatcg gttgttgttg cagtgacaaa
     3061 gttcatcgcg aaattgtcgc ccgcttcgaa ctggctgaag acatggcgga taatctgctg
     3121 cgtttctaca tgcgcaaaat tgccgataac atgccacaga gtgatgccga caaactcgtg
     3181 ctgtttaacc tgatgccctg gccgcgggaa gaagttatta acaccactgt gcggctgcgc
     3241 gccagccagt ttaatctaca agacgatcgc ggtcagcctg taccgtattt tattcgccat
     3301 gcccgtgaga tcgatcctgg cctaatcgat cggcaaattg ttcattatgg caattatgat
     3361 ccctttatgg agtttgatat tcagatcaac cagattgtcc cttctatggg ctatcgcacg
     3421 ctttatattg aagcgaatca gcctggcaac gtgattgcgg caaaaagtga cgctgaaggg
     3481 attctggaaa atgctttctg gcagattgcg ctcaatgagg atggttctct gcaactggta
     3541 gataaagaca gcggtgtgcg ctatgaccgg gtattgcaaa ttgaagaaag ctctgatgat
     3601 ggtgatgaat atgactattc acccgcaaaa gaagagtggg taattaccgc agcgaacgcg
     3661 aaaccgcaat gcgatattat tcatgaagcc tggcagagca gggctgttat ccgctatgac
     3721 atggcagtgc cgctcaattt gtcagaacgc agcgcccggc aatccactgg cagagtaggg
     3781 ggggtgttgg ttgtcactct tagtcataac agcaggcgta ttgatgtgga tatcaacctt
     3841 gataaccagg ctgacgatca tcgccttcgt gtcctggtcc ctacaccttt taacactgac
     3901 agtgttctgg cagatacgca gtttggttcg ctaacgcgcc ccgtgaacga cagtgcaatg
     3961 aacaactggc agcaagaagg ctggaaagaa gcgccggttc cggtatggaa tatgctcaac
     4021 tatgttgcct tacaggaagg gcgtaacggc atggctgtct ttagcgaagg gttacgtgaa
     4081 tttgaagtca tcggtgaaga gaagaaaacc tttgccatta cgttgctgcg tggcgtgggc
     4141 ttactgggca aagaagatct gcttttaagg cctgggcggc cttcgggaat taaaatgcca
     4201 gtcccggact cacaactacg tggtctgctt tcttgtcgcc taagtttatt gagttatacc
     4261 ggtacgccaa ccgccgctgg tgtagctcag caggcgcgag catggctgac tccagtacag
     4321 tgttacaaca aaatcccatg ggatgcgatg aagctcaaca aagccggatt caacgtgccg
     4381 gaaagttata gtttgttgaa aatgccccca gtgggatgcc tgataagcgc acttaagaaa
     4441 gctgaagacc gacaagaagt gattttacgg ctgtttaatc cggctgaatc agcaacctgt
     4501 gatgcgactg ttgctttcag tcgcgaggtg atttcttgct cagaaacgat gatggatgaa
     4561 cacattacca ccgaggaaaa tcaaggttca aatctatcgg ggcctttttt acccggccag
     4621 tcacggacgt tcagttaccg gcttgcctga atagcaatca aaccgaagcc acatatgcgc
     4681 ggccagattg ttgacaaagg gcgctttgtt catgccggat acggcatgaa cgctttattc
     4741 ggtctacaaa agcaggcaaa ttcaatatat tgcagagatg atgtagacac tggcaagcgt
     4801 agcgcatcag gtaattttgc gtttatcttc actctcaagc cacgtatatg tggctttatt
     4861 tttaacaaaa taataaccct gggtgagtta attataata
//
LOCUS       DAZRUF010000261         4885 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZRUF010000261 DAZRUF010000000
VERSION     DAZRUF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.263"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            121..4083
                     /gene="putA"
                     /locus_tag="ACHXOL_003763"
     CDS             121..4083
                     /gene="putA"
                     /locus_tag="ACHXOL_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR6810633.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(4123..4761)
                     /gene="rutR"
                     /locus_tag="ACHXOL_003764"
     CDS             complement(4123..4761)
                     /gene="rutR"
                     /locus_tag="ACHXOL_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR6810634.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
BASE COUNT         1090 a         1332 c         1374 g         1089 t
ORIGIN      
        1 ttcattcatt tttaagcttg ctacgcaagt cacatttaac atggttgcac aaagttgcaa
       61 catcatggat atttcacgat aacgttaagt tgcacctttc agaacaacag gagtaatggc
      121 atgggaacca ccaccatggg ggttaagctg gacgacgcga cgcgtgagcg tattaagtct
      181 gccgcgacac gtatcgatcg cacaccacac tggttaatta agcaggcgat tttttcttat
      241 ctcgaacaac tggaaaacag cgatactctg ccggagctac ctgcgctgct ttctggcgcg
      301 gccaatgaga gcgatgaagc accgactccg gcagaggaac cacaccagcc attcctcgac
      361 tttgccgagc aaatattgcc ccagtcggtt tcccgcgccg cgatcaccgc ggcctatcgc
      421 cgcccggaaa ccgaagcggt ttctatgctg ctggaacaag cccgcctgcc gcagccagtt
      481 gctgaacagg cgcacaaact ggcgtatcaa ctggccgata aactgcgtaa tcaaaaaaat
      541 gccagtggtc gtgcaggaat ggtccagggg ttattgcagg agttttcgct gtcatcgcag
      601 gaaggcgtgg cgctgatgtg tctggcagaa gcgttgttgc gtattcccga caaagccacc
      661 cgcgacgcgt taattcgcga caaaatcagc aacggtaact ggcagtcaca cattggtcgt
      721 agcccgtcac tgtttgttaa tgccgctacc tgggggctgc tgtttactgg caaactggtt
      781 tccacccata acgaagccag cctctcccgc tcgctgaacc gcattatcgg taaaagcggt
      841 gaaccgctga tccgcaaagg tgtggatatg gcgatgcgcc tgatgggcga gcagttcgtc
      901 acgggtgaaa ccatcgcgga agcgttagcc aatgcccgca agctggaaga gaaaggtttc
      961 cgttactctt acgatatgct gggcgaagct gcactgactg ccgcagacgc acaggcgtat
     1021 atggtttcct atcagcaggc gattcacgcc attggtaaag cgtctaacgg tcgtggcatc
     1081 tatgaaggtc cgggcatttc aatcaaactg tcggcgctgc atccgcgcta cagccgcgcc
     1141 cagtatgacc gggtaatgga agagctttac ccgcgtctga aatcactcac cctgctggcg
     1201 cgtcagtatg atattggtat taacatcgat gccgaagagg ccgatcgtct ggagatctcc
     1261 ctcgatctgc tggaaaatct ctgtttcgag ccggaactgg caggctggaa cggcatcggt
     1321 tttgttattc aggcttatca aaaacgctgc ccgttggtga tcgattacct ggttgatctc
     1381 gccacccgca gccgtcgccg tctgatgatt cgcctggtga aaggcgcgta ctgggatagt
     1441 gaaatcaagc gtgcgcagat ggacggcctt gaaggctatc cggtttatac ccgcaaggtg
     1501 tataccgacg tttcttatct cgcctgtgcg aaaaagctgc tggcggtgcc gaatctaatc
     1561 tacccgcagt tcgcgacgca caacgcccat acgctggcag cgatttatca actggcaggt
     1621 cagaactact atccgggtca gtacgagttc cagtgcctgc atggtatggg cgagccactg
     1681 tacgagcagg tcaccgggaa agttgccgac ggtaaactta accgaccgtg tcgtatttat
     1741 gccccggttg gcacacatga aacgctgctg gcgtatctgg tgcgtcgcct gctggaaaac
     1801 ggtgctaaca cctcgtttgt taaccgtatt gccgacacct ctttgccact ggatgaactg
     1861 gtcgccgatc cggtcactgc tgtagaaaaa ctggcgcaac aggaagggca aactggatta
     1921 ccgcatccga aaattcccct gccgcgcgat ctttacggtc acgggcgcga caactcggca
     1981 gggctggatc tcgctaacga acaccgtctg gcctcgctct cctctgccct gctcaatagt
     2041 gcactgcaaa aatggcaggc cttgccaatg ctggaacaac cggtagcggc aggtgagatg
     2101 tcgcccgtta ttaaccctgc ggaaccgaaa gatattgtgg gctttgtgcg tgaagccacg
     2161 ccacgtgaag tagaacaggc gctggaaagt gcggttaata acgcgccaat ctggtttgcc
     2221 acgcctccgg ctgaacgcgc ggcgattttg catcgcgctg ccgtgctaat ggaaagccag
     2281 atgcagcaac tgattggtat tctggtgcgt gaagccggaa aaaccttcag taacgccatt
     2341 gccgaagtgc gcgaagcggt cgattttctc cactactacg ccggacaggt gcgggatgat
     2401 ttcgctaacg aaacccaccg tccattaggg cctgtggtgt gtatcagtcc gtggaacttc
     2461 ccgctggcta ttttcaccgg gcagatcgct gccgcactgg cggcaggtaa cagcgtgctg
     2521 gcaaaaccgg cagaacaaac gccgctgatt gccgcgcaag ggatcgccat tttgctggaa
     2581 gcgggtgtac cccctggcgt ggtgcaattg ctgccaggtc agggggaaac cgtgggcgcg
     2641 caactgacgg gtgatgatcg cgtgcgcggg gtgatgttta ccggttcaac cgaagtcgct
     2701 acgttactgc agcgcaatat cgccagccgc ctggacgctc agggtcgccc tattccgctc
     2761 atcgctgaaa ccggcggcat gaacgcgatg attgtcgatt cttcagcact gaccgaacaa
     2821 gtcgtcgtgg atgtgctggc ctcggcgttc gacagtgcgg gtcagcgttg ttcggcactg
     2881 cgcgtgctgt gcctgcaaga tgagattgcc gaccacacgt tgaaaatgct gcgcggtgca
     2941 atggccgaat gccggatggg taatccgggt cgcctgacca ccgatatcgg tccggtgatt
     3001 gatagcgaag cgaaagccaa tattgagcgc catattcaga ccatgcgtag caaaggccgt
     3061 ccggtgttcc aggcggtgcg ggaaaacagc gaagatgccc gtgaatggca aagcggcacc
     3121 tttgtcgccc cgacgctgat cgaactggat gactttgccg aattgcacaa agaggtcttt
     3181 ggtccggtgc tgcatgtggt gcgttacaac cgtaaccagc taccagagct gatcgagcag
     3241 attaacgctt ccggctatgg tctgacgctt ggtgtccata cgcgtattga tgaaaccatc
     3301 gcccaggtca ctggctcggc ccatgttggt aacctgtatg ttaaccgtaa tatggtgggc
     3361 gcagtggttg gtgtgcagcc gttcggcggc gaagggttgt ccggtaccgg gccgaaagca
     3421 ggcggtccgc tctatctcta ccgtctgctg gcgaatcgcc cggaaagtgc gctggcagtg
     3481 acgctcgcgc gtcaggatgc agagtatccg gtcgatgcac agttgaaagc cgcattgact
     3541 cagccgctaa atgcactgcg ggaatgggca gcaaatcgtc cagaattgca ggcgttatgt
     3601 acgcaatatg gcgagctggc gcaggcagga acacaacgat tgctgccggg gccgacgggt
     3661 gaacgcaaca cctggacgct gctgccgcgt gagcgcgtgt tgtgtattgc cgatgatgag
     3721 caggatgcgc tgactcagct cgccgccgtg ctggcggtgg gcagccaggt actgtggccg
     3781 gatgacgcgc tgcatcgtca gctagtaaag gcattgccat cggcagtcag cgaacgtatt
     3841 caactggcga aagcggaaaa tataaccgct caaccctttg atgcggtgat cttccacggt
     3901 gattcggatc agcttcgcgc attgtgtgaa gccgttgctg cgcgggatgg cgcaattgtt
     3961 tcggtgcagg gttttgcccg tggcgaaagc aatatccttc tggaacggct gtatatcgaa
     4021 cgttcgctga gtgtgaatac cgctgccgct ggcggtaacg ccagtttaat gacgatcggt
     4081 taaacaggta gccggaggat gttacaacct cctccggcat ctttaacgtg gtcgaatccc
     4141 ctcaataata atccgctgca cgttttcaac cgtttgattg aaaaatatct catcgcgcaa
     4201 cgtcgcgcct gtcaccgcct ccacctgagg ggcgaaatcg gcgtaatgtt gagtggaagc
     4261 ccaaatcatg aaaatcaaat gctgcggatc aatcggcgcg agtttgccgc ttttgaccca
     4321 accggcaatc agcgcagatt tctcatcaat taatgccttc aaatcgcccg tcagttcatc
     4381 cattaacagc ggcgcgcctg ccagcatctc catacagaac aggcgcgaag cctgcggata
     4441 atcgcgggag acttccagct tcagacggat gtactctttt atcgccgcca gcggagcgaa
     4501 atcttcacga aacgctttta acggtgccag ccagatatcg agaatctgcc gcagcacggc
     4561 aatatacagc gcctcttttg acggaaagta atacagcaga ttggtttttg aaacacccgc
     4621 caactctgcg atctgctcca gccttgtgcc gtgaaaaccg aattgtgaaa aagtgtccag
     4681 tgctgcgcta agaatcgctt ttttcttcgc gcttactgcg cgcgaacgtt tacccgttgt
     4741 tttcactgcg ccttgcgtca tgcgctctcc cctcttgtag attggcaaca ggatagcaaa
     4801 acccccgccc ggcctcgacc ttctgcactc tcatcgcgca cagatgcatg ttttatgtgc
     4861 aactgttttg accgtttagt ccact
//
LOCUS       DAZRUF010000262         4855 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZRUF010000262 DAZRUF010000000
VERSION     DAZRUF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.264"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            192..905
                     /gene="ydiV"
                     /locus_tag="ACHXOL_003765"
     CDS             192..905
                     /gene="ydiV"
                     /locus_tag="ACHXOL_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR6810635.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            968..2404
                     /gene="selO"
                     /locus_tag="ACHXOL_003766"
     CDS             968..2404
                     /gene="selO"
                     /locus_tag="ACHXOL_003766"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR6810636.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(2408..2599)
                     /gene="hemP"
                     /locus_tag="ACHXOL_003767"
     CDS             complement(2408..2599)
                     /gene="hemP"
                     /locus_tag="ACHXOL_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR6810637.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2731..3777)
                     /gene="aroH"
                     /locus_tag="ACHXOL_003768"
     CDS             complement(2731..3777)
                     /gene="aroH"
                     /locus_tag="ACHXOL_003768"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR6810638.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3934..4767)
                     /gene="ppsR"
                     /locus_tag="ACHXOL_003769"
     CDS             complement(3934..4767)
                     /gene="ppsR"
                     /locus_tag="ACHXOL_003769"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR6810639.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         1201 a         1131 c         1252 g         1271 t
ORIGIN      
        1 gtagtatttt gcagacaaaa attccgtaaa tggattattc gtttataatc aggataatct
       61 aaaaaaagga cccctgaaaa agtggttaac atggaacaaa tgaaagttac tccttctgta
      121 attgttatgt cttatggcct catccctttt agccggatac tgaaaaacat ccttcgagag
      181 ggacggttac catgaagatt tttttggaga atctttatca ttctgattgc tatttcctgc
      241 cgataagaga taaccagcaa gtccttgtcg gcgttgagtt aattacccat ttctccagtg
      301 aagatggcac ggtgcgtatc ccgacgagtc gggttatagc gcaacttacc gaggagcaac
      361 attggcaatt attttcagag cagttggaat tactaaaatc atgccaacat ttttttattc
      421 aacataaact ctttgcctgg cttaatttaa cgccacaagt ggctacatta ttattagaga
      481 gggataatta cgcaggcgaa ctattaaaat atccgtttat tgaactgttg attaatgaga
      541 attacccgca tctcaatgaa ggaaaagata atcgcggtct tttatcctta tcgcaagtgt
      601 atccccttgt gttaggtaat ttgggagcag gcaatagcac aatgaaagcg gtttttgatg
      661 gtcttttcac ccgcgtcatg ctggataaaa gttttattca gcaacaaatc acgcaccgct
      721 ccttcgaacc gtttattcgt gcgatccagg cgcaaatttc cccttgttgc aattgcatca
      781 ttgcgggagg cattgatacg gcagaaatat tggcacagat tacccccttc gatttccatg
      841 ctctccaggg atgcctgtgg cctgccgttc ccataaatca gataacgacg ttggttcagc
      901 gataaccctt ctgtttgctg gtgtttaaga cgagagtaac cgtctacact atcaaacagg
      961 aggatctatg accctgtctt ttgttacccg ctggcgcgat gaattgccag aaacctatac
     1021 agcactttcc cctacgcctt taaataatgc ccggctgatt tggcataata ccgaactggc
     1081 taacacgctg agtattccat cgtcgctgtt taaaaatggc gcaggtgtct ggggcggcga
     1141 agccctactg cctggtatgt caccactggc gcaggtttac agtggtcatc agttcggcgt
     1201 ctgggcgggc caactgggtg atgggcgcgg cattttactc ggcgaacaac tgcttgctga
     1261 tggcactaca atggactggc atctgaaagg tgctggcctg acgccttatt cgcgaatggg
     1321 tgatggacgg gcggttttac gttcgacgat acgagaaagt ctcgccagtg aggcgatgca
     1381 ttatctgggc attccgacga cccgcgcgtt aagtatcgtc accagcgatt cgccagtgta
     1441 tcgggaaacg gcggagccag gcgcgatgct gatgcgtgtg gcaccaagtc atctgcgctt
     1501 tggtcatttc gaacattttt actaccgccg cgagtcggaa aaggttcgtc agttagctga
     1561 ctttgctatt cgtcattact ggtcacatct tgaagatgat gaggacaaat accgtctctg
     1621 gtttagcgat gttgtcgcac gtaccgcgtc gttaattgcc caatggcaga cggtcggctt
     1681 tgcacatggg gtgatgaata ccgacaacat gtcgctgctg ggactgacgc ttgattacgg
     1741 gccgtttggt tttcttgatg attacgaacc cggttttatt tgtaatcact cggatcatca
     1801 agggcgttat agttttgata atcaacctgc tgtcgcgttg tggaatttac agcgtctggc
     1861 gcagacattg tcaccatttg ttgccgtaga tgccctgaat gaggccctgg acagctatca
     1921 gcaggttttg ttgacgcatt atggacaacg gatgcggcag aaactgggct tcatcacgga
     1981 gcaaaaagag gataacgcgc tactgaatga attattcagt ctgatggcgc gagagcgcag
     2041 cgattatacc cgcacattcc gcatgctgag tctgaccgag cagcacagca cggcgtcacc
     2101 gctacgtgat gagtttattg atcgtgcggc atttgatggc tggtttgccc gttatcgggg
     2161 gcgtttgcaa caagacgagg ttagcgatag tgagcgtcag cagttgatgc aaagcgttaa
     2221 ccccgctctg gtattgcgca actggttggc gcaacgggcg attgaagcgg cagaaaaggg
     2281 tgatatgacg gaattgcacc gcctgcatgg ggcgttgcga aatcctttca gcgatagaga
     2341 tgatgactat gtcagtcgtc cacctgactg gggtaaacgg ctggaagtca gctgctcgag
     2401 ttaaaggcta cttagttaac agcagcttgc cagcctgagt tttacggagc agatattctt
     2461 gcccgtcatg atcgataatc agtttgccat ccggacctaa cagtgtctgg ctggtaattc
     2521 gccggatggg ctgcggggaa gcggccttgt gattggcgtc cgtttcaggc gtgagttttc
     2581 tgctatccgt ataacgcata aagattaata ataattgata taacaatgat tcttattatc
     2641 aatattatgg caggccagag caagcagtaa tttgttgaga agacaacaag ttgcgcagaa
     2701 atgcccattc cgtcaggaat gggcacgcat tcagaagcgg gtatctaccg cagaggcgag
     2761 tttttcgacc aggcgttcgg tatcttccca gcccagacac gggtcggtaa tggattgacc
     2821 gtaagtgagc ggctgactgc cgacgatttt ttgcgttcct tcgagcagga aactttccgc
     2881 cataattcca gcaatcgccg tagagccatt gcggatttgc tgacaaatat cctcacaaac
     2941 ttctaactgg cgacggtgct gcttctggca gttaccgtgg ctgaaatcca ccaccagatg
     3001 ttcaggtaaa tcaaactcgt gcagcgtatc gcaggctgcg gcgatatcat cggcatgata
     3061 attcggtttt ttgccgccac gcataataat gtggccatac gggttgccgc tggtctgata
     3121 gatggtcatc tgaccatttt tgtctggcga gaggaacata tggctggcgc gggctgcgcg
     3181 gatagcatcc acagcaatcc gcgtattgcc atcggtacca tttttaaaac ctaccggaca
     3241 ggagagtgcc gaagccattt cacggtggat ctgactttcg gtagtacgtg cgccaatcgc
     3301 gccccaactg attaaatcag caataaactg accggtcacc atatcgagga actcagtcgc
     3361 ggttgggacg cccagctcat ttacctgtaa aagtaatttg cgcgccagct ccagaccgtg
     3421 atttacccga tagctgccgt ttaaatctgg atcggagatt agtcctttcc agccgacaac
     3481 agttcgtggt ttttcaaaat aggtgcgcat tacgatttcc agccgtgact ggtactggtt
     3541 gcgcagcgac tgcagacggg gggcgtactc cattgcagcg gtgagatcgt ggatcgagca
     3601 ggggccaata atgaccaaca gtcgcttatc ttcaccattg agtatttttt caattctgcg
     3661 gcgggagtcg gtgacatggg tggcggcgcc aggcgttacg ggataccgta gcgcgagttc
     3721 ggcgggcgtt accaggctct caatacgcgc agtacggagt tcgtcagttc tgttcattac
     3781 aagtctcaga aatttgttgc ttctacggca ggactgccgg gaagtgatgc gcatcacgat
     3841 aaaccaatcg ctgctgattt caagtgcgag gtgtgtcaca cctcgccggg tggttagcat
     3901 cataacaaaa aaataagcta atgcactagt tctctagtac attcggcgac taaggcccat
     3961 gatatcgagg atcttggtgg caatctcttc taccgaataa ttggtactgt taatccacgg
     4021 gatctgattt ttacggtaca acgcttccac ttccgcgact tccatcctgc actgacgaag
     4081 cgaggcatag cgactgttct cccgacgttc ctcgcgaatc gccgccagac gttccgggtc
     4141 gatagtcagg ccgaacaatt tatgctgaag cggtttgagc gacgcgggta gcaccagatt
     4201 atccatatcg tcggcaataa aggggtagtt tgccgcgcgg ataccaaatt gcattgccag
     4261 ataaagactg gtgggggttt taccacagcg agaaacaccg agcaggatca cctgagcctg
     4321 atccagattg cgcaacgaaa tgccgtcatc gtgggcgagg gtgtaatcaa tcgccgcaat
     4381 gcgcgcatca tatttattga gattattagg gttaaggcca tgggtacgat gagcaatcgg
     4441 cgttggatcc agtttcatct cttgttgtag cggggcaacc agcgcctgaa cgatatcctg
     4501 gcaaaagcct tcactttgca agatgatggc gcgaatctcc ggcaacacga tggagtagaa
     4561 gaccagcggg cgcacacctg tctggtgata aattgcgtca atctgatcct tcactgcccg
     4621 tgcacggctc tcattttcga caaacggcag cgtaatgctg ctgatagtga cgggaaattg
     4681 tgacattact gcgtgtccta atacctccgc agttattgcc gtaccatcag aaatataaaa
     4741 aacgtggcga tcaacagcat tatccatttt gttcttcccg tgatgcagac ataattctat
     4801 gaaagcataa attaaaaacg cacagaagcg taggacgtta tgtctggttt ataaa
//
LOCUS       DAZRUF010000263         4841 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZRUF010000263 DAZRUF010000000
VERSION     DAZRUF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.265"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..424
                     /locus_tag="ACHXOL_003770"
                     /pseudo
     CDS             1..424
                     /locus_tag="ACHXOL_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(466..918)
                     /locus_tag="ACHXOL_003771"
                     /pseudo
     CDS             complement(466..918)
                     /locus_tag="ACHXOL_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            982..1128
                     /locus_tag="ACHXOL_003772"
                     /pseudo
     CDS             982..1128
                     /locus_tag="ACHXOL_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1128..2285)
                     /gene="intS"
                     /locus_tag="ACHXOL_003773"
     CDS             complement(1128..2285)
                     /gene="intS"
                     /locus_tag="ACHXOL_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR6810640.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2447..2521)
                     /locus_tag="ACHXOL_003774"
     tRNA            complement(2447..2521)
                     /locus_tag="ACHXOL_003774"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2486..2488),aa:Arg,seq:cct)
     gene            complement(2597..3529)
                     /locus_tag="ACHXOL_003775"
     CDS             complement(2597..3529)
                     /locus_tag="ACHXOL_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR6810641.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3823..4578
                     /gene="mlaA"
                     /locus_tag="ACHXOL_003776"
     CDS             3823..4578
                     /gene="mlaA"
                     /locus_tag="ACHXOL_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR6810642.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4760..>4841)
                     /locus_tag="ACHXOL_003777"
     CDS             complement(4760..>4841)
                     /locus_tag="ACHXOL_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810643.1"
                     /translation="RLQNRQAKYQNEINQLSAPGVNFDDI"
BASE COUNT         1182 a         1148 c         1122 g         1389 t
ORIGIN      
        1 ttgatgagtc tattgctttt acatgtctct ctgcattttc ttctgaaaaa caatttggag
       61 tatttctgtt tggatgtttg aaaagcacag ggctaaagtt aaaacgatta ttcatacgga
      121 tttatctgca ccatggcgtc ttaaggatat atcatcaaga ttatatctca gcgaaagttt
      181 actaaagatg aaattgaaag aagagggggt atcattcagt aagatcatac ttgatgagag
      241 gatgcaaatg gctgaatatt tactcagcac tcgttgttat cctattagta aagtagctaa
      301 ggtctgtggt tatgccagtg tctcatactt tacttatgta tttagacgtt attttggtgt
      361 ttctccaagt caatactctc agaggagttc agaaagtaaa attcttactc accagggaat
      421 ctgatcattg ttcttgcccc cttatttcca gacagggggt gtatcttaag ttaacgttac
      481 ccgctgacgt cgatattctc gcggagagcg ataacccagc gcactgtgcg gatggttttc
      541 attgtaatgt tcgatcgcca ctgcaagatt atgcaatgcc gttcttacat tcggtttcgg
      601 catgaacgcg atgtagtctt ccttcatcgt tttcacgaac ctttctgcca ttccattact
      661 ctgaggactg ctgattgccg ttgtgcaggg ctccagattc aactctctgg cgaactgccg
      721 tgtttcatgc gctctatacg ctgaaccgtt atctgtcagc cactggatgg actgttccgg
      781 tactttatcg ccaaagcgtt tttctatcgc tcctaacatg acatcctgca ctgtcgcttt
      841 atcgtaacct cccgtgcttg cggcccagtc tatggcttca cgatcgcaac agtccagggc
      901 gaacgtaaca cgcagttttg aatcgccacg ggtttaacag acacctcaga gtcatttaag
      961 atggcttaaa gagaggtgcc catgagcggt aagcgttatc ccgaagagtt taaaactgaa
     1021 gcagtcaaac aggttgttga tcgcggttat tctgttgcca gcgttgcaac acgtctcgat
     1081 atcactaccc acagccttta tgcctggata aagaagtacg gtccggatta ctccactttt
     1141 tcatcaagcc aatccgccca ccactgcatc atttctctgc gcttatcgag atactgagca
     1201 tggttgtaaa taccgcgcac agatccgccg ttggcatgtg ccagctgcac ttcaatagcg
     1261 tcagcaggcc attcgtgctc gttcataatc gtgctgaatt catgcctgaa tccgtgaccg
     1321 ctttccagac cttcatagcc gatttgtttg atcacaagca ataccgcgtt ctcgcagatt
     1381 ggcttcttct tatcgttgcg cccggcaaaa acaaactctg agactggttt ggtgattgag
     1441 cttagcgtag tgagaagttc aaccacctgg tctgacatcg ggaccacatg aattttgcgt
     1501 cccttcatca cactggcgtc gatggtgata atcctgtttt caaaatcgac gttcttccat
     1561 agcatggaac gaagctcttt tgttcttagg gctgtgtagc gtaaaacttt ggtcgcaatg
     1621 agcgatacga tacttcctga aaatgttgcc agtgctttgt tgaatgcagg catctggtct
     1681 gctggaagga acgggaagtt cttcttgcgg tatcctttca tggcgtctgc aaggtcaggt
     1741 gccgggttat atttagccct tccggtgaca atagcgtaac gaaaaacctc gccgcatctt
     1801 ctgcgggctt tgttggctcg ctccattgca ccgcgatctt caaatctgcg gattacttcc
     1861 agcagttgca tcggctcaat atcctgaatc tcaagaccgc cgatgatggg taaaatgtcg
     1921 tcatcaaaca ttttggcaag ttcagttgca tagcctactg accagacttg cttcttgtgc
     1981 tcgtaccatt ccttgtaaat cgcactaaat gaattgttgt tagacgaagc ctttttcgcc
     2041 tttaccggaa cgatgccaac cgagatgtct ttcctcgcag tccatgcctt atcccttgct
     2101 tcctgcaaag tcataagcgg atattttccg acggtcagga ttttctcctt accgtcaatc
     2161 ttgtagcgaa gctgccatac ctttttcccg gatacaggaa cataaaggta caggccatta
     2221 ccatcgagaa ggcggtatgg tttttctttc ggctttgctg cttcaatctg cttaacggtg
     2281 agcatgggta aaaatccggt gggtaaaatt attttatcca ctttttaccc gtcatggagt
     2341 gcggctgtca acgatctgac gcgaaccatg acgaaccgtg aatatacgga aggcttgata
     2401 ttcaggggat tttgcggact ggtacggatg ggagcgaact gataaatggt gtcccctgca
     2461 ggaatcgaac ctgcaattag cccttaggag gggctcgtta tatccattta actaagagga
     2521 caatgcggca tgagtatacc cgctaatgga gtgcggggta agtacgctgc cgcttgattg
     2581 cttaaaccct cgccatttat gccgggtttt tatcattttt cttaatgttt tccgcacgtt
     2641 ctgctttttg gcgtgcttct gctttacgct tgttgctcat gtcgttacga atctgtgcat
     2701 gactcattaa cgcgaagata aaggtgccgc cgcagatgtt ccccgctaaa gtaggtagtg
     2761 cgaagggcca gatgaaatcg ctccagtgca gcgtaccgtt aaacaccaga tagaggattt
     2821 caacagaacc gaccacgata tgggtggtgt cacctagggc aataagccag gtcatcaata
     2881 taatcaccac aatctttgcc gcacctgctg caggaaacat ccataccata gtggcgatca
     2941 gccagccgga aatgatcgcg ttggcaaaca tctcgctggg ggtgttcttc atcacatcca
     3001 tgccgatttt gacaaatgca tcgcgagttt cttcattgaa gataggcata tattcaaatg
     3061 cccacgcagc aataccagtc ccgagaatat tacccagcag cacgactccc cataaccgca
     3121 taagtaagcc aacgttgctc attgtcggtt tttgcatgac gggtagtacc gcagtcacgg
     3181 tgttttcggt aaataattgc tggcgggcca ttatgacgat aataaaacca aaggtataac
     3241 cgagattctc cagtaagaag ctgcccggca caccttccag ttcgacatga aatatccctt
     3301 ttgccagtaa cgaagcgccc atcgacagac ccgccgcaat ggctgaccac agtagcgcca
     3361 ttgcgtcgcg ttccagctct ttttcaccat cctggcggat atgctcatga attgccatcg
     3421 cccgggaggg gagtcggtct tcatctattt ctattttttt gccgcgctct ttttcttcgc
     3481 tctcaacttc aatttcgtcg ctgtgttgat caattttgtc gttgtccatg gtctcttctc
     3541 ttgaattagc acgtatagct aaagcgtagc ggcttttttg ctcgcaactg gcgggagtta
     3601 ctctgaaaat gtagaaaagg ctgcgtttgc ctttttctgt ttctatagaa tcaagtagcc
     3661 tacagggcgg cgattaccag gctatgatca aatcagcaaa tcagggcgtc tggacatcag
     3721 ttgacgtgct gttacaatcg cccacaccta aacaggcgga tacggtatcg ttccgtcatg
     3781 gatggcaaac tgcataagcc ataaaaaaac agggagacat ttatgaagct tcgcctgtcg
     3841 gcgcttgctc tgggaactac gcttctggtg gggtgtgcga gttccggtac agatcagcaa
     3901 gggcgttctg acccgttaga agggttcaac cgcaccatgt acaacttcaa cttcaacgta
     3961 ttagacccgt atattgttcg accggtcgct gtcgcctggc gtgattatgt tccgcaaccg
     4021 gcgcgtaatg gtttgagcaa ctttactggc aaccttgaag aacctgcggt gatggttaac
     4081 tacttcttgc agggcgaccc ttatcagggg atggtccact ttacccgctt tttcctgaac
     4141 accattttgg ggatgggcgg ctttattgat gttgcaggga tggcgaaccc gaaactgcaa
     4201 cggactgaac ctcaccgctt cggtagtacg cttggtcatt atggcgtggg ttatgggcct
     4261 tacgttcagt taccgttcta cggtagcttc acgctgcgtg atgacggtgg tgatatggcg
     4321 gatggtcttt acccggttct ttcctggctg acctggccga tgtctgtggg taaatggacg
     4381 cttgaaggga tcgaaacccg cgctcagctg ctggattccg atggtctgct gcgtcagtcg
     4441 tccgatcctt atattatggt gcgcgaagcg tacttccagc gtcatgattt catcgctaat
     4501 ggcggcgaac tcaaaccgca ggaaaatccg aacgcacaag cgattcagga tgatttaaaa
     4561 gatattgatt ctgaataaga aacaaataaa aaaggtgagt cgcaaaactc accttttttg
     4621 ttgttatccc tcaattacca caagtaaaag gagataaacc gacaatgacc cggacgtaat
     4681 gatgtaaaaa tactgatgtt cgtcactgac tatttccgat attcaggcgt tgaaaaatca
     4741 aaaaactgga aaaataattt taaatatcat caaaattaac acctggcgca gatagttgat
     4801 taatttcatt ttgatatttc gcctggcgat tttgaagtct t
//
LOCUS       DAZRUF010000264         4834 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZRUF010000264 DAZRUF010000000
VERSION     DAZRUF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.266"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(108..551)
                     /locus_tag="ACHXOL_003778"
     CDS             complement(108..551)
                     /locus_tag="ACHXOL_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR6810644.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            708..1637
                     /gene="metA"
                     /locus_tag="ACHXOL_003779"
     CDS             708..1637
                     /gene="metA"
                     /locus_tag="ACHXOL_003779"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR6810645.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1906..3507
                     /gene="aceB"
                     /locus_tag="ACHXOL_003780"
                     /pseudo
     CDS             1906..3507
                     /gene="aceB"
                     /locus_tag="ACHXOL_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3537..4828
                     /gene="aceA"
                     /locus_tag="ACHXOL_003781"
                     /pseudo
     CDS             3537..4828
                     /gene="aceA"
                     /locus_tag="ACHXOL_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1205 a         1273 c         1225 g         1131 t
ORIGIN      
        1 gcagaaggcc atcctgacgg atggcctttt tgcattggcg cagaaaaaaa tgccgggtgc
       61 gacgctggcg cgtcttctcc agcctgggca ggcacggcag gggcagctta ttcctccaca
      121 tacgccagat tcagcaacgg atacggtttc cccaaatcgt ccacctcaga gcgtcccgta
      181 accttaaaac ccaccttctt atagaaccca accgcctgct cattttgctc attaacgttg
      241 gttgtcagtt ccggtgccat cgagagcgca tgctccacca gcatccgacc cacgccgcag
      301 ccgcacacat caggatcgat aaacagcgca tccatatgct gcccacttaa caacataaac
      361 ccaaccggct gatcccgttc attaaccgcg acccacaacg gcgcttccgg caggaaggaa
      421 cgaactagca cctccagctc ggcccgatac tctgctgata aaaaatcgtg tgtggcatcg
      481 acagaacgac accaaatcgc aacgagttcc tccccttcct catgccgtga gcggcgaata
      541 ctaataacca ttttctctcc ttttcgtcat tcttatattc taacgtagcc ttttccttga
      601 aactttctca ccttcaacat gcaggctcga cattggcaaa ttttctggtt atcttcagct
      661 atctggatgt ctaaacgtat aagcgtatgt agtgaggtaa tcaggttatg ccgattcgtg
      721 tgccggacga gctacccgcc gtcaatttct tgcgtgaaga aaacgtcttt gtgatgacaa
      781 cttctcgtgc gtctggtcag gaaattcgtc cacttaaggt tctgatcctt aacctgatgc
      841 cgaagaagat tgaaactgaa aatcagtttc tgcgcctgct ttcaaactca cctttgcagg
      901 tcgatattca gctgttgcgc atcgattccc gtgaatcgcg caacacgccc gcagagcatc
      961 tgaacaactt ctactgtaac tttgaagata ttcaggaaca aaactttgac ggtctgatag
     1021 ttaccggcgc gccgctgggc ctagtggagt ttaatgatgt cgcttactgg ccgcagatca
     1081 aacaggtgct ggagtggtcg aaagatcacg tcacctcgac gctgtttgtc tgctgggcgg
     1141 tacaggccgc gctcaatatc ctctacggca ttcctaagca aactcgcacc gacaaactct
     1201 ctggcgttta cgagcatcat attctccatc ctcatgcgct tctgacgcgt ggctttgatg
     1261 attcattcct ggcaccgcat tcgcgctatg ctgactttcc ggcagcgttg attcgtgatt
     1321 acaccgatct ggaaattctg gcagagacgg aagaagggga tgcatatctg tttgccagta
     1381 aagataagcg cattgccttt gtgacgggcc atcccgaata tgatgcgcaa acgctggcgc
     1441 aggaatattt ccgcgatgtg gaagccggac tagacccgga tgtaccgtat aactatttcc
     1501 cgcacaatga tccgcaaaac aaaccgcgcg cgagctggcg tagtcacggt aatttgctgt
     1561 ttaccaactg gctcaactat tacgtctacc agatcacgcc atacgatcta cggcacatga
     1621 atccaacgct ggattaatct tctgtgatag tcgatcgtta agcgattcag caccttacct
     1681 caggcacctt cgggtgcctt ttttatttcc gaaacgcacc tcagcaagtg aataaatttt
     1741 attcatattg ttatcaataa gttatcaagt atttttaatt aaaatggaaa ttgtttttga
     1801 ttttgcattt taaatgagta gtcttagttg tgctgaacga aaagcgcaca acgatccttc
     1861 gttcacagtg gggaagtttt cggatccatg acgaggagct gcacgatgac tgaacaggca
     1921 acaacaaccg atgaactggc tttcacaagg ccgtatggcg agcaggagaa gcaaattctt
     1981 actgccgaag cggtagaatt tctgactgag ctggtgacgc attttacgcc acaacgcaat
     2041 aaacttctgg cagcgcgcat tcagcagcag caagatattg ataacggaac gttgcctgat
     2101 tttatttcgg aaacagcttc cattcgcgat gctgattgga aaattcgcgg gattcctgcg
     2161 gacttagaag accgccgcgt agagataact ggcccggtag agcgcaagat ggtgatcaac
     2221 gcgctcaacg ccaatgtgaa agtctttatg gccgatttcg aagattcact ggccccggac
     2281 tggaacaaag tgatcgacgg gcaaattaac ctgcgtgatg cagttaacgg caccatcagc
     2341 tataccaatg aagcaggcaa aatttatcag ctcaagccca atccagcggt tttgatttgt
     2401 cgggtacgcg gtctacactt gccggaaaaa catgtcacct ggcgtggtga ggcaatcccc
     2461 ggtagcctgt ttgattttgc gctctatttc ttccacaact atcaggcact gttggcaaag
     2521 ggcagcggtc cctatttcta tctgccgaaa acccagtcct ggcaggaagc ggcctggtgg
     2581 agcgaagtct tcagctatgc agaagatcgc tttaatctgc cgcgcggcgc catcaaggcg
     2641 acgttgctga ttgaaacgct gcccgccgtg ttccagatgg acgaaatcct tcacgcgctg
     2701 cgcgaccata ttgttggtct gaactgcggt cgctgggatt acatcttcag ctatatcaaa
     2761 acgttgaaaa actatcccga tcgtgtcctg ccagacagac aggccgtgac gatggataaa
     2821 ccattcctga atgcttactc acgcctgctg attaaaacct gccataaacg cggtgctttt
     2881 gcgatgggcg gcatggcagc gtttattccg agcaaagatg aagagcgtaa taaccaggtg
     2941 ctcaacaaag taaaagcgga taaagcgctg gaagtcaata acggtcacga tggcacatgg
     3001 atcgctcacc caggccttgc ggacacggca atggcggtat tcaacgacat tctcggctcc
     3061 cgtaaaaatc agcttgaagt attgcgcgaa caagacgcgc cgattactgc cgatcagctg
     3121 ctggcacctt gtgacggtga acgcaccgaa gaaggtatgc gcgccaacat tcgcgtggct
     3181 gtgcagtaca tcgaagcgtg gatctccggc aacggctgcg tgccgattta tggcctgatg
     3241 gaagatgcgg cgacggctga aatttcccgt acctcgatct ggcagtggat ccatcatcaa
     3301 aaaacgttga gcaatggcaa accggtgacc aaagccttgt tccgctagat gctgggcgaa
     3361 gagatgaaag tcattgccag cgaactgggc gaagaacgtt tctcccaggg ccgttttgac
     3421 gatgccgcac gcttgatgga acagatcacc acttccgatg agttaattga tttcctgacc
     3481 ctgccaggct accgcctgtt agcgtaaacc accacataac tatggagcat ctgcacatga
     3541 aaacccgtac acaacaaatt gaagaattac agaaagagtg gactcaaccg cgttgggaag
     3601 gcattactcg cccatacagt gcggaagatg tggtgaaatt acgcggttca gtcaatcctg
     3661 aatgcacgct ggcgcaactg ggcgcagcga aaatgtggcg tctgctgcac ggtgagtcga
     3721 aaaaaggcta catcaacagc ctcggtgcac tgactggcgg tcaggcgctg caacaggcga
     3781 aagcgggtat tgaagcagtc tatctgtcgg gatggcaggt agcggcggac gctaacctgg
     3841 cggccagcat gtatccggat cagtcgctct atccggcaaa ctcggtgcca gctgtggtgg
     3901 agcggatcaa caacaccttc cgtcgtgccg atcagatcca atggtccgcg ggcattgagc
     3961 cgggcgatcc gcgctatgtc gattacttcc tgccgatcgt tgccgatgcg gaagccggtt
     4021 ttggcggtgt cctgaatgcc tttgaactga tgaaagcgat gattgaagcc ggtgcagcgg
     4081 cagttcactt cgaagatcag ctggcgtcag tgaagaaatg cggtcacatg ggcggcaaag
     4141 ttttggtgcc aactcaagaa gctattcaga aactggtcgc ggcgcgtctg gcagctgacg
     4201 tgacgggcgt tccaacccta ctggttgctc gtaccgatgc cgatgcagcg gatctgatca
     4261 cctccgattg cgacccgtat gacagcgaat ttattaccgg cgagcgtacc agtgaaggct
     4321 tcttccgtac tcatgcgggc attgagcaag cgatcagccg tggcctggcg tatgcgccat
     4381 atgctgacct ggtctggtgt gaaacctcca cgccggatct ggaactggcg cgtcgctttg
     4441 cacaagctat ccacgcgaaa tatccgggca aactgctggc ttataactgc tcgccgtcgt
     4501 tcaactggca gaaaaacctc gacgacaaaa ctattgccag cttccagcag cagctgtcgg
     4561 atatgggcta caagttccag ttcatcaccc tggcaggtat ccacagcatg tggttcaaca
     4621 tgtttgacct ggcaaacgcc tatgcccagg gcgagggtat gaagcactac gttgagaaag
     4681 tgcagcagcc ggaatttgcc gctatacctt cgtatctcac cagcaggaag tgggtacagg
     4741 ttacttcgat aaagtgacga ctattattca gggcggcacg tcttcagtca ccgcgctgac
     4801 cggctccact gaagaatcgc agttctaagc aacc
//
LOCUS       DAZRUF010000265         4813 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZRUF010000265 DAZRUF010000000
VERSION     DAZRUF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.267"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(54..2123)
                     /gene="glyS"
                     /locus_tag="ACHXOL_003782"
     CDS             complement(54..2123)
                     /gene="glyS"
                     /locus_tag="ACHXOL_003782"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR6810646.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2133..3044)
                     /gene="glyQ"
                     /locus_tag="ACHXOL_003783"
     CDS             complement(2133..3044)
                     /gene="glyQ"
                     /locus_tag="ACHXOL_003783"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR6810647.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3139..3438)
                     /locus_tag="ACHXOL_003784"
     CDS             complement(3139..3438)
                     /locus_tag="ACHXOL_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR6810648.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3613..4608
                     /gene="wecH"
                     /locus_tag="ACHXOL_003785"
     CDS             3613..4608
                     /gene="wecH"
                     /locus_tag="ACHXOL_003785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR6810649.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRIRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4650..>4813)
                     /locus_tag="ACHXOL_003786"
     CDS             complement(4650..>4813)
                     /locus_tag="ACHXOL_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR6810650.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         1099 a         1265 c         1223 g         1226 t
ORIGIN      
        1 tagcgataat aaaaaaagcc tgccagatgg caggcttttt aataacggcg ttattactgc
       61 aacagcgaaa tatccgcaac gcgcaggaac agttcgcgca gtttctccag catggtcaga
      121 cggttgagac gcaattcttt gtcatcaacc atgaccatca ctttatcgaa gaaagcatca
      181 accggctcac gcagctctgc cagttcgacc agcgcatcct ggtaacgacc ttccgcaaag
      241 tacggctcca gcttgtcacg tagcaccaca acctgcatcg ccagtttaat ttcttccggc
      301 tctttcaggg tagaggcatt cacgcggtcg ctcagcactt cgtcagattt cgccagaatg
      361 ttagatacac gtttgttcgc cgccgccagt gcagcagctg catccagggt acggaaatgc
      421 gataccgctt tcatacgggc atcgaaatca gccggacgag tcggacgacg cgccagtacc
      481 gcctggatgg tgtcaacggt gtaaccttcg tcctgatacc aggcgcggaa gcgaccgagc
      541 ataaagtcga taacatcatc aactacgttg gcgttagtca gcttatcgcc atacagacgc
      601 actgcttctt cggtcagcgt ttgcagatca aggttgaggt tcttctcaac gataattcgc
      661 agcacgccaa gtgcggcacg acgcagcgca aacgggtctt tgtcgccttt cggatgctga
      721 ccgataccga agatacccgc cagagtatcc atcttatcag caatcgccag cgcacaagct
      781 accgggttgg acggcaggtc atcaccggca aagcgcggct gatactgctc attcagcgcg
      841 acggctacgt cttccgcttc gccatcgtga cgcgcgtagt gcatccccat aacgccttgg
      901 gtgtcggtga actcgaagac catgttggtc atcaggtcac acttagacag caggcccgca
      961 cgggttgcgt ggttaacgtc agcgccaatc tgttcagcaa tccagccagc cagcgcctgg
     1021 atgcggtcag ttttgtcacg cagcgtaccc agctgttgct ggaacagcac ggtttgcagg
     1081 cgcggcaggt tatcttccag acgtttttta cggtcggtat tgaagaagaa ctcggcatcc
     1141 gccagacgtg gacgaacgac tttctcgtta ccagagataa tttgctgcgg atctttcgat
     1201 tcgatgttgg caacgaagat gaagttcggc agcagtttgc catcgttcgc atacaccggg
     1261 aagtatttct ggtcaccttt catggtgtaa accagggctt cagccggcac cgcgaggaat
     1321 ttctcttcga attttgcggt cagaacgacc ggccactcca ccagcgaagc cacttcttcc
     1381 agcaggcttt cgcttaagtc agcgttaccg ccaatcttac gcgctgcttc ttcggcatcg
     1441 gctttaatct tcgctttacg ttcttcgtaa tcggcgatga ctttcccgcg ctcacgcaga
     1501 atttccggat actgatcggc gttgtcgatg gtgaattccg gttcgcccat aaagcggtgg
     1561 ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg ccggaatgac tttgtcgccc
     1621 agcagcaggg tcacggtgtg caccggacgc acgaagtgca cgtcgcttgc gccccaacgc
     1681 atcagtttcg ggatcggcag tttcgccagc gaagtcgcaa ccatattcgg cagtagtgct
     1741 tcagtgcttt cgcccttcac atgggcgcga tacagcagcc attcgccttt atcggtagtc
     1801 agacgctcag cctggtcaac ggtaataccg caaccacgcg cccaaccttc tgccgctttg
     1861 ctcggtttgc cttcagcgtc gaacgcctgg gcaatcgccg ggccgcgttt ttcgatttca
     1921 cgatctggtt gcgcttccgc caggttagct actttcaacg ccagacgacg cggagcagca
     1981 aaccattgaa cggtgccgtg tgcgaggcca gcgttatcca gctccgcagt aaagttcgca
     2041 gcaaaggact cagccaggct gcgcagtgct tttggtggca gctcttcagt gccgatttcc
     2101 accagaaaag ttttctcaga catagccgcc tcttacttat ctttgttgca catcgggaag
     2161 ccgagggctt cacgggaagc gtagtatgct tctgccactg ctttggtcag ggtgcgaatg
     2221 cgcagaatgt agcgctgacg ctcagtgacg gagatggctt tacgcgcatc cagcaggttg
     2281 aagctgtggg cggctttcag aatacgctcg taggctggca gcggcagcgg attttccagc
     2341 gccagcagct gctgcgcttc tttctcgtac tgctcgaagc aggtgaacag gaaatccaca
     2401 tccgcgtatt cgaagttgta agtggactgc tccacttcgt tctgatggaa cacgtcgccg
     2461 taggtggttt tacccagcgg gccgtcgctc cagaccaggt cgtaaacgct gtctacgccc
     2521 tgaatgtaca tggccagacg ttccagaccg taggtgatct cgccggtaac cggtttacac
     2581 tccagaccac caacctgctg gaagtaagtg aactgcgtca cttccatgcc gtttagccac
     2641 acttcccagc ccagtcccca ggcacccaga gtcgggtttt cccagttatc ttccacgaaa
     2701 cggatgtcgt gaatagtcgg gtccatgccc agctctttca gagaaccgag gtacagctcc
     2761 tgaatattgt ccggcgatgg cttaatgacc acctggaact gatagtagtg ctgtaaacgg
     2821 ttggggtttt cgccgtagcg accatcggtc gggcgacgag aaggctgaac ataagcagcc
     2881 gccatcggtt ctggccccag cgcgcgcaga caggtcattg ggtgagaggt tcccgcgccg
     2941 acttccatgt ccaatggttg aacaatggtg cagccctggc gagcccagta atcctgtaag
     3001 gtcaggatca agccctggaa ggtcctggta tcaaactttt gcatattatt tcgtgctgga
     3061 tacgtgtgga tttaaaggaa gggatcagta tacccgctgg atggaagata tacagtacga
     3121 aacgggaaaa agcagggctt aacgcatgga aagatgcaaa aaatggccat ctgcgtcaaa
     3181 agaacagaca aactgctctt tacggaaggt ataaccgcgc atttcatagc ttccctggaa
     3241 ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg ttataacgtt ttgccgcctg
     3301 atccttgcat aacgcttcca tattcaacga acgcagagga tcaaccttta cccgttgtgc
     3361 tttctgcacg ggtgacggtg tagaccaacc cactagcacc ataagcgcca ttgccggaaa
     3421 gaaagcgttc atcatcatta aattaccgtc tggtttgcag gtgagtctta ttatttatat
     3481 cggtatagtg gctgatatct tacgaatttc cttaagcctc tataagctgt agtgataaaa
     3541 ctcagattat tccagtgcgc caaccaaatg atttatcggt cagacttttt ttaaccagat
     3601 acagagacac agatgcagcc caaaatttac tggattgata acctgcgagg gatagcgtgt
     3661 ttaatggtgg tgatgattca caccactacc tggtatgtga ccaatgctca tagtgttagc
     3721 cccgtcacat gggatatcgc caatgttctg aattctgcct ctcgtgtcag cgtgccgcta
     3781 tttttcatga tttccggcta tctctttttt ggcgaacgca gcgcccagcc gcgccatttc
     3841 ttgcgtatcg gcttatgtct gattttttat agcgcaatcg cactgctcta cattgcgctg
     3901 tttacctcca tcaatatgga gttagcgctg aaaaacctgc tgcaaaagcc agtgttttac
     3961 cacttgtggt ttttcttcgc gattgcggtg atttatctgg tttcaccgct gattcaggtg
     4021 aagaacgtcg gcggaaaaat gttgctggtg ctaatggcgg tgattggcat tatcgctaac
     4081 ccaaacacag tgccgcagaa aattgacggt tttgaatggc tgccaattaa cttatatatc
     4141 aatggcgata ctttttacta cattctgtat ggcatgttgg gccgcgctat agggatgatg
     4201 gacacacagc ataaagcact gtcgtgggtg agcgccgcgc tgtttgcgac gggggttttt
     4261 attatctctc gcgggacatt atatgaattg cagtggcgcg gaaattttgc cgatacctgg
     4321 tatctttact gtgggccgat ggtttttatc tgcgcaatcg cgctattgac tctggttaaa
     4381 aacacgctgg atacgcgtac cattcgcgga cttggcttaa tctcccgcca ttcattgggt
     4441 atatacggat tccacgcctt gattatccat gcgctgcgca tccggggaat tgagcttaaa
     4501 aattggccaa tactggatat tatttggatc ttttgcgcga cgttggcagc gagtttgtta
     4561 ctttctatgc tggtacaacg aatcgacaga aacagattag tgagttaagt aaaagcccgt
     4621 tcacattgga ctgaccgggc ttacgtgaat tattcgctgt attcttcctg ggtaacctgt
     4681 gtttcttttg gcgggtttat tccggcatca cgaatattct tctgcaccgc tactgcacca
     4741 aacaagctta agaagaaagc cagtccataa aaaccttttt cactgagtaa taatgtcgca
     4801 ttccacagac cta
//
LOCUS       DAZRUF010000266         4810 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZRUF010000266 DAZRUF010000000
VERSION     DAZRUF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.268"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHXOL_003787"
     tRNA            6..81
                     /locus_tag="ACHXOL_003787"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHXOL_003788"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHXOL_003788"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHXOL_003789"
     CDS             complement(299..1057)
                     /locus_tag="ACHXOL_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR6810651.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHXOL_003790"
     CDS             complement(1065..2168)
                     /locus_tag="ACHXOL_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR6810652.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHXOL_003791"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHXOL_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4466)
                     /locus_tag="ACHXOL_003792"
                     /pseudo
     CDS             complement(3427..4466)
                     /locus_tag="ACHXOL_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
BASE COUNT         1319 a         1243 c         1148 g         1100 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcattc agcattcagc
     3721 atggcgaaaa gcgtccagcg tacaatcgag aaccattcat catcgccacg acgaactacc
     3781 ggcccaagcg gttctttaga gataacctcc ggtaagacaa tccattcagc tgggttgctt
     3841 aatttgatgc gcagggcata cagttgcgac tgatccgagg ccagcgtatc gcagcgacca
     3901 gattccagtg ccttcgctga ttcgtcagag cgatcgaaag tcaccggtgt gtacttcata
     3961 ttgtttgcct tgaaatagtc ggcgacgttg agttcggtat ctgtacccgc ctgaatacag
     4021 acggtagcgc catccagttc tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag
     4081 ccaatgccgt cgtaatcagt gacgccagta aatgccattc ccatcccggc atcgcgagat
     4141 gaagtccagg tcgtattacg ggagagcaaa tccacctccc ctgactgtaa agcggtgaag
     4201 cgttcttttg cagtgagcgg ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg
     4261 gcaacaccac gacaaacatc tacatcaata cctgaaaact taccgtctgc atcggcataa
     4321 gagaacccag gtaatccatc actgatcccg cattgcacaa aacctttttt ctgtacggca
     4381 tcaagcgtcg cgccagcatg cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc
     4441 agtgtggcta tcatcatctt tttcataatg cttcctgtga ggcgaaaatt atcgttatag
     4501 gacgtttggg aaacgtcttt tacctgtctg tggccatcct cttataagca aaagtagtgc
     4561 catcttttgc gcacgatttc gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa
     4621 cagcgccccg caatggtgcg aaaatgaaac atgcgccaca ttttggcgca aaatatttac
     4681 gcgtgtttta ctaagcagaa taatgattgt tagcgtgagt atatttctgg tgggaggtgt
     4741 gaaagttaaa ttgatgcgtt atttcgaaag cagcgaaata acgcgatata acatgctaag
     4801 gtaatgactc
//
LOCUS       DAZRUF010000267         4757 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZRUF010000267 DAZRUF010000000
VERSION     DAZRUF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.269"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..429
                     /gene="soxR"
                     /locus_tag="ACHXOL_003793"
     CDS             <1..429
                     /gene="soxR"
                     /locus_tag="ACHXOL_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR6810653.1"
                     /translation="TPGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRY
                     VAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRD
                     ELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            975..2324
                     /gene="ghxP"
                     /locus_tag="ACHXOL_003794"
                     /gene_synonym="yjcD"
     CDS             975..2324
                     /gene="ghxP"
                     /locus_tag="ACHXOL_003794"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR6810654.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2476..4125
                     /locus_tag="ACHXOL_003795"
     CDS             2476..4125
                     /locus_tag="ACHXOL_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR6810655.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4279..>4757)
                     /locus_tag="ACHXOL_003796"
     CDS             complement(4279..>4757)
                     /locus_tag="ACHXOL_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR6810656.1"
                     /translation="DLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTN
                     TFLEMVICTDSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGI
                     SILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT          984 a         1136 c         1326 g         1311 t
ORIGIN      
        1 acccccggcg aagtggcgaa acgcagcggt gtggcggtat cggcgctgca tttctatgaa
       61 agtaaagggt tgattaccag tatccgtaac agcggcaatc agcggcgata taaacgtgat
      121 gtgttgcgat atgttgcaat tatcaaaatt gctcagcgta ttggcattcc gctggcgacc
      181 attggtgaag cgtttggcgt gttgcccgaa gggcatacgt taagtgcgaa agagtggaaa
      241 cagctttcgt cccaatggcg agaagagttg gatcggcgca ttcatacctt agtggcgctg
      301 cgtgacgaac tggacggatg tattggttgt ggctgccttt cgcgcagtga ttgcccgttg
      361 cgtaacccgg gcgaccgctt aggagaagaa ggtaccggcg cacgcttgct ggaagatgaa
      421 caaaactaaa gcgccacaag ggcgctttag tttgttttcc ggtctttgtc tttctctcta
      481 tcccgctggt acacaggagg gtttcccccg acgtcaacac accccattcg agcacgtggt
      541 ggaggttccg gttggtgttg atgctttaat tgtatgtcac cggcatttct tcgccagtgt
      601 aaaagtatac tttttatccg caatattttt gtcatctcag acgatttttt atcgcaatcc
      661 tgaacggtat acggctcgat aacgctgcaa tcttgcgcac cgacgataac gtttgcgcat
      721 caattgcctg gtttttcatc gtcaagacaa taaaagagaa aaaagcagca aacttcggtt
      781 gaaaaagccg ctatgatcgc cggataatcg tttgcttttt ttaccacccg ttttgtatgc
      841 gcggagctaa acgtttgctt ttttgcgacg cagtaaattg tcgcaaacct ggagcaggaa
      901 gataacgttt cgctggcagg ggattgtccg ccacgcatct tgacgaaaat taaactctca
      961 ggggatgttt tcttatgtct acgccatcag cgcgtaccgg cggttcactc gacgcctggt
     1021 ttaaaatttc acaacgtgga agcactgtcc gtcaggaagt ggttgccggg ttaacaacgt
     1081 ttctggcgat ggtctactcg gtcatcgtcg ttccaggtat gttgggtaaa gcaggcttcc
     1141 cgcctgcggc agttttcgtt gcaacctgtc tggttgccgg actcggttct atcgtgatgg
     1201 gtctgtgggc taatctgccg ttggcgattg gttgcgccat ctccctgacg gcgtttaccg
     1261 cattcagcct ggtgctgggg caacatatta gcgtacctgt cgcgctgggt gccgtgttcc
     1321 tgatgggtgt gctgtttacg gtaatttctg ccacgggtat ccgtagctgg attttgcgca
     1381 acttgccgca cggtgtggcg cacggcacgg ggatcggtat cggtctgttc ctgctgctca
     1441 ttgccgctaa tggtgtcggt ctggtgatta aaaacccgct tgatggtctg cccgttgcgc
     1501 tgggtgattt cgcgaccttc ccggtgatta tgtcactggt aggtctggcg gtgatcatcg
     1561 gcctggaaaa actgaaagtc cctggtggca ttctgctgac cattatcggt atctcaattg
     1621 tcggtttgat cttcgatcct aacgtccatt tctccggcgt tttcgccatg ccttcattga
     1681 gcgatgaaaa cggcaattca ctgattggca gcctggacat tatgggcgcg ctgaatcctg
     1741 tagtcctgcc aagcgttctg gcgctggtga tgacggcagt atttgatgcc accggaacta
     1801 tccgtgccgt cgccggccag gcgaacctgc tggataaaga tgggcagatc atcgacggtg
     1861 ggaaagcact gaccactgac tccatgagca gcgttttctc tggcctggtg ggtgctgctc
     1921 cggcagcggt atacatcgag tctgcggcgg gtacggcggc gggcggtaaa accggtttga
     1981 cggctatcac cgttggcgtg ctgttcctgc tgattctgtt cctctctccg ctctcttacc
     2041 tcgttccggg gtatgcaacg gctccggcgc tgatgtacgt tggcctgctg atgctgagca
     2101 acgtggcgaa aatcgacttt gctgattttg ttgatgcgat ggcgggtctg gttacggcgg
     2161 tattcatcgt gctgacctgt aacatcgtaa caggcatcat gatcggcttc gcgactctgg
     2221 tgattggtcg tctggtttcc ggcgaatggc gcaagttgaa catcggtacg gtcgttatcg
     2281 ccgtggcgct ggtgaccttc tatgcgggtg gctgggctat ctaatctttc atcggattct
     2341 gaaaacgggt ggcaatggct gcccgttttt attttctccg cacattgtgt gagcttttgc
     2401 gatattctga aaaaatgaga attcaggcat aacgcctttc tcagatcata aaaaaacatc
     2461 gcaaacaggg aacgcatgga aatcttcttc accatactga taatgaccct cgtggtctca
     2521 ctgtccgggg tggtcactcg tgtcatgccc tttcagatcc cgcttccgct tatgcaaatc
     2581 gccatcggtg cgctactggc gtggccgacg tttggtttgc atgtggagtt tgatcctgaa
     2641 ctctttttag tcttgtttat cccgccgttg ctgttcgctg atggctggaa aacgccgacc
     2701 cgtgaatttc tcgaacatgg tcgagagatt ttcggcctcg cgctggcgct ggtggtggtc
     2761 accgtggtcg gcattggctt ccttatttac tgggtggtgc cgggcattcc gctgatcccc
     2821 gcctttgcgc tggcggcggt actttctccg accgatgctg tggcgctctc cgggattgtt
     2881 ggcgaagggc gcatcccgaa aaaaatcatg ggcattttgc agggcgaagc gttgatgaat
     2941 gacgcctccg gcctggtgtc gttgaagttt gccgtggcag tggcgatggg gacgatgatc
     3001 ttcaccgtcg gcggtgcgac ggtcgaattt atgaaagtag ccattggtgg tattcttgcc
     3061 ggttttgtgg tgagctggct gtatggtcgc tcgctgcgat tcctcagccg ctggggcggt
     3121 gatgaacccg cgacgcagat cgttctgctg ttcttgctgc cattcgcttc ttatctgatt
     3181 gccgaacata ttggcgtttc cggcatcctc gctgcggttg ccgccgggat gaccatcacc
     3241 cgctccggtg tgatgcgccg tgcgccgctg gcaatgcgcc tgcgcgcaaa cagcacctgg
     3301 gcgatgctgg aatttgtctt taacggcatg gtgttcctgc tgttaggtct gcagctgccg
     3361 ggtattctgg agacgtcgct gatggcggca gaaatcgacc ctaacgtcga aatctggatg
     3421 ctgtttaccg atattattct gatatatgcg gcgctgatgc tggtccgttt cggctggctg
     3481 tggacgatga aaaagttcag caaccgcttc ctgaagaaga agccgatgga gtttggttcg
     3541 tggaccacac gagaaatcct gatcgcgtct ttcgccgggg tgcgtggggc gatcactctg
     3601 gccggtgtgc tctctatccc gctgctcttg ccggatggta acgtcttccc ggcgcgctat
     3661 gagctggtgt tcctggcggc tggcgtcatt ctcttctcgc tgtttgtcgg cgtggtgatg
     3721 ttgcctattc tgctacaaca cattgaagtc gcggaccatt cgcaacaatt gaaagaagaa
     3781 cgtattgcgc gagcggcaac ggcagaagtg gcgattgtgg cgatccagaa aatggaggag
     3841 cgtctggcgg cggataccga agagaatatc gataaccagc tgcttacgga ggtcagttct
     3901 cgcgtcattg gtaacctgcg tcgtcgcgcc gatggacgta acgacgttga aagttccgtg
     3961 caggaagaga accttgagcg tcgcttccgt ctggcggcat tgcgttctga acgtgctgaa
     4021 ctgtaccacc tgcgcgccac gcgggagatc agcaacgaaa cgctgcaaaa attactgcac
     4081 gatctcgatt tgcttgaagc gttgctaatt gaggaaaatc agtaagccga aagcgacgta
     4141 accaggcccg aggtaatctg aaaaaggtgc aatcttttgc acctttaaca ggttagctcg
     4201 ggctaacatt catgattcta aaacaaataa gttatctctc ttgtaaagtc aattcattgg
     4261 ctgttcttcg acttaacctc aatctgcgtg ttgcataata tctgcagtaa tcttcatagc
     4321 gactgcatta atattatcct cagtttgtga catgccagta tgagaggtac aatcttcttc
     4381 aaagaatttc tgtagtgtta ccagggtatc actatcaatg gggatatttt cctccccacg
     4441 gagtatggaa atgcccattc cctggacgct agcctcatca aaattaaccc gatagagacg
     4501 ggtattaaac atacaggctt ttgttaaaat agaacagttg aaatggcagt tgcttaaatt
     4561 ggcattatta aaattaacat tagccatatt agagtcggta catataacca tttcgaggaa
     4621 tgtatttgtt agccacgtac aaaagagttt gctatcaatt aagtttatat tctggaggat
     4681 tgctttgttc agattgacgc gagacagatc agcatgttca aagttaatta aatcgagttt
     4741 agagaaacta aggtcgg
//
LOCUS       DAZRUF010000268         4714 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZRUF010000268 DAZRUF010000000
VERSION     DAZRUF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.270"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..737)
                     /locus_tag="ACHXOL_003797"
     CDS             complement(<1..737)
                     /locus_tag="ACHXOL_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR6810657.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHXOL_003798"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHXOL_003798"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR6810658.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="ACHXOL_003799"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="ACHXOL_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR6810659.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="ACHXOL_003800"
     CDS             4397..4678
                     /locus_tag="ACHXOL_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR6810660.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1134 a         1173 c         1185 g         1222 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg attttaattg catttagaaa gccg
//
LOCUS       DAZRUF010000269         4711 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZRUF010000269 DAZRUF010000000
VERSION     DAZRUF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.271"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            64..1026
                     /gene="tauA"
                     /locus_tag="ACHXOL_003801"
     CDS             64..1026
                     /gene="tauA"
                     /locus_tag="ACHXOL_003801"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR6810661.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1039..1806
                     /gene="tauB"
                     /locus_tag="ACHXOL_003802"
     CDS             1039..1806
                     /gene="tauB"
                     /locus_tag="ACHXOL_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR6810662.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1803..2630
                     /gene="tauC"
                     /locus_tag="ACHXOL_003803"
     CDS             1803..2630
                     /gene="tauC"
                     /locus_tag="ACHXOL_003803"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR6810663.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2627..3478
                     /gene="tauD"
                     /locus_tag="ACHXOL_003804"
     CDS             2627..3478
                     /gene="tauD"
                     /locus_tag="ACHXOL_003804"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR6810664.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3584..4558)
                     /gene="hemB"
                     /locus_tag="ACHXOL_003805"
     CDS             complement(3584..4558)
                     /gene="hemB"
                     /locus_tag="ACHXOL_003805"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR6810665.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1048 a         1218 c         1380 g         1065 t
ORIGIN      
        1 cttttaatga atgtttttat tcctgaatac tgctcccata acaagacagg ggagcagaca
       61 atcatggcaa tttcatcgcg taacacactt cttgccgcac tggcattcat cgcttttcag
      121 gcacaggcgg tgaacgtcac cgtggcgtat caaacctcag ccgaaccggc gaaagtggct
      181 caggccgaca acacctttgc taaagagagc ggggcgagcg tggactggcg taagtttgac
      241 agcggagcca gcatcgtgcg ggcgctggct tctggcgacg tgcaaatcgg caacctcggt
      301 tccagcccat tagcggttgc agccagccaa caggtgccga ttgaagtctt cttgctggcg
      361 tcaaaactgg gtaactccga agcgctggtg gtaaagaaaa ctatcagcaa acccgaagat
      421 ttgattggca aacgcattgc cgtaccgttt atctccacca cccactacag cctgctggcg
      481 gcgctgaaac actgggggat taaaccaggg caagtggaga ttgtgaacct gcagccgccc
      541 gcgattatcg ccgcatggca gcggggtgat attgatggtg cttatgtctg ggcaccggcg
      601 gttaacgccc tggaaaaaga cggcaaggtg ttgaccgatt ctgaacaggt cgggcagtgg
      661 ggtgcgccaa cgctggacgt ctgggtggtg cgcaaagatt ttgccgagaa acatcctgag
      721 gtcgtgaaag cgttcgctaa aagcgccatc gatgctcagc aaccgtacat tgctaaccca
      781 gacgcgtggc tgaaacagcc ggaaaacatc agcaaactgg cgcgtttaag cggcgtgccg
      841 gaaggtgacg ttccggggct ggtgaagggg aatacctatc tgacgccgca gcaacaaacg
      901 gcagaactga ccggaccggt gaacaaagca atcatcgaca ccgcgcagtt tttgaaagag
      961 cagggcaagg tcccggctgt agcgaatgat tacagccagt acgttacctc gcgcttcgtg
     1021 caataaaagg aggcgcagat gctgcaaatc tctcatctct atgccgatta tggcggcaaa
     1081 ccggcactgg aagatatcaa cctgacgctg gaaagcggcg agctactggt ggtgctgggg
     1141 ccgtccggct gcggtaaaac caccctgctg aatctgattg ccggttttgt gccttatcag
     1201 catggcagca ttcaactggc gggtaagcgt attgagggac cgggagcaga gcgcggggtg
     1261 gtttttcaga atgaagggct actaccgtgg cgcaatgtac aggacaacgt ggcgttcggc
     1321 ctgcaattgg caggtataga gaaaatgcag cgactggaaa tcgcgcacca gatgctgaaa
     1381 aaagtggggc tggaaggcgc agaaaaacgc tatatctggc agctttccgg tggtcaacgt
     1441 cagcgggtgg ggattgcccg cgcgctggcg gcgaatcccc agctgttatt actcgacgaa
     1501 ccgtttggtg cgctggacgc cttcacccgc gaccagatgc aaactctgct gctgaaactc
     1561 tggcaggaga cgggcaagca ggtgctgttg attacccacg atatagaaga agcggtgttt
     1621 atggcgactg aactggttct gctttcatcc ggccctggcc gtgtgctgga gcggctgccg
     1681 ctcaactttg ctcgccgctt tgttgcggga gagtcgagcc gcagcatcaa gtccgatcca
     1741 caattcatcg ccatgcgcga atatgtttta agccgcgtat ttgagcaacg ggaggcgttc
     1801 tcatgagtgt gctcattaat gaaaaactgc attcgcggcg gctgaaatgg cgctggccgc
     1861 tctcgcgtca ggtgacctta agcattggca cgttagcggt tttactcact gtatggtgga
     1921 cggtggcggc gctgcaactg attagcccgc tgtttttgcc gccgccgcaa caagtactgg
     1981 caaaactgct caccattgcc ggaccgcagg gatttatgga cgccacgctg tggcagcatc
     2041 tggcagccag cctgacgcgc attgtgctgg cgctactcgc ggcggtggtg atcggtattc
     2101 cggtcgggat cgccatgggg ctaagcccga cggtgcgcgg cattctggat ccggtaatcg
     2161 aactttatcg tccggtgccg ccgctggctt atttgccgct gatggtgatc tggtttggta
     2221 ttggtgaaac ctcgaagatc ttattgatct atttagcgat ttttgcaccg gtggcgatgt
     2281 cggtgctggc gggggtgaaa agcgcgcagc aggttcgcat tcgtgccgcc cagtcgctgg
     2341 gtgccagccg tgcgcaggtg ctgtggtttg tcattttgcc cggcgcgctg ccggaaatcc
     2401 tcaccggatt acgcattggt ctgggtgtgg gctggtctac gctggtagcg gcggagctga
     2461 ttgccgcgac acgcggttta ggatttatgg ttcagtcagc gggtgaattt ctcgcgactg
     2521 acgtggtact ggcggggatc gcggtgattg cgattatcgc ctttctttta gaactgggtc
     2581 tgcgcgcgtt acagcgccgt ctgacgccct ggcatggaga agtacaatga gtgaacgtct
     2641 gagtattacc ccgctggggc cgtatatcgg cgcacaaatt tcgggtgccg acctgacgcg
     2701 cccgttaagc gataatcagt ttgaacagct ttaccatgcg gtgctgcgct atcaggtggt
     2761 atttctgcgc gatcaagcta ttacgccgca gcagcaacgc gcgctggccc agcgttttgg
     2821 cgaactgcat attcaccctg tttaccctca tgccgaaggg gttgacgaga tcatcgtgct
     2881 ggatacccat aacgataatc cgccggataa cgacaactgg cataccgatg tgacatttat
     2941 tgaaacgcca cccgcagggg cgattctggc agctaaagag ttaccttcga ccggcggtga
     3001 tacgctctgg accagcggta ttgcggccta tgaggcgctc tctgttccct tccgccagct
     3061 gctgagcggg ctgcgtgcgg agcatgattt ccgtaaatcg ttcccggaat acaaataccg
     3121 caaaaccgag gaggaacatc aacgctggcg cgaggcggtc gcgaaaaatc ctccgttgct
     3181 acatccggtg gtgcgaacgc atccggtgag cggtaaacag gcgctgtttg tgaatgaagg
     3241 ctttactacg cgaattgtcg atgtcagcga gaaagagagc gaagccttgt taggcttttt
     3301 attcgcccat atcactaaac cggagtttca ggtgcgttgg cgctggcaac cgaatgatat
     3361 tgcgatttgg gataaccgcg tgactcagca ctatgccaat gccgattacc tgccacagcg
     3421 gcggattatg catcgggcga cgatccttgg ggataaaccg ttttatcggg cggggtgata
     3481 cgagtgcgga cggttccctc gcccccttgg ggagagggtt agggtgaggg ggcgttcacc
     3541 gtactttcaa caggttaact ccccctttct gagaggaaaa aaattaacgc agaatcttct
     3601 tctcagccaa atccagcgca aagtagctga aaatcagatc cgcacccgca cgcttaattg
     3661 aacctaagct ttcgagcacg actttctctt catctatagc acccgccagc gcggcgaact
     3721 taatcatcgc gtactcaccg ctcacctgat acgcgccaat cggcaattca gtacgttcac
     3781 gcagctcacg cacgatgtcg aggtacgctc cagcaggttt aaccatcagg cagtctgcgc
     3841 cctgggcttc atccagcagt gattcacgaa tcgcctcgcg acggttcatt gggttcatct
     3901 gatagctttt gcggtcgcct tttaatgcgc ttccggcagc ttcacggaac gggccataaa
     3961 aggaagaggc gaacttggtc gaatacgaca taatcgccgt atctttaaag cctgcagcgt
     4021 ccagcgcctg acgaatcgcc tgtacctggc cgtccatcgc ggcggaaggg gcgatgaagt
     4081 ctgcgcctgc agcagctgca accacggctt gcttgcctaa attttccaga gtcgcgtcgt
     4141 tgtcgacgcc atgctcgcac agcacaccgc agtgaccgtg agaagtgtat tcacagaaac
     4201 aagtgtctga catgacgatc atttctggca cagtctgctt gcagatgcgc gacatacgcg
     4261 ccaccagtcc atcttcccgc caggcgtcgc tgccggtttc atcggtatgg tgagagatgc
     4321 caaaagtcat tacggaacgg ataccggcgt tggcgatgcg ttcaatttcg cgtgccagat
     4381 gtttctctgg aatgcgcatc acgcctggca tggcttcaac ggctttgtag tcgtcaattt
     4441 cttcttcaac aaagatcggc aacaccaggt cgttaaggct aagtgttgtc tcttcaaaca
     4501 tagcgcgcag cgcaggagat ttgcgtaggc gacgagggcg ttggattaag tctgtcatgg
     4561 tctgcctgat gtttgtggaa ttaaggtgca cagtatacct gaagcggggc aggagtgttt
     4621 tacgaaagtt gtcgcgatgt tgacaagaag agaatgaaag agagaccagg acatctttgt
     4681 aatagaaata gcaataattt catattcact g
//
LOCUS       DAZRUF010000270         4656 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZRUF010000270 DAZRUF010000000
VERSION     DAZRUF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.272"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHXOL_003806"
     CDS             complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHXOL_003806"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR6810666.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHXOL_003807"
     CDS             complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHXOL_003807"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR6810667.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1006 a         1325 c         1210 g         1115 t
ORIGIN      
        1 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttactta
       61 atgggcaaaa aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg
      121 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
      181 aattactttt tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag
      241 ggtggtgtca taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
      301 aatcgcgcga cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
      361 gtcctgaatg tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc
      421 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
      481 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg
      541 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
      601 ggtgcggccc acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc
      661 aacacccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
      721 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
      781 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
      841 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc
      901 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
      961 ctgacttaag gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg
     1021 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     1081 gtcaacttct accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac
     1141 gctgaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     1201 ccgaccgccg agaacgtaag acggacgtac caccagtggg tagccaatct ctttcgcctt
     1261 ctctaccgcc atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     1321 ctcaaccgca tgctggaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt
     1381 gccgataacc ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc
     1441 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcca gcacatcttc
     1501 cagagttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc
     1561 cgggttacag ttaaccataa tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg
     1621 tacacagcag tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac
     1681 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     1741 catgtaagcg gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg
     1801 gtgcaggtca tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt
     1861 tgccagacgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     1921 gccagtgatg cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac
     1981 caggaaccag cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg
     2041 gaaggcatcg gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat
     2101 tttggttaac gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac
     2161 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
     2221 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa
     2281 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
     2341 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
     2401 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac
     2461 gcgtgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
     2521 attggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
     2581 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt
     2641 atggatgccc atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt
     2701 atcacgcacc acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcag
     2761 ctctttggtt ggggagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
     2821 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
     2881 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
     2941 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
     3001 aatcgcatcg gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg
     3061 ctccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc
     3121 cgggcgctct ttttcaataa tcttgcgtac tacttcccag tgaatcggct cgatgtaggt
     3181 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
     3241 gcggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
     3301 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
     3361 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
     3421 tcgataaagt ggtcgaacaa cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga
     3481 ccctggaagc tgaacgccgg tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac
     3541 agggatttat gcgtgacacg caggtttgca ggtaatgttg cttcatccac cgcaaaaccg
     3601 tggttctggg cggtgatcat caccacgttt ttctccacat ctttaaccgg atggttgccg
     3661 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
     3721 tgaccgaggc agatgccgaa taccggaata tcggtttcga ggaatttctg gatggcggta
     3781 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga
     3841 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
     3901 ctatccacca gcatccgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
     3961 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
     4021 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga
     4081 cctgggaacg cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata
     4141 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata
     4201 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg
     4261 aagttgctgg caatcagcgg cagatcgcga atcaccagac cttgtgcatg tacctgagaa
     4321 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
     4381 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
     4441 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
     4501 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
     4561 actttatttg catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac
     4621 gaatttctgg caaacggcgg cattctggag atatgg
//
LOCUS       DAZRUF010000271         4609 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZRUF010000271 DAZRUF010000000
VERSION     DAZRUF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.273"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            48..872
                     /gene="rcnA"
                     /locus_tag="ACHXOL_003808"
     CDS             48..872
                     /gene="rcnA"
                     /locus_tag="ACHXOL_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR6810668.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1091..1429
                     /gene="rcnB"
                     /locus_tag="ACHXOL_003809"
     CDS             1091..1429
                     /gene="rcnB"
                     /locus_tag="ACHXOL_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR6810669.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1511..2530)
                     /locus_tag="ACHXOL_003810"
     CDS             complement(1511..2530)
                     /locus_tag="ACHXOL_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR6810670.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNANLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATIN
                     SKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFMLESNGS
                     PVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALI
                     NVKYD"
     gene            complement(2546..>4609)
                     /locus_tag="ACHXOL_003811"
     CDS             complement(2546..>4609)
                     /locus_tag="ACHXOL_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR6810671.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
BASE COUNT         1233 a         1126 c          947 g         1303 t
ORIGIN      
        1 ggggggagta gaatcagatt gccgaattaa tactaagaat tattatcatg accgaattta
       61 caactcttct tcagcaagga aacgcctggt tcttcatccc cagcgccatc ttacttggtg
      121 cgcttcatgg cctagaacca gggcactcaa aaacgatgat ggcggcgttt atcatcgcca
      181 tcaaaggcac cattaaacaa gcggtgatgc tcggactggc agcaactatt tcgcataccg
      241 cagtggtctg gttaattgcc tttggcggga tggtgatcag caagcgcttt actgctcaat
      301 cagcagaacc gtggctccag ctgatttccg cagtgatcat tattggcacc gcgttctgga
      361 tgttctggcg tacctggcgc ggcgaacgca actggctgga gaatatgcac gagtatgatt
      421 atgagcatca tcatcacgat cacgaagatc accacgacca tggacatcat caccatcacg
      481 aacatggcga gtatcaggat gcccatgcac gagcccatgc caatgacatt aaacgacgct
      541 ttgatggtag agaggtcacc aactggcaaa ttttgctatt tggcttaacc ggtggcctta
      601 tcccctgccc ggcagcaatt accgtgctgt tgatatgcat tcagttgaaa gccctgacac
      661 tgggcgcaac actggtcgtc agtttcagca ttggcctggc gttaacgctt gtcaccgtag
      721 gcgttggcgc agcaatcagc gttcagcagg tcgcaaaacg ctggagcgga tttaacactc
      781 tcgctaaacg cgccccctat ttttccagtc tgttgattgg cttagtcggt gtgtatatgg
      841 gcgtacatgg cttcatgggc ataatgcgat aactcaatcc tttaggcttg catgttatct
      901 tcagcccagg atgattctta aatcagctat ttccgctgac tctctgctcg ctaaggatgc
      961 ttttagggca tcctttcatt tacacttttt acgaaatcat gggatcacta acaaaatatc
     1021 gcttgtcagt tatattgtat ggcaggaaag atatgcgact gatattacag atccccaaag
     1081 tggagagttt atgaccatta aaaataagat gttgctgggt gtgcttttgc tggttaccag
     1141 tgccgcctgg gccgcaccag ccaccgcggg ttcgaccaat acctcgggaa tttctaagta
     1201 tgagttaagt agtttcattg ctgactttaa gcatttcaaa ccaggtgata ccgtcccaga
     1261 gatgtaccgc accgatgagt acaacattaa gcagtggcag ttacgtaacc tgcccgcgcc
     1321 tgatgccggg acgcactgga cctatatggg tggcgcgtac gtgttgatca gcgacaccga
     1381 cggtaaaatc attaaagcct acgacggtga gattttttat catcgctaaa aaaagccccc
     1441 tcatcatgag ggggaaatgc agacaccttg ttatttttta ttattagcca cttgctcgtc
     1501 ttgcttgtta ttagtcgtat ttcacgttga ttaatgcggt tgcctccagt gcgccagatt
     1561 taactttgtt tgtatcgtag acgtagtaac tggcagttat cggaattgaa accgttgtat
     1621 ctgtaagttt tgactttgcc gcaatttctt ttaaatttgt gtatcctttg ctgttatcag
     1681 tgatatttaa aaatgtgact ggagaaccat tgctttcaag cataaatccg actccattat
     1741 tttctgcacc tttcaggatc gtgttactgc cggctaacaa atcagaactt gataaataca
     1801 cctgcatttt ccgtgatgtt tgattttgtt gacctaataa atttttacaa cgcagaataa
     1861 tatccccttg tttactattt attgttgctt tgttacctgt ggctggtaat tgtgaaacgg
     1921 gaatactgtc taaagtaatt aataaatcgt ccggtttgca ggttgtggtt atcgggttgt
     1981 atgacaatgt cagattagca ttttgtgcca atgcatttct gtagctattt acacatgcat
     2041 cccatgactt gccacctaat aaacacagta gaaattgtac tgcggcactc ccgcgtgtcg
     2101 atgatgaagc actggataat gtcgcaacac cattaatcat cactgaaccg ccgccccccg
     2161 cagaaacatt cacttttgca ttactggccg caggtgtcac cgtaactgaa taaccactgg
     2221 tgaaactggt ctgattattt gacaacgtga tgttgttttt attggtccat tcaaaatgta
     2281 aatttaattt tatggtatcg ttataaagcc caacaataat atccgacgaa ctcatagggg
     2341 tgatagtatt tgtactcacg catgacgaat cattaaaatt atgctcatat gtcgctattt
     2401 gtgtttgagt aagtgcaaaa tccccctgaa caaccaaatt gtgctgaaca gataactcac
     2461 tataacatgc cgcatagctc acaggcataa ccatcatcat taatataaat agcattatgc
     2521 gaatttccat agaaccacct gactattact ggcaaatata atttctactt tcatcaatct
     2581 ctttaccgaa ggtgattgtg catgaaagtc cttgttgctt atcaattgct acattaaccg
     2641 atggcggtat ttcattggtg cgaataaata actggcttcc ctggccgaca acgccaatat
     2701 tatgaccgtg gatatcattg acttcataac caaacgttaa tggttgccca tccgctctta
     2761 acgcttttat aaaccagggc ttgcgctgat cggtatcaaa attaaccagt acaaccgcgc
     2821 cgcgataagg tgcggcaatt ttccggttgc cacgtaattc tgcttcgcta tcgctttgcg
     2881 acacatccag catcaggtga ttttcccgat aaggtgtcat tccgtcgtat accaccactc
     2941 cattacggtt tgttgtgcga tatttttgcc cattgacata agcatcttta attcctggcg
     3001 cattcatcac agcaaacgtt tcggaaagac ggttcgccag attaacgcca cccgaccagg
     3061 cgacaatgcc ccctgaaaca ctggctccag cctgtcgata agtactcgac tgactataac
     3121 tgccattcac tgttgccacc ggcgcgttcc aggttaaatt cgctccagct gtcgtttcat
     3181 ttccctgatg ttgatgactc aggttgacac cataattgaa ctgatcccga ctccctactg
     3241 ttcctgataa tcccgtatta tttgaggcaa acccctgatc atcaaacgtc gttgagttag
     3301 acatatatat ttgccgacga ggcgtcgaaa cgtcatcacc ccaatcaaag ggaatcgata
     3361 taaaaatatt aaaacgtttc tcttcatgat gattctcgtc ataagcctgg cttgccgcga
     3421 gggtatagct tatccgtcgc aggttgttgg aataactcaa ctgataatcc ttactactgc
     3481 cgctacgccc ccagtaatct cgccataacg tacttaatga cacagacccc caaccttctg
     3541 gcaatgactg gctcatattg gcggaaaagc tatttttgcg accaaaatca ttctggtaat
     3601 aatcggcaat gtcatagaca tcgttttcat cacggcgata attatcttta ttgtttgccc
     3661 aaacgtgatc gttaaatgtc cggtaatcac gcgacgaata acgccaggcc gccagaccaa
     3721 aacgcgtcga cgtttggctc acaaatttgt tgtaggcaat ttgataactt tgcccgtcaa
     3781 acacatcgcc gttgtcttgt ttactatgcg acttcgtggc atcgacggaa atggcaccaa
     3841 tgcgtgtatt ccagccagtc cccaaagtga acgcgtaata attattcgca accatcgagc
     3901 caccatacaa cgtcaataag ttattaaaac catactgata acccgcctgg acaaaatcac
     3961 tatgtttgct cgccccttca atatggctac gacccgccgc aaaatcatat ttcgacacgc
     4021 cgggttgcag catatttggc accgctgcat aaggcaccag ataggtggtt accgaaccgt
     4081 ccgcctcttt cacgctgaca tcaagatctg ctccaccacc ggccaactgc aaatctgtaa
     4141 tcgcaaacgg gccaggagga acctctttct gataaaccac aaaaccattc tgttcaatag
     4201 ttaccagcgc gttactctga gcaatcccct gcacccgtgg cgtaaaattt tgtttcgagt
     4261 taggcaacat ctgcatatca cgaaacaacc gcacacctct gaagcgaaca gaatcaaaaa
     4321 tatcgcttga tgtgtacata tcacccacgc gaagcgtgcc gagaagttgg gcaaatccac
     4381 gttccagata cagggtattg cttttccaca cccctggatt gttatttgtt ttactgaaac
     4441 tggcatcaga atgcagttgc caccccagta aatttaaccc gctgttaaaa cgtacatatg
     4501 tactcttact attacccgac gctttatagt cactgtaata ctgactcaca taataagagg
     4561 tataaaacgc attaataccc cgctcccagt tttccggtgg aacatagcc
//
LOCUS       DAZRUF010000272         4489 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZRUF010000272 DAZRUF010000000
VERSION     DAZRUF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.274"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(9..248)
                     /locus_tag="ACHXOL_003812"
     CDS             complement(9..248)
                     /locus_tag="ACHXOL_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR6810672.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(251..970)
                     /gene="sanA"
                     /locus_tag="ACHXOL_003813"
     CDS             complement(251..970)
                     /gene="sanA"
                     /locus_tag="ACHXOL_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR6810673.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1120..2004)
                     /gene="cdd"
                     /locus_tag="ACHXOL_003814"
     CDS             complement(1120..2004)
                     /gene="cdd"
                     /locus_tag="ACHXOL_003814"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR6810674.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2134..2829)
                     /locus_tag="ACHXOL_003815"
     CDS             complement(2134..2829)
                     /locus_tag="ACHXOL_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR6810675.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2826..3224)
                     /locus_tag="ACHXOL_003816"
     CDS             complement(2826..3224)
                     /locus_tag="ACHXOL_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR6810676.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3463..4410
                     /gene="dusC"
                     /locus_tag="ACHXOL_003817"
     CDS             3463..4410
                     /gene="dusC"
                     /locus_tag="ACHXOL_003817"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR6810677.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKA
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         1198 a         1140 c         1153 g          998 t
ORIGIN      
        1 agaaaatatt aacgatagaa aataagataa acaatcatgc cgatagataa aattccgaag
       61 ccgatatatg ccaccatata agcgaaaaag agtccgggat tttttgctcg ccagacatac
      121 accggttccg gtgcattttt aacccgttta tcaatcgcgc ctgcgcgcca gcctttccat
      181 gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg caacgacaaa aaactgctgt
      241 acatccataa ctactttcct tgtttctttt gtaattcaag taactgttcg ggtgtgacgg
      301 cgggataccc ctgcgcatct tccggcacct ggtgcatagc cggaataggg accagcggcc
      361 ctaaaaaacg cggttcacgt ctaaaaatat aaaggtcagc cagcgcaccg aaacgggcgg
      421 caaattcgcg aatacgtact gacagcatat ctttcggtga cggtacggca taacactgag
      481 cctgaatccc catatgcagc gcaataaata atgcccgctc acagtggaaa cgttgggtga
      541 taataatgaa atcattagta tcgaacactt tgcgtgtacg cacgatggaa tccagcgtac
      601 gaaagcctgc gtaatcgaga acaatatctg atgggtcgac accagcagcg attaaatctt
      661 tgcgcatggt catcggctca ttataacttt gcaatgcgtt atcgccgctc agtaatagat
      721 aatttacctt accgctgtta taggcattaa tcgctccttg aatgcggtag cgataatact
      781 gattaattac gccagtacga taatattttg ctgtgccgag caccacgccg acctggcggt
      841 aggggagatc ctgcaattcg tcgtagatat aaggcgcggt tttccagctc atccagcgat
      901 cgaggccgag cacagtcaac agcagcaagc cgatcaggac taacaggctg aggaacacgc
      961 gctttaacat gcaggagact cgatctaagg aaaatttcca tcaggctact ttacccgctg
     1021 gttttgcgca agaaacggca aactgtttgc cgggaatttc gacaccagga gagggctggc
     1081 aagccggaca aggcgttcac gccgcatccg gcaccaggct taagcgagaa gcacgcggtc
     1141 gatactgtga cagccgagag ctttcagcgt tgcggaggtg gcatcccact gaatcaacgg
     1201 cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc gggtaatcat aacccttgag
     1261 attcaacaga attaacgccc cctgcaacgg tggcagagtc gggttgaatg cggcgttttc
     1321 agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc agcgcgacac cgcttggcga
     1381 cttactgtaa ggcatgtgcg aacggtttgc cgccgcaatc gctgcctgag aaagcgcatc
     1441 acccgtcagc gcatagccgt gatcctgttc gtccatcagc agcgttttaa tctccagatc
     1501 tttcggccca aaggcatccg gcagatagtc acgcagcgcg tgtgcctcgc ggcccggcag
     1561 atgaatacgc agatccagac cgctgttcag ttcattcata aactgacggc agtgaccaca
     1621 aggcgtatag ttaacggtga tggctgcaag cgctttttca ccactcaacc aggcgtggct
     1681 gatcgcgctt tgttcggcat gaacggtttg ctgcattgtt gcaccaataa attccatatt
     1741 ggcaccgaaa taccaggttc cgctcacacc gcgcgcaatt gcgccaacat taaaattcga
     1801 caatggcgta cgcgcacagg ccgccgccag tggaagtagt gcgaatgcca gcgcgtcttc
     1861 gtccagcccc gttgcgctct tcagcgatga gacttgctcc ccggtcaaca aagcggggaa
     1921 gtacttgtct gccagaatag gttccagtgc agattgcaag ttatccgcaa gttgggcaaa
     1981 agcggtttga aaacgtggat gcatggcgtt gcctcataat catgtaatgg gataacagtt
     2041 tacgaacccg ttatggcttt atatgtgatc tgaatctcat taattatgca acgcgtgaaa
     2101 cttacatcaa aaatgcgcga cgcatcgcaa attttagccc attactgcca gaataatcgg
     2161 gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat aacaccagcg ccagcgaact
     2221 aaatgcacct tcctgataat ccagctcggc gcagcgcgcc gtaccgaggg cgtgcgaggc
     2281 tgtccccatt gccagaccgc gcgcagcttt ggtacgaata cgcatcgcat taagcaatgt
     2341 atggccaaat accgcgccga ggatgccgac gaaaatcacg caaactgcgc tgattgccgg
     2401 aataccgcca atactgccgc caaccgccat tgcaataggc gtggtgactg atttcggcag
     2461 gattgacgcg gcgatttccg gtgaagcgcc catcaacaat gccacggaag tccccgtcac
     2521 catcgcaacc acgctgccga taaaacagat ggtgatgatc gatttccagc gcgcgcggat
     2581 ctggtgtagc tgctcatata aaggataggc cagcgcgacg accgccggtt gcagcagatc
     2641 gtttagcact tcgctacctt taaagtagct gtcgtaagag atgccggtca gcattaaaaa
     2701 aggaatgatc accaccattg ctaccagcaa cgggttaagc aggggaaact tataccgtgc
     2761 cgccagtttg cgggcggcaa aaaagacaat caacgtcagc ggtaatgacc accagatatt
     2821 cgccatcatt cttctgatcc tttctgacct acgactttac gttcaccgtg taccagttgt
     2881 gaactccagc tcaccaccag aaaaaccacc agcgtactga ctgcacagga aaccactacc
     2941 gggccaaact gtgcgcggag caaatcaaaa tattgcatga cgcctacgcc aatcggcaca
     3001 aacaatagcg ccatatagcg aatcagtacg tagcaccccg gattgaccca ttttgccgga
     3061 agaatttgca aggccagcag gacaaacagg atcagcatcc cgatgatgct gcccggaatg
     3121 gttaccggta gcagggaagc aatgaaaatg cctgcataca ggcaggcata aatcaggacg
     3181 aaagcgcgta aatattgcca gataatgttc agtgtcttac tcataatcaa caccccagat
     3241 gagatggatt catcatacaa ttaattcgta aaacgtgcta ccgatcacat taagaaatca
     3301 gcgcttattt tttcgctccc cggcattacg caatgcgcaa cgaactcagg catacatcaa
     3361 gcatagagag ggattacagt ttgcagcaat ccctctttgc agacctttaa tccgttgacc
     3421 ataggcgcta acatagcgcc tcattttttt ccgggtgaag atatgcgtgt gttactggct
     3481 ccgatggagg gtgtgcttga ctctctggtg cgagaattgc tgaccgaagt taacgactac
     3541 gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac tgctgccggt aaaagtcttt
     3601 catcgcattt gccctgagct acaaaacgcc agccggacac catccggtac gctggtgcgc
     3661 gttcagttgt taggtcagtt cccacaatgg ctggcagaga acgccgcccg tgcggtggaa
     3721 ttaggttcct ggggcgtgga tctcaattgc ggctgcccgt cgaaagcggt taacggcagc
     3781 ggcggcgggg cgacgttact caaagatcct gaactcatct accagggtgc aaaagcgatg
     3841 cgtgaagctg taccggcgca tttgcccgtc agcgtgaaag tgcgtctggg ctgggacagc
     3901 ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg ctggtgctac ggagctggtg
     3961 gtgcatggac ggacaaaaga gcagggttac cgcgcggagc atattgactg gcaggcgatt
     4021 ggcgagattc gccagcggct gaatattccg gtgattgcca acggcgaaat ctgggactgg
     4081 cagagcgcgc aacaatgcat tgcgatcagc ggctgcgacg cagtgatgat tggtcgcggg
     4141 gcgctcaata ttcccaacct gagccgggta gtaaaatata acgaaccgcg aatgccgtgg
     4201 ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg aaaagcaggg cgataccggg
     4261 ttatatcacg ttgcgcggat taaacagtgg ttgagttatt tgcgtaaaga atacgatgaa
     4321 gcaacggaat tatttcagca tgttcgggtg ttgaataatt cccctgatat tgcaagggct
     4381 attcaggcaa ttgatatcga gaaactctaa aaaaccatag tgatgttttt tgacaatgtg
     4441 ttagctcttt atcaataggc cgcacggttg tttggtggcg tcaaaattg
//
LOCUS       DAZRUF010000273         4481 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZRUF010000273 DAZRUF010000000
VERSION     DAZRUF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.275"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(8..1069)
                     /locus_tag="ACHXOL_003818"
     CDS             complement(8..1069)
                     /locus_tag="ACHXOL_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR6810678.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1066..2463)
                     /locus_tag="ACHXOL_003819"
     CDS             complement(1066..2463)
                     /locus_tag="ACHXOL_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR6810679.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2618..3616
                     /locus_tag="ACHXOL_003820"
     CDS             2618..3616
                     /locus_tag="ACHXOL_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR6810680.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3727..>4481)
                     /gene="ompG"
                     /locus_tag="ACHXOL_003821"
     CDS             complement(3727..>4481)
                     /gene="ompG"
                     /locus_tag="ACHXOL_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR6810681.1"
                     /translation="EPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVH
                     YQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWL
                     SMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFST
                     QEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGL
                     SVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1227 c         1099 g         1063 t
ORIGIN      
        1 tattgcatta tttttcgctg ggtgttttgc ctttttgcag tgtttctttc acctgaccga
       61 taagctgacg acggaaatcc ccgaggcgag gtttgtcatc gtcaatccac ggcagcgggc
      121 ggcaaagctc catagcttta atcccgagtc gtgccgtcag aagtcctgca ccaatcccct
      181 gagctgcgcg ggtagacaaa cgagcagcga gatcttgcga catccagtcc atccccactt
      241 cgcgcaccag ttcgctggct ccggcaaaag cgatattcag caataccagc ttaaacagac
      301 gcaaacggct gtaatacccc agttcaatgc catacagcgt ggcgatgcga ttaattaaac
      361 gcagattgcg ccaggcgata aacgccatat cgaccaacgc cagcgggctg accgcaatca
      421 tcaacgttga ttccgccgcc gaacggctga tttcgcgccg cgcctgggca tctaaaactg
      481 gctggaccaa atgcgcatac aggctgacca cttcacggtc gttttgcgtt tcatggattg
      541 aggcatacca gcgttgcagc gccggatgcg actgatcaat acccgcctgc tgcgccagtt
      601 tttcgcaaaa tgcgcggcct ttgcccgtgc cgtggctatg caataaatca cgcgcttcgt
      661 cgcgttcatg ggcgcgctgt cgcaagcacc ataagcactg ccactctgtt accacagaac
      721 ctacgccagc gccgataatc aatgccccag cggcacatcc acccagcgct acccagtcct
      781 gggtttgcca ggcattcatt gtccactgta caccctgccc gacaacactt gcgccaaaca
      841 gagccagccc gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcat
      901 ccattaccgc ttcaacttgc ccctcttctt caggcgcttc atcgagcgtg gccggggcaa
      961 aattttgcgc ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgatcga
     1021 cctccagcgg accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc
     1081 tcctattaaa aattccagcg cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc
     1141 catcacctgc gggcgaaacg cttcaaactg gaacccttgc ttgtcccaga acgcctgacc
     1201 gggcaaacgc gcaggaactt caccggggta aacggtgaga ggcgcaccat cgctgagccg
     1261 attaccgcgc aatgccggaa ttttctcgcc gttgacatca ataatgccgc tggtggtcgc
     1321 ctgcaccgag gccagcccca ggcaatccat actaatccct tcaaacgccg cattttgcca
     1381 ggcatcctga atcagttgtt gcagtaatga aaccatattg gcgtgctgat cgaccgttac
     1441 atggtccgct ttcgttgcgg caaacagcag tttgtcgata accggcgaga ataagcggcg
     1501 aaagagcgtg cgctgcccgt agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat
     1561 atcattaaat gcctgtggcc cactgttgag aggttgcagg caatccacca gcacaatctg
     1621 gcggtcaaag cgcagaaaat gattcttata gaaccccttc accaccttct cgcagtaata
     1681 attaaaccgc tcgcgcagca ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga
     1741 ctcgccccag gtatcgacat ccggccacgg gaagaattgc agcgcgggcg cacctgccat
     1801 atctcctggc aagacaaagc gccctggctg aataaagtgc agcccctgct gtttacagtg
     1861 gtggagataa tcggtccacg cggcggcaat gtctgccagc cggttttcgt cggcaggtgc
     1921 tagcgggtcc agcccttcgc acatcattcg ccatttgacc gaccattcgc cgcgctgacc
     1981 attgagtaag cccgtcatct ggcgtgacca gcttaaatag tcctgcgcca gcatcggcag
     2041 gtcgagcaac cattcgccgg gataatccac aatctccaga tacagcgtgg aggtgtcttt
     2101 gaagtggcgt agcagcgaat cgttcgattt aaaacgtagc gccaggcgga tttcactgac
     2161 gccgcgcgta ggcgtcggcc aggcgggagg atcgccatat aactgcgcca gtccttcatc
     2221 ataggtgaag cgcggaatgc caaagtcacg ctgaggaatg cgttttacgc ccagcaggcg
     2281 ctcttcacgc accgcgctta acagcggcaa gcgtgctccg gcgtgaatat tgagcaactg
     2341 attgaccatc gcagtgataa acgctgtttt gccgctgcgg cttagtccgg ttacggcgag
     2401 gcgcagatgt ctgtcgacac cccgattcac cagcgcatta agttcatttt taagtcgctt
     2461 cattgctgtc ctgtgctgct ctgaagacca gaaaaaaatc attcgtaagg atatgaatgt
     2521 gggggccagg ccattgatta tcaactgccc gtgttgtcat cgacgggcga actccataag
     2581 atgtgcagca ttcacgttcg gacggagttc ctgacctatg tcccctacta tttatgatat
     2641 tgccagggtt gcaggcgtat caaaatccac cgtatcacgc gtgctgaata agcaaactaa
     2701 tatctccccg gaagcgcgcg aaaaagtgtt acgggccatt gaagaattac agtatcagcc
     2761 aaacaagcta gcccgcgcgc tgacctcttc gggttttgat gccattatgg tgatttctac
     2821 ccgttcgacc aaaactacgg cgggtaatcc gtttttctct gaagttttgc atgccatcac
     2881 cgccaaagct gaagaagaag gtttcgacgt gatattgcag acgtcgcaca acccggcaga
     2941 agacttacaa aaatgtgaaa gcaaaattaa gcagaaaatg attaaaggca ttattatgct
     3001 aagttcgcca gcggatgagt cattttttgc ccaactcgat aaatacgata ttccggtggt
     3061 ggtgattggc aaagttgaag gtcaatatgc ccatgtttat tctgtcgata ccgataattt
     3121 tggcgacagc attgcgttga ccgatgcatt aattgaaagt gggcatcaaa atattgcctg
     3181 cctgcatgct ccgcttgatg tccatgtttc agtggatcgg gtaaatggtt ataagcagag
     3241 cctggctgcg cataatattg cagtgcgtga tgaatggatt gttgacggtg gttataccca
     3301 tgaaacagca ctgcaagccg cgagagaatt attaagccag tcaccgttgc ctgaggcggt
     3361 gtttgccact gacagtctga aattaatgag tatttatcgt gcggcagcag agaaaaatat
     3421 tgctattccg cagcagttag cggtggtggg ttatagtaat gaaacgctgt cgtttatatt
     3481 aacgcctgca ccgggcggca tcgatgttcc gacgcaggag ttaggtcaac aaagctgcga
     3541 gttattattc cgcttaattt ctggaaaacc gtcaccacag aatattaccg ttgccacgca
     3601 tatgacgctg aaataaatac cagctacctt gcctgaatgc cggatgcgat gctagcgcat
     3661 cttatccggc ctacatttat agatcggata aagtactcgc gccgcatccg gcaatttagc
     3721 ccattatcag aacgagtaat ttacgccgac acctgcataa tggaatttat cactgtctcc
     3781 ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa acggaaagtc cgttctggaa
     3841 atcataaccg taaaataaac ctacacggtt aaaatcatgg ccttcacgtt caatatcatc
     3901 ctgccagtcc cagttactcc agcgatccag cccaatgcgc gtatacggcg tcaccgagtg
     3961 gttgccgagc gtcagcggca aataggcgcg aatttcttgc gtggaaaact caccgttatt
     4021 gcggctgtcg tccatattga agccgcgctc gagataatag ttcactcgca aggcaaccgt
     4081 ttcgttgaag gtatattgca gacctgtttc cgtttcgaca cgggtatcag cgtaaccggt
     4141 agtgttcaga tcgttggcaa atttatacat cgacaaccaa ccgttgaaac gtaaatcgtc
     4201 agtcagtttc acatcccagt ctggcgcgat tttccagcgc tgcatattcg ccgtgtcttt
     4261 acccggttca tcaacgtagt gataaccata attacggaaa ccgccggtca ggccgaaact
     4321 gaaatcatcg ttttcgagga actgataatg cacctccagc tccgggcgat caaaccacgt
     4381 tccacgttta cccgcgctat aatctaccgg cccttcctga taataggcca gagcaattct
     4441 ccacggcccg ttggcggcat taaaatagac tgaaggctcc g
//
LOCUS       DAZRUF010000274         4480 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZRUF010000274 DAZRUF010000000
VERSION     DAZRUF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.276"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..461
                     /locus_tag="ACHXOL_003822"
     CDS             <1..461
                     /locus_tag="ACHXOL_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR6810682.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
     gene            complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHXOL_003823"
     CDS             complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHXOL_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR6810683.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(2231..>4480)
                     /locus_tag="ACHXOL_003824"
     CDS             complement(2231..>4480)
                     /locus_tag="ACHXOL_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR6810684.1"
                     /translation="KLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVSVGKT
                     TSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMSGFGR
                     VNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEGINAL
                     AGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGSIGVM
                     AAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSARTYE
                     NKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSAQNRS
                     DALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPFAPSG
                     QQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDDKEGG
                     FNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAETWQAG
                     FNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDWEGIS
                     DEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTYFGAG
                     DMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQDLPQ
                     IPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSARGRT
                     WIFGGDIRF"
BASE COUNT         1137 a         1048 c         1045 g         1250 t
ORIGIN      
        1 aacaaaatgg gttggctaaa gctctgagag agattggccg tatagaacga acactgttta
       61 tgctggactg gtttcgggat ccggcattgc ggcgacgtgt ccaggccggg ctgaacaaag
      121 gagaagcccg taatgctctg gcccgtgctg tattcatgca tcgattgggg gagattagag
      181 atcggaaacc ggaaaatcaa agttatcgcg ccagtggatt gactttattg acggcggcta
      241 tttccttatg gaacacagtt tacatggaaa gagcggttga tgctctgaag cgtaagggca
      301 tgaaaataaa tgctcaacta ttatcgcatt tatcaccatt aggttgggaa catatcaatc
      361 tgacaggcga ttatatctgg aaaaacagcc ggataccgac ttcaggtaag tttcgtcgtt
      421 tgagatcagt taagatcgac aaggtcaaaa aacaacctta acgtacaata attttcgata
      481 tccaaactga ccccaagtat aaaaaacagc ctgtcacggt ataaaaatcg accatgcaga
      541 aattatgctg gttattttac cgctgctggt ccctcattca gtgactgccc caggggtttt
      601 catgctctcc ttatagtaca ataatttcca tatccaaact cacccctact ttgactttta
      661 tcatagtcaa aacctcttaa cgtgagtttt cgttccactg agcgtcagcc ctctaaaact
      721 actgaataat cagttagaaa acaaacaatc ggaaaaatct ggggttccgg cctagaaccc
      781 catgaacagg catagctgtg atttcattta attattggca caaatcagtt gatcgtacaa
      841 tgctgtgcct gtctcgtttt ctggagattc ctcagcctca tggagcggcg taacaggcgt
      901 accggcagga cagagatgtt tgcttttcct agtccggcca gtttagtgga ataacggagt
      961 ttttatccca tgttttttcc ccccgatcac gactttttca aatcaatgct cagaatacgt
     1021 tctttagctc cttccacaaa cacatcagga tcccaatcgc ggttaaagcc ttctttgatg
     1081 ccaatgtaaa gggtatccat atcgtccgac aacaccatct ggtttgggta gaccggcatg
     1141 ttaaattcac gggtgatgct gtatttggtg gcatccacta cgctgatggt gccgctagtg
     1201 aagttggtga catacagttc gttatatttc ggattgtatt tcaccgcgtt agctccggct
     1261 cctgtgtgca catagccaag ctgtttaccg tcgcgtaagt caaaaatata gagtccatct
     1321 tttgtaccgg aacgatcgtc atggtcgaat accaccacaa aggcacggtc agaagcggag
     1381 tccaccgcca gattctgcac acttgtgatt tgtggtctcg gatctttcaa cgggatggta
     1441 cgcagcagcg tctgattttt cgcatcaatt tcgttgatgt aatctcttcc gccaacgtag
     1501 attttctgtg ttttttcatc catcgtcaga ccatacgctg tacctttgta agcatcctta
     1561 acctcgcctt ttaaggcaag cgtcgtcccg tcgagcatta acagcttatg cagaggcttc
     1621 atgccctcgg ccgttttcag catattgcta taactcacaa acagggtatc ggaatcctgg
     1681 ctgtaaacca tatggcgaaa atgctcaaaa cgcgaatctg cagcgtttgc cgctttaaaa
     1741 cttaacctgt cgctggttct taccagtttg ccggttgggg tgtcaaacat actgatacgc
     1801 agggacgctg actgggtgtg tccgatatag agctgatgtt tatcctgatt tatcgccagc
     1861 gaaaatgccc ggtatggcat cgtgtattta ttttcgactt tcagcgaatc tgaattaaac
     1921 gaatacagat agcctttatt ggcttcacga tttacacgat cggtgactgc tgcaaacacc
     1981 tttttactgg cattatcaaa cgccagttca tagacgcctt catcagggga ctgaaggtaa
     2041 ttcgttttca gggcaaatcg cgaaagttcg cgttcaggtg gatcgatagc catggccatc
     2101 gtgggtaaaa atagtgaaat taacaggtat agcggtgctt tggataaagt gaaaatattc
     2161 atttttcgtt cctgcaataa aattgacata agagagaaaa cgtattgtct gaacctagcg
     2221 gaaattgtca tcagaaacga atgtccccgc caaatatcca tgttctgcca cgcgcggaat
     2281 tggctgggtg ggtctcgtca ccaagacctg gcatcatgtt gagcttattc agcgcctccg
     2341 aataatctct gttcatcagg ttttgtaccg aaagtttcag tgttaggttg cggttgtact
     2401 cgtaagtacc atagagatcg ataatcgttg ggatctgcgg caaatcctgt ttgattattg
     2461 cgccggtatg ttcgtcctgc tcaaaatcag gcgacaggcg acgagccttg cctgtgtatt
     2521 ttatgatagt gcccagggtc aacgcgttat cgaagaagcg aacaccagta tccagcgtca
     2581 tgtattttct gggcagttcg gtcatatccc ctgcgccaaa gtaggtgctg gcgatggagg
     2641 ttggctggtc tgtttgctgc tggctgaaag agagtcggcc aaaagcaaaa cctgcatcat
     2701 aatccatctc cagttcgaag ccctttgcga taacgtcgct tgccgagtta acgtagatgt
     2761 acatattgtc gctgtattca tcgctaatgc cctcccagtc attgccaatc acctcaggca
     2821 tactgcattt acgacctcca gaacaaacca gataagactc gctgtagatg tagttctgga
     2881 tcctgctgcg gtacgccaga gccttaaagc gcagggcatc ctgttcgacc agtaaatctc
     2941 tggtatcaat gttaaaaccc gcctgccagg tttctgcacg ttcaggcttc aggaatgggt
     3001 tcatggatgc gcctcctgaa ttagagaaga acatctcctg gatgttcggg gcgcgcatgg
     3061 atttgctgta gccaatgaac ggctgaagcc atggtgttac ctgagcagaa agctgaactg
     3121 aagggttgaa gccaccctct ttatcgtcaa tatcgtagct accctgtggc acgcagataa
     3181 cgcgtgaatc gcaggcgggc ttgtaccctg tgatcctgtt acgtgtgtag ttgagattga
     3241 aatctgcctc ccagatgccg cgcgtaacct tcagcccggt atacagcgct gaaatatcct
     3301 gctggccgga gggtgcgaac ggattgttct cgatcgattc ctgattcgct ttcgggtcgc
     3361 ccgccgctga gtgaatggtc cggtcatagc gggtacgcat cagtttgctg cccagcataa
     3421 actccaggtc attgtccgcg acagtgaacc ggctggtatt gttgatatcc agcgcgtcag
     3481 aacgattttg cgctgaggtt ttgtagaaag tatacagcga gccatcacga tacttttgat
     3541 taccgcgact ggtactggcc gttacgttaa agtcaattaa ttcagaaaaa ggggtgtaat
     3601 gatatttaat gtaatagtcg tcactggtta tatcacgacg tgtaaattta ttttcatagg
     3661 ttcgagcgga aagttcgaag ctgttaaatt cgtctggtct gatatccatt ttatacagtt
     3721 gggatttggg gttctgcttc atatatttat cataaccaaa ctctttgctg ttaattccag
     3781 aaccatttga gaaattagag tacacagaac tgccgctcac agcagccata acgccaatgc
     3841 ttcccgtatc cgtaaaagcg tcgctttttc caccaagggc gatcattccg ctgcgtccca
     3901 gcccattact accgacagag aaacgtgaac gaaggccata tgtattaccg ttaaatatta
     3961 catcgtcaac gccaatagta cgcatatttg cactaccggc aagggcgttg atcccttcag
     4021 agccactgct gtcaccgcgt gtaacatcaa ctgctaccag taagttagga tctataagta
     4081 cgccagccat attgttagtt gaaccatgcg tcgttgttcc ggaggtagag gttccgtaaa
     4141 aactctgggt aataccatcg accatagtgt ttacacgacc aaatccgctc atgcctcgaa
     4201 tattcacact gattgctccc tgaccaggat ctatttgagt ataagtacca ggcatactac
     4261 gcactgttgc gtcaagtgat tgcaggtttt tgtccgttgt ccgggaactg gtcgcgcctg
     4321 ttttctcgag agcttcctgc tcactggtcg tcttcccgac agaaacgctg agcggactga
     4381 attctgccgc tccttttttg tcggaattag ttttttcact gatggattgt gagtaagcac
     4441 cgcagctgag caataatatg cctgagccga tagccagttt
//
LOCUS       DAZRUF010000275         4457 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZRUF010000275 DAZRUF010000000
VERSION     DAZRUF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.278"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..264)
                     /locus_tag="ACHXOL_003825"
     CDS             complement(<1..264)
                     /locus_tag="ACHXOL_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR6810685.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAP"
     gene            493..1101
                     /locus_tag="ACHXOL_003826"
     CDS             493..1101
                     /locus_tag="ACHXOL_003826"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR6810686.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1199..2590
                     /gene="selA"
                     /locus_tag="ACHXOL_003827"
     CDS             1199..2590
                     /gene="selA"
                     /locus_tag="ACHXOL_003827"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR6810687.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEIIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2587..4431
                     /gene="selB"
                     /locus_tag="ACHXOL_003828"
     CDS             2587..4431
                     /gene="selB"
                     /locus_tag="ACHXOL_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR6810688.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          996 a         1135 c         1322 g         1004 t
ORIGIN      
        1 cggcgcgggc gtttttgtcc ggtaactgct gtaggtgcgg gagaggatga acggcagcgg
       61 ggcgggcagg gcgatgtcgg tttcaccggg aaggaccttt gcaccgagca ggggattgac
      121 cggatggccg gacgtcactc cgccggggca caccgaacag gccacaccgg tgggggcacc
      181 gatgcgcacc ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcgccctg
      241 acgcgctgcc ggttttccgc tcatctgctc atccttgttt tatcgggtta tttgtctgct
      301 tccaggcaaa accctacaga tttataagaa agccttaagt gagatatcca tcagatagaa
      361 acaattagga aaatatttat atcacaatga taaatatgtg atttatttgg aagtaacaaa
      421 ccatcaacat tttctgccct ttttcccatc accaggccat actgatattt ttcctcgcac
      481 aaaggactcg gcatgaaact cgtcggtagc tacaccagcc cgtttgtacg caaactttct
      541 attctgttgt tagaaaaggg cataactttc gaatttatta atgaactgcc ctataacgcg
      601 gacaacggcg tggcgcaatt taacccgtta ggaaaagtgc cggtgctggt gaccgaagag
      661 ggcgaatgct ggtttgattc gccgatcatc gctgaatata ttgagttaat gaatgtcgct
      721 ccggcgatgt tgccgcgcga tccgctggag tcgttgcggg tgcgcaaaat tgaggcgctg
      781 gcggatggca ttatggatgc cgggctggta tcggtgcgtg aacaggcgcg tccggcggcg
      841 cagcagtctg aagatgaatt gttacgccag cgggagaaaa tcaaccgcag tctggatgtg
      901 ctggaaggat atctggtcga tggcacactc aaaaccgata cggtcaatct ggcgactatc
      961 gccattgcct gtgctgtcgg atatctcaat ttccgccgcg ttgcgccagg ttggtgtgtc
     1021 gaccgcccac acttagtcaa actggtcgaa aacctgttta gccgcgaaag ttttgctcgc
     1081 accgaaccgc caaaggcttg atgcgcgata tgtcctcctg acccatctca cgttacaatc
     1141 cgtggttatg ttaaacgccc ttctccgtgt gagagggcct tgatcagcca ggtttcctat
     1201 gacaaccgaa acgcgttccc tctatagtca acttccggct attgatcgct tattgcgcga
     1261 tagctccttc ctttctttgc gtgatactta tggtcacacc cgcgtggtgg aattgttgcg
     1321 tcagatgctc gacgaagcgc gagaagtgat tcgtgacagc cagacgcttc ctgcgtggtg
     1381 tgaaaactgg gcgcaagaag tcgatgcccg gttgacgaaa gaggcgcaga gcgcgctgcg
     1441 tccggtgatc aacctgacgg gaaccgtgct gcataccaac cttggacgag ctttacaggc
     1501 ggaagccgcg gtggaagccg ttgcgcaggc tatgcgttcg ccagtgaccc tcgagtacga
     1561 tctggacgac gccggacgcg gacatcgcga tcgggcgctg gcgcaactgc tgtgccgtat
     1621 tacgggggcg gaagatgcct gtatcgtcaa taacaatgcg gcggcggtgt tattgatgtt
     1681 ggcggccact gccagcggga aagaggtggt agtatctcgc ggcgaattgg tggagattgg
     1741 cggcgcgttt cgtattcccg atgttatgcg tcaggcaggc tgcaccctac acgaagtagg
     1801 gaccaccaac cgcacgcacg cgaatgatta tcgtcaggcg gtgaatgaaa ataccgcact
     1861 gttgatgaaa gtacatacca gtaactacag cattcagggg ttcaccaaag cgatagatga
     1921 agcggaactg gtggcgctcg gcaaagagct ggatgttccc gtagtgactg atttaggcag
     1981 tggctcgctg gtcgatctta gccagtacgg tttgccgaaa gagccaatgc cgcaggagtt
     2041 gattgcggcg ggcgtcagtc tggtgagctt ctccggcgac aagttgttag gcgggccgca
     2101 ggcagggatt atcgttggta aaaaagagat aatcgcccgc ctgcaaagcc acccgctgaa
     2161 gcgtgcatta cgcgcggata aaatgaccct cgcggcgctg gaagccacgt tgcgtcttta
     2221 tttacaccct gaagctctga gtgaaaaatt accgaccctg cgcctgctta cccgcagcgc
     2281 agaggtcatt caaatccagg cacaacgttt acaggctccc cttgccgcac attacggcgc
     2341 ggagtttgcg gtacaggtta tgccatgtct ttcgcagatt ggcagtggtt cgctgccggt
     2401 tgatcgcctg ccgagcgcgg cattaacgtt tacaccccat gatggacgcg gtagccacct
     2461 tgagtcatta gccgcccgct ggcgtgaatt gccagtgccg atgattggtc gtatttatga
     2521 cggacgattg tggctggatt tacgctgcct tgaagatgag caacggtttt tggagatgtt
     2581 gttgaaatga ttattgcgac tgccggacac gttgaccacg gcaaaacaac cttattgcag
     2641 gtgattactg gcgtaaatgc tgaccgtctg ccggaagaaa aaaagcgcgg catgaccatc
     2701 gatctcggct atgcctactg gccgcagccg gatggtcgcg tgcctagttt tatcgacgta
     2761 cccggtcatg aaaagtttct ttccaacatg ctggcgggcg ttggtggtat cgatcacgcg
     2821 ctgttggtgg tggcgtgcga tgacggcgtg atggcgcaga cccgtgagca tctggcgatt
     2881 ttgcagctga ccggtaaccc gatgctgaca gtggcgctga ccaaagccga tcgcgtggac
     2941 gaagcgcgtg ttgatgaggt tgaacgccag gtaaaggagg tgctgcggga atacggtttt
     3001 gctgaggcaa aactgtttat caccgcagca accgaaggtc ggggaattga tgccctgcgc
     3061 gagcatctgc ttcagttacc aggacgtgaa cacgccagcc aacatagtgt ccgcctcgcg
     3121 atcgaccgcg catttaccgt aaaaggtgcc gggctggtcg tcaccggtac ggcgttaagc
     3181 ggggaagtga aggtaggcga ttcactctgg ctgactggtg taaataaacc gatgcgtgta
     3241 cgtgcgctgc atgcacaaaa ccagccaaca gaaaccgccc atgccgggca gcgtatcgcg
     3301 cttaacatcg cgggtgatgc ggaaaaagag cagattaacc gtggcgactg gctgcttgcc
     3361 gatgcgccgc cagagccgtt cacacgggtg attgtcgagc ttcaaaccca tacgccgctg
     3421 acccagtggc agccgctgca tattcaccac gccgccagcc acgttacggg acgcgtttcg
     3481 ctgctggaag ataaccttgc cgagctggtg ttcgacacgc cgctgtggct ggcggataac
     3541 gatcgcttag tgctgcgcga tatctcggcc cgcaacacgc tggcgggggc gcgtgtggtg
     3601 atgcttaacc cgccgcgtcg cggtaaacgt aagccggaat atctgcaatg gctggcgtct
     3661 cttgcacggg cgcagagcga tgccgatgcg ttatctgttc atctggaacg cggcgcggtt
     3721 aaccttgcgg atttcgcctg ggcgcgccag ctcaacggcg aagggatgcg cgaattgctg
     3781 caacagcctg gctatattca ggctggttat agcttgttga atgcgccggt tgccgcccgc
     3841 tggcagcgga aaattctcga cacattagcg acttatcatg agcaacatcg cgatgaacct
     3901 ggtcctgggc gcgaacgtct gcgacgtatg gcgttgccaa tggaagatga agcgctggta
     3961 ctgttgctga ttgaaaagat gcgcgaaagc ggcgacatcc tcagtcatca cggttggctg
     4021 catctgccgg atcacaaagc gggcttcagc gaagagcagc aggccatctg gcaaaaagca
     4081 gagccgctgt ttggtgacga gccgtggtgg gtgcgtgatc tggcaaaaga gacgggtacc
     4141 gacgagcagg caatgcgcct gacgctacgc caggcggcgc agcaaggaat aattaccgcg
     4201 atcgtcaaag atcgttatta ccgtaacgat cggattgtcg aatttgccaa tatgatccgc
     4261 gatctcgatc aggagtgtgg ttcaacctgc gcggcggatt tccgcgatcg cttaggcgta
     4321 ggccgaaagc tggcaattca gattctggaa tattttgacc gcattggctt tacgcgtcgt
     4381 cgtggaaatg atcatttatt gcgcgacgca ttattatttc cggaaaaata aggaaatgat
     4441 taattaattt ttaaaat
//
LOCUS       DAZRUF010000276         4444 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZRUF010000276 DAZRUF010000000
VERSION     DAZRUF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.279"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(17..3121)
                     /gene="acrF"
                     /locus_tag="ACHXOL_003829"
                     /pseudo
     CDS             complement(17..3121)
                     /gene="acrF"
                     /locus_tag="ACHXOL_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(3133..4290)
                     /gene="acrE"
                     /locus_tag="ACHXOL_003830"
     CDS             complement(3133..4290)
                     /gene="acrE"
                     /locus_tag="ACHXOL_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR6810689.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         1103 a         1182 c         1085 g         1074 t
ORIGIN      
        1 cctttatgtt tctgatttat cctttaaagc aacggcggat caccacaaag aacaccggta
       61 cgaagaagat tgccagcaac gttgcagaga ccattcctcc cattaccccg atacccactg
      121 cgttctgcgc gccactgccg gcaccgttac tgatagctag cggtaatacg ccgagaataa
      181 aggcgagaga ggtcatcagg ataggacgca gacgcatacg tactgccatc agtgtcgctt
      241 caacaacacc tttacccttt ttctccatga gatctttagc gaactcaaca atcaaaatag
      301 cgtttttggc cgacaagcca attgtcgtta gcaagcccac cataaagtag acgtcatttt
      361 tttgattaaa gagtgttgcc gccagtagca cgccgacaat ccctaacggc acgaccaaca
      421 taaccgagac aggaattgac cagctttcat agagtgcagc aaggcacagg aagacaacca
      481 caaaggaaat tgctaccaga gcgggagcct ggtttcccga taagcgttcc tgatacgaca
      541 tacctgtcca gtcataacca atgcccgcag gtaattttga cgcaaggttt tccatcaatg
      601 ccatggcatc gccggaactg gttcctggcg cggcttcccc ctgaatctcc attgacggca
      661 gaccgttgta gcgttccagt cgcggagagc catacaccca atgtgaagtg gtaaaggcag
      721 agaatggcac catttcgccg ttggcgctgc ggacataaag tttatcgaca tcttctggca
      781 gcatacggaa tttggcatcc gcctgaacat acaccttttt cacgcgacca cggtcgatga
      841 agtcgttaac gtaagtccca cccagcgccg ttgaaatggt ctgattgatg tcagaaagtg
      901 aaacacctaa tgcctgcgcc ttttcctggt caacttccag tttaaactgc gcggtgtctt
      961 ccagaccatt aggacgcacg ctgactaagc tggcaggatg ttgcgccgcc atgccaagca
     1021 actggttacg ggcctgggtc agggcatcgt gacccagccc agcctgatca attaactcaa
     1081 agtcgaaacc cgttgccgtg cccagttcaa caatggctgg catattgaat ggaatgacaa
     1141 aaccgtcgcg gatcttgccc aattccattt tggcacgatg gattaccgct tccgcactgt
     1201 tttcgtcacc actacgctct tcccacggtt tcagactgac gaaagccata ccggcgtttt
     1261 gtgcctggcc gctgaagcta aagccgttaa ccgtaaagac actttctacg ttcgctttct
     1321 cgttattcag atagtaatcc gtcacttgat ccaacacttt ttgcgtccgc tcttgcgtcg
     1381 cgccagcggg taactgaatc atggtcagaa agacaccctg atcctcttca ggtaagaagg
     1441 aagacggaag acgtaaaaac aacaccacca ttcctgcaac aatcagcgca tagatcagta
     1501 aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt ggtgtagtgg ttaacgctat
     1561 gatcgaaggt ggtattaaac cagccgaaga aaccgccctt attttcgtga tgctcagcag
     1621 agacgggttt aagcagcgtt gcacataacg caggggtaag aatcaatgcc accagaacag
     1681 aaagcgccat tgccgaaacg atggtgatag agaactggcg ataaattgcc ccagtagaac
     1741 cgccgaagaa tgccatcgga ataaataccg ctgacagcac catcgcgata cccaccagtg
     1801 cgccctgaat ttgcgacatc gatttttccg tcgcttcttt tggcgggagc ttatcctcca
     1861 tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc atcgacgagc agcccgatgg
     1921 caagcaccat cccgaacatc gttagtgtgt tgatggagta accaaaagcg gcgaggatgg
     1981 caaacgtccc taacaacacc acgggtaccg caatggtggg gatcagcgtt gctcgcatat
     2041 tctgcaagaa cagatacatc accaggaaca ccagcataat ggcttcgaac agcgttttta
     2101 ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc ataagggtag agaaccttca
     2161 ttccctgcgg gaagaatggc tgtaattccg ccagttttgc cttaatggct ttcgcggtat
     2221 cgagagcgtt cgcgccggtt gctagcttaa tccccaggcc cgccgccggt tttccgttga
     2281 tacgagcgat aacgttatag ttttcaccgc caagttcaac tcgtgcgaca tctttcaggc
     2341 gtaccaccga gccgtcactg tttacgcgca gggttacttt gccgaattct tccggatttt
     2401 ttaaccgcgt ctgagcaata atcgaggcgt tcaactgttg ccctggtaac gctggcgttc
     2461 cgcccaactg cccggcagcg atctgatcgt tctgtacctt caactggtta atcacatcaa
     2521 ccggtgtcag tttatatttg tttagcagat cggcgtccag ccagatacgc atcgcatact
     2581 gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg gctaagcgta tctttaacgt
     2641 tagaggccac atagtccgag atatcgtcct gtgtggtgtc tgggttatca gagacaaagc
     2701 ccgccaccat caaatagctg ctactggact tttcaacact gatcccctgc tgctgaacct
     2761 cctgcggcag caacggcgtg gcgagctgga gtttgttctg cacctgcact tgcgcgatat
     2821 caggatcggt cccggactgg aaggtaaggg taattgtcac gctaccggcg gaatcgctgg
     2881 tggaggacat atacatcagg ttatcgatac cattcatatt ctgttcgata acctgcgtca
     2941 ccgtatcctg cacggtctgc gcatcagcac ctggatagtt tgcctaaaca gaaaccgcag
     3001 gcggtgcaat ggttggatac tgagcgacgg gcaattgtag gatcgccagt gcgcccgcca
     3061 tcatcagaat aatggccagc acccatgcaa atatcggtcg tcgaataaaa aagtttgcca
     3121 tgtcagatta ccttacttcg atgcagtatc tgcgggggta tcggtagtgg ctttaacctg
     3181 ctctcccgga cgcgcttttt gcaggccgct gacaatgact tgatcgccag atttcagacc
     3241 ttcgctaatc aaccatttat cgccaatcgc ctgactggca acgacagggc gcgcttccac
     3301 ctgactttta tcgttaacaa tcagcacggt tgcatcacca cgcggtgtgc ggctaacgcc
     3361 ttgttgcggg ataagaatgg cgtcaggttg gacgccttca tcaatccgtg cacgcacaaa
     3421 catacccggc aaaagcgtat gttgcgggtt agggaagaca gcacgtaggg ttatggagcc
     3481 ggtgcattca tcaacggtca catcagagaa ttgcagcgta cctttcaggg gataggtttg
     3541 accgttttcc atgaccaact ctacgttgct ggtggcgttt tccttatgca aatttccttg
     3601 ctctacggat tgcttcagcc tcataaaatc gttgctggat tgggtcacat caacgtagat
     3661 aggatcgagc tgctggacag tcgccagttc agtcgtttgc ccattagtga caagagcgcc
     3721 ttcggtcacg gtcgatttgc cgatacgtcc gctaattggc gcagtcactt tggtataagc
     3781 gagattgatg cgagcgcttt cgactgtggc ttttgcggca atcacggcgg catcggcctg
     3841 gcgagcatca gcaatggcct ggtcgtactc ctgctggctg atgtatttcg tacccacgag
     3901 cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg gcggcttcac ttttcgccag
     3961 ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg ggatcgatct ggtacaggga
     4021 ctggcctgct tgtacatcgc tgccttcagt gaaattacga ttcagtacga tcccgctaac
     4081 ctgtgggcga acttcggcta tacggtaagc attggtgcgg cctggtaatt cagtcttaac
     4141 ttctaacggg gccgttttca caatatgaac ggtaacctgc ggttcaccga cgtgagcttt
     4201 ctcttctccc ttatcgttac aaccgacgat taaagccgcg gagatcagaa taaaggaggg
     4261 caggaggaaa aacctggcat gtttcgtcat tactattcct caaaaaaaca aaagcgcgtt
     4321 atttacccag aaaggcaacg cgttaactcg cagaaagaaa aatacagttt gctatcctac
     4381 aaattatcat tcgtcgatgt aaggaatagt tatgaatact ggcgtctcaa ggcacataaa
     4441 caca
//
LOCUS       DAZRUF010000277         4411 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZRUF010000277 DAZRUF010000000
VERSION     DAZRUF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.280"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHXOL_003831"
     CDS             complement(<1..105)
                     /locus_tag="ACHXOL_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810690.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHXOL_003832"
     CDS             complement(173..2122)
                     /locus_tag="ACHXOL_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR6810691.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHXOL_003833"
     CDS             complement(2207..2542)
                     /locus_tag="ACHXOL_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR6810692.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHXOL_003834"
     CDS             complement(2542..2898)
                     /locus_tag="ACHXOL_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR6810693.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHXOL_003835"
     CDS             complement(2898..4394)
                     /locus_tag="ACHXOL_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR6810694.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1021 a         1314 c          970 g         1106 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agacatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagccttat c
//
LOCUS       DAZRUF010000278         4364 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZRUF010000278 DAZRUF010000000
VERSION     DAZRUF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.281"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHXOL_003836"
     CDS             complement(<1..215)
                     /locus_tag="ACHXOL_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR6810695.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="ACHXOL_003837"
     tRNA            complement(330..405)
                     /locus_tag="ACHXOL_003837"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="ACHXOL_003838"
     tRNA            complement(412..486)
                     /locus_tag="ACHXOL_003838"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="ACHXOL_003839"
     tRNA            complement(603..687)
                     /locus_tag="ACHXOL_003839"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="ACHXOL_003840"
     tRNA            complement(696..771)
                     /locus_tag="ACHXOL_003840"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="ACHXOL_003841"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="ACHXOL_003841"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR6810696.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHXOL_003842"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHXOL_003842"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR6810697.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHXOL_003843"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHXOL_003843"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR6810698.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHXOL_003844"
     rRNA            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHXOL_003844"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1112 a         1095 c          983 g         1174 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aacgcaaaaa
     4201 ggccatccgt caggatggcc ttctgcttaa tttgatgcct ggcagttccc tactctcgca
     4261 tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg
     4321 tcaggtggga ccaccgcgct acggccgcca ggcaaattct gttt
//
LOCUS       DAZRUF010000279         4329 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZRUF010000279 DAZRUF010000000
VERSION     DAZRUF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.282"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..582)
                     /locus_tag="ACHXOL_003845"
     CDS             complement(<1..582)
                     /locus_tag="ACHXOL_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR6810699.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
     gene            complement(645..1094)
                     /locus_tag="ACHXOL_003846"
     CDS             complement(645..1094)
                     /locus_tag="ACHXOL_003846"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR6810700.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1091..4183)
                     /gene="ebgA"
                     /locus_tag="ACHXOL_003847"
                     /pseudo
     CDS             complement(1091..4183)
                     /gene="ebgA"
                     /locus_tag="ACHXOL_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1028 a         1090 c         1168 g         1043 t
ORIGIN      
        1 gaagttcggg atcatcgcgt caacggtgat ggcgtcagca ggctgtacgc cgccaaccag
       61 cgccgtacct gccagtaaaa tgtaggagag cgttaacagc agcatcagcg ttgaagtgac
      121 ggaggtaatt ggccccaaca ttttcgcccc gttggtggaa acccaggtgg agaaggcgaa
      181 cagcaccata ctgataatgg tggtggcaac cggcgtcata atatattcgt atccgaggaa
      241 ggcatacgaa gcataagcaa taacgcgcgg caacagtgag gtgaaaaaga acaggtttac
      301 gaaccagtag gtataggcag taataaatgc ccaacgtccg cccagcgaac tttttaccca
      361 tgcgtagaca ccggcttctg agtttttatt taacgaaaca aattctgcga tgatcagaca
      421 gaagggaata aaataaaaaa tcgtcgcgag gaaaaacatc ggtgccgagg ccagtccaag
      481 atcaatatta ttattgataa cgttgttaaa gctgtaaacg gcggcaaaag tcagcgagag
      541 caagccgaat ttgccgattg tattacgttt ggtatcagac ataacacatc tccgaaaatg
      601 agtagggtag cggtggcggc aaactccttt tgccgccgta gttgttattt gttatggaaa
      661 taaccatctt cgatggtgac tttgagaacc acttttttga ccgcgttatt gcagataaac
      721 cgatacgctt catggatatc gcaaataacg atttgccctt cgtggacctc aacggtttct
      781 ccgcagcctt ttaaatattc acggtcagtt tcatcacgat aatattccac tacctgtaat
      841 gtctctttcg gcgcatattc aattttttgc tgcccttgca ggtagtaatg cacttcaaaa
      901 tagcgtcgat gcccggtaaa tagcgcatcg gtcgcggagt ctgtctccac gcggtaagtc
      961 aatgagtcac cgatggagtg ggcgacgcca ggctgaatgt tgtcgatatt ttcaatcgct
     1021 tcaacgcagc gttgccactt cttgccagag gcgtaaatct ggcggaactg ttctaagtta
     1081 tcgatgatcc tcattgctta ttctcgctgt gcaaattcgt ggaaaagaac cctgcgccga
     1141 actcatacga cgccaggctt tgcgcggtag cttctccgcc agaaaccggc agcaacgtaa
     1201 agccgtagct gaagtcacgg aaccagacgc gccaggagtc cagcacctcg ctgccccagg
     1261 agttggagcc gaggccaagc agctggtgat cgaggttcag ggtgatgtca tcactgcgct
     1321 gcagctcgtt acagtgctgg gcagcgtgga tgttttccta ggtatagtgc caggcgctga
     1381 agttaattgg gcgctgcgga accaccagca gaccgttacc gtggcggtta gtcagtgccg
     1441 tccagcggac atgctgacgg ttaccgttgt tctgcgggaa gggatagttc tcgaacatgg
     1501 catcgacggt gctgcgccag atatcgatga tgttagcctg ctggctgtcg gcgtagtttt
     1561 cgcccggtcc acgaccgtaa tacgccacct gatcgtattc gccgttaatt cccatggtga
     1621 aaccgatgca cggaatgatg tgcggatagt cgccgtaacg ctcgccggaa agcgccacgt
     1681 taacctggcc atcggcagcg atgcgccaga tgtaggtgca gcgcattccg aaatcaaaca
     1741 ccggcggggc aataaccgtg cggctgatga tcaacacttc accctcgctc tgttctacgg
     1801 caaagtcgcg cagatgttcc tgcatgatct gcaaatgatt cggctgccac aacccttcgt
     1861 actcctgctt gtggttgtcg atcatcggct tgaagaagtt gatctttggc tcgcgggtca
     1921 gcagcgactc gccatttacc tgccaggatg tcggtttgcc actcattttt gagaaggtga
     1981 tcgcgaagtt gtagccgcga acggtgcagc tcaaacgatc gtcttccagc gtcagcggac
     2041 gcgcattatt tggtgcgaaa ggcactggct gcgcggtgtt ttccttcagc gggaactgat
     2101 aagtggcgat agaatgcccg gcttcgctgt agcgggtgcg ggaatctttg gtcaccgtaa
     2161 tgttgaggaa cgcttcgcgg gcgtccagct gcggcagcgt gatctgcaag ggggcttcgc
     2221 tgttcggcgc aacgtcgcgc agtttaatct gctgcgtcgc gagcgtttca ccttcggcgc
     2281 gcacctctgc gtgcagggtg tagtcatcaa gcgtggtaaa ccacagttta ttttcgactt
     2341 tcaactcgcc gcgagtcaga tcccgcgcgt ggatttttac cggcgcgata acctgtttgt
     2401 actctttcag gcccggtccc ggcgtctgat cggaatagat caaaccatca agacagaagt
     2461 tatagttgtt gggatagtcg ccgtagtcgc cgccgaattt ataccagaca ttgccgtggt
     2521 cgtcctgtgc ctggatcccg tggtcgcacc actcccagac ataatgaccc tgaatgcaat
     2581 cgtgcttata gaagacgttc tggtactccg tcagcccgcc cggtccgttc cccatcgcat
     2641 gagcatattc acagatgatg cgcggcttcg gatgcgggta ttcaccaaac tcattcatca
     2701 gcggcacgcg ggtgtacatg gtggaaataa tatcgaccac ttcagcatcg cgatcttctt
     2761 cgtaatgcac cagtcgcgtg tcatccagcg ccttcgctgc gtggtacatc gcgcggatgt
     2821 tacagccata gccggattca ttgcccagcg accagatgat gatcgacgga tggtttttct
     2881 gcgcgtgaat atggcgaaca atgcgctcga cgtagacttt ttcccactgc ggatcgtcgg
     2941 taatacggct gatatcgccg acattagcaa agccgtgcga ttcgacgtcg gtttccgcca
     3001 tcacaaacag gccgtagata tcacacagtt cgtaaaaacg cggatcgttc gggtagtgag
     3061 cggtacgcac ggagttgatg ttgtgctgct tcatcaactg gagatctttc tcgacgcgat
     3121 ccattccaac ggcgcggcct ttgcgatgat cgttgtcgtg acggttgacg ccgtgcagca
     3181 tcacataacg gttattgatc cagaacagac cgtcgcgcac tttgatatca cggaagccaa
     3241 cgcgttgtgg taccacttcc agaacgttgc cgttggcgtc tttcagcgtc atgaccagat
     3301 ggtaaagata aggggattct gctgaccatt gctgcggctg ttcgacagta aaagcaaagc
     3361 tggcgctggt cagtttttca attgccaaat gatcaatggc gctgctgtgc accacgcgtt
     3421 cgccatcaaa cagggtatat tccagcgtcg tgacgacagg ggaggcggcg agattttcca
     3481 gcaccacttc gcaggaaagc gtggcatcgc aataggcttc gtcaaagtcg gtacgcacgg
     3541 tgaaatcgtt aatatgtgtt agttgttttc cgatcagata aacatcgcgg aagatccccg
     3601 ctgaccacca catatcctgg tcttccacgt aggtagagtc cgcccattgc atcacgcgca
     3661 cacacaacag gttgtcgccg gttttaacca tcgcgctgat gtcaaactct gcggtcaggc
     3721 gactgccctt gctgaaaccc acatactgac cgttaacgta gacttcaaaa taggtttcga
     3781 cgccgtcaaa tttaatcagc gtctgtttac cctgccagcc gtcgctgagg gtgaaaatac
     3841 gttgataggc accggttggg ttatcgctgg gaacaaacgg cacatcgatg gggaacggaa
     3901 aaccttcgtc ggtatattgc agtttgccgt gaccttccat ttgccacatg gcggggacgg
     3961 taatatgccc ccagtcagcc attaactcag aggtgaaggc ttcaggcact tgcagcgggt
     4021 ggtcaaaaaa gtggaaattc cactgaccgc ttaagggcag aaacaggctg ctggtttcgc
     4081 gggcaaaggt acgcgcttgc gcaacagaat catatgaaaa aaagtacgca cgtggcgcaa
     4141 gtcggttttc gtgggtgagc tgaatgtttt cccagcgatt catagggcct cccggtagag
     4201 agaaatcgga accattcatg gtgatggtag tttgtgtaat tgctggggag tttagtaaaa
     4261 tttttactaa aaagtggcag gtaaatgcat ttactttaac tggatcacga aaataaacgg
     4321 cagcactgt
//
LOCUS       DAZRUF010000280         4312 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZRUF010000280 DAZRUF010000000
VERSION     DAZRUF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.283"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..1873)
                     /gene="speC"
                     /locus_tag="ACHXOL_003848"
     CDS             complement(<1..1873)
                     /gene="speC"
                     /locus_tag="ACHXOL_003848"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR6810701.1"
                     /translation="MKSMNIAASSELVSGLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     "
     gene            complement(2036..2119)
                     /gene="yqgH"
                     /locus_tag="ACHXOL_003849"
     CDS             complement(2036..2119)
                     /gene="yqgH"
                     /locus_tag="ACHXOL_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR6810702.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2272..2979
                     /locus_tag="ACHXOL_003850"
     CDS             2272..2979
                     /locus_tag="ACHXOL_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR6810703.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3085..3160
                     /locus_tag="ACHXOL_003851"
     tRNA            3085..3160
                     /locus_tag="ACHXOL_003851"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3118..3120,aa:Phe,seq:gaa)
     gene            3358..>4312
                     /locus_tag="ACHXOL_003852"
     CDS             3358..>4312
                     /locus_tag="ACHXOL_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR6810704.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1115 a         1142 c         1036 g         1019 t
ORIGIN      
        1 tatgcgcatc ctgggggttc atcaccactg acgggaaact ctgctggcgg aacatcgctt
       61 tttgtaggtc tttgacgtcg aaactgacat acagatcgtg catctcctga cacaactggc
      121 gcagggtgta gtcgcgatag cgcaccggat atttgttata aacgctcggc aacacctcag
      181 ccagcggcga gtcatcctca atatgctgtt caaattgcgc cagcatcgcc accagttgtg
      241 ccagcttctc gtggctttcc gccggagtta ataagaacag aatggagttg agatcgcact
      301 tctccggcac aatgccgttc tcacgcagat agtgcgccag aatcgtcgcc ggaacgccaa
      361 agtcgctata ttcgccggtt tcggcattga tacctggtgt agtgagtaac agcttgcacg
      421 gatcaacaaa atactgatcc gcggcatatc cttcaaagcc gtgccacttc gcccccggct
      481 caaaactgaa aaaacggcgg tcgctggcta acacagatgt cggataatcc tgccacaatt
      541 tgccatcaac aacgggcggg ataaacgggc ggaacagctt acagcgcgca agaatagcct
      601 tgcgcgactc aatacccaac tcaacgcact cagcccacag ccgacgccca ctctcccctt
      661 catgaatttt ggcgttaaca tccagtgcag caaacagcgg atagaaaggg ctggtagaag
      721 catggagcat aaaggcgtta ttcaaccgct tatgcgggca aaaacgcgcc tgtccgcgga
      781 tatggttatc ttttttatgg atctgcgacg tctgtgagaa tcccgcctgc tgtttgtgca
      841 ccgactgagt cacaaagatc cccggatcgt tttcgttaag ttctaatagc agcggcgagc
      901 tatccgccat catcgggata aactgttcgt aaccgaccca cgcggaatca aacagaatgt
      961 aatcacacag atgcccaacg gtatcgatca cctgacgggc gttatagaca gttccgtcat
     1021 aggttcccag ctgaataatc gccaggcgaa acgggcgcgg cagctcggct ttttctggcg
     1081 caacgtcgcg aatttgctgg cgcagatact cttcattaaa acagtgcgca tcaataccgc
     1141 caatgaaacc aaacgggttg cgtgaagctt ccagatagac cgacgtcgcc cccgcctgaa
     1201 tcagcgcgcc gtgatgattc gacttatggt tgttacggtc gaagagcacc agatcgccac
     1261 gcgttaacag cgcattcgtc accactttat tcgctgccga tgtgccgttc agcacaaaat
     1321 aggttttatc ggcatgaaag actttggctg cgaatttctg cgcatccttc gccgatcctt
     1381 catgaataag cagatcgccc aattttacgt cagcgttaca catatcggcg cgaaagacgt
     1441 tctcaccaaa gaaatcgtaa aaatggcgtc cggcaggatg ctttttaaaa aacgcaccat
     1501 gttgatgtcc agggcaagca aaggtgctgt tgcccatctc aacgtactgc gtcagcgtgt
     1561 cataaaacgg tggcagcaaa ttctcttcat actgacaggc tgcggattcc agctccagcc
     1621 actgctgctc gttgccgttg attaccgccg taacgcccgc aggtaattca acagcatgtt
     1681 cggaatacaa aaacaccggt agatgaaaac cggtgcgctt aagcaacgca agaatgccac
     1741 tgcgactatc cgcagcggta atgacgactg ccgcgacatc cgtaaaatca gtatctccca
     1801 acgccaccac gcggcgatga gtagaaagtc cggataccag ttcactactg gcggcaatat
     1861 tcattgattt cataagcgca aaacccgttt cggggaagta agaataccgg acaaagtgta
     1921 aaaccctgcc ccatgagata tgggtcaaac tggtcaccag ctccgaccgc cagacatcag
     1981 taaaagcaga aacgctctga ttttactgtt gtcctgcagt gaacgtgcgt taacttcacc
     2041 gcatgagcag taacatagaa agggaaacgt ttcgcgcgaa acggcgataa gcgagagaat
     2101 gtaaggagat ggcgtgcatc gggcaaactc cgtacagaga ggagaaaatt cgcgcaatca
     2161 tggcaccttt cgctaaggcg tgcaagccag gactttgcga ccagacaagc catcaacaag
     2221 gcaaaaagtt cataataaga aaatcaaaca atacagctaa caggaactct tgtggtcatc
     2281 ggccctttta tcaacgctag tgcagtctta ctgggtggcg ttctcggcgc actgctcagc
     2341 caacgcttac cggaacgtat ccgcgtctcc atgacatcta tttttggtct ggcatcgctg
     2401 gggattggta ttttactggt tgtgaaatgc gccaaccttc cagcgatggt tttagctacg
     2461 ctacttggcg ctctaattgg cgaaatttgt ttgctggaga aaggtgtcaa tacagcagtc
     2521 gccaaagcac aaaatctgtt tcgtcactca cgtaagaagc cagcgcatga atcttttatt
     2581 cagaattatg tcgcgattat tgtcctgttt tgcgccagcg gcaccgggat cttcggggcg
     2641 atgaacgaag ggatgaccgg cgatccgagt attttaatcg ccaagtcatt tcttgatttc
     2701 tttacggcga tgatcttcgc ctgctcgctg ggtattgcgg tgtcggtaat tagtatccca
     2761 ttactgatca tccagctaac gctagcgtgg gctgccgcgc tgatattacc gctgaccaca
     2821 ccgtcgatga tggcagactt cagcgccgta ggcggtttat tgctgctggc aaccggatta
     2881 cgcatctgtg gcattaaaat gttcccggtg gtcaacatgc ttccagcact ccttctggca
     2941 atgccgcttt ccgccgcctg gaccgcctgg tttgcctgac aatgcgtgca atagcggcaa
     3001 agtgatgata gattgtgcag tctgcagtaa attgaagaaa tttgattgac gagacgaggc
     3061 gaatcaggtt taatgcgccc cgttgcccgg atagctcagt cggtagagca ggggattgaa
     3121 aatccccgtg tccttggttc gattccgagt ccgggcacca aattcatatc aacggacctc
     3181 cacggaggtc cgtttttcgt ttcagaacgc cacgatttaa gcgttctgcc tacaaatcaa
     3241 ttctaccgaa ctcaaccaga ttccccccac atcaacccca ttgtgtgggt ataattgcgg
     3301 gcatacccca gttcgacaga atttgtaccc tctttaacgc attcagaagg ctgaactatg
     3361 gcactgactg acgcaaaaat ccgggctgca aagcccactg acaaggctta taaactcact
     3421 gacggggctg gcatgttcct gctggtacat cctaatggct cccgttactg gcgtctccgt
     3481 tatcgtattc tgggtaagga gaagactctg gcacttggtg tgtatccaga agtttctctc
     3541 tccgaagctc gtacaaaacg ggatgaggcc cgaaaactga tttcggaggg ggttgaccct
     3601 tgcgaacaga aaagagctaa aaaagtagtc cctgatttac agctctcttt tgaacatatt
     3661 gcgcgacgct ggcatgccag taataaacaa tgggcacaat cacacagcaa taaagtactc
     3721 aaaagccttg agactcacgt tttccccttt atcggcaacc gggatatcac aacactcaat
     3781 accccggatc tgcttatccc tgttcgtgct gcagaagcta aacaaattta tgaaatcgcc
     3841 agtcgtctgc agcaaagaat atctgccgta atgcgttatg ccgtacagtc tggcatcatc
     3901 agatataatc ctgctctgga tatggctggc gcattgacta cggtaaaacg ccagcatcgc
     3961 cccgctcttg atctttctcg cctgcctgaa cttttgtcgc gtattggcag ttacaaaggg
     4021 caacctgtca cccagcttgc cgttatgctg aatttactgg tttttattcg ttccagtgaa
     4081 ctcagatacg cccgctggtc tgaaattgat attgacaatg ccatgtggac tattccagcc
     4141 gaacgcgaac ccctgccagg cgtaaaattc tcacaccggg gctccaagat gcgaacacca
     4201 catcttgtgc cactcagcaa acaggctgta gccatactga cagaactaca gacatgggct
     4261 ggtgaaaatg gtctgatctt tacgggagca catgacccgc gtaaaccaat ca
//
LOCUS       DAZRUF010000281         4243 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZRUF010000281 DAZRUF010000000
VERSION     DAZRUF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.284"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..331
                     /locus_tag="ACHXOL_003853"
     CDS             <1..331
                     /locus_tag="ACHXOL_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR6810705.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKLSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
     gene            complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHXOL_003854"
     CDS             complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHXOL_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR6810706.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHXOL_003855"
     CDS             complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHXOL_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR6810707.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHXOL_003856"
     CDS             complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHXOL_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR6810708.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHXOL_003857"
     CDS             complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHXOL_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR6810709.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4035..>4243)
                     /locus_tag="ACHXOL_003858"
     CDS             complement(4035..>4243)
                     /locus_tag="ACHXOL_003858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810710.1"
                     /translation="FFCPFFAPSTAFCPIFAINNFPHKVISKPNSCSTFFLNIMSPAG
                     NNLIPVLGLKAVNDNHFHFVWVML"
BASE COUNT         1150 a         1041 c         1059 g          993 t
ORIGIN      
        1 aattgaagca tggcgcacag aacaggaggc gaagccgttc acgtttgaat ggaacggtcg
       61 tatctggaat gctggcccca actcactggg ccgcctttat ccggtggtaa tggctgcgaa
      121 atccgacatt gtacgggacg tgatgacgtg gggtgatgcc gataatcagc aggtgaaact
      181 gtcgatgccg gaactggaag aactggcggc agcaatggct caggcacagg ttgagcgcaa
      241 tgacgagatt tatcgccgtc agcgggagct gaaggaagag ctgaatagtc tgaaggattt
      301 gaattcggtt aggaatttta tcgtggaata acagaagctg cggcacgtcg tatgcaggaa
      361 cgtgccgcgg ttggctggta aactttcgat agtgcgagta ttgaatgatt tctaggcgtt
      421 atcgatttta cgtatttttt gcatgataga attcgtacct cctcccaacg accttccatg
      481 gctttccgga cttctgtagg ctggcggacg atgctgtcct cggatgttgt gctaattggg
      541 tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt gcgttgatgc tggtaacggt
      601 aaacgtcatg atgaacacga ccgcgaataa gacgacaatc gtcggagctg gtgcgctgtc
      661 gataaaaaag gacagccaga caccagtcat tgacactatt accgatatgc cagtcgccag
      721 taggagcgca atatgaaagc gttgtgtcag gagtactgca atggcgccag gcgcaatgag
      781 cagagaaata gagagaatga tacctactgc tttcagcgtc gccacaatgg tcagggaaac
      841 catgcacagc agcccataat gcagccatct cgtatgcaaa ccgcttacct gcgcctgttg
      901 ataatcaaag ctgaacagca aaaaatcccg ccattttgcg ctaataacaa gtgtaaccag
      961 cccggcaata atcaccgtct ggattatatc gccgatggtt atcccgagca tgtcgccgaa
     1021 aagaatatgg tcaagatgta cgtctggttt gattgcaata tacaagatca gacctgcggc
     1081 aaacatgcca gaaaaaacaa tccccataac tgtatcttgc ttgattcggc tgttgtcttt
     1141 caggtatccc gtcgccaccg cgcagaatac tccggcgacg aatgcccctg tagccagagg
     1201 taaccccaga atccaggtaa gcacgatgcc aggaaaaacc gcatgactca tcgcgtcacc
     1261 catcagtgcc caacctttca ggaccagaaa gaccgacagc agcgcacagg gtatggaaac
     1321 taccagtgaa atcagcagtg cgtgactcat aaaggtgaac tgcagtggct ccagtaataa
     1381 tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc gacgatttgc caaaagtcca
     1441 tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg tctgcgccac cacgatgatt
     1501 ccacctgtcg cgccgtccag aaagtaactg acccacgcgc cgaggaaact ggttacactg
     1561 ccaatagtta cagcaattat gagcaggcgg ggaaaacgat cggttaacaa ccatgctgtt
     1621 gcaccgggag tcaccaccag acagatcacc agaaaggcac caaccgtttg cagcgccgca
     1681 acggtcgaaa ccgacagcag ggtgaagaag aggattttta atctgcccgg atgtaatcca
     1741 atagcacggg cgtgattctc gtcgaaaaag gtcaccataa gatctttcca tttgaaaaaa
     1801 agcacgatga tcgagagaat gccaatgata gtgagttgca ggatatccgc cggatcgatt
     1861 gccagaatat tgcccaggac gatggtctga atgttcacgg atgtcgggtt caatgacacc
     1921 ataaacagcc ccaggccaaa aaaggacgag aagatcaggc cgatgatggc atcttctttt
     1981 aagcgggtgc gttggttaag aaacagcatg ctgcccgctg ccagtccgcc ggaaaagaag
     2041 gctcccagag aaaacggaag ccccagcata tatgcccccg caacacccgg gacaatagag
     2101 tgcgacagcg catcgccaat cagcgaccag ccttttagca tcagatagca tgacaggaaa
     2161 gcgcagagac cgccgaccat tgccgagacc cacatcgcat tgagcatata ttcatagctg
     2221 aagggttcca gtagcacgtt catcttgctt ccctctgcac cgctgacggt cgatgtgcca
     2281 caaaagggcg ttcatcgtca gtaataatgc tttcttccga gtcgttaagt gtgacatggc
     2341 gcagtacgcc gctaaaagcc agctccaggt tctcggctgt aaaagtggtg tctgtcggcc
     2401 cgcttgccag gacggtgcct ttgaccatga ccgtataatc gcaaaatgtc gtgactgagc
     2461 cgagattgtg cgttgagaca agcatcgttt tgccttccgc gcgtaactcc ctcaacaggc
     2521 tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa aggttcatcc agcaggatca
     2581 catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg tttcttttgt ccaccggaaa
     2641 gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac acgttccagc gcatcggtga
     2701 caatctgacg atccctcttt ttggctatac gcaacatacc catatgccca tagcggccca
     2761 tcatcaccac atcttccacc agtacaggaa atgaccagtc aacctcttct gactgaggga
     2821 cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg aatacccaga acagatatct
     2881 ttccgctggt cagacgcaca aatcccataa tcgctttaaa caacgtcgat tttccggaac
     2941 cgtttacccc aaccagagcg gcaatagatc cacttggtac tgtgaaggat gcatcgcgca
     3001 gtgctgtgtg cccgttacgc caggtgacag taacatcatt aacgacaatg cctgcagatt
     3061 gcatcattat ttctcccttt tcccggcttt aattccctgt accagcgtac tggtagtaac
     3121 cttaagaagg tcgatatacg taggtaccgg gccgttttct gtgctcaggg aatcgacata
     3181 gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc tgacgcgctg gtttatcgga
     3241 aatcgtactc tcgctaaaga ctgccgggat atgatttttc ttaactatat caacaacctt
     3301 acgtacctgc tgcggtgttc cttgttgatc ggcattaatc ggccacagat aaagctcttt
     3361 tagccccaaa tcgcgtgcga gataagaaaa agccccttca ctggtgacca tccatcgctg
     3421 attctcaggg agttccgtaa tctgcttacg caggggggca agggtttggg taatcttggc
     3481 tttataagta tcggcattac gttggtaggt ttgtgcattt gccggatcgt attttatcaa
     3541 cgcatcacga atattatcga cgtaaatcag agcattatct ggcgacatcc atgcatgggg
     3601 attaggtttg ccctcatagg gtccttcggt gatccctact ggcgtcacac ccgaagagac
     3661 aattacttct ggaaccccat tgagatgctg gtaaaagcgt tggaaccaca attccagatt
     3721 cataccattg gcgagaatca gttgtgcccc ctgcgcacgt ttaatatcgc caggggtagg
     3781 ctgatactca tgaatttctg caccaggctt ggttatggat gagacttctg cagcatctcc
     3841 agccacgttt ttggccatat ccgcgatgat ggtaaatgtt gtaatgacct tgaatttttc
     3901 agttgcactt gcctgaaatg cgatcgagca ggtgagggcg aggcacccca agagcatggt
     3961 tacttttttt attgagtgca taatttagta cctatagtga gcaaaattag aacaaacata
     4021 gcaacggcta tgttttatag cataacccat acaaaatgaa aatgattatc attaacagct
     4081 ttgaggccta aaacagggat taggttgttt cctgctggcg acatgatatt gaggaaaaaa
     4141 gtagagcagc tattaggctt tgaaatgact ttatgaggaa aattgttgat ggcaaaaatg
     4201 gggcaaaacg ctgtagaagg ggcaaaaaag gggcaaaaaa aga
//
LOCUS       DAZRUF010000282         4198 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZRUF010000282 DAZRUF010000000
VERSION     DAZRUF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.285"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(251..1570)
                     /locus_tag="ACHXOL_003859"
     CDS             complement(251..1570)
                     /locus_tag="ACHXOL_003859"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR6810711.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1701..3236)
                     /gene="gadC"
                     /locus_tag="ACHXOL_003860"
     CDS             complement(1701..3236)
                     /gene="gadC"
                     /locus_tag="ACHXOL_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR6810712.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(3392..>4198)
                     /locus_tag="ACHXOL_003861"
     CDS             complement(3392..>4198)
                     /locus_tag="ACHXOL_003861"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR6810713.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT         1077 a         1042 c         1065 g         1014 t
ORIGIN      
        1 gacagtttca atctacgcta ctgttcttca gaagagtata gcccatcgta attatttttc
       61 ggtgacagcg aatatcgtat ggtttttcat attcatacat ttttattagg gatttatagc
      121 tgtttaacta agtgtggtta attgcgtggt taatttgact tatgtaagca tgattactag
      181 tgggatattt cacaaggtgt aacaagccgg tgggtaaagc accggcttgt tacaaagtaa
      241 gcatgggagt ttaactgccc caacgacttt gcagatagct gaccgcttgt tgagtctgcg
      301 gtttattcag atagtcctca cggaacaaga tggtgccgct aatttctggt accgcatcgt
      361 taagatcgag ctgctttttc agttccggta cgccgccgtt aatcatccag tctggctcta
      421 tctttgaagg ttcacccact ttatagaagg cgataccgat atacaggcgg gtcctggtcg
      481 gtttaacgac atccgcccac cattttgcca acacgtcata acgcgcggca ctccgtgaga
      541 acggccagta aatttgggga gcaatgtaat ccagcaatcc ttgttccacc catcgacggg
      601 tgtcagcgta ggattcgtca taggctgccg cgcctcgggt atcggaaccg agcggatcgt
      661 gtgatcggtt acgccacacg cctgccgggc taacgccaaa ttcgactccc ggcttaatgc
      721 ttttaatggt gtgcgatact tttgcaatta actgctgagt attgttgcgc cgccagtctg
      781 cttttgacgc aaatgcgcct ccgtatttgc ggtacgtttc gttatcattt agccgtgaac
      841 ccggtgactc agtatagaaa tagtcgtcaa actgcacgcc atctaccgga tagcgggaaa
      901 ccacttctgc gactattgat gtgatccagt cctgaacctc agggatgccc gggtcgagga
      961 caaagcgatc gccagacgtt ctgatccagt cacggtgttg cacatagacg ctcgccggtt
     1021 gttgagacag agtgctattc agttccctga tagtaccggg cttcgtatta accgatacgc
     1081 gataggggtt aaaccaggcg tgtactttca tcccacgctt gtgggcttcg tcgagcataa
     1141 attgcagcgg atcgtaaccc ggattttcac caatcttacc ggtcataaga tcggaccatg
     1201 gcaaaatttt cgatggccac agggcggtac cgtccggctt aacctggaaa aagaccgtgt
     1261 ttataccgag acgttgcaga tgatccagtt tgtcgatcat cgcctgttgt tgtacacggg
     1321 cccggctggt ggggttacta atgttaaccg aggaaaccgg tggccagtcg aggcgagaca
     1381 ccgtggccag ccagatgcca cgcatcggtt gtgacgattg ttgcgtcgtg gctggtggct
     1441 ttgaacctgc tggtggtgtc accatggact ctggaggcgt gcttttacaa ctacacagta
     1501 aaagtgccag tgcaaccagt atcgctggtc ttctaatcgc taatttctcg tttcgggagc
     1561 agatatccat atatgctggt ttccggtaaa cagatgtgcg ctcattctcg tacttatccc
     1621 ggttaagtca atacgacagc gagcacgaaa aagggagcga tgaattatcg ctcccttgtc
     1681 ttataaccat tcagacatgg ttagtgtttc ttgtcattca tcacaatata gtgtggtgaa
     1741 cgtgcacgcg ggtgcaggaa gaagtgacct tttggtgcgt tctgactgtt gattggctcc
     1801 agagtgacgc cggtattcgc tttgccttta cgatcatgaa cagcatagag aataaagggc
     1861 agggcaagta ccaccaggaa actaacgacc agtaattcaa catacatatc ggtagagtca
     1921 ccctggatgt tatccggcgg caggaaggaa acaataaacg ccataattga agtcagcaga
     1981 ccgacaatag ccacgaccag tttcacccct ttaccaccag ggatattaaa tgtgcgtttt
     2041 aagtcaggat gtttaagaac caacacaatg tagccaataa acagcatgaa ataagcacac
     2101 agataaatca ccaccgtcag cgccagtgcg atcaggaagg acatgttgtt accgccaccg
     2161 gtattggtga ggatgatcaa cgcgatagaa gtaatcacca gctgtgaaat gaccagcgtt
     2221 accggtacgc catttttgtt cattttagcg aatgccgctg gcagcaggtt tttctgcgct
     2281 gtgacataca tcccgcgaga aggaccaaca atccaggagg cgatttccgc cagaacaccc
     2341 agcaacagca gtgcggagat cacgcgaacc gtccactcaa tttccggtgc cacatgggac
     2401 atcagaacgg taaaggtttg cattacccct gcggagaggt tgatttcatt acccggaatg
     2461 accatcgcaa tagacaaacc accaacagag cttaagcaga ttgccgctac catcagcagt
     2521 aacatagcca acggatagtc gcgccctggg ttgctcattt cattgacgtg ggttgcggat
     2581 gcttctacgc ccatataact caaaatgaag gcaacaaata caaccagggt gcccacttta
     2641 gagaagtcag ggaagaaggt ctttgaatcc atttcgatag caacgggggc accggagtgc
     2701 agataaatag ccgctaatgc gatcaaaata aatgcaggta acaggatacc ggcgaagaag
     2761 ccaactttag caattcgtgc cgtgtatttc gtgccaccaa actgcgttaa tgccagcgcc
     2821 caaagaatga tgagtgctgc aatagttttg gtaatggggt cttcattcag cgctggccat
     2881 ttcaggatgt aggagagtgc ccctaacacg aaatagagca tcggaataaa accaatggcg
     2941 atttgcagat agccaaatga gatcgctgca aatccccatc tcggccccag agtatttgat
     3001 acccaggcga agacaccacc ttcttcccag ccgtcgacgg tggccatttc cgcagcacaa
     3061 agtcccacgg gaataaacca taaaatcccg cctaatagca ggaagaagac taatgaaaag
     3121 cccgatgttg cgaaggtagg gtattcataa acagccatta ccatcgatgc cgttatggca
     3181 aaaaagccga gtaatgtgag ctgcttagct ttacctgtct gtactgatgt agccatatta
     3241 tccccctaaa acggtattcc tgtcggaacc gcacctgttt tgttctgaag cgtattcaga
     3301 acaatatttt ccgttgctaa tgccagtgaa cagactttgg aaattatccc gaaacgggtt
     3361 cgtttcggga caccgttacc gttaaacgtt atcaggtatg tttaaagctg ttctgctggg
     3421 caataccctg cagtttcggg tgatcgctga gatatttcag ggaggctttg tagtcttcca
     3481 gcaacagttc agcaaagtcc atttcgaagc cgcgacgaca cataatgcgc atcaccacga
     3541 tgtcggtggc ttcaccgccg agagtgaagg ccggaacctg ccagccgcgc agacgcagac
     3601 gttcagagag gtcgtacagg gtgtatcccg gatcttcacc ctctttcagt ttgaagcaaa
     3661 ccgccgggat gccttcgtcc gggcgaccgg cacagatgaa ctcatacggc cccagtttgg
     3721 cgatctcatc cgccagatag gcagcgacct ggtaggaagc attctgtact ttggtatagc
     3781 cttcacgacc aaggcgcagg aattcatagt actgtgcaat cacctgaccc gccgggcggg
     3841 agaagttgat ggcaaaagta ccaatctgac caccgaggta gtcaacgttg aacaccagtt
     3901 cctgcggcag cgcttcttcg tcacgccaga taacccagcc gcagcccagc ggagccagac
     3961 cgaatttgtg gcctgaagca ctgatcgatt tcacacgcgg caggcggaag tcccagacga
     4021 tatccggggc gacgaacggt gccaggaagc caccgctggc ggcgtcgatg tgcatgtcga
     4081 tgtcgatacc ggtgtcggcc tggaatttat ccagtgcatc gtgcagcggt tgcgggaact
     4141 catagttacc ggtgtaggtc acgccgaaag tcggcaccac gccgatggta ttttcgtc
//
LOCUS       DAZRUF010000283         4130 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZRUF010000283 DAZRUF010000000
VERSION     DAZRUF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.286"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            333..1673
                     /gene="dcuB"
                     /locus_tag="ACHXOL_003862"
     CDS             333..1673
                     /gene="dcuB"
                     /locus_tag="ACHXOL_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR6810714.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1751..3397
                     /gene="fumB"
                     /locus_tag="ACHXOL_003863"
     CDS             1751..3397
                     /gene="fumB"
                     /locus_tag="ACHXOL_003863"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR6810715.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3520..>4130
                     /locus_tag="ACHXOL_003864"
     CDS             3520..>4130
                     /locus_tag="ACHXOL_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR6810716.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
BASE COUNT          977 a         1104 c         1065 g          984 t
ORIGIN      
        1 agattaatct ataaacctaa tgacatctgc cctgagaaca aaaaatagac cgataaatat
       61 caataagata acagcaaaca aaacattaac atctgcgcag tacaaactat aaacccatcg
      121 ccagagagtc tttctctctg aaaaagccgc ttatcacagt gcataaattt gccgctgctt
      181 taatcagcca atattcactg tgaggtattt gctaaagccg gtaacgacca aacggatatt
      241 tagtcaggct ctgaaaacag ttcatacaaa acagaacgtg actgtgatct attcagcaaa
      301 aatttaaata ggattatcgc gagggttcac acatgttatt tactatccaa cttatcataa
      361 tactgatatg tctgttttat ggtgccagaa agggtggtat cgcgctgggt ttattaggcg
      421 gtatcggtct ggtcattctg gtcttcgtct tccaccttca gccaggtaaa ccaccggttg
      481 atgtcatgct ggttatcatt gcggtggtgg cggcatcggc gaccttgcaa gcttcgggcg
      541 gtcttgatgt catgctgcaa attgccgaga agctgctgcg ccgcaacccg aaatatgtct
      601 caattgtcgc gccgtttgtg acctgtacgc tgaccattct ttgcggtacg ggtcatgtgg
      661 tttacaccat tctgccgatc atctacgacg tcgccattaa gaacaacatc cgtccggaac
      721 gtccgatggc ggcaagttct atcggtgcac agatggggat catcgccagt ccggtgtcgg
      781 ttgcggtcgt gtctctggtt gcaatgctgg gtaatgtcac ctttgatggt cgccatcttg
      841 agttcctcga cctgctggca atcaccattc catcgacgtt aatcggtatc ctggcgatcg
      901 gtatcttcag ctggttccgt ggtaaagatc tggataaaga cgaagagttc cagaaattca
      961 tctccgtacc ggaaaaccgt gagtatgttt acggtgatac cgcgacgctg ctggacaaaa
     1021 aactgccgaa aagcaactgg ctggcaatgt ggattttcct cggggcaatc gctgtagtcg
     1081 cccttcttgg tgctgattcg gacctgcgtc catccttcgg cggcaaaccg ctgtcgatgg
     1141 tactggttat tcagatgttt atgctgctga ccggggcgct gattattatc ctgaccaaaa
     1201 ccaatcccgc gtctatctca aaaaacgaag tcttccgttc cggtatgatc gccatcgtgg
     1261 cggtgtacgg tatcgcatgg atggcagaaa ccatgttcgg tgcgcatatg tctgaaattc
     1321 agggcgtact gggtgaaatg gtgaaagagt atccgtgggc ctatgccatt gttctgctgc
     1381 tggtttccaa gtttgtaaac tctcaggctg cggcgctggc ggcgattgtt ccggtcgcgc
     1441 tggcgatcgg cgttgatccg gcatacatcg tggcttcagc accggcttgc tacggttatt
     1501 acatcctgcc gacttatccg agcgatctgg cagcgattca gtttgaccgt tccggcacca
     1561 cccacatcgg tcgcttcgtc atcaaccaca gctttattct gccggggttg attggtgtga
     1621 gcgtatcgtg cgtcttcggc tggatcttcg ccgcgatgta cgggttctta taaatgcact
     1681 ttgcgtgccg cccgtgacta cgcggcacgc cattttcgaa taacaaatac agagttacag
     1741 gctggaagct atgtcaaaca aaccctttat ctaccaggca cctttcccga tggggaaaga
     1801 caataccgaa tactatctac tcacttccga ttacgttagc gttgccgact tcgacggcga
     1861 aaccatcctg aaagtggaac cagaagccct gaccctgctg gcgcagcaag cctttcacga
     1921 cgcttctttc atgctccgtc cggcacacca gaaacaggtt gcggctattc ttcacgatcc
     1981 agaagccagc gaaaacgaca agtacgtggc actgcaattc ttaagaaact ccgaaatcgc
     2041 cgccaaaggc gtgctgccga cctgccagga taccggcacc gcgatcatcg tcggtaaaaa
     2101 aggccagcgc gtgtggaccg gtggcggtga tgaagaagcg ctgtcgaaag gcgtctataa
     2161 cacctatatc gaagataacc tgcgctactc gcaaaacgcg gcgctggaca tgtacaaaga
     2221 ggtcaacacc ggcactaacc tgcctgcgca aatcgacctg tacgcggtag atggcgatga
     2281 gtacaaattc ctttgcgttg cgaaaggcgg cggctctgcc aacaaaacgt atctctacca
     2341 ggaaaccaaa gccctgctga ctcccggcaa actgaaaaac ttcctcgtcg agaaaatgcg
     2401 taccctcggt actgcagcct gcccgccgta ccatatcgcg tttgtgattg gcggtacgtc
     2461 tgcggaaacc aacctgaaaa ccgtcaaatt agcaagcgct cactattacg atgaactgcc
     2521 gacggaaggg aacgaacatg gtcaggcgtt ccgcgatgtc cagctggaac aggaactgct
     2581 ggaagaggcc cagaaactcg gtcttggcgc gcagtttggc ggtaaatact tcgcgcacga
     2641 cattcgcgtt atccgtctgc cacgtcacgg cgcatcctgc ccggtaggca tgggcgtctc
     2701 ctgctccgct gaccgtaaca ttaaagcgaa aatcaaccgc gaaggtatct ggatcgaaaa
     2761 actggaacac aatccaggtc agtacattcc acaagaactg cgtcaggccg gtgaaggcga
     2821 agcggtgaag gtcgacctta accgcccgat gaaagagatc ctcgcccagc tttcgcaata
     2881 tccggtttcc actcgtttgt cgctcactgg caccattatt gtgggtcgag atattgcaca
     2941 cgccaagctg aaagagctga ttgacgccgg taaagaactt ccgcagtaca tcaaagatca
     3001 cccgatctac tacgcgggtc cggcgaaaac ccctgccggt tatccatcag gttcacttgg
     3061 cccaaccacc gcaggtcgta tggactccta tgtggatctg ctgcaatccc acggcggcag
     3121 catgatcatg ctggcgaaag gcaaccgcag ccagcaggtt accgacgcgt gtcataaaca
     3181 cggcggcttc tacctcggta gcatcggcgg tccggcggcg gtactggcgc agcagagcat
     3241 caagcatctg gaatgcgtcg cttatccgga gctgggtatg gaagctatct ggaaaatcga
     3301 agtagaagat ttcccggcgt ttatcctggt cgatgacaaa ggtaacgact tcttccagca
     3361 aatcgtcaac aaacagtgcg cgaactgcac taagtaacct cttcggccca gcgcctggca
     3421 gcatgctgcc aggtgatccc cctgggccac ctcttttgcg attgtaattt catgcttgct
     3481 ggtgaatagt cagtattttc cctgacttgc gaactcacca tgacccgcac actcaaaccg
     3541 ttaattctta acaccggcgc actggcgcta acgttaatcc tgatttatac cggcatttcg
     3601 gcccatgaca aactcacctg gctgatggaa gtgacaccgg tgattattgt cgtgccgcta
     3661 ctgcttgcca ccgctaaacg ttatccgtta acgccgctgc tctatacgct cattttcttt
     3721 cacgccatca tcctgatggt cggcgggcaa tacacctacg cgaaagtccc catcggtttt
     3781 gaggtgcagg aatggctggg gttgagccgt aatccgtatg acaagctggg gcattttttc
     3841 caggggctgg tgcctgcact ggtggcacga gaaattctcg tgcgcgggat gtacgtgcgc
     3901 ggacgtaaaa tggtggcgtt cctagtctgc tgcgtggcgc tggcgataag cgcgatgtat
     3961 gaattaatcg agtggtgggc ggcgctggca atgggccagg gagcggatga ttttctcggc
     4021 acccagggcg accagtggga cacgcaatcc gatatgttct gcgcgctgct tggcgcatta
     4081 acgacggtga tattcctcgc tcgctttcat tgccgccagc tacggcgctt
//
LOCUS       DAZRUF010000284         4087 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZRUF010000284 DAZRUF010000000
VERSION     DAZRUF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.287"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..877
                     /gene="adeQ"
                     /locus_tag="ACHXOL_003865"
                     /gene_synonym="yicO"
     CDS             <1..877
                     /gene="adeQ"
                     /locus_tag="ACHXOL_003865"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR6810717.1"
                     /translation="GLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIIT
                     VLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELA
                     GIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSS
                     VTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMT
                     SSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCV
                     VVVASLFALKIILVD"
     gene            930..1382
                     /locus_tag="ACHXOL_003866"
     CDS             930..1382
                     /locus_tag="ACHXOL_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR6810718.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1593..2773
                     /gene="nepI"
                     /locus_tag="ACHXOL_003867"
                     /pseudo
     CDS             1593..2773
                     /gene="nepI"
                     /locus_tag="ACHXOL_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2814..3107)
                     /gene="yicS"
                     /locus_tag="ACHXOL_003868"
     CDS             complement(2814..3107)
                     /gene="yicS"
                     /locus_tag="ACHXOL_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR6810719.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3329..>4087
                     /gene="nlpA"
                     /locus_tag="ACHXOL_003869"
     CDS             3329..>4087
                     /gene="nlpA"
                     /locus_tag="ACHXOL_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFR6810720.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFLHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
BASE COUNT          899 a          906 c         1077 g         1205 t
ORIGIN      
        1 tggattattt atcgccttaa tgggattaaa aaatactggc gttattgtcg ccaataaaga
       61 cacgctggtg atgattggcg atttaagttc tcacggcgtg ttgttaggta ttttagggtt
      121 ttttattata accgtgttgt catcacgtca ttttcatgcc gcggtgctgg tttctattgt
      181 ggtgacgtct tgctgtggat tatttttcgg tgatgttcat tttagcggcg tctattccat
      241 tccgcctgat attagcggcg tcattggtga agtagatttg agcggcgcgt taacacttga
      301 actcgccggt atcattttct cctttatgct gatcaaccta tttgattcat caggaacatt
      361 aattggtgta actgataaag cgggcttaat agatggtaac ggtaaattcc ccaatatgaa
      421 taaggcgctg tatgttgata gcgtcagttc ggtggcgggt gcgtttatcg gcacctcgtc
      481 tgttactgcc tatattgaaa gtacttctgg tgtggcagtc ggtggccgca cggggctgac
      541 tgcggttgtg gttggcgtta tgttcctgtt ggttatgttc ttctccccgc tggtggcgat
      601 ggttcctcct tacgcaaccg ccggagcgtt aatctttgtt ggcgtgctga tgacttcgag
      661 cctggcgcgc gttaactggg atgattttac cgaatcggtg cctgcgttta ttaccacggt
      721 gatgatgccc tttactttct cgatcaccga agggattgca ctcggcttta tgtcgtactg
      781 catcatgaaa gtgtgcaccg ggcgctggcg cgatctgaac ctgtgtgtgg tggtggtcgc
      841 atctctgttt gcactgaaga ttattctggt ggattagtaa ctggcgaggg tttccagata
      901 tcatgagttc tgataacgca ggagaactca tgatctggat aatgctcgcc acgctggcgg
      961 tagtgtttgt ggttggtttt cgggtgctga catccggggc cagaaaagcg attcgccgtc
     1021 tcagtgatcg gctgaatatc gatgtcgtac ccgtggagtc gatggtcgat caaatgggaa
     1081 agtcagccgg tgacgaattt ttacgttatt tgcatcgccc ggatgagtcg cacctgcaaa
     1141 acgccgcgca ggtgttgctc atctggcaaa ttgtcattgt cgatggtagc gaacagaacc
     1201 tgctgcaatg gcatcggatt ttacaaaaag cccgcctttc cgcgccgatt accgatgctc
     1261 aggtcaggct ggcgctgggt tttctgcgcg aaaccgaacc tgaaatgcag gatattaatg
     1321 cttttcagat gcgctataac gcgttctttc agcctgccga gggcgttcac tggctgcatt
     1381 aaaaccgctt cgtcttatca gcctacagac cgcgcctgac ctgtcgggtg gataagacgt
     1441 tcacgccgca tccggtaatc tttgccctgt tccgtttcga tcttaactcc agctcaagca
     1501 acaccgtaaa agttgcaaaa acgttaaatg cgtcacacat ttcaatgtta aagtggtcgg
     1561 cttttcccct gaaacatgcc acgggtaaca ccatgagtga atttattgcc gaaaaccgcg
     1621 gcgcggatgc catcacccga ccgaactggt cagccgtttt ctcggtggcg ttttgtgtcg
     1681 cctgtctgat tatcgttgag tttttgcccg tcagtttgtt gacgccaatg gcccaggatt
     1741 taggcatttc ggaaggggtt gccgggcaat cggtgaccgt gaccgccttt gtggcaatgt
     1801 ttgccagttt gtttattacc cagacaattc aggctactga ccgccgcaac gttgttattt
     1861 tgtttgccgt tttgctgacg ctctcctgct tgctggtttc ctttgctaac tcattcagtt
     1921 tgcttttaat cggtcgtgcc tgtctggggc tggcgctggg cgggttctgg gcgatgtcgg
     1981 cgtcgctgac catgcgtctg gtgccgccgc gtacggtgcc gaaggcgctg tcggtgatct
     2041 tcggcgcggt ttctattgcg ctggtgattg ccgcgccgtt gggcagtttt ttaggcgagc
     2101 ttatcggttg gcgcaatgtc tttaatgcgg cggcggtgat gggcgtgctg tgtattttct
     2161 ggattatcaa atcattgcct tcactgccag gcgaaccctc gcatcagaaa caaaatactt
     2221 tccgcttatt acaacgtccg ggtgtgatgg cagggatgat cgccatcttc atgtctttcg
     2281 ccgggcagtt tgctttcttc acatatattc gcccggtgta tatgaacctg gcgggattca
     2341 gcgtggatgg cttaacgctg gtgctgttga gttttggcat tgccagcttt atcggtacgt
     2401 cgctttcgtc attcattctt aaacgttcgg taaaactggc cttagcaggc gcgccgttaa
     2461 tactggctgt gagtgcgttg gtgctgacgt tgtggggaag cgataaaatc gttgctaccg
     2521 gcgtggcgat tatctggggg ctaacttttg cattggttcc cgtcggctgg tcaacgtgga
     2581 tcacccgctc actggccgat caggcagaaa aagccgggtc tattcaggtg gcggttattc
     2641 agcttgtggc gcggcaatcg gcggttatgc gctggataat attggtctga cttcgccgtt
     2701 gatgttttct ggcacattga tgttgctgac tgcattgttg gttactgcaa aggtgaaaat
     2761 gaagaaatcc tgatatcagg taaagaagcg ttggtcagta gttgttgctg ttcttacata
     2821 tccgggcatt ctaccttacc caacgcttcc caataattgc tctgattatt acgttccatc
     2881 gccacgatgg cctcgcgtat atgttgttta ttgttttcat ctgacaacat taacttttcc
     2941 cacgcttcat ctgataatga cgcctgtggc gtgcaaattt tacgtaaatc ttgtaatacc
     3001 gtggttttct cttttgcgga ctgcagtgag ctaaaaggcg actcggcgta tacgatgcct
     3061 ggcatagcga aaaaagtgaa aataagcagt agtgtcgttg gtttcatgga ttcaaccttt
     3121 tacaaatgtg tatcgaatga agcataagtg tagtttgctt tcatcgctct catcatgctg
     3181 tacgaaacgc gcttgcgtgg tcagtaagaa gtgccagact ttatattcca cttttattcc
     3241 tttttattct tatcgatagc gtttcgtttt ttaaaccgca gcgaccttac cgctatagtc
     3301 aggtaatcat taataaaagg ataaaaaaat gaaactgaca acacatcatc tacggacagg
     3361 ggccgcatta ttgctggccg gaattctgct ggcaggttgc gaccagagta gcagcgatgc
     3421 aaaacacatt aaagttggcg ttataaatgg cgcagaacaa gatgtcgcgg aagtcgctaa
     3481 aaaggtggca aaagagaaat atggcctcga tgttgaactg gtaggattta gcggttcgtt
     3541 gctaccgaac gatgcgacaa atcatggcga acttgatgcg aatgtcttcc tgcatcgacc
     3601 ctttcttgaa caagataatc aggcgcatgg ttataaactg gtagcggtgg gaaatacgtt
     3661 tgtcttccct atggcgggtt attccaaaaa aatcaaaacc gtggcgcaaa taaaagaagg
     3721 cgcgacagtg gcgatcccca acgacccgac caaccttggt cgcgcacttt tactgttgca
     3781 aaaagagaag ctaattactc tgaaagaggg aaaagggtta ttacctaccg cgctggatat
     3841 aaccgataat cctcgtcatt tgcagattat ggaactcgag ggggcgcagc tcccgcgcgt
     3901 actggatgat cccaaagttg atgtagcgat tatcagcacc acttacattc agcagacagg
     3961 gctttctccg gtgcacgaca gcgtatttat tgaagataag aattcgccgt atgtgaatat
     4021 tttggtggca cgggaagata ataagaatgc agaaaacgtg aaggaatttc tgcaatctta
     4081 tcaatca
//
LOCUS       DAZRUF010000285         4075 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZRUF010000285 DAZRUF010000000
VERSION     DAZRUF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.288"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHXOL_003870"
     CDS             <1..784
                     /locus_tag="ACHXOL_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR6810721.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1756
                     /locus_tag="ACHXOL_003871"
     CDS             797..1756
                     /locus_tag="ACHXOL_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR6810722.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="ACHXOL_003872"
     CDS             complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="ACHXOL_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR6810723.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="ACHXOL_003873"
     CDS             complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="ACHXOL_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR6810724.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1224 a          952 c          861 g         1038 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaatagagc ttccaggaag gtcatcttat aatttagaag aaatgctatc
     1141 tgcgataaat ttcaccattc catactctat cgcatctgta tcagatagtt ccaacaccgc
     1201 agatattttt ccaggaactc cttttccttt gttgttcgaa attgaagtga gttcatgtac
     1261 agggaataat actaattgta atagcgtccg ctttaattat aatttaaata ctattttaca
     1321 ggtaaattta atgacctgtg gttttgaaga tcaatccatc gacactggac gatttgtact
     1381 gtctcgtatc aataatgaaa attatcagtt tcatcatata agatttgact gcatccaggg
     1441 cgagcaaggt gaattagttt ttgaacccag tgatgttgaa tattactttg aaccagtgac
     1501 gatacaggag tcaggaacca gcatattaaa aaatgagtta gaaaacagtg aggacggtgc
     1561 gggtcaagta ggctttcaac tctccaatga tggtacgaat gaaatccagt atggtaaaag
     1621 caattactac tcttttcagc atccccatga aggaagtaat cagattcctc tatttattcg
     1681 cccgcgtaca tatggaaata atgtctcaag cgggcaaata atgagccgag tcaaaatcgt
     1741 ggttatgtac aactaatcta tctcccccac cttaactttg ggggaggtgc cgattaacgc
     1801 gccgctggac taaaaagcgc agaataatcc gtattgcaga ttcgctgtac cgccgggtgc
     1861 tgaatcatcc gctcagcaaa aatagcatgg tactcttcca tcacattctc gacgcgacca
     1921 atttctacga cagttttatc ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag
     1981 attgcattgt gcatcgcacc aaaagctttc atcaacgcgg catcatcaaa ctcgccgagg
     2041 atttctacgt ttaatccctg ggagttaaac cagttaagca atttgcgccc taacattgaa
     2101 cgtcgcccag gaatcaaaag tcgccgttct tccagacaag ccgggaacga tttttctggt
     2161 ggtggatttg tacaccagaa actcacgcca cattcgccaa ttctcacgga gaacaggcct
     2221 tcctgctgcg tagagtctat cggacagtca gaaatgatca tatccagttt atgctgactt
     2281 aattgctcca gcagcatttc gtgggtggat tcgaagcagc gaagatgaat gggctcgcct
     2341 tctaccactg cggcgttaag tacgctactg accaggcgtt tggaaagtgc atcagccacg
     2401 ccaacgtcaa acaataaatt ggattctttg cgatagttca caatatccag catttcctgg
     2461 cttaaggtga acattttatc ggcatagcga tagaccagtt ctcccagctc gctgggttcg
     2521 agaccacgtc ccttgcgttt aaataatttg ccttgcaggc gctcttccag cgctcgaatc
     2581 tgtccggtaa tggtttgtgg agttaaataa agcgcctccg ctgcgccaac cacggaacct
     2641 tctttataga catgccagaa gtaatacaag tggttgtaat tgatatgaga catgctcatt
     2701 tctctccctg ataacaatga aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc
     2761 aaactgatgg acgcaaacga acgcgtaacc agctgtatcc aattaccgcc gaagagatag
     2821 aaccgaccag gataccgagt ttcgcccagt taatcagttc tggatctacg ctaccaaagg
     2881 ccaggctggc aataaagata gacatagtaa aaccgatacc gcacaggatc cccaccgcca
     2941 taatttgctg ataagtcgtt ccctcaggca gatgcgccag tttcaaacgc agcgccaacc
     3001 agcagaacag actaatcccc agcggtttgc caatcagcaa gccagcgatg atccccaatg
     3061 gcagaatgga ggtcaagcca tccagcgtga cgccttgcag tgaaacgcca gcattagcaa
     3121 atgcaaacag cggtaaaatc agatacgcca cccacgggtg caatacatgc tccagtcgct
     3181 tcgctggaga acgcccatgc ttctctttca aaggaataaa gaagccgaca attacccccg
     3241 ccagggttgc gtgaaccccc gatttcaaca ccgcagtcca caacaccacg ccaacaagaa
     3301 tatagacgcc cgtgcggcgt acaccacaca gattcaatac cgcgagtacc gcaattgcta
     3361 cagccgcgac gccaagagag gccatcgata agtcattagt gtagaacaat gcgatgataa
     3421 tgatggcccc aagatcgtcg ataatagcca gagccatcaa aaagatcttc agtgctaacg
     3481 gaacacgact tcccaacagc gccagcacac caagtgcaaa ggcaatgtca gtagccgccg
     3541 ggatcgccca cccttcgcgg gtaatcggat cggcatagtt aaaggccaga tagagtaatg
     3601 ccggaacaat catcccacca atagcggcga taaccggaaa tgcggcctgg cgtaagctgg
     3661 ctagcgatcc ttgcatcagt tcacgtttaa cttccagacc gactaacagg aaaaataccg
     3721 ccatcagcgc gtcatttatc cataacagca tgtttttgtt gatttcgagt gaaccaaccc
     3781 ggagctgaac cggcgtctcc agaaagtcgt gataccatcc actggttgcg ccgctgttgg
     3841 ccatcatcat cgccaggata gcggcaatga taagaataat gcctcccgag gcatcactgc
     3901 taaagaatcg atgcagatgt ttcacttttt atttctcttt caggtgaata gatcgttttt
     3961 actattttac cccgcacaaa tcatcgttaa aatcagatta tagtgtagga ataactcgtt
     4021 ttttacgagc aattaaagga tttgcgccat cagcggtgag atgagttttt gacgg
//
LOCUS       DAZRUF010000286         4050 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZRUF010000286 DAZRUF010000000
VERSION     DAZRUF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.289"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            32..760
                     /gene="mpaA"
                     /locus_tag="ACHXOL_003874"
     CDS             32..760
                     /gene="mpaA"
                     /locus_tag="ACHXOL_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR6810725.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(735..1700)
                     /gene="ycjG"
                     /locus_tag="ACHXOL_003875"
     CDS             complement(735..1700)
                     /gene="ycjG"
                     /locus_tag="ACHXOL_003875"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR6810726.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1819..2325
                     /gene="tpx"
                     /locus_tag="ACHXOL_003876"
     CDS             1819..2325
                     /gene="tpx"
                     /locus_tag="ACHXOL_003876"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR6810727.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2369..3910)
                     /gene="tyrR"
                     /locus_tag="ACHXOL_003877"
     CDS             complement(2369..3910)
                     /gene="tyrR"
                     /locus_tag="ACHXOL_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR6810728.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          970 a         1143 c          973 g          964 t
ORIGIN      
        1 gcctgtaata cttcctgaac cagagacgat catgaccgta acccgcccac gcgccgaacg
       61 cggcgcattt ccgcccggaa cagaacatta cggacgttca ttattgggcg cgccgttaat
      121 ctggtttcct gcccctgcag ccagccgcga aagtggtttg attctggctg gcactcacgg
      181 tgatgaaaac tcttcggtcg tcacgctctc ctgtgctctg cggacgttga caccttcttt
      241 gcgccgccat catgtggtgc tgtgtgtgaa tcctgacggc tgccagctgg ggttacgggc
      301 caatgctaat ggtgtggatt taaacagaaa ctttccggcg gcgaactgga aggaaggtga
      361 aacggtttat cgctggaaca gcgccgctga agaacgtgat gtcgtattac tgacaggtga
      421 caaacctggc tcagaacctg aaacccaggc gctgtgccag cttatacacc gcattcaacc
      481 tgcctgggtg gtctcattcc acgatccact ggcctgtatt gaagatccca gacacagcga
      541 attaggcgaa tggctggccc aggcgtttga attgcctctg gtaaccagcg tcggttatga
      601 aacgcctggc tctttcggca gttggtgtgc agatctcaac ttgcattgca tcaccgcaga
      661 gtttccgccc atctcctccg acgaagccag cgaaaaatat ctctttgcga tggcaaactt
      721 gctgcgctgg catcctaaag atgcaattcg cccgtcgtga actgaagcgc cggttccaca
      781 tctaccgcca gccaggtcgg tccgtcaaga tcggcgaaac tgacctgcgg caccagcggt
      841 aaagcggcgc taatggcacg agaggtacac aacatgcagc ccagcatcag actgaaacct
      901 tgtgcacgcg cttcagtcgc cagcgccagc gcttccgtca gaccaccgat tttatcgagc
      961 ttaatgttaa ccatctcata gcgccctttc agcgccttca gattgctacg tgtatgacaa
     1021 ctctcatcag cacaaatcgg caacggatga ataaaattct ccagcgccgc atcgtcctgc
     1081 gcaggaaggg gttgttcaag catcgcaacg cctaaatccg ccaatagctg gcaacgcgcc
     1141 gccaaccctt ctgcacgcca ggattcattt gcatcaacga tcagcgtcgc atcgggcaca
     1201 gctgtgcgaa ttgccaccat ccgctcacta ataagatggt tatccagctt cactttcagt
     1261 aatttcgcgc ctgcctgcca gagtgttgat gcactattgg ccatctgatc aggcgtaccg
     1321 atgactaccg tctgtgcagt gataactgtc tcgggaagcg ttatgccgat caaatcagcc
     1381 agcgattgct gctgttttcg cgcggccaga tcccacaatg cacaatccag cgcattacgt
     1441 gctgcgccgg cagggagaat tttttgcaac tcctcccgtg tcagcccttt ctctaattgc
     1501 gacacgacgc tcataatttg cgccattacc gaggcatcac tttctccata acgcggatac
     1561 ggcgtgcatt cgccggtgcc tttaataccc tcttcttcca gttcaaccac caccacgcgc
     1621 gcttcactgc gacttccccg ggcaatcaca aacggggtat gtaagggcca ggcttcctca
     1681 aataccttaa cggttctcat ctttactcct tgttgtcgcg gtggtctgcg gattaagcgc
     1741 aaatagcgtt tgctgtgtta ttgacagtta gcataaacta ggtgtgacgt taactatatg
     1801 taaacaggaa agataatcat gtcacaaacc gttcatttcc agggcaaccc ggttacagtc
     1861 gccaattcca tcccgcaggc gggtagcaaa gcgcagactt ttactctcgt ggcaaaagat
     1921 ctgtctgacg tcaccctcgg tcagtttgcg ggtaaacgca aagtgctgaa cattttcccg
     1981 agtattgata ccgatgtttg cgccgcatca gtacgcaagt ttaaccaact ggcaactgag
     2041 atcgacaaca ccgttgtgct gtgtatctct gccgatctgc cgttcgccca gtctcgtttc
     2101 tgcggcgcag aaggtctgaa caacgttatc accctctcca ctttccgtaa cgctgaattt
     2161 ctgcaagctt acggtgtggc aattgctgat ggcccactga aaggtctggc agcgcgtgcc
     2221 gttgtggtta ttgacgaaaa tgacaatgtg attttcagcc agctggtgga tgaaatcacc
     2281 accgagccgg attacgaagc agctctggct gtactgaaag cataagttaa tatgcctgat
     2341 ggtgttgcac catcaggcat attcgcgctt actcttcgtt cttcttctga ctcagaccat
     2401 attcccgcaa cttattggca atcgcggtat gtgaaacgcc gagacgtttt gccagtttgc
     2461 gcgtgctggg ataattgcga taaagctggg ttaataccga gcgttcaaaa cggctggtga
     2521 tttcgtccag cgaaccttcc atcgcatctt cgcccacggc taccgttgcg gcgtcataat
     2581 ccggcaacaa aatatcctgt ggacgcagct cataaccgtc cagttgtgtc agtgcgcgat
     2641 agatagcgtt ctttaactgc cgcacatttc ccggccacgc ataacgcgta agtacagtat
     2701 tcaggtcagc ggccagtttc ggacgctgca cgccctgctc gtcggcaaag cgggcgacga
     2761 acagctcagt taacggcatg atgtcctgcg gacagtcacg tagcggcggc agattgagcg
     2821 tcaacacgtt cagacgataa tagagatctt cacggaacat gcctttttgc accagttcga
     2881 ccagattctt ctgcgtaacg caaatcaccc gcacatcgac atgcacctca tggtcttcgc
     2941 caacccgacg gaaagtgcca tcattaagga aacgcagtaa tttcgcctgc atccgtggtg
     3001 acatttcccc tatttcatcc aacagcaccg aaccaccgtt cgcctgctca aagaatcctt
     3061 tcttcccttc cggagcatga ccaaacagtt cactctcgac cgcatcttcc ggtatagacg
     3121 cacagttcag cgccaggtaa ggtttgcccg ctctggggct tgcctgatgg caggcgtagg
     3181 caaagagatc tttacctgta cctgtgtcac ccgtaatcag cagcggcgcg cttagcatcg
     3241 ccagtttctg cgcctgttcg acaacatgct tcattttcgg gctgacggcg acaatttgac
     3301 tgaaggcgct gacgtcctgg gcggcgacat tttgcaactg gcggcccata cgaatcgttg
     3361 atcgcaacat caccaccgca ccggtcagga cgtgttgatc attttcatcc tgaagataaa
     3421 caggcgtaat ctccatcagg aaattctgcc cattaataac gacatgctcg ttatgcgaat
     3481 cttgcggttc gctttccagc caacgtaaaa aattaaagcc gttaatcaat tgtgcggcgg
     3541 tatggttgcg caggcgatcc aatttttgcc caaaaagctg acagctcgcc gggttcgcca
     3601 tatccacttt gcttttcata tcgacagaga gcacaggttc aggcaacgcc tccagtaacg
     3661 cgctcaacgc cagatgctca cgttcggaag gcatccacgg gacagtacgc acatcggtaa
     3721 cacccgcaat acggcgtatt tcggccatca gactgctgaa actctcaaac tccagttcag
     3781 caaaattgag gtagattcgc ccaatgggat caatctcaat accgcgtaaa tcaatgcctc
     3841 ttagcacgag tagatcgagt aattcgcggg tcagaccgag tcggtcttca caaaagactt
     3901 ccagacgcat gggaaccttc acctgaaaaa agaacaatta atatgatgat atgacactat
     3961 ttggatagca ggaaggtttc tgtcaacaat cattgacaca aagacggaaa aacgggcact
     4021 tcggcgtaaa gatatccggt cagcacccga
//
LOCUS       DAZRUF010000287         4043 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZRUF010000287 DAZRUF010000000
VERSION     DAZRUF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.290"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            376..483
                     /locus_tag="ACHXOL_003878"
     CDS             376..483
                     /locus_tag="ACHXOL_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR6810729.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(570..2249)
                     /gene="bcsG"
                     /locus_tag="ACHXOL_003879"
     CDS             complement(570..2249)
                     /gene="bcsG"
                     /locus_tag="ACHXOL_003879"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR6810730.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2246..2437)
                     /gene="bcsF"
                     /locus_tag="ACHXOL_003880"
     CDS             complement(2246..2437)
                     /gene="bcsF"
                     /locus_tag="ACHXOL_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR6810731.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2434..4005)
                     /gene="bcsE"
                     /locus_tag="ACHXOL_003881"
     CDS             complement(2434..4005)
                     /gene="bcsE"
                     /locus_tag="ACHXOL_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR6810732.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1033 a         1011 c         1053 g          946 t
ORIGIN      
        1 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg
       61 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct
      121 gcggaatgcg cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag
      181 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
      241 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
      301 tcacgggggc taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttggcattac
      361 aacacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
      421 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag
      481 taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
      541 gaggttaaac tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg
      601 ttcaggcgaa cgtacggttt atcctggtat tgaataacta ctgcatttga gttctcggag
      661 accggtgctg tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg
      721 aaaatcttgc catcaagaac gcgaaccacc agatcggaga tagctaggaa gctgctcggt
      781 tggtcaatga caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca
      841 acggggacgt ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg
      901 tcgcccttca gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat
      961 ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg
     1021 taatccgctg ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg
     1081 tagaacgtgg cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc
     1141 acggcggtat cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt
     1201 gtttgatcca tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg
     1261 ccgaactggc cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgaaaggtta
     1321 tcaaacagat agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg
     1381 cagctggcgc gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag
     1441 ttcttgaact caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct
     1501 atatccgacc aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca
     1561 gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc
     1621 agccaggcgt taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact
     1681 accgatgcgc cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca
     1741 gtggtcgtcg gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg
     1801 ttcagccata gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat
     1861 aaccaggcca ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg
     1921 acaaggtcga ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg
     1981 ctttccgggc caggcaacca ggtgtcatgc cagaacaaag caaagccgat cggcagggca
     2041 atccagtggc gcaagcgatg caggctgtag cgcggaatgg gcatcagcag aaacgtggca
     2101 aacaccaaat tgaggagcgg atggaagtta agatatcccg cccacaacag gccgaactta
     2161 accagaaaat agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc
     2221 atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc
     2281 gtaacgtccc ggccggttta acataacgag gtttagcaaa gaataaacgt aaggtgtcgc
     2341 gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa
     2401 cgacaataat ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc
     2461 taacagtcgc atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat
     2521 gaccggttta gaactttgcg ttaaaggcag cggcatgccc cattgttctg gggcaagcaa
     2581 gcgcatctgc accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga
     2641 gaaaatgtcg ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat
     2701 ccgacagaat gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt
     2761 acggttaggg cgacacagcg tcagggcttg ttcaacgcgg atacccggta ccggacgtag
     2821 ggcaaccaga acgcctttac cgtgggcagg taatagaggg ttattcatca tgttgttgac
     2881 ggcattacag aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga
     2941 cagcaaggta gtgatatctt ccggcacata gcgactaaac ttctgccctt gcacgctttc
     3001 gatcatcgtc agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca
     3061 ggccaataac aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat
     3121 tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat
     3181 ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt
     3241 gaacaggact tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc
     3301 cagtacagca acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc
     3361 ttcgctttga actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc
     3421 gctgacccct ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa
     3481 acgcaaactg gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt
     3541 atcgttatta tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt
     3601 attcatttta tccaaccatg agcgaagccg ctcggcagga atgttttgcc atgcgttatt
     3661 tgcgcaaaca agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc
     3721 caaatagtat agaccttttt catgatttag cattgaaaat aattttattt tttccggccc
     3781 ttgagaatca tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac
     3841 gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg
     3901 gcgatcgacg ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa
     3961 tgatgagata ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct
     4021 gattatcagt ttactagcga aag
//
LOCUS       DAZRUF010000288         4028 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZRUF010000288 DAZRUF010000000
VERSION     DAZRUF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.291"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..417
                     /locus_tag="ACHXOL_003882"
     CDS             <1..417
                     /locus_tag="ACHXOL_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810733.1"
                     /translation="DEINKKSMSRVKKHKERLMEITPEIFFRFLSEKGVSSVCPSCGS
                     SRLSVPESMDLCWDKNKKPENFNNLPLEEQSELIKENIKHYVSYAFLGDVKSIPDMRK
                     TYYTLHCLNCGYLSLYRTSAVLKWLEKDKAQDGDNG"
     gene            518..955
                     /locus_tag="ACHXOL_003883"
     CDS             518..955
                     /locus_tag="ACHXOL_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HFR6810734.1"
                     /translation="MSDKLERRIERLEGDLSLTRNDLATLAERTTNLSTKADVGEVKG
                     ELKADIAHLKGDLECDIANLKGELKSDTANLKEQLKSDINSLKGELTEAMDKRFDKIM
                     DEMNRRFDKVDDNTKWRWSGIIVPVCTTIFTAAVAVFVAKFVG"
     gene            1148..2128
                     /gene="btuC"
                     /locus_tag="ACHXOL_003884"
     CDS             1148..2128
                     /gene="btuC"
                     /locus_tag="ACHXOL_003884"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR6810735.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            2191..2742
                     /gene="btuE"
                     /locus_tag="ACHXOL_003885"
     CDS             2191..2742
                     /gene="btuE"
                     /locus_tag="ACHXOL_003885"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR6810736.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            2742..3491
                     /gene="btuD"
                     /locus_tag="ACHXOL_003886"
     CDS             2742..3491
                     /gene="btuD"
                     /locus_tag="ACHXOL_003886"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR6810737.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            3569..>4028
                     /locus_tag="ACHXOL_003887"
     CDS             3569..>4028
                     /locus_tag="ACHXOL_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR6810738.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT          972 a          883 c         1115 g         1058 t
ORIGIN      
        1 gatgaaatca ataagaagtc gatgagtcgt gtcaaaaaac ataaagagcg gctgatggag
       61 attacgccag aaatcttctt ccgtttcttg tctgagaagg gggtctccag tgtctgccct
      121 tcatgtggat cgtcgcgatt atctgttcca gaaagtatgg atctttgctg ggataaaaat
      181 aagaaaccag aaaattttaa caatctaccc ctggaggaac agagtgagtt aattaaagag
      241 aacataaagc attatgtatc ttatgctttt ttgggtgatg ttaagagcat acctgatatg
      301 cgcaaaactt attacacgct gcattgcctg aattgtggtt acctgagcct ttatcgtacg
      361 tctgcggtgc tgaagtggtt ggagaaagac aaagcgcagg atggtgataa tgggtaatgt
      421 agcaagaaat cttttcggta attcagcagg tagtgtgtcg cattctgagc gtgatgtgct
      481 ttatcatggt ggcgatggcg gcagtagtgg aggcggaatg tcagataaac ttgaaaggcg
      541 aattgagcgg ctcgaaggtg atttatcgct aacaagaaac gaccttgcga cgcttgctga
      601 acgcactaca aacctctcaa ctaaagccga tgttggtgag gtgaaaggtg aactcaaagc
      661 agacatagca catctgaaag gtgatcttga atgcgatatt gcgaatctga aaggtgagct
      721 taaatctgat acagctaacc tgaaagagca gctcaaatca gacattaaca gcctgaaggg
      781 tgagcttacc gaagcgatgg ataaacgctt tgacaagatt atggatgaga tgaatcggcg
      841 gtttgacaag gttgatgata atacgaagtg gcgttggagt ggcattattg tgccagtgtg
      901 cacaaccatt ttcacagcgg cggttgctgt atttgttgct aaatttgttg gctgatgatc
      961 cacaaattga ccacatctct gttatttgtt gtggttggct gtgtttttgt gtgtctgtaa
     1021 gtctttgata attacctaac ttattgattt ttgtttgtgt ttatggccgc tctgcggcct
     1081 tttttctttt cactgtcgaa gagtcaccgt aaaatcaacg ccatgacact tcagcagaac
     1141 ggataccatg ctgacacttg cccgccaaca acagcgacaa aatattcgct ggttattatg
     1201 cctgtcagtt ttgatgctgc tggcgcttct cttaagcctt tgcgccggtg aacaatggat
     1261 ttcgccaggt gactggttta ctcctcgtgg cgaactgttc gtctggcaaa ttcgcctgcc
     1321 acgtacgctg gctgtattgc tggttggtgc ggcgctggct atatccggcg ctgtaatgca
     1381 ggcgttgttt gaaaatcctc tggcagaacc tggactactt ggcgtctcta acggcgcagg
     1441 cgtggggctt atcgccgcgg tattgcttgg gcaagggcaa ctccccaact gggcgctagg
     1501 gctgtgtgcg attgctggcg cgcttatcat cactttaata ctcttacgtt tcgcccgtcg
     1561 tcatctttcg accagtcggt tattgctggc tggcgttgca ttagggatta tctgtagcgc
     1621 actaatgacg tgggctatct acttttccac ctctgttgat ttacgtcagc tgatgtactg
     1681 gatgatgggc ggttttggcg gcgtagactg gcggcaaagc tggctgatgc tggcattgat
     1741 ccccgtgttg ttgtggatct gttgtcagtc caggccgatg aatatgttag cacttggcga
     1801 gatctcggcg cggcaactgg gtttacccct gtggttctgg cgcaatgtgc tggtggcagc
     1861 gaccggctgg atggttggcg tcagtgtggc gctggcgggt gctatcggct ttattggtct
     1921 ggtgatccca catattcttc ggttgtgtgg tttaaccgat catcgcgtat tacttcccgg
     1981 ctgcgcgctg gcaggggcga gcgcagtgct gctggccgat attgtagcgc gcctggcatt
     2041 agctgccgca gagctgccta ttggcgtggt caccgcaacg ttgggtgcgc cggtgtttat
     2101 ctggttattg ttaaaagcag gacgttagcc gcaaaaagac ggtctatgat taaaagctaa
     2161 gatttttact gaccacaccc aggagaaacg atgcaagatt ccattctgac gaccgtagtg
     2221 aaagatatcg acggtgaagt gaccacgctg gagaagtacg ccggtaatgt gctgttgatt
     2281 gtcaatgtcg cctcaaagtg tggcttaacg ccgcaatatg agcagttgga gaatattcag
     2341 aaagcctggg ccgatcgagg ttttgtggtg ctgggattcc cgtgcaacca gtttctggaa
     2401 caagaaccgg gcagcgatga agagattaaa acttactgta ccaccacatg gggggtgacg
     2461 ttcccgatgt tcagtaagat tgaagttaac ggcgaaggac gccatccgct gtatcaaaaa
     2521 ttgattgccg cagcgccgac cgcagtcgcg ccggaagaga gcggattcta tgcccgtatg
     2581 gtcagcaaag gtcgtgcacc gctgtaccct gatgatattt tatggaattt tgagaaattt
     2641 ttagttggca gggacggatt ggtcatccag cgtttttccc cggatatgac gccggaagac
     2701 cccattgtga tggaaagcat taaactggcg ttggcaaaat aatgtctatt gtgatgcagt
     2761 tacaggatgt tgcggaatct acccgcctgg ggccactttc tggcgaggtt cgggctgggg
     2821 agatcctgca cctggtgggg ccgaatggcg cgggtaagag taccttactg gcacgaatgg
     2881 ccggaatgac cagcggtaag ggaagtattc agttcgcggg gcaaccactg gaagcatggt
     2941 ccgcaacaaa actcgcgctg catcgcgcct atctttcaca acagcagacg ccgccgtttg
     3001 caatgccggt ctggcactat ctgacactgc atcagcacga taaaacgcgt accgaactac
     3061 tgaatgatgt cgcaggggcg ctggctcttg atgacaaact cggacgtagc accaatcaac
     3121 tttccggcgg tgaatggcaa cgcgtacgtc ttgctgcggt ggtgctgcaa atcacaccac
     3181 aagccaatcc cgcaggccaa ttgctgcttc ttgatgagcc gatgaacagt cttgatgttg
     3241 cgcaacaaag tgcgttagac aaaattctga gcgctctgtg tcagcaagga ctggcgattg
     3301 tgatgagcag tcacgatctc aaccacacat tgcgtcatgc gcatcgggcg tggttgctaa
     3361 aaggtggaaa aatgctggcc agtggacgca gagaagaggt gctcacgccg ccaaatctgg
     3421 cgcaggccta tgggatgaat tttcgccgtc tggatatcga aggtcacaga atgctgattt
     3481 cgaccatctg atttcattag tatcttgaaa atcgggtaat tacgacgcta aattaatgca
     3541 agaataaaaa acagaggaac cgtacggaat gcgtttctgc cttattttga tcacagcact
     3601 gtttctggcc gggtgtagcc accataaagc accgccgcca aatgccagac tttctgattc
     3661 gattaccgtt attgccggtt tgaacgacca gctacaaagc tggcatggca cgccgtatcg
     3721 ttatggtggc atgacgcggc gcggtgtgga ctgttcggga tttgtggttg taacgatgcg
     3781 cgatcgtttc gatttgcagc tgccccgaga aaccaaagaa caagcctcta tcggcacgca
     3841 aattgataaa gacgagttgc tgcctggtga cctggtcttt ttcaaaacgg gttccggaca
     3901 aaatggtttg catgtaggta tttacgatac caacaaccaa tttatccacg cctctaccag
     3961 caagggagtg atgcgttcct cacttgataa tgtctattgg cagaaaaatt tctggcaggc
     4021 gagacgaa
//
LOCUS       DAZRUF010000289         4023 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZRUF010000289 DAZRUF010000000
VERSION     DAZRUF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.292"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..590)
                     /locus_tag="ACHXOL_003888"
     CDS             complement(<1..590)
                     /locus_tag="ACHXOL_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR6810739.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="ACHXOL_003889"
     CDS             complement(583..993)
                     /locus_tag="ACHXOL_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR6810740.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="ACHXOL_003890"
     CDS             complement(999..1532)
                     /locus_tag="ACHXOL_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR6810741.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2586)
                     /locus_tag="ACHXOL_003891"
                     /pseudo
     CDS             complement(1510..2586)
                     /locus_tag="ACHXOL_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
     gene            complement(2583..3974)
                     /locus_tag="ACHXOL_003892"
     CDS             complement(2583..3974)
                     /locus_tag="ACHXOL_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR6810742.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRESLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
BASE COUNT          942 a         1196 c          982 g          903 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctagt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcgc
     2041 cgtcgccgct ttacctgttc cgggttttcc cagaaccagt cgcccgcatt catcgtcata
     2101 agccagcgtc tgaacctgcc ccagtaaccg gttaagacaa tcggcaacgg tttcgccgtg
     2161 ttccggctgg gcatcaatca ccgctgtctg cggcacgcca gcatcaacaa cggtgatgcc
     2221 aaatggcgca gccagttcac tgactattct gagcaggttt tttccgctct gctggagtgg
     2281 caaagcagag cagtcaacca gatcggctgt tttgcttcgc ccgacaattc ccatgctgac
     2341 gctgctggcg tcataacgaa gcggtagtgc ctccacatat ccggtgagca caggctcatc
     2401 cccgataagc acttcaacca gctcaccatt ttttatccgg ggttgataat cccggcttcc
     2461 gggccagcgg gtggtaatgg caacattaaa atcccgggca atacggttaa tgcccgcact
     2521 gatacggacg gatgtccagc cgccccattc gcgaccggaa acccgaagta aaacggtatc
     2581 attcatctga cgggtaccct taatgccctg accggaacaa agcccggatg agagatggca
     2641 tttcgatcca ggatatcagt ttcacgggac gcatcgtcgt accatgacgc agccagaaca
     2701 agcgcaggca gaacttccgc tggcgttcgc tctgcggttt cctccgtctg aaccagacgc
     2761 gcctgaatat cccggttcag ttctgtacgt aatgacgtca gctgaaaaaa cagcctgtca
     2821 tccgccgtac gcctgagctc ctgttcaatc gccgcgttca gcgattcgcg tatgatggtg
     2881 agattttccc gcgtgggtgg ttgcgctctc tcatcctgtc ctgtgctggc tgccacgctg
     2941 tcaagtgccg gatgagaaac gtgaataata tcggactgac gttcagtgga accaccaaca
     3001 gccacaaccg cctgctgatt tttcaccagg cttccgggtt gtggcagtga agtgaccgcc
     3061 cttgtggctt cgctgattgc cgtcgtccgg atgacagcag ccacaaggtt tgtctgctgt
     3121 ttttgcctga taaccgatgc ggaatctgtg ggccacaccg cacgcggtgc cagtcctggg
     3181 tccagcgtaa tgccggacat tgtcgttatg gactgcacca gatcctgtgt gttatccacc
     3241 agccgggttc cggcccgcca ggtatcctgc aacgtatgca caaaatcact ggcaaccgac
     3301 ggcggcatca gaatgacgga taaatcgccc tgcagtagtc tcgctccggc agaaacagcc
     3361 gaattaacca ttttgaaagc tgtctgaacg gttcccagca tatcggtcgt ccgggcaata
     3421 acatcgttct gaataaagtc cggtattccc gcgagatcaa aatcgccgaa catgtcttca
     3481 atcaactcat ccaggaatac ggacgactcc tccagttttc tggcggttgc agctccggcg
     3541 accggaaatg acagttcccc gctctcaaca aactgaaatg aaacccggca catacggcct
     3601 tcagtgccgg agtgagaaac ggtcacctgc ccgtcaatac agccctgcat ttcaccgaac
     3661 tgcggatgga tcagcgtccc cggcccggcg gtttcaatcg ctgtaatcag cctgtcgcgc
     3721 tgctctgcgt aatcatcacc aacgagatac gcattaatcg tcagccgtcg cgtggcacgc
     3781 ccgagatctt ccgtgtacgg tttgtcacgg tttgggtatt catggacctg aacgcggcgt
     3841 ccgaacgaac cctcgtcgct ctccaccgaa aacggaacgc cacgaaatga agcgtcgtaa
     3901 agattatcgc gccaattgat acctgaagat gcagaaaaga gtgaagaaag agaaggtaaa
     3961 gaaggtaagt ccatactgtc tccactataa aaaaaccgcc aatttctggc ggcttatata
     4021 tta
//
LOCUS       DAZRUF010000290         4023 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZRUF010000290 DAZRUF010000000
VERSION     DAZRUF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.293"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..366)
                     /locus_tag="ACHXOL_003893"
     CDS             complement(<1..366)
                     /locus_tag="ACHXOL_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR6810743.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSE"
     gene            complement(381..1127)
                     /locus_tag="ACHXOL_003894"
     CDS             complement(381..1127)
                     /locus_tag="ACHXOL_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR6810744.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLADAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            complement(1134..1442)
                     /locus_tag="ACHXOL_003895"
                     /pseudo
     CDS             complement(1134..1442)
                     /locus_tag="ACHXOL_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
     gene            complement(1821..2333)
                     /locus_tag="ACHXOL_003896"
                     /pseudo
     CDS             complement(1821..2333)
                     /locus_tag="ACHXOL_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
     gene            complement(2346..2768)
                     /locus_tag="ACHXOL_003897"
     CDS             complement(2346..2768)
                     /locus_tag="ACHXOL_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR6810745.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(2765..3007)
                     /locus_tag="ACHXOL_003898"
     CDS             complement(2765..3007)
                     /locus_tag="ACHXOL_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR6810746.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            3104..3523
                     /locus_tag="ACHXOL_003899"
     CDS             3104..3523
                     /locus_tag="ACHXOL_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR6810747.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRIKDNPPPE"
     gene            3829..3984
                     /locus_tag="ACHXOL_003900"
     CDS             3829..3984
                     /locus_tag="ACHXOL_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR6810748.1"
                     /translation="MNTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT         1007 a         1080 c          894 g         1042 t
ORIGIN      
        1 ttcgctcttc cggcactcgc gacagattat attcaggcgc ctgtcgtagc ggcgtatttc
       61 tccgtctggt aatgaccaga taaggtcagg atcaaccaca accggtttct tcacctttac
      121 cctcgatagc tttttgcggg cgttttgcca gtctttatga gcctgttcag agggaaatac
      181 cccgtagcca gaattatata catcaccact tgcgaccagt tccatgcata agcgaccgac
      241 agacgcacga ctgatacctg tttcatctga caactgccga atcgtgaccc gaccgtcctg
      301 atgcacgagt tccacaattc gcgtcttcag ttcttcccgc tgttcgggag taaaaggttt
      361 cgccataaat cctcctgaaa ctacttaaca accctcgaat ggctaacatt cggacgccag
      421 ctctcccagt taaaattcac ccagcgcccg ccgttcatga ccatgcgatc catcacccgc
      481 tcgccgagaa gtgtattcat cgctgcatgg ttaatatttg tcagcatccc cacaccgcgt
      541 aacgatgccg tccggcgatc aacaatctgg ttcagtacca cctgctcgtt tttcgtatcc
      601 cgctgcatgc caatttcatc caggaccagc aggtcaacac cacaaagctc ctgtaaaaat
      661 ttttcaccgg atttgccgtt gtcgtagctc tcatgcaaca cgctcatgac atcggacacg
      721 gtgacgataa tcacgctacg ccccttcgcc atcagccggt tgccaatcgc gtctgcaaga
      781 tgattttttc cggtgccggg cttaccgctg aacacaaaat tcgtacagcc tgtcatcagt
      841 tcgtcagcta tggatttcgc ctgactcagt gcatgtcgct gaccgtcgtt ctgcgcccgg
      901 taattcgcaa acgaacactt ccggtgcaac ggctggatgc cggagcggtt caggattttt
      961 tccacccgca actgacgatt caggcggttg atctcctcgc aacgtttctg gccttcagca
     1021 agttgccact cgcgccactc cgcaaccgtt ctgaatgggg cggttacatg tggtggggtc
     1081 agtctgcgga tacgctccag aacgcctcct gtcgcaatat ttttcatggt ctgttacccc
     1141 ctgaagcctg gcgggatcgc actgtccggc aacgagacgg tgttaacctg tcggagcaac
     1201 gtctcaggcc gaacaccttt cggcgcgaac agaccctggt attcattggc gatgctgtgt
     1261 cgaatcacct gctcaggtgt aaaaccctgc tgacggaatt tttccagttc ccgtatcgcc
     1321 ccgttagcgc cctgctccgt tcgaatcggt tttcgcaatg cctgcctgaa ctggacccac
     1381 tcatgccaga gtgtttctgg caaccagtcg ggcagatcaa ctgacaacgg gtcaaatttt
     1441 ttctggcgaa cagccccacc gataaccaga attccctgct cggtgtgcct tgcgatatca
     1501 ctgcgccgca agacacatat cccggtttca acagcaccga ttttgagcag tgatttctgt
     1561 cgcggagaat ttcggtcaag ctcgctccca caccggacac cgttcctgtc agtcacgacg
     1621 atatcaatcc gccctccgca accgtcacca cgctctggca ccggatactc acgacagaca
     1681 tccaggccag cagcctgcaa cgcaaccacc gcagaattac aaaattccgc tgcagtaccg
     1741 ccagataatc tcccctcaag cactccagcc acaaaatccc gaaactccct tccggaaggg
     1801 aagggtttgg gatgggttag atctgttttt agatctttat ctgtatcttt attagttgcc
     1861 tttgtgttgg catcatgttc gaacatcact ccaacatctg tttgaacacc tgttaaattt
     1921 ctctcttgtt ttgtttgaac atctgcttcc tttctgcttc ttctggcctg aacagatgct
     1981 tttcctgcgg ctgatttttt ggttaatttt tctctgacag atgccagatc ttcctcaatc
     2041 cgaagatgca tccattcgtc gccgttatca caaaaaaact cccgcaagga tggttcaaca
     2101 tcaacccatc gctcgttagt cagacgggca atttttgcca acctgttttt gggtattggc
     2161 tttcctgttt gccagtaatt gaacatcagc aacaaatacg cgccgtgctc ctctgctgac
     2221 aaatgcatgg tgtcagccag gtaatcagct atgtacagtt gcatgtatgg taatgcggcc
     2281 ataattgccc cgtatgatgc tgcccggttg cttagaataa gcacaaacag catggaaact
     2341 tttgcttaat gaacaatgac agaatcgtcg gaagacccgc cgccgctgaa atgcgctttc
     2401 cggtaaacgg cctggactgc atcatcatgc gcatcaattg ccgtactcaa cgcttcctgc
     2461 gccgccagta atgcacggcg ttccagggta tcgaagatgc agagtcggtg acgcagctcg
     2521 cgcggaagaa ttgccagaac cgcagggatc agtttctgaa ttttttccct ttgcgctttc
     2581 gtttcacctt tcaaccaacg gtgatagata ttctgctgat tgttccagtc cttgcctggt
     2641 acaaggggca attcgccgcc cccctggcgc agatattctt cagtaattgc gttagcgacc
     2701 cacgcctgcc ctttttcggc tgccagggct aacaacactg attcgatgtg ctcatgcctg
     2761 attttcatga atcaaccgct cctatgctgt tttcgctatg cttaccgtct ggggggaata
     2821 catcgtcaag tccacaatga gcgccaagcc gattaagggt agaaacaatt tttctgcact
     2881 cctctagtcc aggggtacga aaatttgctt cgtaatttgc cagtcggctt tgtatccacc
     2941 ctaactgaac agcaagttgt ctttgagaca gcccaagctg ttttcgatat gttgaaattt
     3001 tgttcattga aaacctccga tgacaatttt aaacacacct tgtgttatat agccaagctg
     3061 ttttgtgttt tatgtaaatc acgattcgtg atacaaggat gcaatggaaa aagaaaacga
     3121 aaaaattgcc gctagtaggc tcaatgacaa aattgcaatg cgtcttaaag agcgcaggca
     3181 gaagcttggt ttatctcaag gaaaacttgc tgaaatctgc ggatggacgc aatcgcgtat
     3241 aggtaactat gaggcgggca gcagaaatgt tggagtacat gacgctgtcg tattgggaaa
     3301 ggcacttggc atatctcctc ctgagctcct ctttggagaa caggaatctt ctgaattgtg
     3361 gttaaatgaa tcccaacgaa aacttcttga gttgtttaac cagctaccgg gctcagaaca
     3421 acaacgaatg attgagctat ttgaagtccg gctaaaagaa atcgatgagt atgtagaaaa
     3481 atatttgaga ggcaggatta aagataatcc cccaccggaa taatgatctt gctatcacag
     3541 taatatgcca atcagcccgc tatcagcggg cttttttgta gcatcatcat atgacactca
     3601 ccataaaaca cattgcgtgt tgacacaaga aaacaaattg tgtttaataa gcatatccaa
     3661 acaacgcccc accagagaac ggcaggacaa tacctcgagt tatccagtca ctgaacaggg
     3721 ctaagtagcc agcctgaggc atacgaacat gacggcagtt gttgattgat acaaagcgca
     3781 gtagataaaa cgttccgcca cccggcgtta aggggaaatg gggtcaacat gaatactatc
     3841 gatcttggca acaacgaatc tctggtatgt ggcgtgtttc ccaatcaaga cggcacattc
     3901 accgccatga cgtataccaa aagcaaaacg tttaaaaccg aagctggcgc acgtcgttgg
     3961 ttgggaagac attcaggtga gtaaaatgaa cgagacagaa ttaaaacacg ttatcgctct
     4021 act
//
LOCUS       DAZRUF010000291         3955 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZRUF010000291 DAZRUF010000000
VERSION     DAZRUF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.294"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..512
                     /gene="ykgH"
                     /locus_tag="ACHXOL_003901"
     CDS             <1..512
                     /gene="ykgH"
                     /locus_tag="ACHXOL_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR6810749.1"
                     /translation="KINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVK
                     NREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQIPSAINSELY
                     TKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMV
                     DEYQQNSGQ"
     gene            complement(755..1450)
                     /locus_tag="ACHXOL_003902"
     CDS             complement(755..1450)
                     /locus_tag="ACHXOL_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR6810750.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1443..2870)
                     /locus_tag="ACHXOL_003903"
     CDS             complement(1443..2870)
                     /locus_tag="ACHXOL_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR6810751.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2881..3600)
                     /locus_tag="ACHXOL_003904"
     CDS             complement(2881..3600)
                     /locus_tag="ACHXOL_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR6810752.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1040 a         1051 c          826 g         1038 t
ORIGIN      
        1 taaaaataaa ccagacaaca ccaggcatac ttgttaactg tgatagcaat aataactttg
       61 cctgtcagac cacaatgact gaagatgtta tacagcgaat tactacattt tttcacacca
      121 gcccagatgt caagaacaga gaaataaagc tggaatggtc aggagataag agagatttac
      181 caactgctga agcggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      241 aataccataa tggcaggcaa gttctcgatg agatacaaat accttcagca attaacagtg
      301 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      361 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      421 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      481 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      541 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      601 tttttcactg taaatatcct ggcattacta atgccaggat atttacagaa gagcagcata
      661 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      721 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
      781 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
      841 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
      901 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
      961 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc gacggcggaa aaaagaacca
     1021 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1081 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1141 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1201 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1261 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1321 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1381 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1441 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1501 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1561 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1621 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1681 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1741 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     1801 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     1861 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     1921 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     1981 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2041 ggtcctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2101 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2161 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcactagg
     2221 cataccgaac ctgtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2281 aggaaatctt cgcggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2341 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2401 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2461 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2521 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2581 atgtagcggg tagcgtcttc tttggttttt gcaaaccaga cgtgaccgcc gttttgcgtc
     2641 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2701 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     2761 tttgccccaa tacgctgctg cgcgtttgcc accgctttgc gcatgatcgg atcttcaatt
     2821 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     2881 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     2941 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3001 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3061 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3121 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3181 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3241 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3301 acgtgatgcc cattcgggtt catccgccag atacgtcggg taacttttta cggcataggt
     3361 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3421 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3481 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3541 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3601 aactaatact ccattacttc atgcccattt atgcccgatg gttcccctcc ttccggggga
     3661 tcaatcatca ataaatatgg cgttcttttt taattggcta gcaacatacc acccctcatt
     3721 tgtgtgacaa atgataatct ttcatccaaa cttcaaatgc aataactaat tttatttctt
     3781 tcggcatcgc tcacatttta tggggaaatc gacgttcatt tttggcataa gaaatccatt
     3841 taaaaaggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     3901 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtc
//
LOCUS       DAZRUF010000292         3948 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZRUF010000292 DAZRUF010000000
VERSION     DAZRUF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.295"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            32..970
                     /locus_tag="ACHXOL_003905"
     CDS             32..970
                     /locus_tag="ACHXOL_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR6810753.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDNPPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1026..2315
                     /locus_tag="ACHXOL_003906"
                     /pseudo
     CDS             1026..2315
                     /locus_tag="ACHXOL_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            2312..2605
                     /locus_tag="ACHXOL_003907"
     CDS             2312..2605
                     /locus_tag="ACHXOL_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR6810754.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            2770..3948
                     /gene="fadK"
                     /locus_tag="ACHXOL_003908"
                     /pseudo
     CDS             2770..3948
                     /gene="fadK"
                     /locus_tag="ACHXOL_003908"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
BASE COUNT          930 a         1003 c         1089 g          926 t
ORIGIN      
        1 gccctgaact gaagcctacg gagaagaaac gatgagtcaa ttaaacagcg tctgggtctt
       61 tagcgataat cctgaacgtt atgctgaact gtttggcggc gcgcagcaat ggggccaaca
      121 ggtgtatgcc attgtacaaa ataccgacca ggcgcaggca gttatgcctt atggtccaaa
      181 atgtatttat gttcttgagc aaaatgatgc gctgcaacgt actgaaaatt acgccgaaag
      241 cattgctgcc ctgctgaaag ataacccccc cgctatgttg ctgctggccg cgacgaaacg
      301 tggtaaagcg ctggcagcac ggttaagtgt gcaactgaat gcggcgctgg tgaacgatgc
      361 cacggcagtg gatattgtcg atggtcacat ttgcgccgaa caccggatgt atggcgggtt
      421 agcgttcgct caggaaaaga tcaacagccc gctggcgatc attacccttg cacccggtgt
      481 tcaggaaccg tgcaccagtg atacctctca tcagtgcccg acagaaacgg taccttatgt
      541 tgccccgcgt catgaaattc tctgtcgcga acgccgtgcg aaagcggcaa gcagcgtgga
      601 cctgagcaaa gcaaaacgtg tggttggcgt cggtcgtggt ctggcagcgc aggatgacct
      661 gaaaatggtc cacgaactgg cggcggtgct gaatgctgaa gtcggctgtt cacgtccaat
      721 tgccgaaggc gagaactgga tggagcgtga acgttatatc ggtgtctccg gcgtgttgct
      781 gaaatccgat ctctacctga cgctggggat ctccgggcag atccagcata tggttggcgg
      841 caacggcgca aaagtgattg tcgccatcaa taaagataaa aatgcgccaa tcttcaatta
      901 tgccgactac ggtctggtgg gcgatatcta caaagtcgtc cctgccctga ttagccagtt
      961 gagccgctaa ttcccctttc aacaacatct cccgctgtaa acgcagcggg aagggagcat
     1021 aacgcatgtc ggatgacaaa tttgatgcca ttgtggtcgg tgcgggcgtt gctggtagcg
     1081 ttgccgcact ggtcatggcg cgagccgggc tggatgtcct ggtgatagaa cgcggcgaca
     1141 gtgccggatg taaaaacatg accggcgggc gtctttatgc ccacacactt gaagcaatca
     1201 ttccaggctt tgcagtatca gcgccggtag aacgcaaggt cacacgcgag aaaatctcct
     1261 tcttaaccga agaaagcgcc gttaccctcg attttcaccg cgagcagcca gatgttccgc
     1321 aacacgcatc ttataccgta ttgcgtaatc gtctggaccc gtggttgatg gaacaagccg
     1381 agcaggcggg cgcgcagttt atcccaggcg ttcgcgtcga tgcgctggtt cgtgaaggaa
     1441 acaaggtaac tggcgtgcag gctggggatg atattctcga agcgaatgtg gtgattctgg
     1501 ctgatggcgt taactcgatg cttggccgct cgctgggaat ggttcccgct tccgatccgc
     1561 atcattacgc tgttggtgtt aaagaggtta ttggcctcac accagaacag atcaacgatc
     1621 gctttaatat tacgggcgag gaaggtgccg cctagctgtt tgccggttcc ccttctgacg
     1681 gcctgatggg tggcggattc ctctatacca acaacgattc cgtatcgttg gggctggttt
     1741 gtggattggg tgatatcgcc catgcgcaaa aaagcgtgcc gcaaatgctg gaagatttta
     1801 aacaacaccc cgccattcgc ccactaatta gcggcggcaa actgcttgaa tattccgcgc
     1861 atatggtgcc ggaaggcggt ctggcgatgg tgccgcaact ggttaacgag ggcgtgatga
     1921 tcgttggtga cgccgcaggc ttctgtctga atttgggttt tacggtccgc ggcatggatt
     1981 tagccattgc atcggctcag gctgccgcca caacggtgat cgccgccaaa gaacgcgagg
     2041 atttctccgc cagcagtctg gcgcaataca agcgtgagct ggaacaaagc tgcgtcatgc
     2101 gtgatatgca gcattttcgc aagatcccgg cgctgatgga aaacccgcgc ctgtttagcc
     2161 aatacccacg aatggtagcc gacatcatga acgagatgtt caccatcgac ggcaaaccaa
     2221 accagccggt acgaaaaatg atcatgggac atgcgaagaa aattgggctg atcaacttgc
     2281 tgaaagatgg cattaaggga gcaaccgcgc tatgagccag aacgctacgg ttaacgtcga
     2341 catcaaatta ggcgtcaata aattccatgt tgatgagggc cacccgcata tcattctggc
     2401 ggcaaatccc gacatcaatg aattccgtaa attaatgaaa gcctgccctg ccggacttta
     2461 taagcaggat gacgccggca acattcattt tgattctgcc ggttgtctgg agtgcggcac
     2521 ctgtcgggtg ctgtgcggta acactattct cgaacagtgg caatatcccg caggcacctt
     2581 cggtattgat tttcgctacg gctaatcatg catcccacag gcccgcatct cgggcctgat
     2641 gttctgtttc gagagtccaa catgaaagtg acattaacgt tcaacgaaca acgtcgtgcg
     2701 gcgtatcgtc agcaagggct atggggcgat gcttcgctgg ccgattactg gcagcagacc
     2761 gctcgtgcga tgccagacaa aattgccgtg gtcgataatc atggtgcatc gtacacctat
     2821 agcgcgctcg atcacgccgc gagctgtctg gcaaactgga tgttagcgaa gggtattgaa
     2881 tcaggcgatc gcatcgcatt tcaactgcca ggctggtgtg aatttaccgt tatctatctt
     2941 gcctgcctga aaaccggtgc ggtttccgtg ccgctgttgc cttcctggcg ggaagcagaa
     3001 ctggtgtggg tgctcaataa gtgtcaggca aaaatattct ttgcaccgac gttgtttaaa
     3061 caaacgcgtc cggtagattt aatcctgccg ctgcaaaatc agcttccaca actacaacaa
     3121 attgtcggcg tggacaaact ggctcccgcc acctcttccc tctcattaag tcagattatc
     3181 gccgacaata tcccactgac cacggcgata acgacccacg gcgatgaatt agctgcggtg
     3241 ctgtttacct ccggaaccga gggtctgcca aagggcgtaa tgctaacgca taacaatatt
     3301 ctcgccagtg agcgggctta ttgcgcgcgg ctgaatctga cctggcagga tgtctttatg
     3361 atgcctgcgc cacttggtca cgcaacggga tttctgcatg gcgtaacagc accattttta
     3421 attggtgctc gcagcgtgtt gttagatatt ttcactcctg atgcatgtct cgcgctgctt
     3481 gagcagcagc gttgcacctg tatgctcggc gcaacgccgt ttgtctatga tcttttgaat
     3541 ttactagaga aacaacccgc agacctttca gcgctgcgct tctttctttg cggcggtacc
     3601 acaatcccca aaaaagtggc gcgcgaatgc cagcagcgct ggcattaaat tattaagtgt
     3661 ttatggttcc acagaaagtt cgccgcatgc ggtggtgaat ctcgatgatc ctttgtcgcg
     3721 ctttatgcac accgatggtt acgctgccgc aggtgtagag attaaagtgg tcgatgacgc
     3781 acgcaagacc ttaccgccag gttgcgaagg tgaagaagcc tcgcgtggcc ccaatgtgtt
     3841 tatggggtat tttgatgaac ctgaattaac cgcccgtgcg ctggatgaag aaggctggta
     3901 ttacagcggc gatctctgcc gtatggatga ggctggctat ataaaaat
//
LOCUS       DAZRUF010000293         3941 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZRUF010000293 DAZRUF010000000
VERSION     DAZRUF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.296"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(130..798)
                     /gene="artM"
                     /locus_tag="ACHXOL_003909"
     CDS             complement(130..798)
                     /gene="artM"
                     /locus_tag="ACHXOL_003909"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR6810755.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(798..1514)
                     /gene="artQ"
                     /locus_tag="ACHXOL_003910"
     CDS             complement(798..1514)
                     /gene="artQ"
                     /locus_tag="ACHXOL_003910"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR6810756.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1521..2252)
                     /gene="artI"
                     /locus_tag="ACHXOL_003911"
     CDS             complement(1521..2252)
                     /gene="artI"
                     /locus_tag="ACHXOL_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR6810757.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2270..2998)
                     /gene="artP"
                     /locus_tag="ACHXOL_003912"
     CDS             complement(2270..2998)
                     /gene="artP"
                     /locus_tag="ACHXOL_003912"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR6810758.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3216..3731)
                     /locus_tag="ACHXOL_003913"
     CDS             complement(3216..3731)
                     /locus_tag="ACHXOL_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR6810759.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT          987 a         1035 c          994 g          925 t
ORIGIN      
        1 tttatgcact atgtaggccg gataaggcgt ccacgccgca tccggcacag gcaccgtgct
       61 gatgtctgat gcgacgctgg cgcgtcttat cagacctaca aacccccccg gcgaatgtac
      121 gcagctacat taattccgcc gttcgaatgc cagtgctttg cgctcgatca gacgcatcat
      181 cagcgtcagc aggccgttga cgaccaggta aataatccct gccgcaccga acaccattac
      241 atcgtaggtg cgtccgtaca acaactggct gtatcccatc acttccatca gcgtaatggt
      301 gtaagccaga gaggtacttt tgaacaccag caccacttcg ttggaataag aagagagcga
      361 gcgtttaaag gcatacggca gcaggagcgc cagcgtatct tttttgctca ttcccagggc
      421 gctacaggac tgccactgac cttctgggat cgcacgaatt gcaccgtaaa acagctgcgt
      481 ggtatacgcc gcactgttca gcgataacgc aatcagcgca cataaccacg gttctgacaa
      541 caaatgccac agtgccggat actcctgcaa cgtcggaaac tggcccggcc cgtaataaat
      601 caggaagatc tgcaccagca gcggcgtacc ggtaaacagc gtgatataac cccgcaccag
      661 ccacaccagc accggcgttt tcagcgtcag gatgatggta aaaatcaatg ccagaatcag
      721 tgccacaatc agcgaggcaa cggttagcgt caggctggtg tgtagccctt tcatcagttc
      781 gggtaaatac tcaaacatta gctgggcctc cgctcaaaac gtgtcgcgcg caggtcaatg
      841 cgtttgagaa tgtactgact gagcagggtg atcaccaggt aaatcgccgc cgccacaatg
      901 taccaggtaa atggttcctg ggtacgagta gcgatgcttt ttgtttgcag cattaaatca
      961 ttcacactaa tcaaactgac cagcgcggta tctttcagca gcaccagcca ctggttaccg
     1021 aggccaggca gcgcatgacg ccacatctgc ggcatcacca gacggaaaaa gatagccgat
     1081 ttcgacagcc ccagcgcctg accagactcc cactgaccca ccggcaccgc tttcaacgcg
     1141 ccccgaagcg tttgcgaggc ataggcggca tacagcagtg acagagcgat gacaccacaa
     1201 aggaacgggc taacgtcgaa gttctcaatg tccatctgca ctgggatctg cacgaaccca
     1261 agattgatag tgaagccatc cgaaagcgtc agcagcagct gcgaggagcc aaaatagata
     1321 aacagcacca ccagaatttc tggcaggcca cgcagaatgg ttaccagcgc tgaacctgcc
     1381 cacgcgacag gacgccattt tgccgactcc cataccgcaa agaacatcgc cagcgccagc
     1441 ccgacaatca atgcacaaac ggcaaggccg acggtcatcc cggcggcgct tgctaaagga
     1501 aaaaattcat tcatcaggaa ttacttctgg aaccatttgt tgtagatggt ttcgtaagtg
     1561 ccatctttct tcactttttc cagcgcagtg ttgagtttct gctgcagctc agtgttgccc
     1621 tgacgtaccg cgatgccgag gccagtgccg aagtaatctt tatcggtcac tttgtcgccc
     1681 accgccgcca gtttcgggtt atctttcagc cactcagtga ccactgcggt gtcaccgaag
     1741 acgccgtcga tacgcccgtt ttgcagatcc agttttgcgt tctggtagct gtcatacgga
     1801 acggtagtga tttccgggtg cttatccata atgaatttct ggtgtgtcgt cccgttctgt
     1861 acgccgactt ttttgccttt cagctgatca acactggtgt atttgccttg ctgacccaca
     1921 aacagggcag agttgtcata gtacggggtg gtaaacagca cctgcttttc acgctccgga
     1981 gtgatatcca tgcccgccat cacggcttct acgcgacgga atttcaggct tgggatcagg
     2041 ctgtcaaacg cctggttaga gaaagtgcag gttgcatcaa tctctttaca cagcgcttgt
     2101 gccaggtcga cgtcaaaacc aacgatctgg ttgtttgcat caatcgattc aaacggagga
     2161 taggatgctt cggtagcaaa acgaatggtt tcggcagctg tggcggaaag actaaaacct
     2221 gcaattaacg cggcaatcag aacttttttc attgttgtta tcccgaatct tagtgagaga
     2281 gatagttttt aaatgcttcg gtttgcggct cggtaaagca gctcgcgtcg ccttgttcta
     2341 cgatatgacc attttccata tacaccactc ggctggcggt tttacgcgcc acttcaactt
     2401 cgtgggtgac gatcacctgg gtaatattcg tttctgccag ctcacgaatg atgctgacga
     2461 tttgtgccgt aatttccggg tccagtgcgg cggtcggttc atcgaacagc agtacctgcg
     2521 gttccatcat caacgcacgg gcaatagcaa cacgctgctg ctgaccacca gaaagatgca
     2581 gcgggtaacg atcgctataa ggtttgagac gcagacgttc cagcagtttt tctgcacggg
     2641 ccagcgcctg atctttactc aaccccagta cacggcaggg cgcttcaatc aggttttgct
     2701 gcacggtcag atgcggccac aggttgtatt gctgaaacac catgccaacg ttacgacgca
     2761 aatcgcgaat cgctttgtca gagggtgttt tggtgaaatc gaaatggttg cctgcaatgt
     2821 tgagcgtacc ggagcgtggc atctcaagca gattgagtac acgcagcagc gagcttttac
     2881 ccgcgccgct ggggccaagt aacaccagcg tttcgccctg tgggcaatcc agcgtgatat
     2941 cgaacagcgc ctgatgcgcg ccgtagaagc aattaatgcc gtttaattga atactcattg
     3001 acactcgtat actggcagtc tgatagctat tgaggtcgaa gatagtacct ttgacagaat
     3061 aattatgcaa tatttctgct ttaaaagtta aaagcaaagc gcattattca ataaacatag
     3121 cacaaaataa cgggggcggt ggtcggcgag cataaatgtc ggcattcctc acgaaatgcc
     3181 ggacaattta cggggtttat tggttgatca aggcgttagc gattctcgat ggactgacgg
     3241 agcgtacccg ccgtggcatg aacgctgccg cctaagtagc gcacatcgtc gatgacccaa
     3301 cactggcctt cctgaatcat taacacttca tcctgccaac cctggtcacc ctgtttgaga
     3361 tctacgcgca atggaatgtt acgggcatca cgattaggga tagtcgatgc actggcaacg
     3421 tgggcgctat ctggcaaggt ggttcgactg gagaacggat cgctgcttaa caattcacga
     3481 tgactattat cgcggctggc atcgctaaga agggttgcca gtttgtcact caaatagggg
     3541 cgcagggcgg taatgtcatt gctgcgatgt tgaatgcggt agtcatagaa ttgttgagcc
     3601 acgctgtcag gccccccctg gacacaggaa ccggtgcggg gaccgttatc tttataagct
     3661 ggcgtgacgg tggtacaggc actcagcagc agtgcacatg gaataagtag agatagtgaa
     3721 cggtagcgca taatcatttc cttattagct tgctgtctgc gtcacactac gaccaaacgc
     3781 tatcagatta tttaatacat tgtttttaat acaattctga aacaatctga acacctgatt
     3841 gggtttgagt ccattgctgg ctatccactc aaggttgctc gtagctgact tacttaaatt
     3901 acccaaacaa tagacagtgt aaaccatttg tgaggttgag c
//
LOCUS       DAZRUF010000294         3929 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZRUF010000294 DAZRUF010000000
VERSION     DAZRUF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.297"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..653
                     /gene="hprR"
                     /locus_tag="ACHXOL_003914"
     CDS             <1..653
                     /gene="hprR"
                     /locus_tag="ACHXOL_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR6810760.1"
                     /translation="DNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDDYALIILD
                     IMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPFSFSELLA
                     RVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLASRAGEII
                     PRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSFVAVKK"
     gene            653..2011
                     /gene="hprS"
                     /locus_tag="ACHXOL_003915"
     CDS             653..2011
                     /gene="hprS"
                     /locus_tag="ACHXOL_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR6810761.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(2119..2970)
                     /gene="hchA"
                     /locus_tag="ACHXOL_003916"
     CDS             complement(2119..2970)
                     /gene="hchA"
                     /locus_tag="ACHXOL_003916"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR6810762.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIDYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            3117..3527
                     /locus_tag="ACHXOL_003917"
     CDS             3117..3527
                     /locus_tag="ACHXOL_003917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810763.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            complement(3561..>3929)
                     /locus_tag="ACHXOL_003918"
     CDS             complement(3561..>3929)
                     /locus_tag="ACHXOL_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR6810764.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT         1113 a          757 c          928 g         1131 t
ORIGIN      
        1 aagataatca aaggacccag gagtgggtaa cgcaggggct ttccgaagcg ggttatgtca
       61 ttgatgccgt ttctgatggc agagatgggc tttatcttgc gctgaaggat gattatgcat
      121 tgatcattct ggatattatg cttccgggta tggatggctg gcagatctta caaacgttaa
      181 gaacagcaaa gcaaacccct gttatttgcc ttactgcaag ggattctgtc gatgacagag
      241 tcagagggct ggacagtgga gcaaatgatt atctggtaaa acctttttca ttttctgagt
      301 tgctggcaag ggttcgggca caattaaggc aacatcacgc tttgaattca acattagaaa
      361 tcagtggctt aagaatggac tctgtaagtc aaagtgtgag caggaacaat atcagtatta
      421 cactgacgcg caaggagttt cagttacttt ggctactggc ctccagagct ggcgaaatta
      481 tacccagaac ggttattgcg agtgaaattt ggggaatcaa ctttgatagt gataccaata
      541 cggtggatgt cgccattcgc aggctccgcg caaaagttga tgatcctttt cctgaaaagc
      601 taatcgccac aatccggggg atgggctatt cattcgtagc ggtaaaaaaa taatgaaaag
      661 attatctata accgtccgtt taaccttgct ttttatattg ctgctgtctg ttgctggcgc
      721 tggaattgtc tggactctct ataatggcct ggcaagtgag ttgaaatggc gcgatgatac
      781 aacactcatt aaccggacag cacagatcaa gcagttgtta attgatgggg taaatccaga
      841 tacgttacct gtatacttta accggatgat ggatgttagt caggatatct tgatcattca
      901 tggtgatggc atcaataaaa ttgttaaccg gacaaatgtc agtgatgaca tgttaaataa
      961 catacctgct agtgagacaa tcagcgcagc tggcatttac agaagcatta ttaatgatac
     1021 tgagattgat gctttacgaa ttaatattga tgaagtttcg ccatcattaa cggttactgt
     1081 ggctaaattg gcttcagcca gacataacat gcttgaacag tataaaatta atagcattat
     1141 aatttgcatt gtcgccattg tactttgctc agtattaagt ccgctgttaa tcagaacggg
     1201 attacgagag atcaaaaagt tgagtggtgt aacggaagcg ctgaattata acgatagccg
     1261 ggagcctgtt gaggttagcg cattaccgag agaactaaaa cctcttgggc aggcgttgaa
     1321 taaaatgcat catgctttag tcaaagattt tgagcgtcta agtcaatttg ctgacgatct
     1381 cgctcatgaa cttagaacgc ccattaatgc attactgggt cagaatcagg ttacgctcag
     1441 tcaaaccaga agtatcgctg aatatcaaaa aacaattgcc ggtaacattg aagagctgga
     1501 aaatatttcg cggttaacag agaacatact gtttcttgcc cgggcagata aaaacaatgt
     1561 tttggtgaaa ctggactcgc tttctctcaa taaggaagtc gaaaatttgt tggattatct
     1621 tgaatacctt tcagacgaga aagagatttg ctttaaggtc gagtgcaatc agcaaatctt
     1681 tgcggataaa attttactgc aacgaatgtt atcgaatctt attgttaatg ccattagata
     1741 ttcaccagaa aaatcgcgta ttcatataac cagttttctt gataccaacg gctatcttaa
     1801 tattgatgtc gccagtcctg gaacgaaaat tcatgagcct gaaaaactct tccgtagatt
     1861 ttggcgggga gataattcgc gtcattccgt aggtcaggga cttggtcttt ctttagtcaa
     1921 agcgattgcc gaattacatg ggggaagtgc tacgtatcac tatctcaata agcataatgt
     1981 gttccggatt atgttaccgc aaagaaatta acatagggct tcagtacgcc ataaatcaac
     2041 catgaaaaaa cgcagccacg tcgtgagtat taacgtggct gcgattgatt atgcgtttac
     2101 attcaaacgt aacagggatt aatccgcata agctgccagc atttcctgcg ccgccagttt
     2161 acccaacgca ttcgctgcga aaggactgtc gccggtgaga actttacggt ccttatgtac
     2221 tcgcccggtg atgtcgtcat taataatgtt catgcccatt ttcttcagct cttcgccgaa
     2281 gtaccaggtg agatgacccg gcatatagtc tatctctggc gtttgtttgt ctgcggcgtc
     2341 tgggaatgcg caaatggaat aaccattcag tgggttatcg ccgtggcgaa gcgccagaaa
     2401 agccgccggg ccgtggcaaa gggagataac aaaacggtca tttttgattg cccactgcaa
     2461 agcagcagct acgtcctggc tttctggtaa accaataagt gcgccatgac caccaggaac
     2521 aaagattgct gcatattcgc tatcagcgtt gaggctggcg acaacatccg cgagtttctt
     2581 cggattgcgg aacaacgatt tatgctgctc aaagaatggc attacttttt catctttgtg
     2641 cggcatagcc cagtattcaa acttggtcat cagaccggaa atggtcgcca cttcgaattc
     2701 gaaaccagca gcatggagat gatacaacgg cagcaacgtt tcaatcggat ggttaccggt
     2761 cgagaacagt tttccgttat cggttggcaa ataacgttcg tccgcggcga tcaccagaat
     2821 tttatgctta ccgcgatacg gttttggata gtccacgcca tcaagatcag agacgggact
     2881 ggtatattgg ctaagcgaat attctgaagg gaagaatgca ttatcttcag caatatcgac
     2941 ctgcggattt ttacttgttt gaacagtcat agtgtattcc ttattgttgc ttagttaggg
     3001 tagtcactat atttgcgact gagctggtcg ctatttttaa ccagtctgga aaattgagtc
     3061 aatatatcga aagcggtagt cggcgggtgt aagtatgtaa tgatgatgct gcactggtgt
     3121 tgaatattat tcacgccaaa ttttccttat tgagcaacgt cgtcgcaagt ggcctcaatg
     3181 attttagacc attatcaaag gtacggggcg gcggggagag atttagtgct gtgaaatatt
     3241 gtggtactga tgaatattta tatcgcggat tggtagagaa aagattattt catgcactat
     3301 ttttgcaaat atttctatat gggaagcgtg tttgttacgg attaattcat aaagaaatgc
     3361 atttgggtgt tggtttgaag agtcgctggt tgatgttatt gtgccaggag gggcagggta
     3421 aggtgctgta tcgatatgca ttggcaattg cgtgtgccgc agatggagat gaaatgccaa
     3481 taaaaaagcc tgaattcgat gagggacgaa ctcaggctag tgtttagttt tacagtaaca
     3541 cgccgcagca cgttggctga ttagaactgg tagactaagc ccacaccaac aacgtcatct
     3601 gttgcgatgc cattgttgtc atagaaatcg tcatctttat ccaacaagtt tattttatag
     3661 tcaacatatg ttgagaagtt cttattgaaa tagtaggtcg ctccaatttc aacatagttg
     3721 accagatcct gatcaacata ctcttgatga ccattgttgt tatagtcgtt accgccgagg
     3781 tctttgcctt ttgaataaac atatgcaaga gatgggcgca gaccaaaatc gaactgatac
     3841 tgtgctacag cttcaaagtt ctgggtttta tttgcgatac ccacattacc atatggtgtc
     3901 atattgcggg tttcagcata catcattgc
//
LOCUS       DAZRUF010000295         3911 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZRUF010000295 DAZRUF010000000
VERSION     DAZRUF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.298"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..3511)
                     /locus_tag="ACHXOL_003919"
     CDS             complement(<1..3511)
                     /locus_tag="ACHXOL_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR6810765.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQ"
BASE COUNT         1006 a          995 c          812 g         1098 t
ORIGIN      
        1 cctgacgcct ctcaagacta ttataaatag cctcaatctc acccttcagg ttatcaccgc
       61 aataaaccat tgatgtatcg tatcgttcat atcgcgcttc ccgtgtcgca ctaaaccagt
      121 tgaacgatcc cacacacagc aaaccatcat caccaataac aattttgcta tgaacacggt
      181 ttaccagctt tgtagcaata cccagcgcat tcagtttctc cagcgccgct ttaaagttct
      241 gctgcttctc ttttcgcttc tcaaaatcat tatgttcagt gttgtagctt ctgtcagtga
      301 ctatcgtgac gttaattcca cgtgaacacg ccgcaatcat ggaatcaaga aaaccggttt
      361 gctccagcct ttgccaggtc agccatggag aaattatcgt gatgtgttta ctggtatttt
      421 caaatgtctg attcaggaaa ttatcatgtt gctccacacc atgaagtgtg tagattttgg
      481 tcccggcggt ttttaaatcc ttacgctctt tataatcaaa agagagcgca ttcttctctg
      541 actcaaagag gtattttgcc agtaatcccc gtggcgatga ggctggctgg acctcaaaca
      601 ggtccatatc gccgaagacc agaaaactgt ccttcgcacg ggagactgca acattcagca
      661 tgctgttatc gctatcaata aacccgccgt cttcatgttt tgaatagact ggcgagaata
      721 tcacaatcgc tctttccgct ccctgaagag agtgcacggt gcccactgtg agcgactttt
      781 cattcgtgcc tgtactgata ccttgtttgc ccagcacctg tttgatagtg gatacctgag
      841 cgctaaaagg cgtcacaata ccgacaactt catgaagcga tttaccgtaa tgcgcttcaa
      901 tattttgctg gttctctgcc aaccagaccg ctatcgtttc agcctcaagc aaattatatc
      961 gacttccact acttgccagc tctcctttac catcaatatg gagatacccc attgcgggca
     1021 ttaaattgct ctcttcacgc cctcttttag gcaacaactt accgtgatag cagagcgtat
     1081 tacagtatcc aataatattg tcgtagcacc ggcggtgttc atataggtac ataccacgag
     1141 ccagttcggg atcatattga tagcgcgaag caaactgcgc tattttcata acgctgccag
     1201 atgcggcact tttaccaagg tctgcgattg ccgtatattt ctcggtaatc tcttcttgcg
     1261 tactgccaga cagaattttt tccgccagca tgttaccgac atcaatcgca ggagcaatac
     1321 tccatattgg cgggatctgc tccgtatcgc caatcactaa tgccttctta gctaatgcaa
     1381 acgaggcagc agccacttca ggaagcacct gcccggcttc atcgacaatg agtaaatcgg
     1441 caaaatcata caaataactt ttctcgaatt tacgttgtcc tttgtgctca cttatctgca
     1501 tattaccggg cagcatatag catgtcatta ccacacatgg cgtgagtttc attcgacgtt
     1561 gccaacgggc ggttacccct ttagcacctt ttttcttctt ctcgtcctgc agatcatcaa
     1621 tgctggccat atccatcagc caacgacctt cccagtagtg agtcgtcagt aaaaatgcag
     1681 ggaagcgaat ctgcgtatca gccagttcat cggcctgtga gaagctcagt tcctcgtcgc
     1741 cctcataccc taaatcaaat gccagcctct gccactcctg aaccgcctgc tgttctttaa
     1801 gaacgatttc atgggcggag tcaatctgct gccggtatgt tgtttgctcg cgctcagcgg
     1861 aattgagcag cccatcaata ttacgttcga tagtttcagg atcagaccac tgatttcctg
     1921 caatcagcgc gcctaattta tcttcgagaa acagctgtat ttggtactgt cgcttattgc
     1981 gaaccgccgg gagccaggaa aataatgaat agatcagtga ttcaccggcg cgatattttt
     2041 tccattccgt tttagcactc ttcagtagag tgactttttg ttcttgtccg gaaagtaatt
     2101 tatttaaatt atcgagatat tgctcaatat cattagcaat aagctcacgc gcagcccgaa
     2161 tctggcttaa cgtttgccat gttgcgttca gtcttatcag ttgctcagat tttgctgcca
     2221 actgaccatg caggagttca atgacctttt caggggatga acactctttt ccaggaaagg
     2281 ctgccttagc cttttccaga taaaacagca gtgcatcctc tacatactct tttgactcaa
     2341 cctggttgaa gaaatcttca gtttgatatt ttttggctgc ctcagcttta cgactgcttg
     2401 agggaaaata agcaccgaag cttttcagct ctggcaacca tcgcccggcc atcgcacctg
     2461 aaccttgcga aaagtctttc ccgaatgcct caataatgtt cgttacagcc tggttattcg
     2521 ttgaagtcgc gataataacc ggaggctcag atttttcgag agccgctctg gcccactgcg
     2581 tggcgatgat agaaagcacc agcgtggttt ttccggttcc cggagggcca ttaacagcaa
     2641 ggatatcgcc atgtcttgca tccagaaaat ggcttaaggc atcgcgctga gcctttgcca
     2701 gcggaaactt atctccggag tgtccaagcc tgtcgctgaa ttttgctcct ggggccagca
     2761 aagactctgc agcatgaacc tctcgcgagg cgaagcgatt gaagagcggc acatccttat
     2821 tgcaaacaat caggtgatca taaagagaaa ggatatggga actggcaccg ccagattgag
     2881 ccgttttaac aatataaccg tgctcagcga gttcatattg ctcaggtttt tcaatccagt
     2941 cgccggcaac gctcttcagt agcctctctg agtcatacag atattgacgc cactcctgct
     3001 gcaaggcggc atatcgtgct tcccgttctt catccgtttc ggcagtctta tcaacgctgt
     3061 catcaaagtt gatagagaac gtggtatggg tcgtcttgta tttgtcatac tgcccaatct
     3121 caccaatcga aaatgctcct tttggcaaag gttcaagcag gtctctggga atggaggtcg
     3181 ctggcgtcgg atataaaaaa ccctcacggc ttagcaaggc tggcgtcact atcggggtaa
     3241 caatatcagg cgcacctgca gaacggtcct taccatgctg cagtaaccgg aaataaactt
     3301 ttgggcgcaa gatgacatcg accgtttcga catcgtcttt ttctccctta aaaaatttac
     3361 tgacaatcgc ttcgtcaaga cgtcccgccg ctatcccatg ccagtgagtg aaatttttgg
     3421 cgtctttccg ttcaaaactg ccctttcctg actcagcatc cgcaagcgag ttgcgccagt
     3481 atgaggtaaa ccctaaagca ttttcatcca ttaccagtcc tgtgactcaa tgaaatccat
     3541 tgatttatcg aatataaaac caccatatgg acaactattg tccatattga aaaagttgga
     3601 tgcaatgttt tgtttataat tcggtatcaa atgtactggt tgtacaaggt tcagaacaca
     3661 agcacattca acagtacgtc aggactattg atttcataac agtgaataca ttttttaccg
     3721 tttataatga ctgcctgtgt tggacaagga taccagaaca gccgaaaaca ccgggggtta
     3781 ttacctgctc tcatggaaac cagatattcc tggtgctaat cacagtcata aatagcctga
     3841 agaacatgac gaagggactt taatggaagc cggagtaact tgccagaaca tcaatcttcg
     3901 tcatcattta c
//
LOCUS       DAZRUF010000296         3910 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZRUF010000296 DAZRUF010000000
VERSION     DAZRUF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.299"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            26..592
                     /locus_tag="ACHXOL_003920"
     CDS             26..592
                     /locus_tag="ACHXOL_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR6810766.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            596..1171
                     /locus_tag="ACHXOL_003921"
     CDS             596..1171
                     /locus_tag="ACHXOL_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR6810767.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1213..2265)
                     /locus_tag="ACHXOL_003922"
     CDS             complement(1213..2265)
                     /locus_tag="ACHXOL_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR6810768.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2490..3410
                     /gene="lpxL"
                     /locus_tag="ACHXOL_003923"
     CDS             2490..3410
                     /gene="lpxL"
                     /locus_tag="ACHXOL_003923"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR6810769.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3582..3767
                     /locus_tag="ACHXOL_003924"
                     /pseudo
     CDS             3582..3767
                     /locus_tag="ACHXOL_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          987 c          952 g         1000 t
ORIGIN      
        1 tcgcaaattc gtattggatg cacttatgtc attcacaaat acccctgaac gctatggtgt
       61 tatatctgcg gccttccatt ggctaagcgc aattattgtc tacggcatgt ttgctttagg
      121 tctgtggatg gtcacgctca gttattacga tggctggtat cacaaagcac ccgaactgca
      181 taaaagcatc ggtattttgt taatgatggg gctggttatt cgcgttctgt ggcgtgtcat
      241 atctccaccg ccgggtccgc tgccgagtta ttcgcccatg actcgtcttg ccgcaaaggc
      301 tggacatctc gcattgtacc tattgctgtt cgccatcggc atcagcggct atctgatctc
      361 aactgccgat ggtaaaccga tcagcgtttt tggctggttt gacgtccccg cgacccttgc
      421 cgacgctggc gcacaggcag actttgccgg tgccctgcat ttttggctcg cgtggagcgt
      481 cgtagtactg tccgttatgc acggatttat ggccctgaag catcatttca tcgataaaga
      541 cgacactctg aagcgcatgc tgggaaagtc gtcatctgac tatggagtat aaaaaatgaa
      601 aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc tctgccggtt cagcggttgc
      661 cgccgattac aaaattgaca aagaaggtca gcacgccttt gttaatttcc gcatccagca
      721 ccttggctat agctggttat acggcacctt taaagatttc gacggtactt ttacctttga
      781 cgaaaaaaat ccggctgccg ataaagtgaa tgtgacaatt aacaccacca gcgtcgatac
      841 taatcacgcc gaacgcgata aacatcttcg cagtgcagat ttcctcaata ccgcaaaata
      901 tccacaggca acattcacct ccaccagcgt gaagaaagac ggtgacgaac tggatattac
      961 cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg ctggaagcga aattaattgg
     1021 tcagggcgac gacccatggg gtggtaaacg tgcaggcttc gaggccgaag gcaaaattaa
     1081 gctcaaggac ttcaatatca agacagattt aggtccagct tctcaggaag tggatctgat
     1141 tatttcagtg gaaggcgtac agcagaagta agcgtgaaat gttgccggat ggcacgcatc
     1201 cggcaatgat atttattctg ttggatcagg aatgcccagt gttgtattca gacgtccacg
     1261 agacttatta aagatcttat tgccattttc acgtcctgcc cgacggcgtc gctgttcttc
     1321 tggcggtaac gcgctttctt cgcagcaaat ctcactacaa caacctttgt atttttccgc
     1381 gcatactgga cactgaataa acagcaggtg gcagccatca tttttacagt tggtatggct
     1441 gtcgcacggc gcgccgcact ggtggcaatg cgcgataatc tcatccgata tacgttcgcc
     1501 catccgctcg tcaaaaacaa aatttttgcc aataaaacgc accggcaagc cctgctcgcg
     1561 cgccttacgg gcgtattcaa taattccgcc ctcgatatgc cagactttgt tgaatccgtt
     1621 atgtttcatc caggcactgg ccttttcaca acgaatgccg ccggtgcagt acatgacgat
     1681 ttttttatct ttatgtgcct gcatcatctc gactgctttt ggcagctgct cacggaaggt
     1741 atctgccgga atttcgagcg cgttttcaaa gtgccccact tcatactcat agtggttacg
     1801 catgtcgata aatagtgcat cgggatcgtc aagcatggcg ttcacttccg ccgcttgcag
     1861 atactcacca acattgctgg catcaaagtg aggatcgtca ataccgtcgg caacaatgcg
     1921 atcgcgtacc ttcatgcgca gtacccagaa ggatttcccg tcatcatcca acgcgatatt
     1981 caggcgtaaa ccctctaaag ccgggtcgaa ggcatagagc tgcgcgcgaa atgtttcaac
     2041 attgctcgca ggtacgctga tttgcgcgtt aatgccctca tgcgccagat acactcgccc
     2101 aaaaacattc agcgcggtaa acagctgata taaagcgtca cgggtcgcct taggatcggc
     2161 gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt tcgctctcaa ccaacatttt
     2221 ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact ggcatggtgt acggttcctg
     2281 cgagatggga aagtaaaaat ccgcggcatg atatagcaat tatcgataat taacatccac
     2341 acattttacg ctacatttgc gcattaaaaa ttatttgtta tttacaagcg cggcaatttc
     2401 gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa atgcgacaat acatacaatt
     2461 gcccgaatag gttgaaaaac aggattgata tgacgaatct acccaagttc tccaccgcac
     2521 tgcttcatcc gcgttattgg ttaacctggt tgggtattgg cgtactttgg ttagtcgtgc
     2581 aattgcccta cccggttatc taccgcctcg gttgtggatt aggaaaactg gcgttacgtt
     2641 ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga actgtgcttc ccggaaatga
     2701 gcgaacaaga acgccgtaaa atggtggtga agaatttcga atccgttggc atgggactga
     2761 tggaaaccgg tatggcgtgg ttctggccgg accgccgaat cgcccgctgg acggaagtga
     2821 tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg cggcatcctg ttagttggca
     2881 tccattttct gacactggaa ctgggcgcgc ggcagtttgg tatgcaggaa ccggggattg
     2941 gcgtttaccg cccgaacgat aatccactga ttgactggct acaaacctgg ggccgtttgc
     3001 gctcaaataa atcgatgctc gaccgcaaag atttaaaagg catgattaaa gccctgaaaa
     3061 aaggcgaagt ggtctggtac gcaccggatc atgattacgg cccgcgctca agcgttttcg
     3121 tcccgttatt tgccgttgag caggctgcga ccacgaccgg aacctggatg ctggcacgga
     3181 tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa gccagatggc aaagggtatc
     3241 aattgattat gctgccgcca gagtgttctc cgccactgga tgatgccgaa actaccgccg
     3301 cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc accagagcag tatatgtggt
     3361 tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc acgctattaa atctcccctg
     3421 ccggatgctt cagaatggca tccggcatta ccacagcaaa tccccctgat ttagcgataa
     3481 aagctctctg gattgcgccc cctggaagtc gggcgcataa ttagtgtgct tatcttttct
     3541 tcttatgttc accgcgcctg gcgcaccaac agcggattgc tatgtcaccc tgtggaaatg
     3601 acacccctat aaactggaaa cgaaacctga tcgtcgcctg gctaggctgt tttcttaccg
     3661 gtgccgcctt cagtctggta atgcccttct tacccctcta cgttgagcag cttggcgtta
     3721 ccggtcactc cgccctgaat atgtggtccg gtattgtctt cagtataata ccttcctaaa
     3781 tacactacct gagagaagag atgaaaagag ctatcagcta catgaggttt tcaactgaga
     3841 agcagataaa aggggactct atacgaagac agtctaagct ggtcactgac tggttatcaa
     3901 agaatcctga
//
LOCUS       DAZRUF010000297         3877 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZRUF010000297 DAZRUF010000000
VERSION     DAZRUF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.300"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            70..1056
                     /gene="dcyD"
                     /locus_tag="ACHXOL_003925"
     CDS             70..1056
                     /gene="dcyD"
                     /locus_tag="ACHXOL_003925"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR6810770.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            1071..1739
                     /gene="tcyL"
                     /locus_tag="ACHXOL_003926"
     CDS             1071..1739
                     /gene="tcyL"
                     /locus_tag="ACHXOL_003926"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR6810771.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            1736..2488
                     /gene="tcyN"
                     /locus_tag="ACHXOL_003927"
     CDS             1736..2488
                     /gene="tcyN"
                     /locus_tag="ACHXOL_003927"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR6810772.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            2718..3440
                     /gene="sdiA"
                     /locus_tag="ACHXOL_003928"
     CDS             2718..3440
                     /gene="sdiA"
                     /locus_tag="ACHXOL_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR6810773.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(3507..3731)
                     /gene="yecF"
                     /locus_tag="ACHXOL_003929"
     CDS             complement(3507..3731)
                     /gene="yecF"
                     /locus_tag="ACHXOL_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR6810774.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(3718..>3877)
                     /gene="yecU"
                     /locus_tag="ACHXOL_003930"
     CDS             complement(3718..>3877)
                     /gene="yecU"
                     /locus_tag="ACHXOL_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR6810775.1"
                     /translation="GHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTANFHCNM
                     TRKINEYA"
BASE COUNT          949 a          911 c         1027 g          990 t
ORIGIN      
        1 gcctttttta tttacgcgtt ttcagcgtgc ataataagaa aatcacaaca acatacatcg
       61 gaggctgtta tgccactgca taatttaacc cgttttccac ggttggagtt tatcggcgcg
      121 ccaacgccgc tcgaatatct gccgcgcttt tctgattatc taggacggga aattttcatc
      181 aaacgggatg acgtcacccc catggcaatg ggcggcaata aattacgtaa gctagaattt
      241 ctcgcggcag atgctctgcg cgaaggtgcc gatacgctga ttactgccgg ggtgatccag
      301 tctaaccatg tgcggcagac tgccgcagtt gcggcgaaac tcggtctgca ctgtgtggcg
      361 ctgctggaaa atcctattgg cacaaccgca gaaaactatt taaccaacgg caatcgcttg
      421 ttgctggatc tgttcaatac ccagattgaa atgtgcgaag cactgaccga tcccaatgcc
      481 caactggaag agctggcgac gcgagtcgaa gcacaaggct ttcgcccgta tgtcattccg
      541 gttggcggtt ctaatgctct tggcgcgcta ggttatgtgg agagtgcgct ggaaatcgcg
      601 caacagtgtg aaggggcggt taatatttcg tcggtggtag tcgcatcggg cagtgccgga
      661 actcacgccg gactggctgt tgggctggaa cacctgatgc ctgaaagcga actgattggc
      721 gtgaccgtgt cgcgttccgt tgccgatcag ttgccgaaag tggttaacct acaacaggcg
      781 attgcgaaag aactggagct gaccgcatca gcggaaatta tactctggga tgactatttt
      841 gcacctggct acggcgtgcc gaacgacgaa ggcatggaag cagtgaaatt gctggcgcgg
      901 ctggaaggca ttctgcttga tcctgtgtat accggaaaag cgatggcggg gctgattggc
      961 tgtatcagtc agaaacgctt caaagatgaa gggccgattc tgtttattca taccggcggt
     1021 gcgcctgcgc tgttcgccta tcatccccac gtttagctcc gggaacgctc atgcaagaaa
     1081 gtatacaact ggttattgat tccttgccgt tcctgctgaa aggggctggg tatacgctgc
     1141 aactcagtat cggcggcatg ttttttggct tactgctcgg gtttattctc gcgctgatgc
     1201 gcctgtcgcc aatctggccc gtgcgctggc tggcgcgttt ttatatctct atttttcgtg
     1261 gtacaccgct catcgcacaa ctgtttatga tctattacgg cctgccgcag tttggtatcg
     1321 aattagatcc cattccgtca gcaatgattg gtctgtcgct gaacactgcc gcctatgccg
     1381 ccgaaacact gcgcgccgcc atttcttcta ttgataaagg tcagtgggaa gcagctgcca
     1441 gtattggtat gacgccatgg cagactatgc gtcgcgcgat tttgccacag gcggcgcggg
     1501 tggcgttgcc gccgctgtcg aacagcttta tcagcctggt aaaggatacg tcgctggccg
     1561 cgacaatcca ggtgccggag ctgttccgtc aggcgcagtt gattacctcg cgcacgctgg
     1621 aggtgttcac catgtacctg gcggcttcgc tcatctactg gatcatggcg acagtattat
     1681 cgacgttgca gaaccatttt gagaatcaac ttaatcgcca ggagagagaa ccaaaatgag
     1741 tgccattgaa gttaagaacc tggtgaaaaa atttcacggt cagacggtgc tgcacggtat
     1801 cgacctcgag gtaaagcctg gcgaagtggt ggcaattatc ggtccgagtg gttccggcaa
     1861 aaccacgttg ctacgcagca taaatctgct ggaacaaccg gaagcgggaa cgatcaccgt
     1921 tggcgatatc actattgata ctgcacgttc attaagtcag caaaaatcgc tgatccgcca
     1981 gttgcgtcag cacgtcgggt ttgtcttcca gaactttaat ttgtttccgc atcgtacggt
     2041 gctggaaaac attattgaag ggccggtgat cgtcaaaggc gaaccgaaag aagaggccac
     2101 ggcgcgcgct cgcgagctgc tggcgaaagt tgggctggcc ggtaaagaaa ccagctatcc
     2161 acgtcgtttg tctggcggtc aacagcagcg tgtagcgatt gcgcgtgcgc tggcaatgcg
     2221 tcctgaggtg attttgtttg acgagccaac gtcagcgctg gatccagagc tggtgggcga
     2281 agtcctgaac acaatccgcc agctggcgca ggaaaagcgc acgatggtga ttgtgacgca
     2341 cgaaatgagc tttgcccggg atgttgcgga ccgggcgatt tttatggacc aggggcggat
     2401 agtcgagcag ggggcagcaa aagcgttatt tgctgatccc cagcagcctc gcacccgcca
     2461 gttcctcgag aagtttctgc tgcaataata agaaaaaatc agccccgacg attcacctgt
     2521 cggggcggga cgccatttca agcgtgataa aactgcttaa caaatcagca taactcatta
     2581 ataacataag agaatgcgat ggcttgcaaa agtaattcat tgcctgaata atataaatta
     2641 tatataaatc ttatttatgt gatagtttga attatcatta taaatgatac tcactctcag
     2701 gggcgttgcg gtttactatg caggatacgg attttttcag ctggcgtcgc acgatgctgt
     2761 tgcgttttca gaggatggag gccgcagaag aggtctacca tgaaatagag cttcaggctc
     2821 agcagctgga gtacgattac tattcgttat gtgttcgcca cccggtacca ttcacgcgac
     2881 ctaaagtggc tttctacacc aattaccctg agtcgtgggt tagttattat caggcaaaaa
     2941 attttctcgc aattgatcct gtgctgaacc ctgaaaactt tagtcagggc catttaatgt
     3001 ggaatgatga cttattcagc gaagcacagc cattatggga agccgcgcac gcacatggtt
     3061 tacgccgcgg tgtcactcag tatttaatgc tgccaaatcg ggcgctgggc tttttgtcct
     3121 tttcccgttg cagcacgcgc gaaataccca ttcttagtga tgaactgcaa ttaaaaatgc
     3181 agttactggt gcgcgaaagt ctgatggctc tgatgcgttt aaatgatgaa atagtgatga
     3241 cgccagagat gaatttcagc aagcgcgaaa aagaaattct gaaatggacg gcggaaggga
     3301 agacatcagc agagatagcg atgattttgt caatctctga gaatacggtc aatttccatc
     3361 agaaaaacat gcagaaaaaa atcaatgcac caaataagac ccaggtggcc tgttacgcgg
     3421 ccgctactgg cttaatttga tcgcttttct ttctgcgtgc cagatgcaaa aatcggctga
     3481 aaggcacgct ctcagccggt tttatattac tgacggtagg cttgtttaat ttgcttaacc
     3541 gttttggaaa atactgcagc ctgggtttcg tcttcgatca gcgcaagctg tttttccatc
     3601 tttaacatca cgcggcccgc gtcagcttgt cccatcgcct gtagcatcag cgtcagcatc
     3661 gctttcaggc aggagacttc atttgccagt tcttgattat tctcggcagt agaaaaatca
     3721 ggcgtactca tttattttcc tcgtcatgtt gcaatgaaaa tttgcggtga aaaatgttaa
     3781 ggcggcggag tataccataa gctttgctaa aaatagcagt ggttgttttt tgagcgtgac
     3841 gtcaacaatg ctattaaatg cgttaatata atgacca
//
LOCUS       DAZRUF010000298         3873 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZRUF010000298 DAZRUF010000000
VERSION     DAZRUF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.301"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            239..2155
                     /gene="dnaK"
                     /locus_tag="ACHXOL_003931"
     CDS             239..2155
                     /gene="dnaK"
                     /locus_tag="ACHXOL_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR6810776.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2244..3374
                     /gene="dnaJ"
                     /locus_tag="ACHXOL_003932"
     CDS             2244..3374
                     /gene="dnaJ"
                     /locus_tag="ACHXOL_003932"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR6810777.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3478..3630)
                     /gene="mokC"
                     /locus_tag="ACHXOL_003933"
     CDS             complement(3478..3630)
                     /gene="mokC"
                     /locus_tag="ACHXOL_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR6810778.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1024 a          958 c         1037 g          854 t
ORIGIN      
        1 atgaataaaa ttgctgtcaa ttttacgtct tgtcctgcca tatcgcgaaa tttctgcgca
       61 aaagcacaaa aaatttttgc atctccccct tgatgacgtg ggttacgacc ccatttagta
      121 gtcaaccgca gtgagtgagt ctgcaaaaaa atgaaattgg gcagttgaaa ccagacgttt
      181 cgcccctatt acagactcac aaccacatga tgaccgaata tatagtggag acgtttagat
      241 gggtaaaata attggtatcg acctgggtac taccaactct tgtgtagcga ttatggatgg
      301 caccactcct cgcgtgctgg agaacgccga aggcgatcgc accacgcctt ctattattgc
      361 ctatacccag gatggtgaaa ctctggttgg tcagccggct aaacgtcagg cagtgacgaa
      421 cccgcaaaac accctgtttg cgattaaacg cctgattggc cgccgcttcc aggacgaaga
      481 agtacagcgt gatgtttcca tcatgccgtt caaaattatt gctgctgata acggcgacgc
      541 atgggtcgaa gttaaaggcc agaaaatggc accgccgcag atttctgctg aagtgctgaa
      601 aaaaatgaag aaaaccgctg aagattactt gggtgaaccg gtaactgaag ctgttatcac
      661 cgtaccggca tactttaacg atgctcagcg tcaggcaacc aaagacgcag gccgtatcgc
      721 tggtctggaa gtaaaacgta tcatcaacga accgaccgca gctgcgctgg cttacggtct
      781 ggacaaaggt actggcaacc gtactatcgc ggtttatgac ctgggtggtg gtactttcga
      841 tatttctatt atcgaaatcg acgaagttga cggcgaaaaa accttcgaag ttctggctac
      901 caacggtgat acccacctgg ggggtgaaga cttcgacagc cgtctgatca actacctggt
      961 tgaagaattc aagaaagatc agggcattga cctgcgcaac gatccgctgg caatgcagcg
     1021 cctgaaagaa gcggcagaaa aagcgaaaat cgaactgtct tccgctcagc agaccgacgt
     1081 taacctgccg tacatcactg cagatgcgac cggtccgaaa cacatgaaca tcaaagtgac
     1141 tcgtgcgaaa ctggaaagcc tggttgaaga tctggtaaac cgttccattg agccgctgaa
     1201 agttgcactg caggacgctg gcctgtccgt atctgatatc gacgacgtta tcctcgtggg
     1261 tggtcagact cgtatgccaa tggttcagaa gaaagttgct gagttctttg gtaaagagcc
     1321 gcgtaaagac gttaacccgg acgaagctgt agcaatcggt gctgctgttc agggtggtgt
     1381 tctgactggt gacgtaaaag acgtactgct gctggacgtt accccgctgt ctctgggtat
     1441 cgaaaccatg ggcggtgtga tgacgacgct gatcgcgaaa aataccacta tcccgaccaa
     1501 gcacagccag gtgttctcta ccgctgaaga caaccagtct gcggtaacca tccatgtgct
     1561 gcagggtgaa cgtaaacgtg cagctgataa caaatctctg ggtcagttca acctggatgg
     1621 tatcaacccg gcaccgcgcg gcatgccgca gatcgaagtt accttcgata tcgatgctga
     1681 cggtatcttg cacgtttccg cgaaagataa aaacagcggt aaagagcaga agatcaccat
     1741 caaggcttct tctggtctga acgaagatga aatccagaaa atggtacgcg acgcagaagc
     1801 taacgccgaa gctgaccgta agtttgaaga gctggtacag actcgcaacc agggcgacca
     1861 tctgctgcac agcacccgta agcaggttga agaagcaggc gacaaactgc cggctgacga
     1921 caaaactgct atcgagtctg cgctgactgc actggaaact gctctgaaag gtgaagacaa
     1981 agccgctatc gaagcgaaaa tgcaggaact ggcacaggtt tcccagaaac tgatggaaat
     2041 cgcccagcag caacatgccc agcagcagac tgccggtgct gatgcttctg caaacaacgc
     2101 gaaagatgac gatgttgtcg acgctgaatt tgaagaagtc aaagacaaaa aataatcgcc
     2161 ctataaacgg gtaattatac tgacacgggc gaaggggaat ttcctctccg cccgtgcatt
     2221 catctagggg caatttaaaa aagatggcta agcaagatta ttacgagatt ttaggcgttt
     2281 ccaaaacagc ggaagagcgt gaaatcaaaa aggcctacaa acgcctggcc atgaaatacc
     2341 acccggaccg taaccagggt gacaaagagg ccgaggcgaa atttaaagag atcaaggaag
     2401 cttatgaagt tctgaccgac tcgcaaaaac gtgcggcata cgatcagtat ggtcatgctg
     2461 cgtttgagca aggtggcatg ggcggcggcg gttttggcgg cggcgcagac ttcagcgata
     2521 tttttggtga cgttttcggc gatatttttg gcggcggacg tggtcgtcaa cgtgcggcgc
     2581 gcggtgctga tttacgctat aacatggagc tcaccctcga agaagctgta cgtggcgtga
     2641 ccaaagagat ccgcattccg actctggaag agtgtgacgt ttgccacggt agcggtgcaa
     2701 aaccaggtac acagccgcag acctgtccga cctgtcatgg ttctggtcag gtgcagatgc
     2761 gccagggatt cttcgctgta cagcagacct gtccacactg tcagggccgc ggtacgctga
     2821 tcaaagatcc gtgcaacaaa tgtcatggtc atggtcgtgt tgagcgcagc aaaacgctgt
     2881 ccgttaaaat cccggcaggg gtggacactg gagaccgcat ccgtcttgcg ggcgaaggtg
     2941 aagcgggcga gcatggcgca ccggcaggcg atctgtacgt tcaggttcag gttaaacagc
     3001 acccgatttt cgagcgtgaa ggcaacaacc tctattgcga agtcccgatc aacttcgcta
     3061 tggcggcgct gggtggcgaa atcgaagtac cgacccttga tggtcgcgtc aaactgaaag
     3121 tgcctggcga aacccagacc ggtaagcttt tccgtatgcg cggtaaaggc gtcaagtctg
     3181 tccgcggtgg cgcacagggt gatttgctgt gccgcgttgt cgtcgaaaca ccggtaggcc
     3241 tgaacgagaa gcagaaacag ttgctgcaag agctgcaaga aagcttcggt ggcccaaccg
     3301 gtgagcacaa cagcccgcgc tcaaagagct tctttgatgg tgtgaagaag ttttttgacg
     3361 acctgacccg ctaacctccc caaaagcctg cccgtgggca ggcttgggta aaattagggt
     3421 gcgttgaaga tatgcgagca cctgaaaagt ggcggggatc actccccgcc gttgctctta
     3481 ctcggattcg taagccgtga aaacagcaac ctccgtctgg ccagttcgga tgtgaacctc
     3541 acagaggtct tttctcgtta ccagcgccgc cactacggcg gtgatacaga tgacgatcag
     3601 ggcgacaatc atcgccttat gctgcttcat tgctctcttc tccttgacct tacggtcagt
     3661 aagaggcact ctacatgtgt tcagcatata gggggcctcg ggttgatagt aaaatatcac
     3721 tcggggcttt tctctatctg ccgttcagct aatgcctgag acagatagcc tcaagcaccc
     3781 gccgctatta tatcgctctc tttaacccat tctgttttat cgattctaat cctgaagacg
     3841 cctcgcattt ttgtggcgta attttttaat gat
//
LOCUS       DAZRUF010000299         3827 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZRUF010000299 DAZRUF010000000
VERSION     DAZRUF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.302"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..275)
                     /gene="chbB"
                     /locus_tag="ACHXOL_003934"
     CDS             complement(<1..275)
                     /gene="chbB"
                     /locus_tag="ACHXOL_003934"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR6810779.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGV"
     gene            complement(574..912)
                     /gene="osmE"
                     /locus_tag="ACHXOL_003935"
     CDS             complement(574..912)
                     /gene="osmE"
                     /locus_tag="ACHXOL_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR6810780.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1114..1941
                     /gene="nadE"
                     /locus_tag="ACHXOL_003936"
     CDS             1114..1941
                     /gene="nadE"
                     /locus_tag="ACHXOL_003936"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR6810781.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREADIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2171..3058
                     /gene="cho"
                     /locus_tag="ACHXOL_003937"
     CDS             2171..3058
                     /gene="cho"
                     /locus_tag="ACHXOL_003937"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR6810782.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3018..3593)
                     /gene="ves"
                     /locus_tag="ACHXOL_003938"
     CDS             complement(3018..3593)
                     /gene="ves"
                     /locus_tag="ACHXOL_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR6810783.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGGVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT          977 a          949 c          914 g          987 t
ORIGIN      
        1 acgcctaaac catcgacttt gccataaagc agcgagtcaa ttacttcaac cggtttgttg
       61 ggtaacaaac gctggatttc gggcaacata taagcaatct gcggccctaa taacacgaca
      121 tcggcattct gacctttttc accagccagt gtttccggaa atgcttcaat aatgaccgga
      181 acttcatatt tttctgcctg tgcgcgcatt tttgatacca gtaaagaggt agacatgccc
      241 gcagaacaaa acagataaat gtgtttcttt tccataaaac tgccctcatc gacgattatc
      301 tgtcagccag acactccgca agccttaacc tgcttccatg ctctgggtaa cttgcgaaac
      361 caacatgact ttttttgtgg ctgaaaggag tatacggtat agacctgagc aatagtattt
      421 ctttgacctt ataaattgtt tctccttgac ctgtggttat gaccctcttc acatttcggg
      481 caaataattc gcgaagataa ttaagaactc aggccaaaaa aaaccggcgc aatggccggt
      541 ttccgttgtt actcaagctt tcagacgaat tgattacttc gcagcctgtg gatcagtgtc
      601 gtattcagca caggtctgat aaccggagtt gatgacatgt ccggtatcat ctaacgcgac
      661 aaagtaggtt tctgctttac catcacgttg acccaggatg taggtctggc aagtaccacg
      721 agcatggatc atgctcactt cagacgaagg tttacccgca atttgtgcaa cctgcgcccg
      781 gctcatgcct tttttgacgt ctttcaccac aggctgtaca aactggtctt tagtacgatc
      841 ataagccgta caacccgcca gcatggttaa taccgccgct gcactcagaa ttcctgccat
      901 attcttgttc atattccgtc ctcttgttta tcagcgtgtt agataagcct ggaatacatt
      961 gggcgctttt tcaagcccgc gaacgaaacg gctccgcttt cagaggattc ctgtatgacg
     1021 ttttaaccac cattcagccc gctgtcgctt gtcgtttcag tggcaacggg ttagctttaa
     1081 ggaagttttg tcttttctgt ctggaggggt tcaatgacat tgcaacaaca aataataaag
     1141 gcgctgggcg caaaaccgca gattaatgct gaagaggaaa ttcgtcgtag tatcgatttt
     1201 ctgaaaagct acctgcaaac ttatccgttc attaaatcac tggtgctcgg gatcagcggc
     1261 ggtcaggact ccacgcttgc cggaaagctg tgccagatgg cgattaatga gctgcgcctg
     1321 gaaaccggca acgaatcact gcaatttatt gccgtacgcc tgccctatgg tgttcaggcc
     1381 gacgaacaag attgccagga tgccattgcc tttattcaac cggatcgcgt attaaccgtt
     1441 aatatcaagg gcgcggtatt ggccagcgaa caggcattgc gggaagcaga cattgaactg
     1501 agcgattttg tccgtggcaa tgaaaaagcg cgtgagcgga tgaaagcaca atatagcatt
     1561 gcgggtatga ccagcggtgt cgtggtgggc accgatcatg cagcagaagc cattaccgga
     1621 ttcttcacta aatatggtga cggcggtaca gacattaatc cgctgtatcg tctcaacaaa
     1681 cgtcagggta aacagttact ggcggcatta ggttgcccgg aacaccttta taagaaagcg
     1741 ccaacggccg atctggaaga tgatcgccct tctctgccag atgaagtggc actcggcgtg
     1801 acctatgaca atatcgacga ctatctggaa gggaaaaacg tacctgaaca ggtcgccaga
     1861 acaatagaga actggtatct gaaaaccgaa cataaacgcc gtccgccaat taccgttttc
     1921 gatgatttct ggaaaaagta ataatttgca cataatggtt tgtgcgaaaa agagtaattt
     1981 gttcacgccg gatgcggcgt gaacgcctta ttcgacctac aaaactatgc aaattcaata
     2041 tattgcagga gtcgaatagg cctgacaggc gtagcacgtc agacggtgta acctttgtca
     2101 tcgacccgcc tcttttttaa tcgctccccg cctgttacac tggatagata accagcattc
     2161 ggagtcaaca gtggtacggc gtttaacttc tccgcggctc gaatttgaag ctgcggcaat
     2221 ttatgaatat cccgaacatt tacgttcatt ccttaatgac ttacccaccc gaccaggggt
     2281 gtatctgttt catggcgaaa gtgacaccat gccgctctat atcggcaaaa gcgttaatat
     2341 ccgcagtcgg gtgctttctc atttacgcac cccggatgaa gccgccatgc tacggcaatc
     2401 ccggcgaatt agctggatct gtaccgcagg cgaaatcggc gctctgctcc ttgaagcgcg
     2461 attaatcaaa gaacaacagc cgctgtttaa taaacggttg cgccgcaatc gccagctctg
     2521 tgccctgcaa ttaaatgaaa agcgcgtcga tgtggtgtat gccaaagagg tggatttttc
     2581 acgagccccc aacctgtttg gcctgtttgc caataggcgc gcagctttgc aagcattgca
     2641 gaccatcgct gatgaacaaa aactttgtta tggcctgctg ggactggaac cgttaagtcg
     2701 cggtcgtgca tgttttcgtt cagcgctaaa acgttgcgcc ggagcatgct gcggtaaaga
     2761 gagccatgag gaacatgcgc tacgcttgcg ccagtctctg gagcgtttgc gggtggtgtg
     2821 ttggccttgg caaggtgcgg tggcactgaa agaacagcac ccggaaatga ctcaatatca
     2881 tattattcaa aactggctgt ggctgggggc ggttaattcg ctggaagaag cgacaacgtt
     2941 aattcggaca cccgccgggt ttgatcacga cggttataaa attctttgta agccgctgct
     3001 ttccggtaac tatgaaatta ctgaacttga tccggcgaat gaccagcgag ccagttgatt
     3061 tcactgaaca acagtttacc ttgcggttgt aaaagccgca gggtgtgcct gccatcgaac
     3121 cagcacgcgc cctggtcggt agtgagcaat ttatcgccca gttgccacgc cccgttaatc
     3181 acaaacaccc ccccgccgcg tgagccaaag gtagtaaacg tacgttcggc aattctgact
     3241 ttcgccttac agacatccag gcgtgtcatg atgttgaaat ccatcgacat ctgcccttcc
     3301 gtcagtttcg cttttaccac ctggtccgct gcaaaggcaa aaggctgtaa cggttttaag
     3361 gtatggttaa agcggtctgc gctttcaagg agcatctcgc cgccttccag caacgtcact
     3421 atcctttcca tgccgggaaa caaagaaaac tccccattcg ccgcgatgga tgcaatgctg
     3481 gcacgccagt aaaaatcacg ttttgccggt gggaaagtgc aaatttcgcg cgtttcaccg
     3541 gcagcatttc gccacagatt caccgacatt ttacgcatat caaagtattc catactcgct
     3601 cccggcccgt tcttttcatt gttatcgttc aggtaaccgt ggatggttat cggcaactcg
     3661 ggtgatttac gttagtggtg atcaggcttt ttttacaact gtttgagaag agaaaagaaa
     3721 accgccgatc ctgtccaccg cattactgca aggtagtgga caagaccggc ggtcttaagt
     3781 tttttggctg aaagattatt cagcagttgc aggcatttta ccttttg
//
LOCUS       DAZRUF010000300         3827 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZRUF010000300 DAZRUF010000000
VERSION     DAZRUF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.303"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHXOL_003939"
     CDS             <1..196
                     /locus_tag="ACHXOL_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810784.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="ACHXOL_003940"
     CDS             207..374
                     /locus_tag="ACHXOL_003940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810785.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            371..715
                     /locus_tag="ACHXOL_003941"
     CDS             371..715
                     /locus_tag="ACHXOL_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810786.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1007..1162
                     /gene="hokD"
                     /locus_tag="ACHXOL_003942"
     CDS             1007..1162
                     /gene="hokD"
                     /locus_tag="ACHXOL_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR6810787.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1383..1671
                     /locus_tag="ACHXOL_003943"
                     /pseudo
     CDS             1383..1671
                     /locus_tag="ACHXOL_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1710..1988
                     /locus_tag="ACHXOL_003944"
     CDS             1710..1988
                     /locus_tag="ACHXOL_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810788.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1990..3048
                     /locus_tag="ACHXOL_003945"
     CDS             1990..3048
                     /locus_tag="ACHXOL_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR6810789.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            3049..3429
                     /locus_tag="ACHXOL_003946"
     CDS             3049..3429
                     /locus_tag="ACHXOL_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR6810790.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3426..>3827
                     /locus_tag="ACHXOL_003947"
     CDS             3426..>3827
                     /locus_tag="ACHXOL_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR6810791.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT         1034 a          852 c         1046 g          895 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgagaaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc gtattaaatg tttacagtct caacaaagga
     1501 agagtggagc tgcgttaatt cattttgtgg atgggcttgt gacggatggt tttatttcgc
     1561 gtgcaaatga atttgtcaca tcgttgccgt ctctgaaaga agctgggatt aaggctggtt
     1621 tttctgcttt tgaagatgag tgaattcatc tacaattcag cgcagggctg aacccctgtt
     1681 gagtaacact gtgccaccgg agaaagccga tggcgcaaaa ttccagacta cacaattctg
     1741 ataattcagc cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt
     1801 ggtaccagca tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca
     1861 ggcgcggatg cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg
     1921 tcaggctccc ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct
     1981 ggaggtaatg tgcgggtatt acttcgacct gttctggtac cggaactcgg tctggttatc
     2041 gttaagccag gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg
     2101 gaaccaaaaa gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg
     2161 gtggaagaca aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt
     2221 ggtgctggtg cattgtctga ctggctgttg cgccatatta aatcctgcca gtggccacac
     2281 ggcgattatc atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg
     2341 tgctggcact gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct
     2401 catcaaaacc tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag
     2461 gagcgtgaat tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac
     2521 gcgctaccgg aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct
     2581 gtgtacagtg aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag
     2641 cgcacaaaaa atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa
     2701 aagacggtgg tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag
     2761 aaatcacaac gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg
     2821 acgtgtggca atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg
     2881 ggaacaaagg ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta
     2941 catgccggag taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt
     3001 tttttaatgc acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc
     3061 atagaatttg ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc
     3121 acatattttg tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc
     3181 cagcagcggc tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca
     3241 ccggataagc gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg
     3301 catgcggggt tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag
     3361 ccagtatctg gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc
     3421 aaaaaatgaa actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg
     3481 atgcatcggc ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg
     3541 gcatgacaca gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta
     3601 gtatgaatga cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg
     3661 atcgcgtggc ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta
     3721 tggcttcgta tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct
     3781 gctgtcacgg aaaaaaattt attgaaagcg aggtttttac aaacaag
//
LOCUS       DAZRUF010000301         3805 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZRUF010000301 DAZRUF010000000
VERSION     DAZRUF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.304"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHXOL_003948"
     CDS             <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHXOL_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR6810792.1"
                     /translation="GVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSS
                     QASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQ
                     YINSPYLLMIAAYFVACLMSLAVTSATGLGVLLMATLFPVMVNVGISRGAAAAICASP
                     AAIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEH
                     ISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILE
                     FLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSF
                     GSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQAS
                     NLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVT
                     GK"
     gene            complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHXOL_003949"
                     /gene_synonym="citB"
     CDS             complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHXOL_003949"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR6810793.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHXOL_003950"
                     /pseudo
     CDS             complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHXOL_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
BASE COUNT          881 a          987 c          916 g         1021 t
ORIGIN      
        1 ggggttgtgg ttattgtggg tgtagctcgc tacatcatta aagggtattc cgccactggt
       61 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
      121 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
      181 atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggattt
      241 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
      301 ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt cgcctgtctg
      361 atgtctctgg ccgtcacttc cgcaaccggt ctgggtgttt tgctgatggc aaccctattt
      421 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg
      481 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca agcttccgaa
      541 gtgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc
      601 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
      661 catgaaatgt tagatgtcag tgaaatcacc accactgctc ctgcgtttta tgccattttg
      721 ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac
      781 atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc
      841 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg
      901 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
      961 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     1021 ctgatgctgg tattggtgat tctgacaatg ctggcggcag tcacgaccgg ttcaggcaat
     1081 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac
     1141 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     1201 gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta
     1261 aaacgcacct cggtgccggt gcttgttggt ttggtgattg ttatcgttgc tacagagctg
     1321 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     1381 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc
     1441 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     1501 cagtggtgca gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     1561 gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggtagct
     1621 cgtccttagg ttcaccgcga gcataagcat taaacatctc atcaatttgc ttctggctgg
     1681 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     1741 gccgttcata ggcaatgggc ttaatgagat aatcaaatac accacaacgt acggcttcag
     1801 acaccgtttc catatcgctg gctgcagtgg taaacaccac gtcgccggga taatgcgcct
     1861 gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta tcgagcaaga
     1921 ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccagattt cccgccagta
     1981 atatctgact gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg
     2041 tttcgtcctc aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata
     2101 tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag
     2161 cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca
     2221 cgcgtactga ccccctgctc aaatattttg tctcttagag actctggaac gccgcagccc
     2281 tgatcggcga cttcaatcac cacatcatcg ccttcatcgc tgaggaataa ttcaacgatc
     2341 ttgtttcctt catcgctacg caggctggct tcgaaggcgt tatcaagtaa attgccaaca
     2401 atggcggcaa actcggtgct atccagtcct ggcggcagtt gcgaaagctg gctaccgggg
     2461 acgatgatca tttttagccc cagttcgcgg gcgcgctgca ctttaccaaa aagcagcccc
     2521 gccacctggc gatcggcaaa cgcctcacgc aggctgtcaa taagctgttg ctgggcctga
     2581 gactccccct gcaccatggc cagcacgcga tcatactctt tcatctgcaa cagaccattg
     2641 agcgtcgaca tccaattgag atgctcgtgt cacaatgtac gaaggctctc gacgtattgt
     2701 tttatttgcg tcagttgcgc attgagggtg gaaatttcgt ctttactacg aaagctgata
     2761 atggccccca gcaaatcatc acctgaacga atagcttccc ggttggcaat aacgttcaga
     2821 ccattaaagt tcgccaccac atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg
     2881 gcgggcctga ccacttcagc aatgggttta cccaaccatt gccgtccggg agagctcagc
     2941 cccagcatct ttcttgcgtt acgattgatg gctgtaatgt aaccatgcgg atccaccgca
     3001 atcagccctt cataaaccga actaaacagc gcctcttgct gacggaccac gcgtgcgatt
     3061 tgctttggct ccatgcccat catttgtcga cggatatgcg cggccaggaa ccacgacagc
     3121 aacatcagaa tccctaacag cacgacaaac acgcctgcca tcggtaataa aaactcagcc
     3181 cgccagctat cgattttact caccaggtag ccaatcgaca ccacgccgat gacttttcca
     3241 tcgtcatcaa agattggcgt tttggcgcgc atcgccatcc ccattgcccc tttcccggta
     3301 atgaagtagc tctccccttt ctccagcgcg ccctgtttgg tgaactgcat aggataacca
     3361 attttctccg gattaagatg gtaaaggcgg atcgagtgcc ggtcgccaat caccacataa
     3421 tcaaaatcgg tatctctttg taatttgtta gcgatggtcg ccagccgttt gtagtcacgc
     3481 gttttcaccg cagagatgac actgtcattg gaggcaataa ttttcgcctg attcattgcc
     3541 atgtcgcgta catgaagcgt taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg
     3601 gctgcaatga caaatattga gaacaccaga atcagcaaaa agatacgcag cggaaatgcc
     3661 agtctttgga aaaatgcaaa ctgtttattc tcgttaagct gcaacattgt tttccttgcg
     3721 ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt gaatcaaaat acatagatat
     3781 taattatgat cgtggcaaat tttat
//
LOCUS       DAZRUF010000302         3798 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZRUF010000302 DAZRUF010000000
VERSION     DAZRUF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.305"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..82
                     /locus_tag="ACHXOL_003951"
     CDS             <1..82
                     /locus_tag="ACHXOL_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810794.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(99..908)
                     /gene="proC"
                     /locus_tag="ACHXOL_003952"
     CDS             complement(99..908)
                     /gene="proC"
                     /locus_tag="ACHXOL_003952"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR6810795.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1028..1486
                     /locus_tag="ACHXOL_003953"
     CDS             1028..1486
                     /locus_tag="ACHXOL_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR6810796.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1669..2193
                     /gene="aroL"
                     /locus_tag="ACHXOL_003954"
     CDS             1669..2193
                     /gene="aroL"
                     /locus_tag="ACHXOL_003954"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR6810797.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2243..2434
                     /gene="yaiA"
                     /locus_tag="ACHXOL_003955"
     CDS             2243..2434
                     /gene="yaiA"
                     /locus_tag="ACHXOL_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR6810798.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2692..3369
                     /locus_tag="ACHXOL_003956"
     CDS             2692..3369
                     /locus_tag="ACHXOL_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR6810799.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3441..3725
                     /gene="ppnP"
                     /locus_tag="ACHXOL_003957"
     CDS             3441..3725
                     /gene="ppnP"
                     /locus_tag="ACHXOL_003957"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR6810800.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          903 c          990 g          954 t
ORIGIN      
        1 tcgtgagtgg ttgaaatcgg cagatttggc gctttacaaa gcaaagaaag ccggacgtaa
       61 ccgcaccgaa gtggcggcct gacgtccggc gaaagtcatc aggatttgct gagtttttct
      121 gatttttcca tacacttcgt catcgcttcg atcactgcgg cacggaagcc tttctcttcc
      181 agtacgcgta ccgcttcaat ggtggtgcct cccggtgagc agaccatatc tttcagtgcc
      241 cccggatgtt ctcccgtttc cagcaccatt tttgcggaac ccattaccgc ctgagcggca
      301 aatttatacg cctgggcgcg tggcatcccg cccagcacgg cggcgtcggc catcgcttcg
      361 ataaacataa atacgtaggc tggcgaagaa ccgctcacac cgaccaccgg gtggatcatc
      421 ggctcagcaa ttacttccgc ttcgccaaag cagcggaaaa tattcagcac atcagcggta
      481 tcttcggggg ttaccagcgc gtttggcgtt acggaggtca tcccggcatt aaccagcgcg
      541 ggagtgttcg gcatggcgcg gataattttc cggtcatggc ccagcgcgcg ggcaagctgg
      601 tcgagcgtga cacctgcagc aatagaaacg accagagagt ctttattcag gctggaggtg
      661 atttcgctaa gcactttaat catgatgcca ggtttaacgg cggcaaaaat gatgtcggcg
      721 atttgcgcca cttcttgcgc cgattctgcg gcgttgatgc cgaactggtc atgcagggcg
      781 gcgactttat ccggggaggg ggtgtatacc cagatttgcc ctggaagcac ctgaccgctg
      841 gcaatcagac cgccgagaat ggcttttccc atattgccgc agccaataaa accgattttc
      901 ttttccattg cctcactcct gccgtgaaat tcattgtttt gataatcgct ggcagaagca
      961 taaacagaac tatgccggaa ggcaaaagcg cgacacaata gaggattacc caacaaagga
     1021 tgactttatg acaatttggg tggatgccga cgcgtgtccc aatgtaatta aagagatttt
     1081 gtatcgcgcg gcggaacgta tgcagatgcc gctggtactg gtagcaaacc agagtttacg
     1141 cgtgccgcca tcgcgattta ttcgtacgct gcgcgtcgcg gcaggtttcg acgttgccga
     1201 taacgaaatt gtccggcagt gtgaagcggg cgatttggtg atcaccgcag atataccttt
     1261 ggctgctgaa gccatcgaga aaggcgcagc ggcgcttaat ccgcgcggcg aacgttacac
     1321 gccagcgacc attcgtgagc gcctgacgat gcgcgatttt atggatacct tacgtgccag
     1381 tgggatccag accggcggac cagatagcct ttcacaacgt gaccgccagg cctttgccgc
     1441 ggagctggag aagtggtggc tggaagtgca acgtagtcgt ggctaaatgt aatttattat
     1501 ttacacttca ttcttgaata tttattggta tagtaaggga tgtattgaga ttttcacttt
     1561 aagtggaatt ttttctttac aatcgaaatt gtactagttt gatggtatga tcgctattct
     1621 catgacaccg gctttcgccg cattgcgacc tattggggaa aacccacgat gacacaacct
     1681 ctttttctga tcgggcctcg gggctgtggt aaaacaacgg tcggaatggc ccttgccgat
     1741 tcgcttaacc gtcggtttgt cgataccgat cagtggttgc aatcacagct caatatgacg
     1801 gtcgcggaga tcgtcgaaag ggaagagtgg gcgggatttc gcgccagaga aacggcggcg
     1861 ctggaagcgg taactgcgcc atccaccgtt atcgctacag gcggcggcat tattctgacg
     1921 gaatttaatc gtcacttcat gcaaaataac gggatcgtgg tttatttgtg tgcgccagta
     1981 tcagtcctgg ttaaccgact gcaagctgca ccggaagaag atttacggcc aaccttaacg
     2041 ggaaaaccgc tgagcgaaga agttcaggaa gtgctggaag aacgcgatgc gctatatcgc
     2101 gaagttgcgc atattatcat cgacgcaaca aacgaaccca gccaggtgat ttctgaaatt
     2161 cgtagcgccc tggcacagac gatcaattgt tgattttaga gcgcctatac ttaacgttca
     2221 tcccgtgaaa taaggaagaa cgatgccaac gaaaccgcct tatcctcgtg aagcatatat
     2281 agtgacgatt gaaaaaggaa agccaggaca gacggtaacc tggtaccaac tcagagccga
     2341 tcatcctaaa ccagactcgt tgatcagtga acatccgacg gctcaggaag cgatggatgc
     2401 gaaaaaacgc tatgaggacc ctgacaaaga gtgaccgcat cagactgctc ggaagggatt
     2461 ctgagtgcca ctacaaggga tctgcgtcac atttttcata attcatgttt ttctaataat
     2521 tagaatatta aacaataaca atccattact ggaatcattt ggaatcttta cattatgccg
     2581 tgcacgtctg ctgctacgct ttttgtcatt tgtagcacaa gtaagtgtca gcagtggtgc
     2641 ttcacacttg cccggtaatt aacgacgaaa gaaaagtaag gtggatgaac aatgagtgcg
     2701 tcgttggcga tcctgaccat cggcattgta cctatgcagg aggttttgcc gctcctgacg
     2761 gaatacattg acgaagataa tatttcccat catagcctgc tggggaagtt aagtcgtgaa
     2821 gaagtgatgg cggagtacgc gccagaagca ggcgaagaca ccattctcac attattaaat
     2881 gacaaccagc tggcccatgt ttcgcgtcgt aaagtggagc gagacctgca aggtgtggtt
     2941 gaagtgctcg ataatcaggg ttatgacgtc attttattaa tgagtacagc aaacattagt
     3001 agtatgactg cgcgtaatac gatctttctt gagccgtcgc gaatattgcc tccactggtt
     3061 tcctctattg ttgaagatca tcaggtgggg gttatcgttc cggttgagga gatgctgacc
     3121 gttcaggcgc aaaaatggca aattttgcag aaatcgccgg tattttcatt gggtaacccc
     3181 attcatgatt cagaacaaaa aatcattgat gccgggaaag aattactggc aaaaggggct
     3241 gatgtcatca tgctggattg tttaggattt caccaacgtc atcgcgattt actgcaaaaa
     3301 cagctcgatg ttcctgtctt gctgtctaac gtattgattg cacggctggc tgcggaatta
     3361 ctggtgtaat tttgcgtgac agccagcgcc tctggcccct atagtgaagt agatgttcaa
     3421 ctaccagaca gggccagttt atgcttcaaa gtaatgagta cttttccggc aaagtgaaat
     3481 caatcggctt ttccagcagc agcactggtc gcgccagcgt gggtgttatg gttgaaggcg
     3541 aatacacctt cagcaccgct gagccggaag agatgacggt aatcagtggc gcgctgaatg
     3601 tgttactgcc tgacgcgacc gactggcagg tgtatgaagc cggttcggtg tttaatgttc
     3661 ccggtcacag tgagtttcat ctgcaagttg ccgaacccac ctcttatctg tgccgctatc
     3721 tgtaattcct cgccttcccc ttgaacggga gggcattttt ctgaaatatc ctctctttaa
     3781 cccataataa tatttcct
//
LOCUS       DAZRUF010000303         3796 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZRUF010000303 DAZRUF010000000
VERSION     DAZRUF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.306"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            74..733
                     /locus_tag="ACHXOL_003958"
     CDS             74..733
                     /locus_tag="ACHXOL_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR6810801.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            837..1811
                     /gene="ghrB"
                     /locus_tag="ACHXOL_003959"
     CDS             837..1811
                     /gene="ghrB"
                     /locus_tag="ACHXOL_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR6810802.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1861..2571)
                     /locus_tag="ACHXOL_003960"
     CDS             complement(1861..2571)
                     /locus_tag="ACHXOL_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR6810803.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3005..3295
                     /locus_tag="ACHXOL_003961"
     CDS             3005..3295
                     /locus_tag="ACHXOL_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR6810804.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3576..3788
                     /gene="cspA"
                     /locus_tag="ACHXOL_003962"
     CDS             3576..3788
                     /gene="cspA"
                     /locus_tag="ACHXOL_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR6810805.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
BASE COUNT          997 a          949 c          936 g          914 t
ORIGIN      
        1 ttcatgttca cgcggcataa tctcccgcca cggaacccgt ggcaaagaat aaaaaggtta
       61 ttaaggatta acaatgaaga aacgtgttta tcttattgcc gccgtagtga gtggcgctct
      121 ggcggtatct ggctgcacaa ctaaccctta caccggcgaa cgcgaagcag gcaaatctgc
      181 tatcggcgca ggtctgggct ctctcgtggg cgcgggtatt ggtgcgctct cttcttcgaa
      241 gaaagatcgc ggtaaaggcg cgctgattgg cgcagcagca ggcgcagctc tgggcggcgg
      301 cgtgggttat tacatggatg tgcaggaagc gaagctgcgt gacaaaatgc gcggcactgg
      361 tgttagcgta acccgcagcg gggataacat tatcctcaat atgccgaaca atgtgacctt
      421 cgacagcagc agcgcgaccc tgaaaccggc aggcgctaac accctgaccg gcgtggcaat
      481 ggtgctgaca gagtatccga aaacggcggt taacgtgatt ggttataccg acagcacggg
      541 gggtcacgac ctgaacatgc gtctctccca gcagcgtgcg gattccgttg ccagcgcgtt
      601 gatcacccag ggcgtggacg ccagccgcat ccgtactcag ggccttggcc cggctaaccc
      661 aatcgccagc aacagcaccg cagaaggtaa ggcgcaaaac cgccgtgtag aaattacctt
      721 aagcccgctg taatcccttt catgccaggt gatgcagaaa tcacctggca ttcttcagtt
      781 ttccttcatc atatttcagg ctaaggtgat cgccttatca gtgaatggag agaagcatga
      841 agccgtccgt tatcctctac aaagccttac ctgatgattt actgcaacgc ctgcaagagc
      901 atttcaccgt tcaccaggtg gcaaacctca gcccacaaac cgtcgaacaa aatgcagcaa
      961 tttttgccga agctgaaggt ttactgggtt caaacgagaa tgttgatgcc gcattgctgg
     1021 aaaaaatgcc gaaactgcat gccacatcaa cgatctccgt cggctatgac aattttgatg
     1081 tcgatgcgct taccgcccga aaaattctgc tgatgcacac gccaaccgta ttaacagaaa
     1141 ccgtcgccga tacgctgatg gcgctggtgt tgtctaccgc tcgtcgggtt gtggaggtag
     1201 cagaacgggt aaaagcaggc gaatggaccg cgagcatagg cccggactgg tacggcactg
     1261 acgttcacca taaaacactg ggcattgtcg ggatgggacg gatcggtatg gcgctggcac
     1321 aacgtgcgca ctttggcttc aacatgccca tcctctataa cgcgcgccgc caccataaag
     1381 aagcagaaga acgcttcaac gcccgctact gcgatttgga tacactgtta caagagtcag
     1441 atttcgtttg cctgatcctg ccgttaactg atgagacgca tcatctgttt ggcgcagaac
     1501 aattcgccaa aatgaaatcc tccgccattt tcattaatgc cggacgtggc ccggtggttg
     1561 acgaaaatgc actgatcgca gcattacaga aaggggaaat tcacgccgcc gggcttgatg
     1621 tcttcgaaca agagccactg tccgtagatt cgccgttgct ctcaatggcc aacgtcgtcg
     1681 cagtaccgca tattggatct gccacccatg agacgcgtta tggcatggcc gcctgtgccg
     1741 tggataattt aattgatgcg ttacaaggaa aggttgagaa gaactgtgtg aatcctcacg
     1801 tcgcggacta agccgcgact gcgtgaagta aagcccgata atcgctcggg cttttactct
     1861 ttattgggtt gcagtaactg ctgtagtcca ggcctgatta aacgcctgat gttgtgccgg
     1921 taatggcgca atcagtttgt tatattcact tgcctgctgt gaagtcggga actggatgcc
     1981 atttgcgaca aagctcacct gagtaccttg ctgagcaatg tagtctccaa ccatcaccag
     2041 ttgttgcgtg aaagtttgtg ccgccggaat caacggttgc aatgcatcag ctggcgtcgt
     2101 taccactttg gtgaatgcct gatcaaatac cggtttcagg tcatcgctct gttttagcgc
     2161 agagtgcgca gcatcggcct gcaacttcgc attttgcagt tgttgcgcca gcacacccag
     2221 cgaaccattc atttcacgca gcggaccgct ttgcgtaacg taatcctgcg gtacgcgaat
     2281 tgcgttaacg ctgtctacga ccgggcgcag accagaatcc atcgcctgat tcacctgctg
     2341 agaataacca tacagaatcg cgtaatcaga gacaaaagga ccaaactgtt ttttctgatc
     2401 ggcagtcagg gttggtagac gttcaccgct acgcatcact gtattctgca ggaagtcgat
     2461 aaacgcttta cgctgatcgc cttctttatc aaaacaccca ctcaggctaa ctaccattaa
     2521 taacgccgca agaggcgcaa accagcgaga gcaggacttt cctgtcgcca ttttcttact
     2581 cctttcaccc aaaaaaagcg cacaacgaca cacgcgtgtc tgacgtgaca aggatagtcc
     2641 aggacaggct tgcaggatac cctttctacg taatcttcct ggtaaaatca tggagaaaat
     2701 gaaacaaggt taatttgttg tttcattgtt aaaaaatgat agtgccgttg ccttttttgt
     2761 agccagagat ttacgccttc gcagaaggtt agattttcga aatctttaca aaacaggagt
     2821 ggtaagagat atatcggcat ttatcagatc gttaggtggc tataagtcac gctaaatgac
     2881 aggctgaatc gaatcatagc cagagcatgc cctgacttca ccccgctgtg tctgcttttc
     2941 ccgactattc ttaatgagct tcgatgcaat tcacgatccc gcagtgtgat ttgaggagtt
     3001 ttcaatggaa tataaagatc caatgcatga gctgttgagc agcctggaac agattgtttt
     3061 taaagatgaa acgcagaaaa ttaccctgac gcacagaaca acgtcctgta ccgaaattga
     3121 gcagttacga aaagggacag ggttaaaaat cgatgatttc gcccgggttt tgggcgtatc
     3181 agtcgccatg gtaaaggaat gggaatccag acgcgtgaag ccttcaagtg ccgaactaaa
     3241 attgatgcgt ttgattcaag ccaacccggc attaagtaag cagttgatgg aatagacttt
     3301 tatccacttt attgctgttt acggtcctga tgacaggacc gttttccaac cgattaatca
     3361 taaatatgaa aaataattgt tgcatcaccc gccaatgcgt ggcttaatgc acatcaacgg
     3421 tttgacgtac agaccattaa agcagtgtag taaggcaagt cccttcaaga gttatcgttg
     3481 atacccctcg tagtgcacat tcctttaacg cttcaaaatc tgtaaagcac gccatatcgc
     3541 cgaaaggcac acttaattat taaaggtaat acactatgtc cggtaaaatg actggtatcg
     3601 taaaatggtt caacgctgac aaaggcttcg gcttcatcac tcctgacgat ggctctaaag
     3661 atgtgttcgt acacttctct gctatccaga acgatggtta caaatctctg gacgaaggtc
     3721 agaaagtgtc cttcaccatc gaaagcggcg ctaaaggccc ggcagctggt aacgtaacca
     3781 gcctgtaatc tctgct
//
LOCUS       DAZRUF010000304         3736 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZRUF010000304 DAZRUF010000000
VERSION     DAZRUF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.307"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(10..165)
                     /locus_tag="ACHXOL_003963"
     CDS             complement(10..165)
                     /locus_tag="ACHXOL_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR6810806.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(368..775)
                     /locus_tag="ACHXOL_003964"
     CDS             complement(368..775)
                     /locus_tag="ACHXOL_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR6810807.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            852..1079
                     /locus_tag="ACHXOL_003965"
     CDS             852..1079
                     /locus_tag="ACHXOL_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR6810808.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPWKVIHENQA"
     gene            1063..1484
                     /locus_tag="ACHXOL_003966"
                     /pseudo
     CDS             1063..1484
                     /locus_tag="ACHXOL_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            1562..2361
                     /locus_tag="ACHXOL_003967"
                     /pseudo
     CDS             1562..2361
                     /locus_tag="ACHXOL_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2368..3114
                     /locus_tag="ACHXOL_003968"
     CDS             2368..3114
                     /locus_tag="ACHXOL_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR6810809.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKLGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVERRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            3129..3551
                     /locus_tag="ACHXOL_003969"
     CDS             3129..3551
                     /locus_tag="ACHXOL_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR6810810.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            3552..>3736
                     /locus_tag="ACHXOL_003970"
     CDS             3552..>3736
                     /locus_tag="ACHXOL_003970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810811.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASV"
BASE COUNT          984 a          798 c         1038 g          916 t
ORIGIN      
        1 tcgttcattt tactcacctg aatgtcttcc caaccaacga cgtgcgccag cttcggtttt
       61 aaacgttttg cttttggtat acgtcatggc ggtgaatgtg ccgtcctggt tggggaacac
      121 gccgtacacc agagattcgt tgttgccaag atcgatagta ttcatgttga ccccatttcc
      181 ccttaacgcc ggggtagcgg aactgtttgc tgagaacacc gtgcggtgtc ttgatgaatg
      241 aaatttagaa taacctaagg tgggcggtca agatttttgt gtagaaaaac ctaagttttt
      301 tgatgtaaaa aacacaagtg tttgaaagtt tgtgcttttt attacagggt gtggagaaaa
      361 aaggggatta tttgtttgcg cttcttttgc gagctttgag tagttcttca aaaagtttgt
      421 tgaaattttc aactcgagca cgcatctctg acaacagggc cttttgctct gactcaggca
      481 gtgcgtcgaa cagttgaagt aactcttttt gatcttctgt cagaatggct ggctgattat
      541 ccgggatcgg ttcgcctggt tgtttatctt catccccaaa aagaagccaa gtcggtgagc
      601 actgaagcgc ttggctcagt gcgaataatc ttttccccgc cggctgtgtt tcatctcttt
      661 cccattgaga aattgttacg tgagccactt tgaccagctt acctaatgcg gcctgagaca
      721 gttttaattt tttacgccta tgtaagaggc gagcaccgaa ggtttcgttt ttcatattag
      781 ggaattctaa tttttcttga cttaggtttc tctacaatct agtttcctta ggaaaatcta
      841 aggagctcga tatgttgaaa attgatgcta tagcgttttt tggcagcaaa acaaagcttg
      901 ccaatgccgc aggagttagg ctggcaagca ttgctgcatg gggggaactg gttcctgaag
      961 gtcgcgcgat gcgcctgcaa gaggcatctg gcggggaact tcagtacgac cccaaagttt
     1021 atgacgaata tcgtaaggca aaacgacctt ggaaggtgat tcatgaaaat caggcatgag
     1081 cacatcgaat cagtgctgtt gccctggcag ccgaaaaagg gcaggcgtgg gtcgctaacg
     1141 caattactga agaatatctg cggcaggggg gcggcgaatt gtctctggta ccaggcaagg
     1201 actggaacaa tcagcagaat atctatcacc gttggttgaa aggtgaaacg aaagcgcaaa
     1261 gggaaaaaat tcagaaactg atccctgcgg ttctggcaat tcttccgcgt gagctgcgtc
     1321 accgactctg tatcttcgat accctggaac gccgtgcatt actggcggcg caggaagcgt
     1381 tgagtacggc aattgatgcg catgatgatg ctgtccaggc cgtttaccgg aaagcacatt
     1441 tcagcggtgg tgggtcgccc ggcgattctg tcgtagtgca ttgattgaaa ttaatcgtgc
     1501 cggattgttt tgttcggtat cagttaaatg taacgctgcg agcgttacaa ggtgaaaaca
     1561 aatggcttca aactggataa agctcgaggt tattacgccg gataagccgg aaatattcag
     1621 gcttgctgag attctgaata ttgatccaga tgccgcatta gggaaggtta ttcgcttctg
     1681 ggcatgggcg gatcaacaaa tgatagacgg taatgcagat tgtaacgctc gcggcgttac
     1741 aaaaagtgca atagatcgca tcacttttat gtctggtttt gctgatgcgt taattcaggt
     1801 tggatggctg gtcgaaaatg acggtgggct ttctctacct aactttgaac gtcataacgg
     1861 aaaaagctct aaaaaaaaac gggcggttac aaacgagcga gtaacaaaaa tacgcgaact
     1921 gaaacgaaaa ggtaacgctg ccagcgttac acaaacggat caaaaagcgt taccagagga
     1981 agaggaagag gaagaggaag aggaagagga agatctaaat actgatctcc ccctcgccaa
     2041 aaacgagcgt ctaaaaaatt cgagccggag gctattgagc tgcccgattg gttgccggaa
     2101 acactctggc atgagtgggt ccggttcaga caggcattgc gaaaaccgat tcgaacggag
     2161 cagggcgcta acggggcgat acgggaactg gaaaaattcc gtcagcaggg ttttacacct
     2221 gagcaggtga ttcgacacag catcgccaat gaataccagg gcctgttcgc gccgaaaggt
     2281 gttcggcctg agacgttgct ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc
     2341 ccgccaggct tcagggggta acggaccatg aaaaatattg cgacaggcgg cgttctggaa
     2401 cgcatccgcc gactgacccc gccacatgta accgccccat tcagaacggt agcggagtgg
     2461 cgcgagtggc aacttgctga aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc
     2521 cagttgcggg tggaaaaaat tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc
     2581 tcgtttgcga attaccaggt gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa
     2641 tccatcgccg atgaactgat gaccgggtgt acaaattttg cgttcagcgg aaaacttggt
     2701 accgggaaga atcacttagc ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca
     2761 gtgattgtgg ttaccgtggc tgatgttatg agcgccctgc acgccagcta tgacgacggg
     2821 cagtcaggcg aaaaattttt gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa
     2881 attggcattc agcgcgagac gaaaaacgag caggtggtgc tgcaccagat tgttgagcgc
     2941 cggacagcgt cgatgcgcag cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa
     3001 acattgctcg gcgagcgggt gatggatcgc atggtcatga acggcgggcg ctgggtgaat
     3061 tttaactggg agagctggcg tccgaatgtt agccattcga gggttgttaa gtagtttcag
     3121 gaggatttat ggcgaaacct tttactcccg aacagcggga agaactgaag acgcgaattg
     3181 tggaactcgt gcatcaggac ggtcgggtca cgattcggca gttgtcagat gaaacaggta
     3241 tcagtcgtgc gtctgtcggt cgcttatgca tagaactggt cgcaagtggt gatgtatata
     3301 attctggcta cggcttattc ccgtctgaac aggctcgcaa ggactggcaa agcgcccgca
     3361 aaaaactctc gagagtaaag gtgaggaaac cggttgttgt tgatccggac cttatctggt
     3421 cattacctga cggagaaata cgccgctacg acaggcgcct gaatataatc tgtcgcgagt
     3481 gccggaagag cgaagctatg cagcgtgtac tggctttcta tcagggtaat tttcaggagg
     3541 cgatactgtg aatgaaatta gctatcaggc ttcaattacc gctggcattc gcatcaaagg
     3601 agaggagcat ggaaataaaa ccagaagatg agttaagcaa tatcgtttta tttccggtaa
     3661 aagaggatga ccctcgtaat caggttaatt ttctttatga gccatcggaa agaccatatt
     3721 gccatcacgc ctctgt
//
LOCUS       DAZRUF010000305         3693 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZRUF010000305 DAZRUF010000000
VERSION     DAZRUF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.308"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            78..329
                     /locus_tag="ACHXOL_003971"
     CDS             78..329
                     /locus_tag="ACHXOL_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR6810812.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKVS"
     gene            331..2427
                     /locus_tag="ACHXOL_003972"
     CDS             331..2427
                     /locus_tag="ACHXOL_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR6810813.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRRVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VRQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
     gene            2503..3453
                     /locus_tag="ACHXOL_003973"
     CDS             2503..3453
                     /locus_tag="ACHXOL_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR6810814.1"
                     /translation="MTDIDDVFNTINALINEGDKSQEDIADEAGMSPATLSALRKGEY
                     NGNVEGMHDKLQKWYQVWQKGQNLSGTPGVVKTQTFCDLHELFKTVRSLGIISVVVGV
                     PGVGKTFAAREYCRRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAI
                     RRKLGGGKETSGRVNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADAT
                     RRGTDELARLFSRFAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRV
                     LTHTLNHAWLAAKGAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            3469..>3693
                     /locus_tag="ACHXOL_003974"
     CDS             3469..>3693
                     /locus_tag="ACHXOL_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810815.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT         1016 a          842 c         1032 g          803 t
ORIGIN      
        1 ctttggtatc ttaatcttta tccatacgga tatccgaccg gatatccgcg cgggtaattt
       61 tgtaggttaa aggaaagatg agacaaagag aagatagtga ccgcgattgg ccttcgctga
      121 agattcagct ggcgctcaaa gataacgggt ggtcttcctt ccgggagctt tcactccatg
      181 ctggttatgc tcaggattca ctgaaagcgg tagttcgcac accaaacaaa aaatacgaac
      241 gcgtaatttc tgacgctata ggtgtaccgc cagaaatcat ctggcccagc cggtatcgta
      301 acgatggatt tatcaggaag gtgtcgtgat atgcagtcgt acgttaccgt aaatgacctg
      361 ctcggcgtac caggaatgcc agccaccaca aaaggcatcc gacaggcgtt acagcgtttt
      421 tctcgtgatc ttggtgacgt tagtcgccgt cgggaaggca caaaagccat tgagtaccac
      481 attgattgtc tgcctgaaat aacacgaaag gcactgcgtg aacgctatgt ggaacagctg
      541 gtggctacag agaacaatgt ttctgaagtg aaagctgtta ccagaaaaac acgcaatcct
      601 gacgctgtcc aggcaatcga agcatatcgc ggttctccac aactgatgga agaacgcctg
      661 aatgcgctga ccgaaaacca gcgccgggta tctgaagcaa gagctgcgct ggtggtggaa
      721 gtgctgaagc tggaaagcgc cggtaacctc gggcgactga aagccattaa ctttcttgtt
      781 gaaaaagccc gtaaaggtga gctgccggag cgcctgcaac aggccgcagt taacgccaat
      841 gcaaaacgtg gcgctaatcg tactatcagc cgcgatccac tttaccagtg ggttctgaaa
      901 tacaaccagt cgcagaacgc cgccgaacgg ctgctgttac tggcaccggg aaaacgcgac
      961 gaaataaaac cggaagaaat cagctggctg cctgaatttc tggcgcagta tcggcaggtg
     1021 aacggcagac ccatgtcaga agcctacgag gattttgtcg cagaatggca gcgtcgccat
     1081 gcggacgaac cgtacatgct ggaggtgatg ccttcctatg atgttgtccg gtatgccatg
     1141 aaaaaactgc cggaagtggt gagacaaaaa gggcgcgtta ccggcagtga ataccgtcag
     1201 cttgaaggat tcacccgccg cgactggaca gctatgccgg tgaattatgt ctggattggt
     1261 gacggtcacg gtatgaagct gaaatgtgcg catccgatcc acgggcgtcc attctcaccg
     1321 gaagtcacgt ttgtgatcga tggtggaaca cggtttgttg ttggctggag tcttgacctt
     1381 gccgaaaacg tgttcgccgt tgccggtgcg atacagcacg gcattcgcaa ccacggaaaa
     1441 ccgttcctgt attactcgga taacggttcc ggtgaaacgg ctgacatgct ggacaaagag
     1501 gtcgtgggta ttctgccacg cctggggatt aaacacccta ccggtattgc cggtaatcca
     1561 cagggacgcg gcattattga acgcctgaac cggacactgc cgatgcgtat agccaggagg
     1621 tatcgcacct attttggcaa gggtgctgac cgggaatcgt tgcgtgtact taaccgtgat
     1681 ctgcgttcag cgtttaacgc cctgcaacag gacaagcctc tgaacgaccg ccagaaagcg
     1741 gcaatgcgcg agctgccatc gtgggcggag cttattgagg ctattcgtga aggcgtggaa
     1801 tggtacaaca accgcccgca ttctgagctg ccgatgaaac cgaacggacg gcattacagc
     1861 ccgacagagt tcagaaaaaa acgcctggca gaagaagaca ccgaaattga gtggctgagt
     1921 gatcttgagc tgcgcgacat gttccgccca atggtggaac gaccggtaag acgctgcgaa
     1981 attcagtggc tgaacaatat ttactacgcg ccggagttgc gtgacgaaca cggtcgcaaa
     2041 gtgcttatca gttatgacat tcatgacgcc gagcgaatta cggtacgtcg caaggacggc
     2101 agctttatct gtgaggcgat atggaacggc aataaacgcg cagcctttgc tgtcagtgct
     2161 gaatatcaca aacaacagca gcgtattaaa ggaatgcgca aacgcgctga ggaaaaaatt
     2221 cgtgatgctg aagatgaagg tattcagatt ctggaacaca aacaggctga accctggctg
     2281 agtaatgttt atcgcccggt tggtaatgtg gtggcagttc agcaaccgga atatgaagaa
     2341 gagcatgacg aagaatttga acgcgatttc cggctgggga tgcaaaaact ttttgccatg
     2401 caggaagaag atgatccgct ggcctgaata aaaaaacagt ccgcgggaac ggactgttaa
     2461 ataaaggaaa caaagctgat ttgaaataac cgaggaaata ctatgactga tattgatgat
     2521 gttttcaaca ctattaacgc tttgattaac gagggcgaca agtctcagga agatattgct
     2581 gatgaagccg gaatgtctcc ggcaacactc tcggctctgc gtaaaggaga gtacaacggt
     2641 aatgtcgaag gtatgcatga caaattacag aaatggtatc aggtctggca gaagggacag
     2701 aacttgtcag gtacccctgg tgttgtgaag acccagacat tttgtgattt gcatgagctg
     2761 tttaaaacag tgcgctcact cgggattatc agtgtcgttg ttggcgtacc gggtgtcgga
     2821 aaaacctttg ctgcccggga atattgtcgc aggacagcta atgcctggat ggtgactctt
     2881 gctccggcac attcgagcgt aacggaatgc ctccttgaaa tagcatatgc gctggaaatt
     2941 aataatccgg gaagaaataa ggggaatata acccgcgcca ttcgcagaaa actgggtggt
     3001 ggtaaagaaa ccagtggccg tgttaacccc cttctgatta ttgatgaagc tgatcactta
     3061 agtgtggatg gtctggagca attacgcgcc attcaggatg ccactggcgt ggggatggtt
     3121 ttaattggca acccgaaaca gatggcggat gccacccgtc gtgggactga tgaactggcc
     3181 cgtctgttca gccgttttgc catgacaaaa caactgcgca aagtaaaaaa gaccgatgtg
     3241 atggctgtag ccaaagcatg gggagtaaca caggaagatg agctggacct gctgatgagg
     3301 gtagccgaaa agcccggggg attgcgtgta ttaactcaca cactgaatca tgcgtggctg
     3361 gcggcaaaag gtgcaggcac atccctgaat aaaagtcaca ttaaagcggc ttttaaggag
     3421 gcttacagca accccaggtt actgaactgg tagtaaagga cataaaccat gatgtcacga
     3481 aatataaaaa tggcaacgga tgcgcagaac tggttacagg cgcacgggag tcacgtaaat
     3541 gaatcatatc tcggcgtggc gcgtccggta cttgaaatca cctgcccacc accggagctg
     3601 gtcagaaacg ctgtcaggat tatggagcat aaatcagggg ttgcccgttc agtatggacg
     3661 gcccgcctta atggctgcca gattatctgg cgt
//
LOCUS       DAZRUF010000306         3690 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZRUF010000306 DAZRUF010000000
VERSION     DAZRUF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.309"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            248..1117
                     /locus_tag="ACHXOL_003975"
     CDS             248..1117
                     /locus_tag="ACHXOL_003975"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR6810816.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(1277..1870)
                     /gene="rclC"
                     /locus_tag="ACHXOL_003976"
     CDS             complement(1277..1870)
                     /gene="rclC"
                     /locus_tag="ACHXOL_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR6810817.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(1882..2118)
                     /gene="rclB"
                     /locus_tag="ACHXOL_003977"
     CDS             complement(1882..2118)
                     /gene="rclB"
                     /locus_tag="ACHXOL_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR6810818.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(2227..3552)
                     /gene="rclA"
                     /locus_tag="ACHXOL_003978"
     CDS             complement(2227..3552)
                     /gene="rclA"
                     /locus_tag="ACHXOL_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR6810819.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         1054 a          796 c          778 g         1062 t
ORIGIN      
        1 acattctcca ttaattcttc gtgatatcgc acattttcgt tatgcccagg gccctaagtg
       61 tatcaaaaac agcaaggtcg aaggcaaaag cactggtagc gtctgcttcg ttgtccccga
      121 aaatagagag gtttcagtcc tacattatta atgaattttt tgcataagtg atatcaaaat
      181 ccacatacta atttgaggtt atgttttaac gtagactcat tgttcatgcc taatggaggg
      241 actgacagtg gaattttccg tattaagtaa caatctgaaa atgccgatga tgggatttgg
      301 tgtttttcag gttaccgata aaaacgtgtg caaacagtca gtgttgaacg ctatccgcac
      361 gggttatcga ctcatcgaca ctgcagcggt atacggtaat gaagatgccg tcggtgaagc
      421 cgttcgtgaa gctatatctg aaggtttatg tacccgagat gagttattta taacgtctaa
      481 attgtggggg caggatatgt tgaatcagga tacggcagca gcaggtattg aagcatcatt
      541 aaaaaaatcg ggactagagt acttcgacct ctatttattg caccaggcta tgcgcgatta
      601 tttcagtgtg tggcgtgcac tcgaagatgc ctatgaagag ggcaaattaa aagcaattgg
      661 ggtttccaat ttctatcctc atgttctggc gaacttttgt gaaacggtaa gagttaaacc
      721 gatggtcaac caggtcgagt tgcatcccta ttttgcccaa ccagaggcgc tggcaaccat
      781 gaagtattat aacgtgcagc ctgaagcatg ggctccgtta ggtggtggac gacataaacc
      841 ctttgaaaat aatctgcttc agagtattgc agatgcccat caaaaatcga tttctcaagt
      901 cattctgcgt tggaatattc aacggggagt ggtcgttatt ccgaaatcga cacatcaaca
      961 gcgtatcgaa gaaaattttg ctatctggga tttctcactg acagagaaag aaatggcaca
     1021 aattagttcg cttgatttgg gttatgttgg ggagtcggta aaacatttta atcctgaatt
     1081 tgttcgtggt tgtcttgctg taaaaataca tgattgatat taatcatata ttttacctga
     1141 gacgacaaga atcttttaac aggggagtga tattgatctt cactctgtca tatctccggt
     1201 aatatggcgt caggcttcat catcagaaag ggggcctgac gcctgaaaaa gtgaacaaca
     1261 gacagtgttc ggattatcaa tattcagttt ttaacgttga actggattca ttactgcgtt
     1321 gtttaagaat ttcccgcgcc gaatctgcca ttatcatgac agcacctgcc agcatcagag
     1381 tatctttcaa taccaagcga ccagcaccgg ataaataagg gaaaccatga tgagcgtcac
     1441 ccaatgcggg tacccatgcc tccggggtgg tgattaaaaa tgagagtgtt accagcggcg
     1501 tggtatatgc catcagtcct cccaataaac ctaaccagcg attgacagga ttagccaaaa
     1561 ccagcagagc aataatcacc tccacgacgc caagaccgtt ggaaaaacca taggtattat
     1621 tggccgattg ccatgccctt gcttctggtt tgtattcgcc ttcgtgagtc agatactgtt
     1681 tatagtcttc cgggtgttca taaaagaacg acattagtgg actgtttgcg acgaatggtg
     1741 taatgctgtc tgcctcgtaa gggacaaact ttaataaccc aatccacata aaaacaatcg
     1801 caatactcag acgaatcaat gacaggccaa ttttatctcc ccgacttaac aggtgcaggt
     1861 atttttccat tgtgaacatc cttatttata caatactgct gttgcggttg aattattatc
     1921 aattcgcatc gatgtgactt tccaggatga agcattatat tttctggcta tatcagcaat
     1981 ggtatcctcc agaactgtcg gtgatgctga tactgacgag acgctgatat gtttcagaat
     2041 tattttatca tctgcattgg tggaaaattc cataacgcca gataatagtg ttgcaaataa
     2101 aacagatttt ttaaacatat aaaataccat caatgaagtg attaagaagt tatagagtaa
     2161 cagagaatta ataagattct tttctctgag acgccagaat atttgttctg gcgtctgatt
     2221 ttgagtttat ttgactaatg aaaatagatc attgagtgat tcgctcatcg acggatgagt
     2281 aaatatctga tcgcgtaata tgctataagg cagcccggca tccatcacca ttttcactat
     2341 attgatcatc tcgtgggagt caacacacag cagtgatgcc cctaatatac gttgggtttt
     2401 attatcaaca atcgctttta ataccccacg agtatcattc atcactctgg cacgcggaat
     2461 tgcagctaca ggcaatgtca ccacctgaat atcagcacca ctctctctgg cctgttcttc
     2521 tgtcatacca accctggaca gtggcggtgt catgaatacg gaataaggca catttttccg
     2581 atcatcagta ctacgtctgc cttctcccag caactcatca cgcacaatgc ggtaatcatc
     2641 cagtgaaata taagtaaatt gcagcccgcc ggtaacatct cccatcgccc aaatattgtc
     2701 tgcggtggta tgtaattgct tgtcaacgac aattgccccg cgctcgttta ctgcgatacc
     2761 ggcattttct ggatgtaacg aagcggtagc cggttgacga ccggaagcta ttaacagtgc
     2821 atccaccgcc agttgggcgt gctcgctatg cacttgcact tgattttcat ggtgactgat
     2881 tcgctccaca tgggcattga ggataatatc gacgccctga tcgcgtaaaa tcgtcgcgat
     2941 attatcagca atatcccgat cttcccgagg caaaaacagc gaagctgctt ctaaaatggt
     3001 taccttgctg ccaaaattag cgaacataga ggcgaactca acgccaatat atccgccgcc
     3061 caaaatacct aaatgcccag gcaattcttt tagattaagt aatccggtgc tgtcatatac
     3121 accaggcgtg gtggtaattc caggaattgg cggaaccacg gtttgtgcac cggtattaat
     3181 aaaaattttc tcgccatgaa tttccagatt tccctcaggc cgatgaacac gcaggctatg
     3241 attattgata aactccgcct ggccgtcgat cacgtcgata ttgggcatat ccgcaagatt
     3301 atgaaaattc ttattacgta aaaaattaac cacttcattt ttacgctgta tggcacggac
     3361 aaaatctgtg tgctgctgtg cgtcatgaac caatgttttg gttgggatgc agccgatatt
     3421 aatacaggtc ccgccataca ttgcatttga ttgttcgatg agagccactc gccaacctgc
     3481 ttttgccagc gtgacggcta atgtttttcc agccttgcca aaaccaataa taactgcctg
     3541 atatttattc atgatgattt cctgtcgaaa cacgttcatt tgacatcatg actatagacg
     3601 caaaagagca aataccattt cttcacaacg cttgatattt tgtcttatcg tctggtattt
     3661 tctttttctt aatcagaatc acaaaacaaa
//
LOCUS       DAZRUF010000307         3651 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZRUF010000307 DAZRUF010000000
VERSION     DAZRUF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.310"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            159..1262
                     /gene="proB"
                     /locus_tag="ACHXOL_003979"
     CDS             159..1262
                     /gene="proB"
                     /locus_tag="ACHXOL_003979"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR6810820.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1274..2527
                     /gene="proA"
                     /locus_tag="ACHXOL_003980"
     CDS             1274..2527
                     /gene="proA"
                     /locus_tag="ACHXOL_003980"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR6810821.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2642..2717
                     /locus_tag="ACHXOL_003981"
     tRNA            2642..2717
                     /locus_tag="ACHXOL_003981"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2675..2677,aa:Thr,seq:cgt)
     gene            3323..3481
                     /locus_tag="ACHXOL_003982"
     CDS             3323..3481
                     /locus_tag="ACHXOL_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810822.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT          924 a          873 c          979 g          875 t
ORIGIN      
        1 attaaatctg taataaaagc gtaaacaact gccgctaggc ttgctgatcc cgcgcaacaa
       61 aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta aaacgttcgt
      121 ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc cagacgctgg
      181 tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac cgtgcccata
      241 ttgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg attgttattg
      301 tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa ctgccagcga
      361 ctatcgcctc gaaacaactg ctggcagcgg tagggcagag tcgactgatt caactgtggg
      421 aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc cgtgctgata
      481 tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg ctcgataaca
      541 atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt aaagtcggcg
      601 ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa ctgttgctgc
      661 tgaccgatca aaaaggtttg tataccgctg acccgcgcag caatccgcag gcagaactga
      721 ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac agcgtttcag
      781 gcctcggaac tggcggcatg agtaccaaat tgcaggccgc tgacgtggct tgccgtgcgg
      841 gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat gtgatggaag
      901 gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac cgtaaacgct
      961 ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca actgccgcca
     1021 ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact ggcaatttct
     1081 cgcgtggtga agtcatccgc atttgcaacc tcgaaggccg cgatatcgcc cacggcgtca
     1141 gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa gaaattgatg
     1201 caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg attacccgtt
     1261 aaggagctgg ccgatgctgg aacaaatggg cattgctgcg aagcaagcct cgtataaatt
     1321 agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg atgaactgga
     1381 agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg cgcgagccaa
     1441 tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc tgaaaggcat
     1501 tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg taatcgatgg
     1561 cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg gggttattgg
     1621 cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt gcctgaaaac
     1681 cggtaatgcg gtgatcctgc gcggtggcaa agaaacgtgt cgcactaacg ctgcaacggt
     1741 ggcggtgatt caggacgccc tgaaatcctg cggcttaccg gcgggtgccg tgcaggcgat
     1801 tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat acatcgacat
     1861 gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt cgacaatccc
     1921 ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg tagagatcgc
     1981 tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat gtaatacggt
     2041 tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat taagcaaaca
     2101 aatggcggaa agcggcgtga cattacacgc agatgcagct gcactggcgc agttgcaggc
     2161 aggccctgcg aaggtggttg ctgttaaagc cgaagagtat gacgatgagt ttctgtcatt
     2221 agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata ttcgtgaaca
     2281 cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc agcgttttgt
     2341 taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta ccgacggcgg
     2401 ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg cgcgtggccc
     2461 aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt acaccattcg
     2521 tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc cattgacgca
     2581 tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat gtcctttcag
     2641 ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg taggttcgac
     2701 tcctattatc ggcaccatta agaaaattaa taacttagct taacttctct aagaaattgt
     2761 atcttctgtt atcacttaca aacgcctata cgttgttatg ggctttggta gtgtaacacg
     2821 tatgggcgag tagttcccag gctcaacctc gtaacaagtt cctttgaagc tggaagacgt
     2881 gccaccgtct ctaaaccttc taccaaaact cttagcaaga tgttccacac ctggcgcggt
     2941 gtccaatgat gctacacgtc gtgagacgtt gcagaggata aacaggctaa atgatgggaa
     3001 gagttttaag tgcaatagtg gattaaagcc cacctcttca aaacctaact tgatcgggaa
     3061 agaatcaagc tctagcacgg tttcgtccac cggagtacaa cgggtgcctg ataagagaag
     3121 aactgctaag acttcatagc ttttgtgaaa aagccctttt tagtggatac gcctacggcg
     3181 cttatttttt ggtataacca tcaaaggcgc tttatggctc ctattgtctt gaaaatgtat
     3241 ctttacttgt attgtgtttt atgcgtagtc tccctgtgta gtatgtaact tgttaatttt
     3301 catatgaata cagaggcaca cgatgaaaaa cattgcagca atcaaacgta ataaccgcaa
     3361 gattcacgct cgtaagttcc tgtctacgcc agaaggaaaa gcctggctag agcgtaaaca
     3421 gagagagaac gaagaaagaa aactccttag tgagttgaaa tggcttagag aggatttctg
     3481 aaaatctcca gaacgcaaca ggacgcattc taacgctgtt ttcattactc ggtataggga
     3541 taggtgatgt gtgtttaatg cgtctgtgcg cttgttttgt gccttatttt gcattttcat
     3601 aatgcggaag agaacacgga aaaccttaaa attaattatt ggggtattta a
//
LOCUS       DAZRUF010000308         3632 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZRUF010000308 DAZRUF010000000
VERSION     DAZRUF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.311"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(94..657)
                     /locus_tag="ACHXOL_003983"
     CDS             complement(94..657)
                     /locus_tag="ACHXOL_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR6810823.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(761..979)
                     /locus_tag="ACHXOL_003984"
     CDS             complement(761..979)
                     /locus_tag="ACHXOL_003984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810824.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1138..1524)
                     /locus_tag="ACHXOL_003985"
     CDS             complement(1138..1524)
                     /locus_tag="ACHXOL_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR6810825.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1514..1885)
                     /locus_tag="ACHXOL_003986"
     CDS             complement(1514..1885)
                     /locus_tag="ACHXOL_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR6810826.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1885..2142)
                     /locus_tag="ACHXOL_003987"
     CDS             complement(1885..2142)
                     /locus_tag="ACHXOL_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810827.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2139..3530)
                     /locus_tag="ACHXOL_003988"
     CDS             complement(2139..3530)
                     /locus_tag="ACHXOL_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR6810828.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3527..>3632)
                     /locus_tag="ACHXOL_003989"
     CDS             complement(3527..>3632)
                     /locus_tag="ACHXOL_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR6810829.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          850 a         1016 c          812 g          954 t
ORIGIN      
        1 ccgctcatcg gcgggtttaa gctgtgtggc gtagtaacca ctcttaacag gatattcaac
       61 tttttacgat cgtaaagttt tcggaaaaaa tttttaaaac cgcatcaacc gctccaccag
      121 ttgctcttta cgggcaacga tccacccgtg ttgctccaga taaaatttaa accgttccag
      181 agtacatacc atcgcatcgg cgggtacctt ttccgtgaac tcaacctgac cgtgtttatc
      241 gaagtggctc agtaatgcgc atccatcatt ttcgatagat gtgttttgtg ccgctggtgg
      301 ctgtttctgg ttgaaataac agtcttccag tttttcgaac acttcccacg cctgatcggt
      361 ttcgagcatt ttggcgtgac gggctgcgcc acgttctgtc cagaggatga gggagcgaac
      421 gttacgggca attttcacag aaactcttaa agagttttcg ttcttcaact ctcgtaacgc
      481 atcaccttct atcaggaaaa aatgtttacc ttctataaac cgttctttgt ttcgattgaa
      541 attgtttgtc aaacgctggc gctctgttcc gtacaaatca gccagcagct ctgttgtcat
      601 gacaggaaga tggttatgcg taagtgacgg gagtttttca atagacgtgc gattcataga
      661 catgtccttt cgaatatttt gatttacccc ttttgagagg gtgaccgggc gctcaaaacc
      721 gtcgaaagtc ggcgggcgta tttccattgc tggtattgta ttagccgcac acccggtcat
      781 aaaccaagaa ttctggacac aaaaaaacca catggctatc gggtgtggat accgctttcg
      841 aggtgttttg agcaccgcgg ggaaatgtaa actgcaaacc tgaaactgtc aacaccgtca
      901 ggttccaatc atcgggtggt gagacgcaca gggttggaac taccgggaaa ccgaccggcg
      961 agcttttcag ctcccccatg cgccccacca taattcagat gtgcgcgtgc atacgacaat
     1021 aaaaaacacg ctcgcggcgt gtgtctgtcg cggtctctat ccggggttcc aatcccgacg
     1081 ccagattttg ctggcgcgtg aggaatatag ccccggacaa tgtgtctggt caagcaccta
     1141 cataattcgt tctacgtatc tgtccatctc cagccggata tcaagcatca tcaacatgcc
     1201 atcaataacc ccttccgctt tctgcaggcg cttgccaata caggtatccg aacacccatg
     1261 tttccgtgcc agactcataa aagtcattcc acctacgtaa taatccacca gcaaatcgtg
     1321 caaatcctga tttttcttgt tcaaccgggc catacagcca caaattatca ttgcatcatc
     1381 atcagaacac tgagggcgtg atttcacttt cgacggaatt aaccctttaa aaccagcagc
     1441 gatcgacgcc caggatacat cttcatgagt gtttgcagcc caagcccccc accgttccat
     1501 aacctgctga atatcacgcg ccatcgttat cacctgtgat ttcataaatc ttcacgccca
     1561 gtcgcccacc aggaacgagc tgaccgcgca caatattgat ttcatcaaac tgctcgtcgt
     1621 ctatgagtag tccggcatgc gtcagcgcat ccagtggtgc tttcaggata ttgtccaggt
     1681 cacgacggcg cttatccggt ggctctgcaa taatctttat cgccagcctt ccggacaggt
     1741 ttaatttcag ccgctgctgg cgaacaatta gcgtcacatc acggcgataa cgctcaccaa
     1801 cttttgatac aaaatatgtg ctgccacgac gtcgccagta agtattcacc gtcggcggat
     1861 aaggtaaaat aaactcatgg cgcatcagcg cagcacctcc tgcaccattt tttcaaactt
     1921 tccaactttg gtttccagct ctgccacaca atcaacgagc tcatccaccg ctttttgtgc
     1981 gcgatgtttc gcctgcatca gttccctgag cgctggcacc atatcccgac gaatggcatc
     2041 ttttgttaca cctgtttttt ccagttgttc cgcctgtcgc aacatttcct gcgcgtgttt
     2101 acgcaattgt tcagaggtaa aagtcattgt ctggttgttc aaaagaaacg ctccatctta
     2161 ctgctgtcgg tacgtgtatt gctgtatctg cgcggctggg gctgccgcat tggggtggaa
     2221 agaacctgtg cgctttcctg gtccacaggc agaaaatgtc cgttataaaa acgccggtaa
     2281 atcgtcccca gagaaccgtt acgttgtttc gtgatattaa tttctgctat gcctgtagcc
     2341 tgtgtatccg ggttgtacac ttcatccctg taaagcatca gaatgatgtc tgcatccgcc
     2401 tctatttctc cggaattttt caggtctgag ttcatgggac gtttattggg tctggactcc
     2461 acaccgcggg agagctggct cagcgcaatc aacggaaaac caccggattt tgccaggcct
     2521 ttaagcccct ttgagatttc acccacggca aggtcatgac gccccgtggt tcgggttttt
     2581 atcagcccga gataatcaac caccaccagc gccgtttccg gatgtttaat cagatggtgt
     2641 ttcgttgttg cgcatatctc atcaatggtc aggttcgcct ggtccaccat ccagatattg
     2701 cgcccggtca tccgccccac cccttgtgag aaacgcgccc agtcttcatc ttcaaagtga
     2761 gccacagatt tcaggcgtga tactggcatc cctccagccg cagacaccat acgttcacca
     2821 atctggatgt tcgccatctc catggtgaac agaagcacac catgcccctg ctcagtcacc
     2881 ttgtcgatga tgtccagcgc aagttcggtt ttccccatcg aaggacgggc ggcaatgaat
     2941 accaggtctc cgggctccat accgcctgtt tttgcgtcca gttcatcaat accggtcatc
     3001 aacgtcctgg atttctccag cccctgattc cggcattcaa cacgctcaac cacttccgga
     3061 agcacatcat caatatgtac cggctgaatg acgccctttc ctgtcgacag tgtgaccatc
     3121 atgttctgcg catccttcag ggcatcttca gctgcttcac aggtatgcgc atcacgtaat
     3181 ttctgcagcg cctcattcag tgttttttct gcatcgcgca gtgcggcatt gcgccgcaac
     3241 gctgcaacat agtgctccag tgaagacttc acccaggttt tacgcccggt atcagtaatc
     3301 accggggcaa gttccggcat ctcattacac aacagcacgg ggtcaatcac tcctgaaaca
     3361 cgggcctgtc tgcagatgcc tgtgtagata tcccgatacg ctcgtacaga aaaaatgtcc
     3421 gccggtagtg tgatcagaat atccatcact tcatgatctg ccccacgcag aaagaatgcg
     3481 ccaatgacag cgccttccag gtcatcatta cgccagactg gtgttgtcat gcagccacac
     3541 ctctgataca agaacggtag ctgggccagt tgaacgacaa ccagttgcgc cccccgtctg
     3601 tgatcctgtc ggcaatgcgg gggctgatga ac
//
LOCUS       DAZRUF010000309         3595 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZRUF010000309 DAZRUF010000000
VERSION     DAZRUF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.312"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(86..1483)
                     /gene="ccp"
                     /locus_tag="ACHXOL_003990"
     CDS             complement(86..1483)
                     /gene="ccp"
                     /locus_tag="ACHXOL_003990"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR6810830.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1888..3537
                     /locus_tag="ACHXOL_003991"
     CDS             1888..3537
                     /locus_tag="ACHXOL_003991"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR6810831.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          916 a          929 c          853 g          897 t
ORIGIN      
        1 aacatttaac atgataatat taaaaacaga ctttattatc gtaataaaaa tcgcccaaac
       61 attcctgttc gggcgatcaa attaattatt gtttatcctg catatacggc gtgtacaccc
      121 cgttcagact gtgcaggaaa gctacgatat catccacatc ctcctgcggc agctctttgc
      181 ctacctgata gcgcagcatc agtttcaccg ccccgtccag cgtcggcacg tcaccgcgat
      241 ggaagtacgg tgccgttaaa gcaacgttac gtaaaccggg tactttctga cgcaatttat
      301 cacgctcttc tttagtaaca ttcatacgac caatatccgc cgccgtaatt tccccaaagt
      361 taaagtcttt tttcagcccc aacggttcaa aggaacgtcc accgagaata ataccaccat
      421 gacaagttgc acatttatta tctttaaata attgatagcc ttttttctgt tgcgccgtca
      481 gcgcattttc atctccacgc aaccatttat caaatgggga atccggcgta attaatgttt
      541 tctcaaattc agcaatggca tcagtaatat tttcgccact gaaaccttgc ggatagacgc
      601 cgaggaactg cgctttaagc tgcggatctt tttccagctt agcaataatt tcgtcccaag
      661 atttcgacgc catttcaatc gggttcaacg gcggtccacc agcctgatcc tgcaatgttg
      721 ccgcacgacc atcccagaac tgctcaacgt taaatactga gttaaatacc gtcggcgcgt
      781 taatcggccc aactgcgcca ccaacaccaa tcgatgtttt tctgccatcg acgccccccg
      841 cattcaacgc atggcaatga gcgcatgaaa tggtgctatc agccgataaa cggggatcgt
      901 gatacagcgc aaagcccaac gccacttttt gcgcatcggt aggcagtttt tgcgggatgg
      961 gctgcaccgg ttcattgcga tgctccggag cagtatcatt gctggcgtaa tattccgcgc
     1021 gctgttttgc aatccaggcc agtatttccg cccgctcttc atcactcacc ttacccgccc
     1081 agtgaagcgc ggtataacgc gttggcggca tagtttcata ctgcatcacc cattcaatct
     1141 tattcagatc gctttgcgaa acgggtttat cagccagcag tgccgcacgc acggcctcga
     1201 ggttaaaaga tttatatcca agcttaatgt cgtaatccat caactgtttc gcgccaggaa
     1261 tataataata ggcgggtaat tctgccgaag gcgtgtggca atagtcgcat cctttttcgc
     1321 ggagaaaacc taaaacctta ttattttcac tgacagcgga tgcctgaaca tcggcctgtt
     1381 tactgcgttt attatcgtgg taccacacat aaccagataa ccctaaatag caaatcgcga
     1441 cgccagccag gccgatcgcg gtaatacgtg agaccatttt cattattctt ccctcacggt
     1501 ggtgagttat tgtgacccag aaaattttcc agaatgctgc aaacgtctct gcgagacaaa
     1561 gccatcataa aaaacagtga gatacgcttt ttgataggaa tcaatcaatt acatgaatat
     1621 aaactatcaa taagatagcc tgtgcctatt acccagatca ggaatggcat atcatgattg
     1681 cggcgagagc aggattgaat gttctgaaaa tgaaaaacaa cacgaaatcc gtatttttat
     1741 aaattccatg gatattagtg cgttatattt tttgacaggt taataaacgt gatccaccgc
     1801 acgctttgtc gcccaccagg cggagcgaat gactaccctt aaagaaaagc ccgataatta
     1861 gcgacgaatt tcggaggttg gatccttatg ctcaatcaga aaattcaaaa ccctaatcca
     1921 gacgaactga tgatcgaagt cgatctctgc tatgagctgg acccgtatga attaaaactg
     1981 gatgagatga tcgaggcaga accggaaccc gagatgattg aagggctgcc cgcctctgat
     2041 gcgctgacgc ctgccgatcg ctatctcgaa ctgttcgagc atgttcagtc ggcgaaaatt
     2101 ttcccggaca gtaaaacctt tcccgactgc gcacctaaaa tggacccgct ggatatttta
     2161 atccgctacc gtaaagtgcg ccgtcatcgt gattttgact tgcgcaagtt tgttgaaaat
     2221 cacttctggc tgccggaggt ctactccagc gagtatgtat cggacccgca aaattccctg
     2281 aaagagcata tcgaccagct gtggccggtg ctaacccgcg aaccacagga tcacattccg
     2341 tggtcttctc tactggcgct gccgcagtca tatattgtcc caggcggccg ttttagcgaa
     2401 acctactatt gggactccta tttcaccatg ctggggctgg cggaaagtgg tcgggaagat
     2461 ttgctgaaat gcatggccga taacttcgcc tggatgatcg aaaactatgg tcacatcccc
     2521 aacggcaacc gcacctatta tttgagccga tcgcaaccac cggtttttgc gctgatggtg
     2581 gagttgtttg aagaagatgg tgtacgcggt gcgcgccgct atctcgacca ccttaaaatg
     2641 gaatatgcct tctggatgga cggtgcagaa tcgttgatcc ctaatcaggc ctatcgccat
     2701 gttgtgcgga tgccggacgg atcgctgctc aaccgttact gggacgatcg cgacacgccg
     2761 cgtgacgaat cctggcttga ggacgttgaa accgcgaaac attctggtcg cccgcccaac
     2821 gaggtgtacc gcgatttacg cgcgggggcg gcctccggtt gggattactc ttcccgttgg
     2881 ctgcgtgata ctggtcgtct ggcgagcatt cgtaccaccc agttcatccc catcgatctg
     2941 aatgccttcc tgtttaaact ggagagcgcc atcgccaaca tctcggcgct gaaaggcgag
     3001 aaagagacag aagcgctgtt ccgccagaag gccagtgccc gtcgcgatgc ggtaaaccgt
     3061 tacctctggg atgatgaaaa cggcatctac cgcgattacg actggcgacg cgaacaactg
     3121 gcgctgtttt ccgctgccgc cattgtgccg ctctatgtcg gcatggcgaa ccatgaacag
     3181 gccgatcgtc tggcaaacgc cgtacgcagc cggttactga cacctggcgg gattctggca
     3241 agcgagtacg aaaccggtga acagtgggat aaacccaacg gctgggcacc gttacaatgg
     3301 atggcaattc agggatttaa aatgtatggc gatgaccttc tgggtgatga aatcgcgcgc
     3361 agctggctga aaacggtgaa tcagttctat ctggaacagc acaaaatgat cgagaaatac
     3421 catattgccg atggtgttcc ccgcgaaggc ggcggtggcg agtatccgtt gcaggatggg
     3481 tttggctgga ctaacggtgt ggtacgccgt ttaattggtt tgtacggcga accataatat
     3541 ttttacagcc agccgctaac ttcctgctgg ctgtaaaatt atcctcttca ggagg
//
LOCUS       DAZRUF010000310         3557 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZRUF010000310 DAZRUF010000000
VERSION     DAZRUF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.313"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..2906
                     /gene="sigA"
                     /locus_tag="ACHXOL_003992"
     CDS             <1..2906
                     /gene="sigA"
                     /locus_tag="ACHXOL_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR6810832.1"
                     /translation="KQELSSIIKDKDLSVSGGGELTLKQDTDLGIGGLIFDKNQTYKV
                     YGKDKSYKGAGIDIDNNTTVEWNVKGVAGDNLHKIGSGTLDVKIAQGNNLKIGNGTVI
                     LSAEKAFNKIYMAGGKGTVKINAKDALSESGNGEIYFTRNGGTLDLNGYDQSFQKIAA
                     TDAGTTVTNSNVKQSTLSLTNTDAYMYHGNVSGNISINHIINTTQQHNNNANLIFDGS
                     VDIKNDISVRNAQLTLQGHATEHAIFKEGNNNCPIPFLCQKDYSAAIKDQESTVNKRY
                     NTEYKSNNQIASFSQPDWESRKFNFRKLNLENATLSIGRDANVKGHIEAKNSQIVLGN
                     KTAYIDMFSGRNITGEGFGFRQQLRSGDSAGESSFNGSLSAQNSKITVGDKSTVTMTG
                     ALSLINTDLIINKGATVTAQGKMYVDKAIELAGTLTLTGTPTENNKYSPAIYMSDGYN
                     MTEDGATLKAQNYAWVNGNIKSDKKASILFGVDQYKEDNLDKTTHTPLATGLLGGFDT
                     SYTGGIDAPAASASMYNTLWRVNGQSALQSLKTRDSLLLFSNIENSGFHTVTVNTLDA
                     TNTAVIMRADLSQSVNQSDKLIVKNQLTGSNNSLSVDIQKVGNNNSGLNVDLITAPKG
                     SNKEIFKASTQAIGFSNISPVISTKEDQEHTTWTLTGYKVAENTASSGAAKSYMSGNY
                     KAFLTEVNNLNKRMGDLRDTNGEAGAWARIMSGAGSASSGYSDNYTHVQIGVDKKHEL
                     DGLDLFTGLTMTYTDSHASSNAFSGKTKSVGAGLYASAIFDSGAYIDLISKYVHHDNE
                     YSATFAGLGTKDYSSHSLYVGAEAGYRYHVTEDSWIEPQAELVYGAVSGKRFDWQDRG
                     MSVTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGE
                     KRIKGEKDGRILMNVGLNAEIRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
     gene            2965..3534
                     /locus_tag="ACHXOL_003993"
     CDS             2965..3534
                     /locus_tag="ACHXOL_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810833.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVSHMLVTDTITE
                     ATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLFQGKM
                     STDGNNYYIIKGKVSSVLLLLKMLQINHA"
BASE COUNT         1143 a          742 c          788 g          884 t
ORIGIN      
        1 aaaaacaaga gttatcatcg attataaaag ataaggacct gtctgtctca ggaggagggg
       61 aattaacgct caagcaggat accgatcttg gcattggcgg gcttatattc gataagaacc
      121 agacatataa agtgtacgga aaagataagt cttataaagg tgccgggata gatattgata
      181 ataataccac cgttgaatgg aatgttaagg gcgttgccgg agataatctg cataaaatag
      241 gtagtggtac tctggatgta aaaatagcac agggaaataa ccttaaaata ggtaatggga
      301 ctgtcatcct tagtgctgaa aaagccttca ataaaattta catggccgga ggtaaaggta
      361 cggtaaaaat aaatgccaaa gacgctttaa gcgaaagcgg taatggcgaa atctatttta
      421 ccagaaatgg cggaacactg gatctaaacg gctatgacca gtcatttcag aaaatcgcag
      481 caacagatgc gggaacaacc gtaacgaact caaacgtgaa gcaatcaaca ttatcactta
      541 ctaatactga tgcatatatg taccatggga atgtatcagg taatataagc ataaatcata
      601 ttatcaatac tacccagcaa cataacaata atgccaatct gatctttgat ggctcagtcg
      661 atatcaaaaa cgatatctct gtccggaatg cacagttaac attacaagga catgcgacag
      721 aacatgccat atttaaagaa ggcaataaca actgtccaat tcctttttta tgtcaaaaag
      781 actattctgc tgccataaag gaccaggaaa gcactgtaaa taaacgttac aatacggaat
      841 ataagtccaa caatcagata gcctcttttt cccagcccga ctgggaaagt cgtaaattta
      901 atttccggaa attaaattta gaaaacgcaa ccctgagtat aggccgggat gctaatgtaa
      961 aaggacacat agaggctaaa aactctcaaa ttgttctggg aaataaaact gcatacattg
     1021 acatgttctc aggaagaaac attactggcg aaggttttgg attcagacaa cagcttcgct
     1081 ccggggattc agcaggcgaa agtagtttca acggcagtct gagtgctcaa aacagcaaaa
     1141 taactgttgg tgataaatca actgttacta tgactggtgc attatcctta attaatacag
     1201 acctgattat caacaaagga gctactgtta ccgcccaggg aaaaatgtat gtagataaag
     1261 ctattgaact ggccggaacc ctgacattaa caggcacccc tacagaaaat aataaataca
     1321 gcccggcaat ctatatgtca gatggatata atatgacaga agatggtgcc acgttaaagg
     1381 ctcaaaatta tgcctgggtc aatggtaata taaaatcaga caaaaaagca tctattctgt
     1441 ttggtgttga ccagtataaa gaagataacc tggacaaaac cacacacaca ccgctggcta
     1501 caggtttgct gggtggcttt gatacttctt ataccggagg tattgatgct cctgctgcct
     1561 cagccagcat gtataacacc ttatggagag taaacggaca gtcagccctg caatcattaa
     1621 aaacccgcga cagtcttttg ttgtttagta acatagagaa ttcgggtttc catactgtga
     1681 cagtaaacac actggatgcc actaatactg ctgtgattat gcgggctgat ctgagccagt
     1741 ctgtaaatca atcggataaa ctcattgtta aaaatcagtt aaccggaagc aataacagtc
     1801 tgtcggtcga tatacagaaa gtgggaaata ataactcagg attaaacgtt gacctgataa
     1861 cagccccaaa aggaagcaat aaagagatat ttaaagccag tactcaggcc ataggtttca
     1921 gcaacatatc tcctgtgatc agcacgaaag aggatcagga acataccacg tggaccctga
     1981 ccggatataa ggtggctgaa aatacagcat cttccggtgc agcaaaatcg tatatgtccg
     2041 gtaattacaa agccttcctg acagaagtca acaacctgaa taaacgaatg ggggatctgc
     2101 gtgacaccaa tggcgaggcc ggtgcatggg cccgcatcat gagcggagca ggttcagctt
     2161 ctagtggata cagtgacaac tacacccatg tgcagattgg tgtggataaa aaacatgagc
     2221 tggatggact tgaccttttc actggtctga ctatgacgta taccgacagt catgccagca
     2281 gtaatgcatt cagtggcaag acgaagtccg tcggggcagg tctgtatgct tccgctatat
     2341 ttgactctgg tgcctatatc gacctgatta gtaagtatgt tcaccatgat aatgagtact
     2401 cggcgacctt tgctggactc ggaacaaaag actacagttc tcattccttg tatgtgggtg
     2461 ctgaagcagg ctaccgctat catgtaacag aagactcctg gattgagccg caggcagaac
     2521 tggtttatgg ggccgtatca ggtaaacggt tcgactggca ggatcgcgga atgagcgtga
     2581 ccatgaagga taaggacttt aatccgctga ttgggcgtac cggtgttgat gtgggtaaat
     2641 ccttctccgg taaggactgg aaagtcacag cccgcgccgg ccttggctac cagtttgacc
     2701 tgtttgccaa cggtgaaacc gtactgcgtg atgcgtccgg tgagaaacgt atcaaaggtg
     2761 aaaaagacgg tcgtattctc atgaatgttg gtctcaacgc cgaaattcgc gataatcttc
     2821 gcttcggtct tgagtttgag aaatcggcat ttggtaaata caacgtggat aacgcgatca
     2881 acgccaactt ccgttactct ttctgataac agcccgggcc gcgtttgcgg cccttcttct
     2941 accgtagaga atatgtatta ccctgtgaca gactatatcg ctcttgctct cattattagc
     3001 tttctttttc tgacattatt tatctgcctg ttatgcctta aacatgagcg aataaaaaaa
     3061 gaaactatca ggcaaaaaaa tgcacatatt ctggagcatg gctggaatgc aactgagttc
     3121 tcatggttcc gatacggaca gtataacgaa acgggtattt acatctcgat cgagaagacc
     3181 attatcatta caatcaccgt ttctggtgag tgctttaaaa aagaatacag cattgtctcc
     3241 cacatgctgg tcactgacac cataacagag gccaccctgt acgaaaacgg gctgtatacg
     3301 cgccacatca gactcagtcg cccagtatct gacagcaaat accccttgcc acccggatct
     3361 cagctcatta aaaatatgac cttacgactg cgcctgcagg atcagcagga agaaacatcc
     3421 gtgacattat ttcagggaaa aatgagtacc gacggaaata actattacat catcaaaggt
     3481 aaagtaagct cagtacttct gttattaaaa atgctgcaga ttaatcacgc ctgatctctg
     3541 tcccggtatt ttgaggt
//
LOCUS       DAZRUF010000311         3508 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZRUF010000311 DAZRUF010000000
VERSION     DAZRUF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.314"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..95
                     /locus_tag="ACHXOL_003994"
     CDS             <1..95
                     /locus_tag="ACHXOL_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR6810834.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            244..1491
                     /locus_tag="ACHXOL_003995"
     CDS             244..1491
                     /locus_tag="ACHXOL_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR6810835.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2020..2220)
                     /gene="torI"
                     /locus_tag="ACHXOL_003996"
     CDS             complement(2020..2220)
                     /gene="torI"
                     /locus_tag="ACHXOL_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR6810836.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2343..2687)
                     /locus_tag="ACHXOL_003997"
     CDS             complement(2343..2687)
                     /locus_tag="ACHXOL_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810837.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2929..>3508
                     /locus_tag="ACHXOL_003998"
     CDS             2929..>3508
                     /locus_tag="ACHXOL_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810838.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQ"
BASE COUNT          876 a          715 c          812 g         1105 t
ORIGIN      
        1 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
       61 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc cgcttgcagt
      121 aacggcgaaa ggggcaatga cagcgctgcc atgtcgacaa gaactggaat agtgagaagt
      181 aaacggcgaa gtcgctctta tctctaaata ggacgtgaat tttttaacga caggtaggta
      241 attatggcac tgaatattcc attcagaaat gcgtactatc gttttgcatc cagttactca
      301 tttctctttt ttatttcctg gtcgctgtgg tggtcgttat acgctatttg gctgaaagga
      361 catctaggat tgacagggac ggaattaggt acactttatt cggtcaacca gtttaccagc
      421 attctattta tgatgttcta cggcatcgtt caggataaac tcggtctgaa gaaaccgctc
      481 atctggtgta tgagtttcat cctggtcttg accggaccgt ttatgattta cgtttatgaa
      541 ccgttactgc aaagcaattt ttctgtaggt ctaattctgg gagcgctctt ttttggcctg
      601 gggtatctgg cgggatgcgg tttgcttgac agcttcaccg aaaaaatggc gcgaaatttt
      661 catttcgaat atggaacagc gcgcgcctgg ggatcttttg gctatgctat tggcgcgttc
      721 tttgccggca tattttttag tatcagtccc catatcaact tctggttggt ctcgctattt
      781 ggcgctgtat ttatgatgat caacatgtgt tttaaagata aggatcacca gtgcgtagcg
      841 gcggatgcgg gaggggtaaa aaaagaggat tttatcgcag ttttcaagga tcgaaacttc
      901 tgggttttcg tcatatttat tgtggggacg tggtctttct ataacatttt tgatcaacaa
      961 ctttttcctg tcttttatgc aggtttattc gaatcacacg atgtaggaac gcgcctgtat
     1021 ggttatctca actcattcca ggtggtactc gaagcgctat gcatggcgat tattcctttc
     1081 tttgtgaatc gggtagggcc aaaaaatgca ttacttatcg gtgttgtgat tatggcgttg
     1141 cgtatccttt cctgcgcgct gttcgttaac ccctggatta tttcattagt gaagctgtta
     1201 catgccattg aggttccact ttgtgtcata tccgtcttca aatacagcgt ggcaaatttt
     1261 gataagcgcc tgtcgtcgac gatctttctg attggttttc aaattgccag ttcgcttggg
     1321 attgtgctgc tttcaacgcc gactgggata ctctttgacc acgcaggcta ccagacagtt
     1381 ttcttcgcaa tttcgggtat tgtctgccta atgttgctat ttggcatttt cttcctgagt
     1441 aaaaaacgcg agcaaatagt tatggaaacg cctgtacctt cagcaatata gacgtaaact
     1501 tttccggttg ttgtcgatat ctccctatcc ctcaaccgga aaataataat actaaagtgc
     1561 ttagcccggc taataatcac ctaatccaaa cgcctcattc atgttctggt acagtcgctc
     1621 aaatgtactt cagatgcgcg gttcgctgat ttccaggaca ttgtcgtcat tcagcgacct
     1681 gtcccgtgta tcacggtcct gcgaattcat caaggaatgc attgcggagt gaagtatcga
     1741 gtcacgccat atttcgctat caggattctg tgtgatggtt acatcgcccg gcccagggct
     1801 gtttagtcat cagcgctttc tgacagtgct gagatttcaa cctgttgcag taaaaatgag
     1861 tagatataag gcaagtgtgc tgccaaaccc atcttttacg gggtgaaggt agatttcgtt
     1921 tgaagggtat ctggtgtccc ctgcagacat ctacttgagg cggcagggga ttgattggaa
     1981 tgctgttttt tagatttgaa aaatatttta cccgctattt tacccattgg cgcggcttaa
     2041 gagcttattt ttgaattcac aatggtcacg atataaccat cttgctcgtc cgtggataac
     2101 tttggcttta ggcaggtcgc cggacttaat ccggtcatag atgaaggtct taccgaagcc
     2161 agtatcagcc atgatgaatt tcaaatcaac cagtgaatca ggttgtagtt cgtgttgcat
     2221 gagtgctatc tccgaatatg gaatcgaacc tgcaaatcag gcaataaaaa gccgcattga
     2281 tgcagcaatg gtaggtctgg atatcttgag aaatgaacag gcctcatcga gtgtgaggcg
     2341 ggttagtcct tgcgtagctc gctgattctt ctgtaagtct ctggtgcttt gttcccgtac
     2401 gtcttcattt cagacttcaa cagagcaacg agtgaatccc attcgttgag gattcctttg
     2461 aatgccggaa cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg aatctgctca
     2521 acaagtgcaa cgcatcgccg aaagtctgct gcgtcatggg gagcgccgaa gtgatgacca
     2581 tagatattct ttttcaggcc acatgcgatt gaggcaagag ttgcgctact gatgccgaca
     2641 tcgccagttg attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccat
     2701 aaaacaaaac tcgccgtagc gagctcagat aaaagaaatc cccgtcagtg cgaggatgct
     2761 gttcattgct gctatacact tttttgctct caacgtaagc ggtagcccat tctgttgggt
     2821 tggtcagttg cttttaggaa atgctcttta ccccttaaac gtcggctgaa agagctaaaa
     2881 tccatgcaaa aaatttacgc aattttgtgt attattgtgc agtaagtaat gagctatttt
     2941 ctgtgcaaaa aatggatgat aaatttgtcc ggggcaggaa aaattttatg ggcgctaaac
     3001 atgaaaaaag attcgtatcc ttatttgatt tgcatgacag tttcagggct gatctttatt
     3061 ttccttttct tctggtggcg ggcagatatc tacagggtca cgtttcttaa tcagagtata
     3121 tcccactatt acattctgtt tagcatggga atagcttttc tgttatctct gttttgggtt
     3181 aagaagggga tagtaaaaca aagcggctgg aagagtctgt cagcatacct taaggtttat
     3241 gcagggatat gcatattttc tggatttttt ctgattatac cccttacaac actaacttat
     3301 tttttgcctg gagagacatc gtcttatgtt gcaccgtatc ggtatacttc cggtagttca
     3361 aaaagttgtt ctggagctga ggtggatgac cccgatctac atgagaatat tcgcatttgc
     3421 tatccgtatg gcaattatga gtacgataat attatctatg ttgaaaagaa aattaataca
     3481 ttaggtgcgg tagtgacata tgcacaga
//
LOCUS       DAZRUF010000312         3465 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZRUF010000312 DAZRUF010000000
VERSION     DAZRUF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.315"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(176..895)
                     /gene="dcuR"
                     /locus_tag="ACHXOL_003999"
     CDS             complement(176..895)
                     /gene="dcuR"
                     /locus_tag="ACHXOL_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR6810839.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(892..2523)
                     /locus_tag="ACHXOL_004000"
     CDS             complement(892..2523)
                     /locus_tag="ACHXOL_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR6810840.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2704..2934
                     /gene="yjdI"
                     /locus_tag="ACHXOL_004001"
     CDS             2704..2934
                     /gene="yjdI"
                     /locus_tag="ACHXOL_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR6810841.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2946..3218
                     /locus_tag="ACHXOL_004002"
     CDS             2946..3218
                     /locus_tag="ACHXOL_004002"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR6810842.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KWQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT          893 a          783 c          865 g          924 t
ORIGIN      
        1 gttagcgttt tgaaattaaa aacaccgttc acctgaagag atattaattt ttagcgatga
       61 tgcagggata attatatttg atctggcaca agttttactg atgaaggatg taacttgtgc
      121 caggggtatt ttgcattacg gtaataatta ttacttacag ataaccagcg atccgttatt
      181 ggcaatattg tttcagtagt gagtagtgtt ctgcctgaat acggtaacgg taaactggac
      241 gccccgtgac gccataatgg atactggtga acaagatgtg gcagttgacc agccagatga
      301 ggtatttacg gcaggaaaca cgcgaaatgt taacctcgtt ggctagctcg tcggttgaaa
      361 attcatagtc ctgatgcgcg tcaatccact ggcacagtgt gcgtaacgtc tgcggcgtta
      421 agccttttgg caagcgacga ggatcctgtt cgttggagct gctgccgtgg attagctgat
      481 caagctcggc ctggtcataa tactgatgtt tttccagcgc cattttcttt tgccgccagt
      541 cggtgagcgc ctcttcaaag cgggaagcct ggaagggttt gatcaggtaa tccacgacac
      601 cgtaatgcag cgaatcttta atggttgccg catcggctgc ggaggagatg acaatcacat
      661 cacttttgca acgcgcgtta tgcaggacag gcagtaaatc gagcccgttc tctttttgca
      721 tatagatatc gagcaatatc aggtcgatag gcgtatcgct attgaagata atctctttgg
      781 ctttctccag cgtcgaggct gttccacagc attgaaagcc tgggatttgt gctacgtatc
      841 ggcgattcag ctccgcgacc attgcgtcgt catcgataat taatacattg atcatctgtt
      901 cgacctctcc ccgtcccagg gtatctggac aaaaaattgt gtgaaaatcc cgggttccga
      961 ttccacggcg atgctgccgc cgagattttc tacctgttgt ttgacaagtg ctaaaccgac
     1021 gcctcgctcg cttccttttg tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg
     1081 tgcgatcccc ggtccatcat cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa
     1141 tgttacgcta atttcgccac cgggttccgg ccctaatgct tccagcgcgt tttctatcag
     1201 atttcccaat gtggtaatca gcgtcgcgac ctggtcctca ctgccgctgt ctggcagctg
     1261 gctttcactg tttaaaatca gcgtatggcc taaatcggtc gcgcgattaa tcttgctgat
     1321 taaaaaacca gcgataaccg gagatttgat cttacccagc agagagccaa tctcttcctg
     1381 atagttattg gctgttttta gaatgtaatc ttccaactgc ttataactct tcagatgcaa
     1441 taatccgaga atcacgtgca atttattcat aaattcgtgg gatcgttcac gaagtgcgtc
     1501 ggcatagttg accagaccgt cgagtcgctg catcagttta cgtacttcgg ttttgtccct
     1561 gaaggttgaa atggcaccga tgataacgcc attactgcgc accggaacgg tgttgatcag
     1621 taatagccgg tctttaatcg taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac
     1681 ttccgagaca tctaccacct gtgaccatga gtggctgagc gtcgacagtt tctcatcgtc
     1741 ctgcgactta cggtaattca gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc
     1801 gcgatcgtcc acggcaacga cgccttcttt gatagactgc aacatggcct ggcgttgctc
     1861 aaacagcgtg gagatttcgt agggttccag gccgaaaagg atttttttca gtaccttaac
     1921 cagaatgcag gtgccaatca gtccgaccag catgccaaat aataccgacc agataatgct
     1981 ccagcgactg tcattgattt gttgggtcac gcggcttaac tcaaggccga tcgccaccac
     2041 gccaatttgt ttatgatttt catcgtagat gggggtaaat acgcgtaaag cctgcgccag
     2101 aaaaccgcga ttgatagcga cattttcttt gccattcagc gctttaagga tgtcatcacc
     2161 tttaaatggc tgaccaatac gctgggcttc aggatgcgag tagcgaagac tttgcatatc
     2221 ggtaacgaca ataaacagca gatcgttgcg tttgcgcacg gcttccgcga tggcctggat
     2281 gccgctctcc tgcggttttt tctgcaagcc ctgacggatt tccggcgagt tggcgagggt
     2341 acgcgccact gccagtgcct tgttggctag cccgtctcgc gtcatatcac tgatttgcga
     2401 gaagtaaatc agatgcacca ccaatagcac cgagaacagt accgcactga ccattaagat
     2461 cactgtggta ctcaatttca tcggacgttt gcgtaacatg cggtagggca atgaatgtct
     2521 catcagcttc cttgtgtgac aaatttctta aacattatct ctgatgaggc gggtaattca
     2581 aagggagtaa gaatgattgg ctatataggg gaagagattc tggcaacgga aactgccagt
     2641 gctgtatgaa gattccaggg ctatgcttat agcgataatc atactgatga gagagggaaa
     2701 gtcatggatc aggcgctact ggacgagggt tatcgctgtt ataccggcga aaagatcgat
     2761 gtctatttca acactgcgat atgtcagcat tctggcaatt gcgtacgtgg caacggcaag
     2821 ttatttaatc tcaaacgaaa gccgtggatc atgccggatg aagtcgacgt cgccactgtg
     2881 gttaaagtga ttgatacgtg cccgagcggc gcgctgaaat accgtcataa ataagcgagg
     2941 gtaaaatgga aatacgcgaa ggccacaata aattttacat taatgacgaa caaggcaagc
     3001 aaatcgctga aattgtcttt gtgccgactg gagagaattt agcgattatc gaacataccg
     3061 atgtcgatga aagcctgaaa tggcagggga ttggtaaaca gctggttgca aaagtcgtgg
     3121 aaaaaatgcg tcgggaaaaa cgaaaaatta tcccactatg cccatttgcg aaacacgaat
     3181 ttgataaaac gcgggagtat gatgatattc gcagttgatg ggagagtaca gagtcacgat
     3241 atttttcatg ctctccgcgg agtgatgcag gagagcatct gaagggtagg gggatgcaca
     3301 aagaatgggc agagagcgcg tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa
     3361 agaatatctc ctatatgaga atcatcaatc ggggttatca agttttgcgt ccccagagca
     3421 ttcaatattg ataggagtca tattatggaa ggtaaaaaca agttc
//
LOCUS       DAZRUF010000313         3437 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZRUF010000313 DAZRUF010000000
VERSION     DAZRUF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.316"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(64..324)
                     /gene="yfhL"
                     /locus_tag="ACHXOL_004003"
     CDS             complement(64..324)
                     /gene="yfhL"
                     /locus_tag="ACHXOL_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR6810843.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            539..1729
                     /locus_tag="ACHXOL_004004"
                     /pseudo
     CDS             539..1729
                     /locus_tag="ACHXOL_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1914..3101)
                     /locus_tag="ACHXOL_004005"
     CDS             complement(1914..3101)
                     /locus_tag="ACHXOL_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR6810844.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(3101..>3437)
                     /locus_tag="ACHXOL_004006"
     CDS             complement(3101..>3437)
                     /locus_tag="ACHXOL_004006"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810845.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT          866 a          928 c          806 g          837 t
ORIGIN      
        1 taaatataat ggcgttaagg cttcccagta atataattaa tactctactt ccagagtaga
       61 atattaaatt ttatccgcgt ggtgcatcag cacaaattta tcccacaact gttcttctgt
      121 ctcgacatgc gccggatctt tcacaatagt attggggatc gggcacacct tctggcaggt
      181 tggtgtctcg tagtgcccta cgcattcggt acacttatcg ctgttaatct cgtagatatg
      241 atctcccatt gaaatcgcct cattcgggca ttcgggttca cacatatcac aattaataca
      301 gcgtttagta attagtaaag acatttcaat ggattaccgt taaatcattt taaaatcagt
      361 aagttgtatc gagtttgtat gctttactgt cattaactta ctgtatgttg atccagtgta
      421 tttaaccttg ataaactcag tccagcaaca caaaaccgca acacattgca ttttgtcccg
      481 tagaaaagac ttgtatgtgt gagcttgttt tctgcgccta cgcagataag gattgagaat
      541 gccgcgcact gtaacacata atccggatag ccccaataat gacgatgttt tagccgcatc
      601 tgaaaaatgg gacgcctgta aaccccccta taccagcgca cacatgaaaa tctgtgttgc
      661 tgccgccaaa atcatcctcg ctgcttccgg cgtggctcgc cgttccaaat acgaaaaaga
      721 gaactatctc cgtatcgatt tcagcaaagc cggtaaggtt acattttacg ccgagtttcc
      781 aaaaaagatg ggcctcaaag gtaaaaagct cggcgagtgg ccggagctcg ctatccagct
      841 ggcgcgcgaa aaagcactag gtatggctga cggtggactg cgggcagagt ccgtacatgc
      901 agcgctggaa atgtaccggg atgacctcaa agccaaagta gcccggcaga agctgagccc
      961 ggacagtttc acaacctacg gggtgcgtat cgaccggatt aaagcaacgt tcggcgagcg
     1021 tgaggtgttc agcgacgtaa catacaatcg gctggtggaa gtgctggacg agtggatcgc
     1081 cactcgctcg aacaataacg ccctggagtt gtttgccgag ctccgtcggt tctggaagtt
     1141 ctgcgcacct actctttgca acggccgcaa tgttgccgcc agtctgccag atgattatgt
     1201 ttcctcccgc gtacagaaac ctacccccac acggcttttt accgatattg aatcaatcgc
     1261 ccgactctgg ctcaatgttg ctgcctgcac ctctgtacac cagaagaatg ctgttcgctt
     1321 catgatcatc actggtgttc gtccgattaa tgtccataac ctgcgctggg actacgttta
     1381 cgaggaggct ggtgaaattg tttatccgga aggggttatc ggcatgcgag gggctatgaa
     1441 aacacaaaag gctttccgcc tgccgataac gcctgagatc cggcggatta tcgacgagca
     1501 gaaagcctgg cgtgattcag ttcctgagtg caacagggat tatgtatttt tgcagccacg
     1561 tgatccaatg cagccatttt caaaacgatc actggataag ctggtgaaaa catacagccc
     1621 ggacggggct gtaaaaggaa taaaacatga tgggactgtt aaagggaaag acggtgcatt
     1681 taatacgatg tgccgtaaat tccttaagag caatgttatt gccttgatcg ataggaatta
     1741 aaaccccaaa aagattaaaa aaacaccaca aaacggatgt ttcttcaaca ccacttttgc
     1801 tcatatgaac ggaaccgacg attaaactgg atggctctga ttgattcagg gtatgaatgg
     1861 cggttttttg ctccgtttcc ctcaaaatgg acgcaacttt ccctctgcgg ctctcagccg
     1921 caccaccgca tccgggccag cggctcatgc atcaggacct gctctgccag acggtagccc
     1981 cgcttcagcc ccgtaaaacg catctgactc ccgcacagca cgcacttcag cgggtcaacc
     2041 ttcagtaacc tctgatacat ccctctccag gtgatttgca tcgccgtttt tctcactgtc
     2101 tccgttatga tgtacaccac ttcttccagt aaccgccgtt tcgccggact caaaaaaccg
     2161 tagtacctca ccatacggaa ccccttatcc gccacatgcc aggagaacct ttccatgaac
     2221 tcatctccac tcatcaacag gtattcttcc cgttttgttc ggtgactgtt gtaacgcaga
     2281 ccgatttcat cctgaccggc ataatgctcc agacgactca tcggcaccgg tggctttttc
     2341 aggtaagagc caaagtacac cgccacatgg ggggcattat ccatcacccg ggatacgttg
     2401 acattccagc cacggcggta atgcgtgtcc aggaagcgat tccattcccg tttactgctt
     2461 ccttctgctg ccagcgcatc cggcatcacc aggtcagggt atttccgtga cagcaaccgt
     2521 gttatccggt agcgccacat gctcatcacc ttacgggcgt aaaaatgaag atttttccag
     2581 gtgtggcccg acgtcacacc accggcagtt gtcgataaat ggatatgcgg atgccactgc
     2641 tggtcacgcc cccatgtgtg gatcaccgtg aatatccccg gctccacatc tgcctgatgg
     2701 cagatttcca gtatcacatc cgctgcaatg cggctcatct ctgtcagtaa ccaccggttg
     2761 tggaacacca gggaccagta ctggcaggga agtgtgaaca caatatgctg ccacgggcag
     2821 tcggggacca ggctcagcag atactgtatc cactgtgcgc cagccttcac cccgcagtgc
     2881 gggcaggagc ggcttttaca ccggaagcag accttttttg tatggcaaca gtccggtgat
     2941 gaacagcacc actgtgtata ccccatcagt gtggtcccgc acgccatgat tttggtcacc
     3001 gactcaatca ccaccggacg tactgccctt tccggctgct tctccagcca gttaagccag
     3061 tggtttccct gctgaaagat atcggcaaaa cggggaagca tcagaagggc ggggcgactc
     3121 cgtccggcca gtgaaccgtg ccacactccg ggcagtacat accgccggcg ctgataccgg
     3181 aaagaatggt cgcaaattcc cgctccgtgc agcgggcgat ttccggatac ccttcgtcat
     3241 caacacgtac aaaccagaag accagctttt tgtttcccgc atccacaaag aacggaatat
     3301 tcaggtctgc gcagcattca acggcatcgt caaaactatc aaagcgcaga acttctgcgt
     3361 cttcttcgtc aaaaaaatca tcttcgtgaa gcttcacgac atagcgggga agtttgcttc
     3421 tttgagaggc gggttta
//
LOCUS       DAZRUF010000314         3382 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZRUF010000314 DAZRUF010000000
VERSION     DAZRUF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.317"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..1805
                     /gene="fimD"
                     /locus_tag="ACHXOL_004007"
                     /pseudo
     CDS             1..1805
                     /gene="fimD"
                     /locus_tag="ACHXOL_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1818..2825
                     /gene="fimH"
                     /locus_tag="ACHXOL_004008"
     CDS             1818..2825
                     /gene="fimH"
                     /locus_tag="ACHXOL_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR6810846.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2836..3351
                     /gene="sfmF"
                     /locus_tag="ACHXOL_004009"
     CDS             2836..3351
                     /gene="sfmF"
                     /locus_tag="ACHXOL_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR6810847.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
BASE COUNT          839 a          757 c          871 g          915 t
ORIGIN      
        1 cgtggtgtac ggctttattc ttccgataat atgtatccag atagccagca agggtttgcc
       61 ccaacggtac gtgggattgc ccgtacggcg gcccagctaa cgattcggca aaatggtttt
      121 attatctatc aaagctatgt ttcccccggt gcttttgaaa ttactgattt gcacccgaca
      181 tcttcaaatg gcgatctgga tgtcaccatc gacgagcgcg atggcagaat tacacaattc
      241 cgtattcaac agtgccgatt ttacaacgcg aagggcgttt caaatttgac ctgacggcgg
      301 gcgattttcg tagcggtaat agtcagcaat catcaccttt cttttttcag ggcacggcac
      361 tcggtggttt accacaggaa tttactgcct acggcgggac gcaattatct gccaattaca
      421 ccgccttttt attagggctg gggcgcaacc tcgggaactg gggcgcagtg tcgctggatg
      481 tgacccatgc gcgcagccag ttagccgacg acagtcgtca tgagggggat tccattcgct
      541 tcctctatgc gaaatcgatg tacaccttcg gcaccaattt tcagttaatg ggttaccgct
      601 attcgacaca aggtttttat acccttgatg atgttgcgta tcgtcgaatg gaggggtacg
      661 aatatgatta cgattacgac ggtgagcatc gcgatgaacc gataatcgtg aattaccaca
      721 atttacgctt tagccgcaaa gaccgtttgc agttaaatat ttcacaatca cttaatgact
      781 ttggctcgct ttatatctct ggtacccatc aaaaatactg gaatacttcg gattcagata
      841 cctggtatca ggtggggtat accagcagct gggttggcat cagttattca ctctcatttt
      901 cgtggaatga atctgtaggg atccccgata acgaacatat tgtcggactt aatgtttcag
      961 tgcctttcaa tgttttgacc aaacgtcgct acacccggga aaatgcgctc gaccgcgctt
     1021 atgcctcctt gaacgctaac cgtaacagca acgggcaaaa tagctggctg gcaggcgttg
     1081 gtgggacctt actggaaggc cacaacctga gttatcacgt aagccagggc gatacctcga
     1141 ataatgggta cacgggcagc gccacggcaa actggcaggc cacttacggt acgctggggg
     1201 tcgggtataa ctacgaccgc gatcaacatg acgttaactg gcagctgtct ggcggtgtgg
     1261 tcgggcatga aaatggcata acactgagcc agcctttagg ggataccaat gttttgatta
     1321 aagcgcctgg cgcaggcggt gtacgcattg aaaatcaaac tggcatttta accgactggc
     1381 gcggctatgc ggtgatgccg tatgccacgg tttatcggta taaccgtatc gcgcttgata
     1441 ccaatacgat ggggaattcc atcgatgttg aaaaaaatat tagcagcgtt gtgccgacgc
     1501 aaggcgcgtt ggttcgtgcc aattttgata cccgcatagg cgtgcgggcg ctcattaccg
     1561 ttacccaggg cggaaaaccg gtgccgtttg gatcactggt acgggaaaac agtaccggaa
     1621 taaccagtat ggtgggtgat gacgggcaag tttatttaag tggtgcgcca ttgtctggtg
     1681 aattactggt tcagtgggga gacggcgcga actcacgctg cattgcgcac tatgtattgc
     1741 caaagcaaag cttacagcaa gccgtcactg ttatttcggc agtttgcaca catcctggct
     1801 cataaaggaa attatcaatg aagataatct gcagattatt attggcaatg gcatgtttgt
     1861 gtctggcaaa catatcctgg gctactgttt gtgcaaatag tactggcgta gcagaagatg
     1921 aacactatga tctctcaaat gtctttaata gcaccaataa ccagccaggg cagattgttg
     1981 ttttaccgga aaaatccggc tgggtaggtg tctcagcaat ttgtccaccc ggcacgctgg
     2041 tgaattatac ataccgtagt tatgtcacta actttattgt tcaggaaact atcgataatt
     2101 ataaatatat gcaattaaat gattatctat taggtgcgat gagtctggtt gatagtgtga
     2161 tggatattca gttccccccg caaaattata ttcggatggg aacagatcct aacgtttcgc
     2221 aaaaccttcc gttcggggtg atggattctc gtttgatatt tcgtttaaag gttattcgtc
     2281 cctttattaa catggtggag atccccagac aggtgatgtt taccgtgtat gtgacatcaa
     2341 cgccttacga tccgttggtt acacctgttt ataccattag ttttggtggc cgggttgaag
     2401 tgccgcaaaa ctgcgaatta aatgccgggc agattgttga atttgatttt ggtgatatcg
     2461 gcgcatcgtt atttagtgcg gcagggccgg ggaatcgacc agctggcgtc atgccgcaaa
     2521 ccaagagcat tgcgatcaaa tgtacgaatg ttgctgcgca ggcttattta acaatgcgtc
     2581 tggaagccag tgccgtttct ggtcaggcga tggtgtcgga caatcaggat ttaggtttta
     2641 ttgtcgccga tcagaacgat acgccgatca cgcctaacga tctcaatagc gttattcctt
     2701 tccgtctgga tgcagctgcg gcagccaatg tcacacttcg cgcctggcct atcagtatta
     2761 ccggtcaaaa accgaccgaa gggccgttta gcgcgctggg gtatttacgc gtcgattatc
     2821 aatgaggtac ggagaatgag aagagtactc tttagctgtt tctgcgggtt actgtggagt
     2881 tccagtggat gggcagctga ccctttggga acgattaata tcaatttgca cggtaacgtt
     2941 gttgatttct cctgtaccgt aaacactgcg gatattgata agacggtaga tttaggcaga
     3001 tggcctacga cacaattact gaacgctggc gataccacgg cactcgtccc ttttagcctg
     3061 cggctggagg gatgtcctcc gggttcagtt gcgattttat ttacgggaac gccggcatcc
     3121 gataccaacc tgctggctct ggatgatccc gcaatggcac aaaccgtcgc catcgaatta
     3181 cgtaatagcg atcgctcccg gctcgcactg ggggaggcga gcccgactga ggaagtagat
     3241 gcaaatggca atgtcacact aaactttttt gccaattatc gagcgttagc cagcggtgtt
     3301 cagccaggtg tggagaaagc ggatgcgata tttatgatca attataatta atattatatt
     3361 aattcgtata atttggcgta gt
//
LOCUS       DAZRUF010000315         3378 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZRUF010000315 DAZRUF010000000
VERSION     DAZRUF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.318"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            13..128
                     /gene="rrf"
                     /locus_tag="ACHXOL_004010"
     rRNA            13..128
                     /gene="rrf"
                     /locus_tag="ACHXOL_004010"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(232..759)
                     /gene="mobB"
                     /locus_tag="ACHXOL_004011"
     CDS             complement(232..759)
                     /gene="mobB"
                     /locus_tag="ACHXOL_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR6810848.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(741..1325)
                     /gene="mobA"
                     /locus_tag="ACHXOL_004012"
     CDS             complement(741..1325)
                     /gene="mobA"
                     /locus_tag="ACHXOL_004012"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR6810849.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1395..1664
                     /locus_tag="ACHXOL_004013"
     CDS             1395..1664
                     /locus_tag="ACHXOL_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR6810850.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1741..2727
                     /gene="srkA"
                     /locus_tag="ACHXOL_004014"
     CDS             1741..2727
                     /gene="srkA"
                     /locus_tag="ACHXOL_004014"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR6810851.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2744..3370
                     /gene="dsbA"
                     /locus_tag="ACHXOL_004015"
     CDS             2744..3370
                     /gene="dsbA"
                     /locus_tag="ACHXOL_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR6810852.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          861 a          820 c          830 g          867 t
ORIGIN      
        1 aaacagaatt tgcctggcgg ccgtagcgcg gtggtcccac ctgaccccat gccgaactca
       61 gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac
      121 tgccaggcat caaattaagc gaaaggccat cctgacggat ggcctttttg cattggcgca
      181 gaaaaaaatg ccggatgcga cactggcgcg tcttatccag cctactcttg cttatccgtt
      241 ctgcttttgc atccactcca ccacaaaatc agccagcccc tcaacatcat taatatccag
      301 taatgcgaca tcaagattaa gcggcacatc actggctaca gcaataacat gcctgtctat
      361 cactaattct tcaggtcgat gtccggctcc atcgcgaaac agcacaatct ttgcgatctc
      421 ttcatgctta aacccttcga ccagaatcaa atccagcttt gaggtatcca ttcgacttgc
      481 gagaaactgt agatccagct cttcttcgtc tggtttttcc gtcatcaagg cccatcgttg
      541 ctggctggca acgatggttt gtgccgcgcc agccttgcgc agctcatagc tatctttgcc
      601 tggcttatca acatccatat catggtgcgt atgcttaatc agccctggac ggatccctct
      661 ggcgcataat gccgggatca gttttttcaa aagcgtagtt tttccggtac cactccacgc
      721 ggcaaaggcg agtaacggta tcatcgtttt tcctgccatc gggcaagctc ctctggcgta
      781 ttcacgttaa caaatgcatc tttatgatcg ctgaaatcaa ccgcatggcc gccagccaga
      841 cgcataaatg ccattacccg gcgttctcct gcttgcagat attctagtaa taaaggctca
      901 atagcacggt ttaccagagc aatagtcggg tgatcgcgtt caccgtcatg gacccacacg
      961 acaggcgcat ctttgcgctg atgattaagc cgggaagcta aaacatgggg aatgtaaggc
     1021 gtatcgcacg gacaaaacaa aaaccactca ccagcttcct gctgcattac tgaaagcatt
     1081 cctgccagag ggcctgggta atccgccagt gaatcttcaa tcactttcag accgcttgcc
     1141 tgatagattt cctgatgacg attggcatta accacgacgt gagagagctg cgtcataagc
     1201 gcgtcagcga catgttgcca taatggtttg ccgtttaatt caagcaatcc tttatctacg
     1261 ccgcccattc gtctggcttt accgcctgcc agcacaacgc ctgttatcgt cgtcatcaga
     1321 ttcaccgata tcgcctcttt tattgtggga ttgaccctgc taacgtgtct gtctcaagag
     1381 taaggagcat cactatgaaa tgtaaacgtc tgaatgaagt tattgaactc ctccagccag
     1441 cctggcaaaa agagccagac cttaacctgt tgcaattttt gcagaaactg gcgaaagagt
     1501 caggttttga cggcgaactg gcggatttga cggatgacat tctgatctat cacctgaaaa
     1561 tgcgtgattc cgcgaaagat gcggtgatcc cgggtttgca gaaagattat gaagaagact
     1621 tcaaaacggc gctgttacgc gctcgcggcg taattaaaga gtaaaagctt gtaagcggcg
     1681 ccaccaaaat catcgtgaaa tgatatcctt catcattcgt aatgttttcc ggatgatggg
     1741 atgaacaaca gcgcttttac tttccagaca ctacacccgg ataccatcat ggacgctctg
     1801 tttgagcaag ggatccgggt ggattccggt cttaccccgc ttaacagcta tgaaaaccgt
     1861 gtctatcaat ttcaggacga agagcgtcga cgttttgtcg tcaaatttta tcgccctgaa
     1921 cgttggacag ccgatcaaat cctcgaagaa catcaatttg cgttgcagct ggtaaatgat
     1981 gaagttccgg tcgcagcacc tgtggccttt aacggtcaga ctttattgaa tcatcaggga
     2041 ttttatttcg ctgtttttcc aagcgtcggt ggtcgccagt tcgaagctga taatatcgat
     2101 cagacggaag cggttgggcg ttatttaggg cgtatgcacc agacggggca caaacagctt
     2161 tttatccatc gcccgaccat cggtctgaat gaatatctca ttgagccacg caagctgttt
     2221 gaggacgcta cactgatacc ttccgggttg aaagcggcat tcctgaaagc gacagatgag
     2281 ctgatcgccg ccgttacagc acactggcgg gaagatttca ccgttctgcg gctacatgga
     2341 gactgccacg ccgggaatat tctctggcgc gatggtccaa tgtttgttga tctggatgat
     2401 gcacgtaatg gtccagctat tcaggatttg tggatgttgc tcaatggcga taaagccgag
     2461 cagcggatgc aactggaaac tattattgaa gcttatgaag aatttggcga gttcgacacc
     2521 gctgaaatcg gactgattga acctttacgc gccatgcgtt tggtttatta tcttgcctgg
     2581 ctaatgcggc gttgggctga tcccgcgttc ccgaaaaatt tcccgtggtt aaccggggaa
     2641 gattactggc tgcgacaaac ggcgactttt atagaacagg caaaagttct acaagaaccc
     2701 cctttacaat taacacctat gtattaatcg gagagagtag atcatgaaaa agatttggct
     2761 ggcgctggct ggtttagttt tagcgtttag cgcatcggcg gcgcagtatg aagatggtaa
     2821 acagtacact accctggaaa aaccggtagc tggcgcgccg caagtgctgg agtttttctc
     2881 tttcttctgt ccgcactgct atcagtttga agaagttctg catatttctg ataatgtgaa
     2941 gaaaaaactg ccggaaggcg tgaagatgac taaataccac gtcaacttca tgggtggtga
     3001 cctgggcaaa gatctgactc aggcatgggc tgtggcgatg gcgctgggcg tggaagacaa
     3061 agtcactgtt ccgctgtttg aaggcgtaca gaaaacccag accattcgtt cagcatctga
     3121 tatccgcgat gtatttatca acgcaggtat taaaggtgaa gagtacgacg cggcgtggaa
     3181 cagcttcgtg gtcaaatctc tggtcgctca gcaggaaaaa gctgcagctg acgtgcaatt
     3241 gcgtggcgtt ccggcgatgt ttgttaacgg taaatatcag ctgaatccgc agggtatgga
     3301 taccagcaat atggatgttt ttgttcagca gtatgctgat acagtgaaat atctgtccga
     3361 gaaaaaataa ttcaatgt
//
LOCUS       DAZRUF010000316         3322 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZRUF010000316 DAZRUF010000000
VERSION     DAZRUF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.319"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="ACHXOL_004016"
     CDS             complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="ACHXOL_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR6810853.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(366..1085)
                     /gene="fliA"
                     /locus_tag="ACHXOL_004017"
     CDS             complement(366..1085)
                     /gene="fliA"
                     /locus_tag="ACHXOL_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFR6810854.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1250..2824)
                     /locus_tag="ACHXOL_004018"
     CDS             complement(1250..2824)
                     /locus_tag="ACHXOL_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFR6810855.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3079..3322
                     /locus_tag="ACHXOL_004019"
                     /pseudo
     CDS             3079..3322
                     /locus_tag="ACHXOL_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          852 a          789 c          713 g          968 t
ORIGIN      
        1 accgtacctg ggctcatttc agtttgctca aaaagaaatt gcttaaagcc gataatatcg
       61 aaaaaatcac tctgcgtttt gcagtgtaaa tcaccacaaa atcggcacaa tgccgcccat
      121 gatttttgtt ccacttgcca gccattttca tcaagcagca tgtccaggcg ggaaatctcg
      181 attttattca ctattttgcc gtcgcgaacg agggtaatgc ggtcgagtaa tttacggcaa
      241 tgcgcgcaat gagtctggct gtgtttaaag tctttaagat agcggcttaa tggccgtctt
      301 ttcaggtgct gcaccatcat taagaactcc tggtagtcaa aattaaagtg cggcatttac
      361 tgacgttata acttacccag tttagtgcgt aaccgtttaa tggcctggct gtgtaactga
      421 ctgacccgcg attccccgac ctccagcacc gcgccaatct ctttgagatt cagctcttcc
      481 tggtaataga gggttaatac cagtttttcg cgctccggca acgtttcgat ggcttccatc
      541 acccgctggc gcagattact gtccagcagt tgttgtagcg ggttttctcg ctgatgatca
      601 tcagtaacca gttcgatgct atcgccgtgc tcttcgcgcc actcatcgta ggagaagagc
      661 tggctgttat tggtgtcgag caacatttgg cgataatcgg caatatcgat ccctaaacgt
      721 tccgctacct cagtttccgt ggcgttgcgg ccaagttcct gctccagttg ccctattgcc
      781 tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca cccagtcacg gctgcgaagt
      841 tcatccagca tagcgccacg gatacgctgc actgcgtaag ttgtaaatgc cgttccttgt
      901 agggcgtcat agcgttcgac ggcattaagt aacccaatgc cgcccgcctg tagcagatcg
      961 tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg acgcaccagc
     1021 gggacataac gctgccacag cgagtgttta tccattacac cttcagcggt atagagtgaa
     1081 ttcacgataa acagccctgc gttatatgag ttatcggcat gattatccgt ttctgcaggt
     1141 tttttaatcg gacgattagt gggtgaaatg aggggttatt taggggttac cggtaaattg
     1201 cggacagaaa aaaccccgcc ggtggcgggg tttgagcgat aagtgtaaat taacgcagca
     1261 gagacagtac gttctgcgtg gtctggttag cctgtgccag aacagaggta cccgcttgtt
     1321 gcaggatctg cgcacgagac atgttagaca cttcggttgc gtagtcagca tcttctatac
     1381 ggctacgggc agaagacagg tcgtttacgg tgttgccaag gttggtgatg gcagagtcga
     1441 aacggttttg tactgcaccc aaagaactgc gcaacttatc aacttttgcc aaagcttcgt
     1501 ctaatttgga taatggattt gtggttgcta aggctttcaa atctgaggca ctgagttcag
     1561 cacctttggt tgccgtctta ttcgtgatat tggctgtata aaccttatca tcttcacgca
     1621 taattgcctg tgcagaacca tcatttttta ctgcccattc aactgcctta ccattaggat
     1681 cagtaataac agacgtgtta cctgtagcgg catctacatt tactgcaatg taggtgccgt
     1741 ctgatgtttt agcagcaaat gaattaggta caggattccc ttgggtatct tgtacttggt
     1801 ggacatctag gttttttccc ccaaaataaa cagacattgg tgcatcatta taagctaatg
     1861 ctttacccat tttacccgca gctaaatcaa ctgatgtggc agaaccatta tcttcaacaa
     1921 atgcagttac acgaccacca aagtttgcta actgaacatc cttaccaccc acctgaactt
     1981 tttcataacc tgtagagttt tgtttcaata atgtaaaacc gttcgtgttg gtattattat
     2041 cttgaccaac aaaataagtc tgcccttcca cctgtacttg ataaactttt gttcctgcag
     2101 taccggtcgc atcagttgta tcctttgtat cttgtacatt accggtacca aaataagttt
     2161 caacatcagc atttttataa ttgctaccga atgctttagc acctcccaca ctaccatcag
     2221 ttgcatttac cttagttaag tcaggagcgg cttcgctctt aagtgcaacc gcagaggaca
     2281 ttggaacttt tcctagtgct acgctaaagt tatcaagccc caaggtttta gcatcaattt
     2341 tatcaagggc aatctcaatg gtttcaccat cgttcgcacc tacttgaatt ttcatagtct
     2401 gatcagaagc cagaactttt acaccattaa attgtgtctg gttagaaaca cgatcaattt
     2461 cactaaggcg ttgtgtaatt tcatcctgaa ttgaattcag gtcggagtca gagtttgtac
     2521 cattagtggc ctgtactgac aattcacgaa tacgctgtaa gttattgttg atttcagaaa
     2581 gcgcaccttc agtggtctgc gcaacagaaa taccatcatt cgcgttacgg gaagcctgag
     2641 tcagaccttt aatatttgct gtaaaacggt tagcaatcgc ctgacctgct gcgtcatctt
     2701 tagcactgtt aatacgcagg ccagaagaga gacgttcaat ggcggagctc agagaagact
     2761 gagatttgtt caggttattc tgggtcaaca gcgacaggct gtttgtatta atgacttgtg
     2821 ccatgatctt ttccttatca attacaactt gatgttattg ggctgttgcc cacggtttct
     2881 caccgtaccc cttgtatcgg cacctgaatt tcgaacttta gaaaatttta gtttctctcc
     2941 tgttttttct taacggctga aatatccgta atatacaact ctactctgcg catcatcttc
     3001 gtgaatcaac tattaaactt tgcgcacaaa ttgccgataa cccgcttatc tactgttttc
     3061 aatcaaaagg aattaggtat ggcttcattt acatctcttg gcgttggttc gaatctgccg
     3121 ctggatacat tgctaaacaa cctgaccatc gctgaaaaaa aacgcttaaa cccaattact
     3181 cagcaacaga gtgacaatac cgcacgtctg acagcctatg gcacattaaa aatgtgcatt
     3241 agaaaaattc cagactgcaa atactgcgtt aaataaagcc gatctgttca aaagcactac
     3301 cgttaccagt agcaatgaag ac
//
LOCUS       DAZRUF010000317         3244 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZRUF010000317 DAZRUF010000000
VERSION     DAZRUF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.320"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            248..1627
                     /gene="ygjG"
                     /locus_tag="ACHXOL_004020"
     CDS             248..1627
                     /gene="ygjG"
                     /locus_tag="ACHXOL_004020"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR6810856.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(1669..2001)
                     /locus_tag="ACHXOL_004021"
     CDS             complement(1669..2001)
                     /locus_tag="ACHXOL_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR6810857.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            2220..3203
                     /gene="ebgR"
                     /locus_tag="ACHXOL_004022"
     CDS             2220..3203
                     /gene="ebgR"
                     /locus_tag="ACHXOL_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR6810858.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT          817 a          852 c          821 g          754 t
ORIGIN      
        1 ttaattatct tgcccaaaaa tcaggcaatt attgccctga aaacgtgcat ttgcgcagca
       61 atcatcaaat ccatacccga caaaaaccgt gcaaaataac aacaaatgtt aacagatagc
      121 attaaatatt gcacaaatga taaccgaatt tgtgtttatc ccgattttcg cgatcgcagc
      181 cggagtggcg caatccctgc aatacttaaa tcggtatcat gtgatacgcg agcctccgga
      241 gcatattttg aacaggttac cttcgagcgc atcggcttta gcgtgcagcg cccacgccct
      301 gaatctcatt gagaagcgaa cgctggatca tgaggagatg aaagcactta accgagaggt
      361 gattgaatac ttcaaagagc atgtcaatcc ggggttttta gagtatcgca aatctgttac
      421 cgccggcggg gattacggag ccgtagagtg gcaagcggga agtttaaata cgcttgtcga
      481 cacccaggga caggagttta tcgactgcct gggaggtttt ggaattttca acgtggggca
      541 ccgtaatcca gttgtggttt ccgccgtaca gaatcaactt gcgaaacaac cgctgcacag
      601 ccaggagctg ctcgatccgt tacgggcgat gttggcgaaa acccttgctg cgctaacgcc
      661 cggtaaactg aaatacagct tcttctgtaa tagcggcacc gagtccgttg aagcagcgct
      721 gaagctggcg aaagcttacc agtcaccgcg cggcaagttt acttttattg ccaccagcgg
      781 cgcgttccac ggtaaatcac ttggcgcgct gtcggccacg gcgaaatcga ccttccgcaa
      841 accgtttatg ccgttactgc cgggcttccg tcatgtgccg tttggcaata tcgaagccat
      901 gcgcacggct cttaacgagt gcaaaaaaac cggtgatgat gtggctgcgg tgatcctcga
      961 accgattcag ggtgaaggtg gcgtaattct gccgccgccg ggctatctca ccgccgtacg
     1021 taagctatgc gatgagttcg gcgcactgat gatcctcgat gaagtacaaa cgggcatggg
     1081 gcgcacgggc aagatgttcg cctgcgagca tgagaacgta cagccggata tcctctgcct
     1141 tgccaaagcg ctcggcggcg gcgtgatgcc gattggcgcg accatcgcca ctgaagaggt
     1201 gttttcagtt ctgttcgaca acccattcct gcataccacc acctttggcg gcaacccgct
     1261 ggcctgtgcg gcggcgctgg cgaccatcaa tgtgttgctg gagcagaact taccggctca
     1321 ggctgatcaa aaaggcgata tgttgctgga cggtttccgt caactggcgc gggaatatcc
     1381 cgatctggta caggaagcgc gtggtaaagg gatgttgatg gcgattgagt ttgttgataa
     1441 cgaaatcggc tataactttg ccagcgagat gttccgccag cgcgtactgg tggccggaac
     1501 gctcaataac gccaaaacga tccgcattga accgccactg acactgacca ttgaacagtg
     1561 tgaactggtg atcaaagcgg cgcgtaaggc gctggcggcc atgcgagtaa gtgtcgaaga
     1621 agcgtaatat caaatcggat ggcgatacga cgtcgccatc cgatcttttt atacgacgcg
     1681 tacgcccgca ggcatcatcc gctccggcgt taacagcacg ctttcactgc cgtcatcggt
     1741 ttccgcacac aacagcatgc attccgacgt ttcaccacgc attttcgctt tttgcagatt
     1801 gcataagacc accaccgttt ttcccatcag ctcctcttcg ctgtagtacg gcaccaggct
     1861 ggtcacggtt tgcagtgttt tttcccccac atcaacctgt acgatgtaca gcttgtcggc
     1921 gttttcatgg cgtttcactt ccacaatctt tccgacgcgc atttccagac gtgcaaaatc
     1981 agcgtaagcc acggtttcca ttgcttcctc ccttcaagta aaattttact aaactatagc
     2041 aaaatttttt ctcaatcctg taggctaaaa atggagaatg caggcgtgat cacattcgta
     2101 agccgctgtg ttaccgttac agcgtcaaag aaacgcgctt tatttactga aaacaggtga
     2161 cccgataagc acttcctcta caatgggggc gcacatcagg gaaagtaaaa aaggtaaaca
     2221 tggcaacatt aaaagacatc gcaatcgaag ctggcgtatc cctggcgaca gtatccaggg
     2281 tcttaaatga cgatccgaca ttgaatgtga aagaagagac gaaacatcgc attctcgaga
     2341 tcgccgaaaa gctggagtac aagaccagta gcgcccgtaa actacagaca ggcgcagtca
     2401 accaacacca tattctggct atctacagct accagcagga gctggagatc aacgatcctt
     2461 actatctggc gatccgccac ggcattgaaa ctcagtgcga aaagctggcg atcgaactga
     2521 ccaactgtta tgaacacaac ggattaccag acattaaaaa cgtcaccggt attttaattg
     2581 tcggcaaacc cacgcccgcc ctgcgcgccg ctgccagcgc gttgaccgac aatatctgtt
     2641 ttatcgactt tcacgaaccc ggcagcggtt acgatgcggt ggatatcgat ctggcacgca
     2701 tcagtaaaga aatcatcgac ttctatatca accagggcgt taatcgtatt ggttttattg
     2761 gcggtgaaga tgagcctggc aaggcggata ttcgtgaggt cgcctttgcg gaatatggcc
     2821 gactgaaaca agtggtacgc gaagaggata tctggcgcgg cggtttttcc agttcgtcgg
     2881 gttatgaact ggcaaaacaa atgctggcgc gggaagacta tccgaaggca ctgtttgttg
     2941 cttccgattc cattgctatc ggcgtactgc gggcaattca tgaacgtggc ctgaacatcc
     3001 cacaggatat ttcgcttttc agcgttaacg atatccccac cgcgcgattt acctttccgc
     3061 cgctctccac cgtgcgcatc cattccgaga tgatgggaag tcagggcgtt aacctggtgt
     3121 atgaaaaagc ccgcgatggt cgcgcgctgc cgctgctagt cttcgttccc agcaaattaa
     3181 aactgcgcgg cacgacccgt taaatcccct tacacactgt ccggcaatca tttttgccgg
     3241 acag
//
LOCUS       DAZRUF010000318         3216 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZRUF010000318 DAZRUF010000000
VERSION     DAZRUF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.321"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..495
                     /locus_tag="ACHXOL_004023"
     CDS             <1..495
                     /locus_tag="ACHXOL_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR6810859.1"
                     /translation="SRAPFIITPPLFRIEAGDDSSLRIIKTADNLPENKESLFYINVR
                     AIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRSDHSLNIYNP
                     TEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYGGSSGTET
                     RPLQ"
     gene            525..3041
                     /locus_tag="ACHXOL_004024"
     CDS             525..3041
                     /locus_tag="ACHXOL_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR6810860.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3052..>3216
                     /locus_tag="ACHXOL_004025"
     CDS             3052..>3216
                     /locus_tag="ACHXOL_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6810861.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          938 a          771 c          731 g          776 t
ORIGIN      
        1 agccgtgccc catttattat aaccccaccg ctatttcgta ttgaagctgg cgatgactca
       61 tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc gctgttctac
      121 attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc taacgagttg
      181 acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc ccgcacacct gaagggacgg
      241 gtaaacgatg cgtggacatc actggaattt aaacgtagtg accattcact caatatatat
      301 aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac cgatctcaca
      361 agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc tgcatctggc
      421 ggaaagaacg tgaaatgggc tgcaatcaat gattatggcg gcagttccgg gacagaaact
      481 cgtccactgc aataacaaat ataaaaaaca caggtcatca gggaatgcca caacgacacc
      541 accagggaca taaacgcaca ccgaaacagt tggcgctcat catcaaacgt tgtttgccga
      601 tggtgctcac tggcagcggc atgctttgca ctaccgctaa cgccgaagag tattatttcg
      661 accccattat gctggaaact acaaaaagtg gtatgcaaac aaccgatctg tcacgttttt
      721 caaaaaaata cgcacaacta ccaggaactt atcaggttga tatctggctg aataaaaaga
      781 aggtttcaca gaaaaaaatt acatttaccg ccaatgcaga gcaacttctg cagccacagt
      841 ttacggtaga acaactacgt gagctgggta ttaaggtgga tgaaatcccg gcgctggctg
      901 aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat cattcccggt acagcagctg
      961 aatttgattt caatcatcag cgacttaatt tgagcattcc ccaaattgca ctgtaccgtg
     1021 atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg tataccaacg ctgtttacca
     1081 actactcgtt tacaggttct gataaccgtt accgccaggg caatcgtagc caacgacagt
     1141 acctgaatat gcaaaatggt gctaattttg gcccctggcg attacgcaac tattccacat
     1201 ggacacgcaa cgatcagaca tcaagctgga ataccatcag tagttattta caacgtgata
     1261 tcaaggcatt gaagtctcag ttgcttctgg gagaaagcgc caccagcggc agtatttttt
     1321 ccagctacac ctttactggc gttcaactcg cttccgacga taatatgttg ccaaacagcc
     1381 agcgcggatt tgccccaacg gtacgcggta tcgcaaacag tagtgcaatc gtgactatca
     1441 ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc gggtgccttt gaaattaacg
     1501 atctctaccc ctcttccaac agcggcgatt tagaagtcac gattgaagaa agtgacggta
     1561 cgcaacgtcg ctttatccag ccttattctt cattacccat gatgcagcga cctgggcatc
     1621 taaaatatag cgcgaccgct ggacgctatc gcgctgatgc aaacagtgat agcaaagaac
     1681 ccgaatttgc tgaagccacg gcaatatatg gtttgaataa tacttttacg ctgtatggtg
     1741 gcctgctcgg ttctgaagat tattatgcgc tggggatcgg tatcggcggc acacttggcg
     1801 cactgggcgc gttgtcgatg gatatcaaca gagctgacac ccaattcgat aaccagcact
     1861 cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga tatcccggaa accaacacca
     1921 atatcgctgt cagctactat cgctatacca acgatggcta ttttagtttt gatgaagcca
     1981 atacccgtaa ttggaactat aacagtcgcc aaaaaagtga aattcaattc aacatcagcc
     2041 agacaatatt tgatggggta agtctgtatg cctccggttc gcagcaagac tattggggca
     2101 ataacgataa aaacaggaat atctcagttg gggtttccgg tcagcaatgg ggaattggtt
     2161 acagcctgaa ttatcaatac agtcgctaca cggatcaaaa taatgaccgc gcactctctt
     2221 tgaatctcag tattccgtta gaacgctggt taccgcgtag ccgggtttcc tatcagatga
     2281 ccagccagaa agatcgccca acccaacatg aaatgcgtct taatggctca ctgctggatg
     2341 atggtcgcct gagctatagc ctggaacaaa gtctggatga cgataacaac cataacagta
     2401 gcctgaacgc cagttaccgt tcaccttatg gcaccttcag tgccggatac agctacggta
     2461 atgacagcag ccaatacaat tacggcgtta ccggcggcgt ggttatccat actcatggcg
     2521 tgacgctctc gcaatatctg ggcaacgctt ttgcgcttat cgatgctaat ggggcttctg
     2581 gcgtgaggat acaaaactat ccggggattg ctactgatcc ctttggctat gcagtggttc
     2641 cttatctcac gacttaccag gaaaaccgtc tctcggtaga tactacgcag ctgcccgata
     2701 acgtcgatct tgagcaaaca acacagtttg tggtgcccaa cagaggtgca atggtagcgg
     2761 cgcgtttcaa cgccaatatc ggttatcgcg tacttgttac agtcagcgat cgcaacggta
     2821 aaccgttgcc ctttggcgct cttgccagca acgatgagac ggggcaacaa agtatcgtcg
     2881 atgagggcgg catactatat ctctctggga tatcgagtaa atcacaaagc tggactgtac
     2941 gctggggaaa tcaggcagat caacaatgtc agtttgcttt tagtacaccg gattcagaac
     3001 caacaacctc tgtattacaa ggcacggcgc agtgccatta aggataaaaa aatgaaaaga
     3061 gcgcctctta taacaggact tttgttgata tccacatcct gcgcttatgc ctcctcagga
     3121 gggtgtggag ctgacagcac tagcggtgcg acaaattaca gcagtgtggt tgatgatgtt
     3181 acggtgaacc agacagataa cgtgacagga cgggag
//
LOCUS       DAZRUF010000319         3212 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZRUF010000319 DAZRUF010000000
VERSION     DAZRUF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.322"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            83..799
                     /gene="nanC"
                     /locus_tag="ACHXOL_004026"
     CDS             83..799
                     /gene="nanC"
                     /locus_tag="ACHXOL_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR6810862.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            819..1925
                     /gene="nanM"
                     /locus_tag="ACHXOL_004027"
     CDS             819..1925
                     /gene="nanM"
                     /locus_tag="ACHXOL_004027"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR6810863.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1990..2970
                     /locus_tag="ACHXOL_004028"
     CDS             1990..2970
                     /locus_tag="ACHXOL_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR6810864.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3080..3212
                     /locus_tag="ACHXOL_004029"
                     /pseudo
     CDS             3080..3212
                     /locus_tag="ACHXOL_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          946 a          680 c          758 g          828 t
ORIGIN      
        1 caaaaaatat tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt
       61 atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact
      121 gtgcttttcg tcaccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg
      181 tagcggaagc catgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg
      241 gtgggcaagc atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc
      301 cgcactcaat gatgttcagg ttgaagttaa ttacgcgatt aaacttgatg atcaatggac
      361 ggtgcgcccg ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt
      421 aaaactgtcc tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg
      481 gaaagcttac cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg
      541 ggatggatat gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct
      601 atacagcaaa cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc
      661 ctttgttcta caataccata tgacgcccga tattacgcca tacatagaat atgactacct
      721 tgaccgccag ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg
      781 tgtgtcattt aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc
      841 ttgctatcat gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc
      901 catttaaaag tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg
      961 caggtacagc atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag
     1021 ctgcattccc tggtggaccg agagatcaag caacctctgc atttattgat ggcaatctat
     1081 atgtgtttgg cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac
     1141 acaaatacaa ccccaaaacc aatagctggg ttaaattgat gtcgctcgcg ccgatgggca
     1201 tggcgggtca tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc
     1261 agaatatctt caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta
     1321 tagataaaat caacgcccac tattttgaca aaaaagcaga agattatttc ttcaataagt
     1381 ttctgttgtc ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt
     1441 acggaacggc tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg
     1501 aagccaaacc aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt
     1561 taaaatggaa taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg
     1621 ggataagcaa tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa
     1681 attaccagaa cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata
     1741 ttcatctttg gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct
     1801 acggagtatc attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg
     1861 gcaaagcggt gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa
     1921 actaacgctt cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc
     1981 gaaaattata tgaacgcaat aatatcgccc gattattact atgttcttac cgttgctggt
     2041 cagtctaatg ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct
     2101 cattccagaa ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgccatgt
     2161 cactttaacg acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt
     2221 tatcaccatc ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg
     2281 catattgcac ggaaattact gcccttcatt cctaataatg cagggattct tatcgttccg
     2341 tgttgccgtg gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac
     2401 ggagccagcc atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc
     2461 agcagaacgc gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg
     2521 atgcaaggcg aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat
     2581 catatggttg aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt
     2641 actgacgcgc catggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat
     2701 tcgtatgaag ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc
     2761 gacttccagc aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat
     2821 ttaagcacgg gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg
     2881 cgaagcagtc atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa
     2941 gcaattttgc agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt
     3001 gttcgttctc agagtagaca acaaaaaaca aataagatca aggtaatatc tcttcacttg
     3061 cgggctcgaa cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc
     3121 accgcataaa ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc
     3181 cttttcgatt atggctcgga aggtaatgac tc
//
LOCUS       DAZRUF010000320         3190 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZRUF010000320 DAZRUF010000000
VERSION     DAZRUF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.323"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            87..1145
                     /gene="rsxD"
                     /locus_tag="ACHXOL_004030"
     CDS             87..1145
                     /gene="rsxD"
                     /locus_tag="ACHXOL_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR6810865.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            1149..1769
                     /gene="rsxG"
                     /locus_tag="ACHXOL_004031"
     CDS             1149..1769
                     /gene="rsxG"
                     /locus_tag="ACHXOL_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR6810866.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            1773..2468
                     /gene="rsxE"
                     /locus_tag="ACHXOL_004032"
     CDS             1773..2468
                     /gene="rsxE"
                     /locus_tag="ACHXOL_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR6810867.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            2468..3103
                     /gene="nth"
                     /locus_tag="ACHXOL_004033"
     CDS             2468..3103
                     /gene="nth"
                     /locus_tag="ACHXOL_004033"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR6810868.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT          739 a          810 c          843 g          798 t
ORIGIN      
        1 atccgcgcaa agcggcagtt gccgcggcta ttgcccgcgc tcaggccaaa aaagccgccc
       61 agcagaaggt tgtaaacgag gactaaatgg tattcagaat agctagctcc ccttataccc
      121 ataaccagcg ccagacatcg cgcattatgc tgttggtgtt gctcgcagcc gtgccaggaa
      181 tcgcagcgca actgtggttt tttggttggg gtactctcgt tcagatcctg ttggcatcgg
      241 ttagtactct gttagccgaa gctctcgtac tcaaactacg caagcagtcg gtagccgcaa
      301 cgttgaaaga taactcagca ttgctgacag gcttattgct ggcggtaagt attccccccc
      361 tcgcgccatg gtggatggtc gtgctgggta cggtgtttgc ggtgatcatc gctaaacagt
      421 tgtatggcgg tctggggcaa aacccgttta atccggcaat gattggttat gtggtcttac
      481 tgatctcctt cccggtgcag atgaccagct ggttaccgcc acatgaaatt gcggtcaaca
      541 tccctggttt tatcgacgcc atacaggtta ttttcagcgg gcataccgcc agtggtggtg
      601 atatgaacac actacgctta ggtattgatg gcattagtca ggcgacaccg ctggatacat
      661 ttaaaacctc tgtccgtgcc ggtcattcgg ttgaacagat tatgcaatat ccgatctaca
      721 gcggtattct ggcgggcgct ggttggcaat gggtaaatct cgcctggctg gctggcggcc
      781 tgtggttgct atggcagaaa gcgattcgct ggcatattcc cctcagcttc ttagtaacgc
      841 tggcgttatg cgcaacgttg ggctggttgt tctcaccaga aacactggca gcaccgcaaa
      901 ttcatctgct gtctggtgcg accatgctcg gcgcattctt tattttgact gacccggtta
      961 ccgcttctac gaccaatcgt ggtcgtctta ttttcggcgc gcttgcgggc ttattagtct
     1021 ggttgatccg cagtttcggc ggctatcctg acggcgtggc ttttgccgtc ctgctggcga
     1081 acatcacggt tcctctgatc gattactaca cgcgtccgcg cgtctacggc catcgcaaag
     1141 ggtaaaccat gctgaaaact atccgaaaac acggcattac gttagcgcta tttgcagcgg
     1201 gttcaacagg gttaactgcg gccatcaacc agatgaccaa aacgacgatt gctgaacagg
     1261 ccagtctgca acaaaaggca ttatttgatc aggtgctgcc agccgaacgc tataacaata
     1321 cgctggcaca gagttgctat ctggtaactg cgccagagtt aggtaaaggt gagcatcggg
     1381 tttacatcgc caaacaggat gacaaaccgg tagccgccgt tctggaagca accgcgccag
     1441 atggctattc cggtgcgatt cagctgctgg tgggagccga ttttaacggc acggtacttg
     1501 gcacgcgcgt gacagagcac cacgaaacgc cagggcttgg cgataaaatc gaactgcgcc
     1561 tttctgactg gatcacccat tttgcgggta aaaaaatcag tggtgcggat gatgcgcact
     1621 gggcggtgaa gaaagatggt ggtgatttcg accagttcac cggcgcgacg attactcccc
     1681 gcgcggtggt taatgcggta aaacgcgccg gattgtacgc tcagacgtta ccggcacaac
     1741 tttctcaact tcctgcctgt ggagaataaa gcgtgagcga aattaaagac gttattgttc
     1801 aggggttgtg gaaaaacaac tctgcgctgg tccagttgct cggcctttgt cctctgttgg
     1861 cggtcacgtc cactgccact aacgctctgg gtttaggact tgcgactacg ctggtactga
     1921 cgctgaccaa cctgaccatt tcgacgctgc gtcactggac gccagccgag atccgcattc
     1981 ccatttacgt gatgatcatc gcctcggtgg tcagcgctgt acagatgctg atcaacgcct
     2041 acgcctttgg cctgtatcaa tcattaggga tttttattcc gctgattgtc actaactgta
     2101 tcgttgtggg ccgcgctgaa gccttcgccg ccaaaaaagg tccggcgctt tcggcactgg
     2161 acggcttttc aattggtatg ggcgcaacct gcgccatgtt cgtgctgggt tcactacgcg
     2221 aaattatcgg caatggcaca ttgtttgacg gtgcagatgc gctgttaggt agctgggcaa
     2281 aagtattacg cgtggagatt ttccacaccg actccccttt cctgctggcg atgctgccac
     2341 caggtgcatt tattggcctg ggactgatgc tggcaggaaa atacctgatt gatgaaagaa
     2401 tgaaaaagcg ccgtactgaa gcagctgccg aacgtgcatt gccaaacggt gaaacaggga
     2461 atgtctgatg aacaaagcaa aacgcctgga gatcctcact cgcctgcgtg agaacaatcc
     2521 tcatcccacc accgagctta atttcagttc gccttttgaa ttgctgattg ccgtactgct
     2581 ttccgctcag gcgaccgatg tcagtgttaa taaggcgacg gcgaaacttt acccggtggc
     2641 gaatacgcct gcagcgatgc ttgaactggg cgttgaaggg gttaaaacct atatcaaaac
     2701 gattgggctt tataacagca aagcagaaaa tatcatcaaa acctgccgta tcttactgga
     2761 gcagcataat ggcgaggttc cggaagatcg tgctgcgctt gaagccctgc ccggcgtagg
     2821 tcgtaaaaca gccaacgtcg tattaaacac tgcattcggc tggccgacta ttgctgtcga
     2881 cacgcacatt ttccgcgttt gtaatcgtac tcaatttgcg ccggggaaaa acgtcgaaca
     2941 ggtagaagaa aagctactga aagtggttcc ggcagagttt aaagtcgact gccaccattg
     3001 gttgatcctg cacgggcgtt atacctgcat tgcccgcaag ccccgctgtg gctcttgtat
     3061 tattgaagat ctttgtgaat acaaagagaa agttgacatc tgaagaaaag gggtaacacc
     3121 gattacccca ttgataacct ttctttatcc tcttttaaaa catcttttta acgtgaatga
     3181 tgccattgct
//
LOCUS       DAZRUF010000321         3172 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZRUF010000321 DAZRUF010000000
VERSION     DAZRUF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.324"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHXOL_004034"
                     /gene_synonym="dgcC"
     CDS             complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHXOL_004034"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR6810869.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHXOL_004035"
     CDS             complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHXOL_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR6810870.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHXOL_004036"
     CDS             complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHXOL_004036"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR6810871.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHXOL_004037"
     CDS             complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHXOL_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR6810872.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          737 a          856 c          762 g          817 t
ORIGIN      
        1 ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca
       61 tgaccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca
      121 tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt
      181 cggttataca cgcccgtcat gccgtcgcga gtactcatga cctgcaacct gcgtttatgt
      241 tccgccagtt tggttgccgt ctggtagctg acccagccaa acagcagagg ataaacgaca
      301 ataatgggaa gggagagcca ccattccagc ggcgcactat tgaacgacac ggtaatgccc
      361 gtaagctcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga
      421 cgggggccgc ctgcccccat caaattcaga cacataatca tcaacatagc ggtggaaggc
      481 agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag
      541 ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc
      601 ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa
      661 accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg gggtagtctg
      721 acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt
      781 tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac
      841 attcagaaac aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc
      901 gggtgagcca ctttcgtagc tcacccgtta gcactatcag gtatcaggcg gctttcttga
      961 ggcaggcact cataaactta ttacgatcat cacctttcag agattgttgc gttgcttgat
     1021 tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagact
     1081 tgctgttctt caggcaatca ctcatgtagg tcttacgagc atcccctttc aacgcctgcg
     1141 ccgtcgcctg ctgattacag gaggtcatac gctgttgttg tggggttaag gttctctcgg
     1201 cagcgccgac ggtggttaaa aaaaccagac caaaaagcaa ggtaaccagt aatgttattt
     1261 tcatagcacc atccctcttc atgttttaac catgagcgtt tgcgcccgtg atctgccatt
     1321 aagtctggtt gctaacagca aaaaaaccac ccggcagcga aaattcactg ccgggcgcgg
     1381 ttttatttca gccccagagc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt
     1441 ccaacaacat tggcggcatg cggaccatac gccgcaatac gcaactgact gccggtatgt
     1501 tcttgtgaat cctcttcgga attcccgtaa ctcatcacca tcactgcgcc atctttagta
     1561 tttagcgcct gagtgaggcc cggcgctttg gtatccggcg caacaacctg gctggcgtgg
     1621 gcgtgatcag cggtgactat gaccagcgtg ttaccctcct ttttagcgaa ttccagcgcc
     1681 cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg
     1741 tgatcctgtt tatcgattga cgcaccttca acttgcagga aaaatccttt ctcattttta
     1801 ctcaacaatt caatggtttt gtcggtcatc tgtgccaggg ttggtacact gtcattacgt
     1861 tgcggattag gcgtacaggt gactgcgggc ttatcgatat tgccgtggta cgttgctttc
     1921 ggccctagcc agcgcactgg catattgccg tcagcaaaca ggccaagcag gggtttttgc
     1981 tgattcgctt ccgtcaccga attcagtgag gcagcatcgt tcaccaactg ataaccacgc
     2041 gcctgtgcct gttcacgcag cgtttttccc tgccattcac cagcggttgc cgtttcagca
     2101 aaggtttttg cgccgccgcc aagcgtaacg tcggcacgag cgttaagcag ctgttcggta
     2161 atcgatcctt ttccgccttt ttccagagcg ttacccggac atttttcact ggtcgcgctc
     2221 ggaccgtagc atttgcgcga agtcacatgt gccaccagcg cagcgggcgt ggcatcctgc
     2281 aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc
     2341 gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cgttataggt tttgacaccg
     2401 gttgaccagg cggttgctga tgcagccgag tcggtgacgt agtccggttt gccagttttt
     2461 ttattcagcg catagtgagt gtattgcccg gtaagcggta aggcatcgat acctttaaaa
     2521 aagccgcccg caccttcggc ataattacgt gcggcagtaa tttccgaatc ccccatccca
     2581 tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc acgcagagcg
     2641 gcggtctgat cactcgttaa gcggcgagca ccgccgggtg cagtaatatc gccctgagca
     2701 gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg ggtaaacagt
     2761 aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat
     2821 acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt tatgacagct
     2881 gttgaaaaga ttaacttttt actgacgagg atgcttcaat aacttcttta cgtaatcgcg
     2941 cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc
     3001 ctctacctga tcaatcaacc gctgttggtc attttgcgcc atattgcgaa gcattgcagt
     3061 gacgataatc tccaaagctt ctacctgcgc acacagttct ttcgactctt cttctttttg
     3121 ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc ct
//
LOCUS       DAZRUF010000322         3095 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZRUF010000322 DAZRUF010000000
VERSION     DAZRUF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.325"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            73..1755
                     /locus_tag="ACHXOL_004038"
     CDS             73..1755
                     /locus_tag="ACHXOL_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR6810873.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1807..2964
                     /locus_tag="ACHXOL_004039"
     CDS             1807..2964
                     /locus_tag="ACHXOL_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR6810874.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2930..3052)
                     /locus_tag="ACHXOL_004040"
     CDS             complement(2930..3052)
                     /locus_tag="ACHXOL_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810875.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESIINYSPFVLQHP"
BASE COUNT          942 a          666 c          621 g          866 t
ORIGIN      
        1 cttcacttac atccacttat ttctcttcgt aaaattactt cggaattaag tacaataaga
       61 agaggaacat ttatgaagtc tgcattaaag aaaagtgtcg taagtacctc gatatctttg
      121 atactggcat ctggtatggc tgcatttgct gctcatgcgg cagatgatgt aaagctgaaa
      181 gcaaccaaaa caaacgttgc tttctcagat tttacgccga ctgaatacag taccaaagga
      241 aagccaaata ttatcgtact aaccatggat gatcttggtt atggacaact tccttttgat
      301 aagggatctt ttgacccaaa aacaatggaa aatcgtgaag ttgtcgatac ctacaaaata
      361 gggatagata aagccattga agctgcacaa aaatcaacgc cgacgctcct ttcattaatg
      421 gatgaaggcg tacgttttac taacggctat gtggcacacg gtgtttccgg cccctcccgt
      481 gccgcaataa tgaccggtcg agctcccgcc cgctttggtg tctattccaa taccgatgct
      541 caggatggta ttccgctaac agaaactttc ttgcctgaat tattccagaa tcatggttat
      601 tacactgcag cagtaggtaa atggcacttg tcaaaaatca gtaatgtgcc ggtaccggaa
      661 gataaacaaa cgcgtgacta tcatgacaac ttcaccacat tttctgcgga agaatggcaa
      721 cctcaaaacc gtggctttga ttactttatg ggattccacg ctgcaggaac ggcatattac
      781 aactcccctt cactgttcaa aaatcgtgaa cgtgtccccg caaaaggtta tatcagcgat
      841 cagttaaccg atgaggcaat tggcgttgtt gatcgtgcca aaacacttga ccagcctttt
      901 atgctttacc tggcttataa tgctccgcac ctaccaaatg ataatcctgc accggatcaa
      961 tatcagaagc aatttaatac cggtagtcaa acagcagata actactacgc ttccgtttat
     1021 tctgttgatc agggtgtaaa acgcattctc gaacaactga agaaaaacgg acagtatgac
     1081 aatacaatta ttctctttac ctccgataat ggtgcggtta tcgatggtcc tctgccgctg
     1141 aacggggcgc aaaaaggcta taagagtcag acctatcctg gcggtactca caccccaatg
     1201 tttatgtggt ggaaaggaaa acttcaaccc ggtaattatg acaagctgat ttccgcaatg
     1261 gatttctacc caacagctct tgatgcagcc gatatcagca ttccaaaaga ccttaagctg
     1321 gatggtgttt ccttgctgcc ctggttgcaa gataagaaac aaggcgcgcc acataaaaat
     1381 ctgacctgga taacctctta ttctcactgg tttgatgagg aaaatattcc attctgggat
     1441 aattaccaca aatttgttcg ccatcagtca gacgattacc cgcataaccc caacactgag
     1501 gacttaagcc aattctctta tacggtgaga aataacgatt attcgcttgt ctatacagta
     1561 gaaaacaatc agttaggtct ctacaaactg acggatctac agcaaaaaga taaccttgcc
     1621 gccgccaatc cgcaggtcgt taaagagatg caaggcgtgg taagagagtt tatcgacagc
     1681 agccagccac cgcttagcga ggtaaatcag gagaagttta acaatatcaa gaaagcacta
     1741 agcgaagcga aataactaaa ccttcatgcg gcggattttt ccgccgcctt attgagcgag
     1801 atagcgatgc acgttacagc caagccctcc agttttcaat gtaatctcaa atgtgattac
     1861 tgtttttacc ttgaaaaaga gtcgcagttt actcatgaaa aatggatgga tgacagcact
     1921 ctgaaagagt tcatcaaaca atatatcgaa gcgtctggca atcaggtcta ttttacctgg
     1981 caaggcggtg aacccactct ggctggcctg gattttttcc gtaaagttat tcactatcaa
     2041 caacgctatg caggccaaaa acgtattttt aatgcattac aaacgaatgg cattttattg
     2101 aataatgaat ggtgtgcctt tctcaaagaa catgaatttc tggttggtat ctcgatcgat
     2161 ggcccccagg agttacatga ccgttacaga cgcagtaatt caggtaacgg tacttttgca
     2221 aaagtgatag cagccatcga gcgtctgaaa tcatatcaag tagagtttaa tacgttaacc
     2281 gtcattaata acgttaatgt ccattaccct cttgaggttt atcatttttt aaaatctatc
     2341 ggcagtaaac atatgcaatt tatcgaattg ctagaaaccg ggacgccgaa tattgatttc
     2401 agtggtcata gtgagaacac attccgtatc attgattttt ctgtgcctcc cacggcttat
     2461 ggcaagttta tgtcaaccat ttttatgcaa tgggttaaaa acgatgtggg tgaaattttc
     2521 attcgtcagt ttgaaagctt tgtcagccgt tttttgggga atgggcatac aagttgtatt
     2581 ttccaggagt cctgcaagga taatctggtt gttgaaagta atggagacat ttacgaatgc
     2641 gaccattttg tctatccaca gtacaaaatt ggaaacatta ataaatctga actcaaaacg
     2701 atgaacagtg tacaactgac agcgcaaaaa aaacggattt cagcgaaatg tcagcaatgt
     2761 gcatataaac ctatctgcaa tggcggttgt cctaagcatc gtattactaa agtaaacaat
     2821 gagactgttt cttatttttg cgaaggttat aaaatccttt tttcaaccat ggtaccttat
     2881 atgaacgcca tggttgagtt agctaagaac agagtaccgc tttaccacat tatggatgtt
     2941 gcaaaacaaa tggagaataa ttaataatgc tctcttttaa ctccattctt agcctccttc
     3001 ttatgacctt aacaaggcac ccgacataaa ttcctgtcgg gtgtttcgtc atcatcatct
     3061 tcccttgcga aaatcctggc ttacgtggta tgcgt
//
LOCUS       DAZRUF010000323         3045 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZRUF010000323 DAZRUF010000000
VERSION     DAZRUF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.326"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..172
                     /locus_tag="ACHXOL_004041"
     CDS             <1..172
                     /locus_tag="ACHXOL_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810876.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="ACHXOL_004042"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="ACHXOL_004042"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFR6810877.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..2133
                     /gene="allB"
                     /locus_tag="ACHXOL_004043"
                     /pseudo
     CDS             955..2133
                     /gene="allB"
                     /locus_tag="ACHXOL_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2190..>3045
                     /locus_tag="ACHXOL_004044"
     CDS             2190..>3045
                     /locus_tag="ACHXOL_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR6810878.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          695 a          710 c          836 g          804 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     1201 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     1261 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     1321 ttggtgcact gcgaaaacgc gctgatctgt gacgcactgg gcgaagaagc gaaacgtgaa
     1381 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     1441 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     1501 agcagcccgg aaagtgttga agaagtgact cgtgcacgtc aggaaggtca ggatgttacc
     1561 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggtact
     1621 ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc agaaaggcat gtgggaaaaa
     1681 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     1741 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     1801 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     1861 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     1921 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     1981 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     2041 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     2101 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     2161 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     2221 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     2281 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     2341 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     2401 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggcgaagcat
     2461 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     2521 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     2581 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc acagctgacc actatctttt
     2641 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc atattttaaa attcagttac
     2701 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     2761 cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat aaccggctgg ttgttgtggt
     2821 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     2881 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     2941 taaatatcag caatacctac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     3001 gcgcggggaa tacgcgttat cgtcgcagct ttgtggcgac cggat
//
LOCUS       DAZRUF010000324         2992 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZRUF010000324 DAZRUF010000000
VERSION     DAZRUF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.327"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHXOL_004045"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHXOL_004045"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR6810879.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHXOL_004046"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHXOL_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR6810880.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHXOL_004047"
     CDS             2805..>2992
                     /locus_tag="ACHXOL_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR6810881.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZRUF010000325         2958 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZRUF010000325 DAZRUF010000000
VERSION     DAZRUF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.328"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            76..789
                     /locus_tag="ACHXOL_004048"
     CDS             76..789
                     /locus_tag="ACHXOL_004048"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR6810882.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            821..1579
                     /locus_tag="ACHXOL_004049"
     CDS             821..1579
                     /locus_tag="ACHXOL_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810883.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1625..>2958
                     /locus_tag="ACHXOL_004050"
     CDS             1625..>2958
                     /locus_tag="ACHXOL_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR6810884.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          658 a          727 c          803 g          770 t
ORIGIN      
        1 tcacaataag gttgaacgga tattctgagc acggaccacg taatattcaa tacattattt
       61 actgccgggt ttttcgtgaa tcaacgtaat atgtcaataa ttaattccac gccagtgcgt
      121 gttattgcca ttgtaggatg tgatggttca ggtaaatcga ccctcacggc aagcctggta
      181 aatgaactgg cagcaagaat gccaacagaa cacatttatc tcgggcaatc gtccgggcta
      241 attggcgaat ggatttcaca gctccctgtt attggcgcac cttttgggcg ttatctgcga
      301 agtaaagcgg cacatgtgca cgaaaaaccc tcaacaccgc ctggcaatat tactgctctg
      361 gttatctatc tgctttcctg ctggcgggcg tacaagtttc gcaaaatgtt gtgtaaaagc
      421 cagcaaggct ttctgctcat caccgaccgc tacccgcagg ttgaagtgcc gggttttcgc
      481 tttgatggcc cgcaattggc aaaaaccacg ggcggtaacg gttggataaa aatgttaagg
      541 cagcgcgagc tgaagctgta ccaatggatg gcatcttatt tgcccgtatt gttgattcgt
      601 cttggcattg atgaacaaac cgcgtttgcg cgtaaacctg accaccaact ggcagcgtta
      661 caggaaaaaa tcgccgttac gccgcaactg actttcaacg gcgccaggat ccttgaactg
      721 gatggtcggc aaccagccga tgaaatcctg caagcgtcac tacgtgcaat tcacgccgcg
      781 ctttcctgat caccaaaatg gataggggat gatttaacga atggatgcac tacaaacaca
      841 aactgttaat agtacaaccg cgccgcagcc cagctacatt ccggggctaa ttgcggtggt
      901 cgggtgcgat ggcaccggta aatccacgct gaccaccgat ctggtgaaat cattgcaaca
      961 gcactggcaa accgagcggc gctatctggg gctgctctcg ggcgaagacg gcgacaaaat
     1021 caaacgatta ccgttggttg gcgtctggct ggaacggcga ctggcggcca aatcctcgaa
     1081 aacccaaagc atgaaaacca aatctccggc gctatgggcg gcggtgatta tgtactgctt
     1141 ctcgctgcga agaatggcga atctacgcaa ggttcagcga ctggcgcaaa gtggcgttct
     1201 ggtggtcagc gatcgcttcc cgcaggctga aatttcgggc ttttattacg acggaccggg
     1261 gattggcgtc gaacgtgcga ccgggaaaat cagcatgttt ctggcgcagc gcgaacggcg
     1321 tttataccaa caaatggcgc aatatcgccc ggaattaatt attcgcctgg gcattgatat
     1381 tgagactgcc atctcccgca agcctgacca tgactatgcc gagctgcagg acaaaatcgg
     1441 tgtcatgtcg aagattggct ataacggcac aaaaattctt gagattgatt ccagagcgcc
     1501 ctacagcgaa gtgctggaac aggcacagaa ggcggtttct ctggttgcca tcgtttctga
     1561 ccgccgaagt ttaacttaag gctggcggga tactttttac tatcaacgat aaggtcaggc
     1621 tgaattggcg ggttttaaca tcaaacattg gtttgcagat ggcgcgtttc gcaccattat
     1681 tcgcaatagc gcctggttag gctccagtaa tgtcgtgagc gccttgctgg ggctgttggc
     1741 gctctcgtgt gccggtaaag ggatgacgcc cgccatgttt ggcgtactgg tgattgtgca
     1801 atcgtacgcc aagtcgatca gcgattttat taagtttcag acatggcagc tggtggttca
     1861 gtacggaacg ccagccttga ccaacaataa tccgcagcaa tttcgcaatg tcgtctcatt
     1921 ttccttctcg ctggatatcg tcagcggcgc ggtggcgatt gtcggcggca ttgccttact
     1981 gccattcctt tcccattcat taggtctgga tgaccaaagc ttttggctgg cggcgctgta
     2041 ttgcacgctc attccttcaa tggcttcctc cacgccgacc ggcattctgc gtgcggtaga
     2101 tcgcttcgat ttaattgctg tacagcaggc gacgaaacct tttctgcgcg cagcggggag
     2161 cgtcgtagcc tggtattttg actttggttt tgcgggtttt gttattgcct ggtacgtgtc
     2221 gaatctggtt ggcggcacca tgtactggtg gtttgcagcg cgcgaattac gccgcagaaa
     2281 tatccataat gccttcaaat tgaatctgtt tgagtctgcc cgacacatca aaggcgcgtg
     2341 gagttttgtc tggtcaacca acattgccca ctccatctgg tcggcgcgta actcgtgcag
     2401 cacagtgctg gtggggatcg tgttaggacc cgctgccgcc gggttattta aaatcgccat
     2461 gacattcttc gacgccgccg gaacgccagc gggtttgctg ggtaaaagtt tttacccaga
     2521 ggtgatgcgt ttagatccgc gcaccaccag accgtggttg ttaggtgtga agtccggttt
     2581 actggcgggc ggaatcggta ttctggtggc gctcgcggtg ttgatcgccg gcaagccgct
     2641 catttcgctg gtgtttggcg ttaagtatct cgaagcgtat gacctgattc aggtgatgct
     2701 gggcgcaatt gtgatttcga tgctgggttt cccgcaggag tcattgctat tgatggcggg
     2761 aaaacagcgc gcttttctcg tggcgcaaac catcgcctca atcggctaca tcgtattgct
     2821 gttcatgttc tgtcatctgt ttggtgtgct gggagcagcg tttgcttact tcggtgggca
     2881 atgtctggat gtggtgctct cgttaattcc aactttaaag gcattttttc agcgtcattc
     2941 cttgctttat aacgcagc
//
LOCUS       DAZRUF010000326         2951 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZRUF010000326 DAZRUF010000000
VERSION     DAZRUF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.329"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(182..1668)
                     /locus_tag="ACHXOL_004051"
                     /pseudo
     CDS             complement(182..1668)
                     /locus_tag="ACHXOL_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
     gene            complement(1695..>2951)
                     /locus_tag="ACHXOL_004052"
     CDS             complement(1695..>2951)
                     /locus_tag="ACHXOL_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR6810885.1"
                     /translation="RWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSNTEIGLIM
                     STFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVMLCIQVAFA
                     ITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFSRFAPDDS
                     ASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVLRISTTWY
                     CSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPLGGIITTY
                     SKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYASRGLYWAC
                     PGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWLMGMAALG
                     MVIVFTFLLFQKIRTADSAPAMASSK"
BASE COUNT          777 a          778 c          669 g          727 t
ORIGIN      
        1 ttccttgtaa aaacagggat aaaccgaact agttaaagtt tttattatgt tccctgcatc
       61 agcggggata aaccggactt gttatcattt tttaagttgt cgaacttatg ttccccgcgc
      121 cagcggggat aaccattcag gtatgttcag gccgggcagt tttccgcccg gcctacctta
      181 ttcacgcatt cgaccagctc tggatcgaat ccgcattccc caattcccca attccccatg
      241 catcacttca tacgattttt tcaggatcac atcagtatgt ttggttaggt ctttgaaaat
      301 gcctttattc atcgcgtcgt agcgtttggc attgctctca atcggtataa agatatcttt
      361 cacgcgaacc attttatcga cagccgttgc gtaatccggg tatagcccca gacctacccc
      421 tgtattaatc gctgccccca ggcttgcaca accgttaatg gcgttacggc gtgccggaag
      481 gttgaacacg tcggcaaaaa tctgcataaa cagatcgctg ttcgaaccgc cgccagtaat
      541 gattacatgt ttcgcaaagt gattcatttc attacacata ttgtcgtaat tgttcttcag
      601 cgtcagcgcc acgctttcca atatcgaacg atatatccat gcgtaatcca tgctggaatc
      661 aaagccgatc ataatcccgc gtttgtacgg ttcccacgga ttggtcagcc agtccagcac
      721 cgtcatcagc ccattacagc caggtggcac actagaggct ttcttgttga gtaaatcttc
      781 cggtgaaaga tcctgcgccc tggcatcctg aattaacgac tcgcctaaca tgtcgcgcag
      841 ccagctcacc gtccacatgc ctttgcgaat accgtaacct tcatacagca atgtttgcgg
      901 aatagaagac ataatcggcc agtacgccac cggatctttc ggcaaggctt tgccgttcat
      961 catcaatgcg atgtaagtgc caagagaaat taccgcagtt tcatcatcca gtaatccggc
     1021 tcccagagct tctaccggtt tatcactggt ggtacaaaca accggcagtc ctgccgggaa
     1081 atgcgtcgcc agcgcggctt gaggtgtgat atgcccaaga acagtgcctg gcatttgcac
     1141 atcaaacagc atatgacggg ggatattaaa cttctccatg actgcggcat cttcgctcca
     1201 tgcccaggtc ttataatcca ccggccactg accaaaatag ttggcgatat tatctttaaa
     1261 ttcgccggtt aagcgatgcg tcagataacc cgaaaaagag gttacatacg ccacgtcagg
     1321 attcgtatgt tcgtaagggc gtgtaacgcg tgcatcctgc cagctaatca acggcgcagc
     1381 tggcgtgccg tcggctttca ataacgcacg gcagcaacgg atggagccca ggccgatgcc
     1441 aacaatatct tctttattcc cggcaaactg gctcatcaaa tcgtgacctg caaaacataa
     1501 tgatgcccat aaatcgtcgt caggatgttc tgcagtatcg gcatcaggcg tgtgcatcgg
     1561 ctgtaataag cctttacctt cgcaaaccac attaccttcc agatcgtaca tcaccacttt
     1621 tgtgctctga cttccgccat caatccctat gatgtatttt ttcgacatga tttttctcct
     1681 gtaattttcc tggcttactt gctgctagcc attgcggggg cgctatcagc agtacgaatt
     1741 ttttggaaca gcaaaaaggt aaagacaatc accatgccga gcgccgccat gcccatcagc
     1801 cacatattgc ggtaggcttc tgcagcgggc agggtgtctt gccagtggcc gataatcggg
     1861 taaacgaaga catccggcag gaatccaatc accgaacaaa tacctaccgt ggtgcccata
     1921 atgtaagacg gtgttctcgc ttcgccgggg caggcccagt acagcccgcg tgaggcgtaa
     1981 caggtgaatc ccagcagtaa aatcaggcca atccccatcg cgaccgattg ggggttagag
     2041 ttcgtgacga gcagggctgt caacgccagc aaaccgatga tggaaaggat ttgaatcacg
     2101 cgggtagggg atttcacttt actgtaggtg gtgataatgc ctccaagtgg gccgcacagc
     2161 gcgcggaaga ttttgttgat cacaatcccc atgtagctcg ctgccaccag cgacatgccg
     2221 tacatttcgg tcagatagtt ggtggagtaa ctcagaatgg cgtagattgt gaagacacca
     2281 aaaatcacca tgctgcaata ccaggtggtg ctgatacgca aaacggcaag gatgtcgcta
     2341 agctggaatg actgtttttc ttcgttattg gcactgcgca ggttgttgtt atcgctaaca
     2401 aaaaaccagc acagaatccc caacaagatg taaaccacac tgtagatgat aatgactgtt
     2461 ttcaggctgg cgctgtcatc cggtgcaaag cgagaaaaga cccacatggt aaacaccgcc
     2521 agcgacatta caccgacgcc gcgcagccct tccatccagc ccataatttt cccttgctca
     2581 ctatgatcgc ccagcaacga ggcggcttta atcgacaccg accacagcat taaaatcgtc
     2641 gttatcgcaa aggcgacctg aatacagagc attacccaca gcggtggata cgttgccatt
     2701 aacagaccca gtaatccggt aatgatcatc gcggaagtaa tcattttgcg gtgtgaaaat
     2761 ttatcggcaa taacgccgct gggggcataa agaataatag ccgcaatacc aaaggtactc
     2821 attattaaac ctatctcggt attgctgaat cccataaatt tcgccatggg aatttgataa
     2881 atataacgta aataagccag gtcaaaacta acgccgccgc taaaactaat tatcgcgagg
     2941 gttatccaac g
//
LOCUS       DAZRUF010000327         2913 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZRUF010000327 DAZRUF010000000
VERSION     DAZRUF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.330"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            200..2875
                     /gene="adhE"
                     /locus_tag="ACHXOL_004053"
                     /gene_synonym="adhC"
     CDS             200..2875
                     /gene="adhE"
                     /locus_tag="ACHXOL_004053"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR6810886.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPAARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT          756 a          740 c          707 g          710 t
ORIGIN      
        1 atcttgctta cgccacctag aagtgacgca ttagagataa taactctaat gtttaaactc
       61 ttttagtaaa tcacagtgag tgtgagcgcg agtaagcttt tgattttcat aggttaagca
      121 aatcatcacc gcactgacta tactctcgta ttcgagcaga tgatttacta aaaaagttta
      181 acattatcag gagagcatta tggctgttac taatgtcgct gaacttaacg cactcgtaga
      241 gcgtgtaaaa aaagcccagc gtgaatatgc cagtttcact caagagcaag tagacaaaat
      301 cttccgcgcc gccgctctgg ctgctgcaga tgctcgaatc ccactcgcga aaatggccgt
      361 tgccgaatcc ggcatgggta tcgtcgaaga taaagtgatc aaaaaccact ttgcttctga
      421 atatatctac aacgcctata aagatgaaaa aacctgtggt gttctgtctg aagacgacac
      481 ttttggtacc atcactatcg ctgaaccaat cggtattatt tgcggtatcg ttccgaccac
      541 taacccgact tcaactgcta tcttcaaatc gctgatcagt ctgaagaccc gtaacgccat
      601 tatcttctcc ccgcacccgc gtgcaaaaga tgccaccaac aaagcggctg atatcgttct
      661 gcaggctgct atcgctgccg gtgctccgaa agatctgatc ggctggatcg atcaaccttc
      721 tgttgaactg tctaacgcac tgatgcacca cccagacatc aacctgatcc tcgcgactgg
      781 tggtccgggc atggttaaag ccgcatacag ctccggtaaa ccagctatcg gtgtaggcgc
      841 gggcaacact ccagttgtta tcgatgaaac tgctgatatc aaacgtgcag ttgcatctgt
      901 actgatgtcc aaaaccttcg acaacggcgt aatctgtgct tctgaacagt ctgttgttgt
      961 tgttgactct gtttatgacg ctgtacgtga acgttttgca acccacggcg gctatctgtt
     1021 gcagggtaaa gagctgaaag ctgttcagga tgttatcctg aaaaacggtg cgctgaacgc
     1081 ggctatcgtt ggtcagccag cctataaaat tgctgaactg gcaggcttct ctgtaccaga
     1141 aaacaccaag attctgatcg gtgaagtgac cgttgttgat gaaagcgaac cgttcgcaca
     1201 tgaaaaactg tccccgactc tggcaatgta ccgcgctaaa gatttcgaag acgcggtaga
     1261 aaaagcagag aaactggttg ctatgggcgg tatcggtcat acctcttgcc tgtacactga
     1321 ccaggataac caaccggctc gcgtttctta cttcggtcag aaaatgaaaa cggctcgtat
     1381 cctgattaac accccagcgt ctcagggtgg tatcggtgac ctgtataact tcaaactcgc
     1441 accttccctg actctgggtt gtggttcctg ggggggtaac tccatctctg aaaacgttgg
     1501 tccgaaacac ctgatcaaca agaaaaccgt tgctaagcga gctgaaaaca tgttgtggca
     1561 caaacttccg aaatctatct acttccgccg tggctccctg ccaatcgcgc tggatgaagt
     1621 gattactgat ggccacaaac gtgcgctcat cgtgactgac cgcttcctgt tcaacaatgg
     1681 ttatgctgat cagatcactt ccgtactgaa agcagcaggc gttgaaactg aagtcttctt
     1741 cgaagtagaa gcggacccga ccctgagcat cgttcgtaaa ggtgcagaac tggcaaactc
     1801 cttcaaacca gacgtgatta tcgcgctggg tggtggttcc ccgatggacg ccgcgaagat
     1861 tatgtgggtt atgtacgaac atccggaaac tcacttcgaa gagctggcgc tgcgctttat
     1921 ggatatccgt aaacgtatct acaagttccc gaaaatgggc gtgaaagcga aaatgatcgc
     1981 tgtcaccacc acttctggta caggttctga agtcactccg tttgcggttg taactgacga
     2041 cgctactggt cagaaatatc cgctggcaga ctatgcgctg actccggata tggcgattgt
     2101 cgacgccaac ctggttatgg acatgccgaa gtccctgtgt gctttcggtg gtctggacgc
     2161 agtaactcac gccatggaag cttatgtttc tgtactggca tctgagttct ctgatggtca
     2221 ggctctgcag gcactgaaac tgctgaaaga atatctgcca gcgtcctacc acgaagggtc
     2281 taaaaatccg gcagcgcgtg aacgtgttca cagtgcagca actatcgcgg gtatcgcatt
     2341 tgcgaacgcc ttcctgggtg tatgtcactc aatggcgcac aaactgggtt cccagttcca
     2401 tattccgcac ggtctggcaa acgccctgct gatttgtaac gttattcgct acaatgcgaa
     2461 cgacaacccg accaagcaga ctgcattcag ccagtatgac cgtccgcagg ctcgccgtcg
     2521 ttatgctgaa attgccgacc acttgggtct gagcgcaccg ggcgaccgta ctgctgctaa
     2581 gatcgagaaa ctgctggcat ggctggaaac gctgaaagct gaactgggta ttccgaaatc
     2641 tatccgtgaa gctggcgttc aggaagcaga cttcctggcg aacgtggata aactgtctga
     2701 agatgcattc gatgaccagt gcaccggcgc taacccgcgt tacccgctga tctccgagct
     2761 gaaacagatc ctgctggata cctactacgg tcgtgattat gtagaaggtg aaactgcagc
     2821 gaaaaaagaa gccgctccgg ctaaagctga gaaaaaagcg aaaaaatccg cttaatcagt
     2881 agcgctgtct ggcaatataa acggcccctt ctg
//
LOCUS       DAZRUF010000328         2900 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZRUF010000328 DAZRUF010000000
VERSION     DAZRUF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.331"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..1199)
                     /locus_tag="ACHXOL_004054"
     CDS             complement(<1..1199)
                     /locus_tag="ACHXOL_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR6810887.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
     gene            1492..1586
                     /locus_tag="ACHXOL_004055"
     tRNA            1492..1586
                     /locus_tag="ACHXOL_004055"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Sec,seq:tca)
     gene            1858..2900
                     /locus_tag="ACHXOL_004056"
                     /pseudo
     CDS             1858..2900
                     /locus_tag="ACHXOL_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          845 a          691 c          665 g          699 t
ORIGIN      
        1 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
       61 agagcgccgc cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
      121 ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc
      181 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacata
      241 gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
      301 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagaggttt tgccagtgcg
      361 gaaccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgtg
      421 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
      481 ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa
      541 tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
      601 caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
      661 agtggtttat tatcaccgcc aattaaatta accagtggca tcatcagaac agtagaaagc
      721 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
      781 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgacggt ataaagtaag
      841 gtaagtaggg tgtaagtaat tgctgcatag atcattttgc cgttcatact gagatctggc
      901 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
      961 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     1021 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     1081 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     1141 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     1201 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     1261 gtgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     1321 ggtagcgtta attccgctca tatatcattg taaaatatgg gttttatatg agttataatg
     1381 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
     1441 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
     1501 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
     1561 ggttcgactc ctgtgatctt ccgccagttt acctcttctg aactctccca atgtcaataa
     1621 atctcttcta atcccttgta tatcagtatt tatttgtatc ctgaggtcaa cctacttcac
     1681 cccatatcaa gtttcttttg ggggtacttt agggggtgtt atttcctatt gaaatacaag
     1741 cacccccata ataggaaaaa tgtgatacat aacaacggct tttaacaata tctttgttat
     1801 acaaggggtg tattgggggt atcaggttag ttactggcag aaatccccct caggaacatg
     1861 aattcatcaa aagccggagg atgtcatggc attaaccgat atcaaagtga aaaccgcaaa
     1921 acccaaagac aaaccctata agcttgctga tggcggcggt atgtatctac tgattaacac
     1981 taatggttct aaatactggc gcatgaagta ccgctttgct ggtaaagaaa agatgctttc
     2041 tattggcgta taccccgatg taacgctagc cgatgctcgc gagaaacgca gtgaagccag
     2101 gaaactcctc gctgctggag gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca
     2161 gcaaatgagt ctgaaaaata cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc
     2221 tgatcggtgg tcattacgct atcgtgatga aatcattgat acctttgaaa aagacatttt
     2281 tccctacatt ggcaagcgcc ctattgccga aatcaaacca atggagttac tggaagcact
     2341 gcgtaaaatg gaaaaacgtg gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg
     2401 tgaggtgttc cgctatgcaa ttgttactgg ccgggcagac tataacccag cccccgatct
     2461 tgccagtgct ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact
     2521 tccccacttc ctcaacgatc tggcgggtta taccggaagt ataatcacta agacagctac
     2581 tcagatcatt atgttaaccg gcgtacgaac acaagaactg cgttttgcac gctgggaaga
     2641 tatcgatttt gagacaaaat tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc
     2701 tcatattgtg ccgctctctg agcaggtcat tatgctattt aagcagcttg aaccaatctc
     2761 aaaacatcat cccctagtct ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga
     2821 aagcattaac caagtaattg aattacttgg atataaggga agactcacag ggcacggttt
     2881 cagacacacc atgagtacaa
//
LOCUS       DAZRUF010000329         2883 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZRUF010000329 DAZRUF010000000
VERSION     DAZRUF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.332"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            125..757
                     /gene="comR"
                     /locus_tag="ACHXOL_004057"
     CDS             125..757
                     /gene="comR"
                     /locus_tag="ACHXOL_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR6810888.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(819..1358)
                     /locus_tag="ACHXOL_004058"
     CDS             complement(819..1358)
                     /locus_tag="ACHXOL_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR6810889.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1585..>2883)
                     /gene="ndh"
                     /locus_tag="ACHXOL_004059"
     CDS             complement(1585..>2883)
                     /gene="ndh"
                     /locus_tag="ACHXOL_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR6810890.1"
                     /translation="TPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHL
                     WKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKG
                     ELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYS
                     ANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERI
                     LPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKA
                     PDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQ
                     MATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIAR
                     FVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          699 a          779 c          702 g          703 t
ORIGIN      
        1 atttaaaaat ctttaaattt cagttaaata gaaagcaatt atttttatgg gatctggact
       61 ggtgaacgga ggtgattatt tttataataa cctttcattc acagtcacca ggttcaacgg
      121 catcatggca actgactcaa cacaatgtgt aaaaaaaagc cgtggccgcc caaaagtgtt
      181 cgacagggat gccgcgcttg ataaggccat gaaattgttc tggcaacacg gttatgaagc
      241 gacttctctt gcggacctcg tcgaagcgac cggagccaaa gcgcccacgc tatacgcgga
      301 atttaccaac aaagaggggt tatttcgtgc cgttctcgac cgctatatcg atcgttttgc
      361 cgctaagcat gaagcacagc tgttttgtga agagaaaagc gtggagtctg cgctggctga
      421 ctattttgct gccatcgcca actgctttac cagcaaagac accccggcag gctgcttcat
      481 gatcaacaac tgcaccaccc tctccccaga ttcaggagat atcgccaata cgttgaaatc
      541 acgccatgcg atgcaagagc gcactttgca gcagttttta tgtcaacgac aagcgcgcgg
      601 ggaaatcccg acccactgtg acgtgacaca tctggcagaa ttccttaatt gtattattca
      661 ggggatgtcg atcagcgcac gcgaaggtgc atcgctggaa aaactgatgc agattgccgg
      721 aacgactttg cgtttatggc ccgaactggt gaaataagtc gcaaaaaaag cccctcagcc
      781 tgagcgaatg aggggccaaa ttgcagagta cagccttgtt atactttgtt attcaaaatc
      841 agttgcccgt tactatctag cgggatctgc gtacccggat cgcggtccat gcggattttg
      901 ccctgctgat cgccaatctt ataggtcaca tcataaccga gcattttttc tgacttgtca
      961 tacaccgttt tacaacgctg ttgcgtagtc gtgtaagtat cgctttcctg gagagagccc
     1021 tggatctggt taccggcata tccaccaccc agcgccccca caacagtggc gacatcttta
     1081 ccacgaccac caccaaactg atgccctatc acgccgccag caacagcgcc gagcaccgac
     1141 ccggtaatgc gattttcatc ctgcaccggt cgacgatggg tcactgtgac gttgcgacac
     1201 tcctgacgcg gtgttttaac cgtttccttg attggggttg cagaaacaac ctgagcgtat
     1261 tgcgggcccc gttcaaacac gttcagactg gccactgccg ctacgcccag cgcagctgcg
     1321 acaccaatcc cgatacccgc caacattgat ttattcacgg gaacacctcc ttctttgcag
     1381 ttatgccaat catgcaagcg aaggagaaaa gcagctatca gacagaagat gaaaagcgcg
     1441 ggaatggctg gaaatactta aggattcgga gaagtctgaa ggaatgcctg atgcgacgct
     1501 taacgcgtct tatcaggcct accgctcgta ccatttgtcc agggcggatg aggcgtttat
     1561 gccacatccg ccagtgtacg tcgattaatg caacttcaaa cgcggacgga taacgcggtt
     1621 aatactcccc accagcatca ttaatccggt tttaaagtaa ccatgcagcg caatctgatg
     1681 cattcggtat agcgagatat atacaaagcg cgcaattcgt ccttcaatca tcattgagcc
     1741 gcgcgtcagg ttacccatca ggctaccgac agtggagaag ttcgacagcg ataccagcga
     1801 accatgatct ttatactgat aatttttcag cggtttaccg ttcatctgcg ccagaatatt
     1861 gttcattgcg caggtcgcca tctggtgtgc agcctgagca cgcggcggaa caaagccccc
     1921 ttccggacgc gggcatgacg cgcagtcgcc aatagcgtaa atgtctggat cgcgggtggt
     1981 ttgcagcgtc ggttccacca ccagctggtt gatacggttg gtttcaagac caccgatatc
     2041 tttcaggaag tctggcgctt tgatcccggc tgcccacacc atcagatcag cctcaatata
     2101 ttcaccatct ttagtgtgca ggccgccttc atcagcactg gtgaccatgg tttgcgtcag
     2161 cacgcgaacg ccaagtttcg ttagctcgtt gtgggccgca gcagagatac gtggcggtaa
     2221 tgcaggcaaa atacgttctc ccgcttctac cagcgttacg ttcagggctt cgttggtcag
     2281 gcctttgtaa ccgtagctgt gcagttgctt gaccgcattg tgcaattcag cggagagttc
     2341 tacacccgtc gcgccgccgc cgacaatcgc aatgttcact ttaccattcg cgcccaggtt
     2401 ggcggagtat ttcaggaaca gattcagcat ctcctggtgg aagcgacgcg cctggtgcgg
     2461 gttatcgagg aaaatgcagt tctctttgac acctggcgta ttgaaatcgt tagaggtgct
     2521 acccagcgcc attaccaggg tgtcataggc gattttacgt tccggaacca gcagttcacc
     2581 tttctcatcg cgcagttctg caatagtgat tgttttcgct tcacgatcaa tatcaatgac
     2641 ggaacccagc tggaactgga aaccatgatt gcgcgcatgg gccagatagc tcaacgcatc
     2701 gacgccttca tcaagcgagc cagtcgccac ttcgtgcagc agcggtttcc acagatggct
     2761 gtggttacga tcgaccagcg taattttggc ttttttcttg cgtcccagct tatgccccag
     2821 ctgtgttgcc atttccagcc caccagcacc gccgccgaca atcacaattt ttttcaatgg
     2881 cgt
//
LOCUS       DAZRUF010000330         2878 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZRUF010000330 DAZRUF010000000
VERSION     DAZRUF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.333"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHXOL_004060"
     CDS             complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHXOL_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR6810891.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGL"
     gene            1135..1653
                     /gene="mddA"
                     /locus_tag="ACHXOL_004061"
     CDS             1135..1653
                     /gene="mddA"
                     /locus_tag="ACHXOL_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR6810892.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1650..2099
                     /locus_tag="ACHXOL_004062"
     CDS             1650..2099
                     /locus_tag="ACHXOL_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR6810893.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHXOL_004063"
     CDS             complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHXOL_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR6810894.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHXOL_004064"
     CDS             complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHXOL_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR6810895.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2787..>2878
                     /gene="yncL"
                     /locus_tag="ACHXOL_004065"
     CDS             2787..>2878
                     /gene="yncL"
                     /locus_tag="ACHXOL_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR6810896.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          757 t
ORIGIN      
        1 taaaccatca gtaatatctt cgtggtagtg gattttatcc tctttcaccc attgccccat
       61 ctccttctga aactcatgga tgcggtgacc ataatcctga gcgataataa acccttgcaa
      121 gcgaatacgt tttttcagca ccgttgccat caacaggggt aaacgatccg gaccgggtgg
      181 tagctctgta gcgttatagc tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt
      241 aagtaatggt agcactgcat caaatacctt accgcccacg ttttcataat agatatcaat
      301 accttttggg cacgctttca ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca
      361 aacatcgaag cccagcacct cgatagcatg gcggcatttt tccgcgccac cggcaacccc
      421 caccaccctg caacctttaa gtttgccgat ttgccccacc gttgctccca caggacctgt
      481 cgccgcagct accaccaacg tttcgccctc tttaggctga ccgatatcca gcagccccat
      541 ataagcggta aagcctggca tccccagcac acccagcgac cacgatggat tttgcggatg
      601 atcgccaagt ttcaccagat catcaccact ggatatgtca tagtcctgcc atccactgta
      661 gcccagcacc cagtcgccag gctgataatc aggatgattc gactccacaa cacggctcac
      721 cgtgccgccg accatcacgc cgccaatatc aacaggcggt gaataagatg gctcatcgct
      781 catacgtcca cgcatatacg ggtccaggga caaataaact gtgcgcagta acacctgtcc
      841 ttcacccggt gtggcgacat catcttcttc aagacggaaa ttctccggaa ccggtgcgcc
      901 atgtggacgc gaggccagaa cccaacgtcg attacgctgc ttttgttgcc ccataatgct
      961 ctcctgtgct tttggcatga atccatcaat agtagcaggc tcacgggatt ggttttgttt
     1021 gcagccgtca catcccctct gcaacacgca ataatttcat cgaggaatcc gttgcttcgg
     1081 gctgttttcg atagtattga taacatcatc atcattattt tatcgaggtc agacatgtcc
     1141 atccgttttg cccgcaaagc cgattgtgct gccattgcgg aaatttataa ccacgccgtg
     1201 ttgtatacgg cggctatctg gaatgaccaa acggttgatg cagataaccg cattgcctgg
     1261 tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga tggcgtagtg
     1321 acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca taccgtggaa
     1381 cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc
     1441 cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg
     1501 caaaatcagg cctcgctgca tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg
     1561 ccgcaggtgg gcactaaatt tgggcgctgg ctggatctga catttatgca gttgcaactc
     1621 gacgaacgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt gcttttttga
     1681 ttgccgccgg aattggtctg gtagtccaga acacgctgat ggtgcgcatc acccagacct
     1741 cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg ctctttgttt
     1801 ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct
     1861 ggtggacact tatacccggc ttgctgggat cgttttttgt cttcgccagt atcagcggat
     1921 atcagaatgt gggagcggcg acaactattg cagtactggt cgccagccag ctaattggcg
     1981 gtctgatgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa
     2041 tttgtggcgc aattttactt gtggttggcg cctggttggt agccagacgc tcattttaat
     2101 cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc ttctttatgc
     2161 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
     2221 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
     2281 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
     2341 ccttaaccac ttgtgaaatt tatcgattca tccaaatgat ataagtctgc gtggtgccgt
     2401 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
     2461 ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     2521 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     2581 atagagagac atgtacgggc gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat
     2641 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     2701 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     2761 aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta gtactgataa
     2821 atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc tggtttat
//
LOCUS       DAZRUF010000331         2876 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZRUF010000331 DAZRUF010000000
VERSION     DAZRUF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.334"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHXOL_004066"
     CDS             complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHXOL_004066"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR6810897.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1555..>2876)
                     /gene="xylA"
                     /locus_tag="ACHXOL_004067"
     CDS             complement(1555..>2876)
                     /gene="xylA"
                     /locus_tag="ACHXOL_004067"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR6810898.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          685 a          844 c          669 g          678 t
ORIGIN      
        1 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      121 gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct ggattcgccg
      181 caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc gtacggtaat
      241 cgagctgctg accgctgata tccgccagca tctgacgcca gtactcacta cgcgccccgc
      301 caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca tccatgccat
      361 ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg gggccatgtt
      421 gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggt gtacgctcgc
      481 cggaaagata aggcagaaac caaactggct cgacactttc atcagcctgt tgagctgcag
      541 cgattaaagc tgggacattg cacaggccgg ttaatttcgc ggcccaatcc agacacgatg
      601 ctgcactcag cattacagac attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt
      661 gtacagcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag acccccgacg
      721 tccccagcga taacattgcc tgattagcat caaccattcc cacaccaact gcaccagctg
      781 cattgtcgcc accgcctgcg acaactggca ccgtcgccat accccacgct ttcgcaactt
      841 caggtaacaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc tggtcacgag
      901 ataagtcgca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca tccagccaca
      961 tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc agacgcaagt
     1021 aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca tgccgctgaa
     1081 cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta atcactcgtg
     1141 attgcggaac tctcgcttcc agcaaagtgc actcttgcgc acagcgcccg tcgttccaca
     1201 aaatggcagg gcgcaatacc cgttgttgag catccagtaa ggttgctcca tgcatctggc
     1261 cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga gctttcattg
     1321 cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt ggatgcgggc
     1381 gcgaaacggt cagcttttcc gtttgcgaat caaccacctc accctgctcg ttgagcaaaa
     1441 taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc gttccttaaa
     1501 aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta gccgttattt
     1561 gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac tctgatgcac
     1621 cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca tttggccttt
     1681 caggatttgc tggcccaatt cgctattcca gccggaataa cgctgcgcga tgcgtttatc
     1741 cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccagtgcca tcgtatccat
     1801 cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac gtactttggc
     1861 atcgaagttc agaccaccgg tggtgaaacc gcctgctttg agaatttcat acatcaccag
     1921 cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt gcgcatcgcc
     1981 acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcta tttcatgatg
     2041 gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa tctctttttc
     2101 cagaccaaac tgtttcagga agccatagac cgtcgcggca tcgtaatcat attgatgttt
     2161 agtcggttct tgcggtttcg gttcgataag caacgtgccc tggaagccga ttttatgttt
     2221 atgctcaacc accatctgca taaagcggcc cagttgttca cgctcctgac gcaagtcggt
     2281 atttaacagc gtttcgtaac cttcacgacc accccacagg acatagtttt caccgcccaa
     2341 tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccagctga agacttcagg
     2401 atctgggttc gtcgccgcac ccgcgccata gcgagggttt gtaaagcagt tagcggttcc
     2461 ccacagcagc ttcacgccgc tctcttcttg cttggctgcc aggacatcaa ccatttgcgc
     2521 aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca catcgtggaa
     2581 gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg ctttacgctt
     2641 cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc ccacaccaaa
     2701 catgtccgcc ccgttccagc agaaggtgtg ccagtagcag gcggcaaaac gcaagtgctc
     2761 ttccatacgc ttacccaaca ccagttcgtc gggattgtag tgacggaatg ctaacgggtt
     2821 tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg cttgca
//
LOCUS       DAZRUF010000332         2866 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZRUF010000332 DAZRUF010000000
VERSION     DAZRUF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.335"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            73..2451
                     /gene="ppsA"
                     /locus_tag="ACHXOL_004068"
     CDS             73..2451
                     /gene="ppsA"
                     /locus_tag="ACHXOL_004068"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR6810899.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2508..>2866)
                     /locus_tag="ACHXOL_004069"
     CDS             complement(2508..>2866)
                     /locus_tag="ACHXOL_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFR6810900.1"
                     /translation="GRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGE
                     RSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLR
                     KDIMRRLTQDVCEEIE"
BASE COUNT          673 a          719 c          792 g          682 t
ORIGIN      
        1 gttaaatatg caaagataaa tgcgcagaaa tgtgtttctc aaaccgttca tttatcacaa
       61 aaggattgtt cgatgtccaa caatggctcg tcaccgctgg tgctttggta taaccaactc
      121 ggcatgaatg atgtagacag ggttgggggc aaaaatgcct ccctgggtga aatgattact
      181 aacctttccg gaatgggtgt ttccgttccg aatggtttcg ccacaaccgc cgacgcgttt
      241 aaccagtttc tggaccaaag cggcgtaaac cagcgcattt atgaactgct ggataaaacg
      301 gatattgacg atgttactca gcttgcgaaa gcgggcgcgc aaatccgcca gtggattatc
      361 gacactccct tccagcctga gctggaaaac gccatccgcg aagcctatgc acagctttcc
      421 gccgatgacg aaaacgcctc ttttgcggtg cgctcctccg ccaccgcaga agatatgccg
      481 gacgcttctt ttgccggtca gcaggaaacc ttcctcaacg ttcagggttt tgacgccgtt
      541 ctcgtggcag tgaagcatgt atttgcttct ctgtttaacg atcgcgccat ctcttatcgt
      601 gtgcaccagg gttacgatca ccgtggcgtg gcgctctctg ccggtgttca gcggatggtg
      661 cgctccgacc tcgcatcttc tggcgtgatg ttctccattg ataccgaatc cggctttgac
      721 caggtggtgt ttatcacttc cgcatggggc cttggggaaa tggtcgtgca gggtgcggtt
      781 aacccggatg agttttacgt ccataaaccg acactggcgg cgaatcgccc ggctatcgtg
      841 cgtcgcacca tggggtcgaa aaaaatccgc atggtttacg cgccgaccca ggagcacggc
      901 aagcaggtta aaatcgaaga cgtaccgcag gaacagcgtg acatcttctc gctgaccaac
      961 gaagaagtgc aggaactggc aaaacaggcc gtacaaattg agaaacacta cggtcgcccg
     1021 atggatattg agtgggcgaa agatggccac actggcaaac tgttcattgt gcaggcgcgt
     1081 ccggaaaccg tgcgctcacg cggtcaggtc atggagcgtt atacgctgca ttcacagggt
     1141 aagattatcg ccgaaggccg tgctatcggt catcgcatcg gcgcgggtcc ggtgaaagtc
     1201 atccatgaga tcagcgaaat gaaccgcatc gaacctggtg acgtgctggt cactgacatg
     1261 accgacccgg actgggaacc gatcatgaag aaagcatctg ccatcgtcac caaccgtggc
     1321 ggtcgtacct gtcacgcggc gatcatcgct cgtgaactgg gcattccggc ggtagtgggc
     1381 tgtggtgatg caacagaacg gatgaaagac ggtgagaacg tcactgtttc ttgtgccgaa
     1441 ggtgataccg gttacgtcta tgcggagttg ctggaattta gcgtgaaaag ctccagcgta
     1501 gaaatgatgc cggatctgcc gttgaaagtg atgatgaacg tcggtaaccc ggaccgtgct
     1561 ttcgacttcg cctgcctgcc gaacgaaggc gtgggccttg cgcgtctgga atttatcatc
     1621 aaccgtatga ttggcgtcca cccacgcgca ctgcttgagt ttgacgatca ggaaccgcag
     1681 ttgcaaaatg aaatccgcga gatgatgaaa ggttttgatt ctccgcgtga attttacgtt
     1741 ggtcgtctga ctgaagggat cgcgacgctg ggtgccgcgt tttatccgaa gcgcgtcatt
     1801 gtccgtctct ctgattttaa atcgaacgaa tatgccaacc tggtcggtgg tgagcgttac
     1861 gagccagatg aagagaaccc gatgctcggc ttccgtggcg cgggacgcta tgtttccgac
     1921 agcttccgcg actgcttcgc gctggagtgc gaagcagtga aacgtgtgcg caacgacatg
     1981 gggctgacca acgttgagat catgatcccg ttcgtgcgta ccgtagatca ggcgaaagcg
     2041 gtggttgagg aactggcgcg tcaggggctg aagcgtggtg agaacgggct gaaaatcatc
     2101 atgatgtgtg aaatcccgtc caacgccttg ctggccgagc agttcctcga atatttcgac
     2161 ggcttctcaa ttggctcaaa cgatatgacg cagctggcgc tcggtctgga ccgtgactcc
     2221 ggcgtggtgt ctgaattgtt cgatgagcgc aacgatgcgg tgaaagcact gctgtcgatg
     2281 gctatccgtg ccgcgaagaa acagggcaaa tatgtcggga tttgcggtca gggtccgtcc
     2341 gaccacgaag actttgccgc atggttgatg gaagagggga tcgatagcct gtctctgaac
     2401 ccggacaccg tggtgcaaac ctggttaagc ttggctgaac tgaagaaata aaataaatcc
     2461 ccggcggcgt tcagtcgccg gggttatgtg atccccgaag atgaaactta ttcaatctct
     2521 tcacagacat cctgagttaa ccgccgcata atatcttttc ttaacaaaaa cttttgtatt
     2581 ttacctgagg cagtacgcgg tagtttttcg attaccacga tatgttcagg atatttatat
     2641 tttgcgaccc gtttacggct aaaaaaagcc accacctctt ccagcgataa tgaatgatgc
     2701 ggcgctttca gcacgacata agcgcatgat cgttcaccta aacgttcatc gggcattgca
     2761 accacacagg catcgtgaat tttaggatgc tgcaataaaa tatcttctac ttcacggctg
     2821 ctaatatttt cgccgccgcg gacaataata tcttttttgc gtccgg
//
LOCUS       DAZRUF010000333         2846 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZRUF010000333 DAZRUF010000000
VERSION     DAZRUF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.336"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..557
                     /locus_tag="ACHXOL_004070"
     CDS             <1..557
                     /locus_tag="ACHXOL_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810901.1"
                     /translation="APQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGTLKN
                     YYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDAQIL
                     MFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLPDEK
                     VREIYALSEAKGDHLNLPQKAASA"
     gene            complement(1039..1659)
                     /gene="fklB"
                     /locus_tag="ACHXOL_004071"
     CDS             complement(1039..1659)
                     /gene="fklB"
                     /locus_tag="ACHXOL_004071"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR6810902.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            1878..2516
                     /gene="ytfB"
                     /locus_tag="ACHXOL_004072"
     CDS             1878..2516
                     /gene="ytfB"
                     /locus_tag="ACHXOL_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR6810903.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(2500..>2846)
                     /locus_tag="ACHXOL_004073"
     CDS             complement(2500..>2846)
                     /locus_tag="ACHXOL_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR6810904.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
BASE COUNT          782 a          676 c          684 g          704 t
ORIGIN      
        1 aagcacccca aggttctttt cctgccgatg gaaaagaacc gattatcgaa agaatcttta
       61 ggttagtaga acgataccct tcaagaaatg cggctgctcg ggcatggggg atcaatgaag
      121 gcactttaaa aaattattac aacaggcggg attacgcacc gataccacgc ccgcatcagt
      181 taaaaaagat agcagaaagc gaaaatgtta ccttggaatg gctcatgacg ggagagggtg
      241 atgttgaact aaaagattcc aaagaaccca aaaaagagaa tgcggataat gttgatgctc
      301 aaattctaat gttcttgtca tttcttaaac cacaagaaaa acaacaagta gctgatgttc
      361 ttgggagaaa aggagcggag caattactca ttcttctgga tgagcacatc caagagttgc
      421 acgctctagt tggagttcgt agagccatag cgttaagcct tggtaatttg ccagacgaaa
      481 aggttagaga gatttacgcg ttgtccgaag ctaagggcga tcacttaaat ctacctcaga
      541 aagcagccag cgcataaccc agtgcaagtg cctagctgtg ctaaaattac attggttttg
      601 gcgcttgcac ccatcagcgc tataataaac acctgaatcg tttttgaacg gtctttaaag
      661 gctattgtaa gaacctgtga gttatcttga atgttttatt gatatcgaac cgtttaacat
      721 ttttggactc gctttatctt gaaaaccttg agttcctaac ttgttgattc caatctattc
      781 ccgtcttttt taatctcttc ccggttatct tgaataaatt acctagtcat acaatacgcg
      841 ggatgaatac taagatttag atctaatgtt aattttatgt ttaaagtgag tgtcatatca
      901 cggtgataac gcgaaacagc tcacaaaaat acacgcttac ggggtctttg caacacaaaa
      961 tagcgccgtt tggcaaactg aagggtttat tgctgaatgc ctgctcccct ctggttcgag
     1021 gggaacagaa tgcgctgctt agaggatttc cagcagttcg acttcaaaca ccagggtgct
     1081 gaacggaggg atggatgcgc ctgcgccgcg ctcgccatat gccagttcct gcgggatagt
     1141 cagttcccat ttagaaccta ccggcatcag agtcagtgct tcaatccagc cagggatcac
     1201 gccattaacc gggaattcag cgggttcacc acgagcaacg gagctgtcaa acacggtgcc
     1261 gtcgatcagt ttaccggtgt aatgaacacg aacgcggtcg gtacgtgccg gaattgcgcc
     1321 ttcaccctgg ttgatcacgc ggaattgcag gccagattcg gtgctattca caccttcttt
     1381 tttggcgttt tcttccaggt atttcacacc ttcagcagcc atcgcctgga aacgctgacg
     1441 acgaacggca tcggcgcgtt cgtggatttc acgcagcgcg cgatgcacca catcaaccgg
     1501 cacagccgga tgtttgcctt ccagcgcatc ggcaatacct gcaaccagtg cttctggcag
     1561 cagcccttcc aggccagatt cactcagttg ttgccctacc tgcaaaccaa tgccgtagct
     1621 tgcttgcgct tcgatggtgt caaaagttgg ggtggtcatc gtatttcctt tcttagggta
     1681 taaaagtagc gggcagcata tcagccacac ttctttgggt aaaactttgt cgcaggaatg
     1741 atgacaaatc ccggggaaaa agaaacaata gcaatatccc gtatgaatat aataaaggcg
     1801 ggtttaccac ggacatcata gcgttagctt attcataggc tatgattgag gaacaagacg
     1861 cggagcagga ggaaaccatg cccgggcgct ttgaactaaa accaaccctg gagaaagtct
     1921 ggcacgcgcc ggacaatttt cgcttcatgg acccgctgcc gccgatgcat cgtcggggga
     1981 tcattattgc cgccattgta ttggtggtcg gatttctgct tccatctgat gatacgccca
     2041 acgcgccggt ggtgacgcgt gaagcgcagt tagatattca gtcgcaatca caaccgccga
     2101 cagaagaaca gctgcgggcg caattagtca caccacaaaa tgatcctgat caggtggctc
     2161 cggtcgctcc agaacctata caggaaggcc agccagaaga acagccgcaa actacccaaa
     2221 cgcaaccatt ccagccagat agcggaattg ataatcaatg gcgctcttac cgtgtcgaac
     2281 ccggtaaaac gatggcgcag ctgttccgcg accacggact gcccgctacc gatgtctatg
     2341 caatggcgca ggtagaaggc gcgggtaagc cgctgagtaa tttgcaaaat gggcagatgg
     2401 tgaaaatccg ccagaacgcc agcggcgtgg tgacgggctt aaccattgat acggggaata
     2461 atcaacaggt cctgtttacc cgccagccgg atggcagttt tattcgtgcg cggtagatga
     2521 agggtcaacc aaaagccagc taaggattgt ctctttgatg accttacgtc tggcttccag
     2581 tgcctggggt gaaaccatac tgatgtgata aaccaggcca agcgtatgct ggttgatcaa
     2641 ataatatccc ccaagcgcag caatattaat attcacctgc aatgggtcga tatctggctt
     2701 aaaaatattg tgtttcttcc cttcatccaa taaggattcc attaactgca agtgcgcatg
     2761 gttaatttct aagagacgca gtgattttgc ataatgcaca cctttacttt gattttcact
     2821 atgcacaatt tttaaaaacc aagggt
//
LOCUS       DAZRUF010000334         2840 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZRUF010000334 DAZRUF010000000
VERSION     DAZRUF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.337"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            405..1976
                     /gene="garD"
                     /locus_tag="ACHXOL_004074"
     CDS             405..1976
                     /gene="garD"
                     /locus_tag="ACHXOL_004074"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR6810905.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            2125..2509
                     /locus_tag="ACHXOL_004075"
                     /pseudo
     CDS             2125..2509
                     /locus_tag="ACHXOL_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
     gene            2509..>2840
                     /gene="yhaV"
                     /locus_tag="ACHXOL_004076"
     CDS             2509..>2840
                     /gene="yhaV"
                     /locus_tag="ACHXOL_004076"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR6810906.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          769 a          644 c          716 g          711 t
ORIGIN      
        1 cttttttacg tcaacggtgt ccagaatcat tttatttacc tcgggtactt atgctgattt
       61 ttattattat ggggaaggtg ttatttatga gttttattta tgccgttacg acaatgaact
      121 cgggaattag tataagcagc gggagaataa taatcattgt gcaaatgcta atttaatcag
      181 tactatttaa atattatttt gagcatatgc acataaggtt gcgcgctaaa gcacagattt
      241 gcgctttacc ttaccgggcg gcactgcaat ccctgaaatg attgacattg atcacatttc
      301 tgcgtttaaa ctcctgacat tcttatttca cccaatgaag tcatttattt ttaaatgaga
      361 ccagatcctc attttaataa cccctggctg gagaatattg cacaatggcc aacatcgaaa
      421 tcagacaaga aacgccaact gcgttttata taaaagttca cgacacagat aatgtggcaa
      481 ttattgttaa tgataatggc ctgaaagcag gaacgcgttt tccggatggg ctggaattaa
      541 ttgaacatat tccccagggg cataaagtcg cattgctgga cattccagct aatggtgaaa
      601 ttattcgtta tggcgaagtg attggttacg ccgtgcgcgc aatcccacgc ggaagctgga
      661 tcgacgaatc aatggttgta ctaccggaag cgccgccgtt acacacgctg ccactggcaa
      721 ccaaagtccc ggccccctta ccgccgctgg aaggatacac ctttgagggc tatcgcaatg
      781 ccgatggcag cgtgggcacc aaaaacctgc tcggcatcac caccagcgtc cactgtgtgg
      841 caggcgtggt ggactatgta gtaaaaatca ttgaacgcga tctgctaccg aaatacccga
      901 acgtcgatgg cgtggtgggg ctgaatcatt tgtacggctg tggcgtggcg attaacgcac
      961 cggcggcagt tgtgcctatt cgtaccatcc acaatatttc gctgaaccct aactttggcg
     1021 gcgaagtaat ggtgattggt ctgggttgtg aaaagttgca gcctgagcgc ctgctggtcg
     1081 gcacggatga tgtgcaagct attccagtag aaagcgccag cattgtcagt ttgcaggatg
     1141 aaaagcatgt cggctttcag tccatggtcg aggatatttt gcaggtcgcc gaacgccatc
     1201 tacaaaaact gaatcaacgg cagcgagaaa cctgcccggc ttcagaactg gtcgttggtg
     1261 tgcagtgcgg tggcagcgat gcgttttctg gtgtaacggc aaacccggcg gttggctatg
     1321 cgtctgatct actggtgcgc tgcggcgcaa cggtgatgtt ttcagaagta acggaagtgc
     1381 gtgacgcgat ccatctgctg acaccacgcg cagtgaacga agaggtcggc aaacggctgc
     1441 tggaggagat ggagtggtac gataactatc tcaatatggg aaaaaccgac cgcagcgcca
     1501 acccttcgcc gggcaacaag aaaggcggtc tggcaaatgt ggtggagaag gcactcggct
     1561 ccattgctaa atcgggtaaa agtgcaattg ttgaagtgct gtcgcccggt caacgcccga
     1621 ctaaacgcgg attaatttac gccgcgacgc cagccagcga ttttgtctgt ggcacgcaac
     1681 aggtggcttc gggtatcacc gtacaagtgt ttacgaccgg ccgtggtacg ccgtacggcc
     1741 tgatggcggt acccgtaatt aaaatggcaa cccgcaccga gctggcgaac cgctggtttg
     1801 atttaatgga tattaacgcg ggcactatcg ctaccggcga agagagcatt gaagaggtgg
     1861 gctggaagtt gttccacttt attctcgacg tcgccagcgg gaagaagaaa accttctcgg
     1921 atcaatgggg gctgcataac cagctggcgg tgtttaaccc ggcaccggtg acctgatttc
     1981 ttttggaacg tccctcgcaa aacatggcct tagtgccatg tttttattgt ttaaagcccc
     2041 cacgtccatt aataatgcat ttgcattacc ttagttcaag cttataattt gagcagaaaa
     2101 caggctgtaa aaggacaatg aatcatgccc gctaatgctc gctctcacgc tgtactgacc
     2161 actgaatcaa aggtcacgat acgcggacaa acaactatcc ccgcgccagt gcgtgaggcc
     2221 ttaaaactga agccaggcca ggacagcatt cattacgaaa ttctgcttgg tgggcaagta
     2281 tttatgtgcc gactgggaga tgaacaggag gatcatacta tgaatgcatt tttgcgtttt
     2341 ctggatacag atatccagaa caacccgcaa aaaactcgtc cattcaacat tcaacattca
     2401 acattcaaca ttcaacattc aacattcaac attcaacatt caacaaggaa agaaacttgt
     2461 cgctggcatg gacgtcaaca ttgatgatga gattggcgac gacgaataat ggattttcca
     2521 caaagggtta atggttgggc gctatatgct catccctgtt ttcaggaaac ctacgacgct
     2581 ttagttgccg aagtcgagac attaaaggga aaagatcctg aaaattatca gagaaaagcc
     2641 gccacaaagt tattggcggt agtccataaa gtgattgagg agcatatcac ggtcaatcca
     2701 tcatcaccgg cattccgtca tggcaagtcg ttaggctctg ggaaaaataa agactggtca
     2761 cgggtaaaat ttggtgcagg tcgttatcgt ctcttctttc gttatagtga aaaagagaaa
     2821 gtcatcattc tgggatggat
//
LOCUS       DAZRUF010000335         2829 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZRUF010000335 DAZRUF010000000
VERSION     DAZRUF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.338"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            69..1010
                     /locus_tag="ACHXOL_004077"
                     /pseudo
     CDS             69..1010
                     /locus_tag="ACHXOL_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            1010..1108
                     /locus_tag="ACHXOL_004078"
                     /pseudo
     CDS             1010..1108
                     /locus_tag="ACHXOL_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1108..2429
                     /gene="hipA"
                     /locus_tag="ACHXOL_004079"
                     /pseudo
     CDS             1108..2429
                     /gene="hipA"
                     /locus_tag="ACHXOL_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2613..2714
                     /locus_tag="ACHXOL_004080"
                     /pseudo
     CDS             2613..2714
                     /locus_tag="ACHXOL_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          772 a          558 c          743 g          756 t
ORIGIN      
        1 taggtgggca ttgcacatat ttccgacgaa tagatcacaa tttatgctat tttgattttc
       61 acggttgcac atcaacagtt aatttgcgta aatcctatgg actgacaacg aaattcagta
      121 gattgacgct gggtgttttg ctggcactaa gcggttcagc gtctggtgcg agtctggaag
      181 ttgataatgg tcagattacc aatattgata ctgatgttgc ttatgatgcc tacctggttg
      241 gctggtatgg cactggagtt cttaatattt tggctgacgg taatgcctac ttaaccacta
      301 ttactaccag cgtcattggc gctaatgagg attcggaggg taccgttaat gttttgggtg
      361 gcacctggcg attgtatgat agcggaaata atgcaaggcc tttaaatgtg ggtcaatccg
      421 gaacggggac gctgaatatt aaacagaaag gtcacgttga tggaggctat ttaagactag
      481 gttcctcgac aggaggcgtc gggacggtca atgttgaggg agaagactct gttctgacga
      541 ccgaattatt cgaaataggg agttacggta cgggctcatt aaatattacg gataagggtt
      601 acgtcacgag ttcaatagtc gccattttag gatatcaagc gaacagtaat ggtaaggtta
      661 tcgttgaaaa gggtggcgag tggctaataa aaaataatga ttcctcaatt gaatttcaaa
      721 ttggtaatca aggagctggg gaggcgacta ttcgcgaggg tgggctgatt acggctgaaa
      781 atacgattat cggtggcaat gccaccggta tcggaaccct gaatgtgcag gatcaagact
      841 ctgtcatcac ggtacgcaga ctctataatg ggtatttcgg taatggcaca gtcaatattt
      901 ccaataatgg tctgattaat aacaaagaat attcattggt gggcgttcag gacggttccc
      961 acggtgtcgt caacgtgacc gataaagggc attggagttt cctcggaact tttttaagat
     1021 tttacagtcg cttgaactct caatgacgct atgcgacgcg aaaaatgcct cgccagaagc
     1081 aacagaacag caagatctgg agtggtaatg cctaaacttg tcacttggat gaacaaccag
     1141 cgggtaggcg agttaacgaa gttagccaac ggcgcgcaca cctttaagta tgcaccggag
     1201 tggttagcca gccgttatgc cagaccgttg tcactttcgc tgccattgca gagggggaat
     1261 atcacctctg atgccgtatt taacttcttc gataacctgt tacccgatag cccgattgta
     1321 cgtgaccgga tcgttaaacg ttatcatgcc aaatccagac aaccgtttga tttgttgtca
     1381 gaaatagggc gagacagcgt tggtgccgtg acgttaatac ccgaagacga aaccgtaacg
     1441 cgtccgataa tggcatggga aaagcttact gaagccagac ttgaagaagt attaacggct
     1501 tataaagcag atatcccgct aggcatgatt agagaagaaa atgactttcg catctcggtt
     1561 gctggcgcac aggagaagac agcactgctc agaataggca atgactggtg cattccgaaa
     1621 ggaataacgc cgacgacgca catcattaaa ttaccgattg gcgaaatcag gcagcccaat
     1681 gcgacgctcg atctcagcca aagcgttgat aatgagtatt actgtctgct gctggcgaaa
     1741 gaacttgggt tgaatgttcc ggacgcagaa atcattaaag cgggaagggt gcacgcgtta
     1801 gcggtcgaac gttttgacag gcgttggaat actgagcgaa cggttttact tcgcttgcca
     1861 caggaggata tgtgtcagac attcggttta ccttcatcgg tgaaatatga atcagatgga
     1921 ggcccaggca tcgcgcggat catggcgttt ttgatggggt ccagcgaggc gctgaaagat
     1981 cgctatgatt ttatgaagtt tcaagtgttc cagtggttga ttggcgcaac ggatggccat
     2041 gcaaaaaact tctccgtatt tattcaggct ggcggcagtt atcgactcac gccattttac
     2101 gacatcattt cagcatttcc ggtccttggc ggtacgggaa taacatcagc gatctcaaac
     2161 tggcaatggg gcttaacgca tccaaaggca aaaaaacggc aatcgataaa atttatccgc
     2221 gacatttttt ggcgacagca aaggtgctga gattcccgga agtgcagatg catgaaatcc
     2281 taagtgactt tgccagaatg attccggcag cactggataa cgtgaagact tcattaccga
     2341 cagattttcc ggagaacgtg gtgacggcag ttgaaaccaa tgtgttgagg ttgcatggac
     2401 ggttaagccg agaatacggt agtaagtgag atatggggtt tggtcgttgt taatgagcaa
     2461 tcatgaccgg ccaacgactg gattctccct tggtttatgt ttgtgcgcat aatgtatatt
     2521 atgttaaatc atgtctgagt gatgcgaagt gcttttctgg tagtcgttat tcgttcagat
     2581 taggtgtcct tgtgatttaa agatgttatc gtggcacgaa tatactgcca ttccaggctc
     2641 gttatattgc tctcgggcag tccgtagctg gtactgcaaa cgctgatgcg accttcaaag
     2701 ttcaatatct ataattcaat cttaaggtgg ctatacgtca tctgaaattg aaacagggtg
     2761 tgtgtggcat ggctacgcac acccgagcaa ttcaccaatg tgaatctcat tattaatgag
     2821 gagtggttt
//
LOCUS       DAZRUF010000336         2823 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZRUF010000336 DAZRUF010000000
VERSION     DAZRUF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.339"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="ACHXOL_004081"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="ACHXOL_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="ACHXOL_004082"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="ACHXOL_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR6810907.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="ACHXOL_004083"
     CDS             2354..2758
                     /locus_tag="ACHXOL_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFR6810908.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
BASE COUNT          835 a          593 c          614 g          781 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgccg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcgc ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aag
//
LOCUS       DAZRUF010000337         2788 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZRUF010000337 DAZRUF010000000
VERSION     DAZRUF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.340"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(11..79)
                     /locus_tag="ACHXOL_004084"
                     /pseudo
     CDS             complement(11..79)
                     /locus_tag="ACHXOL_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310734.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            101..514
                     /gene="hiuH"
                     /locus_tag="ACHXOL_004085"
     CDS             101..514
                     /gene="hiuH"
                     /locus_tag="ACHXOL_004085"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR6810909.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            623..1627
                     /gene="msrP"
                     /locus_tag="ACHXOL_004086"
     CDS             623..1627
                     /gene="msrP"
                     /locus_tag="ACHXOL_004086"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR6810910.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            1628..2263
                     /gene="msrQ"
                     /locus_tag="ACHXOL_004087"
     CDS             1628..2263
                     /gene="msrQ"
                     /locus_tag="ACHXOL_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR6810911.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            2520..>2788
                     /locus_tag="ACHXOL_004088"
     CDS             2520..>2788
                     /locus_tag="ACHXOL_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFR6810912.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKT"
BASE COUNT          732 a          668 c          645 g          743 t
ORIGIN      
        1 aaacagccta gttcattaca aaattgtaat gctgctgtaa ggttaccctg gccgcttttt
       61 cgctatcctc aaaactcatt cacatgacaa ggatataaac atgttaaagc gttatttagt
      121 actctccgta gtaacggcag cattttcatt accttctttg gtttatgccg cacaacaaaa
      181 cattcttagc gtgcacattt tgaaccagca aaccggaaaa cccgctgccg acgtgacagt
      241 cactcttgaa aagaaagcgg acaacggctg gttacaactt aataccgcca aaacagataa
      301 ggatggacga attaaggcac tgtggcccga gcaaactgca actacgggcg attaccgtgt
      361 cgtatttaaa accggggact atttcaagaa acaaaatctt gaaagtttct tccctgagat
      421 ccccgttgaa tttcatatta ataaagtgaa cgagcattat catgtgcctt tattacttag
      481 ccaatatggg tattcaacct atcgtggcag ttaatgcata aatatgtaaa agcagatctc
      541 tgctactgtt agggaggaca ctccctgaca gattaacagt aaacggctct tgctggctaa
      601 cgacaaaaaa gtgtgatggc ttatgaaaaa gaatcaattt ttaaaagaat cagatgttac
      661 ggccgagtcg gtattcttta tgaagcgtcg acaggtgtta aaagcactgg gcatcagcgc
      721 agctgcactt tctttgcctc acgctgcgca tgccgatctg cttagctggt ttaaagggaa
      781 cgatcgcccg cccgcccccg ccggaaaacc gctggagttc agcaagccta ccgcctggca
      841 aaataatctg ccactgacgc cagtagataa agtctccggt tataacaact tctatgaatt
      901 cgggctggat aaagccgatc ccgccgctaa tgctggtagc ctgaaaaccg atccatggac
      961 actgaaaatc agcggcgaag tggcaaaacc attgaccctc gatcacgatg atttaacccg
     1021 tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc gtggaggcat ggtcgatggt
     1081 ggtgccgtgg attggttttc cgctgcacaa attgctggcg cttgccgaac ccaccagcaa
     1141 tgcgaagtat gtcgctttcg aaacaattta tgcaccggaa caaatgccag gccagcagga
     1201 ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa ggattgcgtc tcgacgaagc
     1261 aatgcatccg ctcacactga tgaccgtggg tgtttatggc aaggcgttac cgccacaaaa
     1321 tggcgcgccg gtacgactga ttgtgccgtg gaaatatggc tttaaaggga ttaaatcgat
     1381 agtcagtatt aagctgaccc gcgagcgtcc gccaaccacc tggaatctgg cagcgcctga
     1441 cgaatacggt ttttacgcca acgttaatcc gcatgttgat cacccgcgct ggtcacaggc
     1501 taccgaacga tttattggtt caggcggcat cctcgatgtt cagcgccagc caacgctact
     1561 gtttaatggt tacgccgacc aggtggcatc gctgtatcgt ggcctggatt tgcgggagaa
     1621 tttctgaatg cgtctgacag caaaacaggt gacatggctg aaagttagcc tgcatcttgc
     1681 cggattgttg ccgtttctct ggctggtctg ggcgatcaat cacggtggac tgggtgccga
     1741 tccggtgaaa gatattcagc attttaccgg tcgcactgca ctgaaatttt tgctggcgac
     1801 cttgttaatc acacctctgg cacgctacgc aaaacagccg ttattgatac gcactcgccg
     1861 cctgttaggc ttatggtgct ttgcctgggc gacactgcat ttaaccagtt acgcattgct
     1921 ggagctgggc gtgaataatc tggcgttatt aggcaaggag ttaattaccc ggccttattt
     1981 aacgttagga attatcagct gggttatttt gcttgcttta gcgtttactt caacccaggc
     2041 gatgcagcga aaactcggca agcactggca acagttgcat aatttcgtct atcttgtcgc
     2101 gatcctggcc ccaatacatt atctgtggtc agtgaagatt atttcaccgc agccgctcat
     2161 ctacgctggg ctggctgtac tgcttttagc cttacggtat aagaagttgc gttccctttt
     2221 taagcggtta cgcaagcagg ttcacaacaa attatctgta taacttcata ttgccgacaa
     2281 agtacgcgtt atccctgccg gatgtggcgc gagcgccctg tccggcctgc gcagatcaca
     2341 tcaggttatg tttcgtttgt catcaacctc aaattatcaa cataattcat tttatttcaa
     2401 taagatagat aagtacacct gagaaaacta tgctctcatc tcctaagagt tgttgcattt
     2461 tgccatatgt tacaatataa cattacacat catatacaat gactctggag gaaactgttt
     2521 tggcaattcg tcttcacaaa ctggctgttg ctttaggtgt ctttattgtt agcgctcctg
     2581 ccttttcgca tggtcatcac tcacacggca aacccttaac agaggtcgaa caaaaagctg
     2641 ctaatggtgt ttttgatgat gccaatgtac aaaaccgaac gctcagtgac tgggatggag
     2701 tctggcaatc cgtttatcct ttactgcaaa gtggcaaact tgaccccgtc tttcagaaga
     2761 aagcggatgc agataaaatt aaaacatt
//
LOCUS       DAZRUF010000338         2732 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZRUF010000338 DAZRUF010000000
VERSION     DAZRUF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.341"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(108..1472)
                     /locus_tag="ACHXOL_004089"
                     /pseudo
     CDS             complement(108..1472)
                     /locus_tag="ACHXOL_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(1537..1656)
                     /locus_tag="ACHXOL_004090"
     CDS             complement(1537..1656)
                     /locus_tag="ACHXOL_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR6810913.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1880..2038)
                     /locus_tag="ACHXOL_004091"
                     /pseudo
     CDS             complement(1880..2038)
                     /locus_tag="ACHXOL_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2383..>2732)
                     /locus_tag="ACHXOL_004092"
     CDS             complement(2383..>2732)
                     /locus_tag="ACHXOL_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810914.1"
                     /translation="DFKGCFFSGQNFSNYDIQYVNWGTSLFDLDTPCIFNAPAYNKSN
                     EKSLKPVSENGLSGVLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSEDDPVENSPVV
                     TSPVVSSSKSSFQ"
BASE COUNT          745 a          649 c          514 g          824 t
ORIGIN      
        1 cctaagacat caatcatctg ctctccaatg actagtctaa aaactagtat taagactatc
       61 acttaaataa gtgatactgg ttgtctggag attcaggggg ccagtctagc aactaaagca
      121 ctgcccgtct cctggttaac cccatgtgct ataccgccgg tatagacaat cagcgagcct
      181 ccggcgctaa tattgctgcc agttgccgta ccatctttct caacaacctg ccggctcccg
      241 gtggaaatat ttgtcgtgtc agctttcccg ccgctcttga tattttgtgt tccgctgttg
      301 atattggtac ccgtggcaat accatgatta ttaatattct gtgtaccacc attgattatg
      361 gtattggtcg cgtttctggt aacatccatt accccgccgt tatctattcg ggtcgcatca
      421 gctttagcat tcgttaaaac tgacattgtt cctttatctt taataatcgt cttatttgcc
      481 gaaccatatg cgtttatgtc taaatgacca ccgttttcca gcaacacatt gtctgccacg
      541 tgattgtgga tggagaatgc accttcacta ttcgtaccgc tcaccgtcgt accgttagtg
      601 tttgttttta aaattgcacc atcgtgctgg gtaacatttg ttgccgtacc accactaaca
      661 tcaagcacgc caccggaata aacttcaata acatccgagg agctggtgta atcaacaatt
      721 tgcgtaccac cagaatagat ctgagtattt tttgccgttg acttattatt cagagactga
      781 gttccacctt caatcgtcgt gtccagcgca tggctttcat atactctttg ctcaccgcca
      841 tttttaatgg tggttgttcc tactgtgctc tgttcaacat actgtcgtcc accatttatg
      901 attgtgttcg ttgccatact tccttgcgtg atgtcctgat aaccggactt atttatcgtt
      961 gtaccatcaa catgcccctg agttgttact gtctgccctc caccatcaat gatggttcca
     1021 ttcgcagtcc ccccacttat gtgtgaatta ccacccttaa ttgtcgttcc attgctgata
     1081 ccacctgtat aaacgtcctg attgccactg tcgattatcg taccggtgga aatgcccccg
     1141 tcatgaattg tctgtcttcc tccgttaatg gttgtattat tagactgccc catataattg
     1201 ttattccttc caacatcttg atatccacca gattcaataa gacttccatt agatactccg
     1261 ccataaacac tctgctggcc atggttgata atatgagtgt tatttgttgt acctcgttca
     1321 tctactcgtt ggttgccatc tacagtctca tcgtttacca caccaataac attagtagta
     1381 aaggccgcca tcacgggcgg ggcatatatc aaggcagata tcaataagaa aagtactgag
     1441 cggcgacaat aatggggact ggtcctgttc ataaatttca tcctctgaaa agtgaatact
     1501 gagtagcgtt taagcgactt tagctctgct gcaacatcag cccgcaggca ccagaccggg
     1561 gggttcatcc tgaagagaca gcgcaagtgt attgtgttca ccgctcatca aagacatcat
     1621 gatgaaatga tgatgttcca cataagaaag aggcattttt taaacacagt gcgctgaagt
     1681 gtgattggat aaaaaagtca atctattcag gaaatacggg cgtattcttt tctttcgaca
     1741 atcaggccgt cggcaaaata aaatgattta cataatcgtt tctgatgaat atcttctgct
     1801 cacataaaaa tcacacaata actttgagat cgcagattct tttactttta cagcattcgt
     1861 ccccccattg ttgggcaaat atagattggg ccagagcacg aaagttaata ccacgtttgc
     1921 acagctcctc caacagcacg acaagatgcc acatactgcg ccccagtcgg tgcggtttac
     1981 agactacccg tgcgtccacc gccgataatg tcctgagcag ttttttcagt ccggacctcc
     2041 tgccacggtt ttacgttgaa gaggtaaccc tgagcacgca gttcttcagt caggcgtggt
     2101 gcaccgtaac gctgttattg ctgggtaaga tcaaaaaact ttcaggcagc taaggaaagt
     2161 tgaaccagac attagatgaa atatttcaac caattacagc accaatttaa actcagtaaa
     2221 tcacagcaca gatcaagggg ttacgtgaaa gcgtagcccc tttttctttg gtagtggcag
     2281 caaaatggtt gtagtgtaaa aaataatccc gtttaatcaa tcaataatac atattgtttc
     2341 aatctacgtt attatctctt tgtaaaaata gccatttatt aatcattgaa aactgctttt
     2401 agaacttgat acaacgggac tagtcacaac aggactattc tcaacgggat catcctcaga
     2461 ggaactatca tcaaagtcat catccataaa taaaatatca tcgaatggtg ccacgcccgt
     2521 gatgagtttt attttattat tacgatcagt caagactcca cttaaaccgt tttcgctcac
     2581 aggttttaat gatttttcat tactcttgtt gtaagcaggc gcattaaaaa tacacggagt
     2641 atcaagatca aacaatgacg ttccccagtt cacatattga atatcatagt tactgaagtt
     2701 ctgtccagaa aagaagcatc ccttaaaatc ca
//
LOCUS       DAZRUF010000339         2726 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZRUF010000339 DAZRUF010000000
VERSION     DAZRUF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.342"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(419..676)
                     /locus_tag="ACHXOL_004093"
     CDS             complement(419..676)
                     /locus_tag="ACHXOL_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810915.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(673..1170)
                     /locus_tag="ACHXOL_004094"
     CDS             complement(673..1170)
                     /locus_tag="ACHXOL_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR6810916.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1372..1830)
                     /locus_tag="ACHXOL_004095"
     CDS             complement(1372..1830)
                     /locus_tag="ACHXOL_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR6810917.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1827..2324)
                     /locus_tag="ACHXOL_004096"
     CDS             complement(1827..2324)
                     /locus_tag="ACHXOL_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR6810918.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2324..2539)
                     /gene="essD"
                     /locus_tag="ACHXOL_004097"
     CDS             complement(2324..2539)
                     /gene="essD"
                     /locus_tag="ACHXOL_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR6810919.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          647 a          707 c          621 g          751 t
ORIGIN      
        1 agcgcatgat gtgtcaggct tgaatacctt tatcctttaa aagggatatc agttaagtta
       61 tcccgtgtag ggtataagcc attatcaaag ccactctgta gggaatggct tttgtgatgg
      121 catcacttac tctttacgct gctatcccac tcatcccgga attttgatgg gttattgaaa
      181 ccttctgctg acataacaac tccttcaatg tttggctgaa attgggatgt ctttccatca
      241 gtccgccacc acaaagaatc ttttttgcca taaggcagga ggttcatctt tcagtggctg
      301 ccggtgttat ttccccactt actggcttgg gttgtttcgt ggtactgccg ttaactggtg
      361 gcccagaata aattccggtt tcattatcaa gcccacccgt aaatgggctt tgtaatggct
      421 acttcgcttt tgcttccgct cgcttacgcc ggcgctcttc tttcctctcg gcttttgcca
      481 tgtccatgaa tgcctgcatg atcgagttcc gcatcatgta gctaacaaag tgatgattga
      541 cacagccgtt gaggcgcagc tgctcgccaa actcatccac cgaggccaat gcttccatca
      601 tgcccttctc gcctttcatg aactctgaga agtcgcgccc cgctctggag gcgcattcaa
      661 taacatgatc actcatcccg gaagcccggg gatcgtaatc tgcagctggt tagccaggga
      721 gttaatctca gcgaccaaca ctggcttcgt atagcgccat gctgccagcc cttgtccgca
      781 gaagctcgcc atgtctttct tctggtcaaa ttcatgacat ttcatgttga gctgcgcact
      841 taagctgttg cgatgctgaa gttctccggt gaagtagtca tcgaggactt tataggccgc
      901 gtacttgaac ccggggttta accaagccgc ataatcgtaa gcaacaaact tcccgccata
      961 tgttccaccg tgtacaccgc gctcagtaaa aaccacagat tcgtggtttt tctccagctc
     1021 ggctaagaac tctttggtct gcttgtttcg caggtagtgg taaggcgatt cagattcact
     1081 tttaccactg gctttccaca tatcagtgag gcagatcatg ccatcttcac cgatacgaat
     1141 tggttgattg aagagggtta atgatttcat agcgtgtacc tactctttga aatgaacctt
     1201 tgccgcacag gaaaccagcc caccgaggct cgccagcact aactggtatc ctcaaaggcc
     1261 cattccaaag gggcaggttc ggtgtaaaaa acatgcgttg cggtacgcat ttattgcaaa
     1321 aagccccgca tcgcgaggct cattaaatgg actttgtgat ttgcaaaaaa attatttcag
     1381 gcattgcgtc ctgatgtatt cctgcaggta gttaacctgc gcggttatct tgtcgattcc
     1441 acttcggaga cggtaataat tgagttcagc atctgctgta agtcctgggc tttctccatc
     1501 gcccatgccg ctggctccgg tcgttgactt tgcacaggtg gcggcgactt gcaggcgctt
     1561 acgcccagca gaaacatcag cacggagact ttcgatagtc gcgttagcat cagcaagctc
     1621 ctttatgtat cttgcgtcga gttctgctac atcacgttga cgcttccgca tgtcagcgat
     1681 ggtggcgttc gccttctcca gttcactggc cttgttatcg cgctgctctt tgtaggcgat
     1741 tgcgttatca cggtaatgat taacagccaa tgacaggcag acgatgatgc agataaccag
     1801 agcggagata atcgcggtta ctctgctcat tgtttccccc acaaacagac ttcacgctca
     1861 atctcacggc gagtcatcag tcctttccat tgattaccgc cagcgtatgt ccagcgccgt
     1921 agctgatcac atgcgccttt aatatcaccc tggtttattt tgcgaagaag cgtcgatgtt
     1981 ctgaaattgc cagcacccac gttgtagacg aacgagtaaa gagcgcagcg cgttgtttcc
     2041 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     2101 aggagggctt tgcactctgc ttcggtatac gttttaccgg gaatgatgtc ttttcctgta
     2161 tgcccgtaac atacagtcca tacaccaact atgtctttgt aaggattatg tctcacacct
     2221 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aatagccccg
     2281 ccaccaatag cagcagcaac agcttttcgt aatgatggag gcattatcca cctctcgcag
     2341 ccttgcgctt atcttcttta atcttgaaat aaaggtttgt caggtacgtc agcaggccaa
     2401 ataccaggct acccagcact ccaattgccg cccactgtga gggcgtgact ttatctagca
     2461 gctgtaaaaa ccagtacccg gcactacctg ctgaggtgcc ataggcgaca cccgttgtta
     2521 acttatccat ggatttcata accccacctc gcagacaaag cgggtgtaaa ttgagggaat
     2581 actacgaaac gtaacagact cggagtcagt gaataactca ggtattgggt tatcagctaa
     2641 tatcgagact caaaaaatgg aaaaacccgc tcgacggcgg gtttaagctg tgtgacgaag
     2701 taaccactct taacagcata accaat
//
LOCUS       DAZRUF010000340         2706 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZRUF010000340 DAZRUF010000000
VERSION     DAZRUF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.343"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..827
                     /locus_tag="ACHXOL_004098"
                     /pseudo
     CDS             1..827
                     /locus_tag="ACHXOL_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            827..1117
                     /locus_tag="ACHXOL_004099"
     CDS             827..1117
                     /locus_tag="ACHXOL_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810920.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            1438..1728
                     /locus_tag="ACHXOL_004100"
     CDS             1438..1728
                     /locus_tag="ACHXOL_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810921.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHTALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            1728..2147
                     /locus_tag="ACHXOL_004101"
     CDS             1728..2147
                     /locus_tag="ACHXOL_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810922.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYVKSLINKDN"
     gene            2315..2638
                     /locus_tag="ACHXOL_004102"
     CDS             2315..2638
                     /locus_tag="ACHXOL_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810923.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNAENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
BASE COUNT          922 a          489 c          566 g          729 t
ORIGIN      
        1 gaagaaatta acgcagcagc aagattgatt tatatggtca tggtccatag ctgtgacttc
       61 tatgaagaga aatacaaatc atattcacca agtattgacc atctcgctta tatggctggt
      121 gtatcaaaga gaacagcaat cagagccatt gagacacttg agaaacacgg tttaattcag
      181 gtgcaaaaga atcaggggcg tcaaagcact tatcgtgtat tcacactgga tgataagccg
      241 gaattacttg taagacctac atcacaggaa gttgcagcct cagtaagaca aaatgacacc
      301 actaccagtg acaaaatgtc atgggggtag tgacattttg tcacccgtgt tagagagata
      361 agttattaga taaaacatta gataataata ttacagataa gaataaagag actggactta
      421 atgatgatat taaagaagat gactttcttt ctttggatga attaaatgat tttctctcta
      481 tgaatgaatc tgaccttgaa gtaagctcta aaaagacttc taaacaagac agagaaatca
      541 ctgatgtgag tgttgatagt gaccataaaa acacacagtg ccccgccact gatgatgagt
      601 tggacccctt cgatgatgaa gaggatgaac gtgaaaaagc cattaagagg gcagagaaga
      661 aaaagagatt cgagcttgca agacaaaaga acaaagtatc gggtttagac ccattcgatg
      721 acagtgaaga tgattattat ctttcaccga aaaagaaaca aactcaggat gacaagaagc
      781 aaaaaattaa accgaagatg tattttgccg gtggaggaat taagtaatga aagaatatca
      841 cggtgaaaag agatacaaag attacctgtt aaagcgttac agcatttctc gtgaaggtca
      901 tcttatgaag gatactcacg gggaagttta ccggattaga ccaaagaaag aagggagggg
      961 ttatttcttt tacgatggtg tgacagattt aaagattgac gctttgaggt ttgcagtgct
     1021 gtatcatttt gatatctgga attcaatcca ccagctacga ttaaaagacg gtgaccccag
     1081 caacttaaag gccaccaata ttatcacaaa acgttagttt gttttaccat catcaatcgt
     1141 aattgtgatt tcatatttga aaagcttaat gctgaaattg aatcgtgtac ttctggcgat
     1201 aatcattttt aaaagctgat acacaagaag caccagaaac acaaagcaac ctaagcttgc
     1261 gaatctgtga gattcagcaa ttgtttcaag aaagcccatt aattcccctt atttatcaaa
     1321 gggaatgacc tgtatcagcc ccctacaacc atattaacat gtaaatgagt tgttgacaat
     1381 atgttataaa tatgttaaag ttagttataa gtcaaagatt ttaagaagga aaatttaatg
     1441 agacacacag ttacacgcct tttgccagag gcatattcca gactggcagc aatcaaagac
     1501 aaatacccag ccatgagcca cacagcactt gttggtgaga ttctgaagga acacacagcc
     1561 cttacaccgt ttgacgctat caagggcgtt gcagagggtt ctaaggccat cagaatcaca
     1621 acgggccaac tggaactact caaagcacag tccgcagtgg caggttgctc aatgattcag
     1681 gtgttgagtg ccaaggttca tcaactggcg gataagctgg gggtttaatg ctatgcacat
     1741 ttgattatca tgcgttttat cgccatagag gtcaccttgt ccgtgagaat aacggaacat
     1801 ggaaagattt aactcgtaat gctgaaccct tcacacacca gcaattaaaa tcccgcttct
     1861 ccagatggaa gaatgcacca gcagatactg gtgattttga gattatccat accttcgatt
     1921 atgcctcaga agatgacctt cacgatgcaa ttaagctgat tgaagacttc aatacctcag
     1981 aagatgtgga tggtgacaaa tataaatccc gtggtaaccg ctataaccgt gatgatatca
     2041 ctcaggccat aaatgctggt caatctcagc gatacattac agaaaaatat ggtatttcac
     2101 ggagacagta tttctacgtt aagtcattga ttaataaaga caattaaaaa taaccatcta
     2161 agaaacggta gaatgcactt tacaggcaga gttgcacctt aatttgataa attaccttac
     2221 cagccagtgc aactccctct gtaacccgca tggttactgg cctcagaatg gctattttaa
     2281 gaggtctgag gtgtgtttca taggagattt aaaaatgaat aattacccaa tgcaaattat
     2341 ggttgataac gacactgcta tgatggtgca aagcttcatt gatgcaggta tcgcaattaa
     2401 ttttgataaa ttactaaagc tgatggctga taatgcagaa aacatctctg actttattca
     2461 gtcggtggag tttaacgagc ctcgaatgat gctaccaatt acagacagca acatgaaaag
     2521 attggttatc gagcaaacga acatatacag tatttcccca gagcaatttt tgaaaggtgc
     2581 tgtgacaatc ctgtacagtg ataatattct ggtgacggat tctgtgaggg ttcactaatg
     2641 ggtttaataa ttgcttggac gttgcttggc ttatttgttg gttgcattat taacaatgcg
     2701 gtaaat
//
LOCUS       DAZRUF010000341         2702 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZRUF010000341 DAZRUF010000000
VERSION     DAZRUF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.344"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..407
                     /locus_tag="ACHXOL_004103"
     CDS             <1..407
                     /locus_tag="ACHXOL_004103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810924.1"
                     /translation="KKVRGDIELKYFCKMVNKRGLSPEEYFSLDPDVLNMLMIYDTFI
                     EPSGTQIEMMKHAYQCYYTTISNGNLTPEARKSIKVQDFDFLDVLSDSTKSTSEKAEE
                     RKLKTKEAQSNDIKSIGELIKSQVLGKKKNGK"
     gene            397..2610
                     /locus_tag="ACHXOL_004104"
     CDS             397..2610
                     /locus_tag="ACHXOL_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810925.1"
                     /translation="MANNNRIRVDITGDSSGLQRALRSGTESLEEFGSTAGGIVEEFT
                     GRITGMAGGFGTAMTGIAGAAAIGIGGLAALVESSREYVREMNQISKSTGLSVVQLQQ
                     LSAAFSGLGLEIDKFGDFNKDTLDKLGDAFRAGGGVSDDLKEYGLNLQDYNKYLKQAD
                     GGMQAVIHTFYAMRDAGKSQSEIVNVMETLASDSSHMISTLQQFNNEAQATAYIQSQN
                     AEVTNDAAEKYAEFDKNLGKLTTSIKGTIADGLSPLVNAMNSVYDAANQKPHEAGLFE
                     DLNERIKTSKGSLQDMLDIWEKLRTAGALNYQGAALNTGIMDDKGNKGNEFAQAKARL
                     EELANNLKSDVAVATAPTGGWVDQAKEAEDAAKKLEQQRKQAEAAQKAADAKRLQAQR
                     NLEAALAQVGEDGFQVRLQQFDRQQKALLKTITDSAQVLGINPDEMLKNATAYGAKQR
                     TDLLNSMVGYQDPNQGLKDTNALIGSGLLNDNQKGYLAQQQNQRINGGNPLAYNDTDQ
                     KLKDNADAMNAELQQNELLLKGHEDYEKRKAEITAKYNAQAIQISNQNAQDQLSIFSS
                     TAQSLSQGMVDAFGESSGAAQAAFTLSKSISIAQTVLSIQSALAQALATPWPASLANY
                     AQVLSLGMSIISTAKGAAAGQFHGGVDELPASYDNKSFVLKQGERVVQPEANKKLTAF
                     LDKQEGGSTSGDITVNAPLIIQGDVADDDKKFNEMLKKHANSVSQAVRSSQKRNT"
BASE COUNT          875 a          494 c          646 g          687 t
ORIGIN      
        1 gaaaaaaagt aagaggtgat atcgaattaa agtacttttg taagatggtt aacaaaagag
       61 gcttatcacc tgaagaatac tttagtttag atcctgatgt actcaatatg cttatgatct
      121 acgatacttt tatagaacca tccggtacgc agattgaaat gatgaaacat gcgtaccagt
      181 gttactacac cactatcagt aatggcaacc taacaccaga agcaaggaaa agtatcaagg
      241 tacaggattt tgattttctt gatgtgctta gtgatagtac taagagtact tcagagaaag
      301 ctgaagaaag aaaattaaaa actaaagagg ctcaatccaa tgatattaaa tctatagggg
      361 agttaattaa atctcaggta ttaggaaaga aaaagaatgg caaataataa tagaatacgt
      421 gtagatatta cgggtgattc ttccggttta cagcgtgctt taaggtctgg tactgaatca
      481 ttagaggaat ttggtagtac tgctggtggc attgttgaag agttcacagg ccgaataact
      541 ggtatggctg gcgggttcgg tactgctatg accgggatcg cgggtgctgc ggctattggt
      601 attggtggcc tggcagcatt agtagaatca tcacgtgaat atgtgcgtga aatgaaccag
      661 atcagtaaga gtaccggatt gtccgtggtt cagttacaac agctatcagc ggcgtttagt
      721 ggcctcggtc ttgagattga taagtttggt gatttcaaca aggatacctt ggataagctc
      781 ggtgatgctt ttcgtgctgg cggtggtgta tccgatgatc ttaaagaata tggactcaac
      841 ctacaggact ataacaagta cttaaaacag gctgatggtg gtatgcaggc tgtgatccac
      901 accttttacg ccatgcgtga tgcaggtaaa tcacagagtg aaatagttaa cgtaatggaa
      961 acgttagcca gtgactcaag ccacatgatt agtactcttc agcagttcaa taatgaggct
     1021 caagctacag cctatatcca gtcacaaaat gctgaagtaa ctaatgatgc tgctgaaaaa
     1081 tatgctgaat tcgataaaaa ccttgggaaa cttactacca gcattaaagg tactattgct
     1141 gatggcctat cacctttagt taatgccatg aacagtgtct atgatgctgc aaatcaaaaa
     1201 cctcacgagg caggtttgtt tgaagatctg aatgaacgta ttaaaacttc caagggttct
     1261 ctacaggata tgttggatat ctgggaaaaa ttacgtacag caggggcatt aaactaccag
     1321 ggtgcagcac tcaatactgg catcatggat gataagggca acaagggtaa tgagttcgct
     1381 caggctaagg cacgtttaga agaactggca aacaacctaa agtccgatgt tgcggttgct
     1441 accgctccta ctggcggatg ggttgatcag gcaaaagaag ctgaagatgc agcgaagaag
     1501 ttagaacaac aacgtaaaca ggcagaagcg gcacaaaagg cggcagatgc aaaacgccta
     1561 caggctcaac gtaatctgga agctgctctt gctcaagttg gggaagatgg ttttcaggta
     1621 cgtctacagc agttcgacag acaacaaaag gcacttctta aaaccatcac cgatagtgct
     1681 caagtactcg gtatcaatcc tgatgaaatg ctaaagaacg ctactgcata tggtgctaaa
     1741 caacgtactg atttactgaa ttcaatggtt ggttatcaag atcctaatca gggtctgaaa
     1801 gataccaatg ctcttattgg ttccggtcta ctcaatgaca atcagaaggg gtatctggca
     1861 cagcaacaga atcagcgtat caatggtggt aatccattag cctataacga taccgatcag
     1921 aagttgaaag ataacgctga tgctatgaat gctgaacttc agcagaatga gttgttgctg
     1981 aaaggccatg aggactatga gaaacgtaaa gctgagatca cggcaaagta caatgcacag
     2041 gccatccaaa tcagtaacca gaatgcacag gatcagttaa gtatcttctc aagtactgca
     2101 cagtcactat cacagggcat ggttgatgcg tttggggaaa gtagtggggc tgcacaagct
     2161 gcatttaccc taagtaagag cataagcatt gcacagacag tactttcaat tcagtcagca
     2221 ttggcccaag cactcgctac cccttggcca gcttcattgg caaactatgc acaagtactt
     2281 tcacttggta tgtcgattat tagtactgca aagggtgctg ctgctggtca gttccatggc
     2341 ggggtagatg aactaccagc aagttatgac aacaaatcat ttgtacttaa gcagggcgag
     2401 agagtggtgc agcctgaagc gaataagaaa ttaacagcat tcttggataa acaagaaggt
     2461 ggaagtactt caggtgatat taccgttaac gcaccattga tcatccaggg tgatgtagct
     2521 gatgatgaca agaaatttaa tgagatgctt aagaaacacg ccaatagtgt ttctcaagct
     2581 gtgagaagta gtcaaaaacg taatacataa gaaagcccag ttagggattt tccgtgtgat
     2641 aagacaaata ttgtgaaaga gaagatgaaa gggtatagag attgtaatac tcttcccgca
     2701 at
//
LOCUS       DAZRUF010000342         2682 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZRUF010000342 DAZRUF010000000
VERSION     DAZRUF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.345"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            231..2510
                     /gene="ydeP"
                     /locus_tag="ACHXOL_004105"
     CDS             231..2510
                     /gene="ydeP"
                     /locus_tag="ACHXOL_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR6810926.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT          713 a          622 c          658 g          689 t
ORIGIN      
        1 ttgaagccag agtcttattt tttatattga aaaatatgtt aatttaaaca ctatgagaga
       61 tgatgcttaa cttatcgtat ttaatctatt gtttaacgat aatagattaa aaatagaaag
      121 agagtagtcc cctactctta tatatccatg ttggcgataa tccctttgta tgtactgtgc
      181 atcatcgcta ttacaaatcc taataattca tttccacaca ggataagtag atgaagaaaa
      241 aaattgaatc ctaccagggt gctgcaggtg gttggggtgc tgttaaatcc gtagcgaatg
      301 cagtacgtaa gcagatggat atacgccatg atgttattgc catgtttgac atgaataagc
      361 cagagggctt tgactgtccg ggttgtgcat ggccagatcc taagcacagt gcgtcattcg
      421 acatttgtga aaacggcgca aaagcaatcg cctgggaagt cacggataag caggtaaatg
      481 cctctttctt tgctgagaat acggttcaat cattacttac ctggggagat cacgagcttg
      541 aggctgcagg gcgtctcact cagcctttga aatatgatgc cgtcagcgac tgttacaagc
      601 cattaagctg gcaacaagct ttcgacgaaa ttggcgcacg ccttcaaagc tatagtgatc
      661 ccaatcaggt tgaattctat acttcgggcc gcacttccaa tgaagctgcc tttctttatc
      721 agctttttgc ccgtgaatac gggagcaata actttcccga ctgctccaac atgtgccatg
      781 aaccgacaag cgtgggtttg gcagcgagta tcggtgtagg taaagggacc gtgttgctgg
      841 aagactttga gaagtgcgat ttagtcattt gcattgggca taaccctggt acaaaccacc
      901 ctcgcatgct gacttcgttg cgcgctttag tgaaacgggg agcgaaaatg atcgccatca
      961 atcctctaca ggaacgtggc ctggagcgat ttaccgcacc gcaaaacccg tttgaaatgc
     1021 tgacgaactc tgagactcag ttggccagtg cctactacaa cgtgcgcatt ggtggcgata
     1081 tggcgttgct caaggggatg atgcgcctgt taattgagcg cgatgatgct gcaagtgccg
     1141 caggtcggcc ctcattgctt gatgacgaat ttattcaaac gcataccgtc ggctttgacg
     1201 agctacgccg tgacgttctc aattccgagt ggaaagatat cgaacgtatt tctggactaa
     1261 gtcagacaca aatcgccgaa ctggctgacg catatgccgc tgccgatcgc accattatct
     1321 gttacggaat ggggatcact cagcacgaac atggtaccca gaatgtacag caactggtca
     1381 atctgctgtt gatgaaaggt aacattggca agcctggtgc gggtatctgc ccactacgag
     1441 gtcactctaa tgtacagggc gaccgaaccg tcggtatcac cgagaaaccg tctgcagagt
     1501 ttctggctcg cctgggtgag cgctatggct tcaccccacc tcatgcacct ggacatgctg
     1561 caattgccag catgcaagca atatgcacgg ggcaggctcg agcattgatc tgcatggggg
     1621 gcaactttgc actggcaatg ccagatcggg aagcgagcgc tgtaccgtta acgcaattag
     1681 atttggcggt acacgtagcc actaagctta accgctctca tctgctgacc gcacggcata
     1741 gctatattct gccggttctg gggcgtagcg agattgacat gcaaaaaagc ggtgcgcagg
     1801 cggtaaccgt tgaggattca atgtcgatga ttcatgcctc gcgtggcgtg ttaaaacccg
     1861 ccggtgtaat gctgaaatca gagtgtgcag tggtcgcggg aatcgcgcag gcagcactac
     1921 cccacagcgt ggtagcctgg gagtatctgg tggaagatta tgatcgcatt cgcaatgaca
     1981 ttgaagctgt gttaccagag ttcgccgact ataaccagcg catccgacat cctggaggtt
     2041 ttcacctgat aaatgcagct gctgaaaggc gctggatgac gccgtcaggt aaggctaatt
     2101 tcattaccag caaagggctg ttagaagatc cctcttcagc gtttaacagt aagctggtca
     2161 tggcgacagt acgtagccac gatcagtaca acacgacgat ttatggtatg gatgatcgct
     2221 atcgaggggt attcggtcaa cgagatgtgg tctttatgag tgctaaacaa gctaaaattt
     2281 gccgtgtaaa aaacggcgaa agagttaatc ttattgcgct tacgccagac ggtaagcgca
     2341 gctcacgccg catggataga ttaaaagtgg tcatttaccc tatggctgac cgctcactgg
     2401 tgacctattt tccagaatcg aatcacatgc taactcttga taaccacgat ccattaagtg
     2461 gcattcctgg ttataaaagt attccggttg aattagaacc atcaaattaa ttcgcctcct
     2521 catttcttct gctgtcatcc gcacagcaga agaatttctc attgactatt atcgcgcaat
     2581 ttgctcacat tgattaaatt aaaccacaca ctataagata taaacctctg cctacagctg
     2641 taagaaactc cgctcagtac tgaagtgcca gtcctatttc ct
//
LOCUS       DAZRUF010000343         2670 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZRUF010000343 DAZRUF010000000
VERSION     DAZRUF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.346"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..656
                     /locus_tag="ACHXOL_004106"
     CDS             <1..656
                     /locus_tag="ACHXOL_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR6810927.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            614..1291
                     /gene="bglJ"
                     /locus_tag="ACHXOL_004107"
     CDS             614..1291
                     /gene="bglJ"
                     /locus_tag="ACHXOL_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR6810928.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHXOL_004108"
     CDS             complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHXOL_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR6810929.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2258..2494
                     /locus_tag="ACHXOL_004109"
     CDS             2258..2494
                     /locus_tag="ACHXOL_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR6810930.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2533..2619)
                     /locus_tag="ACHXOL_004110"
     tRNA            complement(2533..2619)
                     /locus_tag="ACHXOL_004110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2583..2585),aa:Leu,seq:cag)
BASE COUNT          684 a          597 c          684 g          705 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc aattcacagc aacgctgaaa
      541 tgtatgcatg gataaatagc gcgcagggtg caagagaact taacttgcct tctgtttatg
      601 gagatgccgc agaatggaac acagccgaat taagaagaga aatgtcgcac tcatagaaaa
      661 atgcgtcatg agcagtatcg gtattgagag tttattcaga aagtttgcgg gtaaccctta
      721 taagctccat acctatacca gtcaggagtc atttcaggat gccatgtcgc ggatctcatt
      781 tgcggcggtc attttttctt tttctgccat gagaagtgag cgcagagagg gattatcttg
      841 cctgactgaa ctggcgatta agtttccgcg tacccggcgt ttagttattg cggatgatga
      901 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
      961 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
     1021 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgc taagcccgac
     1081 ggagagagaa atattgcgct ttatgtcgcg tggctactca atgacacaaa ttgccgagca
     1141 gcttaaacgc aatatcaaaa cgatccgtgc acataaattt aatgtgatgt cgaaactggg
     1201 cgtcagttct gatgcagggt tgttggaggc cgcagatatt ctgttatgta tgcggcattg
     1261 cgaagcaagt aatgtgttgc atccctatta atccgcatga tgccgggttt acttccccgg
     1321 cagtgctttc atttcagcgt acaatcgcca cattgctgca catccggtaa gcgataacgc
     1381 tggcagcagg ttcggcgcac cagcaggcca tcgcgcagca cgacggtacg ccagagtgga
     1441 ttatcttcac cgttcgtgag cgttttctca aaaaagaggg catggcgcag cgattcaacc
     1501 gtcgcctcgc cgagcagttg cttcatctca gtgagatacc agttgatcaa ataaccggta
     1561 ttactccaga taagtttgcc gttgatctct ccggtcgctt ctagtgcctg cacaaccgga
     1621 accagcgcct ggctgattaa cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt
     1681 ttatcttcac acacatcgac ccagaaacag gcgacgcgtc cggtttcgtg aaactcagca
     1741 tggaaatgtt ccggcgacac atctaatgcc ttttcctgcg tcagtagcgc cagcattaat
     1801 ggtggcacca tcaggccgat atacaattgt gcccatagtg agatcagcgg tttgttctcg
     1861 cggatcatcg ttggttggtt gcgatagata tgatcggaat agaccgcgag tagagaactt
     1921 agcgcattcg gtgatgacca ttgcgccagc gtcatggcgt taagcggagc gggttcatcc
     1981 aggcggataa actcgagcaa atgttcacga tgtttggcga tcgtcgcacg tacggcttgt
     2041 gcaagcccag catcctgcgg ctggagatgc gttcgccaga tgatatcttc atagagcggt
     2101 gcggaacgat aggccataat cgggatagta atctaaatga taatgattgc taatcctagc
     2161 gataggttta cccgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     2221 gatattggtt atcattatca atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag
     2281 tggctgggga gtgttgctgc cgggattgct gattgcaggg ctgatgtatg cggatttatc
     2341 gccggatcag tggcggattg tcattctgat gggattagta ttgacgccgg tgatgctgta
     2401 tcacaaacag ttacggcatt acgttttgct accatcgtgc ctggcgctta ttgctgccat
     2461 catgctgatg ataatgaatt tgaatcaggg atgaaaaatc aaggaagaaa caagaaagga
     2521 agtaaagata attggtgcga ggggggggac ttgaaccccc acgtccgtaa ggacactaac
     2581 acctgaagct agcgcgtcta ccaattccgc caccttcgca cagtcatctt actttttttg
     2641 atatcgcctc gtttggtgcg aggggggaga
//
LOCUS       DAZRUF010000344         2624 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZRUF010000344 DAZRUF010000000
VERSION     DAZRUF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.347"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..181)
                     /locus_tag="ACHXOL_004111"
     CDS             complement(<1..181)
                     /locus_tag="ACHXOL_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR6810931.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="ACHXOL_004112"
     CDS             425..682
                     /locus_tag="ACHXOL_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR6810932.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHXOL_004113"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHXOL_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR6810933.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHXOL_004114"
     CDS             complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHXOL_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR6810934.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT          677 a          612 c          624 g          711 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat ttta
//
LOCUS       DAZRUF010000345         2611 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZRUF010000345 DAZRUF010000000
VERSION     DAZRUF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.348"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            96..605
                     /gene="eco"
                     /locus_tag="ACHXOL_004115"
     CDS             96..605
                     /gene="eco"
                     /locus_tag="ACHXOL_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR6810935.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(914..2560)
                     /gene="mqo"
                     /locus_tag="ACHXOL_004116"
     CDS             complement(914..2560)
                     /gene="mqo"
                     /locus_tag="ACHXOL_004116"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR6810936.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          614 a          724 c          653 g          620 t
ORIGIN      
        1 aattgtctga atgttcttta agttattcat aaagcaaatt aataaatctg atgaatatgt
       61 taaccttcag cgacatcatc ggtgaaaacc tataaatgaa gaaggaaagc aaaaaaatga
      121 agaccattct acctgcagta ttgtttgccg ctttcgccac cacttccgcc tgggcggcag
      181 aaagcgtcca gccactggaa aaaatcgcgc cttatccaca agctgaaaaa gggatgaagc
      241 gtcaggtgat tcagttaacc ccgcaagaag atgaatctac cctgaaagta gaactgttaa
      301 ccggtcagac gctggaagtc gattgcaatt tgcatcgtct cggcgggaag ctggaaaaca
      361 aaacgctgga aggctggggc tatgattatt atgtctttga taaagttagc tcgccggttt
      421 ccacgatgat ggcctgcccg gatggcaaga aagagaagaa atttgtcacc gcgtatctgg
      481 gcgatgctgg aatgctgcgt tacaacagca agctgccgat cgtggtgtat acgccagaca
      541 atgtagatgt gaagtaccgc gtctggaagg cggaagagaa aattgacaac gcggtagttc
      601 gctaaactgc tgtgaagtgc gtcaccccgt aggtcggata aggcggtcac gccgcatccg
      661 acatctaacg cccaagccgg ttgcctgatg cgacgctggc gcgtcttatc aggcctacac
      721 cgctgtgaag tgctccaccc cgtaggttgg ataaggcggt tacgccgcat ccgacatcta
      781 acgcccgagc cggttgcctg atgcgacgct gacgcgtctt atcaggccta caccgctgtg
      841 aagtgctcca ccccgtaggt cggataaggc ggttacgcca catccggcat accatgccgg
      901 atgtggcaca tcattacaac gcaatatccg ccacttcttt ttgaacgggt tgcggtttca
      961 actgcggttt cggcgtacta tctgctgcct gcggcttgtc gtatttcagg cccagcactt
     1021 cgctggtgta ctgcaactcg cgttctgttg ccgctacatc accgttcagc ttgcgtccat
     1081 aagatggaac gatcgctttc aacgtagcct gccattgcgg gctggaaacg cgatcgccaa
     1141 atactttttc cagcagatcc aacataatcg gcgcagcggt tgacgcccct ggcgatgccc
     1201 ccagcagcgc ggcaatggtc ccttgctggt cactgaccac ttcagtaccc agacgcagta
     1261 caccaccttt atcggcatca cgcttgataa tctgcacacg ctgccccgct tgccacaaac
     1321 gccagtcctc ttttttcgcc tgcggatagt actctttcaa cgcttcaaaa cgatcctctt
     1381 cactcaacat cacctgactc accaagtatt tcaccagatc gaaattatcc agcccgacgt
     1441 gcatcatcgg catcacgtta gaggtggtgg tggaactcat cagatcccac aatgaaccgt
     1501 ttttgaggaa tttggttgag aaggtggcaa atggcccaaa cagcactacg cgtttaccgt
     1561 ccagaacgcg ggtatcgata tgcggaaccg acatcggtgg tgcgccaacg gatgctttac
     1621 cgtaaacctt cgccagatgg tgattaacca cgtccgggtt ttccgaaaca aggaactgtc
     1681 cgcccaccgg gaaaccggcg tagtctttcg cttccggaat ccccgattcc tgtaacagct
     1741 tcagcgccgc accgcccgcg ccgataaaga caaatttggc acgaatgttc tgtgcagtgc
     1801 catttttcag atcggcaacg gtaacggtcc aggtgttatc gtcattacgc tttagggcgc
     1861 ggacttcgct gctgagttgc agcgagaagt tagatttctt ctgcaaggaa gcaattaact
     1921 ggcgggtgat ctcgccgtag ttcacatcgg taccaatttc cgtacgcgtg gctgccactt
     1981 tctgttgcgg atcgcgccct tccatcacta acggtgccca ctctttgatc tgcgcgtgat
     2041 cttcagagta acgcataccg cgaaacagcg agctttgttg caacgcggcg taacgggcgc
     2101 gcaggaaatt aacgttatcc tcgccccaga caaagctcat atgcggaacg gtattgataa
     2161 atgaacgcgg agtacgcagc acgccgcgct cgacctggtg cgcccagaac tggcgggaaa
     2221 tctgaaatgc ttcgttaatg gcgactgctt tttcaatact gatactgcca tcggcgtttt
     2281 gcggggtgta gttcagttcc atcagtgcag aatgcccggt tccggcgttg ttccagccgt
     2341 tcgaactctc ctgcgcgaca ccctccaggc gctccaccat ggtcatcgac cattcaggct
     2401 ccagctcgcg taaataggtc cccaacgtgg cgctcataat gccgccgcca atcaacagta
     2461 catcagtttc ctgctcctcg gacgctttcg cttttgccgc catcgaaacg gcattaagcc
     2521 ccacggccat cgagaagagc atggcagtca cttttttcat cttgttaatg ccttactttt
     2581 agtcgcttta ttgcaggtga gatttgcgcg g
//
LOCUS       DAZRUF010000346         2609 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZRUF010000346 DAZRUF010000000
VERSION     DAZRUF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.349"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            93..1706
                     /gene="mppA"
                     /locus_tag="ACHXOL_004117"
     CDS             93..1706
                     /gene="mppA"
                     /locus_tag="ACHXOL_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR6810937.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1757..>2609)
                     /gene="ynaI"
                     /locus_tag="ACHXOL_004118"
     CDS             complement(1757..>2609)
                     /gene="ynaI"
                     /locus_tag="ACHXOL_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR6810938.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT          747 a          587 c          630 g          645 t
ORIGIN      
        1 ctgtgtcact tttttgatgg aagttgtttg catttcttta aggcgaaaca aataattacg
       61 catcaatttt aatgtcggtt agagggaaac ttatgaagca ctctgtttca gtcacgtgtt
      121 gtgcgctgtt ggtcagcagc atttctcttt cgtatgctgc agaagttccg agcggcacag
      181 tactggcaga gaagcaggag ctggtgcgcc acattaaaga tgagcctgcg tcgctggatc
      241 ccgctaaagc cgtgggcctg ccagagattc aggtcattcg cgatctgttt gaaggactgg
      301 tgaatcagaa cgagaaaggg gagattgtcc ccggcgttgc gactcagtgg aaaagtaatg
      361 acaaccgtat ctggactttt accctgcgcg ataacgcaaa atgggcggat ggcacaccgg
      421 taacggcgca agattttgtc tacagctggc agcgtctggt ggacccgaaa acgctatcgc
      481 catttgcatg gtttgccgcg ctggcgggaa tcaacaacgc acaggcgatt attgatggta
      541 aagctacgcc agaccagctt ggcgtcaccg cagttgatgc ccatactttg aaaattcagc
      601 ttgataaacc gttgccgtgg tttgtgaatt taaccgctaa ctttgccttc ttcccggtgc
      661 aaaaagccaa cgtagaaagc ggtaaagagt ggacgaaacc cggaaatctg atcggcaatg
      721 gcgcttatgt tcttaaagat cgcgtagtca atgaaaaact ggtcgtggta ccgaataccc
      781 attattggga taacgccaaa acggtactgc aaaaagtgac cttcctgcca attaatcagg
      841 aatccgcagc cactaagcgt tacctcgcgg gggatattga tatcaccgaa tccttcccga
      901 aaaatatgta tcagaagctg ttgaaggata ttccggggca ggtttatacg ccgccgcagc
      961 tcgggaccta ttattatgcg tttaacacgc aaaaagggcc gacggcagat cagcgcgttc
     1021 gtctggcatt aagtatgacg atagatcgcc gcctgatgac cgaaaaagta ttagggacgg
     1081 gcgaaaagcc agcgtggcat tttacaccag atgttaccgc cggatttacg ccggaacctt
     1141 cgccgtttga acaaatgagt caggaagaac tgaatgcgca ggcaaaaact ttgttgagcg
     1201 cagctggtta tggtccgcaa aaaccgctga agctgacgct tttgtataac acttcagaaa
     1261 accatcaaaa aattgcgatt gctgtagcat cgatgtggaa aaagaacctt ggcgtagatg
     1321 ttaaattgca aaatcaggaa tggaaaacct atatcgatag ccgtaacacc ggcaattttg
     1381 atgttatccg cgcctcatgg gtgggggatt ataatgaacc ctccactttc ctgacattat
     1441 taacgtcaac gcattcagga aatatttcac gctttaacaa tccggcatac gacaaagttc
     1501 tggcccaggc atcgacggaa aataccgtta aagcgcgtaa tgccgattac aacgcggcag
     1561 aaaaaatcct catggagcaa gcaccgattg caccaattta tcaatatacc aatggacgat
     1621 taatcaagcc gtggctgaaa ggttatccca ttaataaccc ggaagatgtg gcgtacagcc
     1681 ggactatgta tattgtgaag cattgatgtg atgggaactg gcgttaccct tgtggataac
     1741 gccagtgatg tctgatttag cgcccctgtt ccggcggtgt aatgttatcc atatacagcg
     1801 tctggctggg gaaggcaaag tccgcgccgt gtgactgcac aatatcgata atcttcaaat
     1861 aaacgtcttg ctgtgcagca agccattcag cccataccgt ggttttggta aagcaataaa
     1921 ccataatatt caatgacgag tcggcaaact ggttgaaata aaccagtaag gtttgtcgct
     1981 ggtcgatggc cgggtgattt ttcagcatct cacgtacggc ttcgacaata acgcccactt
     2041 ttgccgcatc ctcataacgt aaaccaatgg tcgtggtaat gcggcggttg gtcattcgtc
     2101 ctgggttttc tacgctgatc gacgaaaaca gcgagttcgg tacgtacaat ggacgattat
     2161 caaaggtcgt aattttggta attcgccagc caatttccgc tactgtacct tcgatatttc
     2221 tgtccggtga acggatccag tcgccaatac tgaaaggacg gtcgaaatag agcatgatcc
     2281 cggaaaagaa attactcaga atatctttac cggccatacc gacagccaga ccaccaatac
     2341 caccaaaggt cagcaagcca gaaaggctca tgccgaaatg ttcgccataa agcagaacaa
     2401 gcaccacaat aatggtgatt ttgataatgc gcgacataat ccgcgcactg gtgatatcgc
     2461 gacctttttt aatctgctgt ttttcaaact gattaatcag caaaaatagc ttaatcgtca
     2521 gaataaccgc aatcagggac gtacagataa aatcgataac gtctggggtg ataaatttga
     2581 gtttatagtt ttctataaca taattaata
//
LOCUS       DAZRUF010000347         2604 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZRUF010000347 DAZRUF010000000
VERSION     DAZRUF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.350"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            9..512
                     /locus_tag="ACHXOL_004119"
     CDS             9..512
                     /locus_tag="ACHXOL_004119"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR6810939.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(558..974)
                     /gene="rraB"
                     /locus_tag="ACHXOL_004120"
     CDS             complement(558..974)
                     /gene="rraB"
                     /locus_tag="ACHXOL_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR6810940.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1136..2140
                     /gene="argF"
                     /locus_tag="ACHXOL_004121"
     CDS             1136..2140
                     /gene="argF"
                     /locus_tag="ACHXOL_004121"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR6810941.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2212..2535)
                     /locus_tag="ACHXOL_004122"
     CDS             complement(2212..2535)
                     /locus_tag="ACHXOL_004122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810942.1"
                     /translation="MKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNK
                     LPGYVVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKK
                     LGWNN"
BASE COUNT          691 a          618 c          591 g          704 t
ORIGIN      
        1 gttaaactat gaataacatt gcgccgcaat cacctgtaat gcgtcgcctg acgctgctgg
       61 ataatcctgc tatcgcccgc gtcattcgtc aggtatccgc cgaatacggt cttaccgctg
      121 ataaaggcta caccgtcgcc gatccgaatc ttgacgagct gtatcaagtg tatagtcagc
      181 ctggccatgc atattgggtg gttgagtacg agggtgaagt ggtcggcggc ggtgggattg
      241 cgccattaac cgggagtgag tcggacattt gcgaactgca aaagatgtat tttcttccgg
      301 ctattcgcgg taaagggcta gcgaaaaaac tggccttaat ggcgatggag caggcacgag
      361 agatgggctt caaacgctgc tatctggaaa cgaccgcttt tttaaaggaa gccattgcgc
      421 tttatgagca tttgggcttt gagcatatcg actatgcgct tggctgcacg ggccatgtcg
      481 attgtgaagt gcggatgcta cgcgaactct aaaaaaatgc ccgctagtat gtgctatcgg
      541 gcattttaat caattaatta gtggcgaacc ccgtcatcgt cttcatcgac aaaatcttca
      601 tcgtcgccat cttcgccgtt aggatcttca aagtaagtgc cccaaccgtc gtactcaacg
      661 tcaaatttct ccgccagcgt catcagttgt tcaacctggg catcgatcag atcggcattc
      721 aacgcgcact cgctgaggat gtcgcagcaa atcacgatat caccatcttc aacttccagc
      781 tcttctggat cggtcacttc gtaaccgagt ttaaacgctt caactgccgc tttttccaga
      841 gtttccagat cgtctgcgga aagatggtgt tcgatggtgt acaacgcgtc cggatcgctg
      901 ccatcttcca gtaattcttc aataatcaaa cgtgtttctt cacgctgttc ttccagttgt
      961 tccgggtttg ccatggctca ttcctcttaa tgtcctgccg atactcttat tgtcacacac
     1021 cactgacatt gcctccacct ttgtgataaa gatttatgct ttagacttgc aaatgaataa
     1081 tcatccatat aaattgaatt ttaattcatt aaggcgttag ccacaggagg gacctatgtc
     1141 cgggttttat cataagcatt tcctgaaatt actcgatttc acgccagctg aactcaacag
     1201 cctgctgcag ttagccgcga agctgaaagc cgataagaaa agcggtaaag aagaagccaa
     1261 actcactggt aaaaacatcg cgctcatctt cgaaaaagac tcgacccgta cccgatgctc
     1321 tttcgaagtt gccgcatatg accagggggc tcgtgttact tatctcggcc caagcggcag
     1381 ccaaattggt cataaagagt cgattaaaga cactgcccgc gtgcttggtc gcatgtatga
     1441 cggtattcag tatcgcggct atggtcagga gattgtcgaa acactggcgg aatacgctgg
     1501 cgtgccggta tggaacggcc tgaccaatga gtttcacccc acgcagctgc tggcggatct
     1561 cctcaccatg caggagcatt tgcccggcaa aacgttcaac gaaatgacgc tagtctatgc
     1621 aggtgacgca cgtaacaaca tgggcaattc gatgctcgaa gctgcagcgc ttaccggtct
     1681 ggatttgcgt ctggtcgcgc cacaggcatg ctggccggaa gctgcgctgg ttacggaatg
     1741 ccgcgccctg gcacaacaaa atggcgggaa tattacgctg acggaagatg tagctaaagg
     1801 agttgaaggt gctgacttta tctataccga tgtatgggtg tcgatgggtg aagcaaaaga
     1861 gaaatgggct gaacgcattg cattgctgcg tgattatcaa gtgaacagca agatgatgca
     1921 gttgaccggt aacccggagg tcaaattcct ccactgcctg cccgcgtttc atgacgacca
     1981 aacgacgctt ggcaaaaaaa tggcggaaga gtttggccta catggcggaa tggaagtgac
     2041 tgatgaggtc ttcgaatctg ccgccagcat tgtatttgat caggcggaaa accgcatgca
     2101 taccatcaaa gcggtgatgg tcgcgacgct cagtaaataa ttgatatcgc tataaagatg
     2161 ccgggaaata caaaaacatc ccggcgtcac attattttat gcagtgatag ttcaattatt
     2221 ccaccccaat ttttttattg ctatacgtaa atcttccgga ggatttaatt ttcctaactg
     2281 ttcttcatta atatatccat atatattgat tttgccattc cggaggattt tcatcattct
     2341 tgttctaatt tttaattcat tacccatctt ttcttcaaaa atcttaacga cataacccgg
     2401 aagtttattt acaatttctt ttgttttttc ttcatcacca taaacatatc ctaactcatt
     2461 aagatacagc tgttcgccct cataacactt aagtaattga tagaagtacc acttagcaaa
     2521 atcaggttgc ttcatgaaaa aatccttcaa aaaaaatatc atttactaaa atgctgacag
     2581 gcacttcatt aaatagtaac ttaa
//
LOCUS       DAZRUF010000348         2591 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZRUF010000348 DAZRUF010000000
VERSION     DAZRUF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.351"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            9..1334
                     /gene="gdhA"
                     /locus_tag="ACHXOL_004123"
     CDS             9..1334
                     /gene="gdhA"
                     /locus_tag="ACHXOL_004123"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR6810943.1"
                     /translation="MESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYRQMSL
                     LERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVNLSILKF
                     LGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGADTDVPA
                     GDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTEAMLK
                     RHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTKEKLARL
                     IEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQLIANGV
                     KAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWKAEKV
                     DARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(1451..2491)
                     /locus_tag="ACHXOL_004124"
     CDS             complement(1451..2491)
                     /locus_tag="ACHXOL_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR6810944.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
BASE COUNT          676 a          622 c          613 g          680 t
ORIGIN      
        1 catattctct ggagtcattc ctcaaccatg tccaaaagcg cgacccgaat caaaccgagt
       61 tcgcgcaagc cgttcgtgaa gtaatgacca cactctggcc ttttcttgaa caaaatccaa
      121 aatatcgcca gatgtcatta ctggagcgtc tggttgaacc ggagcgcgtg atccagtttc
      181 gcgtggtatg ggttgatgat cgcaaccaga tacaggtcaa ccgtgcatgg cgtgtgcagt
      241 tcagctctgc catcggcccg tacaaaggcg gtatgcgctt ccatccgtca gttaaccttt
      301 ccattctcaa attcctcggc tttgaacaaa ccttcaaaaa tgccctgact accctgccga
      361 tgggcggtgg taaaggcggc agcgatttcg atccgaaagg aaaaagcgaa ggtgaagtga
      421 tgcgtttttg ccaggcgctg atgaccgagc tttatcgtca tctgggcgcg gataccgacg
      481 ttccggcagg tgatatcggc gttggtggtc gtgaagtcgg ctttatggcg gggatgatga
      541 aaaagctctc caacaatacc gcctgcgtct tcaccggtaa gggcctttca tttggcggta
      601 gtcttattcg cccggaagct accggctacg gtctggttta tttcacagaa gcaatgttaa
      661 aacgccacgg tatgggtttt gaagggatgc gcgtttccgt ttctggctcc ggcaacgtcg
      721 cccagtacgc tatcgaaaaa gcgatggaat ttggtgctcg tgtgatcact gcgtcagact
      781 ccagcggcac tgtagttgat gaaagcggat tcacgaaaga gaaactggca cgtcttatcg
      841 aaatcaaagc cagccgcgat ggtcgagtgg cagattacgc caaagaattt ggtctggtct
      901 atctcgaagg ccaacagccg tggtctgtac cggttgatat cgccctgcct tgcgccaccc
      961 agaacgaact ggatgttgac gccgcgcatc agcttatcgc caatggcgtt aaagccgtcg
     1021 ctgaaggggc aaatatgccg accaccatcg aagcgactga actgttccag caggcaggcg
     1081 tactgtttgc accgggtaaa gcggctaatg ctggcggtgt agcaacgtcg ggcctggaaa
     1141 tggcacaaaa cgctgcgcgc ctgggctgga aagccgagaa agttgacgca cgtttgcatc
     1201 acatcatgct ggatatccac catgcctgtg ttgagcatgg tggtgaaggt gagcaaacca
     1261 actacgtgca gggcgcgaac attgccggtt ttgtgaaggt tgccgatgcg atgctggcgc
     1321 agggtgtgat ttaagttgta aatgcctgat ggcgctacgc ttatcaggcc tacaaatggg
     1381 cacaattcat tgcagttacg ctctaatgta ggccgggcaa gcgcagcgcc cccggcaaaa
     1441 tttcaggcgt ttatgagtat ttaacggatg atgctcccca gggaacattt cttatgggcc
     1501 aacggcattt cttactgtag tgctcccaaa attgcttgtc gtaacgataa cacgcttcaa
     1561 gttcagcatc cgttaacttt ctgcgatagc agcagatatg ccagtaaaga aaccccatta
     1621 gactattttt ttgataatct tcttcgcttt cgaacaactc gtgcgccttt cgagaagcaa
     1681 gcattatata atgccaggcc agttcttctt caattgtccc gttttgaaaa gctgtgcttg
     1741 atatcgaggt catccatgat aattccgccg cccatattag cttcgccgag gatttaccgg
     1801 agcgattatt agcgcaatca gagatatagt ctgagggaaa aacagcaaat ttattcaaca
     1861 aggcgataac ctgctctggg gcttcctcca tgtttgcttt aaaggtattg gctccatggt
     1921 cgccagaaag aaaatgctcc attaaggcac aataactttc gctatcttcg ataccccatt
     1981 gattctctaa agactcgcgt cttttactga tgatatcgat cgagtcaaaa ggaagcacat
     2041 gatattggaa ggtatcttta ccaggttcag gctttcgcgg ccagaactct agcgtttcag
     2101 accattgctt atgatagaat cgataaggtg cgatcaattg tagcgcctgt aacttctcga
     2161 tactgagcgg ctcaatacct ttagcctgat aataatgcag ttgttctttt tttgctttaa
     2221 aaccggcccg aacaataagc cccatcataa ttaatagata aagaaaagag catcccgcgg
     2281 taatcaggcc tctttcattc aaaccgttgg atgttatcgc tgcgaacaca aacattacag
     2341 cgacaacaca tgttaaataa aacccccact tacaaagcag catggcctta ttttctttaa
     2401 tcatccgttc aaaattacta ttaaatattt cccagccatt aaaagaatac ttctcgctcc
     2461 caggatggtt ttgtaataaa acttttttca tcacattccc tgttattaca tactttagag
     2521 gcgataattt tatccatgca aaaaaaatat ccaacgaatt ttcttgatcc gctggatatt
     2581 tcagaatatg a
//
LOCUS       DAZRUF010000349         2564 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZRUF010000349 DAZRUF010000000
VERSION     DAZRUF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.352"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            206..829
                     /gene="nfeR"
                     /locus_tag="ACHXOL_004125"
                     /gene_synonym="yqjI"
     CDS             206..829
                     /gene="nfeR"
                     /locus_tag="ACHXOL_004125"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR6810945.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(936..2456)
                     /gene="aer"
                     /locus_tag="ACHXOL_004126"
     CDS             complement(936..2456)
                     /gene="aer"
                     /locus_tag="ACHXOL_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR6810946.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
BASE COUNT          592 a          722 c          646 g          604 t
ORIGIN      
        1 gcgatttgta gaactgatat gtctatagtc tgataagacg aaccgcctct tctcaggcat
       61 cattactcaa cgccggatgc ggcgtgaacg ccttatccgg cctacgtgtg agatgagtcc
      121 acatttaaaa atcattttta ttcttgcaag agtgctcata tcagatataa attagatata
      181 tctaatcaag caaaaggagg ctgatatgag ccatcatcac gaagggtgtt gtaaacatga
      241 aggccagcca cgccatgagg gctgctgcaa aggtgagaag tcagaacacg agcactgcgg
      301 acacggtcac cagcatgaac acggtcaatg ctgcggtggt cgccacggtc gcggcggcgg
      361 tcgtcggcaa cgtttctttg gtcacggtga attgcgtctg gtgattctgg atattctctc
      421 gcgcgatgac agccacggtt acgaattgat taaagcgatt gagaatctaa cccaggggaa
      481 ttacacccca agcccgggcg tcatctaccc aacgctggat tttctgcagg agcagtcgct
      541 gattaccatc cgcgaagagg aaggtggtaa gaagcagatt gcgctgaccg aacaaggcgc
      601 gcagtggctg gaagaaaacc gcgaacaggt ggagatgatt gaagaacgca tcaaagcgcg
      661 ttgcgttggt gctgcgctgc gccagaaccc gcaaatgaag cgggcgctgg ataattttaa
      721 agcggtgctg gatttacgcg tcaaccagag cgatatcact gatgcacaaa taaaaaagat
      781 cattgcggtg atcgaccacg ccgcttttga tattacccaa ctggattaat caccgattgc
      841 cggatgcggc gtaccctaca caccccgcag gcctgataag atgcgccagc atcgcatcag
      901 gcattgtgct ccaaccgccg gatccggcat accgattaat gcagcaccgt caccgcgtct
      961 tccagtcggc tggcgcggtg tttcaccatc gccgacacct gcgcactctc ttccaccagc
     1021 tcagcatttt tctgggtgat caggttaagc tcatccactg cacgggtcag gctggaaagc
     1081 ccatcggcct gttccagcgt tgaatggcta atctgggcga tcaactgggt gacgtttttc
     1141 acctgtgcca caatatcttc catcgtccgt ccggcggcgt gtacctgctg cgaaccggat
     1201 tgcaccttat cagcactggc atcaatcagc ttgcgaatat cgttggcagc attagcgctg
     1261 cggctggcta aatgacgcac ttcccctgcc accactgcaa aacctttgcc ctgttcgccg
     1321 gcacgcgccg cttccaccgc cgcattcagg gccagaatat tggtctgaaa cgcaatatcg
     1381 ttaatcagcg aagtaatggt gccaatgcgc tgggtactgt cggcgatatc gtccattgtc
     1441 ttgatcaccg tggtcatcgc ctccccaccc tgcactgccg cattactggc ggtgatagaa
     1501 agtttatcgg cagccgacgc cgtggcagag ttctgtttca ccgacgccgc catttggttc
     1561 atggtcgcca ccgtttgctg aacgttatca actgtctgct gggtatgttc gttcagttca
     1621 tcggtgcctt tcgccagcgt ctcactgcca tttctgacac tggacacctg gcttgagaca
     1681 tcgttaatta gccaacggca catcaggcca agctgcccta ccgcgcgtaa tgtcagcccc
     1741 agctcatcgc tgcgattcag gtgctcaaca ctattacgct ctccggtcgc caccttcagt
     1801 gcctgacggg cgacattttc tatcgggcgc acaatctgcc attcaaaaca agcgcttgcc
     1861 aacaatacca ctaacacaca gaggaaatac gtcaccaccg gggcagcaac aaaccaaagc
     1921 atggccgcca gcaagataaa catcagggtc atcactccac gcgcccgcca gcgaagcggt
     1981 aatgaaggca gtttacccag ccagccttta cgcaccacca ggcctttatg aatacgctta
     2041 ctggtacgtc cggcgttcag cgctttgtac agcggctcca ccgccgcaat ctcttcatcc
     2101 gtcgcccggg tacgaatcga catatagcca ctgatttttc cctcgcgcac catcggtacc
     2161 gcattggccc gcacccaata atggtcgcca tttttgcggc gatttttcac gatgccgctc
     2221 cagggctccc cttttttcag ggtgaaccac atatccgcaa acgccgcttt tggcatatcc
     2281 gggtgacgca ccatgttgtg cggctgccct tgtaactctt gcaaggtaaa gccgctcacc
     2341 tgcacaaaag tgtcattagc atgagtgata tagctttgca gatcggtagt ggacatcaga
     2401 gtggtatcgt ccgccagcgg ggtattttgc tgggtgacat acggatgaga agacatgttc
     2461 gcgtcctgtg caggttatat ggttgttaac ttcttgtcag agtttatgtc ggccccgctg
     2521 cggttatctt taaccgatta atttgattta gatcgcaatt tgcg
//
LOCUS       DAZRUF010000350         2561 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZRUF010000350 DAZRUF010000000
VERSION     DAZRUF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.353"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(1..803)
                     /locus_tag="ACHXOL_004127"
                     /pseudo
     CDS             complement(1..803)
                     /locus_tag="ACHXOL_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(856..1452)
                     /locus_tag="ACHXOL_004128"
     CDS             complement(856..1452)
                     /locus_tag="ACHXOL_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR6810947.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1479..2081)
                     /gene="yadL"
                     /locus_tag="ACHXOL_004129"
     CDS             complement(1479..2081)
                     /gene="yadL"
                     /locus_tag="ACHXOL_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR6810948.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2096..>2561)
                     /locus_tag="ACHXOL_004130"
     CDS             complement(2096..>2561)
                     /locus_tag="ACHXOL_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6810949.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          712 a          552 c          513 g          784 t
ORIGIN      
        1 aagcatagcg gtaaaacaag tgggattagt caacttgaca ttgagcagat cgaaatcgat
       61 tttgataaaa taactatgct catttatacc ttggcctgcg ccataggctt taaatgaata
      121 cagataattt gatgagctgg ataatttgat ctgttgagtt actgttggat caaagtttgt
      181 atctgtatag aattcaattg taagggtctg ataaatacca cctacagccc agtatttacg
      241 ggtgctattc gcattattgc aactccaata taacacgtct tcgcttggat tataccacga
      301 aaaatattgt tctgcagagt caccaatgta tatcccaggt gaactaacgt tagttacggt
      361 accgtaagct gaccacatgt ttgaaattaa taatgtgtaa tacaaaccag gaactgatgt
      421 attaaataat ttatgggagc catacatttt acctgagtca accattgcat tttcaatacg
      481 cattgcgcta ccacttgtgc tatcacttct gttgcaataa agaaaaccac caccagaagc
      541 ggtatctaac tgactccact gtaattttac acctgtaggt ccaagctgcg tcggtttgta
      601 gaaaactaaa ttatttgtac ttgttgcata tctgaatatg acagtagcat cgccattcgg
      661 ccccgtttct ctccattttt gatctgacgg accaacgccg taactattcc caacaatata
      721 tcctacgtta tcatcagtgc cagcactcgc ggtataacaa ctcaagcaca gaattaaaaa
      781 aaatgtctga agaatttctt cattatcact ccaccatcta ctgctgtaac agtcgcagtt
      841 atttgttttg atctattatt cgtagtacac atcaactaat atatgactca ttacttggcc
      901 agatatgaca tccagcgcgg gatcgatttt ttgcattctg gcataaaatt tccatgaaga
      961 attatgcagg gcagaagtca ttactggata cacggaacga gaatttcctg ctgaactaat
     1021 aacattgaat atattggtcg ggtcctgaat ggaaaagaga acgacaccga catttttagc
     1081 cccggttgcg acttcattgg caaatatttg ccttgaacct gcagccatga caccgctaag
     1141 ggctctaaaa tcaatatgta actgattaag cacatcgcca gttccctgta atgcacagtc
     1201 tgatactgtg acagtgaact cttttccacc tggttgatag ctttcaggag taacattatc
     1261 tgaaaaatat cccaccccaa cgacgccaag atcaatatta ccctggttac tgattccgct
     1321 tttgcaagtg ctgtttttta cattggcaac aaggtcaata tcctgcccag cgatggctgg
     1381 ataggtaatg ggaaactgta gtgacagaaa cagaattatt ctcttcatca gcttacgttg
     1441 agtgggatgc atatatagtt cctggatctg tcagtctgtt agttataggt tactgtaaat
     1501 gtggcaggtg cagaaaattt acccgcagtg acattgctta cggttttatt tttttcgacg
     1561 accagtcgag cactcatcgg ataataaaca accgcgttgg ctgcattgga gatagtgacc
     1621 tcttgagatg ctggtgtcac acaactaaat tgtgttgcgc tccctgttgc cggagtttcg
     1681 gtgctccaga cttcaacggc gacggcactt gctttatctg cggctgtgga tccatttgcg
     1741 agccccgcac cgtcattagc agtaccttcg catgtggctc gcttggttaa ttttatttgt
     1801 gcttttttat tggggatacc cgcacagtct ttgaatttga gtttgaatgt tttaactttg
     1861 gtcttggcat tgatttcaga aatatataca tcgccaaagt caacaacaga aatgtcctga
     1921 tcagagtcat ttactaatgt agcggtacaa gtacctattt ctacggtagt aatcacggtc
     1981 agcccaatgc tgtctgttgc cgcataagag ggtacgctga acaatgaggc agccaaaacg
     2041 acactgctgg ataatccata acgtaaattt ttaaatgtca tcttaattat ccttcttatt
     2101 gatagatgaa attaaacgtt gccagtgcac gaaaattccc cggtacgcct tgccctgcat
     2161 ctgtttcacg tagcgcaacg gtcatttcaa gacccttatc gggctgcatc tcgtctgtgc
     2221 tccagcgtat cttttccgca ctgttaggtt taaggaaagt ggcatcatca gtagtccgtt
     2281 ttttgaaacc cataccgata ttacttgtcg tcgaagatga atcaccagac tgcggtataa
     2341 taagcttagg tgagcttgat gatgcatttc cggtcagagt ggtatcaatc caactgatgc
     2401 cactgctgca cccactagca accagtttaa aatcagcctg agattctgtc gttttattcg
     2461 cgatcttatc cagacccatc ttagggattc tcaatgtgta gttattattg ccatcattcg
     2521 tgacgttatt accagtaagt gtgatgttac aggttgtcgc t
//
LOCUS       DAZRUF010000351         2561 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZRUF010000351 DAZRUF010000000
VERSION     DAZRUF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.354"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(34..108)
                     /locus_tag="ACHXOL_004131"
     tRNA            complement(34..108)
                     /locus_tag="ACHXOL_004131"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74..76),aa:Gln,seq:ttg)
     gene            complement(132..216)
                     /locus_tag="ACHXOL_004132"
     tRNA            complement(132..216)
                     /locus_tag="ACHXOL_004132"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Leu,seq:tag)
     gene            complement(225..301)
                     /locus_tag="ACHXOL_004133"
     tRNA            complement(225..301)
                     /locus_tag="ACHXOL_004133"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(265..267),aa:Met,seq:cat)
     gene            complement(681..2345)
                     /gene="asnB"
                     /locus_tag="ACHXOL_004134"
     CDS             complement(681..2345)
                     /gene="asnB"
                     /locus_tag="ACHXOL_004134"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR6810950.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(2299..>2561)
                     /locus_tag="ACHXOL_004135"
     CDS             complement(2299..>2561)
                     /locus_tag="ACHXOL_004135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810951.1"
                     /translation="PVKYNHSIKPAKNYRFINKYILTPAPFSPIDNHHYVFYFFTAKS
                     LAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
BASE COUNT          618 a          645 c          637 g          661 t
ORIGIN      
        1 taaccggtcg gtgaaccgct tactcgaaga agatggctgg ggtacctgga ttcgaaccag
       61 ggaatgccgg tatcaaaaac cggtgcctta ccgcttggcg ataccccatc cgtacaacgc
      121 tttctggtga atggtgcggg aggcgagact tgaactcgca caccttgcgg cgccagaacc
      181 taaatctggt gcgtctacca atttcgccac tcccgcaaaa aagatggtgg ctacgacggg
      241 attcgaacct gtgaccccat cattatgagt gatgtgctct aaccaactga gctacgtagc
      301 catctttttt tcgcgatacc ttatcggcgt tgcggggcgc attatgcgta tagagccttg
      361 cagcgtcaac ctctttttca aggaaaattg ctcgaaagtg actgtttggt taggttgcga
      421 acagcgtggc gctatattcg tcaattattg tttactttgt gttttttccc caccctacag
      481 ccattctttt gtcatacagg atgaaattcg gaattcaaca atggtggtgg gaaattaatc
      541 tatgaaatac tggcctacag tgatgagttg tcaaacagtg atgtggcaaa cccggaacat
      601 ttccttactg catatcagaa tcaacaagca cctcaataac tgaaacagcc ccggatttca
      661 ccggggctgt ttcgcatttc ttacttatac gccgactggt gaacgccaac cgcacgacca
      721 gacggatcgt ccattttctt gaacgcctca tcccactcaa tcgctttagc ggaagaacac
      781 gcgacggacg ggccgcccgg cacgcactca gcggcgctcg gaagcgggaa cagttcttca
      841 aagatctccc ggtacagata cgcttctttt gaggttggcg tgttgtacgg gaagcggaag
      901 cgggcagttt ccagttgctg atcagaaacc tgctgcgccg ccacttcttt cagggtgtcg
      961 atccaactgt aaccgacgcc atcggagaac tgctctttct gccgccaggc cacgcttgcg
     1021 ggcaggtatg actcaaaaca ttcacgcagg atgtgttttt ccattttgcc gttaccgcac
     1081 attttatcct gcgggttaat gcgcatcgcc acatcaagga attttttgtc gaggaacgga
     1141 acgcgtgctt ccacgcccca ggctgacatc gctttgttgg cgcgcgcgca gtcatacata
     1201 tgcagggcca gcagtttacg caccgtctct tcatgcagtt ctttagcgtt gggcgcttta
     1261 tggaagtaaa gataaccgcc aaacacttca tcagaacctt caccggacag caccatttta
     1321 atgcccatcg ccttgatctt acgcgacatt aaatacatcg gtgttgaagc gcgaatagtg
     1381 gtcacatcat aagtttcgat gtggtaaatc acgtcgcgga tggcatccag accttcctgt
     1441 acagtgaagt gaatttcgtg atgcaccgtg cccagatggt ttgccacttc ctgggctgct
     1501 ttaagatccg gtgaacccgg cagacctaca gcaaaggagt gtaactgggg ccaccaggct
     1561 tcagagcgtt cctgatcttc cacgcgacgg gctgcgtatt tcttggtgat agcggaaata
     1621 attgaggaat ccagaccacc agaaagcagc acaccgtaag gcacatcaga catcagatgg
     1681 cttttaacgg aatcttccag agcctgacgc agctcgtttt tgtcggtcac gttatctttc
     1741 actgcatcaa agtcgaacca gtcgcgatga taataagaac ggatttcgcc gtcctggctc
     1801 cacaaatagc tccccgccgg gaactcttta atcgtgcggc aaactggcac cagcgatttc
     1861 atttctgagg ccacatacag ctgaccgtgt tcgtcatacc ccatatacag tgggatgatc
     1921 cccagatggt cgcgaccaat caggtaggca tctttttcgc tgtcgtacag ggcaaaggca
     1981 aacatgccct gcaagtcgtc gagaaattcc ggcccttttt cctgatacag cgcgaggatc
     2041 acttcacagt cagaccctgt ctggaactga taacgatcgc catattcagc gcgcaatgcc
     2101 tggtggttgt agatttcacc gtttaccgcc agtacgtggg ttttttgttg gttgtagaga
     2161 ggttgcgccc ccgcgttaac gtcaacaatt gacagacgtt cgtgagcgag aatggcgtta
     2221 tcgctggcat aaataccgga ccagtccggg ccacgatgac gcatcaggcg tgacagctcg
     2281 agtgctttct tacgctgctc aactgcgtct gttttgatat cgaatacgcc aaaaattgaa
     2341 cacataacct tctccgttaa cctggtattt gttgcttgtt gtgtttgctt gtttaaaaaa
     2401 atgccgcaaa gcagcactgt gcgcaagcga tttggcggtg aaaaaataaa aaacgtaatg
     2461 gtgattgtcg attggtgaaa aaggggctgg tgtgaggatg tatttattga tgaatcgata
     2521 atttttagcg ggttttattg aatggttata ttttacgggg g
//
LOCUS       DAZRUF010000352         2559 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZRUF010000352 DAZRUF010000000
VERSION     DAZRUF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.355"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..2548
                     /locus_tag="ACHXOL_004136"
     CDS             <1..2548
                     /locus_tag="ACHXOL_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HFR6810952.1"
                     /translation="TFPTLTINSEANNFETYDSDYRTVLLSDKSVAPFDIVVNYLPDD
                     PTHQFLDNAAKSQQLFQIIIQYQMNKDENTITYAMVNGYITSYKLDGDKGSVVTKAYS
                     FAPHDVIARAMTINALSPIYQGDYGLGSNTTDVPQYSPSVPTGNSFIKVPSSQAGNPA
                     GADLMGVGLVDGTTVSSLAMTKTGTLSIYAKNATTAWTRIYTATQMDGRYVPLTRTIN
                     GHALTANLVLNSEDTGSVPDTRTVNGKPLSEDVVLTSTDTGSVPDTRTVNGHVLSEDI
                     VLSSEDTGSVPDTRTVNGHVLSENVVLSSEDTGSVPDTRMVNGHVLSEDVVLTSTDVG
                     LGKVTDDAQLTIAGNLSDLGDVEEARINLAVYSMDEVDAEVTALADSITSLDSASMKK
                     ASNLSDLSSLSTARTNLQVNRLTQGATYTDLMSNDLTNRIRIQDNGAWGASTGTGWIA
                     LGIGQGGTGATDALSARLNFQVDRFNQGSNLTTIKSADSSAALVVYDGGQWGSATYIA
                     GAFTGWKALAIPQGGTGVKTQAELWPAIRPTGATSLAGDPVSDSDATTKRWVENLLSS
                     RTSVSPQWDVIASSPSSTAIGSYVTGGRIRSICNIGTSTEISGGFRVGKTMGQTGQAD
                     LTLFISENGAEDKLWGLKGPTGTILTDVQSGWMTNTAVSGPTDANTITFNGMYAGPGD
                     SGINYSEKYSPLLHMQRYPGANRSQLQIDGGGTLRVRGGNGTTWFGWLVMQAQGTSGR
                     EFKRDITPANPVEALDRIMSQDLVTFIYKDDEQERVRFGVIAEDAELTAPQYIKHDEE
                     TVEIEGEIVTRDHPRVDLNPIVMDLMGAIQAQQEEINQLKDLVQQLLNNK"
BASE COUNT          748 a          508 c          617 g          686 t
ORIGIN      
        1 tacttttcca accttaacaa taaattcaga agcaaataat tttgaaacgt atgatagtga
       61 ttacaggaca gtactactat ctgataaatc agtagcacca tttgatattg tagttaacta
      121 cctacctgat gatcctacac atcaattctt ggataatgcc gctaagtctc agcagctttt
      181 ccagatcatc attcagtacc agatgaataa ggatgaaaat accattactt atgcgatggt
      241 gaatggttat atcacctctt ataagttgga cggtgataaa ggttcagtag taaccaaggc
      301 atacagtttt gccccacatg atgtgatcgc acgtgcaatg accattaacg cactatcgcc
      361 aatctatcag ggggactacg gtttaggttc gaacactacg gatgtacctc agtactcacc
      421 atcagtacct acgggtaaca gcttcatcaa agtaccatca tctcaagcag gcaatcctgc
      481 cggggcggat ctgatgggag tagggcttgt agatggcact actgtaagtt ctcttgcgat
      541 gacaaagacg ggtactctaa gtatctatgc aaagaacgct acaaccgcct ggacacgcat
      601 atataccgct acacagatgg acgggcgata cgtacccctt acacgtacca tcaacggcca
      661 tgcactcacg gccaatttag tactgaacag tgaagataca ggttcagtac ctgatacacg
      721 tactgtgaat ggtaagcctc taagtgagga tgtagtactt accagtactg ataccggctc
      781 tgtaccagat acacggacag ttaatggtca tgtactcagt gaagatatag tactgagtag
      841 tgaagataca ggttcagtac ctgacacgcg tacagtgaac ggtcatgtac ttagtgaaaa
      901 tgtagtactg agtagtgaag atacaggttc agtacctgat acacgtatgg tcaacggtca
      961 tgtactcagt gaggatgtag tacttaccag tactgatgtt ggcttaggga aggttacaga
     1021 tgatgcccag cttacgatag caggtaatct aagtgatctg ggggacgtgg aagaagcacg
     1081 tattaacctt gctgtttact caatggatga agtagatgca gaggtaacgg ctttagctga
     1141 cagtattact tcccttgatt ccgcttccat gaagaaagcg agtaaccttt ctgatctatc
     1201 cagtctctct acagcacgta ctaacctaca ggtgaaccgt ttgactcagg gtgctacgta
     1261 caccgatctg atgagtaatg acctgactaa ccgtatccga attcaggata acggggcatg
     1321 gggtgcaagt actggtactg gatggattgc tttaggtata ggtcaggggg ggacaggggc
     1381 tacagatgct ttgagtgctc gcctaaactt tcaggtggat cgctttaatc aaggtagtaa
     1441 tttaacaact attaaatccg ctgattcttc cgctgcatta gtggtttatg atggagggca
     1501 atggggttca gctacatata ttgcgggtgc tttcactggc tggaaagcat tagcaattcc
     1561 gcagggcggt actggtgtta agactcaggc tgagttatgg cctgcgattc gtcctacggg
     1621 tgcaacttct ttagcaggag atcctgtctc tgattctgac gcgactacca aacgctgggt
     1681 tgaaaacttg ctgagtagtc gtacttcagt tagtcctcaa tgggatgtta ttgcttcttc
     1741 cccttcctct acggcaattg gtagttatgt aactggaggg cgtattcgtt cgatatgcaa
     1801 cattggtaca tcaactgaaa tatctggtgg ttttcgggtt ggtaaaacga tgggacagac
     1861 tggacaggct gatctcaccc tttttatttc tgaaaatggt gcagaggata aactatgggg
     1921 gctaaaaggg ccgactggaa cgatcctaac tgatgttcaa tcaggatgga tgactaatac
     1981 cgctgtttcg ggaccgacag atgccaatac catcaccttt aacggtatgt atgcagggcc
     2041 aggtgactca ggtattaact atagtgaaaa atattcccct ttattgcaca tgcaaagata
     2101 cccaggggca aatcgctctc agcttcagat agacggtggt ggtactttaa gagtacgagg
     2161 cgggaacggt acaacatggt ttggatggtt agtaatgcaa gctcaaggta catcaggtcg
     2221 tgagtttaaa agagacataa ctccagcaaa tccagttgaa gcattagatc ggataatgtc
     2281 acaagattta gtaacgttta tctacaagga tgatgaacaa gaaagggtta ggtttggtgt
     2341 tatcgccgaa gatgctgagt tgactgctcc gcagtacatt aagcatgatg aagaaacagt
     2401 tgaaatagaa ggggagattg tcactcgtga tcatccacgt gttgatttaa atccaatagt
     2461 gatggacttg atgggagcta ttcaagccca acaagaagaa attaatcagc taaaagattt
     2521 agtacaacag ctattaaata ataaataaat ataaacggg
//
LOCUS       DAZRUF010000353         2555 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZRUF010000353 DAZRUF010000000
VERSION     DAZRUF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.356"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            137..469
                     /locus_tag="ACHXOL_004137"
     CDS             137..469
                     /locus_tag="ACHXOL_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR6810953.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            616..1977
                     /gene="yegQ"
                     /locus_tag="ACHXOL_004138"
     CDS             616..1977
                     /gene="yegQ"
                     /locus_tag="ACHXOL_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR6810954.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2307..>2555)
                     /gene="yegR"
                     /locus_tag="ACHXOL_004139"
     CDS             complement(2307..>2555)
                     /gene="yegR"
                     /locus_tag="ACHXOL_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR6810955.1"
                     /translation="ETSIGKFGLAYKSNIQRKLDNQYYTEAEASLARGRISGAENIVK
                     NDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE"
BASE COUNT          662 a          665 c          616 g          612 t
ORIGIN      
        1 atcaataagt tggagtcatt accaactgac attatctctc cgccctggca ggcatcagaa
       61 aatcgctcat actttaatcg gtaaacagca cctttagatg ctgttttcga tacgcaattt
      121 caatcaagga gtcattatgg ctggttggtt tgaactcagt aagagcagtg ataatcagtt
      181 ccggtttgtg ctaaaagcgg gcaatggtga gactatcctc accagcgagc tgtatacatc
      241 aaaagcctct gcggaaaagg gcatcgcgtc ggtgcgtagc aacagcccgc aagaagaacg
      301 ctatgagaaa aaaacggcaa gtaacggcaa attctatttc aatctgaaag ccgccaatca
      361 tcaaattatc ggctccagcc agatgtacgc caccgcgcaa tctcgtgaaa ccggaattgc
      421 ctccgttaaa gccaatggca caagccagac ggtgaaagac aatacgtaat tacgatgccg
      481 ggcgtgggtt gtcggcgtcc ggcgctttac cagatccccc cacagcgcta caatgcccgc
      541 ccttaaagtg ggggcactcc cctaaccgct tcatcaggtg aagcggagct gacctgtcat
      601 cagaacgaga gaattatgtt taaaccggaa ctcctttccc cggcgggaac gctgaaaaat
      661 atgcgttacg ctttcgctta tggcgcagat gctgtttatg cgggccagcc gcgttactcc
      721 ctgcgtgtgc gcaacaacga attcaaccac gaaaatcttc agctcggcat caatgaagcc
      781 cacgcgctgg ggaaaaagtt ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg
      841 aaaaccttta tccgtgacct gaaaccggtg gtggaaatgg ggccggatgc gctgattatg
      901 tccgatccag ggctgattat gctggtgcgt gagcacttcc ctgaaatgcc gatccacctc
      961 tcggtgcagg ctaacgccgt aaactgggcg acggtgaaat tctggcagca aatgggcctg
     1021 acccgcgtga tcctctctcg cgagctgtcg ctggaagaga ttgaagagat ccgcaatcag
     1081 gtgccggata tggagattga gatcttcgtt cacggtgcgc tgtgcatggc ctactccggt
     1141 cgctgcctgc tctctggcta tatcaacaag cgcgatccga accagggtac ctgcaccaac
     1201 gcctgccgct gggagtacaa cgtccaggaa gggaaagaag atgacgttgg caacatcgta
     1261 cacaagtacg agccgattcc ggtgcaaaat gttgagccga cgctaggtat cggcgcgcca
     1321 accgacaaag tgtttatgat cgaagaagcc cagcgtccgg gcgagtatat gaccgcgttt
     1381 gaagatgagc acggcactta catcatgaac tcgaaagatc tgcgcgccat cgcccatgta
     1441 gaacgcctga ccaaaatggg cgtgcattcg ctgaaaatcg aaggccgtac taaatctttc
     1501 tactattgcg cacgcaccgc acaggtttat cgtaaagcta tcgatgatgc cgctgcgggc
     1561 aaaccgttcg ataccagcct gctggaaact ctggaaggtc tggcgcatcg tggctatacc
     1621 gaaggtttcc tgcgtcgtca tactcacgac gattatcaga actacgaata cggttattca
     1681 gtttctgacc gccagcagtt tgttggtgag tttaccggtg agcgcaaggg ggacctcgca
     1741 gcggtagcgg tgaaaaataa attctccgtt ggcgacagcc ttgagctgat gacgccgcaa
     1801 ggcaacatta actttaccct tgagcacatg gaaaacgcca aaggtgaagc gatgccggtc
     1861 gcaccaggcg atggttatac tgtgtggctc ccggtcccgc aggatcttga gctaaattac
     1921 gcgctgctga tgcgtaattt ctccggggaa accacgcgta acccacacgg taagtgatta
     1981 atttcgatta tttttcccgg atggaaaatt cttagaaacc gatcacatac agctgcattt
     2041 attaaggtta tcatccgttt cgctgaaaaa cataacccat aaaatgctag ctgtaccagg
     2101 aaccacctcc ttagcctgtg taatctccct tacacgggct tattttttat gcataagccc
     2161 tatccctggt caccgtcttc cattgaccac atcgatagaa tctcccttca tagcacgatg
     2221 cctttcacgt aacggcatcg tgctcgcaca ggttccggct aagcacaacc agaacgcgca
     2281 tgtttgatgc ttaccaaaaa atattctcac tctccacatt tgaatgtcag acgagcgacg
     2341 ccatgtaatc ctgcaccttc tgtcttcagg tcaactatct gcattttttt gccctgagta
     2401 acacagaaat gggctgcatc attttttact atattttctg caccagatat tctgccccta
     2461 gctaaagaag cttcggcttc ggtgtagtat tggttatcga gtttacgctg aatattactt
     2521 ttatatgcaa gaccaaattt accgatactt gtttc
//
LOCUS       DAZRUF010000354         2548 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZRUF010000354 DAZRUF010000000
VERSION     DAZRUF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.357"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..82
                     /locus_tag="ACHXOL_004140"
                     /pseudo
     CDS             1..82
                     /locus_tag="ACHXOL_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            829..1674
                     /locus_tag="ACHXOL_004141"
     CDS             829..1674
                     /locus_tag="ACHXOL_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR6810956.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPKSFTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQSQDWPGSVMTGCSNPVRLTTHEIATSGGELIKFIAEAAIMATT
                     LSHPCVTVENGRAVTTSVAVAEFFHKRHDNVLRAIANIECSDKFTALNFEASEYTDSI
                     GRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPG
                     DGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQL
                     LKIHC"
     gene            1685..1873
                     /gene="dicB"
                     /locus_tag="ACHXOL_004142"
                     /gene_synonym="ftsT"
     CDS             1685..1873
                     /gene="dicB"
                     /locus_tag="ACHXOL_004142"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR6810957.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1861..2061
                     /locus_tag="ACHXOL_004143"
     CDS             1861..2061
                     /locus_tag="ACHXOL_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR6810958.1"
                     /translation="MCTMNTVFALVLTVFLVSGEPVDMVTGVYSSMKECMTAAAKQKI
                     PGDCYPVDKVIHHDNNEIPAGL"
     gene            2146..2421
                     /locus_tag="ACHXOL_004144"
     CDS             2146..2421
                     /locus_tag="ACHXOL_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810959.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          745 a          557 c          599 g          647 t
ORIGIN      
        1 agatgcaaaa cgcctccagc aactggagcc aaatgcaggc actggggcac gcatctggct
       61 ggctaaagaa gcgctggaat ctggcgatta tgatagcgaa gaagccttct acaaagcaga
      121 aggccgtaca ggatattcac cgggtcttgg cggggtataa ataccatgcg cattgactga
      181 attcgcaaac aaaaacagac gcgcgatacc tggatagtcg gtctgcatag tcagatatct
      241 ggctgcgaat ttgataaata tcaccgccct tttcggaaac ataagttccg tccgggagtt
      301 gattatacga accatctcca tgggggatcg ttctcctgaa tcctagtgag agcatatatt
      361 tatgaagccc ttcgtaatcc tctggctcag cattatatag ttctactctg gcgagatacg
      421 ttggcatatt catttcctta ctggttgtgt gagaacttca gtaaagatac caccaaagcc
      481 cggaggtggt gaaataaaac cgggcacaac acgaaggcgc atttccggta tccataaaga
      541 gtcggtcttg tctgttaaat ttaaatggtg ggagtgcgcc tccggttgta aataacgaca
      601 ttgctgtgtg aagtaccagt tggcggcatc ggtttaattg ctggctgatg tccgcccttt
      661 ttaaagtgaa ttttgtgatg cggtgaatgc ggctcagcgc acgcggaaca gttaaaacag
      721 taaggaaaat cccttattcc ggcgttaatt gttaactggt taacgtcacc tggaggcacc
      781 aggcaccgca tcaacaaagt tcacttcggt gatgaaagga aagagaaaat gttgaatgta
      841 gctattgaaa accagaacgg gtggaattat agtgcacctg cacctcataa aacgggtgcc
      901 gggattgcta ccccgaagtc attcacggcg cataaccgcg ctcaggcggt ttttttatgc
      961 gtaatgcaca gccacattca aattatggtg gggcgtgcgg ggcagtcgca agactggccg
     1021 ggttccgtga tgaccggttg tagcaaccct gtacgtctca ccacccatga gattgctacc
     1081 tccggtggtg agttaatcaa attcatcgcg gaggctgcca tcatggctac taccctttct
     1141 cacccttgtg taaccgtaga aaacggtcgc gctgtcacta cgtctgttgc ggttgcagag
     1201 ttcttccaca aacgacacga caatgtgtta cgtgccatcg caaatattga atgctcagat
     1261 aaatttactg ccctcaattt tgaagcgagc gaatacaccg actcaatcgg gcgcaaactc
     1321 ccaatgtacc aaatcaccaa aaacggcttc gttttcctgg ttatgggctt cactggcaaa
     1381 aaagccgctg cattcaaaga agcctacatt gctgagttcg accgcatgga ggccgaactg
     1441 cgccagaata acaccccacc tgctgacaag atgattccgg gtgatggtcg cactctggtt
     1501 gttcacttcg acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg
     1561 gtctgtaccc tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac
     1621 cggggcgcaa ttaaaaatat gactgtggaa caattactca aaattcattg ttgaggacgc
     1681 aataatggag acgttattac caaacgttaa tacgtctgaa ggttgttttg aaattggtgt
     1741 cactatcagt aaccctgtat ttactgaaga tgccattaac aagagaaaac acgaacggga
     1801 gctattaaat aaaatatgca ttgtttcaat gctggctcgt ttacgtctga tgccaaaagg
     1861 atgtgcacaa tgaatacagt atttgcactc gttctgacgg tttttcttgt ttccggtgaa
     1921 ccggttgaca tggttactgg cgtatacagc tcaatgaaag aatgtatgac tgccgcagcg
     1981 aaacagaaaa ttcccggtga ctgttatccg gtcgataaag ttattcacca tgataataac
     2041 gaaatcccgg caggtcttta aaacagttcc gtaataaata tccggtttca ttcttatatg
     2101 ccagcaatgg caggggttcg ctcaacctta attaaggaga aaaacatgat taccagttat
     2161 gaagccactg ttgtaactac tgatgacatt gttcacgagg ttaatctgga aggaaagcgt
     2221 attggctacg tgattaagac agaaaataaa gaaaccccat tcactgtggt tgatatcgac
     2281 ggtccatcag gcaacgttaa aacacttaac gatggtgtaa aaaaaatgtg tctggtgcac
     2341 ataggaaaga atctgcccgc agaaaaaaaa gccgaatttc tggcaactct gattgcaatg
     2401 aaattaaaag gtgaaatctg aaaaaaagaa agcctgcaca ctgtgcaggc ctgagtgaag
     2461 aacctgggac atttattcat cactcgcagt aattttaatc tgagttgagg ttaaaaaaca
     2521 atgagcaccg ataaacaagt ttacccac
//
LOCUS       DAZRUF010000355         2482 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZRUF010000355 DAZRUF010000000
VERSION     DAZRUF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.358"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            336..479
                     /locus_tag="ACHXOL_004145"
     CDS             336..479
                     /locus_tag="ACHXOL_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR6810960.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            492..701
                     /gene="cspE"
                     /locus_tag="ACHXOL_004146"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             492..701
                     /gene="cspE"
                     /locus_tag="ACHXOL_004146"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR6810961.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            867..1676
                     /gene="rlmA"
                     /locus_tag="ACHXOL_004147"
     CDS             867..1676
                     /gene="rlmA"
                     /locus_tag="ACHXOL_004147"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR6810962.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1673..2239)
                     /gene="mntP"
                     /locus_tag="ACHXOL_004148"
     CDS             complement(1673..2239)
                     /gene="mntP"
                     /locus_tag="ACHXOL_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR6810963.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          658 a          589 c          604 g          631 t
ORIGIN      
        1 cgactgtttt atgagaaaaa gtgatataac tttttattca ttgcatagca aaaaatgtga
       61 tattgcacgc actatgtaat aacttctccc actggcctgg aacaactgaa cttattgaac
      121 tatgttagaa aatacgccag tttaagtatc tgcctgaact ggcaaggtta agcacaatga
      181 tatatcggcg cgtattccgt tgcataagtg tgcaaaaaaa gtggaagacg tatcgagatt
      241 tgtgcgtctg atcgagacat gtttaaaaat ggcttgccat aattaacgtt gtatgtgata
      301 acagatttcg ggttaaacga ggtacagttc tgtttatgtg tggcattttc agtaaagaag
      361 tcctgagtaa acacgttgac gttgaatacc gcttctctgc cgagccttat attggtgcct
      421 catgcagtaa tgtgtcagtt ttatctatgt tatgcctgcg ggcgaagaaa acaatctaag
      481 gaatttttca aatggcaaag attaaaggtc aggttaagtg gttcaacgag tctaaaggtt
      541 ttggcttcat tactccggct gatggcagca aagacgtgtt cgtacacttc tccgctatcc
      601 agggtaatgg cttcaaaact ctggctgaag gtcagaacgt tgagttcgaa attcaggacg
      661 gccagaaagg tccggcagct gttaacgtaa cagctatctg atcgaatcca ctgatctgaa
      721 gtgtgaatac gcttcaatct cgctataaag cctcgtcgaa tgcgaggctt tttactatgc
      781 tttatcttcg ctcctggcgt tcggatattt gcccgccgcg tgattcgcgt tacacttgcg
      841 gcctttagta tcctgccgga gttgtcatgt ctttttcctg tccactttgc catcagcctc
      901 tttcgcgtga aaaaaacagc tatatctgtc cccagcgaca tcagtttgat atggcgaaag
      961 aagggtatgt caatctgctg tccgttcagc ataaacggtc tcgtgatccg ggcgacagcg
     1021 cggaaatgat gcaagcacgc cgcgcattct tagatgccgg acattatcag ccgctgcgtg
     1081 atgcaattgt cgcccaactg agggaacggc ttgatgataa ggccacggcg gtgctggata
     1141 ttggctgtgg tgaagggtat tacacacacg catttgccga tgcgttgccc gaaatcacca
     1201 cgtttggtct ggatgtttcg aaggtagcga taaaagcggc ggcgaaacgc tatccgcagg
     1261 tcactttttg tgtcgcttcc agccaccgtt tgccgttttc cgataccagt atggacgcca
     1321 taatacgtat ttacgcgccg tgtaaagcag aagaattagc acgagtagtg aagcccggcg
     1381 gctgggtcat tactgccacg ccgggaccgc gacatttgat ggagctgaag gggctgattt
     1441 acaatgaagt acatcttcat gcacctcatg cagaacaact ggaaggtttt acattacagc
     1501 agagtgcgga gttgtgttat ccgatgcgtc ttcgcggtga tgaagccgtc gcattattgc
     1561 agatgacgcc gtttacctgg cgtgcgaagc cagaagtctg gcaaacactg gcagcaaaag
     1621 aagtgttcga ctgccagacg gactttaata ttcacctctg gcagcgttct tattaaccgt
     1681 ggaagtgcgt ccagaggatc tggacgccga tgccgatcag taccagcccg ccgagaattt
     1741 ccgctttttt cccaataatt gagccgataa agcgaccaac catcatccct aatgttgaca
     1801 taatcaaggt tgcacaacca atggccaatg cggtcgcgat aatgttgacc tgcaggaaag
     1861 caagaccaac acccacagcc atggcatcca ggctggtggc aatcgcggtg gttaccagta
     1921 gccagaaacc gtgtcgacgg cgcggctctt catcttcatc atctgcgcca cgaaaaccct
     1981 caataatcat tcgcccgccg aggaatatca gcagcacaaa cgcaatccag tggttccatt
     2041 caaggacaaa ccggctggct aacatgccca ttccccagcc gatcagcggc gtcagggttt
     2101 cgacggcacc aaaaataagg ccggttcgca atgcttcaga aaatttcggt ttatggaggg
     2161 tggcaccttt accgattgat gcagcaaatg catccatcga cataccaaac gcaagaagaa
     2221 cagtagcagt gatattcatg acaatgtcct gaccggggta tccataaaac acatcatcgc
     2281 ccccagcaaa catgcataca tgaacaacaa gccgtgatgg ttttcgcgtt catgcaggca
     2341 tagcgattga tgtgcatatg gtctcgcctg actgaaagta ttcagtccgt acgtgccacg
     2401 ttttgcaacg agtatgttga cacgtacatt tccggaatct ggaaatcggc tactccccaa
     2461 atgagggcgc aaccttaaca ta
//
LOCUS       DAZRUF010000356         2448 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZRUF010000356 DAZRUF010000000
VERSION     DAZRUF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.359"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            70..1002
                     /locus_tag="ACHXOL_004149"
     CDS             70..1002
                     /locus_tag="ACHXOL_004149"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR6810964.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(1043..2446)
                     /locus_tag="ACHXOL_004150"
     CDS             complement(1043..2446)
                     /locus_tag="ACHXOL_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFR6810965.1"
                     /translation="MVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKKKGEHAYDMT
                     LSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPITMLMHGNVI
                     PALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAPAEYGKFSFG
                     EGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLEEKKFPVGES
                     ESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLTKGNQQLGSG
                     EWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAALFKEYLPLLQ
                     KSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLNFDVKLPLNVVTET
                     AKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRYTPGKVVFNG
                     QEMSEEEFMSRAGRFVH"
BASE COUNT          634 a          545 c          510 g          759 t
ORIGIN      
        1 aaggatcatc cgttatttgg gtcgttttct gctaaaatct gcgccttgcg cttctttagc
       61 gcactgtcta tggctaacct tttgaataaa ttcattatga cgagaatact cgctgcgata
      121 acccttttgc tgagtatcgt attgaccatt ttggtcacta ttttttgttc cgtcccgatc
      181 atcattgccg ggattgtaaa actattgcta cctgtgccag tcatttggcg aaaggtttcg
      241 cgtttttgtg attttatgat gtattgctgg tgtgaaggtc tggcggtatt actgcatctg
      301 aacccacacc tgcaatggga agttcacggg ctggaagggt taagtaagaa gaactggtat
      361 ctgcttattt gtaaccaccg tagttgggca gatattgtcg tactgtgcgt gctgtttcgt
      421 aagcacattc cgatgaataa atatttcctc aagcaacagc tggcctgggt gccatttctt
      481 ggcctggcgt gctgggcgct ggatatgccg tttatgaagc gttattcccg cgcttattta
      541 ttacgtcatc cggagcgacg tggtaaagat gttgaaacca ctcggcgttc ttgtgaaaag
      601 tttcgcctgc atcccaccac cattgtcaat ttcgttgaag gctcccgttt cacgcaagaa
      661 aaacatcagc aaacccactc cacttttcaa aacttgttgc caccaaaggc ggcaggcatt
      721 gcgatggcgc taaatgtact gggtaaacaa ttcgataaac tgcttaatgt tacgctgtgt
      781 tatccggaca ataaccgtca gccgttcttc gatatgttaa gcggtaaact gacgcggatt
      841 gtcgtacatg tggatttaca gcctattgcc gatgagttac atggcgacta catcaatgac
      901 aaaagcttta agcgccattt tcagcaatgg ctgaactcat tgtggcagga aaaagaccgg
      961 cttctgacgt ctctcatgtc atcacagcgt cagaataagt aagttttact cgcctctgaa
     1021 tgagcagagg cgagtgagta ttttaatgaa caaaacgtcc ggcacgagac ataaattctt
     1081 cttcgctcat ctcctgtccg ttaaacacaa ctttacccgg tgtataacgc agctgcagcg
     1141 aggcggtatt gttgtcaatc gtgattaact gaaacatctg acccaatgtc atcatcccgc
     1201 tgatttgttt atcagcctgc ttttgcgctt tttccgcatc cattccttca gataaattaa
     1261 gctgttttgc ggtttctgtg acgacattaa gcggtaactt cacatcaaaa ttgagcaatt
     1321 tgatatcttt gtttgtggat gatgaagatt tggctgggtc ggcaatactg atatccagat
     1381 tggcatttag ttcgccgagt gcgttcttcc atcttaccgg ttgtttaatg gtcggctcac
     1441 ttttttgtaa taacggtaaa tactctttga acaatgcagc attcacttct tgcagagcaa
     1501 cttcatcgtt tgctaactca gggtgtacag caagctgctt ctgcatagca atgttatact
     1561 ggatgataaa ttggcgcact gctgagggat caatagattc agcaatcaat gaccactcac
     1621 cactaccgag ttgctgattc ccttttgtta acttatcaag ttcgtaggtc agattgacat
     1681 tgatataatc tttgtcttta acgcgatcca gcatggtttt tgcaacgaaa gcatcgattt
     1741 gggcaacgtc ttccccgtga ttgataatgt taatctgatt aaatttcgac tcgctttcac
     1801 caaccggaaa tttcttttct tctaatcgcg ccagagaatc aatggtaaaa ctttttgccg
     1861 ttactttgtt cattggtgat aattgcagaa caatatcttc gactttgcct tcaatatcca
     1921 ggttagacaa tgaactacca tcaccattaa aagtaaactg gccctcacca aaagaaaact
     1981 ttccatactc tgcaggggca acatctaatg tcgtagagaa tgagccacca aacgcgaatc
     2041 gcaatgtcgc ttccacgggc gatttatttt tcgcggcgat aaatagcggt tgtgtcagtt
     2101 cattattcac taagttcact tttgccgctg ccagtgctgg gatgacatta ccatgcatta
     2161 acattgtgat gggcaacgga ccgtgctcaa catccacatc aaatacaact ttctggcctg
     2221 gcttgatatt gagatcgggt gcaccgttat cgaatgtcat ttgcatttga aaacgagaat
     2281 taaaaaaacc tttgtcaaaa tttttataac ttaacgtcat atcgtaggca tgttcacctt
     2341 tctttttagc atcgttaaaa tctttaataa acttttcgac accaggctga atttgcgtac
     2401 ctgtgtacca tgtgccacca ccccagatta cggctaacgc aacaataa
//
LOCUS       DAZRUF010000357         2448 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZRUF010000357 DAZRUF010000000
VERSION     DAZRUF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.360"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(27..317)
                     /locus_tag="ACHXOL_004151"
     CDS             complement(27..317)
                     /locus_tag="ACHXOL_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR6810966.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(307..744)
                     /gene="ratA"
                     /locus_tag="ACHXOL_004152"
     CDS             complement(307..744)
                     /gene="ratA"
                     /locus_tag="ACHXOL_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR6810967.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            915..1397
                     /gene="smpB"
                     /locus_tag="ACHXOL_004153"
     CDS             915..1397
                     /gene="smpB"
                     /locus_tag="ACHXOL_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR6810968.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1612..1974
                     /gene="ssrA"
                     /locus_tag="ACHXOL_004154"
     tmRNA           1612..1974
                     /gene="ssrA"
                     /locus_tag="ACHXOL_004154"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2173..>2448)
                     /locus_tag="ACHXOL_004155"
     CDS             complement(2173..>2448)
                     /locus_tag="ACHXOL_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR6810969.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          667 a          656 c          549 g          576 t
ORIGIN      
        1 ctcaatgagc accttttttc tgtctgttat ttattcgctg atttttctgc tcgttgcctg
       61 cgaagctctt tcggatcggc aatgagaggg cgataaatct ccacccgatc gccatcatgc
      121 acactatcgc ttagttttgc cggacggctg taaatgccga ctttgttttt agttaaatcg
      181 atatcggtac gcaattccag caagccactg gcgcgaatag cttcttcaac cgtcgcgccc
      241 tcctgcagcg tcacgcgctg caggtactgc ttctcaggta gcgcataagc cacctcaacg
      301 gcaattttac ctagcactgt aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt
      361 agacgccagc tctttaaaca cgcgaccaaa ggcgagttca atcaacttat tggtaaactc
      421 aaagtcgaga tgaaactcga tacgacacgc ctcctggctc agcggcgtaa acttccatcc
      481 accaatcaat ttcttgaacg gcccatccac cagattcata agaatacttt ggttactggt
      541 caactggttg cgggtagtaa acgttttgct gatcccagcc ttagagacat ctaccgccgc
      601 agtcatctgc ccaggagtgg actccagaat ccgacttccg gtacaacccg gcaaaaactg
      661 aggataagac tgaacgtcat tcactaactg atacatttgc tccgcgctgt agggtaccag
      721 tgcggtccgg ctaatctgag gcataactat ttccatcaac aaaaatccaa caaataatat
      781 catttatcct gctgtaaaaa aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta
      841 agatagagcc ttgtcccccg caggattgat atggggtgtt ttcgatttca gattaccgat
      901 gattcacgac gcttatgacg aagaaaaaag cacataaacc tggttcagcg accatcgcgc
      961 ttaacaagcg cgcccgtcac gaatacttta tcgaagaaga gttcgaagcg ggacttgccc
     1021 tgcaaggctg ggaagttaaa tccctgcgcg caggaaaagc caatatcagc gacagctacg
     1081 tccttctgcg tgacggagag gcatttctgt ttggcgctaa catcacgcca atggccgtgg
     1141 cctccacgca tgtggtgtgc gatcctaccc gtacccgcaa gttacttctc aaccagcgcg
     1201 aactggactc attgtacggt cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc
     1261 tgtactggaa aaatgcctgg tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc
     1321 acgataaacg ttcagatatc aaagaacgcg aatggcaggt ggataaagca cgtatcatga
     1381 aaaacgccca ccgttaaacc tgcactccaa ttattgacca gttcctcacc gcgcctccct
     1441 ctccggcggc gcgaatgaac atcttattgg ctatcacatc cgacacaaat gttgccatcc
     1501 cattgcttaa tcgaataaaa atcaggctac atgggtgcta aatctttaac gataacgcca
     1561 ttgaggctgg tcatggcgct cataaatctg gtatacttac ctttacacat tggggctgat
     1621 tctggattcg acgggatttg cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta
     1681 aaaagccgca aaaaatagtc gcaaacgacg aaaactacgc tttagcagct taataacctg
     1741 cttagagccc tctctcccta gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc
     1801 aaaagagatc gcgtggaagc cctgcctggg gttgaagcgt taaaacttaa tcaggctagt
     1861 ttgttagtgg cgtgtccgtc cgcagctggc aagcgaatgt aaagactgac taagcatgta
     1921 gtaccgagga tgtaggaatt tcggacgcgg gttcaactcc cgccagctcc accacttttg
     1981 acaggactgc aaccggacag agactataaa aacagtcact tacggacact gaccagacag
     2041 taagcggacc agaaaaagac aaaaatatac acgcaaaatg cacgtgcatt tcaaaaaagc
     2101 ctctgattat gaagtcagag gcttttctag ttgtaaataa gggtaacaat ttaacacgat
     2161 gcagcttacc aattacgtaa catcaaacgc tctaattgtt cccaaatcct ccagaccact
     2221 cagctcctct ttcatttcac gctgacggcg ataaatctca tcgttgcgat cgacctgcgc
     2281 ctgcaccatt gctgccgcca gttcttccag ttccggcatc gacagtttca cctgctgatt
     2341 atcggcatca ccccacgcca tatgcgtttg tgctgtgaca gattttgcca gcatgactac
     2401 cggggacagg cggcccagtg agtcggggcc agcattccag atacgacc
//
LOCUS       DAZRUF010000358         2439 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZRUF010000358 DAZRUF010000000
VERSION     DAZRUF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.361"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHXOL_004156"
     CDS             complement(<1..458)
                     /locus_tag="ACHXOL_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR6810970.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTSDTALLKSFEAFREWVAIQAGFYTEHFYPDGSRGCRAKSIA"
     gene            complement(497..784)
                     /locus_tag="ACHXOL_004157"
     CDS             complement(497..784)
                     /locus_tag="ACHXOL_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810971.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(1034..1189)
                     /gene="hokD"
                     /locus_tag="ACHXOL_004158"
     CDS             complement(1034..1189)
                     /gene="hokD"
                     /locus_tag="ACHXOL_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR6810972.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1417..2082)
                     /locus_tag="ACHXOL_004159"
     CDS             complement(1417..2082)
                     /locus_tag="ACHXOL_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR6810973.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2251..>2439)
                     /locus_tag="ACHXOL_004160"
     CDS             complement(2251..>2439)
                     /locus_tag="ACHXOL_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810974.1"
                     /translation="CGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQ
                     IERNAKARIRRATKSRTE"
BASE COUNT          608 a          640 c          543 g          648 t
ORIGIN      
        1 gcgatagatt tcgcccgaca cccacggcta ccgtccggat aaaaatgctc ggtgtaaaat
       61 ccggcctgaa tggctaccca ctcgcggaaa gcctcaaacg actttagcaa tgccgtatca
      121 ctggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc ggcctactga ttcacacagg taatcaacga aaccggacac cagttttcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaaacccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cacacacgct taaagtctgc gtgtatccac
      361 tcacctattt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgac cagttgtata tgtgccatcg gctttctccg gtggcacggt
      481 gttacacagc aggagttcaa tcctgctcaa gattgtagat gagtttattc ttcagcaaaa
      541 gcagaaaacc agccttaaaa ccaatctctt tcaaaacccg taatgatgtg acaaattcgt
      601 cctcacgcaa aataaaaccg tccgtcagaa gtccattaca aaaataaaat aacacagcac
      661 cgctcttcct ttgttgagat tgcaaacatc taatgcggca atggctgaca atcgctccat
      721 tctcaacgcg cacagcatag aggccatttt cactaaaaat ttcacgcaat tcttcgattt
      781 tcatcttcag aatccttcca gataaatagc tctcccctgt tcggggtcca tccctcttct
      841 ccctgcgcgc tacttaagtg agtcgattct atctgcgaag gtgcgcgaat caaattcacc
      901 ggaaataaac aacaaaaaac ccgccgaaac gctgagagat cacagcgatg ggcgggttaa
      961 gtgcgggtgc gttgaggatg cctgacacat cagaggtggg cggggatggg atcagctccc
     1021 cgcccggtca ctcttacttc ctggattcgt agtctacgaa gacagcgacc tccgtctggc
     1081 cggttcggat tcgtacctca cagaggtctt tcctcgttac cagtgccgtc actatgacgg
     1141 ttaaacagat gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct
     1201 ccttgccttt cggcatgtaa gaggctaacc tacatgtgca aagcatgaaa ttggcctcag
     1261 attaatgtta agcgtcttgc cggacgcgta atgttaactg gggcttttct ctatctgcct
     1321 tttggtgttc atgcctgaag cagatagcct caagcacccg cagtcattct acttacctcg
     1381 ccaatatgaa atcaatcaga aaggtgcccc ataaaatcac tccttctctt ctttaccgta
     1441 gtggagttga ccaattttga taagagggcg tccctgagat ttgcggtgta gattggtatc
     1501 gcgcagagaa tacacacagc cacaatattc ctgctgatag aatttttcgc gcttgctgat
     1561 ttcaatcata cgggacgagc cgccctgctt gcgccagtta taatcccagt acaccatgcc
     1621 cggataatgc gcgacggctc gccgcccaca gtcgttaacc tgctgcatat ttttccagcg
     1681 tgaaatgccc agtgaactgc tgatcacact gaaaccattt tcagcggcgt acagcgctgt
     1741 ccgctcaaaa cgcatgtcaa aacacatggt gcaacggatc cctcgttcgg gctcccattc
     1801 cattcctttg gctcgttcaa accagttgtc tgtgtcgtaa tcagcatcaa taaacggcac
     1861 gccgtgttgt tcagcaaagc gaatattctc atccttacga attaaatact ctttctgagg
     1921 atgaatgttc gggttgtaga aaaagatggt gtagtcgatt cccgaggcct gaagcgcctc
     1981 catcacctca ccggaacatg gagcacagca agagtgcagt agtagtttgt ttgccccgtt
     2041 tgggagctcc aatttgggcc gtttgaaatc agcaactgtc ataaatatgt ttattggggt
     2101 cataaaaata tcaaagagtg tagcattaga acggggctat cggaaacaga tgtgtgactg
     2161 ctccccgccc tttcgggtgg tctcctgatg atttgagggt gcagaaatcc ctccggttaa
     2221 ggattaaatt tttaacagtg ctaaatttaa ttattcagtt ctggattttg tcgccctgcg
     2281 tatccgcgct ttcgcgttac gctcaatctg tatcagcttt tctatatttc tccgcctttc
     2341 ctgttcctcc tggcgcaata gccttacatc atctgccagt ctggtttctc tttttgccac
     2401 agagagcatc cagtcaaacg gctccacaac cgcaccgca
//
LOCUS       DAZRUF010000359         2375 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZRUF010000359 DAZRUF010000000
VERSION     DAZRUF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.362"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..936
                     /locus_tag="ACHXOL_004161"
     CDS             <1..936
                     /locus_tag="ACHXOL_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR6810975.1"
                     /translation="SMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEEDLHIQLF
                     ERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPTAVFYFL
                     PLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFTPLVNEP
                     FVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLDWFYEVR
                     HLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKDAVLSPA
                     AERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(977..1972)
                     /gene="pgl"
                     /locus_tag="ACHXOL_004162"
     CDS             complement(977..1972)
                     /gene="pgl"
                     /locus_tag="ACHXOL_004162"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR6810976.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2268..2363)
                     /locus_tag="ACHXOL_004163"
                     /pseudo
     CDS             complement(2268..2363)
                     /locus_tag="ACHXOL_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
BASE COUNT          641 a          599 c          550 g          585 t
ORIGIN      
        1 agtatgaagg catttgtcat actggcagag tccagttcgt ttaacaatgc cgctaaatta
       61 ctcaatatta cgcaacctgc attaacgcga agaataaaaa agatggagga agatttacat
      121 attcaactat ttgagcgtac aactcgcaag gttacgttaa caaaagcagg aaaaaggttg
      181 ctcccggagg cgcgggaatt aataaagaaa ttcgatgaaa cgctttttaa tattcgtgat
      241 atgaatgctt atcatcgtgg tatggtgacg ttagcgtgta ttccgaccgc agtattttat
      301 tttttaccgc tggcaatcgg taaatttaat gagctgtatc ccaatattaa agtgcggatt
      361 ctggaacaag gcacgaataa ttgcatggaa tcggtgctgt gtaacgaatc agactttggc
      421 ataaacatga acaacgtcac aaattcatcc attgatttta ccccactggt caacgagccg
      481 ttcgtgctgg cctgtcggcg tgaccatcca ttggccaaaa agcagcttgt agaatggcag
      541 gagttagtgg gttacaaaat gattggcgta cgctcctcca gcggcaaccg actgttgata
      601 gaacaacagc tggccgacaa gccctggaag ctggactggt tttacgaagt gcgtcatctt
      661 tcgacgtcat taggactggt agaggcggga ctggggattt cggcgcttcc tggtctggca
      721 atgccccatg cgccctattc ttcgattatt ggtattccac tggtggaacc cgttatacgt
      781 cggacattgg ggattattcg ccgcaaagat gccgtgcttt ctccggcagc agaacgcttt
      841 tttgccttgc tgattaattt gtggactgac gataaagaca atttgtggac caatatcgtc
      901 gaacgccaga gacatgcgtt acaggagata ggttaataca ccggcgcagg agagactgcg
      961 ccgggtaaat cagcggttag tgtgcgttaa ccaccaccca cattggtccc tgcccgaccg
     1021 catagcggcc tttttcatgc agtagcccct gctcgccaac aatttcgtat accgagatgt
     1081 ggtgagattt ttgcccggcg gcaatcagat acttgccgct gtgatcaaca ttgaagccgc
     1141 gcggctgggt ttccgttggc tggaagcctt ctttactcaa cacgctgcca tcttccgaaa
     1201 cgctgaaaac ggtaatcaga ctggcggtac ggtcgcaggc gtataaatga cgaccatccg
     1261 gggtgatatg aatatcagcc gcccaacagg tgtcggagaa gttttccggc atcatatcca
     1321 gcgtctggac acattcaata ttgccgtgtg gatctttcag ttcccagaca tccactgagc
     1381 tgtttaactc attgacgcaa tacgcatatt gttcgtttgg atggaatacc atatgacgcg
     1441 ggccggcccc ttcaacggta gtcacttccg cagggtcctg cgccaccaga tgaccatcat
     1501 cgctgaccgt aaacaggcaa atgcgatcct gctttaatgc cggaacccac agcgtacggt
     1561 tgtccggtga gatattggcg gaatggcaac cgtccagccc ctcgaccaca tcgacgacgc
     1621 ccactggcag gccatcttcc agacgcgtta cgctcacgtt acccgcattg taagaaccta
     1681 caaagacaaa ctgcccctgg tgatcggtgg aaatatgcgt cggactaccc ggcagcgcag
     1741 actctgcggc aaaggtcagt gcgccatcgt ccggggcaat acgatacgcc aggacgcgaa
     1801 actcagggcg aacaccaaca tagagataac gtttgtccgg gctgaccacc atcggctgca
     1861 cctgccccgg cacatcgaca acctgtgtca gcgtcagtgc gccttcatga ttcaggttcc
     1921 agacgtgaat ttgctggctc tcagggctgg cgatataaac tgtttgcttc atgaacacac
     1981 ctcaaaaatg ataaatccct tatctttcat acacataaca acacttactg gataaaaaac
     2041 agacaacaaa acacgataaa atacaatatc gcacatcaat taaatcaata agttagcact
     2101 ccatttcgaa tggcatatta ctaaaatatt agtcaccgac acgaatttga caatgccaca
     2161 gatattttcg cgactattca gggagtatga ctctccccgc ttttgtttac ctgtatcaac
     2221 gaccgtaaac actatgccat aacaacttct ggccaacttc ccattcgctc aacaatcaac
     2281 tcacttccgg cacaaataaa cgcattacgt tgtagatcgg tattctggtc atttgttgat
     2341 acgcgtacat agccaataag catggtaaat ccccc
//
LOCUS       DAZRUF010000360         2312 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZRUF010000360 DAZRUF010000000
VERSION     DAZRUF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.363"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            37..729
                     /locus_tag="ACHXOL_004164"
     CDS             37..729
                     /locus_tag="ACHXOL_004164"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR6810977.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPRPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(778..2277)
                     /gene="pitB"
                     /locus_tag="ACHXOL_004165"
     CDS             complement(778..2277)
                     /gene="pitB"
                     /locus_tag="ACHXOL_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR6810978.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          616 a          591 c          562 g          543 t
ORIGIN      
        1 tatttttaat ggatatttaa attaaaggat atattcatgc agtcaataac acctccatta
       61 attgccgtta ttggtagcga tggttcaggc aagtcaacgg tgtgtgaaca tcttattacc
      121 gttgtcgaaa aatatggtgc tgccgaaaga gttcatttag gaaaacaggc cggaaatgtc
      181 ggtcgtgcag tgacaaaatt accgttgatg ggaaaatcct tacataaaac aattgaacga
      241 aatcaggtga aaacagcaaa aaaattgcct agaccagttc cggcgctggt aattacagcg
      301 tttgtcgccc gtcgcttact gcgctttcgt catatgcttg cctgtcgtcg tcgcgggtta
      361 attgttctaa ccgaccgtta tcctcaggac caaattcctg gcgcttacga tggtacggtg
      421 ttcccaccta acgttgaagg tggtcgtttt gtctcatggc tggcaagcca ggaacgtaaa
      481 gcgtttcact ggatggcgag ccataagcct gatctggtca tcaaactcaa tgttgacctt
      541 gaagttgcct gtgcacgtaa acccgaccat aaacgggaat cgctggcgag gaagattgcc
      601 ataacgccac agttaacctt tggtggtgca caactggttg atatcgatgc caatcagcca
      661 ctggaacagg tgttggttga tgcagaaaaa gcgattacgg attttatgac cgcgcgtggt
      721 tatcactagt caaaaatgga aatgcccgat cgccaggacc gggcattttc aggaaggtta
      781 aatcaactgc aatgctatcc agtacagccc accagaaaga aaaattgccg ccggtaaagt
      841 aaacacccac gccatcagga tgctggttac cgttttacgc tgtaacccac cgccgtccac
      901 caccatcgtc cctgctaccg cagacgagag gacgtgtgtt gtggagacag gcatcccaat
      961 ataactggca aggccgatag acactgccgc cgtcatttgt gccgccatgc cttgcgcata
     1021 cgtcatgccg cgcttaccaa tcttctcacc gatggtcatc gctacacgac gccagccaat
     1081 catggtgcca atgccgagcg ccagtgctac cgccatgatg atccacacag gagcgtactc
     1141 aatggtgctt aacatatcgc tgcgcagttt tttcagcagg ttctggtctt ctttactgac
     1201 gcctggcagt ttcgccagct tcgcggaggt atcagagatg cacagcataa tgcggcgcag
     1261 ctggctgcgc tgactcacgc ttaacggctc gtaactttcc atattgcctg gcagcatcgt
     1321 tttaacgcgc gcaatcgcat caaaggtatt tgccggatga cagtgaaact ctgttacttg
     1381 cgtgccatca gtcgatgctg caggcaatgg aggttccatc gcaatcaact tctgcggcag
     1441 ttcaggatgc tgttgcaggt agtgttcgaa gttggtgacg gcatcgcggg tacgggtaat
     1501 ttcatagccg gacgcgttca tattgacgac gaagccagca ggggcaatcc ccaccagtac
     1561 cagcatcacc aggccgatcc ctttttgtcc gtcgttcgcg ccgtgcgaaa acgccacgcc
     1621 cgcagcggaa acaatcagcg caatacgcgt ccagaatggc ggtttacgtt tgcctttttt
     1681 ctttttgcga tcttccggaa tgcggtgaat acggtcacgc tttttcgtcc cgctccagta
     1741 gcgtcgcagc aggaatatca ggcctcccgc aatgaccagg ccgacgatag gggaaacaat
     1801 cagcgaggag aaaattttgg tcacttcacg caggtttaac gcatccatca ccgatgatcc
     1861 ggttaacagc gcgttggtta aaccgatgcc gataatcgca ccaatcaagg tgtgcgaact
     1921 ggaggccggt aaaccgaaga accacgttcc caggttccag ataatcgccg ccagcagcat
     1981 ggaaaagacc atcgccaggc cgtgggttga ccccatattc agcaacaaat cggttggcaa
     2041 catatggaca atggcatagg caacgctaag tccgcccaat aacacgccaa aaaagttaaa
     2101 aaatgccgcc atcaccacag caagttgtgg ttgcatggca cgagtataaa taacggttgc
     2161 caccgcattc gccgtgtcat gaaaaccatt gattgcttcg tagaacaaca caaatgccag
     2221 agcaagcaat aacaaaagcc ctgtgtatat atcaaggcca acaaataaat ttagcatata
     2281 ttagattacg ccattttgaa tttacgaacg ga
//
LOCUS       DAZRUF010000361         2270 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZRUF010000361 DAZRUF010000000
VERSION     DAZRUF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.364"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..177
                     /locus_tag="ACHXOL_004166"
     CDS             <1..177
                     /locus_tag="ACHXOL_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR6810979.1"
                     /translation="TTEFLFLILFIVLMTPISPLIFVWIIGKIIEPVIELYNDVVWAS
                     FNTLHNKINPYKES"
     gene            179..544
                     /locus_tag="ACHXOL_004167"
     CDS             179..544
                     /locus_tag="ACHXOL_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR6810980.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            541..1206
                     /locus_tag="ACHXOL_004168"
     CDS             541..1206
                     /locus_tag="ACHXOL_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6810981.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1206..1571
                     /locus_tag="ACHXOL_004169"
     CDS             1206..1571
                     /locus_tag="ACHXOL_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR6810982.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2026..2181
                     /locus_tag="ACHXOL_004170"
     CDS             2026..2181
                     /locus_tag="ACHXOL_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR6810983.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          602 a          488 c          613 g          567 t
ORIGIN      
        1 acaacagaat tcctgtttct cattctgttt atcgtgttga tgacaccgat atccccgcta
       61 atttttgtct ggataatcgg aaaaataatt gagccagtta ttgaattgta taacgacgtg
      121 gtatgggcgt cattcaacac actgcacaat aaaattaatc cgtataagga aagctgatat
      181 ggcactgacg aaaaaacaac gtgcagaact gcgcatgaag ttcggtggtc gctgcgctta
      241 ttgcggctgc gaacttggcg aaaagtggca tgcagaccat gtaaaaccgg tcattcgttt
      301 tgatggaaat atgcttcacc aggaacgtga cgatatatcc aacatggtac cagcatgcca
      361 cccatgcaat ctgcacaagc attgcagtag cctggaagat tatcgacgaa ttatcagtga
      421 tggtcgtcgt gaattccttg cgtccgggaa aggcaaagcg ctggttcgta tgggattggt
      481 tgaaatgaaa tctgacccgg ttgtgttctg gtttgaaaaa tatcaagaag gggctacggc
      541 atgaccacta ttaccaaaga gcgactgcaa tggctggcta acatttctgg ccgcgatgat
      601 attgacgata tagacggcgg tgaaattcgt gaactggcgc ttatcgctct ggcatcactg
      661 gaagcagagc ctgtagcgga atgcattgtt gaagatgggg gcatgtgtat tgacgggttc
      721 ggtgagtatg tgggtcactc gctgcctgat ggaatgcatg agctttatgc tgccccgcga
      781 atgcgacaac ctgatggatg gaaagccgta gctgtagcgt ggaaggtgac gtttactcaa
      841 gttgaccagg aatctaatac gttcactgct atatattttg acaaagcgga agttgagtgt
      901 tgggtacgac tgcataaagc atgtgatttt cgggcagata taacaccgct ttacgcagcg
      961 ccggcagtgc cggttgcagt aaacgacgac atggcttacg cattccatca cgcactgtca
     1021 gattcatcgc ttggctctga tgaaatcgaa gaaattaaaa ccggtttgcg tgctgccttt
     1081 gccaacgtca ctatccaacc agagccgata gtgccggatg aaatcgggcc aaacgatagt
     1141 aatacgtttg attatgttga tggctggaac gcctgccgcg ctgccatgct taagggagat
     1201 aaataatgat taatcgaacc aaactggagc acattctcga atatgccagg cagcagaaat
     1261 gtattgggca actttgtaaa attccaccag gagatatggt tgaaatcgtg gaaatggcca
     1321 tgcgtaaggc tggcaactct ccggtagctc cggctggctg gataagctgt agtgatgcag
     1381 ttcctgctga atattgcgat gtgattcttc tcgatgatct cgggaatgta ttccccggtt
     1441 cctgggataa ggttttttgc cccactcgtg gcgggaataa gatggctttt gtggacaaag
     1501 acggcgttga agtagagagc tcaactcact ggatgccgct accggaacca ccgcaggagg
     1561 tgaatcaatg acctggcctg aggcattcac cacggtagga attgcgatgg cggtggcgct
     1621 ggtggtgtat tcgatttgcc gttggggtta acaaacaaaa accccggatt gatggtccgg
     1681 ggtttttgaa ggaaacaaaa cagaaacagc aattgccgtt acctgttgtt accatggcaa
     1741 gtaaacgtat ctcaggcgag cgcattgcgc cgttctgacg cagataaact agcctggata
     1801 gatggtgctg gcaataaaaa atagcgtttt cttatcggtg tcggtaagat tgctgcgggt
     1861 gcttgaggct gtctgcctcg ggtatgtcac tgtaaggcag acagagaaaa gccccagtta
     1921 acattacgcg tcctgcaaga cgcttaacat taatctgagg cccaatctat gtctcacaaa
     1981 tgtaggttag cctcttacgt gccgaaaggc aaggagaagc aggctatgaa gcagcaaaag
     2041 gcgatgttaa tcgccctgat cgtcatctgt ttaaccgtca ttgtgacggc actggtaacg
     2101 aggaaagacc tctgcgaggt acgaatccga atcggccaga cggaggtcgc tgttttcgta
     2161 gactacgaat ctagagagta agagtgacca ggcgggagag taatctcccg ccacctttga
     2221 tgtgtcaggc atcctcaacg cacccgcact taacccgttt cggcggtttt
//
LOCUS       DAZRUF010000362         2238 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZRUF010000362 DAZRUF010000000
VERSION     DAZRUF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.365"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            126..458
                     /gene="hdeA"
                     /locus_tag="ACHXOL_004171"
     CDS             126..458
                     /gene="hdeA"
                     /locus_tag="ACHXOL_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR6810984.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            574..900
                     /gene="hdeB"
                     /locus_tag="ACHXOL_004172"
     CDS             574..900
                     /gene="hdeB"
                     /locus_tag="ACHXOL_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR6810985.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            964..1611
                     /locus_tag="ACHXOL_004173"
     CDS             964..1611
                     /locus_tag="ACHXOL_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR6810986.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1653..2183)
                     /locus_tag="ACHXOL_004174"
     CDS             complement(1653..2183)
                     /locus_tag="ACHXOL_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR6810987.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT          672 a          427 c          496 g          643 t
ORIGIN      
        1 ttgaaataaa aatatctgat tttgatattt tatatcaaca tgacatatac agaaaaccag
       61 gttataacct cagtgtcgaa attgattcgt gacggctctt tcactttata gttgaggata
      121 ttacgatgaa aaaagtatta ggcgttattc ttggtggtct gcttcttctg ccagttgtga
      181 gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa accggtcaac tcctggacct
      241 gtgaagattt cctggctgtg gacgaatcct tccagccaac tgcagttggt tttgctgaag
      301 cgctgaacaa caaagataaa ccagaagatg cggttttaga tgttcagggt attgcaaccg
      361 taaccccagc tatcgttcag gcttgtactc aggataaaca agccaacttt aaagataaag
      421 ttaaaggcga atgggacaaa attaagaaag atatgtaatt ccgggaatgc gttacatcgt
      481 acttccttgc atattgaaca ggccggaata tcttctttaa aagcagctat tcctcctgtt
      541 catatataat ctctatattg aatgggttac aaaatgaata tttcatctct ccgtaaagcg
      601 tttattttta tgggcgctgt agcggctttg tcactggtga acgcacaatc tgcgctggca
      661 gccaatgaat ccgctaaaga tatgacctgc caggaattta ttgatctgaa tccaaaagca
      721 atgaccccgg ttgcatggtg gatgctgcat gaagaaacag tatataaagg tggcgatacc
      781 gttactttaa atgaaaccga tctcactcaa attcctaaag tgatcgaata ctgtaagaaa
      841 aacccgcaga aaaatttgta taccttcaaa aatcaagcat ctaatgactt gccgaattaa
      901 tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt gctgctcctt tttgcatttt
      961 tatatgacag cagaatttat tatacgtctt atactcgcgg caattgcctg tggcgctatt
     1021 ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac gcacacatgt attaattggc
     1081 atgggaagcg ccctgtttat gattgtttcg aaatatggtt ttgctgacgt gctgtcttta
     1141 gatcacgtcg gactcgaccc cagccgtatt gctgctcagg tggtgacggg cgtcgggttt
     1201 atcggtgcag gtaacattct cgttcgtaac caaaatattg taggtctgac gacggcagcg
     1261 gatatctggg tgaccgccgc cataggtatg gttattggca gcggtatgta cgaactgggt
     1321 atttatggtt cagtgatgac cttgttggtg ctggaagtct tccatcaatt aaccttccgc
     1381 ctgatgaaca aaaattacca tttacagctg acattagtga acggtaatat ggtatcaatg
     1441 ctcgactggt ttaagcagca aaaaataaaa actgatttag tttcgttgca ggaaaatgaa
     1501 gatcatgaag tggtcgccat tgatatccag cttcatgcca caacgtcgat agaagacctg
     1561 ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt caattagcta atctgtgtgg
     1621 cagcgtagcc agactcaccg taagcctgaa attcacacca gataatcaat atgctgatgg
     1681 cgtaccagct cattgatccg cttaagattg aggatcatca tgatattgtg tttatggcag
     1741 tagaacgttt ttagcgatat tttcagtttc tccagaatat cttcagttgt catttgattc
     1801 gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg acacattcgc ttcaggcaca
     1861 gagcagtaac tgccattgat gagtgcgaca aaatcaatga tagtgatagt ccgcggaaca
     1921 aaggtcaccg gtactttccc cagacagcgt ttaatattaa agggggagaa aacaatgaca
     1981 tggacaggtt ttaatcgttc aatttgagtg agaaaattag aatgaaatac attattcatt
     2041 aatgtatcaa taatgacgaa ggcattctga tgcaacatta cgtcgatctc ttcttcgttc
     2101 tgtatatgaa cgacattacc tttactcaga atgtttttca tcgcggtgaa gaacatcgta
     2161 tccctggtaa ttataagaaa cataaccgac tcctggtgcg gacattaact ttataatcaa
     2221 tttgccaaag gtaattaa
//
LOCUS       DAZRUF010000363         2206 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZRUF010000363 DAZRUF010000000
VERSION     DAZRUF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.366"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(355..489)
                     /locus_tag="ACHXOL_004175"
                     /pseudo
     CDS             complement(355..489)
                     /locus_tag="ACHXOL_004175"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(502..1323)
                     /locus_tag="ACHXOL_004176"
     CDS             complement(502..1323)
                     /locus_tag="ACHXOL_004176"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR6810988.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNEGILLNGKTIRKLMKELGISSLIRRKKYRAYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1312..2206
                     /locus_tag="ACHXOL_004177"
                     /pseudo
     CDS             1312..2206
                     /locus_tag="ACHXOL_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1592..1708
                     /locus_tag="ACHXOL_004177"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          557 a          495 c          529 g          625 t
ORIGIN      
        1 gcccgaggta tattctgaaa tatcattcat catgaccggc gtttatacaa acgccggtca
       61 ttcgctaaca gcttcaccgt tccttccccc atccaggttc cgcatgagcg tgttgtaatg
      121 ttcataagcg tattatcagg attgtctgga aaattcccga tactttggac tattactcct
      181 gaacaattcg aaattcagca gattaaaggt aagcgttaac ggaggtaagc gttaacggag
      241 gtaagcgtta acggaggtaa gcgtaaaccg actgccgtat gtagccatta agccagtatt
      301 ggtaacgtag gtgcccacct tttcaaatga tctgcgcccc aaatcttgga catcctatga
      361 aaaatgttta agtcgatatt gaaccggact catgccatcg agtttctctt taatgcgatg
      421 gtgattgtag tattcaatgt aatcaattat atcttgcttt agatgatgta acttattgta
      481 tttatttagg tagtaacatt cttattttaa agtactaaaa aagctttcca ttgctgcatt
      541 atctaagcag ttaccacgtc gggacatgct ttgtgttatg ccattatcac tcaaccaacg
      601 ttgccaacga ggcatctgat attgccaccc ctgatcacta tgcaacaagg gcttatcgtc
      661 tttattgagt tgttctaccg catctaatag catgctcttt accattgatg ctaatgggcg
      721 ctcagtaagg ttatatgaga ctatttctcc attatacagg tccatgattg gtgataaata
      781 gagcttcctg ccatcgacct taaactctgt gacatctgtt acccactttt gatttgggcg
      841 gtcagcataa aattgtctct tcagtaaatt attacaagtt cgcccctgtt ctccacgata
      901 ggcacggtac ttcttgcgcc tgataagtga gctgataccc agttccttca ttaactttct
      961 gatggtttta ccattaagta aaatgccctc gttacggcag gcaagtctga tgcgtcgata
     1021 accataacgc cctttatgct gatggtatat tctcaatatt acttgtttaa caagttgata
     1081 tggatctggc gcattaaggc gtttcacgtg atagtaaaat gtactacgtg gcaaatccgc
     1141 gataaccagt aattgtgata atggatgaca ttgccttaac tcattaataa tgattacttt
     1201 ttcttttgtt ccaatcgtaa tttttcctga gctaaggctt tcagcttttt tagataatga
     1261 accgccccgg gaatcctgga gactaaactc cctgagaaag aggtaaacag gatgactaaa
     1321 aatactcgtt ttcccccgaa gtccgtcaac gggcagtccg tatggttctg gaaagtcagg
     1381 gcgaatatga ctcacaatgg gcggtaattt gttccattac tccaaagatt ggctgtacac
     1441 cggagactct gcgtgtctgg gttcgccagc atgagcggga taccaggggc ggtgatggag
     1501 ggctcaccac cgctgaacgt cagcgtctga aagagctgga gcgtgagaat cgtgaactgc
     1561 gctgcagtaa cgatatcctt cgccaggctt ccgcttattt tgcgaaggcg gagttcgacc
     1621 gcctctggaa aaaataatgc cactgctgga taagctgcgt gggcagtacg gggtcggacc
     1681 ggtatgcagt gaactgcata ttgccccgtc aacgtattac cactgttagc aacaggacat
     1741 catcctgata aacgcagtgc ccgtgcgcag cacgatgact ggctgaagag agagatacag
     1801 cgcgtatacg atgaaaatca tcaggtgtac ggtgtgcgta aagtctggcg ccagttgtta
     1861 cgggaaggaa tcagggtggc cagatgtaca gtggcgcgcc tcatggcggt tatgggactt
     1921 gtcggtgttc tccggggtaa aaaggtccgc actaccgtca gccggaaaac cgttgccgca
     1981 ggtgaccgcg taaaccgtca gttcgtggca gaacgtcctg accagctgtg ggtggctgat
     2041 tttacttacg tcagcacatg gcagggcttc gtctatgtgg cgttcatcat tgatgtgttt
     2101 gccggataca tcgtgtggtg acgagtctca tcgtctatgg aaacgacatt cgtgctggat
     2161 gcactggagc aggcgttgtg ggcccgtcgt ccgtctggca ccatcc
//
LOCUS       DAZRUF010000364         2193 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZRUF010000364 DAZRUF010000000
VERSION     DAZRUF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.367"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..80)
                     /locus_tag="ACHXOL_004178"
     CDS             complement(<1..80)
                     /locus_tag="ACHXOL_004178"
                     /inference="COORDINATES: protein motif:HMM:NF024418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFR6810989.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKV"
     gene            complement(442..1446)
                     /locus_tag="ACHXOL_004179"
                     /pseudo
     CDS             complement(442..1446)
                     /locus_tag="ACHXOL_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            1431..1808
                     /locus_tag="ACHXOL_004180"
                     /pseudo
     CDS             1431..1808
                     /locus_tag="ACHXOL_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1847..>2193)
                     /locus_tag="ACHXOL_004181"
     CDS             complement(1847..>2193)
                     /locus_tag="ACHXOL_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR6810990.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT          521 a          512 c          587 g          573 t
ORIGIN      
        1 ctaactttcc gggtcagttc agaaacagca actaatccac ctgtaatatg actatatttc
       61 agtgaataaa ttttattcat acgtgaactc cgatatttct gagttgtggt tatgggatag
      121 catttaatta caacggggaa ctatcagtca tggtacgtaa ctgataaggg tataatgata
      181 atcactgtca gttatatttg tatgacagat gtaacaaaat gaaaaaaaaa gataagcaca
      241 agtgattgat tggtcagata ttgatcactt ggtgtttgta ttttgaactt tgcgatcaaa
      301 atttgatctt tgttgtttcc ggtcaatcct ttacaggttt tctgtattat ccgggggagt
      361 taagtctgtc gatgtccgac agacttttga tgatggaaac aacatgcagc gataatgtgc
      421 atgctgtttt gcaggtggaa ctcagaaggt gaactggaac tgtgcgaccg caccgtaagt
      481 gttgtccgtg ccgatcaggc ctgcaacatc aaggtgaacc acatcaaacg gtgagatccc
      541 caccccggcc gtgaatgcac tgccgtcgtt accggccaga ttctgacggt aaccggcgcg
      601 cagctgtgcc catttccagg cattaaattc tgcgccaatg gcggcaaact gacggttgct
      661 gtcggaggtg aaaccgctgg ccggggtcag atccacatca aatgcggtgg tgaacatcgc
      721 gttgtgccag gagacaccgg ccgtcacctg cgggcggatc ctgaaggttt ccgtgatacc
      781 gttcacttct tttgtctcaa tactacgggg gatcaggttc tgtgcgacca gtcccagcgt
      841 ccagttgtca tccaggtccg tactggcacc gatgtcggcg ttgatgccat ttttcgtgtt
      901 gtggtagcgg tcgcctttga aggcgctgct gtcataattt ctgaccagca cgttgtagtt
      961 gaacaggtcg acgcgctgga atttgggggg gaagcccaga gaccatttct gcccggccgt
     1021 ttccagttcc gtagccagag caatacccac atcagtgatc agtgccgcgc gggcaaaggc
     1081 ctgtgatgtc aggcggcttt tgtccacctc ctgcccggtg ctgtttgcca cctcttccag
     1141 gtaagtgagg tcagcatcag agactttacc gtcgacactg acggtgccgt gtgatttaac
     1201 catcagtgcg gcagagaggc gatcaccggg aagggccgcc acagcagaaa cgcccagctg
     1261 tgcggcggca tgtgtgttac ggaagtcccg gaggtgttct ttcaggcgtg cggcagcctc
     1321 aggaacaccg tggttacttt cgatggcgcg gtcgaaggcc ttccactcat cactgatccg
     1381 gtcggcgttg tcggtgatat tatccggatt ggagagctgc gcgccgacgg acggaagcag
     1441 gatgctgggc tcatgcgcga cgcaaaatcc acgatgtata tatcagtact aaaagcgcga
     1501 cggcggaaga agctctgaaa ctaatcggtg aactgtacgc catagagcac gatatacgcg
     1561 ggttaccggt gtctgaacgc ctggcggtca ggcaaatgca gagtagaccg ctcctgactt
     1621 ccctgtataa gctgatgcag gagaaagaac acacgttatc gaagaaatgt cgtctgggag
     1681 atgcgttccg gtatatcagg aagcactggg ctgcgttgtg caacttctgt gatgatggcc
     1741 tggctgaggt agataataat gcagcagaaa gagcgcttcg tgcagtctgt ctcgggaaga
     1801 aaactttttc gaggatcact cattccgtgt ctgactgctt cagggtttaa ttttgcttct
     1861 gctgactgaa tgatgattgg gcagcaagtg caatatccca cataaatcca gttaactcac
     1921 gagccaccgc cgtaatggct acttgtgatt ttttcccttt tgctgtcaga gttcggtaac
     1981 gagaacacaa tcgttgttga gctttccatg caatatcttt ggaatattgt gttacagagt
     2041 ccggtcggta atatataagc catgctccaa cttttgcagg tgtacgataa gaccaagcgg
     2101 cttcgtaaag cagacgtctc agttcgctgt ttccaacttt tgttattcct ctgggacgaa
     2161 tactgtttcc actggaatat tcacctggca cca
//
LOCUS       DAZRUF010000365         2190 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZRUF010000365 DAZRUF010000000
VERSION     DAZRUF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.368"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            16..969
                     /locus_tag="ACHXOL_004182"
     CDS             16..969
                     /locus_tag="ACHXOL_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR6810991.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1178..2143
                     /gene="lipA"
                     /locus_tag="ACHXOL_004183"
     CDS             1178..2143
                     /gene="lipA"
                     /locus_tag="ACHXOL_004183"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR6810992.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT          582 a          545 c          503 g          560 t
ORIGIN      
        1 aattggagta agtgcgtgga tagtaataat caaattgaac cctgtttaag cagaaagtcg
       61 tcagaaggta aaccacaaat atttacaacc ttacgaaata ttgatcttaa ccttctgact
      121 atttttgaag ctgtatatgt acataaaggg atcgttaatg cagcgaaagt gcttaatctg
      181 accccctcgg caatcagtca gtctattcag aaactgcgcg ttatattccc tgacccattg
      241 tttattcgca aaggccaggg tgtcactcct accgcatttg cgatgcatct acatgagtat
      301 atcagtcagg gccttgagtc cattcttggc gcgctggata tcgaaggaag ctatgataag
      361 caacgaacga taactattgc gaccactccc tcggtcggag ccctggtcct tcctgtcatc
      421 tatcgggcga ttaaaactca ctatccgcag cttttactgc gcaacccgcc catcagcgac
      481 gcggaaaacc aactcagtca gtttcaaacc gatctcatca tcgataacat gttttgcacc
      541 aatcgtacgg tgcaacatca tgttctgttc accgacaata tggtgttaat ttgccgcgag
      601 ggaaatccac tactctcttt agaagatgac agagagacta tcgacaacgc tgcgcatgta
      661 ctcctgttac cggaagggca aaatttcagc ggtctgcggc agagagttca agagatgttt
      721 ccggaccggc aaatcaattt caccagctac aacattttga caatcgctgc actggttgcc
      781 aacagtgaca tgttagcgat tattcccagc cgtttttata acctgtttag tcgttgctgg
      841 ccgctggaaa aactgccttt tccgtcctta aatgaggagc aaatagattt ctctatccac
      901 tacaacaaat tcagcctgcg tgatcccatc ctgcacgggg taatagatgt tatccgtaat
      961 gcattttgaa aaagtattgt tacagccaga tcacagcgca gaacgataaa atggcacaat
     1021 ccaacaacaa ttttacattt tcgcgaccgc tttggctgct ttcaggtccg tttcaatgat
     1081 atactgccag tcgttaattc aaaaatagtt gataattaca acaatctatt gaattgaaac
     1141 gctttccttc gtaattcgca actggaacac gcacgctatg agtaaaccca ttgtgatgga
     1201 acgcggtgtt aaataccgcg atgccgataa gatggccctt atcccggtta aaaacgtggc
     1261 aacagagcgc gaagccctgc tgcgcaagcc ggaatggatg aaaatcaagc ttccagcgga
     1321 ctctacacgt atccagggca tcaaagccgc aatgcgcaaa aatggcctgc attctgtctg
     1381 cgaggaagcc tcctgcccta acctggcgga atgcttcaac cacggcacag caacgtttat
     1441 gatcctcggc gctatttgta cccgccgttg tccgttctgt gatgttgccc acggtcgccc
     1501 ggtagctcct gatgccaatg aaccagtgaa actggcgcag accattgccg atatggcgct
     1561 gcgttatgtg gttatcacct ccgttgaccg tgatgacctg cgcgatggcg gtgcccagca
     1621 ctttgcggat tgcattactg ccattcggga aaaaagcccg caaatcaaaa ttgaaactct
     1681 ggtgccggat ttccgtggtc gtatggatcg tgctctggat attctgactg caacgccacc
     1741 agatgtgttc aaccataacc tggaaaacgt accgcgtatt taccgtcagg tacggcctgg
     1801 tgcagattac aactggtcgc tgaagctgct ggaacgcttt aaagaagcgc atccggaaat
     1861 cccgaccaag tctggtctga tggtgggact gggtgaaacc aatgaagaaa ttattgaggt
     1921 aatgcgcgac ctgcgccgtc atggtgtgac gatgttaacg ctggggcaat atttgcagcc
     1981 aagccgccat cacctgccgg ttcaacgtta cgttagcccg gatgagttcg acgaaatgaa
     2041 agccgaagcg ctggcgatgg gctttaccca tgctgcatgc ggtccgtttg tccgctcttc
     2101 ttaccacgcc gatttgcagg cgaaagggat ggaagttaag taatcctgta ataattactt
     2161 acatctgtct gataaaaaac ccgctttttg
//
LOCUS       DAZRUF010000366         2174 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZRUF010000366 DAZRUF010000000
VERSION     DAZRUF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.369"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            174..2159
                     /gene="yjcS"
                     /locus_tag="ACHXOL_004184"
     CDS             174..2159
                     /gene="yjcS"
                     /locus_tag="ACHXOL_004184"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR6810993.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          582 a          572 c          527 g          493 t
ORIGIN      
        1 aataaattcg cgtaattttc cctcttcagc acaaatgcct aataaaatgg atcgttcagc
       61 tcacacttcc acttattaaa gtcgcaaatt cccaattatg ataaccccag ctttaaatga
      121 cgtattttgc atggattaca tatttacgac ataaatgata aatggagatt tacatgaata
      181 actctcggtt attccgtttg agcagaattg ttattgcgtt aactgccgcc agcggcatga
      241 tggtaaatac cgctaacgcg aaagaggaag cgaaagccgc cactcaatat acccaacagg
      301 ttaatcagaa ttacgccaaa tcattaccgt ttagcgatcg tcaggatttt gacgacgctc
      361 agcgtggatt tatcgctccg ctgctggatg aaggtattct gcgcgatgct aatggcaaac
      421 catattatcg cggggaagat tataaatttg atatcaatgc gcccgcgccc gaaaccgtta
      481 accctagcct gtggcgtcag tcgcagttaa acggtatttc cggcctgttt aaagtcaccg
      541 acagaatgta ccaggttcgc ggtcaggata tctccaacat cacctttatc gaaggtgata
      601 ccgggattat tgtcatcgac ccgctggtga cgccaccgag cgcaaaagcc gcccttgacc
      661 tttacttcca aaatcgcccg caaaaaccga ttgttgcggt tatttatacc cacagccata
      721 ccgaccacta tggcggcgtg aaaggcatta tctctgaagc tgatgtgaaa tccggtaaag
      781 tgcaggttat cgcccctgct ggctttatgg acgaagccat cagcgaaaac gtactggcgg
      841 gtaatattat gagccgccgt gcactttact cctacggcct gctgctggcg cataaccctc
      901 agggtaacat cggcaatggt cttggcgtaa cgctggcatc gggctacccg agcatcatcg
      961 caccgaacaa aaccatcacc aaaaccggtg agaagatgat tatcgacggc ctggagtttg
     1021 acttcctgat gaccccaggt agcgaagcac cagccgaaat gcacttctat attccggccc
     1081 tgaaagcgct gtgtaccgcc gagaacgcca cgcataccct gcacaacttc tacactctgc
     1141 gcggcgcgaa aacccgcgac accagcaagt ggaccgagta tctgaacgaa acgctggata
     1201 tgtggggtaa cgacgcggaa gtcctgttta tgccgcacac ctggccggtc tggggcaata
     1261 agcatatcaa tgattatatt ggtaaatatc gcgatactat caagtacatt cacgaccaga
     1321 ccctgcacct ggcgaaccag ggctacacca tgaatgaaat cggcgacatg attaaactgc
     1381 cgcctgcact tgccaataac tgggccagcc gtggctatta cggttctgtc agccacaacg
     1441 cccgcgcggt gtataacttc tatcttggct attacgacgg taacccggct aacctgcatc
     1501 cgtatggtca ggtggagatg ggtaaacgtt acgtgcaggc gctgggcggt tctgcccgtg
     1561 tcatcaacct ggcgcaagaa gcgaacaagc aaggtgatta ccgctggtcg gcagaactgc
     1621 tgaaacaggt gattgccgcc aacccgggtg accaggtcgc gaagaatctg caagcgaata
     1681 actttgaaca gctgggctat caggccgagt ccgccacctg gcgcggtttc tacctgaccg
     1741 gcgcgaaaga gctgcgcgaa ggggtgcata agttcagcca cggcaccatc ggttccccgg
     1801 acaccattcg cgggatgtcg gtcgaaatgc tgttcgactt tatgtccgtt cgcctcgata
     1861 gcgcgaaagc cgcgggtaaa aatatcagcc tgaacttcaa tatgagcaat ggcgataacc
     1921 tcaacctgac gctgaacgat agcgtgctta actaccgtaa aacactgcaa tcccaagctg
     1981 acgcctcttt ctacatcagc cgtgaagatc tgcacgccgt gctgaccggg caagccaaaa
     2041 tggcggatct ggtaaaagcg aagaaagcca aaattattgg caatggcgcg aaactggaag
     2101 aaattatcgc ctgtctggat aatttcgatt tgtgggtgaa tatcgtaacc ccaaattaat
     2161 gtcactcgct tttg
//
LOCUS       DAZRUF010000367         2173 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZRUF010000367 DAZRUF010000000
VERSION     DAZRUF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.370"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(78..197)
                     /locus_tag="ACHXOL_004185"
                     /pseudo
     CDS             complement(78..197)
                     /locus_tag="ACHXOL_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
     gene            complement(302..1804)
                     /gene="dtpA"
                     /locus_tag="ACHXOL_004186"
     CDS             complement(302..1804)
                     /gene="dtpA"
                     /locus_tag="ACHXOL_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR6810994.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT          610 a          531 c          524 g          508 t
ORIGIN      
        1 ccggaaacaa gtgagcgttt ccggattctt acacagccac tggatcggtc aactgatcct
       61 taactgatcg gcattacacc gttttcgaga cgttttttca ttaaatccac actgacgagg
      121 gtaaaatcct ttccgctctc acgcagggtg atatgggaag cgagagagca ggcacccggt
      181 ttgtagaaca atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt
      241 gctgaacaaa aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt ttccctgaat
      301 tttacgctac agcagctttc gccgctttgt ctgcagcgtc atcctgcgtc atacggtgca
      361 gtttaggcgc ggtcagcagc atcagtactg caataatggc agtagcgaca ccaatctgca
      421 agaatacgcg accataaact tccagtgaca tcagcgggtc ggtaacgtta tccggcacag
      481 ccatcatacc cgcaacataa ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc
      541 tacccataat gaagcccatc agacgctgcg gaacgagttg tgcaaccatt gccagaccca
      601 gaccagagat catcagttcc ccgatgctct gcaggccata gcttgcgacc agccagctta
      661 cagacacgat accagcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac
      721 cagaacacat caccatgccg attgcaaact tggttggcat cggcagggta tcgcccatct
      781 tgttatagat agcggccaga atcggactac cgatgatgat ccagaacggg ttcagtgcct
      841 gatactgctc aggttctacg gccagaccca gaatggagtg ctcaacgtta cgaatcgcaa
      901 agaagttcag tgacgttggc atctggctgt acagcacgaa gaagataatg gcttcgagca
      961 tcaggatgaa ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaggcttctt
     1021 taccgaagat aaccacgata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct
     1081 gattgtgcag cagccaggtg gcgatagcga tcagtgccac aacaccaata atggtcagca
     1141 gcaggttacg gtagttgata ggctcgaagt ctggttttga accgtactgt ttaacccagc
     1201 gttggcagaa ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg
     1261 caacactcca gccgtatttc gcggccagcc acggcgtagc aatcatagag aagaaagagc
     1321 cgatgttgac ggacatgtag tacatggtga atgcaccgtc cagacgcggg tcgtttttct
     1381 catagcatgt agaaagcaga gaagatgggt tagctttaaa caggccgtta ccgaccgcaa
     1441 tagccgccat acccatataa acgataccgg cgtcgtgacc agaccaggca accagagcat
     1501 aaccaatcgc cagcacaata gcgccgagca taattacgcg tttagtaccc agtaccttgt
     1561 cacctaacca gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa
     1621 gggtgattga atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc
     1681 cttgtaggcc gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg
     1741 ccttcggttg tttgaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg
     1801 acacttttac ctcttttttt acatcccata ttaacggggg ggctttctcg cgttatgtct
     1861 aaaaaacata cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct
     1921 tgcactttca gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca
     1981 accattccgg gaaatgttat ccagcgttaa atttttcaaa aaacgtaatg gcagattttt
     2041 tcactgcact gagagggggg gtgatggctt aacggcgata tgttctgcta tttggcgttt
     2101 ttactagtga gatagtccag tttctgaaaa atagccagtg taatgttttg taggtcaata
     2161 tttgttgcgt gtg
//
LOCUS       DAZRUF010000368         2164 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZRUF010000368 DAZRUF010000000
VERSION     DAZRUF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.371"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            105..1622
                     /gene="lysS"
                     /locus_tag="ACHXOL_004187"
     CDS             105..1622
                     /gene="lysS"
                     /locus_tag="ACHXOL_004187"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR6810995.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(1741..1914)
                     /gene="ghoT"
                     /locus_tag="ACHXOL_004188"
     CDS             complement(1741..1914)
                     /gene="ghoT"
                     /locus_tag="ACHXOL_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR6810996.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(1942..>2164)
                     /gene="ghoS"
                     /locus_tag="ACHXOL_004189"
     CDS             complement(1942..>2164)
                     /gene="ghoS"
                     /locus_tag="ACHXOL_004189"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR6810997.1"
                     /translation="RLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDL
                     IRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
BASE COUNT          615 a          465 c          499 g          585 t
ORIGIN      
        1 tcgataagat aaactgagtt attaatagtc gaggcagata atacagtgta ccgatctgtc
       61 tctttatcta cgctaaattg aaagctggat ttagaggaac caaaatgtct gaacaagaaa
      121 cacggggagc caatgaggct attgatttta acgatgaact gagaaatcgc cgcgaaaaac
      181 tggcggcact acgtcagcaa ggtgtggcgt ttcccaatga ttttcgccgc gaccatacct
      241 ctgaccagtt gcacgaagag tttgatgcga agaataacca ggaactggaa tccttaaaca
      301 ttgaagtctc ggttgctggc cgaatgatga cccgtcgtat catggggaaa gcctcctttg
      361 taacgttgca ggatgtcggt ggccgtattc aactgtacgt tgcaagagat agcctgccag
      421 aaggtgttta taacgatcag tttaaaaaat gggatctggg tgacattatc ggtgcccgcg
      481 gtacgctgtt taagacgcaa acgggtgagc tttccattca ctgtactgag ttgcgcctgc
      541 tgactaaagc actacgtcct ttaccagata aattccatgg tctgcaggat caggaagtcc
      601 gttatcgtca acgttatctg gacctcatcg ctaacgataa atcccgtcaa acgtttgttg
      661 tccgttcaaa aattctggcc gctatccgtc agttcatggt cgcgcgcggc tttatggaag
      721 tagaaacccc gatgatgcag gtaattccag gtggggcatc tgctcgcccg tttattaccc
      781 atcataatgc tctggattta gatatgtatc tgcgtatcgc gccggagctg tatctgaaac
      841 gtctggttgt aggcggtttt gaacgggtat tcgaaatcaa ccgtaacttc cgtaatgaag
      901 gtatttctgt tcgccataat cctgagttca caatgatgga actctacatg gcgtatgcgg
      961 attaccacga tttgattgaa ctgacagagt cactgttccg caccctggca caagaggttc
     1021 tgggtaccac taaagtcact tatggcgagc atgtgtttga tttcggcaaa ccgtttgaaa
     1081 aactcaccat gcgcgaagca atcaaaaaat atcgtccaga aaccgatatg gccgacctgg
     1141 ataattttga tgctgctaaa gcattagctg aatctatcgg aattacggta gagaaaagct
     1201 gggggctggg acgtattatc acggagatct ttgatgaagt ggcagaagca catctgattc
     1261 agccaacctt tattacggaa tatccggcag aagtgtcacc gctggcgcgc cgtaatgatg
     1321 ttaacccgga aatcaccgac cgttttgaat tcttcatcgg tggtcgtgaa atcggtaatg
     1381 gttttagcga attaaacgac gcagaagatc aggctgaacg tttccaggaa caggttaacg
     1441 ctaaagctgc aggtgacgac gaagccatgt tctatgacga agattacgta actgcgctgg
     1501 aatatggtct gccgccaacc gctggtctgg gtattggtat cgaccgaatg attatgctgt
     1561 ttacaaacag tcatactatt cgcgacgtta ttctcttccc ggcgatgcgc ccacagaaat
     1621 aaatttcact ttaatgaacg aagcagtcag gcgactgctt cgttcgtcgt tccgcaaatc
     1681 cagttttgag gagagacgct tcgtttcacg ataaccagcg ggtccactca actacagctc
     1741 ctaaaagaga gaaaaaagta atgccacagg cagactcatt ggccaggtta ttccgaccag
     1801 gaatgcacta agtaaacgaa tatgtgtttt ctcatgtgag ataaaccaga taatgacaaa
     1861 ggatatgttc acaccaatca cataaaaaat taatatttta gagaatagtg ccatttttta
     1921 tgttcttctt atccttcctg gctacttgta aaactgacgg aaatagtcat caactgtcat
     1981 aatgttgagg tcaaaatcaa tatccggtaa gtctaagcat ttaagcctga ttaaatcttc
     2041 gatttcactt gcggctaacg atgtcgttat tgcgaaggaa ttttcattca attgtcgtgg
     2101 tatcccatat tcatcagcca cgatagaaga gaaattcata tcatacatca atgatcttaa
     2161 cctt
//
LOCUS       DAZRUF010000369         2113 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZRUF010000369 DAZRUF010000000
VERSION     DAZRUF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.372"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            82..591
                     /locus_tag="ACHXOL_004190"
     CDS             82..591
                     /locus_tag="ACHXOL_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR6810998.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            588..2006
                     /gene="phrB"
                     /locus_tag="ACHXOL_004191"
     CDS             588..2006
                     /gene="phrB"
                     /locus_tag="ACHXOL_004191"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR6810999.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
BASE COUNT          504 a          527 c          570 g          512 t
ORIGIN      
        1 tacttccccg caaaaactct gccccataaa attcatttac catcgtctac acttactcct
       61 ttataccgtt caggagcagt aatgaatcta caacgatttg atgacagcac cctaatccgt
      121 atctttgccc ttcatgagtt acatcgactg aaagaacatg gcttaacgcg cggggcgctt
      181 ctcgattatc acagccgcta taaactcgtc tttctggcgc attctcagcc ggagtaccgc
      241 aaacttggcc cgttcgtggc tgatattcac cagtggcaaa atctggatga ctattacaac
      301 cagtaccgcc aacgcgtagt tgttttgctt tctcaccccg ccaacccgcg cgatcacacc
      361 aatgttttga tgcacgttca gggttatttt cgcccgcata ttgattccac agaacgccag
      421 cagctggctg cgcttatcga cagttatcgc cgtggcgagc aaccacttct tgcgccgctg
      481 atgcgtatca aacactatat ggcgctttat catgacgcct ggctttcagg gcagcgttat
      541 ttcgaacttt ggccgcgtgt gattaacttg cgccattcag gagttttatg actacccatc
      601 tggtctggtt tcgccaagat ttacgtctgc acgataatct cgcactggct gccgcctgcc
      661 gcaattcgtc tgcacgcgtg ctggcgttgt atatcgctac accacgccag tgggcgacgc
      721 ataacatgtc gccgcgtcag gctgaactta tcaatgctca actgaatggg ctacaaatag
      781 cgcttgcgga aaaaggtatt cctttattgt tccgtgaagt ggatgacttt gtcgccagtg
      841 tcgaaatagt taaacaggtg tgcgcggaaa acagcgttac ccacctgttt tataactatc
      901 agtatgaagt gaatgagcgg gcgcgggatg tggaagttga aagagcgctg cgtaacgtgg
      961 tgtgtgaagg atttgatgac agcgtgatcc tgccgcctgg cgcggtgatg accggtaatc
     1021 acgagatgta caaagtcttt acgcctttta agaatgcctg gctgaaacgg ctgcgggaag
     1081 ggatgccgga gtgcgtcgct gcgccaaaag ttcgtagtag cggatcgata gagccctcgc
     1141 catccattac gctgaattat cctcgtcagt ctttcgatac tgcgcatttt ccggtggaag
     1201 aaaaagcggc gattgcgcaa ttacgccagt tttgccagaa cggtgccgga gaatatgagc
     1261 aacaacgaga ttttccggca gtggaaggca ccagccgttt gtcggccagc ctggcaacgg
     1321 gcgggttatc gcctcgccag tgcttgcatc gcttgttggc tgaacagccg caggcgctgg
     1381 acggtggggt cggtagtgtc tggcttaatg agctgatctg gcgcgagttt taccgtcacc
     1441 tgataacgta tcacccctcg ttgtgtaaac atcgtccaat tattgcctgg acggatcgtg
     1501 tacagtggca gagcaatccc gcacatttac aggcctggca ggaaggcaaa acgggatacc
     1561 cgattgttga tgccgctatg cgtcagctta acagcactgg ctggatgcat aacaggctac
     1621 ggatgattac agccagtttt ctggtgaaag atttattgat cgactggcgc gaaggcgagc
     1681 gatatttcat gtcgcagctg attgatggtg atttggcagc caataacggt ggctggcagt
     1741 gggccgcttc aaccggaacc gatgcagcgc cgtattttcg tattttcaac ccgacaaccc
     1801 agggcgagaa atttgatctc gaaggtgagt ttatccgcca gtggctaccg gaactacgca
     1861 atgtaccggg gaaatcggtg catgagccgt ggaagtgggc gcagaaagca ggtgtgaagc
     1921 tggattatcc gcaaccgata gtcgagcaca aagaggcgag agtacaaacg ttggcagcgt
     1981 atgaggcggc gcggaagggg aaataacaga cgcgtcaggc aatcgagccc agatgccgga
     2041 tgcggcgtga acgccttatc cagcctacaa atttgtgcaa attcaataaa ttgcaggaaa
     2101 tacgtaggcc tga
//
LOCUS       DAZRUF010000370         2103 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZRUF010000370 DAZRUF010000000
VERSION     DAZRUF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.373"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..547
                     /locus_tag="ACHXOL_004192"
     CDS             <1..547
                     /locus_tag="ACHXOL_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811000.1"
                     /translation="QIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQVEKVGIIRALT
                     VGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTG
                     QTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQ
                     PVPDAPVPKDLPPGMPDMRQF"
     gene            623..>2103
                     /locus_tag="ACHXOL_004193"
     CDS             623..>2103
                     /locus_tag="ACHXOL_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR6811001.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKA
                     PFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLS
                     ALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLTVVVDGFGRSLTFHRAA
                     EGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASRKQRASSLSSPASPRSVSSSQVFPD
                     TLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVYDRSGTQV
                     RGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITD
                     SLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMA
                     SGKLTSVVLPDGRTVRYGYNNQLQLTSVTYPDGLRSSRKYDRQG"
BASE COUNT          443 a          536 c          733 g          391 t
ORIGIN      
        1 ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc aggtggaaac
       61 ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg tggggatcat
      121 tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta tgaatacctc
      181 ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg tgggaatggg
      241 ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga tgaaggaaaa
      301 caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct gtggtaaggc
      361 aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca ttcatctgga
      421 aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg gtgccacaca
      481 acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg atatgcggca
      541 attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga tgaaccatat
      601 taaaagcaga aggaattgat tcatgggcgg cggaaaaccg gcggcgcggc agggcgacat
      661 gacccgcaag ggactggata ttgtgcaggg ttcagcgggt gtgctgatag gtgcgccgac
      721 gggagtggcc tgttcggtgt gtccgggagg gattacctat gctaacccgg tgaacccggt
      781 gaacccggtg ctgggtgcga aggtgcttcc gggcgagacg gaccttgcgc tgccctgccc
      841 gctgccgttt attcttttcc gtgcctacag cagctaccgg accagaacac ccgcgccggt
      901 gggggtgttt ggtcccggct ggaaagcgcc cttcgatatc cgcttacaga tacgcgatga
      961 aggcctgata ctcaacgaca gcggcggcag gagcattcac tttgagccgc tgtttcccgg
     1021 cgagataagc tacagccgca gcgagtcact ctggctggcg cggggcggag tggcggcgca
     1081 gcacagttcg cagccgctaa gcgcgctctg gcaggtgctg ccggaagatg ttcgcctgag
     1141 tccgcatgtg tacctggcga caaacagcct tcaggggccg tggtggatac tcagctggcc
     1201 ggagccgccc gcataccggg tactgacggt cgtggtggat ggcttcgggc gctcactgac
     1261 tttccaccgg gcggcggagg gtgacgtggc gggcgcggtg acgggggtga cggacggcgc
     1321 gggccgccgt tttcacatgg cgctgagcac gcaggcgcag cgggcggaag cgtcccgtaa
     1381 acagcgcgcc tcttccttat cttccccggc cagtccccgt tctgtttcct cttctcaggt
     1441 tttccccgac acgctgcccg ccggtacaga gtacggtgcc gataacggta tccggctgga
     1501 ggcggtatgg ctgacacacg acccggcata cccggatgaa cagccgactg cgccgctggc
     1561 gcgctacacg tacacggccg gtggtgaact gcgggcggtg tatgaccgca gcgggacgca
     1621 ggttcgcggg tttacttatg atgcggagca cgccgggcgg atggtggcgc accattatgc
     1681 ggggaggccg gagagccgct accggtatga tgataccggt cgggtgacgg agcaggtcaa
     1741 cccggagggg ctggactacc gctttgagta cggagagagc cgggtaatca tcacggacag
     1801 cctgaaccgg cgggaggtgc tgtacacgga aggcgagggt ggcctgaagc gggtggtgaa
     1861 gaaggagcat gccgacggga gcatcacccg cagcgagtat gatgaggcgg ggaggctgaa
     1921 ggcgcagacg gatgcggcgg ggcggcggac ggagtaccgg ctgcatatgg cgtctggaaa
     1981 gctgacttca gtggtactgc cggacggcag gacggtacgg tatggttaca acaaccagtt
     2041 gcaactgacg tcagtgacat atccggatgg tttacgcagc agccggaaat atgaccgtca
     2101 ggg
//
LOCUS       DAZRUF010000371         2089 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZRUF010000371 DAZRUF010000000
VERSION     DAZRUF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.374"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHXOL_004194"
     CDS             complement(60..1025)
                     /locus_tag="ACHXOL_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR6811002.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHXOL_004195"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHXOL_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811003.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          550 c          436 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZRUF010000372         2088 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZRUF010000372 DAZRUF010000000
VERSION     DAZRUF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.375"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="ACHXOL_004196"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="ACHXOL_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR6811004.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="ACHXOL_004197"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="ACHXOL_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR6811005.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          552 a          505 c          590 g          441 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAZRUF010000373         2063 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZRUF010000373 DAZRUF010000000
VERSION     DAZRUF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.376"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            240..695
                     /locus_tag="ACHXOL_004198"
     CDS             240..695
                     /locus_tag="ACHXOL_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR6811006.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            695..865
                     /locus_tag="ACHXOL_004199"
     CDS             695..865
                     /locus_tag="ACHXOL_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR6811007.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            858..1148
                     /locus_tag="ACHXOL_004200"
     CDS             858..1148
                     /locus_tag="ACHXOL_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR6811008.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1145..1507
                     /gene="rusA"
                     /locus_tag="ACHXOL_004201"
     CDS             1145..1507
                     /gene="rusA"
                     /locus_tag="ACHXOL_004201"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR6811009.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1504..1644
                     /locus_tag="ACHXOL_004202"
     CDS             1504..1644
                     /locus_tag="ACHXOL_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR6811010.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1730..>2063
                     /gene="quuD"
                     /locus_tag="ACHXOL_004203"
     CDS             1730..>2063
                     /gene="quuD"
                     /locus_tag="ACHXOL_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR6811011.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLM"
BASE COUNT          536 a          481 c          548 g          498 t
ORIGIN      
        1 gattaggtga aagtatacaa ggtttttaaa gcgaagatca ctgttttggc ttcaagatag
       61 ttttgattta tcgtaatcaa tccgccataa tcctctcatc ggagcctgaa caactccggt
      121 gacttctgcg catttaaggg gacttaaatg cgaccacaat ctgaactcat caccttgtca
      181 cagatgcaga aatgcacctg cgattttctg cattctgcgg tttccgttag ggaggccgta
      241 tgactctgcc agtagacggc atcaaactcc atcgcggtaa cttcgcggcc atcggccagc
      301 agattcagcc attgctggat gccgggcaat gcttacgcct gcaggttaag ccgtggcgcg
      361 agaagcgcag cctgtcgcag aacgcgctca gccacatgtg gtacacggag atcagcgaat
      421 atctcatcgc ccgcggcaag accttcgcta cgcctgagtg ggtcaaagac gcgatgaagc
      481 acacctatct cggctacgaa agcaaagacc gtgtagatgt cgtgtccggc gaggtcacca
      541 ccgtccaatc cctccgccat acgtcagagc tggaaaccgg cgagatgtac atcttcctgt
      601 gcaaagtcga agcctgggcg atgaatatcg gctgccacct gacaattccg cagagctgtg
      661 aataccagca gctgcgcgat aagcaggagg cgtaatggct acaccgctta ttcgtgtcat
      721 gaacgggcac atctacagag tatcaaatcg tcgtaagcgt aagcctgagc tgaagccatc
      781 cgaaatacca acactgctcg gatataccgc tagcctggtt gataaaaaat ggttgcgact
      841 ggcagcaagg aggaatcatg gctgatttga gaaaagcagc gcgtggtcgg gaatgccagg
      901 taagaatccc tggcgtatgt aatggcaatc ctgaaacgtc agtactggca catatccggc
      961 tggctggatt gtgcggtacc ggtatcaaac cgccagacct gattgccacc attgcatgtt
     1021 ctgcctgtca cgacgaaatc gaccgccgca catattttgt cgatgctgag tatgcaaaag
     1081 aatgcgcgct ggaaggtatg gcgagaacgc aggttatctg gctgaaagag ggggttatta
     1141 aggcgtgaat acctacagca tcacattacc ctggcctccg agcaataacc gctattaccg
     1201 ccataatcgc gggcgcacac acatcagcgc agaagggcag gcataccgcg ataacgtcac
     1261 ccgaatcatt aaaaacgcaa tgctggatat cggcctggct atgccagtga aaatccgtat
     1321 tgagtgccac atgccggatc gccgtcgccg tgacctggat aatctgcaaa aagccgcttt
     1381 tgacgcactc accaaagcag gtttctggct ggatgatgct caggtcgttg attaccgcgt
     1441 tgtgaagatg cctgttacca aaggtgggag gctggaactg accatcaccg aaatggggaa
     1501 tgaatgatgt ttgagtttta tatggcagaa cttcttcgcc accgctgggg gcatctgcgc
     1561 ttatatcgtt tccccggttc tgttttgacc gattaccgaa tactgaagaa ttacgccaaa
     1621 accctgacag gagcaggagt atgaagtcag agataacaat caactaatac tgttttgttg
     1681 atttttgctt gtaattggcg ttctggtctg atttttgtgg agtaagttga tgcgtgatat
     1741 tcagatggtt cttgagcgtt ggggagcgtg ggcggctaat aatcatgaag atgtgacctg
     1801 gtcgtccatt gccgccggtt ttaagggatt aattacttca aaagtaaaat ctcgcccgca
     1861 atgttgtgac gatgacgcga tgattatttg cgggtgcatg gcccgtctga aaaagaacaa
     1921 cagcgatttg cacgatttat tagtagatta ttatgtagtc ggtatgacat tcatgtcact
     1981 ggcaggtaag cattgctgct ctgatggtta tatcgggaaa aggttacaga aggctgaggg
     2041 cataattgaa gggatgttaa tgg
//
LOCUS       DAZRUF010000374         2020 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZRUF010000374 DAZRUF010000000
VERSION     DAZRUF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.377"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            211..1746
                     /gene="istA"
                     /locus_tag="ACHXOL_004204"
     CDS             211..1746
                     /gene="istA"
                     /locus_tag="ACHXOL_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR6811012.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYG
                     PERLEKACYVALHYNAPDRRFIDNLLRHHRDNVELPLSRQGEQHPAYASEHENLRGPG
                     YYH"
     gene            1763..>2020
                     /gene="istB"
                     /locus_tag="ACHXOL_004205"
     CDS             1763..>2020
                     /gene="istB"
                     /locus_tag="ACHXOL_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR6811013.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQ"
BASE COUNT          521 a          555 c          515 g          429 t
ORIGIN      
        1 atcgcagttt ggcattcgaa tcgatcatga tttggcatcc atctccgatc acagtttggc
       61 atcacaatcg atcacgattt ggcatacttc cgatcattga ttggaattcg ccagtcactc
      121 cgggaattaa ctcttttcac cacagtctcc attgccgtgt ttaaaccagt ggagacgtca
      181 atgtccaaaa agagaaaatc caggagcact atggaaactt gttttaagat ccttcagctc
      241 aagttcgata agaagctgac taaccgttgt atcggactga cgttacacat cagcgcctca
      301 accgtttttg aggttcttgc ccgcttcaaa gcctcttctc tgtcatggcc attacctgcg
      361 gatatatccc atgacacgct ggagaaactc attttcccgc ccaaagacac ctctgcgtca
      421 gagcttgtta tgcctgacat gctgtacttc gatacggaaa tgagaaaacc tggcgtgacg
      481 cgccaactgt tgtggatgga atacaaggca caggcgggtg acaaagcgat gggatactca
      541 cacttctgcc gttgctaccg gaagtggaaa aaaacgcgac gcctctccat gcgacaggag
      601 catcgtgctg gcgaaaagct ctttatcgac ttctgtggtc ctacggtccc tgttattaat
      661 ccggacaccg gagagatacg ccgcgtggca atcttcgtgg ctgtaatggg ggcgtccaac
      721 tatacctatg ttgaagcgtg cgaaggtcag gacatgatgt catggttaaa tgcccacagc
      781 cgttgcctga ccttcctggg cggcgtaccg aagctgctca tccccgataa cctcagaagc
      841 gcagtgaaaa aagccgatcg ctatgagcct gtcatcaacg acagttatca ggcgctggct
      901 gagcactatg gcacggttat tatcccggcc cgcccgcgca aacctaaaga taaaccaaaa
      961 gcagaaaacg gcgtcctcat cgtcgagcga tggcttcttg cccgcatccg taatgaaacc
     1021 ttccacacgc tcagggcgct gaatgcacgc cttcgggagt tgctgacgga catgaataac
     1081 cgaccaatga agggatacgg taatcaaacc cgcgcagaac gcttccgcat gctggatgca
     1141 ccagcactct ctccgctgcc tctggagcct tatgaataca cggaatacaa agccgtgaag
     1201 gttggccctg actaccacgt tgaatatgcc cgtcactggt actccgttcc acacgaactg
     1261 gtcggtcagc gtctgtcact gaaggtgggg cagtcggtgg tacagctctg gcacaagggg
     1321 cagtgtgtgg cgcaacatcc gcgcagcaca catgaatata aacacacaac caatccgctt
     1381 cacatgccag aacgccaccg gcggcatggg acatggacac cggaacgcct tatcgagcag
     1441 ggaaaccgta caggtccgtc cacaggccgg gtggttgaat ccatgctgaa agccaaaccc
     1501 catccggagc tggcttaccg tgcagtcctg ggtctgctgg cacttcagaa aaaatacggc
     1561 ccggaacggc tggaaaaggc ctgttacgtt gccctgcact ataacgcccc ggacagacgc
     1621 tttatcgaca atctgctgcg tcatcatcgg gataacgttg agcttccgtt atcccgccag
     1681 ggcgaacaac atccggcata tgcttcagag catgaaaacc tgcgtggtcc tggctactac
     1741 cactgacaga aggaacaacc gtatgagtga taatctgtta aacaaactga cccaactgaa
     1801 actcccggcg atggccggga gcctgatacg gcaacgggaa acgcctcaga cttacgacga
     1861 actgtcgttc gaggaaaggc tgacactgct ggttgatgac gagcttctga gcagagaaaa
     1921 cagtcgtgtg gcacggttga gaaaaaatgc ctgcctgaaa tatcaggcaa caccggaagg
     1981 gctgcgttat ccggcctcaa gaggtctgcg ggctgaacag
//
LOCUS       DAZRUF010000375         2004 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZRUF010000375 DAZRUF010000000
VERSION     DAZRUF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.378"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(126..596)
                     /gene="argR"
                     /locus_tag="ACHXOL_004206"
     CDS             complement(126..596)
                     /gene="argR"
                     /locus_tag="ACHXOL_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR6811014.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            1031..1969
                     /gene="mdh"
                     /locus_tag="ACHXOL_004207"
     CDS             1031..1969
                     /gene="mdh"
                     /locus_tag="ACHXOL_004207"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR6811015.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT          520 a          436 c          517 g          531 t
ORIGIN      
        1 tattcattgt gtgaatgata tgtcgcggta aaacgcacta ttcgttaaat taatcgcagg
       61 ggaaagcagg gggttgagag gaataagcaa cattttcccc gccgccagaa gcgacggggc
      121 agagattaaa gctcctggtc gaacagctct aaaatcgctt cgtacaggtc tttgacggtg
      181 aaaccgttag caggggtggt aaagatggtg tcatcgccag cgatggtgcc cagaatacct
      241 tctgctttgc ccagtgagtc cagcaggcga gcaattaact gcgccgcgcc agggctggta
      301 tgaatcacga caactgcatc gttgtagtcg atatccagca ccagattctt caatggactg
      361 gaggtggttg gtacacccag ttcagctggc aggcagtaaa ccatttccat tttggcattg
      421 cgtgtacgta cagcaccaaa cttggtcaac atccgcgaga ctttagactg attaatattg
      481 tcaaagcctt gctcctgcaa cgcggcgacg atttcgccct gggagctaaa tttctcttct
      541 ttaagtaatg ctttaaatgc tttaactagt tcttcttgct tagccgagct tcgcataagt
      601 cacccgatat ggtggttgat acaacattat tgtgcataca gatgaatttt tatgcaaaca
      661 gtcagccctg aagaaggctg aaataatgtt atgaaagagc gggattttat caaatttcgt
      721 tattgagaaa catgcctgcg tcacggcatg caaattctgt ttaaaagtaa attaattgtt
      781 atcaaattga tgttgttttg gctgaacggt agggtatatt gtcaccacct gttggaatgt
      841 tgcgctaatg cataagcgac tgttaattac gtaagttagg ttcctgatta cggcaattaa
      901 atgcataaac gctaaacttg cgtgactaca cattcttgag atgtggtcat tgtaaacggc
      961 aattttgtgg attaaggtcg cggcagcgga gcaacatatc ttagtttatc aatataataa
     1021 ggagtttagg atgaaagtcg cagtcctcgg cgctgctggc ggtattggcc aggcgcttgc
     1081 actactgtta aaaacccaac tgccttcagg ttcagaactc tctctgtatg atatcgctcc
     1141 agtgactccc ggtgtggctg tcgatctgag ccatatccct actgctgtga aaatcaaagg
     1201 tttttctggt gaagatgcga ctccggcgct ggaaggcgca gatgtcgttc ttatctctgc
     1261 aggtgtagcg cgtaaaccgg gtatggatcg ttccgacctg tttaacgtta acgccggcat
     1321 cgtgaaaaac ctggtacagc aagttgcgaa aacctgcccg aaagcgtgca ttggtattat
     1381 cactaacccg gttaacacca cagttgcgat tgctgctgaa gtgctgaaaa aagccggtgt
     1441 ttatgacaaa aacaaactgt tcggcgttac cacgctggac atcattcgtt ccaacacttt
     1501 tgttgcggaa ctgaaaggca aacagccagg cgaagttgaa gtgccggtta ttggcggtca
     1561 ctctggtgtt accattctgc cgctgctgtc acaggttcct ggcgttagtt ttaccgagca
     1621 ggaagtggct gatctgacca aacgcatcca gaacgcgggt actgaagtgg ttgaagcgaa
     1681 agccggtggc gggtctgcaa ccctgtctat gggccaggca gctgcacgtt ttggtctgtc
     1741 tctggttcgt gcactgcagg gcgaacaagg cgttgtcgaa tgtgcctacg ttgaaggcga
     1801 tggtcagtac gcccgtttct tctctcaacc gctgctgctg ggtaaaaacg gcgtggaaga
     1861 gcgtaaatct atcggtaccc tgagcgcatt tgaacagaac gcgctggaag gtatgctgga
     1921 tacactcaag aaagatatcg ccttgggcga agagttcgtt aataagtaat tgattagcga
     1981 ataataaaaa accggagcac agac
//
LOCUS       DAZRUF010000376         2003 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZRUF010000376 DAZRUF010000000
VERSION     DAZRUF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.379"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..387
                     /locus_tag="ACHXOL_004208"
     CDS             <1..387
                     /locus_tag="ACHXOL_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811016.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(412..750)
                     /gene="maoP"
                     /locus_tag="ACHXOL_004209"
     CDS             complement(412..750)
                     /gene="maoP"
                     /locus_tag="ACHXOL_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR6811017.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            869..1708
                     /gene="hdfR"
                     /locus_tag="ACHXOL_004210"
     CDS             869..1708
                     /gene="hdfR"
                     /locus_tag="ACHXOL_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR6811018.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1804..1879)
                     /locus_tag="ACHXOL_004211"
     tRNA            complement(1804..1879)
                     /locus_tag="ACHXOL_004211"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1844..1846),aa:Trp,seq:cca)
     gene            complement(1888..1964)
                     /locus_tag="ACHXOL_004212"
     tRNA            complement(1888..1964)
                     /locus_tag="ACHXOL_004212"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1928..1930),aa:Asp,seq:gtc)
BASE COUNT          529 a          483 c          486 g          505 t
ORIGIN      
        1 ttcgatctct cactggagat cccattacca ccccccggca ttttgagtaa aacggtagtg
       61 ccgggagaaa gcagcgccac cgttaaacaa cgcgtaatgg ccgccagaga gcgccaattt
      121 aagcggcaga ataaactgaa tgcctggctg gatagtccgg aaatacgcca attctgcaaa
      181 cttgagagcg aagatgcgat gtggttggaa ggaacactga tccatctggg gttatcgatt
      241 cgtgcctggc agcggttatt gaaagttgca cgaaccattg ctgatattga tcagtctgac
      301 attatcacac gtcagcattt gcaggaggca gttagctatc gtgcgattga ccgtttgctc
      361 atccatctgc aaaaactact gacataaaaa aagggcattt cgcccttttt attaatcatc
      421 agaatcggtg tagtcttcag caccttcaac ctgcggttta ccgccggaaa gggtgtgaaa
      481 acgttttgga cgcttaatac gcgtcatata cttggaccac acgcgttctg cttctgtcac
      541 tggctcacgt tcgccacggc atactgctac gaagagtttc tcttcctcgg taaccggctc
      601 gcgtttgcca agatccaact cattgaaggc ataaccatga cgctcaagca gttgtgcctc
      661 tttgatggtg aaatcaccat gacgagagaa tccacgtgga taatgtttat tgtcgaaata
      721 tcgattagtc gtcgtaaagc tttccgccat cctgcacgct cctaattctt tgaccgagct
      781 agttatggcg cggagtatta gttacgcttg acagagtgta aaacaaaaca tttaaatcat
      841 aacgacaaat aattttgcgg agagcactgt ggatacggaa ttgttaaaaa ctttcctgga
      901 agttagccga acgcgtcact ttggtcgagc ggctgaatcg ctctatctga cccagtcagc
      961 agtgagcttt cgaatcaggc aactggaaaa tcaactgggt gtgaaccttt tcacccgcca
     1021 cagaaacaat atccgtttaa ccgctgccgg tgaaaaacta ctgccttatg cagaaacgct
     1081 catgagcacc tggcaggccg cccgcaagga ggtggcgcat acctcacgac ataacgagtt
     1141 ttctatcggt gccagcgcct cgttgtggga atgtatgctt aatcagtggt tgggacgctt
     1201 gtatcaaaat caggatgccc atacaggctt acagttcgaa gcgcgaattg cccaacggca
     1261 gtctctggta aaacagctgc atgaacgcca gcttgatctt cttattacca ctgaagcgcc
     1321 caaaatggac gaatttagta gtcagttgct ggggtatttc actttagcgc tttataccag
     1381 tgccccttct aaactaaagg gagatcttaa ctatctgcga ctggagtggg ggccagattt
     1441 tcaacagcat gaggcaggtt tgatcggtgc tgacgaagtt cccattctga caaccagttc
     1501 tgctgaactg gcacagcaac agattgcgat gcttaatggt tgcacctggc tacccgttag
     1561 ctgggcgcgt aaaaaaggcg gcctgcatac cgttgtcgat agcacaacac tttcacggcc
     1621 gctttatgcc atatggctgc aaaatagcga taaaaatgcg ttgattcgcg atcttttgaa
     1681 aattaacgtg ctggatgaag tgtattaatc tgaatggctg gcaaggatgc cggtagaagg
     1741 atttacttcg gagaggatta tttcagataa aaaaaatcct tagctttcgc taaggatgat
     1801 ttctggcagg ggcggagaga ctcgaactcc caacacccgg ttttggagac cggtgctcta
     1861 ccaattgaac tacgccccta attagggtgg cggaacggac gggactcgaa cccgcgaccc
     1921 cctgcgtgac aggcaggtat tctaaccgac tgaactaccg ctccaccgaa ttcttttaca
     1981 accaccggtt ttatgaccgg ctt
//
LOCUS       DAZRUF010000377         1983 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZRUF010000377 DAZRUF010000000
VERSION     DAZRUF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.380"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHXOL_004213"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHXOL_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR6811019.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHXOL_004214"
     CDS             complement(390..1178)
                     /locus_tag="ACHXOL_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR6811020.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSWIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHXOL_004215"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHXOL_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR6811021.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHXOL_004216"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHXOL_004216"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR6811022.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT          502 a          524 c          457 g          500 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cagctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggt
//
LOCUS       DAZRUF010000378         1973 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZRUF010000378 DAZRUF010000000
VERSION     DAZRUF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.381"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..266)
                     /locus_tag="ACHXOL_004217"
     CDS             complement(<1..266)
                     /locus_tag="ACHXOL_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFR6811023.1"
                     /translation="MLKQRRNFCTGTERHANRFTTSASRSNIRYSLSDTHATPDGYPV
                     KQIGEHAWLIEKAGIVIHKCPRNPFTGNRIFALSCGDNQFGQDFT"
     gene            complement(281..559)
                     /locus_tag="ACHXOL_004218"
     CDS             complement(281..559)
                     /locus_tag="ACHXOL_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR6811024.1"
                     /translation="MAKVLNTYEQADFERLAAFYPYRNEHGLPVLEESLEDYAKRTNQ
                     SILAVRRQADRSVIPVTQEGRNTKRKVNLFAIFLKTIRNAEKYVQMTK"
     gene            655..966
                     /locus_tag="ACHXOL_004219"
     CDS             655..966
                     /locus_tag="ACHXOL_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR6811025.1"
                     /translation="MSIEISKKLKAIRESEGLSQAKFADSIGIAVGTVKQYETGIRGV
                     GTEVLLKITMHPEFKKYTTWLMSNETNEAAGQISPSLSPDGPENTSSSQKSRKTGTQP
                     G"
     gene            1055..>1973
                     /locus_tag="ACHXOL_004220"
     CDS             1055..>1973
                     /locus_tag="ACHXOL_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR6811026.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYSLVRDVIKTVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRF"
BASE COUNT          504 a          436 c          513 g          520 t
ORIGIN      
        1 gtgaaatcct gcccgaactg attgtcgcca cagctcagtg caaaaatgcg gtttccggta
       61 aacggattgc gtgggcattt gtggatcacg attccagctt tctcaatcag ccaggcgtgc
      121 tcgccgattt gttttactgg gtagccatcc ggcgttgcgt gtgtatcact caggctgtag
      181 cggatgttgc tgcgtgatgc actggtagtg aaacggttag cgtggcgttc tgttccggta
      241 caaaaattac ggcgttgctt cagcataaaa tgacacctcg ttattttgtc atctgcacgt
      301 atttctctgc gtttctgatg gttttcagga aaattgcgaa gagatttact ttgcgttttg
      361 tgtttcttcc ttcttgagta acgggaatta ccgatctatc agcctgcctt cttacagcga
      421 gaatgctttg atttgtgcgt ttcgcataat cctctaggct ttcttcaagt accggtagcc
      481 catgttcatt acggtatggg tagaacgccg ccaaacgctc aaaatccgct tgttcgtatg
      541 tgttaaggac ttttgccatg gtgtgataac ctattcaatc tggtgctatt tgtggctctt
      601 tgtagcgtca agtggtactc aactgataac caatatagta ttcaggtgca caccatgtca
      661 atagagatat caaagaagct aaaagcaatt cgagaatctg aggggcttag tcaggcaaag
      721 ttcgcggatt caataggtat tgcggttggt acggttaagc aatacgagac tggtattcga
      781 ggtgtgggaa cggaggtttt actgaaaatc acaatgcacc cggaatttaa aaaatacact
      841 acgtggttga tgagtaacga aacaaatgag gctgctgggc agatcagtcc ttctctctcc
      901 cctgatgggc cagaaaacac atcgtcttct caaaaatccc gcaagactgg cacacagccc
      961 ggctaatcat ggaacgctgg gggcatggtg gtcttgtaac gctggggctt cacgaatgag
     1021 cataaaatca attccgggag ggtatcttct tgacatgcgt cctgaggggc gtaaaggcaa
     1081 acgcattcgc aaaaaattta aaacgaaatc ggatgcagtt ttatatgagc ggtgggtgct
     1141 ggcgcaacag cataacaatg agtggaaagg aaactccatt gatcgccgtc cgctgtcagt
     1201 gcttattgac ttgtggtgga aataccacgg ccagctaatg aagtcagggc ataacacgcg
     1261 ccttaaattg ctgcgcttga gtgaggcaat ggatgacccg tgcgtgcata aacttaatac
     1321 aacgatgctc accgagctac gtgtgtccag gatagagcag gggatacagc ccagcaccat
     1381 aaatcgagag attggggcgt taagcgcgat gtttaccgca ctcatctcat ccggccattt
     1441 tcttaacgat aaccccgttc aaggccttaa aggaatgaag gttaacgagc gtgaaatggg
     1501 atatctgagt aagtctgaat gtgttcagtt gctggatgca ctggctgaaa atcccgatga
     1561 acggctggcc gtcgaaatcc ttctgtcgac cggggcgcga tggggcgagg tagcggcact
     1621 ggagcagcgc cgtgttcttc attgtcgaat cactttttca aaaacgaaga acagcaaaaa
     1681 ccgtaccgtt cctatttctg aaagcctgtt tgaaaagatc aaaaaacggg gcgggaaact
     1741 ggtgtttccg acgctggatt attcattggt tcgcgatgtc atcaaaacgg tcgcacctga
     1801 tgttcctgac ggccaggctg ttcatgcgct gcgccacacc ttcgccagtc atttcatgat
     1861 gaacggcggc aatattctga cgctccagaa aattctgggg cacgcaaaga ttcagacaac
     1921 gatgatttat gcccatcttg cgccggatta cttgcaggat gcggtgaggt tta
//
LOCUS       DAZRUF010000379         1966 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZRUF010000379 DAZRUF010000000
VERSION     DAZRUF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.382"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..314
                     /locus_tag="ACHXOL_004221"
     CDS             <1..314
                     /locus_tag="ACHXOL_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811027.1"
                     /translation="ALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYS
                     GAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQR
                     A"
     gene            416..1870
                     /gene="amn"
                     /locus_tag="ACHXOL_004222"
     CDS             416..1870
                     /gene="amn"
                     /locus_tag="ACHXOL_004222"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR6811028.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIDIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          471 a          490 c          469 g          536 t
ORIGIN      
        1 tggcgcttga agcacaatct attttctgga tagttttctt ctccataatg ctggcaaaca
       61 ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg tttggtgcca
      121 gttatcgcta tagtggcgct ggagtcggtt atcaggttgc cagtgtggtt ggcggtggat
      181 ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg catagcgtcg
      241 ccatttattt gctggctgga tgcctgattt ccgcaatgac cgctttgttg atgaaagaca
      301 gtcaacgcgc ttgatagcct ggcgaagatc atccgatctt cgccttacac ttttgtttca
      361 catttctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga cacacatgaa
      421 taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg cgctgtatga
      481 gcaatctgta gtcgcattac gcaacgccat tggcaactat attacaagtg gcgaattacc
      541 tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg taacctggga
      601 cggtagcaca accaatcccc ccaaaacgcg cgcatttggt cgttttactc acgcaggcag
      661 ctacaccacc acgattactc gccctactct ctttcgttcg tatcttaatg aacaacttac
      721 gttgctgtat caggattatg gtgcgcatat ctctgtgcaa ccctcgcagc atgaaatccc
      781 ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga gcgctgggtt
      841 aactcgctac ttcccgacaa cagaactggc gcaaattggc gatgaaactg cagacggcat
      901 ttatcatcca acggaattct ccccgctatc gcattttgat gcgcgccgcg tcgatttttc
      961 cctcgcacgg ttgcgccatt ataccggtac gccagttgaa cattttcagc cgttcgtctt
     1021 gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca gccagatcct
     1081 cgatcctgat agtccttaca ttgccctttc ttgtgctggc gggaactgga tcaccgccga
     1141 aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc agatgccagc
     1201 atggaattta attaccgccg atgctcaggg tattactctg gtgaatattg gcgtgggacc
     1261 atcaaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggatg tctggttgat
     1321 gattggtcac tgtggcggat tacgtgaaag tcaggccatt ggcgattatg tacttgcaca
     1381 cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata ttcctattcc
     1441 gagcattgct gaagtgcaac gtgcgcttta tgacgccacc aagctggtga gcggcaggcc
     1501 cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg gtaaccacag atgacaggaa
     1561 ctgggaatta cgttactcag cttctgcact tcgttttaac ttaagccggg ccgtagcaat
     1621 tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc catacgggac
     1681 actactgtgt gtttcagata aaccattaca tggtgagatt aaacttcccg gtcaggctaa
     1741 ccgtttttat gaaggcgcta tttccgaaca tctgcaaatt gacattcggg cgatcgattt
     1801 gctgcgcgca gaaggcgacc gactgcattc gcgtaaatta cgaaccttta atgagccgcc
     1861 gttccgataa taagttctga accttcttat cagaaataga tgtgaaggag taagtaagac
     1921 acctggcaaa tagcctgcaa taatcgtggg ctgtttgctt ccttgg
//
LOCUS       DAZRUF010000380         1945 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZRUF010000380 DAZRUF010000000
VERSION     DAZRUF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.383"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(37..957)
                     /locus_tag="ACHXOL_004223"
     CDS             complement(37..957)
                     /locus_tag="ACHXOL_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR6811029.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1095..>1945
                     /gene="pgrR"
                     /locus_tag="ACHXOL_004224"
     CDS             1095..>1945
                     /gene="pgrR"
                     /locus_tag="ACHXOL_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR6811030.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGS"
BASE COUNT          485 a          445 c          462 g          553 t
ORIGIN      
        1 aggccatatc actattgcgg atttgaatat gtttcattac agagtttcct caaagaacgg
       61 agctaatact gaaattgctt cagccacata ggctttgccg tcataaagat ccatatggtt
      121 tgcgccttca acaatgtgat agcgtttatc ttggcttgat gctcgatcgt acaggtcgtc
      181 actcatccat ttgctccctg cctggctgcc cgccacaatc tgcatcggct gagtcaggta
      241 cacttccgcc atatggtaag catcaaaggt aataatctgg ttaaggctgc gcaaagtagc
      301 gtaacccggt gctgttggat actgcgcgcg aggggtgtgg taatactccc aggcctgacg
      361 cagttcttcg ttcggcgcat cggactcctt cattggtgcc agtggcataa cggcatattc
      421 tccgctgcta atatcgctgg ttcgggcgtt tgaacccgct tcaacgtatg gcagggcatc
      481 aatagatttc acattgtttt cccaaccatt acggaacatc gaaccaatat tgaccgcact
      541 aacggtaccg atggccttga tgcggcgatc ctgaattgca gcattggctg tatatcctgc
      601 accggcacaa attcccatcg caccaattcg ggtattgtcg acataagaaa gcgttgtcag
      661 gtaatcaatc acggcactga tgtcttcagt acgaatgtat gggttttcta actgacgcgg
      721 ctcgccgcca ctttcacctt gataagatgc gtcataagca atagtgacaa atcccttttc
      781 cgccagtttt ttggcatagg ttccggccgt ttgttcttta acgcccccac ctgggtgaga
      841 taccacaatt gcctgatact gacgggtttc atcaaatttt ggagggaaat agatcactgc
      901 agacaaagag atggttggat tattgctgtt agtgaagctg actttattat tcatcattcc
      961 gttcctcatg gagttgtcgg ttcgttttaa cggttggtga tatcactata gatattgatc
     1021 attaagttga ttagacccta aattatgatt agacctatca tttaaatgat taatagattg
     1081 catggagata ctgaatgaag cgtgaagaaa tcgctgatct gatggcgttt gtcgtcgttg
     1141 cagaggagcg tagcttcact cgtgcagcag cccgcctgag catggcgcag tcagctttaa
     1201 gccagatagt gcgtcgtata gaagaacgat tgggattgcg gcttctgacg cgaaccacgc
     1261 gcagcgttgt tccaactgaa gcgggcgagc atcttttgtc tgttcttggc cctatgttgc
     1321 atgacataga ttcagccctg gcatccctga gcgagctgca gaaccgccca tccgggacaa
     1381 tacgtattac tactgtagaa catgcagcca aaacgatatt gttaccagca atgcgcacat
     1441 tcctgaaatc gcatcctgaa attgatattc agctcaccat tgattatggt ttgaccgatg
     1501 tcgtttctga acgttttgat gcaggcgtcc gtctgggtgg ggagatggat aaagatatga
     1561 tcgccattcg aatcgggcca gatataccaa tggctattgt tggctcaccg gattattttt
     1621 ctcgccgaag tgttccaacg tcagtgtcac aattaataga tcatcaggca attaatttgt
     1681 atcttcccac atcgggtaca gcaaatcgct ggagattaat acgcggtgga cgtgaagttc
     1741 gtgttcgcat ggaaggtcag cttttactga atacgataga cctgatcatt gatgctgcaa
     1801 ttgatgggca tggattggcg tatctacctt atgatcaggt tgagcgggct attaaagaaa
     1861 aaaaactgat acgtgttttg gataaattca caccagattt acccggttat cacctgtact
     1921 atccacaccg tcgacatgct ggctc
//
LOCUS       DAZRUF010000381         1921 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZRUF010000381 DAZRUF010000000
VERSION     DAZRUF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.384"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..583)
                     /gene="yeeO"
                     /locus_tag="ACHXOL_004225"
     CDS             complement(<1..583)
                     /gene="yeeO"
                     /locus_tag="ACHXOL_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR6811031.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPL"
     gene            744..819
                     /locus_tag="ACHXOL_004226"
     tRNA            744..819
                     /locus_tag="ACHXOL_004226"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:777..779,aa:Asn,seq:gtt)
     gene            complement(857..1807)
                     /gene="cbl"
                     /locus_tag="ACHXOL_004227"
     CDS             complement(857..1807)
                     /gene="cbl"
                     /locus_tag="ACHXOL_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR6811032.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          540 a          498 c          417 g          466 t
ORIGIN      
        1 atagcggtat tttcgtattc cctgcaccac gaagtgcccc gctaccaata agagtgatgg
       61 cagctgctgg ataactgagt accgtcagct ccagataagt caacgccagt gctttaactt
      121 ctgtcgtggc atcacccgcg acgaaatcaa taatttgttc gccaaaatga tgaataagcg
      181 ttgccaacag tacggcaaac aacgtcatga tcaccaatga ctgccgcgtc gccaccctcg
      241 ctcgtcgtcg atcccgctta ccgagactaa atgccacaac gacagtagta ccaagatcga
      301 tagcagcaaa aaaagccata atgaccatat tgaagctgtc cgccaatccc acgccggcca
      361 tcgcatcttt tcccagccag ctgaccagaa aagtgctcag aacccccatc aacaggacac
      421 aggcattctc catgaagata ggaacagcaa gcggggttat ctcgcgccag aacaacactt
      481 tgtagctctt gcgtttagcg tgccagcgag tgccgtgaac aacctggcgt aaagcagagg
      541 agatattcaa agccgacctt aattgcagaa agtgaaacca catttcaaat aatgagggag
      601 aatcagcaaa gctgcaaaga ttttcgccaa caaattgtct gcaaatgcaa caaactgttg
      661 atagaaacgg caaacagttg gggaatttaa aaatcgggtt tgacaaaaga tttttcgccg
      721 ttaagatgtg cctcaacagc gattcctctg tagttcagtc ggtagaacgg cggactgtta
      781 atccgtatgt cactggttcg agtccagtca gaggagccaa atttctgaaa agcccgcttt
      841 tatagcgggc tttttgctat atctgataat caatttcctc ttcactgttt tccatcacct
      901 gccgcttgat atcctcaact gacagtcctg cattacaaag ttccagaaag cgccagacat
      961 agttacgctg aagttgtcct cgcttcagtc ccaaccagac agtattagca tcgaaaagat
     1021 gccgcgtatc cagacggatt aaattctctt cctcttgttc gccgctggat tgttcggcaa
     1081 ctaatccgat cccaagccca agagcaacat aggttttaat gacatcagaa tcctgcgcac
     1141 ttaatacaat atctgccagc aaacctttgc gggcaaatgc gtcatcaata cgtgagcgcc
     1201 ccgtaatccc ctgtcggtaa gtgattaacg gccacttcgc tattgattcc agcgtcaatg
     1261 gtgtaatttg cgttaaggga tgatcgagtg gaacaagcaa actatggtgc caacgaaacc
     1321 acgggaaggc gacgagctgc gggtcattac tcaaacgctc gctggcgata ccaatatcag
     1381 cttcgccatt ttgcaacaac gtcgcaattt cctgtggcgt cccctggatt agctcgagcc
     1441 gaacctccgg gaaaagttcg cgaaaagctt taatgacctc tggcaagcta taacgtgcct
     1501 gagtatgcgt cgttgcaata gtgagaacgc cagacgtatc gttggtaaac aggtctgcaa
     1561 gccgacgaac attactggct tcattcagaa tacgttctgc aatgaccagt aatgctttgc
     1621 ccggttcagt catgcccagc agtcgcttac ctcgtcgaac aaatatttcg atgccaagtt
     1681 catcctccag ttcccgaata tgacggctga cgccggactg tgaggtaaaa agcatattcg
     1741 caacctctgt caggttgtaa tcctgacgtg cagcctcgcg gattatcttt agttgttgga
     1801 aattcacggt aaactccggg cagttcagat ttcccgttat tgttaaagtc taatgcctgg
     1861 cataacaaat aataaaaacc cgcatcttat tccatcccga tataacgctt agctcaccaa
     1921 t
//
LOCUS       DAZRUF010000382         1861 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZRUF010000382 DAZRUF010000000
VERSION     DAZRUF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.385"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..1105
                     /gene="ompN"
                     /locus_tag="ACHXOL_004228"
     CDS             <1..1105
                     /gene="ompN"
                     /locus_tag="ACHXOL_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR6811033.1"
                     /translation="PALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYFSDNSAKDGDQ
                     SYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGLKFADYGSFDY
                     GRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFFGLVNGLNFAV
                     QYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAYTSSDRTNDQV
                     NHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVANKTQNFEVTAQ
                     YQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATYYFNKNMSTYV
                     DYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1246..1680
                     /gene="uspF"
                     /locus_tag="ACHXOL_004229"
     CDS             1246..1680
                     /gene="uspF"
                     /locus_tag="ACHXOL_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR6811034.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT          489 a          439 c          428 g          505 t
ORIGIN      
        1 tcctgccctg ctcgccgcag gtgctgcaca tgcagccgaa gtttataata aagacggcaa
       61 caaattagat ctgtatggca aagttgatgg cctgcattat ttttctgata attcagcgaa
      121 agatggcgat cagagctatg cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca
      181 actcactggc tacggtcaat gggaatacaa tattcaggca aacaacactg aatcttcaaa
      241 aaaccagtca tggacccgtc tggcatttgc cgggctgaaa tttgcagatt acggttcttt
      301 cgattacgga cgtaattatg gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc
      361 tgaatttggc ggtgactctt ataccaatgc agacaacttt atgactggtc gtgccaacgg
      421 cgtcgcgact tatcgtaata ctgatttctt cggtctggta aatggtctga acttcgcggt
      481 gcagtatcaa ggtaacaacg aaggtgccag taatggtcag gaaggcacca acaacggacg
      541 tgatgttcgc catgaaaatg gtgacggctg gggtctttcc acaacatatg atttaggcat
      601 gggctttagc gctggtgcgg catacacctc atctgaccgc accaatgacc aggttaacca
      661 tactgcggcg ggtggtgata aagcagacgc gtggactgct gggctaaaat acgatgctaa
      721 caatatttac ctggcaacca tgtattcaga aacgcgtaat atgaccccgt ttggcgacag
      781 cgattatgct gttgcaaaca aaacccagaa ctttgaagtc actgcacagt accagtttga
      841 ttttggtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc tgcacgctgc
      901 cgatggtgca gacaacccgg caggtgttga tgataaagat cttgttaaat acgccgatgt
      961 tggcgcgact tactatttca ataaaaacat gtccacttat gttgactata aaatcaacct
     1021 gttggatgaa gatgacagtt tctacgctgc caacggcatc tctaccgatg atattgtcgc
     1081 tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg gctttttctg
     1141 cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac acaaaaagtg
     1201 tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa cagaacgatt
     1261 cttgtcccta tcgatatttc cgattcagaa ttaactcaac gcgtgattag ccacgttgag
     1321 gcagaggcaa agattgacga tgcagaggtt catttcctga cggtaatacc ttcactgcct
     1381 tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga tgacctgaaa
     1441 gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc aaccgacaga
     1501 gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt ggcgaagaag
     1561 atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac ttatctgctc
     1621 ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt tgtgcgctga
     1681 cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt gctgacattt
     1741 tcccctctaa tccgtaccat acacgccaca gttttatatc agatttctta tgctgggcgt
     1801 tccggcatga cgcatactct tctgatgcca tataacgaat tgagtcgctt ttaaatgtcg
     1861 c
//
LOCUS       DAZRUF010000383         1836 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZRUF010000383 DAZRUF010000000
VERSION     DAZRUF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.386"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(75..1583)
                     /gene="putP"
                     /locus_tag="ACHXOL_004230"
     CDS             complement(75..1583)
                     /gene="putP"
                     /locus_tag="ACHXOL_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR6811035.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
BASE COUNT          512 a          550 c          406 g          368 t
ORIGIN      
        1 tttatatggc gaggcacatt aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc
       61 gcaggctaag tcccttagtt ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca
      121 tcggcctcgg caaagcgttt ttgcatcgcc gctgacggcg ctttacccag caaactaaac
      181 actacaatcc cgatactgcc gaagatgaag cccggaataa tttcgtacag tcccagccag
      241 ccgaactgct tccagacgat aactgtcagc gcaccgatga tcatccccgc cagcgcaccg
      301 ttgcgcgtca tgcgtgacca catcaccgag aacagcacca ccggaccaaa cgccgcgcca
      361 aagcctgccc acgcgtagct cactaagccc agcacgcggt tttccgggtt tgccgccagc
      421 gcaatcgcca ccagcgccac caccagcacc atcacacgtc ctacccacac cagctctttc
      481 tggctggcct gtttacgcag aaacgctttg tataaatctt cggtaatcgc actggagcac
      541 accagcagct ggcaacttaa ggttgacatt accgccgcca gaatcgccga cagcagaatc
      601 ccggcaatcc acgggttaaa cagaatttgc gccagttcga taaacacgcg ttcagcgttc
      661 tggtttaccg cacctgctac cgccggatgc tcgttaaagt aagcgatacc aaagaagcca
      721 acagccaccg cccctgccag gcaaaggatc atccaggtca tactgatacg acgcgcatgg
      781 acaatgctgt ggtgagaatc tgccgccata aaacgcgcca ggatgtgcgg ctgcccgaag
      841 taacccagcc cccaacccat caatgagata atggcaacaa agttcagccc tttgagcata
      901 tccacgtttt cgatgctctt ttgtttgatc acttccagcg agtcaccaaa gccaccgaca
      961 ctgataatga cgataaccgg cgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca
     1021 gtgtcagtcc agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat cgtcgacgca
     1081 gctcccgcca acagagccgt ttcgtaactc atgccaaagg tactttcaaa cagacgcgcg
     1141 cctgccacaa tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac cagcgcggag
     1201 ataatgcgca aaatgcggct tttatcttca aaacgcccgg tgaaataatc cggcaatgtt
     1261 aaggcgttat tattgtattc ggtatgcaca cgcaaccgcc cggccaccag cttccagttg
     1321 atccacgcgc ctaatgtcag gccaatggcg atccagcttt cggaaatccc ggaaagaaaa
     1381 accgcgccag gcaaccccat taacagccag ccgctcatat ccgacgcacc cgccgataat
     1441 gccgtcacga atggcccaag actacgaccg cccagaatat agtcgtcaaa gtttttcgtt
     1501 gatcgccagg cgataaaccc aatcaatatc atgccaaaga tatagacaca aaatgtcacc
     1561 aacatcggtg tgctaatagc catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa
     1621 acttgccgtt atatctgcca ccggaacggg gtaacagagt ttatgtttta ccagggcgac
     1681 cgtatcctgc cggaagcgct ggttattcac aatcgattta acacaccatt tacattaaat
     1741 tttagtgctc agcgacacta tttttcatca ggttgcactc tctcacattt tttgcggttg
     1801 cacctttcaa aaaggttaac tgccgcagag aaaaag
//
LOCUS       DAZRUF010000384         1834 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZRUF010000384 DAZRUF010000000
VERSION     DAZRUF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.387"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            149..652
                     /locus_tag="ACHXOL_004231"
     CDS             149..652
                     /locus_tag="ACHXOL_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR6811036.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(731..982)
                     /gene="yecJ"
                     /locus_tag="ACHXOL_004232"
     CDS             complement(731..982)
                     /gene="yecJ"
                     /locus_tag="ACHXOL_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR6811037.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1097..1183)
                     /gene="azuC"
                     /locus_tag="ACHXOL_004233"
     CDS             complement(1097..1183)
                     /gene="azuC"
                     /locus_tag="ACHXOL_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR6811038.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1446..1769
                     /locus_tag="ACHXOL_004234"
     CDS             1446..1769
                     /locus_tag="ACHXOL_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR6811039.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          475 a          433 c          408 g          518 t
ORIGIN      
        1 tcagacgtta ttcttatttc agatcatcgt cagaattgac tccacgatca catttcggac
       61 cggcagaaag gaattattct gcaaacagta attatggtgt tttgatttat cttgcacctc
      121 tccacttctg gatataagga tattaggtat ggcaaccgct ggaatgcttc tcaaactcaa
      181 ctctcaaatg aaccgcgagt tttacgcatc caatctctac cttcacctga gtaactggtg
      241 ttctgaacag agtctgaacg gcaccgccac tttccttcgc gcccaggcac agagtaatgt
      301 gacccaaatg atgcgcatgt ttaactttat gaagagtgtc ggcgctaccc ccatcgttaa
      361 agccattgat gttcccggtg aaaaactgaa ctctctggaa gaactgttcc aaaaaacgat
      421 ggaagaatac gagcaacgtt ctagtacgtt ggcacagtta gccgatgaag cgaaagaact
      481 gaatgatgat tcaaccgtca atttcctgcg cgatctggaa aaagaacagc agcatgatgg
      541 tctgttgctg caaaccattc ttgatgaagt gcgcagtgcg aaacttgcgg gtatgtgccc
      601 tgtgcagacc gaccaacatg ttctgaatgt cgtgtcacac cagctgcatt gatcatcatc
      661 ggcgctaatg cattgcgccg atgaaggttt tgagaaaccg ctgcctcatc tgtttgaagc
      721 agcggttttt ttaatgggat tcaccctgtg gggtaaactt gagttcaata agcgcgatgg
      781 ctttttggat tgcccgcatg gtgaccgggt ctgcggcggc gggatggtta gtaaagtcga
      841 tattcttcag ctgactggac attttttcac gaacttcaac gggcgcgatt acatcgagaa
      901 catccagaat ttgtttgata accaactggc aagcaaccac atcagaaacc agttcctgat
      961 cggcattcag cggctgggac atcgtaaact cctgatagca ttttgaaagc cgttatagta
     1021 gcggcttcac atcttcagcg atagtcacat ccaccgtcat caggacacaa aaaaacctgc
     1081 cggagcaggt tttttgttat cggaacatat tgcctggcgg tacgtctttg aacgtcttgc
     1141 aatagttatt gaacatactt ttcaggattt tgcgcagttt catcgcggca ctccgaccat
     1201 ttgttataca ggtgttattg tctttgcgct tataatatga caaccatcac aaaaatcaat
     1261 ctttatgtga tacaaatcac ataaataccc ctttaatgtt ataaaaatga taatcaaaaa
     1321 acagcccccc tatttctgac acctacagat ggcaagaaat agcgcctgcc aggcgtcttt
     1381 tccggccatt gtcgcagcgc tgtaacgcgt aaaatagtgc tttctcttac tcttctggct
     1441 ggaccatgag acttctgatt ctgactcttt cattaataac gctggcgggg tgtacggtta
     1501 ctcgccaagc ccatgtgagc gaagttgacg cggcaacagg tattgtacgg ttggtttatg
     1561 accaggcttt tttgcagcat gcccataccg atcgttatgt cagtcgcggg atcgccgatc
     1621 gcgcatgcca gcaggaaggc tatacccacg cggtcccctt tggtcagcca gtaggcaact
     1681 gtagcctttt tgcgggttcc ttatgtttga atacggaatt cactttgtca tatcagtgcc
     1741 atcattccgc cttccctgtc tttctctaaa tattttaatc agcgagggga tcttcgctga
     1801 ttaaagaaat agcggtaatg cgttttattc ccat
//
LOCUS       DAZRUF010000385         1804 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZRUF010000385 DAZRUF010000000
VERSION     DAZRUF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.388"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHXOL_004235"
     CDS             37..264
                     /locus_tag="ACHXOL_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR6811040.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHXOL_004236"
     CDS             complement(265..639)
                     /locus_tag="ACHXOL_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR6811041.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHXOL_004237"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHXOL_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
BASE COUNT          447 a          461 c          452 g          444 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtga
//
LOCUS       DAZRUF010000386         1772 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZRUF010000386 DAZRUF010000000
VERSION     DAZRUF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.389"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(122..1072)
                     /gene="corA"
                     /locus_tag="ACHXOL_004238"
     CDS             complement(122..1072)
                     /gene="corA"
                     /locus_tag="ACHXOL_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR6811042.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1224..1280)
                     /gene="ysgD"
                     /locus_tag="ACHXOL_004239"
     CDS             complement(1224..1280)
                     /gene="ysgD"
                     /locus_tag="ACHXOL_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR6811043.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1442..>1772
                     /locus_tag="ACHXOL_004240"
     CDS             1442..>1772
                     /locus_tag="ACHXOL_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811044.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          477 a          444 c          436 g          415 t
ORIGIN      
        1 ttactgagta tcaattaaca caagaaaata aaatagagcg agttttcaat ccgcatgact
       61 ttaaataaga caatttttag aaataagccg gataaaaaaa tagctttatc cggcactcat
      121 attacagcca gttcttgcgc ttaaagtaca gatacggtgc caggcccgcg aggatcataa
      181 agataatcgc gcctgggtag ccgaaactcc acttcagttc tggcataaac tcaaagttca
      241 tcccatagct ggaagcaacg agcgtcggcg gcaggaatac cacggatacc accgagaaga
      301 ttttgatgat gcggttctgc tcgatgttga taaaacccat cgccgcctgc atcaggaagt
      361 tcaccttctg gaacagggat tcgttatgcg gcagcaggga ttcgatatct cgcaggattt
      421 cacgcgcctg ctccagttgc ccacccggta aacgcgcttt acgcaccagg aagttgagcg
      481 cgcgctgggt atccatcaga cacaggcgaa ctttccagcc gatatcttcc agttccgcca
      541 gagtggagag cgcctcgtcg tactcatcgc cctgatgccc ttccataatc acccggctca
      601 actgctccag gtcgctatag atattttcaa tttcatctgc caactgttcg attttggttt
      661 cgaacagatc cagcagcagc tcgtaggcgt taccgtctac catcgactgg ctacgggcac
      721 gcatacgata cagacgaaaa gcgggcagtt cacgctcacg cagagtaaac agacgaccat
      781 cacggatggt aaatgccaca gtggagttac cggcgtgatc ttccgcatct tcaaagaaga
      841 agaaggagtg aatatgcagg ccgtcgtcgt cttcaaagaa acgtgccgat gcttcgatgt
      901 cttccagttc cgggcgggtt gccaggctct ggccaagttc agattgtacg cgcagtcgct
      961 cgtcgtcgtc cggttcgaca agatcaatcc atactgcatt tacaaggggt tgtgactctt
     1021 cgacttccag ccgggtcagt cggttatttt ccagttgaaa tgcgctcagc atgaccggga
     1081 ctcccaatgc ttaaaatatc ggacagttcg gtgggcacac agaaacaaat tgggtttcag
     1141 accattaaac agcctgactc agcgcgacgg gaaaaattga ggtcgctgac aaccgctaag
     1201 gctatcagca aaaagggata gccttaggag ttgatcctgg atgacaggat agtgagccag
     1261 tatctactgg gtgtgtccaa ggcgaatgtc ctcttagagt gatcgtgcgc gcatgttacg
     1321 ccagcaaaat tttgccgtca acacgcaaca gaacaccaca gaacaatagt tgaacttcgc
     1381 gctttttggt actcgtcaag gtcgctaaca ggataaaaaa atccaacatc caggacctat
     1441 aatggcgcat cggctactca ttggtaaagg aatgatcacc ttgaatctca aacggatttt
     1501 tctcgccctc accttacttc ccttgtttgc tgttgccgct gatgattgcg cactctcaga
     1561 tccgatgctg accgtacagg cgtataccgt taatcctcaa acagagcggg tgaaaatgta
     1621 ctggcaaaaa gccaatggcg aagcgtgggg aactttacat gctctgctgg tggatatgaa
     1681 tagtcagggt caggtgcaga tggcgatgaa cggcggcatc tatgatgaaa gctatgcgcc
     1741 gctcggtttg tacatcgaaa acggtcagca ga
//
LOCUS       DAZRUF010000387         1771 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZRUF010000387 DAZRUF010000000
VERSION     DAZRUF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.390"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..875
                     /locus_tag="ACHXOL_004241"
     CDS             <1..875
                     /locus_tag="ACHXOL_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR6811045.1"
                     /translation="RKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYDL
                     HSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEMLT
                     SEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNAN
                     IGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQRD
                     RYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVDH
                     ADYVWFVAPDTTKR"
     gene            883..1659
                     /locus_tag="ACHXOL_004242"
     CDS             883..1659
                     /locus_tag="ACHXOL_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR6811046.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT          516 a          406 c          407 g          442 t
ORIGIN      
        1 tgagaaagtt catccttatt tctatgataa cgttagctag tgttagtctg ggagcctgca
       61 gacagaatgt caccataaag caggatgcgc cagggcagaa agctttcctg actgacgggc
      121 agatttacga tttgcacagt ggcaaaatta tttctagcag cgagcttttg gcggatcttg
      181 ctaccgctcg acatcttatt attggtgaaa aacacgataa tgccgagcat catcagattg
      241 agctttggct tattcaaaat ttgctcatcc agcgaccaca ggggagtgtg ttgcttgaga
      301 tgttgacgtc agagcaacag ccccgagtta accaagttaa atgttggctg aaagataatc
      361 ctgtggttcg agatagccgt gttcaggaac tacttaactg gcaaaaaggg tggtcctggg
      421 agatgtatgg cgacatcgtc atgcagctat tacgtgggcc ttacccgctg ctcaatgcca
      481 acattggccg ggagcagatg ttggcactgt acaaaaaaaa cgaatttcct aaaggaaaaa
      541 aatccactgc acctgtggta caagaagctc tgcgtgaaac catcatatct atgcatgaag
      601 gcaaccttga aagccagcaa ctgacgtcaa tgctctctat tcagcagcag cgggatcgct
      661 acatggcccg ccagcttctg agtgccccgg taccgtcgtt gttgattgca ggtggttatc
      721 acgcgtcaaa aagcatgggc gtaccgttac acatggaaga tcttgccacc ggcacccatc
      781 cagtggtgtt gatgcttgca gaaaaaggaa tgaacatcac agtagatcat gctgactacg
      841 tgtggtttgt agcgccagat acaacaaaaa ggtaaaccat ggatgatgaa tattctccac
      901 ttctcacagt cggtaaaatg gagcagctgg tttatctgtt ctctactgct tcatggactg
      961 attttccttg cgtttatctg gcgtttttcc gaagttcagc ccgctatgtc tcctgcaccg
     1021 gcgatcatgc tgcaatgggc agaaattata gaggctcctt cttctccact ttcgcttccg
     1081 gtgggaatcg ctcaacagga gtccgcagtg acagaagaaa agcaacaaac tgaggacaga
     1141 caacaacgtc ctgtcactga agacagtgat gcaaccattg aaataactag gaagaaaaag
     1201 tcatccgatg gagaaaagaa aaagacacgt ccacctcgta agataaaggc tcaaaccagc
     1261 gacagcaacc ccacagccgt ttccagtaat gctgcacctc aggctctggt cgaatcgtcc
     1321 cgtattgctg cgccttttaa cagtgactca acaaagcgag ataattctga ggcatcttgg
     1381 gaaagcagag taaaaggcca cctaaaccgt tataaacgtt accctggcga tgccagaaaa
     1441 cgtgcacgaa ccggcacagc cgtcgtgact tttaccgtaa acacggaagg cactattgtc
     1501 agtagtttct tggaaatctc ttcaggaacc ctttctcttg accgcgaagc tattgctgtt
     1561 ttagaacgcg ctcaaccact tcccaaacca ccgcctgaaa tattggaggg aggattattt
     1621 aaagtaaaaa tgccgattac ctttaagttg aaggagtaaa gggataattg gccgttcaag
     1681 ttcatttaac tgaaatttaa ttttcatttt tatgctgaac attttatcaa acttttccac
     1741 tgtcgaaaaa atcaaaaaga cagtagaaca t
//
LOCUS       DAZRUF010000388         1770 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZRUF010000388 DAZRUF010000000
VERSION     DAZRUF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.391"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            113..307
                     /locus_tag="ACHXOL_004243"
     CDS             113..307
                     /locus_tag="ACHXOL_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811047.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            315..1727
                     /gene="pykF"
                     /locus_tag="ACHXOL_004244"
     CDS             315..1727
                     /gene="pykF"
                     /locus_tag="ACHXOL_004244"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR6811048.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          477 a          437 c          421 g          435 t
ORIGIN      
        1 caatttattg tctaacaagt tgtatatttt ttgaaacgct gtttttgttt tccttttgga
       61 ttaatttcag cgtataatgc gcgccaattg actcttgaat ggtttcagca ctttggactg
      121 tagaactcaa cgactcaaaa acaggcactc acgttgggct gagacacaag cacacattcc
      181 tctgcacgct ttttcgatgt cacctatcct tagagcaagg caccaccact ttcgtaatac
      241 cggattcgct ttccggcagt gcgcccagaa agcaagtttc tcccatcctt ctcaacttaa
      301 agactaagac tgtcatgaaa aagaccaaaa ttgtttgcac catcggaccg aaaaccgaat
      361 ctgaagagat gttagctaaa atgctggacg ctggcatgaa cgttatgcgt ctgaacttct
      421 ctcatggtga ctatgcagaa cacggtcagc gcattcagaa tctgcgcaac gtgatgagca
      481 aaactggtaa aaccgccgct atcctgcttg ataccaaagg tccggaaatc cgcaccatga
      541 aactggaagg cggtaacgac gtttctctga aagcgggtca gacctttact ttcaccactg
      601 ataaatctgt tatcggcaac agcgaaatgg ttgcggtaac gtatgaaggt ttcactactg
      661 acctgtctgt tggcaacacc gtactggttg acgatggtct gatcggtatg gaagttactg
      721 ccattgaagg taacaaagtt atctgtaaag tgctgaacaa cggcgacctg ggcgaaaaca
      781 aaggtgtgaa cctgcctggc gtttccattg ccctgccagc actggctgaa aaagacaaac
      841 aggacctgat ctttggttgc gaacaaggcg tagactttgt tgctgcttcc tttattcgta
      901 agcgttctga cgttatcgaa atccgtgagc acctgaaagc gcacggcggc gaaaacatcc
      961 acatcatctc caaaatcgaa aaccaggaag gcctcaacaa cttcgacgaa atcctcgaag
     1021 cctctgacgg catcatggtt gcgcgtggcg acctgggtgt agaaattccg gtagaagaag
     1081 ttatcttcgc ccagaagatg atgatcgaaa aatgtatccg tgcacgtaaa gtcgttatca
     1141 ctgcgaccca gatgctggat tccatgatca aaaacccacg cccgactcgc gcagaagccg
     1201 gtgacgttgc aaacgccatc ctcgacggta ctgacgcagt gatgctgtct ggtgaatccg
     1261 caaaaggtaa atacccgctg gaagcggttt ctatcatggc gaccatctgc gaacgtaccg
     1321 accgcgtgat gaacagccgt ctcgagttca acaatgacaa ccgtaaactg cgcattaccg
     1381 aagcggtatg ccgtggtgcc gttgaaactg ctgaaaaact ggatgctccg ctgatcgtgg
     1441 ttgctaccca gggcggtaaa tctgctcgcg cagtacgtaa atacttcccg gatgccacca
     1501 tactggcact gaccaccaac gaaaaaacgg ctcatcagtt ggtactgagc aaaggcgttg
     1561 tgccgcagct ggttaaagag atcacttcta ctgatgattt ctaccgtctg ggtaaagaac
     1621 tggctctgca gagcggtctg gcacacaaag gtgacgtcgt agttatggtt tctggtgcac
     1681 tggtaccgag cggcactact aacaccgcat ctgttcacgt cctgtaatat tgcttttgtg
     1741 aattaatttg tatatcgaag cgccctgatg
//
LOCUS       DAZRUF010000389         1768 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZRUF010000389 DAZRUF010000000
VERSION     DAZRUF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.392"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..256
                     /locus_tag="ACHXOL_004245"
     CDS             <1..256
                     /locus_tag="ACHXOL_004245"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR6811049.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            315..>1768
                     /gene="dtpC"
                     /locus_tag="ACHXOL_004246"
     CDS             315..>1768
                     /gene="dtpC"
                     /locus_tag="ACHXOL_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR6811050.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFTIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESN"
BASE COUNT          371 a          428 c          471 g          498 t
ORIGIN      
        1 gaccgaagaa gtttacctcg acgaaatggt aggtcgtatt aacgccaata tgatccttcc
       61 gtatccgccg ggagttcctc tggtaatgcc gggtgaaatg atcaccgaag aaagccgtcc
      121 ggttctggag ttcctgcaga tgctgtgtga aatcggcgct cactatccgg gatttgaaac
      181 cgatattcac ggtgcatacc gtcaggctga tggccgctat accgttaagg tattgaaaga
      241 agaaagcaaa aaataattag ctcgtacaag ggaagtggct tgccacttcc cttttttgct
      301 cataaggaga acacatgaaa acaccctcac agccgcgcgc gatatactat atcgtggcga
      361 tccaaatctg ggagtacttc agtttttacg gcatgcgtgc cttactcatt ctctatctca
      421 cccatcagct tggttttgat gataaccatg ccatcagcct gttcagcgca tatgcttctc
      481 tggtttacgt tacccctatt ctcggcggct ggcttgccga ccgcctgctc ggcaaccgca
      541 ctgcagtgat tgccggcgcg ctgttaatga cccttggcca tgtggtgctg ggcattgata
      601 caaattcaac ctttagcctg tatctggcgc tggcaatcat tatttgtggc tacggtttat
      661 tcaaatcaaa catcagctgt ttgcttggcg agctctacga cgagaacgat catcgacgtg
      721 atggcggttt ttcgctgctg tatgctgcgg gcaatatcgg ttctatcgcc gccccaatcg
      781 cctgcggcct ggctgctcag tggtatggat ggcatgttgg ctttgccctt gcgggtggcg
      841 gcatgtttat cggtttgttg attttcttaa gcggtcatcg tcatttccag tccacgcgta
      901 gtatggataa aaaagcgctc actagcgtca aatttgcctt accagtatgg agctggttag
      961 tggtgatgct ctgtttagcc ccagtatttt ttaccctgct gctggagaac gactggtccg
     1021 gatatttgct ggcgatcgtt tgcctcattg ccgcacaaat cattgcccgc atgatgatca
     1081 aattccccga acaccgccgt gctctttggc aaattgtatt gttgatgttt gtcgggacgt
     1141 tgttctgggt actggcacaa cagggcggca gtaccatcag cttgtttatc gatcgctttg
     1201 tgaatcgtca ggcattcact attgaagtac ctacagcact attccagtcg gtgaatgcaa
     1261 ttgcagtgat gctcgctggg gttgtactgg cctggctggc gtcgccagaa agccgcggca
     1321 actcaacatt gcgcgtctgg ctgaagtttg cctttggctt actgctgatg gcttgtggct
     1381 ttatgttgtt ggcatttgat gcccgacatg cagcggctga cggtcaagcg tcaatgggcg
     1441 tgatgatatc cgggttggcg ctaatgggct ttgcagaact ctttattgac ccggtggcga
     1501 ttgcgcaaat cacgcgtctg aaaatgtctg gcgtattaac cggtatttat atgctggcaa
     1561 caggcgcggt cgccaactgg ctggcaggcg tcgtggcaca gcagacgaca gagtcgcaaa
     1621 ttagcggtat ggtaattgca gcttaccagc gattcttttc tcagatggga gagtggacgt
     1681 tggcttgtgt cgcgatcatc gtcgtattgg cctttgctac ccgttttctg tttagcacgc
     1741 cgacgaatat gatacaggag agcaacga
//
LOCUS       DAZRUF010000390         1746 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZRUF010000390 DAZRUF010000000
VERSION     DAZRUF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.393"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(26..330)
                     /locus_tag="ACHXOL_004247"
                     /pseudo
     CDS             complement(26..330)
                     /locus_tag="ACHXOL_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            428..688
                     /locus_tag="ACHXOL_004248"
     CDS             428..688
                     /locus_tag="ACHXOL_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811051.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            685..1275
                     /locus_tag="ACHXOL_004249"
     CDS             685..1275
                     /locus_tag="ACHXOL_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811052.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(1295..1642)
                     /locus_tag="ACHXOL_004250"
     CDS             complement(1295..1642)
                     /locus_tag="ACHXOL_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR6811053.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
BASE COUNT          407 a          487 c          489 g          363 t
ORIGIN      
        1 gcatcatttt ttccagaggc ggtcaaactc cgccttcgca aaataagcgg aagcctggcg
       61 aaggatatcg ttactgcggc gcagttcacg attttcacgc tccagctctt tcagacgctg
      121 acgttcagcg gtggtgagcc ctccatcacc gcccccggta tcccgctcat gctggcgaac
      181 ccggacacgc agagtctccg gcgtacagcc aatctttgga gcaatggaac aaattgtcgc
      241 ccattgtgag tcatattcgc cctgactttc cagaaccata cggactgccc gttgacggac
      301 ttcgggggaa aaacgagtat ttttagtcat cctgtttacc tctttctcag gaagtttagt
      361 ctccaggatt cccggggcgg ttcaatgatt tttacctgtc agaacattat cagatggaac
      421 gtgcaggatg gatcaggaaa tgacgttttc actcagctat gaacaactga cccggtttgc
      481 ggaaaaacgc atccgggaat gcaatctgga cagccacggc gtaacttatc tctgcgagtc
      541 agccaaagcc ggagccgtgc tgatcttctg gcatgaactg gccataaatg gctatacctc
      601 aatgaatgcc ataaagagac aggagatcat tgacgccgac catcagcgcc tcagaaagct
      661 catctggccg gaggatgact ggaaatgact cagagccgcc gaccgtcgcc actgcagcgg
      721 agagtgctga ttgtgctggc tgcccttgat gaaaaacggc caggacccgt gctgacgcgg
      781 gacatagaac gggtgctgga gcaaagcggg gaagccccgg tctacgggcc aaacctgcgc
      841 gcctcctgcc gtcgtctgga ggatgccggc tggctgcgta ccctgcgcgc tccgaacctg
      901 cagctggcag tggagctgac ggacgccggc cgggccgtgg cacagcctct gctgctggca
      961 gaacaggacc gcatccgggc agaacagcgt gcggcagaag tcgtcgtact gccgctggtc
     1021 ccggcagcag gtctgcccgc aggcggcact tctgccactg atctggcggt ggagctgaac
     1081 ggcataacgt atcaggcctg ccgtggggat tttgtggtgc gtctcgacgg cagcacctgt
     1141 ctgcagttat ggaataaaga aggctgggtg gtacgccggg agggcgatcc gctggaagtg
     1201 gcgcagtggc tgcaggcctg tcacgatgca ggaatggagg ttcgcgtaca gataaatgaa
     1261 agtgccgctc cgtaagaaca cgcctgtgca cggtttacac atcatcccat ggtggcagtc
     1321 ggttaacttt cctgcgccac cagtggaaag aggccatgag cagtccggac aaaaaacccc
     1381 ccgtgatact tataataatg gcctcacctg ccaccattcc tgacggcccc cagtagataa
     1441 accacattgc acatccccag gaaatgcccc ataagccccc cgtcagcacc gtgacctgcc
     1501 agaacggcat aaagggtaat ggcggaagcc ggatacccag ccgccagaga atacgcagaa
     1561 gaggaggggc ataattgctc cgccacatct ttttgctgtc catcagggca atggcccggg
     1621 cttttttctg ctcaaaagtc acaggagcac ctccgttcca tgatgtaaac gtgacacagc
     1681 ttattgttat tgattattat tatcatgctg tggttatgtt gtcatgcatc agtacactga
     1741 caaaag
//
LOCUS       DAZRUF010000391         1733 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZRUF010000391 DAZRUF010000000
VERSION     DAZRUF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.394"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..415)
                     /locus_tag="ACHXOL_004251"
     CDS             complement(<1..415)
                     /locus_tag="ACHXOL_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR6811054.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
     gene            complement(431..1105)
                     /locus_tag="ACHXOL_004252"
     CDS             complement(431..1105)
                     /locus_tag="ACHXOL_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR6811055.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(1185..1727)
                     /locus_tag="ACHXOL_004253"
     CDS             complement(1185..1727)
                     /locus_tag="ACHXOL_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6811056.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          492 a          386 c          313 g          542 t
ORIGIN      
        1 tttccagttc ttccacccag gcctgcggga cactgaaatc gagacgaaaa accccgatat
       61 cccaggtata gctcccaccc tgaacaagtt gctcaaatgt taaacattgc ttaccgctgg
      121 cgaagttatc gctattaatg cctaaccgct tgataacttc tcttgataaa catgtttctt
      181 gcgggttgtc tttaacaata atctcatatt tcccgcgcca ttgcttattg acataaatat
      241 cgatgtcata ctgccccggt aagggttgat tatcatcaag acgaatattt gcaacctgct
      301 ggtctttcat tccacctatc ataaaatggg tatcaaaggt ttcttcagct gcataagctt
      361 caatgccgag caataacgct acgattgctg atgcaagtgg ggtcattctc aacataggca
      421 gctcctgcaa ttaaattttg tcactaatat agttgccatg gtcatcgata atggtcagat
      481 gccagttatt tgcattatta cttttgacat taacactctg actttctaag ggggcaatca
      541 taatagtttc ataattgact ttgacattat tagctttgac atccgaaatc gtcacccaat
      601 tagctgagtc attttttatc accaaaccat tgccactgcg atttaccagc aattttttaa
      661 atgtcgcttt atttaccgga gcaatacccg ctggtcggta aaacaactta attctgtttt
      721 gcatcgcaaa cttcagtgca ttcttacctt cttgctctgg actatttggt ggaatatcca
      781 gaacattcag ataaaaaatg ctttctttat tagtgggcag tttattcggc ataattttga
      841 tttttacttg ctgcccggaa tttgccccta tttttgccac tggtggcgtt aacatgaaag
      901 gaacctgaat tttttctggt ggtaatgacg tatcgccatc atcaatccac gcctgcagca
      961 gcgaagaacg gtttccctgg ttcatcaact gcaccatcac ttctttattt tctgccgggt
     1021 aaataatgcg cgtcccgtag ataacaattc cggcatgtgc aggaaggacc atagaaaaaa
     1081 tgagtaaaga taataatcct ttcattttaa tgcctcttaa attaaaaggc cgccatggga
     1141 tagcggccat gtttattacc tggagaaact tacaatttta tttattaatc ctgcgccaca
     1201 cgcataacca gagaagataa cacatcacct gattgcactg agttatctgt cagtttcagg
     1261 tattttgctt taaacggaat agcatattga ccttccgctg ttttataact ctcaatgtta
     1321 aaaggtgcag aaacatccag gacatcgctg ttatcaccta aatagagata gtaacccacg
     1381 gtatcgttgc tactggctat cgccttatcg ttaccggtta ttttatcgtt ggtaatcaac
     1441 gagagtttta caccagcaac ttctttcggg cagctaattt ttaacatgct gctggttaca
     1501 ccgactgcat catcataggt agaatattta gctttacctt caaagacagt ggttggtaca
     1561 gtcggcatat taatacttac tgaatcacca cccgcttcga atttacacgg agattctgca
     1621 atattcccct taatagttaa tgttccggta tcaacgtctg cagcaaatac tccagcgctc
     1681 ataaaaaaag aagaaaagac agcggcagca ataattgaac gtttcataat atg
//
LOCUS       DAZRUF010000392         1732 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZRUF010000392 DAZRUF010000000
VERSION     DAZRUF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.395"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            49..1149
                     /gene="chaA"
                     /locus_tag="ACHXOL_004254"
     CDS             49..1149
                     /gene="chaA"
                     /locus_tag="ACHXOL_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR6811057.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1552..1659
                     /locus_tag="ACHXOL_004255"
     CDS             1552..1659
                     /locus_tag="ACHXOL_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR6811058.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT          403 a          413 c          436 g          480 t
ORIGIN      
        1 cctatttttt acattttcga taaattcatc tgcaaaggga taaccataat gtcaaatgct
       61 caagaggcgg taaaaacccg ccacaaggag acttcgctta ttttcccggt tctggcgctg
      121 gtagtgctgt tcctgtgggg aagcagccag acactaccag tggtcattgc catcaatctt
      181 cttgcgctta ttggtatttt aagtagcgcc tttagtgttg tccgtcatgc ggacgtatta
      241 gcccatcgcc tgggagaacc ttacggttcg cttattctta gcctttcagt ggttattctt
      301 gaagtcagtt tgatttcagc tttaatggca accggcgacg ccgcgccaac gctaatgcgt
      361 gatacgctct attcaatcat tatgattgtt accggtgggc tggttggctt ttcattattg
      421 ttgggcggtc gtaagtttgc cacccaatat atgaatctgt ttggtatcaa gcagtattta
      481 attgcgctgt tccccctggc gataatcgta ctggtatttc caatggctct gcctgcggcg
      541 aatttttcaa ccggtcaggc gttactggta gcattaattt ctgcggcaat gtatggcgta
      601 tttttgctga tccagaccaa aacgcatcaa agtttgtttg tctacgagca cgaagatgac
      661 agtgatgacg acgacccgca ccacggtaaa ccgtctgccc atagcagcgt gtggcatgct
      721 atctggttga ttatccatct gattgccgtt attgcagtca ccaaaatgaa tgccagcccg
      781 ctggagacat tgctcgacag catgaatgcc cctgtcgcct ttactggctt cctggtggct
      841 ctgttgattc tgtcgccgga aggtttaggt gcgttaaaag cagtgttgaa caaccaggtc
      901 cagcgcgcga tgaatctgtt ctttggttca gtgttagcaa ccatttcgct taccgtacct
      961 gtcgtcacgc taattgcctt tatgacgggt aacgaattac agtttgcact tggtgcgcca
     1021 gaaatggtgg tgatggtggc ctctttagtg ctgtgccata tctccttctc caccggacgt
     1081 actaacgtgc tcaatggcgc agcgcatctg gcactgtttg ccgcctattt gatgacgata
     1141 tttgcctgac agttatatct cctccgatga cggaaatcgg gggagataag ataaattcct
     1201 gcttattgta tttcttcata ctacctggac agctcgttaa tcagaattgc gagcctgtac
     1261 cacaacctgc acgaaaccac tgcaacagct gttgaaaagc cggaatgtac tctttattgc
     1321 aaacatttca gggatgaagt gaagatgaat tgagacgtag tatccacacc aagccggaat
     1381 tatccggtga ggcgcaatgt tgcgggggct ttatccctgg tggcattggt tgctggaaag
     1441 agaaaacccc cgcacgttga gaggtaaaac ctgacaacaa aacgggggct aatcttgaaa
     1501 ccagacactt caagaatagc cgccttgttg tcattacaac aaggcggcta tatgacgctc
     1561 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
     1621 accgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
     1681 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc gg
//
LOCUS       DAZRUF010000393         1728 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZRUF010000393 DAZRUF010000000
VERSION     DAZRUF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.396"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..356)
                     /locus_tag="ACHXOL_004256"
     CDS             complement(<1..356)
                     /locus_tag="ACHXOL_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR6811059.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLA"
     gene            complement(356..850)
                     /locus_tag="ACHXOL_004257"
     CDS             complement(356..850)
                     /locus_tag="ACHXOL_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFR6811060.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1208..1384
                     /locus_tag="ACHXOL_004258"
     CDS             1208..1384
                     /locus_tag="ACHXOL_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811061.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLIREYGD
                     DTAHDVSGLNTFIR"
     gene            complement(1526..1678)
                     /locus_tag="ACHXOL_004259"
     CDS             complement(1526..1678)
                     /locus_tag="ACHXOL_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811062.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            complement(1666..>1728)
                     /locus_tag="ACHXOL_004260"
     CDS             complement(1666..>1728)
                     /locus_tag="ACHXOL_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR6811063.1"
                     /translation="IRDQTALRTLQEYIRTQCLR"
BASE COUNT          441 a          476 c          399 g          412 t
ORIGIN      
        1 ctggcgagtc gttttttgga gtgttcgcgg gctttttcct ccgtcatctg aataatcagc
       61 atatcagcag gatcgcaaat cacgttgtaa atcacccagc cgtcaatcag gccgatagtc
      121 ttgccggttc gtgccgggcc aacaaatatc actgcgtcgt attcacgcga ggccaggcag
      181 ttcatcggct caataacata cggtgccacc agcggatccc acgggactga gttccctgcc
      241 cccatgggca cccgcatata ctgagcaacg gcatcagcaa cccgcattcg tctcggtgcg
      301 cgaaggatat aacctgaatc ggttcgtgct gcctttgcgg tttcctgatt cagcattact
      361 cctcctgctg taattcctcc tcatcatccg cacctgcttc agtcacccgc agggctatct
      421 gatcgcgcag atcatcaata atggactgaa cacggctcac agcggcaggc tgcagaccgc
      481 agtcacgttc aagaatatcc ggtaatgtct ccagcacctg cacgaccgct tttgcccaga
      541 tggcaaactc ccgtctgaca tcactggccg gaatgagttg tgccgtttcc tgttcgaact
      601 taagacgctc acgttcagac tgataccagg ctttgcgctc atgcgcgtcc atttcgcctt
      661 ctgcaaccgg cggtggtaat gccagaaatg ccgacacaat atcaaccacc cgataaagct
      721 tgaggttgct ttcatgcccc cctgcaacgg gtagattttg cagccttgcc gcagcagtct
      781 ggcgatgtac acctgacagt gccgccagtt gactgatatt cagcgtcaga ttttttaact
      841 ctcgatccat acccgctcca gaatgtttta aacatgcatc ttgcgaacaa ctttaggcaa
      901 acggtgttag tgatgaacaa aaaacaatca aaatcgacac cataaaaata aaaccactgt
      961 aatatcaatc cattacagta gtggtgatga cgaatgaaat ttcaaaaact agcctttttc
     1021 cgcgccgctc ccgccccgtg gcaggccacc ccaccgggag gacccgtcag cctgacagcc
     1081 atgacgaacg tctgatacag cgccctgcat gaatgacatc gggataatcc agaaaggaat
     1141 agcatcgtac ccacaagaat cagtgtaagt gtcctgtttc ttccaccccc gcacaggact
     1201 ggcgagcatg agggacaaac ccgcgaacca taaacgcggt aaaaacccgg tgtgcatcgt
     1261 ttttgattat tcccgcacac tcgcgcagaa ggagttcccc gtcgggctac ggtcatggtt
     1321 aatacgggaa tacggcgacg atacagcgca tgatgtgtca ggcttgaata cctttatccg
     1381 ttaaaaggga tatcagttaa gttatcccgt gtagggtata agccattatc aaagccactc
     1441 tgtaggaagt ggcttttgta atggcaataa aaagccccgc gaatgcgagg ctaaatcctg
     1501 gtatttgtaa tgactggctc ttatctcaac gcagcccctt accgcgcgca aaatgctcaa
     1561 tatcaagcat cagcaatgag atgtttaatc tggattcact ccagaagtga gcaccaccct
     1621 gtctacagag ccagatgtga aggatgatga gtaaaattat cgctatcatc gaaggcattg
     1681 cgtcctgatg tattcctgaa gcgttctcag tgctgtttgg tcgcggat
//
LOCUS       DAZRUF010000394         1719 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZRUF010000394 DAZRUF010000000
VERSION     DAZRUF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.397"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            10..345
                     /locus_tag="ACHXOL_004261"
     CDS             10..345
                     /locus_tag="ACHXOL_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR6811064.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(626..757)
                     /locus_tag="ACHXOL_004262"
     CDS             complement(626..757)
                     /locus_tag="ACHXOL_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR6811065.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1257..1333)
                     /locus_tag="ACHXOL_004263"
     tRNA            complement(1257..1333)
                     /locus_tag="ACHXOL_004263"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1297..1299),aa:Arg,seq:tct)
     gene            complement(1434..1509)
                     /locus_tag="ACHXOL_004264"
     tRNA            complement(1434..1509)
                     /locus_tag="ACHXOL_004264"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1474..1476),aa:Ile,seq:cat)
     gene            complement(1612..>1719)
                     /locus_tag="ACHXOL_004265"
     CDS             complement(1612..>1719)
                     /locus_tag="ACHXOL_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR6811066.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          536 a          437 c          317 g          429 t
ORIGIN      
        1 ggaaactaaa tgaagtggat tgtgattgat acagttatcc agccatcatg cggaatatct
       61 ttttcagtca tatggagtaa aataaaatta ataatctggt atcaatcgga tgctttctta
      121 cctcctgaaa gtatatttac actgactcac acaggcatca tgctcaataa caaagtgcta
      181 cctgtaacca tttacaacgt agtaccattc aataaaacat tctggaattc aatcaaaaat
      241 agccaggaat gccctacaaa tacagataac gtattgaatg aatgctttaa taaccgttgc
      301 actctgcaaa tatgtcctta tggactaaaa caacaaagtc cataaggagt ttactcacat
      361 ctgacaaaat caatataaac agcccctccg gagaggggct ggagagtggc gctatgtgcc
      421 attgcatggt gccgggtgcc tcccggtgaa ttcagtacca gcacctgaat ccgcgattat
      481 cccatatacc tactcgctga ttgcccctcc gcacaggggg attcaccatg ccagtttctt
      541 ttaacaaact ccccgcaaac cagacaacag tcaaccgcct gaattgtgaa gtatttaaaa
      601 atttctcccg ctaactgata cccggctaac agtctggcgt tttctttttc agcaacggga
      661 aagcaacaac caccacaccc gccaccagca caccgtcagc cagcactgac attatccggc
      721 tgctgcaatg ccattcacaa aaacagtaag caatcacttt ttaccgtaac aggtgataat
      781 ccagatatgt atctacccca gatgagtaat ccgaagttca tccataccac aggtcctggc
      841 tattctgttg tactcctgaa caagagcaaa taattctgaa ttagcaacca tgaactcatc
      901 gcaaaccctc tgtatagcat cactattcag aataataacg tctcttcccg aaagacgatc
      961 aggagtacag aacaaaactg tcaaacggct gaaggccttt gctcgtgctg cattgactat
     1021 atcaatacgc tgcctaagga tgaaacaccc cgacgcctca tcaatattca ctctacccac
     1081 accatatgaa tgataaatat ttaatgctga aaaaaccatt agaccgtata acaaacactc
     1141 aatcaatact taacagaact tttatttttg acaaacataa aatattttca acaatatcct
     1201 gagccaggta tatttcagta taaggctctg ccggaaggaa tctggaagaa tgaatatggc
     1261 gcgctgtact ggattcgaac cagtgaccga ttgcttagaa ggcaattgct ctgtccggct
     1321 gagctaacaa cgctgaatac cgataatgga ccgccatcgg ggacccgccc ccgcaccaac
     1381 aaccctgtta tcatgtcgtc tgctcttcct gataagctaa tggcggtttg tgatggtggc
     1441 ccttgctgga tttgaaccag cgacctggcg attatgagtc gctcgctctc accactgagc
     1501 taaagggccg ggagcagaat aataacggtc cgtaattaat tccgcaataa aaaacccgct
     1561 cggcggcggg ttgtagaaac tcttctaacg tcaggcataa aacgcccata gttatgacga
     1621 atttaccaca gattccggaa aaatcaacct agttaccttt tttaactgcc gctcagccca
     1681 tgcttcttca atatcaaacc gggtcaccag cgcatcata
//
LOCUS       DAZRUF010000395         1670 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZRUF010000395 DAZRUF010000000
VERSION     DAZRUF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.398"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..462)
                     /locus_tag="ACHXOL_004266"
     CDS             complement(<1..462)
                     /locus_tag="ACHXOL_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR6811067.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQ"
     gene            470..971
                     /gene="tnpA"
                     /locus_tag="ACHXOL_004267"
                     /pseudo
     CDS             470..971
                     /gene="tnpA"
                     /locus_tag="ACHXOL_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(989..1657)
                     /locus_tag="ACHXOL_004268"
                     /pseudo
     CDS             complement(989..1657)
                     /locus_tag="ACHXOL_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          426 a          397 c          381 g          466 t
ORIGIN      
        1 ctggctgaca gtgacatttt tcacaacacc cgtgacctga cggctattcc ggtagcgcat
       61 ccagcccagt ttcggcagaa aaatacggct gttttcctga tcgagcttaa cgccctgcgg
      121 gtagcggaat gcatcattct gtccccgctt tttgaatcgg ggaaaagcag cccgcttccg
      181 gaagaagttt ttgtaagccc gctcaaggtc tttcagtgac tgttgcaatg gctgtgaggg
      241 agaatcttta agccattgcg tttcagtggc atttttccac tcaaccagcc aggaagccat
      301 tttcccgtaa gggatgtatt tattaccggc ctcatgattc tcattctgac gtgccagagc
      361 acgattgaaa acgaaacgac atgcgcctgc gaagcgcctc atttcacact cctgttgacc
      421 acctggtctt aactggaatt taaatgcctg taatcgtttc acaggtcaaa tgatactttg
      481 gtctgtgaaa aaagaaaccg atatccggcg tggaaggcat tgtgttttcc tgatgcatgt
      541 tcacctggtc tttgtcacta gataccgacg ccagattttt tgacgcgaca gaaaaactac
      601 gcacttactt ttcaaatgta tgtgcttatt ttgaagctga actggttgaa atggatggcg
      661 aaccagatca cgtccatttg ttaattaact aacctcccaa actggcgata tccagtctgg
      721 taaacagcct caaaggcgta tcgggtaggt tactgcgacg agatcgacca gatattgcag
      781 tcaggtatta ctacaaaggc gttttgtgga gtcctggcta ttttgccagt agctgcggag
      841 gtgtgccaat atccgtcatc cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg
      901 aaaaccgcgc cttatatccc cgccctgaag gatggggttt tacggcgcac cggataaaaa
      961 gcgccatcta acgatggcgc gtctggaact actttttgtt aacgtcaaac atggtggcat
     1021 cggtcgccat gtcatcaata acttgtttaa tattttcgaa agccattggc gtactttcgt
     1081 tattcaggtc tttaccttcg ccctgacgaa cgactttgat aaccggttta ttagtcgctg
     1141 catcaatcag ctcaccttca aaatagaggc gagtatccat ggtacggtgg cctgtagcca
     1201 tttgcgtccc cgcaaccact aatgcaacag gaaccacttc atagaattgc agcccttctt
     1261 tgctggtatc tacaccggta atggccccac ggaaaatcag actacgcggc ccagcggtgg
     1321 taaccagtgg tttacgctgc gctatcgctt ctttcatttc ggtgttggta tagttcaaaa
     1381 ttttatccag aactttctgc cctacctagg tagaaggttt tggcaccgga taataagtga
     1441 ttgggttcca gacgatgctg tcatatttgc tttgatcaaa actcgggtct acccaacgta
     1501 aaacaggttt acctgttgct gaggttgttt cttttaaatc agagtaattg tttaaaaaac
     1561 cagaatattt atctggctgg gtgattttag aggcacaacc agacagagcc aacaagccgc
     1621 ataaagctgc aactttcgca aatgatgtgg tacgcatgaa tagtttcctg
//
LOCUS       DAZRUF010000396         1647 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZRUF010000396 DAZRUF010000000
VERSION     DAZRUF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.399"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..867)
                     /locus_tag="ACHXOL_004269"
     CDS             complement(<1..867)
                     /locus_tag="ACHXOL_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR6811068.1"
                     /translation="MTSPFIQQIADNRVCQVLSCLPEKFVVDFANGIDVAQEHNRTAG
                     GRTFFRRLKEGLTGKGAVRQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLAEQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     NGGSATVRLNTLDWLGGQSREQADNEWHEAVNWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSE"
     gene            complement(864..>1647)
                     /locus_tag="ACHXOL_004270"
     CDS             complement(864..>1647)
                     /locus_tag="ACHXOL_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR6811069.1"
                     /translation="FSVSAPGHHSWKRDFDPDSPEIKFSDRREALKLMTQIESTVTSL
                     HREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEGFTVKITFPAVSQ
                     LQTRIAVKTNLSALMEERTETVTRRRRQSGFWGKICGAFGTSDWGWENYKENVSRSVI
                     NINSVRKEVMSLTRAYFGELQASIEQNINQPVRQEIDDFFCTFREKVEQLRNTLIQSS
                     EDHKRDQQAQEQLTERLQALNERVPELITDSKALREELETLL"
BASE COUNT          358 a          468 c          390 g          431 t
ORIGIN      
        1 ttcagagagg cgttctgcag agcagagacg gggcatacga accggtaagt gttctgcagc
       61 ctgtgtggtt gaccagatca caggatgccg ctcttcactg caccagtccc ccagccagtt
      121 aaccgcttca tgccattcgt tatcagcctg ttcccggctc tgaccaccaa gccagtccag
      181 tgtgttcaga cgtactgttg cgctaccgcc attctcctgt gccatttgcg ttaatgcttt
      241 atgtctgagg atatccagta actggttaac ctggcctgtt tcaccctgac gtatcacatc
      301 accaaacgcg ccccagcgaa gctcttcaag cgccacataa caacgtccgg caggggaaaa
      361 tgacgcgtat cgcccggcgc tccaccatga gaatatctgc tcaagatgta actgtgcccg
      421 ctgaacctgg tcaacacggt ggagtttttc ttcaagatga ttcagtttct gatgaacctg
      481 ttctgccagg gtgagtaact gctcccgcgt gtctgccgaa taatgcgcca gcctggcagt
      541 atcactgacc agtgaactga ccctgtcgtt tacccgggta atcgcgtaat ttgtggtggc
      601 aagcgacgtt gtcagctccg tcagccagcg cagggacgcc tcaacgccct gtgcaagcga
      661 agcattgatg gcattctggc gtacagcgcc tttgccagtc agaccttctt ttaaacgccg
      721 gaaaaacgtg cgccccccgg ccgtgcggtt atgctcctgt gcaacatcaa taccgtttgc
      781 aaaatcaacc acaaattttt caggaaggca actgagtacc tgacataccc ggttatcagc
      841 aatttgttga ataaatggtg aggtcacagc agtgtctcca gttcttccct taacgcctta
      901 ctgtcagtaa tcagctcggg aaccctttca tttaatgcct gaaggcgctc cgtaagctgc
      961 tcctgtgcct gctgatcgcg tttatgatct tcagagctct gaatgagtgt gttacgtagc
     1021 tgttcgactt tttccctgaa tgtacagaaa aaatcatcga tctcctggcg gacgggctgg
     1081 ttaatattct gctcaatgga tgcctgcaac tccccgaaat atgcccgggt cagtgacatc
     1141 acttccttcc tgaccgagtt gatattgatc acactgcggc tcacattctc cttatagttt
     1201 tcccagcccc agtcactggt gccaaacgct ccacaaatct ttccccagaa accactctgc
     1261 cgacggcgac gggtgactgt ctcagttctt tcctccataa gcgcactcag atttgttttt
     1321 accgcgatcc tggtctgtaa ctggctgact gccgggaaag tgatttttac agtgaagccc
     1381 tcgtcctcca ggcgggcatt gatacgtccg gcaatatttt ctgtggcata cagggccgta
     1441 ccacgaaaac ctgtttcgat acccctgacg attttttcca gttcaggccg gaactgtgct
     1501 tcagcctcac ggtgcaggct ggtcacggtc gattcgattt gcgtcatcag tttaagggct
     1561 tccctgcgat cactgaattt tatctcgggg ctgtccggat caaagtctcg tttccatgaa
     1621 tgatggcccg gggcactgac actaaac
//
LOCUS       DAZRUF010000397         1646 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZRUF010000397 DAZRUF010000000
VERSION     DAZRUF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.400"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            46..1299
                     /locus_tag="ACHXOL_004271"
     CDS             46..1299
                     /locus_tag="ACHXOL_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6811070.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(1348..1467)
                     /locus_tag="ACHXOL_004272"
     CDS             complement(1348..1467)
                     /locus_tag="ACHXOL_004272"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR6811071.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
BASE COUNT          338 a          418 c          405 g          485 t
ORIGIN      
        1 ctatgtataa gcctgatcca caggcatatt tagcaaggat ttcaagtgag ccgtactaca
       61 actgttgatg gcgctccggc aagcgacact gacaagcaaa gcatttctca gccaaatcaa
      121 tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc tggcgctgtt ctctgccgga
      181 ctggcaacct ttgcactcct ctattgtgtg cagcctatcc ttccggtgct ttcgcaggag
      241 tttggcttaa cccccgcgaa cagtagtatt tcactgtcca tttccacggc gatgttggct
      301 attggtttgc tgtttactgg ccccctatcc gatgccattg gtcgcaaacc agtgatggtc
      361 acggcgctac tgttggcctc catttgtacg ttactttcga caatgatgac cagctggcac
      421 ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggcgtggc agctgttggc
      481 atgacttatc ttagcgagga aatccacccc agtttcgtgg ccttttcgat ggggttgtat
      541 atcagcggca actcaattgg cggcatgagc ggacgcttaa ttagtggtgt cttcacggac
      601 tttttcaact ggcgaattgc tctggcggca atcggttgtt tcgcgctagc ctcggcgttg
      661 atgttctgga aaatccttcc ggaatcacgc cattttcgcc cgacttcgct gcgccctaag
      721 acgttattta tcaactttcg tctgcactgg cgtgaccggg gattaccgtt attgttcgca
      781 gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacatcgg ctatcggttg
      841 atgctttccc cctggcatgt cagtcaggcc gtggttggct tattatcgct ggcttatttg
      901 accggtacat ggagctcacc caaagccgga accatgacca cccgctacgg gcgcggtccg
      961 gtgatgttgt tttcgacggg ggttatgctg tttggtttac tgatgacctt attcagctcg
     1021 ctgtggctga tctttgccgg aatgttactc ttctcagcag gattcttcgc agcccactca
     1081 gttgccagca gctggattgg cccccgcgca aaacgcgcta aaggccaggc ctcctcgctg
     1141 tatttgttca gttactatct ggggtcgagt attgccggga cgctgggtgg tgttttctgg
     1201 cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt cattgctctg
     1261 ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa aaataagccc ggactgcggt
     1321 aactacacgt ccggacttat tgcttgctca aaccaacgtt aatagccatc ctaaaataga
     1381 cgaagcgcca gccaattccc gcagcgcgtc tagcgtcatc aggattataa gtacccaaat
     1441 aaacggattc attttgctgt gtgtcattta ttactgatgc gcagttattc taccgctttg
     1501 taagtagtaa aatagttaac ccgatcaaga ctactattat tggtagctaa atttccctta
     1561 agtcacaata cgttattatc aatgctgtaa tttattcagc gtttgtacat atcgttacac
     1621 gctgaaacca accactcacg gaagtc
//
LOCUS       DAZRUF010000398         1630 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZRUF010000398 DAZRUF010000000
VERSION     DAZRUF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.401"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            71..325
                     /gene="bssS"
                     /locus_tag="ACHXOL_004273"
     CDS             71..325
                     /gene="bssS"
                     /locus_tag="ACHXOL_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR6811072.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            440..1558
                     /gene="solA"
                     /locus_tag="ACHXOL_004274"
     CDS             440..1558
                     /gene="solA"
                     /locus_tag="ACHXOL_004274"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR6811073.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPEQPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT          422 a          405 c          407 g          396 t
ORIGIN      
        1 aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta acacgaatta
       61 gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg tagggtggga
      121 catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc agaccccaaa
      181 taagtccgag caggaaggga ctgaagttgg tcaaacgctc tggttaacca ctgatgttgc
      241 cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg gtgatttcca
      301 ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag gcacctcacc
      361 ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct gcttaaacca
      421 tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg gttccgtagg
      481 cgctgccgcc gggtattatg caacccgcgc cggtttaaac gtgctaatga ccgacgccca
      541 tatgccaccg catcaacacg gcagccacca cggcgatacg cgattaattc gccatgctta
      601 tggtgaaggc gaaaagtatg tcccgctggt cctccgcgcg caaacgctgt gggatgaact
      661 ttcccgccac aacgaagatg atcccatttt tgtacgctct ggtgtcatta acctcggccc
      721 ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc aactcaacgt
      781 tgaaaagctc gatgcgcaag ggattatggc ccgctggcca gaaatacgcg tcccggacaa
      841 ctacatcggc ttatttgaga ctgattccgg ttttttgcgc agcgaactgg cgattaaaac
      901 ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc cggtcaccgc
      961 aattcgtcat gacgatgatg gcgtaactat tgaaacggtt gacggtgagt atcaggcgaa
     1021 aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc agcctgtcca
     1081 gcccgtacgt aaagtatttg cctggtatca ggccgatggc cgctatagcg tgaagaataa
     1141 attcccggcg tttaccggtg aactgcccaa tggcgatcaa tattatggtt ttccggcaga
     1201 aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt cagcggatga
     1261 acgtgttccg tttgcggaag tggtcagcga tggttcggaa gccttcccgt tcttgcgcaa
     1321 tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg ataattcgcc
     1381 tgacgaagat tttattatcg ataccctacc cggccacgat aatacactgc tcattaccgg
     1441 cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg ccgattttgc
     1501 gcaagacaaa aaaagcgatt ttgatttgac gccattcagg ctttcccgct tccaataatc
     1561 atcattacgg cctccagaat gaggccgttt ttacaggagt atttaatgcg tcggctgttg
     1621 cactatctca
//
LOCUS       DAZRUF010000399         1630 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZRUF010000399 DAZRUF010000000
VERSION     DAZRUF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.402"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..94
                     /locus_tag="ACHXOL_004275"
     CDS             <1..94
                     /locus_tag="ACHXOL_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR6811074.1"
                     /translation="RFYLEEQGWTLVNRGAIKNMTVEQLLKIHC"
     gene            105..293
                     /gene="dicB"
                     /locus_tag="ACHXOL_004276"
     CDS             105..293
                     /gene="dicB"
                     /locus_tag="ACHXOL_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR6811075.1"
                     /translation="METLLPNVNTTEGCFEIGVTISNTVFTEDAINKRKHERESLNKI
                     CIVSMLARLRLMQKGCWR"
     gene            290..481
                     /locus_tag="ACHXOL_004277"
     CDS             290..481
                     /locus_tag="ACHXOL_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR6811076.1"
                     /translation="MNTAFALVLTVILNTGEPAELVTGVYNSMKECVAAAAEQKIPGN
                     CYPVDKIIHQDNKEVPAGL"
     gene            575..>1630
                     /locus_tag="ACHXOL_004278"
     CDS             575..>1630
                     /locus_tag="ACHXOL_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFR6811077.1"
                     /translation="MSKVFICAAIPDEKATKEEGAKPVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESEPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMCPEIKLHAIGWIKHKCKPGAKWPEIQAEMRIWKKCREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPAIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATD"
BASE COUNT          498 a          362 c          391 g          379 t
ORIGIN      
        1 ccggttttat ctggaagaac agggatggac tcttgttaac cggggcgcaa ttaaaaatat
       61 gactgtagag caattactca aaattcattg ttgaggacgc gataatggaa acgttattac
      121 caaacgttaa tacgactgaa ggttgttttg aaattggtgt cactatcagt aacacggtat
      181 ttactgaaga tgccattaac aagagaaaac acgaacggga gtcattaaat aaaatatgca
      241 ttgtttcaat gctggctcgt ttgcgtctga tgcaaaaagg atgctggcga tgaatacagc
      301 atttgcactc gttctgaccg ttattcttaa tacgggcgaa ccagctgaac tggttaccgg
      361 agtatacaac tccatgaaag aatgcgtggc tgctgcagct gaacagaaaa ttcccggtaa
      421 ctgttatccg gttgataaaa tcattcacca ggataataaa gaagtcccgg caggtcttta
      481 aagcagccac gtaataaata tccggtttca tttttatatg ccagcaatgg cagggatttg
      541 ttcaccctta aatctgtaat gaggttaaaa caaaatgagt aaagtcttta tttgcgccgc
      601 cattccggac gaaaaggcaa caaaagaaga aggcgctaaa cctgtagcca ctgccattga
      661 agccggcgac gaacgccgcg cccgcgcaaa atttcactgg caattcctgg aacattatcc
      721 ggctgctcag gactgcgctt ataaatttat tgtctgcgag gataaacctg gtataccccg
      781 ccctgccctc gattcctggg atgctgaata tatgcaggaa aaccgctggg atgaggagtc
      841 tgcttccttt gtcccggttg agactgaatc cgaaccgatg aacgtcactt ttgacaagct
      901 ggcccctgaa gtacagaacg ctgtcatggt taagttcgac acatgtgaaa acatcaccgt
      961 tgatatggtg attagcgcgc aggaactgtt gcaggaagac atggcaacat tcgacggaca
     1021 tatcgttgaa gcgttgatga aaatgccaga agttaacgcc atgtgtccgg agattaaact
     1081 gcatgccatc gggtggatta agcataaatg taagcctggt gcaaaatggc cagaaattca
     1141 ggcagagatg cgcatctgga aaaaatgtcg cgaaggtgaa cgcaaggaaa ccggaaaata
     1201 cacgtctgtt gttgatctcg cccgtgccag agccaatcaa cagcacactg aaaattcaac
     1261 aggaaaaatc agcccggcca ttgctgccat tcatcgcgaa tacaagcaga catggaaaac
     1321 actggatgac gaactggcct acgctctctg gcctggtgat gtggatgccg gaaacattga
     1381 cggcagcatc catcgctggg caaaaaatga agttatcgac aacgaccgcg aagactggaa
     1441 gcgtatctcg gcatcaatgc gcaaacagcc tgatgccctt cgctacgacc gccagactat
     1501 ttttggcctt gtccgtgaac gtccgatcga cattcacaaa gaccctgtgg cactgaacaa
     1561 atacattact gaatacctga ctacaaaggg cgtgtttgaa gatgaaggaa caaatcagag
     1621 cgcaactgat
//
LOCUS       DAZRUF010000400         1586 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZRUF010000400 DAZRUF010000000
VERSION     DAZRUF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.403"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..1500
                     /locus_tag="ACHXOL_004279"
     CDS             <1..1500
                     /locus_tag="ACHXOL_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFR6811078.1"
                     /translation="NDKNAANNSKQTQMVNGTFIYWLNLNTPGNLRGITTRCVLLDEV
                     SSCEITDEGNPIKLAEARTSTFGSDSLVVVSSTPLYKDDLINAEYNLSDKRRYFVTHT
                     CGHEYTFEWEQVAFEFKQLENGRSIPDSTTTRLLCPHCNEGIDEHTRHQMVDNGRWIA
                     TNPDGEPGVVGYQISRMYSPLNTITEMVSKFADALYNFNLQTFYNNELGLPFEDEYQK
                     ELDILQLESLREDEFNLYKIPESTLALCISVDQQGDRLESLLLGFDEKNIYVLGHEFF
                     YSHDCTKIEAPAWKDLDQFCRQDFSTVSGRIVPTLAVFIDAGNGNATDTVKKFTTRWS
                     KYHPIKGSSSTTGELFKTSTQAGYKLQILNVHEQKNTIRRLLNLMLSSEADNAPVKLR
                     FSSTLPSDFFEQLSAEELKPAGGKLVWRLKKGQRRNEALDLICYGMIAIAYAQSKLGT
                     QPFRKLRDYKAQETTKYEINKVEEPKPETVQKPKRNRRTGMGSNWFGKR"
BASE COUNT          518 a          311 c          341 g          416 t
ORIGIN      
        1 aatgataaga acgccgccaa taactctaaa caaacacaaa tggtgaacgg tacttttatc
       61 tattggttga acctcaacac gccggggaac cttcgcggta tcacaacacg ctgtgttctg
      121 ttagatgagg tcagtagctg cgaaatcact gatgaaggga acccaatcaa actggcagaa
      181 gcacgtacca gtaccttcgg tagtgactca ctggtggttg tatcttccac gccattgtat
      241 aaagacgatt taatcaatgc tgagtacaac ctcagtgata aacgtaggta ctttgttact
      301 cacacatgcg gtcatgaata tacttttgaa tgggaacaag tagcgtttga atttaagcaa
      361 ttagaaaatg gtagatccat tcctgatagt actactactc gtttgttatg ccctcattgc
      421 aatgagggaa tagatgaaca tacacggcat cagatggttg ataatggtcg atggattgca
      481 actaatccag atggggaacc gggtgtagta gggtatcaaa tcagtagaat gtattcacct
      541 ctgaatacta ttactgaaat ggtttcaaag tttgctgatg ctctatataa cttcaatctt
      601 caaacctttt acaataacga attgggcttg ccattcgaag atgagtatca aaaagaatta
      661 gatattctcc aattagaatc cctacgagaa gatgaattta acctttataa aattcctgaa
      721 agtaccttag cgttgtgtat ttcagttgat cagcagggtg ataggctgga aagtctgctt
      781 ttaggttttg atgaaaagaa catttatgta cttgggcatg agtttttcta ctcccacgat
      841 tgtacgaaga ttgaagcccc agcttggaag gatttagatc agttttgccg tcaggatttc
      901 agtactgtat caggtcgcat agtacctaca ctggctgtgt tcattgatgc agggaacggt
      961 aacgctacgg atactgtgaa aaaattcact acacgttgga gtaagtatca tccgatcaag
     1021 ggtagcagct ccactactgg tgaactattc aagaccagta ctcaggcggg gtacaaacta
     1081 cagatcctaa acgtacatga acagaagaac acgatacgcc gcctcctgaa cctgatgcta
     1141 tccagtgagg cagataacgc cccggttaag ctccgcttca gtagtacctt accatcagat
     1201 ttctttgagc aactttcagc agaggagtta aaacccgctg gtggtaagtt ggtatggcga
     1261 ttaaagaaag gtcaaagaag aaatgaggct ttagacctaa tctgttatgg aatgatcgcc
     1321 atagcatatg cccaatcaaa attaggtact caaccattca ggaagttacg cgattataaa
     1381 gcacaggaaa ccactaaata cgagataaat aaagtagaag aaccaaaacc agaaacagta
     1441 caaaaaccaa aacggaatag acgtactgga atgggatcaa actggttcgg taaaagataa
     1501 ggatatcaaa aatgacactt ctaccagaaa agatctacat ggtatcgaat cccatggatc
     1561 taacggtatt agtacccgct gctact
//
LOCUS       DAZRUF010000401         1558 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZRUF010000401 DAZRUF010000000
VERSION     DAZRUF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.404"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>1558
                     /locus_tag="ACHXOL_004280"
     CDS             <1..>1558
                     /locus_tag="ACHXOL_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HFR6811079.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGT"
BASE COUNT          538 a          306 c          293 g          421 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtac
//
LOCUS       DAZRUF010000402         1554 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZRUF010000402 DAZRUF010000000
VERSION     DAZRUF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.405"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..1548
                     /gene="espL1"
                     /locus_tag="ACHXOL_004281"
                     /pseudo
     CDS             1..1548
                     /gene="espL1"
                     /locus_tag="ACHXOL_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
BASE COUNT          491 a          326 c          289 g          448 t
ORIGIN      
        1 agcatactgg attatattga acaggaacag ttaactgccg aagatcgtaa ccattgtatg
       61 aaactgtcta aaaaaatcca tagagaaaaa actatacaac caacagtaaa tctcaatggt
      121 agtgcatttt tttcgcaatc tccttctgac gctatttttt gtcgccatct gtcattgcaa
      181 tacgcccttg attcattgag aaatggaaaa ggcaaagtca acctgattaa acactactcc
      241 tccgttgaat ccatacagca gcatgtcccc ttagtccggg acgcggagtt cagagcatta
      301 cttcgccatc ctcctgcagg gagtcgcgtt atcgcgagta aggattttgg cttcgcttta
      361 gatattttct tttgtcgaat gatggcaaac aatgtcagtc atatgtccgc gattttatat
      421 atagacaatc atactttgtc agtaaggcta cgaataaagc agtcagtgta tggacaatta
      481 aattatgttg tgtctgttta cgacccgaac gataccaacg ttgccgtcag aggcacccac
      541 agggcagcac ggggctttct ctcgcttgat aagttcatca gttcagctcc cgatgctcag
      601 acctgggctg ataggtatgt tcgcaactgt gcaattgcta ttcttcctct attacctgag
      661 ggagttccag ttgctatttt agcgggtatc acgacacgaa tgccatttgc ccctatacat
      721 ccatcggcaa tgttgttaat aatggccaca ggccagactc aatagcttat tacactattc
      781 aaacagttgc ccatactccc tgaaaaagaa atcattgaaa taataactgc gcagaatagc
      841 gttggtacac ctgctttatt tctggctatg atgaacggac aaactgacaa cgtaaaaata
      901 tttatgcaag aaattcagtc actggtatac aatcatatca ttcatgaaga taatctggtt
      961 aaattactgc aaactaaaag tgctaacgaa acacctggac tctatatctc catgttgtat
     1021 ggattcgatg aaataatcga tatctttctg aatgcattaa ccactccaat agcacaagaa
     1081 cttttaaaca aaaaaatggt gatgagtatt ttagccatga aaatacatga tggtgagcca
     1141 ggattatacg ccgcaatgga aaataatcac cctttgtgtg tcacacggtt cctctctaaa
     1201 attaatggca tcgcctttaa atacaagttg agcaaagcta acatcatgga tttattaaaa
     1261 ggagctacag cacagggaac ccctgcatta tacatcgcca tgagcaaggg taatgaagac
     1321 gtcgtgttat cttatatatc gacgctgggt gcttttgcaa aaaaacattc ttttagtcaa
     1381 catcagttat ttacactatt ggccgctaaa aatcatgaca acatgtcagc tgttcatata
     1441 gccattcatc ataagcatta taaaactgta gaaacatatt atgctgctat aaatgtcatc
     1501 agccaaagcc tgagttttag tgctgatgaa ataaagacgt atttataacc agct
//
LOCUS       DAZRUF010000403         1554 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZRUF010000403 DAZRUF010000000
VERSION     DAZRUF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.406"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            15..779
                     /gene="nfeF"
                     /locus_tag="ACHXOL_004282"
                     /gene_synonym="yqjH"
     CDS             15..779
                     /gene="nfeF"
                     /locus_tag="ACHXOL_004282"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR6811080.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(833..908)
                     /locus_tag="ACHXOL_004283"
     tRNA            complement(833..908)
                     /locus_tag="ACHXOL_004283"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(873..875),aa:Ile,seq:cat)
     gene            1033..1539
                     /gene="mug"
                     /locus_tag="ACHXOL_004284"
     CDS             1033..1539
                     /gene="mug"
                     /locus_tag="ACHXOL_004284"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR6811081.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT          363 a          388 c          441 g          362 t
ORIGIN      
        1 cgaaggcgat aacaatgaat aactcccccc gctacccgca gcgcgttcgc aatgatctgc
       61 gcttccgtga actgactgtg ttacgcgttg agcgcatcag cgccggtttt cagcgcattg
      121 tcctcggcgg cgaggcgctg gacggtttta catcgcgcgg ctttgacgat cacagcaaac
      181 tcttctttcc tcaacctgac gctcactttg tgccgccaac ggtaacggaa gagggcatcg
      241 tctggccgga aggaccacgc ccaccgtcgc gtgactatac gccgctgtat gacgaactac
      301 gccatgaact ggcgatcgat ttctttattc acgacggtgg ggtcgccagc ggctgggcga
      361 tgcaggcgca accgggcgat aaacttacgg ttgcaggtcc gcgcggttcg ctggtggtgc
      421 cggaagatta cgcgtatcag ctgtatgtct gcgatgaatc cggaatgccc gcactgcgcc
      481 gccgcttgga aacgttgagc aaacttgccg ttaaaccgca agttagtgcg ctggttagcg
      541 tgcgggataa cgcctgtcag gattatctcg cgcaccttga tggttttaat atcgaatggc
      601 tggcacatga tgagcaggcg gtagatgcgc gtctggcgca gatgcaaatc cctgccgatg
      661 attacttcat ctggataacc ggcgaaggta aagtcgttaa gaatttaagc cgccgctttg
      721 aagcggaaca gtatgaccca cagcgggtac gtgcagcggc ttactggcac gcaaagtaac
      781 tgatatataa aaagaaaaag gctgacgatt tctcgtcagc ctttgctata tctggtggcc
      841 cctgctggac ttgaaccagc gaccaagcga ttatgagtcg cctgctctaa ccactgagct
      901 aaggggccat ggtagcggat tataaagtaa ctccgtgtcg caatccagcc attaccgtgc
      961 gcctgctgtt tttataaaca atgtattttc aatccgttat acttttcctg gtgatgttag
     1021 aaaggagtaa atatggttga ggatattttg gctccagggt tacgggtcgt gttttgcggt
     1081 atcaaccctg ggctttcatc cgccgggact ggttttccct ttgctcatcc agcaaatcgc
     1141 ttctggaagg tgatatatca ggccgggttt accgaccgtc agttgaagcc gcaggaggca
     1201 cagcatctgc tggattatcg ttgtggcgtc accaaactgg tagaccgtcc aacggtgcaa
     1261 gccaatgaag tttcaaagca ggagctgcac gcaggtgggc gtaagctgat tgaaaaaatt
     1321 gaagattatc agccgcaggc gttggcgatt ctgggcaaac aagcatatga acagggattc
     1381 agccagcgcg gtgcacagtg ggggaaacaa acgctcacca ttggttcgac gcagatttgg
     1441 gtgctgccaa atcccagcgg tttaagtcgc gtttcactag agaaactggt tgaagcgtat
     1501 cgcgagctgg accaggcgct ggtagtgcgt gggcgataaa aaacgccacc gaaa
//
LOCUS       DAZRUF010000404         1549 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZRUF010000404 DAZRUF010000000
VERSION     DAZRUF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.407"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            351..476
                     /locus_tag="ACHXOL_004285"
     CDS             351..476
                     /locus_tag="ACHXOL_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR6811082.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="ACHXOL_004286"
     CDS             complement(921..1397)
                     /locus_tag="ACHXOL_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR6811083.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          358 a          406 c          384 g          401 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atccaggatg
     1501 cgacaacgtt gtttcatcct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAZRUF010000405         1535 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZRUF010000405 DAZRUF010000000
VERSION     DAZRUF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.408"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>1535
                     /locus_tag="ACHXOL_004287"
     CDS             <1..>1535
                     /locus_tag="ACHXOL_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR6811084.1"
                     /translation="EAKNDKVRKRLGIKGGFYWAEAKKLSIAISRGAVAIDDAGYDED
                     DFKKPVRVNLPVVGDLPPEGVFDTEFCNRYEKGGEDGITMVFIASSPSVQDKPASTDN
                     TNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEELP
                     AVTALAVSHKTSLLDPLEIRDLHKLVRDTDKVFPNPGNSSLGLITAFFEAYLNADYTD
                     RGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARSM
                     DLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIGI
                     EVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQPS
                     CAMADEQATAETVEPDATEHHQDTQPLDAQSQVNAVDAKYQKLRTELHEARKNIPPKN
                     PVDADKLLAASRGEFVEGISDPNDSKWIPGHHISSNEVKKTENKVRQTEDKQHQNSEP
                     EER"
BASE COUNT          440 a          400 c          372 g          323 t
ORIGIN      
        1 acgaagcaaa aaatgacaaa gtaagaaaac gcctcggtat taaaggcggt ttttactggg
       61 ctgaagcgaa aaaattatcc attgccatct cccgtggtgc tgttgcgatt gacgatgctg
      121 gctacgatga agatgacttt aaaaaacctg ttcgcgtcaa tttgcccgtt gttggtgacc
      181 tcccaccaga aggcgtgttt gatacggaat tctgcaaccg ttacgaaaaa ggcggggaag
      241 atggcatcac aatggtattt atcgcatctt ctccctctgt tcaggacaaa ccagccagca
      301 ctgacaatac caacgtcaac ggcgaagaca tgacagaaat tgaggagaac atgctcctgc
      361 cagtttccgg tcaggaactg cccattcgct ggcttgctca acacggcagc gaaaaaccgg
      421 taacgcacgt ttcacgcgac ggactccagg cattacacat tgctcgggct gaagaactac
      481 cggctgttac tgccctggct gtttcccaca aaaccagcct gctcgacccg ctggaaattc
      541 gcgatctcca caaactggtt cgtgacactg acaaagtttt ccctaatcct ggtaattcaa
      601 gcctgggact gataactgct tttttcgaag catacctgaa cgctgactac accgatcgag
      661 gactgctgac aaaagagtgg atgaagggta atcgtgtttc acacatcact cgcacggctt
      721 ccggtgctaa tgctggcggc ggaaacctca ccgatcgcgg cgaaggtttc gtacacgatc
      781 tgacgtcact ggcgcgcgac gtagccactg gcgtactggc ccgttcaatg gatctggaca
      841 tctataacct tcatccggca cacgctaaac gcattgagga aattatcgct gaaaataaac
      901 cgcccttttc tgttttccgc gacaaattca tcaccatgcc tggcgggctg gattattccc
      961 gcgccatcgt ggttgcgtcc gtaaaagaag caccaattgg gatcgaggtc atccccgcgc
     1021 acgtcactga atatctgaac aaggtactga ctgaaaccga tcatgccaac cctgatccgg
     1081 aaatcgtgga tattgcctgc ggtcgctcct ctgccccgat gccgcagcgt gtaacagaag
     1141 aaggaaaaca ggatgatgaa gaaaaaccgc aaccatcttg cgcaatggca gatgaacagg
     1201 caacggctga aacagtggaa ccggatgcaa ctgaacatca tcaggacacg cagccgctgg
     1261 atgctcagtc acaggtaaat gctgttgatg cgaaatatca gaaactgcgg acagaactcc
     1321 atgaagcccg gaaaaacatt ccgcccaaaa atcctgtcga tgcagacaaa ttactggctg
     1381 cctctcgcgg agaatttgtt gaagggatta gcgacccgaa tgattcgaaa tggattcccg
     1441 gtcatcatat ctcctcaaat gaagtcaaaa aaacggaaaa taaagtgcga cagactgaag
     1501 ataaacagca ccagaacagc gaaccggagg agcga
//
LOCUS       DAZRUF010000406         1530 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZRUF010000406 DAZRUF010000000
VERSION     DAZRUF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.409"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..95
                     /locus_tag="ACHXOL_004288"
     CDS             <1..95
                     /locus_tag="ACHXOL_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811085.1"
                     /translation="EVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            301..>1530
                     /gene="gltA"
                     /locus_tag="ACHXOL_004289"
     CDS             301..>1530
                     /gene="gltA"
                     /locus_tag="ACHXOL_004289"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR6811086.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPR"
BASE COUNT          379 a          385 c          369 g          397 t
ORIGIN      
        1 atgaagtcgg tatttcacct aagattaact tatgtaacag tgtggaagta ttgaccaatt
       61 cattcgggac agttattagt ggtagacaag tttaataatt cggattgcta agtacttgat
      121 tcgccattta ttcgtcatca atggatcctt tacctgcaag cgcccagagc tctgtaccca
      181 ggttttcccc tctttcacag agcggcgagc caaataacaa acgggtaaag ccaggttgat
      241 gtgcgaaggc aaatttaagt tccggcagtc ttacgcaata aggcgctaag gagaccttaa
      301 atggctgata caaaagcaaa actcaccctc aacggggaca cagctgttga actggatgtg
      361 ctgaaaggca cgctgggtca agatgttatt gatatccgta ctctcggttc aaaaggtgtg
      421 ttcacctttg acccaggctt cacttcaacc gcatcctgcg aatctaaaat tacttttatt
      481 gatggtgatg aaggtatttt gctgcaccgc ggtttcccga tcgatcagct ggcgaccgat
      541 tctaactacc tggaagtttg ttacatcctg ctgaatggtg aaaaaccgac tcaggaacag
      601 tatgacgaat ttaaaactac ggtgacccgt cataccatga tccacgagca gattacccgt
      661 ctgttccatg ctttccgtcg cgactcgcat ccaatggcag tcatgtgtgg tattaccggc
      721 gcgctggcgg cgttctatca cgactcgctg gatgttaaca atcctcgtca ccgtgaaatt
      781 gccgcgttcc gcctgctgtc gaaaatgccg accatggccg cgatgtgtta caagtattcc
      841 attggtcagc catttgttta tccgcgcaac gatctctcct atgccggtaa cttcctgaat
      901 atgatgttct ccacgccgtg cgaaccgtat gaagttaatc cgattctgga acgtgctatg
      961 gaccgtattc tgatcctgca cgctgaccat gaacagaacg cctctacctc caccgtgcgt
     1021 accgctggct cttcgggtgc gaacccgttt gcctgtatcg cagcaggtat tgcttcactg
     1081 tggggacctg cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga agaaattagc
     1141 tccgttaaac acattccgga atttgttcgt cgtgcgaaag ataaaaatga ttctttccgc
     1201 ctgatgggct tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac cgtaatgcgt
     1261 gaaacctgcc atgaagttct gaaagagctg ggcaccaaag atgacctgct ggaagtggct
     1321 atggagctgg aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa actgtacccg
     1381 aacgtcgatt tctactctgg tatcatcctg aaagcgatgg gtattccgtc ttccatgttc
     1441 accgtcattt tcgcaatggc acgtaccgtt ggctggatcg cccactggag cgaaatgcac
     1501 agtgacggta tgaagattgc ccgtccgcgt
//
LOCUS       DAZRUF010000407         1527 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZRUF010000407 DAZRUF010000000
VERSION     DAZRUF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.410"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            99..362
                     /locus_tag="ACHXOL_004290"
                     /pseudo
     CDS             99..362
                     /locus_tag="ACHXOL_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(399..956)
                     /locus_tag="ACHXOL_004291"
     CDS             complement(399..956)
                     /locus_tag="ACHXOL_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR6811087.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(953..1228)
                     /locus_tag="ACHXOL_004292"
     CDS             complement(953..1228)
                     /locus_tag="ACHXOL_004292"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR6811088.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1414..1527
                     /locus_tag="ACHXOL_004293"
                     /pseudo
     CDS             1414..1527
                     /locus_tag="ACHXOL_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          424 a          326 c          368 g          409 t
ORIGIN      
        1 gccgcagaca cgtcgtatgc aggaacgtgc tgcggttagt ttgtgagctt tcgatagtgg
       61 ttgttatttt tgcccttatt tgttccggag gccatggttc aatggtccgt ctgccccctg
      121 tggtgatgtc agcaaaatca gccactgcgc gaaccacaat agcccgggaa gatgctgaag
      181 atcaccaggt aaagctgtca gcgcagaaac tggaagaact gctcgcatca atggttaagg
      241 atgaggttga tcgcaatgat gggatttatt gacgtcagcg agagcagaag gaagaactga
      301 ataacctgaa tgatttacgc tcaatcagag cgatggcgat tagcggcaat cacgctcgat
      361 aaaattaacc tcggtgatca tccggggttt tgcattcatt aaaaccgcat caacctttcc
      421 accaattgtt ctttacgggc aacgatccac ccatgttgct ccagataaaa tttaaaccgt
      481 tccagagtgc ataccatcgc atcggcgggg actttttctg tgaactcgac ctgaccgtgt
      541 ttatcgaagt ggatcagtaa tgcgcatcca ttatttgggg cgggggagtt ttgtgctgca
      601 ggaggacgtt tgtggagttc ttcttccatt gcgttaaatt tttcaatgta agcttctttt
      661 agtctggctg cttttttgcc agtaaatccc attacgagga acatgaagcc gtcttttgtg
      721 atcaggtagc atcgtgattc acgttctgcg ccgttgccaa tttcgacagt ctgaacatcg
      781 gccgaaaagt tggctgatgt aaattcggga gagcagtcga gtgtgtcaat cttagctaaa
      841 acgtgtttat gttgtttatc gaagtagtcg gcaacagctt gagaggtggt aaccaggcga
      901 ccatctttga aaaagacttt tggtgactcg ttagaaaaag ttatagctgt attcatatgt
      961 gatgactcca attaattgga tgatcaccac ctctgacgcc aatcagggag gtggtgagac
     1021 gtacagggtt ggcgtaaccg gtcatcacaa aacccggcgc ggatttctcc gcccctgcac
     1081 gccccaccat aatgcgaatg tggcaatgct taacgcaaaa aaaaccgcaa acgcggctat
     1141 gcgttgtgat gttatccgag acgccaatcc cgacaaccga ttttgcggtt gtgtgaggaa
     1201 tatagccccg gagaacaaaa actgtcaacg ggcaaaaaaa tttccgttat caggaatgta
     1261 taaaattaaa gcgatcgagc aaaaaatcct ttctgcaaaa ctaaaaaaga tttcaataaa
     1321 atcaggcagt taaaaacacg gaaaaacggt ataaaaaagc gcctcaaaat cctgtttttt
     1381 ctttcagata caatgtctta ttgcattttt taaaacatgt cagtgtggta catggatatc
     1441 gataccatcg ccaattataa actgggcggt gcacagcaac atgatagcgt acgcctcgat
     1501 ccgtgggtgt ttatgttctc agcagga
//
LOCUS       DAZRUF010000408         1498 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZRUF010000408 DAZRUF010000000
VERSION     DAZRUF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.411"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            39..482
                     /gene="cmtB"
                     /locus_tag="ACHXOL_004294"
     CDS             39..482
                     /gene="cmtB"
                     /locus_tag="ACHXOL_004294"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR6811089.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            510..>1498
                     /gene="cmtA"
                     /locus_tag="ACHXOL_004295"
     CDS             510..>1498
                     /gene="cmtA"
                     /locus_tag="ACHXOL_004295"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR6811090.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
BASE COUNT          347 a          333 c          385 g          433 t
ORIGIN      
        1 cgccggacgg taccctaaaa ataaaataag gataatttat gcggcttagt gactattttc
       61 cagaatcatc aatctcagtt atacattcag caaaagattg gcaggaagct atcgatttct
      121 cgatggtatc attgctggat aaaaactata tcagcgagaa ttacattcag gcaattaaag
      181 attccaccat taacaatggc ccttattata ttctcgcacc aggcgtggca atgcctcatg
      241 cgcgaccgga atgtggggcg cttaaaaccg ggatgtcttt gacattactt gaacaaggtg
      301 tttattttcc ggggaatgac gaaccaataa aattactcat cggcctttct gctgccgatg
      361 ccgattcgca cattggcgct attcaggcgt taagtgaatt actgtgcgaa gaagaaatac
      421 tcgaacaact cttaacagca gcatcagaaa aacaattagc ggacataatc agccgcggat
      481 aattactctc tcctctacag gataatatta tggaaaacaa gtctgctcgt gcaaaggtcc
      541 aggcttttgg gggctttttg acggcaatgg tcatccccaa tattggtgct tttattgcct
      601 ggggttttat tactgcgtta tttattccca ccggttggct gcctaacgaa catttcgcca
      661 aaattgtcgg cccgatgatt acctatttat tgcccgtgat gattggttct accggtggtc
      721 atctggtcgg cggtaaacgc ggcgcggtca tgggcggaat aggtactatt ggtgtgatcg
      781 tcggcgcaga gatcccgatg ttccttggct caatgattat ggggccgctc ggtgggctgg
      841 tcataaaata tgttgataag gcactggaaa aacgcatacc tgccggtttt gagatggtta
      901 tcaataactt ctcattaggt atcgcgggga tgctcctttg tctgctgggt tttgaagtta
      961 tcggcccggc ggtgttaatt gccaatactc tcgtcaaaga gtgtattgag gcgctggtac
     1021 atgcgggtta tctgcctctg ctgtcagtca tcaatgaacc ggcgaaagtg cttttcctca
     1081 ataatgcgat cgatcagggc gtctattatc cgctgggaat gcaacaggct tcggttaacg
     1141 gtaaatccat cttctttatg gtggcgtcca atcctgggcc tggcttaggg ctgctgctgg
     1201 cgtttacctt gtttggtaaa gggatgagta aacgttctgc gcccggggcg atgattattc
     1261 acttcctcgg tgggatccac gaactgtatt tcccgtatgt gctgatgaag ccgctgacca
     1321 ttattgccat gattgcgggc ggtatgtctg gcacctgggt gtttaactta ctggacggtg
     1381 gtctggtggc tggcccaagt ccggggtcta tctttgctta cctggcactg acgccgaaag
     1441 gctcgttcct ggcgacaatt gccggtgtta cggtagctac cctggtgtcc tttgctat
//
LOCUS       DAZRUF010000409         1494 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZRUF010000409 DAZRUF010000000
VERSION     DAZRUF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.412"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            233..>1494
                     /gene="ipaHb"
                     /locus_tag="ACHXOL_004296"
     CDS             233..>1494
                     /gene="ipaHb"
                     /locus_tag="ACHXOL_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaHb"
                     /protein_id="HFR6811091.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFTV
                     AADATESCEDRVALTWNNLRKTLLVHQAS"
BASE COUNT          454 a          357 c          256 g          427 t
ORIGIN      
        1 atgttgtagt atcttactca agactaatgt cttctggtat aaatcctctg cattgtgaat
       61 atgtaaaaca tcaaaaaaag atgaaccaaa tatacgaaaa aagcaaatat acttaccagg
      121 gtagaaagca tgatataaca ctcattccct tatggcgaat attctgaatc accctcctca
      181 ttaggagaga tacattaaat acaatttatt cacaatctaa ttccatatcc atatgcgaga
      241 aattaatatg ctcagaaata tttcatcctg tttatttcca catatcagca caattacatc
      301 ccccaaccat tatttgtccg aatgggatga ttgggagaaa caggggttac cggaagaaca
      361 gcgtactgag gcggtaagaa gacttcgtgc atgtcttacc tctaaggggc ataaactgga
      421 cctgcgagcc ttggcgcttt cctcgttacc tgtactccct gcttgcatta aaaagcttga
      481 tgtgagctgt aataaattaa ccatccttac tgatttacct gaaaatatta aagaacttat
      541 tgcaagagat aatttcttaa cacatatatc tgcattacca cattatctaa taactttgga
      601 tgtgtccgaa aatcaattag agaatctgcc gttattacca gacaccatca aatcactaag
      661 cgcagagtat aataggttat ccacactgcc ttcattaccc ttgaatttaa aaaaacttga
      721 ggttaggaac aacgaactgc aaactcttcc atctctgcct tctaatctta agatacttaa
      781 ggttgcgcac aaccatctta ctgaactgcc ccctttacct aggagactgc aacttctttt
      841 tgcatatagc aatagattaa gcaacttacc aaacatccaa gaaaatatta tcatgagaag
      901 atttttttat tttgaaaaca accaaataac tacaatcccg acaaatcttt ttcgtttaga
      961 tcctcatata actattgaga ttgcaaataa ccccttatca gatcaaactc tgctattctt
     1021 aatacagcaa acttcggttc caaattttaa cgggcctcag tttcgtattt ccctgtcaga
     1081 ccaaaacaga ctgtttttac gccagatgtt gccgcaaaat ttacattcgc gccatatcag
     1141 agtcatcact gaaggggggc agaactttca gatcccccct cttcccgaaa ctgtggcagc
     1201 ctggtttcct gaagcagatc gtcgggaggt ttctacacaa tggacttctt tttccaccga
     1261 ggagaattcc cgggcattct ccgcgttcct tgaccgcctt tccgataccg tctctgcacg
     1321 caatacctcc ggattccgtg aacaggtcgc tgcatggctg gaaaaactca gtgcctctgc
     1381 ggagcttcga cagcagtctt tcactgttgc tgctgatgcc actgagagct gtgaggaccg
     1441 tgtcgcgctc acatggaaca atctccggaa aaccctcctg gtccatcagg catc
//
LOCUS       DAZRUF010000410         1481 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZRUF010000410 DAZRUF010000000
VERSION     DAZRUF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.413"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..1011)
                     /gene="cadA"
                     /locus_tag="ACHXOL_004297"
     CDS             complement(<1..1011)
                     /gene="cadA"
                     /locus_tag="ACHXOL_004297"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR6811092.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1091..1459)
                     /gene="cadB"
                     /locus_tag="ACHXOL_004298"
                     /pseudo
     CDS             complement(1091..1459)
                     /gene="cadB"
                     /locus_tag="ACHXOL_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          413 a          324 c          371 g          373 t
ORIGIN      
        1 ggtgtaaggc acccacgcgg agtcaaagtg gatggatttc acatccagtg ttttcttgat
       61 gaagtcggtg ttgtacagca gaccatcata ggtagagttg gtaattacag catgtaccgg
      121 ccaggttgcg tttggtgttt ctttcacgcg cttagcaatg gtagcgtgct ggaattcact
      181 ctgtgggata ccaccaagaa taccgtaagc gttacgggtc gggcggaaat agattggcgt
      241 aacatcgctc atcatcatca ggtgggtcag cgatttgtgg cagttacggt caatcagaat
      301 ggtgctgcct gccggagcag agtacatgcc aacaattttg ttcgcagtgg aagtaccgtt
      361 ggtcaccatg tagctgcggt ctgcgttaaa gacgcgagcg atatactgtt ctgcttcttt
      421 gtgtggacca ctatgatcca gcagagaacc cagttcagat actgaaatgg aaatatcaga
      481 tttcatggta ttcggaccaa agaaatcata gaacaggcta cctaccgggc ttttctggaa
      541 tgcagtaccg cccatgtgac caggagtaca gaaagtatat ttaccttcac gaacatattt
      601 aaacagtgct ttagtcagcg gaggcagaat agtgttgata tattcgtcag tggtctgctt
      661 gatcttatta gcaatatctt cagcagcacc cagcgcatat tcaaagaagc taatctgtaa
      721 acgcaggtca ttcaggctta catcgagagt ggaatacgta ttagcgaacg cgtacaacgg
      781 caggttctcg ttcattttgc taatttcttc gcacagctcg agattatatt tatcccagtc
      841 aaaaataacg ccgcacagac gcgcattgtt ttcgatcagt tttaataagt cgtcacggtc
      901 gttcgggtaa acaatctgga agttcagacg ttcaagcgcg cgatgaagtt cacggatggg
      961 ttcttcttta aaataaaccc ccatgtgatt caatattgca ataacgttca tagtcatatc
     1021 tccaggtaaa aaaggcccct cccaacacat gggacaaaat gaaaggagga gcctcggaaa
     1081 atacttttaa ttaatgtgcg ttagacgcgg tgtggttatc cattgagtgg ctctggcgct
     1141 cgtgcatttt gcgagcgtag aacatcagga taatcaggct gacgatgaag gtacctgcca
     1201 gctcgaagga gcttgcgccc atcagcgcga tgaagcagaa cacacaaccc agtacagagc
     1261 agatcaggct gacaaagttg cggatgttaa cgccttcaaa acgaatcagg tcaacgcaag
     1321 agtagaagta cggcagcata gtcagcagta ctgcgatacc ggtcagttca ccgaacaggt
     1381 cagatgcttt accaccggca gagttcatca gagtgatgag gatcatcagg gcagtcattt
     1441 tcactgcagc cagcagcagt aatgccgatc agttaaggat c
//
LOCUS       DAZRUF010000411         1477 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZRUF010000411 DAZRUF010000000
VERSION     DAZRUF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.414"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..940)
                     /locus_tag="ACHXOL_004299"
     CDS             complement(<1..940)
                     /locus_tag="ACHXOL_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR6811093.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQH"
     gene            complement(966..>1477)
                     /gene="rfaY"
                     /locus_tag="ACHXOL_004300"
     CDS             complement(966..>1477)
                     /gene="rfaY"
                     /locus_tag="ACHXOL_004300"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR6811094.1"
                     /translation="FAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPNDFYF
                     LAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGDPHR
                     GNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRK
                     FIKKLKGKA"
BASE COUNT          455 a          314 c          259 g          449 t
ORIGIN      
        1 catgttgata tttcacttga tactgctttt cattcgtggc tggaataaac gccacatcat
       61 tccagcaaga agcctgataa gcctcattaa aatacttctg gcagggataa ttcgtccagt
      121 cgttccaggg cttcgttacg ccaacataat gaatcagctt agtatcatcg gtaatggtct
      181 ttttataatc ttcatcgctt ttatttttta actcataatc gatgccataa aagcaatcat
      241 aatcgccgct aataaaaata caatgaccaa agaacagaat atttaaaata tcctgatcaa
      301 ggcatttata cttcgtgtgc tgcgggtcac ttgccagcat agcaatggct ttattcataa
      361 aatcattttt cgcccatgct tcaagctgca gatatactac accggcgttg aaataattcc
      421 ctggaagtcc ctctatagcc agacgcttcg ctggtttttc ctgcataaaa ggcacatctt
      481 taataacagc tgcaaactta tccccatcaa aattaatatc aagcaatcca gctaatgagc
      541 ctttacagat gacatcagca tcgatatata acagattttc aatagtttca ctcagatact
      601 ggaacgcaac taaacggaag aatgttgcat aagaccactg tttcaggctg gggaagatat
      661 caataaaggc actggagact ttataaattc ttacttcaac gttagcattt ttgctggtaa
      721 aagcattaac acgttgtaga taatcttcat caatgtagtc ggtaaagaga tgaaacttaa
      781 ccgcaaggtc cggattatgc ataacaactg attgcataga tactgccgca ccaaataaaa
      841 agttacggtc aataccgtaa gcgatattta acacacgatc ggtgttagca atttccccag
      901 agacagcgac ttcagtaata ctctcagcta ataaatccat gttggttccg ttttgactgt
      961 gtggtttacg ctttgccttt taatttttta ataaatttgc gtagtttggt tctgtagatt
     1021 actgaatagt agccataatc ttttatttcg ttggcaatac ccaggtgacg ttccatggcc
     1081 agacgatcac gagctttacg ctctgccgta cagcttttac cggataggtc aattatcctg
     1141 acaccatctt tactaacgat aaagttaccc cggtgtgggt cgcccgatag catatttaag
     1201 gcatgcaatt tttccattga agccttaatt tctgctttaa catcttctgg aatgatcggc
     1261 atatcattga gctctacgcc ctcgacatac tcgataagca tgataaaaac actggcatag
     1321 ttgaaaattt tacgctcagc cagaaaataa aaatcattag gaaaagtaag acctgcactt
     1381 ctgacacgat cggtttcaac gatcaaattc tgatagtagt caccttttac aaaagatttt
     1441 aaaaaacgtt cattcctttt cgttttcggg gcaaaga
//
LOCUS       DAZRUF010000412         1457 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZRUF010000412 DAZRUF010000000
VERSION     DAZRUF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.415"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            99..1076
                     /gene="epmA"
                     /locus_tag="ACHXOL_004301"
     CDS             99..1076
                     /gene="epmA"
                     /locus_tag="ACHXOL_004301"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR6811095.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1295..>1457
                     /locus_tag="ACHXOL_004302"
     CDS             1295..>1457
                     /locus_tag="ACHXOL_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR6811096.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
BASE COUNT          346 a          333 c          373 g          405 t
ORIGIN      
        1 agtcactgcc caccgctgtt tgattcctgc gtaaaatttg tctcgccgtc gcgttgcgag
       61 tagacttcgt gcccttgtca aaaactggag atttaactat gagcgaaacg gcatcctggc
      121 agccgagcgc atccattcct aacttattaa aacgtgcggc gattatggcg gagatccgtc
      181 gtttctttgc cgatcgtgga gtgctggagg tggagacgcc ttgtatgagc caggcgacgg
      241 taaccgatat tcatttggtc ccgtttgaga cacgtttcgt tggccccggg cattcgcagg
      301 ggatgaatct ctggttaatg accagcccgg aataccatat gaaacgcctg ctggttgccg
      361 gttgtgggcc ggtattccag ctgtgccgca gcttccgtaa tgaagagatg gggcgttatc
      421 acaaccctga gttcactatg cttgagtggt atcgaccgca ctatgatatg taccggttga
      481 tgaacgaggt ggacgatctc ttacaacagg tgctggactg cccggcagca gaaagccttt
      541 cttatcaaca agctttcttg cgttatctgg aaattgaccc gctctctgcc gacaaaacgc
      601 aactgcggga agtcgcagcg aaactggatt tgagtaacgt tgctgatacc gaagaagacc
      661 gcgacacgtt gctacaattg ctgtttacct ttggcgtaga gccaaatatt ggcaaagaaa
      721 aaccgacctt tgtgtaccac tttccagcca gccaggcatc actggcgcaa atcagtaccg
      781 aagatcatcg ggtcgctgaa cgctttgagg tttattataa aggtattgag ctggcgaatg
      841 gtttccatga attaacggat gcccgtgagc agcaacaacg ctttgaacaa gataaccgta
      901 agcgcgcggc gcgcggtttg ccgcagcacc ccattgacca gaatctgatt gaagccttga
      961 aagtcggtat gcctgactgt tccggcgtgg cattaggtgt tgatcgtctg gtgatgttgg
     1021 cgctgggcgc ggagacactg gctgaagtca tcgcctttag cgttgaccgg gcataattct
     1081 gaaaaattac tgaattaaca gcgaagaatg gcgtgataag cgccattctg tagcatatgt
     1141 tttcattttt ccgttaaagg ccacaattta agactggata tttgatatca tccgggtatc
     1201 aattctgtat tggtttttac gctgcctgct caatgttgcg cagtgcagtt cagtgggcac
     1261 gtgttataca cgcgctgaaa tgaaggatgg tttcatgcct cacacgataa aaaagatgag
     1321 tctgatagga ctcatattga tgatctttac ttccgtattt ggatttgcca atagcccatc
     1381 ggcttattac ttaatgggtt atagtgcgat tcccttttat atattttctg cattgttatt
     1441 ctttattcca ttcgcct
//
LOCUS       DAZRUF010000413         1437 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZRUF010000413 DAZRUF010000000
VERSION     DAZRUF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.416"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..493
                     /locus_tag="ACHXOL_004303"
     CDS             <1..493
                     /locus_tag="ACHXOL_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR6811097.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="ACHXOL_004304"
     CDS             493..1083
                     /locus_tag="ACHXOL_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR6811098.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1070..>1437
                     /locus_tag="ACHXOL_004305"
     CDS             1070..>1437
                     /locus_tag="ACHXOL_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6811099.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMT"
BASE COUNT          371 a          318 c          420 g          328 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgac
//
LOCUS       DAZRUF010000414         1428 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZRUF010000414 DAZRUF010000000
VERSION     DAZRUF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.417"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHXOL_004306"
     CDS             complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHXOL_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR6811100.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHXOL_004307"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHXOL_004307"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR6811101.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1428)
                     /locus_tag="ACHXOL_004308"
     CDS             complement(822..>1428)
                     /locus_tag="ACHXOL_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR6811102.1"
                     /translation="KGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFC
                     FGKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGAD
                     EEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVP
                     GAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          324 a          397 c          396 g          311 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgccttta
//
LOCUS       DAZRUF010000415         1427 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZRUF010000415 DAZRUF010000000
VERSION     DAZRUF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.418"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..655)
                     /locus_tag="ACHXOL_004309"
     CDS             complement(<1..655)
                     /locus_tag="ACHXOL_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFR6811103.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
     gene            complement(819..914)
                     /gene="ymiC"
                     /locus_tag="ACHXOL_004310"
     CDS             complement(819..914)
                     /gene="ymiC"
                     /locus_tag="ACHXOL_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR6811104.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1028..1195)
                     /gene="yciX"
                     /locus_tag="ACHXOL_004311"
     CDS             complement(1028..1195)
                     /gene="yciX"
                     /locus_tag="ACHXOL_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR6811105.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1198..1338)
                     /locus_tag="ACHXOL_004312"
     CDS             complement(1198..1338)
                     /locus_tag="ACHXOL_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR6811106.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          383 a          348 c          331 g          365 t
ORIGIN      
        1 tgatcatagt ggtgtgcgag tcagtaccaa cgagtgtatc cggataagca atccattcac
       61 cgtcctgcaa ttcactccac actgctttgc cgagatattc gaggttaacc tgatggcaaa
      121 tgcctgtgcc tggcggcacg acgctaaacc gactgaacgc ttgctttccc catttcagga
      181 acacataacg ttcgtggttg cgctccattt ccaggcgtac gttttcttca aatgcctcat
      241 catcaccaaa acgatcgacg gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg
      301 ggttaacctt tgcagtatcg ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat
      361 caacaacggc aggtacgccg gtaaagtcct gcatcaacac ccttgccggg cggtaggcaa
      421 tttcacggtc agcatgggca tttttcagcc atcctgccag cgcgtggata tcctcttcgg
      481 taaccgagtt accatcctgc cagcgcagca ggttttcgag caaaactttg agtgacttgg
      541 gtagacgggt gatatcgccc agtgatttag cagcaagcgg caggctgtag tagtggtaag
      601 ttttatcttt ggcctgcaac gtgtccttac tggcttctcg tagggttgac gacatagctc
      661 ctccttaatg acagggttgc gaagccctga ttctcttcag ggtgaatatt aaagataaca
      721 caaacatcgc gtaacgattt gataacaacc caaattgata aaagaggaga aaccttgtgg
      781 caaagcaaat gacaaccccg ccgcagcggg gtcaaggatc agtgagtaat gatccagagg
      841 agttcggccc agaagagcat cgacagagaa aacgcgccca tccaggacca atatttcata
      901 tttgtgttga tcataggcta cacccttaac ggttattatt ttgtgttgag ataactcaag
      961 ctgttattgc tgcggtgtat taaaagccct ttaattcatt aaaaatgggt aataagaatg
     1021 atgataatta ttgtttcggc tgatcatctt cagcaaacag ttctataaag gcttgctgct
     1081 gcgatgttag ttgccagtcg tctggaataa cgacggaaca ttcccgtttc gtttctgttt
     1141 cattgtcact atgctggcat aatggtgacg actccagttg ctcctgaccg accatagtta
     1201 tccccacact ttccaaatca gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa
     1261 aaccgccagc caggctttac gttttaattc aggatcgcgg cggggttcct gatttccaga
     1321 aggcattgct aacctcatat aatcgccatc acttatcagc aagacgttga acgattggtg
     1381 taattaatca tacgatgaga caaatcctaa agaaacttta gtcaaaa
//
LOCUS       DAZRUF010000416         1425 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZRUF010000416 DAZRUF010000000
VERSION     DAZRUF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.419"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(84..881)
                     /gene="mtfA"
                     /locus_tag="ACHXOL_004313"
     CDS             complement(84..881)
                     /gene="mtfA"
                     /locus_tag="ACHXOL_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR6811107.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            975..1064
                     /locus_tag="ACHXOL_004314"
     tRNA            975..1064
                     /locus_tag="ACHXOL_004314"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1009..1011,aa:Ser,seq:cga)
BASE COUNT          407 a          328 c          314 g          376 t
ORIGIN      
        1 catattactt agcggtacct tgtctgtcaa cactaaattt cataagtcat ttcaattggt
       61 taattaatct gcaaagttgt taattaatga acattcgtcg ccgaaaacga gtctgtatca
      121 ttagcgtgat gcagtctctg caaaggatct tgttgataaa attggcagaa acgttgccac
      181 aatgaaggga aacgaggagc aaaaagttct ggggcgctaa agaaatattc agaaagtacg
      241 gcaaaacatt cagcaggatc gctggctgca taagcatcaa tgctcgccgc attctcgcca
      301 accaattcga tttcttcctg aatgttgttc attgcagcat gaagatcgtg ttcccagcca
      361 gcaacctcac gcaacggaat aaagggaact ccgctggcgc gatcgccgtt acgggtgtcc
      421 agcttatgag cgacttcatg aataatcagg ttaaaaccag atgcatcaaa agaatcttgt
      481 atatccaacc agttcaaaac gataggccct tgctgccagc tctgacctga ctgaacaata
      541 cgttggttat gcaccagacc gatatcgtct tcccattcat catcgaccac aaatggcgca
      601 ggataaatta agacttcatg aaaaccatcc agccattcca gtcctaactc cagaacgggt
      661 aggcaaaata gaagtgctat ccggcagctt cttaatgaat ccagttcaaa gccctgtaaa
      721 ggaacaagcc gcttttgctg taaaaaacgt tcggcaagag tgactaattt gctttgttcc
      781 tgttccgtca gacccgttaa aagggggatc gatagtgctt cctgccaggg aagggcagtt
      841 tgatgtgctg attcttgtac tttccagggc cacttaatca tcgttttgct cgcaaactcg
      901 tcacttgaac aaaattgcac ggacagggac tgttaaaatg ccaaatttcc tggcatcatg
      961 gcaaccatct gaacggagag atgccggagc ggctgaacgg accggtctcg aaaaccggag
     1021 taggggcaac tctaccgggg gttcaaatcc ccctctctcc gccactttat caatgactta
     1081 tctcctgact tcccgccttg cttttcctaa acagaacaat tgtagaatat tcttgaaggg
     1141 ttagatcgtc actgttttct gttcgatact gtgacattca gcacttgatt cgctatggat
     1201 ctgacaggaa gctttcgagc gaaaatgtgc agttattcag tcgttttctt atcgctcacc
     1261 attattcttt tagacattca tcctatgaaa ctgccgcaaa gttggtggtg ggaactgaag
     1321 ttgcgtagag aaggggtcaa taattgagtc aaaaaaggaa cgcggccgtc agattaaagc
     1381 cgcgggacaa agtccataat ttccaagagt tgttgaattt cccag
//
LOCUS       DAZRUF010000417         1424 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZRUF010000417 DAZRUF010000000
VERSION     DAZRUF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.420"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            147..698
                     /locus_tag="ACHXOL_004315"
     CDS             147..698
                     /locus_tag="ACHXOL_004315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811108.1"
                     /translation="MNIDNWAIPITSLGGQLLGVELVSHVEREGIRQIGLWEEEVLHG
                     QLMLIESKAAWFRDNGLYCVITTGRGEPYEYLPFLRQMTRDMKVHNQQWLDDLGVHGG
                     SAIPLVTGDFEVARLNRRYTEENIDRLIFPVLIKSIRQYCEKVIVPVRDKTYYPILRE
                     AGVWAVQGEFRPMRFEQCEKLIG"
BASE COUNT          426 a          257 c          357 g          384 t
ORIGIN      
        1 gtcagtttcc caaaaatcca gcaccccttt gtttaccact ttgaatgatt aattctattg
       61 taatgatcaa taaaattgat cgatttaatt gccaatgttt taaaaatgat caattatcaa
      121 taacttaaag cgtaacgagc aaagttatga acattgataa ttgggcaata ccgataacaa
      181 gtttgggtgg tcagttgctg ggggttgagt tggtcagcca tgtagaacgc gagggaatca
      241 ggcagatcgg cctatgggaa gaagaagtac ttcatgggca actaatgctt attgaaagca
      301 aggcggcatg gttcagggat aacggtttgt attgtgtgat tactactggc cgtggtgaac
      361 catacgagta cttaccattc cttcggcaga tgacaaggga tatgaaagta cataaccagc
      421 agtggcttga tgatctcggt gtacatggtg gttctgctat tcctctggtt acaggtgatt
      481 tcgaggttgc ccgattaaat agaaggtaca cagaagagaa cattgaccgt ctgatctttc
      541 cagtactcat aaaaagcatt cgccagtact gcgagaaggt gatcgtaccc gtcagagata
      601 aaacgtatta cccaattctc agagaggccg gtgtatgggc ggtacaggga gagttcaggc
      661 caatgcgttt tgagcaatgt gaaaaattaa taggataaca ataatgaaaa aattactaat
      721 tgggttactg attgccctta gtactggatg tactactacg cacagcttca tggataaagc
      781 ttacgttggc tatgtagatg acaaggccgt agtactgttt gatgcaggaa cacgtaatgg
      841 ggtgaagtac taccgtcctc agttccagag tgattgcggt cgcatctata acaagatcta
      901 tcatgatgac aatacaagta ctggatgcct catacgagat tcccgcattg ctgaactaca
      961 tccctataga gagcatgtaa gtaaggtagt accacatcag accgatagtg ttcacgattg
     1021 gttagaaggg gtcaattcac catcccatat cctcgacaat ctatagggta cgtatgggaa
     1081 aggtaatggt gattttgtta gtactggctg gggtactctt caacattgca ggtgaggatg
     1141 caccccagcc acagtacacg gacaatgaga accagaacgc tatggatttc gcacagtcac
     1201 taatcaaggg taagtaccca tgcgatatgg tgactatgga tagtactgag aatggtacag
     1261 gacgtattga tgtacggtgt gatggtaagt accagtatga gatcatccgg catgatggac
     1321 aatggcaggt agtatcgaag taatccgaaa agtttagtgc ttaggctgta tggtgagaat
     1381 ctacagggct ttttctttat gcggtgataa gatcggagtg aact
//
LOCUS       DAZRUF010000418         1421 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZRUF010000418 DAZRUF010000000
VERSION     DAZRUF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.421"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>1421
                     /gene="rhsC"
                     /locus_tag="ACHXOL_004316"
     CDS             <1..>1421
                     /gene="rhsC"
                     /locus_tag="ACHXOL_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFR6811109.1"
                     /translation="LVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIR
                     LSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVA
                     HRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAG
                     LKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRAS
                     AFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATE
                     DATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQ
                     LIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYD
                     AAGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTH
                     WHYDEADRLTHRTVNGETAERWQ"
BASE COUNT          338 a          422 c          436 g          225 t
ORIGIN      
        1 ctggtactga ccacgcaggc gcagcgggca gaagaagccc ggcagcaggc catttccggc
       61 gggacggaac cgtccgcttt tcctgatacc ctgccgggtt acaccgaata tggccgggac
      121 aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc cggaataccc ggagaattta
      181 cctgccgcgc cgctggtgcg ctatggctgg acgccgcgcg gcgaactggc ggcagtgtat
      241 gaccgcagca atacacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg
      301 gtggcgcacc gtcacacggg ccggccggaa atccgttacc gttacgacag cgacgggcgg
      361 gtgacagaac agctaaaccc ggcaggctta agctacacgt atcagtatga gaaggaccgc
      421 atcaccatca ccgacagcct gaaccgccgt gaagtgctgc acacgcaggg tgaagccggg
      481 ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag tcagtttgat
      541 gcggtgggca ggctcagggc acagacggat gccgcaggcc ggacgactga gtacagcccg
      601 gatgtggtga cgggactcat cacgcgcatc accacgccgg atggcagggc atcggcgttt
      661 tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct ggaaatgcgc
      721 cgcaaatatg atgaatccgg ccgtctgatt caggaaactg cccctgacgg cgatatcatc
      781 cgctaccgtt atgataatcc acacagtgac ttaccctgcg caacggaaga tgccaccggc
      841 agccggaaaa ccatgacgtg gagccgttac ggtcagttgc tgagcttcac cgactgttcc
      901 ggttatgtaa cccgttatga ccatgaccgc ttcgggcaga tgacggcggt gcaccgcgag
      961 gaagggctga gtcagtaccg cgcatacgac agccgtggac agttaattgc cgtgaaagac
     1021 acgcagggcc atgaaacgcg gtatgaatac aacgccgccg gtgacctgac caccgtcatt
     1081 gccccggacg gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc tatctgtacc
     1141 acgcagggcg gtctgacgcg cagtatggaa tacgatgctg ccggacgggt catccgcctg
     1201 accagtgaaa acggcagcca caccaccttc cgttacgatg tactcgacct gctgatacag
     1261 gaaaccggct ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc
     1321 cgcagcgagg atgaggggct ggtcacccac tggcactatg acgaagcaga ccgcctcacg
     1381 caccgcaccg tgaatggcga aaccgcagag cggtggcagt a
//
LOCUS       DAZRUF010000419         1412 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZRUF010000419 DAZRUF010000000
VERSION     DAZRUF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.422"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..251)
                     /gene="dinJ"
                     /locus_tag="ACHXOL_004317"
     CDS             complement(<1..251)
                     /gene="dinJ"
                     /locus_tag="ACHXOL_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR6811110.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDK"
     gene            467..1210
                     /locus_tag="ACHXOL_004318"
     CDS             467..1210
                     /locus_tag="ACHXOL_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR6811111.1"
                     /translation="MPSIPSFVLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRA
                     RLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALA
                     ENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAK
                     LPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
                     TIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT          350 a          340 c          345 g          377 t
ORIGIN      
        1 aatttatcaa ataaatcatc agcgtctttg gccttatgaa catcaacgcc agcttcgctg
       61 tttttgattg attgaatggt taattgatta ggctcgcgta aatcaaacgg caatgccttt
      121 tcacgcgcga cctttgtgag ggttatgcga accaggtcag agatggtcag ccccatcccg
      181 gccagtacgt ccgccgcctg attcttcaga tcttcatcga ttcgggcgcg aacaaacgcg
      241 ttagcagcca ttgggggatc tcctgtgctg tatatttatt cagcttgaat tgtagctcaa
      301 atgagcaaca gcgacaatcc atgagtaatt taataaacgg atgtttttac ataataaact
      361 gaaatatatt tattaatttt ataataatgg tttattaagt ttgttcaggt acgggccagg
      421 atacactgca acatcaatgc atgaccagcc attattgaga atgtctttgc cgtcgattcc
      481 ctcttttgtg ttgtcgggat tactgttgct ttgcttgccg ttttcttcat ttgcctgcgc
      541 caccacatca catatctctt tcagctacgc cgcccgccag cggatgcaaa accgtgcgcg
      601 tttattaaaa cagtaccaaa cccatctgaa aaaacaggcc agctacattg tggaaggcaa
      661 tgcccaaagc agaagggcgc tgcgccagca caaccgggag cagataaaac agcatccaga
      721 atggtttcct gcgccgctca aggcgagtga cagacgctgg caggcgctgg cggaaaacaa
      781 ccacttttta agcagcgatc acctgcataa catcaccgaa gtggcgattc accgtctgga
      841 gcagcagctt ggcaagcctt acgtctgggg cggcacgcgg cccgatcaag gcttcgattg
      901 tagtgggtta gttttttatg cctacaacaa gatcctcgag gcgaagctcc cgcgtacggc
      961 caatgagatg tatcactacc gtcgggcaac cattgtggcg aataacgacc tgcgccgggg
     1021 agatttgctg tttttccata tccacagccg cgagattgcc gaccatatgg gggtgtatct
     1081 gggcgacggt cagtttattg agtcaccgcg caccggggaa accattcggg taagtcggtt
     1141 agctgaacct ttctggcagg atcatttttt gggcgcgcgg cggatattga cggaagagac
     1201 gattttgtag gacggataag gcgtttacgc cgcatccggc ggtggtgcgc agatgcctga
     1261 tgcgacgctg acgcgtctta tcatgcctac aaatctgtgc gtttttgcga gccgctttcc
     1321 tgttattcca tcacccaggg aaagatcatc agaagtacac tttttgtact aaataattcg
     1381 cattttatgt ttaaaatttg agatattcct ca
//
LOCUS       DAZRUF010000420         1404 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZRUF010000420 DAZRUF010000000
VERSION     DAZRUF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.423"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(301..>1404)
                     /locus_tag="ACHXOL_004319"
     CDS             complement(301..>1404)
                     /locus_tag="ACHXOL_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR6811112.1"
                     /translation="LAWAVFKIFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTF
                     TAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTA
                     THNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNF
                     LAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGY
                     VTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPG
                     QVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYA
                     QVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          365 a          382 c          324 g          333 t
ORIGIN      
        1 aatgtgactg tcatccttca gataataaag ccatcatcgc aatagatgga aaagcactcc
       61 tgctcttttt tgattagagc cgtcgcagga ggaacaactc atatcatcat tgcgatctcg
      121 acgatgtata gtctcatcct gcggcagaac aagacggata aaaaatccaa tgagagcaca
      181 ctcatccaga acttattaac ataatgaata ttaaagaaaa atcatcgcag ccaatgaagt
      241 aatatgcaaa aataattagc ccttgcaggg agcaaagttc cataatctcc cccttccccc
      301 tcaatgatcc aaatacaaat aatgcatcca actggtcatt cttagcagca ccttccgcat
      361 caccgaaaca tggctgaaat ggtcggagta aactgctacc tgggcgtaaa tgccgtcggg
      421 taaggcatcg atatcatcgt tagggtccag ttcaatggtt cccagcacgc cgtccgtgcc
      481 gggaacgacc gttaatgatt gcaatacccc ctgcgcctga taagaaccgc ctggcacgac
      541 aggtaaaata ctagtcaatt tgccgtggaa cacctgccca ggtagcgcgt taaacaccac
      601 ttccgcatca tcgccaggtt tcagacgtaa cagcgagttt tgccgaaatt gggcgacaat
      661 ttgccgtttt tgctcgggga tgaagaccat caccggacgc agcggcaagg cagctgcgta
      721 tgtacctggg cggatcagta cctgagtaac gtagccattg ctcggcgcgc gaatgacagt
      781 ctgctcaagg ttatattttg cttcagtaag ttgcgctctt aagctcacaa tctgcgattg
      841 ctcgccgtta accatactgt cgagctggct ctggatctgc gcctgctccg ccaccgagcc
      901 tttcaccagc gcatcctgcg cgaggaaatt ttgccgcgca tcgtcgatgt cacgttccga
      961 gaacggattc accgccgcct ggctgccttt caggtaacgt tgataatttt taaacagacg
     1021 gtcgcgctcc gctgaaacct gggtggtgtt ggcctgcgct tcagtgagct gcgcacgcag
     1081 cgtctttata ttatgcgtcg ccgtcatcag gtcagcctga agtctgtcaa ctcgcgcctg
     1141 gtaacgaacc gggtcgagct taaaaagcac ctcgcccttt tgaataagct gattattctt
     1201 gtcagtgact tcagtaacaa ttcccgtcac ctgtggcgtg atagggatcg ctatcactgc
     1261 cttttgcgcg gtaaaagtgt aagggtggtt gtagttcatc aacaaaatca aaccactcac
     1321 cagaaacacg cctcccagcg ccgccgtcgc cagcgtccac tgattcaccg gaatacgaaa
     1381 gattttaaag accgcccacg ccag
//
LOCUS       DAZRUF010000421         1394 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZRUF010000421 DAZRUF010000000
VERSION     DAZRUF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.424"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..461
                     /locus_tag="ACHXOL_004320"
     CDS             <1..461
                     /locus_tag="ACHXOL_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR6811113.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            479..1117
                     /locus_tag="ACHXOL_004321"
     CDS             479..1117
                     /locus_tag="ACHXOL_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR6811114.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          445 a          283 c          307 g          359 t
ORIGIN      
        1 cattgaatgc aatggcgtcg caacgaacca aagaggctcg tggtggagaa ttacagatca
       61 acgaattact tcccgccagc ctggatgcag caaaagccca tggactgacc gaacgcgatg
      121 tttatgaagc aactgactac tataaaacac cacgcgggca acaaccggga ggtgcgacaa
      181 aaatgttgtt ctcacatgca cagaaaacgc tggcctggga tgccttcgca tttactgaag
      241 tgttactgac tcagccggtg atggtagtag ttggtgaaaa agtcggtgcc ttcggtgctt
      301 atcgtgatgg actggaagtc tatggccggg cgatggtttc gcaggatcgt cagttagttt
      361 cattacccga tttctcacat tatgaactgt atgacaaacc ggaagcagtt caggaggcgc
      421 tggaaaaagt tattcctttt ttcaacaccc atctgggata agtttcatga gaaatattat
      481 gaaaaatgta ctgattcttg gtgctggcgg tcagatagcc cgccatgtga ttaatcaact
      541 ggcagataaa caaaccatta aacaaacttt atttgcccgt cagccagcaa aaatccacaa
      601 accctatcca actaatagca agattattat gggagatgta ctgaatcatg cagcactgaa
      661 gcaggccatg cagggacagg acgttgtgta tgccaacctt acgggggaag atttagatat
      721 tcaggcaaat tcagtgatcg ctgcgatgaa agcctgtgat gttaaacgtc tgatctttgt
      781 tctttccctc ggtatttatg atgaagttcc aggaaaattt ggcgaatgga ataatgcagt
      841 tattggtgag cccctaaaac cattccgcag ggctgcggat gcaattgagg cttctggact
      901 ggaatataca attcttcgcc ctgcctggct gactgatgaa gatattattg attatgagct
      961 tacctcacgc aatgaaccat tcaaaggtac gatcgtatca cgtaagagtg tcgcagcatt
     1021 aatcacagat atcatcgata aacctgagaa acacattggt gagaatatag gtatcaatca
     1081 acccgtgact gatggtgaca agccattatt catgtaaacc aattaaatag ttaccatcgt
     1141 aatgtactat gtgcagtacg atttattaaa cttattaagg tgaactgatc aatccgatgc
     1201 gattaacatg tgtagaacca gtaagaataa aatacgccat gtatataaca ttacgactta
     1261 catctaagtc agcataattc taatatctgc acatcaataa aaatgcaaac aacttccggc
     1321 aaaaaaagtg atacaagatc gatgacatag cgttgaagaa aaggatttga taatccgcaa
     1381 aatttgcaca acac
//
LOCUS       DAZRUF010000422         1384 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZRUF010000422 DAZRUF010000000
VERSION     DAZRUF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.425"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..1339)
                     /locus_tag="ACHXOL_004322"
     CDS             complement(<1..1339)
                     /locus_tag="ACHXOL_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR6811115.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAZRUF010000423         1377 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZRUF010000423 DAZRUF010000000
VERSION     DAZRUF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.426"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..984
                     /locus_tag="ACHXOL_004323"
     CDS             <1..984
                     /locus_tag="ACHXOL_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR6811116.1"
                     /translation="LVVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVR
                     SIDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTP
                     PGYTGMGEPLPVLYFYHSFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETD
                     AKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQ
                     GGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGD
                     KDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            999..>1377
                     /locus_tag="ACHXOL_004324"
     CDS             999..>1377
                     /locus_tag="ACHXOL_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR6811117.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          360 a          315 c          362 g          340 t
ORIGIN      
        1 ttagtggtgg gtgttccggt tccggacaat attcacccga tgatcaaaga cgaagcagga
       61 gtctggtcgt ggcgcacgcc tgtcctgaaa ggcaacctgt atgagtattt tttcaatgtt
      121 gatggtgtac gcagcattga tataggcacc gcaatgacta agcctcagcg ccaggttaac
      181 tccagtatgg ttctggtgcc aggcagttat ctggatacgc gttctgttgc gcatggtgat
      241 ttgatcgcca taacttacca ctccaacgca ttgcaatctg aacgtcagat gtatgtctgg
      301 accccgccag gatacaccgg catgggcgag cctttgccag tgctctattt ctatcacagt
      361 tttggtgata ccggacgttc cgctatcgat caggggcgta tcccgcaaat catggataac
      421 ctgctggctg aagggaaaat taaaccgatg ctggtggtga tcccggatac cgaaaccgat
      481 gcgaagggca ttattcccga agatttcgtg cctcaggaaa gacgtaaagt cttttatccg
      541 ctgaatgcta aagcggcaga tcgcgaactg atgaacgata ttatcccgct gattagcaag
      601 cgttttaatg tccgtaaaga tgccgatggt cgcgcgctgg cagggctttc acaaggcggg
      661 taccaggcgc tggtttccgg aatgaatcat ctggaaagct ttggctggct ggccacattc
      721 agtggtgtta ccacgacaac cgtaccggat gaaggtgtcg cggcccggct gaacgatccg
      781 gcagctatta accagcaact acgtaatttt actgtggtcg ttggtgataa agatgtcgta
      841 accggcaagg atatcgccgg gctgaaaact gagcttgagc agaaaaaaat taagtttgat
      901 taccaggaat atcccggcct gaaccatgaa atggatgtct ggcggcctgc ctatgcagcc
      961 ttcgtacaga aattatttaa ataaaataag gcattaagat gaaattaatc attaccgaag
     1021 attaccagga aatgagccgt gtcgcggcac accatctgct tggttatatg tcgaagacgc
     1081 gtcgtgttaa cctggcaatt accgccggta gcacgcccaa aggcatgtat gaatacctca
     1141 ccaccctggt taaaggtaag ccctggtacg ataactgcta tttctataat ttcgatgaaa
     1201 ttccctttcg cggcaaagag ggagaaggcg taacgattac caatctgcgt aatctgtttt
     1261 tcacccctgc ggggatcaaa gaagagaata tccagaagct cactattgat aactaccgcg
     1321 agcatgatca gaaactggcg cgtgaaggcg gcctggattt ggtggtgctg ggattag
//
LOCUS       DAZRUF010000424         1371 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZRUF010000424 DAZRUF010000000
VERSION     DAZRUF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.427"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..185)
                     /locus_tag="ACHXOL_004325"
     CDS             complement(<1..185)
                     /locus_tag="ACHXOL_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR6811118.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
     gene            complement(230..1216)
                     /locus_tag="ACHXOL_004326"
     CDS             complement(230..1216)
                     /locus_tag="ACHXOL_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR6811119.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT          378 a          301 c          278 g          414 t
ORIGIN      
        1 gcacctaata accctaccca gatcatcaaa aatcgtgcca gctcatcggt taatgtactt
       61 ggctggttaa gtacgtagcg gctaaatact tgccagacta cgcatacaac cagagcaagc
      121 atgacggcga cagaaaacgc ggcaatgacg cggtctaccg caagtttcag gcggttaaat
      181 atcattgtaa gcatcttgga aaatcagggc cagagactgg cccggaatat tattgagcag
      241 cgttgtcgaa cttctctaac aaagcagctt gttttggatc ttttttgaag tcgtcaaaca
      301 gaggctgaac tgcggcacgg aatggtgctt tatccacttt tacaatttca ccccccatag
      361 ctttcgcctg agcacgtgtg tcagcgtcaa ttttttccca caatttttgt tgataagctt
      421 cggatttctt cgctgctgtt tcgaggattt gtttctgttc tggggttaat ttattccatg
      481 ttttggtgga gataaccaaa aagtcaggaa ttgaggcgtg ttcatcttca gaaaaaactt
      541 tggcaatttc aatatgtcga gtctgcaccc aggaaggcac gttattttct gcgccatcta
      601 ctacaccttg ctgcatcgca gtataaactt cgccaaatga aattggagtg ggtgaaccac
      661 ccatcagttc aatcattttg atggtggtcg gacttggctg aacacgaatt ttcagccctt
      721 taagatcttc tggcttagta atcggttttt tcgcatagaa actgcgtgta cccgctacat
      781 aagctgataa agcaaaaaag cctttgtctt ttgttgagtc cataatttct ttaccaactt
      841 caccaaaaac aactttgttg aagtgagcct gatctttaaa taagaacggc aaattataga
      901 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
      961 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1021 tacggatcac catattacct tctgacagct gttttacctc tttcgccagc tcttcaaaac
     1081 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgtc actttttctg
     1141 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1201 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
     1261 tgtgcttatc agtgctttgt gcgacaaatt ggttaacctg tctgacaact tgtctatgtt
     1321 tatcacaact tgaaataaag tcagaaatta aatattaaat ttgtgcgcca g
//
LOCUS       DAZRUF010000425         1364 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZRUF010000425 DAZRUF010000000
VERSION     DAZRUF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.428"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..1259)
                     /locus_tag="ACHXOL_004327"
     CDS             complement(<1..1259)
                     /locus_tag="ACHXOL_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR6811120.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQSSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYT"
BASE COUNT          380 a          348 c          304 g          332 t
ORIGIN      
        1 gtgtaaccta ttgcgttgtt tgtattacgg tattcggcaa cgactttaat cattccctcc
       61 atcatacttg ccacttctgt ttcctgcggc gagatcatgc ggacattttt catgacctgg
      121 gattgcatca ccgtctggct accagagtct tccgggcgtt gccaggtctg gatctcctga
      181 tcgttaccgc caacagtacg ccagttggta attgcaccac tgaagatgtc acgcacctgt
      241 tgttcagtca gggaattaac cggattatcc gcattgacga tgaaaacaaa cgcttcacgg
      301 gcaaaaggcg tgtacagcaa agtaacgccc gattcctcag cgcgtttttt ctgcccaccg
      361 gaagattgcg ccacaaaaat aatatcagca tcacctttaa caatcctgtt atacgcatcg
      421 ggggtacgag agctctccag atattccctg gtgtgaaaat cttccggtat aacgcttaaa
      481 gcataaaatg cagaggcata aattgggtac gccgccgtag caccatcgag tcgcggccag
      541 ttttgcgtaa actgaatttg cggtttacca cgcagtggtg tcagttgatt attgagttta
      601 tcgggtcgcc aggcccaggt atcaatattt tcgcgcacac cgacaccggg attgagctgt
      661 ttatataaat aaccctgata accagcgaca aaaccaagca tgagaaggat aaacaggagg
      721 gaattacggc aacgtagccc ggatgtaccg gttaaaggat gtctggttgg ccggccgtac
      781 cccagcgaaa aactgatatg cgcgccaacg ggtataatga ctatccacca gtactcacct
      841 ccgactactg agactaacaa aacgcccagc caggggagca taagatcagc caaccaaccc
      901 gatcccatct ggtgaaattg ataatctgcc agatgcagcg cgattatcca gccaaccagg
      961 attaataaac cgggtactaa aaaaagacat tgtcgccaga cggctatttc tcttttttct
     1021 ggtgcactat cgcctgccca tactcgcccc gagaaaaata gcattaatgc gcccagcgcc
     1081 agcataaaaa tgttgagcag aaggtaactt gttggcgtga taaggatatt acaaacttca
     1141 ataataccgg caagtccgac acccagcata gcaacaaccg ccgccaaaaa ttgcgtcagt
     1201 atggggatgc cgaaaaaagt cattaccagc gaggacaaaa tccattttct gttttgcatt
     1261 attctttcca ttctttttga atggtgaaat tatactcccc gagtcccctt gccccttctg
     1321 gacacttttc cgaaatgatg gcggaaaaaa acgggaccct ttgg
//
LOCUS       DAZRUF010000426         1341 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZRUF010000426 DAZRUF010000000
VERSION     DAZRUF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.429"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHXOL_004328"
     CDS             <1..184
                     /locus_tag="ACHXOL_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811121.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="ACHXOL_004329"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="ACHXOL_004329"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR6811122.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAZRUF010000427         1340 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZRUF010000427 DAZRUF010000000
VERSION     DAZRUF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.430"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            334..>1340
                     /gene="shiA"
                     /locus_tag="ACHXOL_004330"
     CDS             334..>1340
                     /gene="shiA"
                     /locus_tag="ACHXOL_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR6811123.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
BASE COUNT          287 a          323 c          330 g          400 t
ORIGIN      
        1 acaatgatgc taaaaacata cctaacctcg cctccctact ggttataatg caatgcagtc
       61 tatcagactc atcagggtgc cattttgtgc atatgcggac ttttatgttt catatctcta
      121 acctgtgggt cctctgctta atccttaaac aacaccagca actcctgcgc tttcatcttc
      181 catcgaattt ttcatgttgc cgctaatcag ccataaaatc atttgcagat gcgctctgtc
      241 gaggtagtct cataaggttc gtttatagat cgacggcaat gtgagttacc ttttccatac
      301 taattataaa aagacagtac aaacaggatc attatggact ccacgctcat ctccactcgt
      361 cccgatgaag ggacgctttc gttaagtcgc gcccgacgag ctgcgttagg cagcttcgct
      421 ggtgccgtcg tcgactggta tgatttttta ctctatggca tcaccgccgc actggtgttt
      481 aatcgcgagt ttttcccgca agtaagcccg gcgatgggaa cgctcgccgc atttgctacc
      541 tttggcgtcg gattcctttt ccgtccgctc ggcggtgtca ttttcggtca ctttggcgac
      601 cgactgggac gtaagcgcat gttaatgctg accgtctgga tgatgggcat cgcgacagcc
      661 ttgattggta ttcttccttc attctcgacc attgggtggt gggcacctat tttgctggtg
      721 acactgcgtg tcattcaggg atttgccgtc ggcggcgaat ggggaggcgc ggcgttgctt
      781 tccgttgaaa gtgcaccgaa aaataaaaaa gccttttaca gtagcggtgt acaagttggc
      841 tacggtgtag gtttactgct ttcaaccgga ctggtttcat tgatcagtat gatgacgact
      901 gacgaacagt ttttaagctg gggctggcgc attcctttcc tgtttagcat cgtactggta
      961 ctgggagcat tgtgggtgcg caatggcatg gaggagtccg cggaatttga acaacagcaa
     1021 cattatcaag ctgccgcgaa aaaacgcatc ccggttatcg aagcgctgtt acgacatccc
     1081 ggtgctttcc tgaagattat tgcgctacga ctgtgcgaat tgctgacgat gtacatcgtt
     1141 actgcctttg cacttaatta ttcaacccag aatatggggc taccgcgcga acttttcctt
     1201 aatattggtt tgctggtagg tggattaagc tgcctgacaa ttccctgttt tgcctggctt
     1261 gccgatcgtt ttggtcgccg tagggtttat atcacaggtg cgttaatcgg aacgttgagc
     1321 gcatttcctt tctttatggc
//
LOCUS       DAZRUF010000428         1320 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZRUF010000428 DAZRUF010000000
VERSION     DAZRUF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.431"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..348)
                     /locus_tag="ACHXOL_004331"
     CDS             complement(<1..348)
                     /locus_tag="ACHXOL_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811124.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAI"
     gene            complement(351..1061)
                     /locus_tag="ACHXOL_004332"
     CDS             complement(351..1061)
                     /locus_tag="ACHXOL_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811125.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1077..>1320)
                     /locus_tag="ACHXOL_004333"
     CDS             complement(1077..>1320)
                     /locus_tag="ACHXOL_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR6811126.1"
                     /translation="RYYATQMNADKNIKLLAINGIAPTAENIRNGKYPYIVDAFMVTR
                     ENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT          351 a          317 c          293 g          359 t
ORIGIN      
        1 aattgcccgt tcatccgcac gccagttttc tttgttggca aggttgatac ggcgcagaga
       61 aaaaactacg tgaatattgt caaaatgccc ctgtgcaaca aattccagta aacgcggcag
      121 aaaatagcgg acctcatcgc tattttgcgg atcaggccct gggctactat tccaggcatt
      181 gattaaggaa tacgggatcg ctcgtaacga tgtactccgt aatgcctttt gttcctgctg
      241 actcaggcaa tattcacaac aaaccacaaa tttctgcggc acccgataac gacgaaaggt
      301 gtgataaatc tgctcaatta atgcctgcat ggcggggctg ggttgcatag ttaccccctc
      361 gcccgctgat cgcgtgtaaa cggttgatag acaaaacgag cattcgccgc gttcaggcga
      421 ataagggcgc tacgcgaaag ggaaaagctg gggcgaatgc aactattgca gtctcgacag
      481 ctttcctctg cgcaattttc cactgagtaa tgacgcgtgt agagtttcag ttcggcctgc
      541 ttatcgcgaa atccgcgtaa actgttattg ttatgaaatt tccaggcatc tttaaagacc
      601 tgaaagatat agacatcctc aatccaggag tcacattttt taagcgggcg ctttgctatt
      661 atcacccgct catccggatt tagcatcgcc gtaaattgta cggtgacgtc atttgtcgtg
      721 ttcaggtcac attgaacatc tggtacatca ctgatatagg ggtcaaatcc aaaatcagca
      781 ccacaggcac aaagacggct aatggtggcg gtatcgaggg aaaatcccgg ccataccttg
      841 ctggaggtta agccaatatt taatgtgaca tcatatcgaa gcaatccctc aagaccgtca
      901 gcaccgacga acttttcccc ttcatccaag gcctggttaa taagttgctg aacaatgcct
      961 tcatcccagt atgccgattt gcagttggca atcgtaatat ccacgcctgt gaagggatca
     1021 gtaccggcaa ggcttgcaaa taatgtatta actaattcca tttttattat ccagctttaa
     1081 ggtagcgtct tatacatcgg gacataacct acatcttcca ccaaactctg tccctgcggc
     1141 gttaaaaacc actccaacaa tttttgtgtc tctgacgtcg tattttcccg cgttaccata
     1201 aaggcatcga cgatataggg atatttgccg ttgcgaatgt tttcggcagt cggtgcaata
     1261 ccgttaatcg ctagcagttt tatattttta tcggcgttca tttgtgttgc gtaataacgg
//
LOCUS       DAZRUF010000429         1301 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZRUF010000429 DAZRUF010000000
VERSION     DAZRUF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.432"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..317
                     /locus_tag="ACHXOL_004334"
     CDS             <1..317
                     /locus_tag="ACHXOL_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR6811127.1"
                     /translation="KEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMN
                     EDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSG
                     LS"
     gene            328..765
                     /locus_tag="ACHXOL_004335"
                     /pseudo
     CDS             328..765
                     /locus_tag="ACHXOL_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            765..1142
                     /locus_tag="ACHXOL_004336"
     CDS             765..1142
                     /locus_tag="ACHXOL_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811128.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          426 a          200 c          343 g          332 t
ORIGIN      
        1 agaaagagcc agaaatgacg gtcagatatt atatcagttc tgctgattta accgcagaaa
       61 agttcgccac agcaatccga aaccactggc acgtggagaa taagctgcac tggcgtctgg
      121 acgtggtaat gaatgaagac gactgcaaaa taagaagagg aaacgccgca gaattatttt
      181 cagggatacg gcacatcgct attaatattt taacgaatga taaggtattc aaggcagggt
      241 taagacgtaa gatgcgaaaa gcagccatgg atagaaacta tctcgcgtca gtccttgcgg
      301 ggagcgggct ttcgtaatct ttccctaggg agtcagccga atgttgggaa ggatttgacg
      361 gatgaagaga agaactgctt ggccggcggc agtacgggaa gcccgggagg atggggacca
      421 gaagatgaga atagcgcaag ggatcgtatg cagcatgaac ggtataaaga tgaattgcga
      481 cagaaaatgg gaaaacccga cgttacagat tcaaatttga aaaatattat agacgatctt
      541 tatcggccaa atgcaaaagt aggaagtgga agcacagcag atgctgttcg ttatgaattg
      601 gccacaggtg aaaaagttgg tggtagagga catgtattga aagcacagga ttatacaagg
      661 gctttacagg attggttgga taagaatcca acagcatctc caggggatcg agcagcagca
      721 gaaaacgttc ttcaggattt acagaatgct ttgggaggca agtaatgaac caatatattg
      781 ctagttatat agaaaaaatg gcatcatttt ccactgtttt gtctgagaca gtctcattag
      841 ctaatgaata ctggcatcca gaaacgccac cggtggttat gttgttctct caggttggaa
      901 aaacgattgt aaaaaatttt tatgaaatgg gggatattga aagagaaaaa atatttaaac
      961 atgtcgaaga tggtatggtc tcgcttgatg atgaattggc cacagttgta gctacagggc
     1021 ttatagaggc aatagtcact gcgaccgata gcaattcttt actttggcaa gatttaagta
     1081 aatttctggg tgacaaatca aagtctcatg cattagtgtg gaaaaatttt ggtcaggagt
     1141 gatattattc aagaagtatt aagctcgcta ttgagatctt aatgcttctt gagattaaat
     1201 taaagttagg acgaagtaat tgcaggtgga aataaatcca tagtgacgtg tggcaatggc
     1261 tattagatgt tatggtgtga tgggctttgg tgatttaaga g
//
LOCUS       DAZRUF010000430         1299 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZRUF010000430 DAZRUF010000000
VERSION     DAZRUF010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.433"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..1117
                     /locus_tag="ACHXOL_004337"
     CDS             <1..1117
                     /locus_tag="ACHXOL_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR6811129.1"
                     /translation="TWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTVEQMES
                     QMEAEYIPERRLHLYHCDHRGLPQALISPEGETAWRGEYDEWGNQLNEENPHHLYQPY
                     RLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGL
                     ERVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGA
                     GDPYTGEVVGCETNGSGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTM
                     GCTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
     gene            1095..>1299
                     /locus_tag="ACHXOL_004338"
     CDS             1095..>1299
                     /locus_tag="ACHXOL_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811130.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIK"
BASE COUNT          356 a          263 c          400 g          280 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacact gatccactgg ggttagaacg
      661 agttataagt gtatatgggc ctcccgctcc agaccgggca ggtgctgaaa ctcctcttgt
      721 tcttactgat atgactggag gagtgactat ttattatgat cctgaaacag gtgattcaat
      781 gacttttgat agtagcaata gaattgatcg tcgttctcaa cgtggtgctg gtgatccata
      841 tacaggtgaa gtcgttggtt gtgagacaaa tggatcagga atatcggcag catatggcac
      901 aacgaagatc tatactacag atactagagc aagatggcta catggtgggg gatcatcgtt
      961 gcgcgatcct tatgctcctc gacaaggctg gaaaccaact atgggatgta cccgtgcaca
     1021 gaatgaagat gtagatgagt tgtgtaagaa agtgacaagt tggatgtatt cgcatcctgg
     1081 agagagaatt agatatgaac gttttaagac acgttaatgc gattaacatg ggagtgtgca
     1141 ttctaatttt ggttattgga tgtgcaaatg ctaaagttct taaacgatgt atattacccg
     1201 ctcctgatgg tggcgtagaa aaactatcaa aacccaactt acatggagta attaaaaaag
     1261 ttgatttgaa cacaaatgaa gcggaaatta aaataaaag
//
LOCUS       DAZRUF010000431         1275 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZRUF010000431 DAZRUF010000000
VERSION     DAZRUF010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.434"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>1275)
                     /locus_tag="ACHXOL_004339"
     CDS             complement(<1..>1275)
                     /locus_tag="ACHXOL_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR6811131.1"
                     /translation="AYSSYRTRTPAPVGVFGPGWKAPFDIRLQIRDEGLILNDSGGRS
                     IHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALWQVLPEDVRLSPHVYLATNS
                     LQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTG
                     VMDGAGRRFHLVLTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGAD
                     NGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHAG
                     RMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTE
                     GEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVILPD
                     GRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKG"
BASE COUNT          200 a          480 c          358 g          237 t
ORIGIN      
        1 cccttctcat catactcccg gctgctgcga agcccgtccg ggtacgtcac cgacgtcacc
       61 tgccgctggc tgttatagcc ataccgtacc gtcctgccgt ccggcagtat cactgaagtc
      121 agctttccag acgccatatg cagtcggtac tccgtccgcc gtcccgccgc atccgtctgc
      181 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc ggcatgctcc
      241 ttcttcacca cccgcttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
      301 aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc cagcccctcc
      361 gggttgacct gctccgtcac ccgaccggta tcatcatacc ggtagcagct ctccggcctc
      421 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagt aaacccgcgc
      481 acctgcatcc cgctgcggtc atacaccgcc cgcagttcac caccggccgt gtacgtgtag
      541 cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccatacc
      601 gcctccagcc ggataccgtt atcggcaccg tactctgtac cggcgggcag cgtgtcgggg
      661 aaggaaggag ctgagagagg gcagggactg tcaggggaag ataacgaagc ggtgtgcggt
      721 ttacgggcct cttccgcccg ctgcgcctgt gtggtcagca ccaggtgaaa acggcggccc
      781 gcgccgtcca tcacccccgt caccgcgccc gccacgtcac cctccgctgc ccggtgaaag
      841 gccagcgtcc gcccgaagcc gtccaccacg cccgtcagca cccggtatgc gggcggctcc
      901 ggcggcagca cctcgtccgc ccccggcacc cgctccggcc agctgagtat ccaccacggc
      961 ccctgaaggc tgtttgtcgc caggtacaca tgcggactca ggcgaacatc ttccggcagc
     1021 acctgccaga gcgcgcttag cggctgcgaa ctgtgctgcg ccgctactcc gccccgcgcc
     1081 agccagagtg actcgctgcg gctgtagctt atctcgccgg gaaacagcgg ctcaaagtga
     1141 atgctcctgc cgccgctgtc gttgagtatc aggccttcat cgcgtatctg taagcggata
     1201 tcgaagggcg ctttccagcc gggaccaaac acccccaccg gcgcgggtgt tctggtccgg
     1261 tagctgctgt aggcg
//
LOCUS       DAZRUF010000432         1275 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZRUF010000432 DAZRUF010000000
VERSION     DAZRUF010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.435"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>1275)
                     /locus_tag="ACHXOL_004340"
     CDS             complement(<1..>1275)
                     /locus_tag="ACHXOL_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811132.1"
                     /translation="RNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTD
                     CSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDL
                     TAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDP
                     VDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQW
                     QYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAYS
                     EQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYE
                     LATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHT
                     TAANLDIDIPYATDPAGNRLPDPELHPDSTLTA"
BASE COUNT          187 a          435 c          366 g          287 t
ORIGIN      
        1 cgccgtgagg gtgctgtccg ggtgcagttc cgggtccggc agccgatttc ctgccgggtc
       61 tgttgcatac gggatatcga tatccaggtt cgctgccgtg gtgtggacgc ccgtcagcct
      121 ccccgtgtcg ctgtaacggt actcccggct ctcctgcggg ccactgatgc ggattagctg
      181 tccgttgtcg ttccagtcgt agtcacggtc aagctgcgga aggttcaggt gcctgctccg
      241 gagctggccg ctggtattcc aggcggtggc aagttcatat gcctccccgc cgaagctgcg
      301 ggccgtctca cggtgcagcc ggtcccgcgt gtactcgacc agcggtgttc cgcccagctt
      361 catccccgca agataaccgc tgccatacgt cagccactcc accggcggca ggccgtccgg
      421 ctcctgacgg gtcgccagcc cctgttcgct gtacgcgtgc cccgtctcat gctcccacag
      481 catctccccc gtctccgggg tctccaccgt ctgccgttcg cccgtcagac ggcctttatc
      541 gtcatagccg tagtggaccg ccacacggtg gccttcactc aggtggctga tttctgtcag
      601 ccagccgtgg tcgtcatact gccactgctc cgccggttca ccgttcaccg tgcgccgggt
      661 gatacggtcc gacgcatcgt aatgccagag ggtgataagc ccctcatctt cactctgcgt
      721 gagttttccg gtcaggtcat actggtaacg ttgcgtccgg ccgtcaaagc cgcgctgctc
      781 cgtcagcctg tcgaccgggt cataccggaa cgtggactgg ctgccgttct cgttggtcag
      841 cacagtgatg cgcccggcgg cgtcataccc catgcttcgc gtcagaccgc cctgcgtggt
      901 gctgacggcc tttccccacg catcatactg tatctcactg cggctgccgt ccggggcaac
      961 gatagcggtg aggtctcctg cggcgctgta ctcataacgg gtttcacgcc cctgcgcatc
     1021 cctctgactg accagttgac cgcgcgggtt ataactgctg taagtgctga tgccttcttc
     1081 ccggtgaacg gcgatttgtt gaccgtaccg gtcatactca taccgcgtcg tgtaccccga
     1141 gcagtccgta aacgcctgaa gctgaccgta ccggtttcgc gttattctcc gcctcacccc
     1201 ggttccgtct tccaccgcac agggcaggcc actgcgggaa gaatcataaa accagcgcgt
     1261 aatgttaccg ttgcg
//
LOCUS       DAZRUF010000433         1254 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZRUF010000433 DAZRUF010000000
VERSION     DAZRUF010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.436"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            98..1180
                     /locus_tag="ACHXOL_004341"
     CDS             98..1180
                     /locus_tag="ACHXOL_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR6811133.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          361 a          265 c          275 g          353 t
ORIGIN      
        1 accaaaactt acatcttgaa ataatcacat tgattagatg aatatttatc gcgcagtgac
       61 atcatttttt aataatagtt caaaaaaagg gctcacgatg aaaaaattaa cagtggcaat
      121 ttctgctgta gctgcatcag tactgatggc gatgtctgct caggcagctg aaatttataa
      181 taaagacagt aacaagctgg atctgtacgg gaaagttaat gctaagcact acttctcctc
      241 taatgatgca gatgatggtg atactactta tgcccgtctt ggcttcaaag gtgaaaccca
      301 aatcaacgat caactgactg gtttcggtca gtgggaatat gaattcaaag gcaaccgcgc
      361 tgaatctcaa ggttcctcca aagataaaac ccgtcttgcc ttcgctggcc tgaaattcgg
      421 tgactacggc tccatcgatt acggccgtaa ctacggtgta gcatacgaca tcggtgcgtg
      481 gactgacgtc ctgccagaat tcggtggtga cacttggact caaacagatg tgttcatgac
      541 tggtcgcacc actggtgttg caacttatcg taacaacgac ttctttggtc tggttgatgg
      601 tctgaacttt gctgctcagt atcagggtaa aaatgaccgc actgacgtaa ctgaagctaa
      661 tggtgatggt ttcggtttct ccactactta tgagtatgaa ggattcggtg taggtgcaac
      721 ctatgctaaa tctgaccgca ctaataatca ggttatctac ggtaacaaca gcctgaatgc
      781 atctggtcaa aatgctgaag tatgggcagc tggtctgaaa tatgatgcga acaacatcta
      841 tctggctacc acctattctg aaacccagaa catgactgtt tttggtaata accatattgc
      901 caacaaagca caaaacttcg aagtagttgc acaatatcag ttcgacttcg gtctgcgtcc
      961 gtccgttgct tacctgcaat ctaaaggaaa agacttgggt gcgtggggtg atcaggacct
     1021 ggttgaatat attgatgtag gtgcaaccta ttacttcaac aaaaatatgt ccacttttgt
     1081 tgattacaaa atcaacctga ttgataagag cgatttcacg aaagcatctg gcgttgctac
     1141 cgatgatatc gttgctgtag gtatggttta ccagttctaa tttgattact aaaagatatg
     1201 ttgcgggagg ctttgcctcc ccaacatata agtggctccc tcaagccact tcct
//
LOCUS       DAZRUF010000434         1251 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZRUF010000434 DAZRUF010000000
VERSION     DAZRUF010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.437"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            127..810
                     /gene="tssD"
                     /locus_tag="ACHXOL_004342"
                     /pseudo
     CDS             127..810
                     /gene="tssD"
                     /locus_tag="ACHXOL_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            790..>1251
                     /locus_tag="ACHXOL_004343"
     CDS             790..>1251
                     /locus_tag="ACHXOL_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811134.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT          392 a          243 c          267 g          349 t
ORIGIN      
        1 aagatattaa agtgaaacat tttgtttttc acattttagt tcatcacttt taggcgtaaa
       61 taaaggtaaa aaaaacaaca atactatttc tctttcacaa aagaattaac aaaaggtttt
      121 tcaaggatga gtgatattat ttacctgacc ataaccggag aacaacaagg atgtatttcc
      181 tctcgttgtg gaacgagcgc atcaataggt aatcgctggc agattggcca tgatgatgaa
      241 atcttcgcgt tctcgctctc taatagtatt acgaatacag gcaaagggtc ataattgcat
      301 ggccttagtt tctgtaaatt gattgacaaa agctccccgt tacttattaa cgctattaac
      361 aataacgagc agttgttcat ggagtttgac ttctatcgga tcaataggtt tggtcgatgg
      421 gagaaatatt acaacattca actccgcggc gcgctcttat cagcaatcaa tcacctgttt
      481 actgagaaca atctggatac agaagccatc actgtcagtt attaatatat actctccagg
      541 catctgattg ctaatactga atttagttat tgggcatttc ccgataacta caaccgtctg
      601 tttataccac ggccaaaaac taaagctggt aatggtttca aaacacttaa tagtaaagcg
      661 gttggcagac tgcttgctgc tgtcggcata tacaatggga atatcgaagg attcagggaa
      721 acagcagaca agttgggggg agatgcacct gctggttatg atcaatttat ggataacaaa
      781 gggttactca tcgctggtgc ttcaatagtt gctggagcag ttctggggag aatgagactc
      841 cctgatttgg aatcacttga gcatttcgga gccagaggag cagtatcagg aagaccattt
      901 aatcccgagc ttgccggtgg tccaatagaa aacctgacga cagatggcgt aacaatcaac
      961 agggaaggta tcgctattgt tgaaaaacat atagccagat ttggtcatga tcctgtaaat
     1021 gaagtgatga tcaatagact gaaagacatt gagaaaggaa aaatcccccc agaacaagta
     1081 gatcttaact tctatactca tgagtgtcgg gaatatcagc gttactgtaa tcttggttgg
     1141 gaaacaggac aacctgatgg tgacgcaggc tatgcactct ggaatcatac tggatttgcc
     1201 cctatatttc cagacatctg ttatcactta acccattaca agcccgctgc c
//
LOCUS       DAZRUF010000435         1243 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZRUF010000435 DAZRUF010000000
VERSION     DAZRUF010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.438"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            273..>1243
                     /gene="cadB"
                     /locus_tag="ACHXOL_004344"
     CDS             273..>1243
                     /gene="cadB"
                     /locus_tag="ACHXOL_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR6811135.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT          283 a          264 c          295 g          401 t
ORIGIN      
        1 cataattttt attacataaa tttaaccaga gaatgtcatg caatccattg taaacattga
       61 atgtttatct tttcatgata tcaacttgcg atcctgatgt gttaataaaa aacctcaagt
      121 tctcacttac agaaactttt gtgttatttc acctaatctt taggattaat ccttttttcg
      181 tgagtaatct tatcgccagt ttggtctggt caggaaatag ttatacatca tgacccggac
      241 tccaaattca aaaatgaaat taggagaaga gcatgagttc tgccaagaag atcgggctat
      301 ttgcctgtac cggtgttgtt gccggtaata tgatggggag cggtattgca ttattacctg
      361 cgaacctagc aagtatcggt ggtattgcta tctggggttg gattatctct attattggtg
      421 caatgtcgct ggcgtatgta tatgcccgac tggcaacaaa aaacccgcaa caaggtggcc
      481 caattgctta tgccggagaa atttctcctg catttggttt tcagacaggt gttctttatt
      541 accatgctaa ctggattggt aacctagcga ttggtattac cgctgtatct tatctttcca
      601 ccttcttccc agtattaaat gatcctgttc cggcgggtat cgcctgtatt gctatcgtct
      661 gggtatttac ctttgtaaat atgctcggcg gtacctgggt aagccgttta accactattg
      721 gtctggtgct ggttcttatt cctgtggtga tgactgctat tgttggctgg cattggtttg
      781 atgcggcaac ttatgcagct aactggaata ctgcggatac cactgatggt catgcgatca
      841 ttaaaagtat tctgctctac ctgtgggcct tcgtgggtgt tgagtccgca gcagtaagta
      901 ctggtatggt taaaaacccg aaacgtaccg ttccgctggc aaccatgctg ggtactggtt
      961 tagcaggtat tgtttacatc gctgcgactc aggtgctttc cggtatgtat ccgtcttctg
     1021 taatggcggc ttccggtgct ccgtttgcaa tcagtgcttc aactatcctc ggtaactggg
     1081 ctgcgccgct ggtttctgca ttcaccgcct ttgcgtgcct gacttctctg ggctcctgga
     1141 tgatgttggt aggccaggca ggtgtacgtg ccgctaacga cggtaacttc ccgaaagttt
     1201 atggtgaagt cgacagcaac ggtattccga aaaaaggtct gct
//
LOCUS       DAZRUF010000436         1236 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZRUF010000436 DAZRUF010000000
VERSION     DAZRUF010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.439"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHXOL_004345"
     CDS             complement(<1..815)
                     /locus_tag="ACHXOL_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR6811136.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHXOL_004346"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHXOL_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHXOL_004347"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHXOL_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          342 a          212 c          330 g          352 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttt
//
LOCUS       DAZRUF010000437         1215 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZRUF010000437 DAZRUF010000000
VERSION     DAZRUF010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.440"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..1060
                     /locus_tag="ACHXOL_004348"
     CDS             <1..1060
                     /locus_tag="ACHXOL_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR6811137.1"
                     /translation="IKQNAEEQIRAEQKDVDFETKEFTVELLVNKYHNGLEDDTNELF
                     VPDYQRDFVWSEKRQSKLIESLILGFPIPYIFIADVLSEDPDLDGRIEIVDGSQRVRT
                     IHAFIQNELVLQDLKSLDVLNGFKFQDLPLSRQRRFMRIPVRVIELSSKCNEETRRDL
                     FERINSGSDILKDMEVRKGSELGSTNLYTQVIKPCSTIPKFKELAPLSQTKEKRDERL
                     EFTLRFFAYVDKYKNFDHSVRDFLNDYMSENRDIDETKQNSMKQEFANVLSFVENYFP
                     AGFKKTITAKSTPRVRFESLAVGAALALREVPDLVPKNLDWLSSEEFKRLTTSDGANS
                     RVKVIERIEYVRDKLLED"
BASE COUNT          407 a          203 c          238 g          367 t
ORIGIN      
        1 gataaagcaa aacgctgaag agcaaattcg tgctgagcaa aaggatgtgg attttgagac
       61 caaagaattt accgttgagt tattagttaa taagtatcat aacggattag aggatgatac
      121 taacgaactc tttgttcctg attatcaaag ggattttgtt tggagtgaaa aaagacaatc
      181 aaaattgata gagtctttga ttttgggttt tcctatacca tatatattta tagcagatgt
      241 tctctccgaa gatcccgatt tagatgggcg aattgagatt gttgatggct cacaacgagt
      301 taggactata cacgctttta tccaaaatga attagtttta caagacctga aatctttaga
      361 cgtccttaat ggtttcaaat tccaagattt accattatca cgtcaacgtc gtttcatgag
      421 aattcctgtc cgcgttatag aattgagttc taaatgcaat gaagaaaccc gtagagatct
      481 ttttgagcgt attaactctg gcagcgatat attgaaagat atggaagtcc gtaaaggatc
      541 agaacttggt tcaacaaatt tatatacaca ggttattaag ccatgctcaa caatacctaa
      601 atttaaagaa ttagcacctt tatctcagac taaagaaaaa cgagatgaac ggttagaatt
      661 tacattaaga ttctttgcct acgtagataa gtacaagaac tttgatcatt ccgtacggga
      721 ttttctaaat gactatatgt cagaaaatcg agatatagac gaaactaaac aaaactcaat
      781 gaaacaagag tttgctaatg ttctatcttt tgttgaaaac tatttcccag caggattcaa
      841 gaaaactatt actgcaaaat ctacacctcg agttagattc gaatctctcg ctgttggcgc
      901 agcacttgca ttacgtgaag tccctgacct tgttcccaag aacttagatt ggctaagctc
      961 agaagaattc aaacgattga ccacttccga cggagctaat agtcgcgtga aggtaattga
     1021 acgaattgaa tatgttcggg ataaattatt agaggattaa catggatcgt tttcgtgagg
     1081 attttgacga aaggtcagga gagatcttgg cctatttgga tcttttgaaa tttattgagt
     1141 atgccggtgc agaactgata tcagctgatg ataaagaaca taagttctcc attactgctc
     1201 aatcacgaaa aactc
//
LOCUS       DAZRUF010000438         1215 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZRUF010000438 DAZRUF010000000
VERSION     DAZRUF010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.441"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            490..>1215
                     /locus_tag="ACHXOL_004349"
     CDS             490..>1215
                     /locus_tag="ACHXOL_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR6811138.1"
                     /translation="MNRKQFIQSHGATCSNWTWSWSFVNHDKKMVIFGAWDVQKEQER
                     SVILRERWKTKVVEGKERKNLGYIQAIEHIQLIAEGYELYTFDMEQGRNDENRDVAVI
                     KRFTPKLEKRYLRKEGTVWYADFLPNPFPDEITSPENYAEGAKKQVTVNSYERDPKAR
                     QACIDHHGTSCGCCEFNFEKVYGEHAKGFIHVHHIKPLHTVGEDYEVNPITDMVPLCP
                     NCHAMVHRGNEVLSVESLKALLFK"
BASE COUNT          385 a          235 c          265 g          330 t
ORIGIN      
        1 tcgagtactt aaagggcttg cgttaattgt aacttattac ggcatattac ggcatggatg
       61 tatgccgtaa tacggcaaaa aaagcccctg aaacccgcta tttaccatca atacggcaac
      121 taagtataga gatacgaaat ccattccatt tcattccatg tatttctttc tctatcagta
      181 ttccaagaaa ctgtgacttg cgtcacgaat actactcgtc gtgaactcct cgtagtattg
      241 gttagtaacg ttgcgttttt ctcgccagta ctcgaaaacc cgcaaggcgt tactactcgc
      301 tttgctcgat agatgttagt acagcaaatg aagtacctaa agtgaattgg taagtacgac
      361 atgccccgcc agttccacaa ctctcgctct ggtcatttcc actactatga tgctattgga
      421 tagctacata aagtactgga tcaagtaggc attttttgtg tacaaagagg gaattaagag
      481 agggatacca tgaacagaaa acaattcatc cagtcgcatg gtgctacttg tagtaactgg
      541 acttggagtt ggtcatttgt gaaccacgac aagaaaatgg ttatctttgg tgcatgggat
      601 gtacaaaaag agcaagaaag atcagtcatt ctgagggagc ggtggaaaac caaagttgtg
      661 gagggaaagg aaagaaaaaa tcttggatat attcaagcta ttgaacacat tcaattgata
      721 gctgaaggtt atgagcttta cacgtttgat atggagcagg gacgaaacga tgagaataga
      781 gatgtagctg ttatcaaacg gttcactcca aaacttgaaa aacgttacct tcggaaagaa
      841 ggaacagttt ggtatgcaga ctttttacct aatccatttc cagatgaaat tacctcacct
      901 gaaaactacg ctgagggtgc aaaaaagcaa gttacagtaa actcctacga gcgtgatcca
      961 aaagcacgac aggcttgtat tgaccatcat ggtacttcct gcgggtgttg tgagtttaac
     1021 tttgagaaag tctatggtga acacgccaag ggctttatac acgttcatca tatcaaaccg
     1081 ctacacacag taggcgagga ttacgaggta aatccaatta ctgatatggt tccactttgt
     1141 ccaaactgtc acgcaatggt acatcgcggt aacgaagtat taagtgtcga aagtttaaaa
     1201 gcactgttgt ttaaa
//
LOCUS       DAZRUF010000439         1206 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZRUF010000439 DAZRUF010000000
VERSION     DAZRUF010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.442"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            175..693
                     /locus_tag="ACHXOL_004350"
     CDS             175..693
                     /locus_tag="ACHXOL_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR6811139.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            903..>1206
                     /locus_tag="ACHXOL_004351"
     CDS             903..>1206
                     /locus_tag="ACHXOL_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR6811140.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          292 a          306 c          312 g          296 t
ORIGIN      
        1 agatttaatg attttaaaaa tagtttttac gacaaccgta aattctagct cggtttgtat
       61 gtaaaaaata ctgaaaaatg atgcggttcc gtgaagtgag ctgcatctgc ctgtctggga
      121 ttaacggcag gtcattgtaa gtagtaatta accacgttaa caaggagttt acaaatgcca
      181 acaccgtgtt atatctccat cactggtcag acccagggaa atattactgc aggtgcattt
      241 accgctgatt ctgtcggcaa catctatgtg caggggcacg aagacgagat gctggtgcag
      301 gaattcctgc ataacgttac tgtcccgacc actcctcagt ccggtcagcc tgcgggtcag
      361 cgtgctcaca agcctttcat ttttaccgtg gcgctgaaca aagcggttcc gctgatgtac
      421 aacgcactgg cctccggcga aatgctgcca accactgaac ttcactggtg gcgtacctct
      481 gtggagggca aacaggagca ttatttcact acccgcctga ctgattcgac gattgtcgat
      541 atgaaactgc atatgccgca ttgccaggac ccggcgaaac gcgagttcac ccagttgctc
      601 gaggtttcac tggcctaccg caaaattgaa tgggaacacg tcaaatctgg cacctccggt
      661 gccgacgact ggcgcgcacc actggaagca taagttaagc caacagcatc cggctatcag
      721 gccggatgct gtttttgtat tacgccgtag aaagaaaggt tgtcagtaag gggttacgtt
      781 cacgggagat ggcggatgcc ggaaacttcg gcggtagcgt gcctgaacgc aagcgtaaca
      841 ctgtactgtc acagccgctt taacagcgaa ctgtctgttt ttccttaacc ccggaggttg
      901 ctatgtcaac cggattacgt ttcacgctgg aagtggacgg cctgccaccg gatgcttttg
      961 cggtggtctc ctttcatctg aaccagtcac tctcttcgct cttttccctc gagctctctc
     1021 tggtcagcca gcagtttctc tcccttgaat ttccgcaggt actggacaaa atggcctacc
     1081 tgacgatatg gcagggcgat gacgtacagc gccgggtgaa aggtgtggtg acctggtttg
     1141 aactggggga gaacgacaaa aaccagatgc tgtacagcat gaaggtatgc ccaccgctgt
     1201 ggcgca
//
LOCUS       DAZRUF010000440         1201 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZRUF010000440 DAZRUF010000000
VERSION     DAZRUF010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.443"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>1201
                     /locus_tag="ACHXOL_004352"
     CDS             <1..>1201
                     /locus_tag="ACHXOL_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811141.1"
                     /translation="YTQSNAFNFTLEMNRLKVRKPTIGIIAQGFNDGTYSEEVKITVE
                     NKQCGLVEDVSFTGGFGNLFASWTKSTERDYAGAIISIVNGTNTRIFTSYAPEFDSIP
                     NITDGEYKVKMGFFDVFGTDDIQYCPEQTISINSKYQFTEDDAEAINGILDLEDRLTG
                     TINDAVKIANKNTSTVISASEARTNDKITASEKTLSTQITGVNSSLSQKLSTVESTAN
                     GNKASIQTLSQTVTDNNKAQTTAVSQLRSDVNGQIATVNQSMSTKADKSTVDAQYSLS
                     VQANGTVAGMRLVASSGTSNNSAIYFAANKFIVSGTDTATVGGTAPFAIVNGTTYLKT
                     AMIQAASIGTGYIADAAITNLKLASGSVNTLNVVDGCITTAKISNTIQSTNYVANKTG
                     WQINKAGT"
BASE COUNT          389 a          230 c          258 g          324 t
ORIGIN      
        1 tatacgcaaa gtaatgcctt taatttcact cttgaaatga accgtctgaa agtacgtaag
       61 cctaccattg gtattatcgc acagggattt aatgatggta catattcaga ggaagtgaaa
      121 ataaccgtag agaataaaca gtgtggttta gtagaggatg ttagctttac gggtggtttt
      181 ggtaatttgt ttgcttcatg gactaaatct actgaacgtg attatgcagg tgcgattatc
      241 agtattgtta atggtactaa tacacgtata ttcaccagtt atgcacctga atttgattca
      301 ataccaaaca tcactgatgg tgagtataag gttaagatgg gattctttga tgtgtttggt
      361 actgatgata ttcaatattg tccagaacaa acaatcagta tcaactccaa gtaccagttt
      421 accgaagatg atgcagaggc aattaacggg attcttgatt tagaagatcg cctgactggt
      481 accattaatg atgcggtcaa aattgccaat aagaatacca gtactgttat cagtgcatca
      541 gaggcacgta ccaatgataa aatcactgcc agtgagaaaa cgttaagtac tcagatcact
      601 ggggtaaata gttccttatc acagaaactt agcaccgttg aaagtactgc caatggtaac
      661 aaggccagta tccagacact atcccagacg gtaacggata acaacaaagc tcagactaca
      721 gcagtctcac agctacgtag tgatgtgaat ggacagattg ccactgttaa ccagtcaatg
      781 agtactaaag ccgataaatc tactgtagat gctcagtact cactatcagt acaggctaat
      841 ggcacggtag ctggtatgcg tcttgttgcg agtagtggaa cgagtaacaa ctcagcaatc
      901 tatttcgctg cgaacaagtt catagtctcc ggtactgaca ctgctactgt aggtggtact
      961 gcaccatttg ccatagtcaa cggtactaca tacttgaaga cggcaatgat acaggcggca
     1021 agtatcggta ctggttacat cgctgatgcg gcaattacca atctaaaact ggcctccggc
     1081 tcagtgaata cattaaacgt ggttgatggt tgtataacta cagccaaaat ctcaaatact
     1141 attcaaagta ctaattacgt agcaaataaa acaggctggc aaatcaataa agcaggtacg
     1201 a
//
LOCUS       DAZRUF010000441         1193 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZRUF010000441 DAZRUF010000000
VERSION     DAZRUF010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.444"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            196..468
                     /locus_tag="ACHXOL_004353"
                     /pseudo
     CDS             196..468
                     /locus_tag="ACHXOL_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
     gene            complement(434..841)
                     /gene="nudG"
                     /locus_tag="ACHXOL_004354"
     CDS             complement(434..841)
                     /gene="nudG"
                     /locus_tag="ACHXOL_004354"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR6811142.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            928..>1193
                     /locus_tag="ACHXOL_004355"
     CDS             928..>1193
                     /locus_tag="ACHXOL_004355"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR6811143.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
BASE COUNT          289 a          295 c          304 g          305 t
ORIGIN      
        1 ttgtttatgt catgtgcttt tgcagttttc gactatacca tcaagaatgt gacggtaagc
       61 aagaaaaaaa tgacccggga aagcaaatta ataacgagag taatctcata accaaatgcg
      121 ataactcaga cgctaaagtt ttgccacccg gctaccgtta tcagttgcag agacaataac
      181 gttaaggagt tccctgtgag tcgagcattg ttcgccgtag tattagcatt tccattaatt
      241 gcactggcta acccgcatta tcgtccggac gtggaggtaa acgtaccgcc agaagtgttc
      301 agttcaggcg ggcagtcagc gcagccctgt actcagtgct gcgtttatca ggatcaaaat
      361 tattctgaag gggcggtaat taaagccgaa ggaatactgc tgtaatgcca gcgggatgac
      421 aaaaccctta gcactaatcc gctgctctgg cggcgcgtaa agccataaac gcctctaata
      481 gtggaatgtc agcaggggcc agcggatatt gcagcgcctc ttcaggtgag caccagacca
      541 gcgcctgatg ttcatgtgcc tgtaacgtcc cgtggaagtc gggtacgtgc caggcatgaa
      601 gatggataat ccggcccgaa acttctcgct gatggctggc aacatactca cccacagttg
      661 cttcgatgcc caattcttcg tttaactcac gcaccagtgc ctgctgctgg ctttcatcca
      721 gctcgacttt accatcggca aactcccata atcccgcctg atcgctctgg gcggggcgtt
      781 gcgcgagtaa aattttgcca tcacgttcaa tgatggcggc aacaacttca atcattttca
      841 taaattacac acgggcagat aagcgaaaaa tgccataata tccgcactgt tgctcaatca
      901 gaaaggcttt atatttaagg agtgcctgtg ctagaccgcc atcttcatcc ccggattaaa
      961 ccgttgttgc atcagtgcgt gcgggttctt gataaaccgg gcattacgcc agatggttta
     1021 acattagtgg gatttgccat cggcgtgctg gctctgccgt ttctggcgct gggctggtat
     1081 ctggcggcgc tagtcgtcat tttgttgaac aggctgcttg atggtctgga cggtgcgctg
     1141 gcgcggcgca gagagcttac cgacgcgggc ggctttcttg atatttctct tga
//
LOCUS       DAZRUF010000442         1191 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZRUF010000442 DAZRUF010000000
VERSION     DAZRUF010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.445"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..100
                     /gene="mgtT"
                     /locus_tag="ACHXOL_004356"
     CDS             <1..100
                     /gene="mgtT"
                     /locus_tag="ACHXOL_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR6811144.1"
                     /translation="GDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHXOL_004357"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHXOL_004357"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR6811145.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT          337 a          291 c          236 g          327 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga a
//
LOCUS       DAZRUF010000443         1187 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZRUF010000443 DAZRUF010000000
VERSION     DAZRUF010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.446"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..755)
                     /locus_tag="ACHXOL_004358"
     CDS             complement(<1..755)
                     /locus_tag="ACHXOL_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR6811146.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTIGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFSEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELVEHLKKA"
     gene            1071..>1187
                     /locus_tag="ACHXOL_004359"
     CDS             1071..>1187
                     /locus_tag="ACHXOL_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811147.1"
                     /translation="MYQRCFDNASETLFVVGKTPRLSRFAFSDDPKWESGHHV"
BASE COUNT          290 a          286 c          289 g          322 t
ORIGIN      
        1 gctttcttca gatgttccac cagctcggca atggcctccg gcgaatcgtt atcgagaatg
       61 atgtgcttac gttctgtttg cggcggtacg cgaatgccgg tcagttcagc aagtggcgcg
      121 ctctggctcc agccaatatc acttgcctgc cactgattta ccggtttttt acccgcgccg
      181 agaatggctt tcatcgaagg aatacgtggc atgttaatat cggaggtgac gcagagcacg
      241 gctggaacag agagctcaat aacttcaaca tcatcttcaa gcgtgcgttc aatcaccagt
      301 gtattgccct gacgctgaat agcactcact gcattaatca ccggaagttg cagaatttct
      361 ccgaccagca agccaacctg ctgggcataa aggtcgccgg aaccttcact aaagatcagt
      421 aaatcgaagc cgatcttttc aactgctgcc gccagcgcct ttgcggtatc gagaggcagt
      481 gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg ggatagcacg
      541 tctttgcgca ctttcgagtt ctgcaacaat gagccaccaa tggtcagcgc ggctatctca
      601 tcgtcatctg ttgcaagctg gcttgcagct tcaatggcat tgagatcgaa ctggctgatt
      661 ttggcgtcgg cattgtcgaa attcagggtg tattctggag tgacaacaat gtcctgttct
      721 tcaggcacca gcttaaagca ggttattatt ttcatgattt ctcctgtgag tcgcatacgg
      781 aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt
      841 tttcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg cgatcgctaa
      901 aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct tgcattcagt
      961 gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata cacgccgtat
     1021 tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc atgtatcaac
     1081 gctgttttga taacgccagt gaaacgctgt ttgtcgtcgg taaaacgcca cggctttcac
     1141 gttttgcatt tagcgatgat ccaaaatggg agtccggaca tcacgtt
//
LOCUS       DAZRUF010000444         1182 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZRUF010000444 DAZRUF010000000
VERSION     DAZRUF010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.447"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..761)
                     /locus_tag="ACHXOL_004360"
     CDS             complement(<1..761)
                     /locus_tag="ACHXOL_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR6811148.1"
                     /translation="MLNVAIENQNGWNYSAPAPYKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHDNVLRAIANTECSPEFNALNFEDVTYT
                     DAKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPTDKI
                     IPGDGRTLVVHFDERGNIRFTETVPDGARVCTMET"
BASE COUNT          287 a          294 c          302 g          299 t
ORIGIN      
        1 aaagtctcca tggtacagac ccgtgcgcca tcaggaacgg tttcggtgaa tctgatgttg
       61 ccgcgttcgt cgaagtgaac aaccagggtg cggccatcgc ctggaattat tttgtcagtg
      121 ggtggggtgt tattctggcg cagttcggcc tccatgcgat cgaactcagc aatgtaggct
      181 tctttaaatg cagcggcttt tttgccagtg aatcccatca ccaggaaaac gaagccgttt
      241 ttggtgattt ggtacatcgg gcgtttttcg cctttggcgt cagtgtaggt gacgtcctca
      301 aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg taacacattg
      361 tcgtgacgct tgccaaagaa ctctgcgatc gcaatagacg tagtgacagc gcgaccattt
      421 tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc atcctcgagt
      481 gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt gagacgtaca
      541 ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc tgcacatccc
      601 accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc gcggctatgc
      661 gctcgagtaa cgttcgggtt tccacgcccg gcacccgttt tataaggtgc aggtgcacta
      721 taattccacc cgttctggtt ttcaatagct acattcaaca ttttctcttt cctttcatca
      781 ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa ccagttaaca
      841 attaacgccg gatacaggga aacccataac cacccgatac gggggaacta atcgcttttt
      901 aactgtgccg cgtgcgctga gccgcattca ccgcatcaca aaattcactt taaaaagggc
      961 ggacatcagc cagcaatgaa actgatgccg ccaaaggtac caatcaacat ggagtgttgt
     1021 ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc aacggggaag
     1081 gaataactcc gccatactta ccgccgcgtc atttcgcgtt gtgtgcctgc ttttaaccac
     1141 gtcaggcgag gtggtatcct tcttatcccg aataaccaag aa
//
LOCUS       DAZRUF010000445         1177 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZRUF010000445 DAZRUF010000000
VERSION     DAZRUF010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.448"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(101..>1177)
                     /gene="ompC"
                     /locus_tag="ACHXOL_004361"
     CDS             complement(101..>1177)
                     /gene="ompC"
                     /locus_tag="ACHXOL_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR6811149.1"
                     /translation="VVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDG
                     DKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISEFGS
                     LDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDFFGLIEGLN
                     FAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQER
                     AAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVA
                     QYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLID
                     ESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT          327 a          270 c          256 g          324 t
ORIGIN      
        1 tgaccagaaa tatcaaaaat tgctcatttt tacttaagga gtcatacagc agcacttaaa
       61 tcaaaaacca gcccagagaa gggctggtca ggagaggacg ttagaactga taggtaatgc
      121 ccgtagcgac gatgttatcg gtccgaatat ctacggcacg ggtaaagtcg ctttcatcaa
      181 tcaggttgat tttataatca acataggtcg acatattttt gttgaagaaa tacgtcgcac
      241 caacgtcgat atactcaatc atgtcctggt cgccgtagcg gcccagatct ctgcctttcg
      301 atttcaggta agcgagtgat gggcgcaagc cgaagtcgaa ttgatattgc gccaccactt
      361 caaaactttg tgctttgtta gagatataac catccccata ggtggtcatg tttaatgttt
      421 cagcgtaatt agctgcaaag tagatgttgt tggcatcata ctttatacct gtggcccata
      481 gttctgcatt cttaccggag gcattgagag gattagcagc ggctctttcc tgcgcatttg
      541 tccgatcgga tttggtatat accgcaccga taccaaagcc atcataaaca taagttgagg
      601 agataccgaa accgtcaccg ttggcacgcg tgtagtcagc accataaaga tgactgttgt
      661 cagtacgttc atttttgcct tgatattgcg cggcaaagtt cagcccttca attaagccaa
      721 agaaatcgta gttgcgatag gttgctagac cggtggtacg gtatgtcatg aagacatctt
      781 tctgactcca ggtatcgcca ccaaattctg gcaacatatc ggtccatgaa ccaatgtcgt
      841 agccgacacc gtagttacga ccatagtcca gagagccaaa ttcactaatt tttaaaccag
      901 cataggccag acgggtcttg ttgccgcgcg agccgttggc ttcatcgtta tagcctttaa
      961 aatcatattc ccagtgacca aaaccaatca tttgatcatt gatttgcgtt tctcctttaa
     1021 agccgagacg ggcataagtt ttatcaccat cgtcacgctt atcatcagta aaataacgta
     1081 gagcggtaac cttgccataa aggtcgagtt tattaccatc cttgttatag acttcggcag
     1141 cgtgtgctac gttcgcagct aacaaaccag ttacaac
//
LOCUS       DAZRUF010000446         1173 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZRUF010000446 DAZRUF010000000
VERSION     DAZRUF010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.449"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>1173
                     /gene="fimD"
                     /locus_tag="ACHXOL_004362"
     CDS             <1..>1173
                     /gene="fimD"
                     /locus_tag="ACHXOL_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR6811150.1"
                     /translation="SHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHIN
                     TWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIA
                     RGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYS
                     SVPLLQREGHTRYSITAGEYRSGNAQQEKTRFFQSTLLHGLPAGWTIYGGTQLADRYR
                     AFNFGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGY
                     RYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQL
                     GRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQML"
BASE COUNT          332 a          261 c          283 g          297 t
ORIGIN      
        1 agccattatg catatttaaa cctacagagt gggctaaata ttggtgcgtg gcgtttacgc
       61 gacaatacca cctggagtta taacagtagc gacagttcat caggtagcaa aaataaatgg
      121 cagcatatca atacctggct tgagcgagac ataattccgt tacgttcccg gctgacgctg
      181 ggtgatggtt atacccaggg cgatattttc gatggtatta actttcgcgg cgcacaattg
      241 gcctcagatg acaatatgtt acccgatagc caaagaggat ttgccccggt gatccacggt
      301 attgctcgtg gtactgcaca ggtcactatt aaacaaaatg ggtatgacat ttataatagt
      361 acggtgccgc cggggccttt taccatcaac gatatctatg ccgcaggtaa tagtggtgac
      421 ttgcaggtaa cgattaaaga ggctgacggc agcacgcaga tctttaccgt accctattcg
      481 tcagtcccgc ttttgcaacg tgaagggcat actcgttatt ccattacggc aggagaatac
      541 cgtagtggaa atgcgcagca ggaaaaaacc cgctttttcc agagtacatt actccacggc
      601 cttccggctg gctggacaat atatggtgga acgcaactgg cggatcgtta tcgtgcgttt
      661 aatttcggta tcgggaaaaa catgggggca ctgggcgctc tgtctgtgga tatgacgcag
      721 gctaattcca cacttcccga tgacagtcag catgacggac aatcggtgcg ttttctctat
      781 aacaaatcgc tcaatgagtc aggcacgaat attcagttag tgggttaccg ttattcgacc
      841 agtggatatt ttaatttcgc tgatacaaca tacagtcgaa tgaatggcta caacatcgaa
      901 acacaggatg gtgttattca ggttaagccg aaattcaccg actattacaa tctcgcttat
      961 aacaaacgcg ggaaattaca actcaccgtt actcagcaac tcgggcgcac atcaacactg
     1021 tatttgagtg gtagccatca aacttattgg ggaacgagta atgtcgatga gcaattccag
     1081 gctggtttaa atactgcatt cgaagatatc aactggacgc tcagctatag cctgacgaaa
     1141 aacgcctggc aaaaaggacg ggatcagatg tta
//
LOCUS       DAZRUF010000447         1167 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZRUF010000447 DAZRUF010000000
VERSION     DAZRUF010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.450"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(4..1047)
                     /locus_tag="ACHXOL_004363"
     CDS             complement(4..1047)
                     /locus_tag="ACHXOL_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR6811151.1"
                     /translation="MNDRLCFEVHDNKGYFVFSDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT          305 a          274 c          259 g          329 t
ORIGIN      
        1 tatctactga tactggatgc ttgaatgttt catccctgct gcggaaaagg gcaatatcca
       61 gggccagata tattcaccgt tcatatttct gcatttctgt acaatttcct cagaaagtgt
      121 cttgtcaatt cttttccaca ggtgctcctg ctgtcttaaa atactttcgc atatttcgaa
      181 atcatcttcc tgaatcagca tttcggcgca tttaatgatt tctgatcgtt catgcagcac
      241 ggaggaatgg ttatagagcc agtttacaaa cagcaatgct tccggatatt gtccccataa
      301 tggtgagtat gttgcatagt aatcttctgc cgtatccaga gagccggaga agtcatgcca
      361 cacagcaagt ggaaatggat gcagatttcc gcacagcatt tctgcgatgt aagtgtttgt
      421 ggcaaagcca tggcgaaaag ctgtggcggc cttaacatgt tccccgttca gaaaatgcag
      481 gagaccgagt tcataccagt aaggtgaaaa ttcatcagca tgctctttca gtacactgaa
      541 ggctcgctca tgatcgccgg tgcgtaacag ctctgaaccc aacaaccagc gagccccctg
      601 attatcttca ggattgtatg ccagtatttt atcagtgagc ataaccgcat cctgatggcg
      661 ctgtaagtgc acatttgcga gaattgctgc ataaagcgcg cgcagataag gccggttttc
      721 gggatgcatc cagattattt caccacagaa actttccggg atgatacgat tacctgccgc
      781 cagtccttta agagaggcgt tcagtgcctt acgcggggca ttctgttcca gaaatgcata
      841 tgccagatga gcgtgtatat caataaagtc cggctcccgt tgagcaaggc gtcggagttt
      901 gttgatgtaa ctcgtctcgc tgatttcatc agcgtcgtat gcatcaagca cttcttcaaa
      961 ctcccccagc aatgggccaa accaggtatc cgaaaatacg aaataaccct tgttatcgtg
     1021 cacttcaaag cataatctat cgttcatttc tgagcctgta aaacatgtgg tgatatagtc
     1081 tgagagcata aaagttgaga ttctgtgtat caagtcctgt cttggcaccg aatgaatatt
     1141 gcttacgaag ccccttcatg ctttcct
//
LOCUS       DAZRUF010000448         1166 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZRUF010000448 DAZRUF010000000
VERSION     DAZRUF010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.451"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            78..512
                     /locus_tag="ACHXOL_004364"
     CDS             78..512
                     /locus_tag="ACHXOL_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811152.1"
                     /translation="MMSEWKYSDDWSEEEMLSGEYAGFVYIFQFEDGSNYVGAKQIYQ
                     RVKDARKLKPTSKENGWREYLSSSNVVKNKIENGEQYSKTILWCFATMREVMIVEAIL
                     ILHQMLKPECLNLAMMSKIRSPNAKDKKRLLGIVQELLEYLN"
     gene            522..1022
                     /locus_tag="ACHXOL_004365"
     CDS             522..1022
                     /locus_tag="ACHXOL_004365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811153.1"
                     /translation="MASGINGIGSIRNYIQTQGVRLNNQFQNEIIKRSKLLSQRMQGD
                     LNNSVDRGAVGFTQRSILFFYRKVGRNSVTSTIMVKDIQAKYLYQVLVQPKAIDKFIP
                     TSSTRLTKQGNISGLKKNLLSERYKVVKGKNGKERLIDTAKKDTKKKTKRVIGLREKK
                     QRRLIY"
BASE COUNT          413 a          169 c          260 g          324 t
ORIGIN      
        1 gtaccttcat attcttttaa tcgtgttttt aaatcgctca tttactaaat acctataatg
       61 aatttatagg aatatttatg atgagtgaat ggaaatactc ggatgattgg agcgaggagg
      121 aaatgttatc cggtgaatat gctggtttcg tttatatctt tcagtttgag gatggttcta
      181 attatgtcgg tgctaagcaa atctatcagc gggttaagga tgcgagaaag ctaaagccca
      241 cttcaaagga aaatggctgg cgtgagtacc tcagtagttc caatgtcgtt aaaaacaaaa
      301 ttgaaaatgg tgaacagtac tcaaaaacga ttctatggtg cttcgcaact atgcgagagg
      361 taatgattgt agaggcaata ctgatccttc atcagatgct taaaccggaa tgtttgaatc
      421 tggcaatgat gagtaagatt agatcaccaa atgcaaagga taaaaaaaga ctactcggaa
      481 tagtacagga actattggag tacttgaatt gaggtaaata tatggcaagt ggaataaatg
      541 ggattggtag tataaggaac tatatacaaa cgcagggagt tagattaaat aatcaattcc
      601 agaatgaaat aattaaacgt tccaagctac tatcccaaag aatgcagggt gatttgaata
      661 atagcgttga ccgtggggca gtgggcttta ctcaacgttc catccttttc ttctatagaa
      721 aggttgggag aaattcagtt acatcgacca tcatggttaa agatattcag gctaagtacc
      781 tttatcaagt actggtacag ccaaaggcaa ttgataagtt tatacctact tcatctacaa
      841 gattaactaa gcagggcaac atttcaggac tgaaaaagaa tcttttaagt gaacgatata
      901 aggtagtgaa gggtaagaat ggtaaagaac gattaattga taccgcaaag aaagatacca
      961 agaagaaaac caaacgtgta attggcttac gtgagaagaa acaacgtcgc cttatctatt
     1021 gacttctata aggaagccaa tgatggtgta agggtaatta tgagcggtat tcagggaaca
     1081 ttcaggataa gtagacaatg aattttgagc aacattatgg tgatttaata agcaagatta
     1141 agctaaatgg catgacacca gatggt
//
LOCUS       DAZRUF010000449         1163 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZRUF010000449 DAZRUF010000000
VERSION     DAZRUF010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.452"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            248..493
                     /gene="yahM"
                     /locus_tag="ACHXOL_004366"
     CDS             248..493
                     /gene="yahM"
                     /locus_tag="ACHXOL_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR6811154.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..>1163)
                     /locus_tag="ACHXOL_004367"
     CDS             complement(510..>1163)
                     /locus_tag="ACHXOL_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR6811155.1"
                     /translation="LFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGR
                     RAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQ
                     STPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAW
                     AGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          353 a          277 c          275 g          258 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cggggttcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa taa
//
LOCUS       DAZRUF010000450         1160 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZRUF010000450 DAZRUF010000000
VERSION     DAZRUF010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.453"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHXOL_004368"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHXOL_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1160
                     /locus_tag="ACHXOL_004369"
     CDS             572..>1160
                     /locus_tag="ACHXOL_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR6811156.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVG"
BASE COUNT          323 a          260 c          277 g          300 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgggta
//
LOCUS       DAZRUF010000451         1149 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZRUF010000451 DAZRUF010000000
VERSION     DAZRUF010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.454"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            29..1099
                     /locus_tag="ACHXOL_004370"
     CDS             29..1099
                     /locus_tag="ACHXOL_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFR6811157.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGAQISMVSGASIG
                     ALNGAIITASPDLSEAALRLEALWDHLGNNQVLSVNRSVYFSLLKKLVQAMNLCQIPG
                     RAGALLTTLFRHISTINGFDNPMIQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMYGDGGMGGWRNRQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRRAYPDTTILEIRPR
                     KRLKQIGDEGKSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALTRSETVL
                     QKSLEITEGADSALRNAMARIK"
BASE COUNT          267 a          285 c          351 g          246 t
ORIGIN      
        1 ttgatgaaat atttcagaag ggggaggcat gagtacagaa atgaaaacgg ggctggtgct
       61 gtccggcggc ggggcggtgg gcgcttatca ggcgggagtg gttaaggcac tggcagagtg
      121 cggcgcacag atcagtatgg tctcaggagc cagtatcggg gctctcaatg gtgccattat
      181 cacggcctct cccgatctgt cagaagccgc cttacgcctg gaggcgctct gggatcatct
      241 ggggaataat caggttctgt cggtaaacag atcggtttac ttttcattgc tgaaaaaact
      301 ggttcaggcc atgaacctct gccagatccc cggacgtgcg ggcgcactgc ttacgacgct
      361 ttttcgccat atatcgacaa tcaacgggtt cgacaatccg atgattcagc cgctgttgtc
      421 agatgagccc ctgacagcgc tgatggatca ttatcttgat acagatgctc tggccgacgg
      481 gctaccgctg tacgtgtcgc tgtaccccac agaaggaggc atgcaggata ttattgactg
      541 cattcgtgct gaactgggtg ccggaaccac gaaaaacgct gtttttcagc atatccagag
      601 tctgccccgc gggcagcaga aagaagcctt actggcgtca gccgcgctgc ctctgctgtt
      661 ccgtccccgc gaggttcagg gaacaatgta cggtgatggt gggatgggcg gatggcgaaa
      721 caggcagggg aatactcccg tgacgccact ggtggatgcc ggatgcaata tggtgattgt
      781 gacgcatctg agtgacggtt ctttatggga tcgtcgggct tatccggaca ccacaatcct
      841 tgaaatccgt ccccggaaaa ggttgaaaca aatcggggac gaaggcaaaa gtggcggtct
      901 gctcagtttt acatcggcac ataccgacgc ctggcgtcag cagggctacg aggacacgat
      961 gctgacgatg gagcatatcc ggaaaccgct ggcagcacgt caggcactga cccggtcaga
     1021 gacggtattg cagaaaagcc tggagataac tgaaggtgca gattcggcac tgagaaacgc
     1081 gatggcccgg attaaataaa gacgctccgg agaaacgcca ccgccactgt ggtggtgttt
     1141 ttcctgtct
//
LOCUS       DAZRUF010000452         1143 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZRUF010000452 DAZRUF010000000
VERSION     DAZRUF010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.455"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>1143
                     /locus_tag="ACHXOL_004371"
     CDS             <1..>1143
                     /locus_tag="ACHXOL_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR6811158.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVAQMQSQ
                     MDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYDEWGNLLSDENPHHLQQLIR
                     LPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLA
                     SKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSP
                     YDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQTNWSTTQL"
BASE COUNT          291 a          304 c          333 g          215 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct ggcatcgtgc ggcctgacgg tggcgcagat gcaaagccag
      481 atggacccgg tatacacgcc ggcgcgaaaa attcacctgt accactgcga ccatcgcggc
      541 ctgccgctgg cccttatcag cacggaaggg acaacagcgt ggtgcgcaga atatgatgaa
      601 tggggcaacc tgctgagtga tgagaacccg catcatctgc agcagctcat tcgcctgccg
      661 gggcagcagt atgatgagga gtccggcctg tattacaacc gccaccgcta ttatgacccg
      721 ctgcaggggc ggtatatcac tcaggatccg attggactga aggggggatg gaatttttat
      781 cagtatccgt tgaatccggt tcagtatata gattcaatgg ggctggcatc taaatatgga
      841 cacttaaata atggcggata tggagcgaaa cccagcaaac cgcctacgcc cgatccaagt
      901 aagttgccag acatagcgaa acaattaaga ctgccatatc ctattgacca ggccagtagt
      961 gcgcctaatg ttttcaaaac attcttcaga gcattaagcc cttacgatta cacactgtat
     1021 tgcaggaagt gggtaaaacc caatctgact tgtatgccgc aggatgatcc ccagtatcca
     1081 gggatggaga caaagacagc aagtgattac ctgccacaga caaattggtc aacaactcaa
     1141 tta
//
LOCUS       DAZRUF010000453         1140 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZRUF010000453 DAZRUF010000000
VERSION     DAZRUF010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.456"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>1140
                     /locus_tag="ACHXOL_004372"
     CDS             <1..>1140
                     /locus_tag="ACHXOL_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR6811159.1"
                     /translation="EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKES
                     DTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTA
                     EQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAE
                     HTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEALHGYVLTDGKLHVDDTPV
                     PVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVRQ
                     ADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEI
                     RGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFA"
BASE COUNT          250 a          323 c          362 g          205 t
ORIGIN      
        1 ggagcgcgaa atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg
       61 cagtcgttcc gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct
      121 tcagaaagag agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt
      181 gcgtcagacc cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact
      241 gttgcctgcg gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga
      301 taccgccgaa cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga
      361 aaaacatgcc tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat
      421 cgagcggggt atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga
      481 gcacaccccg ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg
      541 ttcactgctg tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc
      601 gcttcatggc tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt
      661 gctgttgcca ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga
      721 ccgtaacgcc gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa
      781 aggcatccat ccgcagaccc atcttgcggg gttcagtggt gtacggcagg cggatgcata
      841 cgccgggttc aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca
      901 cgcccgccgt aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc
      961 gctgaaacgg atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga
     1021 acagcgcctt gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg
     1081 gctgcgtgaa aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata
//
LOCUS       DAZRUF010000454         1135 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZRUF010000454 DAZRUF010000000
VERSION     DAZRUF010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.457"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>1135
                     /locus_tag="ACHXOL_004373"
     CDS             <1..>1135
                     /locus_tag="ACHXOL_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6811160.1"
                     /translation="LINTDTFAETAAYCDAFEYYANGAISYQKTYKDNIENICQSFGG
                     IMYVHAGQICITTDRKSVSVASFDERNMVGAVQVSTSGGTDYFNVVDCKFTNPESMYT
                     TDVVRIPSDITTDEAVQHDGQVIALSRDYSWSYDQEVIAKMANVDVLKAKYALRTISF
                     TTSEGWDLEVWDAINVSNEELAISGKFKVLNKDISTDQENVGYVTITAVEAPDAMYDG
                     IDPGIWSPGGVINFPELQVLPPTNLQAVRKGNTTSGSIVDLTWDASPDPYLRGYYVYY
                     RLNGSSTWTYAGQTGVQKLDYELFGLSDTASYDFSVEAYNNLGLVSKKLSLTGIVPTF
                     NFALPAVTRVVLTNKTESAYVTDSPDFNIAWDSQKDIRVNNRSF"
BASE COUNT          332 a          239 c          253 g          311 t
ORIGIN      
        1 ttgattaata ccgatacgtt tgcagaaacc gcagcatatt gtgatgcttt tgaatattat
       61 gctaatggtg ctatcagtta tcaaaaaacc tataaagata atattgaaaa tatctgtcag
      121 tcatttggcg gcatcatgta tgtacatgcc ggacaaatct gtattaccac agaccgcaaa
      181 tcggtatccg tagcctcatt tgatgagcgt aacatggtag gggcggtaca ggtttctact
      241 tctggcggta ctgactactt caacgtagtc gattgcaagt ttactaaccc tgaatcgatg
      301 tacaccactg acgtagtacg tatcccttcc gatatcacta cggatgaagc agtacagcat
      361 gatggacagg tcatagcatt atcacgcgat tactcatggt catatgatca agaggtgatt
      421 gcaaaaatgg ctaacgttga tgtactcaag gctaagtatg ccctgcgtac tatcagcttc
      481 accacaagtg aaggttggga tctcgaagta tgggatgcga tcaatgtcag caatgaagaa
      541 ctcgctatct caggcaaatt taaagtactc aataaagata tcagcaccga tcaagagaac
      601 gttggctatg taaccatcac ggcagtagaa gcacctgatg caatgtatga tggtattgat
      661 ccgggtatct ggtcgcctgg tggggtaatt aacttccctg aactacaggt actaccgcca
      721 acgaacctac aggcagtacg taaggggaat actacttctg gttcaatcgt ggatcttacc
      781 tgggatgctt caccagatcc gtatctgcgt gggtactatg tttactaccg tcttaacggt
      841 tccagtacct ggacgtatgc ggggcagact ggcgtacaga agcttgatta tgaattattt
      901 ggattgtctg atactgcaag ctacgacttt tcggtagagg cttacaacaa cttagggctg
      961 gtatccaaga agctttccct aactggcatt gttccaacat tcaactttgc actacctgcg
     1021 gtaacgcgtg tagtactgac aaataaaaca gaatcagcgt atgtaactga ctcaccagat
     1081 ttcaatattg cctgggattc acaaaaagat atcagagtga ataaccgatc attta
//
LOCUS       DAZRUF010000455         1115 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZRUF010000455 DAZRUF010000000
VERSION     DAZRUF010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.458"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            87..194
                     /gene="yniD"
                     /locus_tag="ACHXOL_004374"
     CDS             87..194
                     /gene="yniD"
                     /locus_tag="ACHXOL_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR6811161.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(247..1005)
                     /locus_tag="ACHXOL_004375"
     CDS             complement(247..1005)
                     /locus_tag="ACHXOL_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR6811162.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          309 a          272 c          265 g          269 t
ORIGIN      
        1 acggctgtaa taacaaaaaa tcccttgatg cctatccctt ttgttacact ccgttatcac
       61 gcacaagaga tatgcaggac actggtatgc cgactaaacg ctttgataaa aaacactgga
      121 agatggtggt ggtgctactg gcaatctgtg gcgctatgtt gttgctacgt tgggcagcaa
      181 tgatttgggg ctgagcagtg acaaacgaag acagccagac cgcataacac ggtccggcag
      241 ataattttac atgctgtagc ccagcgataa ggttgtccga cgatcggtat gttccggtgc
      301 agactctgga ggttcagagt tccatgtgac gttgtaagcc accttcagtc caaaatgttc
      361 attaatagca acatttaatg cgctttcgga gttcagcgtt gtatcttccg cgccaaagac
      421 cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc catgcataag ccccggaggc
      481 atagcccagc ggctgggttt cactggcatt gtcggtatat ttgtcgtaac gcacacctgg
      541 gccgaattca aagcggaaac tgtgtaccgg gccattcaaa aactggcgac cataacccgc
      601 ggtcaacaca tcgcgctctc gatagccgtt ataacggtct gtcagccagc ttgcctgacc
      661 aaataaatag tcataatcag ttaaattaaa acggctacgc ccgcccgccg catatttttc
      721 tgaagaacgc tcatcattag aagaagtatt actggcgttc ccccacagcg accaggcagt
      781 ggtttgtcca taccaggtca tggtggtgtc agccgtaagt gaggagcttt tcgtattgcc
      841 tgattgtgca agatatcctg cgttcagatt accttcgaaa ggttttttag cgctggcagg
      901 atcgtccatg acagtaaaaa cggaatcatc ggcagctgca ttcagtgacg caaacatgcc
      961 ccccgccaac ataacgatgg caggaactgt cttcaaaagc ttcatttatc aagagtccgt
     1021 acaacaaaaa aagagaccat cgcggtcccg gaaactttct taaggatcaa agattagcgt
     1081 ccctggaaag gtaacgaatt ataaaaaggc gcgaa
//
LOCUS       DAZRUF010000456         1113 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZRUF010000456 DAZRUF010000000
VERSION     DAZRUF010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.459"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..477)
                     /gene="umuC"
                     /locus_tag="ACHXOL_004376"
     CDS             complement(<1..477)
                     /gene="umuC"
                     /locus_tag="ACHXOL_004376"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR6811163.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
     gene            complement(477..896)
                     /locus_tag="ACHXOL_004377"
     CDS             complement(477..896)
                     /locus_tag="ACHXOL_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR6811164.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT          313 a          291 c          235 g          274 t
ORIGIN      
        1 ctgccgctgc catttttttg ccgcatgatt ggcaagctta gccagcgttt tggtctgggc
       61 gatccccata ccaacagtaa gatgggtacg ttgtagcacc gttgcgcgaa tttctctgcc
      121 aaaatcagtc agatcgcgac aattacgcac acctgtcaga tcgcagaatg cctcatcaat
      181 actgtaaatc tcgacgcggg gcgatagttc ttccagcgtc gacatcaccc gattgctcat
      241 gtctgcgtaa agctcataat tgctgctaaa gcaaaccaca ccacagcgac gaaacagatc
      301 tttttgtttg aaccagggat cgcccatttt aacacccagc gcctttgcct cagcgtttcg
      361 ggcgataacg caaccgtcat tattcgatag cacaaccacc ggtttacccc ataaatcagg
      421 gcgaaacacc gtctcacagc tggcataaaa cgcgtttaca tcacagagag caaacatcag
      481 cgcatcgcct taacgacgtg gatcaccaca ccaaagacat ccagcgtatc ttcgctactg
      541 atggtaatgg gcgagtacgc actgttcatg ggaataagct gtaccgtcgg gcgtagttgc
      601 aattttttca ccgtaaactc gccgtcaaca gcagcgatga caatatcacc atggctggcg
      661 gtaatagcgc tatcgacaat cagtaaatca ccgtcactaa ttccaccatc aatcatagaa
      721 tcaccacttg ctttgacgaa gtaagtcgcg ctgggatgct ggatcaacag ttgattcaga
      781 tcgatgcgct gttcgacgta atctgctgcc ggtgaaggaa agccacactg aacaagatcg
      841 ctaaatagcg gaaaagtcac aatttcgcgg agatccgcag gcttgataaa caacataata
      901 atctgcctga agttatactg tttttatata cagtaatctg ttcttgccag cagatcaata
      961 ctgattcagg ctatcaatat ttgtcgctgc atagactgct gatttttcgt tctcttatcc
     1021 tgtgctcacg tggccttctg gcgacgacgc tcatccagca gaaatgaaaa atatcacccg
     1081 gcaaaaaaat agaatagaag cattcgccat aat
//
LOCUS       DAZRUF010000457         1091 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZRUF010000457 DAZRUF010000000
VERSION     DAZRUF010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.460"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..265)
                     /locus_tag="ACHXOL_004378"
     CDS             complement(<1..265)
                     /locus_tag="ACHXOL_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR6811165.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEG"
     gene            complement(300..1040)
                     /locus_tag="ACHXOL_004379"
     CDS             complement(300..1040)
                     /locus_tag="ACHXOL_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR6811166.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
BASE COUNT          313 a          233 c          229 g          316 t
ORIGIN      
        1 ttccttcaat ttcaataaat gtaatgcttt ggttaataat aggttgatcg ttgacataaa
       61 cactgacgtc gtacataccc ggtatagcag ggttaccttc actataccgg ctgagatcaa
      121 tatttgaggc attctgcccc atcaggaagg tatggtcata ttcaacaagt tcagcacaga
      181 aagcactatt gcaatacagc agcgcgcaaa acgtagcgat acgggtcaga tgattatgat
      241 aatttttagt tggttttata gtcacgttat gcttcctgcg gaattatatc cttgcctgat
      301 tacagactgg cattaccttc aatggcgcca ccaaaatcat taatggcgta aaaatgtact
      361 tttgcagaac ttgctttgcc attaaggcca gtgactttca tgacctcatc actaaatggt
      421 gcaatcattt tcacatcaat cggatagcgt ttaccgctag cttctaaatc accactgcta
      481 aaagagacgt agtaaggggt tggattggtc actcgcaatg acgccttacc ttctgaacct
      541 gaccagaacc acttcagggc taacggggct tcagagggat ttcccttcaa tccatccggg
      601 cgatagaaaa gttttatacg tgtgcgaaat gccagttgca gcagactttg attcgcgacc
      661 ttttctgcat ctggttttgg tggaacttcc agtacgttaa accagaacac gctctctctg
      721 tctttaggca gtgaggtgct ggctgtgtac attaatttga ttgtttgccc acgtttggca
      781 tcaatacgcg atactggcgg cgtggcagta aaagggactg taatactgcc aggctcagcg
      841 ttgttatcgc cagtatctaa ccaactctgg acaagcaacg ggttattccc tttattttcc
      901 agacgtacgt tgacactttt ttgatcgctt ttatatatta cgcgagtacc cgaaatgaca
      961 atgtccgcaa tagacgatga actaaaagcc atacaggtta cgaagcataa agctgttgta
     1021 tgtttggtgt taaaaaacat aaaccatccc tgacagagtt aattaaaaca gggaataata
     1081 taaaaataga a
//
LOCUS       DAZRUF010000458         1090 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZRUF010000458 DAZRUF010000000
VERSION     DAZRUF010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.461"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>1090
                     /locus_tag="ACHXOL_004380"
     CDS             <1..>1090
                     /locus_tag="ACHXOL_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR6811167.1"
                     /translation="LLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGTF
                     ILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLDQ
                     IKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGLY
                     LDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTSS
                     VALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITLQ
                     LDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGIK
                     RYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT          366 a          210 c          242 g          272 t
ORIGIN      
        1 gtcttctgat tcactcagcc aaattaaaaa atgaaataaa gagccactat accaaagacg
       61 aaattcaagg gttgttaaca ttaacagaaa acacacgaaa gttaacgttg acggagaaac
      121 cctggggaac atttattctt gcttctactt tcgaagatga caaaacagcg gcagaaaccc
      181 actatgatgc tgtctggctg agggatagtt tatggggcta tatggcatta gtatcagatc
      241 agggaaatag tgtagccgca aaaaaggttc tgctcaccct gtgggattat atgtctacgc
      301 tcgaccagat taagcggatg caggacgtca ttagcaaccc caaacgactg gatggagttc
      361 ccgggcaaat gaatgctgtg catatccgat ttgacagcaa ctcccccgtg atggcagatg
      421 tgcaggaaga aggcaagccg cagctgtgga accataaaca aaatgatgcg ttaggtcttt
      481 atcttgatct ccttatacaa gcaatcgaca ctggtacaat taacgctgaa gattggcaaa
      541 aaggtgaccg attaaaatcg gttgcgctgc tgattgctta tctggataaa gccaatttct
      601 atgttatgga agattccgga gcatgggaag aagatgcccg attaaacaca tcttccgttg
      661 cactggtgac ctctggactg gaacgtttgt cgaacttact ctcgaagaaa gactctgttt
      721 ttgtctctga tttactacgc gaagctaaag ccaatgaact ggatgaacct ctttcaacaa
      781 cgaggttgaa ccacctcata gataaaggtt atgaaagaat aacgttacag cttgatttag
      841 ggggagaatc cccaggttat ctggaaaaag ataaacatta cagagaagcc gatgcggctt
      901 tacttaatgt tatttatcct gcaaatcttg caaagataaa caccagaaga aaagagcagg
      961 ttctaaaaat agttaaaaaa ctagcaggtc cgtatggtat aaaaagatac gagaaggata
     1021 attatcagtc ggcaaatttt tggttcaatg atattaaaac cgataccgat caaaattcac
     1081 atgcgaaaag
//
LOCUS       DAZRUF010000459         1087 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZRUF010000459 DAZRUF010000000
VERSION     DAZRUF010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.462"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            35..190
                     /locus_tag="ACHXOL_004381"
                     /pseudo
     CDS             35..190
                     /locus_tag="ACHXOL_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            294..>1087
                     /locus_tag="ACHXOL_004382"
     CDS             294..>1087
                     /locus_tag="ACHXOL_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6811168.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSL"
BASE COUNT          315 a          223 c          254 g          295 t
ORIGIN      
        1 ataacagatg tctggaaata taggggcaaa tccaggtact gccgaaaata ttcagataga
       61 gctgagggat gaccaggatg ctgcgttaaa aaatggcgat agcaaaacgg ttattgttga
      121 tgagatcact cgtaatgcac agtttccact taaggcaaga gctatcacgg tgaatggaaa
      181 cgcaagctag ggaacgatcg aggcgctaat caatgtgatc tacacctggc aataacgtcg
      241 attcgatttt cttagccttc tttttaaatc aatttttaac cttaggatat atcatgggta
      301 aaacaatcag tataaaggtc ctgttcggta tttatcttct attaatggca ggcaaagttt
      361 ttgccttcag ttgtaatgtc gatggcgggt caagtattgg agcaggtaca acctctgttt
      421 atgttaatct cgaccctgta atacagccgg gccagaatct ggttgtagac ttgtctcagc
      481 atataagttg ctggaatgat tacggcggct ggtacgacac tgatcatata aacctggtac
      541 aaggctcagc tttcgcaggc tccctacagt catataaagg atcgctgtac tggaataatg
      601 tgacctaccc cttcccgttg acgacaaata ccaacgtatt ggatattggt gataagacac
      661 cgatgccatt accgttaaaa ctctatatta ccccagttgg ggcggccggt ggagttgtca
      721 taaaagcggg ggaagtcatt gcccgtattc atatgtataa aattgcaaca ttaggaagcg
      781 gaaatccgcg taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag
      841 gcggctgcac cgttgatagt cgtaatgtca ctgtggatct cccggacttt cctggaagcg
      901 cagaaatacc ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg
      961 gtgccaccac tgacagtagc cgacaagtgt ttgctaacac agcacctgat gcaacaaaag
     1021 catcgggagt gggtgtcact ctaatgcgta atggaaaaat actggctacg ggagaaaatg
     1081 tctccct
//
LOCUS       DAZRUF010000460         1081 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZRUF010000460 DAZRUF010000000
VERSION     DAZRUF010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.463"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            72..245
                     /gene="ymgI"
                     /locus_tag="ACHXOL_004383"
     CDS             72..245
                     /gene="ymgI"
                     /locus_tag="ACHXOL_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR6811169.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            247..591
                     /locus_tag="ACHXOL_004384"
     CDS             247..591
                     /locus_tag="ACHXOL_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR6811170.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            595..930
                     /locus_tag="ACHXOL_004385"
     CDS             595..930
                     /locus_tag="ACHXOL_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR6811171.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
BASE COUNT          313 a          227 c          260 g          281 t
ORIGIN      
        1 cagattatct tacctttaca acattgcgtt aacagtcaat gatacttctt gaataaaaat
       61 attgccaatc aatgagttat tcctcattta agattattct cattcacgta aaaaacataa
      121 ttcctatcat cacagccacg ctcattttga attatctgaa taatagcgag cgttctttag
      181 ttaagcagat cttaattgaa gacgaaatta ttgtgtgtgc aacttactta ataccggaca
      241 tctaagttga agaaaaaaat acttgccttc gggcttatct cagcattgtt ctgctccacg
      301 ccagcgatgg cggatatgaa ccgcaccacg aaaggtgcat tgctaggcgc aggcgttgga
      361 ttacttactg gcaatggtgt taatggcgta ctgaaaggtg cagctgttgg cgctggtgtt
      421 ggtgcagtaa cagaaaaagg ccgcgacggt aaaaatgcac gtaaaggtgc aaaagtgggt
      481 gccgccgttg gtgctgtcac tggcgttctg accggcaatg gcctcgaagg ggcgattaaa
      541 ggtgccgtga ttggcggtac gggtggtgca attctgggta aaatgaaata aggaatgatg
      601 atgaaaaaat ttgctttact ggcgggcctg tttgttttcg caccaatgac ctgggcgcag
      661 gactacaata ttaaaaacgg tttgccgtca gaaacctata ttacttgcgc agaagccaac
      721 gaaatggcaa aaactgacag cgcacaggtt gcagaaattg ttgcggtgat gggtaatgcc
      781 agcgttgcca gccgtgattt aaaaattgag caatctccgg aactgagcgc caaagtggta
      841 gaaaaactga atcaggtttg cgctaaagac ccgcaaatgc tgttgatcac ggcaattgat
      901 gacaccatgc gtgctatcgg caaaaaataa tatttaatat gttaccgcgt cgatgaattc
      961 gacgcggtat tttagctaac ggggatcaga acttatattt caggcccacc atcactgcgg
     1021 tgtcattgta gccattatca cccagctgca cgcccacatt accccagaca ctggccgccg
     1081 g
//
LOCUS       DAZRUF010000461         1080 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZRUF010000461 DAZRUF010000000
VERSION     DAZRUF010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.464"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            122..>1080
                     /locus_tag="ACHXOL_004386"
     CDS             122..>1080
                     /locus_tag="ACHXOL_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR6811172.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
BASE COUNT          354 a          270 c          167 g          289 t
ORIGIN      
        1 caaactatat atccgcctcc tccagaagtt cctgtctact gctgaaggga accaataaca
       61 ttgcaaagca gataaaaaag aatccaacac ctctcaacat aaagaatacg ccctgatatt
      121 tatgctcccg acaaataaca atcacagatt aatttcaaat tcgttctcca cttattcaat
      181 cgacactagc cgtgcatatg aaaattatct aacccattgg actgaatgga aaaataaccg
      241 catacaagaa gaacaacgag acatcgcttt tcagcgacta gtatcatgtc tacaaaacca
      301 agagacgaac ctggacttgt ctgaattagg cctgacaaca ttacctgaaa tccccccggg
      361 aattaaatca attaatataa gtaaaaataa tttaagctta atctccccat tgcctgcgtc
      421 ccttacacag cttaatgtca gctataacag acttattgaa ctgcctgctt tgcctcaagg
      481 acttaaatta ttgaatgcgt cccacaatca actaatcaca ctacccacac tccccatatc
      541 tttgaaggag cttcatgtct caaataatca attatgttct cttcctgttt taccagaact
      601 actggaaaca ttagatgtat catgtaatgg gctggcagtt ttaccacctt taccattttc
      661 tttacaagag attagcgcaa tagggaatct tcttagtgaa ctcccccctc tacctcacaa
      721 cattcactcc atatgggcaa tcgacaatat gttaaccgat attccatacc tgccggaaaa
      781 tttaaggaac ggttattttg acataaatca gataagtcat atcccggaaa gcattcttaa
      841 tctgaggaat gaatgttcaa tagatattag tgataaccca ttgtcatccc atgctctgca
      901 atccctgcaa agattaacct cttcgccgga ctaccacggc ccgcagattt acttctccat
      961 gagtgacgga caacagaata cactccatcg ccccctggct gatgccgtga cagcatggtt
     1021 cccggaaaac aaacaatctg atgtatcaca gatatggcat gcttttgaac atgaagagca
//
LOCUS       DAZRUF010000462         1074 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZRUF010000462 DAZRUF010000000
VERSION     DAZRUF010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.465"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            44..>1074
                     /locus_tag="ACHXOL_004387"
     CDS             44..>1074
                     /locus_tag="ACHXOL_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR6811173.1"
                     /translation="MPQLKGVIKTPTGEPLDGATITLTSIHNRAGILKSVFSHVTTQN
                     GEYDFPVLPGVYSVRLTQSTRRLSEIGVIRVYEDSTDGSLNDFLGATNIDLRPESLKK
                     FEELAQQAQQSAGSAAGNARQTAQDVTAAATARDDAQRFAEKARQDATVTAENRKATA
                     EDVKSTGKNAVLSGQRAQAAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAGEKA
                     QQKSRDNLRLKSAATMEAQSDIYDRTKGRLAIPGAFGFGRAFLPEDVIRFDTKSDFLA
                     WVRNALPGEYSVAGPYDIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGINGP
                     IYHTRYCYWP"
BASE COUNT          285 a          295 c          296 g          198 t
ORIGIN      
        1 gataaagcca catataacgc catcaactgg ccggagcgtc ccgatgccgc agcttaaagg
       61 tgtgattaaa acgcccacgg gagaaccgct ggacggcgca accattacac taacctccat
      121 acacaaccgt gcagggattc tgaaaagtgt tttcagccat gtcaccacac agaacgggga
      181 atatgacttc cccgttctgc cgggtgtgta cagcgttcgc ctgacacaaa gcacacggcg
      241 cctttcagaa atcggtgtca tccgcgttta tgaagattca acggacggtt cgctgaatga
      301 ttttctgggc gcaactaata ttgacctgcg tccggaatcc ctgaagaaat tcgaagagct
      361 ggcgcaacag gcgcagcaga gtgccgggag cgcagccgga aacgcacggc agacggcgca
      421 ggatgtgacg gcagccgcaa cggcccgtga tgatgcacaa cgttttgcgg agaaagcccg
      481 acaggatgca accgtcacag ctgagaacag aaaggccaca gcggaagacg tgaaaagcac
      541 ggggaaaaac gccgtcttat ccggtcagcg tgcacaggct gccgcaggtt atgcccgcgc
      601 agcagaacag gccaaaaatg acattgatgc tgcgctgact ggcactctga aaatggctaa
      661 ccatctgtca gaaatcgcag cagcaggcga aaaggcacaa cagaagtccc gggataatct
      721 gaggctgaaa agtgcggcca cgatggaagc acagagcgac atttacgacc ggacaaaagg
      781 ccgtctggcg atacccggcg cattcggctt tgggcgtgct tttctgcctg aagatgttat
      841 ccgttttgac actaagagtg atttcctggc ctgggtaagg aatgcgctgc caggtgagta
      901 ttccgttgct ggcccctacg acatcatcat acccgacaca cggtttgaag gggtgctcag
      961 catccggtgg actgatgcac gccctgagac aacagaaccg cggtacagag ccaaatccct
     1021 tactttttac ggcattaacg gccccattta tcacacccgc tactgctact ggcc
//
LOCUS       DAZRUF010000463         1067 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZRUF010000463 DAZRUF010000000
VERSION     DAZRUF010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.466"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..649)
                     /locus_tag="ACHXOL_004388"
     CDS             complement(<1..649)
                     /locus_tag="ACHXOL_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811174.1"
                     /translation="MAGFEVYNSAGALTIDSTNKSIMTGAVKAMGNLTDTGYYTGFTC
                     AFGNGGSLGFVMPSVVANRNTTQYWFQIQKDGAWCFPGAYMFQPGMGRFMTSSHTATP
                     TSGFLDVFSEEGTLIWSAASAATMPRIRGFLTAPAATDLSTAITVTSPVADPWFCWSQ
                     CPGNISDDGTVIGYSGLVIRRNSSTSFSLQYVSKNQKTYRQAMGNNGIQIALATFT"
     gene            complement(651..947)
                     /locus_tag="ACHXOL_004389"
     CDS             complement(651..947)
                     /locus_tag="ACHXOL_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811175.1"
                     /translation="MAQGLQCWNAAGVLVVDLTDYNIRFMGTYTVKATSAQTYTVSVP
                     NMKTTGWFVHYAPASDTFNEWSAFCNNGSFTAVYLPTYAPLAGNYTFNVYKWTA"
BASE COUNT          290 a          254 c          216 g          307 t
ORIGIN      
        1 ctgtaaatgt cgccaatgct atctggatac cattattccc cattgcctgg cggtaggttt
       61 tctggttctt tgaaacgtac tgtagactga atgaagtact ggaattacgc ctgataacca
      121 gaccagagta cccaattact gtaccatcat ctgatatgtt ccccggacat tgtgaccagc
      181 agaaccaggg atcagctact ggagacgtta ccgtaatggc agtactgaga tcggtagccg
      241 caggtgctgt caaaaatccc ctgatacgtg gcattgttgc cgctgatgca gcagaccaga
      301 ttaaagtacc ttcttcactg aacacatcaa gaaaaccaga tgtgggggta gccgtgtgac
      361 tacttgtcat gaacctgccc atgccaggct ggaacatata ggcaccaggg aaacaccatg
      421 ccccgtcttt ctgtatctgg aaccagtact gtgttgtgtt cctgttagct actactgatg
      481 gcataacgaa accgagcgat ccaccattac cgaaggcaca ggtaaagcct gtgtagtatc
      541 ccgtatcagt tagatttccc atggccttta ctgcacccgt cattatcgac ttgttagtac
      601 tatctatagt cagtgcaccc gctgaattat atacctcgaa acctgccatc ttacgccgtc
      661 cacttatata cgttaaaagt gtagttacca gctaacggtg catatgttgg caaataaacc
      721 gcagtaaatg acccattatt acaaaaggct gaccactcat taaaggtatc actggcgggg
      781 gcatagtgta caaaccagcc tgtagttttc atatttggta ctgatacagt ataggtctgt
      841 gcagaagtag ccttgacagt ataagtaccc ataaaacgta tgttataatc agtcagatct
      901 actactaata cacccgctgc attccaacat tgtaatccct gtgccatagc tcttactcct
      961 gtaaatcctt ttcttcattc caattgtttc ttttccgcta atactgaatg ggttagtact
     1021 ggtactattg aaagtacgtt cattagattt catttatgta tcctttt
//
LOCUS       DAZRUF010000464         1060 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZRUF010000464 DAZRUF010000000
VERSION     DAZRUF010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.467"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            380..910
                     /locus_tag="ACHXOL_004390"
                     /pseudo
     CDS             380..910
                     /locus_tag="ACHXOL_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
BASE COUNT          327 a          233 c          199 g          301 t
ORIGIN      
        1 tagcgaagaa gtggtcgagg aaatgatgtc tcattgagta ttctcatgat aacgcctcga
       61 tgccgcttta gtaagtcatc ataactgcca ctggtccatc cacaaacgcc actgaacgca
      121 agctagctac agacacgctc atcactatga cgtgtctgta tattaataag ctaacccgca
      181 ttgagttaac caataacgga ttccatacac aatacggcca acagagaaag atacctgtgc
      241 tcacgccatt gcttatattg gctggttaca atgtgcacta tcaatttttt aaataacaaa
      301 ttcttaaaac cacatctgta taaggaattt ttaaggttcg tgggatagct tgactgtaaa
      361 aattacagga gctacaaaaa tgaaccgatt ctcaaaaact caaatttatt tacattggat
      421 aacgctgctt ttcgttgcaa taacctatgc cgcgatggaa ctccgtggct ggtttcctaa
      481 aggtagtagt acttatctgc tgatgcgaga aacacattac aatgcgggta tattcgtttg
      541 ggtgttaatg ttttcacgcc tgattataaa acaccgttat agtgatcctt ctattgtacc
      601 accgccacct gcctggcaaa tgaaagcggc ttcgctaatg cacatcatgc tttatataac
      661 cttccttgca cttcctctgc tggggattgc tttgatggct tacagtggaa aatcgtggag
      721 tttccttggt ttcaatgtgt ctccctttgt taccccaaac agcgaaatta aagcactgat
      781 aaaaaatatt cacgaaacct aggcaaatat aggttacttt ttaatcgcag ctcacgctgg
      841 cgcagcactc tttcatcact acattcagaa agataatact ctgttacgaa tgatgcatcg
      901 ccgcaaataa gtcgatcccc tgacaaggaa atgtgaacca tgtacatcaa cgcgtcataa
      961 atgtttacgc aagtgcacaa tgcatccttt ctactatcaa caaggaggag cagaaactgt
     1021 gtaaatcgta caatggtaag acttatcaaa aatacaggtt
//
LOCUS       DAZRUF010000465         1059 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZRUF010000465 DAZRUF010000000
VERSION     DAZRUF010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.468"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(232..855)
                     /locus_tag="ACHXOL_004391"
     CDS             complement(232..855)
                     /locus_tag="ACHXOL_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR6811176.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          276 a          232 c          216 g          335 t
ORIGIN      
        1 tacaagtaag agtatgatta cgctttttaa agcgtaccat aaagcataac ataataaatg
       61 atttgtgatg acactggatt attgcggttt actttgagga aaataaaatg cgtgtttata
      121 gaaccgcgag cgccaaataa ttttatgacc acaacctgtc gtattgtggt cataaaaaat
      181 tgtcggctgg agggagttaa atcctttacc tcaatatact gcctgtcagc attagttcac
      241 aaacagtgct tcgctatcga cgatgattcc atcttccgca cctttaagcg ccaggtttgc
      301 ctcgtagtat tgctttttat caagcagttt ctgcgcatca gcaagtgcat tacgagtctg
      361 tttaagcggc atcagatatt ggttttccat tacgccaaca ccggccaggc gaagctcttc
      421 cattgcgccc tttttatccc ctttcgccat tttttcattg gcaattttta tcgcggcttc
      481 tttttcgggt gttgctacat aactctcaga aattccgaca gatgcattaa tgacaatata
      541 ttggtcatca ttgagattgg tctttttacc tggcttagcg aactttgccc attcggtact
      601 gtcatctgac agcaaagcgg aagcttcatt ggtcagttct tttgcctttt ccggatcgcc
      661 atgaaacaga gccaggcgcg ctacctgcac gtcacgcatc gcatataaac cttgttcaga
      721 gatacgctct gctgctgccg ttttctgggt ttgctgagtt tgctgttgtg ccgctaccgg
      781 tgcattatca gcagcccata cagttgaaga tgccagaatt gctgtgacca tcgtagccat
      841 aattaaacgt ttcatactta cctcattctt taacagtttt agaaaacgcc gccatcatat
      901 tgggtgagca tgaaatgaga aatcagagag caacggtcat tttgaaaggg ttatcttaca
      961 attgtagtta ttatcgcaaa gcggcattta gtcttacagt tgtaggaata tgcttcctgt
     1021 cgcagagaac tattcttata taccacaaag agatgtcat
//
LOCUS       DAZRUF010000466         1054 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZRUF010000466 DAZRUF010000000
VERSION     DAZRUF010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.469"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..120
                     /locus_tag="ACHXOL_004392"
     CDS             <1..120
                     /locus_tag="ACHXOL_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR6811177.1"
                     /translation="EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            120..410
                     /locus_tag="ACHXOL_004393"
     CDS             120..410
                     /locus_tag="ACHXOL_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR6811178.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            407..961
                     /locus_tag="ACHXOL_004394"
     CDS             407..961
                     /locus_tag="ACHXOL_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR6811179.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          299 a          202 c          301 g          252 t
ORIGIN      
        1 gagtttcagc aggtttataa atctgttctg aatgtgctgt ggaactggat tctgttccgt
       61 aaattttcct ctccggaaca agtcgaaaat gtggccgcgc agctgctgga gtttgcgtaa
      121 tggtggattt acgtaaagcg gcgcgggggc agatgtgcac cgtcagaatt cctggctact
      181 gcaatcacga tccggaaacg tctgtgctgg cgcattaccg actggcggga acgtgcggaa
      241 cagcgataaa gccacacgat atgcaggcag cgattgcctg tagctcgtgc cacgatttaa
      301 tcgacgggcg ggtaaaaacc agcgattaca ccaaagaaga attacgcctg atgcatgcag
      361 aaggtgtttt tcgcacacaa gaaatctgga gaaaggaggg atatttatga tttacccaac
      421 gaatacagga aaaagcggag aacaccttcg tctcaccacg ctggaaagtg tctggattca
      481 gggaaaacta cgtatgtggg ggcgctggtc gtatattggt ggcggtaaga cggggaatat
      541 gtttaacctg atgttgacct ctaaaaagct gacaaaaacg gcaattaacg aggcgctccg
      601 gaggatgaaa aaagcaggtc tgaacaagtc tgaacttgag gcttttttgc gggatatgat
      661 taacggtaag caaaagagct ggctggcgca ttgtactgat gcagaggcgt tatgtattga
      721 tcgggtcata agtgaggtgc tggcagagca tccaggattg attagcgtcc ttcggcaacg
      781 gtatgagggg cgggggatga ccaaacgcaa aatggctgaa ctgctgaatg atgcacaccc
      841 gaaatggagt ttaagaacct gtgaaagacg cattgagcat tggctaaagg tggcagaatt
      901 tattttgtac aaaccaatgg ttatggcttt tggtatagag aaaaaagtta ttgctttttg
      961 acgtaaaaac tgcttcaatt cttgtacgct tcgcaaagct gtaccgcgag gcgaatagca
     1021 gacatggaca tttgaaagag cccgctttat gcgg
//
LOCUS       DAZRUF010000467         1048 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZRUF010000467 DAZRUF010000000
VERSION     DAZRUF010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.470"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(1..374)
                     /locus_tag="ACHXOL_004395"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="ACHXOL_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
BASE COUNT          270 a          266 c          231 g          281 t
ORIGIN      
        1 cgcgctgagg cgctcaaatc gagataatca gccagttcat cgagggtata ggcttcctct
       61 cgccactcca tcacctgcca ttccaggtgg aacggatttt tacaaatcag tccgctgtag
      121 ttcacatcag cacaaagttt ctcacacagc gaacgttcaa tcgcagcggc gtatttcagc
      181 gccttaaccg agctgtccgg cgcggttctc acggcgatat tgagcgcgta gagcaggtga
      241 gcatgaccgt tcacaggatt ttttacggtg atgttcggcg cgggagcgtt ccggtcgctc
      301 cagtcaatcg ctgccccctc gcgatcaacg tcaaaaacca gccagaactg cgcatgaggc
      361 tggttctgcc ggatctggac atgatttcag gggcgtttgt ttttcagtcg cttacactac
      421 acccaacggc aaatcctgtc gaaccttccc gaaaacccga tccatttacc ataactcccg
      481 ttgtacgtgc cctctcagac gctctctgac gcaaaaaact caaaaagaca agaaaaaaca
      541 gggtaaacat aaaaaatgcg tcagaatcgt ttttagcgcg ttgcataact atgcacgaaa
      601 gcgacccgcg atcggttttg tgtgttctga ataccgaacg ggtcaaaatg acacgttttc
      661 tctccctcaa tggagcgttc tatacagaga tttttgttcc cctatccatt attctgatta
      721 ccgggtttcc aagctgaaaa caggactttt ctgcgaccag tttttgcgaa tatagtcaca
      781 taaaccaccg attgagtgac agttactgaa acaactattt cgacacaaaa tctcgtctat
      841 tcaacatcat gcaaatttgt cagtgactga atttagtgac agaaccctct ggaattctgc
      901 cgtgtaacca cttatatgca gacctgcccc gttgctctgg gctttcaggc tgcgatgagt
      961 gatgagcgat gatttccata cagatgatat atcgactgga attgattgcc tgcaattaga
     1021 tttagtttta ttgtgtggat cgttttag
//
LOCUS       DAZRUF010000468         1046 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZRUF010000468 DAZRUF010000000
VERSION     DAZRUF010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.471"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(23..832)
                     /gene="pduF"
                     /locus_tag="ACHXOL_004396"
     CDS             complement(23..832)
                     /gene="pduF"
                     /locus_tag="ACHXOL_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR6811180.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
BASE COUNT          340 a          264 c          209 g          233 t
ORIGIN      
        1 ttataactgc cagagtattt cgttattcgc taattccttt aggtgtaaca ctattgacag
       61 gtaaattctt tgcaatgaga tagcgataaa tcgccgcacc aacacatgcc ccaatgatcg
      121 gtgcaataat gggtacaatg aaataaggaa tattgcgtcc accagtcatc gcaatttctc
      181 cccaccccgc aagccaggtg aaaatcttag gtccaaaatc acgcgctgga ttcatggcaa
      241 aaccggttaa tggtccggta gaagcaccaa taacagcaac cagaatacca ataagtaaag
      301 gtgcaagcgg tcctttgggt acgccattac cgtcatcggt caacgccata atcaacccca
      361 ttaacatgga cgttatgacc acttcgacca acgcagcttg ccatacatta atcgccgctg
      421 ccggataagt actgaagata ctggcgagtt gtaaactttc tgcgctacca cgcaccatat
      481 tgtgagcaga ttcaaaatcg gtaaacagac tactgtatag agaatatgcc agaactgcac
      541 caccaaaggc acccgctaat tgcgccagac aaaatggcag cactttacgg gctgggaaag
      601 aggcaaacag ccacagagca atggtaactg ccgggttgag atgagcgccg gaaatacctg
      661 ccgtaaggta aacagcaagc gaaatcccta acccccaaat gatacaaatt tcccacagtc
      721 ccagacttgc cccggcaact ttcagggcgc acaaacaacc aatgccaaaa aagaggaaca
      781 gtccggtgcc caaaaactcg gcaacgcatt gcgccttgag tgaatcattc atagtgacac
      841 cttttttacg gagtttttct ttatcagcca actatgcgct gactgttgta tataccgagc
      901 acaactataa aaaattgctc tgcagaaaat attgccaaca tgcatgacag actcgtgtaa
      961 aaatcgtgct aacataacaa attattgtca ttaccgaatc atgcaacaca gcgttagtca
     1021 gtgttgaaag taataattcc caccaa
//
LOCUS       DAZRUF010000469         1032 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZRUF010000469 DAZRUF010000000
VERSION     DAZRUF010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.472"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..427
                     /locus_tag="ACHXOL_004397"
     CDS             <1..427
                     /locus_tag="ACHXOL_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR6811181.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
     gene            438..953
                     /gene="ogt"
                     /locus_tag="ACHXOL_004398"
     CDS             438..953
                     /gene="ogt"
                     /locus_tag="ACHXOL_004398"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR6811182.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
BASE COUNT          219 a          255 c          262 g          296 t
ORIGIN      
        1 attcatcgcc gtggggctga ccgatatact ggaaagttca gggcttagcg gcatcccggc
       61 gtttgtcggt ctggcgttgc tttcctcttt cttatgcatg tttattgcca gcggttccgc
      121 aatctggtcg attctggccc ccattttcgt accaatgttt atgctacttg gctttcaccc
      181 ggcatttgcg caaatcctct ttcgtattgc cgactcatcc gtattgcctt tagcgccggt
      241 atctcctttt gttccactgt ttcttggatt cctgcaacgc tacaaaccag acgcgaaact
      301 gggtacttac tattcgttag tcttgcccta tccgcttatc tttttggtgg tatggctgct
      361 gatgttgctg gcgtggtatc ttgttggtct gccgataggt ccggggattt acccacgttt
      421 gtcttaagag agaacggatg ctgagattac ttgaagaaaa aattgccacg ccactgggtc
      481 cactgtgggt gatttgcgat gagcaatttc gcctgcgggc ggttgaatgg gaagagtaca
      541 gcgaacgcat ggtgcagctg ctggacatcc attatcgcaa agaaggctat gagcgcattt
      601 ctgccaccaa cccaggcggt ttaagcgaca agcttcgtga ttattttgcc ggtaatctta
      661 gcattattga tacgcttccc actgccacag gggggacgcc atttcagcgc gaagtctgga
      721 aaactctacg cactatcccc tgcggacagg taatgcatta cggccaactg gctgaacaat
      781 tgggccgccc tggcgcggcg cgtgccgttg gtgcggcaaa cggatcgaat cccatcagca
      841 tcgtcgtacc ttgccatcgg gttattggcc gaaacggcac catgaccgga tatgcaggcg
      901 gagttcagcg aaaagagtgg ttattgcgcc acgaaggtta tcttttgctg taaacattaa
      961 acaatttgtg ccagcttgtt cacactttta tgtaaagtta cccttaacaa cttaagggtt
     1021 ttcaaataga ta
//
LOCUS       DAZRUF010000470         1024 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZRUF010000470 DAZRUF010000000
VERSION     DAZRUF010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.473"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..179
                     /locus_tag="ACHXOL_004399"
     CDS             <1..179
                     /locus_tag="ACHXOL_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR6811183.1"
                     /translation="QHLFESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMA
                     ETIAHHQVDIDLED"
     gene            185..>1024
                     /locus_tag="ACHXOL_004400"
     CDS             185..>1024
                     /locus_tag="ACHXOL_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR6811184.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIA"
BASE COUNT          308 a          234 c          249 g          233 t
ORIGIN      
        1 ctcagcacct gtttgaatcg ctctgcatcg accatttgca acgccacggt gccaataaac
       61 aggcaatcag ccacgcattt gatgatgatg ttgagtttca ggagcgcatg gcagaacaca
      121 tccggtacat ggctgaaacc attgcccacc accaggttga tattgatttg gaggactaaa
      181 atctatggcg ctttttcaac gagcaataaa cacacaggcc tatctaaaag caggaattat
      241 ggggtttgct ggcgacggca aaacctacac agcaagcgaa ctggctattg gtctggtaat
      301 gcttatgcgg caacggggaa ttgaagctgg taaccgcccg gtaatgtttc tggacacaga
      361 aacaggctct gactgggtta aaccccgctt tgatgcggaa aatatcgaat tatttacggc
      421 aaaaacccgc tcgtttgttg atctgcttga agccattaat gaagcggaaa gcagcggttc
      481 cgtaatgatc atcgactcca tcagtcactt ctggaccgga ttatgcgacg agtatgccag
      541 acgccgaaac cgtaaacgag gactggaatt ttcagactgg gcgtggctca aacaggagtg
      601 gcgacgcttt accgatcgat ttgttaacag tcaggcgcac attattatgt gcgggcgtgc
      661 aggatatgag tatgactttt tcgaaggtga tgatggcaaa cgccagttgg caaaaaccgg
      721 tataaaaatg aaagcagaaa cagaaaccgg ttatgaaccc tccatcctga tccagatgga
      781 gaagcaaatg aacatagaaa ccggtcaggt gtggcgcacc gccagaatac taaaagatcg
      841 ctccacccgt attgatggac aggtattttc aaacccaaca tttaaaaact ttcttcctca
      901 tatcgagttt cttaacctgg gaggtgaaca cctcggcatc gatacctcac gggataacag
      961 tgaattattt gccaacgacg gtacaccgac atggctaaaa gaaaagcgtg caaaagaaat
     1021 cgca
//
LOCUS       DAZRUF010000471         1023 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZRUF010000471 DAZRUF010000000
VERSION     DAZRUF010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.474"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHXOL_004401"
     CDS             complement(77..865)
                     /locus_tag="ACHXOL_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR6811185.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHXOL_004402"
     CDS             complement(905..>1023)
                     /locus_tag="ACHXOL_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR6811186.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZRUF010000472         1023 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZRUF010000472 DAZRUF010000000
VERSION     DAZRUF010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.475"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>1023)
                     /locus_tag="ACHXOL_004403"
     CDS             complement(<1..>1023)
                     /locus_tag="ACHXOL_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR6811187.1"
                     /translation="HLITEHKIYGRDRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDS
                     ETQSNWKDGYCRSAYLLDNKEWQEKASDLCLELIDTQDDDGYIGIYDSSLKFNCKAEN
                     GEFWAQATALRFVLGCYESTRNESLLTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHS
                     HGLTITDVFYRLYEITNNIEYLSYCLWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHG
                     VHTYEHIRALAIAASQRPQYQTALDIFLSQLKYYLTPSGAPIGDEWITGRTANATYTG
                     YEYCSVHELFSTYVMLIKLTTNIGLADDAEWLFYNASFGMQHPILHAIMYCKTDNCYE
                     ANERKHP"
BASE COUNT          302 a          215 c          191 g          315 t
ORIGIN      
        1 ggatgtttcc tttcatttgc ttcataacaa ttatcagtct tacagtacat tatcgcgtga
       61 agtatgggat gctgcatgcc aaaactagca ttatagaata gccactcagc atcatcggcc
      121 agaccaatat tagtggtcag ttttattaac ataacataag tgctaaatag ttcatgaaca
      181 gaacaatatt catagcctgt ataagtggca ttagctgtgc gtcctgtaat ccactcatca
      241 ccaatgggag cgccagatgg tgtaagataa tatttcaact gggataaaaa aatatctaat
      301 gcagtttgat actgcggccg ttggctcgct gcgatggcta atgccctaat atgctcatac
      361 gtatgtacgc catgaccttt aagaaggtaa ccagggtcta tgacattttt atattgcaga
      421 tcttgctccg acacgcatcc tgcggaataa tcttcataca gccacaagca atatgagaga
      481 tattcaatgt tattggttat ttcataaagg cggtagaaaa catctgttat tgttaatccg
      541 tggctatgtc cagcaaaacc ctgttccaca acgaaaggat gtgacgtttc tttagggtag
      601 ccagccataa tgcattgtgc ggcttgggta agagaagtta ataaactctc attacgggtg
      661 gactcataac atccaagcac aaaacgcaag gcagtagctt gtgcccagaa ctctccgttt
      721 tctgctttac agttaaattt taatgatgaa tcataaattc caatgtagcc atcatcatct
      781 tgcgtatcga taagttccaa acataaatcg ctcgcttttt cctgccattc tttattatct
      841 aacaagtagg cactgcggca atatccatcc ttccaattcg attgagtttc actatcccac
      901 caataatatt gcatcatgtt atcatcggga ttattctgtt ttttaataac gccatcttca
      961 gcagatacag tgcgtggagt tatacgatct cttccatata ttttatgctc cgtaataaga
     1021 tgg
//
LOCUS       DAZRUF010000473         1017 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZRUF010000473 DAZRUF010000000
VERSION     DAZRUF010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.476"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(177..752)
                     /locus_tag="ACHXOL_004404"
     CDS             complement(177..752)
                     /locus_tag="ACHXOL_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR6811188.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLINHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          274 a          223 c          214 g          306 t
ORIGIN      
        1 gtgcagtctg aatagataac ttagcgttat gtctagtcgt ttccctctcc attcctaaaa
       61 agtgctgtat actggcctat acaacgctat aaagacctct acatagcgcc ttgatagcta
      121 tgtcacaggt acaaaaaagc cccctataaa ggaggctgtg tagtctatta agggtcttac
      181 agggtcttgg tccagtgtct gctaacagtc cttacagaga ggttaagaac cttagctacc
      241 tgagactgag atagtcccct ttccttgtac tctaagaccg ctctagttgt gtcactcgct
      301 ataggtcttc caagtacctt accttgaact ctagcacgct ctagaccctg tttagtgcgc
      361 tctctgatac ggtttctttc aaactctgca aaggtactaa atagctgtag aaccaactta
      421 ccctctgagc ttgaaaggtc tttaacgggt aaatctaaac agatgagctt cacaccctta
      481 tcattaagaa gggtcactgt attctgtagg tcgatgttat cccttcccag acggtcaagt
      541 ttaagaacaa caagggtgtc accttcttct agcttatggt tgatcagatt cgcaaatccg
      601 tccctatcca tagctgacac tgaaccagag acaacctcct ctataactcg tgacttagga
      661 atatcatagc cagccttctt aatggcttga atctggttct ctgtggtgga agtctctact
      721 gatacacggc agtaagcaaa ggttcgtgac atagatatcc ctctgggtag tcattaaacg
      781 gttagtttgt cttgtctata gacattaaag caaatggtag gtttgttgtc tatagctata
      841 gtgactcttc tatgtagata ttaaacggac gtttcttgtc gtgggtcaca tagatacttt
      901 tagggcttct gtcaagtgat attcgtagat tttgcaaggg ggaggtgtaa ggctgaaaca
      961 gggctactaa gaacacatac atctaaggct ggaaagtacc tgagcagata ttcttca
//
LOCUS       DAZRUF010000474         1014 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZRUF010000474 DAZRUF010000000
VERSION     DAZRUF010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.477"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(242..913)
                     /gene="queE"
                     /locus_tag="ACHXOL_004405"
     CDS             complement(242..913)
                     /gene="queE"
                     /locus_tag="ACHXOL_004405"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR6811189.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          284 a          242 c          213 g          275 t
ORIGIN      
        1 gtacttaatt cattgattaa aaaggtaaat atttaaaata actctataca aattaaaatc
       61 taccaaactt taccgcaata attttcactc cagcgaaaaa ttaatgccac agaatttgta
      121 gagaatgcta atgattagcc ctggatgggt aaaatggagg agttttcaga ggcgacaaac
      181 aatataaatg agtagaagtc tttacaggtt ccacccattt ataacgctta taaatgttta
      241 atcaggcaat atttagatat ttatgtgttt gcatcgacaa acgccaatta cgcgcaatgc
      301 aggtttcaat gcacaaacgt gtggcatcat ccttttggct aatcggctgc agtgcaatga
      361 ctcgcggttt atcatcggtc agcgttgcca atagttcatc cagtgcttca atatcgcgta
      421 cgcgccccac tggatgcttg atttcgttgg ctcgctccag tgcctgtgac aacacgtcat
      481 agccgccgcg catgttcagc tttggcgata cggtaaccca ggtattcggt gtgcagcgta
      541 cctcatgagt accactggtt tcgatctggc agctaaaacc gttcttttcg agcagatcag
      601 tcagtggtag caaatcatga atgcaaggct caccacccgt aatcaccaca tgccgcgcgg
      661 tgtatccctg gcgactaatg acagccaaca agtcttcact gctcgccgcc ccccacttat
      721 cactctcttt ggtcttcgcc agaatgctga aaagggagac ttcccgatcc tcaagctttt
      781 cccaggtgtg tttggtgtcg caccaggcac agccaaccgg gcatccctgt aaacgaataa
      841 aaatggcggg gacgccggta aagtaaccct caccttgcag ggtctggaac atctcgttaa
      901 tcgggtactg catagcattc tctgtgaagt ggataattgt taattattgc agatcctgcc
      961 acaacaatca tgtcttatta acattctgtt acaggcagat ttaaagaaaa atgc
//
LOCUS       DAZRUF010000475         1012 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZRUF010000475 DAZRUF010000000
VERSION     DAZRUF010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.478"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(120..974)
                     /gene="rclR"
                     /locus_tag="ACHXOL_004406"
     CDS             complement(120..974)
                     /gene="rclR"
                     /locus_tag="ACHXOL_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR6811190.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT          276 a          267 c          231 g          238 t
ORIGIN      
        1 ctagagtcca tcagaaaaaa atatataaag cccaaaccac cgttggaaat agaaatagtt
       61 gaaaggctca caactcccgg aatgcggatt atctttgccc tgatttctgg cggttttatt
      121 cagggtgcaa gctgtctgac cctttcccga tattctcccg gagtacaacc aaactcgcgg
      181 acaaacgcct tgtgaaaaga tgattcactg gcatagccta ctgactcagc gatcaccaca
      241 acagggagca tttcccggga aaacatctgg gccgctattt gtagacgcaa ctttgttaat
      301 acagccagcg gcgtggttcc ggaaacatca cggaaaagct gggcaaaact tgcccgagac
      361 atgtgggcga tgctggccag cgattcgacg gtccaggcgt gtcctggcat ttccagcatt
      421 tgctgtatta ccgcaccaag acgtggatgc agaagcaaac tgagaatgtt tttctctgta
      481 ttaacctgtg caatccactc acgcaccgcg agggtaaaga atgtagcgca gatctggcta
      541 cacagtgcat ccactcccgg catcgccgat ctggattcct gttgtaaaaa cgggatcgcc
      601 tccctcagcc agttatattc cacgctgtgg ttaaccggtg ctaaaaacag cgtttccggc
      661 aaagacgtta aaaaataacg cgccgaatgt tgcaaccgaa gagtgccgca gacaatacag
      721 gtcgattcat tatcgacatg actcaggcga tgagctgaat tttgtggtag caggaccaca
      781 tttcccgggc gtaatgtaaa aatctctccc gtcggcattt ccagcttcgc cgctccttgc
      841 gttaacgcat gccaacgaat aaccgataat tccccggcac catgttgaag ctgccagtca
      901 cttcctaaca cgcaattctt atcgatcgtt ccttgtggag cgttaagcat caacaaacgg
      961 ctaagggcat ccatatcaga ctcctgagtg gtttttgatt acaaacacca ac
//
LOCUS       DAZRUF010000476         1009 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZRUF010000476 DAZRUF010000000
VERSION     DAZRUF010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.479"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            59..>1009
                     /gene="phoE"
                     /locus_tag="ACHXOL_004407"
     CDS             59..>1009
                     /gene="phoE"
                     /locus_tag="ACHXOL_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR6811191.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKN"
BASE COUNT          296 a          207 c          258 g          248 t
ORIGIN      
        1 gttttcaacg gggctctccc gctgaatatt cgcgcgttaa ttaaaatcag gaatgaaaat
       61 gaaaaagagc actctggcat tagtggtgat gggcattgtg gcatctgcat ctgtacaggc
      121 tgcagaaata tataataaag acggtaataa actggatgtc tatggcaaag ttaaagccat
      181 gcattatatg agtgataacg ccagtaaaga tggcgaccag agttatatcc gttttggttt
      241 taaaggtgaa acacaaatta acgatcaact gactggttat ggtcggtggg aagcggagtt
      301 tgccggaaat aaagcggaga gtgatactgc acagcagaaa acgcgtctcg cttttgccgg
      361 gttgaaatat aaagatttgg gttctttcga ttatggtcgt aacctgggcg cgttgtatga
      421 cgtggaagcc tggaccgata tgttcccgga atttggtggc gattcctcgg cgcagaccga
      481 caactttatg accaaacgcg ccagcggtct ggcgacgtat cggaacaccg acttcttcgg
      541 cgttatcgat ggcctgaact taaccctgca atatcaaggg aaaaacgaaa accgcgacgt
      601 taaaaagcaa aacggcgatg gcttcggcac gtcattgaca tatgactttg gcggcagcga
      661 tttcgccatt agtggggcct ataccaactc agatcgcacc aacgagcaga acctgcaaag
      721 ccgtggcaca ggcaagcgtg cagaagcatg ggcaacaggt ctgaaatacg atgccaataa
      781 tatttatctg gcaactttct attctgaaac acgcaaaatg acgccaataa ctggcggctt
      841 tgccaataag acacagaact ttgaagcggt cgctcaatac cagtttgact ttggtctgcg
      901 tccatcgctg ggttatgtct tatcgaaagg gaaagatatt gaaggtatcg gtgatgaaga
      961 tctggtcaat tatatcgacg tcggtgctac gtattatttc aacaaaaat
//
LOCUS       DAZRUF010000477         1003 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZRUF010000477 DAZRUF010000000
VERSION     DAZRUF010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.480"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>1003
                     /locus_tag="ACHXOL_004408"
     CDS             <1..>1003
                     /locus_tag="ACHXOL_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFR6811192.1"
                     /translation="KLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSGAYQKLTIG
                     SDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGITNKASSYG
                     VPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIATRNQLLKSL
                     YYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDNTLITFSNE
                     EIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRTFATALANI
                     EKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECDLNLVSGES
                     "
BASE COUNT          311 a          189 c          211 g          292 t
ORIGIN      
        1 caaactgtcg aataaaatag gtcagcgtgt ttatactgtt gtctctgaaa tgtcgattga
       61 agcatggata acaaaagaac cgcagccttt tgtttatcgc cgtagcggcg cttaccagaa
      121 actcaccatt ggctcagact ggggtcaact gtttgactgt gcctggatgc gagtttatgg
      181 taaacgtcca gcagataaat ttagacacaa actcgtcgct ctcgttgata tcggtggcga
      241 aggtcttatt gtggataaga atggttcacc catctgcggc atcacgaata aagcatcatc
      301 ttatggcgta ccgccagata aaccaggtaa atgggtggtt gacctttctc tcgttagtga
      361 aaatgacgag gttgagtttt ggattgatgc tgcctgcaac gatttatttg gctatgttac
      421 taatggaggg attattactg atgttcatat cgcgacacgt aatcaactac tgaaatccct
      481 ttattatgat attgaggtgc ttttcgactg gattaatgat ggtcagaagt ttgaatccat
      541 acatcctaaa ggtatcaccc cagaacaatt tattgccaaa agaagtgagc gtactgacga
      601 aatcataagt actctcgaat atattgataa tacgttaatt acctttagca atgaagaaat
      661 aattaaatgt caaattgcaa tccaatccat aatcaataaa aacaataatc cttcagagtt
      721 caggattatg gctactggtc atgcccattt agatatagcg tggatgtggc ctttaagaga
      781 aggacgcaga aaagcaataa gaacatttgc caccgccctt gcaaacattg aaaaatatcc
      841 tgattacatt tttggcgcta gtcagtacca actttttcac tggataaaaa aagattatcc
      901 ctatttcttc gaaaagttaa aaaaacaaat cgccgcaggc cgctttgagt tacaaggttg
      961 cttctgggtc gaatgcgatt taaatctggt aagtggtgaa tcg
//
LOCUS       DAZRUF010000478         1003 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZRUF010000478 DAZRUF010000000
VERSION     DAZRUF010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.481"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            191..451
                     /locus_tag="ACHXOL_004409"
     CDS             191..451
                     /locus_tag="ACHXOL_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFR6811193.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEDIPWHDLPASHRGNNQMSLPLPQN"
     gene            642..782
                     /locus_tag="ACHXOL_004410"
     CDS             642..782
                     /locus_tag="ACHXOL_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR6811194.1"
                     /translation="MSQKSLTTITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT          278 a          254 c          240 g          231 t
ORIGIN      
        1 tcctgatctt ttcccaaaat agatgacagg ataaaaaaca ttccaatctt ttacaggctt
       61 tggataaatc gtatcaaagc ctatttcttc aagagttaaa tcaccgcttt tcgctcgaaa
      121 cggactggct gttggagtat gagccagaac aacttagaat agcccctgca ctacgttaag
      181 ggaggtcgct atgttccgtt gtccgctttg tggcgcatct gcccgtatcc gcaccagtcg
      241 tccggaaaat gattcaaaca ccgtgcggca aaagtattac cagtgtaaca atctggaatg
      301 cggcgtatgc ttctcaacac tggaagcttt ccataaattc acatcaaaac acgcctccgg
      361 cgttcactct tcagaagata tcccgtggca tgatctgcca gcttcacaca ggggaaacaa
      421 tcagatgagc ttgcctttac ctcagaatta acaggcagaa ttgccggagt aacaaaaaag
      481 cgatagatta cgcgcgggtg cctttcggct gatggtcgga gggaataccc gaaggccaga
      541 tgtggaaagg ccccggaaaa catctctgtt taaccgaggc cctaaccgca ttaccttgac
      601 aagtgaaagg ttagcgcctc tccggaaaag gagcaagtgc tatgtcgcaa aaatcgctta
      661 cgaccatcac gttctgcgtg acggcaatcc tcatcatctg gatgttgcac ggttcgctgt
      721 gtgaaatacg gatgagcttc tggggagcgg agtttgcggc gttcttacag tgtaagcagt
      781 aaggaaaccg cgacggggga gcaatccccc gtcaatcggt tgccagggta aggccggata
      841 aggcacccta tctcacagat atgaacaaca aacccgcagc gtaaaaactg cgggttttct
      901 ttttggtgcc ctcaccgact tgaaacagtg ataagactat aagacaaagc ccgcaatgta
      961 gcgggcttac tcccctcacc ctccactgaa tgtggacaca cag
//
LOCUS       DAZRUF010000479          992 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZRUF010000479 DAZRUF010000000
VERSION     DAZRUF010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.482"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            63..749
                     /locus_tag="ACHXOL_004411"
     CDS             63..749
                     /locus_tag="ACHXOL_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR6811195.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(840..976)
                     /locus_tag="ACHXOL_004412"
     ncRNA           complement(840..976)
                     /ncRNA_class="other"
                     /locus_tag="ACHXOL_004412"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT          260 a          217 c          245 g          270 t
ORIGIN      
        1 cacgctgcag cgagtaacat tggtaatgaa atataatcaa catggatagg tgaaaattaa
       61 ttatgagctt tatggctttt gtaacttgtt tgacaattgc atttatcgcc ggttttgctg
      121 ttgccagaaa attgacactc cgaaaagttg cctcatcggt aatcatcgct aaatcgcaac
      181 ccgtcccttc atctgcaact tcccctgccg ctaaggcgtc gacaaggagc tatataccgc
      241 agaaaaccta tcgtgactct cgttggcaag agcgagatga gttatttcag ggaatggttg
      301 ccgaaggtat tttattggaa agaaaagagt ggttaatcac gcgttgtgaa aaaatgtatt
      361 tcgataatct ggaaaaatgg tttggacatt attgtcatat ccattgccag gtctctctgg
      421 ggcgcttgat caactttcca gaacaacctg agtttagcga agaagaaaga aagcgtttct
      481 ttgcaattta taatggcatg gcaatggatt atgttctggt ttcaaagaag aacaataaaa
      541 tagtctgtgt tattgagctg gatgacccga gtcatgaaag ggcggatcgc attattcgtg
      601 accgttggtt ggatacaatt atgactcttg cacgggtgcc ttttgtacga gcttctgtca
      661 ctaaattgga ggaaattcct ccggtctggg agactcgtga caaaattcgc ccattagtcg
      721 cggcgacaaa gagtgaaccg gtgcattaac taagtatccc ggtcggggct tctcatcccc
      781 ccggtgtgtg caatatacga aaaaaagccc gtaccttcgt acgagctctt ctttaaagat
      841 ggcggtgagg gggggattga ctcgcttcgc tcgccctgcg ggcagcccgc tcactgcgtt
      901 cacggtctgt ccaactggct gtcgccagtt gtcgaacccc ggtcggggct tctcatcccc
      961 ccggtgtgtg caatatacga aaaaaaagcc cg
//
LOCUS       DAZRUF010000480          984 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZRUF010000480 DAZRUF010000000
VERSION     DAZRUF010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.483"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            229..>984
                     /gene="gspA"
                     /locus_tag="ACHXOL_004413"
     CDS             229..>984
                     /gene="gspA"
                     /locus_tag="ACHXOL_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR6811196.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITC"
BASE COUNT          280 a          199 c          236 g          269 t
ORIGIN      
        1 catctttatt atgattcgcc agatggaagg ggaaacgtag tgtgggcacg atgtatgttc
       61 taataaaata gattgggtgg gttgtatatt gacgttagta taaatagtat taatgccgct
      121 tgcatgaatc aagcaaaatt tatttatatt agtaatatgt atttaattaa taaatacata
      181 cattattttt atgattaagg ataatcaata attaaaacag gaagttctat gtctacgaga
      241 agagaagtta ttctttcctg gttgcgtgag aaacgtcaga cctggcgtct gtgctatttg
      301 ttgggtgagg ctggaagtgg aaaaacctgg ctggcgcagc aactgcaaaa agataaacat
      361 cgccgtgtga ttactttaag cctcgttgtt tcctggcaag gtaaggccgc atggatcgtt
      421 accgacgata acgcggctga acagggctgc cgtgacagcg cctggacgcg agatgagatg
      481 gcggggcaat tactgcatgc gcttcatcga actgatagtc gctgccctct tattattatt
      541 gaaaatgctc acctgaatca ccgccgcata cttgatgatc tccaacgcgc gatatctctt
      601 attcctgacg gtcaattttt gctgatcggc agacccgatc gaaaagtcga acgtgatttt
      661 aaaaaacagg gcattgaact tgtctcgaca ggacgtctga cggagcacga acttaaggca
      721 agcatccttg aaggtcagaa tattgaccaa cccgatctcc ttttaacctc cagagttctg
      781 aaacggatag ctttattatg tcggggcgat cgcagaaagc tggcgcttgc cggcgaaaca
      841 attcgcttat tacagcaagc cgagcagacc agcgttttca ctgcaaaaca atggcgaatg
      901 atttaccgca tattgggtga taaccgcccc cggaaaatgc agcttgccgt ggtaatgagt
      961 ggaaccatta tcgctattac ctgt
//
LOCUS       DAZRUF010000481          984 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZRUF010000481 DAZRUF010000000
VERSION     DAZRUF010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.484"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            25..480
                     /gene="csgB"
                     /locus_tag="ACHXOL_004414"
     CDS             25..480
                     /gene="csgB"
                     /locus_tag="ACHXOL_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR6811197.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            521..973
                     /gene="csgA"
                     /locus_tag="ACHXOL_004415"
     CDS             521..973
                     /gene="csgA"
                     /locus_tag="ACHXOL_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR6811198.1"
                     /translation="MKLLKVAAIAAVVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          276 a          204 c          249 g          255 t
ORIGIN      
        1 tacgaccagg tccagggtga caacatgaaa aacaaattgt tatttatgat gttaacaata
       61 ctgggtgcgc ctgggattgc agccgcagca ggttatgatt tagctaattc agaatataac
      121 ttcgcggtaa atgaattgag taagtcttca tttaatcagg cagccataat tggtcaagct
      181 gggactaata atagtactca gttacggcag ggaggctcaa aacttttggc ggttgttgcg
      241 caagaaggta gtagcaaccg ggcaaagatt gaccagacag gagattataa ccttgcatat
      301 attgatcagg cgggcagtgc caacgatgcc agtatttcgc aaggtgctta tggtaatact
      361 gcgatgatta tccagaaagg ttctggtaat aaagcaaata ttacacagta tggtactcaa
      421 aaaacggcaa ttgtagtgca gagacagtcg caaatggcta ttcgcgtgac acaacgttaa
      481 tttccattcg acttttaaat caatccgatg ggggttttac atgaaacttt taaaagtagc
      541 agcaattgca gcagtcgtat tctccggtag cgctctggca ggtgttgttc ctcagtacgg
      601 cggcggtaac catggtggtg gcggtaataa tagcggccca aattctgagc tgaacattta
      661 ccagtacggt ggcggtaact ctgcacttgc tctgcaaact gatgcccgta actctgactt
      721 gactattacc cagcatggcg gcggtaatgg tgcagatgtt ggtcagggct cggatgacag
      781 ctcaatcgat ctgacccaac gtggcttcgg taacagcgct actcttgatc agtggaacgg
      841 caaaaattct gaaatgacgg ttaaacagtt cggtggtggc aacggtgctg cagttgacca
      901 gactgcatct aactcctccg tcaacgtgac tcaggttggc tttggtaaca acgcgaccgc
      961 tcatcagtac taatacatca tttg
//
LOCUS       DAZRUF010000482          983 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZRUF010000482 DAZRUF010000000
VERSION     DAZRUF010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.485"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            38..823
                     /locus_tag="ACHXOL_004416"
     CDS             38..823
                     /locus_tag="ACHXOL_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811199.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          322 a          180 c          216 g          265 t
ORIGIN      
        1 attaccatga ttatgacagg cgagggagtg tccgattatg aatttaagaa gggtttgttg
       61 tatatgtgta ttattctcgc tgcttgcagg atgtgcctct gaatcttcta ttgatgaaaa
      121 gaagaaaaaa gctcaagtca cacaaagtaa tattaataaa aacactcccc agcaactgac
      181 agacaaagat ttattcggta atgaaaccac tctggccgta tccgaagagg atattcaagc
      241 tgcgcttgat ggagatgagt tccgcgttcc ccttaattct cctgtaatac ttgttcaatc
      301 cggcaaccgc gcaccggaaa ccattatgca ggaggagatg cgaaaatatt atactgtttc
      361 cacattctct ggtatcccgg acaggcaaaa gcctctgact tgtaacaaaa acaaagataa
      421 aaatgagaac gaggatgttg ccagtgctga gaatatgaac tggatgcagg cactgcgttt
      481 tgtggctgca aaagggcatc agaaagcgat tattgttttc caggatacgt tgcagacagg
      541 aaaatatgac tctggattga aatcaatagt ttggtcagat tataaaaatc aaaaattgac
      601 tgacacgatg tcactgcgct atctggtacg attcacgctg gtggatgtag ctacaggtga
      661 gtgggctgcc tggtcgccgg tgaattatga atataaagtg cttccaccat tgcccgacaa
      721 gaatgaagcc agtacgactg atatgacaga gcagcaaatc atgcaactta agcaaaagac
      781 ctataaagcg atggtaaaag atttggttaa ccgctatcaa taataaatta tatctgccgc
      841 caggaatttc tggcggaaat aatacaaaat tctttgcata agaaattttt cttgacaaag
      901 acagtgcaag aaaggacatg gaatccggag ttaaaaactg tttgttttag aatgtttaat
      961 tatttaactg ctgaatcttc ctt
//
LOCUS       DAZRUF010000483          979 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZRUF010000483 DAZRUF010000000
VERSION     DAZRUF010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.486"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..635
                     /locus_tag="ACHXOL_004417"
     CDS             <1..635
                     /locus_tag="ACHXOL_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR6811200.1"
                     /translation="HHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHDTLLPRFTDDI
                     SRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRHIYSNNHCHEI
                     RILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCADTLLPLTIINR
                     GGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQRLS"
BASE COUNT          285 a          211 c          193 g          290 t
ORIGIN      
        1 agcatcatgg gggttctgat tactggagaa ctccattttt gttatttaag aacttacacc
       61 gtgaatttat tttcagtcta gatggtgctg caactgagca tgacacgctg ttaccacgat
      121 tcaccgatga tataagccgt caatcatggg ttagtgaaaa ggtcttctgt aacccgccgt
      181 acagcgatat tccgtcgttc ctcttaaaag cgtctgaagc ggatttagta gctttcctga
      241 taccgcatag agccaacacc agctactggc ttaggcacat ttacagcaat aaccactgtc
      301 atgaaatccg tattcttcat cgtgcagtta aataccttcc accagcaggg cataacaggc
      361 ttactattag gtcaccgttt ccaagtgcag tcgttgtttt taaaaaagaa ccacgtaagc
      421 atgaaattac acagatggtt tgctgtgctg atacattgct accgttaacc atcattaacc
      481 gtggtggact aagaggaaga cccaccattt acccacctga gacgctcgac agcttcataa
      541 aactatacag gcaaggaaaa cccattaagt acattgcaga tgctctcaga atgcctctga
      601 gcacgtcata caggattgct caaaggttga gctaaaattc tcataaatat ggtcatttac
      661 aagacaggtg attcagatgt taagaagtat ccctgtgaac aataaatgac caatcagtaa
      721 tcattctgtg aaataattgt tgcaatttat aatcacatct gttatagttt ctatatgggc
      781 tgataaaaaa gttcatgtct tgaagtggta acacttgggg aggtgtcaga tggttggcgt
      841 atattaacct ctcattcagg gtatacctca gcgttttaat catcgtactg tttcaggcat
      901 ggacttcttt atcagtccat caatccccca gatattttaa tcacacaggc taatcggttt
      961 tatccggttg gccttttca
//
LOCUS       DAZRUF010000484          974 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZRUF010000484 DAZRUF010000000
VERSION     DAZRUF010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.487"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>974
                     /gene="tssI"
                     /locus_tag="ACHXOL_004418"
     CDS             <1..>974
                     /gene="tssI"
                     /locus_tag="ACHXOL_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR6811201.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQ"
BASE COUNT          250 a          261 c          298 g          165 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg
      961 ttaccaataa ccag
//
LOCUS       DAZRUF010000485          971 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZRUF010000485 DAZRUF010000000
VERSION     DAZRUF010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.488"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            236..>971
                     /locus_tag="ACHXOL_004419"
     CDS             236..>971
                     /locus_tag="ACHXOL_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR6811202.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPVFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLG"
BASE COUNT          330 a          202 c          171 g          268 t
ORIGIN      
        1 catcacccat tatgggaaat aaataaaagg taattcatgg atggttgaac tatatccctg
       61 cttttcaacc atcctaaaaa agcatgaatt gctcgctcta ccctaagtgg ctgtgctatt
      121 ttagaaaaac ttaatcctga gttaagcaaa aaaacataat caataaaaat acatacgcaa
      181 aaaacattga ttaagtgaat ataccatgga agaaaaatat aaaccggagt agtgtatggc
      241 caatctctac gacttgaaaa aattcgactt aaatcttctt gtcatatttg agtgtattta
      301 ccagcattta agcattagca aagctgctga atcactatat atcaccccat cagccgtgag
      361 ccagtcgtta caacgcttac gtgcgcaatt taatgaccct gtattcattc gctcaggaaa
      421 aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac catcttgaga aaaacctcaa
      481 aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa actttattat
      541 ctacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct gcctacggca
      601 agatgcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga atgctgaaga
      661 actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga tcagccgttc
      721 cgtcatttgc atgccattgc atacaattcg caatactcta atttgtagta ataagcatcc
      781 gcgaattact gacaatagca cttatgaaca aattatggca gaagagttca cccaattaat
      841 atccaaatca gcaggagttg atgatattca aatggaaata gacgagaagt ttatgaatcg
      901 caaaatttca tttcgtggtt cctctttgct gacaataatt aatagcattg cagttacaga
      961 tttactggga a
//
LOCUS       DAZRUF010000486          958 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZRUF010000486 DAZRUF010000000
VERSION     DAZRUF010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.489"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            6..958
                     /locus_tag="ACHXOL_004420"
                     /pseudo
     CDS             6..958
                     /locus_tag="ACHXOL_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT          236 a          279 c          293 g          150 t
ORIGIN      
        1 cgtagggtga agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca
       61 cgcagagtca gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga
      121 caacagaata cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg
      181 gcagggcatc ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg
      241 acgggctgga aatgcgccgc aaatatgatg aatccggccg tctgattcag gaaaccgccc
      301 ctgacggcga tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa
      361 cggaagatgc caccggcagc cggaaaacca tgacatggag ccgttacggt cagttgctga
      421 ccgtcactga ctgttccggt tatgtaaccc gttatgacca tgaccgtttt ggtcaggtga
      481 cggcggtgca ccgcgaggaa gggctgagcc agtaccgcgc atacgacagc cgtggacagt
      541 taattgcggt gaaagacacg cagggccatg aaatgcggta tgaatacaac gccgccggtg
      601 acctgaccgc cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg
      661 gaaaagccat ctgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg
      721 gacgggtcat ccgtctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac
      781 tcgaccggct gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc
      841 tgaccggcaa acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg
      901 aagcagaccg cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg tggcggta
//
LOCUS       DAZRUF010000487          939 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZRUF010000487 DAZRUF010000000
VERSION     DAZRUF010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.490"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..832
                     /gene="yeeO"
                     /locus_tag="ACHXOL_004421"
     CDS             <1..832
                     /gene="yeeO"
                     /locus_tag="ACHXOL_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR6811203.1"
                     /translation="GLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKP
                     LNFSIIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLP
                     GSALGSASTIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYT
                     QDPQVKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVG
                     YVLGIMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE
                     "
BASE COUNT          188 a          189 c          271 g          291 t
ORIGIN      
        1 gggactggga tttgtcgggg cagggctggg tttaaccatt tctcgttata ttggcgcagt
       61 tgcaattttg tgggtgctgg cgattggttt taatcctgcg ctaaggattt cgttaaagag
      121 ctattttaaa ccgctgaatt ttagcattat ctgggaagtc atggggattg gtattcccgc
      181 gagtgtcgaa tcagtgttat ttaccagtgg tcggttatta acccaaatgt tcgttgccgg
      241 gatggggacc agtgttattg ccggaaattt tatcgcgttt tcaattgcgg ctcttatcaa
      301 cttacccgga agtgcgctcg gctctgcttc tacgatcatt acaggccgaa ggttgggggt
      361 agggcagata gcgcaagcag agattcagtt gcggcatgtg ttctggcttt ccactcttgg
      421 attaacggcc atcgcctggc taacggctcc ctttgccggg gttatggcat cgttttacac
      481 ccaggatcca caggttaaac atgtcgttgt gattctgatt tggctaaatg ctttatttat
      541 gcctatttgg tccgcctcat gggtgctacc cgctggattt aaaggtgctc gcgatgcccg
      601 ttacgccatg tgggtttcta tgttgagcat gtggggttgc cgggttgtcg tcggttatgt
      661 gctgggcatc atgcttggct ggggtgtggt tggtgtctgg atgggaatgt ttgccgactg
      721 ggctgtgcgg gccgtgctgt tttactggcg aatggttact ggacgttggc tgtggaaata
      781 ccctcgatcc gaaccgcaaa agtgtgaaaa aaagccagtt gtgtcggaat aaacgacaaa
      841 atgcagatta tttcagcaaa cgatttcaaa tttaaaaaac aggctttgac attgtgggtg
      901 ggcatcgcta atattcgcct cgttctcacg attcctctg
//
LOCUS       DAZRUF010000488          935 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZRUF010000488 DAZRUF010000000
VERSION     DAZRUF010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.491"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(197..>935)
                     /locus_tag="ACHXOL_004422"
     CDS             complement(197..>935)
                     /locus_tag="ACHXOL_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR6811204.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          259 t
ORIGIN      
        1 ttgcttctcc gataaaatgt cagagaagca tgattacgtg gttattgtct gttgatgact
       61 tgttgaccga gccgtcatca ttctcaatgc attagttaaa tcatttgtga tttttaagga
      121 ttatcctgtc tatgattttc tacttagaag gatatggcac taactatgca tcttatagtt
      181 tactgctggc atctatttaa aatagcaatt gagtcaaata gcccatcaag atagccatac
      241 ctaatataac cgtcaggaat gcagctatca tcggcattct gaacattgat ttaagtaata
      301 tcacttccgt caggcttgcg ccggcactgc cgatgattaa tgccattaat gctcccagac
      361 ccattccttt tgtcatcaga acagatgcca gagggataac cgcctcagcc cggatataca
      421 gcggaatacc gacaacggcg cttaatggga tggcgaaggg attatctgct cctgcatgag
      481 cggcaatcca cgctgaggga ataaagccat aaataaaaga tcctattaat actcctaaca
      541 aaagatatgg cagtacatct ttaaactgtt gcaacgcatc tttggtggct agttttatgg
      601 gattaatact tactacagct ttggtactgc aacaggtaac tacggggttc tcaatggctt
      661 tagccgctgg actacaacaa ctcactttta tgggcgtata tgtcgttccc ggcgaagttt
      721 ttgctggagc acaacaattt gctgatgagc ttttactggc aatgatatga cgttcaaacc
      781 ccagagaatc caggataata ctggcaagaa cggagacgcc ggctgcgata atcgcgtaca
      841 acaaggtgac tttccaacca aaggtcatcc acattagtcc gacgataatg ggattaagta
      901 atggtgaaac aaataaaaaa gtgagagtcg gacca
//
LOCUS       DAZRUF010000489          930 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZRUF010000489 DAZRUF010000000
VERSION     DAZRUF010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.492"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..222
                     /locus_tag="ACHXOL_004423"
     CDS             <1..222
                     /locus_tag="ACHXOL_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFR6811205.1"
                     /translation="EVRVVISEFVADGTILMGDFSNVILANWEGQEVDYDDTSYRSSN
                     TIVYRVWDYSDIALAHSEAFVNIVIGKAE"
     gene            236..559
                     /locus_tag="ACHXOL_004424"
     CDS             236..559
                     /locus_tag="ACHXOL_004424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811206.1"
                     /translation="MRVFKDTQCDAFLNAFGQSIQTCTGSTFTGIVEVLPVSIEAAGG
                     FIESVETYLTIRKDDLITANVAIGTVLIIDGVNQTIYNIEDDLSGMVNCYFRTSAGAS
                     FAEDY"
     gene            561..>930
                     /locus_tag="ACHXOL_004425"
     CDS             561..>930
                     /locus_tag="ACHXOL_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811207.1"
                     /translation="MMLVQKVRNTMKQLINATKNLTVSRDVDVFEQIAFDYSLTSLSF
                     SNERRAGNFAIQYLISPKPESGNTAPSITYDQIISTFDKEKTSAFSDAGLIMLSCSYE
                     QSDIVTDPITGSVSLSFTINI"
BASE COUNT          289 a          164 c          188 g          289 t
ORIGIN      
        1 gaagtacgtg tagtgatttc tgaattcgta gcagatggta ctattctaat gggcgatttc
       61 tccaatgtaa ttctcgctaa ttgggaaggg caggaagtag attatgacga tacatcctat
      121 cgttcaagta acactatcgt atatcgcgta tgggattact cagatatcgc acttgcacac
      181 tctgaagctt tcgtaaatat cgttattggt aaagcagaat aataaggatt agataatgag
      241 agtatttaaa gatacgcagt gtgatgcgtt tctaaatgct ttcggtcagt ctatccaaac
      301 ttgtacggga agtactttta caggaatagt agaagtactt cccgtttcta ttgaggcggc
      361 aggtggattt atcgaaagcg tggaaacata cctaacaatc agaaaggacg atctaataac
      421 cgcaaacgtg gcaattggta cagtactaat cattgatggt gtaaaccaga caatctataa
      481 cattgaagat gatctatcag gtatggttaa ttgctatttc cgtacttctg ctggtgcttc
      541 atttgcagag gactactaat atgatgttag tacaaaaagt acggaatacg atgaagcagc
      601 ttatcaatgc tactaagaat ctgacagtat cacgtgatgt tgatgtattt gaacaaattg
      661 cttttgatta ctcattaacg agtttaagtt tcagcaatga acgccgggca ggtaactttg
      721 caattcagta tctgatttcc cctaaaccag aatcaggtaa tacagcccca tccatcactt
      781 atgatcagat tattagtact tttgataaag agaaaacttc agcattcagt gatgcaggtt
      841 taataatgct ttcgtgttct tatgaacaat ccgatattgt cacagatcct ataactggct
      901 cggtttccct atcctttacg ataaatattc
//
LOCUS       DAZRUF010000490          926 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZRUF010000490 DAZRUF010000000
VERSION     DAZRUF010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.493"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..906)
                     /locus_tag="ACHXOL_004426"
     CDS             complement(<1..906)
                     /locus_tag="ACHXOL_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR6811208.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          258 a          221 c          190 g          257 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat catttt
//
LOCUS       DAZRUF010000491          921 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZRUF010000491 DAZRUF010000000
VERSION     DAZRUF010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.494"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            2..799
                     /gene="gadX"
                     /locus_tag="ACHXOL_004427"
     CDS             2..799
                     /gene="gadX"
                     /locus_tag="ACHXOL_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR6811209.1"
                     /translation="MAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVL
                     VSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEM
                     LRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELL
                     MSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYV
                     FRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          265 a          178 c          209 g          269 t
ORIGIN      
        1 aattgcgtac gcaagacata aatatattct caccatggtt aatggtgaat atcgctattt
       61 taatggcggt gacctggttt ttgcggatgc aagccaaatt cgagtagata agtgtgttga
      121 aaattttgta ttagtatcaa gggatacgct ttcattattt ctgccgatgc tcaaggagga
      181 ggcattaaat cttcatgcac ataaaaaaat ttcttcatta ctcgttcatc actgtagcag
      241 agatattcct gtttttcagg aagttgcgca actatcgcag aataagaatc ttcgctatgc
      301 agaaatgcta cgtaaaagag cattaatctt tgcgttgtta tctgtttttc ttgaggatga
      361 gcactttata ccgctgcttt tgaacgtttt gcaaccgaac atgcgaacac gagtttgtac
      421 ggttatcaat aataatatcg cccatgagtg gacactagcc cgaatcgcca gcgagctgtt
      481 gatgagtcca agcctgttaa agaaaaaatt gcgcgaagaa gagacatcat attcacagtt
      541 gcttactgag tgtagaatgc aacgtgcttt gcaacttatt gttatacatg gcttttcaat
      601 taagcgagtc gcagtgtcct gtggatatca cagcgtgtcg tatttcattt acgtctttcg
      661 aaattattat gggatgacgc ccacagagta tcaggagcga tcggcgcagg gattgccgaa
      721 ccgtgactcg gcggcaagta ttgttgcgca aggaaatttt tacggcactg accgttctgc
      781 ggaaggaata agattataga attttactca gacataaaaa aaacccggca taggggaccg
      841 ggaagaggat agtctgccgt ctccagacta ataaaccgtt ataacactcc ctgttggcac
      901 gggaaacttt gtgctctcag t
//
LOCUS       DAZRUF010000492          911 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZRUF010000492 DAZRUF010000000
VERSION     DAZRUF010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.495"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(331..>911)
                     /locus_tag="ACHXOL_004428"
     CDS             complement(331..>911)
                     /locus_tag="ACHXOL_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR6811210.1"
                     /translation="RLYLAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCLPQNSELIY
                     CWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEP
                     GTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIA
                     RENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          276 a          182 c          164 g          289 t
ORIGIN      
        1 actagattta ataataacga actagattta ataacaacga tatccgtacc tatccggata
       61 tctaatgtca taagcatccc tttaattatc ccctcaacct cttgtaactt tcccactctt
      121 gaactccaca tagtccaaac gccggatgca aaaaaatcac acgtgcattt ctgaaattgt
      181 gatggaggaa gatagtatca attatatgtg cttataaacg tccccgatta gcgcagaagt
      241 caccggattg ttcaggctcc aataggttga gtatgccgta tttattttcc aaaataaaaa
      301 tcgatgctgt tgctccttca taaaatactt ttactctctg gacgcttttc ttagctctct
      361 tggtgttatc ttaaaacgat tatggaacct ttcagtaaaa cgagaaggac acttgtaacc
      421 attttccctg gcaatctcgc tgatgggttt tactgtcgtt tgtatagcag acaacgcatt
      481 atttaacctc acgtcgtcca atatactttg aaaacttact ccctcgcttg ccagacggcg
      541 atgcaatgtg gaaacagaaa tgtagagata tctcgctacc ttgtttgctg tccattttgt
      601 tccaggttcg gatagcatta ggttataaca acgacttatt aatgattgtt tactatctga
      661 taaaagtaaa caattaacat gatgtgctcc taatgaaagt agaacgccca ttgctaagtg
      721 ttcctgaatt tgcgttgaga agcctcggga aatagatgtt tttagttgct cccaacaata
      781 tattaactcg gaattctgag gtaagcagaa acctgtcgtg ttacgaattt gatcggttat
      841 cgcataaaga ttttggaact tttcaattag atcaatggga taataaagca tttctgcaag
      901 ataaagacgg t
//
LOCUS       DAZRUF010000493          891 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZRUF010000493 DAZRUF010000000
VERSION     DAZRUF010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.496"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            63..863
                     /gene="tcyJ"
                     /locus_tag="ACHXOL_004429"
     CDS             63..863
                     /gene="tcyJ"
                     /locus_tag="ACHXOL_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR6811211.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          251 a          181 c          251 g          208 t
ORIGIN      
        1 tttttgtgtt tacaaacaat ggctctacac tgcaaacaga cataacaaca ttcggggtga
       61 atatgaaatt agcacatctg ggacgtcagg cattgatggg tgtgatggcc gtggcgctgg
      121 ttgcgggtat gagcgttaaa agttttgcag atgaaggtct gcttaataaa gtcaaagagc
      181 gcggcacgct gctggtaggg ctggaaggaa cttatccgcc gttcagtttt cagggggatg
      241 acggcaaatt aaccggtttt gaagtggaat ttgcccaaca gctggcaaaa catcttggcg
      301 ttgaggcgtc actaaaaccg accaaatggg acggtatgct ggcgtcgctg gactctaaac
      361 gcattgatgt ggtgattaac caggtcacca tttctgacga gcgcaagaaa aaatacgatt
      421 tttctacccc ttacaccatt tccggtattc aggcgctggt gaaaaaaggt aacgaaggca
      481 ccattaaaac agccgatgat ctgaaaggca aaaaagtggg tgtcggtctg ggcaccaact
      541 atgaagagtg gctgcggcag aatgttcagg gtgtcgatgt gcgtacctat gatgatgacc
      601 cgaccaaata tcaggatctg cgcgtaggcc gtatcgatgc gatcctcgtt gaccgtctgg
      661 cggcgctgga tctggtgaag aaaaccaacg atacgctggc agtaaccggt gaagcatttt
      721 cccgtcagga gtctggcgtg gcgctgcgta aaggaaatga agatctgctg aaagcagtga
      781 atgatgcaat tgcggaaatg caaaaagatg gcactctgca agccctttcc gaaaaatggt
      841 ttggtgctga tgtgaccaaa taatcagcat aatgacaaaa aagggcgctt t
//
LOCUS       DAZRUF010000494          889 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZRUF010000494 DAZRUF010000000
VERSION     DAZRUF010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.497"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(19..543)
                     /gene="ygaP"
                     /locus_tag="ACHXOL_004430"
     CDS             complement(19..543)
                     /gene="ygaP"
                     /locus_tag="ACHXOL_004430"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR6811212.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(553..852)
                     /locus_tag="ACHXOL_004431"
     CDS             complement(553..852)
                     /locus_tag="ACHXOL_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR6811213.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          224 a          222 c          204 g          239 t
ORIGIN      
        1 cgcgtccggc gtcgcttctc aagctcgttg gttccacggc atcttatcta acaaccttgc
       61 cattccacaa aaaccgctga ttcctgcaaa cagtaacccg gcaccaacaa agccacttaa
      121 taagaagaaa ccgctattta cggtataacc cagtacaacg ccgattaata ttaaaccacc
      181 cgcagcgatc tgcacctggc gcattaacgg caagggttga gatttattta ccgctactgg
      241 caatccagct cttttccagc catcaatccc atcttcgagt aaaaatattt ctgcgggggc
      301 ggcaatcgct gctaatttat cggcgttatt actggtgcgt ttacctgcct ggcagtggaa
      361 tataatttgc tcgcgacgta atttaggtgg aagacctgac tgttccagca cggataatgg
      421 agccagatct gcttcaggaa tatgttcacg aagatattca tcagcgtcac gaatatcgat
      481 taactttgcg ccgcgtgcga ttaattcttg tgcatcatgc ggcgaaatgg ttgtcaaagc
      541 catagtgact ccttacggac aatagacatt tttcagggtg gcgataatgg catttaccgc
      601 ctcattttta atggaatata gaatgcgttg ggcatcccgt tggctgtcga taagcccttc
      661 gtcccgcata cgagcgagat gctgtgatgt cgcagaggca ctcagtccgg taatgcgcgt
      721 cagctctccc gcgctggtgc cgggggaacc gctaagcatg cacagaatca gcaatcgttt
      781 aggatggctc attgctttca ataaggccgc tgcctgttcg gcactggcct gtaattgcgc
      841 gagttcagtc atatttattt aagtattttc taaattaagt aagctctaa
//
LOCUS       DAZRUF010000495          889 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZRUF010000495 DAZRUF010000000
VERSION     DAZRUF010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.498"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..569)
                     /locus_tag="ACHXOL_004432"
     CDS             complement(<1..569)
                     /locus_tag="ACHXOL_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR6811214.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLA"
     gene            complement(569..>889)
                     /locus_tag="ACHXOL_004433"
     CDS             complement(569..>889)
                     /locus_tag="ACHXOL_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR6811215.1"
                     /translation="AAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAGSVI
                     VTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLKQPA
                     QEAS"
BASE COUNT          297 a          210 c          194 g          188 t
ORIGIN      
        1 gcaagaccca ttaagatccc tggcaaatat cccgccataa atagcgatgc tacagaaacg
       61 ccgccagctg tcagggaaaa cacaattaac acgtttgagg gcggtatcag taatccagta
      121 atacaagatg agacgtttac agcggtagaa aatgccggat cataaccttg tttggtctga
      181 atgggattca gtgtgccacc aacggcagca gctgccgcaa cggctgaacc agaaatggaa
      241 ccaaacatca tattcgccag gacgttcaca tgaccaagtg aaccaggaac ccgaccaacc
      301 attacctggg cgaggttaat caaacgtatc gcaataccgc cgctgttcat cagcgttccg
      361 gcaaagatga aaaaggggat tgccaatagg gcaaagttat ccagtccatt agccagtcgc
      421 tgcgaaacag tgattgccgc gatatcccag ggaaacatca aaagcattga agctacagtg
      481 gcaataccga ttgagaacga aatgggcaca ccaataaaga ccagcacaaa aaaacagcca
      541 aacagtgtaa tcgcgatata actatccatt aactggcctc ctgcgcaggt tgtttcagtt
      601 gttgaatgct ttgattaata aaacacaagg cataaaacat catcaccaat ccacttaatg
      661 gcagaatgat atatacgtat cccatcggta tttgcatcgc ggcagatacc tgtgatgttg
      721 ccagcgtttt atcaattaat ttcaggccac cagtcacaat gaccgaacca gcaaaaccta
      781 aaataagaac gttaatgact atactaagaa gtaattgctt gcgcttattc agggcgagag
      841 caaataaatc gatagagaga tgtctctgcg cgccgacggt atatgcagc
//
LOCUS       DAZRUF010000496          886 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZRUF010000496 DAZRUF010000000
VERSION     DAZRUF010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.499"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..183)
                     /locus_tag="ACHXOL_004434"
     CDS             complement(<1..183)
                     /locus_tag="ACHXOL_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811216.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSA"
     gene            complement(225..762)
                     /locus_tag="ACHXOL_004435"
                     /pseudo
     CDS             complement(225..762)
                     /locus_tag="ACHXOL_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
BASE COUNT          245 a          177 c          215 g          249 t
ORIGIN      
        1 ggcggataaa tcgacagatt cagcaagatc attgcttaaa aaccgtggat taaaataata
       61 ctctgcgtca acgggaaata ccgcagttgc taagccaagg gcaatggtaa gccgtgcaaa
      121 gcgaggcagt attaacacct gatgcatacg atatgcctta aatgcggcac gaaaggctgt
      181 cattattaat tccgattgag tttattatta attaggtcac tggcttactg catggttgcc
      241 ttaatacgcg gagttaatgc accgtaatca ttgatcgtct gaaaactgat atccccggtt
      301 gcagcatgcg ggatatccac cgatacttca ccttttggcg gcaccatggt gtttgggaga
      361 ttgctatttc ctgctttcat atttgttacg gtaatgaaat agggcgttgg attaataaga
      421 gtgagtttgc taccgctacg acgaaaccga agcattgcag gcgcttcttc aggtgccatt
      481 gccaggttcg tggggcgata aaacattttt atgcggctaa taattgccag ctgtaatgta
      541 ttctcattct tctggtcttt ttccatagca ggaatagctt taacattcac ccagaacagg
      601 ctttcacggt cgcctggtaa ctgatgatta gtagcgttga ttatccgcag cgtattccag
      661 tgcgacagct gcgttggcaa cggtgctatg gcaagcaaag agcaataaaa gtaacactgt
      721 cgccataaag gaaatagagt agggggtacg cgtagtttgc atctgatgca ttctccatgt
      781 gttaagagga taagcgggaa aaccgttaca tcagacaatc gattaatgtt gctaatacat
      841 tggcgagtcg ccgggtttcc agacttaatg cgtttgtaat aaatgt
//
LOCUS       DAZRUF010000497          884 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZRUF010000497 DAZRUF010000000
VERSION     DAZRUF010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.500"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            462..809
                     /gene="tnpB"
                     /locus_tag="ACHXOL_004436"
     CDS             462..809
                     /gene="tnpB"
                     /locus_tag="ACHXOL_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR6811217.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          223 a          239 c          228 g          194 t
ORIGIN      
        1 agcctgaacc gtctggtcag aatctgacga attagacaaa gtggtgtcca ccaaataagt
       61 agtgggaacc aaagtgtcag atatgcagaa aaatgtgact cccggcaggc gaaaaggctg
      121 ccctaattat cctcccgaat ttaaacagca gctcgttgct gcctcctgtg aacccgggat
      181 atccatctca aaacttgctc ttgaaaaaat ggcattaacg ccaatctgtt gttcaaatgg
      241 cgacaacaat ggcgcgaggg aaagctgcta ttaccttctt cagagagccc ccagctactt
      301 cctgtgactc tcgatgcagc tgccgaacag ccagaatcgc tcgcagagga cccgaaaacc
      361 ctcagtatca gcagtgaggt aacgttccgg cacgggacgc tccgcttcaa tggcaatgtc
      421 agcgaaaagc tcctgactct gctgatacag gaactgaagc gatgatcccg ttaccttccg
      481 ggatcaaaat ttggctggtt gccggtatca ccgatatgag aaatggcttc aacggcctgg
      541 ctgcgaaagt acagacggcg ctgaaagacg atcccatgtc cggccatgtt ttcattttcc
      601 ggggccgcag cggcagtcag gttaaactgc tgtggtccac cagtgacgga ctgtgcctcc
      661 tgaccaaacg gctggagcgt gggcgcttcg cctggccgtc agcccgtgat ggcaaagtgt
      721 tccttacaca ggcgcagctg gcgatgctgc tggaaggtat cgactggcga cagcctaagc
      781 ggctgctgac ctccctgacc atgctgtaaa tctctttatc ctggttgtca cagaataagc
      841 ccggtaaaat acgggcttat gaacgacatc tcttctgacg acat
//
LOCUS       DAZRUF010000498          877 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZRUF010000498 DAZRUF010000000
VERSION     DAZRUF010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.501"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>877
                     /gene="cadA"
                     /locus_tag="ACHXOL_004437"
     CDS             <1..>877
                     /gene="cadA"
                     /locus_tag="ACHXOL_004437"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR6811218.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKELH"
BASE COUNT          238 a          221 c          212 g          206 t
ORIGIN      
        1 caacttctca ccgatttacg aaggtaaatg cggtatgagc ggtggccgtg tagaagggaa
       61 agtgatttac gaaacccagt ccactcacaa actgctggcg gcgttctctc aggcttccat
      121 gatccacgtt aaaggtgacg taaacgaaga aacctttaac gaagcctaca tgatgcacac
      181 caccacttct cctcactacg gtatcgtggc gtccactgaa accgctgcgg cgatgatgaa
      241 aggcaatgca ggtaaacgtt tgatcaacgg ttccattgaa cgtgcgatca aattccgtaa
      301 agagatcaaa cgtctgagaa cggaatctga tggctggttc tttgatgtat ggcagccgga
      361 tcatatcgat acgactgaat gctggccgct gcgttctgac agcacctggc acggcttcaa
      421 aaacatcgat aacgagcaca tgtatcttga cccgatcaaa gtcaccctgc tgactccggg
      481 gatggaaaaa gacggcacca tgagcaactt tggtattccg gccagcatcg tggcgaaata
      541 cctcgacgaa catggcatcg ttgttgagaa aaccggtccg tataacctgc tgttcctgtt
      601 cagcatcggt atcgataaga ccaaagcact gagcctgctg cgtgctctga ctgacttcaa
      661 acgtgcgttc gacctgaacc tgcgtgtgaa aaacatgctg ccgtctctgt atcgtgaaga
      721 tcctgaattc tatgaaaaca tgcgtattca ggaactggct cagaatatcc acaaactgat
      781 tgttcaccac aatctgccgg atctgatgta tcgcgcattt gaagtgctgc cgactatggt
      841 aatgactccg tatgctgcgt tccagaaaga gctgcac
//
LOCUS       DAZRUF010000499          860 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZRUF010000499 DAZRUF010000000
VERSION     DAZRUF010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.502"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..595)
                     /locus_tag="ACHXOL_004438"
     CDS             complement(<1..595)
                     /locus_tag="ACHXOL_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811219.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYACFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT          250 a          186 c          149 g          275 t
ORIGIN      
        1 tcagcttagc cagtcttgat ttcagcaaat taatctcatt ttctcttgct ttcatcagtt
       61 tctctttggc aatttcttca agctcagctt gtgatttatc agcaacaatt agagacagtc
      121 gatgtggtgc tctgacagct ctttctgtac gaacaactct ttgaccttct ggtaaatctg
      181 caatcacttt accaatctct acagggtcat acccccaacc gtcacggtct agtcgagaga
      241 aacaagcata agaattggtt tgaagctcca tcgaaataac catttcgtca atttcgtctt
      301 caagcttgga ctgaattttc tcgtaaacac ttctcattgc ttcttgagag tctttaatag
      361 cttggaaata tcggcgttgt agcttcaact tcaaatcaag ttcacaccgt tcgttcatag
      421 aaaaatcaat ttcaactctt ggaatctcga gtggttctgg tggaatatat ggacctttat
      481 ttacttcgtg tgattcccgc caagctctat caaaagattc acgcaaagtt aattcgtgaa
      541 tatcaagtgc acttggtctg tgttcgataa gcatttcatg ttcatttcta atcattcgtt
      601 caccccttag tttatttgtt atttcaattt atagaagcac actcagcaat cagaggtgaa
      661 gagtctgact gaacaagaac ctgccaagtg tgcttttaaa aactgaaaaa gccctaaaca
      721 tttctgtgaa gggcaaagga ttcaataatg aatgaacaag agtacttttt gagatagcct
      781 ctcagactaa cctgaaaggc catgacgaaa agcattgcac ctttccaact cagaatatcc
      841 atccatcgga ttattgaaag
//
LOCUS       DAZRUF010000500          853 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZRUF010000500 DAZRUF010000000
VERSION     DAZRUF010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.503"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..851
                     /locus_tag="ACHXOL_004439"
     CDS             <1..851
                     /locus_tag="ACHXOL_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR6811220.1"
                     /translation="NWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGDFGY
                     QRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEKMAA
                     IHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGALHS
                     GTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENP
                     KFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFPGGK
                     HGLAKV"
BASE COUNT          230 a          208 c          239 g          176 t
ORIGIN      
        1 ttaactggtt cacgcagtcg ggtgttgagg gactgaaatc attacctgcc gggcgtgccc
       61 gtcgctggcc gtttgagcat atctgcacac tgttacgtga gctggtaaaa cattctcccg
      121 gcgactttgg ctaccagcgt tcacgctgga gtacagaact gctggcaata aaaatcaatg
      181 agataaccgg ttgccagtta aatgccggaa ccgttcgccg ctggttgccg tctgcgggga
      241 ttgtgtggcg aagggctgcg ccaactctgc gtatccgtga cccgcataaa gatgaaaaga
      301 tggcagcaat ccataaagca ctggacgaat gcagcgcaga gcatccggtc ttttatgaag
      361 atgaagtgga tatccatctt aatcccaaaa tcggtgcgga ctggcaactg cgcggacagc
      421 aaaaacgggt ggtcacgccg ggacagaatg aaaaatatta tctggccgga gcgctgcaca
      481 gcgggacagg taaagtcagc tgtgtgggcg gcaacagcaa aagttcggcg ctgttcatca
      541 gcctgctgaa gcggcttaaa gcgacatacc gtcgggcgaa aaccatcacg ctgatcgtgg
      601 acaactacat tatccacaaa agccgggaaa cacagagctg gctgaaggag aacccgaagt
      661 tcagggtcat ttatcagccg gtttactcgc catgggtgaa tcatgttgaa cggctatggc
      721 aggcacttca cgacacaata acgcgtaatc atcagtgcag ctcaatgtgg caactgttga
      781 aaaaagttcg ccattttatg gaaaccgtca gcccattccc cggaggcaaa catgggctgg
      841 caaaagtgta gcg
//
LOCUS       DAZRUF010000501          843 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZRUF010000501 DAZRUF010000000
VERSION     DAZRUF010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.504"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..290
                     /locus_tag="ACHXOL_004440"
     CDS             <1..290
                     /locus_tag="ACHXOL_004440"
                     /inference="COORDINATES: protein motif:HMM:NF019442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR6811221.1"
                     /translation="KINHARQYFVYLLAVILQEYDMYLTLKEWNARQLRPRSPETVRR
                     WVRECKIFPPPIKDGREYLFHESAKKITPQTTGGLLQRIRNGRTKKKLKHT"
     gene            256..>843
                     /locus_tag="ACHXOL_004441"
     CDS             256..>843
                     /locus_tag="ACHXOL_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFR6811222.1"
                     /translation="MAGRRRNSNTRDLPPNLYNRGGYYSYRDPRSGREFGIGRDKRIA
                     VNEAIAANMELLSTQHISLLDRIKGNSVTLFCELILNFRNEIERRQLSTNTMKRHNQR
                     LKIISEYFGGVPVKNIGIREVYSFLEIRAAGSKFAIANQYRALLSDIFKTAIASGLAE
                     EDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNE"
BASE COUNT          290 a          158 c          189 g          206 t
ORIGIN      
        1 taaaaatcaa ccacgcccgc cagtattttg tatatttact ggcggtcata ttacaggaat
       61 atgacatgta tttgacactc aaagaatgga acgcccgtca gttacgtcca cgaagcccag
      121 aaacggttcg ccgctgggtg cgagaatgta agatttttcc accaccgata aaggatgggc
      181 gtgaatattt atttcatgag tcagcaaaaa aaattacacc tcaaacgact ggcgggcttt
      241 tgcagagaat cagaaatggc cggacgaaga agaaactcaa acacacgtga tctaccaccc
      301 aatctttaca atcgaggtgg atattatagt tatcgtgacc cacgttcagg aagggagttt
      361 ggaatcggaa gagacaaaag aattgcagtt aatgaggcta ttgcggcaaa tatggaatta
      421 ttatccacgc aacatataag cttactggat agaataaaag gtaatagcgt aacattattc
      481 tgcgagctga tattaaattt cagaaatgaa atagagcgta gacaattaag cacaaacacc
      541 atgaaaagac acaaccagag gttgaaaatc ataagtgaat actttggtgg tgttccggtt
      601 aaaaatatag gcataaggga ggtgtacagc tttcttgaaa tcagagcagc tgggagtaaa
      661 tttgctattg ctaaccagta cagggcgctt ttatcagaca tttttaaaac agctatagct
      721 tctggtcttg ccgaagaaga tccagcttct gcaaccaggc cattcagaac tgaagtgaag
      781 cgcagtcgac tgttaattga cgagtatctg ttaatcagga aaatagcaga cgtccagaac
      841 gaa
//
LOCUS       DAZRUF010000502          841 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZRUF010000502 DAZRUF010000000
VERSION     DAZRUF010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.505"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..721
                     /gene="ydeO"
                     /locus_tag="ACHXOL_004442"
     CDS             <1..721
                     /gene="ydeO"
                     /locus_tag="ACHXOL_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR6811223.1"
                     /translation="NANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFTENTI
                     KKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLS
                     IFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQE
                     QTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSK
                     EYRCQSHMGMNTGNTMSALAI"
BASE COUNT          259 a          166 c          170 g          246 t
ORIGIN      
        1 caacgctaac attttagata aagattacgc cttctctgac ggcgagatcc tgatggtaga
       61 taacgctgtt cgtacgcatt ttgaacctta tgagcggcat tttaaagaga tcggatttac
      121 tgaaaatacc attaaaaaat atctacaatg cactaacatc cagacagtga cggtgcctgt
      181 tcctgcgaag tttttacgtg cttcaaatgt accgactgga ttgcttaatg aaatgattgc
      241 ttatcttaac tcggaagaac gcaatcatca taatttttca gaacttttgc ttttttcttg
      301 cctgtctatt tttgccgcat gcaaaggttt cattacacta ttaactaacg gtgtgctatc
      361 cgtttccgga aaagtgagaa atattgtcaa catgaagctg gcgcacccat ggaagctgaa
      421 agatatttgt gactgcctgt acatcagtga aagcctgttg aagaaaaaac taaagcaaga
      481 gcaaacgaca ttctcacaga ttctattaga tgccagaatg cagcacgcaa aaaatttgat
      541 acgcgtagaa ggttcagtca ataaaattgc cgaacaatgt ggttatgcca gtacatctta
      601 ttttatttat gcgttccgca aacatttcgg caacagtccg aagagagttt ctaaggagta
      661 ccgttgtcaa agtcacatgg gtatgaatac gggcaacacg atgagtgctt tagctatttg
      721 attatttgct aacgagtagt caaccacgca cgctgcgtaa gaattaaatg gggcagccat
      781 tccctgcccc gcgttgtttt taggcgatat atttattgaa ataaataagt gacatccatc
      841 a
//
LOCUS       DAZRUF010000503          841 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZRUF010000503 DAZRUF010000000
VERSION     DAZRUF010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.506"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..265)
                     /locus_tag="ACHXOL_004443"
     CDS             complement(<1..265)
                     /locus_tag="ACHXOL_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFR6811224.1"
                     /translation="MNNCIFFSISASTVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAG"
     gene            complement(282..>841)
                     /locus_tag="ACHXOL_004444"
     CDS             complement(282..>841)
                     /locus_tag="ACHXOL_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR6811225.1"
                     /translation="RCKTRWTRCTRRREKIGLKGGWNFYQYPLNPITDIDPLGLATCL
                     YSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRGPIPQGV
                     WSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLNAFGPSLGPRFCSEGCIT
                     GSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          241 a          180 c          147 g          273 t
ORIGIN      
        1 gtccagcatc tattttgtac ctcattatat tagataagga tgtgagaaat tccttgttat
       61 cattcgcagc catatatgcc agtccgagtt cagcctctgc ggccttacag gcaaaatagc
      121 ttagtgaaca cgcttttcgt ccctctttag tactggctga tattagtgcc cataatattg
      181 ctttgtctgc caaatctaac tctctctgaa attgtcttgc agtaactgta ctcgcagaaa
      241 tagagaaaaa aatacaattg ttaataactt tgccatattt tttagtccgt aactgttaat
      301 gtgctatctg gctctgaaaa tattaactca tttaattttt gcatgtcatt agatgaacca
      361 gttatacaac cctcggaaca gaatcgaggt cctaaagacg gaccgaaagc gtttaaacaa
      421 ctttgtgtta aaaatccatc acgattatat gtttctgtat ttgctgacgg tactaaacgt
      481 ataccattag gttttctatt ggttgctcca ggaccattaa cattccagct ccatacccct
      541 tgaggtattg gacctctatt ttgtaagtgt gtacaaccag gattattttt acattgcata
      601 ttattaccat tattaccaga ggcgacagga atagtaagca catcataaga gttatcatca
      661 ttttgcgtat ctgaaacgca tgaaagcata cttaaagtta tgctgtataa acaggtcgct
      721 aatcccaacg ggtcaatatc agttatcgga ttcaacggat actgataaaa attccatccc
      781 cccttcagtc caatcttttc gcgccggcgt gtacaccggg tccatctggt tttgcatctg
      841 c
//
LOCUS       DAZRUF010000504          839 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZRUF010000504 DAZRUF010000000
VERSION     DAZRUF010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.507"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            11..811
                     /locus_tag="ACHXOL_004445"
                     /pseudo
     CDS             11..811
                     /locus_tag="ACHXOL_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          237 a          165 c          206 g          231 t
ORIGIN      
        1 ggaatacata atgaaaagaa aagttctggc aatgctggtc ccagcgttat tagttgctgg
       61 cgcagcaaat gcggctgaaa tttataataa aaatggcaat aaactggatc tgtacggaaa
      121 agttgatgct cgtcatactt tttccgataa ccctggcgat gacggcgatg aaactatcat
      181 cgagttaggc ttcaaaggtg agacccaaat tacagatcag ttaactggtt atggtcaagc
      241 actgaccaaa acaaaggcta gcgacactga aggttctgat aatacttatg tcaaacttgc
      301 ttttgcaggt ctgaagttcg gcgagatggg ctcctttgac tatggtcgta actatggtgt
      361 gatttacgac gttgaagcct ggacagatat gcttcctgta ttcggcggcg actcctatac
      421 ctggactgat aacttcatgg caggccgtgc aaatggggta gctacttacc gcaatagtga
      481 tttcttcggt ctggtggaag gtctgaactt tgcattgcag tatcaaggta acaacgaagg
      541 cagcaatgca ggagaagatc aagaaggtac taaaaatggt cacgaagacg ttcgcttcca
      601 gaacggtgat ggttttggtc tttccacttc atatgacttt gacttcggtt tgagcttagg
      661 tgccgcttac tcaaactctg accgtaccaa ttctcaagta gctcttggcg ggtatcacta
      721 caatgaatac agcaaatttg ctggtggtga tacagctgaa gcatggacat ttggtgcaaa
      781 atacgacgcc aacaatgttt atcttgcaat gggtgatgct gccaacttac tgatttagt
//
LOCUS       DAZRUF010000505          838 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZRUF010000505 DAZRUF010000000
VERSION     DAZRUF010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.508"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..258)
                     /locus_tag="ACHXOL_004446"
     CDS             complement(<1..258)
                     /locus_tag="ACHXOL_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HFR6811226.1"
                     /translation="MSLGVNVTCFDVLPKGTNGFHWTDSLIDIDLDGYKYISFPDIIS
                     GSLPSYSEQKGMSNDAINFKISNVNASVRALALGGFLKDAQM"
     gene            complement(351..>838)
                     /locus_tag="ACHXOL_004447"
     CDS             complement(351..>838)
                     /locus_tag="ACHXOL_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811227.1"
                     /translation="QTWTGQRIMRSTGIQYYQIQFTLNFNPASLNEVNSFLAQYAQGK
                     PFTMSLGLAGTYYGKQTGAVTATALTKPGNMVVPISTNTLAVGEWVQFSNHNKLYRIV
                     ERTSTSITIFPALQNTVQASEALKYNNLMIEAVLDPDNDYSLPIGNIMNITLKATENI
                     I"
BASE COUNT          254 a          157 c          157 g          270 t
ORIGIN      
        1 catctgtgca tctttcaaga aaccaccaag ggcaagagca cgaaccgagg cgtttacatt
       61 gctaatttta aaattgatag catcattaga catgcccttt tgttcagaat atgaaggtaa
      121 tgaaccactg ataatgtcgg ggaaacttat gtacttataa ccatccagat ctatatcaat
      181 tagtgaatcc gtccagtgaa aaccattagt accttttggt agtacatcaa aacaagtaac
      241 attaacgcct aagctcataa tttccattag tgttaatctt gtcttggttg tacctctgac
      301 taacttccag tacttaagaa gattagcatt agtatatata cctgagtcca ttagatgata
      361 ttctccgttg ctttcaatgt aatattcatg atgttcccaa taggcaatga ataatcattg
      421 tctggatcta atacagcctc gatcatgaga ttgttatatt tgagtgcttc acttgcctgt
      481 actgtatttt gtagagcagg gaaaatagta attgaagtac tggtacgttc tacgattcga
      541 tatagcttat tgtgattgga aaactgtacc cactcaccca cagctaatgt gttagtactg
      601 ataggtacaa ccatattacc cggtttagtg agagcagtag cggttactgc accagtctgt
      661 ttaccgtaat atgtacctgc aagccccaat gacatagtga aaggtttgcc ctgtgcatat
      721 tgagctaaaa agctattcac ttcatttagt gatgcaggat taaaattaag ggtgaactga
      781 atttgatagt attgaattcc agtacttctc atgatacgtt gacctgtcca tgtttgat
//
LOCUS       DAZRUF010000506          837 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZRUF010000506 DAZRUF010000000
VERSION     DAZRUF010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.509"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>837
                     /gene="ipaH3"
                     /locus_tag="ACHXOL_004448"
     CDS             <1..>837
                     /gene="ipaH3"
                     /locus_tag="ACHXOL_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR6811228.1"
                     /translation="FSAWDKWEKQALPGENRNEAVSLLKECLINQFSELQLNRLNLSS
                     LPDNLPPQITVLEITQNALISLPELPASLEYLDACDNHLSTLPELPASLKHLDVDNNQ
                     LTMLPELPALLEYINADNNQLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVST
                     NLLESLPAVPVRNHHSEETEIFFRCRENRITYIPENILSLDPTCTIILEDNPLSSRIR
                     ESLSQQTAQPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEH
                     EE"
BASE COUNT          250 a          228 c          153 g          206 t
ORIGIN      
        1 cttttcagca tgggataaat gggaaaaaca agcgctcccc ggtgaaaatc ggaatgaagc
       61 ggtctcccta cttaaagaat gtctcatcaa tcagttcagt gagcttcaac tgaatcgttt
      121 aaatctgtcc tcgctacctg acaacttacc acctcaaatc actgttctgg aaattactca
      181 gaatgcccta atatcattac cagaattgcc agcatcgctg gaataccttg acgcctgtga
      241 caatcacctg tcaacacttc ctgaattacc cgcatctctg aaacatcttg atgtagataa
      301 caaccaacta accatgcttc ctgaattgcc tgcattgctg gaatatatta atgcagataa
      361 caatcagcta accatgcttc ctgaattacc tacatcgctg gaagtgctct cagtaagaaa
      421 taaccagctg acatttctcc ctgagttacc tgaatcactg gaagcgctcg atgtaagtac
      481 taatcttctg gaaagcctac cagccgtacc tgtaagaaat catcactcag aggaaaccga
      541 gatatttttc cggtgccgcg agaatcgcat cacatacatt ccggaaaata tacttagcct
      601 tgatccgacc tgcactatca tcctcgaaga caatcctctg tcctcacgga tcagggagtc
      661 tctgtcgcaa caaaccgccc aaccggacta ccacggccca cggatttact tctccatgag
      721 tgacggacaa cagaatacac tccatcgccc cctggctgat gccgtgacag catggttccc
      781 ggaaaacaaa caatctgatg tatcacagat atggcatgct tttgaacatg aagagca
//
LOCUS       DAZRUF010000507          832 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZRUF010000507 DAZRUF010000000
VERSION     DAZRUF010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.510"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(354..461)
                     /locus_tag="ACHXOL_004449"
     CDS             complement(354..461)
                     /locus_tag="ACHXOL_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR6811229.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          168 a          251 c          195 g          218 t
ORIGIN      
        1 caacgtttcg cggctattct tgagttgttc aggttcaaga atggcccccg tgatgttgtc
       61 aggtgcatac ctgcaacgtg cgggggtttt ctctctccag caaccaatgc caccagggat
      121 aaagcccccg caacattgcg cctcaccgga acccttccgg cttgacgctg attctacgac
      181 tgtttttctg ttccggaatc cgtcacgtct tcactctgtg ctgcgcattc cgcagaatat
      241 tatccgcttg caaccgttgc atttttctta cgaaaaaggc cgcagagttt cacccctgcg
      301 gcctgatccg ggcgcaaatt gccattacgg cagcctgacg cccgcatggc acgttacttc
      361 cgcttgttca gccagttcac gatcatactg gcaagaatac cagcaatgac cggagccgct
      421 aaatcatgcc agaaggccat gcccagttct gcgagcgtca tatagccgcc tgtattgtaa
      481 tgacaacgtt tcgcggctat tcttgtattg tttggatgca agaatggccc ccgtggtgtt
      541 gtcaggtgca tacctgcaac gtgcgggggt tttctctctc cagcaaccaa tgccaccagg
      601 gattaagccc ccgcaacatt gcgcctcacc ggaatccttc cggcttgacg ctgattctac
      661 gactgttttt ctgttccgga atccgtctca tcttcactct gtgctgcgca ttccgcagaa
      721 tattatccgc ttgcaaccgt tgcatttttc ttacgaaaaa ggccgcagag tttcactcct
      781 gcggcctggt ccgggcgcaa attgccatta cggcagcctg acgcccacat ga
//
LOCUS       DAZRUF010000508          830 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZRUF010000508 DAZRUF010000000
VERSION     DAZRUF010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.511"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..516
                     /locus_tag="ACHXOL_004450"
     CDS             <1..516
                     /locus_tag="ACHXOL_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR6811230.1"
                     /translation="QYGYDESTVRTWVEKGMPTDTDSNARSWIVDNVLKPLRDTNTKE
                     QIEQERLKKLSAERQLAELELAEKNGLVVSTEYIEQVLTEYLFQVKTAVRAIPSKTYL
                     ELFAQSDAKDLRDVLRTHIDKTLYQLGSMEFELPDDMEVIEDGNKQEEINEDTEESTT
                     DDTTAEDTENE"
     gene            560..>830
                     /locus_tag="ACHXOL_004451"
     CDS             560..>830
                     /locus_tag="ACHXOL_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR6811231.1"
                     /translation="MGLDWVPFSFQKEPMDIAQERSTKKIVLQSCSQLLKTTVLQSIA
                     FNLMANDPCNFAFGSSSESEVKKFKDGKFLPAIETSSVLKPLVTDK"
BASE COUNT          305 a          133 c          182 g          210 t
ORIGIN      
        1 cagtacggat acgatgaatc tacagttcga acttgggtag aaaagggaat gccgacagac
       61 actgattcaa acgcaagaag ttggattgta gataatgtac taaagccgtt gcgtgatact
      121 aatacaaaag aacaaattga acaagagcgt ttgaagaagc tttcagcaga acggcagtta
      181 gcagaattag aattagcaga aaagaatggg ctggtggtca gtactgaata tatagaacag
      241 gtactaacgg aatatctatt ccaggtcaaa acagcagtgc gtgcaatacc atcaaaaact
      301 tatcttgaat tatttgcaca atcagatgca aaggatttac gcgatgtatt aagaacgcat
      361 attgataaaa cattatacca attaggctca atggaatttg agttacccga tgacatggaa
      421 gtaatagagg atggaaacaa acaagaagaa attaacgagg atactgaaga aagtactacc
      481 gacgatacaa ccgccgaaga tacagaaaac gagtgagtgg attagtaatg gtgtagttaa
      541 atttgtggac gggccgaata tgggccttga ttgggttccg tttagtttcc aaaaagaacc
      601 aatggatata gctcaagagc gaagtactaa aaagattgta cttcaatctt gttcccagct
      661 tttgaaaact acagtactac aatcaattgc atttaaccta atggctaatg atccctgtaa
      721 ctttgctttt ggtagtagtt ctgaatcaga agtgaagaaa ttcaaggatg gtaaatttct
      781 tccagctatt gaaaccagtt cagtactaaa gccgttagta accgataaga
//
LOCUS       DAZRUF010000509          827 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZRUF010000509 DAZRUF010000000
VERSION     DAZRUF010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.512"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>827
                     /gene="htrE"
                     /locus_tag="ACHXOL_004452"
     CDS             <1..>827
                     /gene="htrE"
                     /locus_tag="ACHXOL_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR6811232.1"
                     /translation="RDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMKNYQN
                     YVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASGNYNW
                     MTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRMLPPT
                     LASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVTVEES
                     DGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNNYLT"
BASE COUNT          215 a          183 c          193 g          236 t
ORIGIN      
        1 ccagggatga aacactcggc aattgcctta atttgacgga aattatccct caggcttctg
       61 ttcgttatga cgttaacgag caacgtctgg atatagacgt tcctcaggca tgggtaatga
      121 aaaattacca aaactatgtt gatccatcgt tatgggaaaa tggcattaat gcggccatgt
      181 tgtcatacaa cctcaacgga tatcatagtg aaactcctgg tcgaagaaat gacagcattt
      241 atgctgcttt taacggtggg atgaatttag gtgcatggcg actgcgtgcc tcaggcaact
      301 acaactggat gaccgattct ggcagtaatt atgattttaa gaatcggtat atacagcgtg
      361 atatcgcctc gctgcgttct caactcattc ttggtgagtc ttatacaacg ggcgaaacct
      421 ttgattctgt cagtatccgc ggcattcgtt tatacagcga cagccgcatg ttgcctccga
      481 cattagcgag ttttgcgcct atcattcatg gcgttgctaa taccaacgcc aaagtcacta
      541 ttacgcaagg tggctataag atttatgaaa cgactgtgcc gccaggcgct ttcgtcattg
      601 atgatctgag tccgtcaggt tacggcagcg atcttattgt tactgtcgaa gaatccgatg
      661 gctcaaagcg gacattctcg caaccttttt catcggtcgt tcaaatgtta cgccctggcg
      721 ttggacgttg ggatattagc ggcggccagg tcttaaaaga tgatattcag gatgagccta
      781 atttatttca ggccagctac tactacggtc tgaataacta tctaacg
//
LOCUS       DAZRUF010000510          823 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZRUF010000510 DAZRUF010000000
VERSION     DAZRUF010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.513"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..354
                     /locus_tag="ACHXOL_004453"
     CDS             <1..354
                     /locus_tag="ACHXOL_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR6811233.1"
                     /translation="LDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQ
                     RNGGVQDVRYGYEMYYTPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRP
                     LQKLINGTWYNVASI"
     gene            357..>823
                     /locus_tag="ACHXOL_004454"
     CDS             357..>823
                     /locus_tag="ACHXOL_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR6811234.1"
                     /translation="MLHLKNITAGNPKTAEQYQLTKQYDVTWLFSEDGKNWYEEQKNF
                     ASDTIKMVYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQA"
BASE COUNT          230 a          181 c          224 g          188 t
ORIGIN      
        1 ctggatgttt atgccaatag cgtgcatgtg ttgcgttttc agagtggcag tatccagagt
       61 aataaagctg taaacgttac aggacgggta acaccgtcag actacggaaa ctttgatgcc
      121 cgttaccagc agcgaaatgg tggcgtgcag gatgtgcggt atggttatga aatgtattac
      181 acccccggca gtaacaccgt ttcgtggaca tttcgctcac cttcgggaca cgggctgtca
      241 gggatatcga tatcggatac cggccgtaac tcagcggata acgtcaacgg cgtgtattac
      301 cgaccgctgc aaaaactgat taatggcacc tggtataacg tagcgagtat ttaacaatgt
      361 tgcatttaaa aaatattacg gcaggtaatc cgaaaaccgc ggaacaatat cagctgacaa
      421 aacagtatga tgtcacctgg cttttttcgg aagacggcaa aaactggtat gaagaacaga
      481 agaactttgc cagtgacacc ataaaaatgg tttacaccgg agacgggcgc gtggtgtggg
      541 tcggtaagga tgtgacaggc attgaacccc gtaacgccag tgttattgaa gttcctgata
      601 ttaccgccaa ccgccgcatt accgcgccgg gttactggtt ttaccgcaat gatgaatttg
      661 tctttgacta cagactcaaa gcggaagatg agcgtgatgc ccttctggct caggtcagtg
      721 cccggacagg ggaatgggaa gaagacctgc tgctggggct aatcagcgac gaagaccggg
      781 aaaaactgaa agcgtaccgt atttacgcga aatcgctgca ggc
//
LOCUS       DAZRUF010000511          817 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZRUF010000511 DAZRUF010000000
VERSION     DAZRUF010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.514"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..273
                     /locus_tag="ACHXOL_004455"
     CDS             <1..273
                     /locus_tag="ACHXOL_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR6811235.1"
                     /translation="HLIRSNAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQK
                     YGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            535..>817
                     /locus_tag="ACHXOL_004456"
     CDS             535..>817
                     /locus_tag="ACHXOL_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811236.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSG"
BASE COUNT          287 a          132 c          157 g          241 t
ORIGIN      
        1 catctaatta gaagcaatgc tgtagcatcc acacctgtat atgaaaatta ttttaataac
       61 actactgctt tcagaccaaa acctgaagat atcttcaaat gtggacagac gctcaaaaaa
      121 actaaacaga aatatggcca ttatggatta ggggtagatc agcatcaaaa aattgataat
      181 tataccatgg ggctaaattc ctggaagata gcacctaacg aacgtggtga taaaaaaggt
      241 gtcccagggc ttagttgttt tcaacctcaa taaaacttca tgagtgctgt ggtatctctt
      301 tttaattttg ccagtatttc ataggttttc ttaatcgatt gtagaggcat gttctgatga
      361 cgcctctaca atctttaggg actttctctg ctatgagata agaaatttca tattatgctt
      421 taaggctgaa tgatttgtgc aaaatataat tattttccca tcgaacatga ggtgtatgtc
      481 ataagattaa atgttaacta tgtattcctc gcggtaaata atatgaggta aaagatgaaa
      541 attggaactg tggcaggcac taacgacagt acaacgacaa tcacaacaaa tgatatggtg
      601 caagagcatg ttaccaactt cactaaagaa ctgtttgggt atattgcaaa tggtataggt
      661 gatgatatat caagtattgc caggacaatg ctcggtgagg ttgtggaaaa aatagatgat
      721 tggcaaatag aacggttcca acaatctatt caagatgaca aaatttcatt taccatacaa
      781 acgaatcatt cagaaaaata tagtatgcta tcgggca
//
LOCUS       DAZRUF010000512          815 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZRUF010000512 DAZRUF010000000
VERSION     DAZRUF010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.515"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(246..>815)
                     /locus_tag="ACHXOL_004457"
     CDS             complement(246..>815)
                     /locus_tag="ACHXOL_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR6811237.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          259 a          203 c          174 g          179 t
ORIGIN      
        1 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
       61 atcagtaagt tggcagcatc acccgctttg gcttgataac cggataacaa cgtgcgcttt
      121 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
      181 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
      241 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
      301 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
      361 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
      421 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
      481 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
      541 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
      601 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
      661 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
      721 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
      781 cgtcgggata atgatttaac taagcgtggg aagaa
//
LOCUS       DAZRUF010000513          799 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZRUF010000513 DAZRUF010000000
VERSION     DAZRUF010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.516"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..741)
                     /locus_tag="ACHXOL_004458"
     CDS             complement(<1..741)
                     /locus_tag="ACHXOL_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR6811238.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSNHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          268 a          136 c          161 g          234 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga ttactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaatttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattttaga
//
LOCUS       DAZRUF010000514          795 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZRUF010000514 DAZRUF010000000
VERSION     DAZRUF010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.517"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>795
                     /locus_tag="ACHXOL_004459"
     CDS             <1..>795
                     /locus_tag="ACHXOL_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFR6811239.1"
                     /translation="ISISDFKRQLNDIKQNPLHDKEIKEMDNKNLLKDMVRAIKTGDK
                     SALEAYERGINGFVRAAVPSTNTTTAAGVVADDLQDQYIPELLKISALGELNTTVYSG
                     LAGRGTLSIPKAAGVSPVFKFYGEAEAQDDSIASFTKVTLSPKAFGGAIPLSKQAILT
                     APNIESFVQSELLRYAAQGLEQNVMDKVVAAAPVFNVETAGSITLEDVQGAVAKLAQA
                     NVDMRGVKAVMNAKTLSTLRQIAVLDNTAAKAMVEGYRSTEMWLAD"
BASE COUNT          245 a          153 c          167 g          230 t
ORIGIN      
        1 aatttcaatt tcagatttca aacgtcaatt gaatgacatt aaacaaaatc ctcttcatga
       61 taaggaaatc aaagaaatgg ataataaaaa tctattaaaa gatatggtac gtgccattaa
      121 aactggcgat aaatctgctc ttgaagcata tgaacgtggt attaatggtt ttgtacgtgc
      181 ggctgtacca agtactaata ctactaccgc tgctggcgtt gtagctgatg acctacaaga
      241 tcaatacatt ccagaactac taaaaatttc tgcacttggt gaactaaaca ccactgttta
      301 ttctggcctt gcaggtcgcg gtactctaag tattccaaaa gcggctggtg tatctccggt
      361 atttaaattc tatggtgaag cagaagcaca ggatgattct attgcaagct ttactaaagt
      421 aaccctaagc cctaaagctt ttggtggtgc tattccacta tccaaacaag caattctaac
      481 ggctccaaat attgaatcat ttgtacaaag tgaactacta cggtatgccg ctcagggtct
      541 tgagcaaaat gtaatggata aagtagttgc cgctgctccg gtgttcaatg ttgaaactgc
      601 tggttctatt actcttgaag atgttcaggg tgcagtagcg aagctggcac aggctaacgt
      661 tgatatgcgt ggcgttaaag ctgttatgaa cgctaaaacc ctaagtaccc tacgtcagat
      721 tgcagtactt gataacactg ctgctaaagc aatggttgaa ggttatcgca gtactgaaat
      781 gtggcttgct gatga
//
LOCUS       DAZRUF010000515          790 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZRUF010000515 DAZRUF010000000
VERSION     DAZRUF010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.518"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            52..790
                     /locus_tag="ACHXOL_004460"
                     /pseudo
     CDS             52..790
                     /locus_tag="ACHXOL_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          228 a          194 c          208 g          160 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt
//
LOCUS       DAZRUF010000516          789 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZRUF010000516 DAZRUF010000000
VERSION     DAZRUF010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.519"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHXOL_004461"
     CDS             complement(<1..458)
                     /locus_tag="ACHXOL_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR6811240.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIA"
     gene            complement(530..742)
                     /locus_tag="ACHXOL_004462"
     CDS             complement(530..742)
                     /locus_tag="ACHXOL_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811241.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          203 a          193 c          173 g          220 t
ORIGIN      
        1 gcgatggatt ttgcccgacg cccgcggcta ccgtccggat aaaaatgttc ggtgtaaaat
       61 ccagcctgaa tggttaccca ctcgcgaaaa gcctcaaacg actttagcaa tgccgtatcc
      121 cgggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc gacctactga ttcgcacagg taatcaacga aaccagacag cagttctcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaatcccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac
      361 tcacctgttt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgat cagttgtata tgcgccatcg tagttctccg ctggcgcagt
      481 agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac taacaggatg
      541 ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt accctcatcg
      601 gtaaaaaaat tgcctttcga ccattacgat acatcattga ttttttggtt tcagggaagt
      661 aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat ttactactaa
      721 atagtttctt tattttttcc acgatgcctc caggtttata agtacaaacg gttatatcaa
      781 catagagac
//
LOCUS       DAZRUF010000517          782 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZRUF010000517 DAZRUF010000000
VERSION     DAZRUF010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.520"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..207
                     /locus_tag="ACHXOL_004463"
     CDS             <1..207
                     /locus_tag="ACHXOL_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811242.1"
                     /translation="DAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGV
                     EYAFPDPQTQNETQQAGYDENIPV"
     gene            258..446
                     /locus_tag="ACHXOL_004464"
     CDS             258..446
                     /locus_tag="ACHXOL_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR6811243.1"
                     /translation="MYKITATIEKEGGSPASWTRYSKKKLTKSECEKMLSGEKEAGAS
                     REQKVKLINFNCEKLQSS"
     gene            553..>782
                     /locus_tag="ACHXOL_004465"
     CDS             553..>782
                     /locus_tag="ACHXOL_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR6811244.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSP"
BASE COUNT          275 a          158 c          175 g          174 t
ORIGIN      
        1 gacgccaaac gcgcaaaagc tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa
       61 agaatcaaag gtatggactg gccgacaatc aaagctggtc gtaatgcact atggattgaa
      121 ctggaaggcg ttgaatatgc attcccggat ccacaaacac aaaacgaaac gcaacaagca
      181 ggatacgatg agaatattcc ggtgtaaaac caagtgcatt ttggacacgg tttttgaaaa
      241 tattaatgga ggtgttcatg tataaaatta cagccactat tgaaaaggaa ggtggttctc
      301 ctgccagctg gacaagatac tcaaagaaaa aactaacgaa atcagaatgc gaaaaaatgc
      361 tctcagggga aaaagaagca ggcgcttcca gagagcagaa agtaaaactg ataaatttta
      421 attgcgagaa acttcagtcc tcgtgaattt cattatatcc agattagaac ttcatagctg
      481 attattaaaa atcaaccaca cccgccagta ttctgtatat ttactggcgg tcatatcgtg
      541 agaggtatgg caatgaatct tgtgacactc aaaacgtggg gaaaactcag atatccggat
      601 aacccaccat caatatcaac gctgagacga tgggcaagga atggaaacat ttatcctgca
      661 cctgaactac acgggaggag ttacagggtg gttccggagg ctttctatat caacccaaat
      721 aaggttgata ccgatataac acaccatcag cctaatgggc gacaagggag agacagtccg
      781 tt
//
LOCUS       DAZRUF010000518          779 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZRUF010000518 DAZRUF010000000
VERSION     DAZRUF010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.521"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            185..751
                     /gene="slp"
                     /locus_tag="ACHXOL_004466"
     CDS             185..751
                     /gene="slp"
                     /locus_tag="ACHXOL_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR6811245.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          235 a          164 c          173 g          207 t
ORIGIN      
        1 ggattatatt atggggcgat ggatactcat gccacgcccc aaaatcaatg gaattgaact
       61 aaacaaaagg gatataaaca tcagacaggt ttacgttact atcaggcata tcgcctcaga
      121 atcagatgaa aactataaag aaatatctat tatggtttta atatttgttg ataaggatag
      181 taacatgaac atgacaaaag gtgcactcat cctcagcctt tcatttttgc ttgccgcatg
      241 tagttcaatt ccgcaaaata tcaaaggcaa taaccaacct gatattcaaa aaagttttgt
      301 tgctgttcat aaccagccgg ggttatatgt tggtcaacaa gcgcgctttg gtgggaaggt
      361 tatcaacgtt atcaatggca aaacggatac gttgttagaa atcgctgtat taccgttgga
      421 tagctatgcg aaacctgata ttgaagccaa ctatcagggc cgactgctcg ccagacaaag
      481 cggcttcctt gacccagtga actatcgtaa tcactttgtt accatcctcg gtaccattca
      541 gggtgaacaa cctggcttta tcaataaagt cccgtataac ttcctggaag tgaatatgca
      601 gggcatccag gtgtggcatt tgagagaagt ggttaatacc acctataacc tgtgggatta
      661 cggctatggt gcattctggc cggaaccggg ctggggtgcg ccttactaca ccaatgcggt
      721 gagtcaggta acacctgagc tggtcaaata acaccaccga aagatgcaga tgctttctc
//
LOCUS       DAZRUF010000519          775 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZRUF010000519 DAZRUF010000000
VERSION     DAZRUF010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.522"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..343
                     /locus_tag="ACHXOL_004467"
     CDS             <1..343
                     /locus_tag="ACHXOL_004467"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR6811246.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(405..>775)
                     /locus_tag="ACHXOL_004468"
     CDS             complement(405..>775)
                     /locus_tag="ACHXOL_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HFR6811247.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          175 g          200 t
ORIGIN      
        1 tgcagatggt catttttgtg gcaatttgcc gaatacgacc catttccatg agcaaaccgt
       61 ggagttcccg atccagggag agatggttga tattgtggcg catggtgagt tgggcggcga
      121 cttttcgtta gtgcctgaca gctacgtgac gatggggccg aaaagcatca tggcggcgaa
      181 aaacttgctg ataattgtca gtggcgcagg taaagcgcag gcactgaaaa atgtgctgca
      241 aggtccggtt accgaagatg ttccagcttc cgtgttgcag ctgcatcctt cgctgatagt
      301 aatcgctgat aaagccgctg ccgcagaatt agcgctcggt taagctgaag aaataaggcc
      361 ggatgtatga attacgctcc ggcctgttct cattatttaa ataactacga aaaatcaccc
      421 cacagtctca gcttgccgca gtggagacag cggaagagat aaccctgaca atcaccaccc
      481 ttttgcaggc atttagctag atcagaattt ctaataccga aattctcaca atcttcttca
      541 aggttggcaa attcatcgag gcgatctttt atatcattcc agcccacata gccgataaaa
      601 gcacagaaat cgccacaatg cgcaagccag aattcctgtt gccatccatg ataacctggc
      661 gtgcgttcaa ccaactcttt cagcatttca tcaggatatg tgttcttaat accggcaaat
      721 tcgtcctctt catcatactc aaattcaaca ccttctatgc tggcatcatc ctgaa
//
LOCUS       DAZRUF010000520          769 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZRUF010000520 DAZRUF010000000
VERSION     DAZRUF010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.523"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            192..>769
                     /locus_tag="ACHXOL_004469"
     CDS             192..>769
                     /locus_tag="ACHXOL_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFR6811248.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSP"
BASE COUNT          168 a          235 c          199 g          167 t
ORIGIN      
        1 ccagccagac ccggcatgat tactgccccc agtcgtccat gatccggggg gtgatgtcac
       61 cgggtctggt ggggcgctgg taaccgctaa taggggtcag gtcaggcact tttgccggga
      121 ccgtctgtaa cgtggatgcc ggtacctgct ccccgtggtt atctggttaa cccatataca
      181 agggagacag aatgaccgaa tccagcgatt acgaatccgt ccaggtcttt atcggcgttg
      241 atgtcggtaa agatacgcat cacgctgttg ccattaatcg ttcaggtaaa cgcctgttcg
      301 ataaagcatt acccaacgac gaaaacaaac tcaggtcgct aatatctgac ctgaaacaac
      361 atggtcagat actgctggtt gttgatcagc cagctaccat cggtgcgtta cctgtcgccg
      421 ttgcccgctc agaaggagtc cttgtcggat acctccctgg actggccatg cgccgcatag
      481 ccgacttaca cgccggtgaa gctaaaactg atgctcgtga cgctgccatc attgccgaag
      541 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
      601 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      661 accgtatccg cggccttctg acccagatac atccggcact ggagcgcgtt ctcggtccga
      721 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacc
//
LOCUS       DAZRUF010000521          762 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZRUF010000521 DAZRUF010000000
VERSION     DAZRUF010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.524"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            72..>762
                     /locus_tag="ACHXOL_004470"
     CDS             72..>762
                     /locus_tag="ACHXOL_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR6811249.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQK"
BASE COUNT          254 a          132 c          167 g          209 t
ORIGIN      
        1 ataatatgcc gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg
       61 agatcattcc agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg
      121 gtgcaacgtc taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gctaaacaag
      181 gtgaacccga tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg
      241 cacaagttcc aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg
      301 aaacgttcgt gcagggacgt tatgaaggct ttggtcctaa tggctcaatg attatcgccg
      361 agacattgac ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa
      421 aaggcggcaa tatcggagcg gcaggttctg tcagctatat gtttgacaat gctggtgtga
      481 ttgtatttaa aggatcagac cctgaccata tttttgaaat tttacttgaa gctgaagttg
      541 atgttcgtga tgtgactgaa gaagaaggta acattgttat ttataccgaa cctactgacc
      601 ttcataaagg aatcgcggct ctaaaagcag ctggaatcac tgagttctca acaacagaat
      661 tagaaatgat tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag
      721 ggcttgttga tgcccttgaa gatgacgacg atgtacaaaa ag
//
LOCUS       DAZRUF010000522          758 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZRUF010000522 DAZRUF010000000
VERSION     DAZRUF010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.525"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            79..>758
                     /locus_tag="ACHXOL_004471"
     CDS             79..>758
                     /locus_tag="ACHXOL_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR6811250.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          237 a          162 c          130 g          229 t
ORIGIN      
        1 tcaatcctga aacattttat ttcagcttat tatttcccag tatcacattt tatcagaata
       61 tccttctgcg aggttattat gcttcctgta aataatcccc ccctatccac tggaaacgtc
      121 tctttttaca gaactacatc aatcgacaat gttcacaata attatctctc cgaatgggtt
      181 gaatggacta aaaacagcat ttccggagaa aacagggaaa ctgcttttac ccggctccaa
      241 ttatgtctgg agaacagtga aacatcgttg gacttatctt gtttaggtct cagatctcta
      301 ccacgattgc ctgacaatct tgatgaaatt aatgtaagca ataaccaact atcaatgctc
      361 cccgagctac caagggcatt gaaagagctg aatgcaagca gtaatcaatt atctgcactt
      421 cctgaattac cagtgtcgct ggaatatata aatgtgagtg ataaccattt gttcgcactt
      481 cctgaattac ctgcgtcact agaatatatt aatgtaagtg acaatcacct gtctgtactt
      541 ccgaggttac caatgtcatt ggaattactt gatgcagcca gaaatgcttt ggaagtaata
      601 ccagattttc cagaaagaga tgatcatatt ataagaatat tctggcttaa tcagaaccgg
      661 atcacggcaa ttccggaaag catacttggc ctcagttctg atagcgttgt caatcttaga
      721 gaaaatcaac tatctcccag aataatgcaa actttgtt
//
LOCUS       DAZRUF010000523          750 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZRUF010000523 DAZRUF010000000
VERSION     DAZRUF010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.526"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            189..>750
                     /gene="fimB"
                     /locus_tag="ACHXOL_004472"
     CDS             189..>750
                     /gene="fimB"
                     /locus_tag="ACHXOL_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFR6811251.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTTSNAGRFYGI"
BASE COUNT          212 a          146 c          162 g          230 t
ORIGIN      
        1 tggcgtttgt atggcaaagt tattataagt aactatcgct actccattta agttcgctca
       61 gaagaactgg tccacttacg ttagttatta agcaaacgtt cgcttttata aacataatca
      121 ggattaaaat gttggattat tgctaaccca gcacagctag tgcgcgtctg taattataag
      181 ggaaaacgat gaagaataag gctgataaca aaaaaaggaa cttccttacc catagtgaaa
      241 tcgagtcact ccttaaagca gcaaataccg ggcctcatgc agcacgtaat tattgtctga
      301 ctttgctttg ttttattcat ggtttccggg cgagtgaaat ttgtcgattg aggatttcgg
      361 atattgatct taaggcaaag tgtatatata tccatcgatt aaaaaaaggc ttttcaacaa
      421 cgcacccgct attgaacaaa gaagttcagg ctttaaaaaa ctggttgagt atccgtactt
      481 cttatccgca tgctgagagc gagtgggtat ttttatcacg taaggggaat ccgctttctc
      541 ggcaacagtt ttaccatatt atctcgactt ccggtggtaa tgccgggttg tcactggaga
      601 ttcatccgca catgttacgc cattcgtgtg gttttgcttt ggcgaatatg ggaatagata
      661 cgcgacttat ccaggattat cttgggcatc gcaatattcg tcatactgtc tggtatacca
      721 ccagcaatgc agggcgtttt tacggcatct
//
LOCUS       DAZRUF010000524          744 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZRUF010000524 DAZRUF010000000
VERSION     DAZRUF010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.527"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..323)
                     /locus_tag="ACHXOL_004473"
     CDS             complement(<1..323)
                     /locus_tag="ACHXOL_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR6811252.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRI
                     GLRQN"
BASE COUNT          228 a          172 c          164 g          180 t
ORIGIN      
        1 aagttctggc gcagccctat gcgccacagc ggtgggcata ccttcatgct gtacagcatc
       61 tggtttttgt cgttctcccc cagttcaaac caggtcacca cacctttcac ccggcgctgt
      121 acgtcatcgc cctgccatac cgtcaggtag gccattttgt ccagcacctg cgcaaattca
      181 agggagagaa actgctggct gaccagagag agatcgaggg aaaagagcga agagagtgac
      241 tggttcaaat gaaaggaaac caccgcaaaa gcatccggtg gcaggccgtc cacttccagt
      301 gtgaaacgta atccggttga catagcaacc tccgtgaaaa tgaataataa tccgcacata
      361 ttgcatgtac aacatgtcgg gcggatgaga taagcgagtc gacatctggc gttaccgggg
      421 aatatcagaa aaatgtcatg gttatgtgat gatttagtct gggattttac ttcagagaaa
      481 cgggtgtctg tttagtcaac aaaataatca caaacgcaac ggatgggttc tcgacgttat
      541 tcacgacagg ctcgtacatt acagcgagta ataaaaaacc tgatatgcaa agtcaaacca
      601 atattcataa tgtcttttta ttcacaacaa taaataattc aataacaatc attgaaatga
      661 atgatggttt gtataaggaa tatacgctac cgcccaccct gataacatga tctgcacatt
      721 gccatcttaa cgtcgttaaa cact
//
LOCUS       DAZRUF010000525          743 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZRUF010000525 DAZRUF010000000
VERSION     DAZRUF010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.528"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            41..673
                     /locus_tag="ACHXOL_004474"
     CDS             41..673
                     /locus_tag="ACHXOL_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR6811253.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          232 a          126 c          154 g          231 t
ORIGIN      
        1 tgaaattatg caattatata cggataggga ggttcttaac atggggcttt gtagtcgtta
       61 taaaagtctt acatgcaata gttgctcaat gcattgccaa ataatgccag aagagtcacc
      121 gcgtttgcag tattgcgcaa actcgtgttt ttgtatgtgg cccgaagaaa gttcatattt
      181 taatcgtggt gtggtggaag gtatcttaac aaaaaaccac aatgcgagat taagcggata
      241 tatttttgtg gatttttcag taagtttttt acgtctattc cttgaaaaag actggattga
      301 ctatcttgca agtactgaca tggggatagt tttagtcagc gaccgcaata tgcaatcgtt
      361 agctaactac tggcgaaaac acaacaccgc aatatctgct gtcatatata atgatgatgg
      421 tcttgatgtt gccaatgaaa agatcagaca actatttatt ggtcgttatt tatcatttac
      481 cagaggtaac acattaactc agatggaatt caccataatg gggtatatgg tttctggtta
      541 taatccatat cagattgctg aagtgctgga tatggatatc cgtagcatct acgcgtacaa
      601 gcaacgaatc gaaaagagaa tgggtggtaa aataaacgaa ttatttattc gttcacattc
      661 ggtccaacat tgatactcaa cgaccagcca gaatcatact ctgttcatac gggcatctat
      721 tgaaatgccc gtattttata aat
//
LOCUS       DAZRUF010000526          736 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZRUF010000526 DAZRUF010000000
VERSION     DAZRUF010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.529"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /locus_tag="ACHXOL_004475"
                     /pseudo
     CDS             complement(1..736)
                     /locus_tag="ACHXOL_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          195 a          195 c          158 g          188 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccgacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg ataggcaaat ggtgtattgc cagtgacatc ataaccagca
      661 ccgtgcagac caataccata agtatacgca ggcgcatacc agccaaactg cggaccattt
      721 accatgattg ctttcg
//
LOCUS       DAZRUF010000527          729 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZRUF010000527 DAZRUF010000000
VERSION     DAZRUF010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.530"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..443
                     /locus_tag="ACHXOL_004476"
     CDS             <1..443
                     /locus_tag="ACHXOL_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR6811254.1"
                     /translation="VSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSIL
                     DQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGY
                     TANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(483..>729)
                     /locus_tag="ACHXOL_004477"
     CDS             complement(483..>729)
                     /locus_tag="ACHXOL_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR6811255.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
BASE COUNT          190 a          205 c          195 g          139 t
ORIGIN      
        1 ccgtcagcaa aaaccacgca atgatagtca ttgaggattt gaaggtcagc aacatgtcaa
       61 agtcagcagc gggtacggtc agccagccgg ggcgcaatgt ccgggcaaaa tcaggtttaa
      121 accgttcgat actggatcag ggctggtatg aaatgcaccg ccagcttgag tacaagcagc
      181 tctggcgtgg cggtcaggtg cttgctgttc cgccagcgta tacaagccag cgttgcgcgt
      241 actgtggtca tacagcgaaa gagaaccgcc tgtcacaaag taaattcaga tgccaggtat
      301 gtggatatac agcgaacgcc gatgtaaatg gtgctcgtaa cattttagcg gcggggcacg
      361 ccgttcttgc ctgtggagag atggtgcagt caggccgccc gttgaagcag gaacccaccg
      421 aaatgattca ggcgacagcc tgaacgtagc agggatccac gtccttcagg gcgtggagga
      481 tgtcaatatc ggtttctgtc agcgattaaa ttcaacacca cgtaacaaac gcctccggca
      541 attaatcccc agaacgcaga accaatcccg accagcgtca atccacttgc cgttaccaga
      601 aatgccacca ccgccgcgtc gcgctcacgc tcacgctcat tatgcagcgc ctgatacaaa
      661 ctgccgccaa tggtactcaa cagcgccaga cctgccagca tctggatcca acttacgggc
      721 agggcagcc
//
LOCUS       DAZRUF010000528          726 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZRUF010000528 DAZRUF010000000
VERSION     DAZRUF010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.531"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            35..>726
                     /locus_tag="ACHXOL_004478"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHXOL_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFR6811256.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZRUF010000529          725 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZRUF010000529 DAZRUF010000000
VERSION     DAZRUF010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.532"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            133..717
                     /locus_tag="ACHXOL_004479"
     CDS             133..717
                     /locus_tag="ACHXOL_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6811257.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          209 a          149 c          150 g          217 t
ORIGIN      
        1 acaatggttg taaattttgg taacaaatat cattgctcat taatatctag catctgctta
       61 taacatttgt gtcatgaata gtcattcttc aaaccagtag tctgactaat ggaattttat
      121 tcaaggattt ttatgtctaa aaaattaggt tttgccctgt ctggtcttat gctggcaatg
      181 gttgcaggta ctgcttctgc tgatatggat ggtggtcagt taaatatcag tggtctggtt
      241 gttgataaca cctgtgaaac ccgcgttgac ggcggtaata aagatggtct gatcctgctg
      301 caaactgcaa ctgttggtga aatcgatgct ggcgtgctga atgacaccgt tggcgctaaa
      361 gccaaacctt tcagcatcac cgttgactgc agcaaagcaa atccgaatcc aggtagtaca
      421 gctaaaatga ccttcggttc tgttttcttt ggtaatagca aaggaacgct gaacaacgac
      481 atgtctatta acaacccgtc tgacggcgtg aatatcgctc tgcacaatat tgatggttcc
      541 actatcaaac aggttcaaat taacaaccct ggcgatgtgt atactaaagc cctggatgca
      601 acgacaaaat ctgctgttta tgattttaaa gcgtcttacg ttcgtgccgt cgcagaccaa
      661 acagcaactg ctggttatgt aaaaactaac actgcataca ccattactta tcagtaagta
      721 ttcac
//
LOCUS       DAZRUF010000530          709 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZRUF010000530 DAZRUF010000000
VERSION     DAZRUF010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.533"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..250)
                     /locus_tag="ACHXOL_004480"
     CDS             complement(<1..250)
                     /locus_tag="ACHXOL_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR6811258.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRV"
BASE COUNT          184 a          127 c          159 g          239 t
ORIGIN      
        1 aaactctttt aaactgctga gctctttcag tgatattttc tttgtgagga gccataattt
       61 tattagcaga gactttatcg tttgaaaaac aacgataagc tgcctttgtt tcactccagg
      121 tacggcagga aacaggaata ctttcttctg gtgaacgact gaggctattg aggatatgtt
      181 ttactctttt atcaagtctt gagtccccta tatccagtga ttttagctcc tgaataaccc
      241 agttttccat aacacctctg aaataatctc tgtcttttca atataatact attatttcaa
      301 cttgtgggta agggtatgag atgcgaagtg atgatcgata gtgatgcgaa taagtgttcg
      361 attcagatgc gattatgcaa tatgatttta tgtgtgttgc ttaattatca tatttcttgc
      421 ccgttccccg ccgcgcagat ccggtttccg gctgaagtgg atacagacct gatttgttgc
      481 tgagtatcgg taaaagtgcg gcttataagc tattctgaaa catggcaagt gaatctgggc
      541 ggtataattc cggcaaatgt catctgtatt taaaggacgt gtgagtatga aatttctgga
      601 atgtgctgtc cgggatgtga tttatgggat caatgtccgt attgtcaaac cggtcaacat
      661 ttatgaatgt gaactcaggg acaacgtgtt tgtcggcccc ttcgttgag
//
LOCUS       DAZRUF010000531          708 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZRUF010000531 DAZRUF010000000
VERSION     DAZRUF010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.534"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            18..>708
                     /locus_tag="ACHXOL_004481"
     CDS             18..>708
                     /locus_tag="ACHXOL_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR6811259.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKI
                     VVKYNNWITKDN"
BASE COUNT          238 a          126 c          138 g          206 t
ORIGIN      
        1 tgtaagatca aatttatatg gtggcgaaaa ttaatctctt atcacggctt acgccgcttt
       61 tattcgtgtt cgctcctttt ttggcacagt caaatatgac tgtttatcct atggctgttt
      121 ccattaatag tcagggtgaa ggcaacgttc gcgtaatatc taaaagtaat gaagttcagt
      181 acatcaaggc gacagtattc cgtatcgata atccatcgac gcctcaggaa aatgaagttg
      241 agattaaatc cggtgatgca aatcatctgg ttgttatgcc acctaaattc gctttaccag
      301 ccggtagtag caaaactgtg cgctttgttg caatggaacc agagcaaaaa gaaaaaaatt
      361 atcgcgttaa atttgaagcg gttcccagta ttgatgacgt tgccacagat aaaaaagatc
      421 tctctatgca gttaacagtt aacttaattt gggggattgt tgttagtgtt ccacctcagc
      481 aacctattgc taagttagaa gtaaatgctg cccaaaaatt agttaatgca gggaatcaac
      541 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
      601 agaccgtaaa taaaaacatc ttccttggtc aggaaagaaa tcttgaaagc atatctggct
      661 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatg
//
LOCUS       DAZRUF010000532          704 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZRUF010000532 DAZRUF010000000
VERSION     DAZRUF010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.535"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>704)
                     /locus_tag="ACHXOL_004482"
     CDS             complement(<1..>704)
                     /locus_tag="ACHXOL_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR6811260.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVE"
BASE COUNT          124 a          250 c          167 g          163 t
ORIGIN      
        1 actcaacagc ctgattaaaa tcacttggat caccgttaac atggaatttt aaacccaaag
       61 aatctataaa ccgaacaggg tttaatggat atttatacag attccattca cctctcagcc
      121 ctatcggatc ctgggtgata taccttccct gcaacgggtc atagtaccgg ttgcggttgt
      181 agtacagccc cgactcctca tcatactgct gccccggcag acggagtgac tgctggaggt
      241 gctgcgcgct ggtttcgccc agcaggtttc cccattcgtc atactccccc tgccacgccg
      301 tttccccttc cgggctgatg agcgccagcg gcagtccccg gtggtcgcag tggtaaagat
      361 gaagtttcct ctccgggatg tattccgcct ccagctgcgc ggccatctgc tccgccgtca
      421 gcccgcactg cgcaagccat gcctcactct ccgcactcac cgctcccgcg cgcagttccc
      481 gctccagcct ccccagcatc accgacagct ccgccggtag cgtcacaccc gtgtcctcct
      541 gcaacacctc cgccaggctc cggtgccgcg ccttcgcctg ttcgccattc tctgtttcga
      601 ttctcaggag cggcgtgaag ctccccggct ggtataccgt ctggatacgc gtggtgccgg
      661 tctgtatcgt cgtcagcctg tcaccgtccc acccgtacca tgtc
//
LOCUS       DAZRUF010000533          701 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZRUF010000533 DAZRUF010000000
VERSION     DAZRUF010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.536"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            132..>701
                     /locus_tag="ACHXOL_004483"
     CDS             132..>701
                     /locus_tag="ACHXOL_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR6811261.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELD"
BASE COUNT          196 a          159 c          196 g          150 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga c
//
LOCUS       DAZRUF010000534          701 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZRUF010000534 DAZRUF010000000
VERSION     DAZRUF010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.537"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..255
                     /locus_tag="ACHXOL_004484"
     CDS             <1..255
                     /locus_tag="ACHXOL_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFR6811262.1"
                     /translation="LALVISGCVFLYTIFRDNTKDTNDLLTRVATLETTIAVLESSIS
                     RIEDDQDKMRDTLSKLETQIHELDVKIERIIAILQQKQRP"
     gene            complement(294..>701)
                     /locus_tag="ACHXOL_004485"
     CDS             complement(294..>701)
                     /locus_tag="ACHXOL_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFR6811263.1"
                     /translation="KEYQANLKYFRNGKFYPYADSLGCSTIVYGHLIQAGEDFSTGIT
                     ESEADKLLSRDLAKTIMQVQTLGLNLPDDWNDFIIIMTFQLGIGGVKKFRKMLAALKA
                     HDYLEAIKQAKCSLWYRQTPNRVNSMIAVLKNK"
BASE COUNT          214 a          137 c          120 g          230 t
ORIGIN      
        1 ttagctcttg ttatttctgg atgcgtattc ctttatacta tctttagaga taatacaaag
       61 gatacgaatg atctattaac tcgcgttgct actcttgaaa ctaccattgc tgtactggaa
      121 agtagtattt cccgtattga ggatgatcag gataagatga gagatacact aagtaaactg
      181 gaaacacaaa tccatgaact tgatgttaag attgaaagga ttattgcaat tcttcagcag
      241 aaacaacgac cataaaaaaa ggcatagtat gtcattcgta ctatgccttt tctttatttg
      301 tttttaagta ctgcaatcat ggaattcaca cggtttggtg tctgcctgta ccatagagaa
      361 cacttagcct gtttaattgc ttcaaggtaa tcatgtgctt tcagggctgc taacatctta
      421 cggaatttct tcaccccgcc aatacctaac tggaaggtca tgataataat aaaatcattc
      481 cagtcatcgg gaagatttaa ccctaatgtc tgtacctgca taattgtttt ggctaaatcg
      541 cgagatagta acttgtctgc ttcgctttca gtaatgccag tactaaaatc ttctcccgcc
      601 tgaattaagt gaccatatac aatagtacta catcctaaac tatcagcata tggatagaat
      661 ttcccatttc tgaagtactt caaatttgcc tgatactctt t
//
LOCUS       DAZRUF010000535          699 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZRUF010000535 DAZRUF010000000
VERSION     DAZRUF010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.538"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>699
                     /locus_tag="ACHXOL_004486"
     CDS             <1..>699
                     /locus_tag="ACHXOL_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811264.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGI"
BASE COUNT          238 a          138 c          183 g          140 t
ORIGIN      
        1 gattgctcaa cagaaagcta aaacagaaaa gcagaatcaa agagcacaag ctgccgcagc
       61 aagagcacag gcaaggcaac agcaacaaat agcagcacgt aaagagaaag cagaattaaa
      121 actccttgat gtatcaagct ctatcagtgc aatgcaccgt ctctctgtag cggaacaata
      181 caaagctatt gcacaggcta gagagatagc aaaacaatat gagcaagggg ctatcagcct
      241 ggcgcgtatg aatagtcaga tgaaacgcct acaacagcaa caacgtaaga ttaatggcaa
      301 cagaaaagct caatttgccc ccgttaaggg aggatcaggg aattccgcca cgatgggcgc
      361 tctgttattt ggaggcgcta cggctgctgc tggcgtgatg gctgttagta aaacctctga
      421 atttatggct aacagctttg ctaatgctga aacagaaggg gagctaatcc aacgtgcaag
      481 gcttggtgga gttgattaca acttacttaa taatcttgag cgttgggcgt atgacaacgg
      541 gatcgatagc ctccagggtg atccgggacg cagaaaaata ctagatgcca tcggtaaaga
      601 tgtaaaggaa cgcgctacca aaagctatga cgaggctgaa tatgtagttg ataagaaaac
      661 agggaaaggc atatggaagg gcggtgattc tggtattaa
//
LOCUS       DAZRUF010000536          699 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZRUF010000536 DAZRUF010000000
VERSION     DAZRUF010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.539"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>699
                     /locus_tag="ACHXOL_004487"
     CDS             <1..>699
                     /locus_tag="ACHXOL_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR6811265.1"
                     /translation="AMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPE
                     LPVPDFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFS
                     VHRLNKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGK
                     QYLFGQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKW
                     VFHGVTSTVGFLSST"
BASE COUNT          232 a          138 c          142 g          187 t
ORIGIN      
        1 gcaatgctgg atataaataa tatatggacc cgcgattatc ttgaccttgc tcaaaacaga
       61 ggagagttca gaccgggtgc aacaaatgtt caattaatga tgaaagatgg aaagatattt
      121 cattttccag aactacctgt acctgatttt tctgctgttt ccaacaaagg tgcaacaaca
      181 tcaattggag gtgcgtacag tgttactgcg actcataacg gtacacagca tcatgcaata
      241 acaacacagt catgggatca gacagcatat aaagcaagta acagagtatc atctggcgac
      301 ttttcggttc atcgtctgaa taaattcgtc gtggaaacaa caggggttac ggagagtgcc
      361 gacttctcac tttctcccga agatgcgatg aaaagatatg gcgtaaacta caacggtaag
      421 gaacaaataa ttggcttcag agcaggtgcc ggaacaacct caacgatatt aaacggcaaa
      481 caatatctgt ttggacaaaa ctataatccc gacttgttaa gcgcaagtct ttttaatctg
      541 gactggaaaa acaagagtta catttatacc aacagaaccc cttttaaaaa ctcaccaatt
      601 tttggcgata gtggttctgg ttcttatcta tatgataaag aacaacaaaa atgggttttc
      661 catggtgtta ccagtacagt tggttttctc agtagtacc
//
LOCUS       DAZRUF010000537          695 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZRUF010000537 DAZRUF010000000
VERSION     DAZRUF010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.540"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>695
                     /locus_tag="ACHXOL_004488"
     CDS             <1..>695
                     /locus_tag="ACHXOL_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR6811266.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZRUF010000538          691 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZRUF010000538 DAZRUF010000000
VERSION     DAZRUF010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.541"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..469
                     /locus_tag="ACHXOL_004489"
     CDS             <1..469
                     /locus_tag="ACHXOL_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR6811267.1"
                     /translation="YLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYALMHSIS
                     RSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYYKINLS
                     KARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ"
BASE COUNT          230 a          136 c          140 g          185 t
ORIGIN      
        1 ttatttatct ttgagtggag agacacaagg gctaatttca gcaggttgct caacgcttga
       61 ttctgtcgga aacaaagcgc agccagaaca taaagatcag atcatggttt atgcgttaat
      121 gcattccatc tcaagaagtc aaaatgtcaa tcatcatgaa ttaattatca ccaaaccggt
      181 tgataaatcc tcaccattgc tggcaaaagc acttagtgac aatgaaaaaa tggcaatatg
      241 tgaatttata ctctaccgaa ccagtaaagc gggtatttac cagccttatt ataaaataaa
      301 tctgtcaaaa gcccgaatat caagtattga ttttgtcaca ccgcatgctg tcctggaaaa
      361 agaactggaa ccacaggaga gaattgcctt tatttatgaa gatatttcct gggagcatac
      421 gttagccggt acgaatgcaa tgagtaaatg gcaggatcgt gtacagtaac ttactgtaat
      481 aacataataa attactattg cggagagata aagtggctgg tttaaggaat aacaacaaca
      541 cacagaatgc acagtgggct gattatgtgg gtgacattct tcggggggct cagccgataa
      601 accagctagt acctcagcac ccttatctta acgacgtccc actcattgat gagttaaggc
      661 atcagaacac tcatcatgtt attctcttga c
//
LOCUS       DAZRUF010000539          685 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZRUF010000539 DAZRUF010000000
VERSION     DAZRUF010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.542"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
BASE COUNT          192 a          137 c          142 g          214 t
ORIGIN      
        1 gtaagctata tcacttactc aatccatttt acccagagtc ataaaataga gtgtctgttg
       61 atctcatttt catattcctt tcagactgaa gtgcgttttc atcacccgat gagaaagaac
      121 tgaagtgatg tttgccatag attgcgagat gccctgtctc attgactatc gcggtgcacc
      181 tttatacctg ttataccaga tcaaaaatca cgcaagccat acaacaaaac gagatttgca
      241 attcgtgtca caaaatatgt cgatcttttt ctaagaggaa gatgccatgt gaagccagac
      301 gaacacttga ggtggtcttc aaaaactaaa ggatttcttt taactgtttg ttttataaaa
      361 aatttattta gggtctaaac aagggaagct ttgcaagcta actcagtgag cgtggtgaaa
      421 acaagtgttt acccgccatc aggctgatca taattctcac catgaaatat gtttcctgat
      481 ttgtggcttg taactggtcg cttctgaagt cgatctagag aggcttgttg atgttggtgt
      541 tttcaggatg atgtttcact tagtttgttt gccgtatcgc ccggcgaata gctgtgattg
      601 aggaaggttt aagtcgcagt gaccaaagcc atatttaccc acgaatgtag atgaaaaagc
      661 gtctcctgcg ttccccctta tctcc
//
LOCUS       DAZRUF010000540          682 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZRUF010000540 DAZRUF010000000
VERSION     DAZRUF010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.543"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>682
                     /locus_tag="ACHXOL_004490"
     CDS             <1..>682
                     /locus_tag="ACHXOL_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR6811268.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRSFG
                     RYELTIAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTG
                     VHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGRL
                     TEKTDLIPE"
BASE COUNT          157 a          231 c          196 g           98 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 ttcggccgtt atgaactcac catcgcttat acccctgccg ggcagttaca gagccagcac
      361 ctgaacagcc tgctgtctga ccgcgattac acctggaacg acaacggcga actcatccgc
      421 atcagcagcc cgcgccagac ccggagttac agctacagca ccaccggcag gctgaccggc
      481 gttcacacca ccgcagcgaa tctggatatc cgcatcccgt atgccacaga cccggcgggg
      541 aaccgcctgc ccgacccgga gctgcacccg gacagcaccc tcagcatgtg gccggataac
      601 cgtatcgccc gtgacgcgca ctatctttac cggtatgacc atcacggcag actgacagag
      661 aaaaccgacc tcatcccgga ag
//
LOCUS       DAZRUF010000541          678 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZRUF010000541 DAZRUF010000000
VERSION     DAZRUF010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.544"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            3..464
                     /locus_tag="ACHXOL_004491"
     CDS             3..464
                     /locus_tag="ACHXOL_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR6811269.1"
                     /translation="MFSGANLTVEVGSSAGKTVATDFVEVPEVNTFSTSGFESTVISV
                     KTFNNAYDRKLLGTKSIPDISLAVNYLPDNAVHQNLEQLADDQKRCQVKLSYFTDATK
                     KEGFFVIYTCFVSSTTIGGDKDEVVTKTFTLAVDGEAIDSGLITPKPESGE"
BASE COUNT          235 a          114 c          129 g          200 t
ORIGIN      
        1 atattttttc cggtgctaac cttactgtag aagtaggttc aagtgctggt aaaacagttg
       61 ctactgactt tgtagaagta ccagaggtaa acaccttcag tacttcaggc tttgaaagta
      121 ctgtaattag cgtaaaaaca ttcaataatg cttatgatcg taagctattg ggtactaagt
      181 caatcccgga catttctctt gcagttaact acttaccaga taatgcagta catcagaatt
      241 tagaacaact tgcagacgat cagaaacgtt gtcaggtaaa actaagctat tttaccgatg
      301 caactaaaaa agaaggtttc tttgtaattt atacctgctt tgtttcaagt accactatcg
      361 gtggtgataa agatgaagta gtaactaaga ctttcaccct tgcagttgat ggtgaagcaa
      421 ttgattcagg tctgattact ccaaaaccag aatcgggcga ataataataa ataaagggaa
      481 ggtgctcaac tttccctttt ttattggaga tgaaaatgaa tttaaacgaa ttgaagaaaa
      541 aactacaacc agaattaaaa ccacatgaag taatgggtga aatcatcttt attcatcgtc
      601 caaatggtca ggatttccag aaatgcactg atgtggctaa aactctaatc ctatgtgtaa
      661 aagatgaaaa tggtgatc
//
LOCUS       DAZRUF010000542          672 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZRUF010000542 DAZRUF010000000
VERSION     DAZRUF010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.545"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            79..>672
                     /locus_tag="ACHXOL_004492"
     CDS             79..>672
                     /locus_tag="ACHXOL_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFR6811270.1"
                     /translation="MNKKLYLFPDSNIFLQCKDLTQVNFSEITACDEVCIIITRPIQQ
                     EIDRQKGQGNSRLSKKARKAASLFNQVVDSTDMTLVIRERSPRVFLTMDLSLKPCEQL
                     SEQLDYQEADDRFVGIAAGYCADDPDSEVAIITNDSGPRFSAIKHNIICYKVPSSWLL
                     PAEPDEKDKQIKLLEAKLKQFTQSMPDFSIKVADDEQL"
BASE COUNT          210 a          130 c          138 g          194 t
ORIGIN      
        1 ggtaagaatc atgctttgat aaccaattga taactattat tatattaatc taacaccatt
       61 gacatagttg aggtttgaat gaacaaaaag ctgtatttat tcccagactc aaacattttt
      121 ctacaatgca aggacttgac gcaggtaaat ttttccgaga ttactgcttg tgatgaagta
      181 tgtatcatta ttacaaggcc cattcagcaa gagattgacc gccagaaagg gcaaggtaat
      241 tcccgcctat ccaaaaaagc aaggaaggct gctagcctat ttaatcaagt agttgattca
      301 actgacatga ctcttgttat tcgtgaacgc tcgccgcgag tttttcttac catggatcta
      361 agcctgaaac catgtgaaca gttatctgaa caattggatt atcaggaggc cgacgatcgt
      421 tttgttggta ttgcggcagg gtattgcgct gacgatccag attcggaggt ggcaatcatt
      481 accaatgata gcggtccacg tttttcagca ataaaacata atataatctg ctataaagtg
      541 ccttcttcat ggttgcttcc tgctgagcca gatgaaaagg ataaacaaat taaattgctg
      601 gaagctaagc tgaagcaatt cactcagtct atgcccgatt tcagcattaa agtagccgat
      661 gacgagcaat ta
//
LOCUS       DAZRUF010000543          669 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZRUF010000543 DAZRUF010000000
VERSION     DAZRUF010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.546"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>669
                     /locus_tag="ACHXOL_004493"
     CDS             <1..>669
                     /locus_tag="ACHXOL_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFR6811271.1"
                     /translation="RIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQ
                     NALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDRE
                     ALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARM
                     LGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHP
                     HSALG"
BASE COUNT          165 a          161 c          208 g          135 t
ORIGIN      
        1 ccgtatacac catgttatcg gagagctgcc cacgtatggt tatcgtcggg tatgggcgct
       61 gcttcgcaga caggcagaac ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg
      121 gatcatgcgc cagaatgcgc tgttgcttga gcgaaaacct gctgtaccgc catcgaaacg
      181 ggcacataca ggcagagtgg ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt
      241 cgagttctgc tgtgataacg gagagagact gcgtgtcacg ttcgcgctgg actgctgtga
      301 tcgtgaggca ctgcactggg cggtgactac cggcggcttc aacagtgaaa cagtacagga
      361 cgtcatgctg ggagcggtgg aacgccgctt cggcaacgat cttccgtcgt ctccagtgga
      421 gtggctgacg gataatggtt catgctaccg ggctaatgaa acacgccagt tcgcccggat
      481 gttgggactt gaaccgaaga acacggcggt gcggagtccg gagagtaacg gaatagcaga
      541 gagcttcgtg aaaacgataa agcgtgacta catcagtatc atgcccaaac cagacgggtt
      601 aacggcagca aagaaccttg cagaggcgtt cgagcattat aacgaatggc atccgcatag
      661 tgcgctggg
//
LOCUS       DAZRUF010000544          664 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZRUF010000544 DAZRUF010000000
VERSION     DAZRUF010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.547"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
BASE COUNT          209 a          159 c          130 g          166 t
ORIGIN      
        1 cagacctgtg ctatcggaaa gaaaaaaggc catcagcaaa actctgatgg cctgaatcac
       61 ctttaccaat attgtatgaa aaaacacgca cgactaattg acaataattt tcatttccat
      121 taaaaaagat cgcgtagcat tcttaattca tgaggaactt tacccgccag caatctgagt
      181 agcgaaagct gttcatcccc aatgtttttg ctggcgggtc cttttttcta cggtcctcct
      241 ccccggaggg gggcatttca ttatttttct aatcgttact ggtgagaagg cggaagaaaa
      301 agccagttct tcgcccctaa ataacagcat gattccagtc catgcaaaat ctgatttcca
      361 gctcacacat accgagcgct gtacaaaaaa ccgccagcca aggcagttaa gagtgtgttc
      421 ccggggtttg cttagaatat tttgtaagtt gtccgaagag atatttacaa caccagaatg
      481 atgaatcatc agtcccctgc cagaggttca ctacgtaacc tgaccgacaa tgcaccattc
      541 tggtgctgaa aaaagagcac tggaactgca acaaaacaac atcacagaac aggagatggt
      601 aaggagtcag acattcccac acaatattgt gtcagtgcaa ataacaacct tcgtctcaaa
      661 tctc
//
LOCUS       DAZRUF010000545          664 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZRUF010000545 DAZRUF010000000
VERSION     DAZRUF010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.548"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..208)
                     /locus_tag="ACHXOL_004494"
     CDS             complement(<1..208)
                     /locus_tag="ACHXOL_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811272.1"
                     /translation="MDCRKLNIVLLSTVAVLLVVVILVLIKSLFVGSEGFEWGSVSDW
                     VSSIANLIMAGAALYAAWNAKNWFQ"
BASE COUNT          199 a          165 c          113 g          187 t
ORIGIN      
        1 tttgaaacca atttttagcg ttccatgctg catatagggc tgctccagcc ataatgagat
       61 tggcaataga actaacccag tcactaaccg atccccactc gaaaccttca gaaccaacaa
      121 agagtgattt tattagtact aaaattacaa caaccagtaa tactgctacg gtcgataaaa
      181 gaacgatatt taattttcta caatccatcg aaagtagttc ctgaacgcat taaaggggca
      241 aatactttac catccgaaac acaatcaagt acactacccg ttcacgtact gctcacatgt
      301 tgggtttgtt attttgttgg ggtgagcagt acgtgtatcc tcagaagggt agttccccct
      361 gatcacagaa ccaatcacct tcttcactgc tttgattcat ccggtactca gcagatagat
      421 atgctaaccc atcaataaca cttattagtg gtgtttcaca ggtaagtaca aaacaatctc
      481 tctctatagt acctaaccag tattccctgt actgattctg gaagaatacc ctatccccat
      541 aatgaaattc tgttagtgca gatccccaat aacacacggt tgcccagcga ccgttatgtg
      601 agaacttctg aacccctcgc gtaatggatg gatcatagcc gtctttgctc atttgttacc
      661 ctcc
//
LOCUS       DAZRUF010000546          659 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZRUF010000546 DAZRUF010000000
VERSION     DAZRUF010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.549"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            67..606
                     /locus_tag="ACHXOL_004495"
     CDS             67..606
                     /locus_tag="ACHXOL_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HFR6811273.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTV"
BASE COUNT          173 a          122 c          166 g          198 t
ORIGIN      
        1 tataaaaata ttttttgttt cagatgtcat atattttatt aactgaatga ttaacaggag
       61 aatcagatga actggcatta tgaaaaaaat ggtgtacgtt atgataacgt aacagaggaa
      121 gatattatcg aacgtatccg gcgtggtgaa ctgaatgcat caacactggt gtggcaacag
      181 ggcatgctgg aatggcagcc agtttcagcc acatcactgg cggaaatact gaaacaaagt
      241 gaagttcccc cagcccttcc gggtcaccgc attcccggag gcgttgtctg gacgctggca
      301 tttgcccctt tgattgggta catacttgag atgtggactg cagggctgaa cggtatgtca
      361 tttgaacggg cttatgatgc agtcagcgaa ggccagtact ggtttatctc tctgattctg
      421 aatattgctc tgggatatct ggatgaacga aaactccgta aatctggtgt tgatactgca
      481 acatttggct ggctggcatg gttgatcccg gtttatctgt ggcgtcgggc aaaaatcctc
      541 ggacaaaaac ctgcgtattt ctgggtctgg ttggttatgc tgatactggt gttatttacg
      601 gtctgatatt ctttatcgca gagtttacta aataatcagg ttattagtgt tcttaacag
//
LOCUS       DAZRUF010000547          658 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZRUF010000547 DAZRUF010000000
VERSION     DAZRUF010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.550"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>658
                     /locus_tag="ACHXOL_004496"
     CDS             <1..>658
                     /locus_tag="ACHXOL_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR6811274.1"
                     /translation="SQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEI
                     IFKDEFAAVIPDNVSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLTL
                     LRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDK
                     TVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAH
                     G"
BASE COUNT          202 a          129 c          133 g          194 t
ORIGIN      
        1 gtcacagttt tggtcgtacg cgacatactt cagggtattg tcctttgaat atctgagtga
       61 aagtatttcc acactgctgt atctggatgc cgatgtcgtt tgtaaaggaa gtctgaagcc
      121 tctcacagaa attatattta aagatgagtt tgctgcggtc attcctgaca atgttagtac
      181 tcaggcggca tgtgcaaaac gcctcaacat tcccgagatg aatggacgtt atttcaatgc
      241 aggcgttatc tatgtcaatc ttaaaaaatg gcatgaagca aatttgacac cgtatttact
      301 cacgctttta cgaggggaaa ctaaatatgg ctctcttaaa tatttagatc aggatgcgtt
      361 gaatatcgca tttaatatga ataatatcta cctcgcgaag gattttgata ctatttatac
      421 cctgaaaaac gaacttcatg atcgtagtca tcgaaagtat cagcaaacca ttaccgataa
      481 aacagtattg attcactata cagggataac taaaccatgg catagctggg ctggttatcc
      541 gtctgcatca tactttaata tcgcgcgtga acaatctccc tggaagaaat atcctcttaa
      601 agaggcgcgg actgttgcag aaatgcagaa acaatataag catctgtttg cccatggt
//
LOCUS       DAZRUF010000548          651 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZRUF010000548 DAZRUF010000000
VERSION     DAZRUF010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.551"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>651
                     /locus_tag="ACHXOL_004497"
     CDS             <1..>651
                     /locus_tag="ACHXOL_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR6811275.1"
                     /translation="ILDTVRLLQLNNQRLSNGNYISNGIEFDKWHKPVNYYFCKFDPV
                     TYTYITGDYEVIPAAEICHYFIADQQGQERGLPDMVATSKLMEDLKNFTEAALTAKRV
                     SASSMAFITNNNDTTSTDLLGADEREEVTPVYTEYFEAGFIGELAEGQDIKTVTPTNG
                     VDGIDQFTNELMNQISMGLNITKQALLSDTSNASFSASRLTEKLQQTTFRTRTNVLI"
BASE COUNT          203 a          120 c          128 g          200 t
ORIGIN      
        1 attctcgata cggttcgttt gcttcagtta aataatcaac gtttgagtaa tggtaattat
       61 attagtaatg gtattgaatt tgataaatgg cataagccag ttaattacta tttctgtaaa
      121 ttcgatcctg ttacctatac ttatatcact ggcgattatg aagtaattcc cgctgcggaa
      181 atctgtcact actttattgc tgatcaacaa ggccaggaac gcggattgcc ggacatggtt
      241 gcaacttcca aattaatgga agatctcaag aacttcactg aggctgcgtt aactgcgaaa
      301 cgtgtatctg catcgtcaat ggcttttatt accaataata acgacactac cagtactgat
      361 ctgttagggg ctgatgaacg agaagaagtt acaccagtat atacagagta ttttgaagct
      421 ggctttattg gtgagttagc cgaagggcag gatattaaaa ctgttacacc tactaatggt
      481 gttgatggga ttgatcaatt taccaatgaa ttgatgaatc agatctcgat gggtctgaac
      541 ataacaaagc aagccttatt atctgataca tccaatgctt catttagtgc aagccgactt
      601 actgagaaat tacaacaaac tacattccgt acccgtacta atgtactaat c
//
LOCUS       DAZRUF010000549          649 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZRUF010000549 DAZRUF010000000
VERSION     DAZRUF010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.552"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..194
                     /locus_tag="ACHXOL_004498"
     CDS             <1..194
                     /locus_tag="ACHXOL_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR6811276.1"
                     /translation="VDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNL
                     HYIDTGAVFGGELTLVQLQ"
     gene            complement(195..386)
                     /locus_tag="ACHXOL_004499"
     CDS             complement(195..386)
                     /locus_tag="ACHXOL_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR6811277.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(491..>649)
                     /locus_tag="ACHXOL_004500"
     CDS             complement(491..>649)
                     /locus_tag="ACHXOL_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR6811278.1"
                     /translation="LTIPCKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQS
                     DAWIMRLA"
BASE COUNT          184 a          147 c          160 g          158 t
ORIGIN      
        1 acgttgattt gcatcaggtc ttgtggagcc gctcgcgatt aggtgaacgc caaaaagggc
       61 agggaattac aggtgctgat catttctggt ttggtcatac accgttgcga catcgcgtgg
      121 atattggcaa cctgcattat attgataccg gtgctgtctt tgggggcgaa ctgactcttg
      181 tgcaattgca ataattaaaa atcaccgtac tcctgtgcag gtcgccagaa accatctata
      241 aaatcctcaa tcggaaaaca accgccatgg cggatccgtt gatcgctcat agaataaaga
      301 cactgctgtt ccgtgttgta gacatccaca acaatatctt cacaaccgcc atccaggtag
      361 caaacaaaaa gtaccagcgc gaacatttca tccccgaagt gtggtgccgt accgttaagt
      421 ttaggagaga ttttacaacg ggggaataac caggacaaat aacccgccat cccgacgggt
      481 tctttttgga tcaggcaaga cgcataatcc aggcatcaga ttgttgatcg taacgggtac
      541 ggtaaaggac agagtgttcg ccatttagca gggcagggat actggtttca gcatcccagg
      601 catcgagttg catacataaa tcggggtcag atttacaagg aatggttaa
//
LOCUS       DAZRUF010000550          649 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZRUF010000550 DAZRUF010000000
VERSION     DAZRUF010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.553"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(26..330)
                     /locus_tag="ACHXOL_004501"
                     /pseudo
     CDS             complement(26..330)
                     /locus_tag="ACHXOL_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            384..587
                     /locus_tag="ACHXOL_004502"
                     /pseudo
     CDS             384..587
                     /locus_tag="ACHXOL_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          158 a          175 c          153 g          163 t
ORIGIN      
        1 gcatcatttt ttccagaggc ggtcaaactc cgccttcgca aaataagcgg aagcctggcg
       61 aaggatatcg ttactgcggc gcagttcacg attttcacgc tccagctctt tcagacgctg
      121 acgttcagcg gtggtgagcc caccatcacc gcccccggta tcccgctcat gctggcgaac
      181 ccagacacgc agagtctccg gcgtacagcc aatctttggg gcaatggaac aaattgccgc
      241 ccactgtgag tcatattcat cctgactttc cagaaccata cgaatcgccc gctgacggac
      301 ttcgggggaa aaacgagtat ttttagtcat cctgtttacc tctttctcag ggagtttagt
      361 ctccaggatt tccggggcgg ttcagacatc tagaatttac tgtcagcacc ggcgttaaag
      421 tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaat acaaatgggc
      481 taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa catcgagtag
      541 gcagcctgac ggctgcggct tgtcatggtc tgcaattacc gttataaaaa caggcaatgt
      601 cattgtcttt caggtggtta tatggcccgt tcagctaaac cccgtaaac
//
LOCUS       DAZRUF010000551          649 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZRUF010000551 DAZRUF010000000
VERSION     DAZRUF010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.554"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..363)
                     /locus_tag="ACHXOL_004503"
     CDS             complement(<1..363)
                     /locus_tag="ACHXOL_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HFR6811279.1"
                     /translation="MGKSLGGFFGAIITAVVVAAAVYFSGGTALAAIGWGAAAGSLSL
                     VASSMLGQIGVSGYGDVADSLSRSTSPTTGLPVIYGGELPHKNGVSGGSFILTGSIVA
                     WYNVPNSDSQYLFSEQAVA"
     gene            complement(356..>649)
                     /locus_tag="ACHXOL_004504"
     CDS             complement(356..>649)
                     /locus_tag="ACHXOL_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR6811280.1"
                     /translation="INGTTQLTNRQYSSIKEGIAGLNKEGWNHTGEIVEAYCKPVQAT
                     IDGDIWLDPDNPLIMAVVASGRVLGVNADHDGFELQPKPTEGTYYRVRKQRDG"
BASE COUNT          171 a          164 c          119 g          195 t
ORIGIN      
        1 tgctacagcc tgttctgaga ataagtactg agaatcagaa tttggtacgt tgtaccaggc
       61 tactatgctt ccggttagga tgaaggaacc gcctgataca ccatttttat gtggtagttc
      121 tccaccgtat ataacgggta atccagtagt tggtgaagta cttctactta gtgagtctgc
      181 tacatcaccg tatccagaaa ccccgatttg acctaacata gaacttgcaa ctaatgacaa
      241 agatccggct gctgcacccc aacctattgc tgctaatgct gtacctcctg aaaagtacac
      301 agcagccgcg acgacaactg ccgtaattat ggctccaaaa aagcccccta aactcttacc
      361 catctctctg tttccttact ctgtaatatg ttccctctgt tggttttggt tgaagttcga
      421 aaccatcatg atcagcattt actccaagta cccgaccaga tgccactact gccataatta
      481 atgggttgtc aggatctaac cagatatcac cgtctatagt ggcttgtact ggcttacagt
      541 acgcttcaac aatttcccct gtatggttcc atccttcctt gtttaaacct gctatacctt
      601 ctttaatgct cgaatactgg cgattagtta actgtgtagt accattaat
//
LOCUS       DAZRUF010000552          646 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZRUF010000552 DAZRUF010000000
VERSION     DAZRUF010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.555"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHXOL_004505"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHXOL_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR6811281.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
BASE COUNT          142 a          137 c          163 g          204 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attaca
//
LOCUS       DAZRUF010000553          642 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZRUF010000553 DAZRUF010000000
VERSION     DAZRUF010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.556"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>642
                     /gene="fliD"
                     /locus_tag="ACHXOL_004506"
     CDS             <1..>642
                     /gene="fliD"
                     /locus_tag="ACHXOL_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR6811282.1"
                     /translation="KVSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDE
                     SSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLV
                     LTASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSS
                     NKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          218 a          146 c          139 g          139 t
ORIGIN      
        1 aaagtttcca ccgaagcagg tgctgccccc ggtatctata ccatcagcgt aacccaactg
       61 gcgcaggcgc aatctttacg aactgattcc ccgacaatca ttgcatcaac aaaagatgca
      121 ctgggtgatg agtcatcaga tacacgcaca ataaaaatta cccaagacgg tcgtaaagag
      181 ccgctggaaa tcaagctcaa taaagatcag acgtcattgg atgagatcag caaagccatt
      241 aacgatgccg atagtggaat ttccgctagt atcgttaaag tcaaagatgg taactatcaa
      301 ttggtattga ccgccagtga aggtctggcc aataaaatga ccatatctgt tgagggtgac
      361 agtaaattaa atgatctgct ggcttatgac agcaagacca acaccgggaa tatgaaggaa
      421 ctggttaacg cgcaaaatgc acagctaaac gttaacggta tcgatattga acgttcaagt
      481 aacaaaatta ccgatgctcc tcagggagtg actctcgatt tgaccaaaaa agtcactgac
      541 gtccgggtca cggtgacaaa aagcaacgat aaagcaaccg aagcaattaa aggctgggtg
      601 gattcatata actccctcat tgacaccttt aacaccctga ca
//
LOCUS       DAZRUF010000554          640 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZRUF010000554 DAZRUF010000000
VERSION     DAZRUF010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.557"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(1..607)
                     /locus_tag="ACHXOL_004507"
                     /pseudo
     CDS             complement(1..607)
                     /locus_tag="ACHXOL_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
BASE COUNT          190 a          160 c          145 g          145 t
ORIGIN      
        1 tactaccaat ccgattctgt acactatttc cgctgaaatt ataattgagc aagccggcat
       61 taataccggg atcccacaac tcaggaggaa tatatccacg cgcgcgatta ctcataaatg
      121 cctgagggat cgtcaggttg agtcgctgct gaccaacatc taaatgcgca gtagcgtcat
      181 ggatcattga ggttaatggc acgcaggcat catccaccag cagattcata ccggagacag
      241 aagccgtatt cagccccata ctggcaagct gcgcgcgtgt caggcaggga acaatccctt
      301 gttcactgtc gcccgtgtta aatgtgacat cacgcgttgc catataacca ttattcaaat
      361 agatatcgac gcgatacgtc cctggcggta attcttgccc attttcaaaa cgcgataaat
      421 cggccacagc ctagggatca tccgctaaaa agcgcggatt aaaatagagt tcggcagatg
      481 acaaaggtgc ctgtgcggca aaagcacagg cgacaaagag ccggacaaaa agaccagcca
      541 aacgatgctt acgaatatgc aagcattgtg tgtttcgctg gtaaagtctt aaattcagat
      601 atgacattat tcctccggtt taaaatcacc cggcagtcaa
//
LOCUS       DAZRUF010000555          637 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZRUF010000555 DAZRUF010000000
VERSION     DAZRUF010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.558"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>637
                     /locus_tag="ACHXOL_004508"
     rRNA            <1..>637
                     /locus_tag="ACHXOL_004508"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          152 a          143 c          209 g          133 t
ORIGIN      
        1 tcctcctaaa gagtaacgga ggagcacgaa ggttggctaa tcctggtcgg acatcaggag
       61 gttagtgcaa tggcataagc cagcttgact gcgagcgtga cggcgcgagc aggtgcgaaa
      121 gcaggtcata gtgatccggt ggttctgaat ggaagggcca tcgctcaacg gataaaaggt
      181 actccgggga taacaggctg ataccgccca agagttcata tcgacggcgg tgtttggcac
      241 ctcgatgtcg gctcatcaca tcctggggct gaagtaggtc ccaagggtat ggctgttcgc
      301 catttaaagt ggtacgcgag ctgggtttag aacgtcgtga gacagttcgg tccctatctg
      361 ccgtgggcgc tggagaactg aggggggctg ctcctagtac gagaggaccg gagtggacgc
      421 atcactggtg ttcgggttgt catgccaatg gcactgcccg gtagctaaat gcggaagaga
      481 taagtgctga aagcatctaa gcacgaaact tgccccgaga tgagttctcc ctgacccttt
      541 aagggtcctg aaggaacgtt gaagacgacg acgttgatag gccgggtgtg taagcgcagc
      601 gatgcgttga gctaaccggt actaatgaac cgtgagg
//
LOCUS       DAZRUF010000556          635 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZRUF010000556 DAZRUF010000000
VERSION     DAZRUF010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.559"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            164..>635
                     /locus_tag="ACHXOL_004509"
     CDS             164..>635
                     /locus_tag="ACHXOL_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR6811283.1"
                     /translation="MFWNKKNKNSETKPKKIKQERSLKQSTLKRDLQAVRNTSVMNFG
                     FNSNSGSNINFLLMKALPTFRAFSRDAVLKNPIGRKYMNLSVDGVVGSDGLYVKPSVE
                     IDGTEEEINGINLTLEKLFDRWAYDADRFSVDGSLSFDLFQQNAEKVRVQDGECF"
BASE COUNT          204 a          121 c          138 g          172 t
ORIGIN      
        1 ctcttgtaag tgaatcatta tcatcgcttg aatcaatgcg ggaacgctat gtaaaacgtg
       61 ctaatgctct atttgtgaaa atgaatggcg gtactttctc taatggcggt aaaccaatca
      121 agagtattac ggttttcagg cctaagccag ggagtacacg ctaatgttct ggaataagaa
      181 aaataaaaat tcagaaacta aacccaagaa gattaaacag gaacgttcct taaaacaaag
      241 taccttaaaa cgagatctac aggcagtacg caacacatca gtaatgaact tcggattcaa
      301 ttcaaactca ggcagcaata tcaacttcct tctgatgaaa gcactaccta ccttcagggc
      361 tttctcacgt gatgcagtac ttaagaatcc tattggtcgt aagtacatga acctatcggt
      421 agatggcgtt gtaggctctg atggcctgta cgttaaacct tcagtagaga ttgacggtac
      481 tgaagaagaa atcaacggaa tcaatctaac cttggaaaag ctctttgacc gttgggcata
      541 tgatgctgat cgttttagtg ttgatggctc attaagtttt gacctgtttc agcagaacgc
      601 agagaaggta agggtccagg atggtgaatg cttta
//
LOCUS       DAZRUF010000557          632 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZRUF010000557 DAZRUF010000000
VERSION     DAZRUF010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.560"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..566)
                     /locus_tag="ACHXOL_004510"
     CDS             complement(<1..566)
                     /locus_tag="ACHXOL_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFR6811284.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIV"
BASE COUNT          254 a          141 c           89 g          148 t
ORIGIN      
        1 acaatacaaa ctattgtcat cagaaccaca gatataataa cgctacttga actattggca
       61 tgtagcatct ttgtcgagat aaagaaaaca gtccccatcc caagggccat taaaatcggc
      121 aagaaaaagg tcacccacca gaaccctttc atagagcaat taaaactaaa tcttacgccg
      181 ttgagactgg tcatcaaggc ctgataacgc agtccttttg ctgccataaa tataagcaaa
      241 accaacagtg acaaagtcaa aacacagcca ataagcggca tatctgctga cactgtcatt
      301 aagattgcga aatagaaaaa aacaaaaaca agacaactaa caaaaacatt cccaccggta
      361 atcccataag aaaatcgttg tccgttaact tccatattag catagagata acgcttacat
      421 ttcattaatg cccatggtaa atagatacct agcgttataa tcgttaacaa cacattcacc
      481 acacaaataa gaaaatatct ctcacctgta ccatgaaaaa caaacgaatg ggacggaaca
      541 tccatatcat taataacttg agccataaaa gttccctgaa ttaaaaaatc cattgatata
      601 atttatatat acagacagta aaatgttctg cc
//
LOCUS       DAZRUF010000558          624 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZRUF010000558 DAZRUF010000000
VERSION     DAZRUF010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.561"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..80)
                     /locus_tag="ACHXOL_004511"
     CDS             complement(<1..80)
                     /locus_tag="ACHXOL_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811285.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNAS"
     gene            complement(90..>624)
                     /locus_tag="ACHXOL_004512"
     CDS             complement(90..>624)
                     /locus_tag="ACHXOL_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811286.1"
                     /translation="LGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGALSE
                     SIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLASF
                     ACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDKWK
                     RENMTPFMYVLNTPPVI"
BASE COUNT          204 a          132 c          107 g          181 t
ORIGIN      
        1 gaagcattga atgcagggaa aaataatatg gccataaaaa acatcgaaag aaactctttt
       61 aatttaacat gtaaacgcat ggttaatcct catatcacgg gtggagtgtt aagaacatac
      121 ataaatggag tcatgttttc ccttttccat ttatcaagtt cctgttgccg ttttagtcca
      181 tctctaattg catattttaa tttttctgat aaatggcatt gagcatcgat ttcatttaaa
      241 acaactgtac acactatccc tgtcagtaaa attgctcctc cgacaagaat tactggaaag
      301 ccaaagctaa cacaggcaaa agaagccagg cttccaatag cggttgcaac accaccggct
      361 ataactgctt tggccacatc cattgaaaaa tcgccaagaa aattaaccag atctcgctct
      421 gaactcataa taaattctat ggttctccag gctactgaaa accagataca gaacttaact
      481 ccttttattg attcagataa agctcctcgc cagccaatag cgagttccag aacttgaggc
      541 gctcgtgaag agaaaatata tttaccgagc ttttctaaaa gccaggggct ggatgttgtg
      601 atttttatat atttctctcc aagt
//
LOCUS       DAZRUF010000559          623 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZRUF010000559 DAZRUF010000000
VERSION     DAZRUF010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.562"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>623
                     /locus_tag="ACHXOL_004513"
     CDS             <1..>623
                     /locus_tag="ACHXOL_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR6811287.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQV"
BASE COUNT          126 a          179 c          178 g          140 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggt
//
LOCUS       DAZRUF010000560          608 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZRUF010000560 DAZRUF010000000
VERSION     DAZRUF010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.563"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>608)
                     /locus_tag="ACHXOL_004514"
     CDS             complement(<1..>608)
                     /locus_tag="ACHXOL_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR6811288.1"
                     /translation="VIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKD
                     AVTNNIALMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVH
                     YQDLLTRFSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLP
                     VPVATMVREDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNI"
BASE COUNT          201 a          117 c           88 g          202 t
ORIGIN      
        1 cttatgtttt gctttattaa tgccacgtca ttaaaatgac tatatttaat gaaaaaacca
       61 tcattgagta gtgttttaag attgactaaa ttacttgcat cttcacgtac catcgtggca
      121 actggaacag gcagagacat gaaaacttct tcattcacca tcaagtgaat catgcattgc
      181 gttccctttg tcatatccac acatacgcta caaatatcgt ggtaatgcga agagcgcatt
      241 tcaggaaaca gattaaacac ggtatcttga aaggcactaa atcgtgttaa taaatcctga
      301 taatgaacag gttttgtttc aaaattatca tcagcaaatc caagcactat tgtatgctct
      361 acatctaacg cagtttcaaa tggctcttct tctgtaaaat cattatttaa aacctcacct
      421 gacgtattat cactgtcata aaaagtgttc atcaatgcga tattatttgt tacagcgtct
      481 ttttcgatat tctcactaat tgcatatcgc ctgctataat cataaaaatg aataaacgcg
      541 ccctttataa aggatatata ttgttctttg ttaaagtcaa tacggctgta taatttttca
      601 aaaatcac
//
LOCUS       DAZRUF010000561          600 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZRUF010000561 DAZRUF010000000
VERSION     DAZRUF010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.564"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(261..>600)
                     /locus_tag="ACHXOL_004515"
     CDS             complement(261..>600)
                     /locus_tag="ACHXOL_004515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811289.1"
                     /translation="LMARLREVLNAIFSAPRSGKSRIPKYQQGIFLRNQHRFYLLNHY
                     TESLSVMSLTKLSLNTRMIGFMSDQMLETAPRLTRAVSDETSVYAGAGQNIGQNPLNI
                     INVICAKEHI"
BASE COUNT          186 a          125 c          103 g          186 t
ORIGIN      
        1 tagcaagccc ttttcttgtg ttgtcaagac atttggcaac gttcaaacat ctgtttagaa
       61 atgccaaatt gttgatctaa caaataaacc tgtataaaca gaacaccatt taatctattc
      121 ccctgtgtaa cccaccacat cacacaataa aaacaaaaaa ctttaattat atttcatagc
      181 atacaaaagt caatagaagc cctttcgtgc tcctacgtaa aaaatccatc acttttcccc
      241 tgatacatca attcaaggcg ttatatgtgt tccttagcac atataacatt tattatgtta
      301 agtggatttt ggcctatatt ttgacctgca cccgcataaa cactggtttc atcgcttaca
      361 gcccttgtga gacgtggtgc tgtttctagc atctgatccg acataaaacc gatcattctc
      421 gtgtttagtg aaagtttagt gagagacatt acagaaaggc tttctgtgta atgatttaat
      481 aggtaaaaac gatgttgatt acgcaagaaa attccttgct gatatttggg gattctggat
      541 tttccagatc ggggggcgct gaaaatcgcg ttaagcacct ctcgcaatct agccataagg
//
LOCUS       DAZRUF010000562          597 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZRUF010000562 DAZRUF010000000
VERSION     DAZRUF010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.565"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            26..>597
                     /locus_tag="ACHXOL_004516"
     CDS             26..>597
                     /locus_tag="ACHXOL_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR6811290.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDP"
BASE COUNT          161 a          143 c          151 g          142 t
ORIGIN      
        1 atccaatcat tttaaggagt ttaaaatgga taagaagcaa gtaacggatt taaggtcgga
       61 actactcgat tcacgttttg gtgcgaagtc tatttccact atcgcagaat caaaacgttt
      121 tccgctgcac gaaatgcgcg acgatgtcgc attccagatt atcaatgacg aattatatct
      181 tgatggcaac gctcgtcaga acctggccac tttctgccag acctgggacg acgaaaatgt
      241 ccacaaattg atggatttat ccattaacaa aaactggatc gacaaagaag aatatccgca
      301 atccgcagcc atcgacctgc gttgcgtaaa tatggttgcc gatctgtggc atgcgcctgc
      361 gccgaaaaat ggtcaggccg ttggcaccaa caccattggt tcttccgagg cctgtatgct
      421 cggcgggatg gcgatgaaat ggcgttggcg caagcgtatg gaagctgcag gcaaaccaac
      481 ggataaacca aacctggtgt gcggtccggt acaaatctgc tggcataaat tcgcccgcta
      541 ctgggatgtg gagctgcgtg agatccctat gcgccccggt cagttgttta tggaccc
//
LOCUS       DAZRUF010000563          597 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZRUF010000563 DAZRUF010000000
VERSION     DAZRUF010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.566"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            70..>597
                     /gene="dosC"
                     /locus_tag="ACHXOL_004517"
     CDS             70..>597
                     /gene="dosC"
                     /locus_tag="ACHXOL_004517"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR6811291.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLE"
BASE COUNT          160 a          122 c          147 g          168 t
ORIGIN      
        1 gcagttagtc aatctcgcac gttgcgtttt gattccgtct gatttcccag ccttataagg
       61 gtgtgaatta tggagatgta ttttaaaaga atgaaagatg agtggaccgg acttgtcgaa
      121 caggcagatc cactcattcg tgctaaagcc gcggaaattg ccgttgcgca tgctcattat
      181 ctgagtatcg agttttatcg aattgtccgc atcgacccgc atgccgaaga attcttgagt
      241 aatgaacaag ttgagcggca gttgaaaagt gcgatggaac gctggattat taacgtgctt
      301 tctgcccagg ttgacgatgt cgaaaggcta atacaaatcc agcataccgt cgcggaagtg
      361 catgcccgca taggaattcc ggtagaaatt gtcgagatgg ggtttcgggt gctgaaaaaa
      421 atcctctatc cggtaatctt ctcttcggat tattccgccg cagaaaaact tcaggtctat
      481 catttctcga ttaacagtat tgatatcgcg atggaagtga tgacccgcgc gtttaccttt
      541 agtgacagta gtgcctcaaa ggaagatgaa aattatcgta tcttctcgtt actggaa
//
LOCUS       DAZRUF010000564          597 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZRUF010000564 DAZRUF010000000
VERSION     DAZRUF010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.567"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            39..>597
                     /locus_tag="ACHXOL_004518"
     CDS             39..>597
                     /locus_tag="ACHXOL_004518"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFR6811292.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFI"
BASE COUNT          175 a          137 c          140 g          145 t
ORIGIN      
        1 tggcgtaggg aaaatcacat ctgaatcagg aattaacaat gaaacctgta aaaccacctc
       61 gtattaatgg acgagtgccg gtcctgtcgg cacaggaagc ggtgaattat attcccgacg
      121 aagcaacact ttgtgtgtta ggcgctggcg gcggtattct ggaagccacc acgttaatta
      181 cagctcttgc tgataaatat aaacagactc aaacgccacg taatttatcg attattagtc
      241 cgacagggct tggcgatcgc gccgaccgtg gtattagtcc tctggcgcaa gaaggtctgg
      301 tgaaatgggc attatgtggt cactggggac aatcgccgcg tatttctgaa ctcgcagaac
      361 aaaataaaat tattgcttat aactatccac aaggtgtact tacacaaacc ttacgcgccg
      421 ccgcagccca ccagcctggt attattagtg atattggcat cgggacattt gtcgatccac
      481 gccagcaagg cggcaaactg aatgaagtca cgaaagaaga cctgattaaa ctggtcgagt
      541 ttgataacaa agaatatctc tattacaaag cgattgcgcc agatatcgcc ttcattc
//
LOCUS       DAZRUF010000565          593 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZRUF010000565 DAZRUF010000000
VERSION     DAZRUF010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.568"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHXOL_004519"
     CDS             <1..91
                     /locus_tag="ACHXOL_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811293.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="ACHXOL_004520"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="ACHXOL_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR6811294.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAZRUF010000566          592 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZRUF010000566 DAZRUF010000000
VERSION     DAZRUF010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.569"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            140..>592
                     /locus_tag="ACHXOL_004521"
     CDS             140..>592
                     /locus_tag="ACHXOL_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR6811295.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRN"
BASE COUNT          228 a          125 c           78 g          161 t
ORIGIN      
        1 agcgcaaaat aacaaactga caaaaattaa tatttctgat agttataaac tgaaatttct
       61 taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat ctctgattga
      121 gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc ccaggatgaa
      181 aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca ctacaaaact
      241 ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg atcaactaat
      301 atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc cacttgcaca
      361 aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag tgaccattga
      421 tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca catctatcaa
      481 tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac tcgtaaagct
      541 aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa at
//
LOCUS       DAZRUF010000567          589 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZRUF010000567 DAZRUF010000000
VERSION     DAZRUF010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.570"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            108..257
                     /locus_tag="ACHXOL_004522"
                     /pseudo
     CDS             108..257
                     /locus_tag="ACHXOL_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            284..388
                     /locus_tag="ACHXOL_004523"
                     /pseudo
     CDS             284..388
                     /locus_tag="ACHXOL_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          194 a          115 c          119 g          161 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tatcgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctc
//
LOCUS       DAZRUF010000568          580 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZRUF010000568 DAZRUF010000000
VERSION     DAZRUF010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.571"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>580)
                     /gene="ytfI"
                     /locus_tag="ACHXOL_004524"
     CDS             complement(<1..>580)
                     /gene="ytfI"
                     /locus_tag="ACHXOL_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR6811296.1"
                     /translation="NNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWISNYDSLL
                     TLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLRGSGFEYE
                     EIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQAIGDVSL
                     GYALLEDIVSKLKRGKFELQIPGGGIKECDGWY"
BASE COUNT          171 a          118 c           84 g          207 t
ORIGIN      
        1 tataccatcc atcgcattct ttaattccac ctcctggtat ttggagttca aatttacccc
       61 gttttaattt gcttactata tcctccaata aggcataacc taaagacacg tcaccaatcg
      121 cctggtgaat ataaccttta atccttttcg gtatgtcaga ctgttttttc tgcagaatat
      181 cacaggcata caatcttctg ttttgttcta acggaaactc gcggtttttg aaatccatga
      241 atgagttata ctcatttatt gttatgggaa tttcttcgta ttcaaatcca ctacctcgca
      301 atgtcattgt atattcggca ccgttatctt taaggctgaa actgataagg aacttatcca
      361 tggcgttaac agttccattt tttgatgaaa gtacctgata actattttta tcaatctgag
      421 ttgcgcaacg aaagaactcc ctcaaagtta acaggctatc ataattcgaa atccaatctt
      481 ttttcttaaa tgcatgctgg atattgttaa aaaaattgtt tgaatgtgtt tttgtatacg
      541 atgactttac aaataactcc accgcaccaa taaaattatt
//
LOCUS       DAZRUF010000569          577 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZRUF010000569 DAZRUF010000000
VERSION     DAZRUF010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.572"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..294
                     /locus_tag="ACHXOL_004525"
     CDS             <1..294
                     /locus_tag="ACHXOL_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR6811297.1"
                     /translation="NNKLNLSFLSFDDLICARYISQKPISLDPVKDIQAELLQLEAGI
                     KSKTQVIAELGGDPVKVLAEVQAEKDKENKNKEVIQDGNEKPEEGTNETPTGD"
     gene            248..>577
                     /locus_tag="ACHXOL_004526"
     CDS             248..>577
                     /locus_tag="ACHXOL_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFR6811298.1"
                     /translation="MKNQKRELTKPLQAINTESRTIEVAFCSETPVEREINGQIYNEI
                     LLCGSSNADLTRLNNNGAVLFNHNRDDLIGAVVDARVDADRVGRATLRISSTANDEWE
                     MIQEGVLT"
BASE COUNT          199 a          103 c          130 g          145 t
ORIGIN      
        1 aacaataagt taaatctaag tttcttgagc tttgatgatc ttatttgtgc tcggtatatt
       61 tctcaaaagc caattagttt agatcctgta aaggatatac aggctgaact actccagtta
      121 gaagcaggta ttaaatctaa aacacaggtt attgctgagt taggcggtga tccagtgaag
      181 gtattagcag aggtacaggc agaaaaggat aaagaaaata aaaacaagga agtaatccag
      241 gatggaaatg aaaaaccaga agagggaact aacgaaaccc ctacaggcga ttaatacaga
      301 atcgcgtacc atcgaagtag cgttttgttc agaaacccct gtagaacgtg aaatcaacgg
      361 tcaaatctat aacgaaattc ttctatgtgg ttccagtaac gcagacctaa cccgccttaa
      421 taacaatggg gcagtactct ttaaccataa ccgtgatgat cttattggtg ctgtagttga
      481 tgcacgagtg gatgctgatc gcgttggtag agctacatta cgaattagca gtaccgccaa
      541 cgatgaatgg gaaatgattc aggaaggcgt actaacc
//
LOCUS       DAZRUF010000570          575 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZRUF010000570 DAZRUF010000000
VERSION     DAZRUF010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.573"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            90..>575
                     /locus_tag="ACHXOL_004527"
     CDS             90..>575
                     /locus_tag="ACHXOL_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR6811299.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DF"
BASE COUNT          129 a          155 c          153 g          138 t
ORIGIN      
        1 tgcaaacaag ccaatacgcg gctgaaaagg atatgcagga tgcagcgccc cgtctgactt
       61 tcactttacg agacgaagag aggttgatga tgaaaattgg cgtattcgta cctattggca
      121 acaacggctg gctcatttcg acccacgcgc cgcagtacat gccgaccttt gaactgaata
      181 aagccattgt gcagaaagcg gagcattacc atttcgattt cgccctgtcg atgatcaaac
      241 tgcgtggctt tggcggcaaa actgagttct gggatcacaa ccttgagtcg ttcaccttga
      301 tggcgggtct ggcagccgtg acctcgcgca ttcagattta cgccactgct gccaccttaa
      361 cgttacctcc ggcaatcgtc gcccgtatgg ccgcaaccat cgactccatc tctggcgggc
      421 gttttggcgt caacctcgtg actggctggc aaaagcccgt gtatgagcag atgggtatct
      481 ggcctggcga tgactatttc tcccgtcgtt acgactatct caccgaatat gttcaggtgc
      541 tgcgcgacct gtggggcacg gggaaaagcg atttt
//
LOCUS       DAZRUF010000571          564 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZRUF010000571 DAZRUF010000000
VERSION     DAZRUF010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.574"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>564)
                     /locus_tag="ACHXOL_004528"
     CDS             complement(<1..>564)
                     /locus_tag="ACHXOL_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFR6811300.1"
                     /translation="KVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGF
                     IGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSA
                     DLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDL
                     WQDEHLLRLCLRQALQLADVVKLSEEEW"
BASE COUNT          146 a          169 c          128 g          121 t
ORIGIN      
        1 ccattcttct tccgagagct tgacgacatc cgccagttgt agcgcctgcc gcaaacacaa
       61 gcggagcaaa tgctcgtctt gccatagatc ttcacgaata ttaggatcga agctgacaaa
      121 acctccggca tgccggatcg ccgtcatcgc agtaaatgcg ctggtacgcg aaggctcggc
      181 agacaacgca attgaacaga gatgtaacca ttcgccatgt cgccagcagg gcaagtctgt
      241 cgtctctaaa aaaagatcgg cactggggcg gaccataaac gtaaatgaac gttccccttg
      301 atcgttcaga tcgacaagca ccgtggatgt ccggtgccat tcatcttgct tcagatacgt
      361 gatatcgact ccctcagtta gcagcgttct ttgcattaac gccccaaaag gatcatcacc
      421 gacccgacct ataaatccac ttattccgcc taatctggcg attcccaccg caacgttagc
      481 tggcgcgccg ccaggacaag gcagtaggcg cccgtctgat tctggcaaga gatctacgac
      541 cgcatcccct aaaacccata cttt
//
LOCUS       DAZRUF010000572          559 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZRUF010000572 DAZRUF010000000
VERSION     DAZRUF010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.575"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..428
                     /locus_tag="ACHXOL_004529"
     CDS             <1..428
                     /locus_tag="ACHXOL_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR6811301.1"
                     /translation="QLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFKSGRE
                     FAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKK
                     RRPASVAIVAMANKLARTVWAITAHDRKYDRNHVSIRPY"
BASE COUNT          163 a          124 c          156 g          116 t
ORIGIN      
        1 aacagcttac cagcgtggcg aggcagaatg aaacctgtaa gcggttgctg gatattcctg
       61 gcgttggacc acttattgcg acggcagcgg tggccaccat gggggaagca tcagcgttta
      121 aatcggggcg agagttcgcc gcatatgttg gtctggttcc aaaacaaact ggctccggag
      181 ggaaagtacg tctgctgggg ataagcaaac gtggtgacac ttatctcagg acattattta
      241 tccacggtgc aagagcggtg gcattagtag ctaaagagcc tggcccgtgg ataaccgaac
      301 tgaaaaaacg tcgtccagcc agtgtggcaa tcgtcgccat ggcaaacaag ctggcacgaa
      361 cagtatgggc gataaccgcc catgaccgta agtatgacag gaaccacgtc agtatcagac
      421 catattaatc gctgatacca ttaaacaatg aactcttaac aaaagggtga atgctgaaag
      481 gttgctatgg cggccagagt gatgacaaag acaggtaaga ccgtgactca ctaaacctga
      541 acagtatttt gggcttgaa
//
LOCUS       DAZRUF010000573          556 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZRUF010000573 DAZRUF010000000
VERSION     DAZRUF010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.576"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..259)
                     /locus_tag="ACHXOL_004530"
     CDS             complement(<1..259)
                     /locus_tag="ACHXOL_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR6811302.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRK"
     gene            complement(271..>556)
                     /locus_tag="ACHXOL_004531"
     CDS             complement(271..>556)
                     /locus_tag="ACHXOL_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811303.1"
                     /translation="CSTELNSKSQSPALGLGHEFAHAQYCLLDKENFMALLSRTDKKY
                     ENKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
BASE COUNT          171 a          140 c           71 g          174 t
ORIGIN      
        1 cttttctgaa atacttcatt tgcaataaat caattatctt accagcataa agatctctaa
       61 tttcattcac caccacttta tatctgtata tttcattaag accttccttg ctaagcggaa
      121 ctaacgtggt aacatacgat ggaagattaa ttatctctat cgttttcacg ctatctattc
      181 cacacaattc attcactcgc cccatcccca ataccgaacc agaaaataga gacaataaaa
      241 ccatcaaata tttcatcata aaaaacctca ctattctggc gtacccgtta ttttcatcgt
      301 ctgcaacggt ccatccaccc gataaaaagg cagtccacta tgagctcctc tggtacactc
      361 acctaaagtt ttcgccgcac gcgattcaat tattgtaata acccttgcct cttctttatt
      421 ctcatatttc ttatctgttc ggcttaaaag agccatgaaa ttttccttat caagaaggca
      481 atactgcgca tgagcaaact catgtcccaa acctaatgca ggcgattgtg atttcgagtt
      541 taattcagtt gaacaa
//
LOCUS       DAZRUF010000574          555 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZRUF010000574 DAZRUF010000000
VERSION     DAZRUF010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.577"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>555
                     /gene="feaR"
                     /locus_tag="ACHXOL_004532"
     CDS             <1..>555
                     /gene="feaR"
                     /locus_tag="ACHXOL_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR6811304.1"
                     /translation="QEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDASR
                     PCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNNP
                     ALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWIA
                     GETGMSVRSLYRMFADKGLVVAQY"
BASE COUNT          142 a          125 c          146 g          142 t
ORIGIN      
        1 ggcaggaagt aaaaggcagc gacgatgcct ggttttacac cgtttttcag cttagtggtc
       61 aggcaataat ggagcaggat gagcgtcagg tgcagattgg cgctggcgat attacgttac
      121 tcgatgcctc acgcccctgt tcgctttact ggcaggagtc ttctaaacag atttcattac
      181 ttttgccacg cactctgctg gaacaatatt ttccccatca aaaacctgtc tgcgcagaaa
      241 gactggacgc tgacttaccc atggtgcaac tcagtcatcg cctgttacag gagagcatga
      301 ataatccggc actttctgaa acagaaagtg aagctgcgct acaggcgatg gtgtgtctgc
      361 tgcgcccggt acttcatcag cgggaatctg ttcaacctcg ccgtgaacgt cagtttcaaa
      421 aagtggttac gttgatagac gataatattc gcgaagagat attacgcccg gagtggatag
      481 ccggagagac aggtatgtca gtacgtagtt tgtaccgaat gtttgccgat aaaggtttgg
      541 tagtcgcgca atata
//
LOCUS       DAZRUF010000575          554 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZRUF010000575 DAZRUF010000000
VERSION     DAZRUF010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.578"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>554
                     /locus_tag="ACHXOL_004533"
     rRNA            <1..>554
                     /locus_tag="ACHXOL_004533"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          155 a          120 c          171 g          108 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca accc
//
LOCUS       DAZRUF010000576          553 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZRUF010000576 DAZRUF010000000
VERSION     DAZRUF010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.579"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>553
                     /locus_tag="ACHXOL_004534"
     CDS             <1..>553
                     /locus_tag="ACHXOL_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR6811305.1"
                     /translation="LAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRL
                     ALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAA
                     HLAAYAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMS
                     QGKRHNQALIALARRRCDVLFAMM"
BASE COUNT          105 a          186 c          152 g          110 t
ORIGIN      
        1 ctggcagccc agctctgcaa acttgcgcct cgtctgggta aacgccttgc agcagacata
       61 gctcaggcac tggccgaaca aaccgtcgtc gttcccggca cgaatgccgc tgccgtagta
      121 ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc aaagagacga ggtggcgctt
      181 gaggtagaac agcgagttct tgctcaccct ctttacccgg tcctgaccag tatgcccgga
      241 gtcggtgtca ggaccgcagc cagactcctc accgaggtcg cctgccgcgc cttcgcctct
      301 gccgcacatc tcgctgctta tgctggcctt gcgccggtaa ctcggcgatc cggctcgtca
      361 atacgcggtg agcatccctc gcgacggggt aataaagctc tcaaacgggc gttgttcctg
      421 tcggccttcg ccgcgctcag ggatccgctc tccagggctt actacacccg caaaatgagt
      481 cagggaaaac gacacaatca ggcgcttatc gccctggcga gacgacgctg cgacgttctg
      541 ttcgccatga tgc
//
LOCUS       DAZRUF010000577          552 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZRUF010000577 DAZRUF010000000
VERSION     DAZRUF010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.580"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            38..>552
                     /locus_tag="ACHXOL_004535"
     CDS             38..>552
                     /locus_tag="ACHXOL_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR6811306.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAV"
BASE COUNT          159 a          112 c          134 g          147 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gt
//
LOCUS       DAZRUF010000578          547 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZRUF010000578 DAZRUF010000000
VERSION     DAZRUF010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.581"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(1..547)
                     /locus_tag="ACHXOL_004536"
                     /pseudo
     CDS             complement(1..547)
                     /locus_tag="ACHXOL_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          154 a          123 c           99 g          171 t
ORIGIN      
        1 acctccctgt ttttgtatct tctctgcaat atctttctgg caacccatcg catcagttgt
       61 gatgattttt cctttaatat ccagcatgtt aagaagttca ggaatggctg taatctcatt
      121 ggatttctca tccgtcttaa tctgcccgag aatcagactg tgcattgttg agaacgcact
      181 aatgacatga attgctcccc tgcggcactc ttgtcataag agtgccggag cgtttttcca
      241 tcaattgcaa tgacgtcttt atcatctgaa gaatggcagt cacgcatcca gttaataaag
      301 cactcgtgaa attttgcagg actgatacag gatacaactc tggcgatggt atcgtgaaca
      361 ggaataccat tttcaaaatc accatattgt tttaaaaaat cgagatgtgt ttccccaaaa
      421 tcctctatat cttcccagcc ttctgcacca gaaataacgg cacaaatagt caacagtaga
      481 atatccgata atttatgttc cactttccag gcttgtctgt aatcggggat aatagaaata
      541 tgttcca
//
LOCUS       DAZRUF010000579          546 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZRUF010000579 DAZRUF010000000
VERSION     DAZRUF010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.582"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHXOL_004537"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHXOL_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          159 a          128 c          144 g          115 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgcca
//
LOCUS       DAZRUF010000580          545 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZRUF010000580 DAZRUF010000000
VERSION     DAZRUF010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.583"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..545
                     /locus_tag="ACHXOL_004538"
                     /pseudo
     CDS             1..545
                     /locus_tag="ACHXOL_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT          163 a          114 c          111 g          157 t
ORIGIN      
        1 taaattatgt ctggcaaccg gatgtctttg gattccccgc aacgcttcca caaattctca
       61 aaaaatcagg cattaactat attgcatcgc aaaagctctc acaaaacaag attaataaat
      121 ttaataatta ccttttccgc tggcagggcc tggatggctc agaaattctg atgcataact
      181 ttcctgaaga cacttatgat agccgagcac gtgctcgctc tttagaatat attgaacaaa
      241 actataatga aaaagagatc tgtccctatg cgcttatggt gtatggtgta tggtgtcggt
      301 gatggcggcg ctggccccgg tgaggaacat atagaacgat taacacgtat acgcaatatt
      361 gatggcttac ctcatgttga tttcagccgt gttgataagt tcttcactta cgccgacgcc
      421 tttcgcgaaa gcttgcccat tatttcaggt gaactttatt tcgaggctca tcaagggtgc
      481 tttacatcag agtcagcaac taaagctcat aatcgaaata tggaaaataa attacatgat
      541 gctga
//
LOCUS       DAZRUF010000581          541 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZRUF010000581 DAZRUF010000000
VERSION     DAZRUF010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.584"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHXOL_004539"
     CDS             complement(<1..140)
                     /locus_tag="ACHXOL_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR6811307.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHXOL_004540"
     CDS             complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHXOL_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR6811308.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT          183 a          120 c           98 g          140 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 t
//
LOCUS       DAZRUF010000582          539 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZRUF010000582 DAZRUF010000000
VERSION     DAZRUF010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.585"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            82..228
                     /locus_tag="ACHXOL_004541"
                     /pseudo
     CDS             82..228
                     /locus_tag="ACHXOL_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(253..>539)
                     /locus_tag="ACHXOL_004542"
     CDS             complement(253..>539)
                     /locus_tag="ACHXOL_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811309.1"
                     /translation="ITLNDINLALSFILVINNTYVNINIGAKRIRDTGCKAIYFVLIS
                     LGLTLFSIISAAIEGVRCESLSVQLNVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          162 a          105 c          104 g          168 t
ORIGIN      
        1 ctctgaagag aacctcgtta atgattggtc ttgctcattg ttaataacaa aatgttgaat
       61 ttaccgatta ggaaactttt tcctacgcgg attgcctcca gctatggcat gaactttgag
      121 tttatgccag aactgaagtg gagctttggc taccctggcg cgattatctt tatgatcctt
      181 gcgggcctga caccgtatct gtactttaag cgtaagaact ggctgtaact atacgatgtc
      241 gctgtgagta agttagtgtg ctgacagcga cattttagcc gagggaatcg cagataacat
      301 aattgttgtg aataatatcc atgccacgtt gagttggacg cttaatgact cacatctcac
      361 cccttcaatt gctgcagaga taatagaaaa aagtgtcaaa cccaatgaaa tcaatacaaa
      421 gtagattgcc ttacatccag tatctcttat tctttttgct ccaatattga tatttacata
      481 ggtattatta ataaccagaa taaaactcaa agccaggttg atatcattca aagttatta
//
LOCUS       DAZRUF010000583          532 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZRUF010000583 DAZRUF010000000
VERSION     DAZRUF010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.586"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..532
                     /gene="yfdF"
                     /locus_tag="ACHXOL_004543"
                     /pseudo
     CDS             1..532
                     /gene="yfdF"
                     /locus_tag="ACHXOL_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530666.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YfdF"
BASE COUNT          194 a           89 c          109 g          140 t
ORIGIN      
        1 aaatgatctt cagcgcatta aaagaaatat atgatggaga tcctggtttt atcttcgata
       61 agatatcaca caaactcaga catacggtaa cggaatttga tgagagcggg aatagcgaac
      121 caacggactt atttacctgg tacggtaaag ataaaaaagg caactctctc gctattgtaa
      181 ttaaaaataa aaacggaaat gattacttat ctctcggtta ctacgatcag gacgactacc
      241 acattcaaag aggaattcgt attaatggtg atagtctcac ccaatattgt agtgaaaaag
      301 ccaggaatgc ttcagcaatg cttcagcgtg gtttgaaagc agtaaagcta tcatggcaga
      361 atcatttgca acaggttccg atcatcaggt tgtaaacgag ctcaacgggg aaagactgag
      421 agaacctaac gaagttttta aacgtttagg tcgagcaata agatataatt ttcaagtgga
      481 cgatgcaaaa tttagacgcg acaatgtaaa agaaataatt tctaatttat tc
//
LOCUS       DAZRUF010000584          529 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZRUF010000584 DAZRUF010000000
VERSION     DAZRUF010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.587"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>529
                     /locus_tag="ACHXOL_004544"
     CDS             <1..>529
                     /locus_tag="ACHXOL_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR6811310.1"
                     /translation="GGFVLHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTID
                     RWGYGVTSALSPYHENRIALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSA
                     IMNITRSDGKNIPFAADIYDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVS
                     CLAHYQQSSEAEKIAQ"
BASE COUNT          154 a          102 c          134 g          139 t
ORIGIN      
        1 cggtggtttt gtattgcata gcggcggcct gaccttcagt aatgacagtt ttagcgactc
       61 cgatacactg gcggtagttc aggctccagg tgctcaagga gcgcgaataa attatggcaa
      121 cagtactatc gatcgatggg gttatggtgt caccagcgct ctttctcctt atcatgaaaa
      181 ccgtatcgcg ctggatatca acgatcttga gaacgatgtt gaattaaaaa gtaccagtgc
      241 agtagctgta ccgcgtcagg gttcagtcgt ctttgctgat tttgaaaccg tgcaagggca
      301 atcagccatt atgaacatca cacgaagtga tggtaaaaat attccatttg ctgcagatat
      361 ttatgatgag caaggcaatg tcattggtaa tgttggacag ggtggacaag catttgttcg
      421 tggcattgag cagcagggaa atatcagcat taaatggctc gaagaaagta aacccgtaag
      481 ttgtcttgcg cattatcaac aaagttcaga agcagaaaaa atagcgcaa
//
LOCUS       DAZRUF010000585          525 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZRUF010000585 DAZRUF010000000
VERSION     DAZRUF010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.588"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>525)
                     /locus_tag="ACHXOL_004545"
     CDS             complement(<1..>525)
                     /locus_tag="ACHXOL_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR6811311.1"
                     /translation="GETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWM
                     RDCHSSDDKDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEI
                     TAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKF
                     PLKELNNPEHDSYA"
BASE COUNT          140 a          119 c           96 g          170 t
ORIGIN      
        1 attgcgtaac tgtcatgctc tggattattt aattctttca gcggaaattt ttcctcaaag
       61 gctttattaa gccgcccctg gtttcctttt accgcgaata aataatcacc gccctgtttt
      121 tgtatcttct ctgcaatatc tttctggcat cccatcgcat cagttgtgat gatttttcct
      181 ttaatatcca gcatgttaag aagttcagga atggctgtaa tctcattgga tttctcatcc
      241 gtcttaatct gcccgagaac cagactgtgc attgttgaga acgcactaat gacatgaatc
      301 gctcccttgc ggcgactctt gtcataagag tgccggagcg tttttccatc aattgcaatg
      361 acgtctttat catctgaaga atggcagtca cgcatccagt taataaagca ctcgtgaaat
      421 tttgcaggac tgatacagga tacaactctg gcaatagtat cgtgaacagg aataccattt
      481 tcaaaatcac catattgctt caaaaaatcg agatgtgttt cacca
//
LOCUS       DAZRUF010000586          522 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZRUF010000586 DAZRUF010000000
VERSION     DAZRUF010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.589"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>522)
                     /locus_tag="ACHXOL_004546"
     CDS             complement(<1..>522)
                     /locus_tag="ACHXOL_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811312.1"
                     /translation="FLGVTTWIFMAAGGAESVAVYVNDVKGGSKSFVKVIILAGIFIG
                     VLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNRFVGLVSFTAMFGS
                     LLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIVIPLMIIPMLGSNT
                     VQDLMNTIINMTA"
BASE COUNT          143 a          141 c          133 g          105 t
ORIGIN      
        1 cggcggtcat attaataata gtattcatca gatcctgcac ggtattggaa ccgagcatcg
       61 ggataatcat cagcgggatg acgatcagga actggatcca cgctgcgcgc gccggaacgc
      121 cgttttcgtt cagttcgacg gttttcttac caaagatgcc ttccgggatt tcggagaaga
      181 aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacatcgcg gtaaaggaca
      241 ccagaccgac aaagcgattc atcaacgctt ccggtagacc aaaatacgcc gccatgccgt
      301 ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg aagacgttaa
      361 tcagcaccga ggagacggaa tacagtacgc cgataaaaat tccggcgagg atgatcactt
      421 taacgaacga tttcgaaccg cctttgacgt cgttaacgta cacagcgacg gactccgcgc
      481 cacctgcggc cataaagatc caggtggtaa cgccgaggaa cg
//
LOCUS       DAZRUF010000587          510 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZRUF010000587 DAZRUF010000000
VERSION     DAZRUF010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.590"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..183
                     /locus_tag="ACHXOL_004547"
     CDS             <1..183
                     /locus_tag="ACHXOL_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR6811313.1"
                     /translation="LKEELTRRISKRYDDVEVIVKATSNDGLSVTRTADKDSAKTFVQ
                     ETLKDTWESADEWFVH"
BASE COUNT          164 a          125 c          110 g          111 t
ORIGIN      
        1 ttaaaggaag aattaacccg acgcatcagt aaacgttatg acgatgtaga ggtgatcgta
       61 aaagccacca gcaacgatgg cctttctgtt acgcgcaccg ccgataaaga ttcagctaaa
      121 acttttgttc aggaaactct gaaagatacc tgggagtctg ctgacgagtg gtttgttcac
      181 taattagcac gtaaaatcag taacggctgg aaatcattca atactcgcac tatcggaagt
      241 tcaccagcca gccgcagcac gttcttgcat acgacgtgtc tgcggtctca actccgcccg
      301 cccccgccaa tcatgattgg gcagtaccag tacagcacta gcaaagacag gaaattagca
      361 gtctcagcaa aacaccgacc agacggtgag aagacaaaaa gatacgcaaa gatgccgcgg
      421 ttccagaaga ataaaaaacc gctaatggac agcctcctta acacagtttt ttatatacat
      481 gattcactga taccagtggc attaatagct
//
LOCUS       DAZRUF010000588          503 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZRUF010000588 DAZRUF010000000
VERSION     DAZRUF010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.591"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            53..>503
                     /locus_tag="ACHXOL_004548"
     CDS             53..>503
                     /locus_tag="ACHXOL_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR6811314.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLK"
BASE COUNT          120 a          122 c          131 g          130 t
ORIGIN      
        1 ccgcatgggt gagctgttgc tacaccccgg tatttccacc acggaggcca gcatgaacaa
       61 caataacaca ttgtacgtcg ggttagatgt tcacaaagaa tcgattactg tcgcttatgc
      121 tatcaattca gaacctgttg aactgatggg taaaattggc acatcaccta ctgatattca
      181 gaatctttgt aaacgtctca ggtcaaagtc atcgcaggtc agtatcgttt atgaagcggg
      241 gccctgtggt tatggacttt atcgtcggct ggtgaaatcc ggttttgact gcatggtctg
      301 cgccccctca cttatcccga aaaaaccggg ggagcgggtt aaaaccgatc gccgtgatgc
      361 catcagactt gtgcgttcac tgcgtgcagg agatctttct gctgtctacg tacccggcat
      421 tgaagatgag gcattccggg atttggcccg ggcatgggca tctgcccgcg atgatttacg
      481 gcatgcaagg caacgtctga aat
//
LOCUS       DAZRUF010000589          502 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZRUF010000589 DAZRUF010000000
VERSION     DAZRUF010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.592"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>502
                     /locus_tag="ACHXOL_004549"
     CDS             <1..>502
                     /locus_tag="ACHXOL_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR6811315.1"
                     /translation="DIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGR
                     CVAACGDFGLVMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSV
                     YDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQ
                     LLILKGS"
BASE COUNT          166 a           91 c          100 g          145 t
ORIGIN      
        1 atgatattta tcagaatgtt gattacaaac cccatcaaga tgattattct tcagctgaaa
       61 aatttctcac tcacttcaac aaaaaatgca aaaaccagac tttggcgttg atttccagcc
      121 gtcctgaggg gcgttgcgtt gctgcctgcg gtgatttcgg gctagttatg aaagcatatt
      181 ttgacaagat ggaatcaaat ggcatcagtg ttatggcagc catattactg gtggataacc
      241 atgctttgac ggtccggcta agaataaaga acacaactga aggatgtacc cattacgtgg
      301 tttcggttta tgatcctaat gtaactaacg acaaaataag aatcatgagc gaaagcaaag
      361 aggatattaa acactattct ctgatggatt ttatgaatgt agattatagc ctcctgaaat
      421 ggtcaaatga tcatgttatt aaccaatctg ttgcaataat tccagcactt ccgaaagaac
      481 agctattgat attaaaagga tc
//
LOCUS       DAZRUF010000590          497 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZRUF010000590 DAZRUF010000000
VERSION     DAZRUF010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.593"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..497
                     /locus_tag="ACHXOL_004550"
                     /pseudo
     CDS             1..497
                     /locus_tag="ACHXOL_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          175 a          111 c           79 g          132 t
ORIGIN      
        1 gcaaccaact cccaccttaa accaactggt ccggataatt cagatgttct cctgttcaaa
       61 aagctattct ccccttcgaa attaatgaca attatcggtg atgaaattcc tcttatatct
      121 gaaaaacagt cgctttcaaa ggtgctttta aatgatgaga ataatgaact gagtgatggt
      181 acaaacttct gggataaaaa tcgtcaatta accacagatg aaatagcttg ctatcttcag
      241 aagatcgccg ctaatgcaaa aaatactcaa gtcaattatc ctactggtct ctacgtcccc
      301 tactccacca gaactcacct ggaagacgct ctcaatgaaa atattaagag cgatccatca
      361 tggccgaatg aagtccagtt attccccata aatactggcg gacactggat attagtttcg
      421 ctacagaaaa tagtaaataa aaaaataata aactacaaat aaaatgcgtc atattcaact
      481 cattgcgtgc actaggc
//
LOCUS       DAZRUF010000591          496 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZRUF010000591 DAZRUF010000000
VERSION     DAZRUF010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.594"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(10..363)
                     /locus_tag="ACHXOL_004551"
     CDS             complement(10..363)
                     /locus_tag="ACHXOL_004551"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811316.1"
                     /translation="MFSIGNVSINCTINPEDNNLLPHYVWIYGHTSTQLNQYDSDIIF
                     IINDKKYPAPSVDGTRMNKNAWVYFIDAISEATQFDVLVNGKKVDSYTANIKNVKKTL
                     GNKFYDSCWNTWFQE"
BASE COUNT          141 a           95 c           95 g          165 t
ORIGIN      
        1 cgttatcaat tattcctgga accaggtgtt ccagcagctg tcgtagaatt tgttacccaa
       61 agtctttttc acatttttga tatttgcggt atagctgtcg actttcttac cgtttactaa
      121 aacgtcgaat tgcgtggctt cactgatagc atcgataaag tatacccatg cattcttatt
      181 cattcgtgtt ccgtcgacag atggggccgg atacttttta tcgttgatga taaaaataat
      241 atcagaatcg tactgattta gctgggtgga ggtatgaccg taaatccaca catagtgtgg
      301 tagcaaattg ttgtcttctg gattaatcgt gcagttaata gaaacattac cgatactgaa
      361 catatcagtg ccagcaatac tgttacgcgt ctacaatgcc gattctgttt gtgctacggt
      421 tgcacttaca gttaaaacaa cagctaaaag aatgtttaac agtttcatga gttttctccc
      481 caaaattaaa taaatt
//
LOCUS       DAZRUF010000592          496 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZRUF010000592 DAZRUF010000000
VERSION     DAZRUF010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.595"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHXOL_004552"
     tRNA            54..130
                     /locus_tag="ACHXOL_004552"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHXOL_004553"
     tRNA            173..248
                     /locus_tag="ACHXOL_004553"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          139 a          103 c          126 g          128 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcgca agaaacatct
      421 tcgggttgtg aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga
      481 tgaaggacgt gctaat
//
LOCUS       DAZRUF010000593          491 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZRUF010000593 DAZRUF010000000
VERSION     DAZRUF010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.596"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..248
                     /gene="tnpA"
                     /locus_tag="ACHXOL_004554"
                     /pseudo
     CDS             1..248
                     /gene="tnpA"
                     /locus_tag="ACHXOL_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          143 a          120 c          105 g          123 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gtgactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtgttggc tattttgcca
      181 gtagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccac gccttatatc cctgccctga aggacggggt tttacggcgc
      301 accggataag cctggagatt atctacacca gcatggacat cattgtacta tgccccgtac
      361 atgccccatc acatcctcaa catcaaagta ttagagcctt gcaaaccatt atgcgtctca
      421 gtttgtctca tctatcttac gcaaaaaaaa tagaactaat atcaagcggc gctatattgc
      481 gtattaaata a
//
LOCUS       DAZRUF010000594          490 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZRUF010000594 DAZRUF010000000
VERSION     DAZRUF010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.597"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>490)
                     /locus_tag="ACHXOL_004555"
     CDS             complement(<1..>490)
                     /locus_tag="ACHXOL_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR6811317.1"
                     /translation="CHTHINPKAINYPLSFLLQNTGYTIAERAVVTFTAKGDFRLCGF
                     NWEIMEELTESKRIDLPSAPTAPKGKWRSVCGVTERMPYAGGALETPLSSIISKEIGP
                     LLSLTPTERDKNDFYYKEKRNEPVQVVSLECEEWRHQVKEEIFPLFVYTANKPKKVNG
                     AIE"
BASE COUNT          127 a          108 c           89 g          166 t
ORIGIN      
        1 ctcaattgca ccattcactt tctttggttt gttggctgta tatacgaaca acgggaatat
       61 ttcttctttg acttgatgac gccactcttc gcattccagc gatacaactt gaactggctc
      121 atttctcttt tctttataat aaaaatcgtt tttatccctt tctgttggag taagagataa
      181 caaaggacca atttcttttg aaattatact ggatagcggt gtttccaaag ctcccccagc
      241 gtaaggcatt ctttctgtta cgccacatac actacgccat tttcctttgg gggcggtagg
      301 ggctgaaggt aggtcaatcc ttttgctttc agtcagctct tccattatct cccaattaaa
      361 tccacacaga cgaaaatctc cttttgctgt aaatgttacc acagcacgtt cagcgatagt
      421 ataacctgtg ttttgtaata aaaacgacag cgggtaattt attgccttcg gattaatatg
      481 agtatgacag
//
LOCUS       DAZRUF010000595          489 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZRUF010000595 DAZRUF010000000
VERSION     DAZRUF010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.598"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHXOL_004556"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHXOL_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR6811318.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAZRUF010000596          485 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZRUF010000596 DAZRUF010000000
VERSION     DAZRUF010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.599"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>485
                     /locus_tag="ACHXOL_004557"
     CDS             <1..>485
                     /locus_tag="ACHXOL_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR6811319.1"
                     /translation="PLGAISFYAARSSSKLNNDTRHEGTSYQVAYNKYLVQTATRFSV
                     AAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSL
                     SALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNKEEERFNLFISIPFYW
                     G"
BASE COUNT          148 a           97 c           98 g          142 t
ORIGIN      
        1 ccgctgggtg ccatctcttt ttacgctgcc cgatcgagta gtaagttaaa taatgatacc
       61 agacacgaag gtacgagcta tcaggtcgcc tataataaat atctagtaca aacagccact
      121 cgatttagcg tggccgcctg gcgttatgct tcgcaggatt acaggacatt cagcgaccat
      181 ctttacgaaa atgataaaca ctatcatcag agtgattatg atgattttta tgatattggc
      241 agaaaaaata gcctttctgc caatattatg caacctttat ccaataatct gggaaatgta
      301 tcattaagtg cgctttggcg gaattactgg gggcgaagtg gaaatgctaa agattaccaa
      361 ttcagttact ccaataactg gcaacacatt agttatactt tctctgccag ccaatcttat
      421 gatgaaaata ataaagaaga ggagcgtttt aatctgttta tctccattcc tttctactgg
      481 gggga
//
LOCUS       DAZRUF010000597          484 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZRUF010000597 DAZRUF010000000
VERSION     DAZRUF010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.600"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..172)
                     /gene="tomB"
                     /locus_tag="ACHXOL_004558"
     CDS             complement(<1..172)
                     /gene="tomB"
                     /locus_tag="ACHXOL_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR6811320.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIAT"
BASE COUNT          136 a          104 c          106 g          138 t
ORIGIN      
        1 aggtcgcaat atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt
       61 taacccagcc atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac
      121 agagaaactt aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc
      181 cccttcttag cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg
      241 acttgccctc tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt
      301 attacgaaaa attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac
      361 taacccgcag caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc
      421 cagatgaaat tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga
      481 atga
//
LOCUS       DAZRUF010000598          479 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZRUF010000598 DAZRUF010000000
VERSION     DAZRUF010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.601"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            28..420
                     /gene="ydeI"
                     /locus_tag="ACHXOL_004559"
     CDS             28..420
                     /gene="ydeI"
                     /locus_tag="ACHXOL_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR6811321.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          136 a           98 c          121 g          124 t
ORIGIN      
        1 caaaccaaac gcaattaagg agttagtatg aaatttcagt ccattgtatt agcaagtttt
       61 cttgtcatgc cttatgcgtt ggcagatgac cagggcggtt taaaacagga tgcagcgcca
      121 ccgccgcctc atgcaataga agatggctat cgcggaaccg atgatgcaaa aaaaatgacc
      181 gttgatttcg caaaaaacat gcacgatggc gcttcggttt cactacgcgg taatttgatt
      241 tctcacaaag gagaggaccg ttacgttttt cgcgataaga gcggtgaaat taatgttgtt
      301 attcctgcgg ccgtctttga tggacgagaa gttcagccgg accagatgat caacattagc
      361 ggcagtctgg ataagaaaag cgcgccggcc gtcgttcggg tcactcattt gcagaaataa
      421 aaataaatta gcctgatggc ctgaactact gggccattag ggtattgggt tatgctatt
//
LOCUS       DAZRUF010000599          472 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZRUF010000599 DAZRUF010000000
VERSION     DAZRUF010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.602"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            89..448
                     /locus_tag="ACHXOL_004560"
                     /pseudo
     CDS             89..448
                     /locus_tag="ACHXOL_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          114 a          112 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DAZRUF010000600          467 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZRUF010000600 DAZRUF010000000
VERSION     DAZRUF010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.603"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>467
                     /locus_tag="ACHXOL_004561"
     CDS             <1..>467
                     /locus_tag="ACHXOL_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR6811322.1"
                     /translation="VKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTA
                     VRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALG"
BASE COUNT          119 a          108 c          152 g           88 t
ORIGIN      
        1 gtgaaagaaa gtaatcagcg atggtgctct gacgggttcg agttccgctg tgataacgga
       61 gaaaaactgc gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
      121 gtcactacgg gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      181 cgccgcttcg gcaacgagct tccggcgtct ccagtagagt ggctgacgga taatggttca
      241 tgctaccggg ctaatgaaac acggcagttt gcccggatgt tggggcttga accgaagagc
      301 acggcggtgc ggagtccgga gagtaacggc atagcagaga gcttcgtgaa aacgataaag
      361 cgtgactaca tcagtgtcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca
      421 gaggcgttcg agcattataa cgaatggcat ccgcatagtg cactggg
//
LOCUS       DAZRUF010000601          464 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZRUF010000601 DAZRUF010000000
VERSION     DAZRUF010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.604"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(7..363)
                     /locus_tag="ACHXOL_004562"
     CDS             complement(7..363)
                     /locus_tag="ACHXOL_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR6811323.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
BASE COUNT          163 a           92 c           99 g          110 t
ORIGIN      
        1 ctaagattaa tttgaaccaa acttcggatc gtttccgtaa cgattgctgc cagaagtacc
       61 ttctgtgcag aaaaaaacca gaagcacgag ccagccaata aacggaacaa aaaagagcaa
      121 ggcccaggca cctgaacggt cggtatcgtg taagcgacgg atagcaagcg caaggacggg
      181 aaggaaggtt gccagcaaat aaagcataga cagatatgga agctccagac ctaaaatcaa
      241 ttgaatgaca ttgataatag cgccgacgat ggcgttaatc agagtaaaca tccagtactc
      301 tttacgacgt gcgcgaccag agaaaccaac ataatttttt aatacggata aataccactg
      361 cataaaaaca tccctttaat taaaagtgac gaaagtctat tccgggtatt gtgtaaatgc
      421 tattcgtatc tttctttttc ggatgttata aaggttttga tatt
//
LOCUS       DAZRUF010000602          461 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZRUF010000602 DAZRUF010000000
VERSION     DAZRUF010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.605"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            36..440
                     /gene="stpA"
                     /locus_tag="ACHXOL_004563"
     CDS             36..440
                     /gene="stpA"
                     /locus_tag="ACHXOL_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR6811324.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT          132 a          100 c          116 g          113 t
ORIGIN      
        1 cttttttgtt ttggcgttaa aaggttttct ttattatgtc cgtaatgtta caaagtttaa
       61 ataacattcg caccctccgt gcgatggctc gcgaattctc cattgacgtt cttgaagaaa
      121 tgctcgaaaa attcagggtt gtcactaaag aaagacgtga agaagaagaa cagcagcagc
      181 gtgaactggc tgagcgtcag gaaaaaatta gcacctggct ggagctgatg aaggctgacg
      241 gaattaaccc ggaagagtta ttgggtaata gctctgctgc tgcaccacgc gctggtaaaa
      301 aacgccagcc gcgtccggcg aaatataaat tcaccgatgt taacggtgaa actaaaacct
      361 ggaccggtca gggccgtaca ccgaagccaa ttgctcaggc gctggcagaa ggtaaatctc
      421 tcgacgattt cctgatctaa ttactgaggc cggatgtcgc t
//
LOCUS       DAZRUF010000603          458 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZRUF010000603 DAZRUF010000000
VERSION     DAZRUF010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.606"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            2..388
                     /locus_tag="ACHXOL_004564"
     CDS             2..388
                     /locus_tag="ACHXOL_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR6811325.1"
                     /translation="MKGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDV
                     YNSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNII
                     CSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          135 a           96 c          122 g          105 t
ORIGIN      
        1 aattaaaggg caggttgttg aacttgtacg tctgagcggt cgcgagacgt tgcggcaact
       61 ggaagccaag acaggtgcga caagatatct gatgagtgtt ctcgccagag agctggttgc
      121 cagtggcgat gtatacaact ctggttacgg gttattcccg tctgaacagg cgcgtaagga
      181 ctggcaaaac gcccgcaaaa aactctcaag ggcaaatctg aagaaaccat ctgtggttga
      241 tcctgacctt atctggtcat taccagacgg agaaatacgc cgctacgaca ggcgtcaaaa
      301 cataatctgt agcgagtgcc ggaagagcga agctatgcag cgtgtactgg ctttctatca
      361 gggtaatttt cagaaggtgc tgttgtgagc caaattaaca atcggaactt cgtgaagaga
      421 aagcataatc caaaatctga ataattaagt tcagcact
//
LOCUS       DAZRUF010000604          458 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZRUF010000604 DAZRUF010000000
VERSION     DAZRUF010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.607"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>458
                     /locus_tag="ACHXOL_004565"
     CDS             <1..>458
                     /locus_tag="ACHXOL_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR6811326.1"
                     /translation="HNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNN
                     KILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTID
                     GLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEV"
BASE COUNT          171 a           84 c           83 g          120 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtg
//
LOCUS       DAZRUF010000605          457 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZRUF010000605 DAZRUF010000000
VERSION     DAZRUF010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.608"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>457
                     /gene="pagP"
                     /locus_tag="ACHXOL_004566"
     CDS             <1..>457
                     /gene="pagP"
                     /locus_tag="ACHXOL_004566"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR6811327.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRF"
BASE COUNT          117 a          107 c          119 g          114 t
ORIGIN      
        1 acgtttagag aaaatattgc acaaacctgg caacagcctg aacattatga tttatatatt
       61 cctgccatca cctggcatgc acgtttcgct tacgacaaag aaaaaaccga tcgctataac
      121 gagcgaccgt ggggtggcgg ttttggtcag tcgcgatggg atgaaaaagg aaactggcat
      181 ggcctgtatg ccatggcatt taaggactcg tggaacaaat gggaaccgat tgccggatac
      241 ggatgggaaa gtacctggcg accgctggcg gatgaaaatt ttcatttagg tctgggattc
      301 accgctggcg taacggcacg cgataactgg aattacatcc ctctcccggt tctactgcca
      361 ttggcctccg tgggttatgg cccagtgact tttcagatga cctacattcc gggtacctac
      421 aacaatggca atgtgtactt tgcctggatg cgctttc
//
LOCUS       DAZRUF010000606          453 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZRUF010000606 DAZRUF010000000
VERSION     DAZRUF010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.609"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>453
                     /locus_tag="ACHXOL_004567"
     CDS             <1..>453
                     /locus_tag="ACHXOL_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR6811328.1"
                     /translation="AVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQ
                     RAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFA
                     DFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
BASE COUNT          132 a           86 c          103 g          132 t
ORIGIN      
        1 cgcggtaaaa cgtgccgata gccgctttga acttattcag aaaaacgttg gctatttctt
       61 taaagatatt gaacgttcgg ccctgacatt aaaggactca ctgtatttat taaaaaatac
      121 agaggagatt caacgcgccg tgattctgaa aatggaaatg atgccatttt tagactcggt
      181 gggactggta cttgatgata ataaatatta tctcttttcg cggagggcga atgataaaat
      241 cgttgtttat catcaggaac aagtaaatgg accgcttgtc gacgagtcag ggcgggttat
      301 ttttgccgat tttaacccat cgaaacgacc gtggtcggtg gcttcagatg actctaacaa
      361 cagctggaat ccggcataca attgctttga tcgtccgggt aaaaaatgta tctcttttac
      421 gctacgtatc aacggcaaag atcacgattt gtt
//
LOCUS       DAZRUF010000607          446 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZRUF010000607 DAZRUF010000000
VERSION     DAZRUF010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.610"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..256
                     /locus_tag="ACHXOL_004568"
     CDS             <1..256
                     /locus_tag="ACHXOL_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811329.1"
                     /translation="VIIEIKNTVSGIKGLNLRTSIAVDGSAELDGDEGTLAGMVALLV
                     LNKSQKIINESAHEAIEILKNDSVITGGRVTEMAVEKTCH"
BASE COUNT          140 a           74 c          121 g          111 t
ORIGIN      
        1 agtaattatt gaaattaaaa atacagtatc tggaattaaa ggactgaatt tgcgcaccag
       61 cattgcggtg gacggtagtg cggaactgga cggtgatgag ggtacacttg ctggcatggt
      121 ggcgttactg gtgcttaata aaagccagaa aataattaat gagtcagccc atgaagctat
      181 cgagattttg aaaaacgata gtgtcatcac cggtggccgt gtcactgaaa tggctgttga
      241 aaaaacatgc cactgaaagg aacgctgaca atgaacgtca aaatccgaaa tgaaatccgg
      301 gcattaattc ggattaaaga gcgtaataac aatggtggag acttgcgcga atttatttgt
      361 gcgcgcgaag ttgaggggta tggcgaaaag acttacctga ttgcatttga ccattacagc
      421 atttgtgcgc gttattgcgg agaggc
//
LOCUS       DAZRUF010000608          440 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZRUF010000608 DAZRUF010000000
VERSION     DAZRUF010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.611"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>440)
                     /locus_tag="ACHXOL_004569"
     CDS             complement(<1..>440)
                     /locus_tag="ACHXOL_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endochitinase"
                     /protein_id="HFR6811330.1"
                     /translation="ALPALAMEAWNNQQGGNKYQVIFDGKIYENAWWGFSTNCPGKAK
                     ANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGSATPAQG
                     TVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDA"
BASE COUNT          114 a          109 c           95 g          122 t
ORIGIN      
        1 ttcgcatcac ccggacagtt agaagaggct acccaccaag catttttata taccgcacca
       61 ttaaagacga cataccaggt ctgaccgccc tgctgtttat tccaggcaac tacagaagaa
      121 ttagacggaa cagtgccctg tgctggtgta gccgaaccgc cattagccgg cgtattggat
      181 gcaggcgtat ttgaatttga agaagatgag ctgccgctct tttcgcagga aagtgtattg
      241 ccaaactgac taatttcagc agctgttgcg gtacgcttta aacgccacgg gttagttgca
      301 tcatttgctt tcgcttttcc cgggcaattt gtagaaaaac cccaccaggc attttcataa
      361 attttgccat cgaaaataac ctgatattta ttaccacctt gttggttatt ccatgcttcc
      421 attgccaatg ctggcagagc
//
LOCUS       DAZRUF010000609          437 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZRUF010000609 DAZRUF010000000
VERSION     DAZRUF010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.612"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>437
                     /locus_tag="ACHXOL_004570"
     CDS             <1..>437
                     /locus_tag="ACHXOL_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR6811331.1"
                     /translation="HIAANIQFPPNFDESKQYPAIISVHPFGSCKEQTSGNIYGKALA
                     EKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLPYVNAERIGVLGVC
                     GGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGA"
BASE COUNT          109 a           85 c          123 g          120 t
ORIGIN      
        1 ggcatattgc tgccaatatt cagtttccac caaattttga tgagtcaaaa cagtatccgg
       61 cgattatcag cgtccatcct tttggcagtt gtaaggagca gacgtctggc aatatctatg
      121 gcaaggcatt ggctgaaaaa ggctatctcg ttctcgctta tgacgccagt ttccagggag
      181 aatctggtgg ggagcctcgc tggatagaag atccgacaca gcgcgtagag gatattagcc
      241 gtgttatcga ttatgcagta actctgccat atgttaatgc tgaacgtatt ggcgtgctgg
      301 gagtgtgtgg gggtggtggc tatgccatta atgccgcgct gacagaaaaa cgaattaaag
      361 cagtagtgag tattacgggt gttaatatcg ggcgtctttt tcgggaaggt ttcagcaatt
      421 acgatccgat tggcgca
//
LOCUS       DAZRUF010000610          433 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZRUF010000610 DAZRUF010000000
VERSION     DAZRUF010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.613"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..112)
                     /locus_tag="ACHXOL_004571"
     CDS             complement(<1..112)
                     /locus_tag="ACHXOL_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811332.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKF"
     gene            complement(368..>433)
                     /locus_tag="ACHXOL_004572"
     CDS             complement(368..>433)
                     /locus_tag="ACHXOL_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR6811333.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          126 a           89 c           86 g          132 t
ORIGIN      
        1 caaactttct ttttaccgtc caataatcaa tatttctatt gtgattgtta accactgcgt
       61 gcattggcgg gattgaaggg tgaaaaatat gtgcaattgg gaagcctttc attgatgcct
      121 gccttgtcgt catgacaaac aataatagat gcttattttc ctcgcagggt tctttcgcac
      181 tatcaaaaaa cgctcattta cccggcgtta cagtaaacga agcaacattc tgtggcctac
      241 gacgatcaaa agctctgtga aaatttccct tgttgtcttt gtctgacctt atcgcggcga
      301 tcagcaatta ttcgttttgt gatcctcaac acaaaatgct attcgtcgcc tatgagggga
      361 gaagaattta tggaaaacga ttgcattcac aaaatgacgt cctgtcagaa gagaaaaagt
      421 tgacgttaat caa
//
LOCUS       DAZRUF010000611          432 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZRUF010000611 DAZRUF010000000
VERSION     DAZRUF010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.614"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>432
                     /locus_tag="ACHXOL_004573"
     CDS             <1..>432
                     /locus_tag="ACHXOL_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR6811334.1"
                     /translation="YWASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYL
                     SFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYT
                     MNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLST"
BASE COUNT          124 a           92 c          103 g          113 t
ORIGIN      
        1 ttactgggct tccggacaaa atcgtagcaa ttactctatt ggctacagta acagtgcatc
       61 ctggggcagc tacagtgtca gtgcccagcg ttcatggaat gaagacggcg acactgacga
      121 tagcgtttat ctcagtttca ccattcctat tgaaaaatta cttggcactg aacaacggac
      181 ttcaggtttc cagagtattg atactcaaat gagcagtgac tttaagggta ataaccaact
      241 caacgtcagt agcagtggct atagtgataa cgctcgcgtc agttatagcg taaatactgg
      301 ctatacgatg aataaagcca gcaaagattt gagttatgtt gggggttatg ccagctatga
      361 atcaccgtgg ggaaccctgg caggttcagt ttctgcaaat agcgataaca gtcgtcaagt
      421 gtctctcagc ac
//
LOCUS       DAZRUF010000612          430 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZRUF010000612 DAZRUF010000000
VERSION     DAZRUF010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.615"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>430
                     /locus_tag="ACHXOL_004574"
     CDS             <1..>430
                     /locus_tag="ACHXOL_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR6811335.1"
                     /translation="KFPLTELNNPAHDSSAMSEKSHGREEIRLHIVCDVPDELIDFTF
                     EWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYISSADLTAEKFATAIRNHWHVENNLH
                     WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINVLTNDKVF"
BASE COUNT          144 a           73 c          102 g          111 t
ORIGIN      
        1 aaaaatttcc gctgacagaa ttaaataatc cagcgcatga cagttccgca atgagtgaaa
       61 agagtcacgg cagagaagaa atccgtcttc atattgtttg cgatgtccct gatgaactta
      121 ttgatttcac gtttgaatgg aaagggctga aaaaattatg cgtggcagtc tcctttcgct
      181 caataatagc agagcaaaag aaagagcccg aaatgatggt cagatattat atcagttctg
      241 ctgatttaac cgctgagaag tttgcgacag cgattcgaaa tcactggcac gtggagaata
      301 atctgcactg gcgtctggac gtggtaatga atgaagacga ctgcaaaata agaagaggaa
      361 atgcagcaga attattttca gggatacggc acattgctat taatgttttg acgaatgata
      421 aggtattcaa
//
LOCUS       DAZRUF010000613          420 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZRUF010000613 DAZRUF010000000
VERSION     DAZRUF010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.616"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..396)
                     /locus_tag="ACHXOL_004575"
     CDS             complement(<1..396)
                     /locus_tag="ACHXOL_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR6811336.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          104 a          117 c           77 g          122 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
//
LOCUS       DAZRUF010000614          420 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZRUF010000614 DAZRUF010000000
VERSION     DAZRUF010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.617"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHXOL_004576"
     tRNA            41..116
                     /locus_tag="ACHXOL_004576"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZRUF010000615          419 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZRUF010000615 DAZRUF010000000
VERSION     DAZRUF010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.618"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            121..419
                     /locus_tag="ACHXOL_004577"
                     /pseudo
     CDS             121..419
                     /locus_tag="ACHXOL_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          120 a           83 c          119 g           97 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtaa ataaagccct gagggtgatg gggtatgaca caacccagga tgtctgtggc
      181 catggattcc gggcgatggc gtgcagtgca ttgattgaat caggtttgtg gtcccgcgat
      241 gctgtggaac gtcagatgag ccatcaggag cgtaatggtg tacgtgctgc gtatatccat
      301 aaagcagaac atctggaaga acgtcgactg atgctacagt ggtgggcaga ttttctggat
      361 gcgaacagag ataagggtat cagcccgttt gaatatgcaa agattaacaa tccattaaa
//
LOCUS       DAZRUF010000616          418 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZRUF010000616 DAZRUF010000000
VERSION     DAZRUF010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.619"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>418
                     /locus_tag="ACHXOL_004578"
     CDS             <1..>418
                     /locus_tag="ACHXOL_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR6811337.1"
                     /translation="EAGRNMMAHIESSSYIKNNDGSEIMKFVMNNPEGERADLSKVEI
                     EITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDESSLHLITNKILACYQRE
                     ANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
BASE COUNT          142 a           68 c           91 g          117 t
ORIGIN      
        1 gaagcgggaa gaaatatgat ggctcatatt gagtcctcat cttatatcaa aaataatgat
       61 ggcagtgaga taatgaagtt tgttatgaat aatcctgaag gggaacgagc ggatttatct
      121 aaggtggaaa tagaaatcac actttccgct ttcactacta tggggactcg tcaaggacat
      181 acggctatta tatttcaaca acctgatggt tcgactaatc gttatgaagg aaagtccttt
      241 gaaagaaaag atgagagttc attacacctg attactaaca agattctggc atgttaccaa
      301 cgcgaagcta acaaggaaat agctcgtcta ttaaatattc cgcaggagtt aaataattct
      361 caggacttga ataactcgca ggtgagttgt aaagattcag ttgattctac gattacgg
//
LOCUS       DAZRUF010000617          409 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZRUF010000617 DAZRUF010000000
VERSION     DAZRUF010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.621"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..69
                     /locus_tag="ACHXOL_004579"
     CDS             <1..69
                     /locus_tag="ACHXOL_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811338.1"
                     /translation="GGTDEIMIYVAGRQILKDYQNK"
     gene            complement(112..>409)
                     /locus_tag="ACHXOL_004580"
     CDS             complement(112..>409)
                     /locus_tag="ACHXOL_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR6811339.1"
                     /translation="FRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQA
                     EISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
BASE COUNT          128 a           85 c           91 g          105 t
ORIGIN      
        1 ggcggtacag acgaaattat gatttacgta gcaggtcggc agatcctgaa agactatcag
       61 aacaaataat ctgcaggcgg cgcagctttt taacaaagtg cgccgccaga tttatccggc
      121 aagacttacc ggttgaggaa attcagataa gatttcttgt tccgcaaaac aatttttaaa
      181 ttgcctacgg taatcgctgg gcgaacaacc gacatggcgc aaaaacagtt tggcaaagtg
      241 atcgacattt tcataaccca cacgccagga gatctctgcc tgtgataatt cagtattagt
      301 gagtgaccat tttgcttccg tcatacgccg ttgaataaca tagttaatag gggaaatacg
      361 atactctttg gtaaattcat ggcaaatata actgacgctg gcacgaaat
//
LOCUS       DAZRUF010000618          406 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZRUF010000618 DAZRUF010000000
VERSION     DAZRUF010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.622"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>406)
                     /locus_tag="ACHXOL_004581"
     CDS             complement(<1..>406)
                     /locus_tag="ACHXOL_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811340.1"
                     /translation="SVYTNAYRGPMETGKIALCSAFIFLVLFAKNELRTKIKFGKLIL
                     FASLATQLLFFAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASILILKSDFRH
                     KTTLYTINFMLSLCAVIVTETRAAILVFPFFA"
BASE COUNT          144 a           67 c           86 g          109 t
ORIGIN      
        1 aacgcaaaga atggaaacac taatatggct gcacgcgttt cagttactat gacagcacat
       61 aagctaagca tgaagttaat tgtatataat gttgttttat gtctaaagtc ggattttaaa
      121 atgagaattg atgccagtaa agaagggaaa aggatgatgt agcctgctgt tgtagcgtgg
      181 gaagctgata atgcaacacg gtcaacgttt aaatagaaat gttgccacat ggcatgcgca
      241 aaaaaaagta actgcgttgc cagggatgcg aacagaatta gtttcccaaa ttttattttt
      301 gttctcaact catttttagc aaaaagaacc aagaaaataa aagcactaca caaggcaatt
      361 ttcccagttt ccattgggcc acgatatgca ttggtataaa cagagc
//
LOCUS       DAZRUF010000619          402 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZRUF010000619 DAZRUF010000000
VERSION     DAZRUF010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.623"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(105..>402)
                     /locus_tag="ACHXOL_004582"
     CDS             complement(105..>402)
                     /locus_tag="ACHXOL_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HFR6811341.1"
                     /translation="ALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLIS
                     IPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT          122 a          108 c           94 g           78 t
ORIGIN      
        1 caatatgtgg cttagctcat gaaaggagcg caacaagatg atgaatcaac atataacaac
       61 atcttaaaaa aaggcctgac attacgccag gccttctgcg ttaattaatc acgcttttcc
      121 agcagggtcc ggtaaatcag accaccgata atgccgccga caattggcac cacccagaag
      181 aaccacagtt gttctaatgc ccagccgccc tggaagatag caaccgcggt gctgcgcgcc
      241 gggttaacag aagtgttagt caccggaata ctaattaagt gaatcagggt taaggccaga
      301 ccaatagcga tcggcgcaaa acctgccggc gcgaatttgt cggttgcgcc gtggatcacc
      361 aacaggaaac ctgcactcaa taccagttca actaccagcg cg
//
LOCUS       DAZRUF010000620          400 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZRUF010000620 DAZRUF010000000
VERSION     DAZRUF010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.624"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            2..>400
                     /locus_tag="ACHXOL_004583"
     CDS             2..>400
                     /locus_tag="ACHXOL_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR6811342.1"
                     /translation="MSIEVLLQKNSELQIVLKTDDYRITIDYLRTRPVDLIIMDIDLP
                     GTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQ
                     MILSGYTFFPSETLNYIKSNKCSTNSSTVTV"
BASE COUNT          127 a           78 c           77 g          118 t
ORIGIN      
        1 aatgtctatt gaagttctgt tgcaaaaaaa cagtgaattg cagattgtcc tgaaaacgga
       61 tgattaccgc ataaccatcg attatctccg aacccgtcct gttgatttaa tcattatgga
      121 tatagacttg cccggaacag acggttttac cttcctgaaa aggatcaaac aaatccagag
      181 cacagtgaaa gtgttatttt tatcatcgaa atcagaatgc ttttatgctg gcagagcgat
      241 acaagctggt gctaacggtt ttgtcagtaa atgcaatgat cagaatgata tttttcatcc
      301 cgttcagatg atcctctccg gatacacgtt ttttcctagc gaaacgctca actatataaa
      361 aagcaataaa tgtagtacga atagttcaac ggtcactgtg
//
LOCUS       DAZRUF010000621          399 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZRUF010000621 DAZRUF010000000
VERSION     DAZRUF010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.625"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..298)
                     /locus_tag="ACHXOL_004584"
     CDS             complement(<1..298)
                     /locus_tag="ACHXOL_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811343.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYL"
BASE COUNT          138 a           88 c           60 g          113 t
ORIGIN      
        1 ttaaataact gagttcaccg cgagtataat ccattatttt tctggcccat gaaaactcaa
       61 gatcgtcacc ctccagatca gcataagaat aaaatgtagg ctcatgtgta ataatgatat
      121 tgacaccttc aaaaacagcc tttcttaaaa catccattga ggcaatccag caggtcccaa
      181 ttttattaat aagtgtcgcc tcagcaccaa aaataatttt atcaacagtt ggttgcgaaa
      241 cagcaaccat tgaatttaaa aaacaattaa gatccgcggc tgttttaata tgatgcattc
      301 aacatccttc acaggtttca tcagcaataa aataatttca acatttctgg gaaacgtttt
      361 gcccatgcag actcagagtg ggtatcacct ccccataaa
//
LOCUS       DAZRUF010000622          399 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZRUF010000622 DAZRUF010000000
VERSION     DAZRUF010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.626"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(80..>399)
                     /gene="tabA"
                     /locus_tag="ACHXOL_004585"
     CDS             complement(80..>399)
                     /gene="tabA"
                     /locus_tag="ACHXOL_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR6811344.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          108 a          108 c           81 g          102 t
ORIGIN      
        1 tttactcaca aaaaaatatc aaaaaacgct cttttcacaa actcgccgta aacctggagt
       61 tatttacggc gaaaagtcat cacgccatca gcatcttcac tactgctttg cgaacctggg
      121 ctggtgcacc cactgcgcac agcggtttat gcacttcccc cggataaaac acaacaaaat
      181 caccttcatt aaggataact gttttctcat caacgccttc cggcaaaaat gcgatgtctt
      241 tatcagctaa ccagtcggta tccggcgtgc ctgcaggttg cgtgctgaag gtcatgcctt
      301 cctgaccttt taacacaatc tgaatgtcga gatagcgggc atggtactcc gtacggcgag
      361 cttcgtgcgg ctcggtcata tcttccgaga taagataaa
//
LOCUS       DAZRUF010000623          389 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZRUF010000623 DAZRUF010000000
VERSION     DAZRUF010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.627"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>389)
                     /locus_tag="ACHXOL_004586"
     CDS             complement(<1..>389)
                     /locus_tag="ACHXOL_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR6811345.1"
                     /translation="KMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLW
                     LVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEV
                     LPAPLPPYRVLTGLVDRFGRTQTFHRE"
BASE COUNT           71 a          134 c          112 g           72 t
ORIGIN      
        1 ttcgcggtgg aacgtctgtg tgcgcccgaa gcggtccacc agcccggtca gtacccggta
       61 cggcggcagc ggcgcaggca gcacctcatc cgcttccggc acccgctcac accagccgag
      121 cagccaccac ggcccctgcg gactgtttgt cgccagataa cgatgcggac ttaagcggag
      181 ttcttccggc agcgcctgcc agagtgcggc cagccggtga ccttcatcca gtttcgccac
      241 gccgccgcgc accagccaga gtgattcgct gcggctgtaa ccgtcctcac cgggaaacag
      301 gtgctcaaaa tacaggcttc tgccgccgtt atcactgagt atcagtgtgt tatcgcgcag
      361 ctgtaagcgg atatccgcag gcattttcc
//
LOCUS       DAZRUF010000624          388 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZRUF010000624 DAZRUF010000000
VERSION     DAZRUF010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.628"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(101..313)
                     /gene="cspH"
                     /locus_tag="ACHXOL_004587"
     CDS             complement(101..313)
                     /gene="cspH"
                     /locus_tag="ACHXOL_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR6811346.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT          116 a           78 c           83 g          111 t
ORIGIN      
        1 attagtttct ttgtggaaaa gtaactgatg ttataatgta tgatgttgac tattgcacta
       61 ttccaataaa accagtcagc tttaaacaag cagcgtcata ttaagagaga taaacatttg
      121 ccgctgttgg tcctcgcagg ccatttacgc ggcaaaattc cacacgtaat cctggtataa
      181 gcacttctgc gtcgcgggga gtgaatgcgg aaatatggac ctgaacttct ttacgaccgt
      241 cggaggggat aatgaatcct ttgccgcttt tgcgatcaaa ggttttgaca attcctgtca
      301 ttttacggga caaacaaatt ccttactgaa aatactgcgc tgcactatac ggggttaata
      361 aaataaagcc agcgatattt aagaccgc
//
LOCUS       DAZRUF010000625          380 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZRUF010000625 DAZRUF010000000
VERSION     DAZRUF010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.629"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>380
                     /locus_tag="ACHXOL_004588"
     CDS             <1..>380
                     /locus_tag="ACHXOL_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFR6811347.1"
                     /translation="HDNETELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVAS
                     DVNDPATTCTCALYGFQFQGAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQS
                     KNSQTSSLWDAFAYTLAILYYENF"
BASE COUNT          106 a           79 c           93 g          102 t
ORIGIN      
        1 tcatgacaat gaaaccgagt tgatttacgt caagaaaggg gtagcaaggt ttaccatcga
       61 ttcttcgtta tatgtcgcgc acgccgatga cattgtggtg atagaacgtg gcaggctgca
      121 tgcggtggcc tctgacgtta acgatccagc aacgacgtgt acctgtgcgc tgtacggctt
      181 tcagtttcag ggggctgagg aaaatcagct actgcaaccg cattcttgcc cggtaattgc
      241 cgcagggcag ggaaaagaag tcattaaaac cttatttaat gagctaagtg tgattttgcc
      301 gcaaagtaaa aatagccaaa catcttcgtt atgggatgca tttgcctata cattagcaat
      361 tctttactac gaaaacttta
//
LOCUS       DAZRUF010000626          368 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZRUF010000626 DAZRUF010000000
VERSION     DAZRUF010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.630"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>368
                     /locus_tag="ACHXOL_004589"
     CDS             <1..>368
                     /locus_tag="ACHXOL_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR6811348.1"
                     /translation="ARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYI
                     DSHLHNDLIDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDV
                     ILFSKPVPTAVFVTIILLCA"
BASE COUNT          111 a           70 c           66 g          121 t
ORIGIN      
        1 tgcccgtctg gcaatgtatg aagttggctt aaaaacatat tctccaatag gacaatcact
       61 ggaaaaacga gcggaaaaaa tacatgaact agaagaaaaa gagcctagat tgagtggcgc
      121 tttaccctat atagattctc atttgcataa cgatctcata gatacgttat caacgcgtgg
      181 tattcctgga gttgtattaa caattttagc cttttcagca atactcatat atgccttaag
      241 aactgctaaa gaaccttata ttttaatctt gcttttttca ctactggtag taggcctaag
      301 tgatgtaata ctcttttcta aaccggttcc gactgctgtg tttgtcacca taatattgct
      361 ttgtgctt
//
LOCUS       DAZRUF010000627          360 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZRUF010000627 DAZRUF010000000
VERSION     DAZRUF010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.632"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(16..285)
                     /locus_tag="ACHXOL_004590"
     CDS             complement(16..285)
                     /locus_tag="ACHXOL_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR6811349.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
BASE COUNT          103 a           91 c           62 g          104 t
ORIGIN      
        1 gcataatccc aatgattact ccctgacagg atttgcaggc cactcaatat caggtgcagt
       61 tgatgtatca acacgattca acaatacccg atatttattc catgcctcca gcaacgatct
      121 ttcttcctcc gttgcgattt ccagatctac agcatcctgc agtggcgcaa tatactcact
      181 aaattcctgg atgtagaact gtgtggtgac ggtcttccag ccattcggct cctgctgtat
      241 cgaagcatac caggctattt caatatcgct atgctgcggc agcatttaac cccttgtaat
      301 tcatcgccat aattgattta attcacaaat aaaactataa catggtgaaa tcaatgaaaa
//
LOCUS       DAZRUF010000628          359 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZRUF010000628 DAZRUF010000000
VERSION     DAZRUF010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.633"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            33..>359
                     /locus_tag="ACHXOL_004591"
     CDS             33..>359
                     /locus_tag="ACHXOL_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811350.1"
                     /translation="MSITTIAATYKDVLTIAFSALSFIIACTSLYFSGRAAWHDRSRL
                     KITADLVYESFDEEPYKIEVIVINVGRRDAVLEGMLCHYEKGIRSHSSPKESIVIKEK
                     QRKKFEL"
BASE COUNT          122 a           52 c           79 g          106 t
ORIGIN      
        1 gctttaaatt agacatgtaa gttgagagaa aaatgagcat aacaacaata gcagctacat
       61 ataaagatgt gttaactatt gctttttcgg cgttgtcatt tattattgca tgtacttcct
      121 tatacttctc cggtagggca gcatggcatg atagatcaag gctaaaaata acagctgacc
      181 tcgtctacga gtcttttgat gaggaacctt ataaaatcga ggtgatagtg atcaatgttg
      241 gtaggcgtga tgcagttttg gaagggatgc tttgccatta tgaaaaaggc attaggagcc
      301 attcatctcc aaaagagagc attgttatta aagaaaagca aagaaaaaaa tttgaattg
//
LOCUS       DAZRUF010000629          357 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZRUF010000629 DAZRUF010000000
VERSION     DAZRUF010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.634"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>357
                     /locus_tag="ACHXOL_004592"
     rRNA            <1..>357
                     /locus_tag="ACHXOL_004592"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           95 a           82 c          105 g           75 t
ORIGIN      
        1 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
       61 gccggaaggt taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa
      121 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
      181 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
      241 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
      301 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtg
//
LOCUS       DAZRUF010000630          354 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZRUF010000630 DAZRUF010000000
VERSION     DAZRUF010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.635"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
BASE COUNT          124 a           61 c           73 g           96 t
ORIGIN      
        1 agagcattat tctatgattt acacagggaa tataggggga ttatggcaca cacatagaaa
       61 gacattacac agagtgctat gtgacaaatg gatgtaacga gaggttacat tcatttatct
      121 gaaatgctct ctgtaagaaa gttggtagta acgatatgtt acatagacta attattgaat
      181 gtaacgatat tacacatcgg tagtaacgat acgttacatc ggaagatgat tggatgtaac
      241 ggtagctcac agtgaactag gggtaggtca tagtgaacta cccccacaga attctgttac
      301 ccctatcaag tctgataccc ctatcaaaac tgatacccga taagtataaa taac
//
LOCUS       DAZRUF010000631          354 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZRUF010000631 DAZRUF010000000
VERSION     DAZRUF010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.636"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..168)
                     /locus_tag="ACHXOL_004593"
     CDS             complement(<1..168)
                     /locus_tag="ACHXOL_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR6811351.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          118 a           80 c           68 g           88 t
ORIGIN      
        1 atcgataacg tctcccactg agataagtaa atctcgccac ggatcaaaac gacaatgcca
       61 taatttgcgg cgcaactgct caagacaacc gtgtatatcg ccagaaagcc agatatgtcg
      121 ccattgatga cccgcaattc tctgataaac gggcgcaggc tgtttcatca atattttcct
      181 cccgcgctaa agatcacata attttaacaa gaatgttaaa aaacgttgga ctcagacagt
      241 agagtgtgtg ttatggttga ctataaagtc agcgaaggaa atgcttctgg cttttaacag
      301 ataaaaagag accgaacacg attcctgttt tcgtcaacaa acaataaaac cctt
//
LOCUS       DAZRUF010000632          349 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZRUF010000632 DAZRUF010000000
VERSION     DAZRUF010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.637"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(47..296)
                     /gene="ymgC"
                     /locus_tag="ACHXOL_004594"
                     /pseudo
     CDS             complement(47..296)
                     /gene="ymgC"
                     /locus_tag="ACHXOL_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
BASE COUNT          117 a           64 c           61 g          107 t
ORIGIN      
        1 ttaagtgata ctggttatct ggagattcag ggggccagtc taagttctaa gagagcacgg
       61 attccctgtc attaataatg atatccgtta gtgtagtctt atgcatttca atgtactgca
      121 atatttgcag atagagatca gaaagaatca cactggtttc atcttttaaa cgcaagcgta
      181 aagcctgatc aataatatga ctatcaacat acccatgcgt catgaacacc tccctctcga
      241 gatttttaaa caaagcacat tgaaaaaata aatctctctg ggattgaatt attcatgtca
      301 gcctcgtaga aatattaacc taataaaatt atgctgtgta tagtctaaa
//
LOCUS       DAZRUF010000633          346 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZRUF010000633 DAZRUF010000000
VERSION     DAZRUF010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.638"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            106..306
                     /gene="glgS"
                     /locus_tag="ACHXOL_004595"
     CDS             106..306
                     /gene="glgS"
                     /locus_tag="ACHXOL_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR6811352.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT           97 a           61 c           88 g          100 t
ORIGIN      
        1 atatttacgc acgttatgtt taaaggcact acactgattg gggaatactg aaatcagaaa
       61 aagcatatgg ccttaaaagg tatgcgataa ccggaggatg tgcttatgga tcatagtctt
      121 aattctttaa ataatttcga tttcctggcg cgtagttttg ccagaatgca cgcagaaggt
      181 cgcccggtcg atattcttgc cgttactggt aacatggatg aagaacatag aacctggttt
      241 tgcgcacgtt atgcctggta ttgccaacag atgatgcagg caagagagct ggagttagag
      301 cactgatata acgggcctga tggcccgttt tagtgtttgt attaga
//
LOCUS       DAZRUF010000634          344 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZRUF010000634 DAZRUF010000000
VERSION     DAZRUF010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.639"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>344
                     /locus_tag="ACHXOL_004596"
     CDS             <1..>344
                     /locus_tag="ACHXOL_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR6811353.1"
                     /translation="VPGGTWKDWPKELVAACHMKSSGKGYASVYGRMSWDKPSPTITT
                     LCYGFGNGRFGHPEQHRAISLREAALLQTFPMDYIFVEDKNKFVIRSIGKMIGNAVPV
                     ELGKAIGQSIKN"
BASE COUNT          101 a           54 c           90 g           99 t
ORIGIN      
        1 ctgttcctgg tggtacatgg aaggactggc cgaaagagtt ggttgccgct tgtcatatga
       61 aatccagcgg taaagggtat gctagtgtat atgggcgcat gtcttgggat aagccaagtc
      121 caacaattac aactctttgt tatggtttcg ggaatgggcg ttttggacat ccagaacagc
      181 atagagcgat ttctcttaga gaggctgctt tattgcaaac tttcccgatg gattatattt
      241 ttgtagaaga caaaaacaag tttgttataa gaagtattgg gaaaatgata ggtaatgctg
      301 ttcctgtgga actaggaaaa gcaattgggc aatcaatcaa gaac
//
LOCUS       DAZRUF010000635          342 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZRUF010000635 DAZRUF010000000
VERSION     DAZRUF010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.640"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(37..189)
                     /gene="hokA"
                     /locus_tag="ACHXOL_004597"
     CDS             complement(37..189)
                     /gene="hokA"
                     /locus_tag="ACHXOL_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR6811354.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
BASE COUNT          100 a           78 c           68 g           96 t
ORIGIN      
        1 gtaagaaagg cgggaataac ttcccgccaa agactcttac tctttcaaat tgcaggctaa
       61 aaatgccgcc agctcataac tctcctgttt aatatgcaat tcacacagtg aatctcttat
      121 catccaggtg aaaaataaaa gcgtgaaaca aatcactatt aaagaaagta atctatattt
      181 ctgcggcatt cccagcctcc tgtgttgatt tccaacgagt aagaggctaa ccttatggtg
      241 ttcaggcata gggtagcctc actttgattt atagtcaggt ggggcttttc tctgtctgcc
      301 tttcggtgaa tacctgagac aaacagtctc aagcacccgt gg
//
LOCUS       DAZRUF010000636          336 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZRUF010000636 DAZRUF010000000
VERSION     DAZRUF010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.641"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
BASE COUNT          103 a           61 c           72 g          100 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaata
//
LOCUS       DAZRUF010000637          333 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZRUF010000637 DAZRUF010000000
VERSION     DAZRUF010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.642"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(94..>333)
                     /locus_tag="ACHXOL_004598"
     CDS             complement(94..>333)
                     /locus_tag="ACHXOL_004598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811355.1"
                     /translation="VRGLNADKNKISAIRQCNYKMRNYLGVGFHLFCEPDDIEFMEKY
                     VTEELKGSYAVFKLCECPVRYNCQCEFNEIGVVLF"
BASE COUNT          103 a           73 c           53 g          104 t
ORIGIN      
        1 atgggtccct ccatactggc ggtactatcg agggttttcg gcagcgggtt taatcaattg
       61 tgtgtgaaca aaatgatcat acccgcaccg catttaaaaa agtaccacac caatttcatt
      121 aaattcacat tggcaattat atcttactgg acactcgcat aatttaaaca cggcgtaaga
      181 gcctttaagt tcttcagtta cgtacttttc cataaactcg atatcatctg gttcacaaaa
      241 aagatgaaat ccgacaccaa gataattacg cattttgtag ttacactgtc ttatagcact
      301 aattttattt ttgtcagcat ttaagcccct aac
//
LOCUS       DAZRUF010000638          332 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZRUF010000638 DAZRUF010000000
VERSION     DAZRUF010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.643"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..332
                     /locus_tag="ACHXOL_004599"
                     /pseudo
     CDS             1..332
                     /locus_tag="ACHXOL_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           85 a           83 c           99 g           65 t
ORIGIN      
        1 gctatgcagc gaactgcata ttgccccgtc aacgtattac cactgtcagc aacagcgaca
       61 tcatccggat aaacgcagtg cccgtgcgca gcgcgatgac tggctgaaga aagagataca
      121 gcgcgtatac gatgaaaatc acaaggtata cggtgtaaag tctggcgtca gttgttacgg
      181 gaaggtatca gagtggccag atgcactgtg gcacgtctca tggcggttat gggacttgcc
      241 ggtgttctcc ggggtaaaaa ggtccgtacg accatcagcc ggaaagccgt tgccgcaggc
      301 gaccgcgtaa accgtcagtt cgtggcagaa cg
//
LOCUS       DAZRUF010000639          328 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZRUF010000639 DAZRUF010000000
VERSION     DAZRUF010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.644"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..305)
                     /locus_tag="ACHXOL_004600"
     CDS             complement(<1..305)
                     /locus_tag="ACHXOL_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR6811356.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVTQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT           98 a           70 c           69 g           91 t
ORIGIN      
        1 cttgaatcct ctctcggcac ggttgcaatt agcgagtggg tagtatcacc aacagcgcga
       61 aatccgcgtc tgataatgga tatgttctga tataacgtgt tagtaggcac acgcatacct
      121 tcttcttccc agactctggt gaaaaaatct tgttgtgtaa ccacatcagg aaaagcttca
      181 agtagaagtg ataaacatcg actggctggc gttgtcagaa cgacgttaag atcggcgtta
      241 tgtaccgata ttaattttcg gtgctcaggc cagaactcga tattatcgtt aataatccag
      301 tacatacgtc aataaacact aaatcaat
//
LOCUS       DAZRUF010000640          327 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZRUF010000640 DAZRUF010000000
VERSION     DAZRUF010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.645"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(167..>327)
                     /locus_tag="ACHXOL_004601"
     CDS             complement(167..>327)
                     /locus_tag="ACHXOL_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR6811357.1"
                     /translation="ELSFYEYRKNLATAGVFRWITNIHENKRYYYTFDNSLLFTENIQ
                     NTTQIFPH"
BASE COUNT           88 a           67 c           76 g           96 t
ORIGIN      
        1 cgtcgccgta ttcccgtatt aacagagacc gtagcccgac ggggaactcc ttctgcgcga
       61 gtgtgcggga ataatcaaaa acgatgcaca ccggggttac cgggtacaca tatttcatca
      121 tgccagcgag tccggttctg gcacggaaga aaccggacgt tatgatttag tgcggaaata
      181 tttgtgtagt gttctgtatg ttctcagtaa agagtaatga attatcaaag gtatagtaat
      241 accttttgtt ttcgtggata tttgtaatcc atctgaaaac ccctgctgta gcaagatttt
      301 tcctgtattc gtaaaatgat aactctc
//
LOCUS       DAZRUF010000641          326 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZRUF010000641 DAZRUF010000000
VERSION     DAZRUF010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.646"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            79..258
                     /locus_tag="ACHXOL_004602"
                     /pseudo
     CDS             79..258
                     /locus_tag="ACHXOL_004602"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            206..>326
                     /locus_tag="ACHXOL_004603"
     CDS             206..>326
                     /locus_tag="ACHXOL_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFR6811358.1"
                     /translation="MVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQLLDET"
BASE COUNT           92 a           86 c           84 g           64 t
ORIGIN      
        1 tcatgggctg ttaatcatta ccgtgataac gccattacct acaaagagca gcgcgacaaa
       61 aacgcaagag aactgaagct ggcgaacgca accattactg acatgcagca gcgccagcgt
      121 gatgctgatg cactcgatgc taaatacacg aaggagttag ctgatgcgaa agctgaaaat
      181 gatgctcttc ggcgcaagct tgataatggt ggtcgggtgc tcgtcaaagg aaaatgccct
      241 gtgccatcct cagccgaaac ctccagcgcc tccggcatgg gcaatgatgc caccgtcgaa
      301 ctctctccag ttgctggacg aaacgt
//
LOCUS       DAZRUF010000642          320 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZRUF010000642 DAZRUF010000000
VERSION     DAZRUF010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.647"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
BASE COUNT           76 a           88 c           67 g           89 t
ORIGIN      
        1 ttaacgtacg ttttcgttcc attggccctc accccccgat aacgacccag gtagcgcgcc
       61 gtattccccc cgccggccgt tttcttcgac atgtacatca ggcgcggaac tcatcggggc
      121 gcgggaatga aatcccattc agactccgga tagattcaag caagcccact tatcgtcaaa
      181 atcggtgttg caaaaacggg agtgaccata gatccgtttt ccaagcggac cccataatat
      241 cggctggtaa acatcactcg ctagtgcatt ctttttgttg gtaatgataa tcatttacat
      301 tcattctttg atgttttatc
//
LOCUS       DAZRUF010000643          318 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZRUF010000643 DAZRUF010000000
VERSION     DAZRUF010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.648"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..>318
                     /locus_tag="ACHXOL_004604"
     CDS             <1..>318
                     /locus_tag="ACHXOL_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR6811359.1"
                     /translation="WARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYAS"
BASE COUNT           90 a           77 c           90 g           61 t
ORIGIN      
        1 tgggcccgtc gaccgtccgg cacggtccat cacagtgata aaggttctca gtatgtatcg
       61 ctggcctaca cacagcggct taaggaagcc ggattactgg catcaacagg aagtacaggc
      121 gactcgtatg acaacgcgat ggcggagagc atcaatggtc tttacaaagc ggaggtaata
      181 caccgtaaga gctggaaaaa ccgtgcagaa gtggaactgg ccacactcac gtgggtggac
      241 tggtataaca atcgacgatt gctggaaagg ctgggccata ctcctccggc agaagcagaa
      301 aaagcttatt atgcttcc
//
LOCUS       DAZRUF010000644          316 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZRUF010000644 DAZRUF010000000
VERSION     DAZRUF010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.649"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            81..>316
                     /locus_tag="ACHXOL_004605"
     CDS             81..>316
                     /locus_tag="ACHXOL_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR6811360.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVY"
BASE COUNT           82 a           65 c           85 g           84 t
ORIGIN      
        1 gataaatcct catataacgc cattgaatgg tccgtctctc cggaaggttc ttcctgattt
       61 aatttatcgc gagaaaaact atgtctgtag tgatatcagg tgcgctgact gatggcgcag
      121 gcatccccat gtccggatgc cagattattc tgaaatcccg tgtaaacacc tcagaagtgg
      181 tgatgcgtac cagggctgat gtagtgaccg gaaataacgg tgaatattcg tttgaggcac
      241 aggtcggaaa atattgcgtg tatctgaaac gggactggcg cgacgagtac tgtgttggcg
      301 acattgctgt atacga
//
LOCUS       DAZRUF010000645          310 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZRUF010000645 DAZRUF010000000
VERSION     DAZRUF010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.650"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            20..229
                     /gene="fumD"
                     /locus_tag="ACHXOL_004606"
     CDS             20..229
                     /gene="fumD"
                     /locus_tag="ACHXOL_004606"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR6811361.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          100 a           55 c           81 g           74 t
ORIGIN      
        1 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
       61 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
      121 tattgttatc gcaggcgtat tacgtactgc attagccaat aaacgcattc agcgcagtga
      181 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
      241 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
      301 ataacaactg
//
LOCUS       DAZRUF010000646          306 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZRUF010000646 DAZRUF010000000
VERSION     DAZRUF010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.652"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
BASE COUNT           75 a           80 c           54 g           97 t
ORIGIN      
        1 aacactgtgt tccttcccca ggtgattacc ctggggttat tttctgaaag tttcctttgc
       61 aaaaggatag cgttgtaaat tactttaacg aattactcca tgaactcatt aaagttcttt
      121 cgtaatactt tatcgtcaaa cacgttatag ctggcatcaa aaaccacaga tttaccctgc
      181 cagcgggtaa tcccctcctc tggcgttttc ggttgaactg gtatccccgt ggatttgccc
      241 ctatatttcc agacatctgt tatcacttaa cccattacaa gcccgctgcc gcagatattc
      301 ccgtgg
//
LOCUS       DAZRUF010000647          306 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZRUF010000647 DAZRUF010000000
VERSION     DAZRUF010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.653"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
BASE COUNT          114 a           44 c           66 g           82 t
ORIGIN      
        1 agtctatttt ataaaaaaat taagtaggct aagaagtacc ttagaaatca attttaaggg
       61 gtctaaaacg ctctgtaacg agctaaactg tataagtggc ataagatgcc ttttagactg
      121 acactcgctt agagagcttc ctagagcttc tgggaggatt cattagatat cgttgtatac
      181 agagacacag tatctataaa gggtattaag agattcttag aagttactgt aaagaaccac
      241 taagataaga agagagggac aaagaaccga aggaaagtcc cttattaagt aggagtctta
      301 atagac
//
LOCUS       DAZRUF010000648          303 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZRUF010000648 DAZRUF010000000
VERSION     DAZRUF010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.denovo.654"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            complement(<1..>303)
                     /locus_tag="ACHXOL_004607"
     CDS             complement(<1..>303)
                     /locus_tag="ACHXOL_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR6811362.1"
                     /translation="NYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINT
                     SAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDRQHGFAPVIHGI"
BASE COUNT           86 a           71 c           57 g           89 t
ORIGIN      
        1 caataccatg aatcaccgga gcgaaaccat gttgtctatc gggcaacatc gcttctgttg
       61 aatttatttt gaggccacga aagttcacac tatcaaaaat atcgccatcg gtataactat
      121 cacctaccgt taaccgtgag cgtaagggaa taatgtcacg ttcagccgac gtattgatat
      181 gctgccattt attgctgtca gatgaattgc tactcccact gttataactc cacgttgagt
      241 tatcgcgtag tcgccaacta ccaatgttga tgccactctg taaattcaaa tatgcatagt
      301 ttg
//
LOCUS       DAZRUF010000649         8146 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.guided.183, whole genome shotgun sequence.
ACCESSION   DAZRUF010000649 DAZRUF010000000
VERSION     DAZRUF010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.guided.183"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            207..2015
                     /gene="frdA"
                     /locus_tag="ACHXOL_004608"
     CDS             207..2015
                     /gene="frdA"
                     /locus_tag="ACHXOL_004608"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR6811363.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            2008..2742
                     /gene="frdB"
                     /locus_tag="ACHXOL_004609"
     CDS             2008..2742
                     /gene="frdB"
                     /locus_tag="ACHXOL_004609"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR6811364.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2753..3148
                     /gene="frdC"
                     /locus_tag="ACHXOL_004610"
     CDS             2753..3148
                     /gene="frdC"
                     /locus_tag="ACHXOL_004610"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR6811365.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3159..3518
                     /gene="frdD"
                     /locus_tag="ACHXOL_004611"
     CDS             3159..3518
                     /gene="frdD"
                     /locus_tag="ACHXOL_004611"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR6811366.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            3581..4714
                     /locus_tag="ACHXOL_004612"
     CDS             3581..4714
                     /locus_tag="ACHXOL_004612"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR6811367.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            4803..5336
                     /gene="blc"
                     /locus_tag="ACHXOL_004613"
     CDS             4803..5336
                     /gene="blc"
                     /locus_tag="ACHXOL_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR6811368.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5333..5650)
                     /gene="sugE"
                     /locus_tag="ACHXOL_004614"
     CDS             complement(5333..5650)
                     /gene="sugE"
                     /locus_tag="ACHXOL_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR6811369.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(5825..5971)
                     /gene="ecnB"
                     /locus_tag="ACHXOL_004615"
     CDS             complement(5825..5971)
                     /gene="ecnB"
                     /locus_tag="ACHXOL_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR6811370.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(6082..6207)
                     /gene="ecnA"
                     /locus_tag="ACHXOL_004616"
     CDS             complement(6082..6207)
                     /gene="ecnA"
                     /locus_tag="ACHXOL_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR6811371.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(6259..6825)
                     /gene="efp"
                     /locus_tag="ACHXOL_004617"
     CDS             complement(6259..6825)
                     /gene="efp"
                     /locus_tag="ACHXOL_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR6811372.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            6867..7895
                     /gene="epmB"
                     /locus_tag="ACHXOL_004618"
     CDS             6867..7895
                     /gene="epmB"
                     /locus_tag="ACHXOL_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR6811373.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2027 a         2093 c         2128 g         1898 t
ORIGIN      
        1 gtttagtaat taaattaatc atcttcagtg ataatttagc cctcttgcgc actaaaaaaa
       61 tcgatctcgt caaatttcag acttatccat cagactatac tgttgtacct ataaaggagc
      121 agtggaatag cgttcgcaga ccgtaacttt caggcactta ccctgaagta cggggctgtg
      181 ggataaaaac aatctggagg aatgtcgtgc aaacctttca agccgatctt gccattgtag
      241 gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc gcaggcaaat ccaaatgcaa
      301 aaatcgcact aatctcaaaa gtatacccga tgcgtagcca taccgttgct gcagaagggg
      361 gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata tcactttcac gatacagtag
      421 cgggtggcga ctggttgtgt gagcaggatg tcgtggatta tttcgtccac cactgcccaa
      481 ccgaaatgac ccaactggaa ctgtgggggt gcccatggag ccgtcgcccg gatggtagcg
      541 tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgcac ctggttcgcc gccgataaga
      601 ccggcttcca tatgctgcac acgctgttcc agacctctct gcaattcccg cagatccagc
      661 gttttgacga acatttcgtg ctggatattc tggttgatga tggtcatgtt cgcggcctgg
      721 tagcaatgaa catgatggaa ggcacgctgg tgcagatccg tgctaacgcg gtcgttatgg
      781 ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa cggcggcatc gttaccggtg
      841 acggtatggg tatggcgcta agccacggcg ttccgctgcg tgacatggaa ttcgttcagt
      901 atcacccaac cggtctgcca ggttccggta tcctgatgac cgaaggctgc cgtggtgaag
      961 gcggtattct ggtcaacaaa aatggctacc gttatctgca agattacggc atgggcccgg
     1021 aaactccgct gggcgagccg aaaaacaaat atatggaact gggtccacgc gacaaagttt
     1081 ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat ctccacgccg cgtggcgatg
     1141 tggtttatct cgacttgcgt cacctcggcg agaaaaaact gcatgaacgt ctgccgttca
     1201 tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt taaagaaccg attccggtac
     1261 gtccgaccgc acactacacc atgggcggta tcgaaaccga tcagaactgt gaaacccgca
     1321 ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg tctgcacggt gcaaaccgtc
     1381 tgggctccaa ctccctggcg gaactggtgg tcttcggccg tctggccggt gaacaagcga
     1441 cagagcgtgc agcaactgcc ggtaatggca acgaagcggc aattgaagcg caggcagctg
     1501 gcgttgaaca acgtctgaaa gatctggtta accaggatgg cggcgaaaac tgggcgaaga
     1561 tccgcgacga aatgggcctg gcaatggaag aaggttgcgg tatctaccgt acgccggaac
     1621 tgatgcagaa aaccatcgac aagctggcag agctgcagga acgcttcaag cgcgtgcgca
     1681 tcaccgacac ctccagcgtg ttcaacaccg acctgctcta caccattgaa ctgggccacg
     1741 gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc acgtaaagag tcccgcggcg
     1801 cacaccagcg tctggacgaa ggttgcaccg agcgtgacga cgtcaacttc ctcaaacaca
     1861 ccctcgcctt ccgcgatgct gatggcacga ctcgcctaga gtacagcgac gtgaagatta
     1921 ctacgctgcc gccagctaaa cgcgtttacg gtggcgaagc ggatgcagcc gataaggcgg
     1981 aagcagccaa taagaaggag aaggcgaatg gctgagatga aaaacctgaa aattgaggtg
     2041 gtgcgctata acccggaagt cgataccgca ccgcatagcg cattctatga agtgccttat
     2101 gacgcaacta cctcattact ggatgcgctg ggctacatca aagacaacct ggcaccggac
     2161 ctgagttacc gctggtcctg ccgtatggcg atttgtggtt cctgcggcat gatggttaac
     2221 aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt acaccgacgg tatgaaggtt
     2281 gaagcgttag ctaacttccc gattgaacgc gatctggtgg tcgatatgac ccacttcatc
     2341 gaaagtctgg aagcgatcaa accgtacatc atcggcaact cccgcaccgc ggatcagggt
     2401 actaacatcc agaccccggc gcagatggca aagtatcacc agttctccgg ttgcatcaac
     2461 tgtggtctgt gctacgccgc gtgcccgcag tttggcctga acccagagtt catcggtccg
     2521 gctgccatta cgctggcgca tcgttataac gaagatagcc gcgaccacgg taagaaggag
     2581 cgtatggcgc agttgaacag ccagaacggc gtatggagct gtactttcgt gggctattgc
     2641 tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca ttcagcaggg caaagtagaa
     2701 agttcgaaag actttcttat cgcgaccctg aaaccacgct aaggagtgca acatgacgac
     2761 taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg tggaaaaaat tgccgtttta
     2821 tcgcttttac atgctgcgcg aaggcacggc ggttccggct gtgtggttca gcattgaact
     2881 gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc tgggcgggat tcgtcgactt
     2941 tttacaaaac ccggttatcg tgatcattaa cctgatcact ctggcggcag ctctgctgca
     3001 caccaaaacc tggtttgaac tggcaccgaa agcggccaat atcattgtaa aagacgaaaa
     3061 aatgggacca gagccaatta tcaaaagtct ctgggcggta actgtggttg ccaccatcgt
     3121 aatcctgttt gttgccctgt actggtaagg agcctgagat gattaatcca aatccaaagc
     3181 gttctgacga accggtattc tggggcctct tcggggccgg tggtatgtgg agcgcaatta
     3241 ttgcgccggt gatgatcctg ctggtgggta ttctgctgcc actggggctg tttccgggtg
     3301 atgcgctgag ctacgagcgc gttctggcgt tcgcgcagag cttcattggt cgcgtattcc
     3361 tgttcctgat gatcgttctg ccgctgtggt gtggtttaca ccgtatgcac cacgcgatgc
     3421 acgatctgaa aattcacgtg cctgcgggca aatgggtttt ctacggtctg gctgctatcc
     3481 tgacagttgt cacgctgatt ggtatcgtta caatctaacg tatcgccaat gtaaatccgg
     3541 cccgcctatg gcgggccgtt ttgtatggaa accagacctt atgttcaaaa cgacgctctg
     3601 cgccttatta attaccgcct cttgctccac atttgctgcc cctcaacaaa tcaacgatat
     3661 tgtgcatcgc acaattaccc cgcttataga gcaacaaaag atccccggta tggcggtggc
     3721 ggtaatttat cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa
     3781 gcagcccgtc acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttacggg
     3841 cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccgcaacaaa
     3901 atactggcct gaacttactg ctaaacagtg gaatgggatc acactattac atcttgcgac
     3961 ctacaccgct ggcggcctgc cattgcaggt gccggatgaa gtgaaatcct caagcgactt
     4021 gctgcgcttc tatcaaaact ggcagcctgc atgggctcca ggaacacaac gtctgtatgc
     4081 caactccagt atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga
     4141 gcaggcgatg caaactcgtg tcttccagcc actcaaactc aaccatacgt ggattaatgt
     4201 acctccccca gaagaaaaga attacgcctg gggatatcgc gaaggtaagg cagtgcatgt
     4261 ttcgccaggg gcgttagatg ctgaagctta tggtgtgaag tcgaccattg aagatatggc
     4321 ccgctgggtg caaagcaatt taacccccct tgatatcaat gagaaaacgc ttcaacaagg
     4381 gatacaactg gcacaatctc gctactggca aaccggcgat atgtatcagg gcctgggctg
     4441 ggaaatgctg gactggccgg tcaatcctga cagcatcatt aacggcagtg acaataaaat
     4501 tgcactggca gcacgcccgg taaaagcgat taagccccca actcctgcag tacgcgcatc
     4561 atgggtacat aaaacggggg cgaccggcgg atttggtagc tatgtcgcgt ttattccaga
     4621 aaaagagctg ggtatcgtga tgctggctaa caaaaactat cccaatccag cgagagtcgc
     4681 cgccgcctgt cagattctca acgctctaca gtaaaatttc atcgggtccg aattttcgga
     4741 ccttttctgc gcttttcctt gctgtcatct acacttagaa aaaaaccagt aaggaaacat
     4801 ttatgcgcct gctccctctc gttgccgcag cgacagctgc atttctggtc gttgcctgca
     4861 gttctcctac gccgccgcgt ggcgtgacag tagtgaataa tttcgacgct aaacgctatc
     4921 tcggtacctg gtatgagatt gcccgttttg atcaccgttt tgaacgtgga ctggaaaaag
     4981 tcaccgcaac atacagtctg cgtgatgacg gcggcctgaa tgtcatcaat aaaggctata
     5041 accctgacag agaaatgtgg cagcagagtg aagggaaagc gtactttacc ggcgcaccaa
     5101 cacgcgctgc gctgaaagtg tcattctttg gtcctttcta tggcggttat aacgtcattg
     5161 cacttgatcg ggaataccgc catgcgctgg tttgcggccc ggaccgcgac tacctgtgga
     5221 tactctcccg cacgccaacc atttctgacg aagtgaaaca ggagatgctg gcagtcgcga
     5281 cccgggaagg gtttgatgtc agtaagttta tttgggtaca gcagcccggt agttagtgag
     5341 tgctgagttt cagaccaata atccccaata cgattaacgc cagactcgcc aggcgcatcg
     5401 ggttagcgga ctcaccgagc agcacaatgc cggtgatggc cgcgccgact gcgccaatac
     5461 ccgtccacac ggcataagcc gtccctaccg gtaacgattt catcgcccag gcaagtagcg
     5521 ccatactgac tatcatcgcc gtcacagtaa taacacttgg cgtcaaacga ctaaagccgt
     5581 gggtatattt caggccaacg gcccatacca cttccagcag accagcaata actaagataa
     5641 tccaggacat atcaggctcc ggagaattgg ggccgtcccc ggtgaaaaga ggcgtttgca
     5701 ggtcgtcctg caaagctgat ggttgaacgg atattttgcc aggtggcagg ggaatttcaa
     5761 ctattacctt tgtgactatc gcaaaaaaac aaacggcacg acataactgt cgtaccgtta
     5821 ttgcttattg ctgcgctttc gttgctgcac cagaaatcgc gttaccgcca tcagaaatgt
     5881 cttcaccaac gccacgcgtg gtgttgcagg cagttaatac tgttgaaagc accagaacag
     5941 aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc cttttaggta atgttattta
     6001 tgtttgccta tagcaaatta ataatagaca acatcgccaa aaatgatgga aatctgatga
     6061 tttttcggaa gagaagaaaa attagctggc agcgcgggag atggagttgc cgagatgttt
     6121 gatgtcttcg ccgaaaccgc gagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa
     6181 aacaagaacg ataaggcgtt tcatcatctc tgacctgaat aagtgatggt gcagcctgca
     6241 ggccgcacca caaccgcatt acttcacgcg agagacgtat tcaccagagc gggtatccac
     6301 tttgatgact tcgccgattt gtacgaacag cggaacttta accacagcgc cagtagacag
     6361 ggtagccggt ttgccgccag tacctgcggt atcacctttc aggcccggat cggtatcaac
     6421 gatttccagt tcaacgaagt tcggcggagt aacggagatc ggctgaccat tccacagagt
     6481 tacgatacac tctgcctgat ccagcagcca tttagcgttg tcgccaattg cttttgcatc
     6541 agcagacagc tgctcgaaag tttcgttgtt catgaagtgc cagaactcac cgtcgttgta
     6601 caggtaagtc aggttcatat cgacaacatc agcgccttca gcggaatcag tagatttgaa
     6661 ggttttttct acgcgagtac cggtcagcag acgacgcagt ttaacgcgag caaatgcctg
     6721 gcctttaccc ggttttacga attcactcgc ttcaaccgcg taaggttcgc cgtctaacat
     6781 gattttaaga ccagcacgaa aatcgttgct atagtacgtt gccataaggc cctctgaaat
     6841 ttgttaattg gtagctaagc cacaaaatgg cgcatattgt aaccctaaat accccatcca
     6901 gagaagattg gttaacgcaa cttgccgatg ttgtgaccga tcctggtgaa cttctgcgtc
     6961 ttttgaatat agacgcggac gaaaaactgt tggccggacg cagcgccaaa aagctgtttg
     7021 ccctgcgtgt gccccgctca tttatcgatc gcatggagaa aggcaatccg gacgatccta
     7081 ttttgcgtca ggtacttacc tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg
     7141 acccactgga agaacagcac agcgtagtgc ctggtttgtt gcataaatac cacaaccggg
     7201 cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg ctattgcttc cgtcgccact
     7261 tcccctatgc cgaaaatcag ggcaacaagc gtaactggca gactgcactt gagtatgttg
     7321 ctgcgcatcc ggaactggac gagatgattt tctccggcgg cgatccgctg atggcgaaag
     7381 atcacgagct ggactggttg ctcacacaac tggaagccat cccgcatatc aaacgtctgc
     7441 ggattcacag ccgtctgccg attgtgatcc cggcacgtat caccgaggcg ctggttgaac
     7501 gctttgcccg ttctacgctg caaatcttgc tggtgaatca catcaatcat gccaatgagg
     7561 tagatgaaac gttccgtcag gcgatggcta aattgcgccg tgtcggtgtc accctgctta
     7621 accagagcgt tctgttacgt ggtgtgaacg ataacgcaca aacgttggca aacctgagta
     7681 atgcgttgtt cgatgccggc gtgatgccgt attacctgca tgtgctagat aaagtacagg
     7741 gcgcggcgca ttttatggtg agtgatgacg aagcacggca gattatgcgt gagttgctga
     7801 cattggtgtc gggatatctg gtgccgaaac tggcgcgaga aattggcggc gaacccagca
     7861 aaacgccgct ggatctccag ctacgccagc agtaactgat taaacaggga agttaaacat
     7921 tagcctgatg cgctacgctt atcaggccta cattgtgcat tgaaattgaa tggatttgca
     7981 atgaactgta ggccggataa ggcgtttacg ccgcatccgg caagagcaac aaacgcaagt
     8041 acagcaacct gtcattaaac tgggaaattt aatctttgcc cgttgcataa cctttaatta
     8101 ttcatataca ttgctgttgt tactctttat atagccaaca ccaata
//
LOCUS       DAZRUF010000650         5384 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.guided.248, whole genome shotgun sequence.
ACCESSION   DAZRUF010000650 DAZRUF010000000
VERSION     DAZRUF010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.guided.248"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            1..652
                     /locus_tag="ACHXOL_004619"
                     /pseudo
     CDS             1..652
                     /locus_tag="ACHXOL_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /gene="prfH"
                     /locus_tag="ACHXOL_004620"
     CDS             649..1263
                     /gene="prfH"
                     /locus_tag="ACHXOL_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR6811374.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHXOL_004621"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHXOL_004621"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR6811375.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="ACHXOL_004622"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="ACHXOL_004622"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR6811376.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="ACHXOL_004623"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="ACHXOL_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR6811377.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="ACHXOL_004624"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="ACHXOL_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR6811378.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
BASE COUNT         1307 a         1319 c         1437 g         1321 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caat
//
LOCUS       DAZRUF010000651         4465 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2302632
            SAMN43277721-rid23492333.guided.277, whole genome shotgun sequence.
ACCESSION   DAZRUF010000651 DAZRUF010000000
VERSION     DAZRUF010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277721
            Sequence Read Archive: SRR30464136
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396487.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396487.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:25:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,630
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,284
            CDSs (with protein)               :: 4,284
            Genes (RNA)                       :: 86
            rRNAs                             :: 3, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 111 of 260
            Pseudo Genes (incomplete)         :: 89 of 260
            Pseudo Genes (internal stop)      :: 88 of 260
            Pseudo Genes (multiple problems)  :: 27 of 260
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277721-rid23492333.guided.277"
                     /strain="M2302632"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2023-03-24"
                     /collected_by="FSS"
     gene            <1..392
                     /locus_tag="ACHXOL_004625"
     CDS             <1..392
                     /locus_tag="ACHXOL_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR6811379.1"
                     /translation="NASGPMAQVIGRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQ
                     ASRGSSVLVDNIRWQSYLSSMTSAEAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWF
                     RRHDGGVLKPAVLERQRKSKGVPRGEA"
     gene            379..1230
                     /gene="repC"
                     /locus_tag="ACHXOL_004626"
     CDS             379..1230
                     /gene="repC"
                     /locus_tag="ACHXOL_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="HFR6811380.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
                     YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
                     AVTADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
                     LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
                     DPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDI
                     TRPKAAG"
     gene            1538..2353
                     /gene="sul2"
                     /locus_tag="ACHXOL_004627"
     CDS             1538..2353
                     /gene="sul2"
                     /locus_tag="ACHXOL_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR6811381.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(2639..3127)
                     /gene="dfrA3"
                     /locus_tag="ACHXOL_004628"
     CDS             complement(2639..3127)
                     /gene="dfrA3"
                     /locus_tag="ACHXOL_004628"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF000019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA3"
                     /protein_id="HFR6811382.1"
                     /translation="MLISLIAALAHNNLIGKDNLIPWHLPADLRHFKAVTLGKPVVMG
                     RRTFESIGRPLPGRRNVVVSRNPQWQAEGVEVAPSLDAALALLTDCEEAMIIGGGQLY
                     AEALPRADRLYLTYIDAQLNGDTHFPDYLSLGWQELERSTHPADDKNSYACEFVTLSR
                     QR"
     gene            complement(3133..3600)
                     /locus_tag="ACHXOL_004629"
     CDS             complement(3133..3600)
                     /locus_tag="ACHXOL_004629"
                     /inference="COORDINATES: protein motif:HMM:NF024230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFR6811383.1"
                     /translation="MKELVSLALWDAFWSAIPAVGFAMLFSVPPRFLKYCAVVGALAH
                     SMRAVLMHYGLPIEWATLAASSFVSLVFVYLSRRLLAPRPVFTVACIIPMIPGKFAFN
                     TIIAVLSMNSEGVSGHLINAAIENGLKTLFILMALSFGLAIPPLIIYRNRPIV"
     gene            complement(3597..3950)
                     /locus_tag="ACHXOL_004630"
                     /pseudo
     CDS             complement(3597..3950)
                     /locus_tag="ACHXOL_004630"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
BASE COUNT          972 a         1329 c         1295 g          869 t
ORIGIN      
        1 aaaacgccag cggccccatg gcccaggtca tcggtcgcat ggaggccatc gccgccgata
       61 ccgggtgctc tatcgtgttc ctgcaccatg ccagcaaggg cgcggccatg atgggcgcag
      121 gcgaccagca gcaggccagc cggggcagct cggtactggt cgataacatc cgctggcagt
      181 cctacctgtc gagcatgacc agcgccgagg ccgaggaatg gggtgtggac gacgaccagc
      241 gccggttctt cgtccgcttc ggtgtgagca aggccaacta tggcgcaccg ttcgctgatc
      301 ggtggttcag gcggcatgac ggcggggtgc tcaagcccgc cgtgctggag aggcagcgca
      361 agagcaaggg ggtgccccgt ggtgaagcct aagaacaagc acagcctcag ccacgtccgg
      421 cacgacccgg cgcactgtct ggcccccggc ctgttccgtg ccctcaagcg gggcgagcgc
      481 aagcgcagca agctggacgt gacgtatgac tacggcgacg gcaagcggat cgagttcagc
      541 ggcccggagc cgctgggcgc tgatgatctg cgcatcctgc aagggctggt ggccatggct
      601 gggcctaatg gcctagtgct tggcccggaa cccaagaccg aaggcggacg gcagctccgg
      661 ctgttcctgg aacccaagtg ggaggccgtc accgctgatg ccatggtggt caaaggtagc
      721 tatcgggcgc tggcaaagga aatcggggca gaggtcgata gtggtggggc gctcaagcac
      781 atacaggact gcatcgagcg cctttggaag gtatccatca tcgcccagaa tggccgcaag
      841 cggcaggggt ttcggctgct gtcggagtac gccagcgacg aggcggacgg gcgcctgtac
      901 gtggccctga accccttgat cgcgcaggcc gtcatgggtg gcggccagca tgtgcgcatc
      961 agcatggacg aggtgcgggc gctggacagc gaaaccgccc gcctgctgca ccagcggctg
     1021 tgtggctgga tcgaccccgg caaaaccggc aaggcttcca tagatacctt gtgcggctat
     1081 gtctggccgt cagaggccag tggttcgacc atgcgcaagc gccgccagcg ggtgcgcgag
     1141 gcgttgccgg agctggtcgc gctgggctgg acggtaaccg agttcgcggc gggcaagtac
     1201 gacatcaccc ggcccaaggc ggcaggctga ccccccccac tctattgtaa acaagacatt
     1261 tttatctttt atattcaatg gcttattttc ctgctaattg gtaataccat gaaaaatacc
     1321 atgctcagaa aaggcttaac aatattttga aaaattgcct actgagcgct gccgcacagc
     1381 tccataggcc gctttcctgg ctttgcttcc agatgtatgc tcttctgctc ctgcagctaa
     1441 tggatcaccg caaacaggtt actcgcctgg ggattccctt tcgacccgag catccgtatg
     1501 atactcatgc tcgattatta ttattataga agcccccatg aataaatcgc tcatcatttt
     1561 cggcatcgtc aacataacct cggacagttt ctccgatgga ggccggtatc tggcgccaga
     1621 cgcagccatt gcgcaggcgc gtaagctgat ggccgagggg gcagatgtga tcgacctcgg
     1681 tccggcatcc agcaatcccg acgccgcgcc tgtttcgtcc gacacagaaa tcgcgcgtat
     1741 cgcgccggtg ctggacgcgc tcaaggcaga tggcattccc gtctcgctcg acagttatca
     1801 acccgcgacg caagcctatg ccttgtcgcg tggtgtggcc tatctcaatg atattcgcgg
     1861 ttttccagac gctgcgttct atccgcaatt ggcgaaatca tctgccaaac tcgtcgttat
     1921 gcattcggtg caagacgggc aggcagatcg gcgcgaggca cccgctggcg acatcatgga
     1981 tcacattgcg gcgttctttg acgcgcgcat cgcggcgctg acgggtgccg gtatcaaacg
     2041 caaccgcctt gtccttgatc ccggcatggg gttttttctg ggggctgctc ccgaaacctc
     2101 gctctcggtg ctggcgcggt tcgatgaatt gcggctgcgc ttcgatttgc cggtgcttct
     2161 gtctgtttcg cgcaaatcct ttctgcgcgc gctcacaggc cgtggtccgg gggatgtcgg
     2221 ggccgcgaca ctcgctgcag agcttgccgc cgccgcaggt ggagctgact tcatccgcac
     2281 acacgagccg cgccccttgc gcgacgggct ggcggtattg gcggcgctga aagaaaccgc
     2341 aagaattcgt taactgcaca ttcgggatat ttctctatat tcgcgcttca tcagaaaact
     2401 gaaggaacct ccattgaatc gaactaatat tttttttggt gcctcgcatt catgccctgg
     2461 atacgggttg tgtctggggt aatcagctca cgctctggcg atgggatgat gatgccatct
     2521 tcagcctggg ttgccccatg tacgccaaag gtgactaatc cgctcaacag agcgccaata
     2581 aaaaaaaccg ccctgttttt tggcggtttt tttgtatctg gtggtgccaa taggcggttc
     2641 agcgctgacg actcaaggta acaaattcgc aggcatagct gttcttatcg tcagcaggat
     2701 gcgttgaccg ctccaactcc tgccacccaa gcgataggta atccgggaaa tgggtatcac
     2761 cgttcaactg agcgtcaatg taggttagat acaagcgatc cgctcggggc agagcctcgg
     2821 catagagttg cccgccaccg atgatcatcg cttcctcaca gtcggttaat agcgccagag
     2881 ccgcatccag cgagggagcc acctccaccc cttcggcctg ccattgggga ttgcgactaa
     2941 cgacaacatt gcgccgtcct ggcaatggcc gcccgatcga ctcaaaggtg cgacgtccca
     3001 tcaccacagg tttccccagg gtgacagctt tgaaatgacg cagatcggca ggtagatgcc
     3061 atggaataag attatctttg ccaatcaagt tgttatgagc caaagctgca atcaaagaaa
     3121 tcaacataat cctcacacaa tcggacggtt gcggtaaata atcaacgggg gaatagccag
     3181 gccgaagctc agggccatca aaataaacag cgtcttcaat ccattctcga tagcagcatt
     3241 gatgagatgc ccactgacgc cttcgctgtt catgctcagc acggcaatga tggtattgaa
     3301 cgcgaatttg ccgggtatca tagggattat gcaggccacg gtaaagaccg ggcgcggcgc
     3361 cagcaaacga cgggacagat agacaaacac caggctgaca aaactgctgg ccgcaagcgt
     3421 ggcccactcg atcggcaacc cgtaatgcat cagcacggcg cgcatgctat gcgccagagc
     3481 cccgaccacg gcgcaatact tcaagaaacg cggtggcact gaaaacaaca tggcgaagcc
     3541 aaccgctggg atagccgacc agaatgcgtc ccacaatgcc agactaacca gctctttcat
     3601 ggctgccacc cccagactcc ggtgagcatc atcgccaaaa caataccggt tgtggcactc
     3661 agggtcagca tggttgcggt caaccagcgc gccaacccca tgttgtaata gcctttgacc
     3721 acatccagca tggcattgat caacggaaag ccaggcacca gcagcaaaac gcaggcagcc
     3781 atcgccagct ggggttgcgc cccccagtta ttcagggtag ccaggctggc gatcaaggtc
     3841 gcaacaaagg ctgtcgcgga aaagttcaac agtggactga aatggcgatg ggcaatttcc
     3901 tgacgtacaa acataccgac ggatgaagcc agaaccgtga tcaggaatac cggccagtgc
     3961 ccgtcactcc cgtactaact gtcacgaacc cctgcaataa ctgtcacgcc cccctgcaat
     4021 aactgtcacg aacccctgca ataactgtca cgcccccaaa cctgcaaacc cagcaggggc
     4081 gggggctggc ggggtgttgg aaaaatccat ccatgattat ctaagaataa tccactaggc
     4141 gcggttatca gcgcccttgt ggggcgctgc tgcccttgcc caatatgccc ggccagaggc
     4201 cggatagctg gtctattcgc tgcgctaggc tacacaccgc cccaccgctg cgcggcaggg
     4261 ggaaaggcgg gcaaagcccg ctaaacccca caccaaaccc cgcagaaata cgctggagcg
     4321 cttttagccg ctttagcggc ctttccccct acccgaaggg tgggggcgcg tgtgcagccc
     4381 cgcagggcct gtctcggtcg atcattcagc ccggctcatc cttctggcgt ggcggcagac
     4441 cgaacaaggc gcggtcgtgg tcgcg
//
